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4 ENABLED": "Yes", "PRIME-PVCAM Adapter Version": "1.3.29", "COM3-Name": "COM3", "405 AO-MaxVolts": "5.0000", "520 Shutter-Block voltage": "false", "PRIME-Timing-ClearingTimeNs": "49344000.0000", "Core-Focus": "PIEZO", "528 AO-Block voltage": "false", "TE2000-Description": "Nikon TE2000 hub", "528 AO-MinVolts": "0.0000", "COM4-Parity": "None", "Summary": { "Slices": 11, "ChContrastMin": [ 0, 0, 0, 0, 0, 0 ], "PixelAspect": 1, "SlicesFirst": true, "Interval_ms": 0, "Time": "2019-11-03 18:54:24 -0500", "Prefix": "preseq_1", "InitialPositionList": [ { "Label": "Pos0", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.165], "Focus": [16493.3], "XYStage": [ -20316.800000000003, 21203.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos1", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.165], "Focus": [16493.2], "XYStage": [ -25641.5, 20915.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos2", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.195], "Focus": [16493.2], "XYStage": [ -31960, 21643.800000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos3", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.17000000000002], "Focus": [16493.2], "XYStage": [ -37551.200000000004, 21081.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos4", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [189.485], "Focus": [16493.2], "XYStage": [ -43480.700000000004, 20946.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos5", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [189.465], "Focus": [16493.2], "XYStage": [ -49157.4, 21602 ] }, "GridColumnIndex": 0 }, { "Label": "Pos6", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [162.965], "Focus": [16493.2], "XYStage": [ -54435, 22039.800000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos7", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [133.785], "Focus": [16493.4], "XYStage": [ -59946.600000000006, 22195.600000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos8", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [109.47500000000001], "Focus": [16493.6], "XYStage": [ -66607.1, 20081.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos9", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [186.67000000000002], "Focus": [16493.4], "XYStage": [ -18583.5, -10749.6 ] }, "GridColumnIndex": 0 }, { "Label": "Pos10", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [186.685], "Focus": [16493.4], "XYStage": [ -24444.2, -11214.800000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos11", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [194.005], "Focus": [16493.4], "XYStage": [ -30335.4, -11214.900000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos12", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [168.76], "Focus": [16493.4], "XYStage": [ -36245.6, -11604 ] }, "GridColumnIndex": 0 }, { "Label": "Pos13", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [168.79500000000002], "Focus": [16493.4], "XYStage": [ -42091.700000000004, -11477.900000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos14", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [143.85], "Focus": [16493.4], "XYStage": [ -47613.600000000006, -10741 ] }, "GridColumnIndex": 0 }, { "Label": "Pos15", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [120.21000000000001], "Focus": [16493.4], "XYStage": [ -53017.700000000004, -10771 ] }, "GridColumnIndex": 0 }, { "Label": "Pos16", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [120.235], "Focus": [16493.2], "XYStage": [ -58940.100000000006, -10881.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos17", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [120.19], "Focus": [16493.3], "XYStage": [ -65459.200000000004, -11020.2 ] }, "GridColumnIndex": 0 } ], "z-step_um": 10, "GridRow": 0, "BitDepth": 16, "Depth": 2, "Channels": 6, "Source": "Micro-Manager", "ChColors": [ -16711681, -256, -65281, -1, -1, -16776961 ], "MetadataVersion": 10, "KeepShutterOpenChannels": true, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536, 65536 ], "CustomIntervals_ms": [], "Height": 1200, "MicroManagerVersion": "1.4.23 20180219", "GridColumn": 0, "UUID": "7cf027ba-04ba-452b-ab24-b2e9adcb08eb", "PixelType": "GRAY16", "Width": 1200, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\Yan\\20191103-YC107-S4S5-miseq", "Frames": 1, "Comment": "", "UserName": "Nikon", "PixelSize_um": 1.1, "ROI": [ 0, 0, 1200, 1200 ], "Date": "2019-11-03", "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC", "P-DAPI-high" ], "KeepShutterOpenSlices": true, "IJType": 1, "ComputerName": "ZadorConfocal2", "Positions": 18, "PositionIndex": 14, "CameraTimeout": 20000, "TimeFirst": false }, "640 AO-Name": "AnalogIO", "Vincent-D3-Command": "Close" } }