{ "Summary": { "Slices": 11, "ChContrastMin": [ 0, 0, 0, 0, 0, 0 ], "PixelAspect": 1, "SlicesFirst": true, "Interval_ms": 0, "Time": "2019-12-17 03:59:33 -0500", "Prefix": "seq_7", "InitialPositionList": [ { "Label": "Pos0", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [149.91], "Focus": [16657.7], "XYStage": [ 71629.7, 19152.9 ] }, "GridColumnIndex": 0 }, { "Label": "Pos1", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [190.82], "Focus": [16485.4], "XYStage": [ 65702.3, 19396.600000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos2", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [194.69], "Focus": [16446.8], "XYStage": [ 59880.5, 19007.8 ] }, "GridColumnIndex": 0 }, { "Label": "Pos3", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [145.04], "Focus": [16446.6], "XYStage": [ 53616.9, 18818.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos4", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [169.57500000000002], "Focus": [16446.5], "XYStage": [ 47662.8, 18817.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos5", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [131.805], "Focus": [16446.4], "XYStage": [ 41918.8, 18298.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos6", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [113.145], "Focus": [16446.5], "XYStage": [ 36129.9, 18895.8 ] }, "GridColumnIndex": 0 }, { "Label": "Pos7", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [141.005], "Focus": [16446.6], "XYStage": [ 30299.100000000002, 18895.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos8", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [140.97], "Focus": [16446.5], "XYStage": [ 23952.100000000002, 19156 ] }, "GridColumnIndex": 0 }, { "Label": "Pos9", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [184.57500000000002], "Focus": [16677.9], "XYStage": [ 72866.8, -14809.300000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos10", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [167.95000000000002], "Focus": [16557.9], "XYStage": [ 66515.6, -14371.300000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos11", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [168], "Focus": [16618.9], "XYStage": [ 59724.3, -13964.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos12", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [203.205], "Focus": [16779.6], "XYStage": [ 53862.3, -13494.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos13", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [203.20000000000002], "Focus": [16749], "XYStage": [ 48108.3, -13805.400000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos14", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [203.19], "Focus": [16675.9], "XYStage": [ 41645, -13804.900000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos15", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [203.21], "Focus": [16675.7], "XYStage": [ 35352.6, -13805.6 ] }, "GridColumnIndex": 0 }, { "Label": "Pos16", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [203.225], "Focus": [16675.8], "XYStage": [ 29298, -13447.400000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos17", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [203.215], "Focus": [16675.8], "XYStage": [ 23269.100000000002, -13012.300000000001 ] }, "GridColumnIndex": 0 } ], "z-step_um": 10, "GridRow": 0, "BitDepth": 16, "Depth": 2, "Channels": 6, "Source": "Micro-Manager", "ChColors": [ -16711681, -256, -65281, -1, -1, -16776961 ], "MetadataVersion": 10, "KeepShutterOpenChannels": true, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536, 65536 ], "CustomIntervals_ms": [], "Height": 1200, "MicroManagerVersion": "1.4.23 20180219", "GridColumn": 0, "UUID": "3ad8801b-5699-40d6-813b-4ad840863e38", "PixelType": "GRAY16", "Width": 1200, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\Yan\\20191216-YC113-S11S12-miseq", "Frames": 1, "Comment": "", "UserName": "Nikon", "PixelSize_um": 1.1, "ROI": [ 0, 0, 1200, 1200 ], "Date": "2019-12-17", "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC", "P-DAPI-high" ], "KeepShutterOpenSlices": true, "IJType": 1, "ComputerName": "ZadorConfocal2", "Positions": 18, "PositionIndex": 17, "CameraTimeout": 20000, "TimeFirst": false }, "FrameKey-0-0-0": { "COM4-StopBits": "1", "528 AO-MaxVolts": "5.0000", "XLIGHT Excitation Wheel-State": "1", "470 Shutter-Name": "AnalogIO", "Core-Shutter": "520", "555 AO-Demo": "No", "XLIGHT Dichroic-State": "2", "XYStage-StepSize-Y": "0.1000", "ZPositionUm": 153.215, "XYStage-StepSize-X": "0.1000", "PRIME-FirmwareVersion": "20.28", "PRIME-ClearCycles": "2", "520-Name": "DA Shutter", "405 Shutter-Name": "AnalogIO", "PIEZO-StepSize": "0.0050", "PRIME-ClearMode": "Pre-Exposure", "470 AO-MinVolts": "0.0000", "405 AO-SupportsTriggering": "1", "CameraChannelIndex": 0, "XYStage-StartSpeed": "500.0000", "COM9-StopBits": "2", "405 AO-Description": "NI DAC", "COM4-DelayBetweenCharsMs": "0.0000", "445 AO-Block voltage": "false", "520 AO-TriggerInputLine": "/Dev1/PFI0", "555 AO-Description": "NI DAC", "520 AO-TriggerSampleRate": "100000", "COM4-Verbose": "1", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM4-Name": "COM4", "405 Shutter-Volts": "0.0000", "Vincent-D3-Port": "COM1", "PRIME-PP 4 MIN ADU AFFECTED": "200", "470 Shutter-MinVolts": "0.0000", "PRIME-PP 3 ENABLED": "Yes", "470 AO-Description": "NI DAC", "PRIME-Description": "PVCAM API device adapter", "520 Shutter-MaxVolts": "5.0000", "520 Shutter-Volts": "5.0000", "528 AO-Sequenceable": "1", "PRIME-Binning": "1x1", "555 Shutter-MaxVolts": "5.0000", "528 Shutter-TriggerSequenceLength": "1000", "405 Shutter-Block voltage": "false", "XLIGHT Spin motor-State": "1", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "Vincent-D3-Time to open (ms)": "35.0000", "Vincent-D3-Time to close (ms)": "35.0000", "445 AO-TriggerSampleRate": "100000", "640 Shutter-IOChannel": "Dev1/ao14", "555 AO-Sequenceable": "1", "520 AO-Name": "AnalogIO", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "470 AO-Block voltage": "false", "COM1-Verbose": "1", "COM9-AnswerTimeout": "10000.0000", "528 Shutter-Name": "AnalogIO", "PRIME-Trigger Timeout (secs)": "10", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "NextFrame": 0, "470 AO-TriggerSequenceLength": "1000", "PRIME-TransposeXY": "0", "445 AO-TriggerSequenceLength": "1000", "COM1-Name": "COM1", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "COM9-DataBits": "8", "COM1-AnswerTimeout": "500.0000", "Camera": "", "TE2000-Version": "V2.50-V2.50-V2.10", "528 Shutter-Volts": "0.0000", "XLIGHT Hub-Name": "XLIGHT Hub", "PIEZO-Description": "Ludl stage driver adapter", "PositionName": "Pos17", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSampleRate": "100000", "PRIME-FanSpeedSetpoint": "High", "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "405 AO-TriggerSampleRate": "100000", "470 Shutter-Block voltage": "false", "470-State": "0", "COM3-Parity": "None", "405 AO-Volts": "0.0000", "PRIME-TransposeMirrorY": "0", "PRIME-ChipName": "GS144BSI", "470 AO-Volts": "0.0000", "PRIME-TransposeMirrorX": "0", "445 Shutter-SupportsTriggering": "0", "470 Shutter-SupportsTriggering": "0", "XLIGHT Hub-Description": "XLIGHT hub", "PRIME-PreampOffLimit": "10000", "470 AO-Sequenceable": "1", "528 Shutter-MaxVolts": "5.0000", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "Core-XYStage": "XYStage", "470 AO-TriggerSampleRate": "100000", "PRIME-Timing-PreTriggerDelayNs": "24672000.0000", "528-DA Device": "528 Shutter", "Focus-Description": "Nikon TE2000 focus adapter", "FilterBlock-State": "5", "520 AO-Demo": "No", "COM3-DelayBetweenCharsMs": "0.0000", "470 Shutter-Volts": "0.0000", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "PRIME-PP 2 THRESHOLD": "125", "PRIME-PP 4 THRESHOLD": "75", "XYStage-Acceleration": "4.0000", "470 Shutter-Demo": "No", "PRIME-Name": "PMPCIECam00", "640-DA Device": "640 Shutter", "405 AO-Block voltage": "false", "ElapsedTime-ms": 716181, "PRIME-Offset": "100", "445 AO-IOChannel": "Dev1/ao1", "640 AO-MaxVolts": "5.0000", "PositionIndex": 17, "PRIME-PP 1 ENABLED": "Yes", "COM1-BaudRate": "9600", "640-State": "0", "COM3-Verbose": "1", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "LudlController-TransmissionDelay": "0", "PRIME-FullWellCapacity": "62000", "PRIME-SMARTStreamingEnabled": "No", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "640 Shutter-Description": "NI DAC", "528-Description": "DA device that is used as a shutter", "COM4-Description": "Serial port driver (boost:asio)", "405 Shutter-Description": "NI DAC", "TE2000-Name": "TE2000", "528 Shutter-Description": "NI DAC", "COM9-Description": "Serial port driver (boost:asio)", "LudlController-Name": "LudlController", "445 Shutter-TriggerSequenceLength": "1000", "PRIME-SerialNumber": "A18B203008", "445 AO-Volts": "0.0000", "PRIME-X-dimension": "1200", "PRIME-ShutterCloseDelay": "0", "XLIGHT Disk slider-State": "2", "Position": "Default", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 AO-IOChannel": "Dev1/ao5", "520 AO-IOChannel": "Dev1/ao4", "528 AO-TriggerSequenceLength": "1000", "405 Shutter-MaxVolts": "5.0000", "470-DA Device": "470 Shutter", "PRIME-FTCapable": "No", "Core-ChannelGroup": "Channels", "555 AO-SupportsTriggering": "0", "PRIME-Y-dimension": "1200", "Frame": 0, "555 Shutter-Sequenceable": "1", "PRIME-Timing-ExposureTimeNs": "499998000.0000", "Core-ImageProcessor": "", "528-State": "0", "520 AO-Block voltage": "false", "XLIGHT Excitation Wheel-Label": "CFP/YFP", "640 Shutter-Demo": "No", "COM3-StopBits": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "520 Shutter-IOChannel": "Dev1/ao11", "Width": 1200, "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-SupportsTriggering": "0", "640 AO-TriggerSampleRate": "100000", "520-Description": "DA device that is used as a shutter", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "405 Shutter-Sequenceable": "1", "405 Shutter-SupportsTriggering": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-TriggerSampleRate": "100000", "640 Shutter-MaxVolts": "5.0000", "555 AO-MaxVolts": "5.0000", "520 AO-TriggerSequenceLength": "1000", "COM3-BaudRate": "9600", "COM9-BaudRate": "9600", "Vincent-D3-Channel #": "Ch. #1", "PRIME-PMode": "Normal", "OpticalPath-Label": "Left(100)", "BitDepth": 16, "555 AO-TriggerSequenceLength": "1000", "445 Shutter-Description": "NI DAC", "405 AO-Sequenceable": "1", "PRIME-AcquisitionMethod": "Callbacks", "555 AO-Name": "AnalogIO", "PRIME-ShutterMode": "Pre-Exposure", "COM3-Handshaking": "Off", "445 AO-SupportsTriggering": "0", "ROI": "0-0-1200-1200", "COM9-Verbose": "1", "PRIME-Color": "OFF", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "PRIME-ShutterOpenDelay": "0", "COM1-Parity": "None", "Slice": 0, "SlicePosition": -50, "COM9-DelayBetweenCharsMs": "0.0000", "555-Name": "DA Shutter", "PIEZO-LudlSingleAxisName": "B", "XLIGHT Emission Wheel-Label": "565/24(G)", "640 Shutter-Name": "AnalogIO", "640 AO-Sequenceable": "1", "520 Shutter-Demo": "No", "528 Shutter-Demo": "No", "470-Name": "DA Shutter", "520-DA Device": "520 Shutter", "XYStage-Description": "Ludl XY stage driver adapter", "445 Shutter-MinVolts": "0.0000", "528 AO-TriggerSampleRate": "100000", "405 AO-MinVolts": "0.0000", "405 Shutter-TriggerSequenceLength": "1000", "Height": 1200, "555 AO-Block voltage": "false", "640 Shutter-Volts": "0.0000", "PixelType": "GRAY16", "445 Shutter-Block voltage": "false", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "520 Shutter-TriggerSequenceLength": "1000", "COM9-Name": "COM9", "Core-SLM": "", "555 AO-Volts": "0.0000", "555 Shutter-Block voltage": "false", "XYStage-Speed": "1800.0000", "520 AO-MaxVolts": "5.0000", "528 Shutter-SupportsTriggering": "0", "COM1-Description": "Serial port driver (boost:asio)", "PRIME-MetadataEnabled": "No", "520 Shutter-MinVolts": "0.0000", "470 Shutter-Sequenceable": "1", "PRIME-PreampDelay": "0", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-MinVolts": "0.0000", "ChannelIndex": 0, "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Core-AutoShutter": "1", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "Exposure-ms": 320, "PRIME-PP 2 ENABLED": "Yes", "640 Shutter-TriggerSampleRate": "100000", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "470 Shutter-IOChannel": "Dev1/ao10", "405 AO-Demo": "No", "PixelSizeUm": 1.1, "470 AO-Demo": "No", "470 Shutter-MaxVolts": "5.0000", "Vincent-D3-Name": "Vincent-D3", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FileName": "img_000000000_Il-G_000.tif", "LudlController-Reset": "Operate", "PRIME-Exposure": "320.00", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "445-Name": "DA Shutter", "555 Shutter-Name": "AnalogIO", "520 Shutter-TriggerSampleRate": "100000", "445 Shutter-IOChannel": "Dev1/ao9", "PRIME-TransposeCorrection": "0", "XYStage-StepSize": "0.1000", "445-Description": "DA device that is used as a shutter", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 AO-IOChannel": "Dev1/ao7", "520 Shutter-Name": "AnalogIO", "COM3-DataBits": "8", "640 AO-Volts": "0.0000", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "555 Shutter-IOChannel": "Dev1/ao13", "WaitInterval": null, "520 Shutter-Sequenceable": "1", "YPositionUm": -13012.300000000001, "445-State": "0", "445-DA Device": "445 Shutter", "PRIME-CircularBufferAutoSize": "ON", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "555 AO-TriggerSampleRate": "100000", "445 Shutter-Volts": "0.0000", "XLIGHT Hub-Port": "COM3", "Core-AutoFocus": "", "COM4-Handshaking": "Off", "528 Shutter-MinVolts": "0.0000", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "445 Shutter-Name": "AnalogIO", "528 Shutter-Sequenceable": "1", "XYStage-TransposeMirrorX": "0", "XYStage-TransposeMirrorY": "0", "PRIME-ReadoutRate": "100MHz 16bit", "XYStage-Name": "Stage", "640 AO-SupportsTriggering": "0", "640-Name": "DA Shutter", "640 Shutter-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "640 AO-Block voltage": "false", "445 Shutter-Sequenceable": "1", "470 AO-MaxVolts": "5.0000", "COM4-BaudRate": "9600", "470-Description": "DA device that is used as a shutter", "520 Shutter-Description": "NI DAC", "PRIME-CCDTemperature": "-14.9200", "Time": "2019-12-17 03:59:33 -0500", "640 AO-TriggerSequenceLength": "1000", "470 AO-Name": "AnalogIO", "XLIGHT Disk slider-Label": "Disk Pos 40um", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "Binning": "1x1", "TE2000-ModelType": "1", "COM3-AnswerTimeout": "500.0000", "555 Shutter-SupportsTriggering": "0", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "TE2000-Port": "COM4", "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Description": "NI DAC", "COM4-AnswerTimeout": "500.0000", "COM1-DelayBetweenCharsMs": "0.0000", "FilterBlock-Label": "Confocal", "445 AO-Name": "AnalogIO", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-Timing-ReadoutTimeNs": "24672000.0000", "528 AO-Demo": "No", "520 AO-Sequenceable": "1", "555-Description": "DA device that is used as a shutter", "405-DA Device": "405 Shutter", "405 AO-TriggerSequenceLength": "1000", "640 AO-MinVolts": "0.0000", "640 Shutter-Block voltage": "false", "FrameIndex": 0, "PRIME-Port": "CMOS", "555 AO-MinVolts": "0.0000", "COM3-Description": "Serial port driver (boost:asio)", "528 AO-Description": "NI DAC", "555 AO-IOChannel": "Dev1/ao6", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "555 AO-TriggerInputLine": "/Dev1/PFI0", "PRIME-ActualInterval-ms": "84.6200", "LudlController-Port": "COM9", "445 Shutter-Demo": "No", "PRIME-Trigger-Expose Out-Mux": "1", "SliceIndex": 0, "PRIME-CCDTemperatureSetPoint": "-15.0000", "405 Shutter-MinVolts": "0.0000", "PRIME-ExposeOutMode": "First Row", "AxisPositions": null, "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Volts": "0.0000", "528-Name": "DA Shutter", "555-State": "0", "XLIGHT Dichroic-Label": "CFP/YFP", "445 AO-Sequenceable": "1", "405-Name": "DA Shutter", "405 AO-IOChannel": "Dev1/ao0", "Core-Camera": "PRIME", "Focus-Name": "Focus", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "405 Shutter-IOChannel": "Dev1/ao8", "640 AO-Demo": "No", "640 Shutter-SupportsTriggering": "0", "445 AO-Demo": "No", "555-DA Device": "555 Shutter", "470 Shutter-Description": "NI DAC", "528 AO-Volts": "0.0000", "COM1-DataBits": "8", "Vincent-D3-Address": "x", "520 AO-SupportsTriggering": "0", "405-Description": "DA device that is used as a shutter", "640 Shutter-MinVolts": "0.0000", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "405 AO-Name": "AnalogIO", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "640 AO-TriggerInputLine": "/Dev1/PFI0", "405 AO-TriggerInputLine": "/Dev1/PFI0", "640-Description": "DA device that is used as a shutter", "445 Shutter-TriggerSampleRate": "100000", "470 Shutter-TriggerSequenceLength": "1000", "COM1-StopBits": "1", "520 Shutter-SupportsTriggering": "0", "405-State": "0", "Core-Initialize": "1", "XLIGHT Emission Wheel-State": "3", "520 AO-Description": "NI DAC", "528 AO-TriggerInputLine": "/Dev1/PFI0", "445 Shutter-MaxVolts": "5.0000", "COM9-Handshaking": "Off", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "XLIGHT Spin motor-Label": "On (spinning)", "PRIME-PP 5 ENABLED": "No", "UUID": "7d3612d7-cafd-48d4-a942-dcc42c26c75e", "PRIME-TriggerMode": "Internal Trigger", "COM1-Handshaking": "Off", "PRIME-Timing-PostTriggerDelayNs": "49344000.0000", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "Focus-Position": "16732.9000", "528 Shutter-Block voltage": "false", "Channel": "Il-G", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "555 Shutter-Demo": "No", "405 Shutter-Demo": "No", "528 AO-Name": "AnalogIO", "520-State": "1", "Core-Galvo": "", "405 Shutter-TriggerSampleRate": "100000", "Focus-FocusStepSize_(nm)": "100", "PRIME-PixelType": "16bit", "COM4-DataBits": "8", "470 AO-IOChannel": "Dev1/ao2", "520 AO-MinVolts": "0.0000", "COM9-Parity": "None", "PRIME-CircularBufferEnabled": "ON", "PRIME-PP 6 Reset": "No", "640 AO-Description": "NI DAC", "Source": "PRIME", "445 AO-MinVolts": "0.0000", "PIEZO-Name": "Stage", "445 AO-Description": "NI DAC", "PRIME-CircularBufferFrameCount": "46", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "520 AO-Volts": "4.5000", "528 Shutter-TriggerSampleRate": "100000", "XPositionUm": 23269.100000000002, "OpticalPath-State": "4", "PRIME-PP 3 THRESHOLD": "80", "PRIME-PVCAM Version": "3.7.1", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Gain": "1-HDR", "640 Shutter-TriggerSequenceLength": "1000", "528 AO-SupportsTriggering": "0", "445 AO-TriggerInputLine": "/Dev1/PFI0", "OpticalPath-Name": "OpticalPath", "PRIME-PP 4 ENABLED": "Yes", "PRIME-PVCAM Adapter Version": "1.3.29", "COM3-Name": "COM3", "405 AO-MaxVolts": "5.0000", "520 Shutter-Block voltage": "false", "PRIME-Timing-ClearingTimeNs": "49344000.0000", "Core-Focus": "PIEZO", "528 AO-Block voltage": "false", "TE2000-Description": "Nikon TE2000 hub", "528 AO-MinVolts": "0.0000", "COM4-Parity": "None", "Summary": { "Slices": 11, "ChContrastMin": [ 0, 0, 0, 0, 0, 0 ], "PixelAspect": 1, "SlicesFirst": true, "Interval_ms": 0, "Time": "2019-12-17 03:59:33 -0500", "Prefix": "seq_7", "InitialPositionList": [ { "Label": "Pos0", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [149.91], "Focus": [16657.7], "XYStage": [ 71629.7, 19152.9 ] }, "GridColumnIndex": 0 }, { "Label": "Pos1", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [190.82], "Focus": [16485.4], "XYStage": [ 65702.3, 19396.600000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos2", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [194.69], "Focus": [16446.8], "XYStage": [ 59880.5, 19007.8 ] }, "GridColumnIndex": 0 }, { "Label": "Pos3", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [145.04], "Focus": [16446.6], "XYStage": [ 53616.9, 18818.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos4", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [169.57500000000002], "Focus": [16446.5], "XYStage": [ 47662.8, 18817.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos5", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [131.805], "Focus": [16446.4], "XYStage": [ 41918.8, 18298.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos6", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [113.145], "Focus": [16446.5], "XYStage": [ 36129.9, 18895.8 ] }, "GridColumnIndex": 0 }, { "Label": "Pos7", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [141.005], "Focus": [16446.6], "XYStage": [ 30299.100000000002, 18895.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos8", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [140.97], "Focus": [16446.5], "XYStage": [ 23952.100000000002, 19156 ] }, "GridColumnIndex": 0 }, { "Label": "Pos9", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [184.57500000000002], "Focus": [16677.9], "XYStage": [ 72866.8, -14809.300000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos10", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [167.95000000000002], "Focus": [16557.9], "XYStage": [ 66515.6, -14371.300000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos11", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [168], "Focus": [16618.9], "XYStage": [ 59724.3, -13964.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos12", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [203.205], "Focus": [16779.6], "XYStage": [ 53862.3, -13494.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos13", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [203.20000000000002], "Focus": [16749], "XYStage": [ 48108.3, -13805.400000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos14", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [203.19], "Focus": [16675.9], "XYStage": [ 41645, -13804.900000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos15", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [203.21], "Focus": [16675.7], "XYStage": [ 35352.6, -13805.6 ] }, "GridColumnIndex": 0 }, { "Label": "Pos16", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [203.225], "Focus": [16675.8], "XYStage": [ 29298, -13447.400000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos17", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [203.215], "Focus": [16675.8], "XYStage": [ 23269.100000000002, -13012.300000000001 ] }, "GridColumnIndex": 0 } ], "z-step_um": 10, "GridRow": 0, "BitDepth": 16, "Depth": 2, "Channels": 6, "Source": "Micro-Manager", "ChColors": [ -16711681, -256, -65281, -1, -1, -16776961 ], "MetadataVersion": 10, "KeepShutterOpenChannels": true, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536, 65536 ], "CustomIntervals_ms": [], "Height": 1200, "MicroManagerVersion": "1.4.23 20180219", "GridColumn": 0, "UUID": "3ad8801b-5699-40d6-813b-4ad840863e38", "PixelType": "GRAY16", "Width": 1200, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\Yan\\20191216-YC113-S11S12-miseq", "Frames": 1, "Comment": "", "UserName": "Nikon", "PixelSize_um": 1.1, "ROI": [ 0, 0, 1200, 1200 ], "Date": "2019-12-17", "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC", "P-DAPI-high" ], "KeepShutterOpenSlices": true, "IJType": 1, "ComputerName": "ZadorConfocal2", "Positions": 18, "PositionIndex": 17, "CameraTimeout": 20000, "TimeFirst": false }, "640 AO-Name": "AnalogIO", "Vincent-D3-Command": "Close" }, "FrameKey-0-0-1": { "COM4-StopBits": "1", "528 AO-MaxVolts": "5.0000", "XLIGHT Excitation Wheel-State": "1", "470 Shutter-Name": "AnalogIO", "Core-Shutter": "520", "555 AO-Demo": "No", "XLIGHT Dichroic-State": "2", "XYStage-StepSize-Y": "0.1000", "ZPositionUm": 163.215, "XYStage-StepSize-X": "0.1000", "PRIME-FirmwareVersion": "20.28", "PRIME-ClearCycles": "2", "520-Name": "DA Shutter", "405 Shutter-Name": "AnalogIO", "PIEZO-StepSize": "0.0050", "PRIME-ClearMode": "Pre-Exposure", "470 AO-MinVolts": "0.0000", "405 AO-SupportsTriggering": "1", "CameraChannelIndex": 0, "XYStage-StartSpeed": "500.0000", "COM9-StopBits": "2", "405 AO-Description": "NI DAC", "COM4-DelayBetweenCharsMs": "0.0000", "445 AO-Block voltage": "false", "520 AO-TriggerInputLine": "/Dev1/PFI0", "555 AO-Description": "NI DAC", "520 AO-TriggerSampleRate": "100000", "COM4-Verbose": "1", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM4-Name": "COM4", "405 Shutter-Volts": "0.0000", "Vincent-D3-Port": "COM1", "PRIME-PP 4 MIN ADU AFFECTED": "200", "470 Shutter-MinVolts": "0.0000", "PRIME-PP 3 ENABLED": "Yes", "470 AO-Description": "NI DAC", "PRIME-Description": "PVCAM API device adapter", "520 Shutter-MaxVolts": "5.0000", "520 Shutter-Volts": "5.0000", "528 AO-Sequenceable": "1", "PRIME-Binning": "1x1", "555 Shutter-MaxVolts": "5.0000", "528 Shutter-TriggerSequenceLength": "1000", "405 Shutter-Block voltage": "false", "XLIGHT Spin motor-State": "1", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "Vincent-D3-Time to open (ms)": "35.0000", "Vincent-D3-Time to close (ms)": "35.0000", "445 AO-TriggerSampleRate": "100000", "640 Shutter-IOChannel": "Dev1/ao14", "555 AO-Sequenceable": "1", "520 AO-Name": "AnalogIO", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "470 AO-Block voltage": "false", "COM1-Verbose": "1", "COM9-AnswerTimeout": "10000.0000", "528 Shutter-Name": "AnalogIO", "PRIME-Trigger Timeout (secs)": "10", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "NextFrame": 0, "470 AO-TriggerSequenceLength": "1000", "PRIME-TransposeXY": "0", "445 AO-TriggerSequenceLength": "1000", "COM1-Name": "COM1", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "COM9-DataBits": "8", "COM1-AnswerTimeout": "500.0000", "Camera": "", "TE2000-Version": "V2.50-V2.50-V2.10", "528 Shutter-Volts": "0.0000", "XLIGHT Hub-Name": "XLIGHT Hub", "PIEZO-Description": "Ludl stage driver adapter", "PositionName": "Pos17", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSampleRate": "100000", "PRIME-FanSpeedSetpoint": "High", "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "405 AO-TriggerSampleRate": "100000", "470 Shutter-Block voltage": "false", "470-State": "0", "COM3-Parity": "None", "405 AO-Volts": "0.0000", "PRIME-TransposeMirrorY": "0", "PRIME-ChipName": "GS144BSI", "470 AO-Volts": "0.0000", "PRIME-TransposeMirrorX": "0", "445 Shutter-SupportsTriggering": "0", "470 Shutter-SupportsTriggering": "0", "XLIGHT Hub-Description": "XLIGHT hub", "PRIME-PreampOffLimit": "10000", "470 AO-Sequenceable": "1", "528 Shutter-MaxVolts": "5.0000", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "Core-XYStage": "XYStage", "470 AO-TriggerSampleRate": "100000", "PRIME-Timing-PreTriggerDelayNs": "24672000.0000", "528-DA Device": "528 Shutter", "Focus-Description": "Nikon TE2000 focus adapter", "FilterBlock-State": "5", "520 AO-Demo": "No", "COM3-DelayBetweenCharsMs": "0.0000", "470 Shutter-Volts": "0.0000", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "PRIME-PP 2 THRESHOLD": "125", "PRIME-PP 4 THRESHOLD": "75", "XYStage-Acceleration": "4.0000", "470 Shutter-Demo": "No", "PRIME-Name": "PMPCIECam00", "640-DA Device": "640 Shutter", "405 AO-Block voltage": "false", "ElapsedTime-ms": 716789, "PRIME-Offset": "100", "445 AO-IOChannel": "Dev1/ao1", "640 AO-MaxVolts": "5.0000", "PositionIndex": 17, "PRIME-PP 1 ENABLED": "Yes", "COM1-BaudRate": "9600", "640-State": "0", "COM3-Verbose": "1", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "LudlController-TransmissionDelay": "0", "PRIME-FullWellCapacity": "62000", "PRIME-SMARTStreamingEnabled": "No", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "640 Shutter-Description": "NI DAC", "528-Description": "DA device that is used as a shutter", "COM4-Description": "Serial port driver (boost:asio)", "405 Shutter-Description": "NI DAC", "TE2000-Name": "TE2000", "528 Shutter-Description": "NI DAC", "COM9-Description": "Serial port driver (boost:asio)", "LudlController-Name": "LudlController", "445 Shutter-TriggerSequenceLength": "1000", "PRIME-SerialNumber": "A18B203008", "445 AO-Volts": "0.0000", "PRIME-X-dimension": "1200", "PRIME-ShutterCloseDelay": "0", "XLIGHT Disk slider-State": "2", "Position": "Default", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 AO-IOChannel": "Dev1/ao5", "520 AO-IOChannel": "Dev1/ao4", "528 AO-TriggerSequenceLength": "1000", "405 Shutter-MaxVolts": "5.0000", "470-DA Device": "470 Shutter", "PRIME-FTCapable": "No", "Core-ChannelGroup": "Channels", "555 AO-SupportsTriggering": "0", "PRIME-Y-dimension": "1200", "Frame": 0, "555 Shutter-Sequenceable": "1", "PRIME-Timing-ExposureTimeNs": "499998000.0000", "Core-ImageProcessor": "", "528-State": "0", "520 AO-Block voltage": "false", "XLIGHT Excitation Wheel-Label": "CFP/YFP", "640 Shutter-Demo": "No", "COM3-StopBits": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "520 Shutter-IOChannel": "Dev1/ao11", "Width": 1200, "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-SupportsTriggering": "0", "640 AO-TriggerSampleRate": "100000", "520-Description": "DA device that is used as a shutter", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "405 Shutter-Sequenceable": "1", "405 Shutter-SupportsTriggering": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-TriggerSampleRate": "100000", "640 Shutter-MaxVolts": "5.0000", "555 AO-MaxVolts": "5.0000", "520 AO-TriggerSequenceLength": "1000", "COM3-BaudRate": "9600", "COM9-BaudRate": "9600", "Vincent-D3-Channel #": "Ch. #1", "PRIME-PMode": "Normal", "OpticalPath-Label": "Left(100)", "BitDepth": 16, "555 AO-TriggerSequenceLength": "1000", "445 Shutter-Description": "NI DAC", "405 AO-Sequenceable": "1", "PRIME-AcquisitionMethod": "Callbacks", "555 AO-Name": "AnalogIO", "PRIME-ShutterMode": "Pre-Exposure", "COM3-Handshaking": "Off", "445 AO-SupportsTriggering": "0", "ROI": "0-0-1200-1200", "COM9-Verbose": "1", "PRIME-Color": "OFF", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "PRIME-ShutterOpenDelay": "0", "COM1-Parity": "None", "Slice": 1, "SlicePosition": -40, "COM9-DelayBetweenCharsMs": "0.0000", "555-Name": "DA Shutter", "PIEZO-LudlSingleAxisName": "B", "XLIGHT Emission Wheel-Label": "565/24(G)", "640 Shutter-Name": "AnalogIO", "640 AO-Sequenceable": "1", "520 Shutter-Demo": "No", "528 Shutter-Demo": "No", "470-Name": "DA Shutter", "520-DA Device": "520 Shutter", "XYStage-Description": "Ludl XY stage driver adapter", "445 Shutter-MinVolts": "0.0000", "528 AO-TriggerSampleRate": "100000", "405 AO-MinVolts": "0.0000", "405 Shutter-TriggerSequenceLength": "1000", "Height": 1200, "555 AO-Block voltage": "false", "640 Shutter-Volts": "0.0000", "PixelType": "GRAY16", "445 Shutter-Block voltage": "false", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "520 Shutter-TriggerSequenceLength": "1000", "COM9-Name": "COM9", "Core-SLM": "", "555 AO-Volts": "0.0000", "555 Shutter-Block voltage": "false", "XYStage-Speed": "1800.0000", "520 AO-MaxVolts": "5.0000", "528 Shutter-SupportsTriggering": "0", "COM1-Description": "Serial port driver (boost:asio)", "PRIME-MetadataEnabled": "No", "520 Shutter-MinVolts": "0.0000", "470 Shutter-Sequenceable": "1", "PRIME-PreampDelay": "0", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-MinVolts": "0.0000", "ChannelIndex": 0, "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Core-AutoShutter": "1", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "Exposure-ms": 320, "PRIME-PP 2 ENABLED": "Yes", "640 Shutter-TriggerSampleRate": "100000", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "470 Shutter-IOChannel": "Dev1/ao10", "405 AO-Demo": "No", "PixelSizeUm": 1.1, "470 AO-Demo": "No", "470 Shutter-MaxVolts": "5.0000", "Vincent-D3-Name": "Vincent-D3", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FileName": "img_000000000_Il-G_001.tif", "LudlController-Reset": "Operate", "PRIME-Exposure": "320.00", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "445-Name": "DA Shutter", "555 Shutter-Name": "AnalogIO", "520 Shutter-TriggerSampleRate": "100000", "445 Shutter-IOChannel": "Dev1/ao9", "PRIME-TransposeCorrection": "0", "XYStage-StepSize": "0.1000", "445-Description": "DA device that is used as a shutter", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 AO-IOChannel": "Dev1/ao7", "520 Shutter-Name": "AnalogIO", "COM3-DataBits": "8", "640 AO-Volts": "0.0000", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "555 Shutter-IOChannel": "Dev1/ao13", "WaitInterval": null, "520 Shutter-Sequenceable": "1", "YPositionUm": -13012.300000000001, "445-State": "0", "445-DA Device": "445 Shutter", "PRIME-CircularBufferAutoSize": "ON", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "555 AO-TriggerSampleRate": "100000", "445 Shutter-Volts": "0.0000", "XLIGHT Hub-Port": "COM3", "Core-AutoFocus": "", "COM4-Handshaking": "Off", "528 Shutter-MinVolts": "0.0000", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "445 Shutter-Name": "AnalogIO", "528 Shutter-Sequenceable": "1", "XYStage-TransposeMirrorX": "0", "XYStage-TransposeMirrorY": "0", "PRIME-ReadoutRate": "100MHz 16bit", "XYStage-Name": "Stage", "640 AO-SupportsTriggering": "0", "640-Name": "DA Shutter", "640 Shutter-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "640 AO-Block voltage": "false", "445 Shutter-Sequenceable": "1", "470 AO-MaxVolts": "5.0000", "COM4-BaudRate": "9600", "470-Description": "DA device that is used as a shutter", "520 Shutter-Description": "NI DAC", "PRIME-CCDTemperature": "-14.9200", "Time": "2019-12-17 03:59:33 -0500", "640 AO-TriggerSequenceLength": "1000", "470 AO-Name": "AnalogIO", "XLIGHT Disk slider-Label": "Disk Pos 40um", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "Binning": "1x1", "TE2000-ModelType": "1", "COM3-AnswerTimeout": "500.0000", "555 Shutter-SupportsTriggering": "0", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "TE2000-Port": "COM4", "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Description": "NI DAC", "COM4-AnswerTimeout": "500.0000", "COM1-DelayBetweenCharsMs": "0.0000", "FilterBlock-Label": "Confocal", "445 AO-Name": "AnalogIO", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-Timing-ReadoutTimeNs": "24672000.0000", "528 AO-Demo": "No", "520 AO-Sequenceable": "1", "555-Description": "DA device that is used as a shutter", "405-DA Device": "405 Shutter", "405 AO-TriggerSequenceLength": "1000", "640 AO-MinVolts": "0.0000", "640 Shutter-Block voltage": "false", "FrameIndex": 0, "PRIME-Port": "CMOS", "555 AO-MinVolts": "0.0000", "COM3-Description": "Serial port driver (boost:asio)", "528 AO-Description": "NI DAC", "555 AO-IOChannel": "Dev1/ao6", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "555 AO-TriggerInputLine": "/Dev1/PFI0", "PRIME-ActualInterval-ms": "84.6200", "LudlController-Port": "COM9", "445 Shutter-Demo": "No", "PRIME-Trigger-Expose Out-Mux": "1", "SliceIndex": 1, "PRIME-CCDTemperatureSetPoint": "-15.0000", "405 Shutter-MinVolts": "0.0000", "PRIME-ExposeOutMode": "First Row", "AxisPositions": null, "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Volts": "0.0000", "528-Name": "DA Shutter", "555-State": "0", "XLIGHT Dichroic-Label": "CFP/YFP", "445 AO-Sequenceable": "1", "405-Name": "DA Shutter", "405 AO-IOChannel": "Dev1/ao0", "Core-Camera": "PRIME", "Focus-Name": "Focus", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "405 Shutter-IOChannel": "Dev1/ao8", "640 AO-Demo": "No", "640 Shutter-SupportsTriggering": "0", "445 AO-Demo": "No", "555-DA Device": "555 Shutter", "470 Shutter-Description": "NI DAC", "528 AO-Volts": "0.0000", "COM1-DataBits": "8", "Vincent-D3-Address": "x", "520 AO-SupportsTriggering": "0", "405-Description": "DA device that is used as a shutter", "640 Shutter-MinVolts": "0.0000", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "405 AO-Name": "AnalogIO", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "640 AO-TriggerInputLine": "/Dev1/PFI0", "405 AO-TriggerInputLine": "/Dev1/PFI0", "640-Description": "DA device that is used as a shutter", "445 Shutter-TriggerSampleRate": "100000", "470 Shutter-TriggerSequenceLength": "1000", "COM1-StopBits": "1", "520 Shutter-SupportsTriggering": "0", "405-State": "0", "Core-Initialize": "1", "XLIGHT Emission Wheel-State": "3", "520 AO-Description": "NI DAC", "528 AO-TriggerInputLine": "/Dev1/PFI0", "445 Shutter-MaxVolts": "5.0000", "COM9-Handshaking": "Off", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "XLIGHT Spin motor-Label": "On (spinning)", "PRIME-PP 5 ENABLED": "No", "UUID": "a02821ea-45ad-46aa-949e-fe186dee7963", "PRIME-TriggerMode": "Internal Trigger", "COM1-Handshaking": "Off", "PRIME-Timing-PostTriggerDelayNs": "49344000.0000", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "Focus-Position": "16732.9000", "528 Shutter-Block voltage": "false", "Channel": "Il-G", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "555 Shutter-Demo": "No", "405 Shutter-Demo": "No", "528 AO-Name": "AnalogIO", "520-State": "1", "Core-Galvo": "", "405 Shutter-TriggerSampleRate": "100000", "Focus-FocusStepSize_(nm)": "100", "PRIME-PixelType": "16bit", "COM4-DataBits": "8", "470 AO-IOChannel": "Dev1/ao2", "520 AO-MinVolts": "0.0000", "COM9-Parity": "None", "PRIME-CircularBufferEnabled": "ON", "PRIME-PP 6 Reset": "No", "640 AO-Description": "NI DAC", "Source": "PRIME", "445 AO-MinVolts": "0.0000", "PIEZO-Name": "Stage", "445 AO-Description": "NI DAC", "PRIME-CircularBufferFrameCount": "46", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "520 AO-Volts": "4.5000", "528 Shutter-TriggerSampleRate": "100000", "XPositionUm": 23269.100000000002, "OpticalPath-State": "4", "PRIME-PP 3 THRESHOLD": "80", "PRIME-PVCAM Version": "3.7.1", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Gain": "1-HDR", "640 Shutter-TriggerSequenceLength": "1000", "528 AO-SupportsTriggering": "0", "445 AO-TriggerInputLine": "/Dev1/PFI0", "OpticalPath-Name": "OpticalPath", "PRIME-PP 4 ENABLED": "Yes", "PRIME-PVCAM Adapter Version": "1.3.29", "COM3-Name": "COM3", "405 AO-MaxVolts": "5.0000", "520 Shutter-Block voltage": "false", "PRIME-Timing-ClearingTimeNs": "49344000.0000", "Core-Focus": "PIEZO", "528 AO-Block voltage": "false", "TE2000-Description": "Nikon TE2000 hub", "528 AO-MinVolts": "0.0000", "COM4-Parity": "None", "Summary": { "Slices": 11, "ChContrastMin": [ 0, 0, 0, 0, 0, 0 ], "PixelAspect": 1, "SlicesFirst": true, "Interval_ms": 0, "Time": "2019-12-17 03:59:33 -0500", "Prefix": "seq_7", "InitialPositionList": [ { "Label": "Pos0", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [149.91], "Focus": [16657.7], "XYStage": [ 71629.7, 19152.9 ] }, "GridColumnIndex": 0 }, { "Label": "Pos1", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [190.82], "Focus": [16485.4], "XYStage": [ 65702.3, 19396.600000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos2", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [194.69], "Focus": [16446.8], "XYStage": [ 59880.5, 19007.8 ] }, "GridColumnIndex": 0 }, { "Label": "Pos3", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [145.04], "Focus": [16446.6], "XYStage": [ 53616.9, 18818.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos4", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [169.57500000000002], "Focus": [16446.5], "XYStage": [ 47662.8, 18817.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos5", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [131.805], "Focus": [16446.4], "XYStage": [ 41918.8, 18298.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos6", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [113.145], "Focus": [16446.5], "XYStage": [ 36129.9, 18895.8 ] }, "GridColumnIndex": 0 }, { "Label": "Pos7", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [141.005], "Focus": [16446.6], "XYStage": [ 30299.100000000002, 18895.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos8", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [140.97], "Focus": [16446.5], "XYStage": [ 23952.100000000002, 19156 ] }, "GridColumnIndex": 0 }, { "Label": "Pos9", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [184.57500000000002], "Focus": [16677.9], "XYStage": [ 72866.8, -14809.300000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos10", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [167.95000000000002], "Focus": [16557.9], "XYStage": [ 66515.6, -14371.300000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos11", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [168], "Focus": [16618.9], "XYStage": [ 59724.3, -13964.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos12", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [203.205], "Focus": [16779.6], "XYStage": [ 53862.3, -13494.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos13", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [203.20000000000002], "Focus": [16749], "XYStage": [ 48108.3, -13805.400000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos14", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [203.19], "Focus": [16675.9], "XYStage": [ 41645, -13804.900000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos15", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [203.21], "Focus": [16675.7], "XYStage": [ 35352.6, -13805.6 ] }, "GridColumnIndex": 0 }, { "Label": "Pos16", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [203.225], "Focus": [16675.8], "XYStage": [ 29298, -13447.400000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos17", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [203.215], "Focus": [16675.8], "XYStage": [ 23269.100000000002, -13012.300000000001 ] }, "GridColumnIndex": 0 } ], "z-step_um": 10, "GridRow": 0, "BitDepth": 16, "Depth": 2, "Channels": 6, "Source": "Micro-Manager", "ChColors": [ -16711681, -256, -65281, -1, -1, -16776961 ], "MetadataVersion": 10, "KeepShutterOpenChannels": true, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536, 65536 ], "CustomIntervals_ms": [], "Height": 1200, "MicroManagerVersion": "1.4.23 20180219", "GridColumn": 0, "UUID": "3ad8801b-5699-40d6-813b-4ad840863e38", "PixelType": "GRAY16", "Width": 1200, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\Yan\\20191216-YC113-S11S12-miseq", "Frames": 1, "Comment": "", "UserName": "Nikon", "PixelSize_um": 1.1, "ROI": [ 0, 0, 1200, 1200 ], "Date": "2019-12-17", "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC", "P-DAPI-high" ], "KeepShutterOpenSlices": true, "IJType": 1, "ComputerName": "ZadorConfocal2", "Positions": 18, "PositionIndex": 17, "CameraTimeout": 20000, "TimeFirst": false }, "640 AO-Name": "AnalogIO", "Vincent-D3-Command": "Close" }, "FrameKey-0-0-2": { "COM4-StopBits": "1", "528 AO-MaxVolts": "5.0000", "XLIGHT Excitation Wheel-State": "1", "470 Shutter-Name": "AnalogIO", "Core-Shutter": "520", "555 AO-Demo": "No", "XLIGHT Dichroic-State": "2", "XYStage-StepSize-Y": "0.1000", "ZPositionUm": 173.215, "XYStage-StepSize-X": "0.1000", "PRIME-FirmwareVersion": "20.28", "PRIME-ClearCycles": "2", "520-Name": "DA Shutter", "405 Shutter-Name": "AnalogIO", "PIEZO-StepSize": "0.0050", "PRIME-ClearMode": "Pre-Exposure", "470 AO-MinVolts": "0.0000", "405 AO-SupportsTriggering": "1", "CameraChannelIndex": 0, "XYStage-StartSpeed": "500.0000", "COM9-StopBits": "2", "405 AO-Description": "NI DAC", "COM4-DelayBetweenCharsMs": "0.0000", "445 AO-Block voltage": "false", "520 AO-TriggerInputLine": "/Dev1/PFI0", "555 AO-Description": "NI DAC", "520 AO-TriggerSampleRate": "100000", "COM4-Verbose": "1", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM4-Name": "COM4", "405 Shutter-Volts": "0.0000", "Vincent-D3-Port": "COM1", "PRIME-PP 4 MIN ADU AFFECTED": "200", "470 Shutter-MinVolts": "0.0000", "PRIME-PP 3 ENABLED": "Yes", "470 AO-Description": "NI DAC", "PRIME-Description": "PVCAM API device adapter", "520 Shutter-MaxVolts": "5.0000", "520 Shutter-Volts": "5.0000", "528 AO-Sequenceable": "1", "PRIME-Binning": "1x1", "555 Shutter-MaxVolts": "5.0000", "528 Shutter-TriggerSequenceLength": "1000", "405 Shutter-Block voltage": "false", "XLIGHT Spin motor-State": "1", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "Vincent-D3-Time to open (ms)": "35.0000", "Vincent-D3-Time to close (ms)": "35.0000", "445 AO-TriggerSampleRate": "100000", "640 Shutter-IOChannel": "Dev1/ao14", "555 AO-Sequenceable": "1", "520 AO-Name": "AnalogIO", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "470 AO-Block voltage": "false", "COM1-Verbose": "1", "COM9-AnswerTimeout": "10000.0000", "528 Shutter-Name": "AnalogIO", "PRIME-Trigger Timeout (secs)": "10", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "NextFrame": 0, "470 AO-TriggerSequenceLength": "1000", "PRIME-TransposeXY": "0", "445 AO-TriggerSequenceLength": "1000", "COM1-Name": "COM1", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "COM9-DataBits": "8", "COM1-AnswerTimeout": "500.0000", "Camera": "", "TE2000-Version": "V2.50-V2.50-V2.10", "528 Shutter-Volts": "0.0000", "XLIGHT Hub-Name": "XLIGHT Hub", "PIEZO-Description": "Ludl stage driver adapter", "PositionName": "Pos17", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSampleRate": "100000", "PRIME-FanSpeedSetpoint": "High", "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "405 AO-TriggerSampleRate": "100000", "470 Shutter-Block voltage": "false", "470-State": "0", "COM3-Parity": "None", "405 AO-Volts": "0.0000", "PRIME-TransposeMirrorY": "0", "PRIME-ChipName": "GS144BSI", "470 AO-Volts": "0.0000", "PRIME-TransposeMirrorX": "0", "445 Shutter-SupportsTriggering": "0", "470 Shutter-SupportsTriggering": "0", "XLIGHT Hub-Description": "XLIGHT hub", "PRIME-PreampOffLimit": "10000", "470 AO-Sequenceable": "1", "528 Shutter-MaxVolts": "5.0000", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "Core-XYStage": "XYStage", "470 AO-TriggerSampleRate": "100000", "PRIME-Timing-PreTriggerDelayNs": "24672000.0000", "528-DA Device": "528 Shutter", "Focus-Description": "Nikon TE2000 focus adapter", "FilterBlock-State": "5", "520 AO-Demo": "No", "COM3-DelayBetweenCharsMs": "0.0000", "470 Shutter-Volts": "0.0000", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "PRIME-PP 2 THRESHOLD": "125", "PRIME-PP 4 THRESHOLD": "75", "XYStage-Acceleration": "4.0000", "470 Shutter-Demo": "No", "PRIME-Name": "PMPCIECam00", "640-DA Device": "640 Shutter", "405 AO-Block voltage": "false", "ElapsedTime-ms": 717396, "PRIME-Offset": "100", "445 AO-IOChannel": "Dev1/ao1", "640 AO-MaxVolts": "5.0000", "PositionIndex": 17, "PRIME-PP 1 ENABLED": "Yes", "COM1-BaudRate": "9600", "640-State": "0", "COM3-Verbose": "1", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "LudlController-TransmissionDelay": "0", "PRIME-FullWellCapacity": "62000", "PRIME-SMARTStreamingEnabled": "No", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "640 Shutter-Description": "NI DAC", "528-Description": "DA device that is used as a shutter", "COM4-Description": "Serial port driver (boost:asio)", "405 Shutter-Description": "NI DAC", "TE2000-Name": "TE2000", "528 Shutter-Description": "NI DAC", "COM9-Description": "Serial port driver (boost:asio)", "LudlController-Name": "LudlController", "445 Shutter-TriggerSequenceLength": "1000", "PRIME-SerialNumber": "A18B203008", "445 AO-Volts": "0.0000", "PRIME-X-dimension": "1200", "PRIME-ShutterCloseDelay": "0", "XLIGHT Disk slider-State": "2", "Position": "Default", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 AO-IOChannel": "Dev1/ao5", "520 AO-IOChannel": "Dev1/ao4", "528 AO-TriggerSequenceLength": "1000", "405 Shutter-MaxVolts": "5.0000", "470-DA Device": "470 Shutter", "PRIME-FTCapable": "No", "Core-ChannelGroup": "Channels", "555 AO-SupportsTriggering": "0", "PRIME-Y-dimension": "1200", "Frame": 0, "555 Shutter-Sequenceable": "1", "PRIME-Timing-ExposureTimeNs": "499998000.0000", "Core-ImageProcessor": "", "528-State": "0", "520 AO-Block voltage": "false", "XLIGHT Excitation Wheel-Label": "CFP/YFP", "640 Shutter-Demo": "No", "COM3-StopBits": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "520 Shutter-IOChannel": "Dev1/ao11", "Width": 1200, "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-SupportsTriggering": "0", "640 AO-TriggerSampleRate": "100000", "520-Description": "DA device that is used as a shutter", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "405 Shutter-Sequenceable": "1", "405 Shutter-SupportsTriggering": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-TriggerSampleRate": "100000", "640 Shutter-MaxVolts": "5.0000", "555 AO-MaxVolts": "5.0000", "520 AO-TriggerSequenceLength": "1000", "COM3-BaudRate": "9600", "COM9-BaudRate": "9600", "Vincent-D3-Channel #": "Ch. #1", "PRIME-PMode": "Normal", "OpticalPath-Label": "Left(100)", "BitDepth": 16, "555 AO-TriggerSequenceLength": "1000", "445 Shutter-Description": "NI DAC", "405 AO-Sequenceable": "1", "PRIME-AcquisitionMethod": "Callbacks", "555 AO-Name": "AnalogIO", "PRIME-ShutterMode": "Pre-Exposure", "COM3-Handshaking": "Off", "445 AO-SupportsTriggering": "0", "ROI": "0-0-1200-1200", "COM9-Verbose": "1", "PRIME-Color": "OFF", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "PRIME-ShutterOpenDelay": "0", "COM1-Parity": "None", "Slice": 2, "SlicePosition": -30, "COM9-DelayBetweenCharsMs": "0.0000", "555-Name": "DA Shutter", "PIEZO-LudlSingleAxisName": "B", "XLIGHT Emission Wheel-Label": "565/24(G)", "640 Shutter-Name": "AnalogIO", "640 AO-Sequenceable": "1", "520 Shutter-Demo": "No", "528 Shutter-Demo": "No", "470-Name": "DA Shutter", "520-DA Device": "520 Shutter", "XYStage-Description": "Ludl XY stage driver adapter", "445 Shutter-MinVolts": "0.0000", "528 AO-TriggerSampleRate": "100000", "405 AO-MinVolts": "0.0000", "405 Shutter-TriggerSequenceLength": "1000", "Height": 1200, "555 AO-Block voltage": "false", "640 Shutter-Volts": "0.0000", "PixelType": "GRAY16", "445 Shutter-Block voltage": "false", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "520 Shutter-TriggerSequenceLength": "1000", "COM9-Name": "COM9", "Core-SLM": "", "555 AO-Volts": "0.0000", "555 Shutter-Block voltage": "false", "XYStage-Speed": "1800.0000", "520 AO-MaxVolts": "5.0000", "528 Shutter-SupportsTriggering": "0", "COM1-Description": "Serial port driver (boost:asio)", "PRIME-MetadataEnabled": "No", "520 Shutter-MinVolts": "0.0000", "470 Shutter-Sequenceable": "1", "PRIME-PreampDelay": "0", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-MinVolts": "0.0000", "ChannelIndex": 0, "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Core-AutoShutter": "1", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "Exposure-ms": 320, "PRIME-PP 2 ENABLED": "Yes", "640 Shutter-TriggerSampleRate": "100000", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "470 Shutter-IOChannel": "Dev1/ao10", "405 AO-Demo": "No", "PixelSizeUm": 1.1, "470 AO-Demo": "No", "470 Shutter-MaxVolts": "5.0000", "Vincent-D3-Name": "Vincent-D3", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FileName": "img_000000000_Il-G_002.tif", "LudlController-Reset": "Operate", "PRIME-Exposure": "320.00", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "445-Name": "DA Shutter", "555 Shutter-Name": "AnalogIO", "520 Shutter-TriggerSampleRate": "100000", "445 Shutter-IOChannel": "Dev1/ao9", "PRIME-TransposeCorrection": "0", "XYStage-StepSize": "0.1000", "445-Description": "DA device that is used as a shutter", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 AO-IOChannel": "Dev1/ao7", "520 Shutter-Name": "AnalogIO", "COM3-DataBits": "8", "640 AO-Volts": "0.0000", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "555 Shutter-IOChannel": "Dev1/ao13", "WaitInterval": null, "520 Shutter-Sequenceable": "1", "YPositionUm": -13012.300000000001, "445-State": "0", "445-DA Device": "445 Shutter", "PRIME-CircularBufferAutoSize": "ON", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "555 AO-TriggerSampleRate": "100000", "445 Shutter-Volts": "0.0000", "XLIGHT Hub-Port": "COM3", "Core-AutoFocus": "", "COM4-Handshaking": "Off", "528 Shutter-MinVolts": "0.0000", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "445 Shutter-Name": "AnalogIO", "528 Shutter-Sequenceable": "1", "XYStage-TransposeMirrorX": "0", "XYStage-TransposeMirrorY": "0", "PRIME-ReadoutRate": "100MHz 16bit", "XYStage-Name": "Stage", "640 AO-SupportsTriggering": "0", "640-Name": "DA Shutter", "640 Shutter-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "640 AO-Block voltage": "false", "445 Shutter-Sequenceable": "1", "470 AO-MaxVolts": "5.0000", "COM4-BaudRate": "9600", "470-Description": "DA device that is used as a shutter", "520 Shutter-Description": "NI DAC", "PRIME-CCDTemperature": "-14.9200", "Time": "2019-12-17 03:59:34 -0500", "640 AO-TriggerSequenceLength": "1000", "470 AO-Name": "AnalogIO", "XLIGHT Disk slider-Label": "Disk Pos 40um", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "Binning": "1x1", "TE2000-ModelType": "1", "COM3-AnswerTimeout": "500.0000", "555 Shutter-SupportsTriggering": "0", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "TE2000-Port": "COM4", "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Description": "NI DAC", "COM4-AnswerTimeout": "500.0000", "COM1-DelayBetweenCharsMs": "0.0000", "FilterBlock-Label": "Confocal", "445 AO-Name": "AnalogIO", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-Timing-ReadoutTimeNs": "24672000.0000", "528 AO-Demo": "No", "520 AO-Sequenceable": "1", "555-Description": "DA device that is used as a shutter", "405-DA Device": "405 Shutter", "405 AO-TriggerSequenceLength": "1000", "640 AO-MinVolts": "0.0000", "640 Shutter-Block voltage": "false", "FrameIndex": 0, "PRIME-Port": "CMOS", "555 AO-MinVolts": "0.0000", "COM3-Description": "Serial port driver (boost:asio)", "528 AO-Description": "NI DAC", "555 AO-IOChannel": "Dev1/ao6", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "555 AO-TriggerInputLine": "/Dev1/PFI0", "PRIME-ActualInterval-ms": "84.6200", "LudlController-Port": "COM9", "445 Shutter-Demo": "No", "PRIME-Trigger-Expose Out-Mux": "1", "SliceIndex": 2, "PRIME-CCDTemperatureSetPoint": "-15.0000", "405 Shutter-MinVolts": "0.0000", "PRIME-ExposeOutMode": "First Row", "AxisPositions": null, "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Volts": "0.0000", "528-Name": "DA Shutter", "555-State": "0", "XLIGHT Dichroic-Label": "CFP/YFP", "445 AO-Sequenceable": "1", "405-Name": "DA Shutter", "405 AO-IOChannel": "Dev1/ao0", "Core-Camera": "PRIME", "Focus-Name": "Focus", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "405 Shutter-IOChannel": "Dev1/ao8", "640 AO-Demo": "No", "640 Shutter-SupportsTriggering": "0", "445 AO-Demo": "No", "555-DA Device": "555 Shutter", "470 Shutter-Description": "NI DAC", "528 AO-Volts": "0.0000", "COM1-DataBits": "8", "Vincent-D3-Address": "x", "520 AO-SupportsTriggering": "0", "405-Description": "DA device that is used as a shutter", "640 Shutter-MinVolts": "0.0000", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "405 AO-Name": "AnalogIO", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "640 AO-TriggerInputLine": "/Dev1/PFI0", "405 AO-TriggerInputLine": "/Dev1/PFI0", "640-Description": "DA device that is used as a shutter", "445 Shutter-TriggerSampleRate": "100000", "470 Shutter-TriggerSequenceLength": "1000", "COM1-StopBits": "1", "520 Shutter-SupportsTriggering": "0", "405-State": "0", "Core-Initialize": "1", "XLIGHT Emission Wheel-State": "3", "520 AO-Description": "NI DAC", "528 AO-TriggerInputLine": "/Dev1/PFI0", "445 Shutter-MaxVolts": "5.0000", "COM9-Handshaking": "Off", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "XLIGHT Spin motor-Label": "On (spinning)", "PRIME-PP 5 ENABLED": "No", "UUID": "ef2ec159-3578-4b02-abc4-31505fa202e0", "PRIME-TriggerMode": "Internal Trigger", "COM1-Handshaking": "Off", "PRIME-Timing-PostTriggerDelayNs": "49344000.0000", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "Focus-Position": "16732.9000", "528 Shutter-Block voltage": "false", "Channel": "Il-G", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "555 Shutter-Demo": "No", "405 Shutter-Demo": "No", "528 AO-Name": "AnalogIO", "520-State": "1", "Core-Galvo": "", "405 Shutter-TriggerSampleRate": "100000", "Focus-FocusStepSize_(nm)": "100", "PRIME-PixelType": "16bit", "COM4-DataBits": "8", "470 AO-IOChannel": "Dev1/ao2", "520 AO-MinVolts": "0.0000", "COM9-Parity": "None", "PRIME-CircularBufferEnabled": "ON", "PRIME-PP 6 Reset": "No", "640 AO-Description": "NI DAC", "Source": "PRIME", "445 AO-MinVolts": "0.0000", "PIEZO-Name": "Stage", "445 AO-Description": "NI DAC", "PRIME-CircularBufferFrameCount": "46", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "520 AO-Volts": "4.5000", "528 Shutter-TriggerSampleRate": "100000", "XPositionUm": 23269.100000000002, "OpticalPath-State": "4", "PRIME-PP 3 THRESHOLD": "80", "PRIME-PVCAM Version": "3.7.1", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Gain": "1-HDR", "640 Shutter-TriggerSequenceLength": "1000", "528 AO-SupportsTriggering": "0", "445 AO-TriggerInputLine": "/Dev1/PFI0", "OpticalPath-Name": "OpticalPath", "PRIME-PP 4 ENABLED": "Yes", "PRIME-PVCAM Adapter Version": "1.3.29", "COM3-Name": "COM3", "405 AO-MaxVolts": "5.0000", "520 Shutter-Block voltage": "false", "PRIME-Timing-ClearingTimeNs": "49344000.0000", "Core-Focus": "PIEZO", "528 AO-Block voltage": "false", "TE2000-Description": "Nikon TE2000 hub", "528 AO-MinVolts": "0.0000", "COM4-Parity": "None", "Summary": { "Slices": 11, "ChContrastMin": [ 0, 0, 0, 0, 0, 0 ], "PixelAspect": 1, "SlicesFirst": true, "Interval_ms": 0, "Time": "2019-12-17 03:59:33 -0500", "Prefix": "seq_7", "InitialPositionList": [ { "Label": "Pos0", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [149.91], "Focus": [16657.7], "XYStage": [ 71629.7, 19152.9 ] }, "GridColumnIndex": 0 }, { "Label": "Pos1", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [190.82], "Focus": [16485.4], "XYStage": [ 65702.3, 19396.600000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos2", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [194.69], "Focus": [16446.8], "XYStage": [ 59880.5, 19007.8 ] }, "GridColumnIndex": 0 }, { "Label": "Pos3", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [145.04], "Focus": [16446.6], "XYStage": [ 53616.9, 18818.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos4", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [169.57500000000002], "Focus": [16446.5], "XYStage": [ 47662.8, 18817.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos5", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [131.805], "Focus": [16446.4], "XYStage": [ 41918.8, 18298.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos6", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [113.145], "Focus": [16446.5], "XYStage": [ 36129.9, 18895.8 ] }, "GridColumnIndex": 0 }, { "Label": "Pos7", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [141.005], "Focus": [16446.6], "XYStage": [ 30299.100000000002, 18895.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos8", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [140.97], "Focus": [16446.5], "XYStage": [ 23952.100000000002, 19156 ] }, "GridColumnIndex": 0 }, { "Label": "Pos9", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [184.57500000000002], "Focus": [16677.9], "XYStage": [ 72866.8, -14809.300000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos10", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [167.95000000000002], "Focus": [16557.9], "XYStage": [ 66515.6, -14371.300000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos11", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [168], "Focus": [16618.9], "XYStage": [ 59724.3, -13964.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos12", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [203.205], "Focus": [16779.6], "XYStage": [ 53862.3, -13494.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos13", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [203.20000000000002], "Focus": [16749], "XYStage": [ 48108.3, -13805.400000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos14", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [203.19], "Focus": [16675.9], "XYStage": [ 41645, -13804.900000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos15", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [203.21], "Focus": [16675.7], "XYStage": [ 35352.6, -13805.6 ] }, "GridColumnIndex": 0 }, { "Label": "Pos16", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [203.225], "Focus": [16675.8], "XYStage": [ 29298, -13447.400000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos17", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [203.215], "Focus": [16675.8], "XYStage": [ 23269.100000000002, -13012.300000000001 ] }, "GridColumnIndex": 0 } ], "z-step_um": 10, "GridRow": 0, "BitDepth": 16, "Depth": 2, "Channels": 6, "Source": "Micro-Manager", "ChColors": [ -16711681, -256, -65281, -1, -1, -16776961 ], "MetadataVersion": 10, "KeepShutterOpenChannels": true, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536, 65536 ], "CustomIntervals_ms": [], "Height": 1200, "MicroManagerVersion": "1.4.23 20180219", "GridColumn": 0, "UUID": "3ad8801b-5699-40d6-813b-4ad840863e38", "PixelType": "GRAY16", "Width": 1200, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\Yan\\20191216-YC113-S11S12-miseq", "Frames": 1, "Comment": "", "UserName": "Nikon", "PixelSize_um": 1.1, "ROI": [ 0, 0, 1200, 1200 ], "Date": "2019-12-17", "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC", "P-DAPI-high" ], "KeepShutterOpenSlices": true, "IJType": 1, "ComputerName": "ZadorConfocal2", "Positions": 18, "PositionIndex": 17, "CameraTimeout": 20000, "TimeFirst": false }, "640 AO-Name": "AnalogIO", "Vincent-D3-Command": "Close" }, "FrameKey-0-0-3": { "COM4-StopBits": "1", "528 AO-MaxVolts": "5.0000", "XLIGHT Excitation Wheel-State": "1", "470 Shutter-Name": "AnalogIO", "Core-Shutter": "520", "555 AO-Demo": "No", "XLIGHT Dichroic-State": "2", "XYStage-StepSize-Y": "0.1000", "ZPositionUm": 183.215, "XYStage-StepSize-X": "0.1000", "PRIME-FirmwareVersion": "20.28", "PRIME-ClearCycles": "2", "520-Name": "DA Shutter", "405 Shutter-Name": "AnalogIO", "PIEZO-StepSize": "0.0050", "PRIME-ClearMode": "Pre-Exposure", "470 AO-MinVolts": "0.0000", "405 AO-SupportsTriggering": "1", "CameraChannelIndex": 0, "XYStage-StartSpeed": "500.0000", "COM9-StopBits": "2", "405 AO-Description": "NI DAC", "COM4-DelayBetweenCharsMs": "0.0000", "445 AO-Block voltage": "false", "520 AO-TriggerInputLine": "/Dev1/PFI0", "555 AO-Description": "NI DAC", "520 AO-TriggerSampleRate": "100000", "COM4-Verbose": "1", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM4-Name": "COM4", "405 Shutter-Volts": "0.0000", "Vincent-D3-Port": "COM1", "PRIME-PP 4 MIN ADU AFFECTED": "200", "470 Shutter-MinVolts": "0.0000", "PRIME-PP 3 ENABLED": "Yes", "470 AO-Description": "NI DAC", "PRIME-Description": "PVCAM API device adapter", "520 Shutter-MaxVolts": "5.0000", "520 Shutter-Volts": "5.0000", "528 AO-Sequenceable": "1", "PRIME-Binning": "1x1", "555 Shutter-MaxVolts": "5.0000", "528 Shutter-TriggerSequenceLength": "1000", "405 Shutter-Block voltage": "false", "XLIGHT Spin motor-State": "1", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "Vincent-D3-Time to open (ms)": "35.0000", "Vincent-D3-Time to close (ms)": "35.0000", "445 AO-TriggerSampleRate": "100000", "640 Shutter-IOChannel": "Dev1/ao14", "555 AO-Sequenceable": "1", "520 AO-Name": "AnalogIO", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "470 AO-Block voltage": "false", "COM1-Verbose": "1", "COM9-AnswerTimeout": "10000.0000", "528 Shutter-Name": "AnalogIO", "PRIME-Trigger Timeout (secs)": "10", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "NextFrame": 0, "470 AO-TriggerSequenceLength": "1000", "PRIME-TransposeXY": "0", "445 AO-TriggerSequenceLength": "1000", "COM1-Name": "COM1", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "COM9-DataBits": "8", "COM1-AnswerTimeout": "500.0000", "Camera": "", "TE2000-Version": "V2.50-V2.50-V2.10", "528 Shutter-Volts": "0.0000", "XLIGHT Hub-Name": "XLIGHT Hub", "PIEZO-Description": "Ludl stage driver adapter", "PositionName": "Pos17", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSampleRate": "100000", "PRIME-FanSpeedSetpoint": "High", "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "405 AO-TriggerSampleRate": "100000", "470 Shutter-Block voltage": "false", "470-State": "0", "COM3-Parity": "None", "405 AO-Volts": "0.0000", "PRIME-TransposeMirrorY": "0", "PRIME-ChipName": "GS144BSI", "470 AO-Volts": "0.0000", "PRIME-TransposeMirrorX": "0", "445 Shutter-SupportsTriggering": "0", "470 Shutter-SupportsTriggering": "0", "XLIGHT Hub-Description": "XLIGHT hub", "PRIME-PreampOffLimit": "10000", "470 AO-Sequenceable": "1", "528 Shutter-MaxVolts": "5.0000", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "Core-XYStage": "XYStage", "470 AO-TriggerSampleRate": "100000", "PRIME-Timing-PreTriggerDelayNs": "24672000.0000", "528-DA Device": "528 Shutter", "Focus-Description": "Nikon TE2000 focus adapter", "FilterBlock-State": "5", "520 AO-Demo": "No", "COM3-DelayBetweenCharsMs": "0.0000", "470 Shutter-Volts": "0.0000", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "PRIME-PP 2 THRESHOLD": "125", "PRIME-PP 4 THRESHOLD": "75", "XYStage-Acceleration": "4.0000", "470 Shutter-Demo": "No", "PRIME-Name": "PMPCIECam00", "640-DA Device": "640 Shutter", "405 AO-Block voltage": "false", "ElapsedTime-ms": 717952, "PRIME-Offset": "100", "445 AO-IOChannel": "Dev1/ao1", "640 AO-MaxVolts": "5.0000", "PositionIndex": 17, "PRIME-PP 1 ENABLED": "Yes", "COM1-BaudRate": "9600", "640-State": "0", "COM3-Verbose": "1", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "LudlController-TransmissionDelay": "0", "PRIME-FullWellCapacity": "62000", "PRIME-SMARTStreamingEnabled": "No", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "640 Shutter-Description": "NI DAC", "528-Description": "DA device that is used as a shutter", "COM4-Description": "Serial port driver (boost:asio)", "405 Shutter-Description": "NI DAC", "TE2000-Name": "TE2000", "528 Shutter-Description": "NI DAC", "COM9-Description": "Serial port driver (boost:asio)", "LudlController-Name": "LudlController", "445 Shutter-TriggerSequenceLength": "1000", "PRIME-SerialNumber": "A18B203008", "445 AO-Volts": "0.0000", "PRIME-X-dimension": "1200", "PRIME-ShutterCloseDelay": "0", "XLIGHT Disk slider-State": "2", "Position": "Default", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 AO-IOChannel": "Dev1/ao5", "520 AO-IOChannel": "Dev1/ao4", "528 AO-TriggerSequenceLength": "1000", "405 Shutter-MaxVolts": "5.0000", "470-DA Device": "470 Shutter", "PRIME-FTCapable": "No", "Core-ChannelGroup": "Channels", "555 AO-SupportsTriggering": "0", "PRIME-Y-dimension": "1200", "Frame": 0, "555 Shutter-Sequenceable": "1", "PRIME-Timing-ExposureTimeNs": "499998000.0000", "Core-ImageProcessor": "", "528-State": "0", "520 AO-Block voltage": "false", "XLIGHT Excitation Wheel-Label": "CFP/YFP", "640 Shutter-Demo": "No", "COM3-StopBits": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "520 Shutter-IOChannel": "Dev1/ao11", "Width": 1200, "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-SupportsTriggering": "0", "640 AO-TriggerSampleRate": "100000", "520-Description": "DA device that is used as a shutter", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "405 Shutter-Sequenceable": "1", "405 Shutter-SupportsTriggering": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-TriggerSampleRate": "100000", "640 Shutter-MaxVolts": "5.0000", "555 AO-MaxVolts": "5.0000", "520 AO-TriggerSequenceLength": "1000", "COM3-BaudRate": "9600", "COM9-BaudRate": "9600", "Vincent-D3-Channel #": "Ch. #1", "PRIME-PMode": "Normal", "OpticalPath-Label": "Left(100)", "BitDepth": 16, "555 AO-TriggerSequenceLength": "1000", "445 Shutter-Description": "NI DAC", "405 AO-Sequenceable": "1", "PRIME-AcquisitionMethod": "Callbacks", "555 AO-Name": "AnalogIO", "PRIME-ShutterMode": "Pre-Exposure", "COM3-Handshaking": "Off", "445 AO-SupportsTriggering": "0", "ROI": "0-0-1200-1200", "COM9-Verbose": "1", "PRIME-Color": "OFF", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "PRIME-ShutterOpenDelay": "0", "COM1-Parity": "None", "Slice": 3, "SlicePosition": -20, "COM9-DelayBetweenCharsMs": "0.0000", "555-Name": "DA Shutter", "PIEZO-LudlSingleAxisName": "B", "XLIGHT Emission Wheel-Label": "565/24(G)", "640 Shutter-Name": "AnalogIO", "640 AO-Sequenceable": "1", "520 Shutter-Demo": "No", "528 Shutter-Demo": "No", "470-Name": "DA Shutter", "520-DA Device": "520 Shutter", "XYStage-Description": "Ludl XY stage driver adapter", "445 Shutter-MinVolts": "0.0000", "528 AO-TriggerSampleRate": "100000", "405 AO-MinVolts": "0.0000", "405 Shutter-TriggerSequenceLength": "1000", "Height": 1200, "555 AO-Block voltage": "false", "640 Shutter-Volts": "0.0000", "PixelType": "GRAY16", "445 Shutter-Block voltage": "false", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "520 Shutter-TriggerSequenceLength": "1000", "COM9-Name": "COM9", "Core-SLM": "", "555 AO-Volts": "0.0000", "555 Shutter-Block voltage": "false", "XYStage-Speed": "1800.0000", "520 AO-MaxVolts": "5.0000", "528 Shutter-SupportsTriggering": "0", "COM1-Description": "Serial port driver (boost:asio)", "PRIME-MetadataEnabled": "No", "520 Shutter-MinVolts": "0.0000", "470 Shutter-Sequenceable": "1", "PRIME-PreampDelay": "0", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-MinVolts": "0.0000", "ChannelIndex": 0, "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Core-AutoShutter": "1", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "Exposure-ms": 320, "PRIME-PP 2 ENABLED": "Yes", "640 Shutter-TriggerSampleRate": "100000", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "470 Shutter-IOChannel": "Dev1/ao10", "405 AO-Demo": "No", "PixelSizeUm": 1.1, "470 AO-Demo": "No", "470 Shutter-MaxVolts": "5.0000", "Vincent-D3-Name": "Vincent-D3", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FileName": "img_000000000_Il-G_003.tif", "LudlController-Reset": "Operate", "PRIME-Exposure": "320.00", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "445-Name": "DA Shutter", "555 Shutter-Name": "AnalogIO", "520 Shutter-TriggerSampleRate": "100000", "445 Shutter-IOChannel": "Dev1/ao9", "PRIME-TransposeCorrection": "0", "XYStage-StepSize": "0.1000", "445-Description": "DA device that is used as a shutter", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 AO-IOChannel": "Dev1/ao7", "520 Shutter-Name": "AnalogIO", "COM3-DataBits": "8", "640 AO-Volts": "0.0000", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "555 Shutter-IOChannel": "Dev1/ao13", "WaitInterval": null, "520 Shutter-Sequenceable": "1", "YPositionUm": -13012.300000000001, "445-State": "0", "445-DA Device": "445 Shutter", "PRIME-CircularBufferAutoSize": "ON", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "555 AO-TriggerSampleRate": "100000", "445 Shutter-Volts": "0.0000", "XLIGHT Hub-Port": "COM3", "Core-AutoFocus": "", "COM4-Handshaking": "Off", "528 Shutter-MinVolts": "0.0000", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "445 Shutter-Name": "AnalogIO", "528 Shutter-Sequenceable": "1", "XYStage-TransposeMirrorX": "0", "XYStage-TransposeMirrorY": "0", "PRIME-ReadoutRate": "100MHz 16bit", "XYStage-Name": "Stage", "640 AO-SupportsTriggering": "0", "640-Name": "DA Shutter", "640 Shutter-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "640 AO-Block voltage": "false", "445 Shutter-Sequenceable": "1", "470 AO-MaxVolts": "5.0000", "COM4-BaudRate": "9600", "470-Description": "DA device that is used as a shutter", "520 Shutter-Description": "NI DAC", "PRIME-CCDTemperature": "-14.9200", "Time": "2019-12-17 03:59:34 -0500", "640 AO-TriggerSequenceLength": "1000", "470 AO-Name": "AnalogIO", "XLIGHT Disk slider-Label": "Disk Pos 40um", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "Binning": "1x1", "TE2000-ModelType": "1", "COM3-AnswerTimeout": "500.0000", "555 Shutter-SupportsTriggering": "0", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "TE2000-Port": "COM4", "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Description": "NI DAC", "COM4-AnswerTimeout": "500.0000", "COM1-DelayBetweenCharsMs": "0.0000", "FilterBlock-Label": "Confocal", "445 AO-Name": "AnalogIO", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-Timing-ReadoutTimeNs": "24672000.0000", "528 AO-Demo": "No", "520 AO-Sequenceable": "1", "555-Description": "DA device that is used as a shutter", "405-DA Device": "405 Shutter", "405 AO-TriggerSequenceLength": "1000", "640 AO-MinVolts": "0.0000", "640 Shutter-Block voltage": "false", "FrameIndex": 0, "PRIME-Port": "CMOS", "555 AO-MinVolts": "0.0000", "COM3-Description": "Serial port driver (boost:asio)", "528 AO-Description": "NI DAC", "555 AO-IOChannel": "Dev1/ao6", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "555 AO-TriggerInputLine": "/Dev1/PFI0", "PRIME-ActualInterval-ms": "84.6200", "LudlController-Port": "COM9", "445 Shutter-Demo": "No", "PRIME-Trigger-Expose Out-Mux": "1", "SliceIndex": 3, "PRIME-CCDTemperatureSetPoint": "-15.0000", "405 Shutter-MinVolts": "0.0000", "PRIME-ExposeOutMode": "First Row", "AxisPositions": null, "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Volts": "0.0000", "528-Name": "DA Shutter", "555-State": "0", "XLIGHT Dichroic-Label": "CFP/YFP", "445 AO-Sequenceable": "1", "405-Name": "DA Shutter", "405 AO-IOChannel": "Dev1/ao0", "Core-Camera": "PRIME", "Focus-Name": "Focus", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "405 Shutter-IOChannel": "Dev1/ao8", "640 AO-Demo": "No", "640 Shutter-SupportsTriggering": "0", "445 AO-Demo": "No", "555-DA Device": "555 Shutter", "470 Shutter-Description": "NI DAC", "528 AO-Volts": "0.0000", "COM1-DataBits": "8", "Vincent-D3-Address": "x", "520 AO-SupportsTriggering": "0", "405-Description": "DA device that is used as a shutter", "640 Shutter-MinVolts": "0.0000", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "405 AO-Name": "AnalogIO", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "640 AO-TriggerInputLine": "/Dev1/PFI0", "405 AO-TriggerInputLine": "/Dev1/PFI0", "640-Description": "DA device that is used as a shutter", "445 Shutter-TriggerSampleRate": "100000", "470 Shutter-TriggerSequenceLength": "1000", "COM1-StopBits": "1", "520 Shutter-SupportsTriggering": "0", "405-State": "0", "Core-Initialize": "1", "XLIGHT Emission Wheel-State": "3", "520 AO-Description": "NI DAC", "528 AO-TriggerInputLine": "/Dev1/PFI0", "445 Shutter-MaxVolts": "5.0000", "COM9-Handshaking": "Off", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "XLIGHT Spin motor-Label": "On (spinning)", "PRIME-PP 5 ENABLED": "No", "UUID": "0767c835-20f7-4a33-ac16-9035922da46f", "PRIME-TriggerMode": "Internal Trigger", "COM1-Handshaking": "Off", "PRIME-Timing-PostTriggerDelayNs": "49344000.0000", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "Focus-Position": "16732.9000", "528 Shutter-Block voltage": "false", "Channel": "Il-G", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "555 Shutter-Demo": "No", "405 Shutter-Demo": "No", "528 AO-Name": "AnalogIO", "520-State": "1", "Core-Galvo": "", "405 Shutter-TriggerSampleRate": "100000", "Focus-FocusStepSize_(nm)": "100", "PRIME-PixelType": "16bit", "COM4-DataBits": "8", "470 AO-IOChannel": "Dev1/ao2", "520 AO-MinVolts": "0.0000", "COM9-Parity": "None", "PRIME-CircularBufferEnabled": "ON", "PRIME-PP 6 Reset": "No", "640 AO-Description": "NI DAC", "Source": "PRIME", "445 AO-MinVolts": "0.0000", "PIEZO-Name": "Stage", "445 AO-Description": "NI DAC", "PRIME-CircularBufferFrameCount": "46", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "520 AO-Volts": "4.5000", "528 Shutter-TriggerSampleRate": "100000", "XPositionUm": 23269.100000000002, "OpticalPath-State": "4", "PRIME-PP 3 THRESHOLD": "80", "PRIME-PVCAM Version": "3.7.1", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Gain": "1-HDR", "640 Shutter-TriggerSequenceLength": "1000", "528 AO-SupportsTriggering": "0", "445 AO-TriggerInputLine": "/Dev1/PFI0", "OpticalPath-Name": "OpticalPath", "PRIME-PP 4 ENABLED": "Yes", "PRIME-PVCAM Adapter Version": "1.3.29", "COM3-Name": "COM3", "405 AO-MaxVolts": "5.0000", "520 Shutter-Block voltage": "false", "PRIME-Timing-ClearingTimeNs": "49344000.0000", "Core-Focus": "PIEZO", "528 AO-Block voltage": "false", "TE2000-Description": "Nikon TE2000 hub", "528 AO-MinVolts": "0.0000", "COM4-Parity": "None", "Summary": { "Slices": 11, "ChContrastMin": [ 0, 0, 0, 0, 0, 0 ], "PixelAspect": 1, "SlicesFirst": true, "Interval_ms": 0, "Time": "2019-12-17 03:59:33 -0500", "Prefix": "seq_7", "InitialPositionList": [ { "Label": "Pos0", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [149.91], "Focus": [16657.7], "XYStage": [ 71629.7, 19152.9 ] }, "GridColumnIndex": 0 }, { "Label": "Pos1", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [190.82], "Focus": [16485.4], "XYStage": [ 65702.3, 19396.600000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos2", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [194.69], "Focus": [16446.8], "XYStage": [ 59880.5, 19007.8 ] }, "GridColumnIndex": 0 }, { "Label": "Pos3", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [145.04], "Focus": [16446.6], "XYStage": [ 53616.9, 18818.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos4", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [169.57500000000002], "Focus": [16446.5], "XYStage": [ 47662.8, 18817.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos5", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [131.805], "Focus": [16446.4], "XYStage": [ 41918.8, 18298.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos6", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [113.145], "Focus": [16446.5], "XYStage": [ 36129.9, 18895.8 ] }, "GridColumnIndex": 0 }, { "Label": "Pos7", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [141.005], "Focus": [16446.6], "XYStage": [ 30299.100000000002, 18895.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos8", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [140.97], "Focus": [16446.5], "XYStage": [ 23952.100000000002, 19156 ] }, "GridColumnIndex": 0 }, { "Label": "Pos9", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [184.57500000000002], "Focus": [16677.9], "XYStage": [ 72866.8, -14809.300000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos10", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [167.95000000000002], "Focus": [16557.9], "XYStage": [ 66515.6, -14371.300000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos11", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [168], "Focus": [16618.9], "XYStage": [ 59724.3, -13964.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos12", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [203.205], "Focus": [16779.6], "XYStage": [ 53862.3, -13494.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos13", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [203.20000000000002], "Focus": [16749], "XYStage": [ 48108.3, -13805.400000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos14", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [203.19], "Focus": [16675.9], "XYStage": [ 41645, -13804.900000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos15", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [203.21], "Focus": [16675.7], "XYStage": [ 35352.6, -13805.6 ] }, "GridColumnIndex": 0 }, { "Label": "Pos16", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [203.225], "Focus": [16675.8], "XYStage": [ 29298, -13447.400000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos17", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [203.215], "Focus": [16675.8], "XYStage": [ 23269.100000000002, -13012.300000000001 ] }, "GridColumnIndex": 0 } ], "z-step_um": 10, "GridRow": 0, "BitDepth": 16, "Depth": 2, "Channels": 6, "Source": "Micro-Manager", "ChColors": [ -16711681, -256, -65281, -1, -1, -16776961 ], "MetadataVersion": 10, "KeepShutterOpenChannels": true, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536, 65536 ], "CustomIntervals_ms": [], "Height": 1200, "MicroManagerVersion": "1.4.23 20180219", "GridColumn": 0, "UUID": "3ad8801b-5699-40d6-813b-4ad840863e38", "PixelType": "GRAY16", "Width": 1200, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\Yan\\20191216-YC113-S11S12-miseq", "Frames": 1, "Comment": "", "UserName": "Nikon", "PixelSize_um": 1.1, "ROI": [ 0, 0, 1200, 1200 ], "Date": "2019-12-17", "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC", "P-DAPI-high" ], "KeepShutterOpenSlices": true, "IJType": 1, "ComputerName": "ZadorConfocal2", "Positions": 18, "PositionIndex": 17, "CameraTimeout": 20000, "TimeFirst": false }, "640 AO-Name": "AnalogIO", "Vincent-D3-Command": "Close" }, "FrameKey-0-0-4": { "COM4-StopBits": "1", "528 AO-MaxVolts": "5.0000", "XLIGHT Excitation Wheel-State": "1", "470 Shutter-Name": "AnalogIO", "Core-Shutter": "520", "555 AO-Demo": "No", "XLIGHT Dichroic-State": "2", "XYStage-StepSize-Y": "0.1000", "ZPositionUm": 193.215, "XYStage-StepSize-X": "0.1000", "PRIME-FirmwareVersion": "20.28", "PRIME-ClearCycles": "2", "520-Name": "DA Shutter", "405 Shutter-Name": "AnalogIO", "PIEZO-StepSize": "0.0050", "PRIME-ClearMode": "Pre-Exposure", "470 AO-MinVolts": "0.0000", "405 AO-SupportsTriggering": "1", "CameraChannelIndex": 0, "XYStage-StartSpeed": "500.0000", "COM9-StopBits": "2", "405 AO-Description": "NI DAC", "COM4-DelayBetweenCharsMs": "0.0000", "445 AO-Block voltage": "false", "520 AO-TriggerInputLine": "/Dev1/PFI0", "555 AO-Description": "NI DAC", "520 AO-TriggerSampleRate": "100000", "COM4-Verbose": "1", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM4-Name": "COM4", "405 Shutter-Volts": "0.0000", "Vincent-D3-Port": "COM1", "PRIME-PP 4 MIN ADU AFFECTED": "200", "470 Shutter-MinVolts": "0.0000", "PRIME-PP 3 ENABLED": "Yes", "470 AO-Description": "NI DAC", "PRIME-Description": "PVCAM API device adapter", "520 Shutter-MaxVolts": "5.0000", "520 Shutter-Volts": "5.0000", "528 AO-Sequenceable": "1", "PRIME-Binning": "1x1", "555 Shutter-MaxVolts": "5.0000", "528 Shutter-TriggerSequenceLength": "1000", "405 Shutter-Block voltage": "false", "XLIGHT Spin motor-State": "1", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "Vincent-D3-Time to open (ms)": "35.0000", "Vincent-D3-Time to close (ms)": "35.0000", "445 AO-TriggerSampleRate": "100000", "640 Shutter-IOChannel": "Dev1/ao14", "555 AO-Sequenceable": "1", "520 AO-Name": "AnalogIO", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "470 AO-Block voltage": "false", "COM1-Verbose": "1", "COM9-AnswerTimeout": "10000.0000", "528 Shutter-Name": "AnalogIO", "PRIME-Trigger Timeout (secs)": "10", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "NextFrame": 0, "470 AO-TriggerSequenceLength": "1000", "PRIME-TransposeXY": "0", "445 AO-TriggerSequenceLength": "1000", "COM1-Name": "COM1", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "COM9-DataBits": "8", "COM1-AnswerTimeout": "500.0000", "Camera": "", "TE2000-Version": "V2.50-V2.50-V2.10", "528 Shutter-Volts": "0.0000", "XLIGHT Hub-Name": "XLIGHT Hub", "PIEZO-Description": "Ludl stage driver adapter", "PositionName": "Pos17", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSampleRate": "100000", "PRIME-FanSpeedSetpoint": "High", "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "405 AO-TriggerSampleRate": "100000", "470 Shutter-Block voltage": "false", "470-State": "0", "COM3-Parity": "None", "405 AO-Volts": "0.0000", "PRIME-TransposeMirrorY": "0", "PRIME-ChipName": "GS144BSI", "470 AO-Volts": "0.0000", "PRIME-TransposeMirrorX": "0", "445 Shutter-SupportsTriggering": "0", "470 Shutter-SupportsTriggering": "0", "XLIGHT Hub-Description": "XLIGHT hub", "PRIME-PreampOffLimit": "10000", "470 AO-Sequenceable": "1", "528 Shutter-MaxVolts": "5.0000", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "Core-XYStage": "XYStage", "470 AO-TriggerSampleRate": "100000", "PRIME-Timing-PreTriggerDelayNs": "24672000.0000", "528-DA Device": "528 Shutter", "Focus-Description": "Nikon TE2000 focus adapter", "FilterBlock-State": "5", "520 AO-Demo": "No", "COM3-DelayBetweenCharsMs": "0.0000", "470 Shutter-Volts": "0.0000", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "PRIME-PP 2 THRESHOLD": "125", "PRIME-PP 4 THRESHOLD": "75", "XYStage-Acceleration": "4.0000", "470 Shutter-Demo": "No", "PRIME-Name": "PMPCIECam00", "640-DA Device": "640 Shutter", "405 AO-Block voltage": "false", "ElapsedTime-ms": 718511, "PRIME-Offset": "100", "445 AO-IOChannel": "Dev1/ao1", "640 AO-MaxVolts": "5.0000", "PositionIndex": 17, "PRIME-PP 1 ENABLED": "Yes", "COM1-BaudRate": "9600", "640-State": "0", "COM3-Verbose": "1", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "LudlController-TransmissionDelay": "0", "PRIME-FullWellCapacity": "62000", "PRIME-SMARTStreamingEnabled": "No", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "640 Shutter-Description": "NI DAC", "528-Description": "DA device that is used as a shutter", "COM4-Description": "Serial port driver (boost:asio)", "405 Shutter-Description": "NI DAC", "TE2000-Name": "TE2000", "528 Shutter-Description": "NI DAC", "COM9-Description": "Serial port driver (boost:asio)", "LudlController-Name": "LudlController", "445 Shutter-TriggerSequenceLength": "1000", "PRIME-SerialNumber": "A18B203008", "445 AO-Volts": "0.0000", "PRIME-X-dimension": "1200", "PRIME-ShutterCloseDelay": "0", "XLIGHT Disk slider-State": "2", "Position": "Default", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 AO-IOChannel": "Dev1/ao5", "520 AO-IOChannel": "Dev1/ao4", "528 AO-TriggerSequenceLength": "1000", "405 Shutter-MaxVolts": "5.0000", "470-DA Device": "470 Shutter", "PRIME-FTCapable": "No", "Core-ChannelGroup": "Channels", "555 AO-SupportsTriggering": "0", "PRIME-Y-dimension": "1200", "Frame": 0, "555 Shutter-Sequenceable": "1", "PRIME-Timing-ExposureTimeNs": "499998000.0000", "Core-ImageProcessor": "", "528-State": "0", "520 AO-Block voltage": "false", "XLIGHT Excitation Wheel-Label": "CFP/YFP", "640 Shutter-Demo": "No", "COM3-StopBits": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "520 Shutter-IOChannel": "Dev1/ao11", "Width": 1200, "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-SupportsTriggering": "0", "640 AO-TriggerSampleRate": "100000", "520-Description": "DA device that is used as a shutter", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "405 Shutter-Sequenceable": "1", "405 Shutter-SupportsTriggering": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-TriggerSampleRate": "100000", "640 Shutter-MaxVolts": "5.0000", "555 AO-MaxVolts": "5.0000", "520 AO-TriggerSequenceLength": "1000", "COM3-BaudRate": "9600", "COM9-BaudRate": "9600", "Vincent-D3-Channel #": "Ch. #1", "PRIME-PMode": "Normal", "OpticalPath-Label": "Left(100)", "BitDepth": 16, "555 AO-TriggerSequenceLength": "1000", "445 Shutter-Description": "NI DAC", "405 AO-Sequenceable": "1", "PRIME-AcquisitionMethod": "Callbacks", "555 AO-Name": "AnalogIO", "PRIME-ShutterMode": "Pre-Exposure", "COM3-Handshaking": "Off", "445 AO-SupportsTriggering": "0", "ROI": "0-0-1200-1200", "COM9-Verbose": "1", "PRIME-Color": "OFF", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "PRIME-ShutterOpenDelay": "0", "COM1-Parity": "None", "Slice": 4, "SlicePosition": -10, "COM9-DelayBetweenCharsMs": "0.0000", "555-Name": "DA Shutter", "PIEZO-LudlSingleAxisName": "B", "XLIGHT Emission Wheel-Label": "565/24(G)", "640 Shutter-Name": "AnalogIO", "640 AO-Sequenceable": "1", "520 Shutter-Demo": "No", "528 Shutter-Demo": "No", "470-Name": "DA Shutter", "520-DA Device": "520 Shutter", "XYStage-Description": "Ludl XY stage driver adapter", "445 Shutter-MinVolts": "0.0000", "528 AO-TriggerSampleRate": "100000", "405 AO-MinVolts": "0.0000", "405 Shutter-TriggerSequenceLength": "1000", "Height": 1200, "555 AO-Block voltage": "false", "640 Shutter-Volts": "0.0000", "PixelType": "GRAY16", "445 Shutter-Block voltage": "false", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "520 Shutter-TriggerSequenceLength": "1000", "COM9-Name": "COM9", "Core-SLM": "", "555 AO-Volts": "0.0000", "555 Shutter-Block voltage": "false", "XYStage-Speed": "1800.0000", "520 AO-MaxVolts": "5.0000", "528 Shutter-SupportsTriggering": "0", "COM1-Description": "Serial port driver (boost:asio)", "PRIME-MetadataEnabled": "No", "520 Shutter-MinVolts": "0.0000", "470 Shutter-Sequenceable": "1", "PRIME-PreampDelay": "0", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-MinVolts": "0.0000", "ChannelIndex": 0, "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Core-AutoShutter": "1", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "Exposure-ms": 320, "PRIME-PP 2 ENABLED": "Yes", "640 Shutter-TriggerSampleRate": "100000", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "470 Shutter-IOChannel": "Dev1/ao10", "405 AO-Demo": "No", "PixelSizeUm": 1.1, "470 AO-Demo": "No", "470 Shutter-MaxVolts": "5.0000", "Vincent-D3-Name": "Vincent-D3", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FileName": "img_000000000_Il-G_004.tif", "LudlController-Reset": "Operate", "PRIME-Exposure": "320.00", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "445-Name": "DA Shutter", "555 Shutter-Name": "AnalogIO", "520 Shutter-TriggerSampleRate": "100000", "445 Shutter-IOChannel": "Dev1/ao9", "PRIME-TransposeCorrection": "0", "XYStage-StepSize": "0.1000", "445-Description": "DA device that is used as a shutter", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 AO-IOChannel": "Dev1/ao7", "520 Shutter-Name": "AnalogIO", "COM3-DataBits": "8", "640 AO-Volts": "0.0000", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "555 Shutter-IOChannel": "Dev1/ao13", "WaitInterval": null, "520 Shutter-Sequenceable": "1", "YPositionUm": -13012.300000000001, "445-State": "0", "445-DA Device": "445 Shutter", "PRIME-CircularBufferAutoSize": "ON", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "555 AO-TriggerSampleRate": "100000", "445 Shutter-Volts": "0.0000", "XLIGHT Hub-Port": "COM3", "Core-AutoFocus": "", "COM4-Handshaking": "Off", "528 Shutter-MinVolts": "0.0000", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "445 Shutter-Name": "AnalogIO", "528 Shutter-Sequenceable": "1", "XYStage-TransposeMirrorX": "0", "XYStage-TransposeMirrorY": "0", "PRIME-ReadoutRate": "100MHz 16bit", "XYStage-Name": "Stage", "640 AO-SupportsTriggering": "0", "640-Name": "DA Shutter", "640 Shutter-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "640 AO-Block voltage": "false", "445 Shutter-Sequenceable": "1", "470 AO-MaxVolts": "5.0000", "COM4-BaudRate": "9600", "470-Description": "DA device that is used as a shutter", "520 Shutter-Description": "NI DAC", "PRIME-CCDTemperature": "-14.9200", "Time": "2019-12-17 03:59:35 -0500", "640 AO-TriggerSequenceLength": "1000", "470 AO-Name": "AnalogIO", "XLIGHT Disk slider-Label": "Disk Pos 40um", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "Binning": "1x1", "TE2000-ModelType": "1", "COM3-AnswerTimeout": "500.0000", "555 Shutter-SupportsTriggering": "0", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "TE2000-Port": "COM4", "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Description": "NI DAC", "COM4-AnswerTimeout": "500.0000", "COM1-DelayBetweenCharsMs": "0.0000", "FilterBlock-Label": "Confocal", "445 AO-Name": "AnalogIO", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-Timing-ReadoutTimeNs": "24672000.0000", "528 AO-Demo": "No", "520 AO-Sequenceable": "1", "555-Description": "DA device that is used as a shutter", "405-DA Device": "405 Shutter", "405 AO-TriggerSequenceLength": "1000", "640 AO-MinVolts": "0.0000", "640 Shutter-Block voltage": "false", "FrameIndex": 0, "PRIME-Port": "CMOS", "555 AO-MinVolts": "0.0000", "COM3-Description": "Serial port driver (boost:asio)", "528 AO-Description": "NI DAC", "555 AO-IOChannel": "Dev1/ao6", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "555 AO-TriggerInputLine": "/Dev1/PFI0", "PRIME-ActualInterval-ms": "84.6200", "LudlController-Port": "COM9", "445 Shutter-Demo": "No", "PRIME-Trigger-Expose Out-Mux": "1", "SliceIndex": 4, "PRIME-CCDTemperatureSetPoint": "-15.0000", "405 Shutter-MinVolts": "0.0000", "PRIME-ExposeOutMode": "First Row", "AxisPositions": null, "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Volts": "0.0000", "528-Name": "DA Shutter", "555-State": "0", "XLIGHT Dichroic-Label": "CFP/YFP", "445 AO-Sequenceable": "1", "405-Name": "DA Shutter", "405 AO-IOChannel": "Dev1/ao0", "Core-Camera": "PRIME", "Focus-Name": "Focus", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "405 Shutter-IOChannel": "Dev1/ao8", "640 AO-Demo": "No", "640 Shutter-SupportsTriggering": "0", "445 AO-Demo": "No", "555-DA Device": "555 Shutter", "470 Shutter-Description": "NI DAC", "528 AO-Volts": "0.0000", "COM1-DataBits": "8", "Vincent-D3-Address": "x", "520 AO-SupportsTriggering": "0", "405-Description": "DA device that is used as a shutter", "640 Shutter-MinVolts": "0.0000", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "405 AO-Name": "AnalogIO", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "640 AO-TriggerInputLine": "/Dev1/PFI0", "405 AO-TriggerInputLine": "/Dev1/PFI0", "640-Description": "DA device that is used as a shutter", "445 Shutter-TriggerSampleRate": "100000", "470 Shutter-TriggerSequenceLength": "1000", "COM1-StopBits": "1", "520 Shutter-SupportsTriggering": "0", "405-State": "0", "Core-Initialize": "1", "XLIGHT Emission Wheel-State": "3", "520 AO-Description": "NI DAC", "528 AO-TriggerInputLine": "/Dev1/PFI0", "445 Shutter-MaxVolts": "5.0000", "COM9-Handshaking": "Off", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "XLIGHT Spin motor-Label": "On (spinning)", "PRIME-PP 5 ENABLED": "No", "UUID": "bab261cb-f138-449e-b627-ea0c98a994bc", "PRIME-TriggerMode": "Internal Trigger", "COM1-Handshaking": "Off", "PRIME-Timing-PostTriggerDelayNs": "49344000.0000", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "Focus-Position": "16732.9000", "528 Shutter-Block voltage": "false", "Channel": "Il-G", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "555 Shutter-Demo": "No", "405 Shutter-Demo": "No", "528 AO-Name": "AnalogIO", "520-State": "1", "Core-Galvo": "", "405 Shutter-TriggerSampleRate": "100000", "Focus-FocusStepSize_(nm)": "100", "PRIME-PixelType": "16bit", "COM4-DataBits": "8", "470 AO-IOChannel": "Dev1/ao2", "520 AO-MinVolts": "0.0000", "COM9-Parity": "None", "PRIME-CircularBufferEnabled": "ON", "PRIME-PP 6 Reset": "No", "640 AO-Description": "NI DAC", "Source": "PRIME", "445 AO-MinVolts": "0.0000", "PIEZO-Name": "Stage", "445 AO-Description": "NI DAC", "PRIME-CircularBufferFrameCount": "46", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "520 AO-Volts": "4.5000", "528 Shutter-TriggerSampleRate": "100000", "XPositionUm": 23269.100000000002, "OpticalPath-State": "4", "PRIME-PP 3 THRESHOLD": "80", "PRIME-PVCAM Version": "3.7.1", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Gain": "1-HDR", "640 Shutter-TriggerSequenceLength": "1000", "528 AO-SupportsTriggering": "0", "445 AO-TriggerInputLine": "/Dev1/PFI0", "OpticalPath-Name": "OpticalPath", "PRIME-PP 4 ENABLED": "Yes", "PRIME-PVCAM Adapter Version": "1.3.29", "COM3-Name": "COM3", "405 AO-MaxVolts": "5.0000", "520 Shutter-Block voltage": "false", "PRIME-Timing-ClearingTimeNs": "49344000.0000", "Core-Focus": "PIEZO", "528 AO-Block voltage": "false", "TE2000-Description": "Nikon TE2000 hub", "528 AO-MinVolts": "0.0000", "COM4-Parity": "None", "Summary": { "Slices": 11, "ChContrastMin": [ 0, 0, 0, 0, 0, 0 ], "PixelAspect": 1, "SlicesFirst": true, "Interval_ms": 0, "Time": "2019-12-17 03:59:33 -0500", "Prefix": "seq_7", "InitialPositionList": [ { "Label": "Pos0", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [149.91], "Focus": [16657.7], "XYStage": [ 71629.7, 19152.9 ] }, "GridColumnIndex": 0 }, { "Label": "Pos1", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [190.82], "Focus": [16485.4], "XYStage": [ 65702.3, 19396.600000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos2", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [194.69], "Focus": [16446.8], "XYStage": [ 59880.5, 19007.8 ] }, "GridColumnIndex": 0 }, { "Label": "Pos3", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [145.04], "Focus": [16446.6], "XYStage": [ 53616.9, 18818.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos4", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [169.57500000000002], "Focus": [16446.5], "XYStage": [ 47662.8, 18817.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos5", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [131.805], "Focus": [16446.4], "XYStage": [ 41918.8, 18298.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos6", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [113.145], "Focus": [16446.5], "XYStage": [ 36129.9, 18895.8 ] }, "GridColumnIndex": 0 }, { "Label": "Pos7", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [141.005], "Focus": [16446.6], "XYStage": [ 30299.100000000002, 18895.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos8", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [140.97], "Focus": [16446.5], "XYStage": [ 23952.100000000002, 19156 ] }, "GridColumnIndex": 0 }, { "Label": "Pos9", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [184.57500000000002], "Focus": [16677.9], "XYStage": [ 72866.8, -14809.300000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos10", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [167.95000000000002], "Focus": [16557.9], "XYStage": [ 66515.6, -14371.300000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos11", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [168], "Focus": [16618.9], "XYStage": [ 59724.3, -13964.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos12", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [203.205], "Focus": [16779.6], "XYStage": [ 53862.3, -13494.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos13", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [203.20000000000002], "Focus": [16749], "XYStage": [ 48108.3, -13805.400000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos14", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [203.19], "Focus": [16675.9], "XYStage": [ 41645, -13804.900000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos15", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [203.21], "Focus": [16675.7], "XYStage": [ 35352.6, -13805.6 ] }, "GridColumnIndex": 0 }, { "Label": "Pos16", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [203.225], "Focus": [16675.8], "XYStage": [ 29298, -13447.400000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos17", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [203.215], "Focus": [16675.8], "XYStage": [ 23269.100000000002, -13012.300000000001 ] }, "GridColumnIndex": 0 } ], "z-step_um": 10, "GridRow": 0, "BitDepth": 16, "Depth": 2, "Channels": 6, "Source": "Micro-Manager", "ChColors": [ -16711681, -256, -65281, -1, -1, -16776961 ], "MetadataVersion": 10, "KeepShutterOpenChannels": true, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536, 65536 ], "CustomIntervals_ms": [], "Height": 1200, "MicroManagerVersion": "1.4.23 20180219", "GridColumn": 0, "UUID": "3ad8801b-5699-40d6-813b-4ad840863e38", "PixelType": "GRAY16", "Width": 1200, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\Yan\\20191216-YC113-S11S12-miseq", "Frames": 1, "Comment": "", "UserName": "Nikon", "PixelSize_um": 1.1, "ROI": [ 0, 0, 1200, 1200 ], "Date": "2019-12-17", "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC", "P-DAPI-high" ], "KeepShutterOpenSlices": true, "IJType": 1, "ComputerName": "ZadorConfocal2", "Positions": 18, "PositionIndex": 17, "CameraTimeout": 20000, "TimeFirst": false }, "640 AO-Name": "AnalogIO", "Vincent-D3-Command": "Close" }, "FrameKey-0-0-5": { "COM4-StopBits": "1", "528 AO-MaxVolts": "5.0000", "XLIGHT Excitation Wheel-State": "1", "470 Shutter-Name": "AnalogIO", "Core-Shutter": "520", "555 AO-Demo": "No", "XLIGHT Dichroic-State": "2", "XYStage-StepSize-Y": "0.1000", "ZPositionUm": 203.215, "XYStage-StepSize-X": "0.1000", "PRIME-FirmwareVersion": "20.28", "PRIME-ClearCycles": "2", "520-Name": "DA Shutter", "405 Shutter-Name": "AnalogIO", "PIEZO-StepSize": "0.0050", "PRIME-ClearMode": "Pre-Exposure", "470 AO-MinVolts": "0.0000", "405 AO-SupportsTriggering": "1", "CameraChannelIndex": 0, "XYStage-StartSpeed": "500.0000", "COM9-StopBits": "2", "405 AO-Description": "NI DAC", "COM4-DelayBetweenCharsMs": "0.0000", "445 AO-Block voltage": "false", "520 AO-TriggerInputLine": "/Dev1/PFI0", "555 AO-Description": "NI DAC", "520 AO-TriggerSampleRate": "100000", "COM4-Verbose": "1", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM4-Name": "COM4", "405 Shutter-Volts": "0.0000", "Vincent-D3-Port": "COM1", "PRIME-PP 4 MIN ADU AFFECTED": "200", "470 Shutter-MinVolts": "0.0000", "PRIME-PP 3 ENABLED": "Yes", "470 AO-Description": "NI DAC", "PRIME-Description": "PVCAM API device adapter", "520 Shutter-MaxVolts": "5.0000", "520 Shutter-Volts": "5.0000", "528 AO-Sequenceable": "1", "PRIME-Binning": "1x1", "555 Shutter-MaxVolts": "5.0000", "528 Shutter-TriggerSequenceLength": "1000", "405 Shutter-Block voltage": "false", "XLIGHT Spin motor-State": "1", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "Vincent-D3-Time to open (ms)": "35.0000", "Vincent-D3-Time to close (ms)": "35.0000", "445 AO-TriggerSampleRate": "100000", "640 Shutter-IOChannel": "Dev1/ao14", "555 AO-Sequenceable": "1", "520 AO-Name": "AnalogIO", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "470 AO-Block voltage": "false", "COM1-Verbose": "1", "COM9-AnswerTimeout": "10000.0000", "528 Shutter-Name": "AnalogIO", "PRIME-Trigger Timeout (secs)": "10", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "NextFrame": 0, "470 AO-TriggerSequenceLength": "1000", "PRIME-TransposeXY": "0", "445 AO-TriggerSequenceLength": "1000", "COM1-Name": "COM1", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "COM9-DataBits": "8", "COM1-AnswerTimeout": "500.0000", "Camera": "", "TE2000-Version": "V2.50-V2.50-V2.10", "528 Shutter-Volts": "0.0000", "XLIGHT Hub-Name": "XLIGHT Hub", "PIEZO-Description": "Ludl stage driver adapter", "PositionName": "Pos17", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSampleRate": "100000", "PRIME-FanSpeedSetpoint": "High", "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "405 AO-TriggerSampleRate": "100000", "470 Shutter-Block voltage": "false", "470-State": "0", "COM3-Parity": "None", "405 AO-Volts": "0.0000", "PRIME-TransposeMirrorY": "0", "PRIME-ChipName": "GS144BSI", "470 AO-Volts": "0.0000", "PRIME-TransposeMirrorX": "0", "445 Shutter-SupportsTriggering": "0", "470 Shutter-SupportsTriggering": "0", "XLIGHT Hub-Description": "XLIGHT hub", "PRIME-PreampOffLimit": "10000", "470 AO-Sequenceable": "1", "528 Shutter-MaxVolts": "5.0000", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "Core-XYStage": "XYStage", "470 AO-TriggerSampleRate": "100000", "PRIME-Timing-PreTriggerDelayNs": "24672000.0000", "528-DA Device": "528 Shutter", "Focus-Description": "Nikon TE2000 focus adapter", "FilterBlock-State": "5", "520 AO-Demo": "No", "COM3-DelayBetweenCharsMs": "0.0000", "470 Shutter-Volts": "0.0000", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "PRIME-PP 2 THRESHOLD": "125", "PRIME-PP 4 THRESHOLD": "75", "XYStage-Acceleration": "4.0000", "470 Shutter-Demo": "No", "PRIME-Name": "PMPCIECam00", "640-DA Device": "640 Shutter", "405 AO-Block voltage": "false", "ElapsedTime-ms": 719123, "PRIME-Offset": "100", "445 AO-IOChannel": "Dev1/ao1", "640 AO-MaxVolts": "5.0000", "PositionIndex": 17, "PRIME-PP 1 ENABLED": "Yes", "COM1-BaudRate": "9600", "640-State": "0", "COM3-Verbose": "1", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "LudlController-TransmissionDelay": "0", "PRIME-FullWellCapacity": "62000", "PRIME-SMARTStreamingEnabled": "No", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "640 Shutter-Description": "NI DAC", "528-Description": "DA device that is used as a shutter", "COM4-Description": "Serial port driver (boost:asio)", "405 Shutter-Description": "NI DAC", "TE2000-Name": "TE2000", "528 Shutter-Description": "NI DAC", "COM9-Description": "Serial port driver (boost:asio)", "LudlController-Name": "LudlController", "445 Shutter-TriggerSequenceLength": "1000", "PRIME-SerialNumber": "A18B203008", "445 AO-Volts": "0.0000", "PRIME-X-dimension": "1200", "PRIME-ShutterCloseDelay": "0", "XLIGHT Disk slider-State": "2", "Position": "Default", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 AO-IOChannel": "Dev1/ao5", "520 AO-IOChannel": "Dev1/ao4", "528 AO-TriggerSequenceLength": "1000", "405 Shutter-MaxVolts": "5.0000", "470-DA Device": "470 Shutter", "PRIME-FTCapable": "No", "Core-ChannelGroup": "Channels", "555 AO-SupportsTriggering": "0", "PRIME-Y-dimension": "1200", "Frame": 0, "555 Shutter-Sequenceable": "1", "PRIME-Timing-ExposureTimeNs": "499998000.0000", "Core-ImageProcessor": "", "528-State": "0", "520 AO-Block voltage": "false", "XLIGHT Excitation Wheel-Label": "CFP/YFP", "640 Shutter-Demo": "No", "COM3-StopBits": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "520 Shutter-IOChannel": "Dev1/ao11", "Width": 1200, "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-SupportsTriggering": "0", "640 AO-TriggerSampleRate": "100000", "520-Description": "DA device that is used as a shutter", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "405 Shutter-Sequenceable": "1", "405 Shutter-SupportsTriggering": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-TriggerSampleRate": "100000", "640 Shutter-MaxVolts": "5.0000", "555 AO-MaxVolts": "5.0000", "520 AO-TriggerSequenceLength": "1000", "COM3-BaudRate": "9600", "COM9-BaudRate": "9600", "Vincent-D3-Channel #": "Ch. #1", "PRIME-PMode": "Normal", "OpticalPath-Label": "Left(100)", "BitDepth": 16, "555 AO-TriggerSequenceLength": "1000", "445 Shutter-Description": "NI DAC", "405 AO-Sequenceable": "1", "PRIME-AcquisitionMethod": "Callbacks", "555 AO-Name": "AnalogIO", "PRIME-ShutterMode": "Pre-Exposure", "COM3-Handshaking": "Off", "445 AO-SupportsTriggering": "0", "ROI": "0-0-1200-1200", "COM9-Verbose": "1", "PRIME-Color": "OFF", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "PRIME-ShutterOpenDelay": "0", "COM1-Parity": "None", "Slice": 5, "SlicePosition": 0, "COM9-DelayBetweenCharsMs": "0.0000", "555-Name": "DA Shutter", "PIEZO-LudlSingleAxisName": "B", "XLIGHT Emission Wheel-Label": "565/24(G)", "640 Shutter-Name": "AnalogIO", "640 AO-Sequenceable": "1", "520 Shutter-Demo": "No", "528 Shutter-Demo": "No", "470-Name": "DA Shutter", "520-DA Device": "520 Shutter", "XYStage-Description": "Ludl XY stage driver adapter", "445 Shutter-MinVolts": "0.0000", "528 AO-TriggerSampleRate": "100000", "405 AO-MinVolts": "0.0000", "405 Shutter-TriggerSequenceLength": "1000", "Height": 1200, "555 AO-Block voltage": "false", "640 Shutter-Volts": "0.0000", "PixelType": "GRAY16", "445 Shutter-Block voltage": "false", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "520 Shutter-TriggerSequenceLength": "1000", "COM9-Name": "COM9", "Core-SLM": "", "555 AO-Volts": "0.0000", "555 Shutter-Block voltage": "false", "XYStage-Speed": "1800.0000", "520 AO-MaxVolts": "5.0000", "528 Shutter-SupportsTriggering": "0", "COM1-Description": "Serial port driver (boost:asio)", "PRIME-MetadataEnabled": "No", "520 Shutter-MinVolts": "0.0000", "470 Shutter-Sequenceable": "1", "PRIME-PreampDelay": "0", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-MinVolts": "0.0000", "ChannelIndex": 0, "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Core-AutoShutter": "1", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "Exposure-ms": 320, "PRIME-PP 2 ENABLED": "Yes", "640 Shutter-TriggerSampleRate": "100000", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "470 Shutter-IOChannel": "Dev1/ao10", "405 AO-Demo": "No", "PixelSizeUm": 1.1, "470 AO-Demo": "No", "470 Shutter-MaxVolts": "5.0000", "Vincent-D3-Name": "Vincent-D3", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FileName": "img_000000000_Il-G_005.tif", "LudlController-Reset": "Operate", "PRIME-Exposure": "320.00", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "445-Name": "DA Shutter", "555 Shutter-Name": "AnalogIO", "520 Shutter-TriggerSampleRate": "100000", "445 Shutter-IOChannel": "Dev1/ao9", "PRIME-TransposeCorrection": "0", "XYStage-StepSize": "0.1000", "445-Description": "DA device that is used as a shutter", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 AO-IOChannel": "Dev1/ao7", "520 Shutter-Name": "AnalogIO", "COM3-DataBits": "8", "640 AO-Volts": "0.0000", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "555 Shutter-IOChannel": "Dev1/ao13", "WaitInterval": null, "520 Shutter-Sequenceable": "1", "YPositionUm": -13012.300000000001, "445-State": "0", "445-DA Device": "445 Shutter", "PRIME-CircularBufferAutoSize": "ON", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "555 AO-TriggerSampleRate": "100000", "445 Shutter-Volts": "0.0000", "XLIGHT Hub-Port": "COM3", "Core-AutoFocus": "", "COM4-Handshaking": "Off", "528 Shutter-MinVolts": "0.0000", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "445 Shutter-Name": "AnalogIO", "528 Shutter-Sequenceable": "1", "XYStage-TransposeMirrorX": "0", "XYStage-TransposeMirrorY": "0", "PRIME-ReadoutRate": "100MHz 16bit", "XYStage-Name": "Stage", "640 AO-SupportsTriggering": "0", "640-Name": "DA Shutter", "640 Shutter-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "640 AO-Block voltage": "false", "445 Shutter-Sequenceable": "1", "470 AO-MaxVolts": "5.0000", "COM4-BaudRate": "9600", "470-Description": "DA device that is used as a shutter", "520 Shutter-Description": "NI DAC", "PRIME-CCDTemperature": "-14.9200", "Time": "2019-12-17 03:59:36 -0500", "640 AO-TriggerSequenceLength": "1000", "470 AO-Name": "AnalogIO", "XLIGHT Disk slider-Label": "Disk Pos 40um", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "Binning": "1x1", "TE2000-ModelType": "1", "COM3-AnswerTimeout": "500.0000", "555 Shutter-SupportsTriggering": "0", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "TE2000-Port": "COM4", "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Description": "NI DAC", "COM4-AnswerTimeout": "500.0000", "COM1-DelayBetweenCharsMs": "0.0000", "FilterBlock-Label": "Confocal", "445 AO-Name": "AnalogIO", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-Timing-ReadoutTimeNs": "24672000.0000", "528 AO-Demo": "No", "520 AO-Sequenceable": "1", "555-Description": "DA device that is used as a shutter", "405-DA Device": "405 Shutter", "405 AO-TriggerSequenceLength": "1000", "640 AO-MinVolts": "0.0000", "640 Shutter-Block voltage": "false", "FrameIndex": 0, "PRIME-Port": "CMOS", "555 AO-MinVolts": "0.0000", "COM3-Description": "Serial port driver (boost:asio)", "528 AO-Description": "NI DAC", "555 AO-IOChannel": "Dev1/ao6", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "555 AO-TriggerInputLine": "/Dev1/PFI0", "PRIME-ActualInterval-ms": "84.6200", "LudlController-Port": "COM9", "445 Shutter-Demo": "No", "PRIME-Trigger-Expose Out-Mux": "1", "SliceIndex": 5, "PRIME-CCDTemperatureSetPoint": "-15.0000", "405 Shutter-MinVolts": "0.0000", "PRIME-ExposeOutMode": "First Row", "AxisPositions": null, "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Volts": "0.0000", "528-Name": "DA Shutter", "555-State": "0", "XLIGHT Dichroic-Label": "CFP/YFP", "445 AO-Sequenceable": "1", "405-Name": "DA Shutter", "405 AO-IOChannel": "Dev1/ao0", "Core-Camera": "PRIME", "Focus-Name": "Focus", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "405 Shutter-IOChannel": "Dev1/ao8", "640 AO-Demo": "No", "640 Shutter-SupportsTriggering": "0", "445 AO-Demo": "No", "555-DA Device": "555 Shutter", "470 Shutter-Description": "NI DAC", "528 AO-Volts": "0.0000", "COM1-DataBits": "8", "Vincent-D3-Address": "x", "520 AO-SupportsTriggering": "0", "405-Description": "DA device that is used as a shutter", "640 Shutter-MinVolts": "0.0000", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "405 AO-Name": "AnalogIO", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "640 AO-TriggerInputLine": "/Dev1/PFI0", "405 AO-TriggerInputLine": "/Dev1/PFI0", "640-Description": "DA device that is used as a shutter", "445 Shutter-TriggerSampleRate": "100000", "470 Shutter-TriggerSequenceLength": "1000", "COM1-StopBits": "1", "520 Shutter-SupportsTriggering": "0", "405-State": "0", "Core-Initialize": "1", "XLIGHT Emission Wheel-State": "3", "520 AO-Description": "NI DAC", "528 AO-TriggerInputLine": "/Dev1/PFI0", "445 Shutter-MaxVolts": "5.0000", "COM9-Handshaking": "Off", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "XLIGHT Spin motor-Label": "On (spinning)", "PRIME-PP 5 ENABLED": "No", "UUID": "1be420ab-e2a5-4129-9bc9-235df6b70b17", "PRIME-TriggerMode": "Internal Trigger", "COM1-Handshaking": "Off", "PRIME-Timing-PostTriggerDelayNs": "49344000.0000", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "Focus-Position": "16732.9000", "528 Shutter-Block voltage": "false", "Channel": "Il-G", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "555 Shutter-Demo": "No", "405 Shutter-Demo": "No", "528 AO-Name": "AnalogIO", "520-State": "1", "Core-Galvo": "", "405 Shutter-TriggerSampleRate": "100000", "Focus-FocusStepSize_(nm)": "100", "PRIME-PixelType": "16bit", "COM4-DataBits": "8", "470 AO-IOChannel": "Dev1/ao2", "520 AO-MinVolts": "0.0000", "COM9-Parity": "None", "PRIME-CircularBufferEnabled": "ON", "PRIME-PP 6 Reset": "No", "640 AO-Description": "NI DAC", "Source": "PRIME", "445 AO-MinVolts": "0.0000", "PIEZO-Name": "Stage", "445 AO-Description": "NI DAC", "PRIME-CircularBufferFrameCount": "46", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "520 AO-Volts": "4.5000", "528 Shutter-TriggerSampleRate": "100000", "XPositionUm": 23269.100000000002, "OpticalPath-State": "4", "PRIME-PP 3 THRESHOLD": "80", "PRIME-PVCAM Version": "3.7.1", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Gain": "1-HDR", "640 Shutter-TriggerSequenceLength": "1000", "528 AO-SupportsTriggering": "0", "445 AO-TriggerInputLine": "/Dev1/PFI0", "OpticalPath-Name": "OpticalPath", "PRIME-PP 4 ENABLED": "Yes", "PRIME-PVCAM Adapter Version": "1.3.29", "COM3-Name": "COM3", "405 AO-MaxVolts": "5.0000", "520 Shutter-Block voltage": "false", "PRIME-Timing-ClearingTimeNs": "49344000.0000", "Core-Focus": "PIEZO", "528 AO-Block voltage": "false", "TE2000-Description": "Nikon TE2000 hub", "528 AO-MinVolts": "0.0000", "COM4-Parity": "None", "Summary": { "Slices": 11, "ChContrastMin": [ 0, 0, 0, 0, 0, 0 ], "PixelAspect": 1, "SlicesFirst": true, "Interval_ms": 0, "Time": "2019-12-17 03:59:33 -0500", "Prefix": "seq_7", "InitialPositionList": [ { "Label": "Pos0", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [149.91], "Focus": [16657.7], "XYStage": [ 71629.7, 19152.9 ] }, "GridColumnIndex": 0 }, { "Label": "Pos1", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [190.82], "Focus": [16485.4], "XYStage": [ 65702.3, 19396.600000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos2", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [194.69], "Focus": [16446.8], "XYStage": [ 59880.5, 19007.8 ] }, "GridColumnIndex": 0 }, { "Label": "Pos3", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [145.04], "Focus": [16446.6], "XYStage": [ 53616.9, 18818.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos4", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [169.57500000000002], "Focus": [16446.5], "XYStage": [ 47662.8, 18817.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos5", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [131.805], "Focus": [16446.4], "XYStage": [ 41918.8, 18298.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos6", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [113.145], "Focus": [16446.5], "XYStage": [ 36129.9, 18895.8 ] }, "GridColumnIndex": 0 }, { "Label": "Pos7", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [141.005], "Focus": [16446.6], "XYStage": [ 30299.100000000002, 18895.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos8", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [140.97], "Focus": [16446.5], "XYStage": [ 23952.100000000002, 19156 ] }, "GridColumnIndex": 0 }, { "Label": "Pos9", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [184.57500000000002], "Focus": [16677.9], "XYStage": [ 72866.8, -14809.300000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos10", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [167.95000000000002], "Focus": [16557.9], "XYStage": [ 66515.6, -14371.300000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos11", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [168], "Focus": [16618.9], "XYStage": [ 59724.3, -13964.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos12", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [203.205], "Focus": [16779.6], "XYStage": [ 53862.3, -13494.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos13", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [203.20000000000002], "Focus": [16749], "XYStage": [ 48108.3, -13805.400000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos14", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [203.19], "Focus": [16675.9], "XYStage": [ 41645, -13804.900000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos15", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [203.21], "Focus": [16675.7], "XYStage": [ 35352.6, -13805.6 ] }, "GridColumnIndex": 0 }, { "Label": "Pos16", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [203.225], "Focus": [16675.8], "XYStage": [ 29298, -13447.400000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos17", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [203.215], "Focus": [16675.8], "XYStage": [ 23269.100000000002, -13012.300000000001 ] }, "GridColumnIndex": 0 } ], "z-step_um": 10, "GridRow": 0, "BitDepth": 16, "Depth": 2, "Channels": 6, "Source": "Micro-Manager", "ChColors": [ -16711681, -256, -65281, -1, -1, -16776961 ], "MetadataVersion": 10, "KeepShutterOpenChannels": true, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536, 65536 ], "CustomIntervals_ms": [], "Height": 1200, "MicroManagerVersion": "1.4.23 20180219", "GridColumn": 0, "UUID": "3ad8801b-5699-40d6-813b-4ad840863e38", "PixelType": "GRAY16", "Width": 1200, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\Yan\\20191216-YC113-S11S12-miseq", "Frames": 1, "Comment": "", "UserName": "Nikon", "PixelSize_um": 1.1, "ROI": [ 0, 0, 1200, 1200 ], "Date": "2019-12-17", "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC", "P-DAPI-high" ], "KeepShutterOpenSlices": true, "IJType": 1, "ComputerName": "ZadorConfocal2", "Positions": 18, "PositionIndex": 17, "CameraTimeout": 20000, "TimeFirst": false }, "640 AO-Name": "AnalogIO", "Vincent-D3-Command": "Close" }, "FrameKey-0-0-6": { "COM4-StopBits": "1", "528 AO-MaxVolts": "5.0000", "XLIGHT Excitation Wheel-State": "1", "470 Shutter-Name": "AnalogIO", "Core-Shutter": "520", "555 AO-Demo": "No", "XLIGHT Dichroic-State": "2", "XYStage-StepSize-Y": "0.1000", "ZPositionUm": 213.215, "XYStage-StepSize-X": "0.1000", "PRIME-FirmwareVersion": "20.28", "PRIME-ClearCycles": "2", "520-Name": "DA Shutter", "405 Shutter-Name": "AnalogIO", "PIEZO-StepSize": "0.0050", "PRIME-ClearMode": "Pre-Exposure", "470 AO-MinVolts": "0.0000", "405 AO-SupportsTriggering": "1", "CameraChannelIndex": 0, "XYStage-StartSpeed": "500.0000", "COM9-StopBits": "2", "405 AO-Description": "NI DAC", "COM4-DelayBetweenCharsMs": "0.0000", "445 AO-Block voltage": "false", "520 AO-TriggerInputLine": "/Dev1/PFI0", "555 AO-Description": "NI DAC", "520 AO-TriggerSampleRate": "100000", "COM4-Verbose": "1", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM4-Name": "COM4", "405 Shutter-Volts": "0.0000", "Vincent-D3-Port": "COM1", "PRIME-PP 4 MIN ADU AFFECTED": "200", "470 Shutter-MinVolts": "0.0000", "PRIME-PP 3 ENABLED": "Yes", "470 AO-Description": "NI DAC", "PRIME-Description": "PVCAM API device adapter", "520 Shutter-MaxVolts": "5.0000", "520 Shutter-Volts": "5.0000", "528 AO-Sequenceable": "1", "PRIME-Binning": "1x1", "555 Shutter-MaxVolts": "5.0000", "528 Shutter-TriggerSequenceLength": "1000", "405 Shutter-Block voltage": "false", "XLIGHT Spin motor-State": "1", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "Vincent-D3-Time to open (ms)": "35.0000", "Vincent-D3-Time to close (ms)": "35.0000", "445 AO-TriggerSampleRate": "100000", "640 Shutter-IOChannel": "Dev1/ao14", "555 AO-Sequenceable": "1", "520 AO-Name": "AnalogIO", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "470 AO-Block voltage": "false", "COM1-Verbose": "1", "COM9-AnswerTimeout": "10000.0000", "528 Shutter-Name": "AnalogIO", "PRIME-Trigger Timeout (secs)": "10", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "NextFrame": 0, "470 AO-TriggerSequenceLength": "1000", "PRIME-TransposeXY": "0", "445 AO-TriggerSequenceLength": "1000", "COM1-Name": "COM1", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "COM9-DataBits": "8", "COM1-AnswerTimeout": "500.0000", "Camera": "", "TE2000-Version": "V2.50-V2.50-V2.10", "528 Shutter-Volts": "0.0000", "XLIGHT Hub-Name": "XLIGHT Hub", "PIEZO-Description": "Ludl stage driver adapter", "PositionName": "Pos17", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSampleRate": "100000", "PRIME-FanSpeedSetpoint": "High", "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "405 AO-TriggerSampleRate": "100000", "470 Shutter-Block voltage": "false", "470-State": "0", "COM3-Parity": "None", "405 AO-Volts": "0.0000", "PRIME-TransposeMirrorY": "0", "PRIME-ChipName": "GS144BSI", "470 AO-Volts": "0.0000", "PRIME-TransposeMirrorX": "0", "445 Shutter-SupportsTriggering": "0", "470 Shutter-SupportsTriggering": "0", "XLIGHT Hub-Description": "XLIGHT hub", "PRIME-PreampOffLimit": "10000", "470 AO-Sequenceable": "1", "528 Shutter-MaxVolts": "5.0000", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "Core-XYStage": "XYStage", "470 AO-TriggerSampleRate": "100000", "PRIME-Timing-PreTriggerDelayNs": "24672000.0000", "528-DA Device": "528 Shutter", "Focus-Description": "Nikon TE2000 focus adapter", "FilterBlock-State": "5", "520 AO-Demo": "No", "COM3-DelayBetweenCharsMs": "0.0000", "470 Shutter-Volts": "0.0000", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "PRIME-PP 2 THRESHOLD": "125", "PRIME-PP 4 THRESHOLD": "75", "XYStage-Acceleration": "4.0000", "470 Shutter-Demo": "No", "PRIME-Name": "PMPCIECam00", "640-DA Device": "640 Shutter", "405 AO-Block voltage": "false", "ElapsedTime-ms": 719694, "PRIME-Offset": "100", "445 AO-IOChannel": "Dev1/ao1", "640 AO-MaxVolts": "5.0000", "PositionIndex": 17, "PRIME-PP 1 ENABLED": "Yes", "COM1-BaudRate": "9600", "640-State": "0", "COM3-Verbose": "1", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "LudlController-TransmissionDelay": "0", "PRIME-FullWellCapacity": "62000", "PRIME-SMARTStreamingEnabled": "No", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "640 Shutter-Description": "NI DAC", "528-Description": "DA device that is used as a shutter", "COM4-Description": "Serial port driver (boost:asio)", "405 Shutter-Description": "NI DAC", "TE2000-Name": "TE2000", "528 Shutter-Description": "NI DAC", "COM9-Description": "Serial port driver (boost:asio)", "LudlController-Name": "LudlController", "445 Shutter-TriggerSequenceLength": "1000", "PRIME-SerialNumber": "A18B203008", "445 AO-Volts": "0.0000", "PRIME-X-dimension": "1200", "PRIME-ShutterCloseDelay": "0", "XLIGHT Disk slider-State": "2", "Position": "Default", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 AO-IOChannel": "Dev1/ao5", "520 AO-IOChannel": "Dev1/ao4", "528 AO-TriggerSequenceLength": "1000", "405 Shutter-MaxVolts": "5.0000", "470-DA Device": "470 Shutter", "PRIME-FTCapable": "No", "Core-ChannelGroup": "Channels", "555 AO-SupportsTriggering": "0", "PRIME-Y-dimension": "1200", "Frame": 0, "555 Shutter-Sequenceable": "1", "PRIME-Timing-ExposureTimeNs": "499998000.0000", "Core-ImageProcessor": "", "528-State": "0", "520 AO-Block voltage": "false", "XLIGHT Excitation Wheel-Label": "CFP/YFP", "640 Shutter-Demo": "No", "COM3-StopBits": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "520 Shutter-IOChannel": "Dev1/ao11", "Width": 1200, "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-SupportsTriggering": "0", "640 AO-TriggerSampleRate": "100000", "520-Description": "DA device that is used as a shutter", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "405 Shutter-Sequenceable": "1", "405 Shutter-SupportsTriggering": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-TriggerSampleRate": "100000", "640 Shutter-MaxVolts": "5.0000", "555 AO-MaxVolts": "5.0000", "520 AO-TriggerSequenceLength": "1000", "COM3-BaudRate": "9600", "COM9-BaudRate": "9600", "Vincent-D3-Channel #": "Ch. #1", "PRIME-PMode": "Normal", "OpticalPath-Label": "Left(100)", "BitDepth": 16, "555 AO-TriggerSequenceLength": "1000", "445 Shutter-Description": "NI DAC", "405 AO-Sequenceable": "1", "PRIME-AcquisitionMethod": "Callbacks", "555 AO-Name": "AnalogIO", "PRIME-ShutterMode": "Pre-Exposure", "COM3-Handshaking": "Off", "445 AO-SupportsTriggering": "0", "ROI": "0-0-1200-1200", "COM9-Verbose": "1", "PRIME-Color": "OFF", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "PRIME-ShutterOpenDelay": "0", "COM1-Parity": "None", "Slice": 6, "SlicePosition": 10, "COM9-DelayBetweenCharsMs": "0.0000", "555-Name": "DA Shutter", "PIEZO-LudlSingleAxisName": "B", "XLIGHT Emission Wheel-Label": "565/24(G)", "640 Shutter-Name": "AnalogIO", "640 AO-Sequenceable": "1", "520 Shutter-Demo": "No", "528 Shutter-Demo": "No", "470-Name": "DA Shutter", "520-DA Device": "520 Shutter", "XYStage-Description": "Ludl XY stage driver adapter", "445 Shutter-MinVolts": "0.0000", "528 AO-TriggerSampleRate": "100000", "405 AO-MinVolts": "0.0000", "405 Shutter-TriggerSequenceLength": "1000", "Height": 1200, "555 AO-Block voltage": "false", "640 Shutter-Volts": "0.0000", "PixelType": "GRAY16", "445 Shutter-Block voltage": "false", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "520 Shutter-TriggerSequenceLength": "1000", "COM9-Name": "COM9", "Core-SLM": "", "555 AO-Volts": "0.0000", "555 Shutter-Block voltage": "false", "XYStage-Speed": "1800.0000", "520 AO-MaxVolts": "5.0000", "528 Shutter-SupportsTriggering": "0", "COM1-Description": "Serial port driver (boost:asio)", "PRIME-MetadataEnabled": "No", "520 Shutter-MinVolts": "0.0000", "470 Shutter-Sequenceable": "1", "PRIME-PreampDelay": "0", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-MinVolts": "0.0000", "ChannelIndex": 0, "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Core-AutoShutter": "1", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "Exposure-ms": 320, "PRIME-PP 2 ENABLED": "Yes", "640 Shutter-TriggerSampleRate": "100000", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "470 Shutter-IOChannel": "Dev1/ao10", "405 AO-Demo": "No", "PixelSizeUm": 1.1, "470 AO-Demo": "No", "470 Shutter-MaxVolts": "5.0000", "Vincent-D3-Name": "Vincent-D3", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FileName": "img_000000000_Il-G_006.tif", "LudlController-Reset": "Operate", "PRIME-Exposure": "320.00", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "445-Name": "DA Shutter", "555 Shutter-Name": "AnalogIO", "520 Shutter-TriggerSampleRate": "100000", "445 Shutter-IOChannel": "Dev1/ao9", "PRIME-TransposeCorrection": "0", "XYStage-StepSize": "0.1000", "445-Description": "DA device that is used as a shutter", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 AO-IOChannel": "Dev1/ao7", "520 Shutter-Name": "AnalogIO", "COM3-DataBits": "8", "640 AO-Volts": "0.0000", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "555 Shutter-IOChannel": "Dev1/ao13", "WaitInterval": null, "520 Shutter-Sequenceable": "1", "YPositionUm": -13012.300000000001, "445-State": "0", "445-DA Device": "445 Shutter", "PRIME-CircularBufferAutoSize": "ON", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "555 AO-TriggerSampleRate": "100000", "445 Shutter-Volts": "0.0000", "XLIGHT Hub-Port": "COM3", "Core-AutoFocus": "", "COM4-Handshaking": "Off", "528 Shutter-MinVolts": "0.0000", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "445 Shutter-Name": "AnalogIO", "528 Shutter-Sequenceable": "1", "XYStage-TransposeMirrorX": "0", "XYStage-TransposeMirrorY": "0", "PRIME-ReadoutRate": "100MHz 16bit", "XYStage-Name": "Stage", "640 AO-SupportsTriggering": "0", "640-Name": "DA Shutter", "640 Shutter-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "640 AO-Block voltage": "false", "445 Shutter-Sequenceable": "1", "470 AO-MaxVolts": "5.0000", "COM4-BaudRate": "9600", "470-Description": "DA device that is used as a shutter", "520 Shutter-Description": "NI DAC", "PRIME-CCDTemperature": "-14.9200", "Time": "2019-12-17 03:59:36 -0500", "640 AO-TriggerSequenceLength": "1000", "470 AO-Name": "AnalogIO", "XLIGHT Disk slider-Label": "Disk Pos 40um", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "Binning": "1x1", "TE2000-ModelType": "1", "COM3-AnswerTimeout": "500.0000", "555 Shutter-SupportsTriggering": "0", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "TE2000-Port": "COM4", "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Description": "NI DAC", "COM4-AnswerTimeout": "500.0000", "COM1-DelayBetweenCharsMs": "0.0000", "FilterBlock-Label": "Confocal", "445 AO-Name": "AnalogIO", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-Timing-ReadoutTimeNs": "24672000.0000", "528 AO-Demo": "No", "520 AO-Sequenceable": "1", "555-Description": "DA device that is used as a shutter", "405-DA Device": "405 Shutter", "405 AO-TriggerSequenceLength": "1000", "640 AO-MinVolts": "0.0000", "640 Shutter-Block voltage": "false", "FrameIndex": 0, "PRIME-Port": "CMOS", "555 AO-MinVolts": "0.0000", "COM3-Description": "Serial port driver (boost:asio)", "528 AO-Description": "NI DAC", "555 AO-IOChannel": "Dev1/ao6", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "555 AO-TriggerInputLine": "/Dev1/PFI0", "PRIME-ActualInterval-ms": "84.6200", "LudlController-Port": "COM9", "445 Shutter-Demo": "No", "PRIME-Trigger-Expose Out-Mux": "1", "SliceIndex": 6, "PRIME-CCDTemperatureSetPoint": "-15.0000", "405 Shutter-MinVolts": "0.0000", "PRIME-ExposeOutMode": "First Row", "AxisPositions": null, "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Volts": "0.0000", "528-Name": "DA Shutter", "555-State": "0", "XLIGHT Dichroic-Label": "CFP/YFP", "445 AO-Sequenceable": "1", "405-Name": "DA Shutter", "405 AO-IOChannel": "Dev1/ao0", "Core-Camera": "PRIME", "Focus-Name": "Focus", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "405 Shutter-IOChannel": "Dev1/ao8", "640 AO-Demo": "No", "640 Shutter-SupportsTriggering": "0", "445 AO-Demo": "No", "555-DA Device": "555 Shutter", "470 Shutter-Description": "NI DAC", "528 AO-Volts": "0.0000", "COM1-DataBits": "8", "Vincent-D3-Address": "x", "520 AO-SupportsTriggering": "0", "405-Description": "DA device that is used as a shutter", "640 Shutter-MinVolts": "0.0000", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "405 AO-Name": "AnalogIO", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "640 AO-TriggerInputLine": "/Dev1/PFI0", "405 AO-TriggerInputLine": "/Dev1/PFI0", "640-Description": "DA device that is used as a shutter", "445 Shutter-TriggerSampleRate": "100000", "470 Shutter-TriggerSequenceLength": "1000", "COM1-StopBits": "1", "520 Shutter-SupportsTriggering": "0", "405-State": "0", "Core-Initialize": "1", "XLIGHT Emission Wheel-State": "3", "520 AO-Description": "NI DAC", "528 AO-TriggerInputLine": "/Dev1/PFI0", "445 Shutter-MaxVolts": "5.0000", "COM9-Handshaking": "Off", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "XLIGHT Spin motor-Label": "On (spinning)", "PRIME-PP 5 ENABLED": "No", "UUID": "d3db4f3c-68f5-4468-bfc2-ac2b67656ce0", "PRIME-TriggerMode": "Internal Trigger", "COM1-Handshaking": "Off", "PRIME-Timing-PostTriggerDelayNs": "49344000.0000", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "Focus-Position": "16732.9000", "528 Shutter-Block voltage": "false", "Channel": "Il-G", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "555 Shutter-Demo": "No", "405 Shutter-Demo": "No", "528 AO-Name": "AnalogIO", "520-State": "1", "Core-Galvo": "", "405 Shutter-TriggerSampleRate": "100000", "Focus-FocusStepSize_(nm)": "100", "PRIME-PixelType": "16bit", "COM4-DataBits": "8", "470 AO-IOChannel": "Dev1/ao2", "520 AO-MinVolts": "0.0000", "COM9-Parity": "None", "PRIME-CircularBufferEnabled": "ON", "PRIME-PP 6 Reset": "No", "640 AO-Description": "NI DAC", "Source": "PRIME", "445 AO-MinVolts": "0.0000", "PIEZO-Name": "Stage", "445 AO-Description": "NI DAC", "PRIME-CircularBufferFrameCount": "46", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "520 AO-Volts": "4.5000", "528 Shutter-TriggerSampleRate": "100000", "XPositionUm": 23269.100000000002, "OpticalPath-State": "4", "PRIME-PP 3 THRESHOLD": "80", "PRIME-PVCAM Version": "3.7.1", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Gain": "1-HDR", "640 Shutter-TriggerSequenceLength": "1000", "528 AO-SupportsTriggering": "0", "445 AO-TriggerInputLine": "/Dev1/PFI0", "OpticalPath-Name": "OpticalPath", "PRIME-PP 4 ENABLED": "Yes", "PRIME-PVCAM Adapter Version": "1.3.29", "COM3-Name": "COM3", "405 AO-MaxVolts": "5.0000", "520 Shutter-Block voltage": "false", "PRIME-Timing-ClearingTimeNs": "49344000.0000", "Core-Focus": "PIEZO", "528 AO-Block voltage": "false", "TE2000-Description": "Nikon TE2000 hub", "528 AO-MinVolts": "0.0000", "COM4-Parity": "None", "Summary": { "Slices": 11, "ChContrastMin": [ 0, 0, 0, 0, 0, 0 ], "PixelAspect": 1, "SlicesFirst": true, "Interval_ms": 0, "Time": "2019-12-17 03:59:33 -0500", "Prefix": "seq_7", "InitialPositionList": [ { "Label": "Pos0", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [149.91], "Focus": [16657.7], "XYStage": [ 71629.7, 19152.9 ] }, "GridColumnIndex": 0 }, { "Label": "Pos1", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [190.82], "Focus": [16485.4], "XYStage": [ 65702.3, 19396.600000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos2", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [194.69], "Focus": [16446.8], "XYStage": [ 59880.5, 19007.8 ] }, "GridColumnIndex": 0 }, { "Label": "Pos3", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [145.04], "Focus": [16446.6], "XYStage": [ 53616.9, 18818.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos4", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [169.57500000000002], "Focus": [16446.5], "XYStage": [ 47662.8, 18817.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos5", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [131.805], "Focus": [16446.4], "XYStage": [ 41918.8, 18298.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos6", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [113.145], "Focus": [16446.5], "XYStage": [ 36129.9, 18895.8 ] }, "GridColumnIndex": 0 }, { "Label": "Pos7", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [141.005], "Focus": [16446.6], "XYStage": [ 30299.100000000002, 18895.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos8", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [140.97], "Focus": [16446.5], "XYStage": [ 23952.100000000002, 19156 ] }, "GridColumnIndex": 0 }, { "Label": "Pos9", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [184.57500000000002], "Focus": [16677.9], "XYStage": [ 72866.8, -14809.300000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos10", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [167.95000000000002], "Focus": [16557.9], "XYStage": [ 66515.6, -14371.300000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos11", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [168], "Focus": [16618.9], "XYStage": [ 59724.3, -13964.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos12", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [203.205], "Focus": [16779.6], "XYStage": [ 53862.3, -13494.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos13", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [203.20000000000002], "Focus": [16749], "XYStage": [ 48108.3, -13805.400000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos14", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [203.19], "Focus": [16675.9], "XYStage": [ 41645, -13804.900000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos15", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [203.21], "Focus": [16675.7], "XYStage": [ 35352.6, -13805.6 ] }, "GridColumnIndex": 0 }, { "Label": "Pos16", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [203.225], "Focus": [16675.8], "XYStage": [ 29298, -13447.400000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos17", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [203.215], "Focus": [16675.8], "XYStage": [ 23269.100000000002, -13012.300000000001 ] }, "GridColumnIndex": 0 } ], "z-step_um": 10, "GridRow": 0, "BitDepth": 16, "Depth": 2, "Channels": 6, "Source": "Micro-Manager", "ChColors": [ -16711681, -256, -65281, -1, -1, -16776961 ], "MetadataVersion": 10, "KeepShutterOpenChannels": true, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536, 65536 ], "CustomIntervals_ms": [], "Height": 1200, "MicroManagerVersion": "1.4.23 20180219", "GridColumn": 0, "UUID": "3ad8801b-5699-40d6-813b-4ad840863e38", "PixelType": "GRAY16", "Width": 1200, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\Yan\\20191216-YC113-S11S12-miseq", "Frames": 1, "Comment": "", "UserName": "Nikon", "PixelSize_um": 1.1, "ROI": [ 0, 0, 1200, 1200 ], "Date": "2019-12-17", "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC", "P-DAPI-high" ], "KeepShutterOpenSlices": true, "IJType": 1, "ComputerName": "ZadorConfocal2", "Positions": 18, "PositionIndex": 17, "CameraTimeout": 20000, "TimeFirst": false }, "640 AO-Name": "AnalogIO", "Vincent-D3-Command": "Close" }, "FrameKey-0-0-7": { "COM4-StopBits": "1", "528 AO-MaxVolts": "5.0000", "XLIGHT Excitation Wheel-State": "1", "470 Shutter-Name": "AnalogIO", "Core-Shutter": "520", "555 AO-Demo": "No", "XLIGHT Dichroic-State": "2", "XYStage-StepSize-Y": "0.1000", "ZPositionUm": 223.215, "XYStage-StepSize-X": "0.1000", "PRIME-FirmwareVersion": "20.28", "PRIME-ClearCycles": "2", "520-Name": "DA Shutter", "405 Shutter-Name": "AnalogIO", "PIEZO-StepSize": "0.0050", "PRIME-ClearMode": "Pre-Exposure", "470 AO-MinVolts": "0.0000", "405 AO-SupportsTriggering": "1", "CameraChannelIndex": 0, "XYStage-StartSpeed": "500.0000", "COM9-StopBits": "2", "405 AO-Description": "NI DAC", "COM4-DelayBetweenCharsMs": "0.0000", "445 AO-Block voltage": "false", "520 AO-TriggerInputLine": "/Dev1/PFI0", "555 AO-Description": "NI DAC", "520 AO-TriggerSampleRate": "100000", "COM4-Verbose": "1", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM4-Name": "COM4", "405 Shutter-Volts": "0.0000", "Vincent-D3-Port": "COM1", "PRIME-PP 4 MIN ADU AFFECTED": "200", "470 Shutter-MinVolts": "0.0000", "PRIME-PP 3 ENABLED": "Yes", "470 AO-Description": "NI DAC", "PRIME-Description": "PVCAM API device adapter", "520 Shutter-MaxVolts": "5.0000", "520 Shutter-Volts": "5.0000", "528 AO-Sequenceable": "1", "PRIME-Binning": "1x1", "555 Shutter-MaxVolts": "5.0000", "528 Shutter-TriggerSequenceLength": "1000", "405 Shutter-Block voltage": "false", "XLIGHT Spin motor-State": "1", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "Vincent-D3-Time to open (ms)": "35.0000", "Vincent-D3-Time to close (ms)": "35.0000", "445 AO-TriggerSampleRate": "100000", "640 Shutter-IOChannel": "Dev1/ao14", "555 AO-Sequenceable": "1", "520 AO-Name": "AnalogIO", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "470 AO-Block voltage": "false", "COM1-Verbose": "1", "COM9-AnswerTimeout": "10000.0000", "528 Shutter-Name": "AnalogIO", "PRIME-Trigger Timeout (secs)": "10", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "NextFrame": 0, "470 AO-TriggerSequenceLength": "1000", "PRIME-TransposeXY": "0", "445 AO-TriggerSequenceLength": "1000", "COM1-Name": "COM1", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "COM9-DataBits": "8", "COM1-AnswerTimeout": "500.0000", "Camera": "", "TE2000-Version": "V2.50-V2.50-V2.10", "528 Shutter-Volts": "0.0000", "XLIGHT Hub-Name": "XLIGHT Hub", "PIEZO-Description": "Ludl stage driver adapter", "PositionName": "Pos17", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSampleRate": "100000", "PRIME-FanSpeedSetpoint": "High", "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "405 AO-TriggerSampleRate": "100000", "470 Shutter-Block voltage": "false", "470-State": "0", "COM3-Parity": "None", "405 AO-Volts": "0.0000", "PRIME-TransposeMirrorY": "0", "PRIME-ChipName": "GS144BSI", "470 AO-Volts": "0.0000", "PRIME-TransposeMirrorX": "0", "445 Shutter-SupportsTriggering": "0", "470 Shutter-SupportsTriggering": "0", "XLIGHT Hub-Description": "XLIGHT hub", "PRIME-PreampOffLimit": "10000", "470 AO-Sequenceable": "1", "528 Shutter-MaxVolts": "5.0000", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "Core-XYStage": "XYStage", "470 AO-TriggerSampleRate": "100000", "PRIME-Timing-PreTriggerDelayNs": "24672000.0000", "528-DA Device": "528 Shutter", "Focus-Description": "Nikon TE2000 focus adapter", "FilterBlock-State": "5", "520 AO-Demo": "No", "COM3-DelayBetweenCharsMs": "0.0000", "470 Shutter-Volts": "0.0000", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "PRIME-PP 2 THRESHOLD": "125", "PRIME-PP 4 THRESHOLD": "75", "XYStage-Acceleration": "4.0000", "470 Shutter-Demo": "No", "PRIME-Name": "PMPCIECam00", "640-DA Device": "640 Shutter", "405 AO-Block voltage": "false", "ElapsedTime-ms": 720258, "PRIME-Offset": "100", "445 AO-IOChannel": "Dev1/ao1", "640 AO-MaxVolts": "5.0000", "PositionIndex": 17, "PRIME-PP 1 ENABLED": "Yes", "COM1-BaudRate": "9600", "640-State": "0", "COM3-Verbose": "1", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "LudlController-TransmissionDelay": "0", "PRIME-FullWellCapacity": "62000", "PRIME-SMARTStreamingEnabled": "No", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "640 Shutter-Description": "NI DAC", "528-Description": "DA device that is used as a shutter", "COM4-Description": "Serial port driver (boost:asio)", "405 Shutter-Description": "NI DAC", "TE2000-Name": "TE2000", "528 Shutter-Description": "NI DAC", "COM9-Description": "Serial port driver (boost:asio)", "LudlController-Name": "LudlController", "445 Shutter-TriggerSequenceLength": "1000", "PRIME-SerialNumber": "A18B203008", "445 AO-Volts": "0.0000", "PRIME-X-dimension": "1200", "PRIME-ShutterCloseDelay": "0", "XLIGHT Disk slider-State": "2", "Position": "Default", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 AO-IOChannel": "Dev1/ao5", "520 AO-IOChannel": "Dev1/ao4", "528 AO-TriggerSequenceLength": "1000", "405 Shutter-MaxVolts": "5.0000", "470-DA Device": "470 Shutter", "PRIME-FTCapable": "No", "Core-ChannelGroup": "Channels", "555 AO-SupportsTriggering": "0", "PRIME-Y-dimension": "1200", "Frame": 0, "555 Shutter-Sequenceable": "1", "PRIME-Timing-ExposureTimeNs": "499998000.0000", "Core-ImageProcessor": "", "528-State": "0", "520 AO-Block voltage": "false", "XLIGHT Excitation Wheel-Label": "CFP/YFP", "640 Shutter-Demo": "No", "COM3-StopBits": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "520 Shutter-IOChannel": "Dev1/ao11", "Width": 1200, "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-SupportsTriggering": "0", "640 AO-TriggerSampleRate": "100000", "520-Description": "DA device that is used as a shutter", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "405 Shutter-Sequenceable": "1", "405 Shutter-SupportsTriggering": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-TriggerSampleRate": "100000", "640 Shutter-MaxVolts": "5.0000", "555 AO-MaxVolts": "5.0000", "520 AO-TriggerSequenceLength": "1000", "COM3-BaudRate": "9600", "COM9-BaudRate": "9600", "Vincent-D3-Channel #": "Ch. #1", "PRIME-PMode": "Normal", "OpticalPath-Label": "Left(100)", "BitDepth": 16, "555 AO-TriggerSequenceLength": "1000", "445 Shutter-Description": "NI DAC", "405 AO-Sequenceable": "1", "PRIME-AcquisitionMethod": "Callbacks", "555 AO-Name": "AnalogIO", "PRIME-ShutterMode": "Pre-Exposure", "COM3-Handshaking": "Off", "445 AO-SupportsTriggering": "0", "ROI": "0-0-1200-1200", "COM9-Verbose": "1", "PRIME-Color": "OFF", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "PRIME-ShutterOpenDelay": "0", "COM1-Parity": "None", "Slice": 7, "SlicePosition": 20, "COM9-DelayBetweenCharsMs": "0.0000", "555-Name": "DA Shutter", "PIEZO-LudlSingleAxisName": "B", "XLIGHT Emission Wheel-Label": "565/24(G)", "640 Shutter-Name": "AnalogIO", "640 AO-Sequenceable": "1", "520 Shutter-Demo": "No", "528 Shutter-Demo": "No", "470-Name": "DA Shutter", "520-DA Device": "520 Shutter", "XYStage-Description": "Ludl XY stage driver adapter", "445 Shutter-MinVolts": "0.0000", "528 AO-TriggerSampleRate": "100000", "405 AO-MinVolts": "0.0000", "405 Shutter-TriggerSequenceLength": "1000", "Height": 1200, "555 AO-Block voltage": "false", "640 Shutter-Volts": "0.0000", "PixelType": "GRAY16", "445 Shutter-Block voltage": "false", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "520 Shutter-TriggerSequenceLength": "1000", "COM9-Name": "COM9", "Core-SLM": "", "555 AO-Volts": "0.0000", "555 Shutter-Block voltage": "false", "XYStage-Speed": "1800.0000", "520 AO-MaxVolts": "5.0000", "528 Shutter-SupportsTriggering": "0", "COM1-Description": "Serial port driver (boost:asio)", "PRIME-MetadataEnabled": "No", "520 Shutter-MinVolts": "0.0000", "470 Shutter-Sequenceable": "1", "PRIME-PreampDelay": "0", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-MinVolts": "0.0000", "ChannelIndex": 0, "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Core-AutoShutter": "1", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "Exposure-ms": 320, "PRIME-PP 2 ENABLED": "Yes", "640 Shutter-TriggerSampleRate": "100000", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "470 Shutter-IOChannel": "Dev1/ao10", "405 AO-Demo": "No", "PixelSizeUm": 1.1, "470 AO-Demo": "No", "470 Shutter-MaxVolts": "5.0000", "Vincent-D3-Name": "Vincent-D3", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FileName": "img_000000000_Il-G_007.tif", "LudlController-Reset": "Operate", "PRIME-Exposure": "320.00", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "445-Name": "DA Shutter", "555 Shutter-Name": "AnalogIO", "520 Shutter-TriggerSampleRate": "100000", "445 Shutter-IOChannel": "Dev1/ao9", "PRIME-TransposeCorrection": "0", "XYStage-StepSize": "0.1000", "445-Description": "DA device that is used as a shutter", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 AO-IOChannel": "Dev1/ao7", "520 Shutter-Name": "AnalogIO", "COM3-DataBits": "8", "640 AO-Volts": "0.0000", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "555 Shutter-IOChannel": "Dev1/ao13", "WaitInterval": null, "520 Shutter-Sequenceable": "1", "YPositionUm": -13012.300000000001, "445-State": "0", "445-DA Device": "445 Shutter", "PRIME-CircularBufferAutoSize": "ON", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "555 AO-TriggerSampleRate": "100000", "445 Shutter-Volts": "0.0000", "XLIGHT Hub-Port": "COM3", "Core-AutoFocus": "", "COM4-Handshaking": "Off", "528 Shutter-MinVolts": "0.0000", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "445 Shutter-Name": "AnalogIO", "528 Shutter-Sequenceable": "1", "XYStage-TransposeMirrorX": "0", "XYStage-TransposeMirrorY": "0", "PRIME-ReadoutRate": "100MHz 16bit", "XYStage-Name": "Stage", "640 AO-SupportsTriggering": "0", "640-Name": "DA Shutter", "640 Shutter-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "640 AO-Block voltage": "false", "445 Shutter-Sequenceable": "1", "470 AO-MaxVolts": "5.0000", "COM4-BaudRate": "9600", "470-Description": "DA device that is used as a shutter", "520 Shutter-Description": "NI DAC", "PRIME-CCDTemperature": "-14.9200", "Time": "2019-12-17 03:59:37 -0500", "640 AO-TriggerSequenceLength": "1000", "470 AO-Name": "AnalogIO", "XLIGHT Disk slider-Label": "Disk Pos 40um", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "Binning": "1x1", "TE2000-ModelType": "1", "COM3-AnswerTimeout": "500.0000", "555 Shutter-SupportsTriggering": "0", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "TE2000-Port": "COM4", "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Description": "NI DAC", "COM4-AnswerTimeout": "500.0000", "COM1-DelayBetweenCharsMs": "0.0000", "FilterBlock-Label": "Confocal", "445 AO-Name": "AnalogIO", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-Timing-ReadoutTimeNs": "24672000.0000", "528 AO-Demo": "No", "520 AO-Sequenceable": "1", "555-Description": "DA device that is used as a shutter", "405-DA Device": "405 Shutter", "405 AO-TriggerSequenceLength": "1000", "640 AO-MinVolts": "0.0000", "640 Shutter-Block voltage": "false", "FrameIndex": 0, "PRIME-Port": "CMOS", "555 AO-MinVolts": "0.0000", "COM3-Description": "Serial port driver (boost:asio)", "528 AO-Description": "NI DAC", "555 AO-IOChannel": "Dev1/ao6", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "555 AO-TriggerInputLine": "/Dev1/PFI0", "PRIME-ActualInterval-ms": "84.6200", "LudlController-Port": "COM9", "445 Shutter-Demo": "No", "PRIME-Trigger-Expose Out-Mux": "1", "SliceIndex": 7, "PRIME-CCDTemperatureSetPoint": "-15.0000", "405 Shutter-MinVolts": "0.0000", "PRIME-ExposeOutMode": "First Row", "AxisPositions": null, "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Volts": "0.0000", "528-Name": "DA Shutter", "555-State": "0", "XLIGHT Dichroic-Label": "CFP/YFP", "445 AO-Sequenceable": "1", "405-Name": "DA Shutter", "405 AO-IOChannel": "Dev1/ao0", "Core-Camera": "PRIME", "Focus-Name": "Focus", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "405 Shutter-IOChannel": "Dev1/ao8", "640 AO-Demo": "No", "640 Shutter-SupportsTriggering": "0", "445 AO-Demo": "No", "555-DA Device": "555 Shutter", "470 Shutter-Description": "NI DAC", "528 AO-Volts": "0.0000", "COM1-DataBits": "8", "Vincent-D3-Address": "x", "520 AO-SupportsTriggering": "0", "405-Description": "DA device that is used as a shutter", "640 Shutter-MinVolts": "0.0000", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "405 AO-Name": "AnalogIO", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "640 AO-TriggerInputLine": "/Dev1/PFI0", "405 AO-TriggerInputLine": "/Dev1/PFI0", "640-Description": "DA device that is used as a shutter", "445 Shutter-TriggerSampleRate": "100000", "470 Shutter-TriggerSequenceLength": "1000", "COM1-StopBits": "1", "520 Shutter-SupportsTriggering": "0", "405-State": "0", "Core-Initialize": "1", "XLIGHT Emission Wheel-State": "3", "520 AO-Description": "NI DAC", "528 AO-TriggerInputLine": "/Dev1/PFI0", "445 Shutter-MaxVolts": "5.0000", "COM9-Handshaking": "Off", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "XLIGHT Spin motor-Label": "On (spinning)", "PRIME-PP 5 ENABLED": "No", "UUID": "1ff1ed3b-4196-47e7-94d8-9bd8b2774442", "PRIME-TriggerMode": "Internal Trigger", "COM1-Handshaking": "Off", "PRIME-Timing-PostTriggerDelayNs": "49344000.0000", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "Focus-Position": "16732.9000", "528 Shutter-Block voltage": "false", "Channel": "Il-G", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "555 Shutter-Demo": "No", "405 Shutter-Demo": "No", "528 AO-Name": "AnalogIO", "520-State": "1", "Core-Galvo": "", "405 Shutter-TriggerSampleRate": "100000", "Focus-FocusStepSize_(nm)": "100", "PRIME-PixelType": "16bit", "COM4-DataBits": "8", "470 AO-IOChannel": "Dev1/ao2", "520 AO-MinVolts": "0.0000", "COM9-Parity": "None", "PRIME-CircularBufferEnabled": "ON", "PRIME-PP 6 Reset": "No", "640 AO-Description": "NI DAC", "Source": "PRIME", "445 AO-MinVolts": "0.0000", "PIEZO-Name": "Stage", "445 AO-Description": "NI DAC", "PRIME-CircularBufferFrameCount": "46", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "520 AO-Volts": "4.5000", "528 Shutter-TriggerSampleRate": "100000", "XPositionUm": 23269.100000000002, "OpticalPath-State": "4", "PRIME-PP 3 THRESHOLD": "80", "PRIME-PVCAM Version": "3.7.1", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Gain": "1-HDR", "640 Shutter-TriggerSequenceLength": "1000", "528 AO-SupportsTriggering": "0", "445 AO-TriggerInputLine": "/Dev1/PFI0", "OpticalPath-Name": "OpticalPath", "PRIME-PP 4 ENABLED": "Yes", "PRIME-PVCAM Adapter Version": "1.3.29", "COM3-Name": "COM3", "405 AO-MaxVolts": "5.0000", "520 Shutter-Block voltage": "false", "PRIME-Timing-ClearingTimeNs": "49344000.0000", "Core-Focus": "PIEZO", "528 AO-Block voltage": "false", "TE2000-Description": "Nikon TE2000 hub", "528 AO-MinVolts": "0.0000", "COM4-Parity": "None", "Summary": { "Slices": 11, "ChContrastMin": [ 0, 0, 0, 0, 0, 0 ], "PixelAspect": 1, "SlicesFirst": true, "Interval_ms": 0, "Time": "2019-12-17 03:59:33 -0500", "Prefix": "seq_7", "InitialPositionList": [ { "Label": "Pos0", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [149.91], "Focus": [16657.7], "XYStage": [ 71629.7, 19152.9 ] }, "GridColumnIndex": 0 }, { "Label": "Pos1", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [190.82], "Focus": [16485.4], "XYStage": [ 65702.3, 19396.600000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos2", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [194.69], "Focus": [16446.8], "XYStage": [ 59880.5, 19007.8 ] }, "GridColumnIndex": 0 }, { "Label": "Pos3", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [145.04], "Focus": [16446.6], "XYStage": [ 53616.9, 18818.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos4", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [169.57500000000002], "Focus": [16446.5], "XYStage": [ 47662.8, 18817.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos5", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [131.805], "Focus": [16446.4], "XYStage": [ 41918.8, 18298.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos6", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [113.145], "Focus": [16446.5], "XYStage": [ 36129.9, 18895.8 ] }, "GridColumnIndex": 0 }, { "Label": "Pos7", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [141.005], "Focus": [16446.6], "XYStage": [ 30299.100000000002, 18895.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos8", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [140.97], "Focus": [16446.5], "XYStage": [ 23952.100000000002, 19156 ] }, "GridColumnIndex": 0 }, { "Label": "Pos9", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [184.57500000000002], "Focus": [16677.9], "XYStage": [ 72866.8, -14809.300000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos10", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [167.95000000000002], "Focus": [16557.9], "XYStage": [ 66515.6, -14371.300000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos11", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [168], "Focus": [16618.9], "XYStage": [ 59724.3, -13964.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos12", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [203.205], "Focus": [16779.6], "XYStage": [ 53862.3, -13494.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos13", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [203.20000000000002], "Focus": [16749], "XYStage": [ 48108.3, -13805.400000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos14", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [203.19], "Focus": [16675.9], "XYStage": [ 41645, -13804.900000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos15", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [203.21], "Focus": [16675.7], "XYStage": [ 35352.6, -13805.6 ] }, "GridColumnIndex": 0 }, { "Label": "Pos16", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [203.225], "Focus": [16675.8], "XYStage": [ 29298, -13447.400000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos17", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [203.215], "Focus": [16675.8], "XYStage": [ 23269.100000000002, -13012.300000000001 ] }, "GridColumnIndex": 0 } ], "z-step_um": 10, "GridRow": 0, "BitDepth": 16, "Depth": 2, "Channels": 6, "Source": "Micro-Manager", "ChColors": [ -16711681, -256, -65281, -1, -1, -16776961 ], "MetadataVersion": 10, "KeepShutterOpenChannels": true, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536, 65536 ], "CustomIntervals_ms": [], "Height": 1200, "MicroManagerVersion": "1.4.23 20180219", "GridColumn": 0, "UUID": "3ad8801b-5699-40d6-813b-4ad840863e38", "PixelType": "GRAY16", "Width": 1200, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\Yan\\20191216-YC113-S11S12-miseq", "Frames": 1, "Comment": "", "UserName": "Nikon", "PixelSize_um": 1.1, "ROI": [ 0, 0, 1200, 1200 ], "Date": "2019-12-17", "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC", "P-DAPI-high" ], "KeepShutterOpenSlices": true, "IJType": 1, "ComputerName": "ZadorConfocal2", "Positions": 18, "PositionIndex": 17, "CameraTimeout": 20000, "TimeFirst": false }, "640 AO-Name": "AnalogIO", "Vincent-D3-Command": "Close" }, "FrameKey-0-0-8": { "COM4-StopBits": "1", "528 AO-MaxVolts": "5.0000", "XLIGHT Excitation Wheel-State": "1", "470 Shutter-Name": "AnalogIO", "Core-Shutter": "520", "555 AO-Demo": "No", "XLIGHT Dichroic-State": "2", "XYStage-StepSize-Y": "0.1000", "ZPositionUm": 233.215, "XYStage-StepSize-X": "0.1000", "PRIME-FirmwareVersion": "20.28", "PRIME-ClearCycles": "2", "520-Name": "DA Shutter", "405 Shutter-Name": "AnalogIO", "PIEZO-StepSize": "0.0050", "PRIME-ClearMode": "Pre-Exposure", "470 AO-MinVolts": "0.0000", "405 AO-SupportsTriggering": "1", "CameraChannelIndex": 0, "XYStage-StartSpeed": "500.0000", "COM9-StopBits": "2", "405 AO-Description": "NI DAC", "COM4-DelayBetweenCharsMs": "0.0000", "445 AO-Block voltage": "false", "520 AO-TriggerInputLine": "/Dev1/PFI0", "555 AO-Description": "NI DAC", "520 AO-TriggerSampleRate": "100000", "COM4-Verbose": "1", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM4-Name": "COM4", "405 Shutter-Volts": "0.0000", "Vincent-D3-Port": "COM1", "PRIME-PP 4 MIN ADU AFFECTED": "200", "470 Shutter-MinVolts": "0.0000", "PRIME-PP 3 ENABLED": "Yes", "470 AO-Description": "NI DAC", "PRIME-Description": "PVCAM API device adapter", "520 Shutter-MaxVolts": "5.0000", "520 Shutter-Volts": "5.0000", "528 AO-Sequenceable": "1", "PRIME-Binning": "1x1", "555 Shutter-MaxVolts": "5.0000", "528 Shutter-TriggerSequenceLength": "1000", "405 Shutter-Block voltage": "false", "XLIGHT Spin motor-State": "1", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "Vincent-D3-Time to open (ms)": "35.0000", "Vincent-D3-Time to close (ms)": "35.0000", "445 AO-TriggerSampleRate": "100000", "640 Shutter-IOChannel": "Dev1/ao14", "555 AO-Sequenceable": "1", "520 AO-Name": "AnalogIO", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "470 AO-Block voltage": "false", "COM1-Verbose": "1", "COM9-AnswerTimeout": "10000.0000", "528 Shutter-Name": "AnalogIO", "PRIME-Trigger Timeout (secs)": "10", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "NextFrame": 0, "470 AO-TriggerSequenceLength": "1000", "PRIME-TransposeXY": "0", "445 AO-TriggerSequenceLength": "1000", "COM1-Name": "COM1", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "COM9-DataBits": "8", "COM1-AnswerTimeout": "500.0000", "Camera": "", "TE2000-Version": "V2.50-V2.50-V2.10", "528 Shutter-Volts": "0.0000", "XLIGHT Hub-Name": "XLIGHT Hub", "PIEZO-Description": "Ludl stage driver adapter", "PositionName": "Pos17", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSampleRate": "100000", "PRIME-FanSpeedSetpoint": "High", "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "405 AO-TriggerSampleRate": "100000", "470 Shutter-Block voltage": "false", "470-State": "0", "COM3-Parity": "None", "405 AO-Volts": "0.0000", "PRIME-TransposeMirrorY": "0", "PRIME-ChipName": "GS144BSI", "470 AO-Volts": "0.0000", "PRIME-TransposeMirrorX": "0", "445 Shutter-SupportsTriggering": "0", "470 Shutter-SupportsTriggering": "0", "XLIGHT Hub-Description": "XLIGHT hub", "PRIME-PreampOffLimit": "10000", "470 AO-Sequenceable": "1", "528 Shutter-MaxVolts": "5.0000", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "Core-XYStage": "XYStage", "470 AO-TriggerSampleRate": "100000", "PRIME-Timing-PreTriggerDelayNs": "24672000.0000", "528-DA Device": "528 Shutter", "Focus-Description": "Nikon TE2000 focus adapter", "FilterBlock-State": "5", "520 AO-Demo": "No", "COM3-DelayBetweenCharsMs": "0.0000", "470 Shutter-Volts": "0.0000", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "PRIME-PP 2 THRESHOLD": "125", "PRIME-PP 4 THRESHOLD": "75", "XYStage-Acceleration": "4.0000", "470 Shutter-Demo": "No", "PRIME-Name": "PMPCIECam00", "640-DA Device": "640 Shutter", "405 AO-Block voltage": "false", "ElapsedTime-ms": 720833, "PRIME-Offset": "100", "445 AO-IOChannel": "Dev1/ao1", "640 AO-MaxVolts": "5.0000", "PositionIndex": 17, "PRIME-PP 1 ENABLED": "Yes", "COM1-BaudRate": "9600", "640-State": "0", "COM3-Verbose": "1", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "LudlController-TransmissionDelay": "0", "PRIME-FullWellCapacity": "62000", "PRIME-SMARTStreamingEnabled": "No", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "640 Shutter-Description": "NI DAC", "528-Description": "DA device that is used as a shutter", "COM4-Description": "Serial port driver (boost:asio)", "405 Shutter-Description": "NI DAC", "TE2000-Name": "TE2000", "528 Shutter-Description": "NI DAC", "COM9-Description": "Serial port driver (boost:asio)", "LudlController-Name": "LudlController", "445 Shutter-TriggerSequenceLength": "1000", "PRIME-SerialNumber": "A18B203008", "445 AO-Volts": "0.0000", "PRIME-X-dimension": "1200", "PRIME-ShutterCloseDelay": "0", "XLIGHT Disk slider-State": "2", "Position": "Default", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 AO-IOChannel": "Dev1/ao5", "520 AO-IOChannel": "Dev1/ao4", "528 AO-TriggerSequenceLength": "1000", "405 Shutter-MaxVolts": "5.0000", "470-DA Device": "470 Shutter", "PRIME-FTCapable": "No", "Core-ChannelGroup": "Channels", "555 AO-SupportsTriggering": "0", "PRIME-Y-dimension": "1200", "Frame": 0, "555 Shutter-Sequenceable": "1", "PRIME-Timing-ExposureTimeNs": "499998000.0000", "Core-ImageProcessor": "", "528-State": "0", "520 AO-Block voltage": "false", "XLIGHT Excitation Wheel-Label": "CFP/YFP", "640 Shutter-Demo": "No", "COM3-StopBits": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "520 Shutter-IOChannel": "Dev1/ao11", "Width": 1200, "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-SupportsTriggering": "0", "640 AO-TriggerSampleRate": "100000", "520-Description": "DA device that is used as a shutter", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "405 Shutter-Sequenceable": "1", "405 Shutter-SupportsTriggering": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-TriggerSampleRate": "100000", "640 Shutter-MaxVolts": "5.0000", "555 AO-MaxVolts": "5.0000", "520 AO-TriggerSequenceLength": "1000", "COM3-BaudRate": "9600", "COM9-BaudRate": "9600", "Vincent-D3-Channel #": "Ch. #1", "PRIME-PMode": "Normal", "OpticalPath-Label": "Left(100)", "BitDepth": 16, "555 AO-TriggerSequenceLength": "1000", "445 Shutter-Description": "NI DAC", "405 AO-Sequenceable": "1", "PRIME-AcquisitionMethod": "Callbacks", "555 AO-Name": "AnalogIO", "PRIME-ShutterMode": "Pre-Exposure", "COM3-Handshaking": "Off", "445 AO-SupportsTriggering": "0", "ROI": "0-0-1200-1200", "COM9-Verbose": "1", "PRIME-Color": "OFF", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "PRIME-ShutterOpenDelay": "0", "COM1-Parity": "None", "Slice": 8, "SlicePosition": 30, "COM9-DelayBetweenCharsMs": "0.0000", "555-Name": "DA Shutter", "PIEZO-LudlSingleAxisName": "B", "XLIGHT Emission Wheel-Label": "565/24(G)", "640 Shutter-Name": "AnalogIO", "640 AO-Sequenceable": "1", "520 Shutter-Demo": "No", "528 Shutter-Demo": "No", "470-Name": "DA Shutter", "520-DA Device": "520 Shutter", "XYStage-Description": "Ludl XY stage driver adapter", "445 Shutter-MinVolts": "0.0000", "528 AO-TriggerSampleRate": "100000", "405 AO-MinVolts": "0.0000", "405 Shutter-TriggerSequenceLength": "1000", "Height": 1200, "555 AO-Block voltage": "false", "640 Shutter-Volts": "0.0000", "PixelType": "GRAY16", "445 Shutter-Block voltage": "false", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "520 Shutter-TriggerSequenceLength": "1000", "COM9-Name": "COM9", "Core-SLM": "", "555 AO-Volts": "0.0000", "555 Shutter-Block voltage": "false", "XYStage-Speed": "1800.0000", "520 AO-MaxVolts": "5.0000", "528 Shutter-SupportsTriggering": "0", "COM1-Description": "Serial port driver (boost:asio)", "PRIME-MetadataEnabled": "No", "520 Shutter-MinVolts": "0.0000", "470 Shutter-Sequenceable": "1", "PRIME-PreampDelay": "0", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-MinVolts": "0.0000", "ChannelIndex": 0, "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Core-AutoShutter": "1", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "Exposure-ms": 320, "PRIME-PP 2 ENABLED": "Yes", "640 Shutter-TriggerSampleRate": "100000", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "470 Shutter-IOChannel": "Dev1/ao10", "405 AO-Demo": "No", "PixelSizeUm": 1.1, "470 AO-Demo": "No", "470 Shutter-MaxVolts": "5.0000", "Vincent-D3-Name": "Vincent-D3", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FileName": "img_000000000_Il-G_008.tif", "LudlController-Reset": "Operate", "PRIME-Exposure": "320.00", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "445-Name": "DA Shutter", "555 Shutter-Name": "AnalogIO", "520 Shutter-TriggerSampleRate": "100000", "445 Shutter-IOChannel": "Dev1/ao9", "PRIME-TransposeCorrection": "0", "XYStage-StepSize": "0.1000", "445-Description": "DA device that is used as a shutter", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 AO-IOChannel": "Dev1/ao7", "520 Shutter-Name": "AnalogIO", "COM3-DataBits": "8", "640 AO-Volts": "0.0000", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "555 Shutter-IOChannel": "Dev1/ao13", "WaitInterval": null, "520 Shutter-Sequenceable": "1", "YPositionUm": -13012.300000000001, "445-State": "0", "445-DA Device": "445 Shutter", "PRIME-CircularBufferAutoSize": "ON", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "555 AO-TriggerSampleRate": "100000", "445 Shutter-Volts": "0.0000", "XLIGHT Hub-Port": "COM3", "Core-AutoFocus": "", "COM4-Handshaking": "Off", "528 Shutter-MinVolts": "0.0000", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "445 Shutter-Name": "AnalogIO", "528 Shutter-Sequenceable": "1", "XYStage-TransposeMirrorX": "0", "XYStage-TransposeMirrorY": "0", "PRIME-ReadoutRate": "100MHz 16bit", "XYStage-Name": "Stage", "640 AO-SupportsTriggering": "0", "640-Name": "DA Shutter", "640 Shutter-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "640 AO-Block voltage": "false", "445 Shutter-Sequenceable": "1", "470 AO-MaxVolts": "5.0000", "COM4-BaudRate": "9600", "470-Description": "DA device that is used as a shutter", "520 Shutter-Description": "NI DAC", "PRIME-CCDTemperature": "-14.9200", "Time": "2019-12-17 03:59:37 -0500", "640 AO-TriggerSequenceLength": "1000", "470 AO-Name": "AnalogIO", "XLIGHT Disk slider-Label": "Disk Pos 40um", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "Binning": "1x1", "TE2000-ModelType": "1", "COM3-AnswerTimeout": "500.0000", "555 Shutter-SupportsTriggering": "0", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "TE2000-Port": "COM4", "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Description": "NI DAC", "COM4-AnswerTimeout": "500.0000", "COM1-DelayBetweenCharsMs": "0.0000", "FilterBlock-Label": "Confocal", "445 AO-Name": "AnalogIO", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-Timing-ReadoutTimeNs": "24672000.0000", "528 AO-Demo": "No", "520 AO-Sequenceable": "1", "555-Description": "DA device that is used as a shutter", "405-DA Device": "405 Shutter", "405 AO-TriggerSequenceLength": "1000", "640 AO-MinVolts": "0.0000", "640 Shutter-Block voltage": "false", "FrameIndex": 0, "PRIME-Port": "CMOS", "555 AO-MinVolts": "0.0000", "COM3-Description": "Serial port driver (boost:asio)", "528 AO-Description": "NI DAC", "555 AO-IOChannel": "Dev1/ao6", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "555 AO-TriggerInputLine": "/Dev1/PFI0", "PRIME-ActualInterval-ms": "84.6200", "LudlController-Port": "COM9", "445 Shutter-Demo": "No", "PRIME-Trigger-Expose Out-Mux": "1", "SliceIndex": 8, "PRIME-CCDTemperatureSetPoint": "-15.0000", "405 Shutter-MinVolts": "0.0000", "PRIME-ExposeOutMode": "First Row", "AxisPositions": null, "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Volts": "0.0000", "528-Name": "DA Shutter", "555-State": "0", "XLIGHT Dichroic-Label": "CFP/YFP", "445 AO-Sequenceable": "1", "405-Name": "DA Shutter", "405 AO-IOChannel": "Dev1/ao0", "Core-Camera": "PRIME", "Focus-Name": "Focus", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "405 Shutter-IOChannel": "Dev1/ao8", "640 AO-Demo": "No", "640 Shutter-SupportsTriggering": "0", "445 AO-Demo": "No", "555-DA Device": "555 Shutter", "470 Shutter-Description": "NI DAC", "528 AO-Volts": "0.0000", "COM1-DataBits": "8", "Vincent-D3-Address": "x", "520 AO-SupportsTriggering": "0", "405-Description": "DA device that is used as a shutter", "640 Shutter-MinVolts": "0.0000", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "405 AO-Name": "AnalogIO", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "640 AO-TriggerInputLine": "/Dev1/PFI0", "405 AO-TriggerInputLine": "/Dev1/PFI0", "640-Description": "DA device that is used as a shutter", "445 Shutter-TriggerSampleRate": "100000", "470 Shutter-TriggerSequenceLength": "1000", "COM1-StopBits": "1", "520 Shutter-SupportsTriggering": "0", "405-State": "0", "Core-Initialize": "1", "XLIGHT Emission Wheel-State": "3", "520 AO-Description": "NI DAC", "528 AO-TriggerInputLine": "/Dev1/PFI0", "445 Shutter-MaxVolts": "5.0000", "COM9-Handshaking": "Off", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "XLIGHT Spin motor-Label": "On (spinning)", "PRIME-PP 5 ENABLED": "No", "UUID": "be8f3013-9828-48ec-a37e-c53edf5bed86", "PRIME-TriggerMode": "Internal Trigger", "COM1-Handshaking": "Off", "PRIME-Timing-PostTriggerDelayNs": "49344000.0000", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "Focus-Position": "16732.9000", "528 Shutter-Block voltage": "false", "Channel": "Il-G", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "555 Shutter-Demo": "No", "405 Shutter-Demo": "No", "528 AO-Name": "AnalogIO", "520-State": "1", "Core-Galvo": "", "405 Shutter-TriggerSampleRate": "100000", "Focus-FocusStepSize_(nm)": "100", "PRIME-PixelType": "16bit", "COM4-DataBits": "8", "470 AO-IOChannel": "Dev1/ao2", "520 AO-MinVolts": "0.0000", "COM9-Parity": "None", "PRIME-CircularBufferEnabled": "ON", "PRIME-PP 6 Reset": "No", "640 AO-Description": "NI DAC", "Source": "PRIME", "445 AO-MinVolts": "0.0000", "PIEZO-Name": "Stage", "445 AO-Description": "NI DAC", "PRIME-CircularBufferFrameCount": "46", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "520 AO-Volts": "4.5000", "528 Shutter-TriggerSampleRate": "100000", "XPositionUm": 23269.100000000002, "OpticalPath-State": "4", "PRIME-PP 3 THRESHOLD": "80", "PRIME-PVCAM Version": "3.7.1", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Gain": "1-HDR", "640 Shutter-TriggerSequenceLength": "1000", "528 AO-SupportsTriggering": "0", "445 AO-TriggerInputLine": "/Dev1/PFI0", "OpticalPath-Name": "OpticalPath", "PRIME-PP 4 ENABLED": "Yes", "PRIME-PVCAM Adapter Version": "1.3.29", "COM3-Name": "COM3", "405 AO-MaxVolts": "5.0000", "520 Shutter-Block voltage": "false", "PRIME-Timing-ClearingTimeNs": "49344000.0000", "Core-Focus": "PIEZO", "528 AO-Block voltage": "false", "TE2000-Description": "Nikon TE2000 hub", "528 AO-MinVolts": "0.0000", "COM4-Parity": "None", "Summary": { "Slices": 11, "ChContrastMin": [ 0, 0, 0, 0, 0, 0 ], "PixelAspect": 1, "SlicesFirst": true, "Interval_ms": 0, "Time": "2019-12-17 03:59:33 -0500", "Prefix": "seq_7", "InitialPositionList": [ { "Label": "Pos0", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [149.91], "Focus": [16657.7], "XYStage": [ 71629.7, 19152.9 ] }, "GridColumnIndex": 0 }, { "Label": "Pos1", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [190.82], "Focus": [16485.4], "XYStage": [ 65702.3, 19396.600000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos2", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [194.69], "Focus": [16446.8], "XYStage": [ 59880.5, 19007.8 ] }, "GridColumnIndex": 0 }, { "Label": "Pos3", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [145.04], "Focus": [16446.6], "XYStage": [ 53616.9, 18818.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos4", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [169.57500000000002], "Focus": [16446.5], "XYStage": [ 47662.8, 18817.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos5", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [131.805], "Focus": [16446.4], "XYStage": [ 41918.8, 18298.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos6", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [113.145], "Focus": [16446.5], "XYStage": [ 36129.9, 18895.8 ] }, "GridColumnIndex": 0 }, { "Label": "Pos7", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [141.005], "Focus": [16446.6], "XYStage": [ 30299.100000000002, 18895.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos8", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [140.97], "Focus": [16446.5], "XYStage": [ 23952.100000000002, 19156 ] }, "GridColumnIndex": 0 }, { "Label": "Pos9", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [184.57500000000002], "Focus": [16677.9], "XYStage": [ 72866.8, -14809.300000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos10", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [167.95000000000002], "Focus": [16557.9], "XYStage": [ 66515.6, -14371.300000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos11", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [168], "Focus": [16618.9], "XYStage": [ 59724.3, -13964.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos12", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [203.205], "Focus": [16779.6], "XYStage": [ 53862.3, -13494.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos13", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [203.20000000000002], "Focus": [16749], "XYStage": [ 48108.3, -13805.400000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos14", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [203.19], "Focus": [16675.9], "XYStage": [ 41645, -13804.900000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos15", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [203.21], "Focus": [16675.7], "XYStage": [ 35352.6, -13805.6 ] }, "GridColumnIndex": 0 }, { "Label": "Pos16", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [203.225], "Focus": [16675.8], "XYStage": [ 29298, -13447.400000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos17", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [203.215], "Focus": [16675.8], "XYStage": [ 23269.100000000002, -13012.300000000001 ] }, "GridColumnIndex": 0 } ], "z-step_um": 10, "GridRow": 0, "BitDepth": 16, "Depth": 2, "Channels": 6, "Source": "Micro-Manager", "ChColors": [ -16711681, -256, -65281, -1, -1, -16776961 ], "MetadataVersion": 10, "KeepShutterOpenChannels": true, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536, 65536 ], "CustomIntervals_ms": [], "Height": 1200, "MicroManagerVersion": "1.4.23 20180219", "GridColumn": 0, "UUID": "3ad8801b-5699-40d6-813b-4ad840863e38", "PixelType": "GRAY16", "Width": 1200, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\Yan\\20191216-YC113-S11S12-miseq", "Frames": 1, "Comment": "", "UserName": "Nikon", "PixelSize_um": 1.1, "ROI": [ 0, 0, 1200, 1200 ], "Date": "2019-12-17", "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC", "P-DAPI-high" ], "KeepShutterOpenSlices": true, "IJType": 1, "ComputerName": "ZadorConfocal2", "Positions": 18, "PositionIndex": 17, "CameraTimeout": 20000, "TimeFirst": false }, "640 AO-Name": "AnalogIO", "Vincent-D3-Command": "Close" }, "FrameKey-0-0-9": { "COM4-StopBits": "1", "528 AO-MaxVolts": "5.0000", "XLIGHT Excitation Wheel-State": "1", "470 Shutter-Name": "AnalogIO", "Core-Shutter": "520", "555 AO-Demo": "No", "XLIGHT Dichroic-State": "2", "XYStage-StepSize-Y": "0.1000", "ZPositionUm": 243.215, "XYStage-StepSize-X": "0.1000", "PRIME-FirmwareVersion": "20.28", "PRIME-ClearCycles": "2", "520-Name": "DA Shutter", "405 Shutter-Name": "AnalogIO", "PIEZO-StepSize": "0.0050", "PRIME-ClearMode": "Pre-Exposure", "470 AO-MinVolts": "0.0000", "405 AO-SupportsTriggering": "1", "CameraChannelIndex": 0, "XYStage-StartSpeed": "500.0000", "COM9-StopBits": "2", "405 AO-Description": "NI DAC", "COM4-DelayBetweenCharsMs": "0.0000", "445 AO-Block voltage": "false", "520 AO-TriggerInputLine": "/Dev1/PFI0", "555 AO-Description": "NI DAC", "520 AO-TriggerSampleRate": "100000", "COM4-Verbose": "1", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM4-Name": "COM4", "405 Shutter-Volts": "0.0000", "Vincent-D3-Port": "COM1", "PRIME-PP 4 MIN ADU AFFECTED": "200", "470 Shutter-MinVolts": "0.0000", "PRIME-PP 3 ENABLED": "Yes", "470 AO-Description": "NI DAC", "PRIME-Description": "PVCAM API device adapter", "520 Shutter-MaxVolts": "5.0000", "520 Shutter-Volts": "5.0000", "528 AO-Sequenceable": "1", "PRIME-Binning": "1x1", "555 Shutter-MaxVolts": "5.0000", "528 Shutter-TriggerSequenceLength": "1000", "405 Shutter-Block voltage": "false", "XLIGHT Spin motor-State": "1", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "Vincent-D3-Time to open (ms)": "35.0000", "Vincent-D3-Time to close (ms)": "35.0000", "445 AO-TriggerSampleRate": "100000", "640 Shutter-IOChannel": "Dev1/ao14", "555 AO-Sequenceable": "1", "520 AO-Name": "AnalogIO", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "470 AO-Block voltage": "false", "COM1-Verbose": "1", "COM9-AnswerTimeout": "10000.0000", "528 Shutter-Name": "AnalogIO", "PRIME-Trigger Timeout (secs)": "10", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "NextFrame": 0, "470 AO-TriggerSequenceLength": "1000", "PRIME-TransposeXY": "0", "445 AO-TriggerSequenceLength": "1000", "COM1-Name": "COM1", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "COM9-DataBits": "8", "COM1-AnswerTimeout": "500.0000", "Camera": "", "TE2000-Version": "V2.50-V2.50-V2.10", "528 Shutter-Volts": "0.0000", "XLIGHT Hub-Name": "XLIGHT Hub", "PIEZO-Description": "Ludl stage driver adapter", "PositionName": "Pos17", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSampleRate": "100000", "PRIME-FanSpeedSetpoint": "High", "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "405 AO-TriggerSampleRate": "100000", "470 Shutter-Block voltage": "false", "470-State": "0", "COM3-Parity": "None", "405 AO-Volts": "0.0000", "PRIME-TransposeMirrorY": "0", "PRIME-ChipName": "GS144BSI", "470 AO-Volts": "0.0000", "PRIME-TransposeMirrorX": "0", "445 Shutter-SupportsTriggering": "0", "470 Shutter-SupportsTriggering": "0", "XLIGHT Hub-Description": "XLIGHT hub", "PRIME-PreampOffLimit": "10000", "470 AO-Sequenceable": "1", "528 Shutter-MaxVolts": "5.0000", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "Core-XYStage": "XYStage", "470 AO-TriggerSampleRate": "100000", "PRIME-Timing-PreTriggerDelayNs": "24672000.0000", "528-DA Device": "528 Shutter", "Focus-Description": "Nikon TE2000 focus adapter", "FilterBlock-State": "5", "520 AO-Demo": "No", "COM3-DelayBetweenCharsMs": "0.0000", "470 Shutter-Volts": "0.0000", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "PRIME-PP 2 THRESHOLD": "125", "PRIME-PP 4 THRESHOLD": "75", "XYStage-Acceleration": "4.0000", "470 Shutter-Demo": "No", "PRIME-Name": "PMPCIECam00", "640-DA Device": "640 Shutter", "405 AO-Block voltage": "false", "ElapsedTime-ms": 721392, "PRIME-Offset": "100", "445 AO-IOChannel": "Dev1/ao1", "640 AO-MaxVolts": "5.0000", "PositionIndex": 17, "PRIME-PP 1 ENABLED": "Yes", "COM1-BaudRate": "9600", "640-State": "0", "COM3-Verbose": "1", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "LudlController-TransmissionDelay": "0", "PRIME-FullWellCapacity": "62000", "PRIME-SMARTStreamingEnabled": "No", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "640 Shutter-Description": "NI DAC", "528-Description": "DA device that is used as a shutter", "COM4-Description": "Serial port driver (boost:asio)", "405 Shutter-Description": "NI DAC", "TE2000-Name": "TE2000", "528 Shutter-Description": "NI DAC", "COM9-Description": "Serial port driver (boost:asio)", "LudlController-Name": "LudlController", "445 Shutter-TriggerSequenceLength": "1000", "PRIME-SerialNumber": "A18B203008", "445 AO-Volts": "0.0000", "PRIME-X-dimension": "1200", "PRIME-ShutterCloseDelay": "0", "XLIGHT Disk slider-State": "2", "Position": "Default", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 AO-IOChannel": "Dev1/ao5", "520 AO-IOChannel": "Dev1/ao4", "528 AO-TriggerSequenceLength": "1000", "405 Shutter-MaxVolts": "5.0000", "470-DA Device": "470 Shutter", "PRIME-FTCapable": "No", "Core-ChannelGroup": "Channels", "555 AO-SupportsTriggering": "0", "PRIME-Y-dimension": "1200", "Frame": 0, "555 Shutter-Sequenceable": "1", "PRIME-Timing-ExposureTimeNs": "499998000.0000", "Core-ImageProcessor": "", "528-State": "0", "520 AO-Block voltage": "false", "XLIGHT Excitation Wheel-Label": "CFP/YFP", "640 Shutter-Demo": "No", "COM3-StopBits": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "520 Shutter-IOChannel": "Dev1/ao11", "Width": 1200, "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-SupportsTriggering": "0", "640 AO-TriggerSampleRate": "100000", "520-Description": "DA device that is used as a shutter", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "405 Shutter-Sequenceable": "1", "405 Shutter-SupportsTriggering": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-TriggerSampleRate": "100000", "640 Shutter-MaxVolts": "5.0000", "555 AO-MaxVolts": "5.0000", "520 AO-TriggerSequenceLength": "1000", "COM3-BaudRate": "9600", "COM9-BaudRate": "9600", "Vincent-D3-Channel #": "Ch. #1", "PRIME-PMode": "Normal", "OpticalPath-Label": "Left(100)", "BitDepth": 16, "555 AO-TriggerSequenceLength": "1000", "445 Shutter-Description": "NI DAC", "405 AO-Sequenceable": "1", "PRIME-AcquisitionMethod": "Callbacks", "555 AO-Name": "AnalogIO", "PRIME-ShutterMode": "Pre-Exposure", "COM3-Handshaking": "Off", "445 AO-SupportsTriggering": "0", "ROI": "0-0-1200-1200", "COM9-Verbose": "1", "PRIME-Color": "OFF", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "PRIME-ShutterOpenDelay": "0", "COM1-Parity": "None", "Slice": 9, "SlicePosition": 40, "COM9-DelayBetweenCharsMs": "0.0000", "555-Name": "DA Shutter", "PIEZO-LudlSingleAxisName": "B", "XLIGHT Emission Wheel-Label": "565/24(G)", "640 Shutter-Name": "AnalogIO", "640 AO-Sequenceable": "1", "520 Shutter-Demo": "No", "528 Shutter-Demo": "No", "470-Name": "DA Shutter", "520-DA Device": "520 Shutter", "XYStage-Description": "Ludl XY stage driver adapter", "445 Shutter-MinVolts": "0.0000", "528 AO-TriggerSampleRate": "100000", "405 AO-MinVolts": "0.0000", "405 Shutter-TriggerSequenceLength": "1000", "Height": 1200, "555 AO-Block voltage": "false", "640 Shutter-Volts": "0.0000", "PixelType": "GRAY16", "445 Shutter-Block voltage": "false", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "520 Shutter-TriggerSequenceLength": "1000", "COM9-Name": "COM9", "Core-SLM": "", "555 AO-Volts": "0.0000", "555 Shutter-Block voltage": "false", "XYStage-Speed": "1800.0000", "520 AO-MaxVolts": "5.0000", "528 Shutter-SupportsTriggering": "0", "COM1-Description": "Serial port driver (boost:asio)", "PRIME-MetadataEnabled": "No", "520 Shutter-MinVolts": "0.0000", "470 Shutter-Sequenceable": "1", "PRIME-PreampDelay": "0", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-MinVolts": "0.0000", "ChannelIndex": 0, "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Core-AutoShutter": "1", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "Exposure-ms": 320, "PRIME-PP 2 ENABLED": "Yes", "640 Shutter-TriggerSampleRate": "100000", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "470 Shutter-IOChannel": "Dev1/ao10", "405 AO-Demo": "No", "PixelSizeUm": 1.1, "470 AO-Demo": "No", "470 Shutter-MaxVolts": "5.0000", "Vincent-D3-Name": "Vincent-D3", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FileName": "img_000000000_Il-G_009.tif", "LudlController-Reset": "Operate", "PRIME-Exposure": "320.00", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "445-Name": "DA Shutter", "555 Shutter-Name": "AnalogIO", "520 Shutter-TriggerSampleRate": "100000", "445 Shutter-IOChannel": "Dev1/ao9", "PRIME-TransposeCorrection": "0", "XYStage-StepSize": "0.1000", "445-Description": "DA device that is used as a shutter", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 AO-IOChannel": "Dev1/ao7", "520 Shutter-Name": "AnalogIO", "COM3-DataBits": "8", "640 AO-Volts": "0.0000", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "555 Shutter-IOChannel": "Dev1/ao13", "WaitInterval": null, "520 Shutter-Sequenceable": "1", "YPositionUm": -13012.300000000001, "445-State": "0", "445-DA Device": "445 Shutter", "PRIME-CircularBufferAutoSize": "ON", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "555 AO-TriggerSampleRate": "100000", "445 Shutter-Volts": "0.0000", "XLIGHT Hub-Port": "COM3", "Core-AutoFocus": "", "COM4-Handshaking": "Off", "528 Shutter-MinVolts": "0.0000", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "445 Shutter-Name": "AnalogIO", "528 Shutter-Sequenceable": "1", "XYStage-TransposeMirrorX": "0", "XYStage-TransposeMirrorY": "0", "PRIME-ReadoutRate": "100MHz 16bit", "XYStage-Name": "Stage", "640 AO-SupportsTriggering": "0", "640-Name": "DA Shutter", "640 Shutter-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "640 AO-Block voltage": "false", "445 Shutter-Sequenceable": "1", "470 AO-MaxVolts": "5.0000", "COM4-BaudRate": "9600", "470-Description": "DA device that is used as a shutter", "520 Shutter-Description": "NI DAC", "PRIME-CCDTemperature": "-14.9200", "Time": "2019-12-17 03:59:38 -0500", "640 AO-TriggerSequenceLength": "1000", "470 AO-Name": "AnalogIO", "XLIGHT Disk slider-Label": "Disk Pos 40um", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "Binning": "1x1", "TE2000-ModelType": "1", "COM3-AnswerTimeout": "500.0000", "555 Shutter-SupportsTriggering": "0", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "TE2000-Port": "COM4", "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Description": "NI DAC", "COM4-AnswerTimeout": "500.0000", "COM1-DelayBetweenCharsMs": "0.0000", "FilterBlock-Label": "Confocal", "445 AO-Name": "AnalogIO", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-Timing-ReadoutTimeNs": "24672000.0000", "528 AO-Demo": "No", "520 AO-Sequenceable": "1", "555-Description": "DA device that is used as a shutter", "405-DA Device": "405 Shutter", "405 AO-TriggerSequenceLength": "1000", "640 AO-MinVolts": "0.0000", "640 Shutter-Block voltage": "false", "FrameIndex": 0, "PRIME-Port": "CMOS", "555 AO-MinVolts": "0.0000", "COM3-Description": "Serial port driver (boost:asio)", "528 AO-Description": "NI DAC", "555 AO-IOChannel": "Dev1/ao6", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "555 AO-TriggerInputLine": "/Dev1/PFI0", "PRIME-ActualInterval-ms": "84.6200", "LudlController-Port": "COM9", "445 Shutter-Demo": "No", "PRIME-Trigger-Expose Out-Mux": "1", "SliceIndex": 9, "PRIME-CCDTemperatureSetPoint": "-15.0000", "405 Shutter-MinVolts": "0.0000", "PRIME-ExposeOutMode": "First Row", "AxisPositions": null, "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Volts": "0.0000", "528-Name": "DA Shutter", "555-State": "0", "XLIGHT Dichroic-Label": "CFP/YFP", "445 AO-Sequenceable": "1", "405-Name": "DA Shutter", "405 AO-IOChannel": "Dev1/ao0", "Core-Camera": "PRIME", "Focus-Name": "Focus", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "405 Shutter-IOChannel": "Dev1/ao8", "640 AO-Demo": "No", "640 Shutter-SupportsTriggering": "0", "445 AO-Demo": "No", "555-DA Device": "555 Shutter", "470 Shutter-Description": "NI DAC", "528 AO-Volts": "0.0000", "COM1-DataBits": "8", "Vincent-D3-Address": "x", "520 AO-SupportsTriggering": "0", "405-Description": "DA device that is used as a shutter", "640 Shutter-MinVolts": "0.0000", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "405 AO-Name": "AnalogIO", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "640 AO-TriggerInputLine": "/Dev1/PFI0", "405 AO-TriggerInputLine": "/Dev1/PFI0", "640-Description": "DA device that is used as a shutter", "445 Shutter-TriggerSampleRate": "100000", "470 Shutter-TriggerSequenceLength": "1000", "COM1-StopBits": "1", "520 Shutter-SupportsTriggering": "0", "405-State": "0", "Core-Initialize": "1", "XLIGHT Emission Wheel-State": "3", "520 AO-Description": "NI DAC", "528 AO-TriggerInputLine": "/Dev1/PFI0", "445 Shutter-MaxVolts": "5.0000", "COM9-Handshaking": "Off", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "XLIGHT Spin motor-Label": "On (spinning)", "PRIME-PP 5 ENABLED": "No", "UUID": "9819c960-8647-4028-ad9d-63f1ab59c38d", "PRIME-TriggerMode": "Internal Trigger", "COM1-Handshaking": "Off", "PRIME-Timing-PostTriggerDelayNs": "49344000.0000", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "Focus-Position": "16732.9000", "528 Shutter-Block voltage": "false", "Channel": "Il-G", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "555 Shutter-Demo": "No", "405 Shutter-Demo": "No", "528 AO-Name": "AnalogIO", "520-State": "1", "Core-Galvo": "", "405 Shutter-TriggerSampleRate": "100000", "Focus-FocusStepSize_(nm)": "100", "PRIME-PixelType": "16bit", "COM4-DataBits": "8", "470 AO-IOChannel": "Dev1/ao2", "520 AO-MinVolts": "0.0000", "COM9-Parity": "None", "PRIME-CircularBufferEnabled": "ON", "PRIME-PP 6 Reset": "No", "640 AO-Description": "NI DAC", "Source": "PRIME", "445 AO-MinVolts": "0.0000", "PIEZO-Name": "Stage", "445 AO-Description": "NI DAC", "PRIME-CircularBufferFrameCount": "46", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "520 AO-Volts": "4.5000", "528 Shutter-TriggerSampleRate": "100000", "XPositionUm": 23269.100000000002, "OpticalPath-State": "4", "PRIME-PP 3 THRESHOLD": "80", "PRIME-PVCAM Version": "3.7.1", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Gain": "1-HDR", "640 Shutter-TriggerSequenceLength": "1000", "528 AO-SupportsTriggering": "0", "445 AO-TriggerInputLine": "/Dev1/PFI0", "OpticalPath-Name": "OpticalPath", "PRIME-PP 4 ENABLED": "Yes", "PRIME-PVCAM Adapter Version": "1.3.29", "COM3-Name": "COM3", "405 AO-MaxVolts": "5.0000", "520 Shutter-Block voltage": "false", "PRIME-Timing-ClearingTimeNs": "49344000.0000", "Core-Focus": "PIEZO", "528 AO-Block voltage": "false", "TE2000-Description": "Nikon TE2000 hub", "528 AO-MinVolts": "0.0000", "COM4-Parity": "None", "Summary": { "Slices": 11, "ChContrastMin": [ 0, 0, 0, 0, 0, 0 ], "PixelAspect": 1, "SlicesFirst": true, "Interval_ms": 0, "Time": "2019-12-17 03:59:33 -0500", "Prefix": "seq_7", "InitialPositionList": [ { "Label": "Pos0", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [149.91], "Focus": [16657.7], "XYStage": [ 71629.7, 19152.9 ] }, "GridColumnIndex": 0 }, { "Label": "Pos1", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [190.82], "Focus": [16485.4], "XYStage": [ 65702.3, 19396.600000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos2", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [194.69], "Focus": [16446.8], "XYStage": [ 59880.5, 19007.8 ] }, "GridColumnIndex": 0 }, { "Label": "Pos3", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [145.04], "Focus": [16446.6], "XYStage": [ 53616.9, 18818.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos4", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [169.57500000000002], "Focus": [16446.5], "XYStage": [ 47662.8, 18817.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos5", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [131.805], "Focus": [16446.4], "XYStage": [ 41918.8, 18298.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos6", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [113.145], "Focus": [16446.5], "XYStage": [ 36129.9, 18895.8 ] }, "GridColumnIndex": 0 }, { "Label": "Pos7", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [141.005], "Focus": [16446.6], "XYStage": [ 30299.100000000002, 18895.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos8", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [140.97], "Focus": [16446.5], "XYStage": [ 23952.100000000002, 19156 ] }, "GridColumnIndex": 0 }, { "Label": "Pos9", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [184.57500000000002], "Focus": [16677.9], "XYStage": [ 72866.8, -14809.300000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos10", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [167.95000000000002], "Focus": [16557.9], "XYStage": [ 66515.6, -14371.300000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos11", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [168], "Focus": [16618.9], "XYStage": [ 59724.3, -13964.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos12", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [203.205], "Focus": [16779.6], "XYStage": [ 53862.3, -13494.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos13", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [203.20000000000002], "Focus": [16749], "XYStage": [ 48108.3, -13805.400000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos14", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [203.19], "Focus": [16675.9], "XYStage": [ 41645, -13804.900000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos15", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [203.21], "Focus": [16675.7], "XYStage": [ 35352.6, -13805.6 ] }, "GridColumnIndex": 0 }, { "Label": "Pos16", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [203.225], "Focus": [16675.8], "XYStage": [ 29298, -13447.400000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos17", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [203.215], "Focus": [16675.8], "XYStage": [ 23269.100000000002, -13012.300000000001 ] }, "GridColumnIndex": 0 } ], "z-step_um": 10, "GridRow": 0, "BitDepth": 16, "Depth": 2, "Channels": 6, "Source": "Micro-Manager", "ChColors": [ -16711681, -256, -65281, -1, -1, -16776961 ], "MetadataVersion": 10, "KeepShutterOpenChannels": true, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536, 65536 ], "CustomIntervals_ms": [], "Height": 1200, "MicroManagerVersion": "1.4.23 20180219", "GridColumn": 0, "UUID": "3ad8801b-5699-40d6-813b-4ad840863e38", "PixelType": "GRAY16", "Width": 1200, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\Yan\\20191216-YC113-S11S12-miseq", "Frames": 1, "Comment": "", "UserName": "Nikon", "PixelSize_um": 1.1, "ROI": [ 0, 0, 1200, 1200 ], "Date": "2019-12-17", "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC", "P-DAPI-high" ], "KeepShutterOpenSlices": true, "IJType": 1, "ComputerName": "ZadorConfocal2", "Positions": 18, "PositionIndex": 17, "CameraTimeout": 20000, "TimeFirst": false }, "640 AO-Name": "AnalogIO", "Vincent-D3-Command": "Close" }, "FrameKey-0-0-10": { "COM4-StopBits": "1", "528 AO-MaxVolts": "5.0000", "XLIGHT Excitation Wheel-State": "1", "470 Shutter-Name": "AnalogIO", "Core-Shutter": "520", "555 AO-Demo": "No", "XLIGHT Dichroic-State": "2", "XYStage-StepSize-Y": "0.1000", "ZPositionUm": 253.215, "XYStage-StepSize-X": "0.1000", "PRIME-FirmwareVersion": "20.28", "PRIME-ClearCycles": "2", "520-Name": "DA Shutter", "405 Shutter-Name": "AnalogIO", "PIEZO-StepSize": "0.0050", "PRIME-ClearMode": "Pre-Exposure", "470 AO-MinVolts": "0.0000", "405 AO-SupportsTriggering": "1", "CameraChannelIndex": 0, "XYStage-StartSpeed": "500.0000", "COM9-StopBits": "2", "405 AO-Description": "NI DAC", "COM4-DelayBetweenCharsMs": "0.0000", "445 AO-Block voltage": "false", "520 AO-TriggerInputLine": "/Dev1/PFI0", "555 AO-Description": "NI DAC", "520 AO-TriggerSampleRate": "100000", "COM4-Verbose": "1", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM4-Name": "COM4", "405 Shutter-Volts": "0.0000", "Vincent-D3-Port": "COM1", "PRIME-PP 4 MIN ADU AFFECTED": "200", "470 Shutter-MinVolts": "0.0000", "PRIME-PP 3 ENABLED": "Yes", "470 AO-Description": "NI DAC", "PRIME-Description": "PVCAM API device adapter", "520 Shutter-MaxVolts": "5.0000", "520 Shutter-Volts": "5.0000", "528 AO-Sequenceable": "1", "PRIME-Binning": "1x1", "555 Shutter-MaxVolts": "5.0000", "528 Shutter-TriggerSequenceLength": "1000", "405 Shutter-Block voltage": "false", "XLIGHT Spin motor-State": "1", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "Vincent-D3-Time to open (ms)": "35.0000", "Vincent-D3-Time to close (ms)": "35.0000", "445 AO-TriggerSampleRate": "100000", "640 Shutter-IOChannel": "Dev1/ao14", "555 AO-Sequenceable": "1", "520 AO-Name": "AnalogIO", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "470 AO-Block voltage": "false", "COM1-Verbose": "1", "COM9-AnswerTimeout": "10000.0000", "528 Shutter-Name": "AnalogIO", "PRIME-Trigger Timeout (secs)": "10", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "NextFrame": 0, "470 AO-TriggerSequenceLength": "1000", "PRIME-TransposeXY": "0", "445 AO-TriggerSequenceLength": "1000", "COM1-Name": "COM1", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "COM9-DataBits": "8", "COM1-AnswerTimeout": "500.0000", "Camera": "", "TE2000-Version": "V2.50-V2.50-V2.10", "528 Shutter-Volts": "0.0000", "XLIGHT Hub-Name": "XLIGHT Hub", "PIEZO-Description": "Ludl stage driver adapter", "PositionName": "Pos17", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSampleRate": "100000", "PRIME-FanSpeedSetpoint": "High", "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "405 AO-TriggerSampleRate": "100000", "470 Shutter-Block voltage": "false", "470-State": "0", "COM3-Parity": "None", "405 AO-Volts": "0.0000", "PRIME-TransposeMirrorY": "0", "PRIME-ChipName": "GS144BSI", "470 AO-Volts": "0.0000", "PRIME-TransposeMirrorX": "0", "445 Shutter-SupportsTriggering": "0", "470 Shutter-SupportsTriggering": "0", "XLIGHT Hub-Description": "XLIGHT hub", "PRIME-PreampOffLimit": "10000", "470 AO-Sequenceable": "1", "528 Shutter-MaxVolts": "5.0000", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "Core-XYStage": "XYStage", "470 AO-TriggerSampleRate": "100000", "PRIME-Timing-PreTriggerDelayNs": "24672000.0000", "528-DA Device": "528 Shutter", "Focus-Description": "Nikon TE2000 focus adapter", "FilterBlock-State": "5", "520 AO-Demo": "No", "COM3-DelayBetweenCharsMs": "0.0000", "470 Shutter-Volts": "0.0000", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "PRIME-PP 2 THRESHOLD": "125", "PRIME-PP 4 THRESHOLD": "75", "XYStage-Acceleration": "4.0000", "470 Shutter-Demo": "No", "PRIME-Name": "PMPCIECam00", "640-DA Device": "640 Shutter", "405 AO-Block voltage": "false", "ElapsedTime-ms": 721956, "PRIME-Offset": "100", "445 AO-IOChannel": "Dev1/ao1", "640 AO-MaxVolts": "5.0000", "PositionIndex": 17, "PRIME-PP 1 ENABLED": "Yes", "COM1-BaudRate": "9600", "640-State": "0", "COM3-Verbose": "1", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "LudlController-TransmissionDelay": "0", "PRIME-FullWellCapacity": "62000", "PRIME-SMARTStreamingEnabled": "No", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "640 Shutter-Description": "NI DAC", "528-Description": "DA device that is used as a shutter", "COM4-Description": "Serial port driver (boost:asio)", "405 Shutter-Description": "NI DAC", "TE2000-Name": "TE2000", "528 Shutter-Description": "NI DAC", "COM9-Description": "Serial port driver (boost:asio)", "LudlController-Name": "LudlController", "445 Shutter-TriggerSequenceLength": "1000", "PRIME-SerialNumber": "A18B203008", "445 AO-Volts": "0.0000", "PRIME-X-dimension": "1200", "PRIME-ShutterCloseDelay": "0", "XLIGHT Disk slider-State": "2", "Position": "Default", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 AO-IOChannel": "Dev1/ao5", "520 AO-IOChannel": "Dev1/ao4", "528 AO-TriggerSequenceLength": "1000", "405 Shutter-MaxVolts": "5.0000", "470-DA Device": "470 Shutter", "PRIME-FTCapable": "No", "Core-ChannelGroup": "Channels", "555 AO-SupportsTriggering": "0", "PRIME-Y-dimension": "1200", "Frame": 0, "555 Shutter-Sequenceable": "1", "PRIME-Timing-ExposureTimeNs": "499998000.0000", "Core-ImageProcessor": "", "528-State": "0", "520 AO-Block voltage": "false", "XLIGHT Excitation Wheel-Label": "CFP/YFP", "640 Shutter-Demo": "No", "COM3-StopBits": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "520 Shutter-IOChannel": "Dev1/ao11", "Width": 1200, "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-SupportsTriggering": "0", "640 AO-TriggerSampleRate": "100000", "520-Description": "DA device that is used as a shutter", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "405 Shutter-Sequenceable": "1", "405 Shutter-SupportsTriggering": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-TriggerSampleRate": "100000", "640 Shutter-MaxVolts": "5.0000", "555 AO-MaxVolts": "5.0000", "520 AO-TriggerSequenceLength": "1000", "COM3-BaudRate": "9600", "COM9-BaudRate": "9600", "Vincent-D3-Channel #": "Ch. #1", "PRIME-PMode": "Normal", "OpticalPath-Label": "Left(100)", "BitDepth": 16, "555 AO-TriggerSequenceLength": "1000", "445 Shutter-Description": "NI DAC", "405 AO-Sequenceable": "1", "PRIME-AcquisitionMethod": "Callbacks", "555 AO-Name": "AnalogIO", "PRIME-ShutterMode": "Pre-Exposure", "COM3-Handshaking": "Off", "445 AO-SupportsTriggering": "0", "ROI": "0-0-1200-1200", "COM9-Verbose": "1", "PRIME-Color": "OFF", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "PRIME-ShutterOpenDelay": "0", "COM1-Parity": "None", "Slice": 10, "SlicePosition": 50, "COM9-DelayBetweenCharsMs": "0.0000", "555-Name": "DA Shutter", "PIEZO-LudlSingleAxisName": "B", "XLIGHT Emission Wheel-Label": "565/24(G)", "640 Shutter-Name": "AnalogIO", "640 AO-Sequenceable": "1", "520 Shutter-Demo": "No", "528 Shutter-Demo": "No", "470-Name": "DA Shutter", "520-DA Device": "520 Shutter", "XYStage-Description": "Ludl XY stage driver adapter", "445 Shutter-MinVolts": "0.0000", "528 AO-TriggerSampleRate": "100000", "405 AO-MinVolts": "0.0000", "405 Shutter-TriggerSequenceLength": "1000", "Height": 1200, "555 AO-Block voltage": "false", "640 Shutter-Volts": "0.0000", "PixelType": "GRAY16", "445 Shutter-Block voltage": "false", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "520 Shutter-TriggerSequenceLength": "1000", "COM9-Name": "COM9", "Core-SLM": "", "555 AO-Volts": "0.0000", "555 Shutter-Block voltage": "false", "XYStage-Speed": "1800.0000", "520 AO-MaxVolts": "5.0000", "528 Shutter-SupportsTriggering": "0", "COM1-Description": "Serial port driver (boost:asio)", "PRIME-MetadataEnabled": "No", "520 Shutter-MinVolts": "0.0000", "470 Shutter-Sequenceable": "1", "PRIME-PreampDelay": "0", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-MinVolts": "0.0000", "ChannelIndex": 0, "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Core-AutoShutter": "1", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "Exposure-ms": 320, "PRIME-PP 2 ENABLED": "Yes", "640 Shutter-TriggerSampleRate": "100000", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "470 Shutter-IOChannel": "Dev1/ao10", "405 AO-Demo": "No", "PixelSizeUm": 1.1, "470 AO-Demo": "No", "470 Shutter-MaxVolts": "5.0000", "Vincent-D3-Name": "Vincent-D3", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FileName": "img_000000000_Il-G_010.tif", "LudlController-Reset": "Operate", "PRIME-Exposure": "320.00", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "445-Name": "DA Shutter", "555 Shutter-Name": "AnalogIO", "520 Shutter-TriggerSampleRate": "100000", "445 Shutter-IOChannel": "Dev1/ao9", "PRIME-TransposeCorrection": "0", "XYStage-StepSize": "0.1000", "445-Description": "DA device that is used as a shutter", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 AO-IOChannel": "Dev1/ao7", "520 Shutter-Name": "AnalogIO", "COM3-DataBits": "8", "640 AO-Volts": "0.0000", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "555 Shutter-IOChannel": "Dev1/ao13", "WaitInterval": null, "520 Shutter-Sequenceable": "1", "YPositionUm": -13012.300000000001, "445-State": "0", "445-DA Device": "445 Shutter", "PRIME-CircularBufferAutoSize": "ON", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "555 AO-TriggerSampleRate": "100000", "445 Shutter-Volts": "0.0000", "XLIGHT Hub-Port": "COM3", "Core-AutoFocus": "", "COM4-Handshaking": "Off", "528 Shutter-MinVolts": "0.0000", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "445 Shutter-Name": "AnalogIO", "528 Shutter-Sequenceable": "1", "XYStage-TransposeMirrorX": "0", "XYStage-TransposeMirrorY": "0", "PRIME-ReadoutRate": "100MHz 16bit", "XYStage-Name": "Stage", "640 AO-SupportsTriggering": "0", "640-Name": "DA Shutter", "640 Shutter-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "640 AO-Block voltage": "false", "445 Shutter-Sequenceable": "1", "470 AO-MaxVolts": "5.0000", "COM4-BaudRate": "9600", "470-Description": "DA device that is used as a shutter", "520 Shutter-Description": "NI DAC", "PRIME-CCDTemperature": "-14.9200", "Time": "2019-12-17 03:59:38 -0500", "640 AO-TriggerSequenceLength": "1000", "470 AO-Name": "AnalogIO", "XLIGHT Disk slider-Label": "Disk Pos 40um", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "Binning": "1x1", "TE2000-ModelType": "1", "COM3-AnswerTimeout": "500.0000", "555 Shutter-SupportsTriggering": "0", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "TE2000-Port": "COM4", "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Description": "NI DAC", "COM4-AnswerTimeout": "500.0000", "COM1-DelayBetweenCharsMs": "0.0000", "FilterBlock-Label": "Confocal", "445 AO-Name": "AnalogIO", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-Timing-ReadoutTimeNs": "24672000.0000", "528 AO-Demo": "No", "520 AO-Sequenceable": "1", "555-Description": "DA device that is used as a shutter", "405-DA Device": "405 Shutter", "405 AO-TriggerSequenceLength": "1000", "640 AO-MinVolts": "0.0000", "640 Shutter-Block voltage": "false", "FrameIndex": 0, "PRIME-Port": "CMOS", "555 AO-MinVolts": "0.0000", "COM3-Description": "Serial port driver (boost:asio)", "528 AO-Description": "NI DAC", "555 AO-IOChannel": "Dev1/ao6", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "555 AO-TriggerInputLine": "/Dev1/PFI0", "PRIME-ActualInterval-ms": "84.6200", "LudlController-Port": "COM9", "445 Shutter-Demo": "No", "PRIME-Trigger-Expose Out-Mux": "1", "SliceIndex": 10, "PRIME-CCDTemperatureSetPoint": "-15.0000", "405 Shutter-MinVolts": "0.0000", "PRIME-ExposeOutMode": "First Row", "AxisPositions": null, "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Volts": "0.0000", "528-Name": "DA Shutter", "555-State": "0", "XLIGHT Dichroic-Label": "CFP/YFP", "445 AO-Sequenceable": "1", "405-Name": "DA Shutter", "405 AO-IOChannel": "Dev1/ao0", "Core-Camera": "PRIME", "Focus-Name": "Focus", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "405 Shutter-IOChannel": "Dev1/ao8", "640 AO-Demo": "No", "640 Shutter-SupportsTriggering": "0", "445 AO-Demo": "No", "555-DA Device": "555 Shutter", "470 Shutter-Description": "NI DAC", "528 AO-Volts": "0.0000", "COM1-DataBits": "8", "Vincent-D3-Address": "x", "520 AO-SupportsTriggering": "0", "405-Description": "DA device that is used as a shutter", "640 Shutter-MinVolts": "0.0000", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "405 AO-Name": "AnalogIO", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "640 AO-TriggerInputLine": "/Dev1/PFI0", "405 AO-TriggerInputLine": "/Dev1/PFI0", "640-Description": "DA device that is used as a shutter", "445 Shutter-TriggerSampleRate": "100000", "470 Shutter-TriggerSequenceLength": "1000", "COM1-StopBits": "1", "520 Shutter-SupportsTriggering": "0", "405-State": "0", "Core-Initialize": "1", "XLIGHT Emission Wheel-State": "3", "520 AO-Description": "NI DAC", "528 AO-TriggerInputLine": "/Dev1/PFI0", "445 Shutter-MaxVolts": "5.0000", "COM9-Handshaking": "Off", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "XLIGHT Spin motor-Label": "On (spinning)", "PRIME-PP 5 ENABLED": "No", "UUID": "9d6d2c0d-6936-4f81-99ca-2ee9ed2781c5", "PRIME-TriggerMode": "Internal Trigger", "COM1-Handshaking": "Off", "PRIME-Timing-PostTriggerDelayNs": "49344000.0000", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "Focus-Position": "16732.9000", "528 Shutter-Block voltage": "false", "Channel": "Il-G", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "555 Shutter-Demo": "No", "405 Shutter-Demo": "No", "528 AO-Name": "AnalogIO", "520-State": "0", "Core-Galvo": "", "405 Shutter-TriggerSampleRate": "100000", "Focus-FocusStepSize_(nm)": "100", "PRIME-PixelType": "16bit", "COM4-DataBits": "8", "470 AO-IOChannel": "Dev1/ao2", "520 AO-MinVolts": "0.0000", "COM9-Parity": "None", "PRIME-CircularBufferEnabled": "ON", "PRIME-PP 6 Reset": "No", "640 AO-Description": "NI DAC", "Source": "PRIME", "445 AO-MinVolts": "0.0000", "PIEZO-Name": "Stage", "445 AO-Description": "NI DAC", "PRIME-CircularBufferFrameCount": "46", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "520 AO-Volts": "4.5000", "528 Shutter-TriggerSampleRate": "100000", "XPositionUm": 23269.100000000002, "OpticalPath-State": "4", "PRIME-PP 3 THRESHOLD": "80", "PRIME-PVCAM Version": "3.7.1", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Gain": "1-HDR", "640 Shutter-TriggerSequenceLength": "1000", "528 AO-SupportsTriggering": "0", "445 AO-TriggerInputLine": "/Dev1/PFI0", "OpticalPath-Name": "OpticalPath", "PRIME-PP 4 ENABLED": "Yes", "PRIME-PVCAM Adapter Version": "1.3.29", "COM3-Name": "COM3", "405 AO-MaxVolts": "5.0000", "520 Shutter-Block voltage": "false", "PRIME-Timing-ClearingTimeNs": "49344000.0000", "Core-Focus": "PIEZO", "528 AO-Block voltage": "false", "TE2000-Description": "Nikon TE2000 hub", "528 AO-MinVolts": "0.0000", "COM4-Parity": "None", "Summary": { "Slices": 11, "ChContrastMin": [ 0, 0, 0, 0, 0, 0 ], "PixelAspect": 1, "SlicesFirst": true, "Interval_ms": 0, "Time": "2019-12-17 03:59:33 -0500", "Prefix": "seq_7", "InitialPositionList": [ { "Label": "Pos0", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [149.91], "Focus": [16657.7], "XYStage": [ 71629.7, 19152.9 ] }, "GridColumnIndex": 0 }, { "Label": "Pos1", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [190.82], "Focus": [16485.4], "XYStage": [ 65702.3, 19396.600000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos2", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [194.69], "Focus": [16446.8], "XYStage": [ 59880.5, 19007.8 ] }, "GridColumnIndex": 0 }, { "Label": "Pos3", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [145.04], "Focus": [16446.6], "XYStage": [ 53616.9, 18818.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos4", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [169.57500000000002], "Focus": [16446.5], "XYStage": [ 47662.8, 18817.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos5", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [131.805], "Focus": [16446.4], "XYStage": [ 41918.8, 18298.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos6", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [113.145], "Focus": [16446.5], "XYStage": [ 36129.9, 18895.8 ] }, "GridColumnIndex": 0 }, { "Label": "Pos7", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [141.005], "Focus": [16446.6], "XYStage": [ 30299.100000000002, 18895.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos8", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [140.97], "Focus": [16446.5], "XYStage": [ 23952.100000000002, 19156 ] }, "GridColumnIndex": 0 }, { "Label": "Pos9", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [184.57500000000002], "Focus": [16677.9], "XYStage": [ 72866.8, -14809.300000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos10", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [167.95000000000002], "Focus": [16557.9], "XYStage": [ 66515.6, -14371.300000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos11", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [168], "Focus": [16618.9], "XYStage": [ 59724.3, -13964.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos12", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [203.205], "Focus": [16779.6], "XYStage": [ 53862.3, -13494.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos13", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [203.20000000000002], "Focus": [16749], "XYStage": [ 48108.3, -13805.400000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos14", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [203.19], "Focus": [16675.9], "XYStage": [ 41645, -13804.900000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos15", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [203.21], "Focus": [16675.7], "XYStage": [ 35352.6, -13805.6 ] }, "GridColumnIndex": 0 }, { "Label": "Pos16", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [203.225], "Focus": [16675.8], "XYStage": [ 29298, -13447.400000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos17", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [203.215], "Focus": [16675.8], "XYStage": [ 23269.100000000002, -13012.300000000001 ] }, "GridColumnIndex": 0 } ], "z-step_um": 10, "GridRow": 0, "BitDepth": 16, "Depth": 2, "Channels": 6, "Source": "Micro-Manager", "ChColors": [ -16711681, -256, -65281, -1, -1, -16776961 ], "MetadataVersion": 10, "KeepShutterOpenChannels": true, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536, 65536 ], "CustomIntervals_ms": [], "Height": 1200, "MicroManagerVersion": "1.4.23 20180219", "GridColumn": 0, "UUID": "3ad8801b-5699-40d6-813b-4ad840863e38", "PixelType": "GRAY16", "Width": 1200, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\Yan\\20191216-YC113-S11S12-miseq", "Frames": 1, "Comment": "", "UserName": "Nikon", "PixelSize_um": 1.1, "ROI": [ 0, 0, 1200, 1200 ], "Date": "2019-12-17", "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC", "P-DAPI-high" ], "KeepShutterOpenSlices": true, "IJType": 1, "ComputerName": "ZadorConfocal2", "Positions": 18, "PositionIndex": 17, "CameraTimeout": 20000, "TimeFirst": false }, "640 AO-Name": "AnalogIO", "Vincent-D3-Command": "Close" }, "FrameKey-0-1-0": { "COM4-StopBits": "1", "528 AO-MaxVolts": "5.0000", "XLIGHT Excitation Wheel-State": "1", "470 Shutter-Name": "AnalogIO", "Core-Shutter": "555", "555 AO-Demo": "No", "XLIGHT Dichroic-State": "2", "XYStage-StepSize-Y": "0.1000", "ZPositionUm": 153.215, "XYStage-StepSize-X": "0.1000", "PRIME-FirmwareVersion": "20.28", "PRIME-ClearCycles": "2", "520-Name": "DA Shutter", "405 Shutter-Name": "AnalogIO", "PIEZO-StepSize": "0.0050", "PRIME-ClearMode": "Pre-Exposure", "470 AO-MinVolts": "0.0000", "405 AO-SupportsTriggering": "1", "CameraChannelIndex": 0, "XYStage-StartSpeed": "500.0000", "COM9-StopBits": "2", "405 AO-Description": "NI DAC", "COM4-DelayBetweenCharsMs": "0.0000", "445 AO-Block voltage": "false", "520 AO-TriggerInputLine": "/Dev1/PFI0", "555 AO-Description": "NI DAC", "520 AO-TriggerSampleRate": "100000", "COM4-Verbose": "1", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM4-Name": "COM4", "405 Shutter-Volts": "0.0000", "Vincent-D3-Port": "COM1", "PRIME-PP 4 MIN ADU AFFECTED": "200", "470 Shutter-MinVolts": "0.0000", "PRIME-PP 3 ENABLED": "Yes", "470 AO-Description": "NI DAC", "PRIME-Description": "PVCAM API device adapter", "520 Shutter-MaxVolts": "5.0000", "520 Shutter-Volts": "0.0000", "528 AO-Sequenceable": "1", "PRIME-Binning": "1x1", "555 Shutter-MaxVolts": "5.0000", "528 Shutter-TriggerSequenceLength": "1000", "405 Shutter-Block voltage": "false", "XLIGHT Spin motor-State": "1", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "Vincent-D3-Time to open (ms)": "35.0000", "Vincent-D3-Time to close (ms)": "35.0000", "445 AO-TriggerSampleRate": "100000", "640 Shutter-IOChannel": "Dev1/ao14", "555 AO-Sequenceable": "1", "520 AO-Name": "AnalogIO", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "470 AO-Block voltage": "false", "COM1-Verbose": "1", "COM9-AnswerTimeout": "10000.0000", "528 Shutter-Name": "AnalogIO", "PRIME-Trigger Timeout (secs)": "10", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "NextFrame": 0, "470 AO-TriggerSequenceLength": "1000", "PRIME-TransposeXY": "0", "445 AO-TriggerSequenceLength": "1000", "COM1-Name": "COM1", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "COM9-DataBits": "8", "COM1-AnswerTimeout": "500.0000", "Camera": "", "TE2000-Version": "V2.50-V2.50-V2.10", "528 Shutter-Volts": "0.0000", "XLIGHT Hub-Name": "XLIGHT Hub", "PIEZO-Description": "Ludl stage driver adapter", "PositionName": "Pos17", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSampleRate": "100000", "PRIME-FanSpeedSetpoint": "High", "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "405 AO-TriggerSampleRate": "100000", "470 Shutter-Block voltage": "false", "470-State": "0", "COM3-Parity": "None", "405 AO-Volts": "0.0000", "PRIME-TransposeMirrorY": "0", "PRIME-ChipName": "GS144BSI", "470 AO-Volts": "0.0000", "PRIME-TransposeMirrorX": "0", "445 Shutter-SupportsTriggering": "0", "470 Shutter-SupportsTriggering": "0", "XLIGHT Hub-Description": "XLIGHT hub", "PRIME-PreampOffLimit": "10000", "470 AO-Sequenceable": "1", "528 Shutter-MaxVolts": "5.0000", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "Core-XYStage": "XYStage", "470 AO-TriggerSampleRate": "100000", "PRIME-Timing-PreTriggerDelayNs": "24672000.0000", "528-DA Device": "528 Shutter", "Focus-Description": "Nikon TE2000 focus adapter", "FilterBlock-State": "5", "520 AO-Demo": "No", "COM3-DelayBetweenCharsMs": "0.0000", "470 Shutter-Volts": "0.0000", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "PRIME-PP 2 THRESHOLD": "125", "PRIME-PP 4 THRESHOLD": "75", "XYStage-Acceleration": "4.0000", "470 Shutter-Demo": "No", "PRIME-Name": "PMPCIECam00", "640-DA Device": "640 Shutter", "405 AO-Block voltage": "false", "ElapsedTime-ms": 723236, "PRIME-Offset": "100", "445 AO-IOChannel": "Dev1/ao1", "640 AO-MaxVolts": "5.0000", "PositionIndex": 17, "PRIME-PP 1 ENABLED": "Yes", "COM1-BaudRate": "9600", "640-State": "0", "COM3-Verbose": "1", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "LudlController-TransmissionDelay": "0", "PRIME-FullWellCapacity": "62000", "PRIME-SMARTStreamingEnabled": "No", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "640 Shutter-Description": "NI DAC", "528-Description": "DA device that is used as a shutter", "COM4-Description": "Serial port driver (boost:asio)", "405 Shutter-Description": "NI DAC", "TE2000-Name": "TE2000", "528 Shutter-Description": "NI DAC", "COM9-Description": "Serial port driver (boost:asio)", "LudlController-Name": "LudlController", "445 Shutter-TriggerSequenceLength": "1000", "PRIME-SerialNumber": "A18B203008", "445 AO-Volts": "0.0000", "PRIME-X-dimension": "1200", "PRIME-ShutterCloseDelay": "0", "XLIGHT Disk slider-State": "2", "Position": "Default", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 AO-IOChannel": "Dev1/ao5", "520 AO-IOChannel": "Dev1/ao4", "528 AO-TriggerSequenceLength": "1000", "405 Shutter-MaxVolts": "5.0000", "470-DA Device": "470 Shutter", "PRIME-FTCapable": "No", "Core-ChannelGroup": "Channels", "555 AO-SupportsTriggering": "0", "PRIME-Y-dimension": "1200", "Frame": 0, "555 Shutter-Sequenceable": "1", "PRIME-Timing-ExposureTimeNs": "499998000.0000", "Core-ImageProcessor": "", "528-State": "0", "520 AO-Block voltage": "false", "XLIGHT Excitation Wheel-Label": "QUAD", "640 Shutter-Demo": "No", "COM3-StopBits": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "520 Shutter-IOChannel": "Dev1/ao11", "Width": 1200, "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-SupportsTriggering": "0", "640 AO-TriggerSampleRate": "100000", "520-Description": "DA device that is used as a shutter", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "405 Shutter-Sequenceable": "1", "405 Shutter-SupportsTriggering": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-TriggerSampleRate": "100000", "640 Shutter-MaxVolts": "5.0000", "555 AO-MaxVolts": "5.0000", "520 AO-TriggerSequenceLength": "1000", "COM3-BaudRate": "9600", "COM9-BaudRate": "9600", "Vincent-D3-Channel #": "Ch. #1", "PRIME-PMode": "Normal", "OpticalPath-Label": "Left(100)", "BitDepth": 16, "555 AO-TriggerSequenceLength": "1000", "445 Shutter-Description": "NI DAC", "405 AO-Sequenceable": "1", "PRIME-AcquisitionMethod": "Callbacks", "555 AO-Name": "AnalogIO", "PRIME-ShutterMode": "Pre-Exposure", "COM3-Handshaking": "Off", "445 AO-SupportsTriggering": "0", "ROI": "0-0-1200-1200", "COM9-Verbose": "1", "PRIME-Color": "OFF", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "PRIME-ShutterOpenDelay": "0", "COM1-Parity": "None", "Slice": 0, "SlicePosition": -50, "COM9-DelayBetweenCharsMs": "0.0000", "555-Name": "DA Shutter", "PIEZO-LudlSingleAxisName": "B", "XLIGHT Emission Wheel-Label": "585/11(T)", "640 Shutter-Name": "AnalogIO", "640 AO-Sequenceable": "1", "520 Shutter-Demo": "No", "528 Shutter-Demo": "No", "470-Name": "DA Shutter", "520-DA Device": "520 Shutter", "XYStage-Description": "Ludl XY stage driver adapter", "445 Shutter-MinVolts": "0.0000", "528 AO-TriggerSampleRate": "100000", "405 AO-MinVolts": "0.0000", "405 Shutter-TriggerSequenceLength": "1000", "Height": 1200, "555 AO-Block voltage": "false", "640 Shutter-Volts": "0.0000", "PixelType": "GRAY16", "445 Shutter-Block voltage": "false", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "520 Shutter-TriggerSequenceLength": "1000", "COM9-Name": "COM9", "Core-SLM": "", "555 AO-Volts": "4.5000", "555 Shutter-Block voltage": "false", "XYStage-Speed": "1800.0000", "520 AO-MaxVolts": "5.0000", "528 Shutter-SupportsTriggering": "0", "COM1-Description": "Serial port driver (boost:asio)", "PRIME-MetadataEnabled": "No", "520 Shutter-MinVolts": "0.0000", "470 Shutter-Sequenceable": "1", "PRIME-PreampDelay": "0", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-MinVolts": "0.0000", "ChannelIndex": 1, "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Core-AutoShutter": "1", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "Exposure-ms": 160, "PRIME-PP 2 ENABLED": "Yes", "640 Shutter-TriggerSampleRate": "100000", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "470 Shutter-IOChannel": "Dev1/ao10", "405 AO-Demo": "No", "PixelSizeUm": 1.1, "470 AO-Demo": "No", "470 Shutter-MaxVolts": "5.0000", "Vincent-D3-Name": "Vincent-D3", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FileName": "img_000000000_Il-T_000.tif", "LudlController-Reset": "Operate", "PRIME-Exposure": "160.00", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "445-Name": "DA Shutter", "555 Shutter-Name": "AnalogIO", "520 Shutter-TriggerSampleRate": "100000", "445 Shutter-IOChannel": "Dev1/ao9", "PRIME-TransposeCorrection": "0", "XYStage-StepSize": "0.1000", "445-Description": "DA device that is used as a shutter", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 AO-IOChannel": "Dev1/ao7", "520 Shutter-Name": "AnalogIO", "COM3-DataBits": "8", "640 AO-Volts": "0.0000", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "555 Shutter-IOChannel": "Dev1/ao13", "WaitInterval": null, "520 Shutter-Sequenceable": "1", "YPositionUm": -13012.300000000001, "445-State": "0", "445-DA Device": "445 Shutter", "PRIME-CircularBufferAutoSize": "ON", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "555 AO-TriggerSampleRate": "100000", "445 Shutter-Volts": "0.0000", "XLIGHT Hub-Port": "COM3", "Core-AutoFocus": "", "COM4-Handshaking": "Off", "528 Shutter-MinVolts": "0.0000", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "445 Shutter-Name": "AnalogIO", "528 Shutter-Sequenceable": "1", "XYStage-TransposeMirrorX": "0", "XYStage-TransposeMirrorY": "0", "PRIME-ReadoutRate": "100MHz 16bit", "XYStage-Name": "Stage", "640 AO-SupportsTriggering": "0", "640-Name": "DA Shutter", "640 Shutter-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "640 AO-Block voltage": "false", "445 Shutter-Sequenceable": "1", "470 AO-MaxVolts": "5.0000", "COM4-BaudRate": "9600", "470-Description": "DA device that is used as a shutter", "520 Shutter-Description": "NI DAC", "PRIME-CCDTemperature": "-14.9200", "Time": "2019-12-17 03:59:40 -0500", "640 AO-TriggerSequenceLength": "1000", "470 AO-Name": "AnalogIO", "XLIGHT Disk slider-Label": "Disk Pos 40um", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "Binning": "1x1", "TE2000-ModelType": "1", "COM3-AnswerTimeout": "500.0000", "555 Shutter-SupportsTriggering": "0", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "TE2000-Port": "COM4", "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Description": "NI DAC", "COM4-AnswerTimeout": "500.0000", "COM1-DelayBetweenCharsMs": "0.0000", "FilterBlock-Label": "Confocal", "445 AO-Name": "AnalogIO", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-Timing-ReadoutTimeNs": "24672000.0000", "528 AO-Demo": "No", "520 AO-Sequenceable": "1", "555-Description": "DA device that is used as a shutter", "405-DA Device": "405 Shutter", "405 AO-TriggerSequenceLength": "1000", "640 AO-MinVolts": "0.0000", "640 Shutter-Block voltage": "false", "FrameIndex": 0, "PRIME-Port": "CMOS", "555 AO-MinVolts": "0.0000", "COM3-Description": "Serial port driver (boost:asio)", "528 AO-Description": "NI DAC", "555 AO-IOChannel": "Dev1/ao6", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "555 AO-TriggerInputLine": "/Dev1/PFI0", "PRIME-ActualInterval-ms": "84.6200", "LudlController-Port": "COM9", "445 Shutter-Demo": "No", "PRIME-Trigger-Expose Out-Mux": "1", "SliceIndex": 0, "PRIME-CCDTemperatureSetPoint": "-15.0000", "405 Shutter-MinVolts": "0.0000", "PRIME-ExposeOutMode": "First Row", "AxisPositions": null, "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Volts": "5.0000", "528-Name": "DA Shutter", "555-State": "1", "XLIGHT Dichroic-Label": "QUAD", "445 AO-Sequenceable": "1", "405-Name": "DA Shutter", "405 AO-IOChannel": "Dev1/ao0", "Core-Camera": "PRIME", "Focus-Name": "Focus", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "405 Shutter-IOChannel": "Dev1/ao8", "640 AO-Demo": "No", "640 Shutter-SupportsTriggering": "0", "445 AO-Demo": "No", "555-DA Device": "555 Shutter", "470 Shutter-Description": "NI DAC", "528 AO-Volts": "0.0000", "COM1-DataBits": "8", "Vincent-D3-Address": "x", "520 AO-SupportsTriggering": "0", "405-Description": "DA device that is used as a shutter", "640 Shutter-MinVolts": "0.0000", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "405 AO-Name": "AnalogIO", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "640 AO-TriggerInputLine": "/Dev1/PFI0", "405 AO-TriggerInputLine": "/Dev1/PFI0", "640-Description": "DA device that is used as a shutter", "445 Shutter-TriggerSampleRate": "100000", "470 Shutter-TriggerSequenceLength": "1000", "COM1-StopBits": "1", "520 Shutter-SupportsTriggering": "0", "405-State": "0", "Core-Initialize": "1", "XLIGHT Emission Wheel-State": "3", "520 AO-Description": "NI DAC", "528 AO-TriggerInputLine": "/Dev1/PFI0", "445 Shutter-MaxVolts": "5.0000", "COM9-Handshaking": "Off", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "XLIGHT Spin motor-Label": "On (spinning)", "PRIME-PP 5 ENABLED": "No", "UUID": "5492fdf0-b221-478a-bd9e-c5ae8e375a68", "PRIME-TriggerMode": "Internal Trigger", "COM1-Handshaking": "Off", "PRIME-Timing-PostTriggerDelayNs": "49344000.0000", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "Focus-Position": "16732.9000", "528 Shutter-Block voltage": "false", "Channel": "Il-T", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "555 Shutter-Demo": "No", "405 Shutter-Demo": "No", "528 AO-Name": "AnalogIO", "520-State": "0", "Core-Galvo": "", "405 Shutter-TriggerSampleRate": "100000", "Focus-FocusStepSize_(nm)": "100", "PRIME-PixelType": "16bit", "COM4-DataBits": "8", "470 AO-IOChannel": "Dev1/ao2", "520 AO-MinVolts": "0.0000", "COM9-Parity": "None", "PRIME-CircularBufferEnabled": "ON", "PRIME-PP 6 Reset": "No", "640 AO-Description": "NI DAC", "Source": "PRIME", "445 AO-MinVolts": "0.0000", "PIEZO-Name": "Stage", "445 AO-Description": "NI DAC", "PRIME-CircularBufferFrameCount": "46", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "520 AO-Volts": "0.0000", "528 Shutter-TriggerSampleRate": "100000", "XPositionUm": 23269.100000000002, "OpticalPath-State": "4", "PRIME-PP 3 THRESHOLD": "80", "PRIME-PVCAM Version": "3.7.1", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Gain": "1-HDR", "640 Shutter-TriggerSequenceLength": "1000", "528 AO-SupportsTriggering": "0", "445 AO-TriggerInputLine": "/Dev1/PFI0", "OpticalPath-Name": "OpticalPath", "PRIME-PP 4 ENABLED": "Yes", "PRIME-PVCAM Adapter Version": "1.3.29", "COM3-Name": "COM3", "405 AO-MaxVolts": "5.0000", "520 Shutter-Block voltage": "false", "PRIME-Timing-ClearingTimeNs": "49344000.0000", "Core-Focus": "PIEZO", "528 AO-Block voltage": "false", "TE2000-Description": "Nikon TE2000 hub", "528 AO-MinVolts": "0.0000", "COM4-Parity": "None", "Summary": { "Slices": 11, "ChContrastMin": [ 0, 0, 0, 0, 0, 0 ], "PixelAspect": 1, "SlicesFirst": true, "Interval_ms": 0, "Time": "2019-12-17 03:59:33 -0500", "Prefix": "seq_7", "InitialPositionList": [ { "Label": "Pos0", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [149.91], "Focus": [16657.7], "XYStage": [ 71629.7, 19152.9 ] }, "GridColumnIndex": 0 }, { "Label": "Pos1", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [190.82], "Focus": [16485.4], "XYStage": [ 65702.3, 19396.600000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos2", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [194.69], "Focus": [16446.8], "XYStage": [ 59880.5, 19007.8 ] }, "GridColumnIndex": 0 }, { "Label": "Pos3", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [145.04], "Focus": [16446.6], "XYStage": [ 53616.9, 18818.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos4", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [169.57500000000002], "Focus": [16446.5], "XYStage": [ 47662.8, 18817.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos5", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [131.805], "Focus": [16446.4], "XYStage": [ 41918.8, 18298.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos6", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [113.145], "Focus": [16446.5], "XYStage": [ 36129.9, 18895.8 ] }, "GridColumnIndex": 0 }, { "Label": "Pos7", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [141.005], "Focus": [16446.6], "XYStage": [ 30299.100000000002, 18895.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos8", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [140.97], "Focus": [16446.5], "XYStage": [ 23952.100000000002, 19156 ] }, "GridColumnIndex": 0 }, { "Label": "Pos9", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [184.57500000000002], "Focus": [16677.9], "XYStage": [ 72866.8, -14809.300000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos10", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [167.95000000000002], "Focus": [16557.9], "XYStage": [ 66515.6, -14371.300000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos11", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [168], "Focus": [16618.9], "XYStage": [ 59724.3, -13964.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos12", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [203.205], "Focus": [16779.6], "XYStage": [ 53862.3, -13494.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos13", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [203.20000000000002], "Focus": [16749], "XYStage": [ 48108.3, -13805.400000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos14", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [203.19], "Focus": [16675.9], "XYStage": [ 41645, -13804.900000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos15", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [203.21], "Focus": [16675.7], "XYStage": [ 35352.6, -13805.6 ] }, "GridColumnIndex": 0 }, { "Label": "Pos16", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [203.225], "Focus": [16675.8], "XYStage": [ 29298, -13447.400000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos17", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [203.215], "Focus": [16675.8], "XYStage": [ 23269.100000000002, -13012.300000000001 ] }, "GridColumnIndex": 0 } ], "z-step_um": 10, "GridRow": 0, "BitDepth": 16, "Depth": 2, "Channels": 6, "Source": "Micro-Manager", "ChColors": [ -16711681, -256, -65281, -1, -1, -16776961 ], "MetadataVersion": 10, "KeepShutterOpenChannels": true, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536, 65536 ], "CustomIntervals_ms": [], "Height": 1200, "MicroManagerVersion": "1.4.23 20180219", "GridColumn": 0, "UUID": "3ad8801b-5699-40d6-813b-4ad840863e38", "PixelType": "GRAY16", "Width": 1200, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\Yan\\20191216-YC113-S11S12-miseq", "Frames": 1, "Comment": "", "UserName": "Nikon", "PixelSize_um": 1.1, "ROI": [ 0, 0, 1200, 1200 ], "Date": "2019-12-17", "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC", "P-DAPI-high" ], "KeepShutterOpenSlices": true, "IJType": 1, "ComputerName": "ZadorConfocal2", "Positions": 18, "PositionIndex": 17, "CameraTimeout": 20000, "TimeFirst": false }, "640 AO-Name": "AnalogIO", "Vincent-D3-Command": "Close" }, "FrameKey-0-1-1": { "COM4-StopBits": "1", "528 AO-MaxVolts": "5.0000", "XLIGHT Excitation Wheel-State": "1", "470 Shutter-Name": "AnalogIO", "Core-Shutter": "555", "555 AO-Demo": "No", "XLIGHT Dichroic-State": "2", "XYStage-StepSize-Y": "0.1000", "ZPositionUm": 163.215, "XYStage-StepSize-X": "0.1000", "PRIME-FirmwareVersion": "20.28", "PRIME-ClearCycles": "2", "520-Name": "DA Shutter", "405 Shutter-Name": "AnalogIO", "PIEZO-StepSize": "0.0050", "PRIME-ClearMode": "Pre-Exposure", "470 AO-MinVolts": "0.0000", "405 AO-SupportsTriggering": "1", "CameraChannelIndex": 0, "XYStage-StartSpeed": "500.0000", "COM9-StopBits": "2", "405 AO-Description": "NI DAC", "COM4-DelayBetweenCharsMs": "0.0000", "445 AO-Block voltage": "false", "520 AO-TriggerInputLine": "/Dev1/PFI0", "555 AO-Description": "NI DAC", "520 AO-TriggerSampleRate": "100000", "COM4-Verbose": "1", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM4-Name": "COM4", "405 Shutter-Volts": "0.0000", "Vincent-D3-Port": "COM1", "PRIME-PP 4 MIN ADU AFFECTED": "200", "470 Shutter-MinVolts": "0.0000", "PRIME-PP 3 ENABLED": "Yes", "470 AO-Description": "NI DAC", "PRIME-Description": "PVCAM API device adapter", "520 Shutter-MaxVolts": "5.0000", "520 Shutter-Volts": "0.0000", "528 AO-Sequenceable": "1", "PRIME-Binning": "1x1", "555 Shutter-MaxVolts": "5.0000", "528 Shutter-TriggerSequenceLength": "1000", "405 Shutter-Block voltage": "false", "XLIGHT Spin motor-State": "1", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "Vincent-D3-Time to open (ms)": "35.0000", "Vincent-D3-Time to close (ms)": "35.0000", "445 AO-TriggerSampleRate": "100000", "640 Shutter-IOChannel": "Dev1/ao14", "555 AO-Sequenceable": "1", "520 AO-Name": "AnalogIO", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "470 AO-Block voltage": "false", "COM1-Verbose": "1", "COM9-AnswerTimeout": "10000.0000", "528 Shutter-Name": "AnalogIO", "PRIME-Trigger Timeout (secs)": "10", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "NextFrame": 0, "470 AO-TriggerSequenceLength": "1000", "PRIME-TransposeXY": "0", "445 AO-TriggerSequenceLength": "1000", "COM1-Name": "COM1", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "COM9-DataBits": "8", "COM1-AnswerTimeout": "500.0000", "Camera": "", "TE2000-Version": "V2.50-V2.50-V2.10", "528 Shutter-Volts": "0.0000", "XLIGHT Hub-Name": "XLIGHT Hub", "PIEZO-Description": "Ludl stage driver adapter", "PositionName": "Pos17", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSampleRate": "100000", "PRIME-FanSpeedSetpoint": "High", "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "405 AO-TriggerSampleRate": "100000", "470 Shutter-Block voltage": "false", "470-State": "0", "COM3-Parity": "None", "405 AO-Volts": "0.0000", "PRIME-TransposeMirrorY": "0", "PRIME-ChipName": "GS144BSI", "470 AO-Volts": "0.0000", "PRIME-TransposeMirrorX": "0", "445 Shutter-SupportsTriggering": "0", "470 Shutter-SupportsTriggering": "0", "XLIGHT Hub-Description": "XLIGHT hub", "PRIME-PreampOffLimit": "10000", "470 AO-Sequenceable": "1", "528 Shutter-MaxVolts": "5.0000", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "Core-XYStage": "XYStage", "470 AO-TriggerSampleRate": "100000", "PRIME-Timing-PreTriggerDelayNs": "24672000.0000", "528-DA Device": "528 Shutter", "Focus-Description": "Nikon TE2000 focus adapter", "FilterBlock-State": "5", "520 AO-Demo": "No", "COM3-DelayBetweenCharsMs": "0.0000", "470 Shutter-Volts": "0.0000", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "PRIME-PP 2 THRESHOLD": "125", "PRIME-PP 4 THRESHOLD": "75", "XYStage-Acceleration": "4.0000", "470 Shutter-Demo": "No", "PRIME-Name": "PMPCIECam00", "640-DA Device": "640 Shutter", "405 AO-Block voltage": "false", "ElapsedTime-ms": 723679, "PRIME-Offset": "100", "445 AO-IOChannel": "Dev1/ao1", "640 AO-MaxVolts": "5.0000", "PositionIndex": 17, "PRIME-PP 1 ENABLED": "Yes", "COM1-BaudRate": "9600", "640-State": "0", "COM3-Verbose": "1", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "LudlController-TransmissionDelay": "0", "PRIME-FullWellCapacity": "62000", "PRIME-SMARTStreamingEnabled": "No", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "640 Shutter-Description": "NI DAC", "528-Description": "DA device that is used as a shutter", "COM4-Description": "Serial port driver (boost:asio)", "405 Shutter-Description": "NI DAC", "TE2000-Name": "TE2000", "528 Shutter-Description": "NI DAC", "COM9-Description": "Serial port driver (boost:asio)", "LudlController-Name": "LudlController", "445 Shutter-TriggerSequenceLength": "1000", "PRIME-SerialNumber": "A18B203008", "445 AO-Volts": "0.0000", "PRIME-X-dimension": "1200", "PRIME-ShutterCloseDelay": "0", "XLIGHT Disk slider-State": "2", "Position": "Default", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 AO-IOChannel": "Dev1/ao5", "520 AO-IOChannel": "Dev1/ao4", "528 AO-TriggerSequenceLength": "1000", "405 Shutter-MaxVolts": "5.0000", "470-DA Device": "470 Shutter", "PRIME-FTCapable": "No", "Core-ChannelGroup": "Channels", "555 AO-SupportsTriggering": "0", "PRIME-Y-dimension": "1200", "Frame": 0, "555 Shutter-Sequenceable": "1", "PRIME-Timing-ExposureTimeNs": "499998000.0000", "Core-ImageProcessor": "", "528-State": "0", "520 AO-Block voltage": "false", "XLIGHT Excitation Wheel-Label": "QUAD", "640 Shutter-Demo": "No", "COM3-StopBits": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "520 Shutter-IOChannel": "Dev1/ao11", "Width": 1200, "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-SupportsTriggering": "0", "640 AO-TriggerSampleRate": "100000", "520-Description": "DA device that is used as a shutter", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "405 Shutter-Sequenceable": "1", "405 Shutter-SupportsTriggering": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-TriggerSampleRate": "100000", "640 Shutter-MaxVolts": "5.0000", "555 AO-MaxVolts": "5.0000", "520 AO-TriggerSequenceLength": "1000", "COM3-BaudRate": "9600", "COM9-BaudRate": "9600", "Vincent-D3-Channel #": "Ch. #1", "PRIME-PMode": "Normal", "OpticalPath-Label": "Left(100)", "BitDepth": 16, "555 AO-TriggerSequenceLength": "1000", "445 Shutter-Description": "NI DAC", "405 AO-Sequenceable": "1", "PRIME-AcquisitionMethod": "Callbacks", "555 AO-Name": "AnalogIO", "PRIME-ShutterMode": "Pre-Exposure", "COM3-Handshaking": "Off", "445 AO-SupportsTriggering": "0", "ROI": "0-0-1200-1200", "COM9-Verbose": "1", "PRIME-Color": "OFF", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "PRIME-ShutterOpenDelay": "0", "COM1-Parity": "None", "Slice": 1, "SlicePosition": -40, "COM9-DelayBetweenCharsMs": "0.0000", "555-Name": "DA Shutter", "PIEZO-LudlSingleAxisName": "B", "XLIGHT Emission Wheel-Label": "585/11(T)", "640 Shutter-Name": "AnalogIO", "640 AO-Sequenceable": "1", "520 Shutter-Demo": "No", "528 Shutter-Demo": "No", "470-Name": "DA Shutter", "520-DA Device": "520 Shutter", "XYStage-Description": "Ludl XY stage driver adapter", "445 Shutter-MinVolts": "0.0000", "528 AO-TriggerSampleRate": "100000", "405 AO-MinVolts": "0.0000", "405 Shutter-TriggerSequenceLength": "1000", "Height": 1200, "555 AO-Block voltage": "false", "640 Shutter-Volts": "0.0000", "PixelType": "GRAY16", "445 Shutter-Block voltage": "false", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "520 Shutter-TriggerSequenceLength": "1000", "COM9-Name": "COM9", "Core-SLM": "", "555 AO-Volts": "4.5000", "555 Shutter-Block voltage": "false", "XYStage-Speed": "1800.0000", "520 AO-MaxVolts": "5.0000", "528 Shutter-SupportsTriggering": "0", "COM1-Description": "Serial port driver (boost:asio)", "PRIME-MetadataEnabled": "No", "520 Shutter-MinVolts": "0.0000", "470 Shutter-Sequenceable": "1", "PRIME-PreampDelay": "0", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-MinVolts": "0.0000", "ChannelIndex": 1, "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Core-AutoShutter": "1", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "Exposure-ms": 160, "PRIME-PP 2 ENABLED": "Yes", "640 Shutter-TriggerSampleRate": "100000", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "470 Shutter-IOChannel": "Dev1/ao10", "405 AO-Demo": "No", "PixelSizeUm": 1.1, "470 AO-Demo": "No", "470 Shutter-MaxVolts": "5.0000", "Vincent-D3-Name": "Vincent-D3", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FileName": "img_000000000_Il-T_001.tif", "LudlController-Reset": "Operate", "PRIME-Exposure": "160.00", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "445-Name": "DA Shutter", "555 Shutter-Name": "AnalogIO", "520 Shutter-TriggerSampleRate": "100000", "445 Shutter-IOChannel": "Dev1/ao9", "PRIME-TransposeCorrection": "0", "XYStage-StepSize": "0.1000", "445-Description": "DA device that is used as a shutter", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 AO-IOChannel": "Dev1/ao7", "520 Shutter-Name": "AnalogIO", "COM3-DataBits": "8", "640 AO-Volts": "0.0000", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "555 Shutter-IOChannel": "Dev1/ao13", "WaitInterval": null, "520 Shutter-Sequenceable": "1", "YPositionUm": -13012.300000000001, "445-State": "0", "445-DA Device": "445 Shutter", "PRIME-CircularBufferAutoSize": "ON", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "555 AO-TriggerSampleRate": "100000", "445 Shutter-Volts": "0.0000", "XLIGHT Hub-Port": "COM3", "Core-AutoFocus": "", "COM4-Handshaking": "Off", "528 Shutter-MinVolts": "0.0000", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "445 Shutter-Name": "AnalogIO", "528 Shutter-Sequenceable": "1", "XYStage-TransposeMirrorX": "0", "XYStage-TransposeMirrorY": "0", "PRIME-ReadoutRate": "100MHz 16bit", "XYStage-Name": "Stage", "640 AO-SupportsTriggering": "0", "640-Name": "DA Shutter", "640 Shutter-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "640 AO-Block voltage": "false", "445 Shutter-Sequenceable": "1", "470 AO-MaxVolts": "5.0000", "COM4-BaudRate": "9600", "470-Description": "DA device that is used as a shutter", "520 Shutter-Description": "NI DAC", "PRIME-CCDTemperature": "-14.9200", "Time": "2019-12-17 03:59:40 -0500", "640 AO-TriggerSequenceLength": "1000", "470 AO-Name": "AnalogIO", "XLIGHT Disk slider-Label": "Disk Pos 40um", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "Binning": "1x1", "TE2000-ModelType": "1", "COM3-AnswerTimeout": "500.0000", "555 Shutter-SupportsTriggering": "0", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "TE2000-Port": "COM4", "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Description": "NI DAC", "COM4-AnswerTimeout": "500.0000", "COM1-DelayBetweenCharsMs": "0.0000", "FilterBlock-Label": "Confocal", "445 AO-Name": "AnalogIO", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-Timing-ReadoutTimeNs": "24672000.0000", "528 AO-Demo": "No", "520 AO-Sequenceable": "1", "555-Description": "DA device that is used as a shutter", "405-DA Device": "405 Shutter", "405 AO-TriggerSequenceLength": "1000", "640 AO-MinVolts": "0.0000", "640 Shutter-Block voltage": "false", "FrameIndex": 0, "PRIME-Port": "CMOS", "555 AO-MinVolts": "0.0000", "COM3-Description": "Serial port driver (boost:asio)", "528 AO-Description": "NI DAC", "555 AO-IOChannel": "Dev1/ao6", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "555 AO-TriggerInputLine": "/Dev1/PFI0", "PRIME-ActualInterval-ms": "84.6200", "LudlController-Port": "COM9", "445 Shutter-Demo": "No", "PRIME-Trigger-Expose Out-Mux": "1", "SliceIndex": 1, "PRIME-CCDTemperatureSetPoint": "-15.0000", "405 Shutter-MinVolts": "0.0000", "PRIME-ExposeOutMode": "First Row", "AxisPositions": null, "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Volts": "5.0000", "528-Name": "DA Shutter", "555-State": "1", "XLIGHT Dichroic-Label": "QUAD", "445 AO-Sequenceable": "1", "405-Name": "DA Shutter", "405 AO-IOChannel": "Dev1/ao0", "Core-Camera": "PRIME", "Focus-Name": "Focus", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "405 Shutter-IOChannel": "Dev1/ao8", "640 AO-Demo": "No", "640 Shutter-SupportsTriggering": "0", "445 AO-Demo": "No", "555-DA Device": "555 Shutter", "470 Shutter-Description": "NI DAC", "528 AO-Volts": "0.0000", "COM1-DataBits": "8", "Vincent-D3-Address": "x", "520 AO-SupportsTriggering": "0", "405-Description": "DA device that is used as a shutter", "640 Shutter-MinVolts": "0.0000", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "405 AO-Name": "AnalogIO", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "640 AO-TriggerInputLine": "/Dev1/PFI0", "405 AO-TriggerInputLine": "/Dev1/PFI0", "640-Description": "DA device that is used as a shutter", "445 Shutter-TriggerSampleRate": "100000", "470 Shutter-TriggerSequenceLength": "1000", "COM1-StopBits": "1", "520 Shutter-SupportsTriggering": "0", "405-State": "0", "Core-Initialize": "1", "XLIGHT Emission Wheel-State": "3", "520 AO-Description": "NI DAC", "528 AO-TriggerInputLine": "/Dev1/PFI0", "445 Shutter-MaxVolts": "5.0000", "COM9-Handshaking": "Off", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "XLIGHT Spin motor-Label": "On (spinning)", "PRIME-PP 5 ENABLED": "No", "UUID": "94b69dcc-c88d-444c-bcb8-db51223fd73f", "PRIME-TriggerMode": "Internal Trigger", "COM1-Handshaking": "Off", "PRIME-Timing-PostTriggerDelayNs": "49344000.0000", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "Focus-Position": "16732.9000", "528 Shutter-Block voltage": "false", "Channel": "Il-T", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "555 Shutter-Demo": "No", "405 Shutter-Demo": "No", "528 AO-Name": "AnalogIO", "520-State": "0", "Core-Galvo": "", "405 Shutter-TriggerSampleRate": "100000", "Focus-FocusStepSize_(nm)": "100", "PRIME-PixelType": "16bit", "COM4-DataBits": "8", "470 AO-IOChannel": "Dev1/ao2", "520 AO-MinVolts": "0.0000", "COM9-Parity": "None", "PRIME-CircularBufferEnabled": "ON", "PRIME-PP 6 Reset": "No", "640 AO-Description": "NI DAC", "Source": "PRIME", "445 AO-MinVolts": "0.0000", "PIEZO-Name": "Stage", "445 AO-Description": "NI DAC", "PRIME-CircularBufferFrameCount": "46", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "520 AO-Volts": "0.0000", "528 Shutter-TriggerSampleRate": "100000", "XPositionUm": 23269.100000000002, "OpticalPath-State": "4", "PRIME-PP 3 THRESHOLD": "80", "PRIME-PVCAM Version": "3.7.1", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Gain": "1-HDR", "640 Shutter-TriggerSequenceLength": "1000", "528 AO-SupportsTriggering": "0", "445 AO-TriggerInputLine": "/Dev1/PFI0", "OpticalPath-Name": "OpticalPath", "PRIME-PP 4 ENABLED": "Yes", "PRIME-PVCAM Adapter Version": "1.3.29", "COM3-Name": "COM3", "405 AO-MaxVolts": "5.0000", "520 Shutter-Block voltage": "false", "PRIME-Timing-ClearingTimeNs": "49344000.0000", "Core-Focus": "PIEZO", "528 AO-Block voltage": "false", "TE2000-Description": "Nikon TE2000 hub", "528 AO-MinVolts": "0.0000", "COM4-Parity": "None", "Summary": { "Slices": 11, "ChContrastMin": [ 0, 0, 0, 0, 0, 0 ], "PixelAspect": 1, "SlicesFirst": true, "Interval_ms": 0, "Time": "2019-12-17 03:59:33 -0500", "Prefix": "seq_7", "InitialPositionList": [ { "Label": "Pos0", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [149.91], "Focus": [16657.7], "XYStage": [ 71629.7, 19152.9 ] }, "GridColumnIndex": 0 }, { "Label": "Pos1", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [190.82], "Focus": [16485.4], "XYStage": [ 65702.3, 19396.600000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos2", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [194.69], "Focus": [16446.8], "XYStage": [ 59880.5, 19007.8 ] }, "GridColumnIndex": 0 }, { "Label": "Pos3", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [145.04], "Focus": [16446.6], "XYStage": [ 53616.9, 18818.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos4", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [169.57500000000002], "Focus": [16446.5], "XYStage": [ 47662.8, 18817.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos5", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [131.805], "Focus": [16446.4], "XYStage": [ 41918.8, 18298.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos6", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [113.145], "Focus": [16446.5], "XYStage": [ 36129.9, 18895.8 ] }, "GridColumnIndex": 0 }, { "Label": "Pos7", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [141.005], "Focus": [16446.6], "XYStage": [ 30299.100000000002, 18895.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos8", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [140.97], "Focus": [16446.5], "XYStage": [ 23952.100000000002, 19156 ] }, "GridColumnIndex": 0 }, { "Label": "Pos9", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [184.57500000000002], "Focus": [16677.9], "XYStage": [ 72866.8, -14809.300000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos10", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [167.95000000000002], "Focus": [16557.9], "XYStage": [ 66515.6, -14371.300000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos11", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [168], "Focus": [16618.9], "XYStage": [ 59724.3, -13964.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos12", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [203.205], "Focus": [16779.6], "XYStage": [ 53862.3, -13494.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos13", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [203.20000000000002], "Focus": [16749], "XYStage": [ 48108.3, -13805.400000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos14", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [203.19], "Focus": [16675.9], "XYStage": [ 41645, -13804.900000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos15", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [203.21], "Focus": [16675.7], "XYStage": [ 35352.6, -13805.6 ] }, "GridColumnIndex": 0 }, { "Label": "Pos16", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [203.225], "Focus": [16675.8], "XYStage": [ 29298, -13447.400000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos17", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [203.215], "Focus": [16675.8], "XYStage": [ 23269.100000000002, -13012.300000000001 ] }, "GridColumnIndex": 0 } ], "z-step_um": 10, "GridRow": 0, "BitDepth": 16, "Depth": 2, "Channels": 6, "Source": "Micro-Manager", "ChColors": [ -16711681, -256, -65281, -1, -1, -16776961 ], "MetadataVersion": 10, "KeepShutterOpenChannels": true, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536, 65536 ], "CustomIntervals_ms": [], "Height": 1200, "MicroManagerVersion": "1.4.23 20180219", "GridColumn": 0, "UUID": "3ad8801b-5699-40d6-813b-4ad840863e38", "PixelType": "GRAY16", "Width": 1200, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\Yan\\20191216-YC113-S11S12-miseq", "Frames": 1, "Comment": "", "UserName": "Nikon", "PixelSize_um": 1.1, "ROI": [ 0, 0, 1200, 1200 ], "Date": "2019-12-17", "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC", "P-DAPI-high" ], "KeepShutterOpenSlices": true, "IJType": 1, "ComputerName": "ZadorConfocal2", "Positions": 18, "PositionIndex": 17, "CameraTimeout": 20000, "TimeFirst": false }, "640 AO-Name": "AnalogIO", "Vincent-D3-Command": "Close" }, "FrameKey-0-1-2": { "COM4-StopBits": "1", "528 AO-MaxVolts": "5.0000", "XLIGHT Excitation Wheel-State": "1", "470 Shutter-Name": "AnalogIO", "Core-Shutter": "555", "555 AO-Demo": "No", "XLIGHT Dichroic-State": "2", "XYStage-StepSize-Y": "0.1000", "ZPositionUm": 173.215, "XYStage-StepSize-X": "0.1000", "PRIME-FirmwareVersion": "20.28", "PRIME-ClearCycles": "2", "520-Name": "DA Shutter", "405 Shutter-Name": "AnalogIO", "PIEZO-StepSize": "0.0050", "PRIME-ClearMode": "Pre-Exposure", "470 AO-MinVolts": "0.0000", "405 AO-SupportsTriggering": "1", "CameraChannelIndex": 0, "XYStage-StartSpeed": "500.0000", "COM9-StopBits": "2", "405 AO-Description": "NI DAC", "COM4-DelayBetweenCharsMs": "0.0000", "445 AO-Block voltage": "false", "520 AO-TriggerInputLine": "/Dev1/PFI0", "555 AO-Description": "NI DAC", "520 AO-TriggerSampleRate": "100000", "COM4-Verbose": "1", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM4-Name": "COM4", "405 Shutter-Volts": "0.0000", "Vincent-D3-Port": "COM1", "PRIME-PP 4 MIN ADU AFFECTED": "200", "470 Shutter-MinVolts": "0.0000", "PRIME-PP 3 ENABLED": "Yes", "470 AO-Description": "NI DAC", "PRIME-Description": "PVCAM API device adapter", "520 Shutter-MaxVolts": "5.0000", "520 Shutter-Volts": "0.0000", "528 AO-Sequenceable": "1", "PRIME-Binning": "1x1", "555 Shutter-MaxVolts": "5.0000", "528 Shutter-TriggerSequenceLength": "1000", "405 Shutter-Block voltage": "false", "XLIGHT Spin motor-State": "1", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "Vincent-D3-Time to open (ms)": "35.0000", "Vincent-D3-Time to close (ms)": "35.0000", "445 AO-TriggerSampleRate": "100000", "640 Shutter-IOChannel": "Dev1/ao14", "555 AO-Sequenceable": "1", "520 AO-Name": "AnalogIO", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "470 AO-Block voltage": "false", "COM1-Verbose": "1", "COM9-AnswerTimeout": "10000.0000", "528 Shutter-Name": "AnalogIO", "PRIME-Trigger Timeout (secs)": "10", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "NextFrame": 0, "470 AO-TriggerSequenceLength": "1000", "PRIME-TransposeXY": "0", "445 AO-TriggerSequenceLength": "1000", "COM1-Name": "COM1", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "COM9-DataBits": "8", "COM1-AnswerTimeout": "500.0000", "Camera": "", "TE2000-Version": "V2.50-V2.50-V2.10", "528 Shutter-Volts": "0.0000", "XLIGHT Hub-Name": "XLIGHT Hub", "PIEZO-Description": "Ludl stage driver adapter", "PositionName": "Pos17", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSampleRate": "100000", "PRIME-FanSpeedSetpoint": "High", "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "405 AO-TriggerSampleRate": "100000", "470 Shutter-Block voltage": "false", "470-State": "0", "COM3-Parity": "None", "405 AO-Volts": "0.0000", "PRIME-TransposeMirrorY": "0", "PRIME-ChipName": "GS144BSI", "470 AO-Volts": "0.0000", "PRIME-TransposeMirrorX": "0", "445 Shutter-SupportsTriggering": "0", "470 Shutter-SupportsTriggering": "0", "XLIGHT Hub-Description": "XLIGHT hub", "PRIME-PreampOffLimit": "10000", "470 AO-Sequenceable": "1", "528 Shutter-MaxVolts": "5.0000", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "Core-XYStage": "XYStage", "470 AO-TriggerSampleRate": "100000", "PRIME-Timing-PreTriggerDelayNs": "24672000.0000", "528-DA Device": "528 Shutter", "Focus-Description": "Nikon TE2000 focus adapter", "FilterBlock-State": "5", "520 AO-Demo": "No", "COM3-DelayBetweenCharsMs": "0.0000", "470 Shutter-Volts": "0.0000", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "PRIME-PP 2 THRESHOLD": "125", "PRIME-PP 4 THRESHOLD": "75", "XYStage-Acceleration": "4.0000", "470 Shutter-Demo": "No", "PRIME-Name": "PMPCIECam00", "640-DA Device": "640 Shutter", "405 AO-Block voltage": "false", "ElapsedTime-ms": 724078, "PRIME-Offset": "100", "445 AO-IOChannel": "Dev1/ao1", "640 AO-MaxVolts": "5.0000", "PositionIndex": 17, "PRIME-PP 1 ENABLED": "Yes", "COM1-BaudRate": "9600", "640-State": "0", "COM3-Verbose": "1", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "LudlController-TransmissionDelay": "0", "PRIME-FullWellCapacity": "62000", "PRIME-SMARTStreamingEnabled": "No", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "640 Shutter-Description": "NI DAC", "528-Description": "DA device that is used as a shutter", "COM4-Description": "Serial port driver (boost:asio)", "405 Shutter-Description": "NI DAC", "TE2000-Name": "TE2000", "528 Shutter-Description": "NI DAC", "COM9-Description": "Serial port driver (boost:asio)", "LudlController-Name": "LudlController", "445 Shutter-TriggerSequenceLength": "1000", "PRIME-SerialNumber": "A18B203008", "445 AO-Volts": "0.0000", "PRIME-X-dimension": "1200", "PRIME-ShutterCloseDelay": "0", "XLIGHT Disk slider-State": "2", "Position": "Default", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 AO-IOChannel": "Dev1/ao5", "520 AO-IOChannel": "Dev1/ao4", "528 AO-TriggerSequenceLength": "1000", "405 Shutter-MaxVolts": "5.0000", "470-DA Device": "470 Shutter", "PRIME-FTCapable": "No", "Core-ChannelGroup": "Channels", "555 AO-SupportsTriggering": "0", "PRIME-Y-dimension": "1200", "Frame": 0, "555 Shutter-Sequenceable": "1", "PRIME-Timing-ExposureTimeNs": "499998000.0000", "Core-ImageProcessor": "", "528-State": "0", "520 AO-Block voltage": "false", "XLIGHT Excitation Wheel-Label": "QUAD", "640 Shutter-Demo": "No", "COM3-StopBits": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "520 Shutter-IOChannel": "Dev1/ao11", "Width": 1200, "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-SupportsTriggering": "0", "640 AO-TriggerSampleRate": "100000", "520-Description": "DA device that is used as a shutter", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "405 Shutter-Sequenceable": "1", "405 Shutter-SupportsTriggering": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-TriggerSampleRate": "100000", "640 Shutter-MaxVolts": "5.0000", "555 AO-MaxVolts": "5.0000", "520 AO-TriggerSequenceLength": "1000", "COM3-BaudRate": "9600", "COM9-BaudRate": "9600", "Vincent-D3-Channel #": "Ch. #1", "PRIME-PMode": "Normal", "OpticalPath-Label": "Left(100)", "BitDepth": 16, "555 AO-TriggerSequenceLength": "1000", "445 Shutter-Description": "NI DAC", "405 AO-Sequenceable": "1", "PRIME-AcquisitionMethod": "Callbacks", "555 AO-Name": "AnalogIO", "PRIME-ShutterMode": "Pre-Exposure", "COM3-Handshaking": "Off", "445 AO-SupportsTriggering": "0", "ROI": "0-0-1200-1200", "COM9-Verbose": "1", "PRIME-Color": "OFF", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "PRIME-ShutterOpenDelay": "0", "COM1-Parity": "None", "Slice": 2, "SlicePosition": -30, "COM9-DelayBetweenCharsMs": "0.0000", "555-Name": "DA Shutter", "PIEZO-LudlSingleAxisName": "B", "XLIGHT Emission Wheel-Label": "585/11(T)", "640 Shutter-Name": "AnalogIO", "640 AO-Sequenceable": "1", "520 Shutter-Demo": "No", "528 Shutter-Demo": "No", "470-Name": "DA Shutter", "520-DA Device": "520 Shutter", "XYStage-Description": "Ludl XY stage driver adapter", "445 Shutter-MinVolts": "0.0000", "528 AO-TriggerSampleRate": "100000", "405 AO-MinVolts": "0.0000", "405 Shutter-TriggerSequenceLength": "1000", "Height": 1200, "555 AO-Block voltage": "false", "640 Shutter-Volts": "0.0000", "PixelType": "GRAY16", "445 Shutter-Block voltage": "false", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "520 Shutter-TriggerSequenceLength": "1000", "COM9-Name": "COM9", "Core-SLM": "", "555 AO-Volts": "4.5000", "555 Shutter-Block voltage": "false", "XYStage-Speed": "1800.0000", "520 AO-MaxVolts": "5.0000", "528 Shutter-SupportsTriggering": "0", "COM1-Description": "Serial port driver (boost:asio)", "PRIME-MetadataEnabled": "No", "520 Shutter-MinVolts": "0.0000", "470 Shutter-Sequenceable": "1", "PRIME-PreampDelay": "0", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-MinVolts": "0.0000", "ChannelIndex": 1, "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Core-AutoShutter": "1", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "Exposure-ms": 160, "PRIME-PP 2 ENABLED": "Yes", "640 Shutter-TriggerSampleRate": "100000", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "470 Shutter-IOChannel": "Dev1/ao10", "405 AO-Demo": "No", "PixelSizeUm": 1.1, "470 AO-Demo": "No", "470 Shutter-MaxVolts": "5.0000", "Vincent-D3-Name": "Vincent-D3", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FileName": "img_000000000_Il-T_002.tif", "LudlController-Reset": "Operate", "PRIME-Exposure": "160.00", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "445-Name": "DA Shutter", "555 Shutter-Name": "AnalogIO", "520 Shutter-TriggerSampleRate": "100000", "445 Shutter-IOChannel": "Dev1/ao9", "PRIME-TransposeCorrection": "0", "XYStage-StepSize": "0.1000", "445-Description": "DA device that is used as a shutter", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 AO-IOChannel": "Dev1/ao7", "520 Shutter-Name": "AnalogIO", "COM3-DataBits": "8", "640 AO-Volts": "0.0000", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "555 Shutter-IOChannel": "Dev1/ao13", "WaitInterval": null, "520 Shutter-Sequenceable": "1", "YPositionUm": -13012.300000000001, "445-State": "0", "445-DA Device": "445 Shutter", "PRIME-CircularBufferAutoSize": "ON", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "555 AO-TriggerSampleRate": "100000", "445 Shutter-Volts": "0.0000", "XLIGHT Hub-Port": "COM3", "Core-AutoFocus": "", "COM4-Handshaking": "Off", "528 Shutter-MinVolts": "0.0000", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "445 Shutter-Name": "AnalogIO", "528 Shutter-Sequenceable": "1", "XYStage-TransposeMirrorX": "0", "XYStage-TransposeMirrorY": "0", "PRIME-ReadoutRate": "100MHz 16bit", "XYStage-Name": "Stage", "640 AO-SupportsTriggering": "0", "640-Name": "DA Shutter", "640 Shutter-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "640 AO-Block voltage": "false", "445 Shutter-Sequenceable": "1", "470 AO-MaxVolts": "5.0000", "COM4-BaudRate": "9600", "470-Description": "DA device that is used as a shutter", "520 Shutter-Description": "NI DAC", "PRIME-CCDTemperature": "-14.9200", "Time": "2019-12-17 03:59:41 -0500", "640 AO-TriggerSequenceLength": "1000", "470 AO-Name": "AnalogIO", "XLIGHT Disk slider-Label": "Disk Pos 40um", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "Binning": "1x1", "TE2000-ModelType": "1", "COM3-AnswerTimeout": "500.0000", "555 Shutter-SupportsTriggering": "0", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "TE2000-Port": "COM4", "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Description": "NI DAC", "COM4-AnswerTimeout": "500.0000", "COM1-DelayBetweenCharsMs": "0.0000", "FilterBlock-Label": "Confocal", "445 AO-Name": "AnalogIO", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-Timing-ReadoutTimeNs": "24672000.0000", "528 AO-Demo": "No", "520 AO-Sequenceable": "1", "555-Description": "DA device that is used as a shutter", "405-DA Device": "405 Shutter", "405 AO-TriggerSequenceLength": "1000", "640 AO-MinVolts": "0.0000", "640 Shutter-Block voltage": "false", "FrameIndex": 0, "PRIME-Port": "CMOS", "555 AO-MinVolts": "0.0000", "COM3-Description": "Serial port driver (boost:asio)", "528 AO-Description": "NI DAC", "555 AO-IOChannel": "Dev1/ao6", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "555 AO-TriggerInputLine": "/Dev1/PFI0", "PRIME-ActualInterval-ms": "84.6200", "LudlController-Port": "COM9", "445 Shutter-Demo": "No", "PRIME-Trigger-Expose Out-Mux": "1", "SliceIndex": 2, "PRIME-CCDTemperatureSetPoint": "-15.0000", "405 Shutter-MinVolts": "0.0000", "PRIME-ExposeOutMode": "First Row", "AxisPositions": null, "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Volts": "5.0000", "528-Name": "DA Shutter", "555-State": "1", "XLIGHT Dichroic-Label": "QUAD", "445 AO-Sequenceable": "1", "405-Name": "DA Shutter", "405 AO-IOChannel": "Dev1/ao0", "Core-Camera": "PRIME", "Focus-Name": "Focus", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "405 Shutter-IOChannel": "Dev1/ao8", "640 AO-Demo": "No", "640 Shutter-SupportsTriggering": "0", "445 AO-Demo": "No", "555-DA Device": "555 Shutter", "470 Shutter-Description": "NI DAC", "528 AO-Volts": "0.0000", "COM1-DataBits": "8", "Vincent-D3-Address": "x", "520 AO-SupportsTriggering": "0", "405-Description": "DA device that is used as a shutter", "640 Shutter-MinVolts": "0.0000", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "405 AO-Name": "AnalogIO", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "640 AO-TriggerInputLine": "/Dev1/PFI0", "405 AO-TriggerInputLine": "/Dev1/PFI0", "640-Description": "DA device that is used as a shutter", "445 Shutter-TriggerSampleRate": "100000", "470 Shutter-TriggerSequenceLength": "1000", "COM1-StopBits": "1", "520 Shutter-SupportsTriggering": "0", "405-State": "0", "Core-Initialize": "1", "XLIGHT Emission Wheel-State": "3", "520 AO-Description": "NI DAC", "528 AO-TriggerInputLine": "/Dev1/PFI0", "445 Shutter-MaxVolts": "5.0000", "COM9-Handshaking": "Off", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "XLIGHT Spin motor-Label": "On (spinning)", "PRIME-PP 5 ENABLED": "No", "UUID": "ef1084f7-9cfa-420a-901b-9b848ac5121f", "PRIME-TriggerMode": "Internal Trigger", "COM1-Handshaking": "Off", "PRIME-Timing-PostTriggerDelayNs": "49344000.0000", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "Focus-Position": "16732.9000", "528 Shutter-Block voltage": "false", "Channel": "Il-T", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "555 Shutter-Demo": "No", "405 Shutter-Demo": "No", "528 AO-Name": "AnalogIO", "520-State": "0", "Core-Galvo": "", "405 Shutter-TriggerSampleRate": "100000", "Focus-FocusStepSize_(nm)": "100", "PRIME-PixelType": "16bit", "COM4-DataBits": "8", "470 AO-IOChannel": "Dev1/ao2", "520 AO-MinVolts": "0.0000", "COM9-Parity": "None", "PRIME-CircularBufferEnabled": "ON", "PRIME-PP 6 Reset": "No", "640 AO-Description": "NI DAC", "Source": "PRIME", "445 AO-MinVolts": "0.0000", "PIEZO-Name": "Stage", "445 AO-Description": "NI DAC", "PRIME-CircularBufferFrameCount": "46", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "520 AO-Volts": "0.0000", "528 Shutter-TriggerSampleRate": "100000", "XPositionUm": 23269.100000000002, "OpticalPath-State": "4", "PRIME-PP 3 THRESHOLD": "80", "PRIME-PVCAM Version": "3.7.1", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Gain": "1-HDR", "640 Shutter-TriggerSequenceLength": "1000", "528 AO-SupportsTriggering": "0", "445 AO-TriggerInputLine": "/Dev1/PFI0", "OpticalPath-Name": "OpticalPath", "PRIME-PP 4 ENABLED": "Yes", "PRIME-PVCAM Adapter Version": "1.3.29", "COM3-Name": "COM3", "405 AO-MaxVolts": "5.0000", "520 Shutter-Block voltage": "false", "PRIME-Timing-ClearingTimeNs": "49344000.0000", "Core-Focus": "PIEZO", "528 AO-Block voltage": "false", "TE2000-Description": "Nikon TE2000 hub", "528 AO-MinVolts": "0.0000", "COM4-Parity": "None", "Summary": { "Slices": 11, "ChContrastMin": [ 0, 0, 0, 0, 0, 0 ], "PixelAspect": 1, "SlicesFirst": true, "Interval_ms": 0, "Time": "2019-12-17 03:59:33 -0500", "Prefix": "seq_7", "InitialPositionList": [ { "Label": "Pos0", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [149.91], "Focus": [16657.7], "XYStage": [ 71629.7, 19152.9 ] }, "GridColumnIndex": 0 }, { "Label": "Pos1", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [190.82], "Focus": [16485.4], "XYStage": [ 65702.3, 19396.600000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos2", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [194.69], "Focus": [16446.8], "XYStage": [ 59880.5, 19007.8 ] }, "GridColumnIndex": 0 }, { "Label": "Pos3", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [145.04], "Focus": [16446.6], "XYStage": [ 53616.9, 18818.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos4", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [169.57500000000002], "Focus": [16446.5], "XYStage": [ 47662.8, 18817.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos5", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [131.805], "Focus": [16446.4], "XYStage": [ 41918.8, 18298.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos6", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [113.145], "Focus": [16446.5], "XYStage": [ 36129.9, 18895.8 ] }, "GridColumnIndex": 0 }, { "Label": "Pos7", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [141.005], "Focus": [16446.6], "XYStage": [ 30299.100000000002, 18895.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos8", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [140.97], "Focus": [16446.5], "XYStage": [ 23952.100000000002, 19156 ] }, "GridColumnIndex": 0 }, { "Label": "Pos9", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [184.57500000000002], "Focus": [16677.9], "XYStage": [ 72866.8, -14809.300000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos10", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [167.95000000000002], "Focus": [16557.9], "XYStage": [ 66515.6, -14371.300000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos11", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [168], "Focus": [16618.9], "XYStage": [ 59724.3, -13964.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos12", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [203.205], "Focus": [16779.6], "XYStage": [ 53862.3, -13494.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos13", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [203.20000000000002], "Focus": [16749], "XYStage": [ 48108.3, -13805.400000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos14", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [203.19], "Focus": [16675.9], "XYStage": [ 41645, -13804.900000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos15", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [203.21], "Focus": [16675.7], "XYStage": [ 35352.6, -13805.6 ] }, "GridColumnIndex": 0 }, { "Label": "Pos16", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [203.225], "Focus": [16675.8], "XYStage": [ 29298, -13447.400000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos17", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [203.215], "Focus": [16675.8], "XYStage": [ 23269.100000000002, -13012.300000000001 ] }, "GridColumnIndex": 0 } ], "z-step_um": 10, "GridRow": 0, "BitDepth": 16, "Depth": 2, "Channels": 6, "Source": "Micro-Manager", "ChColors": [ -16711681, -256, -65281, -1, -1, -16776961 ], "MetadataVersion": 10, "KeepShutterOpenChannels": true, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536, 65536 ], "CustomIntervals_ms": [], "Height": 1200, "MicroManagerVersion": "1.4.23 20180219", "GridColumn": 0, "UUID": "3ad8801b-5699-40d6-813b-4ad840863e38", "PixelType": "GRAY16", "Width": 1200, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\Yan\\20191216-YC113-S11S12-miseq", "Frames": 1, "Comment": "", "UserName": "Nikon", "PixelSize_um": 1.1, "ROI": [ 0, 0, 1200, 1200 ], "Date": "2019-12-17", "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC", "P-DAPI-high" ], "KeepShutterOpenSlices": true, "IJType": 1, "ComputerName": "ZadorConfocal2", "Positions": 18, "PositionIndex": 17, "CameraTimeout": 20000, "TimeFirst": false }, "640 AO-Name": "AnalogIO", "Vincent-D3-Command": "Close" }, "FrameKey-0-1-3": { "COM4-StopBits": "1", "528 AO-MaxVolts": "5.0000", "XLIGHT Excitation Wheel-State": "1", "470 Shutter-Name": "AnalogIO", "Core-Shutter": "555", "555 AO-Demo": "No", "XLIGHT Dichroic-State": "2", "XYStage-StepSize-Y": "0.1000", "ZPositionUm": 183.215, "XYStage-StepSize-X": "0.1000", "PRIME-FirmwareVersion": "20.28", "PRIME-ClearCycles": "2", "520-Name": "DA Shutter", "405 Shutter-Name": "AnalogIO", "PIEZO-StepSize": "0.0050", "PRIME-ClearMode": "Pre-Exposure", "470 AO-MinVolts": "0.0000", "405 AO-SupportsTriggering": "1", "CameraChannelIndex": 0, "XYStage-StartSpeed": "500.0000", "COM9-StopBits": "2", "405 AO-Description": "NI DAC", "COM4-DelayBetweenCharsMs": "0.0000", "445 AO-Block voltage": "false", "520 AO-TriggerInputLine": "/Dev1/PFI0", "555 AO-Description": "NI DAC", "520 AO-TriggerSampleRate": "100000", "COM4-Verbose": "1", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM4-Name": "COM4", "405 Shutter-Volts": "0.0000", "Vincent-D3-Port": "COM1", "PRIME-PP 4 MIN ADU AFFECTED": "200", "470 Shutter-MinVolts": "0.0000", "PRIME-PP 3 ENABLED": "Yes", "470 AO-Description": "NI DAC", "PRIME-Description": "PVCAM API device adapter", "520 Shutter-MaxVolts": "5.0000", "520 Shutter-Volts": "0.0000", "528 AO-Sequenceable": "1", "PRIME-Binning": "1x1", "555 Shutter-MaxVolts": "5.0000", "528 Shutter-TriggerSequenceLength": "1000", "405 Shutter-Block voltage": "false", "XLIGHT Spin motor-State": "1", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "Vincent-D3-Time to open (ms)": "35.0000", "Vincent-D3-Time to close (ms)": "35.0000", "445 AO-TriggerSampleRate": "100000", "640 Shutter-IOChannel": "Dev1/ao14", "555 AO-Sequenceable": "1", "520 AO-Name": "AnalogIO", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "470 AO-Block voltage": "false", "COM1-Verbose": "1", "COM9-AnswerTimeout": "10000.0000", "528 Shutter-Name": "AnalogIO", "PRIME-Trigger Timeout (secs)": "10", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "NextFrame": 0, "470 AO-TriggerSequenceLength": "1000", "PRIME-TransposeXY": "0", "445 AO-TriggerSequenceLength": "1000", "COM1-Name": "COM1", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "COM9-DataBits": "8", "COM1-AnswerTimeout": "500.0000", "Camera": "", "TE2000-Version": "V2.50-V2.50-V2.10", "528 Shutter-Volts": "0.0000", "XLIGHT Hub-Name": "XLIGHT Hub", "PIEZO-Description": "Ludl stage driver adapter", "PositionName": "Pos17", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSampleRate": "100000", "PRIME-FanSpeedSetpoint": "High", "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "405 AO-TriggerSampleRate": "100000", "470 Shutter-Block voltage": "false", "470-State": "0", "COM3-Parity": "None", "405 AO-Volts": "0.0000", "PRIME-TransposeMirrorY": "0", "PRIME-ChipName": "GS144BSI", "470 AO-Volts": "0.0000", "PRIME-TransposeMirrorX": "0", "445 Shutter-SupportsTriggering": "0", "470 Shutter-SupportsTriggering": "0", "XLIGHT Hub-Description": "XLIGHT hub", "PRIME-PreampOffLimit": "10000", "470 AO-Sequenceable": "1", "528 Shutter-MaxVolts": "5.0000", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "Core-XYStage": "XYStage", "470 AO-TriggerSampleRate": "100000", "PRIME-Timing-PreTriggerDelayNs": "24672000.0000", "528-DA Device": "528 Shutter", "Focus-Description": "Nikon TE2000 focus adapter", "FilterBlock-State": "5", "520 AO-Demo": "No", "COM3-DelayBetweenCharsMs": "0.0000", "470 Shutter-Volts": "0.0000", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "PRIME-PP 2 THRESHOLD": "125", "PRIME-PP 4 THRESHOLD": "75", "XYStage-Acceleration": "4.0000", "470 Shutter-Demo": "No", "PRIME-Name": "PMPCIECam00", "640-DA Device": "640 Shutter", "405 AO-Block voltage": "false", "ElapsedTime-ms": 724479, "PRIME-Offset": "100", "445 AO-IOChannel": "Dev1/ao1", "640 AO-MaxVolts": "5.0000", "PositionIndex": 17, "PRIME-PP 1 ENABLED": "Yes", "COM1-BaudRate": "9600", "640-State": "0", "COM3-Verbose": "1", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "LudlController-TransmissionDelay": "0", "PRIME-FullWellCapacity": "62000", "PRIME-SMARTStreamingEnabled": "No", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "640 Shutter-Description": "NI DAC", "528-Description": "DA device that is used as a shutter", "COM4-Description": "Serial port driver (boost:asio)", "405 Shutter-Description": "NI DAC", "TE2000-Name": "TE2000", "528 Shutter-Description": "NI DAC", "COM9-Description": "Serial port driver (boost:asio)", "LudlController-Name": "LudlController", "445 Shutter-TriggerSequenceLength": "1000", "PRIME-SerialNumber": "A18B203008", "445 AO-Volts": "0.0000", "PRIME-X-dimension": "1200", "PRIME-ShutterCloseDelay": "0", "XLIGHT Disk slider-State": "2", "Position": "Default", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 AO-IOChannel": "Dev1/ao5", "520 AO-IOChannel": "Dev1/ao4", "528 AO-TriggerSequenceLength": "1000", "405 Shutter-MaxVolts": "5.0000", "470-DA Device": "470 Shutter", "PRIME-FTCapable": "No", "Core-ChannelGroup": "Channels", "555 AO-SupportsTriggering": "0", "PRIME-Y-dimension": "1200", "Frame": 0, "555 Shutter-Sequenceable": "1", "PRIME-Timing-ExposureTimeNs": "499998000.0000", "Core-ImageProcessor": "", "528-State": "0", "520 AO-Block voltage": "false", "XLIGHT Excitation Wheel-Label": "QUAD", "640 Shutter-Demo": "No", "COM3-StopBits": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "520 Shutter-IOChannel": "Dev1/ao11", "Width": 1200, "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-SupportsTriggering": "0", "640 AO-TriggerSampleRate": "100000", "520-Description": "DA device that is used as a shutter", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "405 Shutter-Sequenceable": "1", "405 Shutter-SupportsTriggering": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-TriggerSampleRate": "100000", "640 Shutter-MaxVolts": "5.0000", "555 AO-MaxVolts": "5.0000", "520 AO-TriggerSequenceLength": "1000", "COM3-BaudRate": "9600", "COM9-BaudRate": "9600", "Vincent-D3-Channel #": "Ch. #1", "PRIME-PMode": "Normal", "OpticalPath-Label": "Left(100)", "BitDepth": 16, "555 AO-TriggerSequenceLength": "1000", "445 Shutter-Description": "NI DAC", "405 AO-Sequenceable": "1", "PRIME-AcquisitionMethod": "Callbacks", "555 AO-Name": "AnalogIO", "PRIME-ShutterMode": "Pre-Exposure", "COM3-Handshaking": "Off", "445 AO-SupportsTriggering": "0", "ROI": "0-0-1200-1200", "COM9-Verbose": "1", "PRIME-Color": "OFF", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "PRIME-ShutterOpenDelay": "0", "COM1-Parity": "None", "Slice": 3, "SlicePosition": -20, "COM9-DelayBetweenCharsMs": "0.0000", "555-Name": "DA Shutter", "PIEZO-LudlSingleAxisName": "B", "XLIGHT Emission Wheel-Label": "585/11(T)", "640 Shutter-Name": "AnalogIO", "640 AO-Sequenceable": "1", "520 Shutter-Demo": "No", "528 Shutter-Demo": "No", "470-Name": "DA Shutter", "520-DA Device": "520 Shutter", "XYStage-Description": "Ludl XY stage driver adapter", "445 Shutter-MinVolts": "0.0000", "528 AO-TriggerSampleRate": "100000", "405 AO-MinVolts": "0.0000", "405 Shutter-TriggerSequenceLength": "1000", "Height": 1200, "555 AO-Block voltage": "false", "640 Shutter-Volts": "0.0000", "PixelType": "GRAY16", "445 Shutter-Block voltage": "false", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "520 Shutter-TriggerSequenceLength": "1000", "COM9-Name": "COM9", "Core-SLM": "", "555 AO-Volts": "4.5000", "555 Shutter-Block voltage": "false", "XYStage-Speed": "1800.0000", "520 AO-MaxVolts": "5.0000", "528 Shutter-SupportsTriggering": "0", "COM1-Description": "Serial port driver (boost:asio)", "PRIME-MetadataEnabled": "No", "520 Shutter-MinVolts": "0.0000", "470 Shutter-Sequenceable": "1", "PRIME-PreampDelay": "0", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-MinVolts": "0.0000", "ChannelIndex": 1, "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Core-AutoShutter": "1", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "Exposure-ms": 160, "PRIME-PP 2 ENABLED": "Yes", "640 Shutter-TriggerSampleRate": "100000", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "470 Shutter-IOChannel": "Dev1/ao10", "405 AO-Demo": "No", "PixelSizeUm": 1.1, "470 AO-Demo": "No", "470 Shutter-MaxVolts": "5.0000", "Vincent-D3-Name": "Vincent-D3", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FileName": "img_000000000_Il-T_003.tif", "LudlController-Reset": "Operate", "PRIME-Exposure": "160.00", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "445-Name": "DA Shutter", "555 Shutter-Name": "AnalogIO", "520 Shutter-TriggerSampleRate": "100000", "445 Shutter-IOChannel": "Dev1/ao9", "PRIME-TransposeCorrection": "0", "XYStage-StepSize": "0.1000", "445-Description": "DA device that is used as a shutter", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 AO-IOChannel": "Dev1/ao7", "520 Shutter-Name": "AnalogIO", "COM3-DataBits": "8", "640 AO-Volts": "0.0000", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "555 Shutter-IOChannel": "Dev1/ao13", "WaitInterval": null, "520 Shutter-Sequenceable": "1", "YPositionUm": -13012.300000000001, "445-State": "0", "445-DA Device": "445 Shutter", "PRIME-CircularBufferAutoSize": "ON", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "555 AO-TriggerSampleRate": "100000", "445 Shutter-Volts": "0.0000", "XLIGHT Hub-Port": "COM3", "Core-AutoFocus": "", "COM4-Handshaking": "Off", "528 Shutter-MinVolts": "0.0000", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "445 Shutter-Name": "AnalogIO", "528 Shutter-Sequenceable": "1", "XYStage-TransposeMirrorX": "0", "XYStage-TransposeMirrorY": "0", "PRIME-ReadoutRate": "100MHz 16bit", "XYStage-Name": "Stage", "640 AO-SupportsTriggering": "0", "640-Name": "DA Shutter", "640 Shutter-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "640 AO-Block voltage": "false", "445 Shutter-Sequenceable": "1", "470 AO-MaxVolts": "5.0000", "COM4-BaudRate": "9600", "470-Description": "DA device that is used as a shutter", "520 Shutter-Description": "NI DAC", "PRIME-CCDTemperature": "-14.9200", "Time": "2019-12-17 03:59:41 -0500", "640 AO-TriggerSequenceLength": "1000", "470 AO-Name": "AnalogIO", "XLIGHT Disk slider-Label": "Disk Pos 40um", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "Binning": "1x1", "TE2000-ModelType": "1", "COM3-AnswerTimeout": "500.0000", "555 Shutter-SupportsTriggering": "0", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "TE2000-Port": "COM4", "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Description": "NI DAC", "COM4-AnswerTimeout": "500.0000", "COM1-DelayBetweenCharsMs": "0.0000", "FilterBlock-Label": "Confocal", "445 AO-Name": "AnalogIO", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-Timing-ReadoutTimeNs": "24672000.0000", "528 AO-Demo": "No", "520 AO-Sequenceable": "1", "555-Description": "DA device that is used as a shutter", "405-DA Device": "405 Shutter", "405 AO-TriggerSequenceLength": "1000", "640 AO-MinVolts": "0.0000", "640 Shutter-Block voltage": "false", "FrameIndex": 0, "PRIME-Port": "CMOS", "555 AO-MinVolts": "0.0000", "COM3-Description": "Serial port driver (boost:asio)", "528 AO-Description": "NI DAC", "555 AO-IOChannel": "Dev1/ao6", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "555 AO-TriggerInputLine": "/Dev1/PFI0", "PRIME-ActualInterval-ms": "84.6200", "LudlController-Port": "COM9", "445 Shutter-Demo": "No", "PRIME-Trigger-Expose Out-Mux": "1", "SliceIndex": 3, "PRIME-CCDTemperatureSetPoint": "-15.0000", "405 Shutter-MinVolts": "0.0000", "PRIME-ExposeOutMode": "First Row", "AxisPositions": null, "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Volts": "5.0000", "528-Name": "DA Shutter", "555-State": "1", "XLIGHT Dichroic-Label": "QUAD", "445 AO-Sequenceable": "1", "405-Name": "DA Shutter", "405 AO-IOChannel": "Dev1/ao0", "Core-Camera": "PRIME", "Focus-Name": "Focus", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "405 Shutter-IOChannel": "Dev1/ao8", "640 AO-Demo": "No", "640 Shutter-SupportsTriggering": "0", "445 AO-Demo": "No", "555-DA Device": "555 Shutter", "470 Shutter-Description": "NI DAC", "528 AO-Volts": "0.0000", "COM1-DataBits": "8", "Vincent-D3-Address": "x", "520 AO-SupportsTriggering": "0", "405-Description": "DA device that is used as a shutter", "640 Shutter-MinVolts": "0.0000", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "405 AO-Name": "AnalogIO", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "640 AO-TriggerInputLine": "/Dev1/PFI0", "405 AO-TriggerInputLine": "/Dev1/PFI0", "640-Description": "DA device that is used as a shutter", "445 Shutter-TriggerSampleRate": "100000", "470 Shutter-TriggerSequenceLength": "1000", "COM1-StopBits": "1", "520 Shutter-SupportsTriggering": "0", "405-State": "0", "Core-Initialize": "1", "XLIGHT Emission Wheel-State": "3", "520 AO-Description": "NI DAC", "528 AO-TriggerInputLine": "/Dev1/PFI0", "445 Shutter-MaxVolts": "5.0000", "COM9-Handshaking": "Off", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "XLIGHT Spin motor-Label": "On (spinning)", "PRIME-PP 5 ENABLED": "No", "UUID": "3b083f03-b747-45a2-82f0-0cd3d20fde3f", "PRIME-TriggerMode": "Internal Trigger", "COM1-Handshaking": "Off", "PRIME-Timing-PostTriggerDelayNs": "49344000.0000", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "Focus-Position": "16732.9000", "528 Shutter-Block voltage": "false", "Channel": "Il-T", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "555 Shutter-Demo": "No", "405 Shutter-Demo": "No", "528 AO-Name": "AnalogIO", "520-State": "0", "Core-Galvo": "", "405 Shutter-TriggerSampleRate": "100000", "Focus-FocusStepSize_(nm)": "100", "PRIME-PixelType": "16bit", "COM4-DataBits": "8", "470 AO-IOChannel": "Dev1/ao2", "520 AO-MinVolts": "0.0000", "COM9-Parity": "None", "PRIME-CircularBufferEnabled": "ON", "PRIME-PP 6 Reset": "No", "640 AO-Description": "NI DAC", "Source": "PRIME", "445 AO-MinVolts": "0.0000", "PIEZO-Name": "Stage", "445 AO-Description": "NI DAC", "PRIME-CircularBufferFrameCount": "46", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "520 AO-Volts": "0.0000", "528 Shutter-TriggerSampleRate": "100000", "XPositionUm": 23269.100000000002, "OpticalPath-State": "4", "PRIME-PP 3 THRESHOLD": "80", "PRIME-PVCAM Version": "3.7.1", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Gain": "1-HDR", "640 Shutter-TriggerSequenceLength": "1000", "528 AO-SupportsTriggering": "0", "445 AO-TriggerInputLine": "/Dev1/PFI0", "OpticalPath-Name": "OpticalPath", "PRIME-PP 4 ENABLED": "Yes", "PRIME-PVCAM Adapter Version": "1.3.29", "COM3-Name": "COM3", "405 AO-MaxVolts": "5.0000", "520 Shutter-Block voltage": "false", "PRIME-Timing-ClearingTimeNs": "49344000.0000", "Core-Focus": "PIEZO", "528 AO-Block voltage": "false", "TE2000-Description": "Nikon TE2000 hub", "528 AO-MinVolts": "0.0000", "COM4-Parity": "None", "Summary": { "Slices": 11, "ChContrastMin": [ 0, 0, 0, 0, 0, 0 ], "PixelAspect": 1, "SlicesFirst": true, "Interval_ms": 0, "Time": "2019-12-17 03:59:33 -0500", "Prefix": "seq_7", "InitialPositionList": [ { "Label": "Pos0", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [149.91], "Focus": [16657.7], "XYStage": [ 71629.7, 19152.9 ] }, "GridColumnIndex": 0 }, { "Label": "Pos1", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [190.82], "Focus": [16485.4], "XYStage": [ 65702.3, 19396.600000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos2", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [194.69], "Focus": [16446.8], "XYStage": [ 59880.5, 19007.8 ] }, "GridColumnIndex": 0 }, { "Label": "Pos3", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [145.04], "Focus": [16446.6], "XYStage": [ 53616.9, 18818.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos4", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [169.57500000000002], "Focus": [16446.5], "XYStage": [ 47662.8, 18817.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos5", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [131.805], "Focus": [16446.4], "XYStage": [ 41918.8, 18298.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos6", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [113.145], "Focus": [16446.5], "XYStage": [ 36129.9, 18895.8 ] }, "GridColumnIndex": 0 }, { "Label": "Pos7", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [141.005], "Focus": [16446.6], "XYStage": [ 30299.100000000002, 18895.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos8", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [140.97], "Focus": [16446.5], "XYStage": [ 23952.100000000002, 19156 ] }, "GridColumnIndex": 0 }, { "Label": "Pos9", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [184.57500000000002], "Focus": [16677.9], "XYStage": [ 72866.8, -14809.300000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos10", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [167.95000000000002], "Focus": [16557.9], "XYStage": [ 66515.6, -14371.300000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos11", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [168], "Focus": [16618.9], "XYStage": [ 59724.3, -13964.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos12", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [203.205], "Focus": [16779.6], "XYStage": [ 53862.3, -13494.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos13", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [203.20000000000002], "Focus": [16749], "XYStage": [ 48108.3, -13805.400000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos14", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [203.19], "Focus": [16675.9], "XYStage": [ 41645, -13804.900000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos15", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [203.21], "Focus": [16675.7], "XYStage": [ 35352.6, -13805.6 ] }, "GridColumnIndex": 0 }, { "Label": "Pos16", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [203.225], "Focus": [16675.8], "XYStage": [ 29298, -13447.400000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos17", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [203.215], "Focus": [16675.8], "XYStage": [ 23269.100000000002, -13012.300000000001 ] }, "GridColumnIndex": 0 } ], "z-step_um": 10, "GridRow": 0, "BitDepth": 16, "Depth": 2, "Channels": 6, "Source": "Micro-Manager", "ChColors": [ -16711681, -256, -65281, -1, -1, -16776961 ], "MetadataVersion": 10, "KeepShutterOpenChannels": true, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536, 65536 ], "CustomIntervals_ms": [], "Height": 1200, "MicroManagerVersion": "1.4.23 20180219", "GridColumn": 0, "UUID": "3ad8801b-5699-40d6-813b-4ad840863e38", "PixelType": "GRAY16", "Width": 1200, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\Yan\\20191216-YC113-S11S12-miseq", "Frames": 1, "Comment": "", "UserName": "Nikon", "PixelSize_um": 1.1, "ROI": [ 0, 0, 1200, 1200 ], "Date": "2019-12-17", "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC", "P-DAPI-high" ], "KeepShutterOpenSlices": true, "IJType": 1, "ComputerName": "ZadorConfocal2", "Positions": 18, "PositionIndex": 17, "CameraTimeout": 20000, "TimeFirst": false }, "640 AO-Name": "AnalogIO", "Vincent-D3-Command": "Close" }, "FrameKey-0-1-4": { "COM4-StopBits": "1", "528 AO-MaxVolts": "5.0000", "XLIGHT Excitation Wheel-State": "1", "470 Shutter-Name": "AnalogIO", "Core-Shutter": "555", "555 AO-Demo": "No", "XLIGHT Dichroic-State": "2", "XYStage-StepSize-Y": "0.1000", "ZPositionUm": 193.215, "XYStage-StepSize-X": "0.1000", "PRIME-FirmwareVersion": "20.28", "PRIME-ClearCycles": "2", "520-Name": "DA Shutter", "405 Shutter-Name": "AnalogIO", "PIEZO-StepSize": "0.0050", "PRIME-ClearMode": "Pre-Exposure", "470 AO-MinVolts": "0.0000", "405 AO-SupportsTriggering": "1", "CameraChannelIndex": 0, "XYStage-StartSpeed": "500.0000", "COM9-StopBits": "2", "405 AO-Description": "NI DAC", "COM4-DelayBetweenCharsMs": "0.0000", "445 AO-Block voltage": "false", "520 AO-TriggerInputLine": "/Dev1/PFI0", "555 AO-Description": "NI DAC", "520 AO-TriggerSampleRate": "100000", "COM4-Verbose": "1", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM4-Name": "COM4", "405 Shutter-Volts": "0.0000", "Vincent-D3-Port": "COM1", "PRIME-PP 4 MIN ADU AFFECTED": "200", "470 Shutter-MinVolts": "0.0000", "PRIME-PP 3 ENABLED": "Yes", "470 AO-Description": "NI DAC", "PRIME-Description": "PVCAM API device adapter", "520 Shutter-MaxVolts": "5.0000", "520 Shutter-Volts": "0.0000", "528 AO-Sequenceable": "1", "PRIME-Binning": "1x1", "555 Shutter-MaxVolts": "5.0000", "528 Shutter-TriggerSequenceLength": "1000", "405 Shutter-Block voltage": "false", "XLIGHT Spin motor-State": "1", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "Vincent-D3-Time to open (ms)": "35.0000", "Vincent-D3-Time to close (ms)": "35.0000", "445 AO-TriggerSampleRate": "100000", "640 Shutter-IOChannel": "Dev1/ao14", "555 AO-Sequenceable": "1", "520 AO-Name": "AnalogIO", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "470 AO-Block voltage": "false", "COM1-Verbose": "1", "COM9-AnswerTimeout": "10000.0000", "528 Shutter-Name": "AnalogIO", "PRIME-Trigger Timeout (secs)": "10", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "NextFrame": 0, "470 AO-TriggerSequenceLength": "1000", "PRIME-TransposeXY": "0", "445 AO-TriggerSequenceLength": "1000", "COM1-Name": "COM1", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "COM9-DataBits": "8", "COM1-AnswerTimeout": "500.0000", "Camera": "", "TE2000-Version": "V2.50-V2.50-V2.10", "528 Shutter-Volts": "0.0000", "XLIGHT Hub-Name": "XLIGHT Hub", "PIEZO-Description": "Ludl stage driver adapter", "PositionName": "Pos17", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSampleRate": "100000", "PRIME-FanSpeedSetpoint": "High", "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "405 AO-TriggerSampleRate": "100000", "470 Shutter-Block voltage": "false", "470-State": "0", "COM3-Parity": "None", "405 AO-Volts": "0.0000", "PRIME-TransposeMirrorY": "0", "PRIME-ChipName": "GS144BSI", "470 AO-Volts": "0.0000", "PRIME-TransposeMirrorX": "0", "445 Shutter-SupportsTriggering": "0", "470 Shutter-SupportsTriggering": "0", "XLIGHT Hub-Description": "XLIGHT hub", "PRIME-PreampOffLimit": "10000", "470 AO-Sequenceable": "1", "528 Shutter-MaxVolts": "5.0000", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "Core-XYStage": "XYStage", "470 AO-TriggerSampleRate": "100000", "PRIME-Timing-PreTriggerDelayNs": "24672000.0000", "528-DA Device": "528 Shutter", "Focus-Description": "Nikon TE2000 focus adapter", "FilterBlock-State": "5", "520 AO-Demo": "No", "COM3-DelayBetweenCharsMs": "0.0000", "470 Shutter-Volts": "0.0000", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "PRIME-PP 2 THRESHOLD": "125", "PRIME-PP 4 THRESHOLD": "75", "XYStage-Acceleration": "4.0000", "470 Shutter-Demo": "No", "PRIME-Name": "PMPCIECam00", "640-DA Device": "640 Shutter", "405 AO-Block voltage": "false", "ElapsedTime-ms": 724874, "PRIME-Offset": "100", "445 AO-IOChannel": "Dev1/ao1", "640 AO-MaxVolts": "5.0000", "PositionIndex": 17, "PRIME-PP 1 ENABLED": "Yes", "COM1-BaudRate": "9600", "640-State": "0", "COM3-Verbose": "1", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "LudlController-TransmissionDelay": "0", "PRIME-FullWellCapacity": "62000", "PRIME-SMARTStreamingEnabled": "No", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "640 Shutter-Description": "NI DAC", "528-Description": "DA device that is used as a shutter", "COM4-Description": "Serial port driver (boost:asio)", "405 Shutter-Description": "NI DAC", "TE2000-Name": "TE2000", "528 Shutter-Description": "NI DAC", "COM9-Description": "Serial port driver (boost:asio)", "LudlController-Name": "LudlController", "445 Shutter-TriggerSequenceLength": "1000", "PRIME-SerialNumber": "A18B203008", "445 AO-Volts": "0.0000", "PRIME-X-dimension": "1200", "PRIME-ShutterCloseDelay": "0", "XLIGHT Disk slider-State": "2", "Position": "Default", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 AO-IOChannel": "Dev1/ao5", "520 AO-IOChannel": "Dev1/ao4", "528 AO-TriggerSequenceLength": "1000", "405 Shutter-MaxVolts": "5.0000", "470-DA Device": "470 Shutter", "PRIME-FTCapable": "No", "Core-ChannelGroup": "Channels", "555 AO-SupportsTriggering": "0", "PRIME-Y-dimension": "1200", "Frame": 0, "555 Shutter-Sequenceable": "1", "PRIME-Timing-ExposureTimeNs": "499998000.0000", "Core-ImageProcessor": "", "528-State": "0", "520 AO-Block voltage": "false", "XLIGHT Excitation Wheel-Label": "QUAD", "640 Shutter-Demo": "No", "COM3-StopBits": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "520 Shutter-IOChannel": "Dev1/ao11", "Width": 1200, "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-SupportsTriggering": "0", "640 AO-TriggerSampleRate": "100000", "520-Description": "DA device that is used as a shutter", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "405 Shutter-Sequenceable": "1", "405 Shutter-SupportsTriggering": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-TriggerSampleRate": "100000", "640 Shutter-MaxVolts": "5.0000", "555 AO-MaxVolts": "5.0000", "520 AO-TriggerSequenceLength": "1000", "COM3-BaudRate": "9600", "COM9-BaudRate": "9600", "Vincent-D3-Channel #": "Ch. #1", "PRIME-PMode": "Normal", "OpticalPath-Label": "Left(100)", "BitDepth": 16, "555 AO-TriggerSequenceLength": "1000", "445 Shutter-Description": "NI DAC", "405 AO-Sequenceable": "1", "PRIME-AcquisitionMethod": "Callbacks", "555 AO-Name": "AnalogIO", "PRIME-ShutterMode": "Pre-Exposure", "COM3-Handshaking": "Off", "445 AO-SupportsTriggering": "0", "ROI": "0-0-1200-1200", "COM9-Verbose": "1", "PRIME-Color": "OFF", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "PRIME-ShutterOpenDelay": "0", "COM1-Parity": "None", "Slice": 4, "SlicePosition": -10, "COM9-DelayBetweenCharsMs": "0.0000", "555-Name": "DA Shutter", "PIEZO-LudlSingleAxisName": "B", "XLIGHT Emission Wheel-Label": "585/11(T)", "640 Shutter-Name": "AnalogIO", "640 AO-Sequenceable": "1", "520 Shutter-Demo": "No", "528 Shutter-Demo": "No", "470-Name": "DA Shutter", "520-DA Device": "520 Shutter", "XYStage-Description": "Ludl XY stage driver adapter", "445 Shutter-MinVolts": "0.0000", "528 AO-TriggerSampleRate": "100000", "405 AO-MinVolts": "0.0000", "405 Shutter-TriggerSequenceLength": "1000", "Height": 1200, "555 AO-Block voltage": "false", "640 Shutter-Volts": "0.0000", "PixelType": "GRAY16", "445 Shutter-Block voltage": "false", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "520 Shutter-TriggerSequenceLength": "1000", "COM9-Name": "COM9", "Core-SLM": "", "555 AO-Volts": "4.5000", "555 Shutter-Block voltage": "false", "XYStage-Speed": "1800.0000", "520 AO-MaxVolts": "5.0000", "528 Shutter-SupportsTriggering": "0", "COM1-Description": "Serial port driver (boost:asio)", "PRIME-MetadataEnabled": "No", "520 Shutter-MinVolts": "0.0000", "470 Shutter-Sequenceable": "1", "PRIME-PreampDelay": "0", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-MinVolts": "0.0000", "ChannelIndex": 1, "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Core-AutoShutter": "1", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "Exposure-ms": 160, "PRIME-PP 2 ENABLED": "Yes", "640 Shutter-TriggerSampleRate": "100000", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "470 Shutter-IOChannel": "Dev1/ao10", "405 AO-Demo": "No", "PixelSizeUm": 1.1, "470 AO-Demo": "No", "470 Shutter-MaxVolts": "5.0000", "Vincent-D3-Name": "Vincent-D3", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FileName": "img_000000000_Il-T_004.tif", "LudlController-Reset": "Operate", "PRIME-Exposure": "160.00", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "445-Name": "DA Shutter", "555 Shutter-Name": "AnalogIO", "520 Shutter-TriggerSampleRate": "100000", "445 Shutter-IOChannel": "Dev1/ao9", "PRIME-TransposeCorrection": "0", "XYStage-StepSize": "0.1000", "445-Description": "DA device that is used as a shutter", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 AO-IOChannel": "Dev1/ao7", "520 Shutter-Name": "AnalogIO", "COM3-DataBits": "8", "640 AO-Volts": "0.0000", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "555 Shutter-IOChannel": "Dev1/ao13", "WaitInterval": null, "520 Shutter-Sequenceable": "1", "YPositionUm": -13012.300000000001, "445-State": "0", "445-DA Device": "445 Shutter", "PRIME-CircularBufferAutoSize": "ON", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "555 AO-TriggerSampleRate": "100000", "445 Shutter-Volts": "0.0000", "XLIGHT Hub-Port": "COM3", "Core-AutoFocus": "", "COM4-Handshaking": "Off", "528 Shutter-MinVolts": "0.0000", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "445 Shutter-Name": "AnalogIO", "528 Shutter-Sequenceable": "1", "XYStage-TransposeMirrorX": "0", "XYStage-TransposeMirrorY": "0", "PRIME-ReadoutRate": "100MHz 16bit", "XYStage-Name": "Stage", "640 AO-SupportsTriggering": "0", "640-Name": "DA Shutter", "640 Shutter-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "640 AO-Block voltage": "false", "445 Shutter-Sequenceable": "1", "470 AO-MaxVolts": "5.0000", "COM4-BaudRate": "9600", "470-Description": "DA device that is used as a shutter", "520 Shutter-Description": "NI DAC", "PRIME-CCDTemperature": "-14.9200", "Time": "2019-12-17 03:59:41 -0500", "640 AO-TriggerSequenceLength": "1000", "470 AO-Name": "AnalogIO", "XLIGHT Disk slider-Label": "Disk Pos 40um", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "Binning": "1x1", "TE2000-ModelType": "1", "COM3-AnswerTimeout": "500.0000", "555 Shutter-SupportsTriggering": "0", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "TE2000-Port": "COM4", "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Description": "NI DAC", "COM4-AnswerTimeout": "500.0000", "COM1-DelayBetweenCharsMs": "0.0000", "FilterBlock-Label": "Confocal", "445 AO-Name": "AnalogIO", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-Timing-ReadoutTimeNs": "24672000.0000", "528 AO-Demo": "No", "520 AO-Sequenceable": "1", "555-Description": "DA device that is used as a shutter", "405-DA Device": "405 Shutter", "405 AO-TriggerSequenceLength": "1000", "640 AO-MinVolts": "0.0000", "640 Shutter-Block voltage": "false", "FrameIndex": 0, "PRIME-Port": "CMOS", "555 AO-MinVolts": "0.0000", "COM3-Description": "Serial port driver (boost:asio)", "528 AO-Description": "NI DAC", "555 AO-IOChannel": "Dev1/ao6", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "555 AO-TriggerInputLine": "/Dev1/PFI0", "PRIME-ActualInterval-ms": "84.6200", "LudlController-Port": "COM9", "445 Shutter-Demo": "No", "PRIME-Trigger-Expose Out-Mux": "1", "SliceIndex": 4, "PRIME-CCDTemperatureSetPoint": "-15.0000", "405 Shutter-MinVolts": "0.0000", "PRIME-ExposeOutMode": "First Row", "AxisPositions": null, "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Volts": "5.0000", "528-Name": "DA Shutter", "555-State": "1", "XLIGHT Dichroic-Label": "QUAD", "445 AO-Sequenceable": "1", "405-Name": "DA Shutter", "405 AO-IOChannel": "Dev1/ao0", "Core-Camera": "PRIME", "Focus-Name": "Focus", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "405 Shutter-IOChannel": "Dev1/ao8", "640 AO-Demo": "No", "640 Shutter-SupportsTriggering": "0", "445 AO-Demo": "No", "555-DA Device": "555 Shutter", "470 Shutter-Description": "NI DAC", "528 AO-Volts": "0.0000", "COM1-DataBits": "8", "Vincent-D3-Address": "x", "520 AO-SupportsTriggering": "0", "405-Description": "DA device that is used as a shutter", "640 Shutter-MinVolts": "0.0000", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "405 AO-Name": "AnalogIO", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "640 AO-TriggerInputLine": "/Dev1/PFI0", "405 AO-TriggerInputLine": "/Dev1/PFI0", "640-Description": "DA device that is used as a shutter", "445 Shutter-TriggerSampleRate": "100000", "470 Shutter-TriggerSequenceLength": "1000", "COM1-StopBits": "1", "520 Shutter-SupportsTriggering": "0", "405-State": "0", "Core-Initialize": "1", "XLIGHT Emission Wheel-State": "3", "520 AO-Description": "NI DAC", "528 AO-TriggerInputLine": "/Dev1/PFI0", "445 Shutter-MaxVolts": "5.0000", "COM9-Handshaking": "Off", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "XLIGHT Spin motor-Label": "On (spinning)", "PRIME-PP 5 ENABLED": "No", "UUID": "3c1629b4-8287-4654-ad87-152dd7ea3940", "PRIME-TriggerMode": "Internal Trigger", "COM1-Handshaking": "Off", "PRIME-Timing-PostTriggerDelayNs": "49344000.0000", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "Focus-Position": "16732.9000", "528 Shutter-Block voltage": "false", "Channel": "Il-T", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "555 Shutter-Demo": "No", "405 Shutter-Demo": "No", "528 AO-Name": "AnalogIO", "520-State": "0", "Core-Galvo": "", "405 Shutter-TriggerSampleRate": "100000", "Focus-FocusStepSize_(nm)": "100", "PRIME-PixelType": "16bit", "COM4-DataBits": "8", "470 AO-IOChannel": "Dev1/ao2", "520 AO-MinVolts": "0.0000", "COM9-Parity": "None", "PRIME-CircularBufferEnabled": "ON", "PRIME-PP 6 Reset": "No", "640 AO-Description": "NI DAC", "Source": "PRIME", "445 AO-MinVolts": "0.0000", "PIEZO-Name": "Stage", "445 AO-Description": "NI DAC", "PRIME-CircularBufferFrameCount": "46", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "520 AO-Volts": "0.0000", "528 Shutter-TriggerSampleRate": "100000", "XPositionUm": 23269.100000000002, "OpticalPath-State": "4", "PRIME-PP 3 THRESHOLD": "80", "PRIME-PVCAM Version": "3.7.1", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Gain": "1-HDR", "640 Shutter-TriggerSequenceLength": "1000", "528 AO-SupportsTriggering": "0", "445 AO-TriggerInputLine": "/Dev1/PFI0", "OpticalPath-Name": "OpticalPath", "PRIME-PP 4 ENABLED": "Yes", "PRIME-PVCAM Adapter Version": "1.3.29", "COM3-Name": "COM3", "405 AO-MaxVolts": "5.0000", "520 Shutter-Block voltage": "false", "PRIME-Timing-ClearingTimeNs": "49344000.0000", "Core-Focus": "PIEZO", "528 AO-Block voltage": "false", "TE2000-Description": "Nikon TE2000 hub", "528 AO-MinVolts": "0.0000", "COM4-Parity": "None", "Summary": { "Slices": 11, "ChContrastMin": [ 0, 0, 0, 0, 0, 0 ], "PixelAspect": 1, "SlicesFirst": true, "Interval_ms": 0, "Time": "2019-12-17 03:59:33 -0500", "Prefix": "seq_7", "InitialPositionList": [ { "Label": "Pos0", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [149.91], "Focus": [16657.7], "XYStage": [ 71629.7, 19152.9 ] }, "GridColumnIndex": 0 }, { "Label": "Pos1", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [190.82], "Focus": [16485.4], "XYStage": [ 65702.3, 19396.600000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos2", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [194.69], "Focus": [16446.8], "XYStage": [ 59880.5, 19007.8 ] }, "GridColumnIndex": 0 }, { "Label": "Pos3", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [145.04], "Focus": [16446.6], "XYStage": [ 53616.9, 18818.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos4", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [169.57500000000002], "Focus": [16446.5], "XYStage": [ 47662.8, 18817.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos5", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [131.805], "Focus": [16446.4], "XYStage": [ 41918.8, 18298.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos6", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [113.145], "Focus": [16446.5], "XYStage": [ 36129.9, 18895.8 ] }, "GridColumnIndex": 0 }, { "Label": "Pos7", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [141.005], "Focus": [16446.6], "XYStage": [ 30299.100000000002, 18895.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos8", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [140.97], "Focus": [16446.5], "XYStage": [ 23952.100000000002, 19156 ] }, "GridColumnIndex": 0 }, { "Label": "Pos9", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [184.57500000000002], "Focus": [16677.9], "XYStage": [ 72866.8, -14809.300000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos10", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [167.95000000000002], "Focus": [16557.9], "XYStage": [ 66515.6, -14371.300000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos11", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [168], "Focus": [16618.9], "XYStage": [ 59724.3, -13964.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos12", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [203.205], "Focus": [16779.6], "XYStage": [ 53862.3, -13494.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos13", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [203.20000000000002], "Focus": [16749], "XYStage": [ 48108.3, -13805.400000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos14", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [203.19], "Focus": [16675.9], "XYStage": [ 41645, -13804.900000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos15", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [203.21], "Focus": [16675.7], "XYStage": [ 35352.6, -13805.6 ] }, "GridColumnIndex": 0 }, { "Label": "Pos16", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [203.225], "Focus": [16675.8], "XYStage": [ 29298, -13447.400000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos17", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [203.215], "Focus": [16675.8], "XYStage": [ 23269.100000000002, -13012.300000000001 ] }, "GridColumnIndex": 0 } ], "z-step_um": 10, "GridRow": 0, "BitDepth": 16, "Depth": 2, "Channels": 6, "Source": "Micro-Manager", "ChColors": [ -16711681, -256, -65281, -1, -1, -16776961 ], "MetadataVersion": 10, "KeepShutterOpenChannels": true, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536, 65536 ], "CustomIntervals_ms": [], "Height": 1200, "MicroManagerVersion": "1.4.23 20180219", "GridColumn": 0, "UUID": "3ad8801b-5699-40d6-813b-4ad840863e38", "PixelType": "GRAY16", "Width": 1200, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\Yan\\20191216-YC113-S11S12-miseq", "Frames": 1, "Comment": "", "UserName": "Nikon", "PixelSize_um": 1.1, "ROI": [ 0, 0, 1200, 1200 ], "Date": "2019-12-17", "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC", "P-DAPI-high" ], "KeepShutterOpenSlices": true, "IJType": 1, "ComputerName": "ZadorConfocal2", "Positions": 18, "PositionIndex": 17, "CameraTimeout": 20000, "TimeFirst": false }, "640 AO-Name": "AnalogIO", "Vincent-D3-Command": "Close" }, "FrameKey-0-1-5": { "COM4-StopBits": "1", "528 AO-MaxVolts": "5.0000", "XLIGHT Excitation Wheel-State": "1", "470 Shutter-Name": "AnalogIO", "Core-Shutter": "555", "555 AO-Demo": "No", "XLIGHT Dichroic-State": "2", "XYStage-StepSize-Y": "0.1000", "ZPositionUm": 203.215, "XYStage-StepSize-X": "0.1000", "PRIME-FirmwareVersion": "20.28", "PRIME-ClearCycles": "2", "520-Name": "DA Shutter", "405 Shutter-Name": "AnalogIO", "PIEZO-StepSize": "0.0050", "PRIME-ClearMode": "Pre-Exposure", "470 AO-MinVolts": "0.0000", "405 AO-SupportsTriggering": "1", "CameraChannelIndex": 0, "XYStage-StartSpeed": "500.0000", "COM9-StopBits": "2", "405 AO-Description": "NI DAC", "COM4-DelayBetweenCharsMs": "0.0000", "445 AO-Block voltage": "false", "520 AO-TriggerInputLine": "/Dev1/PFI0", "555 AO-Description": "NI DAC", "520 AO-TriggerSampleRate": "100000", "COM4-Verbose": "1", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM4-Name": "COM4", "405 Shutter-Volts": "0.0000", "Vincent-D3-Port": "COM1", "PRIME-PP 4 MIN ADU AFFECTED": "200", "470 Shutter-MinVolts": "0.0000", "PRIME-PP 3 ENABLED": "Yes", "470 AO-Description": "NI DAC", "PRIME-Description": "PVCAM API device adapter", "520 Shutter-MaxVolts": "5.0000", "520 Shutter-Volts": "0.0000", "528 AO-Sequenceable": "1", "PRIME-Binning": "1x1", "555 Shutter-MaxVolts": "5.0000", "528 Shutter-TriggerSequenceLength": "1000", "405 Shutter-Block voltage": "false", "XLIGHT Spin motor-State": "1", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "Vincent-D3-Time to open (ms)": "35.0000", "Vincent-D3-Time to close (ms)": "35.0000", "445 AO-TriggerSampleRate": "100000", "640 Shutter-IOChannel": "Dev1/ao14", "555 AO-Sequenceable": "1", "520 AO-Name": "AnalogIO", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "470 AO-Block voltage": "false", "COM1-Verbose": "1", "COM9-AnswerTimeout": "10000.0000", "528 Shutter-Name": "AnalogIO", "PRIME-Trigger Timeout (secs)": "10", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "NextFrame": 0, "470 AO-TriggerSequenceLength": "1000", "PRIME-TransposeXY": "0", "445 AO-TriggerSequenceLength": "1000", "COM1-Name": "COM1", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "COM9-DataBits": "8", "COM1-AnswerTimeout": "500.0000", "Camera": "", "TE2000-Version": "V2.50-V2.50-V2.10", "528 Shutter-Volts": "0.0000", "XLIGHT Hub-Name": "XLIGHT Hub", "PIEZO-Description": "Ludl stage driver adapter", "PositionName": "Pos17", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSampleRate": "100000", "PRIME-FanSpeedSetpoint": "High", "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "405 AO-TriggerSampleRate": "100000", "470 Shutter-Block voltage": "false", "470-State": "0", "COM3-Parity": "None", "405 AO-Volts": "0.0000", "PRIME-TransposeMirrorY": "0", "PRIME-ChipName": "GS144BSI", "470 AO-Volts": "0.0000", "PRIME-TransposeMirrorX": "0", "445 Shutter-SupportsTriggering": "0", "470 Shutter-SupportsTriggering": "0", "XLIGHT Hub-Description": "XLIGHT hub", "PRIME-PreampOffLimit": "10000", "470 AO-Sequenceable": "1", "528 Shutter-MaxVolts": "5.0000", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "Core-XYStage": "XYStage", "470 AO-TriggerSampleRate": "100000", "PRIME-Timing-PreTriggerDelayNs": "24672000.0000", "528-DA Device": "528 Shutter", "Focus-Description": "Nikon TE2000 focus adapter", "FilterBlock-State": "5", "520 AO-Demo": "No", "COM3-DelayBetweenCharsMs": "0.0000", "470 Shutter-Volts": "0.0000", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "PRIME-PP 2 THRESHOLD": "125", "PRIME-PP 4 THRESHOLD": "75", "XYStage-Acceleration": "4.0000", "470 Shutter-Demo": "No", "PRIME-Name": "PMPCIECam00", "640-DA Device": "640 Shutter", "405 AO-Block voltage": "false", "ElapsedTime-ms": 725282, "PRIME-Offset": "100", "445 AO-IOChannel": "Dev1/ao1", "640 AO-MaxVolts": "5.0000", "PositionIndex": 17, "PRIME-PP 1 ENABLED": "Yes", "COM1-BaudRate": "9600", "640-State": "0", "COM3-Verbose": "1", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "LudlController-TransmissionDelay": "0", "PRIME-FullWellCapacity": "62000", "PRIME-SMARTStreamingEnabled": "No", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "640 Shutter-Description": "NI DAC", "528-Description": "DA device that is used as a shutter", "COM4-Description": "Serial port driver (boost:asio)", "405 Shutter-Description": "NI DAC", "TE2000-Name": "TE2000", "528 Shutter-Description": "NI DAC", "COM9-Description": "Serial port driver (boost:asio)", "LudlController-Name": "LudlController", "445 Shutter-TriggerSequenceLength": "1000", "PRIME-SerialNumber": "A18B203008", "445 AO-Volts": "0.0000", "PRIME-X-dimension": "1200", "PRIME-ShutterCloseDelay": "0", "XLIGHT Disk slider-State": "2", "Position": "Default", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 AO-IOChannel": "Dev1/ao5", "520 AO-IOChannel": "Dev1/ao4", "528 AO-TriggerSequenceLength": "1000", "405 Shutter-MaxVolts": "5.0000", "470-DA Device": "470 Shutter", "PRIME-FTCapable": "No", "Core-ChannelGroup": "Channels", "555 AO-SupportsTriggering": "0", "PRIME-Y-dimension": "1200", "Frame": 0, "555 Shutter-Sequenceable": "1", "PRIME-Timing-ExposureTimeNs": "499998000.0000", "Core-ImageProcessor": "", "528-State": "0", "520 AO-Block voltage": "false", "XLIGHT Excitation Wheel-Label": "QUAD", "640 Shutter-Demo": "No", "COM3-StopBits": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "520 Shutter-IOChannel": "Dev1/ao11", "Width": 1200, "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-SupportsTriggering": "0", "640 AO-TriggerSampleRate": "100000", "520-Description": "DA device that is used as a shutter", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "405 Shutter-Sequenceable": "1", "405 Shutter-SupportsTriggering": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-TriggerSampleRate": "100000", "640 Shutter-MaxVolts": "5.0000", "555 AO-MaxVolts": "5.0000", "520 AO-TriggerSequenceLength": "1000", "COM3-BaudRate": "9600", "COM9-BaudRate": "9600", "Vincent-D3-Channel #": "Ch. #1", "PRIME-PMode": "Normal", "OpticalPath-Label": "Left(100)", "BitDepth": 16, "555 AO-TriggerSequenceLength": "1000", "445 Shutter-Description": "NI DAC", "405 AO-Sequenceable": "1", "PRIME-AcquisitionMethod": "Callbacks", "555 AO-Name": "AnalogIO", "PRIME-ShutterMode": "Pre-Exposure", "COM3-Handshaking": "Off", "445 AO-SupportsTriggering": "0", "ROI": "0-0-1200-1200", "COM9-Verbose": "1", "PRIME-Color": "OFF", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "PRIME-ShutterOpenDelay": "0", "COM1-Parity": "None", "Slice": 5, "SlicePosition": 0, "COM9-DelayBetweenCharsMs": "0.0000", "555-Name": "DA Shutter", "PIEZO-LudlSingleAxisName": "B", "XLIGHT Emission Wheel-Label": "585/11(T)", "640 Shutter-Name": "AnalogIO", "640 AO-Sequenceable": "1", "520 Shutter-Demo": "No", "528 Shutter-Demo": "No", "470-Name": "DA Shutter", "520-DA Device": "520 Shutter", "XYStage-Description": "Ludl XY stage driver adapter", "445 Shutter-MinVolts": "0.0000", "528 AO-TriggerSampleRate": "100000", "405 AO-MinVolts": "0.0000", "405 Shutter-TriggerSequenceLength": "1000", "Height": 1200, "555 AO-Block voltage": "false", "640 Shutter-Volts": "0.0000", "PixelType": "GRAY16", "445 Shutter-Block voltage": "false", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "520 Shutter-TriggerSequenceLength": "1000", "COM9-Name": "COM9", "Core-SLM": "", "555 AO-Volts": "4.5000", "555 Shutter-Block voltage": "false", "XYStage-Speed": "1800.0000", "520 AO-MaxVolts": "5.0000", "528 Shutter-SupportsTriggering": "0", "COM1-Description": "Serial port driver (boost:asio)", "PRIME-MetadataEnabled": "No", "520 Shutter-MinVolts": "0.0000", "470 Shutter-Sequenceable": "1", "PRIME-PreampDelay": "0", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-MinVolts": "0.0000", "ChannelIndex": 1, "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Core-AutoShutter": "1", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "Exposure-ms": 160, "PRIME-PP 2 ENABLED": "Yes", "640 Shutter-TriggerSampleRate": "100000", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "470 Shutter-IOChannel": "Dev1/ao10", "405 AO-Demo": "No", "PixelSizeUm": 1.1, "470 AO-Demo": "No", "470 Shutter-MaxVolts": "5.0000", "Vincent-D3-Name": "Vincent-D3", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FileName": "img_000000000_Il-T_005.tif", "LudlController-Reset": "Operate", "PRIME-Exposure": "160.00", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "445-Name": "DA Shutter", "555 Shutter-Name": "AnalogIO", "520 Shutter-TriggerSampleRate": "100000", "445 Shutter-IOChannel": "Dev1/ao9", "PRIME-TransposeCorrection": "0", "XYStage-StepSize": "0.1000", "445-Description": "DA device that is used as a shutter", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 AO-IOChannel": "Dev1/ao7", "520 Shutter-Name": "AnalogIO", "COM3-DataBits": "8", "640 AO-Volts": "0.0000", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "555 Shutter-IOChannel": "Dev1/ao13", "WaitInterval": null, "520 Shutter-Sequenceable": "1", "YPositionUm": -13012.300000000001, "445-State": "0", "445-DA Device": "445 Shutter", "PRIME-CircularBufferAutoSize": "ON", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "555 AO-TriggerSampleRate": "100000", "445 Shutter-Volts": "0.0000", "XLIGHT Hub-Port": "COM3", "Core-AutoFocus": "", "COM4-Handshaking": "Off", "528 Shutter-MinVolts": "0.0000", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "445 Shutter-Name": "AnalogIO", "528 Shutter-Sequenceable": "1", "XYStage-TransposeMirrorX": "0", "XYStage-TransposeMirrorY": "0", "PRIME-ReadoutRate": "100MHz 16bit", "XYStage-Name": "Stage", "640 AO-SupportsTriggering": "0", "640-Name": "DA Shutter", "640 Shutter-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "640 AO-Block voltage": "false", "445 Shutter-Sequenceable": "1", "470 AO-MaxVolts": "5.0000", "COM4-BaudRate": "9600", "470-Description": "DA device that is used as a shutter", "520 Shutter-Description": "NI DAC", "PRIME-CCDTemperature": "-14.9200", "Time": "2019-12-17 03:59:42 -0500", "640 AO-TriggerSequenceLength": "1000", "470 AO-Name": "AnalogIO", "XLIGHT Disk slider-Label": "Disk Pos 40um", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "Binning": "1x1", "TE2000-ModelType": "1", "COM3-AnswerTimeout": "500.0000", "555 Shutter-SupportsTriggering": "0", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "TE2000-Port": "COM4", "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Description": "NI DAC", "COM4-AnswerTimeout": "500.0000", "COM1-DelayBetweenCharsMs": "0.0000", "FilterBlock-Label": "Confocal", "445 AO-Name": "AnalogIO", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-Timing-ReadoutTimeNs": "24672000.0000", "528 AO-Demo": "No", "520 AO-Sequenceable": "1", "555-Description": "DA device that is used as a shutter", "405-DA Device": "405 Shutter", "405 AO-TriggerSequenceLength": "1000", "640 AO-MinVolts": "0.0000", "640 Shutter-Block voltage": "false", "FrameIndex": 0, "PRIME-Port": "CMOS", "555 AO-MinVolts": "0.0000", "COM3-Description": "Serial port driver (boost:asio)", "528 AO-Description": "NI DAC", "555 AO-IOChannel": "Dev1/ao6", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "555 AO-TriggerInputLine": "/Dev1/PFI0", "PRIME-ActualInterval-ms": "84.6200", "LudlController-Port": "COM9", "445 Shutter-Demo": "No", "PRIME-Trigger-Expose Out-Mux": "1", "SliceIndex": 5, "PRIME-CCDTemperatureSetPoint": "-15.0000", "405 Shutter-MinVolts": "0.0000", "PRIME-ExposeOutMode": "First Row", "AxisPositions": null, "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Volts": "5.0000", "528-Name": "DA Shutter", "555-State": "1", "XLIGHT Dichroic-Label": "QUAD", "445 AO-Sequenceable": "1", "405-Name": "DA Shutter", "405 AO-IOChannel": "Dev1/ao0", "Core-Camera": "PRIME", "Focus-Name": "Focus", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "405 Shutter-IOChannel": "Dev1/ao8", "640 AO-Demo": "No", "640 Shutter-SupportsTriggering": "0", "445 AO-Demo": "No", "555-DA Device": "555 Shutter", "470 Shutter-Description": "NI DAC", "528 AO-Volts": "0.0000", "COM1-DataBits": "8", "Vincent-D3-Address": "x", "520 AO-SupportsTriggering": "0", "405-Description": "DA device that is used as a shutter", "640 Shutter-MinVolts": "0.0000", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "405 AO-Name": "AnalogIO", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "640 AO-TriggerInputLine": "/Dev1/PFI0", "405 AO-TriggerInputLine": "/Dev1/PFI0", "640-Description": "DA device that is used as a shutter", "445 Shutter-TriggerSampleRate": "100000", "470 Shutter-TriggerSequenceLength": "1000", "COM1-StopBits": "1", "520 Shutter-SupportsTriggering": "0", "405-State": "0", "Core-Initialize": "1", "XLIGHT Emission Wheel-State": "3", "520 AO-Description": "NI DAC", "528 AO-TriggerInputLine": "/Dev1/PFI0", "445 Shutter-MaxVolts": "5.0000", "COM9-Handshaking": "Off", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "XLIGHT Spin motor-Label": "On (spinning)", "PRIME-PP 5 ENABLED": "No", "UUID": "d4f61f23-f06f-4a15-a90e-f1dbc5d10df6", "PRIME-TriggerMode": "Internal Trigger", "COM1-Handshaking": "Off", "PRIME-Timing-PostTriggerDelayNs": "49344000.0000", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "Focus-Position": "16732.9000", "528 Shutter-Block voltage": "false", "Channel": "Il-T", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "555 Shutter-Demo": "No", "405 Shutter-Demo": "No", "528 AO-Name": "AnalogIO", "520-State": "0", "Core-Galvo": "", "405 Shutter-TriggerSampleRate": "100000", "Focus-FocusStepSize_(nm)": "100", "PRIME-PixelType": "16bit", "COM4-DataBits": "8", "470 AO-IOChannel": "Dev1/ao2", "520 AO-MinVolts": "0.0000", "COM9-Parity": "None", "PRIME-CircularBufferEnabled": "ON", "PRIME-PP 6 Reset": "No", "640 AO-Description": "NI DAC", "Source": "PRIME", "445 AO-MinVolts": "0.0000", "PIEZO-Name": "Stage", "445 AO-Description": "NI DAC", "PRIME-CircularBufferFrameCount": "46", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "520 AO-Volts": "0.0000", "528 Shutter-TriggerSampleRate": "100000", "XPositionUm": 23269.100000000002, "OpticalPath-State": "4", "PRIME-PP 3 THRESHOLD": "80", "PRIME-PVCAM Version": "3.7.1", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Gain": "1-HDR", "640 Shutter-TriggerSequenceLength": "1000", "528 AO-SupportsTriggering": "0", "445 AO-TriggerInputLine": "/Dev1/PFI0", "OpticalPath-Name": "OpticalPath", "PRIME-PP 4 ENABLED": "Yes", "PRIME-PVCAM Adapter Version": "1.3.29", "COM3-Name": "COM3", "405 AO-MaxVolts": "5.0000", "520 Shutter-Block voltage": "false", "PRIME-Timing-ClearingTimeNs": "49344000.0000", "Core-Focus": "PIEZO", "528 AO-Block voltage": "false", "TE2000-Description": "Nikon TE2000 hub", "528 AO-MinVolts": "0.0000", "COM4-Parity": "None", "Summary": { "Slices": 11, "ChContrastMin": [ 0, 0, 0, 0, 0, 0 ], "PixelAspect": 1, "SlicesFirst": true, "Interval_ms": 0, "Time": "2019-12-17 03:59:33 -0500", "Prefix": "seq_7", "InitialPositionList": [ { "Label": "Pos0", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [149.91], "Focus": [16657.7], "XYStage": [ 71629.7, 19152.9 ] }, "GridColumnIndex": 0 }, { "Label": "Pos1", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [190.82], "Focus": [16485.4], "XYStage": [ 65702.3, 19396.600000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos2", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [194.69], "Focus": [16446.8], "XYStage": [ 59880.5, 19007.8 ] }, "GridColumnIndex": 0 }, { "Label": "Pos3", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [145.04], "Focus": [16446.6], "XYStage": [ 53616.9, 18818.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos4", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [169.57500000000002], "Focus": [16446.5], "XYStage": [ 47662.8, 18817.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos5", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [131.805], "Focus": [16446.4], "XYStage": [ 41918.8, 18298.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos6", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [113.145], "Focus": [16446.5], "XYStage": [ 36129.9, 18895.8 ] }, "GridColumnIndex": 0 }, { "Label": "Pos7", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [141.005], "Focus": [16446.6], "XYStage": [ 30299.100000000002, 18895.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos8", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [140.97], "Focus": [16446.5], "XYStage": [ 23952.100000000002, 19156 ] }, "GridColumnIndex": 0 }, { "Label": "Pos9", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [184.57500000000002], "Focus": [16677.9], "XYStage": [ 72866.8, -14809.300000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos10", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [167.95000000000002], "Focus": [16557.9], "XYStage": [ 66515.6, -14371.300000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos11", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [168], "Focus": [16618.9], "XYStage": [ 59724.3, -13964.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos12", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [203.205], "Focus": [16779.6], "XYStage": [ 53862.3, -13494.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos13", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [203.20000000000002], "Focus": [16749], "XYStage": [ 48108.3, -13805.400000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos14", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [203.19], "Focus": [16675.9], "XYStage": [ 41645, -13804.900000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos15", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [203.21], "Focus": [16675.7], "XYStage": [ 35352.6, -13805.6 ] }, "GridColumnIndex": 0 }, { "Label": "Pos16", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [203.225], "Focus": [16675.8], "XYStage": [ 29298, -13447.400000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos17", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [203.215], "Focus": [16675.8], "XYStage": [ 23269.100000000002, -13012.300000000001 ] }, "GridColumnIndex": 0 } ], "z-step_um": 10, "GridRow": 0, "BitDepth": 16, "Depth": 2, "Channels": 6, "Source": "Micro-Manager", "ChColors": [ -16711681, -256, -65281, -1, -1, -16776961 ], "MetadataVersion": 10, "KeepShutterOpenChannels": true, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536, 65536 ], "CustomIntervals_ms": [], "Height": 1200, "MicroManagerVersion": "1.4.23 20180219", "GridColumn": 0, "UUID": "3ad8801b-5699-40d6-813b-4ad840863e38", "PixelType": "GRAY16", "Width": 1200, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\Yan\\20191216-YC113-S11S12-miseq", "Frames": 1, "Comment": "", "UserName": "Nikon", "PixelSize_um": 1.1, "ROI": [ 0, 0, 1200, 1200 ], "Date": "2019-12-17", "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC", "P-DAPI-high" ], "KeepShutterOpenSlices": true, "IJType": 1, "ComputerName": "ZadorConfocal2", "Positions": 18, "PositionIndex": 17, "CameraTimeout": 20000, "TimeFirst": false }, "640 AO-Name": "AnalogIO", "Vincent-D3-Command": "Close" }, "FrameKey-0-1-6": { "COM4-StopBits": "1", "528 AO-MaxVolts": "5.0000", "XLIGHT Excitation Wheel-State": "1", "470 Shutter-Name": "AnalogIO", "Core-Shutter": "555", "555 AO-Demo": "No", "XLIGHT Dichroic-State": "2", "XYStage-StepSize-Y": "0.1000", "ZPositionUm": 213.215, "XYStage-StepSize-X": "0.1000", "PRIME-FirmwareVersion": "20.28", "PRIME-ClearCycles": "2", "520-Name": "DA Shutter", "405 Shutter-Name": "AnalogIO", "PIEZO-StepSize": "0.0050", "PRIME-ClearMode": "Pre-Exposure", "470 AO-MinVolts": "0.0000", "405 AO-SupportsTriggering": "1", "CameraChannelIndex": 0, "XYStage-StartSpeed": "500.0000", "COM9-StopBits": "2", "405 AO-Description": "NI DAC", "COM4-DelayBetweenCharsMs": "0.0000", "445 AO-Block voltage": "false", "520 AO-TriggerInputLine": "/Dev1/PFI0", "555 AO-Description": "NI DAC", "520 AO-TriggerSampleRate": "100000", "COM4-Verbose": "1", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM4-Name": "COM4", "405 Shutter-Volts": "0.0000", "Vincent-D3-Port": "COM1", "PRIME-PP 4 MIN ADU AFFECTED": "200", "470 Shutter-MinVolts": "0.0000", "PRIME-PP 3 ENABLED": "Yes", "470 AO-Description": "NI DAC", "PRIME-Description": "PVCAM API device adapter", "520 Shutter-MaxVolts": "5.0000", "520 Shutter-Volts": "0.0000", "528 AO-Sequenceable": "1", "PRIME-Binning": "1x1", "555 Shutter-MaxVolts": "5.0000", "528 Shutter-TriggerSequenceLength": "1000", "405 Shutter-Block voltage": "false", "XLIGHT Spin motor-State": "1", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "Vincent-D3-Time to open (ms)": "35.0000", "Vincent-D3-Time to close (ms)": "35.0000", "445 AO-TriggerSampleRate": "100000", "640 Shutter-IOChannel": "Dev1/ao14", "555 AO-Sequenceable": "1", "520 AO-Name": "AnalogIO", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "470 AO-Block voltage": "false", "COM1-Verbose": "1", "COM9-AnswerTimeout": "10000.0000", "528 Shutter-Name": "AnalogIO", "PRIME-Trigger Timeout (secs)": "10", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "NextFrame": 0, "470 AO-TriggerSequenceLength": "1000", "PRIME-TransposeXY": "0", "445 AO-TriggerSequenceLength": "1000", "COM1-Name": "COM1", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "COM9-DataBits": "8", "COM1-AnswerTimeout": "500.0000", "Camera": "", "TE2000-Version": "V2.50-V2.50-V2.10", "528 Shutter-Volts": "0.0000", "XLIGHT Hub-Name": "XLIGHT Hub", "PIEZO-Description": "Ludl stage driver adapter", "PositionName": "Pos17", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSampleRate": "100000", "PRIME-FanSpeedSetpoint": "High", "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "405 AO-TriggerSampleRate": "100000", "470 Shutter-Block voltage": "false", "470-State": "0", "COM3-Parity": "None", "405 AO-Volts": "0.0000", "PRIME-TransposeMirrorY": "0", "PRIME-ChipName": "GS144BSI", "470 AO-Volts": "0.0000", "PRIME-TransposeMirrorX": "0", "445 Shutter-SupportsTriggering": "0", "470 Shutter-SupportsTriggering": "0", "XLIGHT Hub-Description": "XLIGHT hub", "PRIME-PreampOffLimit": "10000", "470 AO-Sequenceable": "1", "528 Shutter-MaxVolts": "5.0000", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "Core-XYStage": "XYStage", "470 AO-TriggerSampleRate": "100000", "PRIME-Timing-PreTriggerDelayNs": "24672000.0000", "528-DA Device": "528 Shutter", "Focus-Description": "Nikon TE2000 focus adapter", "FilterBlock-State": "5", "520 AO-Demo": "No", "COM3-DelayBetweenCharsMs": "0.0000", "470 Shutter-Volts": "0.0000", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "PRIME-PP 2 THRESHOLD": "125", "PRIME-PP 4 THRESHOLD": "75", "XYStage-Acceleration": "4.0000", "470 Shutter-Demo": "No", "PRIME-Name": "PMPCIECam00", "640-DA Device": "640 Shutter", "405 AO-Block voltage": "false", "ElapsedTime-ms": 725677, "PRIME-Offset": "100", "445 AO-IOChannel": "Dev1/ao1", "640 AO-MaxVolts": "5.0000", "PositionIndex": 17, "PRIME-PP 1 ENABLED": "Yes", "COM1-BaudRate": "9600", "640-State": "0", "COM3-Verbose": "1", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "LudlController-TransmissionDelay": "0", "PRIME-FullWellCapacity": "62000", "PRIME-SMARTStreamingEnabled": "No", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "640 Shutter-Description": "NI DAC", "528-Description": "DA device that is used as a shutter", "COM4-Description": "Serial port driver (boost:asio)", "405 Shutter-Description": "NI DAC", "TE2000-Name": "TE2000", "528 Shutter-Description": "NI DAC", "COM9-Description": "Serial port driver (boost:asio)", "LudlController-Name": "LudlController", "445 Shutter-TriggerSequenceLength": "1000", "PRIME-SerialNumber": "A18B203008", "445 AO-Volts": "0.0000", "PRIME-X-dimension": "1200", "PRIME-ShutterCloseDelay": "0", "XLIGHT Disk slider-State": "2", "Position": "Default", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 AO-IOChannel": "Dev1/ao5", "520 AO-IOChannel": "Dev1/ao4", "528 AO-TriggerSequenceLength": "1000", "405 Shutter-MaxVolts": "5.0000", "470-DA Device": "470 Shutter", "PRIME-FTCapable": "No", "Core-ChannelGroup": "Channels", "555 AO-SupportsTriggering": "0", "PRIME-Y-dimension": "1200", "Frame": 0, "555 Shutter-Sequenceable": "1", "PRIME-Timing-ExposureTimeNs": "499998000.0000", "Core-ImageProcessor": "", "528-State": "0", "520 AO-Block voltage": "false", "XLIGHT Excitation Wheel-Label": "QUAD", "640 Shutter-Demo": "No", "COM3-StopBits": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "520 Shutter-IOChannel": "Dev1/ao11", "Width": 1200, "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-SupportsTriggering": "0", "640 AO-TriggerSampleRate": "100000", "520-Description": "DA device that is used as a shutter", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "405 Shutter-Sequenceable": "1", "405 Shutter-SupportsTriggering": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-TriggerSampleRate": "100000", "640 Shutter-MaxVolts": "5.0000", "555 AO-MaxVolts": "5.0000", "520 AO-TriggerSequenceLength": "1000", "COM3-BaudRate": "9600", "COM9-BaudRate": "9600", "Vincent-D3-Channel #": "Ch. #1", "PRIME-PMode": "Normal", "OpticalPath-Label": "Left(100)", "BitDepth": 16, "555 AO-TriggerSequenceLength": "1000", "445 Shutter-Description": "NI DAC", "405 AO-Sequenceable": "1", "PRIME-AcquisitionMethod": "Callbacks", "555 AO-Name": "AnalogIO", "PRIME-ShutterMode": "Pre-Exposure", "COM3-Handshaking": "Off", "445 AO-SupportsTriggering": "0", "ROI": "0-0-1200-1200", "COM9-Verbose": "1", "PRIME-Color": "OFF", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "PRIME-ShutterOpenDelay": "0", "COM1-Parity": "None", "Slice": 6, "SlicePosition": 10, "COM9-DelayBetweenCharsMs": "0.0000", "555-Name": "DA Shutter", "PIEZO-LudlSingleAxisName": "B", "XLIGHT Emission Wheel-Label": "585/11(T)", "640 Shutter-Name": "AnalogIO", "640 AO-Sequenceable": "1", "520 Shutter-Demo": "No", "528 Shutter-Demo": "No", "470-Name": "DA Shutter", "520-DA Device": "520 Shutter", "XYStage-Description": "Ludl XY stage driver adapter", "445 Shutter-MinVolts": "0.0000", "528 AO-TriggerSampleRate": "100000", "405 AO-MinVolts": "0.0000", "405 Shutter-TriggerSequenceLength": "1000", "Height": 1200, "555 AO-Block voltage": "false", "640 Shutter-Volts": "0.0000", "PixelType": "GRAY16", "445 Shutter-Block voltage": "false", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "520 Shutter-TriggerSequenceLength": "1000", "COM9-Name": "COM9", "Core-SLM": "", "555 AO-Volts": "4.5000", "555 Shutter-Block voltage": "false", "XYStage-Speed": "1800.0000", "520 AO-MaxVolts": "5.0000", "528 Shutter-SupportsTriggering": "0", "COM1-Description": "Serial port driver (boost:asio)", "PRIME-MetadataEnabled": "No", "520 Shutter-MinVolts": "0.0000", "470 Shutter-Sequenceable": "1", "PRIME-PreampDelay": "0", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-MinVolts": "0.0000", "ChannelIndex": 1, "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Core-AutoShutter": "1", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "Exposure-ms": 160, "PRIME-PP 2 ENABLED": "Yes", "640 Shutter-TriggerSampleRate": "100000", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "470 Shutter-IOChannel": "Dev1/ao10", "405 AO-Demo": "No", "PixelSizeUm": 1.1, "470 AO-Demo": "No", "470 Shutter-MaxVolts": "5.0000", "Vincent-D3-Name": "Vincent-D3", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FileName": "img_000000000_Il-T_006.tif", "LudlController-Reset": "Operate", "PRIME-Exposure": "160.00", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "445-Name": "DA Shutter", "555 Shutter-Name": "AnalogIO", "520 Shutter-TriggerSampleRate": "100000", "445 Shutter-IOChannel": "Dev1/ao9", "PRIME-TransposeCorrection": "0", "XYStage-StepSize": "0.1000", "445-Description": "DA device that is used as a shutter", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 AO-IOChannel": "Dev1/ao7", "520 Shutter-Name": "AnalogIO", "COM3-DataBits": "8", "640 AO-Volts": "0.0000", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "555 Shutter-IOChannel": "Dev1/ao13", "WaitInterval": null, "520 Shutter-Sequenceable": "1", "YPositionUm": -13012.300000000001, "445-State": "0", "445-DA Device": "445 Shutter", "PRIME-CircularBufferAutoSize": "ON", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "555 AO-TriggerSampleRate": "100000", "445 Shutter-Volts": "0.0000", "XLIGHT Hub-Port": "COM3", "Core-AutoFocus": "", "COM4-Handshaking": "Off", "528 Shutter-MinVolts": "0.0000", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "445 Shutter-Name": "AnalogIO", "528 Shutter-Sequenceable": "1", "XYStage-TransposeMirrorX": "0", "XYStage-TransposeMirrorY": "0", "PRIME-ReadoutRate": "100MHz 16bit", "XYStage-Name": "Stage", "640 AO-SupportsTriggering": "0", "640-Name": "DA Shutter", "640 Shutter-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "640 AO-Block voltage": "false", "445 Shutter-Sequenceable": "1", "470 AO-MaxVolts": "5.0000", "COM4-BaudRate": "9600", "470-Description": "DA device that is used as a shutter", "520 Shutter-Description": "NI DAC", "PRIME-CCDTemperature": "-14.9200", "Time": "2019-12-17 03:59:42 -0500", "640 AO-TriggerSequenceLength": "1000", "470 AO-Name": "AnalogIO", "XLIGHT Disk slider-Label": "Disk Pos 40um", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "Binning": "1x1", "TE2000-ModelType": "1", "COM3-AnswerTimeout": "500.0000", "555 Shutter-SupportsTriggering": "0", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "TE2000-Port": "COM4", "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Description": "NI DAC", "COM4-AnswerTimeout": "500.0000", "COM1-DelayBetweenCharsMs": "0.0000", "FilterBlock-Label": "Confocal", "445 AO-Name": "AnalogIO", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-Timing-ReadoutTimeNs": "24672000.0000", "528 AO-Demo": "No", "520 AO-Sequenceable": "1", "555-Description": "DA device that is used as a shutter", "405-DA Device": "405 Shutter", "405 AO-TriggerSequenceLength": "1000", "640 AO-MinVolts": "0.0000", "640 Shutter-Block voltage": "false", "FrameIndex": 0, "PRIME-Port": "CMOS", "555 AO-MinVolts": "0.0000", "COM3-Description": "Serial port driver (boost:asio)", "528 AO-Description": "NI DAC", "555 AO-IOChannel": "Dev1/ao6", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "555 AO-TriggerInputLine": "/Dev1/PFI0", "PRIME-ActualInterval-ms": "84.6200", "LudlController-Port": "COM9", "445 Shutter-Demo": "No", "PRIME-Trigger-Expose Out-Mux": "1", "SliceIndex": 6, "PRIME-CCDTemperatureSetPoint": "-15.0000", "405 Shutter-MinVolts": "0.0000", "PRIME-ExposeOutMode": "First Row", "AxisPositions": null, "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Volts": "5.0000", "528-Name": "DA Shutter", "555-State": "1", "XLIGHT Dichroic-Label": "QUAD", "445 AO-Sequenceable": "1", "405-Name": "DA Shutter", "405 AO-IOChannel": "Dev1/ao0", "Core-Camera": "PRIME", "Focus-Name": "Focus", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "405 Shutter-IOChannel": "Dev1/ao8", "640 AO-Demo": "No", "640 Shutter-SupportsTriggering": "0", "445 AO-Demo": "No", "555-DA Device": "555 Shutter", "470 Shutter-Description": "NI DAC", "528 AO-Volts": "0.0000", "COM1-DataBits": "8", "Vincent-D3-Address": "x", "520 AO-SupportsTriggering": "0", "405-Description": "DA device that is used as a shutter", "640 Shutter-MinVolts": "0.0000", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "405 AO-Name": "AnalogIO", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "640 AO-TriggerInputLine": "/Dev1/PFI0", "405 AO-TriggerInputLine": "/Dev1/PFI0", "640-Description": "DA device that is used as a shutter", "445 Shutter-TriggerSampleRate": "100000", "470 Shutter-TriggerSequenceLength": "1000", "COM1-StopBits": "1", "520 Shutter-SupportsTriggering": "0", "405-State": "0", "Core-Initialize": "1", "XLIGHT Emission Wheel-State": "3", "520 AO-Description": "NI DAC", "528 AO-TriggerInputLine": "/Dev1/PFI0", "445 Shutter-MaxVolts": "5.0000", "COM9-Handshaking": "Off", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "XLIGHT Spin motor-Label": "On (spinning)", "PRIME-PP 5 ENABLED": "No", "UUID": "d86f729a-cc0d-4ce5-ab6e-139c43265d36", "PRIME-TriggerMode": "Internal Trigger", "COM1-Handshaking": "Off", "PRIME-Timing-PostTriggerDelayNs": "49344000.0000", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "Focus-Position": "16732.9000", "528 Shutter-Block voltage": "false", "Channel": "Il-T", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "555 Shutter-Demo": "No", "405 Shutter-Demo": "No", "528 AO-Name": "AnalogIO", "520-State": "0", "Core-Galvo": "", "405 Shutter-TriggerSampleRate": "100000", "Focus-FocusStepSize_(nm)": "100", "PRIME-PixelType": "16bit", "COM4-DataBits": "8", "470 AO-IOChannel": "Dev1/ao2", "520 AO-MinVolts": "0.0000", "COM9-Parity": "None", "PRIME-CircularBufferEnabled": "ON", "PRIME-PP 6 Reset": "No", "640 AO-Description": "NI DAC", "Source": "PRIME", "445 AO-MinVolts": "0.0000", "PIEZO-Name": "Stage", "445 AO-Description": "NI DAC", "PRIME-CircularBufferFrameCount": "46", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "520 AO-Volts": "0.0000", "528 Shutter-TriggerSampleRate": "100000", "XPositionUm": 23269.100000000002, "OpticalPath-State": "4", "PRIME-PP 3 THRESHOLD": "80", "PRIME-PVCAM Version": "3.7.1", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Gain": "1-HDR", "640 Shutter-TriggerSequenceLength": "1000", "528 AO-SupportsTriggering": "0", "445 AO-TriggerInputLine": "/Dev1/PFI0", "OpticalPath-Name": "OpticalPath", "PRIME-PP 4 ENABLED": "Yes", "PRIME-PVCAM Adapter Version": "1.3.29", "COM3-Name": "COM3", "405 AO-MaxVolts": "5.0000", "520 Shutter-Block voltage": "false", "PRIME-Timing-ClearingTimeNs": "49344000.0000", "Core-Focus": "PIEZO", "528 AO-Block voltage": "false", "TE2000-Description": "Nikon TE2000 hub", "528 AO-MinVolts": "0.0000", "COM4-Parity": "None", "Summary": { "Slices": 11, "ChContrastMin": [ 0, 0, 0, 0, 0, 0 ], "PixelAspect": 1, "SlicesFirst": true, "Interval_ms": 0, "Time": "2019-12-17 03:59:33 -0500", "Prefix": "seq_7", "InitialPositionList": [ { "Label": "Pos0", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [149.91], "Focus": [16657.7], "XYStage": [ 71629.7, 19152.9 ] }, "GridColumnIndex": 0 }, { "Label": "Pos1", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [190.82], "Focus": [16485.4], "XYStage": [ 65702.3, 19396.600000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos2", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [194.69], "Focus": [16446.8], "XYStage": [ 59880.5, 19007.8 ] }, "GridColumnIndex": 0 }, { "Label": "Pos3", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [145.04], "Focus": [16446.6], "XYStage": [ 53616.9, 18818.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos4", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [169.57500000000002], "Focus": [16446.5], "XYStage": [ 47662.8, 18817.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos5", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [131.805], "Focus": [16446.4], "XYStage": [ 41918.8, 18298.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos6", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [113.145], "Focus": [16446.5], "XYStage": [ 36129.9, 18895.8 ] }, "GridColumnIndex": 0 }, { "Label": "Pos7", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [141.005], "Focus": [16446.6], "XYStage": [ 30299.100000000002, 18895.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos8", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [140.97], "Focus": [16446.5], "XYStage": [ 23952.100000000002, 19156 ] }, "GridColumnIndex": 0 }, { "Label": "Pos9", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [184.57500000000002], "Focus": [16677.9], "XYStage": [ 72866.8, -14809.300000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos10", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [167.95000000000002], "Focus": [16557.9], "XYStage": [ 66515.6, -14371.300000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos11", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [168], "Focus": [16618.9], "XYStage": [ 59724.3, -13964.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos12", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [203.205], "Focus": [16779.6], "XYStage": [ 53862.3, -13494.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos13", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [203.20000000000002], "Focus": [16749], "XYStage": [ 48108.3, -13805.400000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos14", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [203.19], "Focus": [16675.9], "XYStage": [ 41645, -13804.900000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos15", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [203.21], "Focus": [16675.7], "XYStage": [ 35352.6, -13805.6 ] }, "GridColumnIndex": 0 }, { "Label": "Pos16", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [203.225], "Focus": [16675.8], "XYStage": [ 29298, -13447.400000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos17", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [203.215], "Focus": [16675.8], "XYStage": [ 23269.100000000002, -13012.300000000001 ] }, "GridColumnIndex": 0 } ], "z-step_um": 10, "GridRow": 0, "BitDepth": 16, "Depth": 2, "Channels": 6, "Source": "Micro-Manager", "ChColors": [ -16711681, -256, -65281, -1, -1, -16776961 ], "MetadataVersion": 10, "KeepShutterOpenChannels": true, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536, 65536 ], "CustomIntervals_ms": [], "Height": 1200, "MicroManagerVersion": "1.4.23 20180219", "GridColumn": 0, "UUID": "3ad8801b-5699-40d6-813b-4ad840863e38", "PixelType": "GRAY16", "Width": 1200, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\Yan\\20191216-YC113-S11S12-miseq", "Frames": 1, "Comment": "", "UserName": "Nikon", "PixelSize_um": 1.1, "ROI": [ 0, 0, 1200, 1200 ], "Date": "2019-12-17", "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC", "P-DAPI-high" ], "KeepShutterOpenSlices": true, "IJType": 1, "ComputerName": "ZadorConfocal2", "Positions": 18, "PositionIndex": 17, "CameraTimeout": 20000, "TimeFirst": false }, "640 AO-Name": "AnalogIO", "Vincent-D3-Command": "Close" }, "FrameKey-0-1-7": { "COM4-StopBits": "1", "528 AO-MaxVolts": "5.0000", "XLIGHT Excitation Wheel-State": "1", "470 Shutter-Name": "AnalogIO", "Core-Shutter": "555", "555 AO-Demo": "No", "XLIGHT Dichroic-State": "2", "XYStage-StepSize-Y": "0.1000", "ZPositionUm": 223.215, "XYStage-StepSize-X": "0.1000", "PRIME-FirmwareVersion": "20.28", "PRIME-ClearCycles": "2", "520-Name": "DA Shutter", "405 Shutter-Name": "AnalogIO", "PIEZO-StepSize": "0.0050", "PRIME-ClearMode": "Pre-Exposure", "470 AO-MinVolts": "0.0000", "405 AO-SupportsTriggering": "1", "CameraChannelIndex": 0, "XYStage-StartSpeed": "500.0000", "COM9-StopBits": "2", "405 AO-Description": "NI DAC", "COM4-DelayBetweenCharsMs": "0.0000", "445 AO-Block voltage": "false", "520 AO-TriggerInputLine": "/Dev1/PFI0", "555 AO-Description": "NI DAC", "520 AO-TriggerSampleRate": "100000", "COM4-Verbose": "1", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM4-Name": "COM4", "405 Shutter-Volts": "0.0000", "Vincent-D3-Port": "COM1", "PRIME-PP 4 MIN ADU AFFECTED": "200", "470 Shutter-MinVolts": "0.0000", "PRIME-PP 3 ENABLED": "Yes", "470 AO-Description": "NI DAC", "PRIME-Description": "PVCAM API device adapter", "520 Shutter-MaxVolts": "5.0000", "520 Shutter-Volts": "0.0000", "528 AO-Sequenceable": "1", "PRIME-Binning": "1x1", "555 Shutter-MaxVolts": "5.0000", "528 Shutter-TriggerSequenceLength": "1000", "405 Shutter-Block voltage": "false", "XLIGHT Spin motor-State": "1", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "Vincent-D3-Time to open (ms)": "35.0000", "Vincent-D3-Time to close (ms)": "35.0000", "445 AO-TriggerSampleRate": "100000", "640 Shutter-IOChannel": "Dev1/ao14", "555 AO-Sequenceable": "1", "520 AO-Name": "AnalogIO", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "470 AO-Block voltage": "false", "COM1-Verbose": "1", "COM9-AnswerTimeout": "10000.0000", "528 Shutter-Name": "AnalogIO", "PRIME-Trigger Timeout (secs)": "10", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "NextFrame": 0, "470 AO-TriggerSequenceLength": "1000", "PRIME-TransposeXY": "0", "445 AO-TriggerSequenceLength": "1000", "COM1-Name": "COM1", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "COM9-DataBits": "8", "COM1-AnswerTimeout": "500.0000", "Camera": "", "TE2000-Version": "V2.50-V2.50-V2.10", "528 Shutter-Volts": "0.0000", "XLIGHT Hub-Name": "XLIGHT Hub", "PIEZO-Description": "Ludl stage driver adapter", "PositionName": "Pos17", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSampleRate": "100000", "PRIME-FanSpeedSetpoint": "High", "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "405 AO-TriggerSampleRate": "100000", "470 Shutter-Block voltage": "false", "470-State": "0", "COM3-Parity": "None", "405 AO-Volts": "0.0000", "PRIME-TransposeMirrorY": "0", "PRIME-ChipName": "GS144BSI", "470 AO-Volts": "0.0000", "PRIME-TransposeMirrorX": "0", "445 Shutter-SupportsTriggering": "0", "470 Shutter-SupportsTriggering": "0", "XLIGHT Hub-Description": "XLIGHT hub", "PRIME-PreampOffLimit": "10000", "470 AO-Sequenceable": "1", "528 Shutter-MaxVolts": "5.0000", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "Core-XYStage": "XYStage", "470 AO-TriggerSampleRate": "100000", "PRIME-Timing-PreTriggerDelayNs": "24672000.0000", "528-DA Device": "528 Shutter", "Focus-Description": "Nikon TE2000 focus adapter", "FilterBlock-State": "5", "520 AO-Demo": "No", "COM3-DelayBetweenCharsMs": "0.0000", "470 Shutter-Volts": "0.0000", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "PRIME-PP 2 THRESHOLD": "125", "PRIME-PP 4 THRESHOLD": "75", "XYStage-Acceleration": "4.0000", "470 Shutter-Demo": "No", "PRIME-Name": "PMPCIECam00", "640-DA Device": "640 Shutter", "405 AO-Block voltage": "false", "ElapsedTime-ms": 726087, "PRIME-Offset": "100", "445 AO-IOChannel": "Dev1/ao1", "640 AO-MaxVolts": "5.0000", "PositionIndex": 17, "PRIME-PP 1 ENABLED": "Yes", "COM1-BaudRate": "9600", "640-State": "0", "COM3-Verbose": "1", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "LudlController-TransmissionDelay": "0", "PRIME-FullWellCapacity": "62000", "PRIME-SMARTStreamingEnabled": "No", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "640 Shutter-Description": "NI DAC", "528-Description": "DA device that is used as a shutter", "COM4-Description": "Serial port driver (boost:asio)", "405 Shutter-Description": "NI DAC", "TE2000-Name": "TE2000", "528 Shutter-Description": "NI DAC", "COM9-Description": "Serial port driver (boost:asio)", "LudlController-Name": "LudlController", "445 Shutter-TriggerSequenceLength": "1000", "PRIME-SerialNumber": "A18B203008", "445 AO-Volts": "0.0000", "PRIME-X-dimension": "1200", "PRIME-ShutterCloseDelay": "0", "XLIGHT Disk slider-State": "2", "Position": "Default", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 AO-IOChannel": "Dev1/ao5", "520 AO-IOChannel": "Dev1/ao4", "528 AO-TriggerSequenceLength": "1000", "405 Shutter-MaxVolts": "5.0000", "470-DA Device": "470 Shutter", "PRIME-FTCapable": "No", "Core-ChannelGroup": "Channels", "555 AO-SupportsTriggering": "0", "PRIME-Y-dimension": "1200", "Frame": 0, "555 Shutter-Sequenceable": "1", "PRIME-Timing-ExposureTimeNs": "499998000.0000", "Core-ImageProcessor": "", "528-State": "0", "520 AO-Block voltage": "false", "XLIGHT Excitation Wheel-Label": "QUAD", "640 Shutter-Demo": "No", "COM3-StopBits": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "520 Shutter-IOChannel": "Dev1/ao11", "Width": 1200, "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-SupportsTriggering": "0", "640 AO-TriggerSampleRate": "100000", "520-Description": "DA device that is used as a shutter", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "405 Shutter-Sequenceable": "1", "405 Shutter-SupportsTriggering": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-TriggerSampleRate": "100000", "640 Shutter-MaxVolts": "5.0000", "555 AO-MaxVolts": "5.0000", "520 AO-TriggerSequenceLength": "1000", "COM3-BaudRate": "9600", "COM9-BaudRate": "9600", "Vincent-D3-Channel #": "Ch. #1", "PRIME-PMode": "Normal", "OpticalPath-Label": "Left(100)", "BitDepth": 16, "555 AO-TriggerSequenceLength": "1000", "445 Shutter-Description": "NI DAC", "405 AO-Sequenceable": "1", "PRIME-AcquisitionMethod": "Callbacks", "555 AO-Name": "AnalogIO", "PRIME-ShutterMode": "Pre-Exposure", "COM3-Handshaking": "Off", "445 AO-SupportsTriggering": "0", "ROI": "0-0-1200-1200", "COM9-Verbose": "1", "PRIME-Color": "OFF", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "PRIME-ShutterOpenDelay": "0", "COM1-Parity": "None", "Slice": 7, "SlicePosition": 20, "COM9-DelayBetweenCharsMs": "0.0000", "555-Name": "DA Shutter", "PIEZO-LudlSingleAxisName": "B", "XLIGHT Emission Wheel-Label": "585/11(T)", "640 Shutter-Name": "AnalogIO", "640 AO-Sequenceable": "1", "520 Shutter-Demo": "No", "528 Shutter-Demo": "No", "470-Name": "DA Shutter", "520-DA Device": "520 Shutter", "XYStage-Description": "Ludl XY stage driver adapter", "445 Shutter-MinVolts": "0.0000", "528 AO-TriggerSampleRate": "100000", "405 AO-MinVolts": "0.0000", "405 Shutter-TriggerSequenceLength": "1000", "Height": 1200, "555 AO-Block voltage": "false", "640 Shutter-Volts": "0.0000", "PixelType": "GRAY16", "445 Shutter-Block voltage": "false", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "520 Shutter-TriggerSequenceLength": "1000", "COM9-Name": "COM9", "Core-SLM": "", "555 AO-Volts": "4.5000", "555 Shutter-Block voltage": "false", "XYStage-Speed": "1800.0000", "520 AO-MaxVolts": "5.0000", "528 Shutter-SupportsTriggering": "0", "COM1-Description": "Serial port driver (boost:asio)", "PRIME-MetadataEnabled": "No", "520 Shutter-MinVolts": "0.0000", "470 Shutter-Sequenceable": "1", "PRIME-PreampDelay": "0", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-MinVolts": "0.0000", "ChannelIndex": 1, "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Core-AutoShutter": "1", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "Exposure-ms": 160, "PRIME-PP 2 ENABLED": "Yes", "640 Shutter-TriggerSampleRate": "100000", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "470 Shutter-IOChannel": "Dev1/ao10", "405 AO-Demo": "No", "PixelSizeUm": 1.1, "470 AO-Demo": "No", "470 Shutter-MaxVolts": "5.0000", "Vincent-D3-Name": "Vincent-D3", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FileName": "img_000000000_Il-T_007.tif", "LudlController-Reset": "Operate", "PRIME-Exposure": "160.00", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "445-Name": "DA Shutter", "555 Shutter-Name": "AnalogIO", "520 Shutter-TriggerSampleRate": "100000", "445 Shutter-IOChannel": "Dev1/ao9", "PRIME-TransposeCorrection": "0", "XYStage-StepSize": "0.1000", "445-Description": "DA device that is used as a shutter", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 AO-IOChannel": "Dev1/ao7", "520 Shutter-Name": "AnalogIO", "COM3-DataBits": "8", "640 AO-Volts": "0.0000", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "555 Shutter-IOChannel": "Dev1/ao13", "WaitInterval": null, "520 Shutter-Sequenceable": "1", "YPositionUm": -13012.300000000001, "445-State": "0", "445-DA Device": "445 Shutter", "PRIME-CircularBufferAutoSize": "ON", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "555 AO-TriggerSampleRate": "100000", "445 Shutter-Volts": "0.0000", "XLIGHT Hub-Port": "COM3", "Core-AutoFocus": "", "COM4-Handshaking": "Off", "528 Shutter-MinVolts": "0.0000", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "445 Shutter-Name": "AnalogIO", "528 Shutter-Sequenceable": "1", "XYStage-TransposeMirrorX": "0", "XYStage-TransposeMirrorY": "0", "PRIME-ReadoutRate": "100MHz 16bit", "XYStage-Name": "Stage", "640 AO-SupportsTriggering": "0", "640-Name": "DA Shutter", "640 Shutter-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "640 AO-Block voltage": "false", "445 Shutter-Sequenceable": "1", "470 AO-MaxVolts": "5.0000", "COM4-BaudRate": "9600", "470-Description": "DA device that is used as a shutter", "520 Shutter-Description": "NI DAC", "PRIME-CCDTemperature": "-14.9200", "Time": "2019-12-17 03:59:43 -0500", "640 AO-TriggerSequenceLength": "1000", "470 AO-Name": "AnalogIO", "XLIGHT Disk slider-Label": "Disk Pos 40um", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "Binning": "1x1", "TE2000-ModelType": "1", "COM3-AnswerTimeout": "500.0000", "555 Shutter-SupportsTriggering": "0", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "TE2000-Port": "COM4", "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Description": "NI DAC", "COM4-AnswerTimeout": "500.0000", "COM1-DelayBetweenCharsMs": "0.0000", "FilterBlock-Label": "Confocal", "445 AO-Name": "AnalogIO", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-Timing-ReadoutTimeNs": "24672000.0000", "528 AO-Demo": "No", "520 AO-Sequenceable": "1", "555-Description": "DA device that is used as a shutter", "405-DA Device": "405 Shutter", "405 AO-TriggerSequenceLength": "1000", "640 AO-MinVolts": "0.0000", "640 Shutter-Block voltage": "false", "FrameIndex": 0, "PRIME-Port": "CMOS", "555 AO-MinVolts": "0.0000", "COM3-Description": "Serial port driver (boost:asio)", "528 AO-Description": "NI DAC", "555 AO-IOChannel": "Dev1/ao6", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "555 AO-TriggerInputLine": "/Dev1/PFI0", "PRIME-ActualInterval-ms": "84.6200", "LudlController-Port": "COM9", "445 Shutter-Demo": "No", "PRIME-Trigger-Expose Out-Mux": "1", "SliceIndex": 7, "PRIME-CCDTemperatureSetPoint": "-15.0000", "405 Shutter-MinVolts": "0.0000", "PRIME-ExposeOutMode": "First Row", "AxisPositions": null, "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Volts": "5.0000", "528-Name": "DA Shutter", "555-State": "1", "XLIGHT Dichroic-Label": "QUAD", "445 AO-Sequenceable": "1", "405-Name": "DA Shutter", "405 AO-IOChannel": "Dev1/ao0", "Core-Camera": "PRIME", "Focus-Name": "Focus", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "405 Shutter-IOChannel": "Dev1/ao8", "640 AO-Demo": "No", "640 Shutter-SupportsTriggering": "0", "445 AO-Demo": "No", "555-DA Device": "555 Shutter", "470 Shutter-Description": "NI DAC", "528 AO-Volts": "0.0000", "COM1-DataBits": "8", "Vincent-D3-Address": "x", "520 AO-SupportsTriggering": "0", "405-Description": "DA device that is used as a shutter", "640 Shutter-MinVolts": "0.0000", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "405 AO-Name": "AnalogIO", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "640 AO-TriggerInputLine": "/Dev1/PFI0", "405 AO-TriggerInputLine": "/Dev1/PFI0", "640-Description": "DA device that is used as a shutter", "445 Shutter-TriggerSampleRate": "100000", "470 Shutter-TriggerSequenceLength": "1000", "COM1-StopBits": "1", "520 Shutter-SupportsTriggering": "0", "405-State": "0", "Core-Initialize": "1", "XLIGHT Emission Wheel-State": "3", "520 AO-Description": "NI DAC", "528 AO-TriggerInputLine": "/Dev1/PFI0", "445 Shutter-MaxVolts": "5.0000", "COM9-Handshaking": "Off", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "XLIGHT Spin motor-Label": "On (spinning)", "PRIME-PP 5 ENABLED": "No", "UUID": "2776ec8a-4b81-4e1a-88aa-520ea453dce4", "PRIME-TriggerMode": "Internal Trigger", "COM1-Handshaking": "Off", "PRIME-Timing-PostTriggerDelayNs": "49344000.0000", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "Focus-Position": "16732.9000", "528 Shutter-Block voltage": "false", "Channel": "Il-T", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "555 Shutter-Demo": "No", "405 Shutter-Demo": "No", "528 AO-Name": "AnalogIO", "520-State": "0", "Core-Galvo": "", "405 Shutter-TriggerSampleRate": "100000", "Focus-FocusStepSize_(nm)": "100", "PRIME-PixelType": "16bit", "COM4-DataBits": "8", "470 AO-IOChannel": "Dev1/ao2", "520 AO-MinVolts": "0.0000", "COM9-Parity": "None", "PRIME-CircularBufferEnabled": "ON", "PRIME-PP 6 Reset": "No", "640 AO-Description": "NI DAC", "Source": "PRIME", "445 AO-MinVolts": "0.0000", "PIEZO-Name": "Stage", "445 AO-Description": "NI DAC", "PRIME-CircularBufferFrameCount": "46", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "520 AO-Volts": "0.0000", "528 Shutter-TriggerSampleRate": "100000", "XPositionUm": 23269.100000000002, "OpticalPath-State": "4", "PRIME-PP 3 THRESHOLD": "80", "PRIME-PVCAM Version": "3.7.1", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Gain": "1-HDR", "640 Shutter-TriggerSequenceLength": "1000", "528 AO-SupportsTriggering": "0", "445 AO-TriggerInputLine": "/Dev1/PFI0", "OpticalPath-Name": "OpticalPath", "PRIME-PP 4 ENABLED": "Yes", "PRIME-PVCAM Adapter Version": "1.3.29", "COM3-Name": "COM3", "405 AO-MaxVolts": "5.0000", "520 Shutter-Block voltage": "false", "PRIME-Timing-ClearingTimeNs": "49344000.0000", "Core-Focus": "PIEZO", "528 AO-Block voltage": "false", "TE2000-Description": "Nikon TE2000 hub", "528 AO-MinVolts": "0.0000", "COM4-Parity": "None", "Summary": { "Slices": 11, "ChContrastMin": [ 0, 0, 0, 0, 0, 0 ], "PixelAspect": 1, "SlicesFirst": true, "Interval_ms": 0, "Time": "2019-12-17 03:59:33 -0500", "Prefix": "seq_7", "InitialPositionList": [ { "Label": "Pos0", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [149.91], "Focus": [16657.7], "XYStage": [ 71629.7, 19152.9 ] }, "GridColumnIndex": 0 }, { "Label": "Pos1", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [190.82], "Focus": [16485.4], "XYStage": [ 65702.3, 19396.600000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos2", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [194.69], "Focus": [16446.8], "XYStage": [ 59880.5, 19007.8 ] }, "GridColumnIndex": 0 }, { "Label": "Pos3", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [145.04], "Focus": [16446.6], "XYStage": [ 53616.9, 18818.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos4", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [169.57500000000002], "Focus": [16446.5], "XYStage": [ 47662.8, 18817.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos5", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [131.805], "Focus": [16446.4], "XYStage": [ 41918.8, 18298.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos6", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [113.145], "Focus": [16446.5], "XYStage": [ 36129.9, 18895.8 ] }, "GridColumnIndex": 0 }, { "Label": "Pos7", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [141.005], "Focus": [16446.6], "XYStage": [ 30299.100000000002, 18895.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos8", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [140.97], "Focus": [16446.5], "XYStage": [ 23952.100000000002, 19156 ] }, "GridColumnIndex": 0 }, { "Label": "Pos9", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [184.57500000000002], "Focus": [16677.9], "XYStage": [ 72866.8, -14809.300000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos10", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [167.95000000000002], "Focus": [16557.9], "XYStage": [ 66515.6, -14371.300000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos11", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [168], "Focus": [16618.9], "XYStage": [ 59724.3, -13964.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos12", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [203.205], "Focus": [16779.6], "XYStage": [ 53862.3, -13494.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos13", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [203.20000000000002], "Focus": [16749], "XYStage": [ 48108.3, -13805.400000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos14", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [203.19], "Focus": [16675.9], "XYStage": [ 41645, -13804.900000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos15", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [203.21], "Focus": [16675.7], "XYStage": [ 35352.6, -13805.6 ] }, "GridColumnIndex": 0 }, { "Label": "Pos16", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [203.225], "Focus": [16675.8], "XYStage": [ 29298, -13447.400000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos17", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [203.215], "Focus": [16675.8], "XYStage": [ 23269.100000000002, -13012.300000000001 ] }, "GridColumnIndex": 0 } ], "z-step_um": 10, "GridRow": 0, "BitDepth": 16, "Depth": 2, "Channels": 6, "Source": "Micro-Manager", "ChColors": [ -16711681, -256, -65281, -1, -1, -16776961 ], "MetadataVersion": 10, "KeepShutterOpenChannels": true, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536, 65536 ], "CustomIntervals_ms": [], "Height": 1200, "MicroManagerVersion": "1.4.23 20180219", "GridColumn": 0, "UUID": "3ad8801b-5699-40d6-813b-4ad840863e38", "PixelType": "GRAY16", "Width": 1200, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\Yan\\20191216-YC113-S11S12-miseq", "Frames": 1, "Comment": "", "UserName": "Nikon", "PixelSize_um": 1.1, "ROI": [ 0, 0, 1200, 1200 ], "Date": "2019-12-17", "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC", "P-DAPI-high" ], "KeepShutterOpenSlices": true, "IJType": 1, "ComputerName": "ZadorConfocal2", "Positions": 18, "PositionIndex": 17, "CameraTimeout": 20000, "TimeFirst": false }, "640 AO-Name": "AnalogIO", "Vincent-D3-Command": "Close" }, "FrameKey-0-1-8": { "COM4-StopBits": "1", "528 AO-MaxVolts": "5.0000", "XLIGHT Excitation Wheel-State": "1", "470 Shutter-Name": "AnalogIO", "Core-Shutter": "555", "555 AO-Demo": "No", "XLIGHT Dichroic-State": "2", "XYStage-StepSize-Y": "0.1000", "ZPositionUm": 233.215, "XYStage-StepSize-X": "0.1000", "PRIME-FirmwareVersion": "20.28", "PRIME-ClearCycles": "2", "520-Name": "DA Shutter", "405 Shutter-Name": "AnalogIO", "PIEZO-StepSize": "0.0050", "PRIME-ClearMode": "Pre-Exposure", "470 AO-MinVolts": "0.0000", "405 AO-SupportsTriggering": "1", "CameraChannelIndex": 0, "XYStage-StartSpeed": "500.0000", "COM9-StopBits": "2", "405 AO-Description": "NI DAC", "COM4-DelayBetweenCharsMs": "0.0000", "445 AO-Block voltage": "false", "520 AO-TriggerInputLine": "/Dev1/PFI0", "555 AO-Description": "NI DAC", "520 AO-TriggerSampleRate": "100000", "COM4-Verbose": "1", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM4-Name": "COM4", "405 Shutter-Volts": "0.0000", "Vincent-D3-Port": "COM1", "PRIME-PP 4 MIN ADU AFFECTED": "200", "470 Shutter-MinVolts": "0.0000", "PRIME-PP 3 ENABLED": "Yes", "470 AO-Description": "NI DAC", "PRIME-Description": "PVCAM API device adapter", "520 Shutter-MaxVolts": "5.0000", "520 Shutter-Volts": "0.0000", "528 AO-Sequenceable": "1", "PRIME-Binning": "1x1", "555 Shutter-MaxVolts": "5.0000", "528 Shutter-TriggerSequenceLength": "1000", "405 Shutter-Block voltage": "false", "XLIGHT Spin motor-State": "1", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "Vincent-D3-Time to open (ms)": "35.0000", "Vincent-D3-Time to close (ms)": "35.0000", "445 AO-TriggerSampleRate": "100000", "640 Shutter-IOChannel": "Dev1/ao14", "555 AO-Sequenceable": "1", "520 AO-Name": "AnalogIO", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "470 AO-Block voltage": "false", "COM1-Verbose": "1", "COM9-AnswerTimeout": "10000.0000", "528 Shutter-Name": "AnalogIO", "PRIME-Trigger Timeout (secs)": "10", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "NextFrame": 0, "470 AO-TriggerSequenceLength": "1000", "PRIME-TransposeXY": "0", "445 AO-TriggerSequenceLength": "1000", "COM1-Name": "COM1", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "COM9-DataBits": "8", "COM1-AnswerTimeout": "500.0000", "Camera": "", "TE2000-Version": "V2.50-V2.50-V2.10", "528 Shutter-Volts": "0.0000", "XLIGHT Hub-Name": "XLIGHT Hub", "PIEZO-Description": "Ludl stage driver adapter", "PositionName": "Pos17", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSampleRate": "100000", "PRIME-FanSpeedSetpoint": "High", "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "405 AO-TriggerSampleRate": "100000", "470 Shutter-Block voltage": "false", "470-State": "0", "COM3-Parity": "None", "405 AO-Volts": "0.0000", "PRIME-TransposeMirrorY": "0", "PRIME-ChipName": "GS144BSI", "470 AO-Volts": "0.0000", "PRIME-TransposeMirrorX": "0", "445 Shutter-SupportsTriggering": "0", "470 Shutter-SupportsTriggering": "0", "XLIGHT Hub-Description": "XLIGHT hub", "PRIME-PreampOffLimit": "10000", "470 AO-Sequenceable": "1", "528 Shutter-MaxVolts": "5.0000", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "Core-XYStage": "XYStage", "470 AO-TriggerSampleRate": "100000", "PRIME-Timing-PreTriggerDelayNs": "24672000.0000", "528-DA Device": "528 Shutter", "Focus-Description": "Nikon TE2000 focus adapter", "FilterBlock-State": "5", "520 AO-Demo": "No", "COM3-DelayBetweenCharsMs": "0.0000", "470 Shutter-Volts": "0.0000", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "PRIME-PP 2 THRESHOLD": "125", "PRIME-PP 4 THRESHOLD": "75", "XYStage-Acceleration": "4.0000", "470 Shutter-Demo": "No", "PRIME-Name": "PMPCIECam00", "640-DA Device": "640 Shutter", "405 AO-Block voltage": "false", "ElapsedTime-ms": 726488, "PRIME-Offset": "100", "445 AO-IOChannel": "Dev1/ao1", "640 AO-MaxVolts": "5.0000", "PositionIndex": 17, "PRIME-PP 1 ENABLED": "Yes", "COM1-BaudRate": "9600", "640-State": "0", "COM3-Verbose": "1", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "LudlController-TransmissionDelay": "0", "PRIME-FullWellCapacity": "62000", "PRIME-SMARTStreamingEnabled": "No", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "640 Shutter-Description": "NI DAC", "528-Description": "DA device that is used as a shutter", "COM4-Description": "Serial port driver (boost:asio)", "405 Shutter-Description": "NI DAC", "TE2000-Name": "TE2000", "528 Shutter-Description": "NI DAC", "COM9-Description": "Serial port driver (boost:asio)", "LudlController-Name": "LudlController", "445 Shutter-TriggerSequenceLength": "1000", "PRIME-SerialNumber": "A18B203008", "445 AO-Volts": "0.0000", "PRIME-X-dimension": "1200", "PRIME-ShutterCloseDelay": "0", "XLIGHT Disk slider-State": "2", "Position": "Default", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 AO-IOChannel": "Dev1/ao5", "520 AO-IOChannel": "Dev1/ao4", "528 AO-TriggerSequenceLength": "1000", "405 Shutter-MaxVolts": "5.0000", "470-DA Device": "470 Shutter", "PRIME-FTCapable": "No", "Core-ChannelGroup": "Channels", "555 AO-SupportsTriggering": "0", "PRIME-Y-dimension": "1200", "Frame": 0, "555 Shutter-Sequenceable": "1", "PRIME-Timing-ExposureTimeNs": "499998000.0000", "Core-ImageProcessor": "", "528-State": "0", "520 AO-Block voltage": "false", "XLIGHT Excitation Wheel-Label": "QUAD", "640 Shutter-Demo": "No", "COM3-StopBits": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "520 Shutter-IOChannel": "Dev1/ao11", "Width": 1200, "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-SupportsTriggering": "0", "640 AO-TriggerSampleRate": "100000", "520-Description": "DA device that is used as a shutter", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "405 Shutter-Sequenceable": "1", "405 Shutter-SupportsTriggering": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-TriggerSampleRate": "100000", "640 Shutter-MaxVolts": "5.0000", "555 AO-MaxVolts": "5.0000", "520 AO-TriggerSequenceLength": "1000", "COM3-BaudRate": "9600", "COM9-BaudRate": "9600", "Vincent-D3-Channel #": "Ch. #1", "PRIME-PMode": "Normal", "OpticalPath-Label": "Left(100)", "BitDepth": 16, "555 AO-TriggerSequenceLength": "1000", "445 Shutter-Description": "NI DAC", "405 AO-Sequenceable": "1", "PRIME-AcquisitionMethod": "Callbacks", "555 AO-Name": "AnalogIO", "PRIME-ShutterMode": "Pre-Exposure", "COM3-Handshaking": "Off", "445 AO-SupportsTriggering": "0", "ROI": "0-0-1200-1200", "COM9-Verbose": "1", "PRIME-Color": "OFF", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "PRIME-ShutterOpenDelay": "0", "COM1-Parity": "None", "Slice": 8, "SlicePosition": 30, "COM9-DelayBetweenCharsMs": "0.0000", "555-Name": "DA Shutter", "PIEZO-LudlSingleAxisName": "B", "XLIGHT Emission Wheel-Label": "585/11(T)", "640 Shutter-Name": "AnalogIO", "640 AO-Sequenceable": "1", "520 Shutter-Demo": "No", "528 Shutter-Demo": "No", "470-Name": "DA Shutter", "520-DA Device": "520 Shutter", "XYStage-Description": "Ludl XY stage driver adapter", "445 Shutter-MinVolts": "0.0000", "528 AO-TriggerSampleRate": "100000", "405 AO-MinVolts": "0.0000", "405 Shutter-TriggerSequenceLength": "1000", "Height": 1200, "555 AO-Block voltage": "false", "640 Shutter-Volts": "0.0000", "PixelType": "GRAY16", "445 Shutter-Block voltage": "false", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "520 Shutter-TriggerSequenceLength": "1000", "COM9-Name": "COM9", "Core-SLM": "", "555 AO-Volts": "4.5000", "555 Shutter-Block voltage": "false", "XYStage-Speed": "1800.0000", "520 AO-MaxVolts": "5.0000", "528 Shutter-SupportsTriggering": "0", "COM1-Description": "Serial port driver (boost:asio)", "PRIME-MetadataEnabled": "No", "520 Shutter-MinVolts": "0.0000", "470 Shutter-Sequenceable": "1", "PRIME-PreampDelay": "0", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-MinVolts": "0.0000", "ChannelIndex": 1, "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Core-AutoShutter": "1", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "Exposure-ms": 160, "PRIME-PP 2 ENABLED": "Yes", "640 Shutter-TriggerSampleRate": "100000", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "470 Shutter-IOChannel": "Dev1/ao10", "405 AO-Demo": "No", "PixelSizeUm": 1.1, "470 AO-Demo": "No", "470 Shutter-MaxVolts": "5.0000", "Vincent-D3-Name": "Vincent-D3", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FileName": "img_000000000_Il-T_008.tif", "LudlController-Reset": "Operate", "PRIME-Exposure": "160.00", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "445-Name": "DA Shutter", "555 Shutter-Name": "AnalogIO", "520 Shutter-TriggerSampleRate": "100000", "445 Shutter-IOChannel": "Dev1/ao9", "PRIME-TransposeCorrection": "0", "XYStage-StepSize": "0.1000", "445-Description": "DA device that is used as a shutter", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 AO-IOChannel": "Dev1/ao7", "520 Shutter-Name": "AnalogIO", "COM3-DataBits": "8", "640 AO-Volts": "0.0000", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "555 Shutter-IOChannel": "Dev1/ao13", "WaitInterval": null, "520 Shutter-Sequenceable": "1", "YPositionUm": -13012.300000000001, "445-State": "0", "445-DA Device": "445 Shutter", "PRIME-CircularBufferAutoSize": "ON", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "555 AO-TriggerSampleRate": "100000", "445 Shutter-Volts": "0.0000", "XLIGHT Hub-Port": "COM3", "Core-AutoFocus": "", "COM4-Handshaking": "Off", "528 Shutter-MinVolts": "0.0000", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "445 Shutter-Name": "AnalogIO", "528 Shutter-Sequenceable": "1", "XYStage-TransposeMirrorX": "0", "XYStage-TransposeMirrorY": "0", "PRIME-ReadoutRate": "100MHz 16bit", "XYStage-Name": "Stage", "640 AO-SupportsTriggering": "0", "640-Name": "DA Shutter", "640 Shutter-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "640 AO-Block voltage": "false", "445 Shutter-Sequenceable": "1", "470 AO-MaxVolts": "5.0000", "COM4-BaudRate": "9600", "470-Description": "DA device that is used as a shutter", "520 Shutter-Description": "NI DAC", "PRIME-CCDTemperature": "-14.9200", "Time": "2019-12-17 03:59:43 -0500", "640 AO-TriggerSequenceLength": "1000", "470 AO-Name": "AnalogIO", "XLIGHT Disk slider-Label": "Disk Pos 40um", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "Binning": "1x1", "TE2000-ModelType": "1", "COM3-AnswerTimeout": "500.0000", "555 Shutter-SupportsTriggering": "0", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "TE2000-Port": "COM4", "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Description": "NI DAC", "COM4-AnswerTimeout": "500.0000", "COM1-DelayBetweenCharsMs": "0.0000", "FilterBlock-Label": "Confocal", "445 AO-Name": "AnalogIO", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-Timing-ReadoutTimeNs": "24672000.0000", "528 AO-Demo": "No", "520 AO-Sequenceable": "1", "555-Description": "DA device that is used as a shutter", "405-DA Device": "405 Shutter", "405 AO-TriggerSequenceLength": "1000", "640 AO-MinVolts": "0.0000", "640 Shutter-Block voltage": "false", "FrameIndex": 0, "PRIME-Port": "CMOS", "555 AO-MinVolts": "0.0000", "COM3-Description": "Serial port driver (boost:asio)", "528 AO-Description": "NI DAC", "555 AO-IOChannel": "Dev1/ao6", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "555 AO-TriggerInputLine": "/Dev1/PFI0", "PRIME-ActualInterval-ms": "84.6200", "LudlController-Port": "COM9", "445 Shutter-Demo": "No", "PRIME-Trigger-Expose Out-Mux": "1", "SliceIndex": 8, "PRIME-CCDTemperatureSetPoint": "-15.0000", "405 Shutter-MinVolts": "0.0000", "PRIME-ExposeOutMode": "First Row", "AxisPositions": null, "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Volts": "5.0000", "528-Name": "DA Shutter", "555-State": "1", "XLIGHT Dichroic-Label": "QUAD", "445 AO-Sequenceable": "1", "405-Name": "DA Shutter", "405 AO-IOChannel": "Dev1/ao0", "Core-Camera": "PRIME", "Focus-Name": "Focus", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "405 Shutter-IOChannel": "Dev1/ao8", "640 AO-Demo": "No", "640 Shutter-SupportsTriggering": "0", "445 AO-Demo": "No", "555-DA Device": "555 Shutter", "470 Shutter-Description": "NI DAC", "528 AO-Volts": "0.0000", "COM1-DataBits": "8", "Vincent-D3-Address": "x", "520 AO-SupportsTriggering": "0", "405-Description": "DA device that is used as a shutter", "640 Shutter-MinVolts": "0.0000", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "405 AO-Name": "AnalogIO", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "640 AO-TriggerInputLine": "/Dev1/PFI0", "405 AO-TriggerInputLine": "/Dev1/PFI0", "640-Description": "DA device that is used as a shutter", "445 Shutter-TriggerSampleRate": "100000", "470 Shutter-TriggerSequenceLength": "1000", "COM1-StopBits": "1", "520 Shutter-SupportsTriggering": "0", "405-State": "0", "Core-Initialize": "1", "XLIGHT Emission Wheel-State": "3", "520 AO-Description": "NI DAC", "528 AO-TriggerInputLine": "/Dev1/PFI0", "445 Shutter-MaxVolts": "5.0000", "COM9-Handshaking": "Off", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "XLIGHT Spin motor-Label": "On (spinning)", "PRIME-PP 5 ENABLED": "No", "UUID": "9f637ef0-03e9-4cf8-9b70-ba5c6d6fc2a1", "PRIME-TriggerMode": "Internal Trigger", "COM1-Handshaking": "Off", "PRIME-Timing-PostTriggerDelayNs": "49344000.0000", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "Focus-Position": "16732.9000", "528 Shutter-Block voltage": "false", "Channel": "Il-T", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "555 Shutter-Demo": "No", "405 Shutter-Demo": "No", "528 AO-Name": "AnalogIO", "520-State": "0", "Core-Galvo": "", "405 Shutter-TriggerSampleRate": "100000", "Focus-FocusStepSize_(nm)": "100", "PRIME-PixelType": "16bit", "COM4-DataBits": "8", "470 AO-IOChannel": "Dev1/ao2", "520 AO-MinVolts": "0.0000", "COM9-Parity": "None", "PRIME-CircularBufferEnabled": "ON", "PRIME-PP 6 Reset": "No", "640 AO-Description": "NI DAC", "Source": "PRIME", "445 AO-MinVolts": "0.0000", "PIEZO-Name": "Stage", "445 AO-Description": "NI DAC", "PRIME-CircularBufferFrameCount": "46", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "520 AO-Volts": "0.0000", "528 Shutter-TriggerSampleRate": "100000", "XPositionUm": 23269.100000000002, "OpticalPath-State": "4", "PRIME-PP 3 THRESHOLD": "80", "PRIME-PVCAM Version": "3.7.1", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Gain": "1-HDR", "640 Shutter-TriggerSequenceLength": "1000", "528 AO-SupportsTriggering": "0", "445 AO-TriggerInputLine": "/Dev1/PFI0", "OpticalPath-Name": "OpticalPath", "PRIME-PP 4 ENABLED": "Yes", "PRIME-PVCAM Adapter Version": "1.3.29", "COM3-Name": "COM3", "405 AO-MaxVolts": "5.0000", "520 Shutter-Block voltage": "false", "PRIME-Timing-ClearingTimeNs": "49344000.0000", "Core-Focus": "PIEZO", "528 AO-Block voltage": "false", "TE2000-Description": "Nikon TE2000 hub", "528 AO-MinVolts": "0.0000", "COM4-Parity": "None", "Summary": { "Slices": 11, "ChContrastMin": [ 0, 0, 0, 0, 0, 0 ], "PixelAspect": 1, "SlicesFirst": true, "Interval_ms": 0, "Time": "2019-12-17 03:59:33 -0500", "Prefix": "seq_7", "InitialPositionList": [ { "Label": "Pos0", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [149.91], "Focus": [16657.7], "XYStage": [ 71629.7, 19152.9 ] }, "GridColumnIndex": 0 }, { "Label": "Pos1", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [190.82], "Focus": [16485.4], "XYStage": [ 65702.3, 19396.600000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos2", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [194.69], "Focus": [16446.8], "XYStage": [ 59880.5, 19007.8 ] }, "GridColumnIndex": 0 }, { "Label": "Pos3", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [145.04], "Focus": [16446.6], "XYStage": [ 53616.9, 18818.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos4", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [169.57500000000002], "Focus": [16446.5], "XYStage": [ 47662.8, 18817.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos5", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [131.805], "Focus": [16446.4], "XYStage": [ 41918.8, 18298.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos6", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [113.145], "Focus": [16446.5], "XYStage": [ 36129.9, 18895.8 ] }, "GridColumnIndex": 0 }, { "Label": "Pos7", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [141.005], "Focus": [16446.6], "XYStage": [ 30299.100000000002, 18895.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos8", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [140.97], "Focus": [16446.5], "XYStage": [ 23952.100000000002, 19156 ] }, "GridColumnIndex": 0 }, { "Label": "Pos9", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [184.57500000000002], "Focus": [16677.9], "XYStage": [ 72866.8, -14809.300000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos10", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [167.95000000000002], "Focus": [16557.9], "XYStage": [ 66515.6, -14371.300000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos11", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [168], "Focus": [16618.9], "XYStage": [ 59724.3, -13964.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos12", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [203.205], "Focus": [16779.6], "XYStage": [ 53862.3, -13494.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos13", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [203.20000000000002], "Focus": [16749], "XYStage": [ 48108.3, -13805.400000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos14", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [203.19], "Focus": [16675.9], "XYStage": [ 41645, -13804.900000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos15", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [203.21], "Focus": [16675.7], "XYStage": [ 35352.6, -13805.6 ] }, "GridColumnIndex": 0 }, { "Label": "Pos16", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [203.225], "Focus": [16675.8], "XYStage": [ 29298, -13447.400000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos17", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [203.215], "Focus": [16675.8], "XYStage": [ 23269.100000000002, -13012.300000000001 ] }, "GridColumnIndex": 0 } ], "z-step_um": 10, "GridRow": 0, "BitDepth": 16, "Depth": 2, "Channels": 6, "Source": "Micro-Manager", "ChColors": [ -16711681, -256, -65281, -1, -1, -16776961 ], "MetadataVersion": 10, "KeepShutterOpenChannels": true, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536, 65536 ], "CustomIntervals_ms": [], "Height": 1200, "MicroManagerVersion": "1.4.23 20180219", "GridColumn": 0, "UUID": "3ad8801b-5699-40d6-813b-4ad840863e38", "PixelType": "GRAY16", "Width": 1200, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\Yan\\20191216-YC113-S11S12-miseq", "Frames": 1, "Comment": "", "UserName": "Nikon", "PixelSize_um": 1.1, "ROI": [ 0, 0, 1200, 1200 ], "Date": "2019-12-17", "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC", "P-DAPI-high" ], "KeepShutterOpenSlices": true, "IJType": 1, "ComputerName": "ZadorConfocal2", "Positions": 18, "PositionIndex": 17, "CameraTimeout": 20000, "TimeFirst": false }, "640 AO-Name": "AnalogIO", "Vincent-D3-Command": "Close" }, "FrameKey-0-1-9": { "COM4-StopBits": "1", "528 AO-MaxVolts": "5.0000", "XLIGHT Excitation Wheel-State": "1", "470 Shutter-Name": "AnalogIO", "Core-Shutter": "555", "555 AO-Demo": "No", "XLIGHT Dichroic-State": "2", "XYStage-StepSize-Y": "0.1000", "ZPositionUm": 243.215, "XYStage-StepSize-X": "0.1000", "PRIME-FirmwareVersion": "20.28", "PRIME-ClearCycles": "2", "520-Name": "DA Shutter", "405 Shutter-Name": "AnalogIO", "PIEZO-StepSize": "0.0050", "PRIME-ClearMode": "Pre-Exposure", "470 AO-MinVolts": "0.0000", "405 AO-SupportsTriggering": "1", "CameraChannelIndex": 0, "XYStage-StartSpeed": "500.0000", "COM9-StopBits": "2", "405 AO-Description": "NI DAC", "COM4-DelayBetweenCharsMs": "0.0000", "445 AO-Block voltage": "false", "520 AO-TriggerInputLine": "/Dev1/PFI0", "555 AO-Description": "NI DAC", "520 AO-TriggerSampleRate": "100000", "COM4-Verbose": "1", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM4-Name": "COM4", "405 Shutter-Volts": "0.0000", "Vincent-D3-Port": "COM1", "PRIME-PP 4 MIN ADU AFFECTED": "200", "470 Shutter-MinVolts": "0.0000", "PRIME-PP 3 ENABLED": "Yes", "470 AO-Description": "NI DAC", "PRIME-Description": "PVCAM API device adapter", "520 Shutter-MaxVolts": "5.0000", "520 Shutter-Volts": "0.0000", "528 AO-Sequenceable": "1", "PRIME-Binning": "1x1", "555 Shutter-MaxVolts": "5.0000", "528 Shutter-TriggerSequenceLength": "1000", "405 Shutter-Block voltage": "false", "XLIGHT Spin motor-State": "1", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "Vincent-D3-Time to open (ms)": "35.0000", "Vincent-D3-Time to close (ms)": "35.0000", "445 AO-TriggerSampleRate": "100000", "640 Shutter-IOChannel": "Dev1/ao14", "555 AO-Sequenceable": "1", "520 AO-Name": "AnalogIO", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "470 AO-Block voltage": "false", "COM1-Verbose": "1", "COM9-AnswerTimeout": "10000.0000", "528 Shutter-Name": "AnalogIO", "PRIME-Trigger Timeout (secs)": "10", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "NextFrame": 0, "470 AO-TriggerSequenceLength": "1000", "PRIME-TransposeXY": "0", "445 AO-TriggerSequenceLength": "1000", "COM1-Name": "COM1", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "COM9-DataBits": "8", "COM1-AnswerTimeout": "500.0000", "Camera": "", "TE2000-Version": "V2.50-V2.50-V2.10", "528 Shutter-Volts": "0.0000", "XLIGHT Hub-Name": "XLIGHT Hub", "PIEZO-Description": "Ludl stage driver adapter", "PositionName": "Pos17", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSampleRate": "100000", "PRIME-FanSpeedSetpoint": "High", "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "405 AO-TriggerSampleRate": "100000", "470 Shutter-Block voltage": "false", "470-State": "0", "COM3-Parity": "None", "405 AO-Volts": "0.0000", "PRIME-TransposeMirrorY": "0", "PRIME-ChipName": "GS144BSI", "470 AO-Volts": "0.0000", "PRIME-TransposeMirrorX": "0", "445 Shutter-SupportsTriggering": "0", "470 Shutter-SupportsTriggering": "0", "XLIGHT Hub-Description": "XLIGHT hub", "PRIME-PreampOffLimit": "10000", "470 AO-Sequenceable": "1", "528 Shutter-MaxVolts": "5.0000", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "Core-XYStage": "XYStage", "470 AO-TriggerSampleRate": "100000", "PRIME-Timing-PreTriggerDelayNs": "24672000.0000", "528-DA Device": "528 Shutter", "Focus-Description": "Nikon TE2000 focus adapter", "FilterBlock-State": "5", "520 AO-Demo": "No", "COM3-DelayBetweenCharsMs": "0.0000", "470 Shutter-Volts": "0.0000", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "PRIME-PP 2 THRESHOLD": "125", "PRIME-PP 4 THRESHOLD": "75", "XYStage-Acceleration": "4.0000", "470 Shutter-Demo": "No", "PRIME-Name": "PMPCIECam00", "640-DA Device": "640 Shutter", "405 AO-Block voltage": "false", "ElapsedTime-ms": 726888, "PRIME-Offset": "100", "445 AO-IOChannel": "Dev1/ao1", "640 AO-MaxVolts": "5.0000", "PositionIndex": 17, "PRIME-PP 1 ENABLED": "Yes", "COM1-BaudRate": "9600", "640-State": "0", "COM3-Verbose": "1", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "LudlController-TransmissionDelay": "0", "PRIME-FullWellCapacity": "62000", "PRIME-SMARTStreamingEnabled": "No", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "640 Shutter-Description": "NI DAC", "528-Description": "DA device that is used as a shutter", "COM4-Description": "Serial port driver (boost:asio)", "405 Shutter-Description": "NI DAC", "TE2000-Name": "TE2000", "528 Shutter-Description": "NI DAC", "COM9-Description": "Serial port driver (boost:asio)", "LudlController-Name": "LudlController", "445 Shutter-TriggerSequenceLength": "1000", "PRIME-SerialNumber": "A18B203008", "445 AO-Volts": "0.0000", "PRIME-X-dimension": "1200", "PRIME-ShutterCloseDelay": "0", "XLIGHT Disk slider-State": "2", "Position": "Default", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 AO-IOChannel": "Dev1/ao5", "520 AO-IOChannel": "Dev1/ao4", "528 AO-TriggerSequenceLength": "1000", "405 Shutter-MaxVolts": "5.0000", "470-DA Device": "470 Shutter", "PRIME-FTCapable": "No", "Core-ChannelGroup": "Channels", "555 AO-SupportsTriggering": "0", "PRIME-Y-dimension": "1200", "Frame": 0, "555 Shutter-Sequenceable": "1", "PRIME-Timing-ExposureTimeNs": "499998000.0000", "Core-ImageProcessor": "", "528-State": "0", "520 AO-Block voltage": "false", "XLIGHT Excitation Wheel-Label": "QUAD", "640 Shutter-Demo": "No", "COM3-StopBits": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "520 Shutter-IOChannel": "Dev1/ao11", "Width": 1200, "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-SupportsTriggering": "0", "640 AO-TriggerSampleRate": "100000", "520-Description": "DA device that is used as a shutter", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "405 Shutter-Sequenceable": "1", "405 Shutter-SupportsTriggering": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-TriggerSampleRate": "100000", "640 Shutter-MaxVolts": "5.0000", "555 AO-MaxVolts": "5.0000", "520 AO-TriggerSequenceLength": "1000", "COM3-BaudRate": "9600", "COM9-BaudRate": "9600", "Vincent-D3-Channel #": "Ch. #1", "PRIME-PMode": "Normal", "OpticalPath-Label": "Left(100)", "BitDepth": 16, "555 AO-TriggerSequenceLength": "1000", "445 Shutter-Description": "NI DAC", "405 AO-Sequenceable": "1", "PRIME-AcquisitionMethod": "Callbacks", "555 AO-Name": "AnalogIO", "PRIME-ShutterMode": "Pre-Exposure", "COM3-Handshaking": "Off", "445 AO-SupportsTriggering": "0", "ROI": "0-0-1200-1200", "COM9-Verbose": "1", "PRIME-Color": "OFF", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "PRIME-ShutterOpenDelay": "0", "COM1-Parity": "None", "Slice": 9, "SlicePosition": 40, "COM9-DelayBetweenCharsMs": "0.0000", "555-Name": "DA Shutter", "PIEZO-LudlSingleAxisName": "B", "XLIGHT Emission Wheel-Label": "585/11(T)", "640 Shutter-Name": "AnalogIO", "640 AO-Sequenceable": "1", "520 Shutter-Demo": "No", "528 Shutter-Demo": "No", "470-Name": "DA Shutter", "520-DA Device": "520 Shutter", "XYStage-Description": "Ludl XY stage driver adapter", "445 Shutter-MinVolts": "0.0000", "528 AO-TriggerSampleRate": "100000", "405 AO-MinVolts": "0.0000", "405 Shutter-TriggerSequenceLength": "1000", "Height": 1200, "555 AO-Block voltage": "false", "640 Shutter-Volts": "0.0000", "PixelType": "GRAY16", "445 Shutter-Block voltage": "false", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "520 Shutter-TriggerSequenceLength": "1000", "COM9-Name": "COM9", "Core-SLM": "", "555 AO-Volts": "4.5000", "555 Shutter-Block voltage": "false", "XYStage-Speed": "1800.0000", "520 AO-MaxVolts": "5.0000", "528 Shutter-SupportsTriggering": "0", "COM1-Description": "Serial port driver (boost:asio)", "PRIME-MetadataEnabled": "No", "520 Shutter-MinVolts": "0.0000", "470 Shutter-Sequenceable": "1", "PRIME-PreampDelay": "0", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-MinVolts": "0.0000", "ChannelIndex": 1, "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Core-AutoShutter": "1", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "Exposure-ms": 160, "PRIME-PP 2 ENABLED": "Yes", "640 Shutter-TriggerSampleRate": "100000", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "470 Shutter-IOChannel": "Dev1/ao10", "405 AO-Demo": "No", "PixelSizeUm": 1.1, "470 AO-Demo": "No", "470 Shutter-MaxVolts": "5.0000", "Vincent-D3-Name": "Vincent-D3", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FileName": "img_000000000_Il-T_009.tif", "LudlController-Reset": "Operate", "PRIME-Exposure": "160.00", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "445-Name": "DA Shutter", "555 Shutter-Name": "AnalogIO", "520 Shutter-TriggerSampleRate": "100000", "445 Shutter-IOChannel": "Dev1/ao9", "PRIME-TransposeCorrection": "0", "XYStage-StepSize": "0.1000", "445-Description": "DA device that is used as a shutter", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 AO-IOChannel": "Dev1/ao7", "520 Shutter-Name": "AnalogIO", "COM3-DataBits": "8", "640 AO-Volts": "0.0000", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "555 Shutter-IOChannel": "Dev1/ao13", "WaitInterval": null, "520 Shutter-Sequenceable": "1", "YPositionUm": -13012.300000000001, "445-State": "0", "445-DA Device": "445 Shutter", "PRIME-CircularBufferAutoSize": "ON", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "555 AO-TriggerSampleRate": "100000", "445 Shutter-Volts": "0.0000", "XLIGHT Hub-Port": "COM3", "Core-AutoFocus": "", "COM4-Handshaking": "Off", "528 Shutter-MinVolts": "0.0000", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "445 Shutter-Name": "AnalogIO", "528 Shutter-Sequenceable": "1", "XYStage-TransposeMirrorX": "0", "XYStage-TransposeMirrorY": "0", "PRIME-ReadoutRate": "100MHz 16bit", "XYStage-Name": "Stage", "640 AO-SupportsTriggering": "0", "640-Name": "DA Shutter", "640 Shutter-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "640 AO-Block voltage": "false", "445 Shutter-Sequenceable": "1", "470 AO-MaxVolts": "5.0000", "COM4-BaudRate": "9600", "470-Description": "DA device that is used as a shutter", "520 Shutter-Description": "NI DAC", "PRIME-CCDTemperature": "-14.9200", "Time": "2019-12-17 03:59:43 -0500", "640 AO-TriggerSequenceLength": "1000", "470 AO-Name": "AnalogIO", "XLIGHT Disk slider-Label": "Disk Pos 40um", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "Binning": "1x1", "TE2000-ModelType": "1", "COM3-AnswerTimeout": "500.0000", "555 Shutter-SupportsTriggering": "0", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "TE2000-Port": "COM4", "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Description": "NI DAC", "COM4-AnswerTimeout": "500.0000", "COM1-DelayBetweenCharsMs": "0.0000", "FilterBlock-Label": "Confocal", "445 AO-Name": "AnalogIO", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-Timing-ReadoutTimeNs": "24672000.0000", "528 AO-Demo": "No", "520 AO-Sequenceable": "1", "555-Description": "DA device that is used as a shutter", "405-DA Device": "405 Shutter", "405 AO-TriggerSequenceLength": "1000", "640 AO-MinVolts": "0.0000", "640 Shutter-Block voltage": "false", "FrameIndex": 0, "PRIME-Port": "CMOS", "555 AO-MinVolts": "0.0000", "COM3-Description": "Serial port driver (boost:asio)", "528 AO-Description": "NI DAC", "555 AO-IOChannel": "Dev1/ao6", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "555 AO-TriggerInputLine": "/Dev1/PFI0", "PRIME-ActualInterval-ms": "84.6200", "LudlController-Port": "COM9", "445 Shutter-Demo": "No", "PRIME-Trigger-Expose Out-Mux": "1", "SliceIndex": 9, "PRIME-CCDTemperatureSetPoint": "-15.0000", "405 Shutter-MinVolts": "0.0000", "PRIME-ExposeOutMode": "First Row", "AxisPositions": null, "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Volts": "5.0000", "528-Name": "DA Shutter", "555-State": "1", "XLIGHT Dichroic-Label": "QUAD", "445 AO-Sequenceable": "1", "405-Name": "DA Shutter", "405 AO-IOChannel": "Dev1/ao0", "Core-Camera": "PRIME", "Focus-Name": "Focus", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "405 Shutter-IOChannel": "Dev1/ao8", "640 AO-Demo": "No", "640 Shutter-SupportsTriggering": "0", "445 AO-Demo": "No", "555-DA Device": "555 Shutter", "470 Shutter-Description": "NI DAC", "528 AO-Volts": "0.0000", "COM1-DataBits": "8", "Vincent-D3-Address": "x", "520 AO-SupportsTriggering": "0", "405-Description": "DA device that is used as a shutter", "640 Shutter-MinVolts": "0.0000", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "405 AO-Name": "AnalogIO", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "640 AO-TriggerInputLine": "/Dev1/PFI0", "405 AO-TriggerInputLine": "/Dev1/PFI0", "640-Description": "DA device that is used as a shutter", "445 Shutter-TriggerSampleRate": "100000", "470 Shutter-TriggerSequenceLength": "1000", "COM1-StopBits": "1", "520 Shutter-SupportsTriggering": "0", "405-State": "0", "Core-Initialize": "1", "XLIGHT Emission Wheel-State": "3", "520 AO-Description": "NI DAC", "528 AO-TriggerInputLine": "/Dev1/PFI0", "445 Shutter-MaxVolts": "5.0000", "COM9-Handshaking": "Off", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "XLIGHT Spin motor-Label": "On (spinning)", "PRIME-PP 5 ENABLED": "No", "UUID": "35ecd717-5942-48b3-9873-b97455cce0cc", "PRIME-TriggerMode": "Internal Trigger", "COM1-Handshaking": "Off", "PRIME-Timing-PostTriggerDelayNs": "49344000.0000", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "Focus-Position": "16732.9000", "528 Shutter-Block voltage": "false", "Channel": "Il-T", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "555 Shutter-Demo": "No", "405 Shutter-Demo": "No", "528 AO-Name": "AnalogIO", "520-State": "0", "Core-Galvo": "", "405 Shutter-TriggerSampleRate": "100000", "Focus-FocusStepSize_(nm)": "100", "PRIME-PixelType": "16bit", "COM4-DataBits": "8", "470 AO-IOChannel": "Dev1/ao2", "520 AO-MinVolts": "0.0000", "COM9-Parity": "None", "PRIME-CircularBufferEnabled": "ON", "PRIME-PP 6 Reset": "No", "640 AO-Description": "NI DAC", "Source": "PRIME", "445 AO-MinVolts": "0.0000", "PIEZO-Name": "Stage", "445 AO-Description": "NI DAC", "PRIME-CircularBufferFrameCount": "46", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "520 AO-Volts": "0.0000", "528 Shutter-TriggerSampleRate": "100000", "XPositionUm": 23269.100000000002, "OpticalPath-State": "4", "PRIME-PP 3 THRESHOLD": "80", "PRIME-PVCAM Version": "3.7.1", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Gain": "1-HDR", "640 Shutter-TriggerSequenceLength": "1000", "528 AO-SupportsTriggering": "0", "445 AO-TriggerInputLine": "/Dev1/PFI0", "OpticalPath-Name": "OpticalPath", "PRIME-PP 4 ENABLED": "Yes", "PRIME-PVCAM Adapter Version": "1.3.29", "COM3-Name": "COM3", "405 AO-MaxVolts": "5.0000", "520 Shutter-Block voltage": "false", "PRIME-Timing-ClearingTimeNs": "49344000.0000", "Core-Focus": "PIEZO", "528 AO-Block voltage": "false", "TE2000-Description": "Nikon TE2000 hub", "528 AO-MinVolts": "0.0000", "COM4-Parity": "None", "Summary": { "Slices": 11, "ChContrastMin": [ 0, 0, 0, 0, 0, 0 ], "PixelAspect": 1, "SlicesFirst": true, "Interval_ms": 0, "Time": "2019-12-17 03:59:33 -0500", "Prefix": "seq_7", "InitialPositionList": [ { "Label": "Pos0", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [149.91], "Focus": [16657.7], "XYStage": [ 71629.7, 19152.9 ] }, "GridColumnIndex": 0 }, { "Label": "Pos1", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [190.82], "Focus": [16485.4], "XYStage": [ 65702.3, 19396.600000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos2", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [194.69], "Focus": [16446.8], "XYStage": [ 59880.5, 19007.8 ] }, "GridColumnIndex": 0 }, { "Label": "Pos3", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [145.04], "Focus": [16446.6], "XYStage": [ 53616.9, 18818.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos4", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [169.57500000000002], "Focus": [16446.5], "XYStage": [ 47662.8, 18817.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos5", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [131.805], "Focus": [16446.4], "XYStage": [ 41918.8, 18298.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos6", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [113.145], "Focus": [16446.5], "XYStage": [ 36129.9, 18895.8 ] }, "GridColumnIndex": 0 }, { "Label": "Pos7", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [141.005], "Focus": [16446.6], "XYStage": [ 30299.100000000002, 18895.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos8", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [140.97], "Focus": [16446.5], "XYStage": [ 23952.100000000002, 19156 ] }, "GridColumnIndex": 0 }, { "Label": "Pos9", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [184.57500000000002], "Focus": [16677.9], "XYStage": [ 72866.8, -14809.300000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos10", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [167.95000000000002], "Focus": [16557.9], "XYStage": [ 66515.6, -14371.300000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos11", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [168], "Focus": [16618.9], "XYStage": [ 59724.3, -13964.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos12", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [203.205], "Focus": [16779.6], "XYStage": [ 53862.3, -13494.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos13", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [203.20000000000002], "Focus": [16749], "XYStage": [ 48108.3, -13805.400000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos14", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [203.19], "Focus": [16675.9], "XYStage": [ 41645, -13804.900000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos15", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [203.21], "Focus": [16675.7], "XYStage": [ 35352.6, -13805.6 ] }, "GridColumnIndex": 0 }, { "Label": "Pos16", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [203.225], "Focus": [16675.8], "XYStage": [ 29298, -13447.400000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos17", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [203.215], "Focus": [16675.8], "XYStage": [ 23269.100000000002, -13012.300000000001 ] }, "GridColumnIndex": 0 } ], "z-step_um": 10, "GridRow": 0, "BitDepth": 16, "Depth": 2, "Channels": 6, "Source": "Micro-Manager", "ChColors": [ -16711681, -256, -65281, -1, -1, -16776961 ], "MetadataVersion": 10, "KeepShutterOpenChannels": true, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536, 65536 ], "CustomIntervals_ms": [], "Height": 1200, "MicroManagerVersion": "1.4.23 20180219", "GridColumn": 0, "UUID": "3ad8801b-5699-40d6-813b-4ad840863e38", "PixelType": "GRAY16", "Width": 1200, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\Yan\\20191216-YC113-S11S12-miseq", "Frames": 1, "Comment": "", "UserName": "Nikon", "PixelSize_um": 1.1, "ROI": [ 0, 0, 1200, 1200 ], "Date": "2019-12-17", "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC", "P-DAPI-high" ], "KeepShutterOpenSlices": true, "IJType": 1, "ComputerName": "ZadorConfocal2", "Positions": 18, "PositionIndex": 17, "CameraTimeout": 20000, "TimeFirst": false }, "640 AO-Name": "AnalogIO", "Vincent-D3-Command": "Close" }, "FrameKey-0-1-10": { "COM4-StopBits": "1", "528 AO-MaxVolts": "5.0000", "XLIGHT Excitation Wheel-State": "1", "470 Shutter-Name": "AnalogIO", "Core-Shutter": "555", "555 AO-Demo": "No", "XLIGHT Dichroic-State": "2", "XYStage-StepSize-Y": "0.1000", "ZPositionUm": 253.215, "XYStage-StepSize-X": "0.1000", "PRIME-FirmwareVersion": "20.28", "PRIME-ClearCycles": "2", "520-Name": "DA Shutter", "405 Shutter-Name": "AnalogIO", "PIEZO-StepSize": "0.0050", "PRIME-ClearMode": "Pre-Exposure", "470 AO-MinVolts": "0.0000", "405 AO-SupportsTriggering": "1", "CameraChannelIndex": 0, "XYStage-StartSpeed": "500.0000", "COM9-StopBits": "2", "405 AO-Description": "NI DAC", "COM4-DelayBetweenCharsMs": "0.0000", "445 AO-Block voltage": "false", "520 AO-TriggerInputLine": "/Dev1/PFI0", "555 AO-Description": "NI DAC", "520 AO-TriggerSampleRate": "100000", "COM4-Verbose": "1", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM4-Name": "COM4", "405 Shutter-Volts": "0.0000", "Vincent-D3-Port": "COM1", "PRIME-PP 4 MIN ADU AFFECTED": "200", "470 Shutter-MinVolts": "0.0000", "PRIME-PP 3 ENABLED": "Yes", "470 AO-Description": "NI DAC", "PRIME-Description": "PVCAM API device adapter", "520 Shutter-MaxVolts": "5.0000", "520 Shutter-Volts": "0.0000", "528 AO-Sequenceable": "1", "PRIME-Binning": "1x1", "555 Shutter-MaxVolts": "5.0000", "528 Shutter-TriggerSequenceLength": "1000", "405 Shutter-Block voltage": "false", "XLIGHT Spin motor-State": "1", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "Vincent-D3-Time to open (ms)": "35.0000", "Vincent-D3-Time to close (ms)": "35.0000", "445 AO-TriggerSampleRate": "100000", "640 Shutter-IOChannel": "Dev1/ao14", "555 AO-Sequenceable": "1", "520 AO-Name": "AnalogIO", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "470 AO-Block voltage": "false", "COM1-Verbose": "1", "COM9-AnswerTimeout": "10000.0000", "528 Shutter-Name": "AnalogIO", "PRIME-Trigger Timeout (secs)": "10", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "NextFrame": 0, "470 AO-TriggerSequenceLength": "1000", "PRIME-TransposeXY": "0", "445 AO-TriggerSequenceLength": "1000", "COM1-Name": "COM1", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "COM9-DataBits": "8", "COM1-AnswerTimeout": "500.0000", "Camera": "", "TE2000-Version": "V2.50-V2.50-V2.10", "528 Shutter-Volts": "0.0000", "XLIGHT Hub-Name": "XLIGHT Hub", "PIEZO-Description": "Ludl stage driver adapter", "PositionName": "Pos17", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSampleRate": "100000", "PRIME-FanSpeedSetpoint": "High", "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "405 AO-TriggerSampleRate": "100000", "470 Shutter-Block voltage": "false", "470-State": "0", "COM3-Parity": "None", "405 AO-Volts": "0.0000", "PRIME-TransposeMirrorY": "0", "PRIME-ChipName": "GS144BSI", "470 AO-Volts": "0.0000", "PRIME-TransposeMirrorX": "0", "445 Shutter-SupportsTriggering": "0", "470 Shutter-SupportsTriggering": "0", "XLIGHT Hub-Description": "XLIGHT hub", "PRIME-PreampOffLimit": "10000", "470 AO-Sequenceable": "1", "528 Shutter-MaxVolts": "5.0000", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "Core-XYStage": "XYStage", "470 AO-TriggerSampleRate": "100000", "PRIME-Timing-PreTriggerDelayNs": "24672000.0000", "528-DA Device": "528 Shutter", "Focus-Description": "Nikon TE2000 focus adapter", "FilterBlock-State": "5", "520 AO-Demo": "No", "COM3-DelayBetweenCharsMs": "0.0000", "470 Shutter-Volts": "0.0000", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "PRIME-PP 2 THRESHOLD": "125", "PRIME-PP 4 THRESHOLD": "75", "XYStage-Acceleration": "4.0000", "470 Shutter-Demo": "No", "PRIME-Name": "PMPCIECam00", "640-DA Device": "640 Shutter", "405 AO-Block voltage": "false", "ElapsedTime-ms": 727292, "PRIME-Offset": "100", "445 AO-IOChannel": "Dev1/ao1", "640 AO-MaxVolts": "5.0000", "PositionIndex": 17, "PRIME-PP 1 ENABLED": "Yes", "COM1-BaudRate": "9600", "640-State": "0", "COM3-Verbose": "1", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "LudlController-TransmissionDelay": "0", "PRIME-FullWellCapacity": "62000", "PRIME-SMARTStreamingEnabled": "No", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "640 Shutter-Description": "NI DAC", "528-Description": "DA device that is used as a shutter", "COM4-Description": "Serial port driver (boost:asio)", "405 Shutter-Description": "NI DAC", "TE2000-Name": "TE2000", "528 Shutter-Description": "NI DAC", "COM9-Description": "Serial port driver (boost:asio)", "LudlController-Name": "LudlController", "445 Shutter-TriggerSequenceLength": "1000", "PRIME-SerialNumber": "A18B203008", "445 AO-Volts": "0.0000", "PRIME-X-dimension": "1200", "PRIME-ShutterCloseDelay": "0", "XLIGHT Disk slider-State": "2", "Position": "Default", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 AO-IOChannel": "Dev1/ao5", "520 AO-IOChannel": "Dev1/ao4", "528 AO-TriggerSequenceLength": "1000", "405 Shutter-MaxVolts": "5.0000", "470-DA Device": "470 Shutter", "PRIME-FTCapable": "No", "Core-ChannelGroup": "Channels", "555 AO-SupportsTriggering": "0", "PRIME-Y-dimension": "1200", "Frame": 0, "555 Shutter-Sequenceable": "1", "PRIME-Timing-ExposureTimeNs": "499998000.0000", "Core-ImageProcessor": "", "528-State": "0", "520 AO-Block voltage": "false", "XLIGHT Excitation Wheel-Label": "QUAD", "640 Shutter-Demo": "No", "COM3-StopBits": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "520 Shutter-IOChannel": "Dev1/ao11", "Width": 1200, "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-SupportsTriggering": "0", "640 AO-TriggerSampleRate": "100000", "520-Description": "DA device that is used as a shutter", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "405 Shutter-Sequenceable": "1", "405 Shutter-SupportsTriggering": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-TriggerSampleRate": "100000", "640 Shutter-MaxVolts": "5.0000", "555 AO-MaxVolts": "5.0000", "520 AO-TriggerSequenceLength": "1000", "COM3-BaudRate": "9600", "COM9-BaudRate": "9600", "Vincent-D3-Channel #": "Ch. #1", "PRIME-PMode": "Normal", "OpticalPath-Label": "Left(100)", "BitDepth": 16, "555 AO-TriggerSequenceLength": "1000", "445 Shutter-Description": "NI DAC", "405 AO-Sequenceable": "1", "PRIME-AcquisitionMethod": "Callbacks", "555 AO-Name": "AnalogIO", "PRIME-ShutterMode": "Pre-Exposure", "COM3-Handshaking": "Off", "445 AO-SupportsTriggering": "0", "ROI": "0-0-1200-1200", "COM9-Verbose": "1", "PRIME-Color": "OFF", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "PRIME-ShutterOpenDelay": "0", "COM1-Parity": "None", "Slice": 10, "SlicePosition": 50, "COM9-DelayBetweenCharsMs": "0.0000", "555-Name": "DA Shutter", "PIEZO-LudlSingleAxisName": "B", "XLIGHT Emission Wheel-Label": "585/11(T)", "640 Shutter-Name": "AnalogIO", "640 AO-Sequenceable": "1", "520 Shutter-Demo": "No", "528 Shutter-Demo": "No", "470-Name": "DA Shutter", "520-DA Device": "520 Shutter", "XYStage-Description": "Ludl XY stage driver adapter", "445 Shutter-MinVolts": "0.0000", "528 AO-TriggerSampleRate": "100000", "405 AO-MinVolts": "0.0000", "405 Shutter-TriggerSequenceLength": "1000", "Height": 1200, "555 AO-Block voltage": "false", "640 Shutter-Volts": "0.0000", "PixelType": "GRAY16", "445 Shutter-Block voltage": "false", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "520 Shutter-TriggerSequenceLength": "1000", "COM9-Name": "COM9", "Core-SLM": "", "555 AO-Volts": "4.5000", "555 Shutter-Block voltage": "false", "XYStage-Speed": "1800.0000", "520 AO-MaxVolts": "5.0000", "528 Shutter-SupportsTriggering": "0", "COM1-Description": "Serial port driver (boost:asio)", "PRIME-MetadataEnabled": "No", "520 Shutter-MinVolts": "0.0000", "470 Shutter-Sequenceable": "1", "PRIME-PreampDelay": "0", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-MinVolts": "0.0000", "ChannelIndex": 1, "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Core-AutoShutter": "1", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "Exposure-ms": 160, "PRIME-PP 2 ENABLED": "Yes", "640 Shutter-TriggerSampleRate": "100000", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "470 Shutter-IOChannel": "Dev1/ao10", "405 AO-Demo": "No", "PixelSizeUm": 1.1, "470 AO-Demo": "No", "470 Shutter-MaxVolts": "5.0000", "Vincent-D3-Name": "Vincent-D3", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FileName": "img_000000000_Il-T_010.tif", "LudlController-Reset": "Operate", "PRIME-Exposure": "160.00", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "445-Name": "DA Shutter", "555 Shutter-Name": "AnalogIO", "520 Shutter-TriggerSampleRate": "100000", "445 Shutter-IOChannel": "Dev1/ao9", "PRIME-TransposeCorrection": "0", "XYStage-StepSize": "0.1000", "445-Description": "DA device that is used as a shutter", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 AO-IOChannel": "Dev1/ao7", "520 Shutter-Name": "AnalogIO", "COM3-DataBits": "8", "640 AO-Volts": "0.0000", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "555 Shutter-IOChannel": "Dev1/ao13", "WaitInterval": null, "520 Shutter-Sequenceable": "1", "YPositionUm": -13012.300000000001, "445-State": "0", "445-DA Device": "445 Shutter", "PRIME-CircularBufferAutoSize": "ON", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "555 AO-TriggerSampleRate": "100000", "445 Shutter-Volts": "0.0000", "XLIGHT Hub-Port": "COM3", "Core-AutoFocus": "", "COM4-Handshaking": "Off", "528 Shutter-MinVolts": "0.0000", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "445 Shutter-Name": "AnalogIO", "528 Shutter-Sequenceable": "1", "XYStage-TransposeMirrorX": "0", "XYStage-TransposeMirrorY": "0", "PRIME-ReadoutRate": "100MHz 16bit", "XYStage-Name": "Stage", "640 AO-SupportsTriggering": "0", "640-Name": "DA Shutter", "640 Shutter-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "640 AO-Block voltage": "false", "445 Shutter-Sequenceable": "1", "470 AO-MaxVolts": "5.0000", "COM4-BaudRate": "9600", "470-Description": "DA device that is used as a shutter", "520 Shutter-Description": "NI DAC", "PRIME-CCDTemperature": "-14.9200", "Time": "2019-12-17 03:59:44 -0500", "640 AO-TriggerSequenceLength": "1000", "470 AO-Name": "AnalogIO", "XLIGHT Disk slider-Label": "Disk Pos 40um", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "Binning": "1x1", "TE2000-ModelType": "1", "COM3-AnswerTimeout": "500.0000", "555 Shutter-SupportsTriggering": "0", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "TE2000-Port": "COM4", "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Description": "NI DAC", "COM4-AnswerTimeout": "500.0000", "COM1-DelayBetweenCharsMs": "0.0000", "FilterBlock-Label": "Confocal", "445 AO-Name": "AnalogIO", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-Timing-ReadoutTimeNs": "24672000.0000", "528 AO-Demo": "No", "520 AO-Sequenceable": "1", "555-Description": "DA device that is used as a shutter", "405-DA Device": "405 Shutter", "405 AO-TriggerSequenceLength": "1000", "640 AO-MinVolts": "0.0000", "640 Shutter-Block voltage": "false", "FrameIndex": 0, "PRIME-Port": "CMOS", "555 AO-MinVolts": "0.0000", "COM3-Description": "Serial port driver (boost:asio)", "528 AO-Description": "NI DAC", "555 AO-IOChannel": "Dev1/ao6", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "555 AO-TriggerInputLine": "/Dev1/PFI0", "PRIME-ActualInterval-ms": "84.6200", "LudlController-Port": "COM9", "445 Shutter-Demo": "No", "PRIME-Trigger-Expose Out-Mux": "1", "SliceIndex": 10, "PRIME-CCDTemperatureSetPoint": "-15.0000", "405 Shutter-MinVolts": "0.0000", "PRIME-ExposeOutMode": "First Row", "AxisPositions": null, "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Volts": "5.0000", "528-Name": "DA Shutter", "555-State": "0", "XLIGHT Dichroic-Label": "QUAD", "445 AO-Sequenceable": "1", "405-Name": "DA Shutter", "405 AO-IOChannel": "Dev1/ao0", "Core-Camera": "PRIME", "Focus-Name": "Focus", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "405 Shutter-IOChannel": "Dev1/ao8", "640 AO-Demo": "No", "640 Shutter-SupportsTriggering": "0", "445 AO-Demo": "No", "555-DA Device": "555 Shutter", "470 Shutter-Description": "NI DAC", "528 AO-Volts": "0.0000", "COM1-DataBits": "8", "Vincent-D3-Address": "x", "520 AO-SupportsTriggering": "0", "405-Description": "DA device that is used as a shutter", "640 Shutter-MinVolts": "0.0000", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "405 AO-Name": "AnalogIO", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "640 AO-TriggerInputLine": "/Dev1/PFI0", "405 AO-TriggerInputLine": "/Dev1/PFI0", "640-Description": "DA device that is used as a shutter", "445 Shutter-TriggerSampleRate": "100000", "470 Shutter-TriggerSequenceLength": "1000", "COM1-StopBits": "1", "520 Shutter-SupportsTriggering": "0", "405-State": "0", "Core-Initialize": "1", "XLIGHT Emission Wheel-State": "3", "520 AO-Description": "NI DAC", "528 AO-TriggerInputLine": "/Dev1/PFI0", "445 Shutter-MaxVolts": "5.0000", "COM9-Handshaking": "Off", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "XLIGHT Spin motor-Label": "On (spinning)", "PRIME-PP 5 ENABLED": "No", "UUID": "feb73d30-966f-4b7d-b138-ec173b80ab0d", "PRIME-TriggerMode": "Internal Trigger", "COM1-Handshaking": "Off", "PRIME-Timing-PostTriggerDelayNs": "49344000.0000", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "Focus-Position": "16732.9000", "528 Shutter-Block voltage": "false", "Channel": "Il-T", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "555 Shutter-Demo": "No", "405 Shutter-Demo": "No", "528 AO-Name": "AnalogIO", "520-State": "0", "Core-Galvo": "", "405 Shutter-TriggerSampleRate": "100000", "Focus-FocusStepSize_(nm)": "100", "PRIME-PixelType": "16bit", "COM4-DataBits": "8", "470 AO-IOChannel": "Dev1/ao2", "520 AO-MinVolts": "0.0000", "COM9-Parity": "None", "PRIME-CircularBufferEnabled": "ON", "PRIME-PP 6 Reset": "No", "640 AO-Description": "NI DAC", "Source": "PRIME", "445 AO-MinVolts": "0.0000", "PIEZO-Name": "Stage", "445 AO-Description": "NI DAC", "PRIME-CircularBufferFrameCount": "46", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "520 AO-Volts": "0.0000", "528 Shutter-TriggerSampleRate": "100000", "XPositionUm": 23269.100000000002, "OpticalPath-State": "4", "PRIME-PP 3 THRESHOLD": "80", "PRIME-PVCAM Version": "3.7.1", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Gain": "1-HDR", "640 Shutter-TriggerSequenceLength": "1000", "528 AO-SupportsTriggering": "0", "445 AO-TriggerInputLine": "/Dev1/PFI0", "OpticalPath-Name": "OpticalPath", "PRIME-PP 4 ENABLED": "Yes", "PRIME-PVCAM Adapter Version": "1.3.29", "COM3-Name": "COM3", "405 AO-MaxVolts": "5.0000", "520 Shutter-Block voltage": "false", "PRIME-Timing-ClearingTimeNs": "49344000.0000", "Core-Focus": "PIEZO", "528 AO-Block voltage": "false", "TE2000-Description": "Nikon TE2000 hub", "528 AO-MinVolts": "0.0000", "COM4-Parity": "None", "Summary": { "Slices": 11, "ChContrastMin": [ 0, 0, 0, 0, 0, 0 ], "PixelAspect": 1, "SlicesFirst": true, "Interval_ms": 0, "Time": "2019-12-17 03:59:33 -0500", "Prefix": "seq_7", "InitialPositionList": [ { "Label": "Pos0", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [149.91], "Focus": [16657.7], "XYStage": [ 71629.7, 19152.9 ] }, "GridColumnIndex": 0 }, { "Label": "Pos1", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [190.82], "Focus": [16485.4], "XYStage": [ 65702.3, 19396.600000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos2", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [194.69], "Focus": [16446.8], "XYStage": [ 59880.5, 19007.8 ] }, "GridColumnIndex": 0 }, { "Label": "Pos3", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [145.04], "Focus": [16446.6], "XYStage": [ 53616.9, 18818.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos4", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [169.57500000000002], "Focus": [16446.5], "XYStage": [ 47662.8, 18817.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos5", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [131.805], "Focus": [16446.4], "XYStage": [ 41918.8, 18298.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos6", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [113.145], "Focus": [16446.5], "XYStage": [ 36129.9, 18895.8 ] }, "GridColumnIndex": 0 }, { "Label": "Pos7", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [141.005], "Focus": [16446.6], "XYStage": [ 30299.100000000002, 18895.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos8", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [140.97], "Focus": [16446.5], "XYStage": [ 23952.100000000002, 19156 ] }, "GridColumnIndex": 0 }, { "Label": "Pos9", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [184.57500000000002], "Focus": [16677.9], "XYStage": [ 72866.8, -14809.300000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos10", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [167.95000000000002], "Focus": [16557.9], "XYStage": [ 66515.6, -14371.300000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos11", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [168], "Focus": [16618.9], "XYStage": [ 59724.3, -13964.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos12", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [203.205], "Focus": [16779.6], "XYStage": [ 53862.3, -13494.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos13", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [203.20000000000002], "Focus": [16749], "XYStage": [ 48108.3, -13805.400000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos14", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [203.19], "Focus": [16675.9], "XYStage": [ 41645, -13804.900000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos15", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [203.21], "Focus": [16675.7], "XYStage": [ 35352.6, -13805.6 ] }, "GridColumnIndex": 0 }, { "Label": "Pos16", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [203.225], "Focus": [16675.8], "XYStage": [ 29298, -13447.400000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos17", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [203.215], "Focus": [16675.8], "XYStage": [ 23269.100000000002, -13012.300000000001 ] }, "GridColumnIndex": 0 } ], "z-step_um": 10, "GridRow": 0, "BitDepth": 16, "Depth": 2, "Channels": 6, "Source": "Micro-Manager", "ChColors": [ -16711681, -256, -65281, -1, -1, -16776961 ], "MetadataVersion": 10, "KeepShutterOpenChannels": true, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536, 65536 ], "CustomIntervals_ms": [], "Height": 1200, "MicroManagerVersion": "1.4.23 20180219", "GridColumn": 0, "UUID": "3ad8801b-5699-40d6-813b-4ad840863e38", "PixelType": "GRAY16", "Width": 1200, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\Yan\\20191216-YC113-S11S12-miseq", "Frames": 1, "Comment": "", "UserName": "Nikon", "PixelSize_um": 1.1, "ROI": [ 0, 0, 1200, 1200 ], "Date": "2019-12-17", "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC", "P-DAPI-high" ], "KeepShutterOpenSlices": true, "IJType": 1, "ComputerName": "ZadorConfocal2", "Positions": 18, "PositionIndex": 17, "CameraTimeout": 20000, "TimeFirst": false }, "640 AO-Name": "AnalogIO", "Vincent-D3-Command": "Close" }, "FrameKey-0-2-0": { "COM4-StopBits": "1", "528 AO-MaxVolts": "5.0000", "XLIGHT Excitation Wheel-State": "1", "470 Shutter-Name": "AnalogIO", "Core-Shutter": "640", "555 AO-Demo": "No", "XLIGHT Dichroic-State": "2", "XYStage-StepSize-Y": "0.1000", "ZPositionUm": 153.215, "XYStage-StepSize-X": "0.1000", "PRIME-FirmwareVersion": "20.28", "PRIME-ClearCycles": "2", "520-Name": "DA Shutter", "405 Shutter-Name": "AnalogIO", "PIEZO-StepSize": "0.0050", "PRIME-ClearMode": "Pre-Exposure", "470 AO-MinVolts": "0.0000", "405 AO-SupportsTriggering": "1", "CameraChannelIndex": 0, "XYStage-StartSpeed": "500.0000", "COM9-StopBits": "2", "405 AO-Description": "NI DAC", "COM4-DelayBetweenCharsMs": "0.0000", "445 AO-Block voltage": "false", "520 AO-TriggerInputLine": "/Dev1/PFI0", "555 AO-Description": "NI DAC", "520 AO-TriggerSampleRate": "100000", "COM4-Verbose": "1", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM4-Name": "COM4", "405 Shutter-Volts": "0.0000", "Vincent-D3-Port": "COM1", "PRIME-PP 4 MIN ADU AFFECTED": "200", "470 Shutter-MinVolts": "0.0000", "PRIME-PP 3 ENABLED": "Yes", "470 AO-Description": "NI DAC", "PRIME-Description": "PVCAM API device adapter", "520 Shutter-MaxVolts": "5.0000", "520 Shutter-Volts": "0.0000", "528 AO-Sequenceable": "1", "PRIME-Binning": "1x1", "555 Shutter-MaxVolts": "5.0000", "528 Shutter-TriggerSequenceLength": "1000", "405 Shutter-Block voltage": "false", "XLIGHT Spin motor-State": "1", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "Vincent-D3-Time to open (ms)": "35.0000", "Vincent-D3-Time to close (ms)": "35.0000", "445 AO-TriggerSampleRate": "100000", "640 Shutter-IOChannel": "Dev1/ao14", "555 AO-Sequenceable": "1", "520 AO-Name": "AnalogIO", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "470 AO-Block voltage": "false", "COM1-Verbose": "1", "COM9-AnswerTimeout": "10000.0000", "528 Shutter-Name": "AnalogIO", "PRIME-Trigger Timeout (secs)": "10", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "NextFrame": 0, "470 AO-TriggerSequenceLength": "1000", "PRIME-TransposeXY": "0", "445 AO-TriggerSequenceLength": "1000", "COM1-Name": "COM1", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "COM9-DataBits": "8", "COM1-AnswerTimeout": "500.0000", "Camera": "", "TE2000-Version": "V2.50-V2.50-V2.10", "528 Shutter-Volts": "0.0000", "XLIGHT Hub-Name": "XLIGHT Hub", "PIEZO-Description": "Ludl stage driver adapter", "PositionName": "Pos17", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSampleRate": "100000", "PRIME-FanSpeedSetpoint": "High", "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "405 AO-TriggerSampleRate": "100000", "470 Shutter-Block voltage": "false", "470-State": "0", "COM3-Parity": "None", "405 AO-Volts": "0.0000", "PRIME-TransposeMirrorY": "0", "PRIME-ChipName": "GS144BSI", "470 AO-Volts": "0.0000", "PRIME-TransposeMirrorX": "0", "445 Shutter-SupportsTriggering": "0", "470 Shutter-SupportsTriggering": "0", "XLIGHT Hub-Description": "XLIGHT hub", "PRIME-PreampOffLimit": "10000", "470 AO-Sequenceable": "1", "528 Shutter-MaxVolts": "5.0000", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "Core-XYStage": "XYStage", "470 AO-TriggerSampleRate": "100000", "PRIME-Timing-PreTriggerDelayNs": "24672000.0000", "528-DA Device": "528 Shutter", "Focus-Description": "Nikon TE2000 focus adapter", "FilterBlock-State": "5", "520 AO-Demo": "No", "COM3-DelayBetweenCharsMs": "0.0000", "470 Shutter-Volts": "0.0000", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "PRIME-PP 2 THRESHOLD": "125", "PRIME-PP 4 THRESHOLD": "75", "XYStage-Acceleration": "4.0000", "470 Shutter-Demo": "No", "PRIME-Name": "PMPCIECam00", "640-DA Device": "640 Shutter", "405 AO-Block voltage": "false", "ElapsedTime-ms": 728415, "PRIME-Offset": "100", "445 AO-IOChannel": "Dev1/ao1", "640 AO-MaxVolts": "5.0000", "PositionIndex": 17, "PRIME-PP 1 ENABLED": "Yes", "COM1-BaudRate": "9600", "640-State": "1", "COM3-Verbose": "1", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "LudlController-TransmissionDelay": "0", "PRIME-FullWellCapacity": "62000", "PRIME-SMARTStreamingEnabled": "No", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "640 Shutter-Description": "NI DAC", "528-Description": "DA device that is used as a shutter", "COM4-Description": "Serial port driver (boost:asio)", "405 Shutter-Description": "NI DAC", "TE2000-Name": "TE2000", "528 Shutter-Description": "NI DAC", "COM9-Description": "Serial port driver (boost:asio)", "LudlController-Name": "LudlController", "445 Shutter-TriggerSequenceLength": "1000", "PRIME-SerialNumber": "A18B203008", "445 AO-Volts": "0.0000", "PRIME-X-dimension": "1200", "PRIME-ShutterCloseDelay": "0", "XLIGHT Disk slider-State": "2", "Position": "Default", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 AO-IOChannel": "Dev1/ao5", "520 AO-IOChannel": "Dev1/ao4", "528 AO-TriggerSequenceLength": "1000", "405 Shutter-MaxVolts": "5.0000", "470-DA Device": "470 Shutter", "PRIME-FTCapable": "No", "Core-ChannelGroup": "Channels", "555 AO-SupportsTriggering": "0", "PRIME-Y-dimension": "1200", "Frame": 0, "555 Shutter-Sequenceable": "1", "PRIME-Timing-ExposureTimeNs": "499998000.0000", "Core-ImageProcessor": "", "528-State": "0", "520 AO-Block voltage": "false", "XLIGHT Excitation Wheel-Label": "QUAD", "640 Shutter-Demo": "No", "COM3-StopBits": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "520 Shutter-IOChannel": "Dev1/ao11", "Width": 1200, "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-SupportsTriggering": "0", "640 AO-TriggerSampleRate": "100000", "520-Description": "DA device that is used as a shutter", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "405 Shutter-Sequenceable": "1", "405 Shutter-SupportsTriggering": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-TriggerSampleRate": "100000", "640 Shutter-MaxVolts": "5.0000", "555 AO-MaxVolts": "5.0000", "520 AO-TriggerSequenceLength": "1000", "COM3-BaudRate": "9600", "COM9-BaudRate": "9600", "Vincent-D3-Channel #": "Ch. #1", "PRIME-PMode": "Normal", "OpticalPath-Label": "Left(100)", "BitDepth": 16, "555 AO-TriggerSequenceLength": "1000", "445 Shutter-Description": "NI DAC", "405 AO-Sequenceable": "1", "PRIME-AcquisitionMethod": "Callbacks", "555 AO-Name": "AnalogIO", "PRIME-ShutterMode": "Pre-Exposure", "COM3-Handshaking": "Off", "445 AO-SupportsTriggering": "0", "ROI": "0-0-1200-1200", "COM9-Verbose": "1", "PRIME-Color": "OFF", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "PRIME-ShutterOpenDelay": "0", "COM1-Parity": "None", "Slice": 0, "SlicePosition": -50, "COM9-DelayBetweenCharsMs": "0.0000", "555-Name": "DA Shutter", "PIEZO-LudlSingleAxisName": "B", "XLIGHT Emission Wheel-Label": "679/29 (A)", "640 Shutter-Name": "AnalogIO", "640 AO-Sequenceable": "1", "520 Shutter-Demo": "No", "528 Shutter-Demo": "No", "470-Name": "DA Shutter", "520-DA Device": "520 Shutter", "XYStage-Description": "Ludl XY stage driver adapter", "445 Shutter-MinVolts": "0.0000", "528 AO-TriggerSampleRate": "100000", "405 AO-MinVolts": "0.0000", "405 Shutter-TriggerSequenceLength": "1000", "Height": 1200, "555 AO-Block voltage": "false", "640 Shutter-Volts": "5.0000", "PixelType": "GRAY16", "445 Shutter-Block voltage": "false", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "520 Shutter-TriggerSequenceLength": "1000", "COM9-Name": "COM9", "Core-SLM": "", "555 AO-Volts": "0.0000", "555 Shutter-Block voltage": "false", "XYStage-Speed": "1800.0000", "520 AO-MaxVolts": "5.0000", "528 Shutter-SupportsTriggering": "0", "COM1-Description": "Serial port driver (boost:asio)", "PRIME-MetadataEnabled": "No", "520 Shutter-MinVolts": "0.0000", "470 Shutter-Sequenceable": "1", "PRIME-PreampDelay": "0", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-MinVolts": "0.0000", "ChannelIndex": 2, "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Core-AutoShutter": "1", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "Exposure-ms": 320, "PRIME-PP 2 ENABLED": "Yes", "640 Shutter-TriggerSampleRate": "100000", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "470 Shutter-IOChannel": "Dev1/ao10", "405 AO-Demo": "No", "PixelSizeUm": 1.1, "470 AO-Demo": "No", "470 Shutter-MaxVolts": "5.0000", "Vincent-D3-Name": "Vincent-D3", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FileName": "img_000000000_Il-A_000.tif", "LudlController-Reset": "Operate", "PRIME-Exposure": "320.00", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "445-Name": "DA Shutter", "555 Shutter-Name": "AnalogIO", "520 Shutter-TriggerSampleRate": "100000", "445 Shutter-IOChannel": "Dev1/ao9", "PRIME-TransposeCorrection": "0", "XYStage-StepSize": "0.1000", "445-Description": "DA device that is used as a shutter", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 AO-IOChannel": "Dev1/ao7", "520 Shutter-Name": "AnalogIO", "COM3-DataBits": "8", "640 AO-Volts": "4.5000", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "555 Shutter-IOChannel": "Dev1/ao13", "WaitInterval": null, "520 Shutter-Sequenceable": "1", "YPositionUm": -13012.300000000001, "445-State": "0", "445-DA Device": "445 Shutter", "PRIME-CircularBufferAutoSize": "ON", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "555 AO-TriggerSampleRate": "100000", "445 Shutter-Volts": "0.0000", "XLIGHT Hub-Port": "COM3", "Core-AutoFocus": "", "COM4-Handshaking": "Off", "528 Shutter-MinVolts": "0.0000", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "445 Shutter-Name": "AnalogIO", "528 Shutter-Sequenceable": "1", "XYStage-TransposeMirrorX": "0", "XYStage-TransposeMirrorY": "0", "PRIME-ReadoutRate": "100MHz 16bit", "XYStage-Name": "Stage", "640 AO-SupportsTriggering": "0", "640-Name": "DA Shutter", "640 Shutter-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "640 AO-Block voltage": "false", "445 Shutter-Sequenceable": "1", "470 AO-MaxVolts": "5.0000", "COM4-BaudRate": "9600", "470-Description": "DA device that is used as a shutter", "520 Shutter-Description": "NI DAC", "PRIME-CCDTemperature": "-14.9200", "Time": "2019-12-17 03:59:45 -0500", "640 AO-TriggerSequenceLength": "1000", "470 AO-Name": "AnalogIO", "XLIGHT Disk slider-Label": "Disk Pos 40um", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "Binning": "1x1", "TE2000-ModelType": "1", "COM3-AnswerTimeout": "500.0000", "555 Shutter-SupportsTriggering": "0", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "TE2000-Port": "COM4", "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Description": "NI DAC", "COM4-AnswerTimeout": "500.0000", "COM1-DelayBetweenCharsMs": "0.0000", "FilterBlock-Label": "Confocal", "445 AO-Name": "AnalogIO", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-Timing-ReadoutTimeNs": "24672000.0000", "528 AO-Demo": "No", "520 AO-Sequenceable": "1", "555-Description": "DA device that is used as a shutter", "405-DA Device": "405 Shutter", "405 AO-TriggerSequenceLength": "1000", "640 AO-MinVolts": "0.0000", "640 Shutter-Block voltage": "false", "FrameIndex": 0, "PRIME-Port": "CMOS", "555 AO-MinVolts": "0.0000", "COM3-Description": "Serial port driver (boost:asio)", "528 AO-Description": "NI DAC", "555 AO-IOChannel": "Dev1/ao6", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "555 AO-TriggerInputLine": "/Dev1/PFI0", "PRIME-ActualInterval-ms": "84.6200", "LudlController-Port": "COM9", "445 Shutter-Demo": "No", "PRIME-Trigger-Expose Out-Mux": "1", "SliceIndex": 0, "PRIME-CCDTemperatureSetPoint": "-15.0000", "405 Shutter-MinVolts": "0.0000", "PRIME-ExposeOutMode": "First Row", "AxisPositions": null, "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Volts": "0.0000", "528-Name": "DA Shutter", "555-State": "0", "XLIGHT Dichroic-Label": "Di03-R635-t1", "445 AO-Sequenceable": "1", "405-Name": "DA Shutter", "405 AO-IOChannel": "Dev1/ao0", "Core-Camera": "PRIME", "Focus-Name": "Focus", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "405 Shutter-IOChannel": "Dev1/ao8", "640 AO-Demo": "No", "640 Shutter-SupportsTriggering": "0", "445 AO-Demo": "No", "555-DA Device": "555 Shutter", "470 Shutter-Description": "NI DAC", "528 AO-Volts": "0.0000", "COM1-DataBits": "8", "Vincent-D3-Address": "x", "520 AO-SupportsTriggering": "0", "405-Description": "DA device that is used as a shutter", "640 Shutter-MinVolts": "0.0000", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "405 AO-Name": "AnalogIO", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "640 AO-TriggerInputLine": "/Dev1/PFI0", "405 AO-TriggerInputLine": "/Dev1/PFI0", "640-Description": "DA device that is used as a shutter", "445 Shutter-TriggerSampleRate": "100000", "470 Shutter-TriggerSequenceLength": "1000", "COM1-StopBits": "1", "520 Shutter-SupportsTriggering": "0", "405-State": "0", "Core-Initialize": "1", "XLIGHT Emission Wheel-State": "3", "520 AO-Description": "NI DAC", "528 AO-TriggerInputLine": "/Dev1/PFI0", "445 Shutter-MaxVolts": "5.0000", "COM9-Handshaking": "Off", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "XLIGHT Spin motor-Label": "On (spinning)", "PRIME-PP 5 ENABLED": "No", "UUID": "6008756e-4abb-4126-bcdb-58792328fdcd", "PRIME-TriggerMode": "Internal Trigger", "COM1-Handshaking": "Off", "PRIME-Timing-PostTriggerDelayNs": "49344000.0000", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "Focus-Position": "16732.9000", "528 Shutter-Block voltage": "false", "Channel": "Il-A", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "555 Shutter-Demo": "No", "405 Shutter-Demo": "No", "528 AO-Name": "AnalogIO", "520-State": "0", "Core-Galvo": "", "405 Shutter-TriggerSampleRate": "100000", "Focus-FocusStepSize_(nm)": "100", "PRIME-PixelType": "16bit", "COM4-DataBits": "8", "470 AO-IOChannel": "Dev1/ao2", "520 AO-MinVolts": "0.0000", "COM9-Parity": "None", "PRIME-CircularBufferEnabled": "ON", "PRIME-PP 6 Reset": "No", "640 AO-Description": "NI DAC", "Source": "PRIME", "445 AO-MinVolts": "0.0000", "PIEZO-Name": "Stage", "445 AO-Description": "NI DAC", "PRIME-CircularBufferFrameCount": "46", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "520 AO-Volts": "0.0000", "528 Shutter-TriggerSampleRate": "100000", "XPositionUm": 23269.100000000002, "OpticalPath-State": "4", "PRIME-PP 3 THRESHOLD": "80", "PRIME-PVCAM Version": "3.7.1", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Gain": "1-HDR", "640 Shutter-TriggerSequenceLength": "1000", "528 AO-SupportsTriggering": "0", "445 AO-TriggerInputLine": "/Dev1/PFI0", "OpticalPath-Name": "OpticalPath", "PRIME-PP 4 ENABLED": "Yes", "PRIME-PVCAM Adapter Version": "1.3.29", "COM3-Name": "COM3", "405 AO-MaxVolts": "5.0000", "520 Shutter-Block voltage": "false", "PRIME-Timing-ClearingTimeNs": "49344000.0000", "Core-Focus": "PIEZO", "528 AO-Block voltage": "false", "TE2000-Description": "Nikon TE2000 hub", "528 AO-MinVolts": "0.0000", "COM4-Parity": "None", "Summary": { "Slices": 11, "ChContrastMin": [ 0, 0, 0, 0, 0, 0 ], "PixelAspect": 1, "SlicesFirst": true, "Interval_ms": 0, "Time": "2019-12-17 03:59:33 -0500", "Prefix": "seq_7", "InitialPositionList": [ { "Label": "Pos0", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [149.91], "Focus": [16657.7], "XYStage": [ 71629.7, 19152.9 ] }, "GridColumnIndex": 0 }, { "Label": "Pos1", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [190.82], "Focus": [16485.4], "XYStage": [ 65702.3, 19396.600000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos2", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [194.69], "Focus": [16446.8], "XYStage": [ 59880.5, 19007.8 ] }, "GridColumnIndex": 0 }, { "Label": "Pos3", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [145.04], "Focus": [16446.6], "XYStage": [ 53616.9, 18818.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos4", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [169.57500000000002], "Focus": [16446.5], "XYStage": [ 47662.8, 18817.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos5", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [131.805], "Focus": [16446.4], "XYStage": [ 41918.8, 18298.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos6", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [113.145], "Focus": [16446.5], "XYStage": [ 36129.9, 18895.8 ] }, "GridColumnIndex": 0 }, { "Label": "Pos7", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [141.005], "Focus": [16446.6], "XYStage": [ 30299.100000000002, 18895.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos8", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [140.97], "Focus": [16446.5], "XYStage": [ 23952.100000000002, 19156 ] }, "GridColumnIndex": 0 }, { "Label": "Pos9", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [184.57500000000002], "Focus": [16677.9], "XYStage": [ 72866.8, -14809.300000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos10", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [167.95000000000002], "Focus": [16557.9], "XYStage": [ 66515.6, -14371.300000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos11", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [168], "Focus": [16618.9], "XYStage": [ 59724.3, -13964.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos12", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [203.205], "Focus": [16779.6], "XYStage": [ 53862.3, -13494.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos13", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [203.20000000000002], "Focus": [16749], "XYStage": [ 48108.3, -13805.400000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos14", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [203.19], "Focus": [16675.9], "XYStage": [ 41645, -13804.900000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos15", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [203.21], "Focus": [16675.7], "XYStage": [ 35352.6, -13805.6 ] }, "GridColumnIndex": 0 }, { "Label": "Pos16", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [203.225], "Focus": [16675.8], "XYStage": [ 29298, -13447.400000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos17", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [203.215], "Focus": [16675.8], "XYStage": [ 23269.100000000002, -13012.300000000001 ] }, "GridColumnIndex": 0 } ], "z-step_um": 10, "GridRow": 0, "BitDepth": 16, "Depth": 2, "Channels": 6, "Source": "Micro-Manager", "ChColors": [ -16711681, -256, -65281, -1, -1, -16776961 ], "MetadataVersion": 10, "KeepShutterOpenChannels": true, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536, 65536 ], "CustomIntervals_ms": [], "Height": 1200, "MicroManagerVersion": "1.4.23 20180219", "GridColumn": 0, "UUID": "3ad8801b-5699-40d6-813b-4ad840863e38", "PixelType": "GRAY16", "Width": 1200, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\Yan\\20191216-YC113-S11S12-miseq", "Frames": 1, "Comment": "", "UserName": "Nikon", "PixelSize_um": 1.1, "ROI": [ 0, 0, 1200, 1200 ], "Date": "2019-12-17", "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC", "P-DAPI-high" ], "KeepShutterOpenSlices": true, "IJType": 1, "ComputerName": "ZadorConfocal2", "Positions": 18, "PositionIndex": 17, "CameraTimeout": 20000, "TimeFirst": false }, "640 AO-Name": "AnalogIO", "Vincent-D3-Command": "Close" }, "FrameKey-0-2-1": { "COM4-StopBits": "1", "528 AO-MaxVolts": "5.0000", "XLIGHT Excitation Wheel-State": "1", "470 Shutter-Name": "AnalogIO", "Core-Shutter": "640", "555 AO-Demo": "No", "XLIGHT Dichroic-State": "2", "XYStage-StepSize-Y": "0.1000", "ZPositionUm": 163.215, "XYStage-StepSize-X": "0.1000", "PRIME-FirmwareVersion": "20.28", "PRIME-ClearCycles": "2", "520-Name": "DA Shutter", "405 Shutter-Name": "AnalogIO", "PIEZO-StepSize": "0.0050", "PRIME-ClearMode": "Pre-Exposure", "470 AO-MinVolts": "0.0000", "405 AO-SupportsTriggering": "1", "CameraChannelIndex": 0, "XYStage-StartSpeed": "500.0000", "COM9-StopBits": "2", "405 AO-Description": "NI DAC", "COM4-DelayBetweenCharsMs": "0.0000", "445 AO-Block voltage": "false", "520 AO-TriggerInputLine": "/Dev1/PFI0", "555 AO-Description": "NI DAC", "520 AO-TriggerSampleRate": "100000", "COM4-Verbose": "1", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM4-Name": "COM4", "405 Shutter-Volts": "0.0000", "Vincent-D3-Port": "COM1", "PRIME-PP 4 MIN ADU AFFECTED": "200", "470 Shutter-MinVolts": "0.0000", "PRIME-PP 3 ENABLED": "Yes", "470 AO-Description": "NI DAC", "PRIME-Description": "PVCAM API device adapter", "520 Shutter-MaxVolts": "5.0000", "520 Shutter-Volts": "0.0000", "528 AO-Sequenceable": "1", "PRIME-Binning": "1x1", "555 Shutter-MaxVolts": "5.0000", "528 Shutter-TriggerSequenceLength": "1000", "405 Shutter-Block voltage": "false", "XLIGHT Spin motor-State": "1", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "Vincent-D3-Time to open (ms)": "35.0000", "Vincent-D3-Time to close (ms)": "35.0000", "445 AO-TriggerSampleRate": "100000", "640 Shutter-IOChannel": "Dev1/ao14", "555 AO-Sequenceable": "1", "520 AO-Name": "AnalogIO", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "470 AO-Block voltage": "false", "COM1-Verbose": "1", "COM9-AnswerTimeout": "10000.0000", "528 Shutter-Name": "AnalogIO", "PRIME-Trigger Timeout (secs)": "10", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "NextFrame": 0, "470 AO-TriggerSequenceLength": "1000", "PRIME-TransposeXY": "0", "445 AO-TriggerSequenceLength": "1000", "COM1-Name": "COM1", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "COM9-DataBits": "8", "COM1-AnswerTimeout": "500.0000", "Camera": "", "TE2000-Version": "V2.50-V2.50-V2.10", "528 Shutter-Volts": "0.0000", "XLIGHT Hub-Name": "XLIGHT Hub", "PIEZO-Description": "Ludl stage driver adapter", "PositionName": "Pos17", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSampleRate": "100000", "PRIME-FanSpeedSetpoint": "High", "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "405 AO-TriggerSampleRate": "100000", "470 Shutter-Block voltage": "false", "470-State": "0", "COM3-Parity": "None", "405 AO-Volts": "0.0000", "PRIME-TransposeMirrorY": "0", "PRIME-ChipName": "GS144BSI", "470 AO-Volts": "0.0000", "PRIME-TransposeMirrorX": "0", "445 Shutter-SupportsTriggering": "0", "470 Shutter-SupportsTriggering": "0", "XLIGHT Hub-Description": "XLIGHT hub", "PRIME-PreampOffLimit": "10000", "470 AO-Sequenceable": "1", "528 Shutter-MaxVolts": "5.0000", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "Core-XYStage": "XYStage", "470 AO-TriggerSampleRate": "100000", "PRIME-Timing-PreTriggerDelayNs": "24672000.0000", "528-DA Device": "528 Shutter", "Focus-Description": "Nikon TE2000 focus adapter", "FilterBlock-State": "5", "520 AO-Demo": "No", "COM3-DelayBetweenCharsMs": "0.0000", "470 Shutter-Volts": "0.0000", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "PRIME-PP 2 THRESHOLD": "125", "PRIME-PP 4 THRESHOLD": "75", "XYStage-Acceleration": "4.0000", "470 Shutter-Demo": "No", "PRIME-Name": "PMPCIECam00", "640-DA Device": "640 Shutter", "405 AO-Block voltage": "false", "ElapsedTime-ms": 729029, "PRIME-Offset": "100", "445 AO-IOChannel": "Dev1/ao1", "640 AO-MaxVolts": "5.0000", "PositionIndex": 17, "PRIME-PP 1 ENABLED": "Yes", "COM1-BaudRate": "9600", "640-State": "1", "COM3-Verbose": "1", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "LudlController-TransmissionDelay": "0", "PRIME-FullWellCapacity": "62000", "PRIME-SMARTStreamingEnabled": "No", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "640 Shutter-Description": "NI DAC", "528-Description": "DA device that is used as a shutter", "COM4-Description": "Serial port driver (boost:asio)", "405 Shutter-Description": "NI DAC", "TE2000-Name": "TE2000", "528 Shutter-Description": "NI DAC", "COM9-Description": "Serial port driver (boost:asio)", "LudlController-Name": "LudlController", "445 Shutter-TriggerSequenceLength": "1000", "PRIME-SerialNumber": "A18B203008", "445 AO-Volts": "0.0000", "PRIME-X-dimension": "1200", "PRIME-ShutterCloseDelay": "0", "XLIGHT Disk slider-State": "2", "Position": "Default", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 AO-IOChannel": "Dev1/ao5", "520 AO-IOChannel": "Dev1/ao4", "528 AO-TriggerSequenceLength": "1000", "405 Shutter-MaxVolts": "5.0000", "470-DA Device": "470 Shutter", "PRIME-FTCapable": "No", "Core-ChannelGroup": "Channels", "555 AO-SupportsTriggering": "0", "PRIME-Y-dimension": "1200", "Frame": 0, "555 Shutter-Sequenceable": "1", "PRIME-Timing-ExposureTimeNs": "499998000.0000", "Core-ImageProcessor": "", "528-State": "0", "520 AO-Block voltage": "false", "XLIGHT Excitation Wheel-Label": "QUAD", "640 Shutter-Demo": "No", "COM3-StopBits": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "520 Shutter-IOChannel": "Dev1/ao11", "Width": 1200, "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-SupportsTriggering": "0", "640 AO-TriggerSampleRate": "100000", "520-Description": "DA device that is used as a shutter", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "405 Shutter-Sequenceable": "1", "405 Shutter-SupportsTriggering": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-TriggerSampleRate": "100000", "640 Shutter-MaxVolts": "5.0000", "555 AO-MaxVolts": "5.0000", "520 AO-TriggerSequenceLength": "1000", "COM3-BaudRate": "9600", "COM9-BaudRate": "9600", "Vincent-D3-Channel #": "Ch. #1", "PRIME-PMode": "Normal", "OpticalPath-Label": "Left(100)", "BitDepth": 16, "555 AO-TriggerSequenceLength": "1000", "445 Shutter-Description": "NI DAC", "405 AO-Sequenceable": "1", "PRIME-AcquisitionMethod": "Callbacks", "555 AO-Name": "AnalogIO", "PRIME-ShutterMode": "Pre-Exposure", "COM3-Handshaking": "Off", "445 AO-SupportsTriggering": "0", "ROI": "0-0-1200-1200", "COM9-Verbose": "1", "PRIME-Color": "OFF", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "PRIME-ShutterOpenDelay": "0", "COM1-Parity": "None", "Slice": 1, "SlicePosition": -40, "COM9-DelayBetweenCharsMs": "0.0000", "555-Name": "DA Shutter", "PIEZO-LudlSingleAxisName": "B", "XLIGHT Emission Wheel-Label": "679/29 (A)", "640 Shutter-Name": "AnalogIO", "640 AO-Sequenceable": "1", "520 Shutter-Demo": "No", "528 Shutter-Demo": "No", "470-Name": "DA Shutter", "520-DA Device": "520 Shutter", "XYStage-Description": "Ludl XY stage driver adapter", "445 Shutter-MinVolts": "0.0000", "528 AO-TriggerSampleRate": "100000", "405 AO-MinVolts": "0.0000", "405 Shutter-TriggerSequenceLength": "1000", "Height": 1200, "555 AO-Block voltage": "false", "640 Shutter-Volts": "5.0000", "PixelType": "GRAY16", "445 Shutter-Block voltage": "false", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "520 Shutter-TriggerSequenceLength": "1000", "COM9-Name": "COM9", "Core-SLM": "", "555 AO-Volts": "0.0000", "555 Shutter-Block voltage": "false", "XYStage-Speed": "1800.0000", "520 AO-MaxVolts": "5.0000", "528 Shutter-SupportsTriggering": "0", "COM1-Description": "Serial port driver (boost:asio)", "PRIME-MetadataEnabled": "No", "520 Shutter-MinVolts": "0.0000", "470 Shutter-Sequenceable": "1", "PRIME-PreampDelay": "0", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-MinVolts": "0.0000", "ChannelIndex": 2, "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Core-AutoShutter": "1", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "Exposure-ms": 320, "PRIME-PP 2 ENABLED": "Yes", "640 Shutter-TriggerSampleRate": "100000", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "470 Shutter-IOChannel": "Dev1/ao10", "405 AO-Demo": "No", "PixelSizeUm": 1.1, "470 AO-Demo": "No", "470 Shutter-MaxVolts": "5.0000", "Vincent-D3-Name": "Vincent-D3", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FileName": "img_000000000_Il-A_001.tif", "LudlController-Reset": "Operate", "PRIME-Exposure": "320.00", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "445-Name": "DA Shutter", "555 Shutter-Name": "AnalogIO", "520 Shutter-TriggerSampleRate": "100000", "445 Shutter-IOChannel": "Dev1/ao9", "PRIME-TransposeCorrection": "0", "XYStage-StepSize": "0.1000", "445-Description": "DA device that is used as a shutter", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 AO-IOChannel": "Dev1/ao7", "520 Shutter-Name": "AnalogIO", "COM3-DataBits": "8", "640 AO-Volts": "4.5000", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "555 Shutter-IOChannel": "Dev1/ao13", "WaitInterval": null, "520 Shutter-Sequenceable": "1", "YPositionUm": -13012.300000000001, "445-State": "0", "445-DA Device": "445 Shutter", "PRIME-CircularBufferAutoSize": "ON", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "555 AO-TriggerSampleRate": "100000", "445 Shutter-Volts": "0.0000", "XLIGHT Hub-Port": "COM3", "Core-AutoFocus": "", "COM4-Handshaking": "Off", "528 Shutter-MinVolts": "0.0000", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "445 Shutter-Name": "AnalogIO", "528 Shutter-Sequenceable": "1", "XYStage-TransposeMirrorX": "0", "XYStage-TransposeMirrorY": "0", "PRIME-ReadoutRate": "100MHz 16bit", "XYStage-Name": "Stage", "640 AO-SupportsTriggering": "0", "640-Name": "DA Shutter", "640 Shutter-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "640 AO-Block voltage": "false", "445 Shutter-Sequenceable": "1", "470 AO-MaxVolts": "5.0000", "COM4-BaudRate": "9600", "470-Description": "DA device that is used as a shutter", "520 Shutter-Description": "NI DAC", "PRIME-CCDTemperature": "-14.9200", "Time": "2019-12-17 03:59:46 -0500", "640 AO-TriggerSequenceLength": "1000", "470 AO-Name": "AnalogIO", "XLIGHT Disk slider-Label": "Disk Pos 40um", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "Binning": "1x1", "TE2000-ModelType": "1", "COM3-AnswerTimeout": "500.0000", "555 Shutter-SupportsTriggering": "0", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "TE2000-Port": "COM4", "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Description": "NI DAC", "COM4-AnswerTimeout": "500.0000", "COM1-DelayBetweenCharsMs": "0.0000", "FilterBlock-Label": "Confocal", "445 AO-Name": "AnalogIO", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-Timing-ReadoutTimeNs": "24672000.0000", "528 AO-Demo": "No", "520 AO-Sequenceable": "1", "555-Description": "DA device that is used as a shutter", "405-DA Device": "405 Shutter", "405 AO-TriggerSequenceLength": "1000", "640 AO-MinVolts": "0.0000", "640 Shutter-Block voltage": "false", "FrameIndex": 0, "PRIME-Port": "CMOS", "555 AO-MinVolts": "0.0000", "COM3-Description": "Serial port driver (boost:asio)", "528 AO-Description": "NI DAC", "555 AO-IOChannel": "Dev1/ao6", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "555 AO-TriggerInputLine": "/Dev1/PFI0", "PRIME-ActualInterval-ms": "84.6200", "LudlController-Port": "COM9", "445 Shutter-Demo": "No", "PRIME-Trigger-Expose Out-Mux": "1", "SliceIndex": 1, "PRIME-CCDTemperatureSetPoint": "-15.0000", "405 Shutter-MinVolts": "0.0000", "PRIME-ExposeOutMode": "First Row", "AxisPositions": null, "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Volts": "0.0000", "528-Name": "DA Shutter", "555-State": "0", "XLIGHT Dichroic-Label": "Di03-R635-t1", "445 AO-Sequenceable": "1", "405-Name": "DA Shutter", "405 AO-IOChannel": "Dev1/ao0", "Core-Camera": "PRIME", "Focus-Name": "Focus", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "405 Shutter-IOChannel": "Dev1/ao8", "640 AO-Demo": "No", "640 Shutter-SupportsTriggering": "0", "445 AO-Demo": "No", "555-DA Device": "555 Shutter", "470 Shutter-Description": "NI DAC", "528 AO-Volts": "0.0000", "COM1-DataBits": "8", "Vincent-D3-Address": "x", "520 AO-SupportsTriggering": "0", "405-Description": "DA device that is used as a shutter", "640 Shutter-MinVolts": "0.0000", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "405 AO-Name": "AnalogIO", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "640 AO-TriggerInputLine": "/Dev1/PFI0", "405 AO-TriggerInputLine": "/Dev1/PFI0", "640-Description": "DA device that is used as a shutter", "445 Shutter-TriggerSampleRate": "100000", "470 Shutter-TriggerSequenceLength": "1000", "COM1-StopBits": "1", "520 Shutter-SupportsTriggering": "0", "405-State": "0", "Core-Initialize": "1", "XLIGHT Emission Wheel-State": "3", "520 AO-Description": "NI DAC", "528 AO-TriggerInputLine": "/Dev1/PFI0", "445 Shutter-MaxVolts": "5.0000", "COM9-Handshaking": "Off", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "XLIGHT Spin motor-Label": "On (spinning)", "PRIME-PP 5 ENABLED": "No", "UUID": "33c5a908-4187-4fd5-a4dd-3f0a229ba390", "PRIME-TriggerMode": "Internal Trigger", "COM1-Handshaking": "Off", "PRIME-Timing-PostTriggerDelayNs": "49344000.0000", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "Focus-Position": "16732.9000", "528 Shutter-Block voltage": "false", "Channel": "Il-A", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "555 Shutter-Demo": "No", "405 Shutter-Demo": "No", "528 AO-Name": "AnalogIO", "520-State": "0", "Core-Galvo": "", "405 Shutter-TriggerSampleRate": "100000", "Focus-FocusStepSize_(nm)": "100", "PRIME-PixelType": "16bit", "COM4-DataBits": "8", "470 AO-IOChannel": "Dev1/ao2", "520 AO-MinVolts": "0.0000", "COM9-Parity": "None", "PRIME-CircularBufferEnabled": "ON", "PRIME-PP 6 Reset": "No", "640 AO-Description": "NI DAC", "Source": "PRIME", "445 AO-MinVolts": "0.0000", "PIEZO-Name": "Stage", "445 AO-Description": "NI DAC", "PRIME-CircularBufferFrameCount": "46", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "520 AO-Volts": "0.0000", "528 Shutter-TriggerSampleRate": "100000", "XPositionUm": 23269.100000000002, "OpticalPath-State": "4", "PRIME-PP 3 THRESHOLD": "80", "PRIME-PVCAM Version": "3.7.1", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Gain": "1-HDR", "640 Shutter-TriggerSequenceLength": "1000", "528 AO-SupportsTriggering": "0", "445 AO-TriggerInputLine": "/Dev1/PFI0", "OpticalPath-Name": "OpticalPath", "PRIME-PP 4 ENABLED": "Yes", "PRIME-PVCAM Adapter Version": "1.3.29", "COM3-Name": "COM3", "405 AO-MaxVolts": "5.0000", "520 Shutter-Block voltage": "false", "PRIME-Timing-ClearingTimeNs": "49344000.0000", "Core-Focus": "PIEZO", "528 AO-Block voltage": "false", "TE2000-Description": "Nikon TE2000 hub", "528 AO-MinVolts": "0.0000", "COM4-Parity": "None", "Summary": { "Slices": 11, "ChContrastMin": [ 0, 0, 0, 0, 0, 0 ], "PixelAspect": 1, "SlicesFirst": true, "Interval_ms": 0, "Time": "2019-12-17 03:59:33 -0500", "Prefix": "seq_7", "InitialPositionList": [ { "Label": "Pos0", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [149.91], "Focus": [16657.7], "XYStage": [ 71629.7, 19152.9 ] }, "GridColumnIndex": 0 }, { "Label": "Pos1", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [190.82], "Focus": [16485.4], "XYStage": [ 65702.3, 19396.600000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos2", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [194.69], "Focus": [16446.8], "XYStage": [ 59880.5, 19007.8 ] }, "GridColumnIndex": 0 }, { "Label": "Pos3", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [145.04], "Focus": [16446.6], "XYStage": [ 53616.9, 18818.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos4", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [169.57500000000002], "Focus": [16446.5], "XYStage": [ 47662.8, 18817.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos5", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [131.805], "Focus": [16446.4], "XYStage": [ 41918.8, 18298.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos6", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [113.145], "Focus": [16446.5], "XYStage": [ 36129.9, 18895.8 ] }, "GridColumnIndex": 0 }, { "Label": "Pos7", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [141.005], "Focus": [16446.6], "XYStage": [ 30299.100000000002, 18895.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos8", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [140.97], "Focus": [16446.5], "XYStage": [ 23952.100000000002, 19156 ] }, "GridColumnIndex": 0 }, { "Label": "Pos9", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [184.57500000000002], "Focus": [16677.9], "XYStage": [ 72866.8, -14809.300000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos10", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [167.95000000000002], "Focus": [16557.9], "XYStage": [ 66515.6, -14371.300000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos11", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [168], "Focus": [16618.9], "XYStage": [ 59724.3, -13964.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos12", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [203.205], "Focus": [16779.6], "XYStage": [ 53862.3, -13494.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos13", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [203.20000000000002], "Focus": [16749], "XYStage": [ 48108.3, -13805.400000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos14", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [203.19], "Focus": [16675.9], "XYStage": [ 41645, -13804.900000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos15", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [203.21], "Focus": [16675.7], "XYStage": [ 35352.6, -13805.6 ] }, "GridColumnIndex": 0 }, { "Label": "Pos16", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [203.225], "Focus": [16675.8], "XYStage": [ 29298, -13447.400000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos17", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [203.215], "Focus": [16675.8], "XYStage": [ 23269.100000000002, -13012.300000000001 ] }, "GridColumnIndex": 0 } ], "z-step_um": 10, "GridRow": 0, "BitDepth": 16, "Depth": 2, "Channels": 6, "Source": "Micro-Manager", "ChColors": [ -16711681, -256, -65281, -1, -1, -16776961 ], "MetadataVersion": 10, "KeepShutterOpenChannels": true, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536, 65536 ], "CustomIntervals_ms": [], "Height": 1200, "MicroManagerVersion": "1.4.23 20180219", "GridColumn": 0, "UUID": "3ad8801b-5699-40d6-813b-4ad840863e38", "PixelType": "GRAY16", "Width": 1200, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\Yan\\20191216-YC113-S11S12-miseq", "Frames": 1, "Comment": "", "UserName": "Nikon", "PixelSize_um": 1.1, "ROI": [ 0, 0, 1200, 1200 ], "Date": "2019-12-17", "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC", "P-DAPI-high" ], "KeepShutterOpenSlices": true, "IJType": 1, "ComputerName": "ZadorConfocal2", "Positions": 18, "PositionIndex": 17, "CameraTimeout": 20000, "TimeFirst": false }, "640 AO-Name": "AnalogIO", "Vincent-D3-Command": "Close" }, "FrameKey-0-2-2": { "COM4-StopBits": "1", "528 AO-MaxVolts": "5.0000", "XLIGHT Excitation Wheel-State": "1", "470 Shutter-Name": "AnalogIO", "Core-Shutter": "640", "555 AO-Demo": "No", "XLIGHT Dichroic-State": "2", "XYStage-StepSize-Y": "0.1000", "ZPositionUm": 173.215, "XYStage-StepSize-X": "0.1000", "PRIME-FirmwareVersion": "20.28", "PRIME-ClearCycles": "2", "520-Name": "DA Shutter", "405 Shutter-Name": "AnalogIO", "PIEZO-StepSize": "0.0050", "PRIME-ClearMode": "Pre-Exposure", "470 AO-MinVolts": "0.0000", "405 AO-SupportsTriggering": "1", "CameraChannelIndex": 0, "XYStage-StartSpeed": "500.0000", "COM9-StopBits": "2", "405 AO-Description": "NI DAC", "COM4-DelayBetweenCharsMs": "0.0000", "445 AO-Block voltage": "false", "520 AO-TriggerInputLine": "/Dev1/PFI0", "555 AO-Description": "NI DAC", "520 AO-TriggerSampleRate": "100000", "COM4-Verbose": "1", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM4-Name": "COM4", "405 Shutter-Volts": "0.0000", "Vincent-D3-Port": "COM1", "PRIME-PP 4 MIN ADU AFFECTED": "200", "470 Shutter-MinVolts": "0.0000", "PRIME-PP 3 ENABLED": "Yes", "470 AO-Description": "NI DAC", "PRIME-Description": "PVCAM API device adapter", "520 Shutter-MaxVolts": "5.0000", "520 Shutter-Volts": "0.0000", "528 AO-Sequenceable": "1", "PRIME-Binning": "1x1", "555 Shutter-MaxVolts": "5.0000", "528 Shutter-TriggerSequenceLength": "1000", "405 Shutter-Block voltage": "false", "XLIGHT Spin motor-State": "1", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "Vincent-D3-Time to open (ms)": "35.0000", "Vincent-D3-Time to close (ms)": "35.0000", "445 AO-TriggerSampleRate": "100000", "640 Shutter-IOChannel": "Dev1/ao14", "555 AO-Sequenceable": "1", "520 AO-Name": "AnalogIO", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "470 AO-Block voltage": "false", "COM1-Verbose": "1", "COM9-AnswerTimeout": "10000.0000", "528 Shutter-Name": "AnalogIO", "PRIME-Trigger Timeout (secs)": "10", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "NextFrame": 0, "470 AO-TriggerSequenceLength": "1000", "PRIME-TransposeXY": "0", "445 AO-TriggerSequenceLength": "1000", "COM1-Name": "COM1", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "COM9-DataBits": "8", "COM1-AnswerTimeout": "500.0000", "Camera": "", "TE2000-Version": "V2.50-V2.50-V2.10", "528 Shutter-Volts": "0.0000", "XLIGHT Hub-Name": "XLIGHT Hub", "PIEZO-Description": "Ludl stage driver adapter", "PositionName": "Pos17", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSampleRate": "100000", "PRIME-FanSpeedSetpoint": "High", "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "405 AO-TriggerSampleRate": "100000", "470 Shutter-Block voltage": "false", "470-State": "0", "COM3-Parity": "None", "405 AO-Volts": "0.0000", "PRIME-TransposeMirrorY": "0", "PRIME-ChipName": "GS144BSI", "470 AO-Volts": "0.0000", "PRIME-TransposeMirrorX": "0", "445 Shutter-SupportsTriggering": "0", "470 Shutter-SupportsTriggering": "0", "XLIGHT Hub-Description": "XLIGHT hub", "PRIME-PreampOffLimit": "10000", "470 AO-Sequenceable": "1", "528 Shutter-MaxVolts": "5.0000", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "Core-XYStage": "XYStage", "470 AO-TriggerSampleRate": "100000", "PRIME-Timing-PreTriggerDelayNs": "24672000.0000", "528-DA Device": "528 Shutter", "Focus-Description": "Nikon TE2000 focus adapter", "FilterBlock-State": "5", "520 AO-Demo": "No", "COM3-DelayBetweenCharsMs": "0.0000", "470 Shutter-Volts": "0.0000", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "PRIME-PP 2 THRESHOLD": "125", "PRIME-PP 4 THRESHOLD": "75", "XYStage-Acceleration": "4.0000", "470 Shutter-Demo": "No", "PRIME-Name": "PMPCIECam00", "640-DA Device": "640 Shutter", "405 AO-Block voltage": "false", "ElapsedTime-ms": 729640, "PRIME-Offset": "100", "445 AO-IOChannel": "Dev1/ao1", "640 AO-MaxVolts": "5.0000", "PositionIndex": 17, "PRIME-PP 1 ENABLED": "Yes", "COM1-BaudRate": "9600", "640-State": "1", "COM3-Verbose": "1", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "LudlController-TransmissionDelay": "0", "PRIME-FullWellCapacity": "62000", "PRIME-SMARTStreamingEnabled": "No", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "640 Shutter-Description": "NI DAC", "528-Description": "DA device that is used as a shutter", "COM4-Description": "Serial port driver (boost:asio)", "405 Shutter-Description": "NI DAC", "TE2000-Name": "TE2000", "528 Shutter-Description": "NI DAC", "COM9-Description": "Serial port driver (boost:asio)", "LudlController-Name": "LudlController", "445 Shutter-TriggerSequenceLength": "1000", "PRIME-SerialNumber": "A18B203008", "445 AO-Volts": "0.0000", "PRIME-X-dimension": "1200", "PRIME-ShutterCloseDelay": "0", "XLIGHT Disk slider-State": "2", "Position": "Default", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 AO-IOChannel": "Dev1/ao5", "520 AO-IOChannel": "Dev1/ao4", "528 AO-TriggerSequenceLength": "1000", "405 Shutter-MaxVolts": "5.0000", "470-DA Device": "470 Shutter", "PRIME-FTCapable": "No", "Core-ChannelGroup": "Channels", "555 AO-SupportsTriggering": "0", "PRIME-Y-dimension": "1200", "Frame": 0, "555 Shutter-Sequenceable": "1", "PRIME-Timing-ExposureTimeNs": "499998000.0000", "Core-ImageProcessor": "", "528-State": "0", "520 AO-Block voltage": "false", "XLIGHT Excitation Wheel-Label": "QUAD", "640 Shutter-Demo": "No", "COM3-StopBits": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "520 Shutter-IOChannel": "Dev1/ao11", "Width": 1200, "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-SupportsTriggering": "0", "640 AO-TriggerSampleRate": "100000", "520-Description": "DA device that is used as a shutter", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "405 Shutter-Sequenceable": "1", "405 Shutter-SupportsTriggering": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-TriggerSampleRate": "100000", "640 Shutter-MaxVolts": "5.0000", "555 AO-MaxVolts": "5.0000", "520 AO-TriggerSequenceLength": "1000", "COM3-BaudRate": "9600", "COM9-BaudRate": "9600", "Vincent-D3-Channel #": "Ch. #1", "PRIME-PMode": "Normal", "OpticalPath-Label": "Left(100)", "BitDepth": 16, "555 AO-TriggerSequenceLength": "1000", "445 Shutter-Description": "NI DAC", "405 AO-Sequenceable": "1", "PRIME-AcquisitionMethod": "Callbacks", "555 AO-Name": "AnalogIO", "PRIME-ShutterMode": "Pre-Exposure", "COM3-Handshaking": "Off", "445 AO-SupportsTriggering": "0", "ROI": "0-0-1200-1200", "COM9-Verbose": "1", "PRIME-Color": "OFF", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "PRIME-ShutterOpenDelay": "0", "COM1-Parity": "None", "Slice": 2, "SlicePosition": -30, "COM9-DelayBetweenCharsMs": "0.0000", "555-Name": "DA Shutter", "PIEZO-LudlSingleAxisName": "B", "XLIGHT Emission Wheel-Label": "679/29 (A)", "640 Shutter-Name": "AnalogIO", "640 AO-Sequenceable": "1", "520 Shutter-Demo": "No", "528 Shutter-Demo": "No", "470-Name": "DA Shutter", "520-DA Device": "520 Shutter", "XYStage-Description": "Ludl XY stage driver adapter", "445 Shutter-MinVolts": "0.0000", "528 AO-TriggerSampleRate": "100000", "405 AO-MinVolts": "0.0000", "405 Shutter-TriggerSequenceLength": "1000", "Height": 1200, "555 AO-Block voltage": "false", "640 Shutter-Volts": "5.0000", "PixelType": "GRAY16", "445 Shutter-Block voltage": "false", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "520 Shutter-TriggerSequenceLength": "1000", "COM9-Name": "COM9", "Core-SLM": "", "555 AO-Volts": "0.0000", "555 Shutter-Block voltage": "false", "XYStage-Speed": "1800.0000", "520 AO-MaxVolts": "5.0000", "528 Shutter-SupportsTriggering": "0", "COM1-Description": "Serial port driver (boost:asio)", "PRIME-MetadataEnabled": "No", "520 Shutter-MinVolts": "0.0000", "470 Shutter-Sequenceable": "1", "PRIME-PreampDelay": "0", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-MinVolts": "0.0000", "ChannelIndex": 2, "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Core-AutoShutter": "1", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "Exposure-ms": 320, "PRIME-PP 2 ENABLED": "Yes", "640 Shutter-TriggerSampleRate": "100000", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "470 Shutter-IOChannel": "Dev1/ao10", "405 AO-Demo": "No", "PixelSizeUm": 1.1, "470 AO-Demo": "No", "470 Shutter-MaxVolts": "5.0000", "Vincent-D3-Name": "Vincent-D3", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FileName": "img_000000000_Il-A_002.tif", "LudlController-Reset": "Operate", "PRIME-Exposure": "320.00", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "445-Name": "DA Shutter", "555 Shutter-Name": "AnalogIO", "520 Shutter-TriggerSampleRate": "100000", "445 Shutter-IOChannel": "Dev1/ao9", "PRIME-TransposeCorrection": "0", "XYStage-StepSize": "0.1000", "445-Description": "DA device that is used as a shutter", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 AO-IOChannel": "Dev1/ao7", "520 Shutter-Name": "AnalogIO", "COM3-DataBits": "8", "640 AO-Volts": "4.5000", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "555 Shutter-IOChannel": "Dev1/ao13", "WaitInterval": null, "520 Shutter-Sequenceable": "1", "YPositionUm": -13012.300000000001, "445-State": "0", "445-DA Device": "445 Shutter", "PRIME-CircularBufferAutoSize": "ON", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "555 AO-TriggerSampleRate": "100000", "445 Shutter-Volts": "0.0000", "XLIGHT Hub-Port": "COM3", "Core-AutoFocus": "", "COM4-Handshaking": "Off", "528 Shutter-MinVolts": "0.0000", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "445 Shutter-Name": "AnalogIO", "528 Shutter-Sequenceable": "1", "XYStage-TransposeMirrorX": "0", "XYStage-TransposeMirrorY": "0", "PRIME-ReadoutRate": "100MHz 16bit", "XYStage-Name": "Stage", "640 AO-SupportsTriggering": "0", "640-Name": "DA Shutter", "640 Shutter-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "640 AO-Block voltage": "false", "445 Shutter-Sequenceable": "1", "470 AO-MaxVolts": "5.0000", "COM4-BaudRate": "9600", "470-Description": "DA device that is used as a shutter", "520 Shutter-Description": "NI DAC", "PRIME-CCDTemperature": "-14.9200", "Time": "2019-12-17 03:59:46 -0500", "640 AO-TriggerSequenceLength": "1000", "470 AO-Name": "AnalogIO", "XLIGHT Disk slider-Label": "Disk Pos 40um", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "Binning": "1x1", "TE2000-ModelType": "1", "COM3-AnswerTimeout": "500.0000", "555 Shutter-SupportsTriggering": "0", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "TE2000-Port": "COM4", "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Description": "NI DAC", "COM4-AnswerTimeout": "500.0000", "COM1-DelayBetweenCharsMs": "0.0000", "FilterBlock-Label": "Confocal", "445 AO-Name": "AnalogIO", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-Timing-ReadoutTimeNs": "24672000.0000", "528 AO-Demo": "No", "520 AO-Sequenceable": "1", "555-Description": "DA device that is used as a shutter", "405-DA Device": "405 Shutter", "405 AO-TriggerSequenceLength": "1000", "640 AO-MinVolts": "0.0000", "640 Shutter-Block voltage": "false", "FrameIndex": 0, "PRIME-Port": "CMOS", "555 AO-MinVolts": "0.0000", "COM3-Description": "Serial port driver (boost:asio)", "528 AO-Description": "NI DAC", "555 AO-IOChannel": "Dev1/ao6", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "555 AO-TriggerInputLine": "/Dev1/PFI0", "PRIME-ActualInterval-ms": "84.6200", "LudlController-Port": "COM9", "445 Shutter-Demo": "No", "PRIME-Trigger-Expose Out-Mux": "1", "SliceIndex": 2, "PRIME-CCDTemperatureSetPoint": "-15.0000", "405 Shutter-MinVolts": "0.0000", "PRIME-ExposeOutMode": "First Row", "AxisPositions": null, "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Volts": "0.0000", "528-Name": "DA Shutter", "555-State": "0", "XLIGHT Dichroic-Label": "Di03-R635-t1", "445 AO-Sequenceable": "1", "405-Name": "DA Shutter", "405 AO-IOChannel": "Dev1/ao0", "Core-Camera": "PRIME", "Focus-Name": "Focus", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "405 Shutter-IOChannel": "Dev1/ao8", "640 AO-Demo": "No", "640 Shutter-SupportsTriggering": "0", "445 AO-Demo": "No", "555-DA Device": "555 Shutter", "470 Shutter-Description": "NI DAC", "528 AO-Volts": "0.0000", "COM1-DataBits": "8", "Vincent-D3-Address": "x", "520 AO-SupportsTriggering": "0", "405-Description": "DA device that is used as a shutter", "640 Shutter-MinVolts": "0.0000", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "405 AO-Name": "AnalogIO", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "640 AO-TriggerInputLine": "/Dev1/PFI0", "405 AO-TriggerInputLine": "/Dev1/PFI0", "640-Description": "DA device that is used as a shutter", "445 Shutter-TriggerSampleRate": "100000", "470 Shutter-TriggerSequenceLength": "1000", "COM1-StopBits": "1", "520 Shutter-SupportsTriggering": "0", "405-State": "0", "Core-Initialize": "1", "XLIGHT Emission Wheel-State": "3", "520 AO-Description": "NI DAC", "528 AO-TriggerInputLine": "/Dev1/PFI0", "445 Shutter-MaxVolts": "5.0000", "COM9-Handshaking": "Off", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "XLIGHT Spin motor-Label": "On (spinning)", "PRIME-PP 5 ENABLED": "No", "UUID": "42df0c12-ff45-4aa5-95b4-8e6bcc2b0cac", "PRIME-TriggerMode": "Internal Trigger", "COM1-Handshaking": "Off", "PRIME-Timing-PostTriggerDelayNs": "49344000.0000", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "Focus-Position": "16732.9000", "528 Shutter-Block voltage": "false", "Channel": "Il-A", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "555 Shutter-Demo": "No", "405 Shutter-Demo": "No", "528 AO-Name": "AnalogIO", "520-State": "0", "Core-Galvo": "", "405 Shutter-TriggerSampleRate": "100000", "Focus-FocusStepSize_(nm)": "100", "PRIME-PixelType": "16bit", "COM4-DataBits": "8", "470 AO-IOChannel": "Dev1/ao2", "520 AO-MinVolts": "0.0000", "COM9-Parity": "None", "PRIME-CircularBufferEnabled": "ON", "PRIME-PP 6 Reset": "No", "640 AO-Description": "NI DAC", "Source": "PRIME", "445 AO-MinVolts": "0.0000", "PIEZO-Name": "Stage", "445 AO-Description": "NI DAC", "PRIME-CircularBufferFrameCount": "46", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "520 AO-Volts": "0.0000", "528 Shutter-TriggerSampleRate": "100000", "XPositionUm": 23269.100000000002, "OpticalPath-State": "4", "PRIME-PP 3 THRESHOLD": "80", "PRIME-PVCAM Version": "3.7.1", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Gain": "1-HDR", "640 Shutter-TriggerSequenceLength": "1000", "528 AO-SupportsTriggering": "0", "445 AO-TriggerInputLine": "/Dev1/PFI0", "OpticalPath-Name": "OpticalPath", "PRIME-PP 4 ENABLED": "Yes", "PRIME-PVCAM Adapter Version": "1.3.29", "COM3-Name": "COM3", "405 AO-MaxVolts": "5.0000", "520 Shutter-Block voltage": "false", "PRIME-Timing-ClearingTimeNs": "49344000.0000", "Core-Focus": "PIEZO", "528 AO-Block voltage": "false", "TE2000-Description": "Nikon TE2000 hub", "528 AO-MinVolts": "0.0000", "COM4-Parity": "None", "Summary": { "Slices": 11, "ChContrastMin": [ 0, 0, 0, 0, 0, 0 ], "PixelAspect": 1, "SlicesFirst": true, "Interval_ms": 0, "Time": "2019-12-17 03:59:33 -0500", "Prefix": "seq_7", "InitialPositionList": [ { "Label": "Pos0", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [149.91], "Focus": [16657.7], "XYStage": [ 71629.7, 19152.9 ] }, "GridColumnIndex": 0 }, { "Label": "Pos1", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [190.82], "Focus": [16485.4], "XYStage": [ 65702.3, 19396.600000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos2", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [194.69], "Focus": [16446.8], "XYStage": [ 59880.5, 19007.8 ] }, "GridColumnIndex": 0 }, { "Label": "Pos3", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [145.04], "Focus": [16446.6], "XYStage": [ 53616.9, 18818.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos4", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [169.57500000000002], "Focus": [16446.5], "XYStage": [ 47662.8, 18817.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos5", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [131.805], "Focus": [16446.4], "XYStage": [ 41918.8, 18298.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos6", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [113.145], "Focus": [16446.5], "XYStage": [ 36129.9, 18895.8 ] }, "GridColumnIndex": 0 }, { "Label": "Pos7", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [141.005], "Focus": [16446.6], "XYStage": [ 30299.100000000002, 18895.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos8", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [140.97], "Focus": [16446.5], "XYStage": [ 23952.100000000002, 19156 ] }, "GridColumnIndex": 0 }, { "Label": "Pos9", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [184.57500000000002], "Focus": [16677.9], "XYStage": [ 72866.8, -14809.300000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos10", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [167.95000000000002], "Focus": [16557.9], "XYStage": [ 66515.6, -14371.300000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos11", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [168], "Focus": [16618.9], "XYStage": [ 59724.3, -13964.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos12", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [203.205], "Focus": [16779.6], "XYStage": [ 53862.3, -13494.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos13", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [203.20000000000002], "Focus": [16749], "XYStage": [ 48108.3, -13805.400000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos14", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [203.19], "Focus": [16675.9], "XYStage": [ 41645, -13804.900000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos15", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [203.21], "Focus": [16675.7], "XYStage": [ 35352.6, -13805.6 ] }, "GridColumnIndex": 0 }, { "Label": "Pos16", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [203.225], "Focus": [16675.8], "XYStage": [ 29298, -13447.400000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos17", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [203.215], "Focus": [16675.8], "XYStage": [ 23269.100000000002, -13012.300000000001 ] }, "GridColumnIndex": 0 } ], "z-step_um": 10, "GridRow": 0, "BitDepth": 16, "Depth": 2, "Channels": 6, "Source": "Micro-Manager", "ChColors": [ -16711681, -256, -65281, -1, -1, -16776961 ], "MetadataVersion": 10, "KeepShutterOpenChannels": true, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536, 65536 ], "CustomIntervals_ms": [], "Height": 1200, "MicroManagerVersion": "1.4.23 20180219", "GridColumn": 0, "UUID": "3ad8801b-5699-40d6-813b-4ad840863e38", "PixelType": "GRAY16", "Width": 1200, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\Yan\\20191216-YC113-S11S12-miseq", "Frames": 1, "Comment": "", "UserName": "Nikon", "PixelSize_um": 1.1, "ROI": [ 0, 0, 1200, 1200 ], "Date": "2019-12-17", "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC", "P-DAPI-high" ], "KeepShutterOpenSlices": true, "IJType": 1, "ComputerName": "ZadorConfocal2", "Positions": 18, "PositionIndex": 17, "CameraTimeout": 20000, "TimeFirst": false }, "640 AO-Name": "AnalogIO", "Vincent-D3-Command": "Close" }, "FrameKey-0-2-3": { "COM4-StopBits": "1", "528 AO-MaxVolts": "5.0000", "XLIGHT Excitation Wheel-State": "1", "470 Shutter-Name": "AnalogIO", "Core-Shutter": "640", "555 AO-Demo": "No", "XLIGHT Dichroic-State": "2", "XYStage-StepSize-Y": "0.1000", "ZPositionUm": 183.215, "XYStage-StepSize-X": "0.1000", "PRIME-FirmwareVersion": "20.28", "PRIME-ClearCycles": "2", "520-Name": "DA Shutter", "405 Shutter-Name": "AnalogIO", "PIEZO-StepSize": "0.0050", "PRIME-ClearMode": "Pre-Exposure", "470 AO-MinVolts": "0.0000", "405 AO-SupportsTriggering": "1", "CameraChannelIndex": 0, "XYStage-StartSpeed": "500.0000", "COM9-StopBits": "2", "405 AO-Description": "NI DAC", "COM4-DelayBetweenCharsMs": "0.0000", "445 AO-Block voltage": "false", "520 AO-TriggerInputLine": "/Dev1/PFI0", "555 AO-Description": "NI DAC", "520 AO-TriggerSampleRate": "100000", "COM4-Verbose": "1", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM4-Name": "COM4", "405 Shutter-Volts": "0.0000", "Vincent-D3-Port": "COM1", "PRIME-PP 4 MIN ADU AFFECTED": "200", "470 Shutter-MinVolts": "0.0000", "PRIME-PP 3 ENABLED": "Yes", "470 AO-Description": "NI DAC", "PRIME-Description": "PVCAM API device adapter", "520 Shutter-MaxVolts": "5.0000", "520 Shutter-Volts": "0.0000", "528 AO-Sequenceable": "1", "PRIME-Binning": "1x1", "555 Shutter-MaxVolts": "5.0000", "528 Shutter-TriggerSequenceLength": "1000", "405 Shutter-Block voltage": "false", "XLIGHT Spin motor-State": "1", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "Vincent-D3-Time to open (ms)": "35.0000", "Vincent-D3-Time to close (ms)": "35.0000", "445 AO-TriggerSampleRate": "100000", "640 Shutter-IOChannel": "Dev1/ao14", "555 AO-Sequenceable": "1", "520 AO-Name": "AnalogIO", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "470 AO-Block voltage": "false", "COM1-Verbose": "1", "COM9-AnswerTimeout": "10000.0000", "528 Shutter-Name": "AnalogIO", "PRIME-Trigger Timeout (secs)": "10", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "NextFrame": 0, "470 AO-TriggerSequenceLength": "1000", "PRIME-TransposeXY": "0", "445 AO-TriggerSequenceLength": "1000", "COM1-Name": "COM1", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "COM9-DataBits": "8", "COM1-AnswerTimeout": "500.0000", "Camera": "", "TE2000-Version": "V2.50-V2.50-V2.10", "528 Shutter-Volts": "0.0000", "XLIGHT Hub-Name": "XLIGHT Hub", "PIEZO-Description": "Ludl stage driver adapter", "PositionName": "Pos17", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSampleRate": "100000", "PRIME-FanSpeedSetpoint": "High", "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "405 AO-TriggerSampleRate": "100000", "470 Shutter-Block voltage": "false", "470-State": "0", "COM3-Parity": "None", "405 AO-Volts": "0.0000", "PRIME-TransposeMirrorY": "0", "PRIME-ChipName": "GS144BSI", "470 AO-Volts": "0.0000", "PRIME-TransposeMirrorX": "0", "445 Shutter-SupportsTriggering": "0", "470 Shutter-SupportsTriggering": "0", "XLIGHT Hub-Description": "XLIGHT hub", "PRIME-PreampOffLimit": "10000", "470 AO-Sequenceable": "1", "528 Shutter-MaxVolts": "5.0000", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "Core-XYStage": "XYStage", "470 AO-TriggerSampleRate": "100000", "PRIME-Timing-PreTriggerDelayNs": "24672000.0000", "528-DA Device": "528 Shutter", "Focus-Description": "Nikon TE2000 focus adapter", "FilterBlock-State": "5", "520 AO-Demo": "No", "COM3-DelayBetweenCharsMs": "0.0000", "470 Shutter-Volts": "0.0000", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "PRIME-PP 2 THRESHOLD": "125", "PRIME-PP 4 THRESHOLD": "75", "XYStage-Acceleration": "4.0000", "470 Shutter-Demo": "No", "PRIME-Name": "PMPCIECam00", "640-DA Device": "640 Shutter", "405 AO-Block voltage": "false", "ElapsedTime-ms": 730195, "PRIME-Offset": "100", "445 AO-IOChannel": "Dev1/ao1", "640 AO-MaxVolts": "5.0000", "PositionIndex": 17, "PRIME-PP 1 ENABLED": "Yes", "COM1-BaudRate": "9600", "640-State": "1", "COM3-Verbose": "1", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "LudlController-TransmissionDelay": "0", "PRIME-FullWellCapacity": "62000", "PRIME-SMARTStreamingEnabled": "No", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "640 Shutter-Description": "NI DAC", "528-Description": "DA device that is used as a shutter", "COM4-Description": "Serial port driver (boost:asio)", "405 Shutter-Description": "NI DAC", "TE2000-Name": "TE2000", "528 Shutter-Description": "NI DAC", "COM9-Description": "Serial port driver (boost:asio)", "LudlController-Name": "LudlController", "445 Shutter-TriggerSequenceLength": "1000", "PRIME-SerialNumber": "A18B203008", "445 AO-Volts": "0.0000", "PRIME-X-dimension": "1200", "PRIME-ShutterCloseDelay": "0", "XLIGHT Disk slider-State": "2", "Position": "Default", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 AO-IOChannel": "Dev1/ao5", "520 AO-IOChannel": "Dev1/ao4", "528 AO-TriggerSequenceLength": "1000", "405 Shutter-MaxVolts": "5.0000", "470-DA Device": "470 Shutter", "PRIME-FTCapable": "No", "Core-ChannelGroup": "Channels", "555 AO-SupportsTriggering": "0", "PRIME-Y-dimension": "1200", "Frame": 0, "555 Shutter-Sequenceable": "1", "PRIME-Timing-ExposureTimeNs": "499998000.0000", "Core-ImageProcessor": "", "528-State": "0", "520 AO-Block voltage": "false", "XLIGHT Excitation Wheel-Label": "QUAD", "640 Shutter-Demo": "No", "COM3-StopBits": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "520 Shutter-IOChannel": "Dev1/ao11", "Width": 1200, "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-SupportsTriggering": "0", "640 AO-TriggerSampleRate": "100000", "520-Description": "DA device that is used as a shutter", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "405 Shutter-Sequenceable": "1", "405 Shutter-SupportsTriggering": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-TriggerSampleRate": "100000", "640 Shutter-MaxVolts": "5.0000", "555 AO-MaxVolts": "5.0000", "520 AO-TriggerSequenceLength": "1000", "COM3-BaudRate": "9600", "COM9-BaudRate": "9600", "Vincent-D3-Channel #": "Ch. #1", "PRIME-PMode": "Normal", "OpticalPath-Label": "Left(100)", "BitDepth": 16, "555 AO-TriggerSequenceLength": "1000", "445 Shutter-Description": "NI DAC", "405 AO-Sequenceable": "1", "PRIME-AcquisitionMethod": "Callbacks", "555 AO-Name": "AnalogIO", "PRIME-ShutterMode": "Pre-Exposure", "COM3-Handshaking": "Off", "445 AO-SupportsTriggering": "0", "ROI": "0-0-1200-1200", "COM9-Verbose": "1", "PRIME-Color": "OFF", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "PRIME-ShutterOpenDelay": "0", "COM1-Parity": "None", "Slice": 3, "SlicePosition": -20, "COM9-DelayBetweenCharsMs": "0.0000", "555-Name": "DA Shutter", "PIEZO-LudlSingleAxisName": "B", "XLIGHT Emission Wheel-Label": "679/29 (A)", "640 Shutter-Name": "AnalogIO", "640 AO-Sequenceable": "1", "520 Shutter-Demo": "No", "528 Shutter-Demo": "No", "470-Name": "DA Shutter", "520-DA Device": "520 Shutter", "XYStage-Description": "Ludl XY stage driver adapter", "445 Shutter-MinVolts": "0.0000", "528 AO-TriggerSampleRate": "100000", "405 AO-MinVolts": "0.0000", "405 Shutter-TriggerSequenceLength": "1000", "Height": 1200, "555 AO-Block voltage": "false", "640 Shutter-Volts": "5.0000", "PixelType": "GRAY16", "445 Shutter-Block voltage": "false", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "520 Shutter-TriggerSequenceLength": "1000", "COM9-Name": "COM9", "Core-SLM": "", "555 AO-Volts": "0.0000", "555 Shutter-Block voltage": "false", "XYStage-Speed": "1800.0000", "520 AO-MaxVolts": "5.0000", "528 Shutter-SupportsTriggering": "0", "COM1-Description": "Serial port driver (boost:asio)", "PRIME-MetadataEnabled": "No", "520 Shutter-MinVolts": "0.0000", "470 Shutter-Sequenceable": "1", "PRIME-PreampDelay": "0", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-MinVolts": "0.0000", "ChannelIndex": 2, "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Core-AutoShutter": "1", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "Exposure-ms": 320, "PRIME-PP 2 ENABLED": "Yes", "640 Shutter-TriggerSampleRate": "100000", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "470 Shutter-IOChannel": "Dev1/ao10", "405 AO-Demo": "No", "PixelSizeUm": 1.1, "470 AO-Demo": "No", "470 Shutter-MaxVolts": "5.0000", "Vincent-D3-Name": "Vincent-D3", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FileName": "img_000000000_Il-A_003.tif", "LudlController-Reset": "Operate", "PRIME-Exposure": "320.00", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "445-Name": "DA Shutter", "555 Shutter-Name": "AnalogIO", "520 Shutter-TriggerSampleRate": "100000", "445 Shutter-IOChannel": "Dev1/ao9", "PRIME-TransposeCorrection": "0", "XYStage-StepSize": "0.1000", "445-Description": "DA device that is used as a shutter", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 AO-IOChannel": "Dev1/ao7", "520 Shutter-Name": "AnalogIO", "COM3-DataBits": "8", "640 AO-Volts": "4.5000", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "555 Shutter-IOChannel": "Dev1/ao13", "WaitInterval": null, "520 Shutter-Sequenceable": "1", "YPositionUm": -13012.300000000001, "445-State": "0", "445-DA Device": "445 Shutter", "PRIME-CircularBufferAutoSize": "ON", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "555 AO-TriggerSampleRate": "100000", "445 Shutter-Volts": "0.0000", "XLIGHT Hub-Port": "COM3", "Core-AutoFocus": "", "COM4-Handshaking": "Off", "528 Shutter-MinVolts": "0.0000", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "445 Shutter-Name": "AnalogIO", "528 Shutter-Sequenceable": "1", "XYStage-TransposeMirrorX": "0", "XYStage-TransposeMirrorY": "0", "PRIME-ReadoutRate": "100MHz 16bit", "XYStage-Name": "Stage", "640 AO-SupportsTriggering": "0", "640-Name": "DA Shutter", "640 Shutter-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "640 AO-Block voltage": "false", "445 Shutter-Sequenceable": "1", "470 AO-MaxVolts": "5.0000", "COM4-BaudRate": "9600", "470-Description": "DA device that is used as a shutter", "520 Shutter-Description": "NI DAC", "PRIME-CCDTemperature": "-14.9200", "Time": "2019-12-17 03:59:47 -0500", "640 AO-TriggerSequenceLength": "1000", "470 AO-Name": "AnalogIO", "XLIGHT Disk slider-Label": "Disk Pos 40um", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "Binning": "1x1", "TE2000-ModelType": "1", "COM3-AnswerTimeout": "500.0000", "555 Shutter-SupportsTriggering": "0", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "TE2000-Port": "COM4", "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Description": "NI DAC", "COM4-AnswerTimeout": "500.0000", "COM1-DelayBetweenCharsMs": "0.0000", "FilterBlock-Label": "Confocal", "445 AO-Name": "AnalogIO", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-Timing-ReadoutTimeNs": "24672000.0000", "528 AO-Demo": "No", "520 AO-Sequenceable": "1", "555-Description": "DA device that is used as a shutter", "405-DA Device": "405 Shutter", "405 AO-TriggerSequenceLength": "1000", "640 AO-MinVolts": "0.0000", "640 Shutter-Block voltage": "false", "FrameIndex": 0, "PRIME-Port": "CMOS", "555 AO-MinVolts": "0.0000", "COM3-Description": "Serial port driver (boost:asio)", "528 AO-Description": "NI DAC", "555 AO-IOChannel": "Dev1/ao6", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "555 AO-TriggerInputLine": "/Dev1/PFI0", "PRIME-ActualInterval-ms": "84.6200", "LudlController-Port": "COM9", "445 Shutter-Demo": "No", "PRIME-Trigger-Expose Out-Mux": "1", "SliceIndex": 3, "PRIME-CCDTemperatureSetPoint": "-15.0000", "405 Shutter-MinVolts": "0.0000", "PRIME-ExposeOutMode": "First Row", "AxisPositions": null, "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Volts": "0.0000", "528-Name": "DA Shutter", "555-State": "0", "XLIGHT Dichroic-Label": "Di03-R635-t1", "445 AO-Sequenceable": "1", "405-Name": "DA Shutter", "405 AO-IOChannel": "Dev1/ao0", "Core-Camera": "PRIME", "Focus-Name": "Focus", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "405 Shutter-IOChannel": "Dev1/ao8", "640 AO-Demo": "No", "640 Shutter-SupportsTriggering": "0", "445 AO-Demo": "No", "555-DA Device": "555 Shutter", "470 Shutter-Description": "NI DAC", "528 AO-Volts": "0.0000", "COM1-DataBits": "8", "Vincent-D3-Address": "x", "520 AO-SupportsTriggering": "0", "405-Description": "DA device that is used as a shutter", "640 Shutter-MinVolts": "0.0000", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "405 AO-Name": "AnalogIO", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "640 AO-TriggerInputLine": "/Dev1/PFI0", "405 AO-TriggerInputLine": "/Dev1/PFI0", "640-Description": "DA device that is used as a shutter", "445 Shutter-TriggerSampleRate": "100000", "470 Shutter-TriggerSequenceLength": "1000", "COM1-StopBits": "1", "520 Shutter-SupportsTriggering": "0", "405-State": "0", "Core-Initialize": "1", "XLIGHT Emission Wheel-State": "3", "520 AO-Description": "NI DAC", "528 AO-TriggerInputLine": "/Dev1/PFI0", "445 Shutter-MaxVolts": "5.0000", "COM9-Handshaking": "Off", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "XLIGHT Spin motor-Label": "On (spinning)", "PRIME-PP 5 ENABLED": "No", "UUID": "2ec55ec5-f205-4453-ad21-730305d5ecd2", "PRIME-TriggerMode": "Internal Trigger", "COM1-Handshaking": "Off", "PRIME-Timing-PostTriggerDelayNs": "49344000.0000", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "Focus-Position": "16732.9000", "528 Shutter-Block voltage": "false", "Channel": "Il-A", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "555 Shutter-Demo": "No", "405 Shutter-Demo": "No", "528 AO-Name": "AnalogIO", "520-State": "0", "Core-Galvo": "", "405 Shutter-TriggerSampleRate": "100000", "Focus-FocusStepSize_(nm)": "100", "PRIME-PixelType": "16bit", "COM4-DataBits": "8", "470 AO-IOChannel": "Dev1/ao2", "520 AO-MinVolts": "0.0000", "COM9-Parity": "None", "PRIME-CircularBufferEnabled": "ON", "PRIME-PP 6 Reset": "No", "640 AO-Description": "NI DAC", "Source": "PRIME", "445 AO-MinVolts": "0.0000", "PIEZO-Name": "Stage", "445 AO-Description": "NI DAC", "PRIME-CircularBufferFrameCount": "46", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "520 AO-Volts": "0.0000", "528 Shutter-TriggerSampleRate": "100000", "XPositionUm": 23269.100000000002, "OpticalPath-State": "4", "PRIME-PP 3 THRESHOLD": "80", "PRIME-PVCAM Version": "3.7.1", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Gain": "1-HDR", "640 Shutter-TriggerSequenceLength": "1000", "528 AO-SupportsTriggering": "0", "445 AO-TriggerInputLine": "/Dev1/PFI0", "OpticalPath-Name": "OpticalPath", "PRIME-PP 4 ENABLED": "Yes", "PRIME-PVCAM Adapter Version": "1.3.29", "COM3-Name": "COM3", "405 AO-MaxVolts": "5.0000", "520 Shutter-Block voltage": "false", "PRIME-Timing-ClearingTimeNs": "49344000.0000", "Core-Focus": "PIEZO", "528 AO-Block voltage": "false", "TE2000-Description": "Nikon TE2000 hub", "528 AO-MinVolts": "0.0000", "COM4-Parity": "None", "Summary": { "Slices": 11, "ChContrastMin": [ 0, 0, 0, 0, 0, 0 ], "PixelAspect": 1, "SlicesFirst": true, "Interval_ms": 0, "Time": "2019-12-17 03:59:33 -0500", "Prefix": "seq_7", "InitialPositionList": [ { "Label": "Pos0", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [149.91], "Focus": [16657.7], "XYStage": [ 71629.7, 19152.9 ] }, "GridColumnIndex": 0 }, { "Label": "Pos1", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [190.82], "Focus": [16485.4], "XYStage": [ 65702.3, 19396.600000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos2", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [194.69], "Focus": [16446.8], "XYStage": [ 59880.5, 19007.8 ] }, "GridColumnIndex": 0 }, { "Label": "Pos3", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [145.04], "Focus": [16446.6], "XYStage": [ 53616.9, 18818.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos4", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [169.57500000000002], "Focus": [16446.5], "XYStage": [ 47662.8, 18817.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos5", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [131.805], "Focus": [16446.4], "XYStage": [ 41918.8, 18298.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos6", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [113.145], "Focus": [16446.5], "XYStage": [ 36129.9, 18895.8 ] }, "GridColumnIndex": 0 }, { "Label": "Pos7", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [141.005], "Focus": [16446.6], "XYStage": [ 30299.100000000002, 18895.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos8", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [140.97], "Focus": [16446.5], "XYStage": [ 23952.100000000002, 19156 ] }, "GridColumnIndex": 0 }, { "Label": "Pos9", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [184.57500000000002], "Focus": [16677.9], "XYStage": [ 72866.8, -14809.300000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos10", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [167.95000000000002], "Focus": [16557.9], "XYStage": [ 66515.6, -14371.300000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos11", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [168], "Focus": [16618.9], "XYStage": [ 59724.3, -13964.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos12", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [203.205], "Focus": [16779.6], "XYStage": [ 53862.3, -13494.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos13", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [203.20000000000002], "Focus": [16749], "XYStage": [ 48108.3, -13805.400000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos14", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [203.19], "Focus": [16675.9], "XYStage": [ 41645, -13804.900000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos15", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [203.21], "Focus": [16675.7], "XYStage": [ 35352.6, -13805.6 ] }, "GridColumnIndex": 0 }, { "Label": "Pos16", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [203.225], "Focus": [16675.8], "XYStage": [ 29298, -13447.400000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos17", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [203.215], "Focus": [16675.8], "XYStage": [ 23269.100000000002, -13012.300000000001 ] }, "GridColumnIndex": 0 } ], "z-step_um": 10, "GridRow": 0, "BitDepth": 16, "Depth": 2, "Channels": 6, "Source": "Micro-Manager", "ChColors": [ -16711681, -256, -65281, -1, -1, -16776961 ], "MetadataVersion": 10, "KeepShutterOpenChannels": true, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536, 65536 ], "CustomIntervals_ms": [], "Height": 1200, "MicroManagerVersion": "1.4.23 20180219", "GridColumn": 0, "UUID": "3ad8801b-5699-40d6-813b-4ad840863e38", "PixelType": "GRAY16", "Width": 1200, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\Yan\\20191216-YC113-S11S12-miseq", "Frames": 1, "Comment": "", "UserName": "Nikon", "PixelSize_um": 1.1, "ROI": [ 0, 0, 1200, 1200 ], "Date": "2019-12-17", "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC", "P-DAPI-high" ], "KeepShutterOpenSlices": true, "IJType": 1, "ComputerName": "ZadorConfocal2", "Positions": 18, "PositionIndex": 17, "CameraTimeout": 20000, "TimeFirst": false }, "640 AO-Name": "AnalogIO", "Vincent-D3-Command": "Close" }, "FrameKey-0-2-4": { "COM4-StopBits": "1", "528 AO-MaxVolts": "5.0000", "XLIGHT Excitation Wheel-State": "1", "470 Shutter-Name": "AnalogIO", "Core-Shutter": "640", "555 AO-Demo": "No", "XLIGHT Dichroic-State": "2", "XYStage-StepSize-Y": "0.1000", "ZPositionUm": 193.215, "XYStage-StepSize-X": "0.1000", "PRIME-FirmwareVersion": "20.28", "PRIME-ClearCycles": "2", "520-Name": "DA Shutter", "405 Shutter-Name": "AnalogIO", "PIEZO-StepSize": "0.0050", "PRIME-ClearMode": "Pre-Exposure", "470 AO-MinVolts": "0.0000", "405 AO-SupportsTriggering": "1", "CameraChannelIndex": 0, "XYStage-StartSpeed": "500.0000", "COM9-StopBits": "2", "405 AO-Description": "NI DAC", "COM4-DelayBetweenCharsMs": "0.0000", "445 AO-Block voltage": "false", "520 AO-TriggerInputLine": "/Dev1/PFI0", "555 AO-Description": "NI DAC", "520 AO-TriggerSampleRate": "100000", "COM4-Verbose": "1", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM4-Name": "COM4", "405 Shutter-Volts": "0.0000", "Vincent-D3-Port": "COM1", "PRIME-PP 4 MIN ADU AFFECTED": "200", "470 Shutter-MinVolts": "0.0000", "PRIME-PP 3 ENABLED": "Yes", "470 AO-Description": "NI DAC", "PRIME-Description": "PVCAM API device adapter", "520 Shutter-MaxVolts": "5.0000", "520 Shutter-Volts": "0.0000", "528 AO-Sequenceable": "1", "PRIME-Binning": "1x1", "555 Shutter-MaxVolts": "5.0000", "528 Shutter-TriggerSequenceLength": "1000", "405 Shutter-Block voltage": "false", "XLIGHT Spin motor-State": "1", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "Vincent-D3-Time to open (ms)": "35.0000", "Vincent-D3-Time to close (ms)": "35.0000", "445 AO-TriggerSampleRate": "100000", "640 Shutter-IOChannel": "Dev1/ao14", "555 AO-Sequenceable": "1", "520 AO-Name": "AnalogIO", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "470 AO-Block voltage": "false", "COM1-Verbose": "1", "COM9-AnswerTimeout": "10000.0000", "528 Shutter-Name": "AnalogIO", "PRIME-Trigger Timeout (secs)": "10", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "NextFrame": 0, "470 AO-TriggerSequenceLength": "1000", "PRIME-TransposeXY": "0", "445 AO-TriggerSequenceLength": "1000", "COM1-Name": "COM1", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "COM9-DataBits": "8", "COM1-AnswerTimeout": "500.0000", "Camera": "", "TE2000-Version": "V2.50-V2.50-V2.10", "528 Shutter-Volts": "0.0000", "XLIGHT Hub-Name": "XLIGHT Hub", "PIEZO-Description": "Ludl stage driver adapter", "PositionName": "Pos17", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSampleRate": "100000", "PRIME-FanSpeedSetpoint": "High", "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "405 AO-TriggerSampleRate": "100000", "470 Shutter-Block voltage": "false", "470-State": "0", "COM3-Parity": "None", "405 AO-Volts": "0.0000", "PRIME-TransposeMirrorY": "0", "PRIME-ChipName": "GS144BSI", "470 AO-Volts": "0.0000", "PRIME-TransposeMirrorX": "0", "445 Shutter-SupportsTriggering": "0", "470 Shutter-SupportsTriggering": "0", "XLIGHT Hub-Description": "XLIGHT hub", "PRIME-PreampOffLimit": "10000", "470 AO-Sequenceable": "1", "528 Shutter-MaxVolts": "5.0000", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "Core-XYStage": "XYStage", "470 AO-TriggerSampleRate": "100000", "PRIME-Timing-PreTriggerDelayNs": "24672000.0000", "528-DA Device": "528 Shutter", "Focus-Description": "Nikon TE2000 focus adapter", "FilterBlock-State": "5", "520 AO-Demo": "No", "COM3-DelayBetweenCharsMs": "0.0000", "470 Shutter-Volts": "0.0000", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "PRIME-PP 2 THRESHOLD": "125", "PRIME-PP 4 THRESHOLD": "75", "XYStage-Acceleration": "4.0000", "470 Shutter-Demo": "No", "PRIME-Name": "PMPCIECam00", "640-DA Device": "640 Shutter", "405 AO-Block voltage": "false", "ElapsedTime-ms": 730809, "PRIME-Offset": "100", "445 AO-IOChannel": "Dev1/ao1", "640 AO-MaxVolts": "5.0000", "PositionIndex": 17, "PRIME-PP 1 ENABLED": "Yes", "COM1-BaudRate": "9600", "640-State": "1", "COM3-Verbose": "1", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "LudlController-TransmissionDelay": "0", "PRIME-FullWellCapacity": "62000", "PRIME-SMARTStreamingEnabled": "No", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "640 Shutter-Description": "NI DAC", "528-Description": "DA device that is used as a shutter", "COM4-Description": "Serial port driver (boost:asio)", "405 Shutter-Description": "NI DAC", "TE2000-Name": "TE2000", "528 Shutter-Description": "NI DAC", "COM9-Description": "Serial port driver (boost:asio)", "LudlController-Name": "LudlController", "445 Shutter-TriggerSequenceLength": "1000", "PRIME-SerialNumber": "A18B203008", "445 AO-Volts": "0.0000", "PRIME-X-dimension": "1200", "PRIME-ShutterCloseDelay": "0", "XLIGHT Disk slider-State": "2", "Position": "Default", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 AO-IOChannel": "Dev1/ao5", "520 AO-IOChannel": "Dev1/ao4", "528 AO-TriggerSequenceLength": "1000", "405 Shutter-MaxVolts": "5.0000", "470-DA Device": "470 Shutter", "PRIME-FTCapable": "No", "Core-ChannelGroup": "Channels", "555 AO-SupportsTriggering": "0", "PRIME-Y-dimension": "1200", "Frame": 0, "555 Shutter-Sequenceable": "1", "PRIME-Timing-ExposureTimeNs": "499998000.0000", "Core-ImageProcessor": "", "528-State": "0", "520 AO-Block voltage": "false", "XLIGHT Excitation Wheel-Label": "QUAD", "640 Shutter-Demo": "No", "COM3-StopBits": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "520 Shutter-IOChannel": "Dev1/ao11", "Width": 1200, "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-SupportsTriggering": "0", "640 AO-TriggerSampleRate": "100000", "520-Description": "DA device that is used as a shutter", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "405 Shutter-Sequenceable": "1", "405 Shutter-SupportsTriggering": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-TriggerSampleRate": "100000", "640 Shutter-MaxVolts": "5.0000", "555 AO-MaxVolts": "5.0000", "520 AO-TriggerSequenceLength": "1000", "COM3-BaudRate": "9600", "COM9-BaudRate": "9600", "Vincent-D3-Channel #": "Ch. #1", "PRIME-PMode": "Normal", "OpticalPath-Label": "Left(100)", "BitDepth": 16, "555 AO-TriggerSequenceLength": "1000", "445 Shutter-Description": "NI DAC", "405 AO-Sequenceable": "1", "PRIME-AcquisitionMethod": "Callbacks", "555 AO-Name": "AnalogIO", "PRIME-ShutterMode": "Pre-Exposure", "COM3-Handshaking": "Off", "445 AO-SupportsTriggering": "0", "ROI": "0-0-1200-1200", "COM9-Verbose": "1", "PRIME-Color": "OFF", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "PRIME-ShutterOpenDelay": "0", "COM1-Parity": "None", "Slice": 4, "SlicePosition": -10, "COM9-DelayBetweenCharsMs": "0.0000", "555-Name": "DA Shutter", "PIEZO-LudlSingleAxisName": "B", "XLIGHT Emission Wheel-Label": "679/29 (A)", "640 Shutter-Name": "AnalogIO", "640 AO-Sequenceable": "1", "520 Shutter-Demo": "No", "528 Shutter-Demo": "No", "470-Name": "DA Shutter", "520-DA Device": "520 Shutter", "XYStage-Description": "Ludl XY stage driver adapter", "445 Shutter-MinVolts": "0.0000", "528 AO-TriggerSampleRate": "100000", "405 AO-MinVolts": "0.0000", "405 Shutter-TriggerSequenceLength": "1000", "Height": 1200, "555 AO-Block voltage": "false", "640 Shutter-Volts": "5.0000", "PixelType": "GRAY16", "445 Shutter-Block voltage": "false", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "520 Shutter-TriggerSequenceLength": "1000", "COM9-Name": "COM9", "Core-SLM": "", "555 AO-Volts": "0.0000", "555 Shutter-Block voltage": "false", "XYStage-Speed": "1800.0000", "520 AO-MaxVolts": "5.0000", "528 Shutter-SupportsTriggering": "0", "COM1-Description": "Serial port driver (boost:asio)", "PRIME-MetadataEnabled": "No", "520 Shutter-MinVolts": "0.0000", "470 Shutter-Sequenceable": "1", "PRIME-PreampDelay": "0", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-MinVolts": "0.0000", "ChannelIndex": 2, "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Core-AutoShutter": "1", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "Exposure-ms": 320, "PRIME-PP 2 ENABLED": "Yes", "640 Shutter-TriggerSampleRate": "100000", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "470 Shutter-IOChannel": "Dev1/ao10", "405 AO-Demo": "No", "PixelSizeUm": 1.1, "470 AO-Demo": "No", "470 Shutter-MaxVolts": "5.0000", "Vincent-D3-Name": "Vincent-D3", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FileName": "img_000000000_Il-A_004.tif", "LudlController-Reset": "Operate", "PRIME-Exposure": "320.00", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "445-Name": "DA Shutter", "555 Shutter-Name": "AnalogIO", "520 Shutter-TriggerSampleRate": "100000", "445 Shutter-IOChannel": "Dev1/ao9", "PRIME-TransposeCorrection": "0", "XYStage-StepSize": "0.1000", "445-Description": "DA device that is used as a shutter", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 AO-IOChannel": "Dev1/ao7", "520 Shutter-Name": "AnalogIO", "COM3-DataBits": "8", "640 AO-Volts": "4.5000", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "555 Shutter-IOChannel": "Dev1/ao13", "WaitInterval": null, "520 Shutter-Sequenceable": "1", "YPositionUm": -13012.300000000001, "445-State": "0", "445-DA Device": "445 Shutter", "PRIME-CircularBufferAutoSize": "ON", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "555 AO-TriggerSampleRate": "100000", "445 Shutter-Volts": "0.0000", "XLIGHT Hub-Port": "COM3", "Core-AutoFocus": "", "COM4-Handshaking": "Off", "528 Shutter-MinVolts": "0.0000", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "445 Shutter-Name": "AnalogIO", "528 Shutter-Sequenceable": "1", "XYStage-TransposeMirrorX": "0", "XYStage-TransposeMirrorY": "0", "PRIME-ReadoutRate": "100MHz 16bit", "XYStage-Name": "Stage", "640 AO-SupportsTriggering": "0", "640-Name": "DA Shutter", "640 Shutter-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "640 AO-Block voltage": "false", "445 Shutter-Sequenceable": "1", "470 AO-MaxVolts": "5.0000", "COM4-BaudRate": "9600", "470-Description": "DA device that is used as a shutter", "520 Shutter-Description": "NI DAC", "PRIME-CCDTemperature": "-14.9200", "Time": "2019-12-17 03:59:47 -0500", "640 AO-TriggerSequenceLength": "1000", "470 AO-Name": "AnalogIO", "XLIGHT Disk slider-Label": "Disk Pos 40um", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "Binning": "1x1", "TE2000-ModelType": "1", "COM3-AnswerTimeout": "500.0000", "555 Shutter-SupportsTriggering": "0", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "TE2000-Port": "COM4", "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Description": "NI DAC", "COM4-AnswerTimeout": "500.0000", "COM1-DelayBetweenCharsMs": "0.0000", "FilterBlock-Label": "Confocal", "445 AO-Name": "AnalogIO", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-Timing-ReadoutTimeNs": "24672000.0000", "528 AO-Demo": "No", "520 AO-Sequenceable": "1", "555-Description": "DA device that is used as a shutter", "405-DA Device": "405 Shutter", "405 AO-TriggerSequenceLength": "1000", "640 AO-MinVolts": "0.0000", "640 Shutter-Block voltage": "false", "FrameIndex": 0, "PRIME-Port": "CMOS", "555 AO-MinVolts": "0.0000", "COM3-Description": "Serial port driver (boost:asio)", "528 AO-Description": "NI DAC", "555 AO-IOChannel": "Dev1/ao6", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "555 AO-TriggerInputLine": "/Dev1/PFI0", "PRIME-ActualInterval-ms": "84.6200", "LudlController-Port": "COM9", "445 Shutter-Demo": "No", "PRIME-Trigger-Expose Out-Mux": "1", "SliceIndex": 4, "PRIME-CCDTemperatureSetPoint": "-15.0000", "405 Shutter-MinVolts": "0.0000", "PRIME-ExposeOutMode": "First Row", "AxisPositions": null, "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Volts": "0.0000", "528-Name": "DA Shutter", "555-State": "0", "XLIGHT Dichroic-Label": "Di03-R635-t1", "445 AO-Sequenceable": "1", "405-Name": "DA Shutter", "405 AO-IOChannel": "Dev1/ao0", "Core-Camera": "PRIME", "Focus-Name": "Focus", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "405 Shutter-IOChannel": "Dev1/ao8", "640 AO-Demo": "No", "640 Shutter-SupportsTriggering": "0", "445 AO-Demo": "No", "555-DA Device": "555 Shutter", "470 Shutter-Description": "NI DAC", "528 AO-Volts": "0.0000", "COM1-DataBits": "8", "Vincent-D3-Address": "x", "520 AO-SupportsTriggering": "0", "405-Description": "DA device that is used as a shutter", "640 Shutter-MinVolts": "0.0000", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "405 AO-Name": "AnalogIO", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "640 AO-TriggerInputLine": "/Dev1/PFI0", "405 AO-TriggerInputLine": "/Dev1/PFI0", "640-Description": "DA device that is used as a shutter", "445 Shutter-TriggerSampleRate": "100000", "470 Shutter-TriggerSequenceLength": "1000", "COM1-StopBits": "1", "520 Shutter-SupportsTriggering": "0", "405-State": "0", "Core-Initialize": "1", "XLIGHT Emission Wheel-State": "3", "520 AO-Description": "NI DAC", "528 AO-TriggerInputLine": "/Dev1/PFI0", "445 Shutter-MaxVolts": "5.0000", "COM9-Handshaking": "Off", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "XLIGHT Spin motor-Label": "On (spinning)", "PRIME-PP 5 ENABLED": "No", "UUID": "2060161a-8262-4b5e-b18c-d2343791e925", "PRIME-TriggerMode": "Internal Trigger", "COM1-Handshaking": "Off", "PRIME-Timing-PostTriggerDelayNs": "49344000.0000", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "Focus-Position": "16732.9000", "528 Shutter-Block voltage": "false", "Channel": "Il-A", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "555 Shutter-Demo": "No", "405 Shutter-Demo": "No", "528 AO-Name": "AnalogIO", "520-State": "0", "Core-Galvo": "", "405 Shutter-TriggerSampleRate": "100000", "Focus-FocusStepSize_(nm)": "100", "PRIME-PixelType": "16bit", "COM4-DataBits": "8", "470 AO-IOChannel": "Dev1/ao2", "520 AO-MinVolts": "0.0000", "COM9-Parity": "None", "PRIME-CircularBufferEnabled": "ON", "PRIME-PP 6 Reset": "No", "640 AO-Description": "NI DAC", "Source": "PRIME", "445 AO-MinVolts": "0.0000", "PIEZO-Name": "Stage", "445 AO-Description": "NI DAC", "PRIME-CircularBufferFrameCount": "46", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "520 AO-Volts": "0.0000", "528 Shutter-TriggerSampleRate": "100000", "XPositionUm": 23269.100000000002, "OpticalPath-State": "4", "PRIME-PP 3 THRESHOLD": "80", "PRIME-PVCAM Version": "3.7.1", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Gain": "1-HDR", "640 Shutter-TriggerSequenceLength": "1000", "528 AO-SupportsTriggering": "0", "445 AO-TriggerInputLine": "/Dev1/PFI0", "OpticalPath-Name": "OpticalPath", "PRIME-PP 4 ENABLED": "Yes", "PRIME-PVCAM Adapter Version": "1.3.29", "COM3-Name": "COM3", "405 AO-MaxVolts": "5.0000", "520 Shutter-Block voltage": "false", "PRIME-Timing-ClearingTimeNs": "49344000.0000", "Core-Focus": "PIEZO", "528 AO-Block voltage": "false", "TE2000-Description": "Nikon TE2000 hub", "528 AO-MinVolts": "0.0000", "COM4-Parity": "None", "Summary": { "Slices": 11, "ChContrastMin": [ 0, 0, 0, 0, 0, 0 ], "PixelAspect": 1, "SlicesFirst": true, "Interval_ms": 0, "Time": "2019-12-17 03:59:33 -0500", "Prefix": "seq_7", "InitialPositionList": [ { "Label": "Pos0", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [149.91], "Focus": [16657.7], "XYStage": [ 71629.7, 19152.9 ] }, "GridColumnIndex": 0 }, { "Label": "Pos1", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [190.82], "Focus": [16485.4], "XYStage": [ 65702.3, 19396.600000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos2", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [194.69], "Focus": [16446.8], "XYStage": [ 59880.5, 19007.8 ] }, "GridColumnIndex": 0 }, { "Label": "Pos3", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [145.04], "Focus": [16446.6], "XYStage": [ 53616.9, 18818.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos4", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [169.57500000000002], "Focus": [16446.5], "XYStage": [ 47662.8, 18817.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos5", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [131.805], "Focus": [16446.4], "XYStage": [ 41918.8, 18298.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos6", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [113.145], "Focus": [16446.5], "XYStage": [ 36129.9, 18895.8 ] }, "GridColumnIndex": 0 }, { "Label": "Pos7", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [141.005], "Focus": [16446.6], "XYStage": [ 30299.100000000002, 18895.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos8", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [140.97], "Focus": [16446.5], "XYStage": [ 23952.100000000002, 19156 ] }, "GridColumnIndex": 0 }, { "Label": "Pos9", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [184.57500000000002], "Focus": [16677.9], "XYStage": [ 72866.8, -14809.300000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos10", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [167.95000000000002], "Focus": [16557.9], "XYStage": [ 66515.6, -14371.300000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos11", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [168], "Focus": [16618.9], "XYStage": [ 59724.3, -13964.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos12", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [203.205], "Focus": [16779.6], "XYStage": [ 53862.3, -13494.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos13", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [203.20000000000002], "Focus": [16749], "XYStage": [ 48108.3, -13805.400000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos14", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [203.19], "Focus": [16675.9], "XYStage": [ 41645, -13804.900000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos15", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [203.21], "Focus": [16675.7], "XYStage": [ 35352.6, -13805.6 ] }, "GridColumnIndex": 0 }, { "Label": "Pos16", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [203.225], "Focus": [16675.8], "XYStage": [ 29298, -13447.400000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos17", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [203.215], "Focus": [16675.8], "XYStage": [ 23269.100000000002, -13012.300000000001 ] }, "GridColumnIndex": 0 } ], "z-step_um": 10, "GridRow": 0, "BitDepth": 16, "Depth": 2, "Channels": 6, "Source": "Micro-Manager", "ChColors": [ -16711681, -256, -65281, -1, -1, -16776961 ], "MetadataVersion": 10, "KeepShutterOpenChannels": true, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536, 65536 ], "CustomIntervals_ms": [], "Height": 1200, "MicroManagerVersion": "1.4.23 20180219", "GridColumn": 0, "UUID": "3ad8801b-5699-40d6-813b-4ad840863e38", "PixelType": "GRAY16", "Width": 1200, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\Yan\\20191216-YC113-S11S12-miseq", "Frames": 1, "Comment": "", "UserName": "Nikon", "PixelSize_um": 1.1, "ROI": [ 0, 0, 1200, 1200 ], "Date": "2019-12-17", "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC", "P-DAPI-high" ], "KeepShutterOpenSlices": true, "IJType": 1, "ComputerName": "ZadorConfocal2", "Positions": 18, "PositionIndex": 17, "CameraTimeout": 20000, "TimeFirst": false }, "640 AO-Name": "AnalogIO", "Vincent-D3-Command": "Close" }, "FrameKey-0-2-5": { "COM4-StopBits": "1", "528 AO-MaxVolts": "5.0000", "XLIGHT Excitation Wheel-State": "1", "470 Shutter-Name": "AnalogIO", "Core-Shutter": "640", "555 AO-Demo": "No", "XLIGHT Dichroic-State": "2", "XYStage-StepSize-Y": "0.1000", "ZPositionUm": 203.215, "XYStage-StepSize-X": "0.1000", "PRIME-FirmwareVersion": "20.28", "PRIME-ClearCycles": "2", "520-Name": "DA Shutter", "405 Shutter-Name": "AnalogIO", "PIEZO-StepSize": "0.0050", "PRIME-ClearMode": "Pre-Exposure", "470 AO-MinVolts": "0.0000", "405 AO-SupportsTriggering": "1", "CameraChannelIndex": 0, "XYStage-StartSpeed": "500.0000", "COM9-StopBits": "2", "405 AO-Description": "NI DAC", "COM4-DelayBetweenCharsMs": "0.0000", "445 AO-Block voltage": "false", "520 AO-TriggerInputLine": "/Dev1/PFI0", "555 AO-Description": "NI DAC", "520 AO-TriggerSampleRate": "100000", "COM4-Verbose": "1", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM4-Name": "COM4", "405 Shutter-Volts": "0.0000", "Vincent-D3-Port": "COM1", "PRIME-PP 4 MIN ADU AFFECTED": "200", "470 Shutter-MinVolts": "0.0000", "PRIME-PP 3 ENABLED": "Yes", "470 AO-Description": "NI DAC", "PRIME-Description": "PVCAM API device adapter", "520 Shutter-MaxVolts": "5.0000", "520 Shutter-Volts": "0.0000", "528 AO-Sequenceable": "1", "PRIME-Binning": "1x1", "555 Shutter-MaxVolts": "5.0000", "528 Shutter-TriggerSequenceLength": "1000", "405 Shutter-Block voltage": "false", "XLIGHT Spin motor-State": "1", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "Vincent-D3-Time to open (ms)": "35.0000", "Vincent-D3-Time to close (ms)": "35.0000", "445 AO-TriggerSampleRate": "100000", "640 Shutter-IOChannel": "Dev1/ao14", "555 AO-Sequenceable": "1", "520 AO-Name": "AnalogIO", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "470 AO-Block voltage": "false", "COM1-Verbose": "1", "COM9-AnswerTimeout": "10000.0000", "528 Shutter-Name": "AnalogIO", "PRIME-Trigger Timeout (secs)": "10", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "NextFrame": 0, "470 AO-TriggerSequenceLength": "1000", "PRIME-TransposeXY": "0", "445 AO-TriggerSequenceLength": "1000", "COM1-Name": "COM1", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "COM9-DataBits": "8", "COM1-AnswerTimeout": "500.0000", "Camera": "", "TE2000-Version": "V2.50-V2.50-V2.10", "528 Shutter-Volts": "0.0000", "XLIGHT Hub-Name": "XLIGHT Hub", "PIEZO-Description": "Ludl stage driver adapter", "PositionName": "Pos17", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSampleRate": "100000", "PRIME-FanSpeedSetpoint": "High", "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "405 AO-TriggerSampleRate": "100000", "470 Shutter-Block voltage": "false", "470-State": "0", "COM3-Parity": "None", "405 AO-Volts": "0.0000", "PRIME-TransposeMirrorY": "0", "PRIME-ChipName": "GS144BSI", "470 AO-Volts": "0.0000", "PRIME-TransposeMirrorX": "0", "445 Shutter-SupportsTriggering": "0", "470 Shutter-SupportsTriggering": "0", "XLIGHT Hub-Description": "XLIGHT hub", "PRIME-PreampOffLimit": "10000", "470 AO-Sequenceable": "1", "528 Shutter-MaxVolts": "5.0000", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "Core-XYStage": "XYStage", "470 AO-TriggerSampleRate": "100000", "PRIME-Timing-PreTriggerDelayNs": "24672000.0000", "528-DA Device": "528 Shutter", "Focus-Description": "Nikon TE2000 focus adapter", "FilterBlock-State": "5", "520 AO-Demo": "No", "COM3-DelayBetweenCharsMs": "0.0000", "470 Shutter-Volts": "0.0000", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "PRIME-PP 2 THRESHOLD": "125", "PRIME-PP 4 THRESHOLD": "75", "XYStage-Acceleration": "4.0000", "470 Shutter-Demo": "No", "PRIME-Name": "PMPCIECam00", "640-DA Device": "640 Shutter", "405 AO-Block voltage": "false", "ElapsedTime-ms": 731368, "PRIME-Offset": "100", "445 AO-IOChannel": "Dev1/ao1", "640 AO-MaxVolts": "5.0000", "PositionIndex": 17, "PRIME-PP 1 ENABLED": "Yes", "COM1-BaudRate": "9600", "640-State": "1", "COM3-Verbose": "1", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "LudlController-TransmissionDelay": "0", "PRIME-FullWellCapacity": "62000", "PRIME-SMARTStreamingEnabled": "No", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "640 Shutter-Description": "NI DAC", "528-Description": "DA device that is used as a shutter", "COM4-Description": "Serial port driver (boost:asio)", "405 Shutter-Description": "NI DAC", "TE2000-Name": "TE2000", "528 Shutter-Description": "NI DAC", "COM9-Description": "Serial port driver (boost:asio)", "LudlController-Name": "LudlController", "445 Shutter-TriggerSequenceLength": "1000", "PRIME-SerialNumber": "A18B203008", "445 AO-Volts": "0.0000", "PRIME-X-dimension": "1200", "PRIME-ShutterCloseDelay": "0", "XLIGHT Disk slider-State": "2", "Position": "Default", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 AO-IOChannel": "Dev1/ao5", "520 AO-IOChannel": "Dev1/ao4", "528 AO-TriggerSequenceLength": "1000", "405 Shutter-MaxVolts": "5.0000", "470-DA Device": "470 Shutter", "PRIME-FTCapable": "No", "Core-ChannelGroup": "Channels", "555 AO-SupportsTriggering": "0", "PRIME-Y-dimension": "1200", "Frame": 0, "555 Shutter-Sequenceable": "1", "PRIME-Timing-ExposureTimeNs": "499998000.0000", "Core-ImageProcessor": "", "528-State": "0", "520 AO-Block voltage": "false", "XLIGHT Excitation Wheel-Label": "QUAD", "640 Shutter-Demo": "No", "COM3-StopBits": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "520 Shutter-IOChannel": "Dev1/ao11", "Width": 1200, "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-SupportsTriggering": "0", "640 AO-TriggerSampleRate": "100000", "520-Description": "DA device that is used as a shutter", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "405 Shutter-Sequenceable": "1", "405 Shutter-SupportsTriggering": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-TriggerSampleRate": "100000", "640 Shutter-MaxVolts": "5.0000", "555 AO-MaxVolts": "5.0000", "520 AO-TriggerSequenceLength": "1000", "COM3-BaudRate": "9600", "COM9-BaudRate": "9600", "Vincent-D3-Channel #": "Ch. #1", "PRIME-PMode": "Normal", "OpticalPath-Label": "Left(100)", "BitDepth": 16, "555 AO-TriggerSequenceLength": "1000", "445 Shutter-Description": "NI DAC", "405 AO-Sequenceable": "1", "PRIME-AcquisitionMethod": "Callbacks", "555 AO-Name": "AnalogIO", "PRIME-ShutterMode": "Pre-Exposure", "COM3-Handshaking": "Off", "445 AO-SupportsTriggering": "0", "ROI": "0-0-1200-1200", "COM9-Verbose": "1", "PRIME-Color": "OFF", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "PRIME-ShutterOpenDelay": "0", "COM1-Parity": "None", "Slice": 5, "SlicePosition": 0, "COM9-DelayBetweenCharsMs": "0.0000", "555-Name": "DA Shutter", "PIEZO-LudlSingleAxisName": "B", "XLIGHT Emission Wheel-Label": "679/29 (A)", "640 Shutter-Name": "AnalogIO", "640 AO-Sequenceable": "1", "520 Shutter-Demo": "No", "528 Shutter-Demo": "No", "470-Name": "DA Shutter", "520-DA Device": "520 Shutter", "XYStage-Description": "Ludl XY stage driver adapter", "445 Shutter-MinVolts": "0.0000", "528 AO-TriggerSampleRate": "100000", "405 AO-MinVolts": "0.0000", "405 Shutter-TriggerSequenceLength": "1000", "Height": 1200, "555 AO-Block voltage": "false", "640 Shutter-Volts": "5.0000", "PixelType": "GRAY16", "445 Shutter-Block voltage": "false", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "520 Shutter-TriggerSequenceLength": "1000", "COM9-Name": "COM9", "Core-SLM": "", "555 AO-Volts": "0.0000", "555 Shutter-Block voltage": "false", "XYStage-Speed": "1800.0000", "520 AO-MaxVolts": "5.0000", "528 Shutter-SupportsTriggering": "0", "COM1-Description": "Serial port driver (boost:asio)", "PRIME-MetadataEnabled": "No", "520 Shutter-MinVolts": "0.0000", "470 Shutter-Sequenceable": "1", "PRIME-PreampDelay": "0", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-MinVolts": "0.0000", "ChannelIndex": 2, "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Core-AutoShutter": "1", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "Exposure-ms": 320, "PRIME-PP 2 ENABLED": "Yes", "640 Shutter-TriggerSampleRate": "100000", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "470 Shutter-IOChannel": "Dev1/ao10", "405 AO-Demo": "No", "PixelSizeUm": 1.1, "470 AO-Demo": "No", "470 Shutter-MaxVolts": "5.0000", "Vincent-D3-Name": "Vincent-D3", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FileName": "img_000000000_Il-A_005.tif", "LudlController-Reset": "Operate", "PRIME-Exposure": "320.00", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "445-Name": "DA Shutter", "555 Shutter-Name": "AnalogIO", "520 Shutter-TriggerSampleRate": "100000", "445 Shutter-IOChannel": "Dev1/ao9", "PRIME-TransposeCorrection": "0", "XYStage-StepSize": "0.1000", "445-Description": "DA device that is used as a shutter", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 AO-IOChannel": "Dev1/ao7", "520 Shutter-Name": "AnalogIO", "COM3-DataBits": "8", "640 AO-Volts": "4.5000", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "555 Shutter-IOChannel": "Dev1/ao13", "WaitInterval": null, "520 Shutter-Sequenceable": "1", "YPositionUm": -13012.300000000001, "445-State": "0", "445-DA Device": "445 Shutter", "PRIME-CircularBufferAutoSize": "ON", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "555 AO-TriggerSampleRate": "100000", "445 Shutter-Volts": "0.0000", "XLIGHT Hub-Port": "COM3", "Core-AutoFocus": "", "COM4-Handshaking": "Off", "528 Shutter-MinVolts": "0.0000", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "445 Shutter-Name": "AnalogIO", "528 Shutter-Sequenceable": "1", "XYStage-TransposeMirrorX": "0", "XYStage-TransposeMirrorY": "0", "PRIME-ReadoutRate": "100MHz 16bit", "XYStage-Name": "Stage", "640 AO-SupportsTriggering": "0", "640-Name": "DA Shutter", "640 Shutter-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "640 AO-Block voltage": "false", "445 Shutter-Sequenceable": "1", "470 AO-MaxVolts": "5.0000", "COM4-BaudRate": "9600", "470-Description": "DA device that is used as a shutter", "520 Shutter-Description": "NI DAC", "PRIME-CCDTemperature": "-14.9200", "Time": "2019-12-17 03:59:48 -0500", "640 AO-TriggerSequenceLength": "1000", "470 AO-Name": "AnalogIO", "XLIGHT Disk slider-Label": "Disk Pos 40um", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "Binning": "1x1", "TE2000-ModelType": "1", "COM3-AnswerTimeout": "500.0000", "555 Shutter-SupportsTriggering": "0", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "TE2000-Port": "COM4", "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Description": "NI DAC", "COM4-AnswerTimeout": "500.0000", "COM1-DelayBetweenCharsMs": "0.0000", "FilterBlock-Label": "Confocal", "445 AO-Name": "AnalogIO", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-Timing-ReadoutTimeNs": "24672000.0000", "528 AO-Demo": "No", "520 AO-Sequenceable": "1", "555-Description": "DA device that is used as a shutter", "405-DA Device": "405 Shutter", "405 AO-TriggerSequenceLength": "1000", "640 AO-MinVolts": "0.0000", "640 Shutter-Block voltage": "false", "FrameIndex": 0, "PRIME-Port": "CMOS", "555 AO-MinVolts": "0.0000", "COM3-Description": "Serial port driver (boost:asio)", "528 AO-Description": "NI DAC", "555 AO-IOChannel": "Dev1/ao6", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "555 AO-TriggerInputLine": "/Dev1/PFI0", "PRIME-ActualInterval-ms": "84.6200", "LudlController-Port": "COM9", "445 Shutter-Demo": "No", "PRIME-Trigger-Expose Out-Mux": "1", "SliceIndex": 5, "PRIME-CCDTemperatureSetPoint": "-15.0000", "405 Shutter-MinVolts": "0.0000", "PRIME-ExposeOutMode": "First Row", "AxisPositions": null, "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Volts": "0.0000", "528-Name": "DA Shutter", "555-State": "0", "XLIGHT Dichroic-Label": "Di03-R635-t1", "445 AO-Sequenceable": "1", "405-Name": "DA Shutter", "405 AO-IOChannel": "Dev1/ao0", "Core-Camera": "PRIME", "Focus-Name": "Focus", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "405 Shutter-IOChannel": "Dev1/ao8", "640 AO-Demo": "No", "640 Shutter-SupportsTriggering": "0", "445 AO-Demo": "No", "555-DA Device": "555 Shutter", "470 Shutter-Description": "NI DAC", "528 AO-Volts": "0.0000", "COM1-DataBits": "8", "Vincent-D3-Address": "x", "520 AO-SupportsTriggering": "0", "405-Description": "DA device that is used as a shutter", "640 Shutter-MinVolts": "0.0000", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "405 AO-Name": "AnalogIO", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "640 AO-TriggerInputLine": "/Dev1/PFI0", "405 AO-TriggerInputLine": "/Dev1/PFI0", "640-Description": "DA device that is used as a shutter", "445 Shutter-TriggerSampleRate": "100000", "470 Shutter-TriggerSequenceLength": "1000", "COM1-StopBits": "1", "520 Shutter-SupportsTriggering": "0", "405-State": "0", "Core-Initialize": "1", "XLIGHT Emission Wheel-State": "3", "520 AO-Description": "NI DAC", "528 AO-TriggerInputLine": "/Dev1/PFI0", "445 Shutter-MaxVolts": "5.0000", "COM9-Handshaking": "Off", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "XLIGHT Spin motor-Label": "On (spinning)", "PRIME-PP 5 ENABLED": "No", "UUID": "6c7e887e-6fea-4c4e-a609-73a8687a7efa", "PRIME-TriggerMode": "Internal Trigger", "COM1-Handshaking": "Off", "PRIME-Timing-PostTriggerDelayNs": "49344000.0000", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "Focus-Position": "16732.9000", "528 Shutter-Block voltage": "false", "Channel": "Il-A", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "555 Shutter-Demo": "No", "405 Shutter-Demo": "No", "528 AO-Name": "AnalogIO", "520-State": "0", "Core-Galvo": "", "405 Shutter-TriggerSampleRate": "100000", "Focus-FocusStepSize_(nm)": "100", "PRIME-PixelType": "16bit", "COM4-DataBits": "8", "470 AO-IOChannel": "Dev1/ao2", "520 AO-MinVolts": "0.0000", "COM9-Parity": "None", "PRIME-CircularBufferEnabled": "ON", "PRIME-PP 6 Reset": "No", "640 AO-Description": "NI DAC", "Source": "PRIME", "445 AO-MinVolts": "0.0000", "PIEZO-Name": "Stage", "445 AO-Description": "NI DAC", "PRIME-CircularBufferFrameCount": "46", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "520 AO-Volts": "0.0000", "528 Shutter-TriggerSampleRate": "100000", "XPositionUm": 23269.100000000002, "OpticalPath-State": "4", "PRIME-PP 3 THRESHOLD": "80", "PRIME-PVCAM Version": "3.7.1", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Gain": "1-HDR", "640 Shutter-TriggerSequenceLength": "1000", "528 AO-SupportsTriggering": "0", "445 AO-TriggerInputLine": "/Dev1/PFI0", "OpticalPath-Name": "OpticalPath", "PRIME-PP 4 ENABLED": "Yes", "PRIME-PVCAM Adapter Version": "1.3.29", "COM3-Name": "COM3", "405 AO-MaxVolts": "5.0000", "520 Shutter-Block voltage": "false", "PRIME-Timing-ClearingTimeNs": "49344000.0000", "Core-Focus": "PIEZO", "528 AO-Block voltage": "false", "TE2000-Description": "Nikon TE2000 hub", "528 AO-MinVolts": "0.0000", "COM4-Parity": "None", "Summary": { "Slices": 11, "ChContrastMin": [ 0, 0, 0, 0, 0, 0 ], "PixelAspect": 1, "SlicesFirst": true, "Interval_ms": 0, "Time": "2019-12-17 03:59:33 -0500", "Prefix": "seq_7", "InitialPositionList": [ { "Label": "Pos0", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [149.91], "Focus": [16657.7], "XYStage": [ 71629.7, 19152.9 ] }, "GridColumnIndex": 0 }, { "Label": "Pos1", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [190.82], "Focus": [16485.4], "XYStage": [ 65702.3, 19396.600000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos2", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [194.69], "Focus": [16446.8], "XYStage": [ 59880.5, 19007.8 ] }, "GridColumnIndex": 0 }, { "Label": "Pos3", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [145.04], "Focus": [16446.6], "XYStage": [ 53616.9, 18818.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos4", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [169.57500000000002], "Focus": [16446.5], "XYStage": [ 47662.8, 18817.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos5", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [131.805], "Focus": [16446.4], "XYStage": [ 41918.8, 18298.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos6", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [113.145], "Focus": [16446.5], "XYStage": [ 36129.9, 18895.8 ] }, "GridColumnIndex": 0 }, { "Label": "Pos7", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [141.005], "Focus": [16446.6], "XYStage": [ 30299.100000000002, 18895.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos8", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [140.97], "Focus": [16446.5], "XYStage": [ 23952.100000000002, 19156 ] }, "GridColumnIndex": 0 }, { "Label": "Pos9", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [184.57500000000002], "Focus": [16677.9], "XYStage": [ 72866.8, -14809.300000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos10", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [167.95000000000002], "Focus": [16557.9], "XYStage": [ 66515.6, -14371.300000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos11", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [168], "Focus": [16618.9], "XYStage": [ 59724.3, -13964.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos12", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [203.205], "Focus": [16779.6], "XYStage": [ 53862.3, -13494.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos13", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [203.20000000000002], "Focus": [16749], "XYStage": [ 48108.3, -13805.400000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos14", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [203.19], "Focus": [16675.9], "XYStage": [ 41645, -13804.900000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos15", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [203.21], "Focus": [16675.7], "XYStage": [ 35352.6, -13805.6 ] }, "GridColumnIndex": 0 }, { "Label": "Pos16", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [203.225], "Focus": [16675.8], "XYStage": [ 29298, -13447.400000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos17", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [203.215], "Focus": [16675.8], "XYStage": [ 23269.100000000002, -13012.300000000001 ] }, "GridColumnIndex": 0 } ], "z-step_um": 10, "GridRow": 0, "BitDepth": 16, "Depth": 2, "Channels": 6, "Source": "Micro-Manager", "ChColors": [ -16711681, -256, -65281, -1, -1, -16776961 ], "MetadataVersion": 10, "KeepShutterOpenChannels": true, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536, 65536 ], "CustomIntervals_ms": [], "Height": 1200, "MicroManagerVersion": "1.4.23 20180219", "GridColumn": 0, "UUID": "3ad8801b-5699-40d6-813b-4ad840863e38", "PixelType": "GRAY16", "Width": 1200, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\Yan\\20191216-YC113-S11S12-miseq", "Frames": 1, "Comment": "", "UserName": "Nikon", "PixelSize_um": 1.1, "ROI": [ 0, 0, 1200, 1200 ], "Date": "2019-12-17", "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC", "P-DAPI-high" ], "KeepShutterOpenSlices": true, "IJType": 1, "ComputerName": "ZadorConfocal2", "Positions": 18, "PositionIndex": 17, "CameraTimeout": 20000, "TimeFirst": false }, "640 AO-Name": "AnalogIO", "Vincent-D3-Command": "Close" }, "FrameKey-0-2-6": { "COM4-StopBits": "1", "528 AO-MaxVolts": "5.0000", "XLIGHT Excitation Wheel-State": "1", "470 Shutter-Name": "AnalogIO", "Core-Shutter": "640", "555 AO-Demo": "No", "XLIGHT Dichroic-State": "2", "XYStage-StepSize-Y": "0.1000", "ZPositionUm": 213.215, "XYStage-StepSize-X": "0.1000", "PRIME-FirmwareVersion": "20.28", "PRIME-ClearCycles": "2", "520-Name": "DA Shutter", "405 Shutter-Name": "AnalogIO", "PIEZO-StepSize": "0.0050", "PRIME-ClearMode": "Pre-Exposure", "470 AO-MinVolts": "0.0000", "405 AO-SupportsTriggering": "1", "CameraChannelIndex": 0, "XYStage-StartSpeed": "500.0000", "COM9-StopBits": "2", "405 AO-Description": "NI DAC", "COM4-DelayBetweenCharsMs": "0.0000", "445 AO-Block voltage": "false", "520 AO-TriggerInputLine": "/Dev1/PFI0", "555 AO-Description": "NI DAC", "520 AO-TriggerSampleRate": "100000", "COM4-Verbose": "1", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM4-Name": "COM4", "405 Shutter-Volts": "0.0000", "Vincent-D3-Port": "COM1", "PRIME-PP 4 MIN ADU AFFECTED": "200", "470 Shutter-MinVolts": "0.0000", "PRIME-PP 3 ENABLED": "Yes", "470 AO-Description": "NI DAC", "PRIME-Description": "PVCAM API device adapter", "520 Shutter-MaxVolts": "5.0000", "520 Shutter-Volts": "0.0000", "528 AO-Sequenceable": "1", "PRIME-Binning": "1x1", "555 Shutter-MaxVolts": "5.0000", "528 Shutter-TriggerSequenceLength": "1000", "405 Shutter-Block voltage": "false", "XLIGHT Spin motor-State": "1", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "Vincent-D3-Time to open (ms)": "35.0000", "Vincent-D3-Time to close (ms)": "35.0000", "445 AO-TriggerSampleRate": "100000", "640 Shutter-IOChannel": "Dev1/ao14", "555 AO-Sequenceable": "1", "520 AO-Name": "AnalogIO", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "470 AO-Block voltage": "false", "COM1-Verbose": "1", "COM9-AnswerTimeout": "10000.0000", "528 Shutter-Name": "AnalogIO", "PRIME-Trigger Timeout (secs)": "10", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "NextFrame": 0, "470 AO-TriggerSequenceLength": "1000", "PRIME-TransposeXY": "0", "445 AO-TriggerSequenceLength": "1000", "COM1-Name": "COM1", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "COM9-DataBits": "8", "COM1-AnswerTimeout": "500.0000", "Camera": "", "TE2000-Version": "V2.50-V2.50-V2.10", "528 Shutter-Volts": "0.0000", "XLIGHT Hub-Name": "XLIGHT Hub", "PIEZO-Description": "Ludl stage driver adapter", "PositionName": "Pos17", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSampleRate": "100000", "PRIME-FanSpeedSetpoint": "High", "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "405 AO-TriggerSampleRate": "100000", "470 Shutter-Block voltage": "false", "470-State": "0", "COM3-Parity": "None", "405 AO-Volts": "0.0000", "PRIME-TransposeMirrorY": "0", "PRIME-ChipName": "GS144BSI", "470 AO-Volts": "0.0000", "PRIME-TransposeMirrorX": "0", "445 Shutter-SupportsTriggering": "0", "470 Shutter-SupportsTriggering": "0", "XLIGHT Hub-Description": "XLIGHT hub", "PRIME-PreampOffLimit": "10000", "470 AO-Sequenceable": "1", "528 Shutter-MaxVolts": "5.0000", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "Core-XYStage": "XYStage", "470 AO-TriggerSampleRate": "100000", "PRIME-Timing-PreTriggerDelayNs": "24672000.0000", "528-DA Device": "528 Shutter", "Focus-Description": "Nikon TE2000 focus adapter", "FilterBlock-State": "5", "520 AO-Demo": "No", "COM3-DelayBetweenCharsMs": "0.0000", "470 Shutter-Volts": "0.0000", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "PRIME-PP 2 THRESHOLD": "125", "PRIME-PP 4 THRESHOLD": "75", "XYStage-Acceleration": "4.0000", "470 Shutter-Demo": "No", "PRIME-Name": "PMPCIECam00", "640-DA Device": "640 Shutter", "405 AO-Block voltage": "false", "ElapsedTime-ms": 731923, "PRIME-Offset": "100", "445 AO-IOChannel": "Dev1/ao1", "640 AO-MaxVolts": "5.0000", "PositionIndex": 17, "PRIME-PP 1 ENABLED": "Yes", "COM1-BaudRate": "9600", "640-State": "1", "COM3-Verbose": "1", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "LudlController-TransmissionDelay": "0", "PRIME-FullWellCapacity": "62000", "PRIME-SMARTStreamingEnabled": "No", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "640 Shutter-Description": "NI DAC", "528-Description": "DA device that is used as a shutter", "COM4-Description": "Serial port driver (boost:asio)", "405 Shutter-Description": "NI DAC", "TE2000-Name": "TE2000", "528 Shutter-Description": "NI DAC", "COM9-Description": "Serial port driver (boost:asio)", "LudlController-Name": "LudlController", "445 Shutter-TriggerSequenceLength": "1000", "PRIME-SerialNumber": "A18B203008", "445 AO-Volts": "0.0000", "PRIME-X-dimension": "1200", "PRIME-ShutterCloseDelay": "0", "XLIGHT Disk slider-State": "2", "Position": "Default", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 AO-IOChannel": "Dev1/ao5", "520 AO-IOChannel": "Dev1/ao4", "528 AO-TriggerSequenceLength": "1000", "405 Shutter-MaxVolts": "5.0000", "470-DA Device": "470 Shutter", "PRIME-FTCapable": "No", "Core-ChannelGroup": "Channels", "555 AO-SupportsTriggering": "0", "PRIME-Y-dimension": "1200", "Frame": 0, "555 Shutter-Sequenceable": "1", "PRIME-Timing-ExposureTimeNs": "499998000.0000", "Core-ImageProcessor": "", "528-State": "0", "520 AO-Block voltage": "false", "XLIGHT Excitation Wheel-Label": "QUAD", "640 Shutter-Demo": "No", "COM3-StopBits": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "520 Shutter-IOChannel": "Dev1/ao11", "Width": 1200, "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-SupportsTriggering": "0", "640 AO-TriggerSampleRate": "100000", "520-Description": "DA device that is used as a shutter", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "405 Shutter-Sequenceable": "1", "405 Shutter-SupportsTriggering": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-TriggerSampleRate": "100000", "640 Shutter-MaxVolts": "5.0000", "555 AO-MaxVolts": "5.0000", "520 AO-TriggerSequenceLength": "1000", "COM3-BaudRate": "9600", "COM9-BaudRate": "9600", "Vincent-D3-Channel #": "Ch. #1", "PRIME-PMode": "Normal", "OpticalPath-Label": "Left(100)", "BitDepth": 16, "555 AO-TriggerSequenceLength": "1000", "445 Shutter-Description": "NI DAC", "405 AO-Sequenceable": "1", "PRIME-AcquisitionMethod": "Callbacks", "555 AO-Name": "AnalogIO", "PRIME-ShutterMode": "Pre-Exposure", "COM3-Handshaking": "Off", "445 AO-SupportsTriggering": "0", "ROI": "0-0-1200-1200", "COM9-Verbose": "1", "PRIME-Color": "OFF", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "PRIME-ShutterOpenDelay": "0", "COM1-Parity": "None", "Slice": 6, "SlicePosition": 10, "COM9-DelayBetweenCharsMs": "0.0000", "555-Name": "DA Shutter", "PIEZO-LudlSingleAxisName": "B", "XLIGHT Emission Wheel-Label": "679/29 (A)", "640 Shutter-Name": "AnalogIO", "640 AO-Sequenceable": "1", "520 Shutter-Demo": "No", "528 Shutter-Demo": "No", "470-Name": "DA Shutter", "520-DA Device": "520 Shutter", "XYStage-Description": "Ludl XY stage driver adapter", "445 Shutter-MinVolts": "0.0000", "528 AO-TriggerSampleRate": "100000", "405 AO-MinVolts": "0.0000", "405 Shutter-TriggerSequenceLength": "1000", "Height": 1200, "555 AO-Block voltage": "false", "640 Shutter-Volts": "5.0000", "PixelType": "GRAY16", "445 Shutter-Block voltage": "false", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "520 Shutter-TriggerSequenceLength": "1000", "COM9-Name": "COM9", "Core-SLM": "", "555 AO-Volts": "0.0000", "555 Shutter-Block voltage": "false", "XYStage-Speed": "1800.0000", "520 AO-MaxVolts": "5.0000", "528 Shutter-SupportsTriggering": "0", "COM1-Description": "Serial port driver (boost:asio)", "PRIME-MetadataEnabled": "No", "520 Shutter-MinVolts": "0.0000", "470 Shutter-Sequenceable": "1", "PRIME-PreampDelay": "0", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-MinVolts": "0.0000", "ChannelIndex": 2, "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Core-AutoShutter": "1", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "Exposure-ms": 320, "PRIME-PP 2 ENABLED": "Yes", "640 Shutter-TriggerSampleRate": "100000", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "470 Shutter-IOChannel": "Dev1/ao10", "405 AO-Demo": "No", "PixelSizeUm": 1.1, "470 AO-Demo": "No", "470 Shutter-MaxVolts": "5.0000", "Vincent-D3-Name": "Vincent-D3", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FileName": "img_000000000_Il-A_006.tif", "LudlController-Reset": "Operate", "PRIME-Exposure": "320.00", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "445-Name": "DA Shutter", "555 Shutter-Name": "AnalogIO", "520 Shutter-TriggerSampleRate": "100000", "445 Shutter-IOChannel": "Dev1/ao9", "PRIME-TransposeCorrection": "0", "XYStage-StepSize": "0.1000", "445-Description": "DA device that is used as a shutter", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 AO-IOChannel": "Dev1/ao7", "520 Shutter-Name": "AnalogIO", "COM3-DataBits": "8", "640 AO-Volts": "4.5000", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "555 Shutter-IOChannel": "Dev1/ao13", "WaitInterval": null, "520 Shutter-Sequenceable": "1", "YPositionUm": -13012.300000000001, "445-State": "0", "445-DA Device": "445 Shutter", "PRIME-CircularBufferAutoSize": "ON", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "555 AO-TriggerSampleRate": "100000", "445 Shutter-Volts": "0.0000", "XLIGHT Hub-Port": "COM3", "Core-AutoFocus": "", "COM4-Handshaking": "Off", "528 Shutter-MinVolts": "0.0000", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "445 Shutter-Name": "AnalogIO", "528 Shutter-Sequenceable": "1", "XYStage-TransposeMirrorX": "0", "XYStage-TransposeMirrorY": "0", "PRIME-ReadoutRate": "100MHz 16bit", "XYStage-Name": "Stage", "640 AO-SupportsTriggering": "0", "640-Name": "DA Shutter", "640 Shutter-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "640 AO-Block voltage": "false", "445 Shutter-Sequenceable": "1", "470 AO-MaxVolts": "5.0000", "COM4-BaudRate": "9600", "470-Description": "DA device that is used as a shutter", "520 Shutter-Description": "NI DAC", "PRIME-CCDTemperature": "-14.9200", "Time": "2019-12-17 03:59:48 -0500", "640 AO-TriggerSequenceLength": "1000", "470 AO-Name": "AnalogIO", "XLIGHT Disk slider-Label": "Disk Pos 40um", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "Binning": "1x1", "TE2000-ModelType": "1", "COM3-AnswerTimeout": "500.0000", "555 Shutter-SupportsTriggering": "0", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "TE2000-Port": "COM4", "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Description": "NI DAC", "COM4-AnswerTimeout": "500.0000", "COM1-DelayBetweenCharsMs": "0.0000", "FilterBlock-Label": "Confocal", "445 AO-Name": "AnalogIO", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-Timing-ReadoutTimeNs": "24672000.0000", "528 AO-Demo": "No", "520 AO-Sequenceable": "1", "555-Description": "DA device that is used as a shutter", "405-DA Device": "405 Shutter", "405 AO-TriggerSequenceLength": "1000", "640 AO-MinVolts": "0.0000", "640 Shutter-Block voltage": "false", "FrameIndex": 0, "PRIME-Port": "CMOS", "555 AO-MinVolts": "0.0000", "COM3-Description": "Serial port driver (boost:asio)", "528 AO-Description": "NI DAC", "555 AO-IOChannel": "Dev1/ao6", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "555 AO-TriggerInputLine": "/Dev1/PFI0", "PRIME-ActualInterval-ms": "84.6200", "LudlController-Port": "COM9", "445 Shutter-Demo": "No", "PRIME-Trigger-Expose Out-Mux": "1", "SliceIndex": 6, "PRIME-CCDTemperatureSetPoint": "-15.0000", "405 Shutter-MinVolts": "0.0000", "PRIME-ExposeOutMode": "First Row", "AxisPositions": null, "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Volts": "0.0000", "528-Name": "DA Shutter", "555-State": "0", "XLIGHT Dichroic-Label": "Di03-R635-t1", "445 AO-Sequenceable": "1", "405-Name": "DA Shutter", "405 AO-IOChannel": "Dev1/ao0", "Core-Camera": "PRIME", "Focus-Name": "Focus", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "405 Shutter-IOChannel": "Dev1/ao8", "640 AO-Demo": "No", "640 Shutter-SupportsTriggering": "0", "445 AO-Demo": "No", "555-DA Device": "555 Shutter", "470 Shutter-Description": "NI DAC", "528 AO-Volts": "0.0000", "COM1-DataBits": "8", "Vincent-D3-Address": "x", "520 AO-SupportsTriggering": "0", "405-Description": "DA device that is used as a shutter", "640 Shutter-MinVolts": "0.0000", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "405 AO-Name": "AnalogIO", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "640 AO-TriggerInputLine": "/Dev1/PFI0", "405 AO-TriggerInputLine": "/Dev1/PFI0", "640-Description": "DA device that is used as a shutter", "445 Shutter-TriggerSampleRate": "100000", "470 Shutter-TriggerSequenceLength": "1000", "COM1-StopBits": "1", "520 Shutter-SupportsTriggering": "0", "405-State": "0", "Core-Initialize": "1", "XLIGHT Emission Wheel-State": "3", "520 AO-Description": "NI DAC", "528 AO-TriggerInputLine": "/Dev1/PFI0", "445 Shutter-MaxVolts": "5.0000", "COM9-Handshaking": "Off", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "XLIGHT Spin motor-Label": "On (spinning)", "PRIME-PP 5 ENABLED": "No", "UUID": "36ff2d4f-b8c8-4d18-a1e0-d4e5f6e361b9", "PRIME-TriggerMode": "Internal Trigger", "COM1-Handshaking": "Off", "PRIME-Timing-PostTriggerDelayNs": "49344000.0000", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "Focus-Position": "16732.9000", "528 Shutter-Block voltage": "false", "Channel": "Il-A", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "555 Shutter-Demo": "No", "405 Shutter-Demo": "No", "528 AO-Name": "AnalogIO", "520-State": "0", "Core-Galvo": "", "405 Shutter-TriggerSampleRate": "100000", "Focus-FocusStepSize_(nm)": "100", "PRIME-PixelType": "16bit", "COM4-DataBits": "8", "470 AO-IOChannel": "Dev1/ao2", "520 AO-MinVolts": "0.0000", "COM9-Parity": "None", "PRIME-CircularBufferEnabled": "ON", "PRIME-PP 6 Reset": "No", "640 AO-Description": "NI DAC", "Source": "PRIME", "445 AO-MinVolts": "0.0000", "PIEZO-Name": "Stage", "445 AO-Description": "NI DAC", "PRIME-CircularBufferFrameCount": "46", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "520 AO-Volts": "0.0000", "528 Shutter-TriggerSampleRate": "100000", "XPositionUm": 23269.100000000002, "OpticalPath-State": "4", "PRIME-PP 3 THRESHOLD": "80", "PRIME-PVCAM Version": "3.7.1", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Gain": "1-HDR", "640 Shutter-TriggerSequenceLength": "1000", "528 AO-SupportsTriggering": "0", "445 AO-TriggerInputLine": "/Dev1/PFI0", "OpticalPath-Name": "OpticalPath", "PRIME-PP 4 ENABLED": "Yes", "PRIME-PVCAM Adapter Version": "1.3.29", "COM3-Name": "COM3", "405 AO-MaxVolts": "5.0000", "520 Shutter-Block voltage": "false", "PRIME-Timing-ClearingTimeNs": "49344000.0000", "Core-Focus": "PIEZO", "528 AO-Block voltage": "false", "TE2000-Description": "Nikon TE2000 hub", "528 AO-MinVolts": "0.0000", "COM4-Parity": "None", "Summary": { "Slices": 11, "ChContrastMin": [ 0, 0, 0, 0, 0, 0 ], "PixelAspect": 1, "SlicesFirst": true, "Interval_ms": 0, "Time": "2019-12-17 03:59:33 -0500", "Prefix": "seq_7", "InitialPositionList": [ { "Label": "Pos0", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [149.91], "Focus": [16657.7], "XYStage": [ 71629.7, 19152.9 ] }, "GridColumnIndex": 0 }, { "Label": "Pos1", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [190.82], "Focus": [16485.4], "XYStage": [ 65702.3, 19396.600000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos2", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [194.69], "Focus": [16446.8], "XYStage": [ 59880.5, 19007.8 ] }, "GridColumnIndex": 0 }, { "Label": "Pos3", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [145.04], "Focus": [16446.6], "XYStage": [ 53616.9, 18818.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos4", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [169.57500000000002], "Focus": [16446.5], "XYStage": [ 47662.8, 18817.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos5", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [131.805], "Focus": [16446.4], "XYStage": [ 41918.8, 18298.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos6", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [113.145], "Focus": [16446.5], "XYStage": [ 36129.9, 18895.8 ] }, "GridColumnIndex": 0 }, { "Label": "Pos7", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [141.005], "Focus": [16446.6], "XYStage": [ 30299.100000000002, 18895.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos8", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [140.97], "Focus": [16446.5], "XYStage": [ 23952.100000000002, 19156 ] }, "GridColumnIndex": 0 }, { "Label": "Pos9", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [184.57500000000002], "Focus": [16677.9], "XYStage": [ 72866.8, -14809.300000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos10", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [167.95000000000002], "Focus": [16557.9], "XYStage": [ 66515.6, -14371.300000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos11", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [168], "Focus": [16618.9], "XYStage": [ 59724.3, -13964.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos12", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [203.205], "Focus": [16779.6], "XYStage": [ 53862.3, -13494.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos13", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [203.20000000000002], "Focus": [16749], "XYStage": [ 48108.3, -13805.400000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos14", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [203.19], "Focus": [16675.9], "XYStage": [ 41645, -13804.900000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos15", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [203.21], "Focus": [16675.7], "XYStage": [ 35352.6, -13805.6 ] }, "GridColumnIndex": 0 }, { "Label": "Pos16", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [203.225], "Focus": [16675.8], "XYStage": [ 29298, -13447.400000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos17", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [203.215], "Focus": [16675.8], "XYStage": [ 23269.100000000002, -13012.300000000001 ] }, "GridColumnIndex": 0 } ], "z-step_um": 10, "GridRow": 0, "BitDepth": 16, "Depth": 2, "Channels": 6, "Source": "Micro-Manager", "ChColors": [ -16711681, -256, -65281, -1, -1, -16776961 ], "MetadataVersion": 10, "KeepShutterOpenChannels": true, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536, 65536 ], "CustomIntervals_ms": [], "Height": 1200, "MicroManagerVersion": "1.4.23 20180219", "GridColumn": 0, "UUID": "3ad8801b-5699-40d6-813b-4ad840863e38", "PixelType": "GRAY16", "Width": 1200, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\Yan\\20191216-YC113-S11S12-miseq", "Frames": 1, "Comment": "", "UserName": "Nikon", "PixelSize_um": 1.1, "ROI": [ 0, 0, 1200, 1200 ], "Date": "2019-12-17", "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC", "P-DAPI-high" ], "KeepShutterOpenSlices": true, "IJType": 1, "ComputerName": "ZadorConfocal2", "Positions": 18, "PositionIndex": 17, "CameraTimeout": 20000, "TimeFirst": false }, "640 AO-Name": "AnalogIO", "Vincent-D3-Command": "Close" }, "FrameKey-0-2-7": { "COM4-StopBits": "1", "528 AO-MaxVolts": "5.0000", "XLIGHT Excitation Wheel-State": "1", "470 Shutter-Name": "AnalogIO", "Core-Shutter": "640", "555 AO-Demo": "No", "XLIGHT Dichroic-State": "2", "XYStage-StepSize-Y": "0.1000", "ZPositionUm": 223.215, "XYStage-StepSize-X": "0.1000", "PRIME-FirmwareVersion": "20.28", "PRIME-ClearCycles": "2", "520-Name": "DA Shutter", "405 Shutter-Name": "AnalogIO", "PIEZO-StepSize": "0.0050", "PRIME-ClearMode": "Pre-Exposure", "470 AO-MinVolts": "0.0000", "405 AO-SupportsTriggering": "1", "CameraChannelIndex": 0, "XYStage-StartSpeed": "500.0000", "COM9-StopBits": "2", "405 AO-Description": "NI DAC", "COM4-DelayBetweenCharsMs": "0.0000", "445 AO-Block voltage": "false", "520 AO-TriggerInputLine": "/Dev1/PFI0", "555 AO-Description": "NI DAC", "520 AO-TriggerSampleRate": "100000", "COM4-Verbose": "1", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM4-Name": "COM4", "405 Shutter-Volts": "0.0000", "Vincent-D3-Port": "COM1", "PRIME-PP 4 MIN ADU AFFECTED": "200", "470 Shutter-MinVolts": "0.0000", "PRIME-PP 3 ENABLED": "Yes", "470 AO-Description": "NI DAC", "PRIME-Description": "PVCAM API device adapter", "520 Shutter-MaxVolts": "5.0000", "520 Shutter-Volts": "0.0000", "528 AO-Sequenceable": "1", "PRIME-Binning": "1x1", "555 Shutter-MaxVolts": "5.0000", "528 Shutter-TriggerSequenceLength": "1000", "405 Shutter-Block voltage": "false", "XLIGHT Spin motor-State": "1", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "Vincent-D3-Time to open (ms)": "35.0000", "Vincent-D3-Time to close (ms)": "35.0000", "445 AO-TriggerSampleRate": "100000", "640 Shutter-IOChannel": "Dev1/ao14", "555 AO-Sequenceable": "1", "520 AO-Name": "AnalogIO", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "470 AO-Block voltage": "false", "COM1-Verbose": "1", "COM9-AnswerTimeout": "10000.0000", "528 Shutter-Name": "AnalogIO", "PRIME-Trigger Timeout (secs)": "10", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "NextFrame": 0, "470 AO-TriggerSequenceLength": "1000", "PRIME-TransposeXY": "0", "445 AO-TriggerSequenceLength": "1000", "COM1-Name": "COM1", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "COM9-DataBits": "8", "COM1-AnswerTimeout": "500.0000", "Camera": "", "TE2000-Version": "V2.50-V2.50-V2.10", "528 Shutter-Volts": "0.0000", "XLIGHT Hub-Name": "XLIGHT Hub", "PIEZO-Description": "Ludl stage driver adapter", "PositionName": "Pos17", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSampleRate": "100000", "PRIME-FanSpeedSetpoint": "High", "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "405 AO-TriggerSampleRate": "100000", "470 Shutter-Block voltage": "false", "470-State": "0", "COM3-Parity": "None", "405 AO-Volts": "0.0000", "PRIME-TransposeMirrorY": "0", "PRIME-ChipName": "GS144BSI", "470 AO-Volts": "0.0000", "PRIME-TransposeMirrorX": "0", "445 Shutter-SupportsTriggering": "0", "470 Shutter-SupportsTriggering": "0", "XLIGHT Hub-Description": "XLIGHT hub", "PRIME-PreampOffLimit": "10000", "470 AO-Sequenceable": "1", "528 Shutter-MaxVolts": "5.0000", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "Core-XYStage": "XYStage", "470 AO-TriggerSampleRate": "100000", "PRIME-Timing-PreTriggerDelayNs": "24672000.0000", "528-DA Device": "528 Shutter", "Focus-Description": "Nikon TE2000 focus adapter", "FilterBlock-State": "5", "520 AO-Demo": "No", "COM3-DelayBetweenCharsMs": "0.0000", "470 Shutter-Volts": "0.0000", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "PRIME-PP 2 THRESHOLD": "125", "PRIME-PP 4 THRESHOLD": "75", "XYStage-Acceleration": "4.0000", "470 Shutter-Demo": "No", "PRIME-Name": "PMPCIECam00", "640-DA Device": "640 Shutter", "405 AO-Block voltage": "false", "ElapsedTime-ms": 732483, "PRIME-Offset": "100", "445 AO-IOChannel": "Dev1/ao1", "640 AO-MaxVolts": "5.0000", "PositionIndex": 17, "PRIME-PP 1 ENABLED": "Yes", "COM1-BaudRate": "9600", "640-State": "1", "COM3-Verbose": "1", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "LudlController-TransmissionDelay": "0", "PRIME-FullWellCapacity": "62000", "PRIME-SMARTStreamingEnabled": "No", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "640 Shutter-Description": "NI DAC", "528-Description": "DA device that is used as a shutter", "COM4-Description": "Serial port driver (boost:asio)", "405 Shutter-Description": "NI DAC", "TE2000-Name": "TE2000", "528 Shutter-Description": "NI DAC", "COM9-Description": "Serial port driver (boost:asio)", "LudlController-Name": "LudlController", "445 Shutter-TriggerSequenceLength": "1000", "PRIME-SerialNumber": "A18B203008", "445 AO-Volts": "0.0000", "PRIME-X-dimension": "1200", "PRIME-ShutterCloseDelay": "0", "XLIGHT Disk slider-State": "2", "Position": "Default", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 AO-IOChannel": "Dev1/ao5", "520 AO-IOChannel": "Dev1/ao4", "528 AO-TriggerSequenceLength": "1000", "405 Shutter-MaxVolts": "5.0000", "470-DA Device": "470 Shutter", "PRIME-FTCapable": "No", "Core-ChannelGroup": "Channels", "555 AO-SupportsTriggering": "0", "PRIME-Y-dimension": "1200", "Frame": 0, "555 Shutter-Sequenceable": "1", "PRIME-Timing-ExposureTimeNs": "499998000.0000", "Core-ImageProcessor": "", "528-State": "0", "520 AO-Block voltage": "false", "XLIGHT Excitation Wheel-Label": "QUAD", "640 Shutter-Demo": "No", "COM3-StopBits": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "520 Shutter-IOChannel": "Dev1/ao11", "Width": 1200, "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-SupportsTriggering": "0", "640 AO-TriggerSampleRate": "100000", "520-Description": "DA device that is used as a shutter", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "405 Shutter-Sequenceable": "1", "405 Shutter-SupportsTriggering": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-TriggerSampleRate": "100000", "640 Shutter-MaxVolts": "5.0000", "555 AO-MaxVolts": "5.0000", "520 AO-TriggerSequenceLength": "1000", "COM3-BaudRate": "9600", "COM9-BaudRate": "9600", "Vincent-D3-Channel #": "Ch. #1", "PRIME-PMode": "Normal", "OpticalPath-Label": "Left(100)", "BitDepth": 16, "555 AO-TriggerSequenceLength": "1000", "445 Shutter-Description": "NI DAC", "405 AO-Sequenceable": "1", "PRIME-AcquisitionMethod": "Callbacks", "555 AO-Name": "AnalogIO", "PRIME-ShutterMode": "Pre-Exposure", "COM3-Handshaking": "Off", "445 AO-SupportsTriggering": "0", "ROI": "0-0-1200-1200", "COM9-Verbose": "1", "PRIME-Color": "OFF", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "PRIME-ShutterOpenDelay": "0", "COM1-Parity": "None", "Slice": 7, "SlicePosition": 20, "COM9-DelayBetweenCharsMs": "0.0000", "555-Name": "DA Shutter", "PIEZO-LudlSingleAxisName": "B", "XLIGHT Emission Wheel-Label": "679/29 (A)", "640 Shutter-Name": "AnalogIO", "640 AO-Sequenceable": "1", "520 Shutter-Demo": "No", "528 Shutter-Demo": "No", "470-Name": "DA Shutter", "520-DA Device": "520 Shutter", "XYStage-Description": "Ludl XY stage driver adapter", "445 Shutter-MinVolts": "0.0000", "528 AO-TriggerSampleRate": "100000", "405 AO-MinVolts": "0.0000", "405 Shutter-TriggerSequenceLength": "1000", "Height": 1200, "555 AO-Block voltage": "false", "640 Shutter-Volts": "5.0000", "PixelType": "GRAY16", "445 Shutter-Block voltage": "false", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "520 Shutter-TriggerSequenceLength": "1000", "COM9-Name": "COM9", "Core-SLM": "", "555 AO-Volts": "0.0000", "555 Shutter-Block voltage": "false", "XYStage-Speed": "1800.0000", "520 AO-MaxVolts": "5.0000", "528 Shutter-SupportsTriggering": "0", "COM1-Description": "Serial port driver (boost:asio)", "PRIME-MetadataEnabled": "No", "520 Shutter-MinVolts": "0.0000", "470 Shutter-Sequenceable": "1", "PRIME-PreampDelay": "0", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-MinVolts": "0.0000", "ChannelIndex": 2, "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Core-AutoShutter": "1", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "Exposure-ms": 320, "PRIME-PP 2 ENABLED": "Yes", "640 Shutter-TriggerSampleRate": "100000", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "470 Shutter-IOChannel": "Dev1/ao10", "405 AO-Demo": "No", "PixelSizeUm": 1.1, "470 AO-Demo": "No", "470 Shutter-MaxVolts": "5.0000", "Vincent-D3-Name": "Vincent-D3", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FileName": "img_000000000_Il-A_007.tif", "LudlController-Reset": "Operate", "PRIME-Exposure": "320.00", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "445-Name": "DA Shutter", "555 Shutter-Name": "AnalogIO", "520 Shutter-TriggerSampleRate": "100000", "445 Shutter-IOChannel": "Dev1/ao9", "PRIME-TransposeCorrection": "0", "XYStage-StepSize": "0.1000", "445-Description": "DA device that is used as a shutter", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 AO-IOChannel": "Dev1/ao7", "520 Shutter-Name": "AnalogIO", "COM3-DataBits": "8", "640 AO-Volts": "4.5000", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "555 Shutter-IOChannel": "Dev1/ao13", "WaitInterval": null, "520 Shutter-Sequenceable": "1", "YPositionUm": -13012.300000000001, "445-State": "0", "445-DA Device": "445 Shutter", "PRIME-CircularBufferAutoSize": "ON", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "555 AO-TriggerSampleRate": "100000", "445 Shutter-Volts": "0.0000", "XLIGHT Hub-Port": "COM3", "Core-AutoFocus": "", "COM4-Handshaking": "Off", "528 Shutter-MinVolts": "0.0000", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "445 Shutter-Name": "AnalogIO", "528 Shutter-Sequenceable": "1", "XYStage-TransposeMirrorX": "0", "XYStage-TransposeMirrorY": "0", "PRIME-ReadoutRate": "100MHz 16bit", "XYStage-Name": "Stage", "640 AO-SupportsTriggering": "0", "640-Name": "DA Shutter", "640 Shutter-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "640 AO-Block voltage": "false", "445 Shutter-Sequenceable": "1", "470 AO-MaxVolts": "5.0000", "COM4-BaudRate": "9600", "470-Description": "DA device that is used as a shutter", "520 Shutter-Description": "NI DAC", "PRIME-CCDTemperature": "-14.9200", "Time": "2019-12-17 03:59:49 -0500", "640 AO-TriggerSequenceLength": "1000", "470 AO-Name": "AnalogIO", "XLIGHT Disk slider-Label": "Disk Pos 40um", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "Binning": "1x1", "TE2000-ModelType": "1", "COM3-AnswerTimeout": "500.0000", "555 Shutter-SupportsTriggering": "0", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "TE2000-Port": "COM4", "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Description": "NI DAC", "COM4-AnswerTimeout": "500.0000", "COM1-DelayBetweenCharsMs": "0.0000", "FilterBlock-Label": "Confocal", "445 AO-Name": "AnalogIO", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-Timing-ReadoutTimeNs": "24672000.0000", "528 AO-Demo": "No", "520 AO-Sequenceable": "1", "555-Description": "DA device that is used as a shutter", "405-DA Device": "405 Shutter", "405 AO-TriggerSequenceLength": "1000", "640 AO-MinVolts": "0.0000", "640 Shutter-Block voltage": "false", "FrameIndex": 0, "PRIME-Port": "CMOS", "555 AO-MinVolts": "0.0000", "COM3-Description": "Serial port driver (boost:asio)", "528 AO-Description": "NI DAC", "555 AO-IOChannel": "Dev1/ao6", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "555 AO-TriggerInputLine": "/Dev1/PFI0", "PRIME-ActualInterval-ms": "84.6200", "LudlController-Port": "COM9", "445 Shutter-Demo": "No", "PRIME-Trigger-Expose Out-Mux": "1", "SliceIndex": 7, "PRIME-CCDTemperatureSetPoint": "-15.0000", "405 Shutter-MinVolts": "0.0000", "PRIME-ExposeOutMode": "First Row", "AxisPositions": null, "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Volts": "0.0000", "528-Name": "DA Shutter", "555-State": "0", "XLIGHT Dichroic-Label": "Di03-R635-t1", "445 AO-Sequenceable": "1", "405-Name": "DA Shutter", "405 AO-IOChannel": "Dev1/ao0", "Core-Camera": "PRIME", "Focus-Name": "Focus", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "405 Shutter-IOChannel": "Dev1/ao8", "640 AO-Demo": "No", "640 Shutter-SupportsTriggering": "0", "445 AO-Demo": "No", "555-DA Device": "555 Shutter", "470 Shutter-Description": "NI DAC", "528 AO-Volts": "0.0000", "COM1-DataBits": "8", "Vincent-D3-Address": "x", "520 AO-SupportsTriggering": "0", "405-Description": "DA device that is used as a shutter", "640 Shutter-MinVolts": "0.0000", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "405 AO-Name": "AnalogIO", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "640 AO-TriggerInputLine": "/Dev1/PFI0", "405 AO-TriggerInputLine": "/Dev1/PFI0", "640-Description": "DA device that is used as a shutter", "445 Shutter-TriggerSampleRate": "100000", "470 Shutter-TriggerSequenceLength": "1000", "COM1-StopBits": "1", "520 Shutter-SupportsTriggering": "0", "405-State": "0", "Core-Initialize": "1", "XLIGHT Emission Wheel-State": "3", "520 AO-Description": "NI DAC", "528 AO-TriggerInputLine": "/Dev1/PFI0", "445 Shutter-MaxVolts": "5.0000", "COM9-Handshaking": "Off", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "XLIGHT Spin motor-Label": "On (spinning)", "PRIME-PP 5 ENABLED": "No", "UUID": "d382141e-35ca-4668-b0eb-bb208d2d38ad", "PRIME-TriggerMode": "Internal Trigger", "COM1-Handshaking": "Off", "PRIME-Timing-PostTriggerDelayNs": "49344000.0000", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "Focus-Position": "16732.9000", "528 Shutter-Block voltage": "false", "Channel": "Il-A", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "555 Shutter-Demo": "No", "405 Shutter-Demo": "No", "528 AO-Name": "AnalogIO", "520-State": "0", "Core-Galvo": "", "405 Shutter-TriggerSampleRate": "100000", "Focus-FocusStepSize_(nm)": "100", "PRIME-PixelType": "16bit", "COM4-DataBits": "8", "470 AO-IOChannel": "Dev1/ao2", "520 AO-MinVolts": "0.0000", "COM9-Parity": "None", "PRIME-CircularBufferEnabled": "ON", "PRIME-PP 6 Reset": "No", "640 AO-Description": "NI DAC", "Source": "PRIME", "445 AO-MinVolts": "0.0000", "PIEZO-Name": "Stage", "445 AO-Description": "NI DAC", "PRIME-CircularBufferFrameCount": "46", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "520 AO-Volts": "0.0000", "528 Shutter-TriggerSampleRate": "100000", "XPositionUm": 23269.100000000002, "OpticalPath-State": "4", "PRIME-PP 3 THRESHOLD": "80", "PRIME-PVCAM Version": "3.7.1", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Gain": "1-HDR", "640 Shutter-TriggerSequenceLength": "1000", "528 AO-SupportsTriggering": "0", "445 AO-TriggerInputLine": "/Dev1/PFI0", "OpticalPath-Name": "OpticalPath", "PRIME-PP 4 ENABLED": "Yes", "PRIME-PVCAM Adapter Version": "1.3.29", "COM3-Name": "COM3", "405 AO-MaxVolts": "5.0000", "520 Shutter-Block voltage": "false", "PRIME-Timing-ClearingTimeNs": "49344000.0000", "Core-Focus": "PIEZO", "528 AO-Block voltage": "false", "TE2000-Description": "Nikon TE2000 hub", "528 AO-MinVolts": "0.0000", "COM4-Parity": "None", "Summary": { "Slices": 11, "ChContrastMin": [ 0, 0, 0, 0, 0, 0 ], "PixelAspect": 1, "SlicesFirst": true, "Interval_ms": 0, "Time": "2019-12-17 03:59:33 -0500", "Prefix": "seq_7", "InitialPositionList": [ { "Label": "Pos0", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [149.91], "Focus": [16657.7], "XYStage": [ 71629.7, 19152.9 ] }, "GridColumnIndex": 0 }, { "Label": "Pos1", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [190.82], "Focus": [16485.4], "XYStage": [ 65702.3, 19396.600000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos2", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [194.69], "Focus": [16446.8], "XYStage": [ 59880.5, 19007.8 ] }, "GridColumnIndex": 0 }, { "Label": "Pos3", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [145.04], "Focus": [16446.6], "XYStage": [ 53616.9, 18818.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos4", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [169.57500000000002], "Focus": [16446.5], "XYStage": [ 47662.8, 18817.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos5", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [131.805], "Focus": [16446.4], "XYStage": [ 41918.8, 18298.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos6", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [113.145], "Focus": [16446.5], "XYStage": [ 36129.9, 18895.8 ] }, "GridColumnIndex": 0 }, { "Label": "Pos7", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [141.005], "Focus": [16446.6], "XYStage": [ 30299.100000000002, 18895.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos8", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [140.97], "Focus": [16446.5], "XYStage": [ 23952.100000000002, 19156 ] }, "GridColumnIndex": 0 }, { "Label": "Pos9", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [184.57500000000002], "Focus": [16677.9], "XYStage": [ 72866.8, -14809.300000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos10", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [167.95000000000002], "Focus": [16557.9], "XYStage": [ 66515.6, -14371.300000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos11", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [168], "Focus": [16618.9], "XYStage": [ 59724.3, -13964.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos12", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [203.205], "Focus": [16779.6], "XYStage": [ 53862.3, -13494.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos13", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [203.20000000000002], "Focus": [16749], "XYStage": [ 48108.3, -13805.400000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos14", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [203.19], "Focus": [16675.9], "XYStage": [ 41645, -13804.900000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos15", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [203.21], "Focus": [16675.7], "XYStage": [ 35352.6, -13805.6 ] }, "GridColumnIndex": 0 }, { "Label": "Pos16", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [203.225], "Focus": [16675.8], "XYStage": [ 29298, -13447.400000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos17", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [203.215], "Focus": [16675.8], "XYStage": [ 23269.100000000002, -13012.300000000001 ] }, "GridColumnIndex": 0 } ], "z-step_um": 10, "GridRow": 0, "BitDepth": 16, "Depth": 2, "Channels": 6, "Source": "Micro-Manager", "ChColors": [ -16711681, -256, -65281, -1, -1, -16776961 ], "MetadataVersion": 10, "KeepShutterOpenChannels": true, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536, 65536 ], "CustomIntervals_ms": [], "Height": 1200, "MicroManagerVersion": "1.4.23 20180219", "GridColumn": 0, "UUID": "3ad8801b-5699-40d6-813b-4ad840863e38", "PixelType": "GRAY16", "Width": 1200, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\Yan\\20191216-YC113-S11S12-miseq", "Frames": 1, "Comment": "", "UserName": "Nikon", "PixelSize_um": 1.1, "ROI": [ 0, 0, 1200, 1200 ], "Date": "2019-12-17", "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC", "P-DAPI-high" ], "KeepShutterOpenSlices": true, "IJType": 1, "ComputerName": "ZadorConfocal2", "Positions": 18, "PositionIndex": 17, "CameraTimeout": 20000, "TimeFirst": false }, "640 AO-Name": "AnalogIO", "Vincent-D3-Command": "Close" }, "FrameKey-0-2-8": { "COM4-StopBits": "1", "528 AO-MaxVolts": "5.0000", "XLIGHT Excitation Wheel-State": "1", "470 Shutter-Name": "AnalogIO", "Core-Shutter": "640", "555 AO-Demo": "No", "XLIGHT Dichroic-State": "2", "XYStage-StepSize-Y": "0.1000", "ZPositionUm": 233.215, "XYStage-StepSize-X": "0.1000", "PRIME-FirmwareVersion": "20.28", "PRIME-ClearCycles": "2", "520-Name": "DA Shutter", "405 Shutter-Name": "AnalogIO", "PIEZO-StepSize": "0.0050", "PRIME-ClearMode": "Pre-Exposure", "470 AO-MinVolts": "0.0000", "405 AO-SupportsTriggering": "1", "CameraChannelIndex": 0, "XYStage-StartSpeed": "500.0000", "COM9-StopBits": "2", "405 AO-Description": "NI DAC", "COM4-DelayBetweenCharsMs": "0.0000", "445 AO-Block voltage": "false", "520 AO-TriggerInputLine": "/Dev1/PFI0", "555 AO-Description": "NI DAC", "520 AO-TriggerSampleRate": "100000", "COM4-Verbose": "1", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM4-Name": "COM4", "405 Shutter-Volts": "0.0000", "Vincent-D3-Port": "COM1", "PRIME-PP 4 MIN ADU AFFECTED": "200", "470 Shutter-MinVolts": "0.0000", "PRIME-PP 3 ENABLED": "Yes", "470 AO-Description": "NI DAC", "PRIME-Description": "PVCAM API device adapter", "520 Shutter-MaxVolts": "5.0000", "520 Shutter-Volts": "0.0000", "528 AO-Sequenceable": "1", "PRIME-Binning": "1x1", "555 Shutter-MaxVolts": "5.0000", "528 Shutter-TriggerSequenceLength": "1000", "405 Shutter-Block voltage": "false", "XLIGHT Spin motor-State": "1", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "Vincent-D3-Time to open (ms)": "35.0000", "Vincent-D3-Time to close (ms)": "35.0000", "445 AO-TriggerSampleRate": "100000", "640 Shutter-IOChannel": "Dev1/ao14", "555 AO-Sequenceable": "1", "520 AO-Name": "AnalogIO", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "470 AO-Block voltage": "false", "COM1-Verbose": "1", "COM9-AnswerTimeout": "10000.0000", "528 Shutter-Name": "AnalogIO", "PRIME-Trigger Timeout (secs)": "10", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "NextFrame": 0, "470 AO-TriggerSequenceLength": "1000", "PRIME-TransposeXY": "0", "445 AO-TriggerSequenceLength": "1000", "COM1-Name": "COM1", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "COM9-DataBits": "8", "COM1-AnswerTimeout": "500.0000", "Camera": "", "TE2000-Version": "V2.50-V2.50-V2.10", "528 Shutter-Volts": "0.0000", "XLIGHT Hub-Name": "XLIGHT Hub", "PIEZO-Description": "Ludl stage driver adapter", "PositionName": "Pos17", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSampleRate": "100000", "PRIME-FanSpeedSetpoint": "High", "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "405 AO-TriggerSampleRate": "100000", "470 Shutter-Block voltage": "false", "470-State": "0", "COM3-Parity": "None", "405 AO-Volts": "0.0000", "PRIME-TransposeMirrorY": "0", "PRIME-ChipName": "GS144BSI", "470 AO-Volts": "0.0000", "PRIME-TransposeMirrorX": "0", "445 Shutter-SupportsTriggering": "0", "470 Shutter-SupportsTriggering": "0", "XLIGHT Hub-Description": "XLIGHT hub", "PRIME-PreampOffLimit": "10000", "470 AO-Sequenceable": "1", "528 Shutter-MaxVolts": "5.0000", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "Core-XYStage": "XYStage", "470 AO-TriggerSampleRate": "100000", "PRIME-Timing-PreTriggerDelayNs": "24672000.0000", "528-DA Device": "528 Shutter", "Focus-Description": "Nikon TE2000 focus adapter", "FilterBlock-State": "5", "520 AO-Demo": "No", "COM3-DelayBetweenCharsMs": "0.0000", "470 Shutter-Volts": "0.0000", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "PRIME-PP 2 THRESHOLD": "125", "PRIME-PP 4 THRESHOLD": "75", "XYStage-Acceleration": "4.0000", "470 Shutter-Demo": "No", "PRIME-Name": "PMPCIECam00", "640-DA Device": "640 Shutter", "405 AO-Block voltage": "false", "ElapsedTime-ms": 733042, "PRIME-Offset": "100", "445 AO-IOChannel": "Dev1/ao1", "640 AO-MaxVolts": "5.0000", "PositionIndex": 17, "PRIME-PP 1 ENABLED": "Yes", "COM1-BaudRate": "9600", "640-State": "1", "COM3-Verbose": "1", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "LudlController-TransmissionDelay": "0", "PRIME-FullWellCapacity": "62000", "PRIME-SMARTStreamingEnabled": "No", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "640 Shutter-Description": "NI DAC", "528-Description": "DA device that is used as a shutter", "COM4-Description": "Serial port driver (boost:asio)", "405 Shutter-Description": "NI DAC", "TE2000-Name": "TE2000", "528 Shutter-Description": "NI DAC", "COM9-Description": "Serial port driver (boost:asio)", "LudlController-Name": "LudlController", "445 Shutter-TriggerSequenceLength": "1000", "PRIME-SerialNumber": "A18B203008", "445 AO-Volts": "0.0000", "PRIME-X-dimension": "1200", "PRIME-ShutterCloseDelay": "0", "XLIGHT Disk slider-State": "2", "Position": "Default", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 AO-IOChannel": "Dev1/ao5", "520 AO-IOChannel": "Dev1/ao4", "528 AO-TriggerSequenceLength": "1000", "405 Shutter-MaxVolts": "5.0000", "470-DA Device": "470 Shutter", "PRIME-FTCapable": "No", "Core-ChannelGroup": "Channels", "555 AO-SupportsTriggering": "0", "PRIME-Y-dimension": "1200", "Frame": 0, "555 Shutter-Sequenceable": "1", "PRIME-Timing-ExposureTimeNs": "499998000.0000", "Core-ImageProcessor": "", "528-State": "0", "520 AO-Block voltage": "false", "XLIGHT Excitation Wheel-Label": "QUAD", "640 Shutter-Demo": "No", "COM3-StopBits": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "520 Shutter-IOChannel": "Dev1/ao11", "Width": 1200, "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-SupportsTriggering": "0", "640 AO-TriggerSampleRate": "100000", "520-Description": "DA device that is used as a shutter", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "405 Shutter-Sequenceable": "1", "405 Shutter-SupportsTriggering": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-TriggerSampleRate": "100000", "640 Shutter-MaxVolts": "5.0000", "555 AO-MaxVolts": "5.0000", "520 AO-TriggerSequenceLength": "1000", "COM3-BaudRate": "9600", "COM9-BaudRate": "9600", "Vincent-D3-Channel #": "Ch. #1", "PRIME-PMode": "Normal", "OpticalPath-Label": "Left(100)", "BitDepth": 16, "555 AO-TriggerSequenceLength": "1000", "445 Shutter-Description": "NI DAC", "405 AO-Sequenceable": "1", "PRIME-AcquisitionMethod": "Callbacks", "555 AO-Name": "AnalogIO", "PRIME-ShutterMode": "Pre-Exposure", "COM3-Handshaking": "Off", "445 AO-SupportsTriggering": "0", "ROI": "0-0-1200-1200", "COM9-Verbose": "1", "PRIME-Color": "OFF", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "PRIME-ShutterOpenDelay": "0", "COM1-Parity": "None", "Slice": 8, "SlicePosition": 30, "COM9-DelayBetweenCharsMs": "0.0000", "555-Name": "DA Shutter", "PIEZO-LudlSingleAxisName": "B", "XLIGHT Emission Wheel-Label": "679/29 (A)", "640 Shutter-Name": "AnalogIO", "640 AO-Sequenceable": "1", "520 Shutter-Demo": "No", "528 Shutter-Demo": "No", "470-Name": "DA Shutter", "520-DA Device": "520 Shutter", "XYStage-Description": "Ludl XY stage driver adapter", "445 Shutter-MinVolts": "0.0000", "528 AO-TriggerSampleRate": "100000", "405 AO-MinVolts": "0.0000", "405 Shutter-TriggerSequenceLength": "1000", "Height": 1200, "555 AO-Block voltage": "false", "640 Shutter-Volts": "5.0000", "PixelType": "GRAY16", "445 Shutter-Block voltage": "false", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "520 Shutter-TriggerSequenceLength": "1000", "COM9-Name": "COM9", "Core-SLM": "", "555 AO-Volts": "0.0000", "555 Shutter-Block voltage": "false", "XYStage-Speed": "1800.0000", "520 AO-MaxVolts": "5.0000", "528 Shutter-SupportsTriggering": "0", "COM1-Description": "Serial port driver (boost:asio)", "PRIME-MetadataEnabled": "No", "520 Shutter-MinVolts": "0.0000", "470 Shutter-Sequenceable": "1", "PRIME-PreampDelay": "0", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-MinVolts": "0.0000", "ChannelIndex": 2, "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Core-AutoShutter": "1", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "Exposure-ms": 320, "PRIME-PP 2 ENABLED": "Yes", "640 Shutter-TriggerSampleRate": "100000", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "470 Shutter-IOChannel": "Dev1/ao10", "405 AO-Demo": "No", "PixelSizeUm": 1.1, "470 AO-Demo": "No", "470 Shutter-MaxVolts": "5.0000", "Vincent-D3-Name": "Vincent-D3", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FileName": "img_000000000_Il-A_008.tif", "LudlController-Reset": "Operate", "PRIME-Exposure": "320.00", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "445-Name": "DA Shutter", "555 Shutter-Name": "AnalogIO", "520 Shutter-TriggerSampleRate": "100000", "445 Shutter-IOChannel": "Dev1/ao9", "PRIME-TransposeCorrection": "0", "XYStage-StepSize": "0.1000", "445-Description": "DA device that is used as a shutter", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 AO-IOChannel": "Dev1/ao7", "520 Shutter-Name": "AnalogIO", "COM3-DataBits": "8", "640 AO-Volts": "4.5000", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "555 Shutter-IOChannel": "Dev1/ao13", "WaitInterval": null, "520 Shutter-Sequenceable": "1", "YPositionUm": -13012.300000000001, "445-State": "0", "445-DA Device": "445 Shutter", "PRIME-CircularBufferAutoSize": "ON", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "555 AO-TriggerSampleRate": "100000", "445 Shutter-Volts": "0.0000", "XLIGHT Hub-Port": "COM3", "Core-AutoFocus": "", "COM4-Handshaking": "Off", "528 Shutter-MinVolts": "0.0000", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "445 Shutter-Name": "AnalogIO", "528 Shutter-Sequenceable": "1", "XYStage-TransposeMirrorX": "0", "XYStage-TransposeMirrorY": "0", "PRIME-ReadoutRate": "100MHz 16bit", "XYStage-Name": "Stage", "640 AO-SupportsTriggering": "0", "640-Name": "DA Shutter", "640 Shutter-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "640 AO-Block voltage": "false", "445 Shutter-Sequenceable": "1", "470 AO-MaxVolts": "5.0000", "COM4-BaudRate": "9600", "470-Description": "DA device that is used as a shutter", "520 Shutter-Description": "NI DAC", "PRIME-CCDTemperature": "-14.9200", "Time": "2019-12-17 03:59:50 -0500", "640 AO-TriggerSequenceLength": "1000", "470 AO-Name": "AnalogIO", "XLIGHT Disk slider-Label": "Disk Pos 40um", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "Binning": "1x1", "TE2000-ModelType": "1", "COM3-AnswerTimeout": "500.0000", "555 Shutter-SupportsTriggering": "0", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "TE2000-Port": "COM4", "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Description": "NI DAC", "COM4-AnswerTimeout": "500.0000", "COM1-DelayBetweenCharsMs": "0.0000", "FilterBlock-Label": "Confocal", "445 AO-Name": "AnalogIO", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-Timing-ReadoutTimeNs": "24672000.0000", "528 AO-Demo": "No", "520 AO-Sequenceable": "1", "555-Description": "DA device that is used as a shutter", "405-DA Device": "405 Shutter", "405 AO-TriggerSequenceLength": "1000", "640 AO-MinVolts": "0.0000", "640 Shutter-Block voltage": "false", "FrameIndex": 0, "PRIME-Port": "CMOS", "555 AO-MinVolts": "0.0000", "COM3-Description": "Serial port driver (boost:asio)", "528 AO-Description": "NI DAC", "555 AO-IOChannel": "Dev1/ao6", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "555 AO-TriggerInputLine": "/Dev1/PFI0", "PRIME-ActualInterval-ms": "84.6200", "LudlController-Port": "COM9", "445 Shutter-Demo": "No", "PRIME-Trigger-Expose Out-Mux": "1", "SliceIndex": 8, "PRIME-CCDTemperatureSetPoint": "-15.0000", "405 Shutter-MinVolts": "0.0000", "PRIME-ExposeOutMode": "First Row", "AxisPositions": null, "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Volts": "0.0000", "528-Name": "DA Shutter", "555-State": "0", "XLIGHT Dichroic-Label": "Di03-R635-t1", "445 AO-Sequenceable": "1", "405-Name": "DA Shutter", "405 AO-IOChannel": "Dev1/ao0", "Core-Camera": "PRIME", "Focus-Name": "Focus", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "405 Shutter-IOChannel": "Dev1/ao8", "640 AO-Demo": "No", "640 Shutter-SupportsTriggering": "0", "445 AO-Demo": "No", "555-DA Device": "555 Shutter", "470 Shutter-Description": "NI DAC", "528 AO-Volts": "0.0000", "COM1-DataBits": "8", "Vincent-D3-Address": "x", "520 AO-SupportsTriggering": "0", "405-Description": "DA device that is used as a shutter", "640 Shutter-MinVolts": "0.0000", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "405 AO-Name": "AnalogIO", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "640 AO-TriggerInputLine": "/Dev1/PFI0", "405 AO-TriggerInputLine": "/Dev1/PFI0", "640-Description": "DA device that is used as a shutter", "445 Shutter-TriggerSampleRate": "100000", "470 Shutter-TriggerSequenceLength": "1000", "COM1-StopBits": "1", "520 Shutter-SupportsTriggering": "0", "405-State": "0", "Core-Initialize": "1", "XLIGHT Emission Wheel-State": "3", "520 AO-Description": "NI DAC", "528 AO-TriggerInputLine": "/Dev1/PFI0", "445 Shutter-MaxVolts": "5.0000", "COM9-Handshaking": "Off", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "XLIGHT Spin motor-Label": "On (spinning)", "PRIME-PP 5 ENABLED": "No", "UUID": "0a4f2cd0-da44-469e-bb75-0e913a7f3794", "PRIME-TriggerMode": "Internal Trigger", "COM1-Handshaking": "Off", "PRIME-Timing-PostTriggerDelayNs": "49344000.0000", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "Focus-Position": "16732.9000", "528 Shutter-Block voltage": "false", "Channel": "Il-A", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "555 Shutter-Demo": "No", "405 Shutter-Demo": "No", "528 AO-Name": "AnalogIO", "520-State": "0", "Core-Galvo": "", "405 Shutter-TriggerSampleRate": "100000", "Focus-FocusStepSize_(nm)": "100", "PRIME-PixelType": "16bit", "COM4-DataBits": "8", "470 AO-IOChannel": "Dev1/ao2", "520 AO-MinVolts": "0.0000", "COM9-Parity": "None", "PRIME-CircularBufferEnabled": "ON", "PRIME-PP 6 Reset": "No", "640 AO-Description": "NI DAC", "Source": "PRIME", "445 AO-MinVolts": "0.0000", "PIEZO-Name": "Stage", "445 AO-Description": "NI DAC", "PRIME-CircularBufferFrameCount": "46", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "520 AO-Volts": "0.0000", "528 Shutter-TriggerSampleRate": "100000", "XPositionUm": 23269.100000000002, "OpticalPath-State": "4", "PRIME-PP 3 THRESHOLD": "80", "PRIME-PVCAM Version": "3.7.1", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Gain": "1-HDR", "640 Shutter-TriggerSequenceLength": "1000", "528 AO-SupportsTriggering": "0", "445 AO-TriggerInputLine": "/Dev1/PFI0", "OpticalPath-Name": "OpticalPath", "PRIME-PP 4 ENABLED": "Yes", "PRIME-PVCAM Adapter Version": "1.3.29", "COM3-Name": "COM3", "405 AO-MaxVolts": "5.0000", "520 Shutter-Block voltage": "false", "PRIME-Timing-ClearingTimeNs": "49344000.0000", "Core-Focus": "PIEZO", "528 AO-Block voltage": "false", "TE2000-Description": "Nikon TE2000 hub", "528 AO-MinVolts": "0.0000", "COM4-Parity": "None", "Summary": { "Slices": 11, "ChContrastMin": [ 0, 0, 0, 0, 0, 0 ], "PixelAspect": 1, "SlicesFirst": true, "Interval_ms": 0, "Time": "2019-12-17 03:59:33 -0500", "Prefix": "seq_7", "InitialPositionList": [ { "Label": "Pos0", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [149.91], "Focus": [16657.7], "XYStage": [ 71629.7, 19152.9 ] }, "GridColumnIndex": 0 }, { "Label": "Pos1", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [190.82], "Focus": [16485.4], "XYStage": [ 65702.3, 19396.600000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos2", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [194.69], "Focus": [16446.8], "XYStage": [ 59880.5, 19007.8 ] }, "GridColumnIndex": 0 }, { "Label": "Pos3", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [145.04], "Focus": [16446.6], "XYStage": [ 53616.9, 18818.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos4", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [169.57500000000002], "Focus": [16446.5], "XYStage": [ 47662.8, 18817.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos5", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [131.805], "Focus": [16446.4], "XYStage": [ 41918.8, 18298.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos6", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [113.145], "Focus": [16446.5], "XYStage": [ 36129.9, 18895.8 ] }, "GridColumnIndex": 0 }, { "Label": "Pos7", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [141.005], "Focus": [16446.6], "XYStage": [ 30299.100000000002, 18895.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos8", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [140.97], "Focus": [16446.5], "XYStage": [ 23952.100000000002, 19156 ] }, "GridColumnIndex": 0 }, { "Label": "Pos9", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [184.57500000000002], "Focus": [16677.9], "XYStage": [ 72866.8, -14809.300000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos10", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [167.95000000000002], "Focus": [16557.9], "XYStage": [ 66515.6, -14371.300000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos11", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [168], "Focus": [16618.9], "XYStage": [ 59724.3, -13964.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos12", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [203.205], "Focus": [16779.6], "XYStage": [ 53862.3, -13494.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos13", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [203.20000000000002], "Focus": [16749], "XYStage": [ 48108.3, -13805.400000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos14", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [203.19], "Focus": [16675.9], "XYStage": [ 41645, -13804.900000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos15", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [203.21], "Focus": [16675.7], "XYStage": [ 35352.6, -13805.6 ] }, "GridColumnIndex": 0 }, { "Label": "Pos16", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [203.225], "Focus": [16675.8], "XYStage": [ 29298, -13447.400000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos17", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [203.215], "Focus": [16675.8], "XYStage": [ 23269.100000000002, -13012.300000000001 ] }, "GridColumnIndex": 0 } ], "z-step_um": 10, "GridRow": 0, "BitDepth": 16, "Depth": 2, "Channels": 6, "Source": "Micro-Manager", "ChColors": [ -16711681, -256, -65281, -1, -1, -16776961 ], "MetadataVersion": 10, "KeepShutterOpenChannels": true, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536, 65536 ], "CustomIntervals_ms": [], "Height": 1200, "MicroManagerVersion": "1.4.23 20180219", "GridColumn": 0, "UUID": "3ad8801b-5699-40d6-813b-4ad840863e38", "PixelType": "GRAY16", "Width": 1200, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\Yan\\20191216-YC113-S11S12-miseq", "Frames": 1, "Comment": "", "UserName": "Nikon", "PixelSize_um": 1.1, "ROI": [ 0, 0, 1200, 1200 ], "Date": "2019-12-17", "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC", "P-DAPI-high" ], "KeepShutterOpenSlices": true, "IJType": 1, "ComputerName": "ZadorConfocal2", "Positions": 18, "PositionIndex": 17, "CameraTimeout": 20000, "TimeFirst": false }, "640 AO-Name": "AnalogIO", "Vincent-D3-Command": "Close" }, "FrameKey-0-2-9": { "COM4-StopBits": "1", "528 AO-MaxVolts": "5.0000", "XLIGHT Excitation Wheel-State": "1", "470 Shutter-Name": "AnalogIO", "Core-Shutter": "640", "555 AO-Demo": "No", "XLIGHT Dichroic-State": "2", "XYStage-StepSize-Y": "0.1000", "ZPositionUm": 243.215, "XYStage-StepSize-X": "0.1000", "PRIME-FirmwareVersion": "20.28", "PRIME-ClearCycles": "2", "520-Name": "DA Shutter", "405 Shutter-Name": "AnalogIO", "PIEZO-StepSize": "0.0050", "PRIME-ClearMode": "Pre-Exposure", "470 AO-MinVolts": "0.0000", "405 AO-SupportsTriggering": "1", "CameraChannelIndex": 0, "XYStage-StartSpeed": "500.0000", "COM9-StopBits": "2", "405 AO-Description": "NI DAC", "COM4-DelayBetweenCharsMs": "0.0000", "445 AO-Block voltage": "false", "520 AO-TriggerInputLine": "/Dev1/PFI0", "555 AO-Description": "NI DAC", "520 AO-TriggerSampleRate": "100000", "COM4-Verbose": "1", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM4-Name": "COM4", "405 Shutter-Volts": "0.0000", "Vincent-D3-Port": "COM1", "PRIME-PP 4 MIN ADU AFFECTED": "200", "470 Shutter-MinVolts": "0.0000", "PRIME-PP 3 ENABLED": "Yes", "470 AO-Description": "NI DAC", "PRIME-Description": "PVCAM API device adapter", "520 Shutter-MaxVolts": "5.0000", "520 Shutter-Volts": "0.0000", "528 AO-Sequenceable": "1", "PRIME-Binning": "1x1", "555 Shutter-MaxVolts": "5.0000", "528 Shutter-TriggerSequenceLength": "1000", "405 Shutter-Block voltage": "false", "XLIGHT Spin motor-State": "1", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "Vincent-D3-Time to open (ms)": "35.0000", "Vincent-D3-Time to close (ms)": "35.0000", "445 AO-TriggerSampleRate": "100000", "640 Shutter-IOChannel": "Dev1/ao14", "555 AO-Sequenceable": "1", "520 AO-Name": "AnalogIO", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "470 AO-Block voltage": "false", "COM1-Verbose": "1", "COM9-AnswerTimeout": "10000.0000", "528 Shutter-Name": "AnalogIO", "PRIME-Trigger Timeout (secs)": "10", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "NextFrame": 0, "470 AO-TriggerSequenceLength": "1000", "PRIME-TransposeXY": "0", "445 AO-TriggerSequenceLength": "1000", "COM1-Name": "COM1", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "COM9-DataBits": "8", "COM1-AnswerTimeout": "500.0000", "Camera": "", "TE2000-Version": "V2.50-V2.50-V2.10", "528 Shutter-Volts": "0.0000", "XLIGHT Hub-Name": "XLIGHT Hub", "PIEZO-Description": "Ludl stage driver adapter", "PositionName": "Pos17", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSampleRate": "100000", "PRIME-FanSpeedSetpoint": "High", "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "405 AO-TriggerSampleRate": "100000", "470 Shutter-Block voltage": "false", "470-State": "0", "COM3-Parity": "None", "405 AO-Volts": "0.0000", "PRIME-TransposeMirrorY": "0", "PRIME-ChipName": "GS144BSI", "470 AO-Volts": "0.0000", "PRIME-TransposeMirrorX": "0", "445 Shutter-SupportsTriggering": "0", "470 Shutter-SupportsTriggering": "0", "XLIGHT Hub-Description": "XLIGHT hub", "PRIME-PreampOffLimit": "10000", "470 AO-Sequenceable": "1", "528 Shutter-MaxVolts": "5.0000", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "Core-XYStage": "XYStage", "470 AO-TriggerSampleRate": "100000", "PRIME-Timing-PreTriggerDelayNs": "24672000.0000", "528-DA Device": "528 Shutter", "Focus-Description": "Nikon TE2000 focus adapter", "FilterBlock-State": "5", "520 AO-Demo": "No", "COM3-DelayBetweenCharsMs": "0.0000", "470 Shutter-Volts": "0.0000", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "PRIME-PP 2 THRESHOLD": "125", "PRIME-PP 4 THRESHOLD": "75", "XYStage-Acceleration": "4.0000", "470 Shutter-Demo": "No", "PRIME-Name": "PMPCIECam00", "640-DA Device": "640 Shutter", "405 AO-Block voltage": "false", "ElapsedTime-ms": 733601, "PRIME-Offset": "100", "445 AO-IOChannel": "Dev1/ao1", "640 AO-MaxVolts": "5.0000", "PositionIndex": 17, "PRIME-PP 1 ENABLED": "Yes", "COM1-BaudRate": "9600", "640-State": "1", "COM3-Verbose": "1", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "LudlController-TransmissionDelay": "0", "PRIME-FullWellCapacity": "62000", "PRIME-SMARTStreamingEnabled": "No", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "640 Shutter-Description": "NI DAC", "528-Description": "DA device that is used as a shutter", "COM4-Description": "Serial port driver (boost:asio)", "405 Shutter-Description": "NI DAC", "TE2000-Name": "TE2000", "528 Shutter-Description": "NI DAC", "COM9-Description": "Serial port driver (boost:asio)", "LudlController-Name": "LudlController", "445 Shutter-TriggerSequenceLength": "1000", "PRIME-SerialNumber": "A18B203008", "445 AO-Volts": "0.0000", "PRIME-X-dimension": "1200", "PRIME-ShutterCloseDelay": "0", "XLIGHT Disk slider-State": "2", "Position": "Default", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 AO-IOChannel": "Dev1/ao5", "520 AO-IOChannel": "Dev1/ao4", "528 AO-TriggerSequenceLength": "1000", "405 Shutter-MaxVolts": "5.0000", "470-DA Device": "470 Shutter", "PRIME-FTCapable": "No", "Core-ChannelGroup": "Channels", "555 AO-SupportsTriggering": "0", "PRIME-Y-dimension": "1200", "Frame": 0, "555 Shutter-Sequenceable": "1", "PRIME-Timing-ExposureTimeNs": "499998000.0000", "Core-ImageProcessor": "", "528-State": "0", "520 AO-Block voltage": "false", "XLIGHT Excitation Wheel-Label": "QUAD", "640 Shutter-Demo": "No", "COM3-StopBits": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "520 Shutter-IOChannel": "Dev1/ao11", "Width": 1200, "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-SupportsTriggering": "0", "640 AO-TriggerSampleRate": "100000", "520-Description": "DA device that is used as a shutter", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "405 Shutter-Sequenceable": "1", "405 Shutter-SupportsTriggering": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-TriggerSampleRate": "100000", "640 Shutter-MaxVolts": "5.0000", "555 AO-MaxVolts": "5.0000", "520 AO-TriggerSequenceLength": "1000", "COM3-BaudRate": "9600", "COM9-BaudRate": "9600", "Vincent-D3-Channel #": "Ch. #1", "PRIME-PMode": "Normal", "OpticalPath-Label": "Left(100)", "BitDepth": 16, "555 AO-TriggerSequenceLength": "1000", "445 Shutter-Description": "NI DAC", "405 AO-Sequenceable": "1", "PRIME-AcquisitionMethod": "Callbacks", "555 AO-Name": "AnalogIO", "PRIME-ShutterMode": "Pre-Exposure", "COM3-Handshaking": "Off", "445 AO-SupportsTriggering": "0", "ROI": "0-0-1200-1200", "COM9-Verbose": "1", "PRIME-Color": "OFF", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "PRIME-ShutterOpenDelay": "0", "COM1-Parity": "None", "Slice": 9, "SlicePosition": 40, "COM9-DelayBetweenCharsMs": "0.0000", "555-Name": "DA Shutter", "PIEZO-LudlSingleAxisName": "B", "XLIGHT Emission Wheel-Label": "679/29 (A)", "640 Shutter-Name": "AnalogIO", "640 AO-Sequenceable": "1", "520 Shutter-Demo": "No", "528 Shutter-Demo": "No", "470-Name": "DA Shutter", "520-DA Device": "520 Shutter", "XYStage-Description": "Ludl XY stage driver adapter", "445 Shutter-MinVolts": "0.0000", "528 AO-TriggerSampleRate": "100000", "405 AO-MinVolts": "0.0000", "405 Shutter-TriggerSequenceLength": "1000", "Height": 1200, "555 AO-Block voltage": "false", "640 Shutter-Volts": "5.0000", "PixelType": "GRAY16", "445 Shutter-Block voltage": "false", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "520 Shutter-TriggerSequenceLength": "1000", "COM9-Name": "COM9", "Core-SLM": "", "555 AO-Volts": "0.0000", "555 Shutter-Block voltage": "false", "XYStage-Speed": "1800.0000", "520 AO-MaxVolts": "5.0000", "528 Shutter-SupportsTriggering": "0", "COM1-Description": "Serial port driver (boost:asio)", "PRIME-MetadataEnabled": "No", "520 Shutter-MinVolts": "0.0000", "470 Shutter-Sequenceable": "1", "PRIME-PreampDelay": "0", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-MinVolts": "0.0000", "ChannelIndex": 2, "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Core-AutoShutter": "1", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "Exposure-ms": 320, "PRIME-PP 2 ENABLED": "Yes", "640 Shutter-TriggerSampleRate": "100000", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "470 Shutter-IOChannel": "Dev1/ao10", "405 AO-Demo": "No", "PixelSizeUm": 1.1, "470 AO-Demo": "No", "470 Shutter-MaxVolts": "5.0000", "Vincent-D3-Name": "Vincent-D3", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FileName": "img_000000000_Il-A_009.tif", "LudlController-Reset": "Operate", "PRIME-Exposure": "320.00", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "445-Name": "DA Shutter", "555 Shutter-Name": "AnalogIO", "520 Shutter-TriggerSampleRate": "100000", "445 Shutter-IOChannel": "Dev1/ao9", "PRIME-TransposeCorrection": "0", "XYStage-StepSize": "0.1000", "445-Description": "DA device that is used as a shutter", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 AO-IOChannel": "Dev1/ao7", "520 Shutter-Name": "AnalogIO", "COM3-DataBits": "8", "640 AO-Volts": "4.5000", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "555 Shutter-IOChannel": "Dev1/ao13", "WaitInterval": null, "520 Shutter-Sequenceable": "1", "YPositionUm": -13012.300000000001, "445-State": "0", "445-DA Device": "445 Shutter", "PRIME-CircularBufferAutoSize": "ON", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "555 AO-TriggerSampleRate": "100000", "445 Shutter-Volts": "0.0000", "XLIGHT Hub-Port": "COM3", "Core-AutoFocus": "", "COM4-Handshaking": "Off", "528 Shutter-MinVolts": "0.0000", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "445 Shutter-Name": "AnalogIO", "528 Shutter-Sequenceable": "1", "XYStage-TransposeMirrorX": "0", "XYStage-TransposeMirrorY": "0", "PRIME-ReadoutRate": "100MHz 16bit", "XYStage-Name": "Stage", "640 AO-SupportsTriggering": "0", "640-Name": "DA Shutter", "640 Shutter-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "640 AO-Block voltage": "false", "445 Shutter-Sequenceable": "1", "470 AO-MaxVolts": "5.0000", "COM4-BaudRate": "9600", "470-Description": "DA device that is used as a shutter", "520 Shutter-Description": "NI DAC", "PRIME-CCDTemperature": "-14.9200", "Time": "2019-12-17 03:59:50 -0500", "640 AO-TriggerSequenceLength": "1000", "470 AO-Name": "AnalogIO", "XLIGHT Disk slider-Label": "Disk Pos 40um", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "Binning": "1x1", "TE2000-ModelType": "1", "COM3-AnswerTimeout": "500.0000", "555 Shutter-SupportsTriggering": "0", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "TE2000-Port": "COM4", "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Description": "NI DAC", "COM4-AnswerTimeout": "500.0000", "COM1-DelayBetweenCharsMs": "0.0000", "FilterBlock-Label": "Confocal", "445 AO-Name": "AnalogIO", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-Timing-ReadoutTimeNs": "24672000.0000", "528 AO-Demo": "No", "520 AO-Sequenceable": "1", "555-Description": "DA device that is used as a shutter", "405-DA Device": "405 Shutter", "405 AO-TriggerSequenceLength": "1000", "640 AO-MinVolts": "0.0000", "640 Shutter-Block voltage": "false", "FrameIndex": 0, "PRIME-Port": "CMOS", "555 AO-MinVolts": "0.0000", "COM3-Description": "Serial port driver (boost:asio)", "528 AO-Description": "NI DAC", "555 AO-IOChannel": "Dev1/ao6", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "555 AO-TriggerInputLine": "/Dev1/PFI0", "PRIME-ActualInterval-ms": "84.6200", "LudlController-Port": "COM9", "445 Shutter-Demo": "No", "PRIME-Trigger-Expose Out-Mux": "1", "SliceIndex": 9, "PRIME-CCDTemperatureSetPoint": "-15.0000", "405 Shutter-MinVolts": "0.0000", "PRIME-ExposeOutMode": "First Row", "AxisPositions": null, "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Volts": "0.0000", "528-Name": "DA Shutter", "555-State": "0", "XLIGHT Dichroic-Label": "Di03-R635-t1", "445 AO-Sequenceable": "1", "405-Name": "DA Shutter", "405 AO-IOChannel": "Dev1/ao0", "Core-Camera": "PRIME", "Focus-Name": "Focus", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "405 Shutter-IOChannel": "Dev1/ao8", "640 AO-Demo": "No", "640 Shutter-SupportsTriggering": "0", "445 AO-Demo": "No", "555-DA Device": "555 Shutter", "470 Shutter-Description": "NI DAC", "528 AO-Volts": "0.0000", "COM1-DataBits": "8", "Vincent-D3-Address": "x", "520 AO-SupportsTriggering": "0", "405-Description": "DA device that is used as a shutter", "640 Shutter-MinVolts": "0.0000", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "405 AO-Name": "AnalogIO", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "640 AO-TriggerInputLine": "/Dev1/PFI0", "405 AO-TriggerInputLine": "/Dev1/PFI0", "640-Description": "DA device that is used as a shutter", "445 Shutter-TriggerSampleRate": "100000", "470 Shutter-TriggerSequenceLength": "1000", "COM1-StopBits": "1", "520 Shutter-SupportsTriggering": "0", "405-State": "0", "Core-Initialize": "1", "XLIGHT Emission Wheel-State": "3", "520 AO-Description": "NI DAC", "528 AO-TriggerInputLine": "/Dev1/PFI0", "445 Shutter-MaxVolts": "5.0000", "COM9-Handshaking": "Off", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "XLIGHT Spin motor-Label": "On (spinning)", "PRIME-PP 5 ENABLED": "No", "UUID": "1b446ca4-1bf4-4466-91c4-4f56cc9c025c", "PRIME-TriggerMode": "Internal Trigger", "COM1-Handshaking": "Off", "PRIME-Timing-PostTriggerDelayNs": "49344000.0000", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "Focus-Position": "16732.9000", "528 Shutter-Block voltage": "false", "Channel": "Il-A", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "555 Shutter-Demo": "No", "405 Shutter-Demo": "No", "528 AO-Name": "AnalogIO", "520-State": "0", "Core-Galvo": "", "405 Shutter-TriggerSampleRate": "100000", "Focus-FocusStepSize_(nm)": "100", "PRIME-PixelType": "16bit", "COM4-DataBits": "8", "470 AO-IOChannel": "Dev1/ao2", "520 AO-MinVolts": "0.0000", "COM9-Parity": "None", "PRIME-CircularBufferEnabled": "ON", "PRIME-PP 6 Reset": "No", "640 AO-Description": "NI DAC", "Source": "PRIME", "445 AO-MinVolts": "0.0000", "PIEZO-Name": "Stage", "445 AO-Description": "NI DAC", "PRIME-CircularBufferFrameCount": "46", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "520 AO-Volts": "0.0000", "528 Shutter-TriggerSampleRate": "100000", "XPositionUm": 23269.100000000002, "OpticalPath-State": "4", "PRIME-PP 3 THRESHOLD": "80", "PRIME-PVCAM Version": "3.7.1", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Gain": "1-HDR", "640 Shutter-TriggerSequenceLength": "1000", "528 AO-SupportsTriggering": "0", "445 AO-TriggerInputLine": "/Dev1/PFI0", "OpticalPath-Name": "OpticalPath", "PRIME-PP 4 ENABLED": "Yes", "PRIME-PVCAM Adapter Version": "1.3.29", "COM3-Name": "COM3", "405 AO-MaxVolts": "5.0000", "520 Shutter-Block voltage": "false", "PRIME-Timing-ClearingTimeNs": "49344000.0000", "Core-Focus": "PIEZO", "528 AO-Block voltage": "false", "TE2000-Description": "Nikon TE2000 hub", "528 AO-MinVolts": "0.0000", "COM4-Parity": "None", "Summary": { "Slices": 11, "ChContrastMin": [ 0, 0, 0, 0, 0, 0 ], "PixelAspect": 1, "SlicesFirst": true, "Interval_ms": 0, "Time": "2019-12-17 03:59:33 -0500", "Prefix": "seq_7", "InitialPositionList": [ { "Label": "Pos0", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [149.91], "Focus": [16657.7], "XYStage": [ 71629.7, 19152.9 ] }, "GridColumnIndex": 0 }, { "Label": "Pos1", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [190.82], "Focus": [16485.4], "XYStage": [ 65702.3, 19396.600000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos2", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [194.69], "Focus": [16446.8], "XYStage": [ 59880.5, 19007.8 ] }, "GridColumnIndex": 0 }, { "Label": "Pos3", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [145.04], "Focus": [16446.6], "XYStage": [ 53616.9, 18818.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos4", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [169.57500000000002], "Focus": [16446.5], "XYStage": [ 47662.8, 18817.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos5", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [131.805], "Focus": [16446.4], "XYStage": [ 41918.8, 18298.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos6", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [113.145], "Focus": [16446.5], "XYStage": [ 36129.9, 18895.8 ] }, "GridColumnIndex": 0 }, { "Label": "Pos7", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [141.005], "Focus": [16446.6], "XYStage": [ 30299.100000000002, 18895.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos8", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [140.97], "Focus": [16446.5], "XYStage": [ 23952.100000000002, 19156 ] }, "GridColumnIndex": 0 }, { "Label": "Pos9", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [184.57500000000002], "Focus": [16677.9], "XYStage": [ 72866.8, -14809.300000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos10", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [167.95000000000002], "Focus": [16557.9], "XYStage": [ 66515.6, -14371.300000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos11", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [168], "Focus": [16618.9], "XYStage": [ 59724.3, -13964.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos12", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [203.205], "Focus": [16779.6], "XYStage": [ 53862.3, -13494.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos13", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [203.20000000000002], "Focus": [16749], "XYStage": [ 48108.3, -13805.400000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos14", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [203.19], "Focus": [16675.9], "XYStage": [ 41645, -13804.900000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos15", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [203.21], "Focus": [16675.7], "XYStage": [ 35352.6, -13805.6 ] }, "GridColumnIndex": 0 }, { "Label": "Pos16", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [203.225], "Focus": [16675.8], "XYStage": [ 29298, -13447.400000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos17", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [203.215], "Focus": [16675.8], "XYStage": [ 23269.100000000002, -13012.300000000001 ] }, "GridColumnIndex": 0 } ], "z-step_um": 10, "GridRow": 0, "BitDepth": 16, "Depth": 2, "Channels": 6, "Source": "Micro-Manager", "ChColors": [ -16711681, -256, -65281, -1, -1, -16776961 ], "MetadataVersion": 10, "KeepShutterOpenChannels": true, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536, 65536 ], "CustomIntervals_ms": [], "Height": 1200, "MicroManagerVersion": "1.4.23 20180219", "GridColumn": 0, "UUID": "3ad8801b-5699-40d6-813b-4ad840863e38", "PixelType": "GRAY16", "Width": 1200, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\Yan\\20191216-YC113-S11S12-miseq", "Frames": 1, "Comment": "", "UserName": "Nikon", "PixelSize_um": 1.1, "ROI": [ 0, 0, 1200, 1200 ], "Date": "2019-12-17", "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC", "P-DAPI-high" ], "KeepShutterOpenSlices": true, "IJType": 1, "ComputerName": "ZadorConfocal2", "Positions": 18, "PositionIndex": 17, "CameraTimeout": 20000, "TimeFirst": false }, "640 AO-Name": "AnalogIO", "Vincent-D3-Command": "Close" }, "FrameKey-0-2-10": { "COM4-StopBits": "1", "528 AO-MaxVolts": "5.0000", "XLIGHT Excitation Wheel-State": "1", "470 Shutter-Name": "AnalogIO", "Core-Shutter": "640", "555 AO-Demo": "No", "XLIGHT Dichroic-State": "2", "XYStage-StepSize-Y": "0.1000", "ZPositionUm": 253.215, "XYStage-StepSize-X": "0.1000", "PRIME-FirmwareVersion": "20.28", "PRIME-ClearCycles": "2", "520-Name": "DA Shutter", "405 Shutter-Name": "AnalogIO", "PIEZO-StepSize": "0.0050", "PRIME-ClearMode": "Pre-Exposure", "470 AO-MinVolts": "0.0000", "405 AO-SupportsTriggering": "1", "CameraChannelIndex": 0, "XYStage-StartSpeed": "500.0000", "COM9-StopBits": "2", "405 AO-Description": "NI DAC", "COM4-DelayBetweenCharsMs": "0.0000", "445 AO-Block voltage": "false", "520 AO-TriggerInputLine": "/Dev1/PFI0", "555 AO-Description": "NI DAC", "520 AO-TriggerSampleRate": "100000", "COM4-Verbose": "1", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM4-Name": "COM4", "405 Shutter-Volts": "0.0000", "Vincent-D3-Port": "COM1", "PRIME-PP 4 MIN ADU AFFECTED": "200", "470 Shutter-MinVolts": "0.0000", "PRIME-PP 3 ENABLED": "Yes", "470 AO-Description": "NI DAC", "PRIME-Description": "PVCAM API device adapter", "520 Shutter-MaxVolts": "5.0000", "520 Shutter-Volts": "0.0000", "528 AO-Sequenceable": "1", "PRIME-Binning": "1x1", "555 Shutter-MaxVolts": "5.0000", "528 Shutter-TriggerSequenceLength": "1000", "405 Shutter-Block voltage": "false", "XLIGHT Spin motor-State": "1", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "Vincent-D3-Time to open (ms)": "35.0000", "Vincent-D3-Time to close (ms)": "35.0000", "445 AO-TriggerSampleRate": "100000", "640 Shutter-IOChannel": "Dev1/ao14", "555 AO-Sequenceable": "1", "520 AO-Name": "AnalogIO", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "470 AO-Block voltage": "false", "COM1-Verbose": "1", "COM9-AnswerTimeout": "10000.0000", "528 Shutter-Name": "AnalogIO", "PRIME-Trigger Timeout (secs)": "10", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "NextFrame": 0, "470 AO-TriggerSequenceLength": "1000", "PRIME-TransposeXY": "0", "445 AO-TriggerSequenceLength": "1000", "COM1-Name": "COM1", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "COM9-DataBits": "8", "COM1-AnswerTimeout": "500.0000", "Camera": "", "TE2000-Version": "V2.50-V2.50-V2.10", "528 Shutter-Volts": "0.0000", "XLIGHT Hub-Name": "XLIGHT Hub", "PIEZO-Description": "Ludl stage driver adapter", "PositionName": "Pos17", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSampleRate": "100000", "PRIME-FanSpeedSetpoint": "High", "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "405 AO-TriggerSampleRate": "100000", "470 Shutter-Block voltage": "false", "470-State": "0", "COM3-Parity": "None", "405 AO-Volts": "0.0000", "PRIME-TransposeMirrorY": "0", "PRIME-ChipName": "GS144BSI", "470 AO-Volts": "0.0000", "PRIME-TransposeMirrorX": "0", "445 Shutter-SupportsTriggering": "0", "470 Shutter-SupportsTriggering": "0", "XLIGHT Hub-Description": "XLIGHT hub", "PRIME-PreampOffLimit": "10000", "470 AO-Sequenceable": "1", "528 Shutter-MaxVolts": "5.0000", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "Core-XYStage": "XYStage", "470 AO-TriggerSampleRate": "100000", "PRIME-Timing-PreTriggerDelayNs": "24672000.0000", "528-DA Device": "528 Shutter", "Focus-Description": "Nikon TE2000 focus adapter", "FilterBlock-State": "5", "520 AO-Demo": "No", "COM3-DelayBetweenCharsMs": "0.0000", "470 Shutter-Volts": "0.0000", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "PRIME-PP 2 THRESHOLD": "125", "PRIME-PP 4 THRESHOLD": "75", "XYStage-Acceleration": "4.0000", "470 Shutter-Demo": "No", "PRIME-Name": "PMPCIECam00", "640-DA Device": "640 Shutter", "405 AO-Block voltage": "false", "ElapsedTime-ms": 734213, "PRIME-Offset": "100", "445 AO-IOChannel": "Dev1/ao1", "640 AO-MaxVolts": "5.0000", "PositionIndex": 17, "PRIME-PP 1 ENABLED": "Yes", "COM1-BaudRate": "9600", "640-State": "1", "COM3-Verbose": "1", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "LudlController-TransmissionDelay": "0", "PRIME-FullWellCapacity": "62000", "PRIME-SMARTStreamingEnabled": "No", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "640 Shutter-Description": "NI DAC", "528-Description": "DA device that is used as a shutter", "COM4-Description": "Serial port driver (boost:asio)", "405 Shutter-Description": "NI DAC", "TE2000-Name": "TE2000", "528 Shutter-Description": "NI DAC", "COM9-Description": "Serial port driver (boost:asio)", "LudlController-Name": "LudlController", "445 Shutter-TriggerSequenceLength": "1000", "PRIME-SerialNumber": "A18B203008", "445 AO-Volts": "0.0000", "PRIME-X-dimension": "1200", "PRIME-ShutterCloseDelay": "0", "XLIGHT Disk slider-State": "2", "Position": "Default", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 AO-IOChannel": "Dev1/ao5", "520 AO-IOChannel": "Dev1/ao4", "528 AO-TriggerSequenceLength": "1000", "405 Shutter-MaxVolts": "5.0000", "470-DA Device": "470 Shutter", "PRIME-FTCapable": "No", "Core-ChannelGroup": "Channels", "555 AO-SupportsTriggering": "0", "PRIME-Y-dimension": "1200", "Frame": 0, "555 Shutter-Sequenceable": "1", "PRIME-Timing-ExposureTimeNs": "499998000.0000", "Core-ImageProcessor": "", "528-State": "0", "520 AO-Block voltage": "false", "XLIGHT Excitation Wheel-Label": "QUAD", "640 Shutter-Demo": "No", "COM3-StopBits": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "520 Shutter-IOChannel": "Dev1/ao11", "Width": 1200, "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-SupportsTriggering": "0", "640 AO-TriggerSampleRate": "100000", "520-Description": "DA device that is used as a shutter", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "405 Shutter-Sequenceable": "1", "405 Shutter-SupportsTriggering": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-TriggerSampleRate": "100000", "640 Shutter-MaxVolts": "5.0000", "555 AO-MaxVolts": "5.0000", "520 AO-TriggerSequenceLength": "1000", "COM3-BaudRate": "9600", "COM9-BaudRate": "9600", "Vincent-D3-Channel #": "Ch. #1", "PRIME-PMode": "Normal", "OpticalPath-Label": "Left(100)", "BitDepth": 16, "555 AO-TriggerSequenceLength": "1000", "445 Shutter-Description": "NI DAC", "405 AO-Sequenceable": "1", "PRIME-AcquisitionMethod": "Callbacks", "555 AO-Name": "AnalogIO", "PRIME-ShutterMode": "Pre-Exposure", "COM3-Handshaking": "Off", "445 AO-SupportsTriggering": "0", "ROI": "0-0-1200-1200", "COM9-Verbose": "1", "PRIME-Color": "OFF", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "PRIME-ShutterOpenDelay": "0", "COM1-Parity": "None", "Slice": 10, "SlicePosition": 50, "COM9-DelayBetweenCharsMs": "0.0000", "555-Name": "DA Shutter", "PIEZO-LudlSingleAxisName": "B", "XLIGHT Emission Wheel-Label": "679/29 (A)", "640 Shutter-Name": "AnalogIO", "640 AO-Sequenceable": "1", "520 Shutter-Demo": "No", "528 Shutter-Demo": "No", "470-Name": "DA Shutter", "520-DA Device": "520 Shutter", "XYStage-Description": "Ludl XY stage driver adapter", "445 Shutter-MinVolts": "0.0000", "528 AO-TriggerSampleRate": "100000", "405 AO-MinVolts": "0.0000", "405 Shutter-TriggerSequenceLength": "1000", "Height": 1200, "555 AO-Block voltage": "false", "640 Shutter-Volts": "5.0000", "PixelType": "GRAY16", "445 Shutter-Block voltage": "false", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "520 Shutter-TriggerSequenceLength": "1000", "COM9-Name": "COM9", "Core-SLM": "", "555 AO-Volts": "0.0000", "555 Shutter-Block voltage": "false", "XYStage-Speed": "1800.0000", "520 AO-MaxVolts": "5.0000", "528 Shutter-SupportsTriggering": "0", "COM1-Description": "Serial port driver (boost:asio)", "PRIME-MetadataEnabled": "No", "520 Shutter-MinVolts": "0.0000", "470 Shutter-Sequenceable": "1", "PRIME-PreampDelay": "0", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-MinVolts": "0.0000", "ChannelIndex": 2, "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Core-AutoShutter": "1", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "Exposure-ms": 320, "PRIME-PP 2 ENABLED": "Yes", "640 Shutter-TriggerSampleRate": "100000", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "470 Shutter-IOChannel": "Dev1/ao10", "405 AO-Demo": "No", "PixelSizeUm": 1.1, "470 AO-Demo": "No", "470 Shutter-MaxVolts": "5.0000", "Vincent-D3-Name": "Vincent-D3", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FileName": "img_000000000_Il-A_010.tif", "LudlController-Reset": "Operate", "PRIME-Exposure": "320.00", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "445-Name": "DA Shutter", "555 Shutter-Name": "AnalogIO", "520 Shutter-TriggerSampleRate": "100000", "445 Shutter-IOChannel": "Dev1/ao9", "PRIME-TransposeCorrection": "0", "XYStage-StepSize": "0.1000", "445-Description": "DA device that is used as a shutter", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 AO-IOChannel": "Dev1/ao7", "520 Shutter-Name": "AnalogIO", "COM3-DataBits": "8", "640 AO-Volts": "4.5000", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "555 Shutter-IOChannel": "Dev1/ao13", "WaitInterval": null, "520 Shutter-Sequenceable": "1", "YPositionUm": -13012.300000000001, "445-State": "0", "445-DA Device": "445 Shutter", "PRIME-CircularBufferAutoSize": "ON", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "555 AO-TriggerSampleRate": "100000", "445 Shutter-Volts": "0.0000", "XLIGHT Hub-Port": "COM3", "Core-AutoFocus": "", "COM4-Handshaking": "Off", "528 Shutter-MinVolts": "0.0000", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "445 Shutter-Name": "AnalogIO", "528 Shutter-Sequenceable": "1", "XYStage-TransposeMirrorX": "0", "XYStage-TransposeMirrorY": "0", "PRIME-ReadoutRate": "100MHz 16bit", "XYStage-Name": "Stage", "640 AO-SupportsTriggering": "0", "640-Name": "DA Shutter", "640 Shutter-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "640 AO-Block voltage": "false", "445 Shutter-Sequenceable": "1", "470 AO-MaxVolts": "5.0000", "COM4-BaudRate": "9600", "470-Description": "DA device that is used as a shutter", "520 Shutter-Description": "NI DAC", "PRIME-CCDTemperature": "-14.9200", "Time": "2019-12-17 03:59:51 -0500", "640 AO-TriggerSequenceLength": "1000", "470 AO-Name": "AnalogIO", "XLIGHT Disk slider-Label": "Disk Pos 40um", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "Binning": "1x1", "TE2000-ModelType": "1", "COM3-AnswerTimeout": "500.0000", "555 Shutter-SupportsTriggering": "0", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "TE2000-Port": "COM4", "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Description": "NI DAC", "COM4-AnswerTimeout": "500.0000", "COM1-DelayBetweenCharsMs": "0.0000", "FilterBlock-Label": "Confocal", "445 AO-Name": "AnalogIO", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-Timing-ReadoutTimeNs": "24672000.0000", "528 AO-Demo": "No", "520 AO-Sequenceable": "1", "555-Description": "DA device that is used as a shutter", "405-DA Device": "405 Shutter", "405 AO-TriggerSequenceLength": "1000", "640 AO-MinVolts": "0.0000", "640 Shutter-Block voltage": "false", "FrameIndex": 0, "PRIME-Port": "CMOS", "555 AO-MinVolts": "0.0000", "COM3-Description": "Serial port driver (boost:asio)", "528 AO-Description": "NI DAC", "555 AO-IOChannel": "Dev1/ao6", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "555 AO-TriggerInputLine": "/Dev1/PFI0", "PRIME-ActualInterval-ms": "84.6200", "LudlController-Port": "COM9", "445 Shutter-Demo": "No", "PRIME-Trigger-Expose Out-Mux": "1", "SliceIndex": 10, "PRIME-CCDTemperatureSetPoint": "-15.0000", "405 Shutter-MinVolts": "0.0000", "PRIME-ExposeOutMode": "First Row", "AxisPositions": null, "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Volts": "0.0000", "528-Name": "DA Shutter", "555-State": "0", "XLIGHT Dichroic-Label": "Di03-R635-t1", "445 AO-Sequenceable": "1", "405-Name": "DA Shutter", "405 AO-IOChannel": "Dev1/ao0", "Core-Camera": "PRIME", "Focus-Name": "Focus", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "405 Shutter-IOChannel": "Dev1/ao8", "640 AO-Demo": "No", "640 Shutter-SupportsTriggering": "0", "445 AO-Demo": "No", "555-DA Device": "555 Shutter", "470 Shutter-Description": "NI DAC", "528 AO-Volts": "0.0000", "COM1-DataBits": "8", "Vincent-D3-Address": "x", "520 AO-SupportsTriggering": "0", "405-Description": "DA device that is used as a shutter", "640 Shutter-MinVolts": "0.0000", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "405 AO-Name": "AnalogIO", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "640 AO-TriggerInputLine": "/Dev1/PFI0", "405 AO-TriggerInputLine": "/Dev1/PFI0", "640-Description": "DA device that is used as a shutter", "445 Shutter-TriggerSampleRate": "100000", "470 Shutter-TriggerSequenceLength": "1000", "COM1-StopBits": "1", "520 Shutter-SupportsTriggering": "0", "405-State": "0", "Core-Initialize": "1", "XLIGHT Emission Wheel-State": "3", "520 AO-Description": "NI DAC", "528 AO-TriggerInputLine": "/Dev1/PFI0", "445 Shutter-MaxVolts": "5.0000", "COM9-Handshaking": "Off", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "XLIGHT Spin motor-Label": "On (spinning)", "PRIME-PP 5 ENABLED": "No", "UUID": "4acccd63-5395-4380-a74a-761570b8f26e", "PRIME-TriggerMode": "Internal Trigger", "COM1-Handshaking": "Off", "PRIME-Timing-PostTriggerDelayNs": "49344000.0000", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "Focus-Position": "16732.9000", "528 Shutter-Block voltage": "false", "Channel": "Il-A", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "555 Shutter-Demo": "No", "405 Shutter-Demo": "No", "528 AO-Name": "AnalogIO", "520-State": "0", "Core-Galvo": "", "405 Shutter-TriggerSampleRate": "100000", "Focus-FocusStepSize_(nm)": "100", "PRIME-PixelType": "16bit", "COM4-DataBits": "8", "470 AO-IOChannel": "Dev1/ao2", "520 AO-MinVolts": "0.0000", "COM9-Parity": "None", "PRIME-CircularBufferEnabled": "ON", "PRIME-PP 6 Reset": "No", "640 AO-Description": "NI DAC", "Source": "PRIME", "445 AO-MinVolts": "0.0000", "PIEZO-Name": "Stage", "445 AO-Description": "NI DAC", "PRIME-CircularBufferFrameCount": "46", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "520 AO-Volts": "0.0000", "528 Shutter-TriggerSampleRate": "100000", "XPositionUm": 23269.100000000002, "OpticalPath-State": "4", "PRIME-PP 3 THRESHOLD": "80", "PRIME-PVCAM Version": "3.7.1", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Gain": "1-HDR", "640 Shutter-TriggerSequenceLength": "1000", "528 AO-SupportsTriggering": "0", "445 AO-TriggerInputLine": "/Dev1/PFI0", "OpticalPath-Name": "OpticalPath", "PRIME-PP 4 ENABLED": "Yes", "PRIME-PVCAM Adapter Version": "1.3.29", "COM3-Name": "COM3", "405 AO-MaxVolts": "5.0000", "520 Shutter-Block voltage": "false", "PRIME-Timing-ClearingTimeNs": "49344000.0000", "Core-Focus": "PIEZO", "528 AO-Block voltage": "false", "TE2000-Description": "Nikon TE2000 hub", "528 AO-MinVolts": "0.0000", "COM4-Parity": "None", "Summary": { "Slices": 11, "ChContrastMin": [ 0, 0, 0, 0, 0, 0 ], "PixelAspect": 1, "SlicesFirst": true, "Interval_ms": 0, "Time": "2019-12-17 03:59:33 -0500", "Prefix": "seq_7", "InitialPositionList": [ { "Label": "Pos0", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [149.91], "Focus": [16657.7], "XYStage": [ 71629.7, 19152.9 ] }, "GridColumnIndex": 0 }, { "Label": "Pos1", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [190.82], "Focus": [16485.4], "XYStage": [ 65702.3, 19396.600000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos2", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [194.69], "Focus": [16446.8], "XYStage": [ 59880.5, 19007.8 ] }, "GridColumnIndex": 0 }, { "Label": "Pos3", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [145.04], "Focus": [16446.6], "XYStage": [ 53616.9, 18818.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos4", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [169.57500000000002], "Focus": [16446.5], "XYStage": [ 47662.8, 18817.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos5", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [131.805], "Focus": [16446.4], "XYStage": [ 41918.8, 18298.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos6", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [113.145], "Focus": [16446.5], "XYStage": [ 36129.9, 18895.8 ] }, "GridColumnIndex": 0 }, { "Label": "Pos7", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [141.005], "Focus": [16446.6], "XYStage": [ 30299.100000000002, 18895.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos8", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [140.97], "Focus": [16446.5], "XYStage": [ 23952.100000000002, 19156 ] }, "GridColumnIndex": 0 }, { "Label": "Pos9", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [184.57500000000002], "Focus": [16677.9], "XYStage": [ 72866.8, -14809.300000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos10", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [167.95000000000002], "Focus": [16557.9], "XYStage": [ 66515.6, -14371.300000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos11", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [168], "Focus": [16618.9], "XYStage": [ 59724.3, -13964.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos12", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [203.205], "Focus": [16779.6], "XYStage": [ 53862.3, -13494.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos13", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [203.20000000000002], "Focus": [16749], "XYStage": [ 48108.3, -13805.400000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos14", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [203.19], "Focus": [16675.9], "XYStage": [ 41645, -13804.900000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos15", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [203.21], "Focus": [16675.7], "XYStage": [ 35352.6, -13805.6 ] }, "GridColumnIndex": 0 }, { "Label": "Pos16", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [203.225], "Focus": [16675.8], "XYStage": [ 29298, -13447.400000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos17", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [203.215], "Focus": [16675.8], "XYStage": [ 23269.100000000002, -13012.300000000001 ] }, "GridColumnIndex": 0 } ], "z-step_um": 10, "GridRow": 0, "BitDepth": 16, "Depth": 2, "Channels": 6, "Source": "Micro-Manager", "ChColors": [ -16711681, -256, -65281, -1, -1, -16776961 ], "MetadataVersion": 10, "KeepShutterOpenChannels": true, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536, 65536 ], "CustomIntervals_ms": [], "Height": 1200, "MicroManagerVersion": "1.4.23 20180219", "GridColumn": 0, "UUID": "3ad8801b-5699-40d6-813b-4ad840863e38", "PixelType": "GRAY16", "Width": 1200, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\Yan\\20191216-YC113-S11S12-miseq", "Frames": 1, "Comment": "", "UserName": "Nikon", "PixelSize_um": 1.1, "ROI": [ 0, 0, 1200, 1200 ], "Date": "2019-12-17", "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC", "P-DAPI-high" ], "KeepShutterOpenSlices": true, "IJType": 1, "ComputerName": "ZadorConfocal2", "Positions": 18, "PositionIndex": 17, "CameraTimeout": 20000, "TimeFirst": false }, "640 AO-Name": "AnalogIO", "Vincent-D3-Command": "Close" }, "FrameKey-0-3-0": { "COM4-StopBits": "1", "528 AO-MaxVolts": "5.0000", "XLIGHT Excitation Wheel-State": "1", "470 Shutter-Name": "AnalogIO", "Core-Shutter": "640", "555 AO-Demo": "No", "XLIGHT Dichroic-State": "2", "XYStage-StepSize-Y": "0.1000", "ZPositionUm": 153.215, "XYStage-StepSize-X": "0.1000", "PRIME-FirmwareVersion": "20.28", "PRIME-ClearCycles": "2", "520-Name": "DA Shutter", "405 Shutter-Name": "AnalogIO", "PIEZO-StepSize": "0.0050", "PRIME-ClearMode": "Pre-Exposure", "470 AO-MinVolts": "0.0000", "405 AO-SupportsTriggering": "1", "CameraChannelIndex": 0, "XYStage-StartSpeed": "500.0000", "COM9-StopBits": "2", "405 AO-Description": "NI DAC", "COM4-DelayBetweenCharsMs": "0.0000", "445 AO-Block voltage": "false", "520 AO-TriggerInputLine": "/Dev1/PFI0", "555 AO-Description": "NI DAC", "520 AO-TriggerSampleRate": "100000", "COM4-Verbose": "1", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM4-Name": "COM4", "405 Shutter-Volts": "0.0000", "Vincent-D3-Port": "COM1", "PRIME-PP 4 MIN ADU AFFECTED": "200", "470 Shutter-MinVolts": "0.0000", "PRIME-PP 3 ENABLED": "Yes", "470 AO-Description": "NI DAC", "PRIME-Description": "PVCAM API device adapter", "520 Shutter-MaxVolts": "5.0000", "520 Shutter-Volts": "0.0000", "528 AO-Sequenceable": "1", "PRIME-Binning": "1x1", "555 Shutter-MaxVolts": "5.0000", "528 Shutter-TriggerSequenceLength": "1000", "405 Shutter-Block voltage": "false", "XLIGHT Spin motor-State": "1", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "Vincent-D3-Time to open (ms)": "35.0000", "Vincent-D3-Time to close (ms)": "35.0000", "445 AO-TriggerSampleRate": "100000", "640 Shutter-IOChannel": "Dev1/ao14", "555 AO-Sequenceable": "1", "520 AO-Name": "AnalogIO", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "470 AO-Block voltage": "false", "COM1-Verbose": "1", "COM9-AnswerTimeout": "10000.0000", "528 Shutter-Name": "AnalogIO", "PRIME-Trigger Timeout (secs)": "10", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "NextFrame": 0, "470 AO-TriggerSequenceLength": "1000", "PRIME-TransposeXY": "0", "445 AO-TriggerSequenceLength": "1000", "COM1-Name": "COM1", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "COM9-DataBits": "8", "COM1-AnswerTimeout": "500.0000", "Camera": "", "TE2000-Version": "V2.50-V2.50-V2.10", "528 Shutter-Volts": "0.0000", "XLIGHT Hub-Name": "XLIGHT Hub", "PIEZO-Description": "Ludl stage driver adapter", "PositionName": "Pos17", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSampleRate": "100000", "PRIME-FanSpeedSetpoint": "High", "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "405 AO-TriggerSampleRate": "100000", "470 Shutter-Block voltage": "false", "470-State": "0", "COM3-Parity": "None", "405 AO-Volts": "0.0000", "PRIME-TransposeMirrorY": "0", "PRIME-ChipName": "GS144BSI", "470 AO-Volts": "0.0000", "PRIME-TransposeMirrorX": "0", "445 Shutter-SupportsTriggering": "0", "470 Shutter-SupportsTriggering": "0", "XLIGHT Hub-Description": "XLIGHT hub", "PRIME-PreampOffLimit": "10000", "470 AO-Sequenceable": "1", "528 Shutter-MaxVolts": "5.0000", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "Core-XYStage": "XYStage", "470 AO-TriggerSampleRate": "100000", "PRIME-Timing-PreTriggerDelayNs": "24672000.0000", "528-DA Device": "528 Shutter", "Focus-Description": "Nikon TE2000 focus adapter", "FilterBlock-State": "5", "520 AO-Demo": "No", "COM3-DelayBetweenCharsMs": "0.0000", "470 Shutter-Volts": "0.0000", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "PRIME-PP 2 THRESHOLD": "125", "PRIME-PP 4 THRESHOLD": "75", "XYStage-Acceleration": "4.0000", "470 Shutter-Demo": "No", "PRIME-Name": "PMPCIECam00", "640-DA Device": "640 Shutter", "405 AO-Block voltage": "false", "ElapsedTime-ms": 735325, "PRIME-Offset": "100", "445 AO-IOChannel": "Dev1/ao1", "640 AO-MaxVolts": "5.0000", "PositionIndex": 17, "PRIME-PP 1 ENABLED": "Yes", "COM1-BaudRate": "9600", "640-State": "1", "COM3-Verbose": "1", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "LudlController-TransmissionDelay": "0", "PRIME-FullWellCapacity": "62000", "PRIME-SMARTStreamingEnabled": "No", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "640 Shutter-Description": "NI DAC", "528-Description": "DA device that is used as a shutter", "COM4-Description": "Serial port driver (boost:asio)", "405 Shutter-Description": "NI DAC", "TE2000-Name": "TE2000", "528 Shutter-Description": "NI DAC", "COM9-Description": "Serial port driver (boost:asio)", "LudlController-Name": "LudlController", "445 Shutter-TriggerSequenceLength": "1000", "PRIME-SerialNumber": "A18B203008", "445 AO-Volts": "0.0000", "PRIME-X-dimension": "1200", "PRIME-ShutterCloseDelay": "0", "XLIGHT Disk slider-State": "2", "Position": "Default", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 AO-IOChannel": "Dev1/ao5", "520 AO-IOChannel": "Dev1/ao4", "528 AO-TriggerSequenceLength": "1000", "405 Shutter-MaxVolts": "5.0000", "470-DA Device": "470 Shutter", "PRIME-FTCapable": "No", "Core-ChannelGroup": "Channels", "555 AO-SupportsTriggering": "0", "PRIME-Y-dimension": "1200", "Frame": 0, "555 Shutter-Sequenceable": "1", "PRIME-Timing-ExposureTimeNs": "499998000.0000", "Core-ImageProcessor": "", "528-State": "0", "520 AO-Block voltage": "false", "XLIGHT Excitation Wheel-Label": "QUAD", "640 Shutter-Demo": "No", "COM3-StopBits": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "520 Shutter-IOChannel": "Dev1/ao11", "Width": 1200, "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-SupportsTriggering": "0", "640 AO-TriggerSampleRate": "100000", "520-Description": "DA device that is used as a shutter", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "405 Shutter-Sequenceable": "1", "405 Shutter-SupportsTriggering": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-TriggerSampleRate": "100000", "640 Shutter-MaxVolts": "5.0000", "555 AO-MaxVolts": "5.0000", "520 AO-TriggerSequenceLength": "1000", "COM3-BaudRate": "9600", "COM9-BaudRate": "9600", "Vincent-D3-Channel #": "Ch. #1", "PRIME-PMode": "Normal", "OpticalPath-Label": "Left(100)", "BitDepth": 16, "555 AO-TriggerSequenceLength": "1000", "445 Shutter-Description": "NI DAC", "405 AO-Sequenceable": "1", "PRIME-AcquisitionMethod": "Callbacks", "555 AO-Name": "AnalogIO", "PRIME-ShutterMode": "Pre-Exposure", "COM3-Handshaking": "Off", "445 AO-SupportsTriggering": "0", "ROI": "0-0-1200-1200", "COM9-Verbose": "1", "PRIME-Color": "OFF", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "PRIME-ShutterOpenDelay": "0", "COM1-Parity": "None", "Slice": 0, "SlicePosition": -50, "COM9-DelayBetweenCharsMs": "0.0000", "555-Name": "DA Shutter", "PIEZO-LudlSingleAxisName": "B", "XLIGHT Emission Wheel-Label": "725/40 (C)", "640 Shutter-Name": "AnalogIO", "640 AO-Sequenceable": "1", "520 Shutter-Demo": "No", "528 Shutter-Demo": "No", "470-Name": "DA Shutter", "520-DA Device": "520 Shutter", "XYStage-Description": "Ludl XY stage driver adapter", "445 Shutter-MinVolts": "0.0000", "528 AO-TriggerSampleRate": "100000", "405 AO-MinVolts": "0.0000", "405 Shutter-TriggerSequenceLength": "1000", "Height": 1200, "555 AO-Block voltage": "false", "640 Shutter-Volts": "5.0000", "PixelType": "GRAY16", "445 Shutter-Block voltage": "false", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "520 Shutter-TriggerSequenceLength": "1000", "COM9-Name": "COM9", "Core-SLM": "", "555 AO-Volts": "0.0000", "555 Shutter-Block voltage": "false", "XYStage-Speed": "1800.0000", "520 AO-MaxVolts": "5.0000", "528 Shutter-SupportsTriggering": "0", "COM1-Description": "Serial port driver (boost:asio)", "PRIME-MetadataEnabled": "No", "520 Shutter-MinVolts": "0.0000", "470 Shutter-Sequenceable": "1", "PRIME-PreampDelay": "0", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-MinVolts": "0.0000", "ChannelIndex": 3, "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Core-AutoShutter": "1", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "Exposure-ms": 520, "PRIME-PP 2 ENABLED": "Yes", "640 Shutter-TriggerSampleRate": "100000", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "470 Shutter-IOChannel": "Dev1/ao10", "405 AO-Demo": "No", "PixelSizeUm": 1.1, "470 AO-Demo": "No", "470 Shutter-MaxVolts": "5.0000", "Vincent-D3-Name": "Vincent-D3", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FileName": "img_000000000_Il-C_000.tif", "LudlController-Reset": "Operate", "PRIME-Exposure": "520.00", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "445-Name": "DA Shutter", "555 Shutter-Name": "AnalogIO", "520 Shutter-TriggerSampleRate": "100000", "445 Shutter-IOChannel": "Dev1/ao9", "PRIME-TransposeCorrection": "0", "XYStage-StepSize": "0.1000", "445-Description": "DA device that is used as a shutter", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 AO-IOChannel": "Dev1/ao7", "520 Shutter-Name": "AnalogIO", "COM3-DataBits": "8", "640 AO-Volts": "4.5000", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "555 Shutter-IOChannel": "Dev1/ao13", "WaitInterval": null, "520 Shutter-Sequenceable": "1", "YPositionUm": -13012.300000000001, "445-State": "0", "445-DA Device": "445 Shutter", "PRIME-CircularBufferAutoSize": "ON", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "555 AO-TriggerSampleRate": "100000", "445 Shutter-Volts": "0.0000", "XLIGHT Hub-Port": "COM3", "Core-AutoFocus": "", "COM4-Handshaking": "Off", "528 Shutter-MinVolts": "0.0000", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "445 Shutter-Name": "AnalogIO", "528 Shutter-Sequenceable": "1", "XYStage-TransposeMirrorX": "0", "XYStage-TransposeMirrorY": "0", "PRIME-ReadoutRate": "100MHz 16bit", "XYStage-Name": "Stage", "640 AO-SupportsTriggering": "0", "640-Name": "DA Shutter", "640 Shutter-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "640 AO-Block voltage": "false", "445 Shutter-Sequenceable": "1", "470 AO-MaxVolts": "5.0000", "COM4-BaudRate": "9600", "470-Description": "DA device that is used as a shutter", "520 Shutter-Description": "NI DAC", "PRIME-CCDTemperature": "-14.9200", "Time": "2019-12-17 03:59:52 -0500", "640 AO-TriggerSequenceLength": "1000", "470 AO-Name": "AnalogIO", "XLIGHT Disk slider-Label": "Disk Pos 40um", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "Binning": "1x1", "TE2000-ModelType": "1", "COM3-AnswerTimeout": "500.0000", "555 Shutter-SupportsTriggering": "0", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "TE2000-Port": "COM4", "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Description": "NI DAC", "COM4-AnswerTimeout": "500.0000", "COM1-DelayBetweenCharsMs": "0.0000", "FilterBlock-Label": "Confocal", "445 AO-Name": "AnalogIO", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-Timing-ReadoutTimeNs": "24672000.0000", "528 AO-Demo": "No", "520 AO-Sequenceable": "1", "555-Description": "DA device that is used as a shutter", "405-DA Device": "405 Shutter", "405 AO-TriggerSequenceLength": "1000", "640 AO-MinVolts": "0.0000", "640 Shutter-Block voltage": "false", "FrameIndex": 0, "PRIME-Port": "CMOS", "555 AO-MinVolts": "0.0000", "COM3-Description": "Serial port driver (boost:asio)", "528 AO-Description": "NI DAC", "555 AO-IOChannel": "Dev1/ao6", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "555 AO-TriggerInputLine": "/Dev1/PFI0", "PRIME-ActualInterval-ms": "84.6200", "LudlController-Port": "COM9", "445 Shutter-Demo": "No", "PRIME-Trigger-Expose Out-Mux": "1", "SliceIndex": 0, "PRIME-CCDTemperatureSetPoint": "-15.0000", "405 Shutter-MinVolts": "0.0000", "PRIME-ExposeOutMode": "First Row", "AxisPositions": null, "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Volts": "0.0000", "528-Name": "DA Shutter", "555-State": "0", "XLIGHT Dichroic-Label": "Di03-R635-t1", "445 AO-Sequenceable": "1", "405-Name": "DA Shutter", "405 AO-IOChannel": "Dev1/ao0", "Core-Camera": "PRIME", "Focus-Name": "Focus", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "405 Shutter-IOChannel": "Dev1/ao8", "640 AO-Demo": "No", "640 Shutter-SupportsTriggering": "0", "445 AO-Demo": "No", "555-DA Device": "555 Shutter", "470 Shutter-Description": "NI DAC", "528 AO-Volts": "0.0000", "COM1-DataBits": "8", "Vincent-D3-Address": "x", "520 AO-SupportsTriggering": "0", "405-Description": "DA device that is used as a shutter", "640 Shutter-MinVolts": "0.0000", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "405 AO-Name": "AnalogIO", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "640 AO-TriggerInputLine": "/Dev1/PFI0", "405 AO-TriggerInputLine": "/Dev1/PFI0", "640-Description": "DA device that is used as a shutter", "445 Shutter-TriggerSampleRate": "100000", "470 Shutter-TriggerSequenceLength": "1000", "COM1-StopBits": "1", "520 Shutter-SupportsTriggering": "0", "405-State": "0", "Core-Initialize": "1", "XLIGHT Emission Wheel-State": "3", "520 AO-Description": "NI DAC", "528 AO-TriggerInputLine": "/Dev1/PFI0", "445 Shutter-MaxVolts": "5.0000", "COM9-Handshaking": "Off", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "XLIGHT Spin motor-Label": "On (spinning)", "PRIME-PP 5 ENABLED": "No", "UUID": "ae0d658b-6080-4d3e-b657-f9e8e113f2aa", "PRIME-TriggerMode": "Internal Trigger", "COM1-Handshaking": "Off", "PRIME-Timing-PostTriggerDelayNs": "49344000.0000", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "Focus-Position": "16732.9000", "528 Shutter-Block voltage": "false", "Channel": "Il-C", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "555 Shutter-Demo": "No", "405 Shutter-Demo": "No", "528 AO-Name": "AnalogIO", "520-State": "0", "Core-Galvo": "", "405 Shutter-TriggerSampleRate": "100000", "Focus-FocusStepSize_(nm)": "100", "PRIME-PixelType": "16bit", "COM4-DataBits": "8", "470 AO-IOChannel": "Dev1/ao2", "520 AO-MinVolts": "0.0000", "COM9-Parity": "None", "PRIME-CircularBufferEnabled": "ON", "PRIME-PP 6 Reset": "No", "640 AO-Description": "NI DAC", "Source": "PRIME", "445 AO-MinVolts": "0.0000", "PIEZO-Name": "Stage", "445 AO-Description": "NI DAC", "PRIME-CircularBufferFrameCount": "46", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "520 AO-Volts": "0.0000", "528 Shutter-TriggerSampleRate": "100000", "XPositionUm": 23269.100000000002, "OpticalPath-State": "4", "PRIME-PP 3 THRESHOLD": "80", "PRIME-PVCAM Version": "3.7.1", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Gain": "1-HDR", "640 Shutter-TriggerSequenceLength": "1000", "528 AO-SupportsTriggering": "0", "445 AO-TriggerInputLine": "/Dev1/PFI0", "OpticalPath-Name": "OpticalPath", "PRIME-PP 4 ENABLED": "Yes", "PRIME-PVCAM Adapter Version": "1.3.29", "COM3-Name": "COM3", "405 AO-MaxVolts": "5.0000", "520 Shutter-Block voltage": "false", "PRIME-Timing-ClearingTimeNs": "49344000.0000", "Core-Focus": "PIEZO", "528 AO-Block voltage": "false", "TE2000-Description": "Nikon TE2000 hub", "528 AO-MinVolts": "0.0000", "COM4-Parity": "None", "Summary": { "Slices": 11, "ChContrastMin": [ 0, 0, 0, 0, 0, 0 ], "PixelAspect": 1, "SlicesFirst": true, "Interval_ms": 0, "Time": "2019-12-17 03:59:33 -0500", "Prefix": "seq_7", "InitialPositionList": [ { "Label": "Pos0", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [149.91], "Focus": [16657.7], "XYStage": [ 71629.7, 19152.9 ] }, "GridColumnIndex": 0 }, { "Label": "Pos1", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [190.82], "Focus": [16485.4], "XYStage": [ 65702.3, 19396.600000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos2", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [194.69], "Focus": [16446.8], "XYStage": [ 59880.5, 19007.8 ] }, "GridColumnIndex": 0 }, { "Label": "Pos3", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [145.04], "Focus": [16446.6], "XYStage": [ 53616.9, 18818.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos4", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [169.57500000000002], "Focus": [16446.5], "XYStage": [ 47662.8, 18817.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos5", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [131.805], "Focus": [16446.4], "XYStage": [ 41918.8, 18298.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos6", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [113.145], "Focus": [16446.5], "XYStage": [ 36129.9, 18895.8 ] }, "GridColumnIndex": 0 }, { "Label": "Pos7", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [141.005], "Focus": [16446.6], "XYStage": [ 30299.100000000002, 18895.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos8", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [140.97], "Focus": [16446.5], "XYStage": [ 23952.100000000002, 19156 ] }, "GridColumnIndex": 0 }, { "Label": "Pos9", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [184.57500000000002], "Focus": [16677.9], "XYStage": [ 72866.8, -14809.300000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos10", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [167.95000000000002], "Focus": [16557.9], "XYStage": [ 66515.6, -14371.300000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos11", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [168], "Focus": [16618.9], "XYStage": [ 59724.3, -13964.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos12", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [203.205], "Focus": [16779.6], "XYStage": [ 53862.3, -13494.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos13", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [203.20000000000002], "Focus": [16749], "XYStage": [ 48108.3, -13805.400000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos14", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [203.19], "Focus": [16675.9], "XYStage": [ 41645, -13804.900000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos15", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [203.21], "Focus": [16675.7], "XYStage": [ 35352.6, -13805.6 ] }, "GridColumnIndex": 0 }, { "Label": "Pos16", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [203.225], "Focus": [16675.8], "XYStage": [ 29298, -13447.400000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos17", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [203.215], "Focus": [16675.8], "XYStage": [ 23269.100000000002, -13012.300000000001 ] }, "GridColumnIndex": 0 } ], "z-step_um": 10, "GridRow": 0, "BitDepth": 16, "Depth": 2, "Channels": 6, "Source": "Micro-Manager", "ChColors": [ -16711681, -256, -65281, -1, -1, -16776961 ], "MetadataVersion": 10, "KeepShutterOpenChannels": true, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536, 65536 ], "CustomIntervals_ms": [], "Height": 1200, "MicroManagerVersion": "1.4.23 20180219", "GridColumn": 0, "UUID": "3ad8801b-5699-40d6-813b-4ad840863e38", "PixelType": "GRAY16", "Width": 1200, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\Yan\\20191216-YC113-S11S12-miseq", "Frames": 1, "Comment": "", "UserName": "Nikon", "PixelSize_um": 1.1, "ROI": [ 0, 0, 1200, 1200 ], "Date": "2019-12-17", "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC", "P-DAPI-high" ], "KeepShutterOpenSlices": true, "IJType": 1, "ComputerName": "ZadorConfocal2", "Positions": 18, "PositionIndex": 17, "CameraTimeout": 20000, "TimeFirst": false }, "640 AO-Name": "AnalogIO", "Vincent-D3-Command": "Close" }, "FrameKey-0-3-1": { "COM4-StopBits": "1", "528 AO-MaxVolts": "5.0000", "XLIGHT Excitation Wheel-State": "1", "470 Shutter-Name": "AnalogIO", "Core-Shutter": "640", "555 AO-Demo": "No", "XLIGHT Dichroic-State": "2", "XYStage-StepSize-Y": "0.1000", "ZPositionUm": 163.215, "XYStage-StepSize-X": "0.1000", "PRIME-FirmwareVersion": "20.28", "PRIME-ClearCycles": "2", "520-Name": "DA Shutter", "405 Shutter-Name": "AnalogIO", "PIEZO-StepSize": "0.0050", "PRIME-ClearMode": "Pre-Exposure", "470 AO-MinVolts": "0.0000", "405 AO-SupportsTriggering": "1", "CameraChannelIndex": 0, "XYStage-StartSpeed": "500.0000", "COM9-StopBits": "2", "405 AO-Description": "NI DAC", "COM4-DelayBetweenCharsMs": "0.0000", "445 AO-Block voltage": "false", "520 AO-TriggerInputLine": "/Dev1/PFI0", "555 AO-Description": "NI DAC", "520 AO-TriggerSampleRate": "100000", "COM4-Verbose": "1", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM4-Name": "COM4", "405 Shutter-Volts": "0.0000", "Vincent-D3-Port": "COM1", "PRIME-PP 4 MIN ADU AFFECTED": "200", "470 Shutter-MinVolts": "0.0000", "PRIME-PP 3 ENABLED": "Yes", "470 AO-Description": "NI DAC", "PRIME-Description": "PVCAM API device adapter", "520 Shutter-MaxVolts": "5.0000", "520 Shutter-Volts": "0.0000", "528 AO-Sequenceable": "1", "PRIME-Binning": "1x1", "555 Shutter-MaxVolts": "5.0000", "528 Shutter-TriggerSequenceLength": "1000", "405 Shutter-Block voltage": "false", "XLIGHT Spin motor-State": "1", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "Vincent-D3-Time to open (ms)": "35.0000", "Vincent-D3-Time to close (ms)": "35.0000", "445 AO-TriggerSampleRate": "100000", "640 Shutter-IOChannel": "Dev1/ao14", "555 AO-Sequenceable": "1", "520 AO-Name": "AnalogIO", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "470 AO-Block voltage": "false", "COM1-Verbose": "1", "COM9-AnswerTimeout": "10000.0000", "528 Shutter-Name": "AnalogIO", "PRIME-Trigger Timeout (secs)": "10", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "NextFrame": 0, "470 AO-TriggerSequenceLength": "1000", "PRIME-TransposeXY": "0", "445 AO-TriggerSequenceLength": "1000", "COM1-Name": "COM1", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "COM9-DataBits": "8", "COM1-AnswerTimeout": "500.0000", "Camera": "", "TE2000-Version": "V2.50-V2.50-V2.10", "528 Shutter-Volts": "0.0000", "XLIGHT Hub-Name": "XLIGHT Hub", "PIEZO-Description": "Ludl stage driver adapter", "PositionName": "Pos17", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSampleRate": "100000", "PRIME-FanSpeedSetpoint": "High", "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "405 AO-TriggerSampleRate": "100000", "470 Shutter-Block voltage": "false", "470-State": "0", "COM3-Parity": "None", "405 AO-Volts": "0.0000", "PRIME-TransposeMirrorY": "0", "PRIME-ChipName": "GS144BSI", "470 AO-Volts": "0.0000", "PRIME-TransposeMirrorX": "0", "445 Shutter-SupportsTriggering": "0", "470 Shutter-SupportsTriggering": "0", "XLIGHT Hub-Description": "XLIGHT hub", "PRIME-PreampOffLimit": "10000", "470 AO-Sequenceable": "1", "528 Shutter-MaxVolts": "5.0000", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "Core-XYStage": "XYStage", "470 AO-TriggerSampleRate": "100000", "PRIME-Timing-PreTriggerDelayNs": "24672000.0000", "528-DA Device": "528 Shutter", "Focus-Description": "Nikon TE2000 focus adapter", "FilterBlock-State": "5", "520 AO-Demo": "No", "COM3-DelayBetweenCharsMs": "0.0000", "470 Shutter-Volts": "0.0000", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "PRIME-PP 2 THRESHOLD": "125", "PRIME-PP 4 THRESHOLD": "75", "XYStage-Acceleration": "4.0000", "470 Shutter-Demo": "No", "PRIME-Name": "PMPCIECam00", "640-DA Device": "640 Shutter", "405 AO-Block voltage": "false", "ElapsedTime-ms": 736092, "PRIME-Offset": "100", "445 AO-IOChannel": "Dev1/ao1", "640 AO-MaxVolts": "5.0000", "PositionIndex": 17, "PRIME-PP 1 ENABLED": "Yes", "COM1-BaudRate": "9600", "640-State": "1", "COM3-Verbose": "1", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "LudlController-TransmissionDelay": "0", "PRIME-FullWellCapacity": "62000", "PRIME-SMARTStreamingEnabled": "No", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "640 Shutter-Description": "NI DAC", "528-Description": "DA device that is used as a shutter", "COM4-Description": "Serial port driver (boost:asio)", "405 Shutter-Description": "NI DAC", "TE2000-Name": "TE2000", "528 Shutter-Description": "NI DAC", "COM9-Description": "Serial port driver (boost:asio)", "LudlController-Name": "LudlController", "445 Shutter-TriggerSequenceLength": "1000", "PRIME-SerialNumber": "A18B203008", "445 AO-Volts": "0.0000", "PRIME-X-dimension": "1200", "PRIME-ShutterCloseDelay": "0", "XLIGHT Disk slider-State": "2", "Position": "Default", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 AO-IOChannel": "Dev1/ao5", "520 AO-IOChannel": "Dev1/ao4", "528 AO-TriggerSequenceLength": "1000", "405 Shutter-MaxVolts": "5.0000", "470-DA Device": "470 Shutter", "PRIME-FTCapable": "No", "Core-ChannelGroup": "Channels", "555 AO-SupportsTriggering": "0", "PRIME-Y-dimension": "1200", "Frame": 0, "555 Shutter-Sequenceable": "1", "PRIME-Timing-ExposureTimeNs": "499998000.0000", "Core-ImageProcessor": "", "528-State": "0", "520 AO-Block voltage": "false", "XLIGHT Excitation Wheel-Label": "QUAD", "640 Shutter-Demo": "No", "COM3-StopBits": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "520 Shutter-IOChannel": "Dev1/ao11", "Width": 1200, "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-SupportsTriggering": "0", "640 AO-TriggerSampleRate": "100000", "520-Description": "DA device that is used as a shutter", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "405 Shutter-Sequenceable": "1", "405 Shutter-SupportsTriggering": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-TriggerSampleRate": "100000", "640 Shutter-MaxVolts": "5.0000", "555 AO-MaxVolts": "5.0000", "520 AO-TriggerSequenceLength": "1000", "COM3-BaudRate": "9600", "COM9-BaudRate": "9600", "Vincent-D3-Channel #": "Ch. #1", "PRIME-PMode": "Normal", "OpticalPath-Label": "Left(100)", "BitDepth": 16, "555 AO-TriggerSequenceLength": "1000", "445 Shutter-Description": "NI DAC", "405 AO-Sequenceable": "1", "PRIME-AcquisitionMethod": "Callbacks", "555 AO-Name": "AnalogIO", "PRIME-ShutterMode": "Pre-Exposure", "COM3-Handshaking": "Off", "445 AO-SupportsTriggering": "0", "ROI": "0-0-1200-1200", "COM9-Verbose": "1", "PRIME-Color": "OFF", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "PRIME-ShutterOpenDelay": "0", "COM1-Parity": "None", "Slice": 1, "SlicePosition": -40, "COM9-DelayBetweenCharsMs": "0.0000", "555-Name": "DA Shutter", "PIEZO-LudlSingleAxisName": "B", "XLIGHT Emission Wheel-Label": "725/40 (C)", "640 Shutter-Name": "AnalogIO", "640 AO-Sequenceable": "1", "520 Shutter-Demo": "No", "528 Shutter-Demo": "No", "470-Name": "DA Shutter", "520-DA Device": "520 Shutter", "XYStage-Description": "Ludl XY stage driver adapter", "445 Shutter-MinVolts": "0.0000", "528 AO-TriggerSampleRate": "100000", "405 AO-MinVolts": "0.0000", "405 Shutter-TriggerSequenceLength": "1000", "Height": 1200, "555 AO-Block voltage": "false", "640 Shutter-Volts": "5.0000", "PixelType": "GRAY16", "445 Shutter-Block voltage": "false", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "520 Shutter-TriggerSequenceLength": "1000", "COM9-Name": "COM9", "Core-SLM": "", "555 AO-Volts": "0.0000", "555 Shutter-Block voltage": "false", "XYStage-Speed": "1800.0000", "520 AO-MaxVolts": "5.0000", "528 Shutter-SupportsTriggering": "0", "COM1-Description": "Serial port driver (boost:asio)", "PRIME-MetadataEnabled": "No", "520 Shutter-MinVolts": "0.0000", "470 Shutter-Sequenceable": "1", "PRIME-PreampDelay": "0", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-MinVolts": "0.0000", "ChannelIndex": 3, "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Core-AutoShutter": "1", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "Exposure-ms": 520, "PRIME-PP 2 ENABLED": "Yes", "640 Shutter-TriggerSampleRate": "100000", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "470 Shutter-IOChannel": "Dev1/ao10", "405 AO-Demo": "No", "PixelSizeUm": 1.1, "470 AO-Demo": "No", "470 Shutter-MaxVolts": "5.0000", "Vincent-D3-Name": "Vincent-D3", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FileName": "img_000000000_Il-C_001.tif", "LudlController-Reset": "Operate", "PRIME-Exposure": "520.00", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "445-Name": "DA Shutter", "555 Shutter-Name": "AnalogIO", "520 Shutter-TriggerSampleRate": "100000", "445 Shutter-IOChannel": "Dev1/ao9", "PRIME-TransposeCorrection": "0", "XYStage-StepSize": "0.1000", "445-Description": "DA device that is used as a shutter", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 AO-IOChannel": "Dev1/ao7", "520 Shutter-Name": "AnalogIO", "COM3-DataBits": "8", "640 AO-Volts": "4.5000", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "555 Shutter-IOChannel": "Dev1/ao13", "WaitInterval": null, "520 Shutter-Sequenceable": "1", "YPositionUm": -13012.300000000001, "445-State": "0", "445-DA Device": "445 Shutter", "PRIME-CircularBufferAutoSize": "ON", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "555 AO-TriggerSampleRate": "100000", "445 Shutter-Volts": "0.0000", "XLIGHT Hub-Port": "COM3", "Core-AutoFocus": "", "COM4-Handshaking": "Off", "528 Shutter-MinVolts": "0.0000", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "445 Shutter-Name": "AnalogIO", "528 Shutter-Sequenceable": "1", "XYStage-TransposeMirrorX": "0", "XYStage-TransposeMirrorY": "0", "PRIME-ReadoutRate": "100MHz 16bit", "XYStage-Name": "Stage", "640 AO-SupportsTriggering": "0", "640-Name": "DA Shutter", "640 Shutter-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "640 AO-Block voltage": "false", "445 Shutter-Sequenceable": "1", "470 AO-MaxVolts": "5.0000", "COM4-BaudRate": "9600", "470-Description": "DA device that is used as a shutter", "520 Shutter-Description": "NI DAC", "PRIME-CCDTemperature": "-14.9200", "Time": "2019-12-17 03:59:53 -0500", "640 AO-TriggerSequenceLength": "1000", "470 AO-Name": "AnalogIO", "XLIGHT Disk slider-Label": "Disk Pos 40um", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "Binning": "1x1", "TE2000-ModelType": "1", "COM3-AnswerTimeout": "500.0000", "555 Shutter-SupportsTriggering": "0", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "TE2000-Port": "COM4", "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Description": "NI DAC", "COM4-AnswerTimeout": "500.0000", "COM1-DelayBetweenCharsMs": "0.0000", "FilterBlock-Label": "Confocal", "445 AO-Name": "AnalogIO", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-Timing-ReadoutTimeNs": "24672000.0000", "528 AO-Demo": "No", "520 AO-Sequenceable": "1", "555-Description": "DA device that is used as a shutter", "405-DA Device": "405 Shutter", "405 AO-TriggerSequenceLength": "1000", "640 AO-MinVolts": "0.0000", "640 Shutter-Block voltage": "false", "FrameIndex": 0, "PRIME-Port": "CMOS", "555 AO-MinVolts": "0.0000", "COM3-Description": "Serial port driver (boost:asio)", "528 AO-Description": "NI DAC", "555 AO-IOChannel": "Dev1/ao6", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "555 AO-TriggerInputLine": "/Dev1/PFI0", "PRIME-ActualInterval-ms": "84.6200", "LudlController-Port": "COM9", "445 Shutter-Demo": "No", "PRIME-Trigger-Expose Out-Mux": "1", "SliceIndex": 1, "PRIME-CCDTemperatureSetPoint": "-15.0000", "405 Shutter-MinVolts": "0.0000", "PRIME-ExposeOutMode": "First Row", "AxisPositions": null, "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Volts": "0.0000", "528-Name": "DA Shutter", "555-State": "0", "XLIGHT Dichroic-Label": "Di03-R635-t1", "445 AO-Sequenceable": "1", "405-Name": "DA Shutter", "405 AO-IOChannel": "Dev1/ao0", "Core-Camera": "PRIME", "Focus-Name": "Focus", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "405 Shutter-IOChannel": "Dev1/ao8", "640 AO-Demo": "No", "640 Shutter-SupportsTriggering": "0", "445 AO-Demo": "No", "555-DA Device": "555 Shutter", "470 Shutter-Description": "NI DAC", "528 AO-Volts": "0.0000", "COM1-DataBits": "8", "Vincent-D3-Address": "x", "520 AO-SupportsTriggering": "0", "405-Description": "DA device that is used as a shutter", "640 Shutter-MinVolts": "0.0000", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "405 AO-Name": "AnalogIO", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "640 AO-TriggerInputLine": "/Dev1/PFI0", "405 AO-TriggerInputLine": "/Dev1/PFI0", "640-Description": "DA device that is used as a shutter", "445 Shutter-TriggerSampleRate": "100000", "470 Shutter-TriggerSequenceLength": "1000", "COM1-StopBits": "1", "520 Shutter-SupportsTriggering": "0", "405-State": "0", "Core-Initialize": "1", "XLIGHT Emission Wheel-State": "3", "520 AO-Description": "NI DAC", "528 AO-TriggerInputLine": "/Dev1/PFI0", "445 Shutter-MaxVolts": "5.0000", "COM9-Handshaking": "Off", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "XLIGHT Spin motor-Label": "On (spinning)", "PRIME-PP 5 ENABLED": "No", "UUID": "b6bd926c-dd8f-49eb-beb5-6614a04e7dd8", "PRIME-TriggerMode": "Internal Trigger", "COM1-Handshaking": "Off", "PRIME-Timing-PostTriggerDelayNs": "49344000.0000", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "Focus-Position": "16732.9000", "528 Shutter-Block voltage": "false", "Channel": "Il-C", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "555 Shutter-Demo": "No", "405 Shutter-Demo": "No", "528 AO-Name": "AnalogIO", "520-State": "0", "Core-Galvo": "", "405 Shutter-TriggerSampleRate": "100000", "Focus-FocusStepSize_(nm)": "100", "PRIME-PixelType": "16bit", "COM4-DataBits": "8", "470 AO-IOChannel": "Dev1/ao2", "520 AO-MinVolts": "0.0000", "COM9-Parity": "None", "PRIME-CircularBufferEnabled": "ON", "PRIME-PP 6 Reset": "No", "640 AO-Description": "NI DAC", "Source": "PRIME", "445 AO-MinVolts": "0.0000", "PIEZO-Name": "Stage", "445 AO-Description": "NI DAC", "PRIME-CircularBufferFrameCount": "46", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "520 AO-Volts": "0.0000", "528 Shutter-TriggerSampleRate": "100000", "XPositionUm": 23269.100000000002, "OpticalPath-State": "4", "PRIME-PP 3 THRESHOLD": "80", "PRIME-PVCAM Version": "3.7.1", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Gain": "1-HDR", "640 Shutter-TriggerSequenceLength": "1000", "528 AO-SupportsTriggering": "0", "445 AO-TriggerInputLine": "/Dev1/PFI0", "OpticalPath-Name": "OpticalPath", "PRIME-PP 4 ENABLED": "Yes", "PRIME-PVCAM Adapter Version": "1.3.29", "COM3-Name": "COM3", "405 AO-MaxVolts": "5.0000", "520 Shutter-Block voltage": "false", "PRIME-Timing-ClearingTimeNs": "49344000.0000", "Core-Focus": "PIEZO", "528 AO-Block voltage": "false", "TE2000-Description": "Nikon TE2000 hub", "528 AO-MinVolts": "0.0000", "COM4-Parity": "None", "Summary": { "Slices": 11, "ChContrastMin": [ 0, 0, 0, 0, 0, 0 ], "PixelAspect": 1, "SlicesFirst": true, "Interval_ms": 0, "Time": "2019-12-17 03:59:33 -0500", "Prefix": "seq_7", "InitialPositionList": [ { "Label": "Pos0", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [149.91], "Focus": [16657.7], "XYStage": [ 71629.7, 19152.9 ] }, "GridColumnIndex": 0 }, { "Label": "Pos1", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [190.82], "Focus": [16485.4], "XYStage": [ 65702.3, 19396.600000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos2", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [194.69], "Focus": [16446.8], "XYStage": [ 59880.5, 19007.8 ] }, "GridColumnIndex": 0 }, { "Label": "Pos3", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [145.04], "Focus": [16446.6], "XYStage": [ 53616.9, 18818.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos4", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [169.57500000000002], "Focus": [16446.5], "XYStage": [ 47662.8, 18817.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos5", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [131.805], "Focus": [16446.4], "XYStage": [ 41918.8, 18298.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos6", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [113.145], "Focus": [16446.5], "XYStage": [ 36129.9, 18895.8 ] }, "GridColumnIndex": 0 }, { "Label": "Pos7", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [141.005], "Focus": [16446.6], "XYStage": [ 30299.100000000002, 18895.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos8", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [140.97], "Focus": [16446.5], "XYStage": [ 23952.100000000002, 19156 ] }, "GridColumnIndex": 0 }, { "Label": "Pos9", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [184.57500000000002], "Focus": [16677.9], "XYStage": [ 72866.8, -14809.300000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos10", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [167.95000000000002], "Focus": [16557.9], "XYStage": [ 66515.6, -14371.300000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos11", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [168], "Focus": [16618.9], "XYStage": [ 59724.3, -13964.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos12", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [203.205], "Focus": [16779.6], "XYStage": [ 53862.3, -13494.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos13", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [203.20000000000002], "Focus": [16749], "XYStage": [ 48108.3, -13805.400000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos14", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [203.19], "Focus": [16675.9], "XYStage": [ 41645, -13804.900000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos15", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [203.21], "Focus": [16675.7], "XYStage": [ 35352.6, -13805.6 ] }, "GridColumnIndex": 0 }, { "Label": "Pos16", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [203.225], "Focus": [16675.8], "XYStage": [ 29298, -13447.400000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos17", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [203.215], "Focus": [16675.8], "XYStage": [ 23269.100000000002, -13012.300000000001 ] }, "GridColumnIndex": 0 } ], "z-step_um": 10, "GridRow": 0, "BitDepth": 16, "Depth": 2, "Channels": 6, "Source": "Micro-Manager", "ChColors": [ -16711681, -256, -65281, -1, -1, -16776961 ], "MetadataVersion": 10, "KeepShutterOpenChannels": true, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536, 65536 ], "CustomIntervals_ms": [], "Height": 1200, "MicroManagerVersion": "1.4.23 20180219", "GridColumn": 0, "UUID": "3ad8801b-5699-40d6-813b-4ad840863e38", "PixelType": "GRAY16", "Width": 1200, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\Yan\\20191216-YC113-S11S12-miseq", "Frames": 1, "Comment": "", "UserName": "Nikon", "PixelSize_um": 1.1, "ROI": [ 0, 0, 1200, 1200 ], "Date": "2019-12-17", "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC", "P-DAPI-high" ], "KeepShutterOpenSlices": true, "IJType": 1, "ComputerName": "ZadorConfocal2", "Positions": 18, "PositionIndex": 17, "CameraTimeout": 20000, "TimeFirst": false }, "640 AO-Name": "AnalogIO", "Vincent-D3-Command": "Close" }, "FrameKey-0-3-2": { "COM4-StopBits": "1", "528 AO-MaxVolts": "5.0000", "XLIGHT Excitation Wheel-State": "1", "470 Shutter-Name": "AnalogIO", "Core-Shutter": "640", "555 AO-Demo": "No", "XLIGHT Dichroic-State": "2", "XYStage-StepSize-Y": "0.1000", "ZPositionUm": 173.215, "XYStage-StepSize-X": "0.1000", "PRIME-FirmwareVersion": "20.28", "PRIME-ClearCycles": "2", "520-Name": "DA Shutter", "405 Shutter-Name": "AnalogIO", "PIEZO-StepSize": "0.0050", "PRIME-ClearMode": "Pre-Exposure", "470 AO-MinVolts": "0.0000", "405 AO-SupportsTriggering": "1", "CameraChannelIndex": 0, "XYStage-StartSpeed": "500.0000", "COM9-StopBits": "2", "405 AO-Description": "NI DAC", "COM4-DelayBetweenCharsMs": "0.0000", "445 AO-Block voltage": "false", "520 AO-TriggerInputLine": "/Dev1/PFI0", "555 AO-Description": "NI DAC", "520 AO-TriggerSampleRate": "100000", "COM4-Verbose": "1", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM4-Name": "COM4", "405 Shutter-Volts": "0.0000", "Vincent-D3-Port": "COM1", "PRIME-PP 4 MIN ADU AFFECTED": "200", "470 Shutter-MinVolts": "0.0000", "PRIME-PP 3 ENABLED": "Yes", "470 AO-Description": "NI DAC", "PRIME-Description": "PVCAM API device adapter", "520 Shutter-MaxVolts": "5.0000", "520 Shutter-Volts": "0.0000", "528 AO-Sequenceable": "1", "PRIME-Binning": "1x1", "555 Shutter-MaxVolts": "5.0000", "528 Shutter-TriggerSequenceLength": "1000", "405 Shutter-Block voltage": "false", "XLIGHT Spin motor-State": "1", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "Vincent-D3-Time to open (ms)": "35.0000", "Vincent-D3-Time to close (ms)": "35.0000", "445 AO-TriggerSampleRate": "100000", "640 Shutter-IOChannel": "Dev1/ao14", "555 AO-Sequenceable": "1", "520 AO-Name": "AnalogIO", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "470 AO-Block voltage": "false", "COM1-Verbose": "1", "COM9-AnswerTimeout": "10000.0000", "528 Shutter-Name": "AnalogIO", "PRIME-Trigger Timeout (secs)": "10", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "NextFrame": 0, "470 AO-TriggerSequenceLength": "1000", "PRIME-TransposeXY": "0", "445 AO-TriggerSequenceLength": "1000", "COM1-Name": "COM1", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "COM9-DataBits": "8", "COM1-AnswerTimeout": "500.0000", "Camera": "", "TE2000-Version": "V2.50-V2.50-V2.10", "528 Shutter-Volts": "0.0000", "XLIGHT Hub-Name": "XLIGHT Hub", "PIEZO-Description": "Ludl stage driver adapter", "PositionName": "Pos17", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSampleRate": "100000", "PRIME-FanSpeedSetpoint": "High", "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "405 AO-TriggerSampleRate": "100000", "470 Shutter-Block voltage": "false", "470-State": "0", "COM3-Parity": "None", "405 AO-Volts": "0.0000", "PRIME-TransposeMirrorY": "0", "PRIME-ChipName": "GS144BSI", "470 AO-Volts": "0.0000", "PRIME-TransposeMirrorX": "0", "445 Shutter-SupportsTriggering": "0", "470 Shutter-SupportsTriggering": "0", "XLIGHT Hub-Description": "XLIGHT hub", "PRIME-PreampOffLimit": "10000", "470 AO-Sequenceable": "1", "528 Shutter-MaxVolts": "5.0000", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "Core-XYStage": "XYStage", "470 AO-TriggerSampleRate": "100000", "PRIME-Timing-PreTriggerDelayNs": "24672000.0000", "528-DA Device": "528 Shutter", "Focus-Description": "Nikon TE2000 focus adapter", "FilterBlock-State": "5", "520 AO-Demo": "No", "COM3-DelayBetweenCharsMs": "0.0000", "470 Shutter-Volts": "0.0000", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "PRIME-PP 2 THRESHOLD": "125", "PRIME-PP 4 THRESHOLD": "75", "XYStage-Acceleration": "4.0000", "470 Shutter-Demo": "No", "PRIME-Name": "PMPCIECam00", "640-DA Device": "640 Shutter", "405 AO-Block voltage": "false", "ElapsedTime-ms": 736860, "PRIME-Offset": "100", "445 AO-IOChannel": "Dev1/ao1", "640 AO-MaxVolts": "5.0000", "PositionIndex": 17, "PRIME-PP 1 ENABLED": "Yes", "COM1-BaudRate": "9600", "640-State": "1", "COM3-Verbose": "1", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "LudlController-TransmissionDelay": "0", "PRIME-FullWellCapacity": "62000", "PRIME-SMARTStreamingEnabled": "No", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "640 Shutter-Description": "NI DAC", "528-Description": "DA device that is used as a shutter", "COM4-Description": "Serial port driver (boost:asio)", "405 Shutter-Description": "NI DAC", "TE2000-Name": "TE2000", "528 Shutter-Description": "NI DAC", "COM9-Description": "Serial port driver (boost:asio)", "LudlController-Name": "LudlController", "445 Shutter-TriggerSequenceLength": "1000", "PRIME-SerialNumber": "A18B203008", "445 AO-Volts": "0.0000", "PRIME-X-dimension": "1200", "PRIME-ShutterCloseDelay": "0", "XLIGHT Disk slider-State": "2", "Position": "Default", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 AO-IOChannel": "Dev1/ao5", "520 AO-IOChannel": "Dev1/ao4", "528 AO-TriggerSequenceLength": "1000", "405 Shutter-MaxVolts": "5.0000", "470-DA Device": "470 Shutter", "PRIME-FTCapable": "No", "Core-ChannelGroup": "Channels", "555 AO-SupportsTriggering": "0", "PRIME-Y-dimension": "1200", "Frame": 0, "555 Shutter-Sequenceable": "1", "PRIME-Timing-ExposureTimeNs": "499998000.0000", "Core-ImageProcessor": "", "528-State": "0", "520 AO-Block voltage": "false", "XLIGHT Excitation Wheel-Label": "QUAD", "640 Shutter-Demo": "No", "COM3-StopBits": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "520 Shutter-IOChannel": "Dev1/ao11", "Width": 1200, "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-SupportsTriggering": "0", "640 AO-TriggerSampleRate": "100000", "520-Description": "DA device that is used as a shutter", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "405 Shutter-Sequenceable": "1", "405 Shutter-SupportsTriggering": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-TriggerSampleRate": "100000", "640 Shutter-MaxVolts": "5.0000", "555 AO-MaxVolts": "5.0000", "520 AO-TriggerSequenceLength": "1000", "COM3-BaudRate": "9600", "COM9-BaudRate": "9600", "Vincent-D3-Channel #": "Ch. #1", "PRIME-PMode": "Normal", "OpticalPath-Label": "Left(100)", "BitDepth": 16, "555 AO-TriggerSequenceLength": "1000", "445 Shutter-Description": "NI DAC", "405 AO-Sequenceable": "1", "PRIME-AcquisitionMethod": "Callbacks", "555 AO-Name": "AnalogIO", "PRIME-ShutterMode": "Pre-Exposure", "COM3-Handshaking": "Off", "445 AO-SupportsTriggering": "0", "ROI": "0-0-1200-1200", "COM9-Verbose": "1", "PRIME-Color": "OFF", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "PRIME-ShutterOpenDelay": "0", "COM1-Parity": "None", "Slice": 2, "SlicePosition": -30, "COM9-DelayBetweenCharsMs": "0.0000", "555-Name": "DA Shutter", "PIEZO-LudlSingleAxisName": "B", "XLIGHT Emission Wheel-Label": "725/40 (C)", "640 Shutter-Name": "AnalogIO", "640 AO-Sequenceable": "1", "520 Shutter-Demo": "No", "528 Shutter-Demo": "No", "470-Name": "DA Shutter", "520-DA Device": "520 Shutter", "XYStage-Description": "Ludl XY stage driver adapter", "445 Shutter-MinVolts": "0.0000", "528 AO-TriggerSampleRate": "100000", "405 AO-MinVolts": "0.0000", "405 Shutter-TriggerSequenceLength": "1000", "Height": 1200, "555 AO-Block voltage": "false", "640 Shutter-Volts": "5.0000", "PixelType": "GRAY16", "445 Shutter-Block voltage": "false", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "520 Shutter-TriggerSequenceLength": "1000", "COM9-Name": "COM9", "Core-SLM": "", "555 AO-Volts": "0.0000", "555 Shutter-Block voltage": "false", "XYStage-Speed": "1800.0000", "520 AO-MaxVolts": "5.0000", "528 Shutter-SupportsTriggering": "0", "COM1-Description": "Serial port driver (boost:asio)", "PRIME-MetadataEnabled": "No", "520 Shutter-MinVolts": "0.0000", "470 Shutter-Sequenceable": "1", "PRIME-PreampDelay": "0", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-MinVolts": "0.0000", "ChannelIndex": 3, "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Core-AutoShutter": "1", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "Exposure-ms": 520, "PRIME-PP 2 ENABLED": "Yes", "640 Shutter-TriggerSampleRate": "100000", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "470 Shutter-IOChannel": "Dev1/ao10", "405 AO-Demo": "No", "PixelSizeUm": 1.1, "470 AO-Demo": "No", "470 Shutter-MaxVolts": "5.0000", "Vincent-D3-Name": "Vincent-D3", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FileName": "img_000000000_Il-C_002.tif", "LudlController-Reset": "Operate", "PRIME-Exposure": "520.00", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "445-Name": "DA Shutter", "555 Shutter-Name": "AnalogIO", "520 Shutter-TriggerSampleRate": "100000", "445 Shutter-IOChannel": "Dev1/ao9", "PRIME-TransposeCorrection": "0", "XYStage-StepSize": "0.1000", "445-Description": "DA device that is used as a shutter", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 AO-IOChannel": "Dev1/ao7", "520 Shutter-Name": "AnalogIO", "COM3-DataBits": "8", "640 AO-Volts": "4.5000", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "555 Shutter-IOChannel": "Dev1/ao13", "WaitInterval": null, "520 Shutter-Sequenceable": "1", "YPositionUm": -13012.300000000001, "445-State": "0", "445-DA Device": "445 Shutter", "PRIME-CircularBufferAutoSize": "ON", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "555 AO-TriggerSampleRate": "100000", "445 Shutter-Volts": "0.0000", "XLIGHT Hub-Port": "COM3", "Core-AutoFocus": "", "COM4-Handshaking": "Off", "528 Shutter-MinVolts": "0.0000", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "445 Shutter-Name": "AnalogIO", "528 Shutter-Sequenceable": "1", "XYStage-TransposeMirrorX": "0", "XYStage-TransposeMirrorY": "0", "PRIME-ReadoutRate": "100MHz 16bit", "XYStage-Name": "Stage", "640 AO-SupportsTriggering": "0", "640-Name": "DA Shutter", "640 Shutter-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "640 AO-Block voltage": "false", "445 Shutter-Sequenceable": "1", "470 AO-MaxVolts": "5.0000", "COM4-BaudRate": "9600", "470-Description": "DA device that is used as a shutter", "520 Shutter-Description": "NI DAC", "PRIME-CCDTemperature": "-14.9200", "Time": "2019-12-17 03:59:53 -0500", "640 AO-TriggerSequenceLength": "1000", "470 AO-Name": "AnalogIO", "XLIGHT Disk slider-Label": "Disk Pos 40um", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "Binning": "1x1", "TE2000-ModelType": "1", "COM3-AnswerTimeout": "500.0000", "555 Shutter-SupportsTriggering": "0", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "TE2000-Port": "COM4", "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Description": "NI DAC", "COM4-AnswerTimeout": "500.0000", "COM1-DelayBetweenCharsMs": "0.0000", "FilterBlock-Label": "Confocal", "445 AO-Name": "AnalogIO", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-Timing-ReadoutTimeNs": "24672000.0000", "528 AO-Demo": "No", "520 AO-Sequenceable": "1", "555-Description": "DA device that is used as a shutter", "405-DA Device": "405 Shutter", "405 AO-TriggerSequenceLength": "1000", "640 AO-MinVolts": "0.0000", "640 Shutter-Block voltage": "false", "FrameIndex": 0, "PRIME-Port": "CMOS", "555 AO-MinVolts": "0.0000", "COM3-Description": "Serial port driver (boost:asio)", "528 AO-Description": "NI DAC", "555 AO-IOChannel": "Dev1/ao6", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "555 AO-TriggerInputLine": "/Dev1/PFI0", "PRIME-ActualInterval-ms": "84.6200", "LudlController-Port": "COM9", "445 Shutter-Demo": "No", "PRIME-Trigger-Expose Out-Mux": "1", "SliceIndex": 2, "PRIME-CCDTemperatureSetPoint": "-15.0000", "405 Shutter-MinVolts": "0.0000", "PRIME-ExposeOutMode": "First Row", "AxisPositions": null, "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Volts": "0.0000", "528-Name": "DA Shutter", "555-State": "0", "XLIGHT Dichroic-Label": "Di03-R635-t1", "445 AO-Sequenceable": "1", "405-Name": "DA Shutter", "405 AO-IOChannel": "Dev1/ao0", "Core-Camera": "PRIME", "Focus-Name": "Focus", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "405 Shutter-IOChannel": "Dev1/ao8", "640 AO-Demo": "No", "640 Shutter-SupportsTriggering": "0", "445 AO-Demo": "No", "555-DA Device": "555 Shutter", "470 Shutter-Description": "NI DAC", "528 AO-Volts": "0.0000", "COM1-DataBits": "8", "Vincent-D3-Address": "x", "520 AO-SupportsTriggering": "0", "405-Description": "DA device that is used as a shutter", "640 Shutter-MinVolts": "0.0000", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "405 AO-Name": "AnalogIO", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "640 AO-TriggerInputLine": "/Dev1/PFI0", "405 AO-TriggerInputLine": "/Dev1/PFI0", "640-Description": "DA device that is used as a shutter", "445 Shutter-TriggerSampleRate": "100000", "470 Shutter-TriggerSequenceLength": "1000", "COM1-StopBits": "1", "520 Shutter-SupportsTriggering": "0", "405-State": "0", "Core-Initialize": "1", "XLIGHT Emission Wheel-State": "3", "520 AO-Description": "NI DAC", "528 AO-TriggerInputLine": "/Dev1/PFI0", "445 Shutter-MaxVolts": "5.0000", "COM9-Handshaking": "Off", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "XLIGHT Spin motor-Label": "On (spinning)", "PRIME-PP 5 ENABLED": "No", "UUID": "96668957-1cf6-4dc2-9600-618e4c26f9aa", "PRIME-TriggerMode": "Internal Trigger", "COM1-Handshaking": "Off", "PRIME-Timing-PostTriggerDelayNs": "49344000.0000", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "Focus-Position": "16732.9000", "528 Shutter-Block voltage": "false", "Channel": "Il-C", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "555 Shutter-Demo": "No", "405 Shutter-Demo": "No", "528 AO-Name": "AnalogIO", "520-State": "0", "Core-Galvo": "", "405 Shutter-TriggerSampleRate": "100000", "Focus-FocusStepSize_(nm)": "100", "PRIME-PixelType": "16bit", "COM4-DataBits": "8", "470 AO-IOChannel": "Dev1/ao2", "520 AO-MinVolts": "0.0000", "COM9-Parity": "None", "PRIME-CircularBufferEnabled": "ON", "PRIME-PP 6 Reset": "No", "640 AO-Description": "NI DAC", "Source": "PRIME", "445 AO-MinVolts": "0.0000", "PIEZO-Name": "Stage", "445 AO-Description": "NI DAC", "PRIME-CircularBufferFrameCount": "46", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "520 AO-Volts": "0.0000", "528 Shutter-TriggerSampleRate": "100000", "XPositionUm": 23269.100000000002, "OpticalPath-State": "4", "PRIME-PP 3 THRESHOLD": "80", "PRIME-PVCAM Version": "3.7.1", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Gain": "1-HDR", "640 Shutter-TriggerSequenceLength": "1000", "528 AO-SupportsTriggering": "0", "445 AO-TriggerInputLine": "/Dev1/PFI0", "OpticalPath-Name": "OpticalPath", "PRIME-PP 4 ENABLED": "Yes", "PRIME-PVCAM Adapter Version": "1.3.29", "COM3-Name": "COM3", "405 AO-MaxVolts": "5.0000", "520 Shutter-Block voltage": "false", "PRIME-Timing-ClearingTimeNs": "49344000.0000", "Core-Focus": "PIEZO", "528 AO-Block voltage": "false", "TE2000-Description": "Nikon TE2000 hub", "528 AO-MinVolts": "0.0000", "COM4-Parity": "None", "Summary": { "Slices": 11, "ChContrastMin": [ 0, 0, 0, 0, 0, 0 ], "PixelAspect": 1, "SlicesFirst": true, "Interval_ms": 0, "Time": "2019-12-17 03:59:33 -0500", "Prefix": "seq_7", "InitialPositionList": [ { "Label": "Pos0", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [149.91], "Focus": [16657.7], "XYStage": [ 71629.7, 19152.9 ] }, "GridColumnIndex": 0 }, { "Label": "Pos1", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [190.82], "Focus": [16485.4], "XYStage": [ 65702.3, 19396.600000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos2", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [194.69], "Focus": [16446.8], "XYStage": [ 59880.5, 19007.8 ] }, "GridColumnIndex": 0 }, { "Label": "Pos3", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [145.04], "Focus": [16446.6], "XYStage": [ 53616.9, 18818.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos4", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [169.57500000000002], "Focus": [16446.5], "XYStage": [ 47662.8, 18817.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos5", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [131.805], "Focus": [16446.4], "XYStage": [ 41918.8, 18298.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos6", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [113.145], "Focus": [16446.5], "XYStage": [ 36129.9, 18895.8 ] }, "GridColumnIndex": 0 }, { "Label": "Pos7", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [141.005], "Focus": [16446.6], "XYStage": [ 30299.100000000002, 18895.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos8", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [140.97], "Focus": [16446.5], "XYStage": [ 23952.100000000002, 19156 ] }, "GridColumnIndex": 0 }, { "Label": "Pos9", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [184.57500000000002], "Focus": [16677.9], "XYStage": [ 72866.8, -14809.300000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos10", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [167.95000000000002], "Focus": [16557.9], "XYStage": [ 66515.6, -14371.300000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos11", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [168], "Focus": [16618.9], "XYStage": [ 59724.3, -13964.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos12", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [203.205], "Focus": [16779.6], "XYStage": [ 53862.3, -13494.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos13", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [203.20000000000002], "Focus": [16749], "XYStage": [ 48108.3, -13805.400000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos14", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [203.19], "Focus": [16675.9], "XYStage": [ 41645, -13804.900000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos15", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [203.21], "Focus": [16675.7], "XYStage": [ 35352.6, -13805.6 ] }, "GridColumnIndex": 0 }, { "Label": "Pos16", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [203.225], "Focus": [16675.8], "XYStage": [ 29298, -13447.400000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos17", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [203.215], "Focus": [16675.8], "XYStage": [ 23269.100000000002, -13012.300000000001 ] }, "GridColumnIndex": 0 } ], "z-step_um": 10, "GridRow": 0, "BitDepth": 16, "Depth": 2, "Channels": 6, "Source": "Micro-Manager", "ChColors": [ -16711681, -256, -65281, -1, -1, -16776961 ], "MetadataVersion": 10, "KeepShutterOpenChannels": true, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536, 65536 ], "CustomIntervals_ms": [], "Height": 1200, "MicroManagerVersion": "1.4.23 20180219", "GridColumn": 0, "UUID": "3ad8801b-5699-40d6-813b-4ad840863e38", "PixelType": "GRAY16", "Width": 1200, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\Yan\\20191216-YC113-S11S12-miseq", "Frames": 1, "Comment": "", "UserName": "Nikon", "PixelSize_um": 1.1, "ROI": [ 0, 0, 1200, 1200 ], "Date": "2019-12-17", "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC", "P-DAPI-high" ], "KeepShutterOpenSlices": true, "IJType": 1, "ComputerName": "ZadorConfocal2", "Positions": 18, "PositionIndex": 17, "CameraTimeout": 20000, "TimeFirst": false }, "640 AO-Name": "AnalogIO", "Vincent-D3-Command": "Close" }, "FrameKey-0-3-3": { "COM4-StopBits": "1", "528 AO-MaxVolts": "5.0000", "XLIGHT Excitation Wheel-State": "1", "470 Shutter-Name": "AnalogIO", "Core-Shutter": "640", "555 AO-Demo": "No", "XLIGHT Dichroic-State": "2", "XYStage-StepSize-Y": "0.1000", "ZPositionUm": 183.215, "XYStage-StepSize-X": "0.1000", "PRIME-FirmwareVersion": "20.28", "PRIME-ClearCycles": "2", "520-Name": "DA Shutter", "405 Shutter-Name": "AnalogIO", "PIEZO-StepSize": "0.0050", "PRIME-ClearMode": "Pre-Exposure", "470 AO-MinVolts": "0.0000", "405 AO-SupportsTriggering": "1", "CameraChannelIndex": 0, "XYStage-StartSpeed": "500.0000", "COM9-StopBits": "2", "405 AO-Description": "NI DAC", "COM4-DelayBetweenCharsMs": "0.0000", "445 AO-Block voltage": "false", "520 AO-TriggerInputLine": "/Dev1/PFI0", "555 AO-Description": "NI DAC", "520 AO-TriggerSampleRate": "100000", "COM4-Verbose": "1", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM4-Name": "COM4", "405 Shutter-Volts": "0.0000", "Vincent-D3-Port": "COM1", "PRIME-PP 4 MIN ADU AFFECTED": "200", "470 Shutter-MinVolts": "0.0000", "PRIME-PP 3 ENABLED": "Yes", "470 AO-Description": "NI DAC", "PRIME-Description": "PVCAM API device adapter", "520 Shutter-MaxVolts": "5.0000", "520 Shutter-Volts": "0.0000", "528 AO-Sequenceable": "1", "PRIME-Binning": "1x1", "555 Shutter-MaxVolts": "5.0000", "528 Shutter-TriggerSequenceLength": "1000", "405 Shutter-Block voltage": "false", "XLIGHT Spin motor-State": "1", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "Vincent-D3-Time to open (ms)": "35.0000", "Vincent-D3-Time to close (ms)": "35.0000", "445 AO-TriggerSampleRate": "100000", "640 Shutter-IOChannel": "Dev1/ao14", "555 AO-Sequenceable": "1", "520 AO-Name": "AnalogIO", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "470 AO-Block voltage": "false", "COM1-Verbose": "1", "COM9-AnswerTimeout": "10000.0000", "528 Shutter-Name": "AnalogIO", "PRIME-Trigger Timeout (secs)": "10", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "NextFrame": 0, "470 AO-TriggerSequenceLength": "1000", "PRIME-TransposeXY": "0", "445 AO-TriggerSequenceLength": "1000", "COM1-Name": "COM1", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "COM9-DataBits": "8", "COM1-AnswerTimeout": "500.0000", "Camera": "", "TE2000-Version": "V2.50-V2.50-V2.10", "528 Shutter-Volts": "0.0000", "XLIGHT Hub-Name": "XLIGHT Hub", "PIEZO-Description": "Ludl stage driver adapter", "PositionName": "Pos17", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSampleRate": "100000", "PRIME-FanSpeedSetpoint": "High", "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "405 AO-TriggerSampleRate": "100000", "470 Shutter-Block voltage": "false", "470-State": "0", "COM3-Parity": "None", "405 AO-Volts": "0.0000", "PRIME-TransposeMirrorY": "0", "PRIME-ChipName": "GS144BSI", "470 AO-Volts": "0.0000", "PRIME-TransposeMirrorX": "0", "445 Shutter-SupportsTriggering": "0", "470 Shutter-SupportsTriggering": "0", "XLIGHT Hub-Description": "XLIGHT hub", "PRIME-PreampOffLimit": "10000", "470 AO-Sequenceable": "1", "528 Shutter-MaxVolts": "5.0000", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "Core-XYStage": "XYStage", "470 AO-TriggerSampleRate": "100000", "PRIME-Timing-PreTriggerDelayNs": "24672000.0000", "528-DA Device": "528 Shutter", "Focus-Description": "Nikon TE2000 focus adapter", "FilterBlock-State": "5", "520 AO-Demo": "No", "COM3-DelayBetweenCharsMs": "0.0000", "470 Shutter-Volts": "0.0000", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "PRIME-PP 2 THRESHOLD": "125", "PRIME-PP 4 THRESHOLD": "75", "XYStage-Acceleration": "4.0000", "470 Shutter-Demo": "No", "PRIME-Name": "PMPCIECam00", "640-DA Device": "640 Shutter", "405 AO-Block voltage": "false", "ElapsedTime-ms": 737626, "PRIME-Offset": "100", "445 AO-IOChannel": "Dev1/ao1", "640 AO-MaxVolts": "5.0000", "PositionIndex": 17, "PRIME-PP 1 ENABLED": "Yes", "COM1-BaudRate": "9600", "640-State": "1", "COM3-Verbose": "1", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "LudlController-TransmissionDelay": "0", "PRIME-FullWellCapacity": "62000", "PRIME-SMARTStreamingEnabled": "No", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "640 Shutter-Description": "NI DAC", "528-Description": "DA device that is used as a shutter", "COM4-Description": "Serial port driver (boost:asio)", "405 Shutter-Description": "NI DAC", "TE2000-Name": "TE2000", "528 Shutter-Description": "NI DAC", "COM9-Description": "Serial port driver (boost:asio)", "LudlController-Name": "LudlController", "445 Shutter-TriggerSequenceLength": "1000", "PRIME-SerialNumber": "A18B203008", "445 AO-Volts": "0.0000", "PRIME-X-dimension": "1200", "PRIME-ShutterCloseDelay": "0", "XLIGHT Disk slider-State": "2", "Position": "Default", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 AO-IOChannel": "Dev1/ao5", "520 AO-IOChannel": "Dev1/ao4", "528 AO-TriggerSequenceLength": "1000", "405 Shutter-MaxVolts": "5.0000", "470-DA Device": "470 Shutter", "PRIME-FTCapable": "No", "Core-ChannelGroup": "Channels", "555 AO-SupportsTriggering": "0", "PRIME-Y-dimension": "1200", "Frame": 0, "555 Shutter-Sequenceable": "1", "PRIME-Timing-ExposureTimeNs": "499998000.0000", "Core-ImageProcessor": "", "528-State": "0", "520 AO-Block voltage": "false", "XLIGHT Excitation Wheel-Label": "QUAD", "640 Shutter-Demo": "No", "COM3-StopBits": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "520 Shutter-IOChannel": "Dev1/ao11", "Width": 1200, "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-SupportsTriggering": "0", "640 AO-TriggerSampleRate": "100000", "520-Description": "DA device that is used as a shutter", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "405 Shutter-Sequenceable": "1", "405 Shutter-SupportsTriggering": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-TriggerSampleRate": "100000", "640 Shutter-MaxVolts": "5.0000", "555 AO-MaxVolts": "5.0000", "520 AO-TriggerSequenceLength": "1000", "COM3-BaudRate": "9600", "COM9-BaudRate": "9600", "Vincent-D3-Channel #": "Ch. #1", "PRIME-PMode": "Normal", "OpticalPath-Label": "Left(100)", "BitDepth": 16, "555 AO-TriggerSequenceLength": "1000", "445 Shutter-Description": "NI DAC", "405 AO-Sequenceable": "1", "PRIME-AcquisitionMethod": "Callbacks", "555 AO-Name": "AnalogIO", "PRIME-ShutterMode": "Pre-Exposure", "COM3-Handshaking": "Off", "445 AO-SupportsTriggering": "0", "ROI": "0-0-1200-1200", "COM9-Verbose": "1", "PRIME-Color": "OFF", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "PRIME-ShutterOpenDelay": "0", "COM1-Parity": "None", "Slice": 3, "SlicePosition": -20, "COM9-DelayBetweenCharsMs": "0.0000", "555-Name": "DA Shutter", "PIEZO-LudlSingleAxisName": "B", "XLIGHT Emission Wheel-Label": "725/40 (C)", "640 Shutter-Name": "AnalogIO", "640 AO-Sequenceable": "1", "520 Shutter-Demo": "No", "528 Shutter-Demo": "No", "470-Name": "DA Shutter", "520-DA Device": "520 Shutter", "XYStage-Description": "Ludl XY stage driver adapter", "445 Shutter-MinVolts": "0.0000", "528 AO-TriggerSampleRate": "100000", "405 AO-MinVolts": "0.0000", "405 Shutter-TriggerSequenceLength": "1000", "Height": 1200, "555 AO-Block voltage": "false", "640 Shutter-Volts": "5.0000", "PixelType": "GRAY16", "445 Shutter-Block voltage": "false", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "520 Shutter-TriggerSequenceLength": "1000", "COM9-Name": "COM9", "Core-SLM": "", "555 AO-Volts": "0.0000", "555 Shutter-Block voltage": "false", "XYStage-Speed": "1800.0000", "520 AO-MaxVolts": "5.0000", "528 Shutter-SupportsTriggering": "0", "COM1-Description": "Serial port driver (boost:asio)", "PRIME-MetadataEnabled": "No", "520 Shutter-MinVolts": "0.0000", "470 Shutter-Sequenceable": "1", "PRIME-PreampDelay": "0", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-MinVolts": "0.0000", "ChannelIndex": 3, "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Core-AutoShutter": "1", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "Exposure-ms": 520, "PRIME-PP 2 ENABLED": "Yes", "640 Shutter-TriggerSampleRate": "100000", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "470 Shutter-IOChannel": "Dev1/ao10", "405 AO-Demo": "No", "PixelSizeUm": 1.1, "470 AO-Demo": "No", "470 Shutter-MaxVolts": "5.0000", "Vincent-D3-Name": "Vincent-D3", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FileName": "img_000000000_Il-C_003.tif", "LudlController-Reset": "Operate", "PRIME-Exposure": "520.00", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "445-Name": "DA Shutter", "555 Shutter-Name": "AnalogIO", "520 Shutter-TriggerSampleRate": "100000", "445 Shutter-IOChannel": "Dev1/ao9", "PRIME-TransposeCorrection": "0", "XYStage-StepSize": "0.1000", "445-Description": "DA device that is used as a shutter", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 AO-IOChannel": "Dev1/ao7", "520 Shutter-Name": "AnalogIO", "COM3-DataBits": "8", "640 AO-Volts": "4.5000", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "555 Shutter-IOChannel": "Dev1/ao13", "WaitInterval": null, "520 Shutter-Sequenceable": "1", "YPositionUm": -13012.300000000001, "445-State": "0", "445-DA Device": "445 Shutter", "PRIME-CircularBufferAutoSize": "ON", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "555 AO-TriggerSampleRate": "100000", "445 Shutter-Volts": "0.0000", "XLIGHT Hub-Port": "COM3", "Core-AutoFocus": "", "COM4-Handshaking": "Off", "528 Shutter-MinVolts": "0.0000", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "445 Shutter-Name": "AnalogIO", "528 Shutter-Sequenceable": "1", "XYStage-TransposeMirrorX": "0", "XYStage-TransposeMirrorY": "0", "PRIME-ReadoutRate": "100MHz 16bit", "XYStage-Name": "Stage", "640 AO-SupportsTriggering": "0", "640-Name": "DA Shutter", "640 Shutter-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "640 AO-Block voltage": "false", "445 Shutter-Sequenceable": "1", "470 AO-MaxVolts": "5.0000", "COM4-BaudRate": "9600", "470-Description": "DA device that is used as a shutter", "520 Shutter-Description": "NI DAC", "PRIME-CCDTemperature": "-14.9200", "Time": "2019-12-17 03:59:54 -0500", "640 AO-TriggerSequenceLength": "1000", "470 AO-Name": "AnalogIO", "XLIGHT Disk slider-Label": "Disk Pos 40um", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "Binning": "1x1", "TE2000-ModelType": "1", "COM3-AnswerTimeout": "500.0000", "555 Shutter-SupportsTriggering": "0", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "TE2000-Port": "COM4", "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Description": "NI DAC", "COM4-AnswerTimeout": "500.0000", "COM1-DelayBetweenCharsMs": "0.0000", "FilterBlock-Label": "Confocal", "445 AO-Name": "AnalogIO", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-Timing-ReadoutTimeNs": "24672000.0000", "528 AO-Demo": "No", "520 AO-Sequenceable": "1", "555-Description": "DA device that is used as a shutter", "405-DA Device": "405 Shutter", "405 AO-TriggerSequenceLength": "1000", "640 AO-MinVolts": "0.0000", "640 Shutter-Block voltage": "false", "FrameIndex": 0, "PRIME-Port": "CMOS", "555 AO-MinVolts": "0.0000", "COM3-Description": "Serial port driver (boost:asio)", "528 AO-Description": "NI DAC", "555 AO-IOChannel": "Dev1/ao6", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "555 AO-TriggerInputLine": "/Dev1/PFI0", "PRIME-ActualInterval-ms": "84.6200", "LudlController-Port": "COM9", "445 Shutter-Demo": "No", "PRIME-Trigger-Expose Out-Mux": "1", "SliceIndex": 3, "PRIME-CCDTemperatureSetPoint": "-15.0000", "405 Shutter-MinVolts": "0.0000", "PRIME-ExposeOutMode": "First Row", "AxisPositions": null, "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Volts": "0.0000", "528-Name": "DA Shutter", "555-State": "0", "XLIGHT Dichroic-Label": "Di03-R635-t1", "445 AO-Sequenceable": "1", "405-Name": "DA Shutter", "405 AO-IOChannel": "Dev1/ao0", "Core-Camera": "PRIME", "Focus-Name": "Focus", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "405 Shutter-IOChannel": "Dev1/ao8", "640 AO-Demo": "No", "640 Shutter-SupportsTriggering": "0", "445 AO-Demo": "No", "555-DA Device": "555 Shutter", "470 Shutter-Description": "NI DAC", "528 AO-Volts": "0.0000", "COM1-DataBits": "8", "Vincent-D3-Address": "x", "520 AO-SupportsTriggering": "0", "405-Description": "DA device that is used as a shutter", "640 Shutter-MinVolts": "0.0000", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "405 AO-Name": "AnalogIO", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "640 AO-TriggerInputLine": "/Dev1/PFI0", "405 AO-TriggerInputLine": "/Dev1/PFI0", "640-Description": "DA device that is used as a shutter", "445 Shutter-TriggerSampleRate": "100000", "470 Shutter-TriggerSequenceLength": "1000", "COM1-StopBits": "1", "520 Shutter-SupportsTriggering": "0", "405-State": "0", "Core-Initialize": "1", "XLIGHT Emission Wheel-State": "3", "520 AO-Description": "NI DAC", "528 AO-TriggerInputLine": "/Dev1/PFI0", "445 Shutter-MaxVolts": "5.0000", "COM9-Handshaking": "Off", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "XLIGHT Spin motor-Label": "On (spinning)", "PRIME-PP 5 ENABLED": "No", "UUID": "6a442bdb-a4d0-4537-bcc4-64f60d198307", "PRIME-TriggerMode": "Internal Trigger", "COM1-Handshaking": "Off", "PRIME-Timing-PostTriggerDelayNs": "49344000.0000", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "Focus-Position": "16732.9000", "528 Shutter-Block voltage": "false", "Channel": "Il-C", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "555 Shutter-Demo": "No", "405 Shutter-Demo": "No", "528 AO-Name": "AnalogIO", "520-State": "0", "Core-Galvo": "", "405 Shutter-TriggerSampleRate": "100000", "Focus-FocusStepSize_(nm)": "100", "PRIME-PixelType": "16bit", "COM4-DataBits": "8", "470 AO-IOChannel": "Dev1/ao2", "520 AO-MinVolts": "0.0000", "COM9-Parity": "None", "PRIME-CircularBufferEnabled": "ON", "PRIME-PP 6 Reset": "No", "640 AO-Description": "NI DAC", "Source": "PRIME", "445 AO-MinVolts": "0.0000", "PIEZO-Name": "Stage", "445 AO-Description": "NI DAC", "PRIME-CircularBufferFrameCount": "46", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "520 AO-Volts": "0.0000", "528 Shutter-TriggerSampleRate": "100000", "XPositionUm": 23269.100000000002, "OpticalPath-State": "4", "PRIME-PP 3 THRESHOLD": "80", "PRIME-PVCAM Version": "3.7.1", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Gain": "1-HDR", "640 Shutter-TriggerSequenceLength": "1000", "528 AO-SupportsTriggering": "0", "445 AO-TriggerInputLine": "/Dev1/PFI0", "OpticalPath-Name": "OpticalPath", "PRIME-PP 4 ENABLED": "Yes", "PRIME-PVCAM Adapter Version": "1.3.29", "COM3-Name": "COM3", "405 AO-MaxVolts": "5.0000", "520 Shutter-Block voltage": "false", "PRIME-Timing-ClearingTimeNs": "49344000.0000", "Core-Focus": "PIEZO", "528 AO-Block voltage": "false", "TE2000-Description": "Nikon TE2000 hub", "528 AO-MinVolts": "0.0000", "COM4-Parity": "None", "Summary": { "Slices": 11, "ChContrastMin": [ 0, 0, 0, 0, 0, 0 ], "PixelAspect": 1, "SlicesFirst": true, "Interval_ms": 0, "Time": "2019-12-17 03:59:33 -0500", "Prefix": "seq_7", "InitialPositionList": [ { "Label": "Pos0", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [149.91], "Focus": [16657.7], "XYStage": [ 71629.7, 19152.9 ] }, "GridColumnIndex": 0 }, { "Label": "Pos1", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [190.82], "Focus": [16485.4], "XYStage": [ 65702.3, 19396.600000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos2", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [194.69], "Focus": [16446.8], "XYStage": [ 59880.5, 19007.8 ] }, "GridColumnIndex": 0 }, { "Label": "Pos3", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [145.04], "Focus": [16446.6], "XYStage": [ 53616.9, 18818.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos4", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [169.57500000000002], "Focus": [16446.5], "XYStage": [ 47662.8, 18817.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos5", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [131.805], "Focus": [16446.4], "XYStage": [ 41918.8, 18298.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos6", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [113.145], "Focus": [16446.5], "XYStage": [ 36129.9, 18895.8 ] }, "GridColumnIndex": 0 }, { "Label": "Pos7", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [141.005], "Focus": [16446.6], "XYStage": [ 30299.100000000002, 18895.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos8", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [140.97], "Focus": [16446.5], "XYStage": [ 23952.100000000002, 19156 ] }, "GridColumnIndex": 0 }, { "Label": "Pos9", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [184.57500000000002], "Focus": [16677.9], "XYStage": [ 72866.8, -14809.300000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos10", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [167.95000000000002], "Focus": [16557.9], "XYStage": [ 66515.6, -14371.300000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos11", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [168], "Focus": [16618.9], "XYStage": [ 59724.3, -13964.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos12", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [203.205], "Focus": [16779.6], "XYStage": [ 53862.3, -13494.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos13", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [203.20000000000002], "Focus": [16749], "XYStage": [ 48108.3, -13805.400000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos14", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [203.19], "Focus": [16675.9], "XYStage": [ 41645, -13804.900000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos15", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [203.21], "Focus": [16675.7], "XYStage": [ 35352.6, -13805.6 ] }, "GridColumnIndex": 0 }, { "Label": "Pos16", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [203.225], "Focus": [16675.8], "XYStage": [ 29298, -13447.400000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos17", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [203.215], "Focus": [16675.8], "XYStage": [ 23269.100000000002, -13012.300000000001 ] }, "GridColumnIndex": 0 } ], "z-step_um": 10, "GridRow": 0, "BitDepth": 16, "Depth": 2, "Channels": 6, "Source": "Micro-Manager", "ChColors": [ -16711681, -256, -65281, -1, -1, -16776961 ], "MetadataVersion": 10, "KeepShutterOpenChannels": true, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536, 65536 ], "CustomIntervals_ms": [], "Height": 1200, "MicroManagerVersion": "1.4.23 20180219", "GridColumn": 0, "UUID": "3ad8801b-5699-40d6-813b-4ad840863e38", "PixelType": "GRAY16", "Width": 1200, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\Yan\\20191216-YC113-S11S12-miseq", "Frames": 1, "Comment": "", "UserName": "Nikon", "PixelSize_um": 1.1, "ROI": [ 0, 0, 1200, 1200 ], "Date": "2019-12-17", "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC", "P-DAPI-high" ], "KeepShutterOpenSlices": true, "IJType": 1, "ComputerName": "ZadorConfocal2", "Positions": 18, "PositionIndex": 17, "CameraTimeout": 20000, "TimeFirst": false }, "640 AO-Name": "AnalogIO", "Vincent-D3-Command": "Close" }, "FrameKey-0-3-4": { "COM4-StopBits": "1", "528 AO-MaxVolts": "5.0000", "XLIGHT Excitation Wheel-State": "1", "470 Shutter-Name": "AnalogIO", "Core-Shutter": "640", "555 AO-Demo": "No", "XLIGHT Dichroic-State": "2", "XYStage-StepSize-Y": "0.1000", "ZPositionUm": 193.215, "XYStage-StepSize-X": "0.1000", "PRIME-FirmwareVersion": "20.28", "PRIME-ClearCycles": "2", "520-Name": "DA Shutter", "405 Shutter-Name": "AnalogIO", "PIEZO-StepSize": "0.0050", "PRIME-ClearMode": "Pre-Exposure", "470 AO-MinVolts": "0.0000", "405 AO-SupportsTriggering": "1", "CameraChannelIndex": 0, "XYStage-StartSpeed": "500.0000", "COM9-StopBits": "2", "405 AO-Description": "NI DAC", "COM4-DelayBetweenCharsMs": "0.0000", "445 AO-Block voltage": "false", "520 AO-TriggerInputLine": "/Dev1/PFI0", "555 AO-Description": "NI DAC", "520 AO-TriggerSampleRate": "100000", "COM4-Verbose": "1", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM4-Name": "COM4", "405 Shutter-Volts": "0.0000", "Vincent-D3-Port": "COM1", "PRIME-PP 4 MIN ADU AFFECTED": "200", "470 Shutter-MinVolts": "0.0000", "PRIME-PP 3 ENABLED": "Yes", "470 AO-Description": "NI DAC", "PRIME-Description": "PVCAM API device adapter", "520 Shutter-MaxVolts": "5.0000", "520 Shutter-Volts": "0.0000", "528 AO-Sequenceable": "1", "PRIME-Binning": "1x1", "555 Shutter-MaxVolts": "5.0000", "528 Shutter-TriggerSequenceLength": "1000", "405 Shutter-Block voltage": "false", "XLIGHT Spin motor-State": "1", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "Vincent-D3-Time to open (ms)": "35.0000", "Vincent-D3-Time to close (ms)": "35.0000", "445 AO-TriggerSampleRate": "100000", "640 Shutter-IOChannel": "Dev1/ao14", "555 AO-Sequenceable": "1", "520 AO-Name": "AnalogIO", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "470 AO-Block voltage": "false", "COM1-Verbose": "1", "COM9-AnswerTimeout": "10000.0000", "528 Shutter-Name": "AnalogIO", "PRIME-Trigger Timeout (secs)": "10", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "NextFrame": 0, "470 AO-TriggerSequenceLength": "1000", "PRIME-TransposeXY": "0", "445 AO-TriggerSequenceLength": "1000", "COM1-Name": "COM1", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "COM9-DataBits": "8", "COM1-AnswerTimeout": "500.0000", "Camera": "", "TE2000-Version": "V2.50-V2.50-V2.10", "528 Shutter-Volts": "0.0000", "XLIGHT Hub-Name": "XLIGHT Hub", "PIEZO-Description": "Ludl stage driver adapter", "PositionName": "Pos17", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSampleRate": "100000", "PRIME-FanSpeedSetpoint": "High", "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "405 AO-TriggerSampleRate": "100000", "470 Shutter-Block voltage": "false", "470-State": "0", "COM3-Parity": "None", "405 AO-Volts": "0.0000", "PRIME-TransposeMirrorY": "0", "PRIME-ChipName": "GS144BSI", "470 AO-Volts": "0.0000", "PRIME-TransposeMirrorX": "0", "445 Shutter-SupportsTriggering": "0", "470 Shutter-SupportsTriggering": "0", "XLIGHT Hub-Description": "XLIGHT hub", "PRIME-PreampOffLimit": "10000", "470 AO-Sequenceable": "1", "528 Shutter-MaxVolts": "5.0000", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "Core-XYStage": "XYStage", "470 AO-TriggerSampleRate": "100000", "PRIME-Timing-PreTriggerDelayNs": "24672000.0000", "528-DA Device": "528 Shutter", "Focus-Description": "Nikon TE2000 focus adapter", "FilterBlock-State": "5", "520 AO-Demo": "No", "COM3-DelayBetweenCharsMs": "0.0000", "470 Shutter-Volts": "0.0000", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "PRIME-PP 2 THRESHOLD": "125", "PRIME-PP 4 THRESHOLD": "75", "XYStage-Acceleration": "4.0000", "470 Shutter-Demo": "No", "PRIME-Name": "PMPCIECam00", "640-DA Device": "640 Shutter", "405 AO-Block voltage": "false", "ElapsedTime-ms": 738441, "PRIME-Offset": "100", "445 AO-IOChannel": "Dev1/ao1", "640 AO-MaxVolts": "5.0000", "PositionIndex": 17, "PRIME-PP 1 ENABLED": "Yes", "COM1-BaudRate": "9600", "640-State": "1", "COM3-Verbose": "1", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "LudlController-TransmissionDelay": "0", "PRIME-FullWellCapacity": "62000", "PRIME-SMARTStreamingEnabled": "No", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "640 Shutter-Description": "NI DAC", "528-Description": "DA device that is used as a shutter", "COM4-Description": "Serial port driver (boost:asio)", "405 Shutter-Description": "NI DAC", "TE2000-Name": "TE2000", "528 Shutter-Description": "NI DAC", "COM9-Description": "Serial port driver (boost:asio)", "LudlController-Name": "LudlController", "445 Shutter-TriggerSequenceLength": "1000", "PRIME-SerialNumber": "A18B203008", "445 AO-Volts": "0.0000", "PRIME-X-dimension": "1200", "PRIME-ShutterCloseDelay": "0", "XLIGHT Disk slider-State": "2", "Position": "Default", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 AO-IOChannel": "Dev1/ao5", "520 AO-IOChannel": "Dev1/ao4", "528 AO-TriggerSequenceLength": "1000", "405 Shutter-MaxVolts": "5.0000", "470-DA Device": "470 Shutter", "PRIME-FTCapable": "No", "Core-ChannelGroup": "Channels", "555 AO-SupportsTriggering": "0", "PRIME-Y-dimension": "1200", "Frame": 0, "555 Shutter-Sequenceable": "1", "PRIME-Timing-ExposureTimeNs": "499998000.0000", "Core-ImageProcessor": "", "528-State": "0", "520 AO-Block voltage": "false", "XLIGHT Excitation Wheel-Label": "QUAD", "640 Shutter-Demo": "No", "COM3-StopBits": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "520 Shutter-IOChannel": "Dev1/ao11", "Width": 1200, "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-SupportsTriggering": "0", "640 AO-TriggerSampleRate": "100000", "520-Description": "DA device that is used as a shutter", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "405 Shutter-Sequenceable": "1", "405 Shutter-SupportsTriggering": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-TriggerSampleRate": "100000", "640 Shutter-MaxVolts": "5.0000", "555 AO-MaxVolts": "5.0000", "520 AO-TriggerSequenceLength": "1000", "COM3-BaudRate": "9600", "COM9-BaudRate": "9600", "Vincent-D3-Channel #": "Ch. #1", "PRIME-PMode": "Normal", "OpticalPath-Label": "Left(100)", "BitDepth": 16, "555 AO-TriggerSequenceLength": "1000", "445 Shutter-Description": "NI DAC", "405 AO-Sequenceable": "1", "PRIME-AcquisitionMethod": "Callbacks", "555 AO-Name": "AnalogIO", "PRIME-ShutterMode": "Pre-Exposure", "COM3-Handshaking": "Off", "445 AO-SupportsTriggering": "0", "ROI": "0-0-1200-1200", "COM9-Verbose": "1", "PRIME-Color": "OFF", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "PRIME-ShutterOpenDelay": "0", "COM1-Parity": "None", "Slice": 4, "SlicePosition": -10, "COM9-DelayBetweenCharsMs": "0.0000", "555-Name": "DA Shutter", "PIEZO-LudlSingleAxisName": "B", "XLIGHT Emission Wheel-Label": "725/40 (C)", "640 Shutter-Name": "AnalogIO", "640 AO-Sequenceable": "1", "520 Shutter-Demo": "No", "528 Shutter-Demo": "No", "470-Name": "DA Shutter", "520-DA Device": "520 Shutter", "XYStage-Description": "Ludl XY stage driver adapter", "445 Shutter-MinVolts": "0.0000", "528 AO-TriggerSampleRate": "100000", "405 AO-MinVolts": "0.0000", "405 Shutter-TriggerSequenceLength": "1000", "Height": 1200, "555 AO-Block voltage": "false", "640 Shutter-Volts": "5.0000", "PixelType": "GRAY16", "445 Shutter-Block voltage": "false", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "520 Shutter-TriggerSequenceLength": "1000", "COM9-Name": "COM9", "Core-SLM": "", "555 AO-Volts": "0.0000", "555 Shutter-Block voltage": "false", "XYStage-Speed": "1800.0000", "520 AO-MaxVolts": "5.0000", "528 Shutter-SupportsTriggering": "0", "COM1-Description": "Serial port driver (boost:asio)", "PRIME-MetadataEnabled": "No", "520 Shutter-MinVolts": "0.0000", "470 Shutter-Sequenceable": "1", "PRIME-PreampDelay": "0", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-MinVolts": "0.0000", "ChannelIndex": 3, "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Core-AutoShutter": "1", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "Exposure-ms": 520, "PRIME-PP 2 ENABLED": "Yes", "640 Shutter-TriggerSampleRate": "100000", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "470 Shutter-IOChannel": "Dev1/ao10", "405 AO-Demo": "No", "PixelSizeUm": 1.1, "470 AO-Demo": "No", "470 Shutter-MaxVolts": "5.0000", "Vincent-D3-Name": "Vincent-D3", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FileName": "img_000000000_Il-C_004.tif", "LudlController-Reset": "Operate", "PRIME-Exposure": "520.00", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "445-Name": "DA Shutter", "555 Shutter-Name": "AnalogIO", "520 Shutter-TriggerSampleRate": "100000", "445 Shutter-IOChannel": "Dev1/ao9", "PRIME-TransposeCorrection": "0", "XYStage-StepSize": "0.1000", "445-Description": "DA device that is used as a shutter", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 AO-IOChannel": "Dev1/ao7", "520 Shutter-Name": "AnalogIO", "COM3-DataBits": "8", "640 AO-Volts": "4.5000", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "555 Shutter-IOChannel": "Dev1/ao13", "WaitInterval": null, "520 Shutter-Sequenceable": "1", "YPositionUm": -13012.300000000001, "445-State": "0", "445-DA Device": "445 Shutter", "PRIME-CircularBufferAutoSize": "ON", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "555 AO-TriggerSampleRate": "100000", "445 Shutter-Volts": "0.0000", "XLIGHT Hub-Port": "COM3", "Core-AutoFocus": "", "COM4-Handshaking": "Off", "528 Shutter-MinVolts": "0.0000", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "445 Shutter-Name": "AnalogIO", "528 Shutter-Sequenceable": "1", "XYStage-TransposeMirrorX": "0", "XYStage-TransposeMirrorY": "0", "PRIME-ReadoutRate": "100MHz 16bit", "XYStage-Name": "Stage", "640 AO-SupportsTriggering": "0", "640-Name": "DA Shutter", "640 Shutter-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "640 AO-Block voltage": "false", "445 Shutter-Sequenceable": "1", "470 AO-MaxVolts": "5.0000", "COM4-BaudRate": "9600", "470-Description": "DA device that is used as a shutter", "520 Shutter-Description": "NI DAC", "PRIME-CCDTemperature": "-14.9200", "Time": "2019-12-17 03:59:55 -0500", "640 AO-TriggerSequenceLength": "1000", "470 AO-Name": "AnalogIO", "XLIGHT Disk slider-Label": "Disk Pos 40um", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "Binning": "1x1", "TE2000-ModelType": "1", "COM3-AnswerTimeout": "500.0000", "555 Shutter-SupportsTriggering": "0", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "TE2000-Port": "COM4", "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Description": "NI DAC", "COM4-AnswerTimeout": "500.0000", "COM1-DelayBetweenCharsMs": "0.0000", "FilterBlock-Label": "Confocal", "445 AO-Name": "AnalogIO", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-Timing-ReadoutTimeNs": "24672000.0000", "528 AO-Demo": "No", "520 AO-Sequenceable": "1", "555-Description": "DA device that is used as a shutter", "405-DA Device": "405 Shutter", "405 AO-TriggerSequenceLength": "1000", "640 AO-MinVolts": "0.0000", "640 Shutter-Block voltage": "false", "FrameIndex": 0, "PRIME-Port": "CMOS", "555 AO-MinVolts": "0.0000", "COM3-Description": "Serial port driver (boost:asio)", "528 AO-Description": "NI DAC", "555 AO-IOChannel": "Dev1/ao6", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "555 AO-TriggerInputLine": "/Dev1/PFI0", "PRIME-ActualInterval-ms": "84.6200", "LudlController-Port": "COM9", "445 Shutter-Demo": "No", "PRIME-Trigger-Expose Out-Mux": "1", "SliceIndex": 4, "PRIME-CCDTemperatureSetPoint": "-15.0000", "405 Shutter-MinVolts": "0.0000", "PRIME-ExposeOutMode": "First Row", "AxisPositions": null, "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Volts": "0.0000", "528-Name": "DA Shutter", "555-State": "0", "XLIGHT Dichroic-Label": "Di03-R635-t1", "445 AO-Sequenceable": "1", "405-Name": "DA Shutter", "405 AO-IOChannel": "Dev1/ao0", "Core-Camera": "PRIME", "Focus-Name": "Focus", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "405 Shutter-IOChannel": "Dev1/ao8", "640 AO-Demo": "No", "640 Shutter-SupportsTriggering": "0", "445 AO-Demo": "No", "555-DA Device": "555 Shutter", "470 Shutter-Description": "NI DAC", "528 AO-Volts": "0.0000", "COM1-DataBits": "8", "Vincent-D3-Address": "x", "520 AO-SupportsTriggering": "0", "405-Description": "DA device that is used as a shutter", "640 Shutter-MinVolts": "0.0000", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "405 AO-Name": "AnalogIO", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "640 AO-TriggerInputLine": "/Dev1/PFI0", "405 AO-TriggerInputLine": "/Dev1/PFI0", "640-Description": "DA device that is used as a shutter", "445 Shutter-TriggerSampleRate": "100000", "470 Shutter-TriggerSequenceLength": "1000", "COM1-StopBits": "1", "520 Shutter-SupportsTriggering": "0", "405-State": "0", "Core-Initialize": "1", "XLIGHT Emission Wheel-State": "3", "520 AO-Description": "NI DAC", "528 AO-TriggerInputLine": "/Dev1/PFI0", "445 Shutter-MaxVolts": "5.0000", "COM9-Handshaking": "Off", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "XLIGHT Spin motor-Label": "On (spinning)", "PRIME-PP 5 ENABLED": "No", "UUID": "678570df-643c-4068-afea-b85e2bddd434", "PRIME-TriggerMode": "Internal Trigger", "COM1-Handshaking": "Off", "PRIME-Timing-PostTriggerDelayNs": "49344000.0000", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "Focus-Position": "16732.9000", "528 Shutter-Block voltage": "false", "Channel": "Il-C", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "555 Shutter-Demo": "No", "405 Shutter-Demo": "No", "528 AO-Name": "AnalogIO", "520-State": "0", "Core-Galvo": "", "405 Shutter-TriggerSampleRate": "100000", "Focus-FocusStepSize_(nm)": "100", "PRIME-PixelType": "16bit", "COM4-DataBits": "8", "470 AO-IOChannel": "Dev1/ao2", "520 AO-MinVolts": "0.0000", "COM9-Parity": "None", "PRIME-CircularBufferEnabled": "ON", "PRIME-PP 6 Reset": "No", "640 AO-Description": "NI DAC", "Source": "PRIME", "445 AO-MinVolts": "0.0000", "PIEZO-Name": "Stage", "445 AO-Description": "NI DAC", "PRIME-CircularBufferFrameCount": "46", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "520 AO-Volts": "0.0000", "528 Shutter-TriggerSampleRate": "100000", "XPositionUm": 23269.100000000002, "OpticalPath-State": "4", "PRIME-PP 3 THRESHOLD": "80", "PRIME-PVCAM Version": "3.7.1", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Gain": "1-HDR", "640 Shutter-TriggerSequenceLength": "1000", "528 AO-SupportsTriggering": "0", "445 AO-TriggerInputLine": "/Dev1/PFI0", "OpticalPath-Name": "OpticalPath", "PRIME-PP 4 ENABLED": "Yes", "PRIME-PVCAM Adapter Version": "1.3.29", "COM3-Name": "COM3", "405 AO-MaxVolts": "5.0000", "520 Shutter-Block voltage": "false", "PRIME-Timing-ClearingTimeNs": "49344000.0000", "Core-Focus": "PIEZO", "528 AO-Block voltage": "false", "TE2000-Description": "Nikon TE2000 hub", "528 AO-MinVolts": "0.0000", "COM4-Parity": "None", "Summary": { "Slices": 11, "ChContrastMin": [ 0, 0, 0, 0, 0, 0 ], "PixelAspect": 1, "SlicesFirst": true, "Interval_ms": 0, "Time": "2019-12-17 03:59:33 -0500", "Prefix": "seq_7", "InitialPositionList": [ { "Label": "Pos0", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [149.91], "Focus": [16657.7], "XYStage": [ 71629.7, 19152.9 ] }, "GridColumnIndex": 0 }, { "Label": "Pos1", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [190.82], "Focus": [16485.4], "XYStage": [ 65702.3, 19396.600000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos2", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [194.69], "Focus": [16446.8], "XYStage": [ 59880.5, 19007.8 ] }, "GridColumnIndex": 0 }, { "Label": "Pos3", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [145.04], "Focus": [16446.6], "XYStage": [ 53616.9, 18818.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos4", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [169.57500000000002], "Focus": [16446.5], "XYStage": [ 47662.8, 18817.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos5", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [131.805], "Focus": [16446.4], "XYStage": [ 41918.8, 18298.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos6", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [113.145], "Focus": [16446.5], "XYStage": [ 36129.9, 18895.8 ] }, "GridColumnIndex": 0 }, { "Label": "Pos7", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [141.005], "Focus": [16446.6], "XYStage": [ 30299.100000000002, 18895.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos8", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [140.97], "Focus": [16446.5], "XYStage": [ 23952.100000000002, 19156 ] }, "GridColumnIndex": 0 }, { "Label": "Pos9", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [184.57500000000002], "Focus": [16677.9], "XYStage": [ 72866.8, -14809.300000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos10", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [167.95000000000002], "Focus": [16557.9], "XYStage": [ 66515.6, -14371.300000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos11", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [168], "Focus": [16618.9], "XYStage": [ 59724.3, -13964.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos12", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [203.205], "Focus": [16779.6], "XYStage": [ 53862.3, -13494.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos13", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [203.20000000000002], "Focus": [16749], "XYStage": [ 48108.3, -13805.400000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos14", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [203.19], "Focus": [16675.9], "XYStage": [ 41645, -13804.900000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos15", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [203.21], "Focus": [16675.7], "XYStage": [ 35352.6, -13805.6 ] }, "GridColumnIndex": 0 }, { "Label": "Pos16", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [203.225], "Focus": [16675.8], "XYStage": [ 29298, -13447.400000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos17", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [203.215], "Focus": [16675.8], "XYStage": [ 23269.100000000002, -13012.300000000001 ] }, "GridColumnIndex": 0 } ], "z-step_um": 10, "GridRow": 0, "BitDepth": 16, "Depth": 2, "Channels": 6, "Source": "Micro-Manager", "ChColors": [ -16711681, -256, -65281, -1, -1, -16776961 ], "MetadataVersion": 10, "KeepShutterOpenChannels": true, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536, 65536 ], "CustomIntervals_ms": [], "Height": 1200, "MicroManagerVersion": "1.4.23 20180219", "GridColumn": 0, "UUID": "3ad8801b-5699-40d6-813b-4ad840863e38", "PixelType": "GRAY16", "Width": 1200, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\Yan\\20191216-YC113-S11S12-miseq", "Frames": 1, "Comment": "", "UserName": "Nikon", "PixelSize_um": 1.1, "ROI": [ 0, 0, 1200, 1200 ], "Date": "2019-12-17", "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC", "P-DAPI-high" ], "KeepShutterOpenSlices": true, "IJType": 1, "ComputerName": "ZadorConfocal2", "Positions": 18, "PositionIndex": 17, "CameraTimeout": 20000, "TimeFirst": false }, "640 AO-Name": "AnalogIO", "Vincent-D3-Command": "Close" }, "FrameKey-0-3-5": { "COM4-StopBits": "1", "528 AO-MaxVolts": "5.0000", "XLIGHT Excitation Wheel-State": "1", "470 Shutter-Name": "AnalogIO", "Core-Shutter": "640", "555 AO-Demo": "No", "XLIGHT Dichroic-State": "2", "XYStage-StepSize-Y": "0.1000", "ZPositionUm": 203.215, "XYStage-StepSize-X": "0.1000", "PRIME-FirmwareVersion": "20.28", "PRIME-ClearCycles": "2", "520-Name": "DA Shutter", "405 Shutter-Name": "AnalogIO", "PIEZO-StepSize": "0.0050", "PRIME-ClearMode": "Pre-Exposure", "470 AO-MinVolts": "0.0000", "405 AO-SupportsTriggering": "1", "CameraChannelIndex": 0, "XYStage-StartSpeed": "500.0000", "COM9-StopBits": "2", "405 AO-Description": "NI DAC", "COM4-DelayBetweenCharsMs": "0.0000", "445 AO-Block voltage": "false", "520 AO-TriggerInputLine": "/Dev1/PFI0", "555 AO-Description": "NI DAC", "520 AO-TriggerSampleRate": "100000", "COM4-Verbose": "1", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM4-Name": "COM4", "405 Shutter-Volts": "0.0000", "Vincent-D3-Port": "COM1", "PRIME-PP 4 MIN ADU AFFECTED": "200", "470 Shutter-MinVolts": "0.0000", "PRIME-PP 3 ENABLED": "Yes", "470 AO-Description": "NI DAC", "PRIME-Description": "PVCAM API device adapter", "520 Shutter-MaxVolts": "5.0000", "520 Shutter-Volts": "0.0000", "528 AO-Sequenceable": "1", "PRIME-Binning": "1x1", "555 Shutter-MaxVolts": "5.0000", "528 Shutter-TriggerSequenceLength": "1000", "405 Shutter-Block voltage": "false", "XLIGHT Spin motor-State": "1", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "Vincent-D3-Time to open (ms)": "35.0000", "Vincent-D3-Time to close (ms)": "35.0000", "445 AO-TriggerSampleRate": "100000", "640 Shutter-IOChannel": "Dev1/ao14", "555 AO-Sequenceable": "1", "520 AO-Name": "AnalogIO", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "470 AO-Block voltage": "false", "COM1-Verbose": "1", "COM9-AnswerTimeout": "10000.0000", "528 Shutter-Name": "AnalogIO", "PRIME-Trigger Timeout (secs)": "10", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "NextFrame": 0, "470 AO-TriggerSequenceLength": "1000", "PRIME-TransposeXY": "0", "445 AO-TriggerSequenceLength": "1000", "COM1-Name": "COM1", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "COM9-DataBits": "8", "COM1-AnswerTimeout": "500.0000", "Camera": "", "TE2000-Version": "V2.50-V2.50-V2.10", "528 Shutter-Volts": "0.0000", "XLIGHT Hub-Name": "XLIGHT Hub", "PIEZO-Description": "Ludl stage driver adapter", "PositionName": "Pos17", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSampleRate": "100000", "PRIME-FanSpeedSetpoint": "High", "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "405 AO-TriggerSampleRate": "100000", "470 Shutter-Block voltage": "false", "470-State": "0", "COM3-Parity": "None", "405 AO-Volts": "0.0000", "PRIME-TransposeMirrorY": "0", "PRIME-ChipName": "GS144BSI", "470 AO-Volts": "0.0000", "PRIME-TransposeMirrorX": "0", "445 Shutter-SupportsTriggering": "0", "470 Shutter-SupportsTriggering": "0", "XLIGHT Hub-Description": "XLIGHT hub", "PRIME-PreampOffLimit": "10000", "470 AO-Sequenceable": "1", "528 Shutter-MaxVolts": "5.0000", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "Core-XYStage": "XYStage", "470 AO-TriggerSampleRate": "100000", "PRIME-Timing-PreTriggerDelayNs": "24672000.0000", "528-DA Device": "528 Shutter", "Focus-Description": "Nikon TE2000 focus adapter", "FilterBlock-State": "5", "520 AO-Demo": "No", "COM3-DelayBetweenCharsMs": "0.0000", "470 Shutter-Volts": "0.0000", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "PRIME-PP 2 THRESHOLD": "125", "PRIME-PP 4 THRESHOLD": "75", "XYStage-Acceleration": "4.0000", "470 Shutter-Demo": "No", "PRIME-Name": "PMPCIECam00", "640-DA Device": "640 Shutter", "405 AO-Block voltage": "false", "ElapsedTime-ms": 739210, "PRIME-Offset": "100", "445 AO-IOChannel": "Dev1/ao1", "640 AO-MaxVolts": "5.0000", "PositionIndex": 17, "PRIME-PP 1 ENABLED": "Yes", "COM1-BaudRate": "9600", "640-State": "1", "COM3-Verbose": "1", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "LudlController-TransmissionDelay": "0", "PRIME-FullWellCapacity": "62000", "PRIME-SMARTStreamingEnabled": "No", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "640 Shutter-Description": "NI DAC", "528-Description": "DA device that is used as a shutter", "COM4-Description": "Serial port driver (boost:asio)", "405 Shutter-Description": "NI DAC", "TE2000-Name": "TE2000", "528 Shutter-Description": "NI DAC", "COM9-Description": "Serial port driver (boost:asio)", "LudlController-Name": "LudlController", "445 Shutter-TriggerSequenceLength": "1000", "PRIME-SerialNumber": "A18B203008", "445 AO-Volts": "0.0000", "PRIME-X-dimension": "1200", "PRIME-ShutterCloseDelay": "0", "XLIGHT Disk slider-State": "2", "Position": "Default", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 AO-IOChannel": "Dev1/ao5", "520 AO-IOChannel": "Dev1/ao4", "528 AO-TriggerSequenceLength": "1000", "405 Shutter-MaxVolts": "5.0000", "470-DA Device": "470 Shutter", "PRIME-FTCapable": "No", "Core-ChannelGroup": "Channels", "555 AO-SupportsTriggering": "0", "PRIME-Y-dimension": "1200", "Frame": 0, "555 Shutter-Sequenceable": "1", "PRIME-Timing-ExposureTimeNs": "499998000.0000", "Core-ImageProcessor": "", "528-State": "0", "520 AO-Block voltage": "false", "XLIGHT Excitation Wheel-Label": "QUAD", "640 Shutter-Demo": "No", "COM3-StopBits": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "520 Shutter-IOChannel": "Dev1/ao11", "Width": 1200, "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-SupportsTriggering": "0", "640 AO-TriggerSampleRate": "100000", "520-Description": "DA device that is used as a shutter", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "405 Shutter-Sequenceable": "1", "405 Shutter-SupportsTriggering": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-TriggerSampleRate": "100000", "640 Shutter-MaxVolts": "5.0000", "555 AO-MaxVolts": "5.0000", "520 AO-TriggerSequenceLength": "1000", "COM3-BaudRate": "9600", "COM9-BaudRate": "9600", "Vincent-D3-Channel #": "Ch. #1", "PRIME-PMode": "Normal", "OpticalPath-Label": "Left(100)", "BitDepth": 16, "555 AO-TriggerSequenceLength": "1000", "445 Shutter-Description": "NI DAC", "405 AO-Sequenceable": "1", "PRIME-AcquisitionMethod": "Callbacks", "555 AO-Name": "AnalogIO", "PRIME-ShutterMode": "Pre-Exposure", "COM3-Handshaking": "Off", "445 AO-SupportsTriggering": "0", "ROI": "0-0-1200-1200", "COM9-Verbose": "1", "PRIME-Color": "OFF", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "PRIME-ShutterOpenDelay": "0", "COM1-Parity": "None", "Slice": 5, "SlicePosition": 0, "COM9-DelayBetweenCharsMs": "0.0000", "555-Name": "DA Shutter", "PIEZO-LudlSingleAxisName": "B", "XLIGHT Emission Wheel-Label": "725/40 (C)", "640 Shutter-Name": "AnalogIO", "640 AO-Sequenceable": "1", "520 Shutter-Demo": "No", "528 Shutter-Demo": "No", "470-Name": "DA Shutter", "520-DA Device": "520 Shutter", "XYStage-Description": "Ludl XY stage driver adapter", "445 Shutter-MinVolts": "0.0000", "528 AO-TriggerSampleRate": "100000", "405 AO-MinVolts": "0.0000", "405 Shutter-TriggerSequenceLength": "1000", "Height": 1200, "555 AO-Block voltage": "false", "640 Shutter-Volts": "5.0000", "PixelType": "GRAY16", "445 Shutter-Block voltage": "false", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "520 Shutter-TriggerSequenceLength": "1000", "COM9-Name": "COM9", "Core-SLM": "", "555 AO-Volts": "0.0000", "555 Shutter-Block voltage": "false", "XYStage-Speed": "1800.0000", "520 AO-MaxVolts": "5.0000", "528 Shutter-SupportsTriggering": "0", "COM1-Description": "Serial port driver (boost:asio)", "PRIME-MetadataEnabled": "No", "520 Shutter-MinVolts": "0.0000", "470 Shutter-Sequenceable": "1", "PRIME-PreampDelay": "0", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-MinVolts": "0.0000", "ChannelIndex": 3, "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Core-AutoShutter": "1", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "Exposure-ms": 520, "PRIME-PP 2 ENABLED": "Yes", "640 Shutter-TriggerSampleRate": "100000", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "470 Shutter-IOChannel": "Dev1/ao10", "405 AO-Demo": "No", "PixelSizeUm": 1.1, "470 AO-Demo": "No", "470 Shutter-MaxVolts": "5.0000", "Vincent-D3-Name": "Vincent-D3", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FileName": "img_000000000_Il-C_005.tif", "LudlController-Reset": "Operate", "PRIME-Exposure": "520.00", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "445-Name": "DA Shutter", "555 Shutter-Name": "AnalogIO", "520 Shutter-TriggerSampleRate": "100000", "445 Shutter-IOChannel": "Dev1/ao9", "PRIME-TransposeCorrection": "0", "XYStage-StepSize": "0.1000", "445-Description": "DA device that is used as a shutter", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 AO-IOChannel": "Dev1/ao7", "520 Shutter-Name": "AnalogIO", "COM3-DataBits": "8", "640 AO-Volts": "4.5000", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "555 Shutter-IOChannel": "Dev1/ao13", "WaitInterval": null, "520 Shutter-Sequenceable": "1", "YPositionUm": -13012.300000000001, "445-State": "0", "445-DA Device": "445 Shutter", "PRIME-CircularBufferAutoSize": "ON", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "555 AO-TriggerSampleRate": "100000", "445 Shutter-Volts": "0.0000", "XLIGHT Hub-Port": "COM3", "Core-AutoFocus": "", "COM4-Handshaking": "Off", "528 Shutter-MinVolts": "0.0000", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "445 Shutter-Name": "AnalogIO", "528 Shutter-Sequenceable": "1", "XYStage-TransposeMirrorX": "0", "XYStage-TransposeMirrorY": "0", "PRIME-ReadoutRate": "100MHz 16bit", "XYStage-Name": "Stage", "640 AO-SupportsTriggering": "0", "640-Name": "DA Shutter", "640 Shutter-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "640 AO-Block voltage": "false", "445 Shutter-Sequenceable": "1", "470 AO-MaxVolts": "5.0000", "COM4-BaudRate": "9600", "470-Description": "DA device that is used as a shutter", "520 Shutter-Description": "NI DAC", "PRIME-CCDTemperature": "-14.9200", "Time": "2019-12-17 03:59:56 -0500", "640 AO-TriggerSequenceLength": "1000", "470 AO-Name": "AnalogIO", "XLIGHT Disk slider-Label": "Disk Pos 40um", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "Binning": "1x1", "TE2000-ModelType": "1", "COM3-AnswerTimeout": "500.0000", "555 Shutter-SupportsTriggering": "0", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "TE2000-Port": "COM4", "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Description": "NI DAC", "COM4-AnswerTimeout": "500.0000", "COM1-DelayBetweenCharsMs": "0.0000", "FilterBlock-Label": "Confocal", "445 AO-Name": "AnalogIO", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-Timing-ReadoutTimeNs": "24672000.0000", "528 AO-Demo": "No", "520 AO-Sequenceable": "1", "555-Description": "DA device that is used as a shutter", "405-DA Device": "405 Shutter", "405 AO-TriggerSequenceLength": "1000", "640 AO-MinVolts": "0.0000", "640 Shutter-Block voltage": "false", "FrameIndex": 0, "PRIME-Port": "CMOS", "555 AO-MinVolts": "0.0000", "COM3-Description": "Serial port driver (boost:asio)", "528 AO-Description": "NI DAC", "555 AO-IOChannel": "Dev1/ao6", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "555 AO-TriggerInputLine": "/Dev1/PFI0", "PRIME-ActualInterval-ms": "84.6200", "LudlController-Port": "COM9", "445 Shutter-Demo": "No", "PRIME-Trigger-Expose Out-Mux": "1", "SliceIndex": 5, "PRIME-CCDTemperatureSetPoint": "-15.0000", "405 Shutter-MinVolts": "0.0000", "PRIME-ExposeOutMode": "First Row", "AxisPositions": null, "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Volts": "0.0000", "528-Name": "DA Shutter", "555-State": "0", "XLIGHT Dichroic-Label": "Di03-R635-t1", "445 AO-Sequenceable": "1", "405-Name": "DA Shutter", "405 AO-IOChannel": "Dev1/ao0", "Core-Camera": "PRIME", "Focus-Name": "Focus", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "405 Shutter-IOChannel": "Dev1/ao8", "640 AO-Demo": "No", "640 Shutter-SupportsTriggering": "0", "445 AO-Demo": "No", "555-DA Device": "555 Shutter", "470 Shutter-Description": "NI DAC", "528 AO-Volts": "0.0000", "COM1-DataBits": "8", "Vincent-D3-Address": "x", "520 AO-SupportsTriggering": "0", "405-Description": "DA device that is used as a shutter", "640 Shutter-MinVolts": "0.0000", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "405 AO-Name": "AnalogIO", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "640 AO-TriggerInputLine": "/Dev1/PFI0", "405 AO-TriggerInputLine": "/Dev1/PFI0", "640-Description": "DA device that is used as a shutter", "445 Shutter-TriggerSampleRate": "100000", "470 Shutter-TriggerSequenceLength": "1000", "COM1-StopBits": "1", "520 Shutter-SupportsTriggering": "0", "405-State": "0", "Core-Initialize": "1", "XLIGHT Emission Wheel-State": "3", "520 AO-Description": "NI DAC", "528 AO-TriggerInputLine": "/Dev1/PFI0", "445 Shutter-MaxVolts": "5.0000", "COM9-Handshaking": "Off", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "XLIGHT Spin motor-Label": "On (spinning)", "PRIME-PP 5 ENABLED": "No", "UUID": "9ec29f24-1e7e-4051-8c35-236cb8150597", "PRIME-TriggerMode": "Internal Trigger", "COM1-Handshaking": "Off", "PRIME-Timing-PostTriggerDelayNs": "49344000.0000", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "Focus-Position": "16732.9000", "528 Shutter-Block voltage": "false", "Channel": "Il-C", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "555 Shutter-Demo": "No", "405 Shutter-Demo": "No", "528 AO-Name": "AnalogIO", "520-State": "0", "Core-Galvo": "", "405 Shutter-TriggerSampleRate": "100000", "Focus-FocusStepSize_(nm)": "100", "PRIME-PixelType": "16bit", "COM4-DataBits": "8", "470 AO-IOChannel": "Dev1/ao2", "520 AO-MinVolts": "0.0000", "COM9-Parity": "None", "PRIME-CircularBufferEnabled": "ON", "PRIME-PP 6 Reset": "No", "640 AO-Description": "NI DAC", "Source": "PRIME", "445 AO-MinVolts": "0.0000", "PIEZO-Name": "Stage", "445 AO-Description": "NI DAC", "PRIME-CircularBufferFrameCount": "46", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "520 AO-Volts": "0.0000", "528 Shutter-TriggerSampleRate": "100000", "XPositionUm": 23269.100000000002, "OpticalPath-State": "4", "PRIME-PP 3 THRESHOLD": "80", "PRIME-PVCAM Version": "3.7.1", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Gain": "1-HDR", "640 Shutter-TriggerSequenceLength": "1000", "528 AO-SupportsTriggering": "0", "445 AO-TriggerInputLine": "/Dev1/PFI0", "OpticalPath-Name": "OpticalPath", "PRIME-PP 4 ENABLED": "Yes", "PRIME-PVCAM Adapter Version": "1.3.29", "COM3-Name": "COM3", "405 AO-MaxVolts": "5.0000", "520 Shutter-Block voltage": "false", "PRIME-Timing-ClearingTimeNs": "49344000.0000", "Core-Focus": "PIEZO", "528 AO-Block voltage": "false", "TE2000-Description": "Nikon TE2000 hub", "528 AO-MinVolts": "0.0000", "COM4-Parity": "None", "Summary": { "Slices": 11, "ChContrastMin": [ 0, 0, 0, 0, 0, 0 ], "PixelAspect": 1, "SlicesFirst": true, "Interval_ms": 0, "Time": "2019-12-17 03:59:33 -0500", "Prefix": "seq_7", "InitialPositionList": [ { "Label": "Pos0", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [149.91], "Focus": [16657.7], "XYStage": [ 71629.7, 19152.9 ] }, "GridColumnIndex": 0 }, { "Label": "Pos1", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [190.82], "Focus": [16485.4], "XYStage": [ 65702.3, 19396.600000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos2", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [194.69], "Focus": [16446.8], "XYStage": [ 59880.5, 19007.8 ] }, "GridColumnIndex": 0 }, { "Label": "Pos3", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [145.04], "Focus": [16446.6], "XYStage": [ 53616.9, 18818.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos4", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [169.57500000000002], "Focus": [16446.5], "XYStage": [ 47662.8, 18817.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos5", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [131.805], "Focus": [16446.4], "XYStage": [ 41918.8, 18298.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos6", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [113.145], "Focus": [16446.5], "XYStage": [ 36129.9, 18895.8 ] }, "GridColumnIndex": 0 }, { "Label": "Pos7", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [141.005], "Focus": [16446.6], "XYStage": [ 30299.100000000002, 18895.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos8", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [140.97], "Focus": [16446.5], "XYStage": [ 23952.100000000002, 19156 ] }, "GridColumnIndex": 0 }, { "Label": "Pos9", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [184.57500000000002], "Focus": [16677.9], "XYStage": [ 72866.8, -14809.300000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos10", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [167.95000000000002], "Focus": [16557.9], "XYStage": [ 66515.6, -14371.300000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos11", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [168], "Focus": [16618.9], "XYStage": [ 59724.3, -13964.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos12", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [203.205], "Focus": [16779.6], "XYStage": [ 53862.3, -13494.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos13", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [203.20000000000002], "Focus": [16749], "XYStage": [ 48108.3, -13805.400000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos14", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [203.19], "Focus": [16675.9], "XYStage": [ 41645, -13804.900000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos15", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [203.21], "Focus": [16675.7], "XYStage": [ 35352.6, -13805.6 ] }, "GridColumnIndex": 0 }, { "Label": "Pos16", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [203.225], "Focus": [16675.8], "XYStage": [ 29298, -13447.400000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos17", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [203.215], "Focus": [16675.8], "XYStage": [ 23269.100000000002, -13012.300000000001 ] }, "GridColumnIndex": 0 } ], "z-step_um": 10, "GridRow": 0, "BitDepth": 16, "Depth": 2, "Channels": 6, "Source": "Micro-Manager", "ChColors": [ -16711681, -256, -65281, -1, -1, -16776961 ], "MetadataVersion": 10, "KeepShutterOpenChannels": true, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536, 65536 ], "CustomIntervals_ms": [], "Height": 1200, "MicroManagerVersion": "1.4.23 20180219", "GridColumn": 0, "UUID": "3ad8801b-5699-40d6-813b-4ad840863e38", "PixelType": "GRAY16", "Width": 1200, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\Yan\\20191216-YC113-S11S12-miseq", "Frames": 1, "Comment": "", "UserName": "Nikon", "PixelSize_um": 1.1, "ROI": [ 0, 0, 1200, 1200 ], "Date": "2019-12-17", "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC", "P-DAPI-high" ], "KeepShutterOpenSlices": true, "IJType": 1, "ComputerName": "ZadorConfocal2", "Positions": 18, "PositionIndex": 17, "CameraTimeout": 20000, "TimeFirst": false }, "640 AO-Name": "AnalogIO", "Vincent-D3-Command": "Close" }, "FrameKey-0-3-6": { "COM4-StopBits": "1", "528 AO-MaxVolts": "5.0000", "XLIGHT Excitation Wheel-State": "1", "470 Shutter-Name": "AnalogIO", "Core-Shutter": "640", "555 AO-Demo": "No", "XLIGHT Dichroic-State": "2", "XYStage-StepSize-Y": "0.1000", "ZPositionUm": 213.215, "XYStage-StepSize-X": "0.1000", "PRIME-FirmwareVersion": "20.28", "PRIME-ClearCycles": "2", "520-Name": "DA Shutter", "405 Shutter-Name": "AnalogIO", "PIEZO-StepSize": "0.0050", "PRIME-ClearMode": "Pre-Exposure", "470 AO-MinVolts": "0.0000", "405 AO-SupportsTriggering": "1", "CameraChannelIndex": 0, "XYStage-StartSpeed": "500.0000", "COM9-StopBits": "2", "405 AO-Description": "NI DAC", "COM4-DelayBetweenCharsMs": "0.0000", "445 AO-Block voltage": "false", "520 AO-TriggerInputLine": "/Dev1/PFI0", "555 AO-Description": "NI DAC", "520 AO-TriggerSampleRate": "100000", "COM4-Verbose": "1", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM4-Name": "COM4", "405 Shutter-Volts": "0.0000", "Vincent-D3-Port": "COM1", "PRIME-PP 4 MIN ADU AFFECTED": "200", "470 Shutter-MinVolts": "0.0000", "PRIME-PP 3 ENABLED": "Yes", "470 AO-Description": "NI DAC", "PRIME-Description": "PVCAM API device adapter", "520 Shutter-MaxVolts": "5.0000", "520 Shutter-Volts": "0.0000", "528 AO-Sequenceable": "1", "PRIME-Binning": "1x1", "555 Shutter-MaxVolts": "5.0000", "528 Shutter-TriggerSequenceLength": "1000", "405 Shutter-Block voltage": "false", "XLIGHT Spin motor-State": "1", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "Vincent-D3-Time to open (ms)": "35.0000", "Vincent-D3-Time to close (ms)": "35.0000", "445 AO-TriggerSampleRate": "100000", "640 Shutter-IOChannel": "Dev1/ao14", "555 AO-Sequenceable": "1", "520 AO-Name": "AnalogIO", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "470 AO-Block voltage": "false", "COM1-Verbose": "1", "COM9-AnswerTimeout": "10000.0000", "528 Shutter-Name": "AnalogIO", "PRIME-Trigger Timeout (secs)": "10", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "NextFrame": 0, "470 AO-TriggerSequenceLength": "1000", "PRIME-TransposeXY": "0", "445 AO-TriggerSequenceLength": "1000", "COM1-Name": "COM1", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "COM9-DataBits": "8", "COM1-AnswerTimeout": "500.0000", "Camera": "", "TE2000-Version": "V2.50-V2.50-V2.10", "528 Shutter-Volts": "0.0000", "XLIGHT Hub-Name": "XLIGHT Hub", "PIEZO-Description": "Ludl stage driver adapter", "PositionName": "Pos17", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSampleRate": "100000", "PRIME-FanSpeedSetpoint": "High", "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "405 AO-TriggerSampleRate": "100000", "470 Shutter-Block voltage": "false", "470-State": "0", "COM3-Parity": "None", "405 AO-Volts": "0.0000", "PRIME-TransposeMirrorY": "0", "PRIME-ChipName": "GS144BSI", "470 AO-Volts": "0.0000", "PRIME-TransposeMirrorX": "0", "445 Shutter-SupportsTriggering": "0", "470 Shutter-SupportsTriggering": "0", "XLIGHT Hub-Description": "XLIGHT hub", "PRIME-PreampOffLimit": "10000", "470 AO-Sequenceable": "1", "528 Shutter-MaxVolts": "5.0000", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "Core-XYStage": "XYStage", "470 AO-TriggerSampleRate": "100000", "PRIME-Timing-PreTriggerDelayNs": "24672000.0000", "528-DA Device": "528 Shutter", "Focus-Description": "Nikon TE2000 focus adapter", "FilterBlock-State": "5", "520 AO-Demo": "No", "COM3-DelayBetweenCharsMs": "0.0000", "470 Shutter-Volts": "0.0000", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "PRIME-PP 2 THRESHOLD": "125", "PRIME-PP 4 THRESHOLD": "75", "XYStage-Acceleration": "4.0000", "470 Shutter-Demo": "No", "PRIME-Name": "PMPCIECam00", "640-DA Device": "640 Shutter", "405 AO-Block voltage": "false", "ElapsedTime-ms": 739972, "PRIME-Offset": "100", "445 AO-IOChannel": "Dev1/ao1", "640 AO-MaxVolts": "5.0000", "PositionIndex": 17, "PRIME-PP 1 ENABLED": "Yes", "COM1-BaudRate": "9600", "640-State": "1", "COM3-Verbose": "1", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "LudlController-TransmissionDelay": "0", "PRIME-FullWellCapacity": "62000", "PRIME-SMARTStreamingEnabled": "No", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "640 Shutter-Description": "NI DAC", "528-Description": "DA device that is used as a shutter", "COM4-Description": "Serial port driver (boost:asio)", "405 Shutter-Description": "NI DAC", "TE2000-Name": "TE2000", "528 Shutter-Description": "NI DAC", "COM9-Description": "Serial port driver (boost:asio)", "LudlController-Name": "LudlController", "445 Shutter-TriggerSequenceLength": "1000", "PRIME-SerialNumber": "A18B203008", "445 AO-Volts": "0.0000", "PRIME-X-dimension": "1200", "PRIME-ShutterCloseDelay": "0", "XLIGHT Disk slider-State": "2", "Position": "Default", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 AO-IOChannel": "Dev1/ao5", "520 AO-IOChannel": "Dev1/ao4", "528 AO-TriggerSequenceLength": "1000", "405 Shutter-MaxVolts": "5.0000", "470-DA Device": "470 Shutter", "PRIME-FTCapable": "No", "Core-ChannelGroup": "Channels", "555 AO-SupportsTriggering": "0", "PRIME-Y-dimension": "1200", "Frame": 0, "555 Shutter-Sequenceable": "1", "PRIME-Timing-ExposureTimeNs": "499998000.0000", "Core-ImageProcessor": "", "528-State": "0", "520 AO-Block voltage": "false", "XLIGHT Excitation Wheel-Label": "QUAD", "640 Shutter-Demo": "No", "COM3-StopBits": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "520 Shutter-IOChannel": "Dev1/ao11", "Width": 1200, "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-SupportsTriggering": "0", "640 AO-TriggerSampleRate": "100000", "520-Description": "DA device that is used as a shutter", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "405 Shutter-Sequenceable": "1", "405 Shutter-SupportsTriggering": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-TriggerSampleRate": "100000", "640 Shutter-MaxVolts": "5.0000", "555 AO-MaxVolts": "5.0000", "520 AO-TriggerSequenceLength": "1000", "COM3-BaudRate": "9600", "COM9-BaudRate": "9600", "Vincent-D3-Channel #": "Ch. #1", "PRIME-PMode": "Normal", "OpticalPath-Label": "Left(100)", "BitDepth": 16, "555 AO-TriggerSequenceLength": "1000", "445 Shutter-Description": "NI DAC", "405 AO-Sequenceable": "1", "PRIME-AcquisitionMethod": "Callbacks", "555 AO-Name": "AnalogIO", "PRIME-ShutterMode": "Pre-Exposure", "COM3-Handshaking": "Off", "445 AO-SupportsTriggering": "0", "ROI": "0-0-1200-1200", "COM9-Verbose": "1", "PRIME-Color": "OFF", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "PRIME-ShutterOpenDelay": "0", "COM1-Parity": "None", "Slice": 6, "SlicePosition": 10, "COM9-DelayBetweenCharsMs": "0.0000", "555-Name": "DA Shutter", "PIEZO-LudlSingleAxisName": "B", "XLIGHT Emission Wheel-Label": "725/40 (C)", "640 Shutter-Name": "AnalogIO", "640 AO-Sequenceable": "1", "520 Shutter-Demo": "No", "528 Shutter-Demo": "No", "470-Name": "DA Shutter", "520-DA Device": "520 Shutter", "XYStage-Description": "Ludl XY stage driver adapter", "445 Shutter-MinVolts": "0.0000", "528 AO-TriggerSampleRate": "100000", "405 AO-MinVolts": "0.0000", "405 Shutter-TriggerSequenceLength": "1000", "Height": 1200, "555 AO-Block voltage": "false", "640 Shutter-Volts": "5.0000", "PixelType": "GRAY16", "445 Shutter-Block voltage": "false", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "520 Shutter-TriggerSequenceLength": "1000", "COM9-Name": "COM9", "Core-SLM": "", "555 AO-Volts": "0.0000", "555 Shutter-Block voltage": "false", "XYStage-Speed": "1800.0000", "520 AO-MaxVolts": "5.0000", "528 Shutter-SupportsTriggering": "0", "COM1-Description": "Serial port driver (boost:asio)", "PRIME-MetadataEnabled": "No", "520 Shutter-MinVolts": "0.0000", "470 Shutter-Sequenceable": "1", "PRIME-PreampDelay": "0", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-MinVolts": "0.0000", "ChannelIndex": 3, "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Core-AutoShutter": "1", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "Exposure-ms": 520, "PRIME-PP 2 ENABLED": "Yes", "640 Shutter-TriggerSampleRate": "100000", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "470 Shutter-IOChannel": "Dev1/ao10", "405 AO-Demo": "No", "PixelSizeUm": 1.1, "470 AO-Demo": "No", "470 Shutter-MaxVolts": "5.0000", "Vincent-D3-Name": "Vincent-D3", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FileName": "img_000000000_Il-C_006.tif", "LudlController-Reset": "Operate", "PRIME-Exposure": "520.00", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "445-Name": "DA Shutter", "555 Shutter-Name": "AnalogIO", "520 Shutter-TriggerSampleRate": "100000", "445 Shutter-IOChannel": "Dev1/ao9", "PRIME-TransposeCorrection": "0", "XYStage-StepSize": "0.1000", "445-Description": "DA device that is used as a shutter", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 AO-IOChannel": "Dev1/ao7", "520 Shutter-Name": "AnalogIO", "COM3-DataBits": "8", "640 AO-Volts": "4.5000", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "555 Shutter-IOChannel": "Dev1/ao13", "WaitInterval": null, "520 Shutter-Sequenceable": "1", "YPositionUm": -13012.300000000001, "445-State": "0", "445-DA Device": "445 Shutter", "PRIME-CircularBufferAutoSize": "ON", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "555 AO-TriggerSampleRate": "100000", "445 Shutter-Volts": "0.0000", "XLIGHT Hub-Port": "COM3", "Core-AutoFocus": "", "COM4-Handshaking": "Off", "528 Shutter-MinVolts": "0.0000", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "445 Shutter-Name": "AnalogIO", "528 Shutter-Sequenceable": "1", "XYStage-TransposeMirrorX": "0", "XYStage-TransposeMirrorY": "0", "PRIME-ReadoutRate": "100MHz 16bit", "XYStage-Name": "Stage", "640 AO-SupportsTriggering": "0", "640-Name": "DA Shutter", "640 Shutter-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "640 AO-Block voltage": "false", "445 Shutter-Sequenceable": "1", "470 AO-MaxVolts": "5.0000", "COM4-BaudRate": "9600", "470-Description": "DA device that is used as a shutter", "520 Shutter-Description": "NI DAC", "PRIME-CCDTemperature": "-14.9200", "Time": "2019-12-17 03:59:56 -0500", "640 AO-TriggerSequenceLength": "1000", "470 AO-Name": "AnalogIO", "XLIGHT Disk slider-Label": "Disk Pos 40um", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "Binning": "1x1", "TE2000-ModelType": "1", "COM3-AnswerTimeout": "500.0000", "555 Shutter-SupportsTriggering": "0", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "TE2000-Port": "COM4", "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Description": "NI DAC", "COM4-AnswerTimeout": "500.0000", "COM1-DelayBetweenCharsMs": "0.0000", "FilterBlock-Label": "Confocal", "445 AO-Name": "AnalogIO", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-Timing-ReadoutTimeNs": "24672000.0000", "528 AO-Demo": "No", "520 AO-Sequenceable": "1", "555-Description": "DA device that is used as a shutter", "405-DA Device": "405 Shutter", "405 AO-TriggerSequenceLength": "1000", "640 AO-MinVolts": "0.0000", "640 Shutter-Block voltage": "false", "FrameIndex": 0, "PRIME-Port": "CMOS", "555 AO-MinVolts": "0.0000", "COM3-Description": "Serial port driver (boost:asio)", "528 AO-Description": "NI DAC", "555 AO-IOChannel": "Dev1/ao6", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "555 AO-TriggerInputLine": "/Dev1/PFI0", "PRIME-ActualInterval-ms": "84.6200", "LudlController-Port": "COM9", "445 Shutter-Demo": "No", "PRIME-Trigger-Expose Out-Mux": "1", "SliceIndex": 6, "PRIME-CCDTemperatureSetPoint": "-15.0000", "405 Shutter-MinVolts": "0.0000", "PRIME-ExposeOutMode": "First Row", "AxisPositions": null, "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Volts": "0.0000", "528-Name": "DA Shutter", "555-State": "0", "XLIGHT Dichroic-Label": "Di03-R635-t1", "445 AO-Sequenceable": "1", "405-Name": "DA Shutter", "405 AO-IOChannel": "Dev1/ao0", "Core-Camera": "PRIME", "Focus-Name": "Focus", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "405 Shutter-IOChannel": "Dev1/ao8", "640 AO-Demo": "No", "640 Shutter-SupportsTriggering": "0", "445 AO-Demo": "No", "555-DA Device": "555 Shutter", "470 Shutter-Description": "NI DAC", "528 AO-Volts": "0.0000", "COM1-DataBits": "8", "Vincent-D3-Address": "x", "520 AO-SupportsTriggering": "0", "405-Description": "DA device that is used as a shutter", "640 Shutter-MinVolts": "0.0000", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "405 AO-Name": "AnalogIO", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "640 AO-TriggerInputLine": "/Dev1/PFI0", "405 AO-TriggerInputLine": "/Dev1/PFI0", "640-Description": "DA device that is used as a shutter", "445 Shutter-TriggerSampleRate": "100000", "470 Shutter-TriggerSequenceLength": "1000", "COM1-StopBits": "1", "520 Shutter-SupportsTriggering": "0", "405-State": "0", "Core-Initialize": "1", "XLIGHT Emission Wheel-State": "3", "520 AO-Description": "NI DAC", "528 AO-TriggerInputLine": "/Dev1/PFI0", "445 Shutter-MaxVolts": "5.0000", "COM9-Handshaking": "Off", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "XLIGHT Spin motor-Label": "On (spinning)", "PRIME-PP 5 ENABLED": "No", "UUID": "454d4a93-601c-4203-a49f-88cd7446066a", "PRIME-TriggerMode": "Internal Trigger", "COM1-Handshaking": "Off", "PRIME-Timing-PostTriggerDelayNs": "49344000.0000", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "Focus-Position": "16732.9000", "528 Shutter-Block voltage": "false", "Channel": "Il-C", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "555 Shutter-Demo": "No", "405 Shutter-Demo": "No", "528 AO-Name": "AnalogIO", "520-State": "0", "Core-Galvo": "", "405 Shutter-TriggerSampleRate": "100000", "Focus-FocusStepSize_(nm)": "100", "PRIME-PixelType": "16bit", "COM4-DataBits": "8", "470 AO-IOChannel": "Dev1/ao2", "520 AO-MinVolts": "0.0000", "COM9-Parity": "None", "PRIME-CircularBufferEnabled": "ON", "PRIME-PP 6 Reset": "No", "640 AO-Description": "NI DAC", "Source": "PRIME", "445 AO-MinVolts": "0.0000", "PIEZO-Name": "Stage", "445 AO-Description": "NI DAC", "PRIME-CircularBufferFrameCount": "46", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "520 AO-Volts": "0.0000", "528 Shutter-TriggerSampleRate": "100000", "XPositionUm": 23269.100000000002, "OpticalPath-State": "4", "PRIME-PP 3 THRESHOLD": "80", "PRIME-PVCAM Version": "3.7.1", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Gain": "1-HDR", "640 Shutter-TriggerSequenceLength": "1000", "528 AO-SupportsTriggering": "0", "445 AO-TriggerInputLine": "/Dev1/PFI0", "OpticalPath-Name": "OpticalPath", "PRIME-PP 4 ENABLED": "Yes", "PRIME-PVCAM Adapter Version": "1.3.29", "COM3-Name": "COM3", "405 AO-MaxVolts": "5.0000", "520 Shutter-Block voltage": "false", "PRIME-Timing-ClearingTimeNs": "49344000.0000", "Core-Focus": "PIEZO", "528 AO-Block voltage": "false", "TE2000-Description": "Nikon TE2000 hub", "528 AO-MinVolts": "0.0000", "COM4-Parity": "None", "Summary": { "Slices": 11, "ChContrastMin": [ 0, 0, 0, 0, 0, 0 ], "PixelAspect": 1, "SlicesFirst": true, "Interval_ms": 0, "Time": "2019-12-17 03:59:33 -0500", "Prefix": "seq_7", "InitialPositionList": [ { "Label": "Pos0", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [149.91], "Focus": [16657.7], "XYStage": [ 71629.7, 19152.9 ] }, "GridColumnIndex": 0 }, { "Label": "Pos1", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [190.82], "Focus": [16485.4], "XYStage": [ 65702.3, 19396.600000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos2", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [194.69], "Focus": [16446.8], "XYStage": [ 59880.5, 19007.8 ] }, "GridColumnIndex": 0 }, { "Label": "Pos3", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [145.04], "Focus": [16446.6], "XYStage": [ 53616.9, 18818.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos4", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [169.57500000000002], "Focus": [16446.5], "XYStage": [ 47662.8, 18817.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos5", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [131.805], "Focus": [16446.4], "XYStage": [ 41918.8, 18298.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos6", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [113.145], "Focus": [16446.5], "XYStage": [ 36129.9, 18895.8 ] }, "GridColumnIndex": 0 }, { "Label": "Pos7", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [141.005], "Focus": [16446.6], "XYStage": [ 30299.100000000002, 18895.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos8", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [140.97], "Focus": [16446.5], "XYStage": [ 23952.100000000002, 19156 ] }, "GridColumnIndex": 0 }, { "Label": "Pos9", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [184.57500000000002], "Focus": [16677.9], "XYStage": [ 72866.8, -14809.300000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos10", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [167.95000000000002], "Focus": [16557.9], "XYStage": [ 66515.6, -14371.300000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos11", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [168], "Focus": [16618.9], "XYStage": [ 59724.3, -13964.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos12", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [203.205], "Focus": [16779.6], "XYStage": [ 53862.3, -13494.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos13", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [203.20000000000002], "Focus": [16749], "XYStage": [ 48108.3, -13805.400000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos14", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [203.19], "Focus": [16675.9], "XYStage": [ 41645, -13804.900000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos15", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [203.21], "Focus": [16675.7], "XYStage": [ 35352.6, -13805.6 ] }, "GridColumnIndex": 0 }, { "Label": "Pos16", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [203.225], "Focus": [16675.8], "XYStage": [ 29298, -13447.400000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos17", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [203.215], "Focus": [16675.8], "XYStage": [ 23269.100000000002, -13012.300000000001 ] }, "GridColumnIndex": 0 } ], "z-step_um": 10, "GridRow": 0, "BitDepth": 16, "Depth": 2, "Channels": 6, "Source": "Micro-Manager", "ChColors": [ -16711681, -256, -65281, -1, -1, -16776961 ], "MetadataVersion": 10, "KeepShutterOpenChannels": true, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536, 65536 ], "CustomIntervals_ms": [], "Height": 1200, "MicroManagerVersion": "1.4.23 20180219", "GridColumn": 0, "UUID": "3ad8801b-5699-40d6-813b-4ad840863e38", "PixelType": "GRAY16", "Width": 1200, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\Yan\\20191216-YC113-S11S12-miseq", "Frames": 1, "Comment": "", "UserName": "Nikon", "PixelSize_um": 1.1, "ROI": [ 0, 0, 1200, 1200 ], "Date": "2019-12-17", "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC", "P-DAPI-high" ], "KeepShutterOpenSlices": true, "IJType": 1, "ComputerName": "ZadorConfocal2", "Positions": 18, "PositionIndex": 17, "CameraTimeout": 20000, "TimeFirst": false }, "640 AO-Name": "AnalogIO", "Vincent-D3-Command": "Close" }, "FrameKey-0-3-7": { "COM4-StopBits": "1", "528 AO-MaxVolts": "5.0000", "XLIGHT Excitation Wheel-State": "1", "470 Shutter-Name": "AnalogIO", "Core-Shutter": "640", "555 AO-Demo": "No", "XLIGHT Dichroic-State": "2", "XYStage-StepSize-Y": "0.1000", "ZPositionUm": 223.215, "XYStage-StepSize-X": "0.1000", "PRIME-FirmwareVersion": "20.28", "PRIME-ClearCycles": "2", "520-Name": "DA Shutter", "405 Shutter-Name": "AnalogIO", "PIEZO-StepSize": "0.0050", "PRIME-ClearMode": "Pre-Exposure", "470 AO-MinVolts": "0.0000", "405 AO-SupportsTriggering": "1", "CameraChannelIndex": 0, "XYStage-StartSpeed": "500.0000", "COM9-StopBits": "2", "405 AO-Description": "NI DAC", "COM4-DelayBetweenCharsMs": "0.0000", "445 AO-Block voltage": "false", "520 AO-TriggerInputLine": "/Dev1/PFI0", "555 AO-Description": "NI DAC", "520 AO-TriggerSampleRate": "100000", "COM4-Verbose": "1", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM4-Name": "COM4", "405 Shutter-Volts": "0.0000", "Vincent-D3-Port": "COM1", "PRIME-PP 4 MIN ADU AFFECTED": "200", "470 Shutter-MinVolts": "0.0000", "PRIME-PP 3 ENABLED": "Yes", "470 AO-Description": "NI DAC", "PRIME-Description": "PVCAM API device adapter", "520 Shutter-MaxVolts": "5.0000", "520 Shutter-Volts": "0.0000", "528 AO-Sequenceable": "1", "PRIME-Binning": "1x1", "555 Shutter-MaxVolts": "5.0000", "528 Shutter-TriggerSequenceLength": "1000", "405 Shutter-Block voltage": "false", "XLIGHT Spin motor-State": "1", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "Vincent-D3-Time to open (ms)": "35.0000", "Vincent-D3-Time to close (ms)": "35.0000", "445 AO-TriggerSampleRate": "100000", "640 Shutter-IOChannel": "Dev1/ao14", "555 AO-Sequenceable": "1", "520 AO-Name": "AnalogIO", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "470 AO-Block voltage": "false", "COM1-Verbose": "1", "COM9-AnswerTimeout": "10000.0000", "528 Shutter-Name": "AnalogIO", "PRIME-Trigger Timeout (secs)": "10", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "NextFrame": 0, "470 AO-TriggerSequenceLength": "1000", "PRIME-TransposeXY": "0", "445 AO-TriggerSequenceLength": "1000", "COM1-Name": "COM1", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "COM9-DataBits": "8", "COM1-AnswerTimeout": "500.0000", "Camera": "", "TE2000-Version": "V2.50-V2.50-V2.10", "528 Shutter-Volts": "0.0000", "XLIGHT Hub-Name": "XLIGHT Hub", "PIEZO-Description": "Ludl stage driver adapter", "PositionName": "Pos17", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSampleRate": "100000", "PRIME-FanSpeedSetpoint": "High", "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "405 AO-TriggerSampleRate": "100000", "470 Shutter-Block voltage": "false", "470-State": "0", "COM3-Parity": "None", "405 AO-Volts": "0.0000", "PRIME-TransposeMirrorY": "0", "PRIME-ChipName": "GS144BSI", "470 AO-Volts": "0.0000", "PRIME-TransposeMirrorX": "0", "445 Shutter-SupportsTriggering": "0", "470 Shutter-SupportsTriggering": "0", "XLIGHT Hub-Description": "XLIGHT hub", "PRIME-PreampOffLimit": "10000", "470 AO-Sequenceable": "1", "528 Shutter-MaxVolts": "5.0000", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "Core-XYStage": "XYStage", "470 AO-TriggerSampleRate": "100000", "PRIME-Timing-PreTriggerDelayNs": "24672000.0000", "528-DA Device": "528 Shutter", "Focus-Description": "Nikon TE2000 focus adapter", "FilterBlock-State": "5", "520 AO-Demo": "No", "COM3-DelayBetweenCharsMs": "0.0000", "470 Shutter-Volts": "0.0000", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "PRIME-PP 2 THRESHOLD": "125", "PRIME-PP 4 THRESHOLD": "75", "XYStage-Acceleration": "4.0000", "470 Shutter-Demo": "No", "PRIME-Name": "PMPCIECam00", "640-DA Device": "640 Shutter", "405 AO-Block voltage": "false", "ElapsedTime-ms": 740743, "PRIME-Offset": "100", "445 AO-IOChannel": "Dev1/ao1", "640 AO-MaxVolts": "5.0000", "PositionIndex": 17, "PRIME-PP 1 ENABLED": "Yes", "COM1-BaudRate": "9600", "640-State": "1", "COM3-Verbose": "1", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "LudlController-TransmissionDelay": "0", "PRIME-FullWellCapacity": "62000", "PRIME-SMARTStreamingEnabled": "No", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "640 Shutter-Description": "NI DAC", "528-Description": "DA device that is used as a shutter", "COM4-Description": "Serial port driver (boost:asio)", "405 Shutter-Description": "NI DAC", "TE2000-Name": "TE2000", "528 Shutter-Description": "NI DAC", "COM9-Description": "Serial port driver (boost:asio)", "LudlController-Name": "LudlController", "445 Shutter-TriggerSequenceLength": "1000", "PRIME-SerialNumber": "A18B203008", "445 AO-Volts": "0.0000", "PRIME-X-dimension": "1200", "PRIME-ShutterCloseDelay": "0", "XLIGHT Disk slider-State": "2", "Position": "Default", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 AO-IOChannel": "Dev1/ao5", "520 AO-IOChannel": "Dev1/ao4", "528 AO-TriggerSequenceLength": "1000", "405 Shutter-MaxVolts": "5.0000", "470-DA Device": "470 Shutter", "PRIME-FTCapable": "No", "Core-ChannelGroup": "Channels", "555 AO-SupportsTriggering": "0", "PRIME-Y-dimension": "1200", "Frame": 0, "555 Shutter-Sequenceable": "1", "PRIME-Timing-ExposureTimeNs": "499998000.0000", "Core-ImageProcessor": "", "528-State": "0", "520 AO-Block voltage": "false", "XLIGHT Excitation Wheel-Label": "QUAD", "640 Shutter-Demo": "No", "COM3-StopBits": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "520 Shutter-IOChannel": "Dev1/ao11", "Width": 1200, "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-SupportsTriggering": "0", "640 AO-TriggerSampleRate": "100000", "520-Description": "DA device that is used as a shutter", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "405 Shutter-Sequenceable": "1", "405 Shutter-SupportsTriggering": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-TriggerSampleRate": "100000", "640 Shutter-MaxVolts": "5.0000", "555 AO-MaxVolts": "5.0000", "520 AO-TriggerSequenceLength": "1000", "COM3-BaudRate": "9600", "COM9-BaudRate": "9600", "Vincent-D3-Channel #": "Ch. #1", "PRIME-PMode": "Normal", "OpticalPath-Label": "Left(100)", "BitDepth": 16, "555 AO-TriggerSequenceLength": "1000", "445 Shutter-Description": "NI DAC", "405 AO-Sequenceable": "1", "PRIME-AcquisitionMethod": "Callbacks", "555 AO-Name": "AnalogIO", "PRIME-ShutterMode": "Pre-Exposure", "COM3-Handshaking": "Off", "445 AO-SupportsTriggering": "0", "ROI": "0-0-1200-1200", "COM9-Verbose": "1", "PRIME-Color": "OFF", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "PRIME-ShutterOpenDelay": "0", "COM1-Parity": "None", "Slice": 7, "SlicePosition": 20, "COM9-DelayBetweenCharsMs": "0.0000", "555-Name": "DA Shutter", "PIEZO-LudlSingleAxisName": "B", "XLIGHT Emission Wheel-Label": "725/40 (C)", "640 Shutter-Name": "AnalogIO", "640 AO-Sequenceable": "1", "520 Shutter-Demo": "No", "528 Shutter-Demo": "No", "470-Name": "DA Shutter", "520-DA Device": "520 Shutter", "XYStage-Description": "Ludl XY stage driver adapter", "445 Shutter-MinVolts": "0.0000", "528 AO-TriggerSampleRate": "100000", "405 AO-MinVolts": "0.0000", "405 Shutter-TriggerSequenceLength": "1000", "Height": 1200, "555 AO-Block voltage": "false", "640 Shutter-Volts": "5.0000", "PixelType": "GRAY16", "445 Shutter-Block voltage": "false", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "520 Shutter-TriggerSequenceLength": "1000", "COM9-Name": "COM9", "Core-SLM": "", "555 AO-Volts": "0.0000", "555 Shutter-Block voltage": "false", "XYStage-Speed": "1800.0000", "520 AO-MaxVolts": "5.0000", "528 Shutter-SupportsTriggering": "0", "COM1-Description": "Serial port driver (boost:asio)", "PRIME-MetadataEnabled": "No", "520 Shutter-MinVolts": "0.0000", "470 Shutter-Sequenceable": "1", "PRIME-PreampDelay": "0", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-MinVolts": "0.0000", "ChannelIndex": 3, "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Core-AutoShutter": "1", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "Exposure-ms": 520, "PRIME-PP 2 ENABLED": "Yes", "640 Shutter-TriggerSampleRate": "100000", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "470 Shutter-IOChannel": "Dev1/ao10", "405 AO-Demo": "No", "PixelSizeUm": 1.1, "470 AO-Demo": "No", "470 Shutter-MaxVolts": "5.0000", "Vincent-D3-Name": "Vincent-D3", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FileName": "img_000000000_Il-C_007.tif", "LudlController-Reset": "Operate", "PRIME-Exposure": "520.00", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "445-Name": "DA Shutter", "555 Shutter-Name": "AnalogIO", "520 Shutter-TriggerSampleRate": "100000", "445 Shutter-IOChannel": "Dev1/ao9", "PRIME-TransposeCorrection": "0", "XYStage-StepSize": "0.1000", "445-Description": "DA device that is used as a shutter", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 AO-IOChannel": "Dev1/ao7", "520 Shutter-Name": "AnalogIO", "COM3-DataBits": "8", "640 AO-Volts": "4.5000", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "555 Shutter-IOChannel": "Dev1/ao13", "WaitInterval": null, "520 Shutter-Sequenceable": "1", "YPositionUm": -13012.300000000001, "445-State": "0", "445-DA Device": "445 Shutter", "PRIME-CircularBufferAutoSize": "ON", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "555 AO-TriggerSampleRate": "100000", "445 Shutter-Volts": "0.0000", "XLIGHT Hub-Port": "COM3", "Core-AutoFocus": "", "COM4-Handshaking": "Off", "528 Shutter-MinVolts": "0.0000", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "445 Shutter-Name": "AnalogIO", "528 Shutter-Sequenceable": "1", "XYStage-TransposeMirrorX": "0", "XYStage-TransposeMirrorY": "0", "PRIME-ReadoutRate": "100MHz 16bit", "XYStage-Name": "Stage", "640 AO-SupportsTriggering": "0", "640-Name": "DA Shutter", "640 Shutter-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "640 AO-Block voltage": "false", "445 Shutter-Sequenceable": "1", "470 AO-MaxVolts": "5.0000", "COM4-BaudRate": "9600", "470-Description": "DA device that is used as a shutter", "520 Shutter-Description": "NI DAC", "PRIME-CCDTemperature": "-14.9200", "Time": "2019-12-17 03:59:57 -0500", "640 AO-TriggerSequenceLength": "1000", "470 AO-Name": "AnalogIO", "XLIGHT Disk slider-Label": "Disk Pos 40um", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "Binning": "1x1", "TE2000-ModelType": "1", "COM3-AnswerTimeout": "500.0000", "555 Shutter-SupportsTriggering": "0", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "TE2000-Port": "COM4", "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Description": "NI DAC", "COM4-AnswerTimeout": "500.0000", "COM1-DelayBetweenCharsMs": "0.0000", "FilterBlock-Label": "Confocal", "445 AO-Name": "AnalogIO", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-Timing-ReadoutTimeNs": "24672000.0000", "528 AO-Demo": "No", "520 AO-Sequenceable": "1", "555-Description": "DA device that is used as a shutter", "405-DA Device": "405 Shutter", "405 AO-TriggerSequenceLength": "1000", "640 AO-MinVolts": "0.0000", "640 Shutter-Block voltage": "false", "FrameIndex": 0, "PRIME-Port": "CMOS", "555 AO-MinVolts": "0.0000", "COM3-Description": "Serial port driver (boost:asio)", "528 AO-Description": "NI DAC", "555 AO-IOChannel": "Dev1/ao6", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "555 AO-TriggerInputLine": "/Dev1/PFI0", "PRIME-ActualInterval-ms": "84.6200", "LudlController-Port": "COM9", "445 Shutter-Demo": "No", "PRIME-Trigger-Expose Out-Mux": "1", "SliceIndex": 7, "PRIME-CCDTemperatureSetPoint": "-15.0000", "405 Shutter-MinVolts": "0.0000", "PRIME-ExposeOutMode": "First Row", "AxisPositions": null, "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Volts": "0.0000", "528-Name": "DA Shutter", "555-State": "0", "XLIGHT Dichroic-Label": "Di03-R635-t1", "445 AO-Sequenceable": "1", "405-Name": "DA Shutter", "405 AO-IOChannel": "Dev1/ao0", "Core-Camera": "PRIME", "Focus-Name": "Focus", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "405 Shutter-IOChannel": "Dev1/ao8", "640 AO-Demo": "No", "640 Shutter-SupportsTriggering": "0", "445 AO-Demo": "No", "555-DA Device": "555 Shutter", "470 Shutter-Description": "NI DAC", "528 AO-Volts": "0.0000", "COM1-DataBits": "8", "Vincent-D3-Address": "x", "520 AO-SupportsTriggering": "0", "405-Description": "DA device that is used as a shutter", "640 Shutter-MinVolts": "0.0000", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "405 AO-Name": "AnalogIO", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "640 AO-TriggerInputLine": "/Dev1/PFI0", "405 AO-TriggerInputLine": "/Dev1/PFI0", "640-Description": "DA device that is used as a shutter", "445 Shutter-TriggerSampleRate": "100000", "470 Shutter-TriggerSequenceLength": "1000", "COM1-StopBits": "1", "520 Shutter-SupportsTriggering": "0", "405-State": "0", "Core-Initialize": "1", "XLIGHT Emission Wheel-State": "3", "520 AO-Description": "NI DAC", "528 AO-TriggerInputLine": "/Dev1/PFI0", "445 Shutter-MaxVolts": "5.0000", "COM9-Handshaking": "Off", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "XLIGHT Spin motor-Label": "On (spinning)", "PRIME-PP 5 ENABLED": "No", "UUID": "d20fb1ce-c791-47c3-9391-60eb483a6f20", "PRIME-TriggerMode": "Internal Trigger", "COM1-Handshaking": "Off", "PRIME-Timing-PostTriggerDelayNs": "49344000.0000", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "Focus-Position": "16732.9000", "528 Shutter-Block voltage": "false", "Channel": "Il-C", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "555 Shutter-Demo": "No", "405 Shutter-Demo": "No", "528 AO-Name": "AnalogIO", "520-State": "0", "Core-Galvo": "", "405 Shutter-TriggerSampleRate": "100000", "Focus-FocusStepSize_(nm)": "100", "PRIME-PixelType": "16bit", "COM4-DataBits": "8", "470 AO-IOChannel": "Dev1/ao2", "520 AO-MinVolts": "0.0000", "COM9-Parity": "None", "PRIME-CircularBufferEnabled": "ON", "PRIME-PP 6 Reset": "No", "640 AO-Description": "NI DAC", "Source": "PRIME", "445 AO-MinVolts": "0.0000", "PIEZO-Name": "Stage", "445 AO-Description": "NI DAC", "PRIME-CircularBufferFrameCount": "46", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "520 AO-Volts": "0.0000", "528 Shutter-TriggerSampleRate": "100000", "XPositionUm": 23269.100000000002, "OpticalPath-State": "4", "PRIME-PP 3 THRESHOLD": "80", "PRIME-PVCAM Version": "3.7.1", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Gain": "1-HDR", "640 Shutter-TriggerSequenceLength": "1000", "528 AO-SupportsTriggering": "0", "445 AO-TriggerInputLine": "/Dev1/PFI0", "OpticalPath-Name": "OpticalPath", "PRIME-PP 4 ENABLED": "Yes", "PRIME-PVCAM Adapter Version": "1.3.29", "COM3-Name": "COM3", "405 AO-MaxVolts": "5.0000", "520 Shutter-Block voltage": "false", "PRIME-Timing-ClearingTimeNs": "49344000.0000", "Core-Focus": "PIEZO", "528 AO-Block voltage": "false", "TE2000-Description": "Nikon TE2000 hub", "528 AO-MinVolts": "0.0000", "COM4-Parity": "None", "Summary": { "Slices": 11, "ChContrastMin": [ 0, 0, 0, 0, 0, 0 ], "PixelAspect": 1, "SlicesFirst": true, "Interval_ms": 0, "Time": "2019-12-17 03:59:33 -0500", "Prefix": "seq_7", "InitialPositionList": [ { "Label": "Pos0", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [149.91], "Focus": [16657.7], "XYStage": [ 71629.7, 19152.9 ] }, "GridColumnIndex": 0 }, { "Label": "Pos1", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [190.82], "Focus": [16485.4], "XYStage": [ 65702.3, 19396.600000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos2", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [194.69], "Focus": [16446.8], "XYStage": [ 59880.5, 19007.8 ] }, "GridColumnIndex": 0 }, { "Label": "Pos3", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [145.04], "Focus": [16446.6], "XYStage": [ 53616.9, 18818.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos4", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [169.57500000000002], "Focus": [16446.5], "XYStage": [ 47662.8, 18817.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos5", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [131.805], "Focus": [16446.4], "XYStage": [ 41918.8, 18298.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos6", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [113.145], "Focus": [16446.5], "XYStage": [ 36129.9, 18895.8 ] }, "GridColumnIndex": 0 }, { "Label": "Pos7", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [141.005], "Focus": [16446.6], "XYStage": [ 30299.100000000002, 18895.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos8", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [140.97], "Focus": [16446.5], "XYStage": [ 23952.100000000002, 19156 ] }, "GridColumnIndex": 0 }, { "Label": "Pos9", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [184.57500000000002], "Focus": [16677.9], "XYStage": [ 72866.8, -14809.300000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos10", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [167.95000000000002], "Focus": [16557.9], "XYStage": [ 66515.6, -14371.300000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos11", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [168], "Focus": [16618.9], "XYStage": [ 59724.3, -13964.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos12", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [203.205], "Focus": [16779.6], "XYStage": [ 53862.3, -13494.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos13", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [203.20000000000002], "Focus": [16749], "XYStage": [ 48108.3, -13805.400000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos14", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [203.19], "Focus": [16675.9], "XYStage": [ 41645, -13804.900000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos15", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [203.21], "Focus": [16675.7], "XYStage": [ 35352.6, -13805.6 ] }, "GridColumnIndex": 0 }, { "Label": "Pos16", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [203.225], "Focus": [16675.8], "XYStage": [ 29298, -13447.400000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos17", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [203.215], "Focus": [16675.8], "XYStage": [ 23269.100000000002, -13012.300000000001 ] }, "GridColumnIndex": 0 } ], "z-step_um": 10, "GridRow": 0, "BitDepth": 16, "Depth": 2, "Channels": 6, "Source": "Micro-Manager", "ChColors": [ -16711681, -256, -65281, -1, -1, -16776961 ], "MetadataVersion": 10, "KeepShutterOpenChannels": true, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536, 65536 ], "CustomIntervals_ms": [], "Height": 1200, "MicroManagerVersion": "1.4.23 20180219", "GridColumn": 0, "UUID": "3ad8801b-5699-40d6-813b-4ad840863e38", "PixelType": "GRAY16", "Width": 1200, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\Yan\\20191216-YC113-S11S12-miseq", "Frames": 1, "Comment": "", "UserName": "Nikon", "PixelSize_um": 1.1, "ROI": [ 0, 0, 1200, 1200 ], "Date": "2019-12-17", "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC", "P-DAPI-high" ], "KeepShutterOpenSlices": true, "IJType": 1, "ComputerName": "ZadorConfocal2", "Positions": 18, "PositionIndex": 17, "CameraTimeout": 20000, "TimeFirst": false }, "640 AO-Name": "AnalogIO", "Vincent-D3-Command": "Close" }, "FrameKey-0-3-8": { "COM4-StopBits": "1", "528 AO-MaxVolts": "5.0000", "XLIGHT Excitation Wheel-State": "1", "470 Shutter-Name": "AnalogIO", "Core-Shutter": "640", "555 AO-Demo": "No", "XLIGHT Dichroic-State": "2", "XYStage-StepSize-Y": "0.1000", "ZPositionUm": 233.215, "XYStage-StepSize-X": "0.1000", "PRIME-FirmwareVersion": "20.28", "PRIME-ClearCycles": "2", "520-Name": "DA Shutter", "405 Shutter-Name": "AnalogIO", "PIEZO-StepSize": "0.0050", "PRIME-ClearMode": "Pre-Exposure", "470 AO-MinVolts": "0.0000", "405 AO-SupportsTriggering": "1", "CameraChannelIndex": 0, "XYStage-StartSpeed": "500.0000", "COM9-StopBits": "2", "405 AO-Description": "NI DAC", "COM4-DelayBetweenCharsMs": "0.0000", "445 AO-Block voltage": "false", "520 AO-TriggerInputLine": "/Dev1/PFI0", "555 AO-Description": "NI DAC", "520 AO-TriggerSampleRate": "100000", "COM4-Verbose": "1", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM4-Name": "COM4", "405 Shutter-Volts": "0.0000", "Vincent-D3-Port": "COM1", "PRIME-PP 4 MIN ADU AFFECTED": "200", "470 Shutter-MinVolts": "0.0000", "PRIME-PP 3 ENABLED": "Yes", "470 AO-Description": "NI DAC", "PRIME-Description": "PVCAM API device adapter", "520 Shutter-MaxVolts": "5.0000", "520 Shutter-Volts": "0.0000", "528 AO-Sequenceable": "1", "PRIME-Binning": "1x1", "555 Shutter-MaxVolts": "5.0000", "528 Shutter-TriggerSequenceLength": "1000", "405 Shutter-Block voltage": "false", "XLIGHT Spin motor-State": "1", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "Vincent-D3-Time to open (ms)": "35.0000", "Vincent-D3-Time to close (ms)": "35.0000", "445 AO-TriggerSampleRate": "100000", "640 Shutter-IOChannel": "Dev1/ao14", "555 AO-Sequenceable": "1", "520 AO-Name": "AnalogIO", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "470 AO-Block voltage": "false", "COM1-Verbose": "1", "COM9-AnswerTimeout": "10000.0000", "528 Shutter-Name": "AnalogIO", "PRIME-Trigger Timeout (secs)": "10", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "NextFrame": 0, "470 AO-TriggerSequenceLength": "1000", "PRIME-TransposeXY": "0", "445 AO-TriggerSequenceLength": "1000", "COM1-Name": "COM1", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "COM9-DataBits": "8", "COM1-AnswerTimeout": "500.0000", "Camera": "", "TE2000-Version": "V2.50-V2.50-V2.10", "528 Shutter-Volts": "0.0000", "XLIGHT Hub-Name": "XLIGHT Hub", "PIEZO-Description": "Ludl stage driver adapter", "PositionName": "Pos17", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSampleRate": "100000", "PRIME-FanSpeedSetpoint": "High", "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "405 AO-TriggerSampleRate": "100000", "470 Shutter-Block voltage": "false", "470-State": "0", "COM3-Parity": "None", "405 AO-Volts": "0.0000", "PRIME-TransposeMirrorY": "0", "PRIME-ChipName": "GS144BSI", "470 AO-Volts": "0.0000", "PRIME-TransposeMirrorX": "0", "445 Shutter-SupportsTriggering": "0", "470 Shutter-SupportsTriggering": "0", "XLIGHT Hub-Description": "XLIGHT hub", "PRIME-PreampOffLimit": "10000", "470 AO-Sequenceable": "1", "528 Shutter-MaxVolts": "5.0000", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "Core-XYStage": "XYStage", "470 AO-TriggerSampleRate": "100000", "PRIME-Timing-PreTriggerDelayNs": "24672000.0000", "528-DA Device": "528 Shutter", "Focus-Description": "Nikon TE2000 focus adapter", "FilterBlock-State": "5", "520 AO-Demo": "No", "COM3-DelayBetweenCharsMs": "0.0000", "470 Shutter-Volts": "0.0000", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "PRIME-PP 2 THRESHOLD": "125", "PRIME-PP 4 THRESHOLD": "75", "XYStage-Acceleration": "4.0000", "470 Shutter-Demo": "No", "PRIME-Name": "PMPCIECam00", "640-DA Device": "640 Shutter", "405 AO-Block voltage": "false", "ElapsedTime-ms": 741510, "PRIME-Offset": "100", "445 AO-IOChannel": "Dev1/ao1", "640 AO-MaxVolts": "5.0000", "PositionIndex": 17, "PRIME-PP 1 ENABLED": "Yes", "COM1-BaudRate": "9600", "640-State": "1", "COM3-Verbose": "1", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "LudlController-TransmissionDelay": "0", "PRIME-FullWellCapacity": "62000", "PRIME-SMARTStreamingEnabled": "No", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "640 Shutter-Description": "NI DAC", "528-Description": "DA device that is used as a shutter", "COM4-Description": "Serial port driver (boost:asio)", "405 Shutter-Description": "NI DAC", "TE2000-Name": "TE2000", "528 Shutter-Description": "NI DAC", "COM9-Description": "Serial port driver (boost:asio)", "LudlController-Name": "LudlController", "445 Shutter-TriggerSequenceLength": "1000", "PRIME-SerialNumber": "A18B203008", "445 AO-Volts": "0.0000", "PRIME-X-dimension": "1200", "PRIME-ShutterCloseDelay": "0", "XLIGHT Disk slider-State": "2", "Position": "Default", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 AO-IOChannel": "Dev1/ao5", "520 AO-IOChannel": "Dev1/ao4", "528 AO-TriggerSequenceLength": "1000", "405 Shutter-MaxVolts": "5.0000", "470-DA Device": "470 Shutter", "PRIME-FTCapable": "No", "Core-ChannelGroup": "Channels", "555 AO-SupportsTriggering": "0", "PRIME-Y-dimension": "1200", "Frame": 0, "555 Shutter-Sequenceable": "1", "PRIME-Timing-ExposureTimeNs": "499998000.0000", "Core-ImageProcessor": "", "528-State": "0", "520 AO-Block voltage": "false", "XLIGHT Excitation Wheel-Label": "QUAD", "640 Shutter-Demo": "No", "COM3-StopBits": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "520 Shutter-IOChannel": "Dev1/ao11", "Width": 1200, "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-SupportsTriggering": "0", "640 AO-TriggerSampleRate": "100000", "520-Description": "DA device that is used as a shutter", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "405 Shutter-Sequenceable": "1", "405 Shutter-SupportsTriggering": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-TriggerSampleRate": "100000", "640 Shutter-MaxVolts": "5.0000", "555 AO-MaxVolts": "5.0000", "520 AO-TriggerSequenceLength": "1000", "COM3-BaudRate": "9600", "COM9-BaudRate": "9600", "Vincent-D3-Channel #": "Ch. #1", "PRIME-PMode": "Normal", "OpticalPath-Label": "Left(100)", "BitDepth": 16, "555 AO-TriggerSequenceLength": "1000", "445 Shutter-Description": "NI DAC", "405 AO-Sequenceable": "1", "PRIME-AcquisitionMethod": "Callbacks", "555 AO-Name": "AnalogIO", "PRIME-ShutterMode": "Pre-Exposure", "COM3-Handshaking": "Off", "445 AO-SupportsTriggering": "0", "ROI": "0-0-1200-1200", "COM9-Verbose": "1", "PRIME-Color": "OFF", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "PRIME-ShutterOpenDelay": "0", "COM1-Parity": "None", "Slice": 8, "SlicePosition": 30, "COM9-DelayBetweenCharsMs": "0.0000", "555-Name": "DA Shutter", "PIEZO-LudlSingleAxisName": "B", "XLIGHT Emission Wheel-Label": "725/40 (C)", "640 Shutter-Name": "AnalogIO", "640 AO-Sequenceable": "1", "520 Shutter-Demo": "No", "528 Shutter-Demo": "No", "470-Name": "DA Shutter", "520-DA Device": "520 Shutter", "XYStage-Description": "Ludl XY stage driver adapter", "445 Shutter-MinVolts": "0.0000", "528 AO-TriggerSampleRate": "100000", "405 AO-MinVolts": "0.0000", "405 Shutter-TriggerSequenceLength": "1000", "Height": 1200, "555 AO-Block voltage": "false", "640 Shutter-Volts": "5.0000", "PixelType": "GRAY16", "445 Shutter-Block voltage": "false", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "520 Shutter-TriggerSequenceLength": "1000", "COM9-Name": "COM9", "Core-SLM": "", "555 AO-Volts": "0.0000", "555 Shutter-Block voltage": "false", "XYStage-Speed": "1800.0000", "520 AO-MaxVolts": "5.0000", "528 Shutter-SupportsTriggering": "0", "COM1-Description": "Serial port driver (boost:asio)", "PRIME-MetadataEnabled": "No", "520 Shutter-MinVolts": "0.0000", "470 Shutter-Sequenceable": "1", "PRIME-PreampDelay": "0", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-MinVolts": "0.0000", "ChannelIndex": 3, "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Core-AutoShutter": "1", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "Exposure-ms": 520, "PRIME-PP 2 ENABLED": "Yes", "640 Shutter-TriggerSampleRate": "100000", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "470 Shutter-IOChannel": "Dev1/ao10", "405 AO-Demo": "No", "PixelSizeUm": 1.1, "470 AO-Demo": "No", "470 Shutter-MaxVolts": "5.0000", "Vincent-D3-Name": "Vincent-D3", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FileName": "img_000000000_Il-C_008.tif", "LudlController-Reset": "Operate", "PRIME-Exposure": "520.00", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "445-Name": "DA Shutter", "555 Shutter-Name": "AnalogIO", "520 Shutter-TriggerSampleRate": "100000", "445 Shutter-IOChannel": "Dev1/ao9", "PRIME-TransposeCorrection": "0", "XYStage-StepSize": "0.1000", "445-Description": "DA device that is used as a shutter", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 AO-IOChannel": "Dev1/ao7", "520 Shutter-Name": "AnalogIO", "COM3-DataBits": "8", "640 AO-Volts": "4.5000", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "555 Shutter-IOChannel": "Dev1/ao13", "WaitInterval": null, "520 Shutter-Sequenceable": "1", "YPositionUm": -13012.300000000001, "445-State": "0", "445-DA Device": "445 Shutter", "PRIME-CircularBufferAutoSize": "ON", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "555 AO-TriggerSampleRate": "100000", "445 Shutter-Volts": "0.0000", "XLIGHT Hub-Port": "COM3", "Core-AutoFocus": "", "COM4-Handshaking": "Off", "528 Shutter-MinVolts": "0.0000", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "445 Shutter-Name": "AnalogIO", "528 Shutter-Sequenceable": "1", "XYStage-TransposeMirrorX": "0", "XYStage-TransposeMirrorY": "0", "PRIME-ReadoutRate": "100MHz 16bit", "XYStage-Name": "Stage", "640 AO-SupportsTriggering": "0", "640-Name": "DA Shutter", "640 Shutter-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "640 AO-Block voltage": "false", "445 Shutter-Sequenceable": "1", "470 AO-MaxVolts": "5.0000", "COM4-BaudRate": "9600", "470-Description": "DA device that is used as a shutter", "520 Shutter-Description": "NI DAC", "PRIME-CCDTemperature": "-14.9200", "Time": "2019-12-17 03:59:58 -0500", "640 AO-TriggerSequenceLength": "1000", "470 AO-Name": "AnalogIO", "XLIGHT Disk slider-Label": "Disk Pos 40um", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "Binning": "1x1", "TE2000-ModelType": "1", "COM3-AnswerTimeout": "500.0000", "555 Shutter-SupportsTriggering": "0", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "TE2000-Port": "COM4", "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Description": "NI DAC", "COM4-AnswerTimeout": "500.0000", "COM1-DelayBetweenCharsMs": "0.0000", "FilterBlock-Label": "Confocal", "445 AO-Name": "AnalogIO", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-Timing-ReadoutTimeNs": "24672000.0000", "528 AO-Demo": "No", "520 AO-Sequenceable": "1", "555-Description": "DA device that is used as a shutter", "405-DA Device": "405 Shutter", "405 AO-TriggerSequenceLength": "1000", "640 AO-MinVolts": "0.0000", "640 Shutter-Block voltage": "false", "FrameIndex": 0, "PRIME-Port": "CMOS", "555 AO-MinVolts": "0.0000", "COM3-Description": "Serial port driver (boost:asio)", "528 AO-Description": "NI DAC", "555 AO-IOChannel": "Dev1/ao6", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "555 AO-TriggerInputLine": "/Dev1/PFI0", "PRIME-ActualInterval-ms": "84.6200", "LudlController-Port": "COM9", "445 Shutter-Demo": "No", "PRIME-Trigger-Expose Out-Mux": "1", "SliceIndex": 8, "PRIME-CCDTemperatureSetPoint": "-15.0000", "405 Shutter-MinVolts": "0.0000", "PRIME-ExposeOutMode": "First Row", "AxisPositions": null, "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Volts": "0.0000", "528-Name": "DA Shutter", "555-State": "0", "XLIGHT Dichroic-Label": "Di03-R635-t1", "445 AO-Sequenceable": "1", "405-Name": "DA Shutter", "405 AO-IOChannel": "Dev1/ao0", "Core-Camera": "PRIME", "Focus-Name": "Focus", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "405 Shutter-IOChannel": "Dev1/ao8", "640 AO-Demo": "No", "640 Shutter-SupportsTriggering": "0", "445 AO-Demo": "No", "555-DA Device": "555 Shutter", "470 Shutter-Description": "NI DAC", "528 AO-Volts": "0.0000", "COM1-DataBits": "8", "Vincent-D3-Address": "x", "520 AO-SupportsTriggering": "0", "405-Description": "DA device that is used as a shutter", "640 Shutter-MinVolts": "0.0000", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "405 AO-Name": "AnalogIO", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "640 AO-TriggerInputLine": "/Dev1/PFI0", "405 AO-TriggerInputLine": "/Dev1/PFI0", "640-Description": "DA device that is used as a shutter", "445 Shutter-TriggerSampleRate": "100000", "470 Shutter-TriggerSequenceLength": "1000", "COM1-StopBits": "1", "520 Shutter-SupportsTriggering": "0", "405-State": "0", "Core-Initialize": "1", "XLIGHT Emission Wheel-State": "3", "520 AO-Description": "NI DAC", "528 AO-TriggerInputLine": "/Dev1/PFI0", "445 Shutter-MaxVolts": "5.0000", "COM9-Handshaking": "Off", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "XLIGHT Spin motor-Label": "On (spinning)", "PRIME-PP 5 ENABLED": "No", "UUID": "926dfd41-2bdf-4c67-ae01-172dc4d089e2", "PRIME-TriggerMode": "Internal Trigger", "COM1-Handshaking": "Off", "PRIME-Timing-PostTriggerDelayNs": "49344000.0000", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "Focus-Position": "16732.9000", "528 Shutter-Block voltage": "false", "Channel": "Il-C", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "555 Shutter-Demo": "No", "405 Shutter-Demo": "No", "528 AO-Name": "AnalogIO", "520-State": "0", "Core-Galvo": "", "405 Shutter-TriggerSampleRate": "100000", "Focus-FocusStepSize_(nm)": "100", "PRIME-PixelType": "16bit", "COM4-DataBits": "8", "470 AO-IOChannel": "Dev1/ao2", "520 AO-MinVolts": "0.0000", "COM9-Parity": "None", "PRIME-CircularBufferEnabled": "ON", "PRIME-PP 6 Reset": "No", "640 AO-Description": "NI DAC", "Source": "PRIME", "445 AO-MinVolts": "0.0000", "PIEZO-Name": "Stage", "445 AO-Description": "NI DAC", "PRIME-CircularBufferFrameCount": "46", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "520 AO-Volts": "0.0000", "528 Shutter-TriggerSampleRate": "100000", "XPositionUm": 23269.100000000002, "OpticalPath-State": "4", "PRIME-PP 3 THRESHOLD": "80", "PRIME-PVCAM Version": "3.7.1", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Gain": "1-HDR", "640 Shutter-TriggerSequenceLength": "1000", "528 AO-SupportsTriggering": "0", "445 AO-TriggerInputLine": "/Dev1/PFI0", "OpticalPath-Name": "OpticalPath", "PRIME-PP 4 ENABLED": "Yes", "PRIME-PVCAM Adapter Version": "1.3.29", "COM3-Name": "COM3", "405 AO-MaxVolts": "5.0000", "520 Shutter-Block voltage": "false", "PRIME-Timing-ClearingTimeNs": "49344000.0000", "Core-Focus": "PIEZO", "528 AO-Block voltage": "false", "TE2000-Description": "Nikon TE2000 hub", "528 AO-MinVolts": "0.0000", "COM4-Parity": "None", "Summary": { "Slices": 11, "ChContrastMin": [ 0, 0, 0, 0, 0, 0 ], "PixelAspect": 1, "SlicesFirst": true, "Interval_ms": 0, "Time": "2019-12-17 03:59:33 -0500", "Prefix": "seq_7", "InitialPositionList": [ { "Label": "Pos0", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [149.91], "Focus": [16657.7], "XYStage": [ 71629.7, 19152.9 ] }, "GridColumnIndex": 0 }, { "Label": "Pos1", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [190.82], "Focus": [16485.4], "XYStage": [ 65702.3, 19396.600000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos2", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [194.69], "Focus": [16446.8], "XYStage": [ 59880.5, 19007.8 ] }, "GridColumnIndex": 0 }, { "Label": "Pos3", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [145.04], "Focus": [16446.6], "XYStage": [ 53616.9, 18818.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos4", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [169.57500000000002], "Focus": [16446.5], "XYStage": [ 47662.8, 18817.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos5", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [131.805], "Focus": [16446.4], "XYStage": [ 41918.8, 18298.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos6", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [113.145], "Focus": [16446.5], "XYStage": [ 36129.9, 18895.8 ] }, "GridColumnIndex": 0 }, { "Label": "Pos7", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [141.005], "Focus": [16446.6], "XYStage": [ 30299.100000000002, 18895.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos8", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [140.97], "Focus": [16446.5], "XYStage": [ 23952.100000000002, 19156 ] }, "GridColumnIndex": 0 }, { "Label": "Pos9", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [184.57500000000002], "Focus": [16677.9], "XYStage": [ 72866.8, -14809.300000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos10", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [167.95000000000002], "Focus": [16557.9], "XYStage": [ 66515.6, -14371.300000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos11", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [168], "Focus": [16618.9], "XYStage": [ 59724.3, -13964.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos12", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [203.205], "Focus": [16779.6], "XYStage": [ 53862.3, -13494.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos13", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [203.20000000000002], "Focus": [16749], "XYStage": [ 48108.3, -13805.400000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos14", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [203.19], "Focus": [16675.9], "XYStage": [ 41645, -13804.900000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos15", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [203.21], "Focus": [16675.7], "XYStage": [ 35352.6, -13805.6 ] }, "GridColumnIndex": 0 }, { "Label": "Pos16", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [203.225], "Focus": [16675.8], "XYStage": [ 29298, -13447.400000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos17", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [203.215], "Focus": [16675.8], "XYStage": [ 23269.100000000002, -13012.300000000001 ] }, "GridColumnIndex": 0 } ], "z-step_um": 10, "GridRow": 0, "BitDepth": 16, "Depth": 2, "Channels": 6, "Source": "Micro-Manager", "ChColors": [ -16711681, -256, -65281, -1, -1, -16776961 ], "MetadataVersion": 10, "KeepShutterOpenChannels": true, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536, 65536 ], "CustomIntervals_ms": [], "Height": 1200, "MicroManagerVersion": "1.4.23 20180219", "GridColumn": 0, "UUID": "3ad8801b-5699-40d6-813b-4ad840863e38", "PixelType": "GRAY16", "Width": 1200, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\Yan\\20191216-YC113-S11S12-miseq", "Frames": 1, "Comment": "", "UserName": "Nikon", "PixelSize_um": 1.1, "ROI": [ 0, 0, 1200, 1200 ], "Date": "2019-12-17", "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC", "P-DAPI-high" ], "KeepShutterOpenSlices": true, "IJType": 1, "ComputerName": "ZadorConfocal2", "Positions": 18, "PositionIndex": 17, "CameraTimeout": 20000, "TimeFirst": false }, "640 AO-Name": "AnalogIO", "Vincent-D3-Command": "Close" }, "FrameKey-0-3-9": { "COM4-StopBits": "1", "528 AO-MaxVolts": "5.0000", "XLIGHT Excitation Wheel-State": "1", "470 Shutter-Name": "AnalogIO", "Core-Shutter": "640", "555 AO-Demo": "No", "XLIGHT Dichroic-State": "2", "XYStage-StepSize-Y": "0.1000", "ZPositionUm": 243.215, "XYStage-StepSize-X": "0.1000", "PRIME-FirmwareVersion": "20.28", "PRIME-ClearCycles": "2", "520-Name": "DA Shutter", "405 Shutter-Name": "AnalogIO", "PIEZO-StepSize": "0.0050", "PRIME-ClearMode": "Pre-Exposure", "470 AO-MinVolts": "0.0000", "405 AO-SupportsTriggering": "1", "CameraChannelIndex": 0, "XYStage-StartSpeed": "500.0000", "COM9-StopBits": "2", "405 AO-Description": "NI DAC", "COM4-DelayBetweenCharsMs": "0.0000", "445 AO-Block voltage": "false", "520 AO-TriggerInputLine": "/Dev1/PFI0", "555 AO-Description": "NI DAC", "520 AO-TriggerSampleRate": "100000", "COM4-Verbose": "1", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM4-Name": "COM4", "405 Shutter-Volts": "0.0000", "Vincent-D3-Port": "COM1", "PRIME-PP 4 MIN ADU AFFECTED": "200", "470 Shutter-MinVolts": "0.0000", "PRIME-PP 3 ENABLED": "Yes", "470 AO-Description": "NI DAC", "PRIME-Description": "PVCAM API device adapter", "520 Shutter-MaxVolts": "5.0000", "520 Shutter-Volts": "0.0000", "528 AO-Sequenceable": "1", "PRIME-Binning": "1x1", "555 Shutter-MaxVolts": "5.0000", "528 Shutter-TriggerSequenceLength": "1000", "405 Shutter-Block voltage": "false", "XLIGHT Spin motor-State": "1", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "Vincent-D3-Time to open (ms)": "35.0000", "Vincent-D3-Time to close (ms)": "35.0000", "445 AO-TriggerSampleRate": "100000", "640 Shutter-IOChannel": "Dev1/ao14", "555 AO-Sequenceable": "1", "520 AO-Name": "AnalogIO", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "470 AO-Block voltage": "false", "COM1-Verbose": "1", "COM9-AnswerTimeout": "10000.0000", "528 Shutter-Name": "AnalogIO", "PRIME-Trigger Timeout (secs)": "10", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "NextFrame": 0, "470 AO-TriggerSequenceLength": "1000", "PRIME-TransposeXY": "0", "445 AO-TriggerSequenceLength": "1000", "COM1-Name": "COM1", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "COM9-DataBits": "8", "COM1-AnswerTimeout": "500.0000", "Camera": "", "TE2000-Version": "V2.50-V2.50-V2.10", "528 Shutter-Volts": "0.0000", "XLIGHT Hub-Name": "XLIGHT Hub", "PIEZO-Description": "Ludl stage driver adapter", "PositionName": "Pos17", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSampleRate": "100000", "PRIME-FanSpeedSetpoint": "High", "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "405 AO-TriggerSampleRate": "100000", "470 Shutter-Block voltage": "false", "470-State": "0", "COM3-Parity": "None", "405 AO-Volts": "0.0000", "PRIME-TransposeMirrorY": "0", "PRIME-ChipName": "GS144BSI", "470 AO-Volts": "0.0000", "PRIME-TransposeMirrorX": "0", "445 Shutter-SupportsTriggering": "0", "470 Shutter-SupportsTriggering": "0", "XLIGHT Hub-Description": "XLIGHT hub", "PRIME-PreampOffLimit": "10000", "470 AO-Sequenceable": "1", "528 Shutter-MaxVolts": "5.0000", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "Core-XYStage": "XYStage", "470 AO-TriggerSampleRate": "100000", "PRIME-Timing-PreTriggerDelayNs": "24672000.0000", "528-DA Device": "528 Shutter", "Focus-Description": "Nikon TE2000 focus adapter", "FilterBlock-State": "5", "520 AO-Demo": "No", "COM3-DelayBetweenCharsMs": "0.0000", "470 Shutter-Volts": "0.0000", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "PRIME-PP 2 THRESHOLD": "125", "PRIME-PP 4 THRESHOLD": "75", "XYStage-Acceleration": "4.0000", "470 Shutter-Demo": "No", "PRIME-Name": "PMPCIECam00", "640-DA Device": "640 Shutter", "405 AO-Block voltage": "false", "ElapsedTime-ms": 742273, "PRIME-Offset": "100", "445 AO-IOChannel": "Dev1/ao1", "640 AO-MaxVolts": "5.0000", "PositionIndex": 17, "PRIME-PP 1 ENABLED": "Yes", "COM1-BaudRate": "9600", "640-State": "1", "COM3-Verbose": "1", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "LudlController-TransmissionDelay": "0", "PRIME-FullWellCapacity": "62000", "PRIME-SMARTStreamingEnabled": "No", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "640 Shutter-Description": "NI DAC", "528-Description": "DA device that is used as a shutter", "COM4-Description": "Serial port driver (boost:asio)", "405 Shutter-Description": "NI DAC", "TE2000-Name": "TE2000", "528 Shutter-Description": "NI DAC", "COM9-Description": "Serial port driver (boost:asio)", "LudlController-Name": "LudlController", "445 Shutter-TriggerSequenceLength": "1000", "PRIME-SerialNumber": "A18B203008", "445 AO-Volts": "0.0000", "PRIME-X-dimension": "1200", "PRIME-ShutterCloseDelay": "0", "XLIGHT Disk slider-State": "2", "Position": "Default", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 AO-IOChannel": "Dev1/ao5", "520 AO-IOChannel": "Dev1/ao4", "528 AO-TriggerSequenceLength": "1000", "405 Shutter-MaxVolts": "5.0000", "470-DA Device": "470 Shutter", "PRIME-FTCapable": "No", "Core-ChannelGroup": "Channels", "555 AO-SupportsTriggering": "0", "PRIME-Y-dimension": "1200", "Frame": 0, "555 Shutter-Sequenceable": "1", "PRIME-Timing-ExposureTimeNs": "499998000.0000", "Core-ImageProcessor": "", "528-State": "0", "520 AO-Block voltage": "false", "XLIGHT Excitation Wheel-Label": "QUAD", "640 Shutter-Demo": "No", "COM3-StopBits": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "520 Shutter-IOChannel": "Dev1/ao11", "Width": 1200, "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-SupportsTriggering": "0", "640 AO-TriggerSampleRate": "100000", "520-Description": "DA device that is used as a shutter", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "405 Shutter-Sequenceable": "1", "405 Shutter-SupportsTriggering": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-TriggerSampleRate": "100000", "640 Shutter-MaxVolts": "5.0000", "555 AO-MaxVolts": "5.0000", "520 AO-TriggerSequenceLength": "1000", "COM3-BaudRate": "9600", "COM9-BaudRate": "9600", "Vincent-D3-Channel #": "Ch. #1", "PRIME-PMode": "Normal", "OpticalPath-Label": "Left(100)", "BitDepth": 16, "555 AO-TriggerSequenceLength": "1000", "445 Shutter-Description": "NI DAC", "405 AO-Sequenceable": "1", "PRIME-AcquisitionMethod": "Callbacks", "555 AO-Name": "AnalogIO", "PRIME-ShutterMode": "Pre-Exposure", "COM3-Handshaking": "Off", "445 AO-SupportsTriggering": "0", "ROI": "0-0-1200-1200", "COM9-Verbose": "1", "PRIME-Color": "OFF", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "PRIME-ShutterOpenDelay": "0", "COM1-Parity": "None", "Slice": 9, "SlicePosition": 40, "COM9-DelayBetweenCharsMs": "0.0000", "555-Name": "DA Shutter", "PIEZO-LudlSingleAxisName": "B", "XLIGHT Emission Wheel-Label": "725/40 (C)", "640 Shutter-Name": "AnalogIO", "640 AO-Sequenceable": "1", "520 Shutter-Demo": "No", "528 Shutter-Demo": "No", "470-Name": "DA Shutter", "520-DA Device": "520 Shutter", "XYStage-Description": "Ludl XY stage driver adapter", "445 Shutter-MinVolts": "0.0000", "528 AO-TriggerSampleRate": "100000", "405 AO-MinVolts": "0.0000", "405 Shutter-TriggerSequenceLength": "1000", "Height": 1200, "555 AO-Block voltage": "false", "640 Shutter-Volts": "5.0000", "PixelType": "GRAY16", "445 Shutter-Block voltage": "false", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "520 Shutter-TriggerSequenceLength": "1000", "COM9-Name": "COM9", "Core-SLM": "", "555 AO-Volts": "0.0000", "555 Shutter-Block voltage": "false", "XYStage-Speed": "1800.0000", "520 AO-MaxVolts": "5.0000", "528 Shutter-SupportsTriggering": "0", "COM1-Description": "Serial port driver (boost:asio)", "PRIME-MetadataEnabled": "No", "520 Shutter-MinVolts": "0.0000", "470 Shutter-Sequenceable": "1", "PRIME-PreampDelay": "0", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-MinVolts": "0.0000", "ChannelIndex": 3, "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Core-AutoShutter": "1", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "Exposure-ms": 520, "PRIME-PP 2 ENABLED": "Yes", "640 Shutter-TriggerSampleRate": "100000", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "470 Shutter-IOChannel": "Dev1/ao10", "405 AO-Demo": "No", "PixelSizeUm": 1.1, "470 AO-Demo": "No", "470 Shutter-MaxVolts": "5.0000", "Vincent-D3-Name": "Vincent-D3", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FileName": "img_000000000_Il-C_009.tif", "LudlController-Reset": "Operate", "PRIME-Exposure": "520.00", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "445-Name": "DA Shutter", "555 Shutter-Name": "AnalogIO", "520 Shutter-TriggerSampleRate": "100000", "445 Shutter-IOChannel": "Dev1/ao9", "PRIME-TransposeCorrection": "0", "XYStage-StepSize": "0.1000", "445-Description": "DA device that is used as a shutter", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 AO-IOChannel": "Dev1/ao7", "520 Shutter-Name": "AnalogIO", "COM3-DataBits": "8", "640 AO-Volts": "4.5000", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "555 Shutter-IOChannel": "Dev1/ao13", "WaitInterval": null, "520 Shutter-Sequenceable": "1", "YPositionUm": -13012.300000000001, "445-State": "0", "445-DA Device": "445 Shutter", "PRIME-CircularBufferAutoSize": "ON", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "555 AO-TriggerSampleRate": "100000", "445 Shutter-Volts": "0.0000", "XLIGHT Hub-Port": "COM3", "Core-AutoFocus": "", "COM4-Handshaking": "Off", "528 Shutter-MinVolts": "0.0000", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "445 Shutter-Name": "AnalogIO", "528 Shutter-Sequenceable": "1", "XYStage-TransposeMirrorX": "0", "XYStage-TransposeMirrorY": "0", "PRIME-ReadoutRate": "100MHz 16bit", "XYStage-Name": "Stage", "640 AO-SupportsTriggering": "0", "640-Name": "DA Shutter", "640 Shutter-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "640 AO-Block voltage": "false", "445 Shutter-Sequenceable": "1", "470 AO-MaxVolts": "5.0000", "COM4-BaudRate": "9600", "470-Description": "DA device that is used as a shutter", "520 Shutter-Description": "NI DAC", "PRIME-CCDTemperature": "-14.9200", "Time": "2019-12-17 03:59:59 -0500", "640 AO-TriggerSequenceLength": "1000", "470 AO-Name": "AnalogIO", "XLIGHT Disk slider-Label": "Disk Pos 40um", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "Binning": "1x1", "TE2000-ModelType": "1", "COM3-AnswerTimeout": "500.0000", "555 Shutter-SupportsTriggering": "0", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "TE2000-Port": "COM4", "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Description": "NI DAC", "COM4-AnswerTimeout": "500.0000", "COM1-DelayBetweenCharsMs": "0.0000", "FilterBlock-Label": "Confocal", "445 AO-Name": "AnalogIO", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-Timing-ReadoutTimeNs": "24672000.0000", "528 AO-Demo": "No", "520 AO-Sequenceable": "1", "555-Description": "DA device that is used as a shutter", "405-DA Device": "405 Shutter", "405 AO-TriggerSequenceLength": "1000", "640 AO-MinVolts": "0.0000", "640 Shutter-Block voltage": "false", "FrameIndex": 0, "PRIME-Port": "CMOS", "555 AO-MinVolts": "0.0000", "COM3-Description": "Serial port driver (boost:asio)", "528 AO-Description": "NI DAC", "555 AO-IOChannel": "Dev1/ao6", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "555 AO-TriggerInputLine": "/Dev1/PFI0", "PRIME-ActualInterval-ms": "84.6200", "LudlController-Port": "COM9", "445 Shutter-Demo": "No", "PRIME-Trigger-Expose Out-Mux": "1", "SliceIndex": 9, "PRIME-CCDTemperatureSetPoint": "-15.0000", "405 Shutter-MinVolts": "0.0000", "PRIME-ExposeOutMode": "First Row", "AxisPositions": null, "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Volts": "0.0000", "528-Name": "DA Shutter", "555-State": "0", "XLIGHT Dichroic-Label": "Di03-R635-t1", "445 AO-Sequenceable": "1", "405-Name": "DA Shutter", "405 AO-IOChannel": "Dev1/ao0", "Core-Camera": "PRIME", "Focus-Name": "Focus", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "405 Shutter-IOChannel": "Dev1/ao8", "640 AO-Demo": "No", "640 Shutter-SupportsTriggering": "0", "445 AO-Demo": "No", "555-DA Device": "555 Shutter", "470 Shutter-Description": "NI DAC", "528 AO-Volts": "0.0000", "COM1-DataBits": "8", "Vincent-D3-Address": "x", "520 AO-SupportsTriggering": "0", "405-Description": "DA device that is used as a shutter", "640 Shutter-MinVolts": "0.0000", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "405 AO-Name": "AnalogIO", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "640 AO-TriggerInputLine": "/Dev1/PFI0", "405 AO-TriggerInputLine": "/Dev1/PFI0", "640-Description": "DA device that is used as a shutter", "445 Shutter-TriggerSampleRate": "100000", "470 Shutter-TriggerSequenceLength": "1000", "COM1-StopBits": "1", "520 Shutter-SupportsTriggering": "0", "405-State": "0", "Core-Initialize": "1", "XLIGHT Emission Wheel-State": "3", "520 AO-Description": "NI DAC", "528 AO-TriggerInputLine": "/Dev1/PFI0", "445 Shutter-MaxVolts": "5.0000", "COM9-Handshaking": "Off", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "XLIGHT Spin motor-Label": "On (spinning)", "PRIME-PP 5 ENABLED": "No", "UUID": "642eab57-c830-474f-ad48-9f2c0f5dcca4", "PRIME-TriggerMode": "Internal Trigger", "COM1-Handshaking": "Off", "PRIME-Timing-PostTriggerDelayNs": "49344000.0000", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "Focus-Position": "16732.9000", "528 Shutter-Block voltage": "false", "Channel": "Il-C", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "555 Shutter-Demo": "No", "405 Shutter-Demo": "No", "528 AO-Name": "AnalogIO", "520-State": "0", "Core-Galvo": "", "405 Shutter-TriggerSampleRate": "100000", "Focus-FocusStepSize_(nm)": "100", "PRIME-PixelType": "16bit", "COM4-DataBits": "8", "470 AO-IOChannel": "Dev1/ao2", "520 AO-MinVolts": "0.0000", "COM9-Parity": "None", "PRIME-CircularBufferEnabled": "ON", "PRIME-PP 6 Reset": "No", "640 AO-Description": "NI DAC", "Source": "PRIME", "445 AO-MinVolts": "0.0000", "PIEZO-Name": "Stage", "445 AO-Description": "NI DAC", "PRIME-CircularBufferFrameCount": "46", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "520 AO-Volts": "0.0000", "528 Shutter-TriggerSampleRate": "100000", "XPositionUm": 23269.100000000002, "OpticalPath-State": "4", "PRIME-PP 3 THRESHOLD": "80", "PRIME-PVCAM Version": "3.7.1", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Gain": "1-HDR", "640 Shutter-TriggerSequenceLength": "1000", "528 AO-SupportsTriggering": "0", "445 AO-TriggerInputLine": "/Dev1/PFI0", "OpticalPath-Name": "OpticalPath", "PRIME-PP 4 ENABLED": "Yes", "PRIME-PVCAM Adapter Version": "1.3.29", "COM3-Name": "COM3", "405 AO-MaxVolts": "5.0000", "520 Shutter-Block voltage": "false", "PRIME-Timing-ClearingTimeNs": "49344000.0000", "Core-Focus": "PIEZO", "528 AO-Block voltage": "false", "TE2000-Description": "Nikon TE2000 hub", "528 AO-MinVolts": "0.0000", "COM4-Parity": "None", "Summary": { "Slices": 11, "ChContrastMin": [ 0, 0, 0, 0, 0, 0 ], "PixelAspect": 1, "SlicesFirst": true, "Interval_ms": 0, "Time": "2019-12-17 03:59:33 -0500", "Prefix": "seq_7", "InitialPositionList": [ { "Label": "Pos0", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [149.91], "Focus": [16657.7], "XYStage": [ 71629.7, 19152.9 ] }, "GridColumnIndex": 0 }, { "Label": "Pos1", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [190.82], "Focus": [16485.4], "XYStage": [ 65702.3, 19396.600000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos2", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [194.69], "Focus": [16446.8], "XYStage": [ 59880.5, 19007.8 ] }, "GridColumnIndex": 0 }, { "Label": "Pos3", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [145.04], "Focus": [16446.6], "XYStage": [ 53616.9, 18818.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos4", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [169.57500000000002], "Focus": [16446.5], "XYStage": [ 47662.8, 18817.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos5", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [131.805], "Focus": [16446.4], "XYStage": [ 41918.8, 18298.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos6", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [113.145], "Focus": [16446.5], "XYStage": [ 36129.9, 18895.8 ] }, "GridColumnIndex": 0 }, { "Label": "Pos7", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [141.005], "Focus": [16446.6], "XYStage": [ 30299.100000000002, 18895.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos8", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [140.97], "Focus": [16446.5], "XYStage": [ 23952.100000000002, 19156 ] }, "GridColumnIndex": 0 }, { "Label": "Pos9", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [184.57500000000002], "Focus": [16677.9], "XYStage": [ 72866.8, -14809.300000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos10", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [167.95000000000002], "Focus": [16557.9], "XYStage": [ 66515.6, -14371.300000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos11", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [168], "Focus": [16618.9], "XYStage": [ 59724.3, -13964.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos12", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [203.205], "Focus": [16779.6], "XYStage": [ 53862.3, -13494.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos13", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [203.20000000000002], "Focus": [16749], "XYStage": [ 48108.3, -13805.400000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos14", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [203.19], "Focus": [16675.9], "XYStage": [ 41645, -13804.900000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos15", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [203.21], "Focus": [16675.7], "XYStage": [ 35352.6, -13805.6 ] }, "GridColumnIndex": 0 }, { "Label": "Pos16", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [203.225], "Focus": [16675.8], "XYStage": [ 29298, -13447.400000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos17", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [203.215], "Focus": [16675.8], "XYStage": [ 23269.100000000002, -13012.300000000001 ] }, "GridColumnIndex": 0 } ], "z-step_um": 10, "GridRow": 0, "BitDepth": 16, "Depth": 2, "Channels": 6, "Source": "Micro-Manager", "ChColors": [ -16711681, -256, -65281, -1, -1, -16776961 ], "MetadataVersion": 10, "KeepShutterOpenChannels": true, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536, 65536 ], "CustomIntervals_ms": [], "Height": 1200, "MicroManagerVersion": "1.4.23 20180219", "GridColumn": 0, "UUID": "3ad8801b-5699-40d6-813b-4ad840863e38", "PixelType": "GRAY16", "Width": 1200, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\Yan\\20191216-YC113-S11S12-miseq", "Frames": 1, "Comment": "", "UserName": "Nikon", "PixelSize_um": 1.1, "ROI": [ 0, 0, 1200, 1200 ], "Date": "2019-12-17", "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC", "P-DAPI-high" ], "KeepShutterOpenSlices": true, "IJType": 1, "ComputerName": "ZadorConfocal2", "Positions": 18, "PositionIndex": 17, "CameraTimeout": 20000, "TimeFirst": false }, "640 AO-Name": "AnalogIO", "Vincent-D3-Command": "Close" }, "FrameKey-0-3-10": { "COM4-StopBits": "1", "528 AO-MaxVolts": "5.0000", "XLIGHT Excitation Wheel-State": "1", "470 Shutter-Name": "AnalogIO", "Core-Shutter": "640", "555 AO-Demo": "No", "XLIGHT Dichroic-State": "2", "XYStage-StepSize-Y": "0.1000", "ZPositionUm": 253.215, "XYStage-StepSize-X": "0.1000", "PRIME-FirmwareVersion": "20.28", "PRIME-ClearCycles": "2", "520-Name": "DA Shutter", "405 Shutter-Name": "AnalogIO", "PIEZO-StepSize": "0.0050", "PRIME-ClearMode": "Pre-Exposure", "470 AO-MinVolts": "0.0000", "405 AO-SupportsTriggering": "1", "CameraChannelIndex": 0, "XYStage-StartSpeed": "500.0000", "COM9-StopBits": "2", "405 AO-Description": "NI DAC", "COM4-DelayBetweenCharsMs": "0.0000", "445 AO-Block voltage": "false", "520 AO-TriggerInputLine": "/Dev1/PFI0", "555 AO-Description": "NI DAC", "520 AO-TriggerSampleRate": "100000", "COM4-Verbose": "1", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM4-Name": "COM4", "405 Shutter-Volts": "0.0000", "Vincent-D3-Port": "COM1", "PRIME-PP 4 MIN ADU AFFECTED": "200", "470 Shutter-MinVolts": "0.0000", "PRIME-PP 3 ENABLED": "Yes", "470 AO-Description": "NI DAC", "PRIME-Description": "PVCAM API device adapter", "520 Shutter-MaxVolts": "5.0000", "520 Shutter-Volts": "0.0000", "528 AO-Sequenceable": "1", "PRIME-Binning": "1x1", "555 Shutter-MaxVolts": "5.0000", "528 Shutter-TriggerSequenceLength": "1000", "405 Shutter-Block voltage": "false", "XLIGHT Spin motor-State": "1", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "Vincent-D3-Time to open (ms)": "35.0000", "Vincent-D3-Time to close (ms)": "35.0000", "445 AO-TriggerSampleRate": "100000", "640 Shutter-IOChannel": "Dev1/ao14", "555 AO-Sequenceable": "1", "520 AO-Name": "AnalogIO", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "470 AO-Block voltage": "false", "COM1-Verbose": "1", "COM9-AnswerTimeout": "10000.0000", "528 Shutter-Name": "AnalogIO", "PRIME-Trigger Timeout (secs)": "10", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "NextFrame": 0, "470 AO-TriggerSequenceLength": "1000", "PRIME-TransposeXY": "0", "445 AO-TriggerSequenceLength": "1000", "COM1-Name": "COM1", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "COM9-DataBits": "8", "COM1-AnswerTimeout": "500.0000", "Camera": "", "TE2000-Version": "V2.50-V2.50-V2.10", "528 Shutter-Volts": "0.0000", "XLIGHT Hub-Name": "XLIGHT Hub", "PIEZO-Description": "Ludl stage driver adapter", "PositionName": "Pos17", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSampleRate": "100000", "PRIME-FanSpeedSetpoint": "High", "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "405 AO-TriggerSampleRate": "100000", "470 Shutter-Block voltage": "false", "470-State": "0", "COM3-Parity": "None", "405 AO-Volts": "0.0000", "PRIME-TransposeMirrorY": "0", "PRIME-ChipName": "GS144BSI", "470 AO-Volts": "0.0000", "PRIME-TransposeMirrorX": "0", "445 Shutter-SupportsTriggering": "0", "470 Shutter-SupportsTriggering": "0", "XLIGHT Hub-Description": "XLIGHT hub", "PRIME-PreampOffLimit": "10000", "470 AO-Sequenceable": "1", "528 Shutter-MaxVolts": "5.0000", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "Core-XYStage": "XYStage", "470 AO-TriggerSampleRate": "100000", "PRIME-Timing-PreTriggerDelayNs": "24672000.0000", "528-DA Device": "528 Shutter", "Focus-Description": "Nikon TE2000 focus adapter", "FilterBlock-State": "5", "520 AO-Demo": "No", "COM3-DelayBetweenCharsMs": "0.0000", "470 Shutter-Volts": "0.0000", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "PRIME-PP 2 THRESHOLD": "125", "PRIME-PP 4 THRESHOLD": "75", "XYStage-Acceleration": "4.0000", "470 Shutter-Demo": "No", "PRIME-Name": "PMPCIECam00", "640-DA Device": "640 Shutter", "405 AO-Block voltage": "false", "ElapsedTime-ms": 743031, "PRIME-Offset": "100", "445 AO-IOChannel": "Dev1/ao1", "640 AO-MaxVolts": "5.0000", "PositionIndex": 17, "PRIME-PP 1 ENABLED": "Yes", "COM1-BaudRate": "9600", "640-State": "0", "COM3-Verbose": "1", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "LudlController-TransmissionDelay": "0", "PRIME-FullWellCapacity": "62000", "PRIME-SMARTStreamingEnabled": "No", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "640 Shutter-Description": "NI DAC", "528-Description": "DA device that is used as a shutter", "COM4-Description": "Serial port driver (boost:asio)", "405 Shutter-Description": "NI DAC", "TE2000-Name": "TE2000", "528 Shutter-Description": "NI DAC", "COM9-Description": "Serial port driver (boost:asio)", "LudlController-Name": "LudlController", "445 Shutter-TriggerSequenceLength": "1000", "PRIME-SerialNumber": "A18B203008", "445 AO-Volts": "0.0000", "PRIME-X-dimension": "1200", "PRIME-ShutterCloseDelay": "0", "XLIGHT Disk slider-State": "2", "Position": "Default", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 AO-IOChannel": "Dev1/ao5", "520 AO-IOChannel": "Dev1/ao4", "528 AO-TriggerSequenceLength": "1000", "405 Shutter-MaxVolts": "5.0000", "470-DA Device": "470 Shutter", "PRIME-FTCapable": "No", "Core-ChannelGroup": "Channels", "555 AO-SupportsTriggering": "0", "PRIME-Y-dimension": "1200", "Frame": 0, "555 Shutter-Sequenceable": "1", "PRIME-Timing-ExposureTimeNs": "499998000.0000", "Core-ImageProcessor": "", "528-State": "0", "520 AO-Block voltage": "false", "XLIGHT Excitation Wheel-Label": "QUAD", "640 Shutter-Demo": "No", "COM3-StopBits": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "520 Shutter-IOChannel": "Dev1/ao11", "Width": 1200, "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-SupportsTriggering": "0", "640 AO-TriggerSampleRate": "100000", "520-Description": "DA device that is used as a shutter", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "405 Shutter-Sequenceable": "1", "405 Shutter-SupportsTriggering": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-TriggerSampleRate": "100000", "640 Shutter-MaxVolts": "5.0000", "555 AO-MaxVolts": "5.0000", "520 AO-TriggerSequenceLength": "1000", "COM3-BaudRate": "9600", "COM9-BaudRate": "9600", "Vincent-D3-Channel #": "Ch. #1", "PRIME-PMode": "Normal", "OpticalPath-Label": "Left(100)", "BitDepth": 16, "555 AO-TriggerSequenceLength": "1000", "445 Shutter-Description": "NI DAC", "405 AO-Sequenceable": "1", "PRIME-AcquisitionMethod": "Callbacks", "555 AO-Name": "AnalogIO", "PRIME-ShutterMode": "Pre-Exposure", "COM3-Handshaking": "Off", "445 AO-SupportsTriggering": "0", "ROI": "0-0-1200-1200", "COM9-Verbose": "1", "PRIME-Color": "OFF", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "PRIME-ShutterOpenDelay": "0", "COM1-Parity": "None", "Slice": 10, "SlicePosition": 50, "COM9-DelayBetweenCharsMs": "0.0000", "555-Name": "DA Shutter", "PIEZO-LudlSingleAxisName": "B", "XLIGHT Emission Wheel-Label": "725/40 (C)", "640 Shutter-Name": "AnalogIO", "640 AO-Sequenceable": "1", "520 Shutter-Demo": "No", "528 Shutter-Demo": "No", "470-Name": "DA Shutter", "520-DA Device": "520 Shutter", "XYStage-Description": "Ludl XY stage driver adapter", "445 Shutter-MinVolts": "0.0000", "528 AO-TriggerSampleRate": "100000", "405 AO-MinVolts": "0.0000", "405 Shutter-TriggerSequenceLength": "1000", "Height": 1200, "555 AO-Block voltage": "false", "640 Shutter-Volts": "5.0000", "PixelType": "GRAY16", "445 Shutter-Block voltage": "false", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "520 Shutter-TriggerSequenceLength": "1000", "COM9-Name": "COM9", "Core-SLM": "", "555 AO-Volts": "0.0000", "555 Shutter-Block voltage": "false", "XYStage-Speed": "1800.0000", "520 AO-MaxVolts": "5.0000", "528 Shutter-SupportsTriggering": "0", "COM1-Description": "Serial port driver (boost:asio)", "PRIME-MetadataEnabled": "No", "520 Shutter-MinVolts": "0.0000", "470 Shutter-Sequenceable": "1", "PRIME-PreampDelay": "0", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-MinVolts": "0.0000", "ChannelIndex": 3, "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Core-AutoShutter": "1", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "Exposure-ms": 520, "PRIME-PP 2 ENABLED": "Yes", "640 Shutter-TriggerSampleRate": "100000", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "470 Shutter-IOChannel": "Dev1/ao10", "405 AO-Demo": "No", "PixelSizeUm": 1.1, "470 AO-Demo": "No", "470 Shutter-MaxVolts": "5.0000", "Vincent-D3-Name": "Vincent-D3", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FileName": "img_000000000_Il-C_010.tif", "LudlController-Reset": "Operate", "PRIME-Exposure": "520.00", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "445-Name": "DA Shutter", "555 Shutter-Name": "AnalogIO", "520 Shutter-TriggerSampleRate": "100000", "445 Shutter-IOChannel": "Dev1/ao9", "PRIME-TransposeCorrection": "0", "XYStage-StepSize": "0.1000", "445-Description": "DA device that is used as a shutter", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 AO-IOChannel": "Dev1/ao7", "520 Shutter-Name": "AnalogIO", "COM3-DataBits": "8", "640 AO-Volts": "4.5000", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "555 Shutter-IOChannel": "Dev1/ao13", "WaitInterval": null, "520 Shutter-Sequenceable": "1", "YPositionUm": -13012.300000000001, "445-State": "0", "445-DA Device": "445 Shutter", "PRIME-CircularBufferAutoSize": "ON", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "555 AO-TriggerSampleRate": "100000", "445 Shutter-Volts": "0.0000", "XLIGHT Hub-Port": "COM3", "Core-AutoFocus": "", "COM4-Handshaking": "Off", "528 Shutter-MinVolts": "0.0000", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "445 Shutter-Name": "AnalogIO", "528 Shutter-Sequenceable": "1", "XYStage-TransposeMirrorX": "0", "XYStage-TransposeMirrorY": "0", "PRIME-ReadoutRate": "100MHz 16bit", "XYStage-Name": "Stage", "640 AO-SupportsTriggering": "0", "640-Name": "DA Shutter", "640 Shutter-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "640 AO-Block voltage": "false", "445 Shutter-Sequenceable": "1", "470 AO-MaxVolts": "5.0000", "COM4-BaudRate": "9600", "470-Description": "DA device that is used as a shutter", "520 Shutter-Description": "NI DAC", "PRIME-CCDTemperature": "-14.9200", "Time": "2019-12-17 04:00:00 -0500", "640 AO-TriggerSequenceLength": "1000", "470 AO-Name": "AnalogIO", "XLIGHT Disk slider-Label": "Disk Pos 40um", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "Binning": "1x1", "TE2000-ModelType": "1", "COM3-AnswerTimeout": "500.0000", "555 Shutter-SupportsTriggering": "0", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "TE2000-Port": "COM4", "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Description": "NI DAC", "COM4-AnswerTimeout": "500.0000", "COM1-DelayBetweenCharsMs": "0.0000", "FilterBlock-Label": "Confocal", "445 AO-Name": "AnalogIO", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-Timing-ReadoutTimeNs": "24672000.0000", "528 AO-Demo": "No", "520 AO-Sequenceable": "1", "555-Description": "DA device that is used as a shutter", "405-DA Device": "405 Shutter", "405 AO-TriggerSequenceLength": "1000", "640 AO-MinVolts": "0.0000", "640 Shutter-Block voltage": "false", "FrameIndex": 0, "PRIME-Port": "CMOS", "555 AO-MinVolts": "0.0000", "COM3-Description": "Serial port driver (boost:asio)", "528 AO-Description": "NI DAC", "555 AO-IOChannel": "Dev1/ao6", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "555 AO-TriggerInputLine": "/Dev1/PFI0", "PRIME-ActualInterval-ms": "84.6200", "LudlController-Port": "COM9", "445 Shutter-Demo": "No", "PRIME-Trigger-Expose Out-Mux": "1", "SliceIndex": 10, "PRIME-CCDTemperatureSetPoint": "-15.0000", "405 Shutter-MinVolts": "0.0000", "PRIME-ExposeOutMode": "First Row", "AxisPositions": null, "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Volts": "0.0000", "528-Name": "DA Shutter", "555-State": "0", "XLIGHT Dichroic-Label": "Di03-R635-t1", "445 AO-Sequenceable": "1", "405-Name": "DA Shutter", "405 AO-IOChannel": "Dev1/ao0", "Core-Camera": "PRIME", "Focus-Name": "Focus", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "405 Shutter-IOChannel": "Dev1/ao8", "640 AO-Demo": "No", "640 Shutter-SupportsTriggering": "0", "445 AO-Demo": "No", "555-DA Device": "555 Shutter", "470 Shutter-Description": "NI DAC", "528 AO-Volts": "0.0000", "COM1-DataBits": "8", "Vincent-D3-Address": "x", "520 AO-SupportsTriggering": "0", "405-Description": "DA device that is used as a shutter", "640 Shutter-MinVolts": "0.0000", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "405 AO-Name": "AnalogIO", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "640 AO-TriggerInputLine": "/Dev1/PFI0", "405 AO-TriggerInputLine": "/Dev1/PFI0", "640-Description": "DA device that is used as a shutter", "445 Shutter-TriggerSampleRate": "100000", "470 Shutter-TriggerSequenceLength": "1000", "COM1-StopBits": "1", "520 Shutter-SupportsTriggering": "0", "405-State": "0", "Core-Initialize": "1", "XLIGHT Emission Wheel-State": "3", "520 AO-Description": "NI DAC", "528 AO-TriggerInputLine": "/Dev1/PFI0", "445 Shutter-MaxVolts": "5.0000", "COM9-Handshaking": "Off", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "XLIGHT Spin motor-Label": "On (spinning)", "PRIME-PP 5 ENABLED": "No", "UUID": "8249af22-5859-4bfe-b34f-6eff83f14683", "PRIME-TriggerMode": "Internal Trigger", "COM1-Handshaking": "Off", "PRIME-Timing-PostTriggerDelayNs": "49344000.0000", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "Focus-Position": "16732.9000", "528 Shutter-Block voltage": "false", "Channel": "Il-C", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "555 Shutter-Demo": "No", "405 Shutter-Demo": "No", "528 AO-Name": "AnalogIO", "520-State": "0", "Core-Galvo": "", "405 Shutter-TriggerSampleRate": "100000", "Focus-FocusStepSize_(nm)": "100", "PRIME-PixelType": "16bit", "COM4-DataBits": "8", "470 AO-IOChannel": "Dev1/ao2", "520 AO-MinVolts": "0.0000", "COM9-Parity": "None", "PRIME-CircularBufferEnabled": "ON", "PRIME-PP 6 Reset": "No", "640 AO-Description": "NI DAC", "Source": "PRIME", "445 AO-MinVolts": "0.0000", "PIEZO-Name": "Stage", "445 AO-Description": "NI DAC", "PRIME-CircularBufferFrameCount": "46", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "520 AO-Volts": "0.0000", "528 Shutter-TriggerSampleRate": "100000", "XPositionUm": 23269.100000000002, "OpticalPath-State": "4", "PRIME-PP 3 THRESHOLD": "80", "PRIME-PVCAM Version": "3.7.1", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Gain": "1-HDR", "640 Shutter-TriggerSequenceLength": "1000", "528 AO-SupportsTriggering": "0", "445 AO-TriggerInputLine": "/Dev1/PFI0", "OpticalPath-Name": "OpticalPath", "PRIME-PP 4 ENABLED": "Yes", "PRIME-PVCAM Adapter Version": "1.3.29", "COM3-Name": "COM3", "405 AO-MaxVolts": "5.0000", "520 Shutter-Block voltage": "false", "PRIME-Timing-ClearingTimeNs": "49344000.0000", "Core-Focus": "PIEZO", "528 AO-Block voltage": "false", "TE2000-Description": "Nikon TE2000 hub", "528 AO-MinVolts": "0.0000", "COM4-Parity": "None", "Summary": { "Slices": 11, "ChContrastMin": [ 0, 0, 0, 0, 0, 0 ], "PixelAspect": 1, "SlicesFirst": true, "Interval_ms": 0, "Time": "2019-12-17 03:59:33 -0500", "Prefix": "seq_7", "InitialPositionList": [ { "Label": "Pos0", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [149.91], "Focus": [16657.7], "XYStage": [ 71629.7, 19152.9 ] }, "GridColumnIndex": 0 }, { "Label": "Pos1", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [190.82], "Focus": [16485.4], "XYStage": [ 65702.3, 19396.600000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos2", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [194.69], "Focus": [16446.8], "XYStage": [ 59880.5, 19007.8 ] }, "GridColumnIndex": 0 }, { "Label": "Pos3", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [145.04], "Focus": [16446.6], "XYStage": [ 53616.9, 18818.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos4", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [169.57500000000002], "Focus": [16446.5], "XYStage": [ 47662.8, 18817.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos5", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [131.805], "Focus": [16446.4], "XYStage": [ 41918.8, 18298.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos6", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [113.145], "Focus": [16446.5], "XYStage": [ 36129.9, 18895.8 ] }, "GridColumnIndex": 0 }, { "Label": "Pos7", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [141.005], "Focus": [16446.6], "XYStage": [ 30299.100000000002, 18895.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos8", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [140.97], "Focus": [16446.5], "XYStage": [ 23952.100000000002, 19156 ] }, "GridColumnIndex": 0 }, { "Label": "Pos9", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [184.57500000000002], "Focus": [16677.9], "XYStage": [ 72866.8, -14809.300000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos10", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [167.95000000000002], "Focus": [16557.9], "XYStage": [ 66515.6, -14371.300000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos11", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [168], "Focus": [16618.9], "XYStage": [ 59724.3, -13964.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos12", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [203.205], "Focus": [16779.6], "XYStage": [ 53862.3, -13494.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos13", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [203.20000000000002], "Focus": [16749], "XYStage": [ 48108.3, -13805.400000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos14", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [203.19], "Focus": [16675.9], "XYStage": [ 41645, -13804.900000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos15", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [203.21], "Focus": [16675.7], "XYStage": [ 35352.6, -13805.6 ] }, "GridColumnIndex": 0 }, { "Label": "Pos16", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [203.225], "Focus": [16675.8], "XYStage": [ 29298, -13447.400000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos17", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [203.215], "Focus": [16675.8], "XYStage": [ 23269.100000000002, -13012.300000000001 ] }, "GridColumnIndex": 0 } ], "z-step_um": 10, "GridRow": 0, "BitDepth": 16, "Depth": 2, "Channels": 6, "Source": "Micro-Manager", "ChColors": [ -16711681, -256, -65281, -1, -1, -16776961 ], "MetadataVersion": 10, "KeepShutterOpenChannels": true, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536, 65536 ], "CustomIntervals_ms": [], "Height": 1200, "MicroManagerVersion": "1.4.23 20180219", "GridColumn": 0, "UUID": "3ad8801b-5699-40d6-813b-4ad840863e38", "PixelType": "GRAY16", "Width": 1200, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\Yan\\20191216-YC113-S11S12-miseq", "Frames": 1, "Comment": "", "UserName": "Nikon", "PixelSize_um": 1.1, "ROI": [ 0, 0, 1200, 1200 ], "Date": "2019-12-17", "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC", "P-DAPI-high" ], "KeepShutterOpenSlices": true, "IJType": 1, "ComputerName": "ZadorConfocal2", "Positions": 18, "PositionIndex": 17, "CameraTimeout": 20000, "TimeFirst": false }, "640 AO-Name": "AnalogIO", "Vincent-D3-Command": "Close" }, "FrameKey-0-4-0": { "COM4-StopBits": "1", "528 AO-MaxVolts": "5.0000", "XLIGHT Excitation Wheel-State": "1", "470 Shutter-Name": "AnalogIO", "Core-Shutter": "Vincent-D3", "555 AO-Demo": "No", "XLIGHT Dichroic-State": "2", "XYStage-StepSize-Y": "0.1000", "ZPositionUm": 153.215, "XYStage-StepSize-X": "0.1000", "PRIME-FirmwareVersion": "20.28", "PRIME-ClearCycles": "2", "520-Name": "DA Shutter", "405 Shutter-Name": "AnalogIO", "PIEZO-StepSize": "0.0050", "PRIME-ClearMode": "Pre-Exposure", "470 AO-MinVolts": "0.0000", "405 AO-SupportsTriggering": "1", "CameraChannelIndex": 0, "XYStage-StartSpeed": "500.0000", "COM9-StopBits": "2", "405 AO-Description": "NI DAC", "COM4-DelayBetweenCharsMs": "0.0000", "445 AO-Block voltage": "false", "520 AO-TriggerInputLine": "/Dev1/PFI0", "555 AO-Description": "NI DAC", "520 AO-TriggerSampleRate": "100000", "COM4-Verbose": "1", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM4-Name": "COM4", "405 Shutter-Volts": "0.0000", "Vincent-D3-Port": "COM1", "PRIME-PP 4 MIN ADU AFFECTED": "200", "470 Shutter-MinVolts": "0.0000", "PRIME-PP 3 ENABLED": "Yes", "470 AO-Description": "NI DAC", "PRIME-Description": "PVCAM API device adapter", "520 Shutter-MaxVolts": "5.0000", "520 Shutter-Volts": "0.0000", "528 AO-Sequenceable": "1", "PRIME-Binning": "1x1", "555 Shutter-MaxVolts": "5.0000", "528 Shutter-TriggerSequenceLength": "1000", "405 Shutter-Block voltage": "false", "XLIGHT Spin motor-State": "1", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "Vincent-D3-Time to open (ms)": "35.0000", "Vincent-D3-Time to close (ms)": "35.0000", "445 AO-TriggerSampleRate": "100000", "640 Shutter-IOChannel": "Dev1/ao14", "555 AO-Sequenceable": "1", "520 AO-Name": "AnalogIO", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "470 AO-Block voltage": "false", "COM1-Verbose": "1", "COM9-AnswerTimeout": "10000.0000", "528 Shutter-Name": "AnalogIO", "PRIME-Trigger Timeout (secs)": "10", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "NextFrame": 0, "470 AO-TriggerSequenceLength": "1000", "PRIME-TransposeXY": "0", "445 AO-TriggerSequenceLength": "1000", "COM1-Name": "COM1", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "COM9-DataBits": "8", "COM1-AnswerTimeout": "500.0000", "Camera": "", "TE2000-Version": "V2.50-V2.50-V2.10", "528 Shutter-Volts": "0.0000", "XLIGHT Hub-Name": "XLIGHT Hub", "PIEZO-Description": "Ludl stage driver adapter", "PositionName": "Pos17", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSampleRate": "100000", "PRIME-FanSpeedSetpoint": "High", "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "405 AO-TriggerSampleRate": "100000", "470 Shutter-Block voltage": "false", "470-State": "0", "COM3-Parity": "None", "405 AO-Volts": "0.0000", "PRIME-TransposeMirrorY": "0", "PRIME-ChipName": "GS144BSI", "470 AO-Volts": "0.0000", "PRIME-TransposeMirrorX": "0", "445 Shutter-SupportsTriggering": "0", "470 Shutter-SupportsTriggering": "0", "XLIGHT Hub-Description": "XLIGHT hub", "PRIME-PreampOffLimit": "10000", "470 AO-Sequenceable": "1", "528 Shutter-MaxVolts": "5.0000", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "Core-XYStage": "XYStage", "470 AO-TriggerSampleRate": "100000", "PRIME-Timing-PreTriggerDelayNs": "24672000.0000", "528-DA Device": "528 Shutter", "Focus-Description": "Nikon TE2000 focus adapter", "FilterBlock-State": "5", "520 AO-Demo": "No", "COM3-DelayBetweenCharsMs": "0.0000", "470 Shutter-Volts": "0.0000", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "PRIME-PP 2 THRESHOLD": "125", "PRIME-PP 4 THRESHOLD": "75", "XYStage-Acceleration": "4.0000", "470 Shutter-Demo": "No", "PRIME-Name": "PMPCIECam00", "640-DA Device": "640 Shutter", "405 AO-Block voltage": "false", "ElapsedTime-ms": 743978, "PRIME-Offset": "100", "445 AO-IOChannel": "Dev1/ao1", "640 AO-MaxVolts": "5.0000", "PositionIndex": 17, "PRIME-PP 1 ENABLED": "Yes", "COM1-BaudRate": "9600", "640-State": "0", "COM3-Verbose": "1", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "LudlController-TransmissionDelay": "0", "PRIME-FullWellCapacity": "62000", "PRIME-SMARTStreamingEnabled": "No", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "640 Shutter-Description": "NI DAC", "528-Description": "DA device that is used as a shutter", "COM4-Description": "Serial port driver (boost:asio)", "405 Shutter-Description": "NI DAC", "TE2000-Name": "TE2000", "528 Shutter-Description": "NI DAC", "COM9-Description": "Serial port driver (boost:asio)", "LudlController-Name": "LudlController", "445 Shutter-TriggerSequenceLength": "1000", "PRIME-SerialNumber": "A18B203008", "445 AO-Volts": "0.0000", "PRIME-X-dimension": "1200", "PRIME-ShutterCloseDelay": "0", "XLIGHT Disk slider-State": "2", "Position": "Default", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 AO-IOChannel": "Dev1/ao5", "520 AO-IOChannel": "Dev1/ao4", "528 AO-TriggerSequenceLength": "1000", "405 Shutter-MaxVolts": "5.0000", "470-DA Device": "470 Shutter", "PRIME-FTCapable": "No", "Core-ChannelGroup": "Channels", "555 AO-SupportsTriggering": "0", "PRIME-Y-dimension": "1200", "Frame": 0, "555 Shutter-Sequenceable": "1", "PRIME-Timing-ExposureTimeNs": "499998000.0000", "Core-ImageProcessor": "", "528-State": "0", "520 AO-Block voltage": "false", "XLIGHT Excitation Wheel-Label": "Excitation-2", "640 Shutter-Demo": "No", "COM3-StopBits": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "520 Shutter-IOChannel": "Dev1/ao11", "Width": 1200, "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-SupportsTriggering": "0", "640 AO-TriggerSampleRate": "100000", "520-Description": "DA device that is used as a shutter", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "405 Shutter-Sequenceable": "1", "405 Shutter-SupportsTriggering": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-TriggerSampleRate": "100000", "640 Shutter-MaxVolts": "5.0000", "555 AO-MaxVolts": "5.0000", "520 AO-TriggerSequenceLength": "1000", "COM3-BaudRate": "9600", "COM9-BaudRate": "9600", "Vincent-D3-Channel #": "Ch. #1", "PRIME-PMode": "Normal", "OpticalPath-Label": "Left(100)", "BitDepth": 16, "555 AO-TriggerSequenceLength": "1000", "445 Shutter-Description": "NI DAC", "405 AO-Sequenceable": "1", "PRIME-AcquisitionMethod": "Callbacks", "555 AO-Name": "AnalogIO", "PRIME-ShutterMode": "Pre-Exposure", "COM3-Handshaking": "Off", "445 AO-SupportsTriggering": "0", "ROI": "0-0-1200-1200", "COM9-Verbose": "1", "PRIME-Color": "OFF", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "PRIME-ShutterOpenDelay": "0", "COM1-Parity": "None", "Slice": 0, "SlicePosition": -50, "COM9-DelayBetweenCharsMs": "0.0000", "555-Name": "DA Shutter", "PIEZO-LudlSingleAxisName": "B", "XLIGHT Emission Wheel-Label": "Empty", "640 Shutter-Name": "AnalogIO", "640 AO-Sequenceable": "1", "520 Shutter-Demo": "No", "528 Shutter-Demo": "No", "470-Name": "DA Shutter", "520-DA Device": "520 Shutter", "XYStage-Description": "Ludl XY stage driver adapter", "445 Shutter-MinVolts": "0.0000", "528 AO-TriggerSampleRate": "100000", "405 AO-MinVolts": "0.0000", "405 Shutter-TriggerSequenceLength": "1000", "Height": 1200, "555 AO-Block voltage": "false", "640 Shutter-Volts": "0.0000", "PixelType": "GRAY16", "445 Shutter-Block voltage": "false", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "520 Shutter-TriggerSequenceLength": "1000", "COM9-Name": "COM9", "Core-SLM": "", "555 AO-Volts": "0.0000", "555 Shutter-Block voltage": "false", "XYStage-Speed": "1800.0000", "520 AO-MaxVolts": "5.0000", "528 Shutter-SupportsTriggering": "0", "COM1-Description": "Serial port driver (boost:asio)", "PRIME-MetadataEnabled": "No", "520 Shutter-MinVolts": "0.0000", "470 Shutter-Sequenceable": "1", "PRIME-PreampDelay": "0", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-MinVolts": "0.0000", "ChannelIndex": 4, "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Core-AutoShutter": "1", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "Exposure-ms": 20, "PRIME-PP 2 ENABLED": "Yes", "640 Shutter-TriggerSampleRate": "100000", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "470 Shutter-IOChannel": "Dev1/ao10", "405 AO-Demo": "No", "PixelSizeUm": 1.1, "470 AO-Demo": "No", "470 Shutter-MaxVolts": "5.0000", "Vincent-D3-Name": "Vincent-D3", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FileName": "img_000000000_P-DIC_000.tif", "LudlController-Reset": "Operate", "PRIME-Exposure": "20.00", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "445-Name": "DA Shutter", "555 Shutter-Name": "AnalogIO", "520 Shutter-TriggerSampleRate": "100000", "445 Shutter-IOChannel": "Dev1/ao9", "PRIME-TransposeCorrection": "0", "XYStage-StepSize": "0.1000", "445-Description": "DA device that is used as a shutter", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 AO-IOChannel": "Dev1/ao7", "520 Shutter-Name": "AnalogIO", "COM3-DataBits": "8", "640 AO-Volts": "0.0000", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "555 Shutter-IOChannel": "Dev1/ao13", "WaitInterval": null, "520 Shutter-Sequenceable": "1", "YPositionUm": -13012.300000000001, "445-State": "0", "445-DA Device": "445 Shutter", "PRIME-CircularBufferAutoSize": "ON", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "555 AO-TriggerSampleRate": "100000", "445 Shutter-Volts": "0.0000", "XLIGHT Hub-Port": "COM3", "Core-AutoFocus": "", "COM4-Handshaking": "Off", "528 Shutter-MinVolts": "0.0000", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "445 Shutter-Name": "AnalogIO", "528 Shutter-Sequenceable": "1", "XYStage-TransposeMirrorX": "0", "XYStage-TransposeMirrorY": "0", "PRIME-ReadoutRate": "100MHz 16bit", "XYStage-Name": "Stage", "640 AO-SupportsTriggering": "0", "640-Name": "DA Shutter", "640 Shutter-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "640 AO-Block voltage": "false", "445 Shutter-Sequenceable": "1", "470 AO-MaxVolts": "5.0000", "COM4-BaudRate": "9600", "470-Description": "DA device that is used as a shutter", "520 Shutter-Description": "NI DAC", "PRIME-CCDTemperature": "-14.9200", "Time": "2019-12-17 04:00:00 -0500", "640 AO-TriggerSequenceLength": "1000", "470 AO-Name": "AnalogIO", "XLIGHT Disk slider-Label": "Disk Pos 40um", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "Binning": "1x1", "TE2000-ModelType": "1", "COM3-AnswerTimeout": "500.0000", "555 Shutter-SupportsTriggering": "0", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "TE2000-Port": "COM4", "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Description": "NI DAC", "COM4-AnswerTimeout": "500.0000", "COM1-DelayBetweenCharsMs": "0.0000", "FilterBlock-Label": "Confocal", "445 AO-Name": "AnalogIO", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-Timing-ReadoutTimeNs": "24672000.0000", "528 AO-Demo": "No", "520 AO-Sequenceable": "1", "555-Description": "DA device that is used as a shutter", "405-DA Device": "405 Shutter", "405 AO-TriggerSequenceLength": "1000", "640 AO-MinVolts": "0.0000", "640 Shutter-Block voltage": "false", "FrameIndex": 0, "PRIME-Port": "CMOS", "555 AO-MinVolts": "0.0000", "COM3-Description": "Serial port driver (boost:asio)", "528 AO-Description": "NI DAC", "555 AO-IOChannel": "Dev1/ao6", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "555 AO-TriggerInputLine": "/Dev1/PFI0", "PRIME-ActualInterval-ms": "84.6200", "LudlController-Port": "COM9", "445 Shutter-Demo": "No", "PRIME-Trigger-Expose Out-Mux": "1", "SliceIndex": 0, "PRIME-CCDTemperatureSetPoint": "-15.0000", "405 Shutter-MinVolts": "0.0000", "PRIME-ExposeOutMode": "First Row", "AxisPositions": null, "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Volts": "0.0000", "528-Name": "DA Shutter", "555-State": "0", "XLIGHT Dichroic-Label": "Di03-R635-t1", "445 AO-Sequenceable": "1", "405-Name": "DA Shutter", "405 AO-IOChannel": "Dev1/ao0", "Core-Camera": "PRIME", "Focus-Name": "Focus", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "405 Shutter-IOChannel": "Dev1/ao8", "640 AO-Demo": "No", "640 Shutter-SupportsTriggering": "0", "445 AO-Demo": "No", "555-DA Device": "555 Shutter", "470 Shutter-Description": "NI DAC", "528 AO-Volts": "0.0000", "COM1-DataBits": "8", "Vincent-D3-Address": "x", "520 AO-SupportsTriggering": "0", "405-Description": "DA device that is used as a shutter", "640 Shutter-MinVolts": "0.0000", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "405 AO-Name": "AnalogIO", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "640 AO-TriggerInputLine": "/Dev1/PFI0", "405 AO-TriggerInputLine": "/Dev1/PFI0", "640-Description": "DA device that is used as a shutter", "445 Shutter-TriggerSampleRate": "100000", "470 Shutter-TriggerSequenceLength": "1000", "COM1-StopBits": "1", "520 Shutter-SupportsTriggering": "0", "405-State": "0", "Core-Initialize": "1", "XLIGHT Emission Wheel-State": "3", "520 AO-Description": "NI DAC", "528 AO-TriggerInputLine": "/Dev1/PFI0", "445 Shutter-MaxVolts": "5.0000", "COM9-Handshaking": "Off", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "XLIGHT Spin motor-Label": "On (spinning)", "PRIME-PP 5 ENABLED": "No", "UUID": "18c105dc-a4f6-47e3-adf0-493266d07482", "PRIME-TriggerMode": "Internal Trigger", "COM1-Handshaking": "Off", "PRIME-Timing-PostTriggerDelayNs": "49344000.0000", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "Focus-Position": "16732.9000", "528 Shutter-Block voltage": "false", "Channel": "P-DIC", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "555 Shutter-Demo": "No", "405 Shutter-Demo": "No", "528 AO-Name": "AnalogIO", "520-State": "0", "Core-Galvo": "", "405 Shutter-TriggerSampleRate": "100000", "Focus-FocusStepSize_(nm)": "100", "PRIME-PixelType": "16bit", "COM4-DataBits": "8", "470 AO-IOChannel": "Dev1/ao2", "520 AO-MinVolts": "0.0000", "COM9-Parity": "None", "PRIME-CircularBufferEnabled": "ON", "PRIME-PP 6 Reset": "No", "640 AO-Description": "NI DAC", "Source": "PRIME", "445 AO-MinVolts": "0.0000", "PIEZO-Name": "Stage", "445 AO-Description": "NI DAC", "PRIME-CircularBufferFrameCount": "46", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "520 AO-Volts": "0.0000", "528 Shutter-TriggerSampleRate": "100000", "XPositionUm": 23269.100000000002, "OpticalPath-State": "4", "PRIME-PP 3 THRESHOLD": "80", "PRIME-PVCAM Version": "3.7.1", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Gain": "1-HDR", "640 Shutter-TriggerSequenceLength": "1000", "528 AO-SupportsTriggering": "0", "445 AO-TriggerInputLine": "/Dev1/PFI0", "OpticalPath-Name": "OpticalPath", "PRIME-PP 4 ENABLED": "Yes", "PRIME-PVCAM Adapter Version": "1.3.29", "COM3-Name": "COM3", "405 AO-MaxVolts": "5.0000", "520 Shutter-Block voltage": "false", "PRIME-Timing-ClearingTimeNs": "49344000.0000", "Core-Focus": "PIEZO", "528 AO-Block voltage": "false", "TE2000-Description": "Nikon TE2000 hub", "528 AO-MinVolts": "0.0000", "COM4-Parity": "None", "Summary": { "Slices": 11, "ChContrastMin": [ 0, 0, 0, 0, 0, 0 ], "PixelAspect": 1, "SlicesFirst": true, "Interval_ms": 0, "Time": "2019-12-17 03:59:33 -0500", "Prefix": "seq_7", "InitialPositionList": [ { "Label": "Pos0", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [149.91], "Focus": [16657.7], "XYStage": [ 71629.7, 19152.9 ] }, "GridColumnIndex": 0 }, { "Label": "Pos1", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [190.82], "Focus": [16485.4], "XYStage": [ 65702.3, 19396.600000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos2", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [194.69], "Focus": [16446.8], "XYStage": [ 59880.5, 19007.8 ] }, "GridColumnIndex": 0 }, { "Label": "Pos3", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [145.04], "Focus": [16446.6], "XYStage": [ 53616.9, 18818.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos4", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [169.57500000000002], "Focus": [16446.5], "XYStage": [ 47662.8, 18817.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos5", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [131.805], "Focus": [16446.4], "XYStage": [ 41918.8, 18298.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos6", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [113.145], "Focus": [16446.5], "XYStage": [ 36129.9, 18895.8 ] }, "GridColumnIndex": 0 }, { "Label": "Pos7", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [141.005], "Focus": [16446.6], "XYStage": [ 30299.100000000002, 18895.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos8", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [140.97], "Focus": [16446.5], "XYStage": [ 23952.100000000002, 19156 ] }, "GridColumnIndex": 0 }, { "Label": "Pos9", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [184.57500000000002], "Focus": [16677.9], "XYStage": [ 72866.8, -14809.300000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos10", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [167.95000000000002], "Focus": [16557.9], "XYStage": [ 66515.6, -14371.300000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos11", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [168], "Focus": [16618.9], "XYStage": [ 59724.3, -13964.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos12", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [203.205], "Focus": [16779.6], "XYStage": [ 53862.3, -13494.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos13", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [203.20000000000002], "Focus": [16749], "XYStage": [ 48108.3, -13805.400000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos14", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [203.19], "Focus": [16675.9], "XYStage": [ 41645, -13804.900000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos15", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [203.21], "Focus": [16675.7], "XYStage": [ 35352.6, -13805.6 ] }, "GridColumnIndex": 0 }, { "Label": "Pos16", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [203.225], "Focus": [16675.8], "XYStage": [ 29298, -13447.400000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos17", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [203.215], "Focus": [16675.8], "XYStage": [ 23269.100000000002, -13012.300000000001 ] }, "GridColumnIndex": 0 } ], "z-step_um": 10, "GridRow": 0, "BitDepth": 16, "Depth": 2, "Channels": 6, "Source": "Micro-Manager", "ChColors": [ -16711681, -256, -65281, -1, -1, -16776961 ], "MetadataVersion": 10, "KeepShutterOpenChannels": true, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536, 65536 ], "CustomIntervals_ms": [], "Height": 1200, "MicroManagerVersion": "1.4.23 20180219", "GridColumn": 0, "UUID": "3ad8801b-5699-40d6-813b-4ad840863e38", "PixelType": "GRAY16", "Width": 1200, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\Yan\\20191216-YC113-S11S12-miseq", "Frames": 1, "Comment": "", "UserName": "Nikon", "PixelSize_um": 1.1, "ROI": [ 0, 0, 1200, 1200 ], "Date": "2019-12-17", "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC", "P-DAPI-high" ], "KeepShutterOpenSlices": true, "IJType": 1, "ComputerName": "ZadorConfocal2", "Positions": 18, "PositionIndex": 17, "CameraTimeout": 20000, "TimeFirst": false }, "640 AO-Name": "AnalogIO", "Vincent-D3-Command": "Close" }, "FrameKey-0-4-1": { "COM4-StopBits": "1", "528 AO-MaxVolts": "5.0000", "XLIGHT Excitation Wheel-State": "1", "470 Shutter-Name": "AnalogIO", "Core-Shutter": "Vincent-D3", "555 AO-Demo": "No", "XLIGHT Dichroic-State": "2", "XYStage-StepSize-Y": "0.1000", "ZPositionUm": 163.215, "XYStage-StepSize-X": "0.1000", "PRIME-FirmwareVersion": "20.28", "PRIME-ClearCycles": "2", "520-Name": "DA Shutter", "405 Shutter-Name": "AnalogIO", "PIEZO-StepSize": "0.0050", "PRIME-ClearMode": "Pre-Exposure", "470 AO-MinVolts": "0.0000", "405 AO-SupportsTriggering": "1", "CameraChannelIndex": 0, "XYStage-StartSpeed": "500.0000", "COM9-StopBits": "2", "405 AO-Description": "NI DAC", "COM4-DelayBetweenCharsMs": "0.0000", "445 AO-Block voltage": "false", "520 AO-TriggerInputLine": "/Dev1/PFI0", "555 AO-Description": "NI DAC", "520 AO-TriggerSampleRate": "100000", "COM4-Verbose": "1", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM4-Name": "COM4", "405 Shutter-Volts": "0.0000", "Vincent-D3-Port": "COM1", "PRIME-PP 4 MIN ADU AFFECTED": "200", "470 Shutter-MinVolts": "0.0000", "PRIME-PP 3 ENABLED": "Yes", "470 AO-Description": "NI DAC", "PRIME-Description": "PVCAM API device adapter", "520 Shutter-MaxVolts": "5.0000", "520 Shutter-Volts": "0.0000", "528 AO-Sequenceable": "1", "PRIME-Binning": "1x1", "555 Shutter-MaxVolts": "5.0000", "528 Shutter-TriggerSequenceLength": "1000", "405 Shutter-Block voltage": "false", "XLIGHT Spin motor-State": "1", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "Vincent-D3-Time to open (ms)": "35.0000", "Vincent-D3-Time to close (ms)": "35.0000", "445 AO-TriggerSampleRate": "100000", "640 Shutter-IOChannel": "Dev1/ao14", "555 AO-Sequenceable": "1", "520 AO-Name": "AnalogIO", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "470 AO-Block voltage": "false", "COM1-Verbose": "1", "COM9-AnswerTimeout": "10000.0000", "528 Shutter-Name": "AnalogIO", "PRIME-Trigger Timeout (secs)": "10", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "NextFrame": 0, "470 AO-TriggerSequenceLength": "1000", "PRIME-TransposeXY": "0", "445 AO-TriggerSequenceLength": "1000", "COM1-Name": "COM1", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "COM9-DataBits": "8", "COM1-AnswerTimeout": "500.0000", "Camera": "", "TE2000-Version": "V2.50-V2.50-V2.10", "528 Shutter-Volts": "0.0000", "XLIGHT Hub-Name": "XLIGHT Hub", "PIEZO-Description": "Ludl stage driver adapter", "PositionName": "Pos17", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSampleRate": "100000", "PRIME-FanSpeedSetpoint": "High", "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "405 AO-TriggerSampleRate": "100000", "470 Shutter-Block voltage": "false", "470-State": "0", "COM3-Parity": "None", "405 AO-Volts": "0.0000", "PRIME-TransposeMirrorY": "0", "PRIME-ChipName": "GS144BSI", "470 AO-Volts": "0.0000", "PRIME-TransposeMirrorX": "0", "445 Shutter-SupportsTriggering": "0", "470 Shutter-SupportsTriggering": "0", "XLIGHT Hub-Description": "XLIGHT hub", "PRIME-PreampOffLimit": "10000", "470 AO-Sequenceable": "1", "528 Shutter-MaxVolts": "5.0000", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "Core-XYStage": "XYStage", "470 AO-TriggerSampleRate": "100000", "PRIME-Timing-PreTriggerDelayNs": "24672000.0000", "528-DA Device": "528 Shutter", "Focus-Description": "Nikon TE2000 focus adapter", "FilterBlock-State": "5", "520 AO-Demo": "No", "COM3-DelayBetweenCharsMs": "0.0000", "470 Shutter-Volts": "0.0000", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "PRIME-PP 2 THRESHOLD": "125", "PRIME-PP 4 THRESHOLD": "75", "XYStage-Acceleration": "4.0000", "470 Shutter-Demo": "No", "PRIME-Name": "PMPCIECam00", "640-DA Device": "640 Shutter", "405 AO-Block voltage": "false", "ElapsedTime-ms": 744287, "PRIME-Offset": "100", "445 AO-IOChannel": "Dev1/ao1", "640 AO-MaxVolts": "5.0000", "PositionIndex": 17, "PRIME-PP 1 ENABLED": "Yes", "COM1-BaudRate": "9600", "640-State": "0", "COM3-Verbose": "1", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "LudlController-TransmissionDelay": "0", "PRIME-FullWellCapacity": "62000", "PRIME-SMARTStreamingEnabled": "No", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "640 Shutter-Description": "NI DAC", "528-Description": "DA device that is used as a shutter", "COM4-Description": "Serial port driver (boost:asio)", "405 Shutter-Description": "NI DAC", "TE2000-Name": "TE2000", "528 Shutter-Description": "NI DAC", "COM9-Description": "Serial port driver (boost:asio)", "LudlController-Name": "LudlController", "445 Shutter-TriggerSequenceLength": "1000", "PRIME-SerialNumber": "A18B203008", "445 AO-Volts": "0.0000", "PRIME-X-dimension": "1200", "PRIME-ShutterCloseDelay": "0", "XLIGHT Disk slider-State": "2", "Position": "Default", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 AO-IOChannel": "Dev1/ao5", "520 AO-IOChannel": "Dev1/ao4", "528 AO-TriggerSequenceLength": "1000", "405 Shutter-MaxVolts": "5.0000", "470-DA Device": "470 Shutter", "PRIME-FTCapable": "No", "Core-ChannelGroup": "Channels", "555 AO-SupportsTriggering": "0", "PRIME-Y-dimension": "1200", "Frame": 0, "555 Shutter-Sequenceable": "1", "PRIME-Timing-ExposureTimeNs": "499998000.0000", "Core-ImageProcessor": "", "528-State": "0", "520 AO-Block voltage": "false", "XLIGHT Excitation Wheel-Label": "Excitation-2", "640 Shutter-Demo": "No", "COM3-StopBits": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "520 Shutter-IOChannel": "Dev1/ao11", "Width": 1200, "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-SupportsTriggering": "0", "640 AO-TriggerSampleRate": "100000", "520-Description": "DA device that is used as a shutter", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "405 Shutter-Sequenceable": "1", "405 Shutter-SupportsTriggering": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-TriggerSampleRate": "100000", "640 Shutter-MaxVolts": "5.0000", "555 AO-MaxVolts": "5.0000", "520 AO-TriggerSequenceLength": "1000", "COM3-BaudRate": "9600", "COM9-BaudRate": "9600", "Vincent-D3-Channel #": "Ch. #1", "PRIME-PMode": "Normal", "OpticalPath-Label": "Left(100)", "BitDepth": 16, "555 AO-TriggerSequenceLength": "1000", "445 Shutter-Description": "NI DAC", "405 AO-Sequenceable": "1", "PRIME-AcquisitionMethod": "Callbacks", "555 AO-Name": "AnalogIO", "PRIME-ShutterMode": "Pre-Exposure", "COM3-Handshaking": "Off", "445 AO-SupportsTriggering": "0", "ROI": "0-0-1200-1200", "COM9-Verbose": "1", "PRIME-Color": "OFF", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "PRIME-ShutterOpenDelay": "0", "COM1-Parity": "None", "Slice": 1, "SlicePosition": -40, "COM9-DelayBetweenCharsMs": "0.0000", "555-Name": "DA Shutter", "PIEZO-LudlSingleAxisName": "B", "XLIGHT Emission Wheel-Label": "Empty", "640 Shutter-Name": "AnalogIO", "640 AO-Sequenceable": "1", "520 Shutter-Demo": "No", "528 Shutter-Demo": "No", "470-Name": "DA Shutter", "520-DA Device": "520 Shutter", "XYStage-Description": "Ludl XY stage driver adapter", "445 Shutter-MinVolts": "0.0000", "528 AO-TriggerSampleRate": "100000", "405 AO-MinVolts": "0.0000", "405 Shutter-TriggerSequenceLength": "1000", "Height": 1200, "555 AO-Block voltage": "false", "640 Shutter-Volts": "0.0000", "PixelType": "GRAY16", "445 Shutter-Block voltage": "false", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "520 Shutter-TriggerSequenceLength": "1000", "COM9-Name": "COM9", "Core-SLM": "", "555 AO-Volts": "0.0000", "555 Shutter-Block voltage": "false", "XYStage-Speed": "1800.0000", "520 AO-MaxVolts": "5.0000", "528 Shutter-SupportsTriggering": "0", "COM1-Description": "Serial port driver (boost:asio)", "PRIME-MetadataEnabled": "No", "520 Shutter-MinVolts": "0.0000", "470 Shutter-Sequenceable": "1", "PRIME-PreampDelay": "0", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-MinVolts": "0.0000", "ChannelIndex": 4, "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Core-AutoShutter": "1", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "Exposure-ms": 20, "PRIME-PP 2 ENABLED": "Yes", "640 Shutter-TriggerSampleRate": "100000", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "470 Shutter-IOChannel": "Dev1/ao10", "405 AO-Demo": "No", "PixelSizeUm": 1.1, "470 AO-Demo": "No", "470 Shutter-MaxVolts": "5.0000", "Vincent-D3-Name": "Vincent-D3", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FileName": "img_000000000_P-DIC_001.tif", "LudlController-Reset": "Operate", "PRIME-Exposure": "20.00", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "445-Name": "DA Shutter", "555 Shutter-Name": "AnalogIO", "520 Shutter-TriggerSampleRate": "100000", "445 Shutter-IOChannel": "Dev1/ao9", "PRIME-TransposeCorrection": "0", "XYStage-StepSize": "0.1000", "445-Description": "DA device that is used as a shutter", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 AO-IOChannel": "Dev1/ao7", "520 Shutter-Name": "AnalogIO", "COM3-DataBits": "8", "640 AO-Volts": "0.0000", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "555 Shutter-IOChannel": "Dev1/ao13", "WaitInterval": null, "520 Shutter-Sequenceable": "1", "YPositionUm": -13012.300000000001, "445-State": "0", "445-DA Device": "445 Shutter", "PRIME-CircularBufferAutoSize": "ON", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "555 AO-TriggerSampleRate": "100000", "445 Shutter-Volts": "0.0000", "XLIGHT Hub-Port": "COM3", "Core-AutoFocus": "", "COM4-Handshaking": "Off", "528 Shutter-MinVolts": "0.0000", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "445 Shutter-Name": "AnalogIO", "528 Shutter-Sequenceable": "1", "XYStage-TransposeMirrorX": "0", "XYStage-TransposeMirrorY": "0", "PRIME-ReadoutRate": "100MHz 16bit", "XYStage-Name": "Stage", "640 AO-SupportsTriggering": "0", "640-Name": "DA Shutter", "640 Shutter-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "640 AO-Block voltage": "false", "445 Shutter-Sequenceable": "1", "470 AO-MaxVolts": "5.0000", "COM4-BaudRate": "9600", "470-Description": "DA device that is used as a shutter", "520 Shutter-Description": "NI DAC", "PRIME-CCDTemperature": "-14.9200", "Time": "2019-12-17 04:00:01 -0500", "640 AO-TriggerSequenceLength": "1000", "470 AO-Name": "AnalogIO", "XLIGHT Disk slider-Label": "Disk Pos 40um", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "Binning": "1x1", "TE2000-ModelType": "1", "COM3-AnswerTimeout": "500.0000", "555 Shutter-SupportsTriggering": "0", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "TE2000-Port": "COM4", "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Description": "NI DAC", "COM4-AnswerTimeout": "500.0000", "COM1-DelayBetweenCharsMs": "0.0000", "FilterBlock-Label": "Confocal", "445 AO-Name": "AnalogIO", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-Timing-ReadoutTimeNs": "24672000.0000", "528 AO-Demo": "No", "520 AO-Sequenceable": "1", "555-Description": "DA device that is used as a shutter", "405-DA Device": "405 Shutter", "405 AO-TriggerSequenceLength": "1000", "640 AO-MinVolts": "0.0000", "640 Shutter-Block voltage": "false", "FrameIndex": 0, "PRIME-Port": "CMOS", "555 AO-MinVolts": "0.0000", "COM3-Description": "Serial port driver (boost:asio)", "528 AO-Description": "NI DAC", "555 AO-IOChannel": "Dev1/ao6", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "555 AO-TriggerInputLine": "/Dev1/PFI0", "PRIME-ActualInterval-ms": "84.6200", "LudlController-Port": "COM9", "445 Shutter-Demo": "No", "PRIME-Trigger-Expose Out-Mux": "1", "SliceIndex": 1, "PRIME-CCDTemperatureSetPoint": "-15.0000", "405 Shutter-MinVolts": "0.0000", "PRIME-ExposeOutMode": "First Row", "AxisPositions": null, "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Volts": "0.0000", "528-Name": "DA Shutter", "555-State": "0", "XLIGHT Dichroic-Label": "Di03-R635-t1", "445 AO-Sequenceable": "1", "405-Name": "DA Shutter", "405 AO-IOChannel": "Dev1/ao0", "Core-Camera": "PRIME", "Focus-Name": "Focus", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "405 Shutter-IOChannel": "Dev1/ao8", "640 AO-Demo": "No", "640 Shutter-SupportsTriggering": "0", "445 AO-Demo": "No", "555-DA Device": "555 Shutter", "470 Shutter-Description": "NI DAC", "528 AO-Volts": "0.0000", "COM1-DataBits": "8", "Vincent-D3-Address": "x", "520 AO-SupportsTriggering": "0", "405-Description": "DA device that is used as a shutter", "640 Shutter-MinVolts": "0.0000", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "405 AO-Name": "AnalogIO", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "640 AO-TriggerInputLine": "/Dev1/PFI0", "405 AO-TriggerInputLine": "/Dev1/PFI0", "640-Description": "DA device that is used as a shutter", "445 Shutter-TriggerSampleRate": "100000", "470 Shutter-TriggerSequenceLength": "1000", "COM1-StopBits": "1", "520 Shutter-SupportsTriggering": "0", "405-State": "0", "Core-Initialize": "1", "XLIGHT Emission Wheel-State": "3", "520 AO-Description": "NI DAC", "528 AO-TriggerInputLine": "/Dev1/PFI0", "445 Shutter-MaxVolts": "5.0000", "COM9-Handshaking": "Off", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "XLIGHT Spin motor-Label": "On (spinning)", "PRIME-PP 5 ENABLED": "No", "UUID": "cbc9eb10-fca0-4286-8e6b-2ac9e0517350", "PRIME-TriggerMode": "Internal Trigger", "COM1-Handshaking": "Off", "PRIME-Timing-PostTriggerDelayNs": "49344000.0000", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "Focus-Position": "16732.9000", "528 Shutter-Block voltage": "false", "Channel": "P-DIC", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "555 Shutter-Demo": "No", "405 Shutter-Demo": "No", "528 AO-Name": "AnalogIO", "520-State": "0", "Core-Galvo": "", "405 Shutter-TriggerSampleRate": "100000", "Focus-FocusStepSize_(nm)": "100", "PRIME-PixelType": "16bit", "COM4-DataBits": "8", "470 AO-IOChannel": "Dev1/ao2", "520 AO-MinVolts": "0.0000", "COM9-Parity": "None", "PRIME-CircularBufferEnabled": "ON", "PRIME-PP 6 Reset": "No", "640 AO-Description": "NI DAC", "Source": "PRIME", "445 AO-MinVolts": "0.0000", "PIEZO-Name": "Stage", "445 AO-Description": "NI DAC", "PRIME-CircularBufferFrameCount": "46", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "520 AO-Volts": "0.0000", "528 Shutter-TriggerSampleRate": "100000", "XPositionUm": 23269.100000000002, "OpticalPath-State": "4", "PRIME-PP 3 THRESHOLD": "80", "PRIME-PVCAM Version": "3.7.1", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Gain": "1-HDR", "640 Shutter-TriggerSequenceLength": "1000", "528 AO-SupportsTriggering": "0", "445 AO-TriggerInputLine": "/Dev1/PFI0", "OpticalPath-Name": "OpticalPath", "PRIME-PP 4 ENABLED": "Yes", "PRIME-PVCAM Adapter Version": "1.3.29", "COM3-Name": "COM3", "405 AO-MaxVolts": "5.0000", "520 Shutter-Block voltage": "false", "PRIME-Timing-ClearingTimeNs": "49344000.0000", "Core-Focus": "PIEZO", "528 AO-Block voltage": "false", "TE2000-Description": "Nikon TE2000 hub", "528 AO-MinVolts": "0.0000", "COM4-Parity": "None", "Summary": { "Slices": 11, "ChContrastMin": [ 0, 0, 0, 0, 0, 0 ], "PixelAspect": 1, "SlicesFirst": true, "Interval_ms": 0, "Time": "2019-12-17 03:59:33 -0500", "Prefix": "seq_7", "InitialPositionList": [ { "Label": "Pos0", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [149.91], "Focus": [16657.7], "XYStage": [ 71629.7, 19152.9 ] }, "GridColumnIndex": 0 }, { "Label": "Pos1", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [190.82], "Focus": [16485.4], "XYStage": [ 65702.3, 19396.600000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos2", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [194.69], "Focus": [16446.8], "XYStage": [ 59880.5, 19007.8 ] }, "GridColumnIndex": 0 }, { "Label": "Pos3", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [145.04], "Focus": [16446.6], "XYStage": [ 53616.9, 18818.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos4", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [169.57500000000002], "Focus": [16446.5], "XYStage": [ 47662.8, 18817.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos5", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [131.805], "Focus": [16446.4], "XYStage": [ 41918.8, 18298.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos6", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [113.145], "Focus": [16446.5], "XYStage": [ 36129.9, 18895.8 ] }, "GridColumnIndex": 0 }, { "Label": "Pos7", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [141.005], "Focus": [16446.6], "XYStage": [ 30299.100000000002, 18895.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos8", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [140.97], "Focus": [16446.5], "XYStage": [ 23952.100000000002, 19156 ] }, "GridColumnIndex": 0 }, { "Label": "Pos9", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [184.57500000000002], "Focus": [16677.9], "XYStage": [ 72866.8, -14809.300000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos10", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [167.95000000000002], "Focus": [16557.9], "XYStage": [ 66515.6, -14371.300000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos11", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [168], "Focus": [16618.9], "XYStage": [ 59724.3, -13964.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos12", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [203.205], "Focus": [16779.6], "XYStage": [ 53862.3, -13494.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos13", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [203.20000000000002], "Focus": [16749], "XYStage": [ 48108.3, -13805.400000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos14", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [203.19], "Focus": [16675.9], "XYStage": [ 41645, -13804.900000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos15", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [203.21], "Focus": [16675.7], "XYStage": [ 35352.6, -13805.6 ] }, "GridColumnIndex": 0 }, { "Label": "Pos16", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [203.225], "Focus": [16675.8], "XYStage": [ 29298, -13447.400000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos17", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [203.215], "Focus": [16675.8], "XYStage": [ 23269.100000000002, -13012.300000000001 ] }, "GridColumnIndex": 0 } ], "z-step_um": 10, "GridRow": 0, "BitDepth": 16, "Depth": 2, "Channels": 6, "Source": "Micro-Manager", "ChColors": [ -16711681, -256, -65281, -1, -1, -16776961 ], "MetadataVersion": 10, "KeepShutterOpenChannels": true, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536, 65536 ], "CustomIntervals_ms": [], "Height": 1200, "MicroManagerVersion": "1.4.23 20180219", "GridColumn": 0, "UUID": "3ad8801b-5699-40d6-813b-4ad840863e38", "PixelType": "GRAY16", "Width": 1200, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\Yan\\20191216-YC113-S11S12-miseq", "Frames": 1, "Comment": "", "UserName": "Nikon", "PixelSize_um": 1.1, "ROI": [ 0, 0, 1200, 1200 ], "Date": "2019-12-17", "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC", "P-DAPI-high" ], "KeepShutterOpenSlices": true, "IJType": 1, "ComputerName": "ZadorConfocal2", "Positions": 18, "PositionIndex": 17, "CameraTimeout": 20000, "TimeFirst": false }, "640 AO-Name": "AnalogIO", "Vincent-D3-Command": "Close" }, "FrameKey-0-4-2": { "COM4-StopBits": "1", "528 AO-MaxVolts": "5.0000", "XLIGHT Excitation Wheel-State": "1", "470 Shutter-Name": "AnalogIO", "Core-Shutter": "Vincent-D3", "555 AO-Demo": "No", "XLIGHT Dichroic-State": "2", "XYStage-StepSize-Y": "0.1000", "ZPositionUm": 173.215, "XYStage-StepSize-X": "0.1000", "PRIME-FirmwareVersion": "20.28", "PRIME-ClearCycles": "2", "520-Name": "DA Shutter", "405 Shutter-Name": "AnalogIO", "PIEZO-StepSize": "0.0050", "PRIME-ClearMode": "Pre-Exposure", "470 AO-MinVolts": "0.0000", "405 AO-SupportsTriggering": "1", "CameraChannelIndex": 0, "XYStage-StartSpeed": "500.0000", "COM9-StopBits": "2", "405 AO-Description": "NI DAC", "COM4-DelayBetweenCharsMs": "0.0000", "445 AO-Block voltage": "false", "520 AO-TriggerInputLine": "/Dev1/PFI0", "555 AO-Description": "NI DAC", "520 AO-TriggerSampleRate": "100000", "COM4-Verbose": "1", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM4-Name": "COM4", "405 Shutter-Volts": "0.0000", "Vincent-D3-Port": "COM1", "PRIME-PP 4 MIN ADU AFFECTED": "200", "470 Shutter-MinVolts": "0.0000", "PRIME-PP 3 ENABLED": "Yes", "470 AO-Description": "NI DAC", "PRIME-Description": "PVCAM API device adapter", "520 Shutter-MaxVolts": "5.0000", "520 Shutter-Volts": "0.0000", "528 AO-Sequenceable": "1", "PRIME-Binning": "1x1", "555 Shutter-MaxVolts": "5.0000", "528 Shutter-TriggerSequenceLength": "1000", "405 Shutter-Block voltage": "false", "XLIGHT Spin motor-State": "1", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "Vincent-D3-Time to open (ms)": "35.0000", "Vincent-D3-Time to close (ms)": "35.0000", "445 AO-TriggerSampleRate": "100000", "640 Shutter-IOChannel": "Dev1/ao14", "555 AO-Sequenceable": "1", "520 AO-Name": "AnalogIO", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "470 AO-Block voltage": "false", "COM1-Verbose": "1", "COM9-AnswerTimeout": "10000.0000", "528 Shutter-Name": "AnalogIO", "PRIME-Trigger Timeout (secs)": "10", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "NextFrame": 0, "470 AO-TriggerSequenceLength": "1000", "PRIME-TransposeXY": "0", "445 AO-TriggerSequenceLength": "1000", "COM1-Name": "COM1", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "COM9-DataBits": "8", "COM1-AnswerTimeout": "500.0000", "Camera": "", "TE2000-Version": "V2.50-V2.50-V2.10", "528 Shutter-Volts": "0.0000", "XLIGHT Hub-Name": "XLIGHT Hub", "PIEZO-Description": "Ludl stage driver adapter", "PositionName": "Pos17", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSampleRate": "100000", "PRIME-FanSpeedSetpoint": "High", "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "405 AO-TriggerSampleRate": "100000", "470 Shutter-Block voltage": "false", "470-State": "0", "COM3-Parity": "None", "405 AO-Volts": "0.0000", "PRIME-TransposeMirrorY": "0", "PRIME-ChipName": "GS144BSI", "470 AO-Volts": "0.0000", "PRIME-TransposeMirrorX": "0", "445 Shutter-SupportsTriggering": "0", "470 Shutter-SupportsTriggering": "0", "XLIGHT Hub-Description": "XLIGHT hub", "PRIME-PreampOffLimit": "10000", "470 AO-Sequenceable": "1", "528 Shutter-MaxVolts": "5.0000", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "Core-XYStage": "XYStage", "470 AO-TriggerSampleRate": "100000", "PRIME-Timing-PreTriggerDelayNs": "24672000.0000", "528-DA Device": "528 Shutter", "Focus-Description": "Nikon TE2000 focus adapter", "FilterBlock-State": "5", "520 AO-Demo": "No", "COM3-DelayBetweenCharsMs": "0.0000", "470 Shutter-Volts": "0.0000", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "PRIME-PP 2 THRESHOLD": "125", "PRIME-PP 4 THRESHOLD": "75", "XYStage-Acceleration": "4.0000", "470 Shutter-Demo": "No", "PRIME-Name": "PMPCIECam00", "640-DA Device": "640 Shutter", "405 AO-Block voltage": "false", "ElapsedTime-ms": 744538, "PRIME-Offset": "100", "445 AO-IOChannel": "Dev1/ao1", "640 AO-MaxVolts": "5.0000", "PositionIndex": 17, "PRIME-PP 1 ENABLED": "Yes", "COM1-BaudRate": "9600", "640-State": "0", "COM3-Verbose": "1", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "LudlController-TransmissionDelay": "0", "PRIME-FullWellCapacity": "62000", "PRIME-SMARTStreamingEnabled": "No", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "640 Shutter-Description": "NI DAC", "528-Description": "DA device that is used as a shutter", "COM4-Description": "Serial port driver (boost:asio)", "405 Shutter-Description": "NI DAC", "TE2000-Name": "TE2000", "528 Shutter-Description": "NI DAC", "COM9-Description": "Serial port driver (boost:asio)", "LudlController-Name": "LudlController", "445 Shutter-TriggerSequenceLength": "1000", "PRIME-SerialNumber": "A18B203008", "445 AO-Volts": "0.0000", "PRIME-X-dimension": "1200", "PRIME-ShutterCloseDelay": "0", "XLIGHT Disk slider-State": "2", "Position": "Default", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 AO-IOChannel": "Dev1/ao5", "520 AO-IOChannel": "Dev1/ao4", "528 AO-TriggerSequenceLength": "1000", "405 Shutter-MaxVolts": "5.0000", "470-DA Device": "470 Shutter", "PRIME-FTCapable": "No", "Core-ChannelGroup": "Channels", "555 AO-SupportsTriggering": "0", "PRIME-Y-dimension": "1200", "Frame": 0, "555 Shutter-Sequenceable": "1", "PRIME-Timing-ExposureTimeNs": "499998000.0000", "Core-ImageProcessor": "", "528-State": "0", "520 AO-Block voltage": "false", "XLIGHT Excitation Wheel-Label": "Excitation-2", "640 Shutter-Demo": "No", "COM3-StopBits": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "520 Shutter-IOChannel": "Dev1/ao11", "Width": 1200, "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-SupportsTriggering": "0", "640 AO-TriggerSampleRate": "100000", "520-Description": "DA device that is used as a shutter", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "405 Shutter-Sequenceable": "1", "405 Shutter-SupportsTriggering": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-TriggerSampleRate": "100000", "640 Shutter-MaxVolts": "5.0000", "555 AO-MaxVolts": "5.0000", "520 AO-TriggerSequenceLength": "1000", "COM3-BaudRate": "9600", "COM9-BaudRate": "9600", "Vincent-D3-Channel #": "Ch. #1", "PRIME-PMode": "Normal", "OpticalPath-Label": "Left(100)", "BitDepth": 16, "555 AO-TriggerSequenceLength": "1000", "445 Shutter-Description": "NI DAC", "405 AO-Sequenceable": "1", "PRIME-AcquisitionMethod": "Callbacks", "555 AO-Name": "AnalogIO", "PRIME-ShutterMode": "Pre-Exposure", "COM3-Handshaking": "Off", "445 AO-SupportsTriggering": "0", "ROI": "0-0-1200-1200", "COM9-Verbose": "1", "PRIME-Color": "OFF", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "PRIME-ShutterOpenDelay": "0", "COM1-Parity": "None", "Slice": 2, "SlicePosition": -30, "COM9-DelayBetweenCharsMs": "0.0000", "555-Name": "DA Shutter", "PIEZO-LudlSingleAxisName": "B", "XLIGHT Emission Wheel-Label": "Empty", "640 Shutter-Name": "AnalogIO", "640 AO-Sequenceable": "1", "520 Shutter-Demo": "No", "528 Shutter-Demo": "No", "470-Name": "DA Shutter", "520-DA Device": "520 Shutter", "XYStage-Description": "Ludl XY stage driver adapter", "445 Shutter-MinVolts": "0.0000", "528 AO-TriggerSampleRate": "100000", "405 AO-MinVolts": "0.0000", "405 Shutter-TriggerSequenceLength": "1000", "Height": 1200, "555 AO-Block voltage": "false", "640 Shutter-Volts": "0.0000", "PixelType": "GRAY16", "445 Shutter-Block voltage": "false", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "520 Shutter-TriggerSequenceLength": "1000", "COM9-Name": "COM9", "Core-SLM": "", "555 AO-Volts": "0.0000", "555 Shutter-Block voltage": "false", "XYStage-Speed": "1800.0000", "520 AO-MaxVolts": "5.0000", "528 Shutter-SupportsTriggering": "0", "COM1-Description": "Serial port driver (boost:asio)", "PRIME-MetadataEnabled": "No", "520 Shutter-MinVolts": "0.0000", "470 Shutter-Sequenceable": "1", "PRIME-PreampDelay": "0", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-MinVolts": "0.0000", "ChannelIndex": 4, "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Core-AutoShutter": "1", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "Exposure-ms": 20, "PRIME-PP 2 ENABLED": "Yes", "640 Shutter-TriggerSampleRate": "100000", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "470 Shutter-IOChannel": "Dev1/ao10", "405 AO-Demo": "No", "PixelSizeUm": 1.1, "470 AO-Demo": "No", "470 Shutter-MaxVolts": "5.0000", "Vincent-D3-Name": "Vincent-D3", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FileName": "img_000000000_P-DIC_002.tif", "LudlController-Reset": "Operate", "PRIME-Exposure": "20.00", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "445-Name": "DA Shutter", "555 Shutter-Name": "AnalogIO", "520 Shutter-TriggerSampleRate": "100000", "445 Shutter-IOChannel": "Dev1/ao9", "PRIME-TransposeCorrection": "0", "XYStage-StepSize": "0.1000", "445-Description": "DA device that is used as a shutter", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 AO-IOChannel": "Dev1/ao7", "520 Shutter-Name": "AnalogIO", "COM3-DataBits": "8", "640 AO-Volts": "0.0000", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "555 Shutter-IOChannel": "Dev1/ao13", "WaitInterval": null, "520 Shutter-Sequenceable": "1", "YPositionUm": -13012.300000000001, "445-State": "0", "445-DA Device": "445 Shutter", "PRIME-CircularBufferAutoSize": "ON", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "555 AO-TriggerSampleRate": "100000", "445 Shutter-Volts": "0.0000", "XLIGHT Hub-Port": "COM3", "Core-AutoFocus": "", "COM4-Handshaking": "Off", "528 Shutter-MinVolts": "0.0000", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "445 Shutter-Name": "AnalogIO", "528 Shutter-Sequenceable": "1", "XYStage-TransposeMirrorX": "0", "XYStage-TransposeMirrorY": "0", "PRIME-ReadoutRate": "100MHz 16bit", "XYStage-Name": "Stage", "640 AO-SupportsTriggering": "0", "640-Name": "DA Shutter", "640 Shutter-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "640 AO-Block voltage": "false", "445 Shutter-Sequenceable": "1", "470 AO-MaxVolts": "5.0000", "COM4-BaudRate": "9600", "470-Description": "DA device that is used as a shutter", "520 Shutter-Description": "NI DAC", "PRIME-CCDTemperature": "-14.9200", "Time": "2019-12-17 04:00:01 -0500", "640 AO-TriggerSequenceLength": "1000", "470 AO-Name": "AnalogIO", "XLIGHT Disk slider-Label": "Disk Pos 40um", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "Binning": "1x1", "TE2000-ModelType": "1", "COM3-AnswerTimeout": "500.0000", "555 Shutter-SupportsTriggering": "0", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "TE2000-Port": "COM4", "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Description": "NI DAC", "COM4-AnswerTimeout": "500.0000", "COM1-DelayBetweenCharsMs": "0.0000", "FilterBlock-Label": "Confocal", "445 AO-Name": "AnalogIO", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-Timing-ReadoutTimeNs": "24672000.0000", "528 AO-Demo": "No", "520 AO-Sequenceable": "1", "555-Description": "DA device that is used as a shutter", "405-DA Device": "405 Shutter", "405 AO-TriggerSequenceLength": "1000", "640 AO-MinVolts": "0.0000", "640 Shutter-Block voltage": "false", "FrameIndex": 0, "PRIME-Port": "CMOS", "555 AO-MinVolts": "0.0000", "COM3-Description": "Serial port driver (boost:asio)", "528 AO-Description": "NI DAC", "555 AO-IOChannel": "Dev1/ao6", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "555 AO-TriggerInputLine": "/Dev1/PFI0", "PRIME-ActualInterval-ms": "84.6200", "LudlController-Port": "COM9", "445 Shutter-Demo": "No", "PRIME-Trigger-Expose Out-Mux": "1", "SliceIndex": 2, "PRIME-CCDTemperatureSetPoint": "-15.0000", "405 Shutter-MinVolts": "0.0000", "PRIME-ExposeOutMode": "First Row", "AxisPositions": null, "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Volts": "0.0000", "528-Name": "DA Shutter", "555-State": "0", "XLIGHT Dichroic-Label": "Di03-R635-t1", "445 AO-Sequenceable": "1", "405-Name": "DA Shutter", "405 AO-IOChannel": "Dev1/ao0", "Core-Camera": "PRIME", "Focus-Name": "Focus", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "405 Shutter-IOChannel": "Dev1/ao8", "640 AO-Demo": "No", "640 Shutter-SupportsTriggering": "0", "445 AO-Demo": "No", "555-DA Device": "555 Shutter", "470 Shutter-Description": "NI DAC", "528 AO-Volts": "0.0000", "COM1-DataBits": "8", "Vincent-D3-Address": "x", "520 AO-SupportsTriggering": "0", "405-Description": "DA device that is used as a shutter", "640 Shutter-MinVolts": "0.0000", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "405 AO-Name": "AnalogIO", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "640 AO-TriggerInputLine": "/Dev1/PFI0", "405 AO-TriggerInputLine": "/Dev1/PFI0", "640-Description": "DA device that is used as a shutter", "445 Shutter-TriggerSampleRate": "100000", "470 Shutter-TriggerSequenceLength": "1000", "COM1-StopBits": "1", "520 Shutter-SupportsTriggering": "0", "405-State": "0", "Core-Initialize": "1", "XLIGHT Emission Wheel-State": "3", "520 AO-Description": "NI DAC", "528 AO-TriggerInputLine": "/Dev1/PFI0", "445 Shutter-MaxVolts": "5.0000", "COM9-Handshaking": "Off", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "XLIGHT Spin motor-Label": "On (spinning)", "PRIME-PP 5 ENABLED": "No", "UUID": "208e1433-9248-4442-820d-9cdb342440c7", "PRIME-TriggerMode": "Internal Trigger", "COM1-Handshaking": "Off", "PRIME-Timing-PostTriggerDelayNs": "49344000.0000", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "Focus-Position": "16732.9000", "528 Shutter-Block voltage": "false", "Channel": "P-DIC", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "555 Shutter-Demo": "No", "405 Shutter-Demo": "No", "528 AO-Name": "AnalogIO", "520-State": "0", "Core-Galvo": "", "405 Shutter-TriggerSampleRate": "100000", "Focus-FocusStepSize_(nm)": "100", "PRIME-PixelType": "16bit", "COM4-DataBits": "8", "470 AO-IOChannel": "Dev1/ao2", "520 AO-MinVolts": "0.0000", "COM9-Parity": "None", "PRIME-CircularBufferEnabled": "ON", "PRIME-PP 6 Reset": "No", "640 AO-Description": "NI DAC", "Source": "PRIME", "445 AO-MinVolts": "0.0000", "PIEZO-Name": "Stage", "445 AO-Description": "NI DAC", "PRIME-CircularBufferFrameCount": "46", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "520 AO-Volts": "0.0000", "528 Shutter-TriggerSampleRate": "100000", "XPositionUm": 23269.100000000002, "OpticalPath-State": "4", "PRIME-PP 3 THRESHOLD": "80", "PRIME-PVCAM Version": "3.7.1", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Gain": "1-HDR", "640 Shutter-TriggerSequenceLength": "1000", "528 AO-SupportsTriggering": "0", "445 AO-TriggerInputLine": "/Dev1/PFI0", "OpticalPath-Name": "OpticalPath", "PRIME-PP 4 ENABLED": "Yes", "PRIME-PVCAM Adapter Version": "1.3.29", "COM3-Name": "COM3", "405 AO-MaxVolts": "5.0000", "520 Shutter-Block voltage": "false", "PRIME-Timing-ClearingTimeNs": "49344000.0000", "Core-Focus": "PIEZO", "528 AO-Block voltage": "false", "TE2000-Description": "Nikon TE2000 hub", "528 AO-MinVolts": "0.0000", "COM4-Parity": "None", "Summary": { "Slices": 11, "ChContrastMin": [ 0, 0, 0, 0, 0, 0 ], "PixelAspect": 1, "SlicesFirst": true, "Interval_ms": 0, "Time": "2019-12-17 03:59:33 -0500", "Prefix": "seq_7", "InitialPositionList": [ { "Label": "Pos0", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [149.91], "Focus": [16657.7], "XYStage": [ 71629.7, 19152.9 ] }, "GridColumnIndex": 0 }, { "Label": "Pos1", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [190.82], "Focus": [16485.4], "XYStage": [ 65702.3, 19396.600000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos2", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [194.69], "Focus": [16446.8], "XYStage": [ 59880.5, 19007.8 ] }, "GridColumnIndex": 0 }, { "Label": "Pos3", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [145.04], "Focus": [16446.6], "XYStage": [ 53616.9, 18818.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos4", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [169.57500000000002], "Focus": [16446.5], "XYStage": [ 47662.8, 18817.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos5", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [131.805], "Focus": [16446.4], "XYStage": [ 41918.8, 18298.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos6", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [113.145], "Focus": [16446.5], "XYStage": [ 36129.9, 18895.8 ] }, "GridColumnIndex": 0 }, { "Label": "Pos7", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [141.005], "Focus": [16446.6], "XYStage": [ 30299.100000000002, 18895.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos8", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [140.97], "Focus": [16446.5], "XYStage": [ 23952.100000000002, 19156 ] }, "GridColumnIndex": 0 }, { "Label": "Pos9", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [184.57500000000002], "Focus": [16677.9], "XYStage": [ 72866.8, -14809.300000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos10", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [167.95000000000002], "Focus": [16557.9], "XYStage": [ 66515.6, -14371.300000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos11", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [168], "Focus": [16618.9], "XYStage": [ 59724.3, -13964.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos12", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [203.205], "Focus": [16779.6], "XYStage": [ 53862.3, -13494.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos13", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [203.20000000000002], "Focus": [16749], "XYStage": [ 48108.3, -13805.400000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos14", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [203.19], "Focus": [16675.9], "XYStage": [ 41645, -13804.900000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos15", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [203.21], "Focus": [16675.7], "XYStage": [ 35352.6, -13805.6 ] }, "GridColumnIndex": 0 }, { "Label": "Pos16", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [203.225], "Focus": [16675.8], "XYStage": [ 29298, -13447.400000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos17", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [203.215], "Focus": [16675.8], "XYStage": [ 23269.100000000002, -13012.300000000001 ] }, "GridColumnIndex": 0 } ], "z-step_um": 10, "GridRow": 0, "BitDepth": 16, "Depth": 2, "Channels": 6, "Source": "Micro-Manager", "ChColors": [ -16711681, -256, -65281, -1, -1, -16776961 ], "MetadataVersion": 10, "KeepShutterOpenChannels": true, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536, 65536 ], "CustomIntervals_ms": [], "Height": 1200, "MicroManagerVersion": "1.4.23 20180219", "GridColumn": 0, "UUID": "3ad8801b-5699-40d6-813b-4ad840863e38", "PixelType": "GRAY16", "Width": 1200, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\Yan\\20191216-YC113-S11S12-miseq", "Frames": 1, "Comment": "", "UserName": "Nikon", "PixelSize_um": 1.1, "ROI": [ 0, 0, 1200, 1200 ], "Date": "2019-12-17", "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC", "P-DAPI-high" ], "KeepShutterOpenSlices": true, "IJType": 1, "ComputerName": "ZadorConfocal2", "Positions": 18, "PositionIndex": 17, "CameraTimeout": 20000, "TimeFirst": false }, "640 AO-Name": "AnalogIO", "Vincent-D3-Command": "Close" }, "FrameKey-0-4-3": { "COM4-StopBits": "1", "528 AO-MaxVolts": "5.0000", "XLIGHT Excitation Wheel-State": "1", "470 Shutter-Name": "AnalogIO", "Core-Shutter": "Vincent-D3", "555 AO-Demo": "No", "XLIGHT Dichroic-State": "2", "XYStage-StepSize-Y": "0.1000", "ZPositionUm": 183.215, "XYStage-StepSize-X": "0.1000", "PRIME-FirmwareVersion": "20.28", "PRIME-ClearCycles": "2", "520-Name": "DA Shutter", "405 Shutter-Name": "AnalogIO", "PIEZO-StepSize": "0.0050", "PRIME-ClearMode": "Pre-Exposure", "470 AO-MinVolts": "0.0000", "405 AO-SupportsTriggering": "1", "CameraChannelIndex": 0, "XYStage-StartSpeed": "500.0000", "COM9-StopBits": "2", "405 AO-Description": "NI DAC", "COM4-DelayBetweenCharsMs": "0.0000", "445 AO-Block voltage": "false", "520 AO-TriggerInputLine": "/Dev1/PFI0", "555 AO-Description": "NI DAC", "520 AO-TriggerSampleRate": "100000", "COM4-Verbose": "1", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM4-Name": "COM4", "405 Shutter-Volts": "0.0000", "Vincent-D3-Port": "COM1", "PRIME-PP 4 MIN ADU AFFECTED": "200", "470 Shutter-MinVolts": "0.0000", "PRIME-PP 3 ENABLED": "Yes", "470 AO-Description": "NI DAC", "PRIME-Description": "PVCAM API device adapter", "520 Shutter-MaxVolts": "5.0000", "520 Shutter-Volts": "0.0000", "528 AO-Sequenceable": "1", "PRIME-Binning": "1x1", "555 Shutter-MaxVolts": "5.0000", "528 Shutter-TriggerSequenceLength": "1000", "405 Shutter-Block voltage": "false", "XLIGHT Spin motor-State": "1", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "Vincent-D3-Time to open (ms)": "35.0000", "Vincent-D3-Time to close (ms)": "35.0000", "445 AO-TriggerSampleRate": "100000", "640 Shutter-IOChannel": "Dev1/ao14", "555 AO-Sequenceable": "1", "520 AO-Name": "AnalogIO", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "470 AO-Block voltage": "false", "COM1-Verbose": "1", "COM9-AnswerTimeout": "10000.0000", "528 Shutter-Name": "AnalogIO", "PRIME-Trigger Timeout (secs)": "10", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "NextFrame": 0, "470 AO-TriggerSequenceLength": "1000", "PRIME-TransposeXY": "0", "445 AO-TriggerSequenceLength": "1000", "COM1-Name": "COM1", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "COM9-DataBits": "8", "COM1-AnswerTimeout": "500.0000", "Camera": "", "TE2000-Version": "V2.50-V2.50-V2.10", "528 Shutter-Volts": "0.0000", "XLIGHT Hub-Name": "XLIGHT Hub", "PIEZO-Description": "Ludl stage driver adapter", "PositionName": "Pos17", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSampleRate": "100000", "PRIME-FanSpeedSetpoint": "High", "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "405 AO-TriggerSampleRate": "100000", "470 Shutter-Block voltage": "false", "470-State": "0", "COM3-Parity": "None", "405 AO-Volts": "0.0000", "PRIME-TransposeMirrorY": "0", "PRIME-ChipName": "GS144BSI", "470 AO-Volts": "0.0000", "PRIME-TransposeMirrorX": "0", "445 Shutter-SupportsTriggering": "0", "470 Shutter-SupportsTriggering": "0", "XLIGHT Hub-Description": "XLIGHT hub", "PRIME-PreampOffLimit": "10000", "470 AO-Sequenceable": "1", "528 Shutter-MaxVolts": "5.0000", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "Core-XYStage": "XYStage", "470 AO-TriggerSampleRate": "100000", "PRIME-Timing-PreTriggerDelayNs": "24672000.0000", "528-DA Device": "528 Shutter", "Focus-Description": "Nikon TE2000 focus adapter", "FilterBlock-State": "5", "520 AO-Demo": "No", "COM3-DelayBetweenCharsMs": "0.0000", "470 Shutter-Volts": "0.0000", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "PRIME-PP 2 THRESHOLD": "125", "PRIME-PP 4 THRESHOLD": "75", "XYStage-Acceleration": "4.0000", "470 Shutter-Demo": "No", "PRIME-Name": "PMPCIECam00", "640-DA Device": "640 Shutter", "405 AO-Block voltage": "false", "ElapsedTime-ms": 744847, "PRIME-Offset": "100", "445 AO-IOChannel": "Dev1/ao1", "640 AO-MaxVolts": "5.0000", "PositionIndex": 17, "PRIME-PP 1 ENABLED": "Yes", "COM1-BaudRate": "9600", "640-State": "0", "COM3-Verbose": "1", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "LudlController-TransmissionDelay": "0", "PRIME-FullWellCapacity": "62000", "PRIME-SMARTStreamingEnabled": "No", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "640 Shutter-Description": "NI DAC", "528-Description": "DA device that is used as a shutter", "COM4-Description": "Serial port driver (boost:asio)", "405 Shutter-Description": "NI DAC", "TE2000-Name": "TE2000", "528 Shutter-Description": "NI DAC", "COM9-Description": "Serial port driver (boost:asio)", "LudlController-Name": "LudlController", "445 Shutter-TriggerSequenceLength": "1000", "PRIME-SerialNumber": "A18B203008", "445 AO-Volts": "0.0000", "PRIME-X-dimension": "1200", "PRIME-ShutterCloseDelay": "0", "XLIGHT Disk slider-State": "2", "Position": "Default", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 AO-IOChannel": "Dev1/ao5", "520 AO-IOChannel": "Dev1/ao4", "528 AO-TriggerSequenceLength": "1000", "405 Shutter-MaxVolts": "5.0000", "470-DA Device": "470 Shutter", "PRIME-FTCapable": "No", "Core-ChannelGroup": "Channels", "555 AO-SupportsTriggering": "0", "PRIME-Y-dimension": "1200", "Frame": 0, "555 Shutter-Sequenceable": "1", "PRIME-Timing-ExposureTimeNs": "499998000.0000", "Core-ImageProcessor": "", "528-State": "0", "520 AO-Block voltage": "false", "XLIGHT Excitation Wheel-Label": "Excitation-2", "640 Shutter-Demo": "No", "COM3-StopBits": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "520 Shutter-IOChannel": "Dev1/ao11", "Width": 1200, "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-SupportsTriggering": "0", "640 AO-TriggerSampleRate": "100000", "520-Description": "DA device that is used as a shutter", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "405 Shutter-Sequenceable": "1", "405 Shutter-SupportsTriggering": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-TriggerSampleRate": "100000", "640 Shutter-MaxVolts": "5.0000", "555 AO-MaxVolts": "5.0000", "520 AO-TriggerSequenceLength": "1000", "COM3-BaudRate": "9600", "COM9-BaudRate": "9600", "Vincent-D3-Channel #": "Ch. #1", "PRIME-PMode": "Normal", "OpticalPath-Label": "Left(100)", "BitDepth": 16, "555 AO-TriggerSequenceLength": "1000", "445 Shutter-Description": "NI DAC", "405 AO-Sequenceable": "1", "PRIME-AcquisitionMethod": "Callbacks", "555 AO-Name": "AnalogIO", "PRIME-ShutterMode": "Pre-Exposure", "COM3-Handshaking": "Off", "445 AO-SupportsTriggering": "0", "ROI": "0-0-1200-1200", "COM9-Verbose": "1", "PRIME-Color": "OFF", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "PRIME-ShutterOpenDelay": "0", "COM1-Parity": "None", "Slice": 3, "SlicePosition": -20, "COM9-DelayBetweenCharsMs": "0.0000", "555-Name": "DA Shutter", "PIEZO-LudlSingleAxisName": "B", "XLIGHT Emission Wheel-Label": "Empty", "640 Shutter-Name": "AnalogIO", "640 AO-Sequenceable": "1", "520 Shutter-Demo": "No", "528 Shutter-Demo": "No", "470-Name": "DA Shutter", "520-DA Device": "520 Shutter", "XYStage-Description": "Ludl XY stage driver adapter", "445 Shutter-MinVolts": "0.0000", "528 AO-TriggerSampleRate": "100000", "405 AO-MinVolts": "0.0000", "405 Shutter-TriggerSequenceLength": "1000", "Height": 1200, "555 AO-Block voltage": "false", "640 Shutter-Volts": "0.0000", "PixelType": "GRAY16", "445 Shutter-Block voltage": "false", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "520 Shutter-TriggerSequenceLength": "1000", "COM9-Name": "COM9", "Core-SLM": "", "555 AO-Volts": "0.0000", "555 Shutter-Block voltage": "false", "XYStage-Speed": "1800.0000", "520 AO-MaxVolts": "5.0000", "528 Shutter-SupportsTriggering": "0", "COM1-Description": "Serial port driver (boost:asio)", "PRIME-MetadataEnabled": "No", "520 Shutter-MinVolts": "0.0000", "470 Shutter-Sequenceable": "1", "PRIME-PreampDelay": "0", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-MinVolts": "0.0000", "ChannelIndex": 4, "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Core-AutoShutter": "1", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "Exposure-ms": 20, "PRIME-PP 2 ENABLED": "Yes", "640 Shutter-TriggerSampleRate": "100000", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "470 Shutter-IOChannel": "Dev1/ao10", "405 AO-Demo": "No", "PixelSizeUm": 1.1, "470 AO-Demo": "No", "470 Shutter-MaxVolts": "5.0000", "Vincent-D3-Name": "Vincent-D3", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FileName": "img_000000000_P-DIC_003.tif", "LudlController-Reset": "Operate", "PRIME-Exposure": "20.00", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "445-Name": "DA Shutter", "555 Shutter-Name": "AnalogIO", "520 Shutter-TriggerSampleRate": "100000", "445 Shutter-IOChannel": "Dev1/ao9", "PRIME-TransposeCorrection": "0", "XYStage-StepSize": "0.1000", "445-Description": "DA device that is used as a shutter", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 AO-IOChannel": "Dev1/ao7", "520 Shutter-Name": "AnalogIO", "COM3-DataBits": "8", "640 AO-Volts": "0.0000", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "555 Shutter-IOChannel": "Dev1/ao13", "WaitInterval": null, "520 Shutter-Sequenceable": "1", "YPositionUm": -13012.300000000001, "445-State": "0", "445-DA Device": "445 Shutter", "PRIME-CircularBufferAutoSize": "ON", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "555 AO-TriggerSampleRate": "100000", "445 Shutter-Volts": "0.0000", "XLIGHT Hub-Port": "COM3", "Core-AutoFocus": "", "COM4-Handshaking": "Off", "528 Shutter-MinVolts": "0.0000", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "445 Shutter-Name": "AnalogIO", "528 Shutter-Sequenceable": "1", "XYStage-TransposeMirrorX": "0", "XYStage-TransposeMirrorY": "0", "PRIME-ReadoutRate": "100MHz 16bit", "XYStage-Name": "Stage", "640 AO-SupportsTriggering": "0", "640-Name": "DA Shutter", "640 Shutter-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "640 AO-Block voltage": "false", "445 Shutter-Sequenceable": "1", "470 AO-MaxVolts": "5.0000", "COM4-BaudRate": "9600", "470-Description": "DA device that is used as a shutter", "520 Shutter-Description": "NI DAC", "PRIME-CCDTemperature": "-14.9200", "Time": "2019-12-17 04:00:01 -0500", "640 AO-TriggerSequenceLength": "1000", "470 AO-Name": "AnalogIO", "XLIGHT Disk slider-Label": "Disk Pos 40um", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "Binning": "1x1", "TE2000-ModelType": "1", "COM3-AnswerTimeout": "500.0000", "555 Shutter-SupportsTriggering": "0", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "TE2000-Port": "COM4", "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Description": "NI DAC", "COM4-AnswerTimeout": "500.0000", "COM1-DelayBetweenCharsMs": "0.0000", "FilterBlock-Label": "Confocal", "445 AO-Name": "AnalogIO", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-Timing-ReadoutTimeNs": "24672000.0000", "528 AO-Demo": "No", "520 AO-Sequenceable": "1", "555-Description": "DA device that is used as a shutter", "405-DA Device": "405 Shutter", "405 AO-TriggerSequenceLength": "1000", "640 AO-MinVolts": "0.0000", "640 Shutter-Block voltage": "false", "FrameIndex": 0, "PRIME-Port": "CMOS", "555 AO-MinVolts": "0.0000", "COM3-Description": "Serial port driver (boost:asio)", "528 AO-Description": "NI DAC", "555 AO-IOChannel": "Dev1/ao6", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "555 AO-TriggerInputLine": "/Dev1/PFI0", "PRIME-ActualInterval-ms": "84.6200", "LudlController-Port": "COM9", "445 Shutter-Demo": "No", "PRIME-Trigger-Expose Out-Mux": "1", "SliceIndex": 3, "PRIME-CCDTemperatureSetPoint": "-15.0000", "405 Shutter-MinVolts": "0.0000", "PRIME-ExposeOutMode": "First Row", "AxisPositions": null, "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Volts": "0.0000", "528-Name": "DA Shutter", "555-State": "0", "XLIGHT Dichroic-Label": "Di03-R635-t1", "445 AO-Sequenceable": "1", "405-Name": "DA Shutter", "405 AO-IOChannel": "Dev1/ao0", "Core-Camera": "PRIME", "Focus-Name": "Focus", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "405 Shutter-IOChannel": "Dev1/ao8", "640 AO-Demo": "No", "640 Shutter-SupportsTriggering": "0", "445 AO-Demo": "No", "555-DA Device": "555 Shutter", "470 Shutter-Description": "NI DAC", "528 AO-Volts": "0.0000", "COM1-DataBits": "8", "Vincent-D3-Address": "x", "520 AO-SupportsTriggering": "0", "405-Description": "DA device that is used as a shutter", "640 Shutter-MinVolts": "0.0000", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "405 AO-Name": "AnalogIO", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "640 AO-TriggerInputLine": "/Dev1/PFI0", "405 AO-TriggerInputLine": "/Dev1/PFI0", "640-Description": "DA device that is used as a shutter", "445 Shutter-TriggerSampleRate": "100000", "470 Shutter-TriggerSequenceLength": "1000", "COM1-StopBits": "1", "520 Shutter-SupportsTriggering": "0", "405-State": "0", "Core-Initialize": "1", "XLIGHT Emission Wheel-State": "3", "520 AO-Description": "NI DAC", "528 AO-TriggerInputLine": "/Dev1/PFI0", "445 Shutter-MaxVolts": "5.0000", "COM9-Handshaking": "Off", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "XLIGHT Spin motor-Label": "On (spinning)", "PRIME-PP 5 ENABLED": "No", "UUID": "d0f5c6e2-c54a-4e21-ae54-aae496d2277b", "PRIME-TriggerMode": "Internal Trigger", "COM1-Handshaking": "Off", "PRIME-Timing-PostTriggerDelayNs": "49344000.0000", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "Focus-Position": "16732.9000", "528 Shutter-Block voltage": "false", "Channel": "P-DIC", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "555 Shutter-Demo": "No", "405 Shutter-Demo": "No", "528 AO-Name": "AnalogIO", "520-State": "0", "Core-Galvo": "", "405 Shutter-TriggerSampleRate": "100000", "Focus-FocusStepSize_(nm)": "100", "PRIME-PixelType": "16bit", "COM4-DataBits": "8", "470 AO-IOChannel": "Dev1/ao2", "520 AO-MinVolts": "0.0000", "COM9-Parity": "None", "PRIME-CircularBufferEnabled": "ON", "PRIME-PP 6 Reset": "No", "640 AO-Description": "NI DAC", "Source": "PRIME", "445 AO-MinVolts": "0.0000", "PIEZO-Name": "Stage", "445 AO-Description": "NI DAC", "PRIME-CircularBufferFrameCount": "46", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "520 AO-Volts": "0.0000", "528 Shutter-TriggerSampleRate": "100000", "XPositionUm": 23269.100000000002, "OpticalPath-State": "4", "PRIME-PP 3 THRESHOLD": "80", "PRIME-PVCAM Version": "3.7.1", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Gain": "1-HDR", "640 Shutter-TriggerSequenceLength": "1000", "528 AO-SupportsTriggering": "0", "445 AO-TriggerInputLine": "/Dev1/PFI0", "OpticalPath-Name": "OpticalPath", "PRIME-PP 4 ENABLED": "Yes", "PRIME-PVCAM Adapter Version": "1.3.29", "COM3-Name": "COM3", "405 AO-MaxVolts": "5.0000", "520 Shutter-Block voltage": "false", "PRIME-Timing-ClearingTimeNs": "49344000.0000", "Core-Focus": "PIEZO", "528 AO-Block voltage": "false", "TE2000-Description": "Nikon TE2000 hub", "528 AO-MinVolts": "0.0000", "COM4-Parity": "None", "Summary": { "Slices": 11, "ChContrastMin": [ 0, 0, 0, 0, 0, 0 ], "PixelAspect": 1, "SlicesFirst": true, "Interval_ms": 0, "Time": "2019-12-17 03:59:33 -0500", "Prefix": "seq_7", "InitialPositionList": [ { "Label": "Pos0", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [149.91], "Focus": [16657.7], "XYStage": [ 71629.7, 19152.9 ] }, "GridColumnIndex": 0 }, { "Label": "Pos1", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [190.82], "Focus": [16485.4], "XYStage": [ 65702.3, 19396.600000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos2", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [194.69], "Focus": [16446.8], "XYStage": [ 59880.5, 19007.8 ] }, "GridColumnIndex": 0 }, { "Label": "Pos3", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [145.04], "Focus": [16446.6], "XYStage": [ 53616.9, 18818.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos4", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [169.57500000000002], "Focus": [16446.5], "XYStage": [ 47662.8, 18817.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos5", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [131.805], "Focus": [16446.4], "XYStage": [ 41918.8, 18298.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos6", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [113.145], "Focus": [16446.5], "XYStage": [ 36129.9, 18895.8 ] }, "GridColumnIndex": 0 }, { "Label": "Pos7", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [141.005], "Focus": [16446.6], "XYStage": [ 30299.100000000002, 18895.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos8", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [140.97], "Focus": [16446.5], "XYStage": [ 23952.100000000002, 19156 ] }, "GridColumnIndex": 0 }, { "Label": "Pos9", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [184.57500000000002], "Focus": [16677.9], "XYStage": [ 72866.8, -14809.300000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos10", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [167.95000000000002], "Focus": [16557.9], "XYStage": [ 66515.6, -14371.300000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos11", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [168], "Focus": [16618.9], "XYStage": [ 59724.3, -13964.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos12", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [203.205], "Focus": [16779.6], "XYStage": [ 53862.3, -13494.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos13", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [203.20000000000002], "Focus": [16749], "XYStage": [ 48108.3, -13805.400000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos14", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [203.19], "Focus": [16675.9], "XYStage": [ 41645, -13804.900000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos15", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [203.21], "Focus": [16675.7], "XYStage": [ 35352.6, -13805.6 ] }, "GridColumnIndex": 0 }, { "Label": "Pos16", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [203.225], "Focus": [16675.8], "XYStage": [ 29298, -13447.400000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos17", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [203.215], "Focus": [16675.8], "XYStage": [ 23269.100000000002, -13012.300000000001 ] }, "GridColumnIndex": 0 } ], "z-step_um": 10, "GridRow": 0, "BitDepth": 16, "Depth": 2, "Channels": 6, "Source": "Micro-Manager", "ChColors": [ -16711681, -256, -65281, -1, -1, -16776961 ], "MetadataVersion": 10, "KeepShutterOpenChannels": true, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536, 65536 ], "CustomIntervals_ms": [], "Height": 1200, "MicroManagerVersion": "1.4.23 20180219", "GridColumn": 0, "UUID": "3ad8801b-5699-40d6-813b-4ad840863e38", "PixelType": "GRAY16", "Width": 1200, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\Yan\\20191216-YC113-S11S12-miseq", "Frames": 1, "Comment": "", "UserName": "Nikon", "PixelSize_um": 1.1, "ROI": [ 0, 0, 1200, 1200 ], "Date": "2019-12-17", "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC", "P-DAPI-high" ], "KeepShutterOpenSlices": true, "IJType": 1, "ComputerName": "ZadorConfocal2", "Positions": 18, "PositionIndex": 17, "CameraTimeout": 20000, "TimeFirst": false }, "640 AO-Name": "AnalogIO", "Vincent-D3-Command": "Close" }, "FrameKey-0-4-4": { "COM4-StopBits": "1", "528 AO-MaxVolts": "5.0000", "XLIGHT Excitation Wheel-State": "1", "470 Shutter-Name": "AnalogIO", "Core-Shutter": "Vincent-D3", "555 AO-Demo": "No", "XLIGHT Dichroic-State": "2", "XYStage-StepSize-Y": "0.1000", "ZPositionUm": 193.215, "XYStage-StepSize-X": "0.1000", "PRIME-FirmwareVersion": "20.28", "PRIME-ClearCycles": "2", "520-Name": "DA Shutter", "405 Shutter-Name": "AnalogIO", "PIEZO-StepSize": "0.0050", "PRIME-ClearMode": "Pre-Exposure", "470 AO-MinVolts": "0.0000", "405 AO-SupportsTriggering": "1", "CameraChannelIndex": 0, "XYStage-StartSpeed": "500.0000", "COM9-StopBits": "2", "405 AO-Description": "NI DAC", "COM4-DelayBetweenCharsMs": "0.0000", "445 AO-Block voltage": "false", "520 AO-TriggerInputLine": "/Dev1/PFI0", "555 AO-Description": "NI DAC", "520 AO-TriggerSampleRate": "100000", "COM4-Verbose": "1", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM4-Name": "COM4", "405 Shutter-Volts": "0.0000", "Vincent-D3-Port": "COM1", "PRIME-PP 4 MIN ADU AFFECTED": "200", "470 Shutter-MinVolts": "0.0000", "PRIME-PP 3 ENABLED": "Yes", "470 AO-Description": "NI DAC", "PRIME-Description": "PVCAM API device adapter", "520 Shutter-MaxVolts": "5.0000", "520 Shutter-Volts": "0.0000", "528 AO-Sequenceable": "1", "PRIME-Binning": "1x1", "555 Shutter-MaxVolts": "5.0000", "528 Shutter-TriggerSequenceLength": "1000", "405 Shutter-Block voltage": "false", "XLIGHT Spin motor-State": "1", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "Vincent-D3-Time to open (ms)": "35.0000", "Vincent-D3-Time to close (ms)": "35.0000", "445 AO-TriggerSampleRate": "100000", "640 Shutter-IOChannel": "Dev1/ao14", "555 AO-Sequenceable": "1", "520 AO-Name": "AnalogIO", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "470 AO-Block voltage": "false", "COM1-Verbose": "1", "COM9-AnswerTimeout": "10000.0000", "528 Shutter-Name": "AnalogIO", "PRIME-Trigger Timeout (secs)": "10", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "NextFrame": 0, "470 AO-TriggerSequenceLength": "1000", "PRIME-TransposeXY": "0", "445 AO-TriggerSequenceLength": "1000", "COM1-Name": "COM1", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "COM9-DataBits": "8", "COM1-AnswerTimeout": "500.0000", "Camera": "", "TE2000-Version": "V2.50-V2.50-V2.10", "528 Shutter-Volts": "0.0000", "XLIGHT Hub-Name": "XLIGHT Hub", "PIEZO-Description": "Ludl stage driver adapter", "PositionName": "Pos17", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSampleRate": "100000", "PRIME-FanSpeedSetpoint": "High", "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "405 AO-TriggerSampleRate": "100000", "470 Shutter-Block voltage": "false", "470-State": "0", "COM3-Parity": "None", "405 AO-Volts": "0.0000", "PRIME-TransposeMirrorY": "0", "PRIME-ChipName": "GS144BSI", "470 AO-Volts": "0.0000", "PRIME-TransposeMirrorX": "0", "445 Shutter-SupportsTriggering": "0", "470 Shutter-SupportsTriggering": "0", "XLIGHT Hub-Description": "XLIGHT hub", "PRIME-PreampOffLimit": "10000", "470 AO-Sequenceable": "1", "528 Shutter-MaxVolts": "5.0000", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "Core-XYStage": "XYStage", "470 AO-TriggerSampleRate": "100000", "PRIME-Timing-PreTriggerDelayNs": "24672000.0000", "528-DA Device": "528 Shutter", "Focus-Description": "Nikon TE2000 focus adapter", "FilterBlock-State": "5", "520 AO-Demo": "No", "COM3-DelayBetweenCharsMs": "0.0000", "470 Shutter-Volts": "0.0000", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "PRIME-PP 2 THRESHOLD": "125", "PRIME-PP 4 THRESHOLD": "75", "XYStage-Acceleration": "4.0000", "470 Shutter-Demo": "No", "PRIME-Name": "PMPCIECam00", "640-DA Device": "640 Shutter", "405 AO-Block voltage": "false", "ElapsedTime-ms": 745114, "PRIME-Offset": "100", "445 AO-IOChannel": "Dev1/ao1", "640 AO-MaxVolts": "5.0000", "PositionIndex": 17, "PRIME-PP 1 ENABLED": "Yes", "COM1-BaudRate": "9600", "640-State": "0", "COM3-Verbose": "1", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "LudlController-TransmissionDelay": "0", "PRIME-FullWellCapacity": "62000", "PRIME-SMARTStreamingEnabled": "No", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "640 Shutter-Description": "NI DAC", "528-Description": "DA device that is used as a shutter", "COM4-Description": "Serial port driver (boost:asio)", "405 Shutter-Description": "NI DAC", "TE2000-Name": "TE2000", "528 Shutter-Description": "NI DAC", "COM9-Description": "Serial port driver (boost:asio)", "LudlController-Name": "LudlController", "445 Shutter-TriggerSequenceLength": "1000", "PRIME-SerialNumber": "A18B203008", "445 AO-Volts": "0.0000", "PRIME-X-dimension": "1200", "PRIME-ShutterCloseDelay": "0", "XLIGHT Disk slider-State": "2", "Position": "Default", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 AO-IOChannel": "Dev1/ao5", "520 AO-IOChannel": "Dev1/ao4", "528 AO-TriggerSequenceLength": "1000", "405 Shutter-MaxVolts": "5.0000", "470-DA Device": "470 Shutter", "PRIME-FTCapable": "No", "Core-ChannelGroup": "Channels", "555 AO-SupportsTriggering": "0", "PRIME-Y-dimension": "1200", "Frame": 0, "555 Shutter-Sequenceable": "1", "PRIME-Timing-ExposureTimeNs": "499998000.0000", "Core-ImageProcessor": "", "528-State": "0", "520 AO-Block voltage": "false", "XLIGHT Excitation Wheel-Label": "Excitation-2", "640 Shutter-Demo": "No", "COM3-StopBits": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "520 Shutter-IOChannel": "Dev1/ao11", "Width": 1200, "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-SupportsTriggering": "0", "640 AO-TriggerSampleRate": "100000", "520-Description": "DA device that is used as a shutter", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "405 Shutter-Sequenceable": "1", "405 Shutter-SupportsTriggering": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-TriggerSampleRate": "100000", "640 Shutter-MaxVolts": "5.0000", "555 AO-MaxVolts": "5.0000", "520 AO-TriggerSequenceLength": "1000", "COM3-BaudRate": "9600", "COM9-BaudRate": "9600", "Vincent-D3-Channel #": "Ch. #1", "PRIME-PMode": "Normal", "OpticalPath-Label": "Left(100)", "BitDepth": 16, "555 AO-TriggerSequenceLength": "1000", "445 Shutter-Description": "NI DAC", "405 AO-Sequenceable": "1", "PRIME-AcquisitionMethod": "Callbacks", "555 AO-Name": "AnalogIO", "PRIME-ShutterMode": "Pre-Exposure", "COM3-Handshaking": "Off", "445 AO-SupportsTriggering": "0", "ROI": "0-0-1200-1200", "COM9-Verbose": "1", "PRIME-Color": "OFF", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "PRIME-ShutterOpenDelay": "0", "COM1-Parity": "None", "Slice": 4, "SlicePosition": -10, "COM9-DelayBetweenCharsMs": "0.0000", "555-Name": "DA Shutter", "PIEZO-LudlSingleAxisName": "B", "XLIGHT Emission Wheel-Label": "Empty", "640 Shutter-Name": "AnalogIO", "640 AO-Sequenceable": "1", "520 Shutter-Demo": "No", "528 Shutter-Demo": "No", "470-Name": "DA Shutter", "520-DA Device": "520 Shutter", "XYStage-Description": "Ludl XY stage driver adapter", "445 Shutter-MinVolts": "0.0000", "528 AO-TriggerSampleRate": "100000", "405 AO-MinVolts": "0.0000", "405 Shutter-TriggerSequenceLength": "1000", "Height": 1200, "555 AO-Block voltage": "false", "640 Shutter-Volts": "0.0000", "PixelType": "GRAY16", "445 Shutter-Block voltage": "false", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "520 Shutter-TriggerSequenceLength": "1000", "COM9-Name": "COM9", "Core-SLM": "", "555 AO-Volts": "0.0000", "555 Shutter-Block voltage": "false", "XYStage-Speed": "1800.0000", "520 AO-MaxVolts": "5.0000", "528 Shutter-SupportsTriggering": "0", "COM1-Description": "Serial port driver (boost:asio)", "PRIME-MetadataEnabled": "No", "520 Shutter-MinVolts": "0.0000", "470 Shutter-Sequenceable": "1", "PRIME-PreampDelay": "0", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-MinVolts": "0.0000", "ChannelIndex": 4, "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Core-AutoShutter": "1", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "Exposure-ms": 20, "PRIME-PP 2 ENABLED": "Yes", "640 Shutter-TriggerSampleRate": "100000", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "470 Shutter-IOChannel": "Dev1/ao10", "405 AO-Demo": "No", "PixelSizeUm": 1.1, "470 AO-Demo": "No", "470 Shutter-MaxVolts": "5.0000", "Vincent-D3-Name": "Vincent-D3", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FileName": "img_000000000_P-DIC_004.tif", "LudlController-Reset": "Operate", "PRIME-Exposure": "20.00", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "445-Name": "DA Shutter", "555 Shutter-Name": "AnalogIO", "520 Shutter-TriggerSampleRate": "100000", "445 Shutter-IOChannel": "Dev1/ao9", "PRIME-TransposeCorrection": "0", "XYStage-StepSize": "0.1000", "445-Description": "DA device that is used as a shutter", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 AO-IOChannel": "Dev1/ao7", "520 Shutter-Name": "AnalogIO", "COM3-DataBits": "8", "640 AO-Volts": "0.0000", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "555 Shutter-IOChannel": "Dev1/ao13", "WaitInterval": null, "520 Shutter-Sequenceable": "1", "YPositionUm": -13012.300000000001, "445-State": "0", "445-DA Device": "445 Shutter", "PRIME-CircularBufferAutoSize": "ON", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "555 AO-TriggerSampleRate": "100000", "445 Shutter-Volts": "0.0000", "XLIGHT Hub-Port": "COM3", "Core-AutoFocus": "", "COM4-Handshaking": "Off", "528 Shutter-MinVolts": "0.0000", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "445 Shutter-Name": "AnalogIO", "528 Shutter-Sequenceable": "1", "XYStage-TransposeMirrorX": "0", "XYStage-TransposeMirrorY": "0", "PRIME-ReadoutRate": "100MHz 16bit", "XYStage-Name": "Stage", "640 AO-SupportsTriggering": "0", "640-Name": "DA Shutter", "640 Shutter-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "640 AO-Block voltage": "false", "445 Shutter-Sequenceable": "1", "470 AO-MaxVolts": "5.0000", "COM4-BaudRate": "9600", "470-Description": "DA device that is used as a shutter", "520 Shutter-Description": "NI DAC", "PRIME-CCDTemperature": "-14.9200", "Time": "2019-12-17 04:00:02 -0500", "640 AO-TriggerSequenceLength": "1000", "470 AO-Name": "AnalogIO", "XLIGHT Disk slider-Label": "Disk Pos 40um", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "Binning": "1x1", "TE2000-ModelType": "1", "COM3-AnswerTimeout": "500.0000", "555 Shutter-SupportsTriggering": "0", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "TE2000-Port": "COM4", "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Description": "NI DAC", "COM4-AnswerTimeout": "500.0000", "COM1-DelayBetweenCharsMs": "0.0000", "FilterBlock-Label": "Confocal", "445 AO-Name": "AnalogIO", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-Timing-ReadoutTimeNs": "24672000.0000", "528 AO-Demo": "No", "520 AO-Sequenceable": "1", "555-Description": "DA device that is used as a shutter", "405-DA Device": "405 Shutter", "405 AO-TriggerSequenceLength": "1000", "640 AO-MinVolts": "0.0000", "640 Shutter-Block voltage": "false", "FrameIndex": 0, "PRIME-Port": "CMOS", "555 AO-MinVolts": "0.0000", "COM3-Description": "Serial port driver (boost:asio)", "528 AO-Description": "NI DAC", "555 AO-IOChannel": "Dev1/ao6", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "555 AO-TriggerInputLine": "/Dev1/PFI0", "PRIME-ActualInterval-ms": "84.6200", "LudlController-Port": "COM9", "445 Shutter-Demo": "No", "PRIME-Trigger-Expose Out-Mux": "1", "SliceIndex": 4, "PRIME-CCDTemperatureSetPoint": "-15.0000", "405 Shutter-MinVolts": "0.0000", "PRIME-ExposeOutMode": "First Row", "AxisPositions": null, "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Volts": "0.0000", "528-Name": "DA Shutter", "555-State": "0", "XLIGHT Dichroic-Label": "Di03-R635-t1", "445 AO-Sequenceable": "1", "405-Name": "DA Shutter", "405 AO-IOChannel": "Dev1/ao0", "Core-Camera": "PRIME", "Focus-Name": "Focus", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "405 Shutter-IOChannel": "Dev1/ao8", "640 AO-Demo": "No", "640 Shutter-SupportsTriggering": "0", "445 AO-Demo": "No", "555-DA Device": "555 Shutter", "470 Shutter-Description": "NI DAC", "528 AO-Volts": "0.0000", "COM1-DataBits": "8", "Vincent-D3-Address": "x", "520 AO-SupportsTriggering": "0", "405-Description": "DA device that is used as a shutter", "640 Shutter-MinVolts": "0.0000", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "405 AO-Name": "AnalogIO", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "640 AO-TriggerInputLine": "/Dev1/PFI0", "405 AO-TriggerInputLine": "/Dev1/PFI0", "640-Description": "DA device that is used as a shutter", "445 Shutter-TriggerSampleRate": "100000", "470 Shutter-TriggerSequenceLength": "1000", "COM1-StopBits": "1", "520 Shutter-SupportsTriggering": "0", "405-State": "0", "Core-Initialize": "1", "XLIGHT Emission Wheel-State": "3", "520 AO-Description": "NI DAC", "528 AO-TriggerInputLine": "/Dev1/PFI0", "445 Shutter-MaxVolts": "5.0000", "COM9-Handshaking": "Off", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "XLIGHT Spin motor-Label": "On (spinning)", "PRIME-PP 5 ENABLED": "No", "UUID": "36384302-9ef1-4499-af3c-0fc717858567", "PRIME-TriggerMode": "Internal Trigger", "COM1-Handshaking": "Off", "PRIME-Timing-PostTriggerDelayNs": "49344000.0000", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "Focus-Position": "16732.9000", "528 Shutter-Block voltage": "false", "Channel": "P-DIC", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "555 Shutter-Demo": "No", "405 Shutter-Demo": "No", "528 AO-Name": "AnalogIO", "520-State": "0", "Core-Galvo": "", "405 Shutter-TriggerSampleRate": "100000", "Focus-FocusStepSize_(nm)": "100", "PRIME-PixelType": "16bit", "COM4-DataBits": "8", "470 AO-IOChannel": "Dev1/ao2", "520 AO-MinVolts": "0.0000", "COM9-Parity": "None", "PRIME-CircularBufferEnabled": "ON", "PRIME-PP 6 Reset": "No", "640 AO-Description": "NI DAC", "Source": "PRIME", "445 AO-MinVolts": "0.0000", "PIEZO-Name": "Stage", "445 AO-Description": "NI DAC", "PRIME-CircularBufferFrameCount": "46", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "520 AO-Volts": "0.0000", "528 Shutter-TriggerSampleRate": "100000", "XPositionUm": 23269.100000000002, "OpticalPath-State": "4", "PRIME-PP 3 THRESHOLD": "80", "PRIME-PVCAM Version": "3.7.1", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Gain": "1-HDR", "640 Shutter-TriggerSequenceLength": "1000", "528 AO-SupportsTriggering": "0", "445 AO-TriggerInputLine": "/Dev1/PFI0", "OpticalPath-Name": "OpticalPath", "PRIME-PP 4 ENABLED": "Yes", "PRIME-PVCAM Adapter Version": "1.3.29", "COM3-Name": "COM3", "405 AO-MaxVolts": "5.0000", "520 Shutter-Block voltage": "false", "PRIME-Timing-ClearingTimeNs": "49344000.0000", "Core-Focus": "PIEZO", "528 AO-Block voltage": "false", "TE2000-Description": "Nikon TE2000 hub", "528 AO-MinVolts": "0.0000", "COM4-Parity": "None", "Summary": { "Slices": 11, "ChContrastMin": [ 0, 0, 0, 0, 0, 0 ], "PixelAspect": 1, "SlicesFirst": true, "Interval_ms": 0, "Time": "2019-12-17 03:59:33 -0500", "Prefix": "seq_7", "InitialPositionList": [ { "Label": "Pos0", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [149.91], "Focus": [16657.7], "XYStage": [ 71629.7, 19152.9 ] }, "GridColumnIndex": 0 }, { "Label": "Pos1", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [190.82], "Focus": [16485.4], "XYStage": [ 65702.3, 19396.600000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos2", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [194.69], "Focus": [16446.8], "XYStage": [ 59880.5, 19007.8 ] }, "GridColumnIndex": 0 }, { "Label": "Pos3", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [145.04], "Focus": [16446.6], "XYStage": [ 53616.9, 18818.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos4", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [169.57500000000002], "Focus": [16446.5], "XYStage": [ 47662.8, 18817.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos5", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [131.805], "Focus": [16446.4], "XYStage": [ 41918.8, 18298.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos6", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [113.145], "Focus": [16446.5], "XYStage": [ 36129.9, 18895.8 ] }, "GridColumnIndex": 0 }, { "Label": "Pos7", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [141.005], "Focus": [16446.6], "XYStage": [ 30299.100000000002, 18895.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos8", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [140.97], "Focus": [16446.5], "XYStage": [ 23952.100000000002, 19156 ] }, "GridColumnIndex": 0 }, { "Label": "Pos9", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [184.57500000000002], "Focus": [16677.9], "XYStage": [ 72866.8, -14809.300000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos10", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [167.95000000000002], "Focus": [16557.9], "XYStage": [ 66515.6, -14371.300000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos11", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [168], "Focus": [16618.9], "XYStage": [ 59724.3, -13964.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos12", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [203.205], "Focus": [16779.6], "XYStage": [ 53862.3, -13494.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos13", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [203.20000000000002], "Focus": [16749], "XYStage": [ 48108.3, -13805.400000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos14", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [203.19], "Focus": [16675.9], "XYStage": [ 41645, -13804.900000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos15", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [203.21], "Focus": [16675.7], "XYStage": [ 35352.6, -13805.6 ] }, "GridColumnIndex": 0 }, { "Label": "Pos16", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [203.225], "Focus": [16675.8], "XYStage": [ 29298, -13447.400000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos17", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [203.215], "Focus": [16675.8], "XYStage": [ 23269.100000000002, -13012.300000000001 ] }, "GridColumnIndex": 0 } ], "z-step_um": 10, "GridRow": 0, "BitDepth": 16, "Depth": 2, "Channels": 6, "Source": "Micro-Manager", "ChColors": [ -16711681, -256, -65281, -1, -1, -16776961 ], "MetadataVersion": 10, "KeepShutterOpenChannels": true, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536, 65536 ], "CustomIntervals_ms": [], "Height": 1200, "MicroManagerVersion": "1.4.23 20180219", "GridColumn": 0, "UUID": "3ad8801b-5699-40d6-813b-4ad840863e38", "PixelType": "GRAY16", "Width": 1200, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\Yan\\20191216-YC113-S11S12-miseq", "Frames": 1, "Comment": "", "UserName": "Nikon", "PixelSize_um": 1.1, "ROI": [ 0, 0, 1200, 1200 ], "Date": "2019-12-17", "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC", "P-DAPI-high" ], "KeepShutterOpenSlices": true, "IJType": 1, "ComputerName": "ZadorConfocal2", "Positions": 18, "PositionIndex": 17, "CameraTimeout": 20000, "TimeFirst": false }, "640 AO-Name": "AnalogIO", "Vincent-D3-Command": "Close" }, "FrameKey-0-4-5": { "COM4-StopBits": "1", "528 AO-MaxVolts": "5.0000", "XLIGHT Excitation Wheel-State": "1", "470 Shutter-Name": "AnalogIO", "Core-Shutter": "Vincent-D3", "555 AO-Demo": "No", "XLIGHT Dichroic-State": "2", "XYStage-StepSize-Y": "0.1000", "ZPositionUm": 203.215, "XYStage-StepSize-X": "0.1000", "PRIME-FirmwareVersion": "20.28", "PRIME-ClearCycles": "2", "520-Name": "DA Shutter", "405 Shutter-Name": "AnalogIO", "PIEZO-StepSize": "0.0050", "PRIME-ClearMode": "Pre-Exposure", "470 AO-MinVolts": "0.0000", "405 AO-SupportsTriggering": "1", "CameraChannelIndex": 0, "XYStage-StartSpeed": "500.0000", "COM9-StopBits": "2", "405 AO-Description": "NI DAC", "COM4-DelayBetweenCharsMs": "0.0000", "445 AO-Block voltage": "false", "520 AO-TriggerInputLine": "/Dev1/PFI0", "555 AO-Description": "NI DAC", "520 AO-TriggerSampleRate": "100000", "COM4-Verbose": "1", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM4-Name": "COM4", "405 Shutter-Volts": "0.0000", "Vincent-D3-Port": "COM1", "PRIME-PP 4 MIN ADU AFFECTED": "200", "470 Shutter-MinVolts": "0.0000", "PRIME-PP 3 ENABLED": "Yes", "470 AO-Description": "NI DAC", "PRIME-Description": "PVCAM API device adapter", "520 Shutter-MaxVolts": "5.0000", "520 Shutter-Volts": "0.0000", "528 AO-Sequenceable": "1", "PRIME-Binning": "1x1", "555 Shutter-MaxVolts": "5.0000", "528 Shutter-TriggerSequenceLength": "1000", "405 Shutter-Block voltage": "false", "XLIGHT Spin motor-State": "1", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "Vincent-D3-Time to open (ms)": "35.0000", "Vincent-D3-Time to close (ms)": "35.0000", "445 AO-TriggerSampleRate": "100000", "640 Shutter-IOChannel": "Dev1/ao14", "555 AO-Sequenceable": "1", "520 AO-Name": "AnalogIO", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "470 AO-Block voltage": "false", "COM1-Verbose": "1", "COM9-AnswerTimeout": "10000.0000", "528 Shutter-Name": "AnalogIO", "PRIME-Trigger Timeout (secs)": "10", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "NextFrame": 0, "470 AO-TriggerSequenceLength": "1000", "PRIME-TransposeXY": "0", "445 AO-TriggerSequenceLength": "1000", "COM1-Name": "COM1", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "COM9-DataBits": "8", "COM1-AnswerTimeout": "500.0000", "Camera": "", "TE2000-Version": "V2.50-V2.50-V2.10", "528 Shutter-Volts": "0.0000", "XLIGHT Hub-Name": "XLIGHT Hub", "PIEZO-Description": "Ludl stage driver adapter", "PositionName": "Pos17", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSampleRate": "100000", "PRIME-FanSpeedSetpoint": "High", "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "405 AO-TriggerSampleRate": "100000", "470 Shutter-Block voltage": "false", "470-State": "0", "COM3-Parity": "None", "405 AO-Volts": "0.0000", "PRIME-TransposeMirrorY": "0", "PRIME-ChipName": "GS144BSI", "470 AO-Volts": "0.0000", "PRIME-TransposeMirrorX": "0", "445 Shutter-SupportsTriggering": "0", "470 Shutter-SupportsTriggering": "0", "XLIGHT Hub-Description": "XLIGHT hub", "PRIME-PreampOffLimit": "10000", "470 AO-Sequenceable": "1", "528 Shutter-MaxVolts": "5.0000", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "Core-XYStage": "XYStage", "470 AO-TriggerSampleRate": "100000", "PRIME-Timing-PreTriggerDelayNs": "24672000.0000", "528-DA Device": "528 Shutter", "Focus-Description": "Nikon TE2000 focus adapter", "FilterBlock-State": "5", "520 AO-Demo": "No", "COM3-DelayBetweenCharsMs": "0.0000", "470 Shutter-Volts": "0.0000", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "PRIME-PP 2 THRESHOLD": "125", "PRIME-PP 4 THRESHOLD": "75", "XYStage-Acceleration": "4.0000", "470 Shutter-Demo": "No", "PRIME-Name": "PMPCIECam00", "640-DA Device": "640 Shutter", "405 AO-Block voltage": "false", "ElapsedTime-ms": 745386, "PRIME-Offset": "100", "445 AO-IOChannel": "Dev1/ao1", "640 AO-MaxVolts": "5.0000", "PositionIndex": 17, "PRIME-PP 1 ENABLED": "Yes", "COM1-BaudRate": "9600", "640-State": "0", "COM3-Verbose": "1", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "LudlController-TransmissionDelay": "0", "PRIME-FullWellCapacity": "62000", "PRIME-SMARTStreamingEnabled": "No", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "640 Shutter-Description": "NI DAC", "528-Description": "DA device that is used as a shutter", "COM4-Description": "Serial port driver (boost:asio)", "405 Shutter-Description": "NI DAC", "TE2000-Name": "TE2000", "528 Shutter-Description": "NI DAC", "COM9-Description": "Serial port driver (boost:asio)", "LudlController-Name": "LudlController", "445 Shutter-TriggerSequenceLength": "1000", "PRIME-SerialNumber": "A18B203008", "445 AO-Volts": "0.0000", "PRIME-X-dimension": "1200", "PRIME-ShutterCloseDelay": "0", "XLIGHT Disk slider-State": "2", "Position": "Default", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 AO-IOChannel": "Dev1/ao5", "520 AO-IOChannel": "Dev1/ao4", "528 AO-TriggerSequenceLength": "1000", "405 Shutter-MaxVolts": "5.0000", "470-DA Device": "470 Shutter", "PRIME-FTCapable": "No", "Core-ChannelGroup": "Channels", "555 AO-SupportsTriggering": "0", "PRIME-Y-dimension": "1200", "Frame": 0, "555 Shutter-Sequenceable": "1", "PRIME-Timing-ExposureTimeNs": "499998000.0000", "Core-ImageProcessor": "", "528-State": "0", "520 AO-Block voltage": "false", "XLIGHT Excitation Wheel-Label": "Excitation-2", "640 Shutter-Demo": "No", "COM3-StopBits": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "520 Shutter-IOChannel": "Dev1/ao11", "Width": 1200, "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-SupportsTriggering": "0", "640 AO-TriggerSampleRate": "100000", "520-Description": "DA device that is used as a shutter", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "405 Shutter-Sequenceable": "1", "405 Shutter-SupportsTriggering": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-TriggerSampleRate": "100000", "640 Shutter-MaxVolts": "5.0000", "555 AO-MaxVolts": "5.0000", "520 AO-TriggerSequenceLength": "1000", "COM3-BaudRate": "9600", "COM9-BaudRate": "9600", "Vincent-D3-Channel #": "Ch. #1", "PRIME-PMode": "Normal", "OpticalPath-Label": "Left(100)", "BitDepth": 16, "555 AO-TriggerSequenceLength": "1000", "445 Shutter-Description": "NI DAC", "405 AO-Sequenceable": "1", "PRIME-AcquisitionMethod": "Callbacks", "555 AO-Name": "AnalogIO", "PRIME-ShutterMode": "Pre-Exposure", "COM3-Handshaking": "Off", "445 AO-SupportsTriggering": "0", "ROI": "0-0-1200-1200", "COM9-Verbose": "1", "PRIME-Color": "OFF", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "PRIME-ShutterOpenDelay": "0", "COM1-Parity": "None", "Slice": 5, "SlicePosition": 0, "COM9-DelayBetweenCharsMs": "0.0000", "555-Name": "DA Shutter", "PIEZO-LudlSingleAxisName": "B", "XLIGHT Emission Wheel-Label": "Empty", "640 Shutter-Name": "AnalogIO", "640 AO-Sequenceable": "1", "520 Shutter-Demo": "No", "528 Shutter-Demo": "No", "470-Name": "DA Shutter", "520-DA Device": "520 Shutter", "XYStage-Description": "Ludl XY stage driver adapter", "445 Shutter-MinVolts": "0.0000", "528 AO-TriggerSampleRate": "100000", "405 AO-MinVolts": "0.0000", "405 Shutter-TriggerSequenceLength": "1000", "Height": 1200, "555 AO-Block voltage": "false", "640 Shutter-Volts": "0.0000", "PixelType": "GRAY16", "445 Shutter-Block voltage": "false", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "520 Shutter-TriggerSequenceLength": "1000", "COM9-Name": "COM9", "Core-SLM": "", "555 AO-Volts": "0.0000", "555 Shutter-Block voltage": "false", "XYStage-Speed": "1800.0000", "520 AO-MaxVolts": "5.0000", "528 Shutter-SupportsTriggering": "0", "COM1-Description": "Serial port driver (boost:asio)", "PRIME-MetadataEnabled": "No", "520 Shutter-MinVolts": "0.0000", "470 Shutter-Sequenceable": "1", "PRIME-PreampDelay": "0", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-MinVolts": "0.0000", "ChannelIndex": 4, "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Core-AutoShutter": "1", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "Exposure-ms": 20, "PRIME-PP 2 ENABLED": "Yes", "640 Shutter-TriggerSampleRate": "100000", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "470 Shutter-IOChannel": "Dev1/ao10", "405 AO-Demo": "No", "PixelSizeUm": 1.1, "470 AO-Demo": "No", "470 Shutter-MaxVolts": "5.0000", "Vincent-D3-Name": "Vincent-D3", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FileName": "img_000000000_P-DIC_005.tif", "LudlController-Reset": "Operate", "PRIME-Exposure": "20.00", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "445-Name": "DA Shutter", "555 Shutter-Name": "AnalogIO", "520 Shutter-TriggerSampleRate": "100000", "445 Shutter-IOChannel": "Dev1/ao9", "PRIME-TransposeCorrection": "0", "XYStage-StepSize": "0.1000", "445-Description": "DA device that is used as a shutter", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 AO-IOChannel": "Dev1/ao7", "520 Shutter-Name": "AnalogIO", "COM3-DataBits": "8", "640 AO-Volts": "0.0000", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "555 Shutter-IOChannel": "Dev1/ao13", "WaitInterval": null, "520 Shutter-Sequenceable": "1", "YPositionUm": -13012.300000000001, "445-State": "0", "445-DA Device": "445 Shutter", "PRIME-CircularBufferAutoSize": "ON", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "555 AO-TriggerSampleRate": "100000", "445 Shutter-Volts": "0.0000", "XLIGHT Hub-Port": "COM3", "Core-AutoFocus": "", "COM4-Handshaking": "Off", "528 Shutter-MinVolts": "0.0000", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "445 Shutter-Name": "AnalogIO", "528 Shutter-Sequenceable": "1", "XYStage-TransposeMirrorX": "0", "XYStage-TransposeMirrorY": "0", "PRIME-ReadoutRate": "100MHz 16bit", "XYStage-Name": "Stage", "640 AO-SupportsTriggering": "0", "640-Name": "DA Shutter", "640 Shutter-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "640 AO-Block voltage": "false", "445 Shutter-Sequenceable": "1", "470 AO-MaxVolts": "5.0000", "COM4-BaudRate": "9600", "470-Description": "DA device that is used as a shutter", "520 Shutter-Description": "NI DAC", "PRIME-CCDTemperature": "-14.9200", "Time": "2019-12-17 04:00:02 -0500", "640 AO-TriggerSequenceLength": "1000", "470 AO-Name": "AnalogIO", "XLIGHT Disk slider-Label": "Disk Pos 40um", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "Binning": "1x1", "TE2000-ModelType": "1", "COM3-AnswerTimeout": "500.0000", "555 Shutter-SupportsTriggering": "0", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "TE2000-Port": "COM4", "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Description": "NI DAC", "COM4-AnswerTimeout": "500.0000", "COM1-DelayBetweenCharsMs": "0.0000", "FilterBlock-Label": "Confocal", "445 AO-Name": "AnalogIO", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-Timing-ReadoutTimeNs": "24672000.0000", "528 AO-Demo": "No", "520 AO-Sequenceable": "1", "555-Description": "DA device that is used as a shutter", "405-DA Device": "405 Shutter", "405 AO-TriggerSequenceLength": "1000", "640 AO-MinVolts": "0.0000", "640 Shutter-Block voltage": "false", "FrameIndex": 0, "PRIME-Port": "CMOS", "555 AO-MinVolts": "0.0000", "COM3-Description": "Serial port driver (boost:asio)", "528 AO-Description": "NI DAC", "555 AO-IOChannel": "Dev1/ao6", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "555 AO-TriggerInputLine": "/Dev1/PFI0", "PRIME-ActualInterval-ms": "84.6200", "LudlController-Port": "COM9", "445 Shutter-Demo": "No", "PRIME-Trigger-Expose Out-Mux": "1", "SliceIndex": 5, "PRIME-CCDTemperatureSetPoint": "-15.0000", "405 Shutter-MinVolts": "0.0000", "PRIME-ExposeOutMode": "First Row", "AxisPositions": null, "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Volts": "0.0000", "528-Name": "DA Shutter", "555-State": "0", "XLIGHT Dichroic-Label": "Di03-R635-t1", "445 AO-Sequenceable": "1", "405-Name": "DA Shutter", "405 AO-IOChannel": "Dev1/ao0", "Core-Camera": "PRIME", "Focus-Name": "Focus", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "405 Shutter-IOChannel": "Dev1/ao8", "640 AO-Demo": "No", "640 Shutter-SupportsTriggering": "0", "445 AO-Demo": "No", "555-DA Device": "555 Shutter", "470 Shutter-Description": "NI DAC", "528 AO-Volts": "0.0000", "COM1-DataBits": "8", "Vincent-D3-Address": "x", "520 AO-SupportsTriggering": "0", "405-Description": "DA device that is used as a shutter", "640 Shutter-MinVolts": "0.0000", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "405 AO-Name": "AnalogIO", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "640 AO-TriggerInputLine": "/Dev1/PFI0", "405 AO-TriggerInputLine": "/Dev1/PFI0", "640-Description": "DA device that is used as a shutter", "445 Shutter-TriggerSampleRate": "100000", "470 Shutter-TriggerSequenceLength": "1000", "COM1-StopBits": "1", "520 Shutter-SupportsTriggering": "0", "405-State": "0", "Core-Initialize": "1", "XLIGHT Emission Wheel-State": "3", "520 AO-Description": "NI DAC", "528 AO-TriggerInputLine": "/Dev1/PFI0", "445 Shutter-MaxVolts": "5.0000", "COM9-Handshaking": "Off", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "XLIGHT Spin motor-Label": "On (spinning)", "PRIME-PP 5 ENABLED": "No", "UUID": "27336774-a792-41cd-81f3-ab74ded1466d", "PRIME-TriggerMode": "Internal Trigger", "COM1-Handshaking": "Off", "PRIME-Timing-PostTriggerDelayNs": "49344000.0000", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "Focus-Position": "16732.9000", "528 Shutter-Block voltage": "false", "Channel": "P-DIC", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "555 Shutter-Demo": "No", "405 Shutter-Demo": "No", "528 AO-Name": "AnalogIO", "520-State": "0", "Core-Galvo": "", "405 Shutter-TriggerSampleRate": "100000", "Focus-FocusStepSize_(nm)": "100", "PRIME-PixelType": "16bit", "COM4-DataBits": "8", "470 AO-IOChannel": "Dev1/ao2", "520 AO-MinVolts": "0.0000", "COM9-Parity": "None", "PRIME-CircularBufferEnabled": "ON", "PRIME-PP 6 Reset": "No", "640 AO-Description": "NI DAC", "Source": "PRIME", "445 AO-MinVolts": "0.0000", "PIEZO-Name": "Stage", "445 AO-Description": "NI DAC", "PRIME-CircularBufferFrameCount": "46", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "520 AO-Volts": "0.0000", "528 Shutter-TriggerSampleRate": "100000", "XPositionUm": 23269.100000000002, "OpticalPath-State": "4", "PRIME-PP 3 THRESHOLD": "80", "PRIME-PVCAM Version": "3.7.1", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Gain": "1-HDR", "640 Shutter-TriggerSequenceLength": "1000", "528 AO-SupportsTriggering": "0", "445 AO-TriggerInputLine": "/Dev1/PFI0", "OpticalPath-Name": "OpticalPath", "PRIME-PP 4 ENABLED": "Yes", "PRIME-PVCAM Adapter Version": "1.3.29", "COM3-Name": "COM3", "405 AO-MaxVolts": "5.0000", "520 Shutter-Block voltage": "false", "PRIME-Timing-ClearingTimeNs": "49344000.0000", "Core-Focus": "PIEZO", "528 AO-Block voltage": "false", "TE2000-Description": "Nikon TE2000 hub", "528 AO-MinVolts": "0.0000", "COM4-Parity": "None", "Summary": { "Slices": 11, "ChContrastMin": [ 0, 0, 0, 0, 0, 0 ], "PixelAspect": 1, "SlicesFirst": true, "Interval_ms": 0, "Time": "2019-12-17 03:59:33 -0500", "Prefix": "seq_7", "InitialPositionList": [ { "Label": "Pos0", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [149.91], "Focus": [16657.7], "XYStage": [ 71629.7, 19152.9 ] }, "GridColumnIndex": 0 }, { "Label": "Pos1", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [190.82], "Focus": [16485.4], "XYStage": [ 65702.3, 19396.600000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos2", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [194.69], "Focus": [16446.8], "XYStage": [ 59880.5, 19007.8 ] }, "GridColumnIndex": 0 }, { "Label": "Pos3", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [145.04], "Focus": [16446.6], "XYStage": [ 53616.9, 18818.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos4", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [169.57500000000002], "Focus": [16446.5], "XYStage": [ 47662.8, 18817.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos5", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [131.805], "Focus": [16446.4], "XYStage": [ 41918.8, 18298.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos6", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [113.145], "Focus": [16446.5], "XYStage": [ 36129.9, 18895.8 ] }, "GridColumnIndex": 0 }, { "Label": "Pos7", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [141.005], "Focus": [16446.6], "XYStage": [ 30299.100000000002, 18895.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos8", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [140.97], "Focus": [16446.5], "XYStage": [ 23952.100000000002, 19156 ] }, "GridColumnIndex": 0 }, { "Label": "Pos9", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [184.57500000000002], "Focus": [16677.9], "XYStage": [ 72866.8, -14809.300000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos10", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [167.95000000000002], "Focus": [16557.9], "XYStage": [ 66515.6, -14371.300000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos11", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [168], "Focus": [16618.9], "XYStage": [ 59724.3, -13964.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos12", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [203.205], "Focus": [16779.6], "XYStage": [ 53862.3, -13494.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos13", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [203.20000000000002], "Focus": [16749], "XYStage": [ 48108.3, -13805.400000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos14", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [203.19], "Focus": [16675.9], "XYStage": [ 41645, -13804.900000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos15", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [203.21], "Focus": [16675.7], "XYStage": [ 35352.6, -13805.6 ] }, "GridColumnIndex": 0 }, { "Label": "Pos16", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [203.225], "Focus": [16675.8], "XYStage": [ 29298, -13447.400000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos17", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [203.215], "Focus": [16675.8], "XYStage": [ 23269.100000000002, -13012.300000000001 ] }, "GridColumnIndex": 0 } ], "z-step_um": 10, "GridRow": 0, "BitDepth": 16, "Depth": 2, "Channels": 6, "Source": "Micro-Manager", "ChColors": [ -16711681, -256, -65281, -1, -1, -16776961 ], "MetadataVersion": 10, "KeepShutterOpenChannels": true, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536, 65536 ], "CustomIntervals_ms": [], "Height": 1200, "MicroManagerVersion": "1.4.23 20180219", "GridColumn": 0, "UUID": "3ad8801b-5699-40d6-813b-4ad840863e38", "PixelType": "GRAY16", "Width": 1200, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\Yan\\20191216-YC113-S11S12-miseq", "Frames": 1, "Comment": "", "UserName": "Nikon", "PixelSize_um": 1.1, "ROI": [ 0, 0, 1200, 1200 ], "Date": "2019-12-17", "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC", "P-DAPI-high" ], "KeepShutterOpenSlices": true, "IJType": 1, "ComputerName": "ZadorConfocal2", "Positions": 18, "PositionIndex": 17, "CameraTimeout": 20000, "TimeFirst": false }, "640 AO-Name": "AnalogIO", "Vincent-D3-Command": "Close" }, "FrameKey-0-4-6": { "COM4-StopBits": "1", "528 AO-MaxVolts": "5.0000", "XLIGHT Excitation Wheel-State": "1", "470 Shutter-Name": "AnalogIO", "Core-Shutter": "Vincent-D3", "555 AO-Demo": "No", "XLIGHT Dichroic-State": "2", "XYStage-StepSize-Y": "0.1000", "ZPositionUm": 213.215, "XYStage-StepSize-X": "0.1000", "PRIME-FirmwareVersion": "20.28", "PRIME-ClearCycles": "2", "520-Name": "DA Shutter", "405 Shutter-Name": "AnalogIO", "PIEZO-StepSize": "0.0050", "PRIME-ClearMode": "Pre-Exposure", "470 AO-MinVolts": "0.0000", "405 AO-SupportsTriggering": "1", "CameraChannelIndex": 0, "XYStage-StartSpeed": "500.0000", "COM9-StopBits": "2", "405 AO-Description": "NI DAC", "COM4-DelayBetweenCharsMs": "0.0000", "445 AO-Block voltage": "false", "520 AO-TriggerInputLine": "/Dev1/PFI0", "555 AO-Description": "NI DAC", "520 AO-TriggerSampleRate": "100000", "COM4-Verbose": "1", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM4-Name": "COM4", "405 Shutter-Volts": "0.0000", "Vincent-D3-Port": "COM1", "PRIME-PP 4 MIN ADU AFFECTED": "200", "470 Shutter-MinVolts": "0.0000", "PRIME-PP 3 ENABLED": "Yes", "470 AO-Description": "NI DAC", "PRIME-Description": "PVCAM API device adapter", "520 Shutter-MaxVolts": "5.0000", "520 Shutter-Volts": "0.0000", "528 AO-Sequenceable": "1", "PRIME-Binning": "1x1", "555 Shutter-MaxVolts": "5.0000", "528 Shutter-TriggerSequenceLength": "1000", "405 Shutter-Block voltage": "false", "XLIGHT Spin motor-State": "1", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "Vincent-D3-Time to open (ms)": "35.0000", "Vincent-D3-Time to close (ms)": "35.0000", "445 AO-TriggerSampleRate": "100000", "640 Shutter-IOChannel": "Dev1/ao14", "555 AO-Sequenceable": "1", "520 AO-Name": "AnalogIO", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "470 AO-Block voltage": "false", "COM1-Verbose": "1", "COM9-AnswerTimeout": "10000.0000", "528 Shutter-Name": "AnalogIO", "PRIME-Trigger Timeout (secs)": "10", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "NextFrame": 0, "470 AO-TriggerSequenceLength": "1000", "PRIME-TransposeXY": "0", "445 AO-TriggerSequenceLength": "1000", "COM1-Name": "COM1", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "COM9-DataBits": "8", "COM1-AnswerTimeout": "500.0000", "Camera": "", "TE2000-Version": "V2.50-V2.50-V2.10", "528 Shutter-Volts": "0.0000", "XLIGHT Hub-Name": "XLIGHT Hub", "PIEZO-Description": "Ludl stage driver adapter", "PositionName": "Pos17", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSampleRate": "100000", "PRIME-FanSpeedSetpoint": "High", "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "405 AO-TriggerSampleRate": "100000", "470 Shutter-Block voltage": "false", "470-State": "0", "COM3-Parity": "None", "405 AO-Volts": "0.0000", "PRIME-TransposeMirrorY": "0", "PRIME-ChipName": "GS144BSI", "470 AO-Volts": "0.0000", "PRIME-TransposeMirrorX": "0", "445 Shutter-SupportsTriggering": "0", "470 Shutter-SupportsTriggering": "0", "XLIGHT Hub-Description": "XLIGHT hub", "PRIME-PreampOffLimit": "10000", "470 AO-Sequenceable": "1", "528 Shutter-MaxVolts": "5.0000", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "Core-XYStage": "XYStage", "470 AO-TriggerSampleRate": "100000", "PRIME-Timing-PreTriggerDelayNs": "24672000.0000", "528-DA Device": "528 Shutter", "Focus-Description": "Nikon TE2000 focus adapter", "FilterBlock-State": "5", "520 AO-Demo": "No", "COM3-DelayBetweenCharsMs": "0.0000", "470 Shutter-Volts": "0.0000", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "PRIME-PP 2 THRESHOLD": "125", "PRIME-PP 4 THRESHOLD": "75", "XYStage-Acceleration": "4.0000", "470 Shutter-Demo": "No", "PRIME-Name": "PMPCIECam00", "640-DA Device": "640 Shutter", "405 AO-Block voltage": "false", "ElapsedTime-ms": 745658, "PRIME-Offset": "100", "445 AO-IOChannel": "Dev1/ao1", "640 AO-MaxVolts": "5.0000", "PositionIndex": 17, "PRIME-PP 1 ENABLED": "Yes", "COM1-BaudRate": "9600", "640-State": "0", "COM3-Verbose": "1", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "LudlController-TransmissionDelay": "0", "PRIME-FullWellCapacity": "62000", "PRIME-SMARTStreamingEnabled": "No", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "640 Shutter-Description": "NI DAC", "528-Description": "DA device that is used as a shutter", "COM4-Description": "Serial port driver (boost:asio)", "405 Shutter-Description": "NI DAC", "TE2000-Name": "TE2000", "528 Shutter-Description": "NI DAC", "COM9-Description": "Serial port driver (boost:asio)", "LudlController-Name": "LudlController", "445 Shutter-TriggerSequenceLength": "1000", "PRIME-SerialNumber": "A18B203008", "445 AO-Volts": "0.0000", "PRIME-X-dimension": "1200", "PRIME-ShutterCloseDelay": "0", "XLIGHT Disk slider-State": "2", "Position": "Default", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 AO-IOChannel": "Dev1/ao5", "520 AO-IOChannel": "Dev1/ao4", "528 AO-TriggerSequenceLength": "1000", "405 Shutter-MaxVolts": "5.0000", "470-DA Device": "470 Shutter", "PRIME-FTCapable": "No", "Core-ChannelGroup": "Channels", "555 AO-SupportsTriggering": "0", "PRIME-Y-dimension": "1200", "Frame": 0, "555 Shutter-Sequenceable": "1", "PRIME-Timing-ExposureTimeNs": "499998000.0000", "Core-ImageProcessor": "", "528-State": "0", "520 AO-Block voltage": "false", "XLIGHT Excitation Wheel-Label": "Excitation-2", "640 Shutter-Demo": "No", "COM3-StopBits": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "520 Shutter-IOChannel": "Dev1/ao11", "Width": 1200, "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-SupportsTriggering": "0", "640 AO-TriggerSampleRate": "100000", "520-Description": "DA device that is used as a shutter", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "405 Shutter-Sequenceable": "1", "405 Shutter-SupportsTriggering": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-TriggerSampleRate": "100000", "640 Shutter-MaxVolts": "5.0000", "555 AO-MaxVolts": "5.0000", "520 AO-TriggerSequenceLength": "1000", "COM3-BaudRate": "9600", "COM9-BaudRate": "9600", "Vincent-D3-Channel #": "Ch. #1", "PRIME-PMode": "Normal", "OpticalPath-Label": "Left(100)", "BitDepth": 16, "555 AO-TriggerSequenceLength": "1000", "445 Shutter-Description": "NI DAC", "405 AO-Sequenceable": "1", "PRIME-AcquisitionMethod": "Callbacks", "555 AO-Name": "AnalogIO", "PRIME-ShutterMode": "Pre-Exposure", "COM3-Handshaking": "Off", "445 AO-SupportsTriggering": "0", "ROI": "0-0-1200-1200", "COM9-Verbose": "1", "PRIME-Color": "OFF", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "PRIME-ShutterOpenDelay": "0", "COM1-Parity": "None", "Slice": 6, "SlicePosition": 10, "COM9-DelayBetweenCharsMs": "0.0000", "555-Name": "DA Shutter", "PIEZO-LudlSingleAxisName": "B", "XLIGHT Emission Wheel-Label": "Empty", "640 Shutter-Name": "AnalogIO", "640 AO-Sequenceable": "1", "520 Shutter-Demo": "No", "528 Shutter-Demo": "No", "470-Name": "DA Shutter", "520-DA Device": "520 Shutter", "XYStage-Description": "Ludl XY stage driver adapter", "445 Shutter-MinVolts": "0.0000", "528 AO-TriggerSampleRate": "100000", "405 AO-MinVolts": "0.0000", "405 Shutter-TriggerSequenceLength": "1000", "Height": 1200, "555 AO-Block voltage": "false", "640 Shutter-Volts": "0.0000", "PixelType": "GRAY16", "445 Shutter-Block voltage": "false", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "520 Shutter-TriggerSequenceLength": "1000", "COM9-Name": "COM9", "Core-SLM": "", "555 AO-Volts": "0.0000", "555 Shutter-Block voltage": "false", "XYStage-Speed": "1800.0000", "520 AO-MaxVolts": "5.0000", "528 Shutter-SupportsTriggering": "0", "COM1-Description": "Serial port driver (boost:asio)", "PRIME-MetadataEnabled": "No", "520 Shutter-MinVolts": "0.0000", "470 Shutter-Sequenceable": "1", "PRIME-PreampDelay": "0", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-MinVolts": "0.0000", "ChannelIndex": 4, "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Core-AutoShutter": "1", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "Exposure-ms": 20, "PRIME-PP 2 ENABLED": "Yes", "640 Shutter-TriggerSampleRate": "100000", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "470 Shutter-IOChannel": "Dev1/ao10", "405 AO-Demo": "No", "PixelSizeUm": 1.1, "470 AO-Demo": "No", "470 Shutter-MaxVolts": "5.0000", "Vincent-D3-Name": "Vincent-D3", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FileName": "img_000000000_P-DIC_006.tif", "LudlController-Reset": "Operate", "PRIME-Exposure": "20.00", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "445-Name": "DA Shutter", "555 Shutter-Name": "AnalogIO", "520 Shutter-TriggerSampleRate": "100000", "445 Shutter-IOChannel": "Dev1/ao9", "PRIME-TransposeCorrection": "0", "XYStage-StepSize": "0.1000", "445-Description": "DA device that is used as a shutter", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 AO-IOChannel": "Dev1/ao7", "520 Shutter-Name": "AnalogIO", "COM3-DataBits": "8", "640 AO-Volts": "0.0000", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "555 Shutter-IOChannel": "Dev1/ao13", "WaitInterval": null, "520 Shutter-Sequenceable": "1", "YPositionUm": -13012.300000000001, "445-State": "0", "445-DA Device": "445 Shutter", "PRIME-CircularBufferAutoSize": "ON", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "555 AO-TriggerSampleRate": "100000", "445 Shutter-Volts": "0.0000", "XLIGHT Hub-Port": "COM3", "Core-AutoFocus": "", "COM4-Handshaking": "Off", "528 Shutter-MinVolts": "0.0000", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "445 Shutter-Name": "AnalogIO", "528 Shutter-Sequenceable": "1", "XYStage-TransposeMirrorX": "0", "XYStage-TransposeMirrorY": "0", "PRIME-ReadoutRate": "100MHz 16bit", "XYStage-Name": "Stage", "640 AO-SupportsTriggering": "0", "640-Name": "DA Shutter", "640 Shutter-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "640 AO-Block voltage": "false", "445 Shutter-Sequenceable": "1", "470 AO-MaxVolts": "5.0000", "COM4-BaudRate": "9600", "470-Description": "DA device that is used as a shutter", "520 Shutter-Description": "NI DAC", "PRIME-CCDTemperature": "-14.9200", "Time": "2019-12-17 04:00:02 -0500", "640 AO-TriggerSequenceLength": "1000", "470 AO-Name": "AnalogIO", "XLIGHT Disk slider-Label": "Disk Pos 40um", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "Binning": "1x1", "TE2000-ModelType": "1", "COM3-AnswerTimeout": "500.0000", "555 Shutter-SupportsTriggering": "0", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "TE2000-Port": "COM4", "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Description": "NI DAC", "COM4-AnswerTimeout": "500.0000", "COM1-DelayBetweenCharsMs": "0.0000", "FilterBlock-Label": "Confocal", "445 AO-Name": "AnalogIO", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-Timing-ReadoutTimeNs": "24672000.0000", "528 AO-Demo": "No", "520 AO-Sequenceable": "1", "555-Description": "DA device that is used as a shutter", "405-DA Device": "405 Shutter", "405 AO-TriggerSequenceLength": "1000", "640 AO-MinVolts": "0.0000", "640 Shutter-Block voltage": "false", "FrameIndex": 0, "PRIME-Port": "CMOS", "555 AO-MinVolts": "0.0000", "COM3-Description": "Serial port driver (boost:asio)", "528 AO-Description": "NI DAC", "555 AO-IOChannel": "Dev1/ao6", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "555 AO-TriggerInputLine": "/Dev1/PFI0", "PRIME-ActualInterval-ms": "84.6200", "LudlController-Port": "COM9", "445 Shutter-Demo": "No", "PRIME-Trigger-Expose Out-Mux": "1", "SliceIndex": 6, "PRIME-CCDTemperatureSetPoint": "-15.0000", "405 Shutter-MinVolts": "0.0000", "PRIME-ExposeOutMode": "First Row", "AxisPositions": null, "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Volts": "0.0000", "528-Name": "DA Shutter", "555-State": "0", "XLIGHT Dichroic-Label": "Di03-R635-t1", "445 AO-Sequenceable": "1", "405-Name": "DA Shutter", "405 AO-IOChannel": "Dev1/ao0", "Core-Camera": "PRIME", "Focus-Name": "Focus", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "405 Shutter-IOChannel": "Dev1/ao8", "640 AO-Demo": "No", "640 Shutter-SupportsTriggering": "0", "445 AO-Demo": "No", "555-DA Device": "555 Shutter", "470 Shutter-Description": "NI DAC", "528 AO-Volts": "0.0000", "COM1-DataBits": "8", "Vincent-D3-Address": "x", "520 AO-SupportsTriggering": "0", "405-Description": "DA device that is used as a shutter", "640 Shutter-MinVolts": "0.0000", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "405 AO-Name": "AnalogIO", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "640 AO-TriggerInputLine": "/Dev1/PFI0", "405 AO-TriggerInputLine": "/Dev1/PFI0", "640-Description": "DA device that is used as a shutter", "445 Shutter-TriggerSampleRate": "100000", "470 Shutter-TriggerSequenceLength": "1000", "COM1-StopBits": "1", "520 Shutter-SupportsTriggering": "0", "405-State": "0", "Core-Initialize": "1", "XLIGHT Emission Wheel-State": "3", "520 AO-Description": "NI DAC", "528 AO-TriggerInputLine": "/Dev1/PFI0", "445 Shutter-MaxVolts": "5.0000", "COM9-Handshaking": "Off", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "XLIGHT Spin motor-Label": "On (spinning)", "PRIME-PP 5 ENABLED": "No", "UUID": "d7f6b35d-e9ab-4ab0-b5ec-74501ea5c458", "PRIME-TriggerMode": "Internal Trigger", "COM1-Handshaking": "Off", "PRIME-Timing-PostTriggerDelayNs": "49344000.0000", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "Focus-Position": "16732.9000", "528 Shutter-Block voltage": "false", "Channel": "P-DIC", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "555 Shutter-Demo": "No", "405 Shutter-Demo": "No", "528 AO-Name": "AnalogIO", "520-State": "0", "Core-Galvo": "", "405 Shutter-TriggerSampleRate": "100000", "Focus-FocusStepSize_(nm)": "100", "PRIME-PixelType": "16bit", "COM4-DataBits": "8", "470 AO-IOChannel": "Dev1/ao2", "520 AO-MinVolts": "0.0000", "COM9-Parity": "None", "PRIME-CircularBufferEnabled": "ON", "PRIME-PP 6 Reset": "No", "640 AO-Description": "NI DAC", "Source": "PRIME", "445 AO-MinVolts": "0.0000", "PIEZO-Name": "Stage", "445 AO-Description": "NI DAC", "PRIME-CircularBufferFrameCount": "46", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "520 AO-Volts": "0.0000", "528 Shutter-TriggerSampleRate": "100000", "XPositionUm": 23269.100000000002, "OpticalPath-State": "4", "PRIME-PP 3 THRESHOLD": "80", "PRIME-PVCAM Version": "3.7.1", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Gain": "1-HDR", "640 Shutter-TriggerSequenceLength": "1000", "528 AO-SupportsTriggering": "0", "445 AO-TriggerInputLine": "/Dev1/PFI0", "OpticalPath-Name": "OpticalPath", "PRIME-PP 4 ENABLED": "Yes", "PRIME-PVCAM Adapter Version": "1.3.29", "COM3-Name": "COM3", "405 AO-MaxVolts": "5.0000", "520 Shutter-Block voltage": "false", "PRIME-Timing-ClearingTimeNs": "49344000.0000", "Core-Focus": "PIEZO", "528 AO-Block voltage": "false", "TE2000-Description": "Nikon TE2000 hub", "528 AO-MinVolts": "0.0000", "COM4-Parity": "None", "Summary": { "Slices": 11, "ChContrastMin": [ 0, 0, 0, 0, 0, 0 ], "PixelAspect": 1, "SlicesFirst": true, "Interval_ms": 0, "Time": "2019-12-17 03:59:33 -0500", "Prefix": "seq_7", "InitialPositionList": [ { "Label": "Pos0", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [149.91], "Focus": [16657.7], "XYStage": [ 71629.7, 19152.9 ] }, "GridColumnIndex": 0 }, { "Label": "Pos1", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [190.82], "Focus": [16485.4], "XYStage": [ 65702.3, 19396.600000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos2", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [194.69], "Focus": [16446.8], "XYStage": [ 59880.5, 19007.8 ] }, "GridColumnIndex": 0 }, { "Label": "Pos3", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [145.04], "Focus": [16446.6], "XYStage": [ 53616.9, 18818.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos4", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [169.57500000000002], "Focus": [16446.5], "XYStage": [ 47662.8, 18817.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos5", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [131.805], "Focus": [16446.4], "XYStage": [ 41918.8, 18298.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos6", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [113.145], "Focus": [16446.5], "XYStage": [ 36129.9, 18895.8 ] }, "GridColumnIndex": 0 }, { "Label": "Pos7", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [141.005], "Focus": [16446.6], "XYStage": [ 30299.100000000002, 18895.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos8", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [140.97], "Focus": [16446.5], "XYStage": [ 23952.100000000002, 19156 ] }, "GridColumnIndex": 0 }, { "Label": "Pos9", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [184.57500000000002], "Focus": [16677.9], "XYStage": [ 72866.8, -14809.300000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos10", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [167.95000000000002], "Focus": [16557.9], "XYStage": [ 66515.6, -14371.300000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos11", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [168], "Focus": [16618.9], "XYStage": [ 59724.3, -13964.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos12", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [203.205], "Focus": [16779.6], "XYStage": [ 53862.3, -13494.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos13", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [203.20000000000002], "Focus": [16749], "XYStage": [ 48108.3, -13805.400000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos14", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [203.19], "Focus": [16675.9], "XYStage": [ 41645, -13804.900000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos15", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [203.21], "Focus": [16675.7], "XYStage": [ 35352.6, -13805.6 ] }, "GridColumnIndex": 0 }, { "Label": "Pos16", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [203.225], "Focus": [16675.8], "XYStage": [ 29298, -13447.400000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos17", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [203.215], "Focus": [16675.8], "XYStage": [ 23269.100000000002, -13012.300000000001 ] }, "GridColumnIndex": 0 } ], "z-step_um": 10, "GridRow": 0, "BitDepth": 16, "Depth": 2, "Channels": 6, "Source": "Micro-Manager", "ChColors": [ -16711681, -256, -65281, -1, -1, -16776961 ], "MetadataVersion": 10, "KeepShutterOpenChannels": true, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536, 65536 ], "CustomIntervals_ms": [], "Height": 1200, "MicroManagerVersion": "1.4.23 20180219", "GridColumn": 0, "UUID": "3ad8801b-5699-40d6-813b-4ad840863e38", "PixelType": "GRAY16", "Width": 1200, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\Yan\\20191216-YC113-S11S12-miseq", "Frames": 1, "Comment": "", "UserName": "Nikon", "PixelSize_um": 1.1, "ROI": [ 0, 0, 1200, 1200 ], "Date": "2019-12-17", "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC", "P-DAPI-high" ], "KeepShutterOpenSlices": true, "IJType": 1, "ComputerName": "ZadorConfocal2", "Positions": 18, "PositionIndex": 17, "CameraTimeout": 20000, "TimeFirst": false }, "640 AO-Name": "AnalogIO", "Vincent-D3-Command": "Close" }, "FrameKey-0-4-7": { "COM4-StopBits": "1", "528 AO-MaxVolts": "5.0000", "XLIGHT Excitation Wheel-State": "1", "470 Shutter-Name": "AnalogIO", "Core-Shutter": "Vincent-D3", "555 AO-Demo": "No", "XLIGHT Dichroic-State": "2", "XYStage-StepSize-Y": "0.1000", "ZPositionUm": 223.215, "XYStage-StepSize-X": "0.1000", "PRIME-FirmwareVersion": "20.28", "PRIME-ClearCycles": "2", "520-Name": "DA Shutter", "405 Shutter-Name": "AnalogIO", "PIEZO-StepSize": "0.0050", "PRIME-ClearMode": "Pre-Exposure", "470 AO-MinVolts": "0.0000", "405 AO-SupportsTriggering": "1", "CameraChannelIndex": 0, "XYStage-StartSpeed": "500.0000", "COM9-StopBits": "2", "405 AO-Description": "NI DAC", "COM4-DelayBetweenCharsMs": "0.0000", "445 AO-Block voltage": "false", "520 AO-TriggerInputLine": "/Dev1/PFI0", "555 AO-Description": "NI DAC", "520 AO-TriggerSampleRate": "100000", "COM4-Verbose": "1", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM4-Name": "COM4", "405 Shutter-Volts": "0.0000", "Vincent-D3-Port": "COM1", "PRIME-PP 4 MIN ADU AFFECTED": "200", "470 Shutter-MinVolts": "0.0000", "PRIME-PP 3 ENABLED": "Yes", "470 AO-Description": "NI DAC", "PRIME-Description": "PVCAM API device adapter", "520 Shutter-MaxVolts": "5.0000", "520 Shutter-Volts": "0.0000", "528 AO-Sequenceable": "1", "PRIME-Binning": "1x1", "555 Shutter-MaxVolts": "5.0000", "528 Shutter-TriggerSequenceLength": "1000", "405 Shutter-Block voltage": "false", "XLIGHT Spin motor-State": "1", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "Vincent-D3-Time to open (ms)": "35.0000", "Vincent-D3-Time to close (ms)": "35.0000", "445 AO-TriggerSampleRate": "100000", "640 Shutter-IOChannel": "Dev1/ao14", "555 AO-Sequenceable": "1", "520 AO-Name": "AnalogIO", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "470 AO-Block voltage": "false", "COM1-Verbose": "1", "COM9-AnswerTimeout": "10000.0000", "528 Shutter-Name": "AnalogIO", "PRIME-Trigger Timeout (secs)": "10", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "NextFrame": 0, "470 AO-TriggerSequenceLength": "1000", "PRIME-TransposeXY": "0", "445 AO-TriggerSequenceLength": "1000", "COM1-Name": "COM1", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "COM9-DataBits": "8", "COM1-AnswerTimeout": "500.0000", "Camera": "", "TE2000-Version": "V2.50-V2.50-V2.10", "528 Shutter-Volts": "0.0000", "XLIGHT Hub-Name": "XLIGHT Hub", "PIEZO-Description": "Ludl stage driver adapter", "PositionName": "Pos17", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSampleRate": "100000", "PRIME-FanSpeedSetpoint": "High", "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "405 AO-TriggerSampleRate": "100000", "470 Shutter-Block voltage": "false", "470-State": "0", "COM3-Parity": "None", "405 AO-Volts": "0.0000", "PRIME-TransposeMirrorY": "0", "PRIME-ChipName": "GS144BSI", "470 AO-Volts": "0.0000", "PRIME-TransposeMirrorX": "0", "445 Shutter-SupportsTriggering": "0", "470 Shutter-SupportsTriggering": "0", "XLIGHT Hub-Description": "XLIGHT hub", "PRIME-PreampOffLimit": "10000", "470 AO-Sequenceable": "1", "528 Shutter-MaxVolts": "5.0000", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "Core-XYStage": "XYStage", "470 AO-TriggerSampleRate": "100000", "PRIME-Timing-PreTriggerDelayNs": "24672000.0000", "528-DA Device": "528 Shutter", "Focus-Description": "Nikon TE2000 focus adapter", "FilterBlock-State": "5", "520 AO-Demo": "No", "COM3-DelayBetweenCharsMs": "0.0000", "470 Shutter-Volts": "0.0000", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "PRIME-PP 2 THRESHOLD": "125", "PRIME-PP 4 THRESHOLD": "75", "XYStage-Acceleration": "4.0000", "470 Shutter-Demo": "No", "PRIME-Name": "PMPCIECam00", "640-DA Device": "640 Shutter", "405 AO-Block voltage": "false", "ElapsedTime-ms": 745913, "PRIME-Offset": "100", "445 AO-IOChannel": "Dev1/ao1", "640 AO-MaxVolts": "5.0000", "PositionIndex": 17, "PRIME-PP 1 ENABLED": "Yes", "COM1-BaudRate": "9600", "640-State": "0", "COM3-Verbose": "1", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "LudlController-TransmissionDelay": "0", "PRIME-FullWellCapacity": "62000", "PRIME-SMARTStreamingEnabled": "No", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "640 Shutter-Description": "NI DAC", "528-Description": "DA device that is used as a shutter", "COM4-Description": "Serial port driver (boost:asio)", "405 Shutter-Description": "NI DAC", "TE2000-Name": "TE2000", "528 Shutter-Description": "NI DAC", "COM9-Description": "Serial port driver (boost:asio)", "LudlController-Name": "LudlController", "445 Shutter-TriggerSequenceLength": "1000", "PRIME-SerialNumber": "A18B203008", "445 AO-Volts": "0.0000", "PRIME-X-dimension": "1200", "PRIME-ShutterCloseDelay": "0", "XLIGHT Disk slider-State": "2", "Position": "Default", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 AO-IOChannel": "Dev1/ao5", "520 AO-IOChannel": "Dev1/ao4", "528 AO-TriggerSequenceLength": "1000", "405 Shutter-MaxVolts": "5.0000", "470-DA Device": "470 Shutter", "PRIME-FTCapable": "No", "Core-ChannelGroup": "Channels", "555 AO-SupportsTriggering": "0", "PRIME-Y-dimension": "1200", "Frame": 0, "555 Shutter-Sequenceable": "1", "PRIME-Timing-ExposureTimeNs": "499998000.0000", "Core-ImageProcessor": "", "528-State": "0", "520 AO-Block voltage": "false", "XLIGHT Excitation Wheel-Label": "Excitation-2", "640 Shutter-Demo": "No", "COM3-StopBits": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "520 Shutter-IOChannel": "Dev1/ao11", "Width": 1200, "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-SupportsTriggering": "0", "640 AO-TriggerSampleRate": "100000", "520-Description": "DA device that is used as a shutter", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "405 Shutter-Sequenceable": "1", "405 Shutter-SupportsTriggering": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-TriggerSampleRate": "100000", "640 Shutter-MaxVolts": "5.0000", "555 AO-MaxVolts": "5.0000", "520 AO-TriggerSequenceLength": "1000", "COM3-BaudRate": "9600", "COM9-BaudRate": "9600", "Vincent-D3-Channel #": "Ch. #1", "PRIME-PMode": "Normal", "OpticalPath-Label": "Left(100)", "BitDepth": 16, "555 AO-TriggerSequenceLength": "1000", "445 Shutter-Description": "NI DAC", "405 AO-Sequenceable": "1", "PRIME-AcquisitionMethod": "Callbacks", "555 AO-Name": "AnalogIO", "PRIME-ShutterMode": "Pre-Exposure", "COM3-Handshaking": "Off", "445 AO-SupportsTriggering": "0", "ROI": "0-0-1200-1200", "COM9-Verbose": "1", "PRIME-Color": "OFF", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "PRIME-ShutterOpenDelay": "0", "COM1-Parity": "None", "Slice": 7, "SlicePosition": 20, "COM9-DelayBetweenCharsMs": "0.0000", "555-Name": "DA Shutter", "PIEZO-LudlSingleAxisName": "B", "XLIGHT Emission Wheel-Label": "Empty", "640 Shutter-Name": "AnalogIO", "640 AO-Sequenceable": "1", "520 Shutter-Demo": "No", "528 Shutter-Demo": "No", "470-Name": "DA Shutter", "520-DA Device": "520 Shutter", "XYStage-Description": "Ludl XY stage driver adapter", "445 Shutter-MinVolts": "0.0000", "528 AO-TriggerSampleRate": "100000", "405 AO-MinVolts": "0.0000", "405 Shutter-TriggerSequenceLength": "1000", "Height": 1200, "555 AO-Block voltage": "false", "640 Shutter-Volts": "0.0000", "PixelType": "GRAY16", "445 Shutter-Block voltage": "false", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "520 Shutter-TriggerSequenceLength": "1000", "COM9-Name": "COM9", "Core-SLM": "", "555 AO-Volts": "0.0000", "555 Shutter-Block voltage": "false", "XYStage-Speed": "1800.0000", "520 AO-MaxVolts": "5.0000", "528 Shutter-SupportsTriggering": "0", "COM1-Description": "Serial port driver (boost:asio)", "PRIME-MetadataEnabled": "No", "520 Shutter-MinVolts": "0.0000", "470 Shutter-Sequenceable": "1", "PRIME-PreampDelay": "0", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-MinVolts": "0.0000", "ChannelIndex": 4, "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Core-AutoShutter": "1", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "Exposure-ms": 20, "PRIME-PP 2 ENABLED": "Yes", "640 Shutter-TriggerSampleRate": "100000", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "470 Shutter-IOChannel": "Dev1/ao10", "405 AO-Demo": "No", "PixelSizeUm": 1.1, "470 AO-Demo": "No", "470 Shutter-MaxVolts": "5.0000", "Vincent-D3-Name": "Vincent-D3", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FileName": "img_000000000_P-DIC_007.tif", "LudlController-Reset": "Operate", "PRIME-Exposure": "20.00", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "445-Name": "DA Shutter", "555 Shutter-Name": "AnalogIO", "520 Shutter-TriggerSampleRate": "100000", "445 Shutter-IOChannel": "Dev1/ao9", "PRIME-TransposeCorrection": "0", "XYStage-StepSize": "0.1000", "445-Description": "DA device that is used as a shutter", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 AO-IOChannel": "Dev1/ao7", "520 Shutter-Name": "AnalogIO", "COM3-DataBits": "8", "640 AO-Volts": "0.0000", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "555 Shutter-IOChannel": "Dev1/ao13", "WaitInterval": null, "520 Shutter-Sequenceable": "1", "YPositionUm": -13012.300000000001, "445-State": "0", "445-DA Device": "445 Shutter", "PRIME-CircularBufferAutoSize": "ON", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "555 AO-TriggerSampleRate": "100000", "445 Shutter-Volts": "0.0000", "XLIGHT Hub-Port": "COM3", "Core-AutoFocus": "", "COM4-Handshaking": "Off", "528 Shutter-MinVolts": "0.0000", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "445 Shutter-Name": "AnalogIO", "528 Shutter-Sequenceable": "1", "XYStage-TransposeMirrorX": "0", "XYStage-TransposeMirrorY": "0", "PRIME-ReadoutRate": "100MHz 16bit", "XYStage-Name": "Stage", "640 AO-SupportsTriggering": "0", "640-Name": "DA Shutter", "640 Shutter-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "640 AO-Block voltage": "false", "445 Shutter-Sequenceable": "1", "470 AO-MaxVolts": "5.0000", "COM4-BaudRate": "9600", "470-Description": "DA device that is used as a shutter", "520 Shutter-Description": "NI DAC", "PRIME-CCDTemperature": "-14.9200", "Time": "2019-12-17 04:00:02 -0500", "640 AO-TriggerSequenceLength": "1000", "470 AO-Name": "AnalogIO", "XLIGHT Disk slider-Label": "Disk Pos 40um", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "Binning": "1x1", "TE2000-ModelType": "1", "COM3-AnswerTimeout": "500.0000", "555 Shutter-SupportsTriggering": "0", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "TE2000-Port": "COM4", "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Description": "NI DAC", "COM4-AnswerTimeout": "500.0000", "COM1-DelayBetweenCharsMs": "0.0000", "FilterBlock-Label": "Confocal", "445 AO-Name": "AnalogIO", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-Timing-ReadoutTimeNs": "24672000.0000", "528 AO-Demo": "No", "520 AO-Sequenceable": "1", "555-Description": "DA device that is used as a shutter", "405-DA Device": "405 Shutter", "405 AO-TriggerSequenceLength": "1000", "640 AO-MinVolts": "0.0000", "640 Shutter-Block voltage": "false", "FrameIndex": 0, "PRIME-Port": "CMOS", "555 AO-MinVolts": "0.0000", "COM3-Description": "Serial port driver (boost:asio)", "528 AO-Description": "NI DAC", "555 AO-IOChannel": "Dev1/ao6", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "555 AO-TriggerInputLine": "/Dev1/PFI0", "PRIME-ActualInterval-ms": "84.6200", "LudlController-Port": "COM9", "445 Shutter-Demo": "No", "PRIME-Trigger-Expose Out-Mux": "1", "SliceIndex": 7, "PRIME-CCDTemperatureSetPoint": "-15.0000", "405 Shutter-MinVolts": "0.0000", "PRIME-ExposeOutMode": "First Row", "AxisPositions": null, "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Volts": "0.0000", "528-Name": "DA Shutter", "555-State": "0", "XLIGHT Dichroic-Label": "Di03-R635-t1", "445 AO-Sequenceable": "1", "405-Name": "DA Shutter", "405 AO-IOChannel": "Dev1/ao0", "Core-Camera": "PRIME", "Focus-Name": "Focus", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "405 Shutter-IOChannel": "Dev1/ao8", "640 AO-Demo": "No", "640 Shutter-SupportsTriggering": "0", "445 AO-Demo": "No", "555-DA Device": "555 Shutter", "470 Shutter-Description": "NI DAC", "528 AO-Volts": "0.0000", "COM1-DataBits": "8", "Vincent-D3-Address": "x", "520 AO-SupportsTriggering": "0", "405-Description": "DA device that is used as a shutter", "640 Shutter-MinVolts": "0.0000", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "405 AO-Name": "AnalogIO", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "640 AO-TriggerInputLine": "/Dev1/PFI0", "405 AO-TriggerInputLine": "/Dev1/PFI0", "640-Description": "DA device that is used as a shutter", "445 Shutter-TriggerSampleRate": "100000", "470 Shutter-TriggerSequenceLength": "1000", "COM1-StopBits": "1", "520 Shutter-SupportsTriggering": "0", "405-State": "0", "Core-Initialize": "1", "XLIGHT Emission Wheel-State": "3", "520 AO-Description": "NI DAC", "528 AO-TriggerInputLine": "/Dev1/PFI0", "445 Shutter-MaxVolts": "5.0000", "COM9-Handshaking": "Off", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "XLIGHT Spin motor-Label": "On (spinning)", "PRIME-PP 5 ENABLED": "No", "UUID": "8e0eade9-6f7f-4a28-a249-ead2d40b438f", "PRIME-TriggerMode": "Internal Trigger", "COM1-Handshaking": "Off", "PRIME-Timing-PostTriggerDelayNs": "49344000.0000", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "Focus-Position": "16732.9000", "528 Shutter-Block voltage": "false", "Channel": "P-DIC", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "555 Shutter-Demo": "No", "405 Shutter-Demo": "No", "528 AO-Name": "AnalogIO", "520-State": "0", "Core-Galvo": "", "405 Shutter-TriggerSampleRate": "100000", "Focus-FocusStepSize_(nm)": "100", "PRIME-PixelType": "16bit", "COM4-DataBits": "8", "470 AO-IOChannel": "Dev1/ao2", "520 AO-MinVolts": "0.0000", "COM9-Parity": "None", "PRIME-CircularBufferEnabled": "ON", "PRIME-PP 6 Reset": "No", "640 AO-Description": "NI DAC", "Source": "PRIME", "445 AO-MinVolts": "0.0000", "PIEZO-Name": "Stage", "445 AO-Description": "NI DAC", "PRIME-CircularBufferFrameCount": "46", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "520 AO-Volts": "0.0000", "528 Shutter-TriggerSampleRate": "100000", "XPositionUm": 23269.100000000002, "OpticalPath-State": "4", "PRIME-PP 3 THRESHOLD": "80", "PRIME-PVCAM Version": "3.7.1", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Gain": "1-HDR", "640 Shutter-TriggerSequenceLength": "1000", "528 AO-SupportsTriggering": "0", "445 AO-TriggerInputLine": "/Dev1/PFI0", "OpticalPath-Name": "OpticalPath", "PRIME-PP 4 ENABLED": "Yes", "PRIME-PVCAM Adapter Version": "1.3.29", "COM3-Name": "COM3", "405 AO-MaxVolts": "5.0000", "520 Shutter-Block voltage": "false", "PRIME-Timing-ClearingTimeNs": "49344000.0000", "Core-Focus": "PIEZO", "528 AO-Block voltage": "false", "TE2000-Description": "Nikon TE2000 hub", "528 AO-MinVolts": "0.0000", "COM4-Parity": "None", "Summary": { "Slices": 11, "ChContrastMin": [ 0, 0, 0, 0, 0, 0 ], "PixelAspect": 1, "SlicesFirst": true, "Interval_ms": 0, "Time": "2019-12-17 03:59:33 -0500", "Prefix": "seq_7", "InitialPositionList": [ { "Label": "Pos0", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [149.91], "Focus": [16657.7], "XYStage": [ 71629.7, 19152.9 ] }, "GridColumnIndex": 0 }, { "Label": "Pos1", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [190.82], "Focus": [16485.4], "XYStage": [ 65702.3, 19396.600000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos2", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [194.69], "Focus": [16446.8], "XYStage": [ 59880.5, 19007.8 ] }, "GridColumnIndex": 0 }, { "Label": "Pos3", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [145.04], "Focus": [16446.6], "XYStage": [ 53616.9, 18818.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos4", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [169.57500000000002], "Focus": [16446.5], "XYStage": [ 47662.8, 18817.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos5", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [131.805], "Focus": [16446.4], "XYStage": [ 41918.8, 18298.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos6", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [113.145], "Focus": [16446.5], "XYStage": [ 36129.9, 18895.8 ] }, "GridColumnIndex": 0 }, { "Label": "Pos7", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [141.005], "Focus": [16446.6], "XYStage": [ 30299.100000000002, 18895.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos8", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [140.97], "Focus": [16446.5], "XYStage": [ 23952.100000000002, 19156 ] }, "GridColumnIndex": 0 }, { "Label": "Pos9", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [184.57500000000002], "Focus": [16677.9], "XYStage": [ 72866.8, -14809.300000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos10", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [167.95000000000002], "Focus": [16557.9], "XYStage": [ 66515.6, -14371.300000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos11", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [168], "Focus": [16618.9], "XYStage": [ 59724.3, -13964.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos12", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [203.205], "Focus": [16779.6], "XYStage": [ 53862.3, -13494.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos13", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [203.20000000000002], "Focus": [16749], "XYStage": [ 48108.3, -13805.400000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos14", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [203.19], "Focus": [16675.9], "XYStage": [ 41645, -13804.900000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos15", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [203.21], "Focus": [16675.7], "XYStage": [ 35352.6, -13805.6 ] }, "GridColumnIndex": 0 }, { "Label": "Pos16", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [203.225], "Focus": [16675.8], "XYStage": [ 29298, -13447.400000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos17", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [203.215], "Focus": [16675.8], "XYStage": [ 23269.100000000002, -13012.300000000001 ] }, "GridColumnIndex": 0 } ], "z-step_um": 10, "GridRow": 0, "BitDepth": 16, "Depth": 2, "Channels": 6, "Source": "Micro-Manager", "ChColors": [ -16711681, -256, -65281, -1, -1, -16776961 ], "MetadataVersion": 10, "KeepShutterOpenChannels": true, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536, 65536 ], "CustomIntervals_ms": [], "Height": 1200, "MicroManagerVersion": "1.4.23 20180219", "GridColumn": 0, "UUID": "3ad8801b-5699-40d6-813b-4ad840863e38", "PixelType": "GRAY16", "Width": 1200, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\Yan\\20191216-YC113-S11S12-miseq", "Frames": 1, "Comment": "", "UserName": "Nikon", "PixelSize_um": 1.1, "ROI": [ 0, 0, 1200, 1200 ], "Date": "2019-12-17", "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC", "P-DAPI-high" ], "KeepShutterOpenSlices": true, "IJType": 1, "ComputerName": "ZadorConfocal2", "Positions": 18, "PositionIndex": 17, "CameraTimeout": 20000, "TimeFirst": false }, "640 AO-Name": "AnalogIO", "Vincent-D3-Command": "Close" }, "FrameKey-0-4-8": { "COM4-StopBits": "1", "528 AO-MaxVolts": "5.0000", "XLIGHT Excitation Wheel-State": "1", "470 Shutter-Name": "AnalogIO", "Core-Shutter": "Vincent-D3", "555 AO-Demo": "No", "XLIGHT Dichroic-State": "2", "XYStage-StepSize-Y": "0.1000", "ZPositionUm": 233.215, "XYStage-StepSize-X": "0.1000", "PRIME-FirmwareVersion": "20.28", "PRIME-ClearCycles": "2", "520-Name": "DA Shutter", "405 Shutter-Name": "AnalogIO", "PIEZO-StepSize": "0.0050", "PRIME-ClearMode": "Pre-Exposure", "470 AO-MinVolts": "0.0000", "405 AO-SupportsTriggering": "1", "CameraChannelIndex": 0, "XYStage-StartSpeed": "500.0000", "COM9-StopBits": "2", "405 AO-Description": "NI DAC", "COM4-DelayBetweenCharsMs": "0.0000", "445 AO-Block voltage": "false", "520 AO-TriggerInputLine": "/Dev1/PFI0", "555 AO-Description": "NI DAC", "520 AO-TriggerSampleRate": "100000", "COM4-Verbose": "1", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM4-Name": "COM4", "405 Shutter-Volts": "0.0000", "Vincent-D3-Port": "COM1", "PRIME-PP 4 MIN ADU AFFECTED": "200", "470 Shutter-MinVolts": "0.0000", "PRIME-PP 3 ENABLED": "Yes", "470 AO-Description": "NI DAC", "PRIME-Description": "PVCAM API device adapter", "520 Shutter-MaxVolts": "5.0000", "520 Shutter-Volts": "0.0000", "528 AO-Sequenceable": "1", "PRIME-Binning": "1x1", "555 Shutter-MaxVolts": "5.0000", "528 Shutter-TriggerSequenceLength": "1000", "405 Shutter-Block voltage": "false", "XLIGHT Spin motor-State": "1", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "Vincent-D3-Time to open (ms)": "35.0000", "Vincent-D3-Time to close (ms)": "35.0000", "445 AO-TriggerSampleRate": "100000", "640 Shutter-IOChannel": "Dev1/ao14", "555 AO-Sequenceable": "1", "520 AO-Name": "AnalogIO", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "470 AO-Block voltage": "false", "COM1-Verbose": "1", "COM9-AnswerTimeout": "10000.0000", "528 Shutter-Name": "AnalogIO", "PRIME-Trigger Timeout (secs)": "10", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "NextFrame": 0, "470 AO-TriggerSequenceLength": "1000", "PRIME-TransposeXY": "0", "445 AO-TriggerSequenceLength": "1000", "COM1-Name": "COM1", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "COM9-DataBits": "8", "COM1-AnswerTimeout": "500.0000", "Camera": "", "TE2000-Version": "V2.50-V2.50-V2.10", "528 Shutter-Volts": "0.0000", "XLIGHT Hub-Name": "XLIGHT Hub", "PIEZO-Description": "Ludl stage driver adapter", "PositionName": "Pos17", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSampleRate": "100000", "PRIME-FanSpeedSetpoint": "High", "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "405 AO-TriggerSampleRate": "100000", "470 Shutter-Block voltage": "false", "470-State": "0", "COM3-Parity": "None", "405 AO-Volts": "0.0000", "PRIME-TransposeMirrorY": "0", "PRIME-ChipName": "GS144BSI", "470 AO-Volts": "0.0000", "PRIME-TransposeMirrorX": "0", "445 Shutter-SupportsTriggering": "0", "470 Shutter-SupportsTriggering": "0", "XLIGHT Hub-Description": "XLIGHT hub", "PRIME-PreampOffLimit": "10000", "470 AO-Sequenceable": "1", "528 Shutter-MaxVolts": "5.0000", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "Core-XYStage": "XYStage", "470 AO-TriggerSampleRate": "100000", "PRIME-Timing-PreTriggerDelayNs": "24672000.0000", "528-DA Device": "528 Shutter", "Focus-Description": "Nikon TE2000 focus adapter", "FilterBlock-State": "5", "520 AO-Demo": "No", "COM3-DelayBetweenCharsMs": "0.0000", "470 Shutter-Volts": "0.0000", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "PRIME-PP 2 THRESHOLD": "125", "PRIME-PP 4 THRESHOLD": "75", "XYStage-Acceleration": "4.0000", "470 Shutter-Demo": "No", "PRIME-Name": "PMPCIECam00", "640-DA Device": "640 Shutter", "405 AO-Block voltage": "false", "ElapsedTime-ms": 746170, "PRIME-Offset": "100", "445 AO-IOChannel": "Dev1/ao1", "640 AO-MaxVolts": "5.0000", "PositionIndex": 17, "PRIME-PP 1 ENABLED": "Yes", "COM1-BaudRate": "9600", "640-State": "0", "COM3-Verbose": "1", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "LudlController-TransmissionDelay": "0", "PRIME-FullWellCapacity": "62000", "PRIME-SMARTStreamingEnabled": "No", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "640 Shutter-Description": "NI DAC", "528-Description": "DA device that is used as a shutter", "COM4-Description": "Serial port driver (boost:asio)", "405 Shutter-Description": "NI DAC", "TE2000-Name": "TE2000", "528 Shutter-Description": "NI DAC", "COM9-Description": "Serial port driver (boost:asio)", "LudlController-Name": "LudlController", "445 Shutter-TriggerSequenceLength": "1000", "PRIME-SerialNumber": "A18B203008", "445 AO-Volts": "0.0000", "PRIME-X-dimension": "1200", "PRIME-ShutterCloseDelay": "0", "XLIGHT Disk slider-State": "2", "Position": "Default", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 AO-IOChannel": "Dev1/ao5", "520 AO-IOChannel": "Dev1/ao4", "528 AO-TriggerSequenceLength": "1000", "405 Shutter-MaxVolts": "5.0000", "470-DA Device": "470 Shutter", "PRIME-FTCapable": "No", "Core-ChannelGroup": "Channels", "555 AO-SupportsTriggering": "0", "PRIME-Y-dimension": "1200", "Frame": 0, "555 Shutter-Sequenceable": "1", "PRIME-Timing-ExposureTimeNs": "499998000.0000", "Core-ImageProcessor": "", "528-State": "0", "520 AO-Block voltage": "false", "XLIGHT Excitation Wheel-Label": "Excitation-2", "640 Shutter-Demo": "No", "COM3-StopBits": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "520 Shutter-IOChannel": "Dev1/ao11", "Width": 1200, "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-SupportsTriggering": "0", "640 AO-TriggerSampleRate": "100000", "520-Description": "DA device that is used as a shutter", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "405 Shutter-Sequenceable": "1", "405 Shutter-SupportsTriggering": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-TriggerSampleRate": "100000", "640 Shutter-MaxVolts": "5.0000", "555 AO-MaxVolts": "5.0000", "520 AO-TriggerSequenceLength": "1000", "COM3-BaudRate": "9600", "COM9-BaudRate": "9600", "Vincent-D3-Channel #": "Ch. #1", "PRIME-PMode": "Normal", "OpticalPath-Label": "Left(100)", "BitDepth": 16, "555 AO-TriggerSequenceLength": "1000", "445 Shutter-Description": "NI DAC", "405 AO-Sequenceable": "1", "PRIME-AcquisitionMethod": "Callbacks", "555 AO-Name": "AnalogIO", "PRIME-ShutterMode": "Pre-Exposure", "COM3-Handshaking": "Off", "445 AO-SupportsTriggering": "0", "ROI": "0-0-1200-1200", "COM9-Verbose": "1", "PRIME-Color": "OFF", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "PRIME-ShutterOpenDelay": "0", "COM1-Parity": "None", "Slice": 8, "SlicePosition": 30, "COM9-DelayBetweenCharsMs": "0.0000", "555-Name": "DA Shutter", "PIEZO-LudlSingleAxisName": "B", "XLIGHT Emission Wheel-Label": "Empty", "640 Shutter-Name": "AnalogIO", "640 AO-Sequenceable": "1", "520 Shutter-Demo": "No", "528 Shutter-Demo": "No", "470-Name": "DA Shutter", "520-DA Device": "520 Shutter", "XYStage-Description": "Ludl XY stage driver adapter", "445 Shutter-MinVolts": "0.0000", "528 AO-TriggerSampleRate": "100000", "405 AO-MinVolts": "0.0000", "405 Shutter-TriggerSequenceLength": "1000", "Height": 1200, "555 AO-Block voltage": "false", "640 Shutter-Volts": "0.0000", "PixelType": "GRAY16", "445 Shutter-Block voltage": "false", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "520 Shutter-TriggerSequenceLength": "1000", "COM9-Name": "COM9", "Core-SLM": "", "555 AO-Volts": "0.0000", "555 Shutter-Block voltage": "false", "XYStage-Speed": "1800.0000", "520 AO-MaxVolts": "5.0000", "528 Shutter-SupportsTriggering": "0", "COM1-Description": "Serial port driver (boost:asio)", "PRIME-MetadataEnabled": "No", "520 Shutter-MinVolts": "0.0000", "470 Shutter-Sequenceable": "1", "PRIME-PreampDelay": "0", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-MinVolts": "0.0000", "ChannelIndex": 4, "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Core-AutoShutter": "1", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "Exposure-ms": 20, "PRIME-PP 2 ENABLED": "Yes", "640 Shutter-TriggerSampleRate": "100000", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "470 Shutter-IOChannel": "Dev1/ao10", "405 AO-Demo": "No", "PixelSizeUm": 1.1, "470 AO-Demo": "No", "470 Shutter-MaxVolts": "5.0000", "Vincent-D3-Name": "Vincent-D3", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FileName": "img_000000000_P-DIC_008.tif", "LudlController-Reset": "Operate", "PRIME-Exposure": "20.00", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "445-Name": "DA Shutter", "555 Shutter-Name": "AnalogIO", "520 Shutter-TriggerSampleRate": "100000", "445 Shutter-IOChannel": "Dev1/ao9", "PRIME-TransposeCorrection": "0", "XYStage-StepSize": "0.1000", "445-Description": "DA device that is used as a shutter", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 AO-IOChannel": "Dev1/ao7", "520 Shutter-Name": "AnalogIO", "COM3-DataBits": "8", "640 AO-Volts": "0.0000", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "555 Shutter-IOChannel": "Dev1/ao13", "WaitInterval": null, "520 Shutter-Sequenceable": "1", "YPositionUm": -13012.300000000001, "445-State": "0", "445-DA Device": "445 Shutter", "PRIME-CircularBufferAutoSize": "ON", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "555 AO-TriggerSampleRate": "100000", "445 Shutter-Volts": "0.0000", "XLIGHT Hub-Port": "COM3", "Core-AutoFocus": "", "COM4-Handshaking": "Off", "528 Shutter-MinVolts": "0.0000", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "445 Shutter-Name": "AnalogIO", "528 Shutter-Sequenceable": "1", "XYStage-TransposeMirrorX": "0", "XYStage-TransposeMirrorY": "0", "PRIME-ReadoutRate": "100MHz 16bit", "XYStage-Name": "Stage", "640 AO-SupportsTriggering": "0", "640-Name": "DA Shutter", "640 Shutter-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "640 AO-Block voltage": "false", "445 Shutter-Sequenceable": "1", "470 AO-MaxVolts": "5.0000", "COM4-BaudRate": "9600", "470-Description": "DA device that is used as a shutter", "520 Shutter-Description": "NI DAC", "PRIME-CCDTemperature": "-14.9200", "Time": "2019-12-17 04:00:03 -0500", "640 AO-TriggerSequenceLength": "1000", "470 AO-Name": "AnalogIO", "XLIGHT Disk slider-Label": "Disk Pos 40um", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "Binning": "1x1", "TE2000-ModelType": "1", "COM3-AnswerTimeout": "500.0000", "555 Shutter-SupportsTriggering": "0", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "TE2000-Port": "COM4", "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Description": "NI DAC", "COM4-AnswerTimeout": "500.0000", "COM1-DelayBetweenCharsMs": "0.0000", "FilterBlock-Label": "Confocal", "445 AO-Name": "AnalogIO", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-Timing-ReadoutTimeNs": "24672000.0000", "528 AO-Demo": "No", "520 AO-Sequenceable": "1", "555-Description": "DA device that is used as a shutter", "405-DA Device": "405 Shutter", "405 AO-TriggerSequenceLength": "1000", "640 AO-MinVolts": "0.0000", "640 Shutter-Block voltage": "false", "FrameIndex": 0, "PRIME-Port": "CMOS", "555 AO-MinVolts": "0.0000", "COM3-Description": "Serial port driver (boost:asio)", "528 AO-Description": "NI DAC", "555 AO-IOChannel": "Dev1/ao6", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "555 AO-TriggerInputLine": "/Dev1/PFI0", "PRIME-ActualInterval-ms": "84.6200", "LudlController-Port": "COM9", "445 Shutter-Demo": "No", "PRIME-Trigger-Expose Out-Mux": "1", "SliceIndex": 8, "PRIME-CCDTemperatureSetPoint": "-15.0000", "405 Shutter-MinVolts": "0.0000", "PRIME-ExposeOutMode": "First Row", "AxisPositions": null, "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Volts": "0.0000", "528-Name": "DA Shutter", "555-State": "0", "XLIGHT Dichroic-Label": "Di03-R635-t1", "445 AO-Sequenceable": "1", "405-Name": "DA Shutter", "405 AO-IOChannel": "Dev1/ao0", "Core-Camera": "PRIME", "Focus-Name": "Focus", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "405 Shutter-IOChannel": "Dev1/ao8", "640 AO-Demo": "No", "640 Shutter-SupportsTriggering": "0", "445 AO-Demo": "No", "555-DA Device": "555 Shutter", "470 Shutter-Description": "NI DAC", "528 AO-Volts": "0.0000", "COM1-DataBits": "8", "Vincent-D3-Address": "x", "520 AO-SupportsTriggering": "0", "405-Description": "DA device that is used as a shutter", "640 Shutter-MinVolts": "0.0000", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "405 AO-Name": "AnalogIO", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "640 AO-TriggerInputLine": "/Dev1/PFI0", "405 AO-TriggerInputLine": "/Dev1/PFI0", "640-Description": "DA device that is used as a shutter", "445 Shutter-TriggerSampleRate": "100000", "470 Shutter-TriggerSequenceLength": "1000", "COM1-StopBits": "1", "520 Shutter-SupportsTriggering": "0", "405-State": "0", "Core-Initialize": "1", "XLIGHT Emission Wheel-State": "3", "520 AO-Description": "NI DAC", "528 AO-TriggerInputLine": "/Dev1/PFI0", "445 Shutter-MaxVolts": "5.0000", "COM9-Handshaking": "Off", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "XLIGHT Spin motor-Label": "On (spinning)", "PRIME-PP 5 ENABLED": "No", "UUID": "b89ed6d1-5554-45b3-b120-8e09f1b59de2", "PRIME-TriggerMode": "Internal Trigger", "COM1-Handshaking": "Off", "PRIME-Timing-PostTriggerDelayNs": "49344000.0000", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "Focus-Position": "16732.9000", "528 Shutter-Block voltage": "false", "Channel": "P-DIC", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "555 Shutter-Demo": "No", "405 Shutter-Demo": "No", "528 AO-Name": "AnalogIO", "520-State": "0", "Core-Galvo": "", "405 Shutter-TriggerSampleRate": "100000", "Focus-FocusStepSize_(nm)": "100", "PRIME-PixelType": "16bit", "COM4-DataBits": "8", "470 AO-IOChannel": "Dev1/ao2", "520 AO-MinVolts": "0.0000", "COM9-Parity": "None", "PRIME-CircularBufferEnabled": "ON", "PRIME-PP 6 Reset": "No", "640 AO-Description": "NI DAC", "Source": "PRIME", "445 AO-MinVolts": "0.0000", "PIEZO-Name": "Stage", "445 AO-Description": "NI DAC", "PRIME-CircularBufferFrameCount": "46", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "520 AO-Volts": "0.0000", "528 Shutter-TriggerSampleRate": "100000", "XPositionUm": 23269.100000000002, "OpticalPath-State": "4", "PRIME-PP 3 THRESHOLD": "80", "PRIME-PVCAM Version": "3.7.1", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Gain": "1-HDR", "640 Shutter-TriggerSequenceLength": "1000", "528 AO-SupportsTriggering": "0", "445 AO-TriggerInputLine": "/Dev1/PFI0", "OpticalPath-Name": "OpticalPath", "PRIME-PP 4 ENABLED": "Yes", "PRIME-PVCAM Adapter Version": "1.3.29", "COM3-Name": "COM3", "405 AO-MaxVolts": "5.0000", "520 Shutter-Block voltage": "false", "PRIME-Timing-ClearingTimeNs": "49344000.0000", "Core-Focus": "PIEZO", "528 AO-Block voltage": "false", "TE2000-Description": "Nikon TE2000 hub", "528 AO-MinVolts": "0.0000", "COM4-Parity": "None", "Summary": { "Slices": 11, "ChContrastMin": [ 0, 0, 0, 0, 0, 0 ], "PixelAspect": 1, "SlicesFirst": true, "Interval_ms": 0, "Time": "2019-12-17 03:59:33 -0500", "Prefix": "seq_7", "InitialPositionList": [ { "Label": "Pos0", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [149.91], "Focus": [16657.7], "XYStage": [ 71629.7, 19152.9 ] }, "GridColumnIndex": 0 }, { "Label": "Pos1", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [190.82], "Focus": [16485.4], "XYStage": [ 65702.3, 19396.600000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos2", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [194.69], "Focus": [16446.8], "XYStage": [ 59880.5, 19007.8 ] }, "GridColumnIndex": 0 }, { "Label": "Pos3", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [145.04], "Focus": [16446.6], "XYStage": [ 53616.9, 18818.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos4", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [169.57500000000002], "Focus": [16446.5], "XYStage": [ 47662.8, 18817.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos5", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [131.805], "Focus": [16446.4], "XYStage": [ 41918.8, 18298.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos6", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [113.145], "Focus": [16446.5], "XYStage": [ 36129.9, 18895.8 ] }, "GridColumnIndex": 0 }, { "Label": "Pos7", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [141.005], "Focus": [16446.6], "XYStage": [ 30299.100000000002, 18895.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos8", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [140.97], "Focus": [16446.5], "XYStage": [ 23952.100000000002, 19156 ] }, "GridColumnIndex": 0 }, { "Label": "Pos9", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [184.57500000000002], "Focus": [16677.9], "XYStage": [ 72866.8, -14809.300000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos10", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [167.95000000000002], "Focus": [16557.9], "XYStage": [ 66515.6, -14371.300000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos11", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [168], "Focus": [16618.9], "XYStage": [ 59724.3, -13964.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos12", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [203.205], "Focus": [16779.6], "XYStage": [ 53862.3, -13494.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos13", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [203.20000000000002], "Focus": [16749], "XYStage": [ 48108.3, -13805.400000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos14", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [203.19], "Focus": [16675.9], "XYStage": [ 41645, -13804.900000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos15", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [203.21], "Focus": [16675.7], "XYStage": [ 35352.6, -13805.6 ] }, "GridColumnIndex": 0 }, { "Label": "Pos16", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [203.225], "Focus": [16675.8], "XYStage": [ 29298, -13447.400000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos17", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [203.215], "Focus": [16675.8], "XYStage": [ 23269.100000000002, -13012.300000000001 ] }, "GridColumnIndex": 0 } ], "z-step_um": 10, "GridRow": 0, "BitDepth": 16, "Depth": 2, "Channels": 6, "Source": "Micro-Manager", "ChColors": [ -16711681, -256, -65281, -1, -1, -16776961 ], "MetadataVersion": 10, "KeepShutterOpenChannels": true, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536, 65536 ], "CustomIntervals_ms": [], "Height": 1200, "MicroManagerVersion": "1.4.23 20180219", "GridColumn": 0, "UUID": "3ad8801b-5699-40d6-813b-4ad840863e38", "PixelType": "GRAY16", "Width": 1200, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\Yan\\20191216-YC113-S11S12-miseq", "Frames": 1, "Comment": "", "UserName": "Nikon", "PixelSize_um": 1.1, "ROI": [ 0, 0, 1200, 1200 ], "Date": "2019-12-17", "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC", "P-DAPI-high" ], "KeepShutterOpenSlices": true, "IJType": 1, "ComputerName": "ZadorConfocal2", "Positions": 18, "PositionIndex": 17, "CameraTimeout": 20000, "TimeFirst": false }, "640 AO-Name": "AnalogIO", "Vincent-D3-Command": "Close" }, "FrameKey-0-4-9": { "COM4-StopBits": "1", "528 AO-MaxVolts": "5.0000", "XLIGHT Excitation Wheel-State": "1", "470 Shutter-Name": "AnalogIO", "Core-Shutter": "Vincent-D3", "555 AO-Demo": "No", "XLIGHT Dichroic-State": "2", "XYStage-StepSize-Y": "0.1000", "ZPositionUm": 243.215, "XYStage-StepSize-X": "0.1000", "PRIME-FirmwareVersion": "20.28", "PRIME-ClearCycles": "2", "520-Name": "DA Shutter", "405 Shutter-Name": "AnalogIO", "PIEZO-StepSize": "0.0050", "PRIME-ClearMode": "Pre-Exposure", "470 AO-MinVolts": "0.0000", "405 AO-SupportsTriggering": "1", "CameraChannelIndex": 0, "XYStage-StartSpeed": "500.0000", "COM9-StopBits": "2", "405 AO-Description": "NI DAC", "COM4-DelayBetweenCharsMs": "0.0000", "445 AO-Block voltage": "false", "520 AO-TriggerInputLine": "/Dev1/PFI0", "555 AO-Description": "NI DAC", "520 AO-TriggerSampleRate": "100000", "COM4-Verbose": "1", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM4-Name": "COM4", "405 Shutter-Volts": "0.0000", "Vincent-D3-Port": "COM1", "PRIME-PP 4 MIN ADU AFFECTED": "200", "470 Shutter-MinVolts": "0.0000", "PRIME-PP 3 ENABLED": "Yes", "470 AO-Description": "NI DAC", "PRIME-Description": "PVCAM API device adapter", "520 Shutter-MaxVolts": "5.0000", "520 Shutter-Volts": "0.0000", "528 AO-Sequenceable": "1", "PRIME-Binning": "1x1", "555 Shutter-MaxVolts": "5.0000", "528 Shutter-TriggerSequenceLength": "1000", "405 Shutter-Block voltage": "false", "XLIGHT Spin motor-State": "1", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "Vincent-D3-Time to open (ms)": "35.0000", "Vincent-D3-Time to close (ms)": "35.0000", "445 AO-TriggerSampleRate": "100000", "640 Shutter-IOChannel": "Dev1/ao14", "555 AO-Sequenceable": "1", "520 AO-Name": "AnalogIO", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "470 AO-Block voltage": "false", "COM1-Verbose": "1", "COM9-AnswerTimeout": "10000.0000", "528 Shutter-Name": "AnalogIO", "PRIME-Trigger Timeout (secs)": "10", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "NextFrame": 0, "470 AO-TriggerSequenceLength": "1000", "PRIME-TransposeXY": "0", "445 AO-TriggerSequenceLength": "1000", "COM1-Name": "COM1", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "COM9-DataBits": "8", "COM1-AnswerTimeout": "500.0000", "Camera": "", "TE2000-Version": "V2.50-V2.50-V2.10", "528 Shutter-Volts": "0.0000", "XLIGHT Hub-Name": "XLIGHT Hub", "PIEZO-Description": "Ludl stage driver adapter", "PositionName": "Pos17", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSampleRate": "100000", "PRIME-FanSpeedSetpoint": "High", "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "405 AO-TriggerSampleRate": "100000", "470 Shutter-Block voltage": "false", "470-State": "0", "COM3-Parity": "None", "405 AO-Volts": "0.0000", "PRIME-TransposeMirrorY": "0", "PRIME-ChipName": "GS144BSI", "470 AO-Volts": "0.0000", "PRIME-TransposeMirrorX": "0", "445 Shutter-SupportsTriggering": "0", "470 Shutter-SupportsTriggering": "0", "XLIGHT Hub-Description": "XLIGHT hub", "PRIME-PreampOffLimit": "10000", "470 AO-Sequenceable": "1", "528 Shutter-MaxVolts": "5.0000", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "Core-XYStage": "XYStage", "470 AO-TriggerSampleRate": "100000", "PRIME-Timing-PreTriggerDelayNs": "24672000.0000", "528-DA Device": "528 Shutter", "Focus-Description": "Nikon TE2000 focus adapter", "FilterBlock-State": "5", "520 AO-Demo": "No", "COM3-DelayBetweenCharsMs": "0.0000", "470 Shutter-Volts": "0.0000", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "PRIME-PP 2 THRESHOLD": "125", "PRIME-PP 4 THRESHOLD": "75", "XYStage-Acceleration": "4.0000", "470 Shutter-Demo": "No", "PRIME-Name": "PMPCIECam00", "640-DA Device": "640 Shutter", "405 AO-Block voltage": "false", "ElapsedTime-ms": 746476, "PRIME-Offset": "100", "445 AO-IOChannel": "Dev1/ao1", "640 AO-MaxVolts": "5.0000", "PositionIndex": 17, "PRIME-PP 1 ENABLED": "Yes", "COM1-BaudRate": "9600", "640-State": "0", "COM3-Verbose": "1", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "LudlController-TransmissionDelay": "0", "PRIME-FullWellCapacity": "62000", "PRIME-SMARTStreamingEnabled": "No", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "640 Shutter-Description": "NI DAC", "528-Description": "DA device that is used as a shutter", "COM4-Description": "Serial port driver (boost:asio)", "405 Shutter-Description": "NI DAC", "TE2000-Name": "TE2000", "528 Shutter-Description": "NI DAC", "COM9-Description": "Serial port driver (boost:asio)", "LudlController-Name": "LudlController", "445 Shutter-TriggerSequenceLength": "1000", "PRIME-SerialNumber": "A18B203008", "445 AO-Volts": "0.0000", "PRIME-X-dimension": "1200", "PRIME-ShutterCloseDelay": "0", "XLIGHT Disk slider-State": "2", "Position": "Default", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 AO-IOChannel": "Dev1/ao5", "520 AO-IOChannel": "Dev1/ao4", "528 AO-TriggerSequenceLength": "1000", "405 Shutter-MaxVolts": "5.0000", "470-DA Device": "470 Shutter", "PRIME-FTCapable": "No", "Core-ChannelGroup": "Channels", "555 AO-SupportsTriggering": "0", "PRIME-Y-dimension": "1200", "Frame": 0, "555 Shutter-Sequenceable": "1", "PRIME-Timing-ExposureTimeNs": "499998000.0000", "Core-ImageProcessor": "", "528-State": "0", "520 AO-Block voltage": "false", "XLIGHT Excitation Wheel-Label": "Excitation-2", "640 Shutter-Demo": "No", "COM3-StopBits": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "520 Shutter-IOChannel": "Dev1/ao11", "Width": 1200, "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-SupportsTriggering": "0", "640 AO-TriggerSampleRate": "100000", "520-Description": "DA device that is used as a shutter", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "405 Shutter-Sequenceable": "1", "405 Shutter-SupportsTriggering": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-TriggerSampleRate": "100000", "640 Shutter-MaxVolts": "5.0000", "555 AO-MaxVolts": "5.0000", "520 AO-TriggerSequenceLength": "1000", "COM3-BaudRate": "9600", "COM9-BaudRate": "9600", "Vincent-D3-Channel #": "Ch. #1", "PRIME-PMode": "Normal", "OpticalPath-Label": "Left(100)", "BitDepth": 16, "555 AO-TriggerSequenceLength": "1000", "445 Shutter-Description": "NI DAC", "405 AO-Sequenceable": "1", "PRIME-AcquisitionMethod": "Callbacks", "555 AO-Name": "AnalogIO", "PRIME-ShutterMode": "Pre-Exposure", "COM3-Handshaking": "Off", "445 AO-SupportsTriggering": "0", "ROI": "0-0-1200-1200", "COM9-Verbose": "1", "PRIME-Color": "OFF", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "PRIME-ShutterOpenDelay": "0", "COM1-Parity": "None", "Slice": 9, "SlicePosition": 40, "COM9-DelayBetweenCharsMs": "0.0000", "555-Name": "DA Shutter", "PIEZO-LudlSingleAxisName": "B", "XLIGHT Emission Wheel-Label": "Empty", "640 Shutter-Name": "AnalogIO", "640 AO-Sequenceable": "1", "520 Shutter-Demo": "No", "528 Shutter-Demo": "No", "470-Name": "DA Shutter", "520-DA Device": "520 Shutter", "XYStage-Description": "Ludl XY stage driver adapter", "445 Shutter-MinVolts": "0.0000", "528 AO-TriggerSampleRate": "100000", "405 AO-MinVolts": "0.0000", "405 Shutter-TriggerSequenceLength": "1000", "Height": 1200, "555 AO-Block voltage": "false", "640 Shutter-Volts": "0.0000", "PixelType": "GRAY16", "445 Shutter-Block voltage": "false", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "520 Shutter-TriggerSequenceLength": "1000", "COM9-Name": "COM9", "Core-SLM": "", "555 AO-Volts": "0.0000", "555 Shutter-Block voltage": "false", "XYStage-Speed": "1800.0000", "520 AO-MaxVolts": "5.0000", "528 Shutter-SupportsTriggering": "0", "COM1-Description": "Serial port driver (boost:asio)", "PRIME-MetadataEnabled": "No", "520 Shutter-MinVolts": "0.0000", "470 Shutter-Sequenceable": "1", "PRIME-PreampDelay": "0", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-MinVolts": "0.0000", "ChannelIndex": 4, "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Core-AutoShutter": "1", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "Exposure-ms": 20, "PRIME-PP 2 ENABLED": "Yes", "640 Shutter-TriggerSampleRate": "100000", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "470 Shutter-IOChannel": "Dev1/ao10", "405 AO-Demo": "No", "PixelSizeUm": 1.1, "470 AO-Demo": "No", "470 Shutter-MaxVolts": "5.0000", "Vincent-D3-Name": "Vincent-D3", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FileName": "img_000000000_P-DIC_009.tif", "LudlController-Reset": "Operate", "PRIME-Exposure": "20.00", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "445-Name": "DA Shutter", "555 Shutter-Name": "AnalogIO", "520 Shutter-TriggerSampleRate": "100000", "445 Shutter-IOChannel": "Dev1/ao9", "PRIME-TransposeCorrection": "0", "XYStage-StepSize": "0.1000", "445-Description": "DA device that is used as a shutter", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 AO-IOChannel": "Dev1/ao7", "520 Shutter-Name": "AnalogIO", "COM3-DataBits": "8", "640 AO-Volts": "0.0000", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "555 Shutter-IOChannel": "Dev1/ao13", "WaitInterval": null, "520 Shutter-Sequenceable": "1", "YPositionUm": -13012.300000000001, "445-State": "0", "445-DA Device": "445 Shutter", "PRIME-CircularBufferAutoSize": "ON", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "555 AO-TriggerSampleRate": "100000", "445 Shutter-Volts": "0.0000", "XLIGHT Hub-Port": "COM3", "Core-AutoFocus": "", "COM4-Handshaking": "Off", "528 Shutter-MinVolts": "0.0000", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "445 Shutter-Name": "AnalogIO", "528 Shutter-Sequenceable": "1", "XYStage-TransposeMirrorX": "0", "XYStage-TransposeMirrorY": "0", "PRIME-ReadoutRate": "100MHz 16bit", "XYStage-Name": "Stage", "640 AO-SupportsTriggering": "0", "640-Name": "DA Shutter", "640 Shutter-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "640 AO-Block voltage": "false", "445 Shutter-Sequenceable": "1", "470 AO-MaxVolts": "5.0000", "COM4-BaudRate": "9600", "470-Description": "DA device that is used as a shutter", "520 Shutter-Description": "NI DAC", "PRIME-CCDTemperature": "-14.9200", "Time": "2019-12-17 04:00:03 -0500", "640 AO-TriggerSequenceLength": "1000", "470 AO-Name": "AnalogIO", "XLIGHT Disk slider-Label": "Disk Pos 40um", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "Binning": "1x1", "TE2000-ModelType": "1", "COM3-AnswerTimeout": "500.0000", "555 Shutter-SupportsTriggering": "0", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "TE2000-Port": "COM4", "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Description": "NI DAC", "COM4-AnswerTimeout": "500.0000", "COM1-DelayBetweenCharsMs": "0.0000", "FilterBlock-Label": "Confocal", "445 AO-Name": "AnalogIO", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-Timing-ReadoutTimeNs": "24672000.0000", "528 AO-Demo": "No", "520 AO-Sequenceable": "1", "555-Description": "DA device that is used as a shutter", "405-DA Device": "405 Shutter", "405 AO-TriggerSequenceLength": "1000", "640 AO-MinVolts": "0.0000", "640 Shutter-Block voltage": "false", "FrameIndex": 0, "PRIME-Port": "CMOS", "555 AO-MinVolts": "0.0000", "COM3-Description": "Serial port driver (boost:asio)", "528 AO-Description": "NI DAC", "555 AO-IOChannel": "Dev1/ao6", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "555 AO-TriggerInputLine": "/Dev1/PFI0", "PRIME-ActualInterval-ms": "84.6200", "LudlController-Port": "COM9", "445 Shutter-Demo": "No", "PRIME-Trigger-Expose Out-Mux": "1", "SliceIndex": 9, "PRIME-CCDTemperatureSetPoint": "-15.0000", "405 Shutter-MinVolts": "0.0000", "PRIME-ExposeOutMode": "First Row", "AxisPositions": null, "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Volts": "0.0000", "528-Name": "DA Shutter", "555-State": "0", "XLIGHT Dichroic-Label": "Di03-R635-t1", "445 AO-Sequenceable": "1", "405-Name": "DA Shutter", "405 AO-IOChannel": "Dev1/ao0", "Core-Camera": "PRIME", "Focus-Name": "Focus", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "405 Shutter-IOChannel": "Dev1/ao8", "640 AO-Demo": "No", "640 Shutter-SupportsTriggering": "0", "445 AO-Demo": "No", "555-DA Device": "555 Shutter", "470 Shutter-Description": "NI DAC", "528 AO-Volts": "0.0000", "COM1-DataBits": "8", "Vincent-D3-Address": "x", "520 AO-SupportsTriggering": "0", "405-Description": "DA device that is used as a shutter", "640 Shutter-MinVolts": "0.0000", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "405 AO-Name": "AnalogIO", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "640 AO-TriggerInputLine": "/Dev1/PFI0", "405 AO-TriggerInputLine": "/Dev1/PFI0", "640-Description": "DA device that is used as a shutter", "445 Shutter-TriggerSampleRate": "100000", "470 Shutter-TriggerSequenceLength": "1000", "COM1-StopBits": "1", "520 Shutter-SupportsTriggering": "0", "405-State": "0", "Core-Initialize": "1", "XLIGHT Emission Wheel-State": "3", "520 AO-Description": "NI DAC", "528 AO-TriggerInputLine": "/Dev1/PFI0", "445 Shutter-MaxVolts": "5.0000", "COM9-Handshaking": "Off", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "XLIGHT Spin motor-Label": "On (spinning)", "PRIME-PP 5 ENABLED": "No", "UUID": "556aee53-1f6f-4274-b04e-2c91569773b5", "PRIME-TriggerMode": "Internal Trigger", "COM1-Handshaking": "Off", "PRIME-Timing-PostTriggerDelayNs": "49344000.0000", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "Focus-Position": "16732.9000", "528 Shutter-Block voltage": "false", "Channel": "P-DIC", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "555 Shutter-Demo": "No", "405 Shutter-Demo": "No", "528 AO-Name": "AnalogIO", "520-State": "0", "Core-Galvo": "", "405 Shutter-TriggerSampleRate": "100000", "Focus-FocusStepSize_(nm)": "100", "PRIME-PixelType": "16bit", "COM4-DataBits": "8", "470 AO-IOChannel": "Dev1/ao2", "520 AO-MinVolts": "0.0000", "COM9-Parity": "None", "PRIME-CircularBufferEnabled": "ON", "PRIME-PP 6 Reset": "No", "640 AO-Description": "NI DAC", "Source": "PRIME", "445 AO-MinVolts": "0.0000", "PIEZO-Name": "Stage", "445 AO-Description": "NI DAC", "PRIME-CircularBufferFrameCount": "46", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "520 AO-Volts": "0.0000", "528 Shutter-TriggerSampleRate": "100000", "XPositionUm": 23269.100000000002, "OpticalPath-State": "4", "PRIME-PP 3 THRESHOLD": "80", "PRIME-PVCAM Version": "3.7.1", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Gain": "1-HDR", "640 Shutter-TriggerSequenceLength": "1000", "528 AO-SupportsTriggering": "0", "445 AO-TriggerInputLine": "/Dev1/PFI0", "OpticalPath-Name": "OpticalPath", "PRIME-PP 4 ENABLED": "Yes", "PRIME-PVCAM Adapter Version": "1.3.29", "COM3-Name": "COM3", "405 AO-MaxVolts": "5.0000", "520 Shutter-Block voltage": "false", "PRIME-Timing-ClearingTimeNs": "49344000.0000", "Core-Focus": "PIEZO", "528 AO-Block voltage": "false", "TE2000-Description": "Nikon TE2000 hub", "528 AO-MinVolts": "0.0000", "COM4-Parity": "None", "Summary": { "Slices": 11, "ChContrastMin": [ 0, 0, 0, 0, 0, 0 ], "PixelAspect": 1, "SlicesFirst": true, "Interval_ms": 0, "Time": "2019-12-17 03:59:33 -0500", "Prefix": "seq_7", "InitialPositionList": [ { "Label": "Pos0", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [149.91], "Focus": [16657.7], "XYStage": [ 71629.7, 19152.9 ] }, "GridColumnIndex": 0 }, { "Label": "Pos1", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [190.82], "Focus": [16485.4], "XYStage": [ 65702.3, 19396.600000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos2", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [194.69], "Focus": [16446.8], "XYStage": [ 59880.5, 19007.8 ] }, "GridColumnIndex": 0 }, { "Label": "Pos3", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [145.04], "Focus": [16446.6], "XYStage": [ 53616.9, 18818.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos4", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [169.57500000000002], "Focus": [16446.5], "XYStage": [ 47662.8, 18817.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos5", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [131.805], "Focus": [16446.4], "XYStage": [ 41918.8, 18298.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos6", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [113.145], "Focus": [16446.5], "XYStage": [ 36129.9, 18895.8 ] }, "GridColumnIndex": 0 }, { "Label": "Pos7", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [141.005], "Focus": [16446.6], "XYStage": [ 30299.100000000002, 18895.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos8", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [140.97], "Focus": [16446.5], "XYStage": [ 23952.100000000002, 19156 ] }, "GridColumnIndex": 0 }, { "Label": "Pos9", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [184.57500000000002], "Focus": [16677.9], "XYStage": [ 72866.8, -14809.300000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos10", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [167.95000000000002], "Focus": [16557.9], "XYStage": [ 66515.6, -14371.300000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos11", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [168], "Focus": [16618.9], "XYStage": [ 59724.3, -13964.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos12", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [203.205], "Focus": [16779.6], "XYStage": [ 53862.3, -13494.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos13", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [203.20000000000002], "Focus": [16749], "XYStage": [ 48108.3, -13805.400000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos14", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [203.19], "Focus": [16675.9], "XYStage": [ 41645, -13804.900000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos15", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [203.21], "Focus": [16675.7], "XYStage": [ 35352.6, -13805.6 ] }, "GridColumnIndex": 0 }, { "Label": "Pos16", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [203.225], "Focus": [16675.8], "XYStage": [ 29298, -13447.400000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos17", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [203.215], "Focus": [16675.8], "XYStage": [ 23269.100000000002, -13012.300000000001 ] }, "GridColumnIndex": 0 } ], "z-step_um": 10, "GridRow": 0, "BitDepth": 16, "Depth": 2, "Channels": 6, "Source": "Micro-Manager", "ChColors": [ -16711681, -256, -65281, -1, -1, -16776961 ], "MetadataVersion": 10, "KeepShutterOpenChannels": true, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536, 65536 ], "CustomIntervals_ms": [], "Height": 1200, "MicroManagerVersion": "1.4.23 20180219", "GridColumn": 0, "UUID": "3ad8801b-5699-40d6-813b-4ad840863e38", "PixelType": "GRAY16", "Width": 1200, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\Yan\\20191216-YC113-S11S12-miseq", "Frames": 1, "Comment": "", "UserName": "Nikon", "PixelSize_um": 1.1, "ROI": [ 0, 0, 1200, 1200 ], "Date": "2019-12-17", "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC", "P-DAPI-high" ], "KeepShutterOpenSlices": true, "IJType": 1, "ComputerName": "ZadorConfocal2", "Positions": 18, "PositionIndex": 17, "CameraTimeout": 20000, "TimeFirst": false }, "640 AO-Name": "AnalogIO", "Vincent-D3-Command": "Close" }, "FrameKey-0-4-10": { "COM4-StopBits": "1", "528 AO-MaxVolts": "5.0000", "XLIGHT Excitation Wheel-State": "1", "470 Shutter-Name": "AnalogIO", "Core-Shutter": "Vincent-D3", "555 AO-Demo": "No", "XLIGHT Dichroic-State": "2", "XYStage-StepSize-Y": "0.1000", "ZPositionUm": 253.215, "XYStage-StepSize-X": "0.1000", "PRIME-FirmwareVersion": "20.28", "PRIME-ClearCycles": "2", "520-Name": "DA Shutter", "405 Shutter-Name": "AnalogIO", "PIEZO-StepSize": "0.0050", "PRIME-ClearMode": "Pre-Exposure", "470 AO-MinVolts": "0.0000", "405 AO-SupportsTriggering": "1", "CameraChannelIndex": 0, "XYStage-StartSpeed": "500.0000", "COM9-StopBits": "2", "405 AO-Description": "NI DAC", "COM4-DelayBetweenCharsMs": "0.0000", "445 AO-Block voltage": "false", "520 AO-TriggerInputLine": "/Dev1/PFI0", "555 AO-Description": "NI DAC", "520 AO-TriggerSampleRate": "100000", "COM4-Verbose": "1", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM4-Name": "COM4", "405 Shutter-Volts": "0.0000", "Vincent-D3-Port": "COM1", "PRIME-PP 4 MIN ADU AFFECTED": "200", "470 Shutter-MinVolts": "0.0000", "PRIME-PP 3 ENABLED": "Yes", "470 AO-Description": "NI DAC", "PRIME-Description": "PVCAM API device adapter", "520 Shutter-MaxVolts": "5.0000", "520 Shutter-Volts": "0.0000", "528 AO-Sequenceable": "1", "PRIME-Binning": "1x1", "555 Shutter-MaxVolts": "5.0000", "528 Shutter-TriggerSequenceLength": "1000", "405 Shutter-Block voltage": "false", "XLIGHT Spin motor-State": "1", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "Vincent-D3-Time to open (ms)": "35.0000", "Vincent-D3-Time to close (ms)": "35.0000", "445 AO-TriggerSampleRate": "100000", "640 Shutter-IOChannel": "Dev1/ao14", "555 AO-Sequenceable": "1", "520 AO-Name": "AnalogIO", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "470 AO-Block voltage": "false", "COM1-Verbose": "1", "COM9-AnswerTimeout": "10000.0000", "528 Shutter-Name": "AnalogIO", "PRIME-Trigger Timeout (secs)": "10", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "NextFrame": 0, "470 AO-TriggerSequenceLength": "1000", "PRIME-TransposeXY": "0", "445 AO-TriggerSequenceLength": "1000", "COM1-Name": "COM1", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "COM9-DataBits": "8", "COM1-AnswerTimeout": "500.0000", "Camera": "", "TE2000-Version": "V2.50-V2.50-V2.10", "528 Shutter-Volts": "0.0000", "XLIGHT Hub-Name": "XLIGHT Hub", "PIEZO-Description": "Ludl stage driver adapter", "PositionName": "Pos17", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSampleRate": "100000", "PRIME-FanSpeedSetpoint": "High", "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "405 AO-TriggerSampleRate": "100000", "470 Shutter-Block voltage": "false", "470-State": "0", "COM3-Parity": "None", "405 AO-Volts": "0.0000", "PRIME-TransposeMirrorY": "0", "PRIME-ChipName": "GS144BSI", "470 AO-Volts": "0.0000", "PRIME-TransposeMirrorX": "0", "445 Shutter-SupportsTriggering": "0", "470 Shutter-SupportsTriggering": "0", "XLIGHT Hub-Description": "XLIGHT hub", "PRIME-PreampOffLimit": "10000", "470 AO-Sequenceable": "1", "528 Shutter-MaxVolts": "5.0000", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "Core-XYStage": "XYStage", "470 AO-TriggerSampleRate": "100000", "PRIME-Timing-PreTriggerDelayNs": "24672000.0000", "528-DA Device": "528 Shutter", "Focus-Description": "Nikon TE2000 focus adapter", "FilterBlock-State": "5", "520 AO-Demo": "No", "COM3-DelayBetweenCharsMs": "0.0000", "470 Shutter-Volts": "0.0000", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "PRIME-PP 2 THRESHOLD": "125", "PRIME-PP 4 THRESHOLD": "75", "XYStage-Acceleration": "4.0000", "470 Shutter-Demo": "No", "PRIME-Name": "PMPCIECam00", "640-DA Device": "640 Shutter", "405 AO-Block voltage": "false", "ElapsedTime-ms": 746747, "PRIME-Offset": "100", "445 AO-IOChannel": "Dev1/ao1", "640 AO-MaxVolts": "5.0000", "PositionIndex": 17, "PRIME-PP 1 ENABLED": "Yes", "COM1-BaudRate": "9600", "640-State": "0", "COM3-Verbose": "1", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "LudlController-TransmissionDelay": "0", "PRIME-FullWellCapacity": "62000", "PRIME-SMARTStreamingEnabled": "No", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "640 Shutter-Description": "NI DAC", "528-Description": "DA device that is used as a shutter", "COM4-Description": "Serial port driver (boost:asio)", "405 Shutter-Description": "NI DAC", "TE2000-Name": "TE2000", "528 Shutter-Description": "NI DAC", "COM9-Description": "Serial port driver (boost:asio)", "LudlController-Name": "LudlController", "445 Shutter-TriggerSequenceLength": "1000", "PRIME-SerialNumber": "A18B203008", "445 AO-Volts": "0.0000", "PRIME-X-dimension": "1200", "PRIME-ShutterCloseDelay": "0", "XLIGHT Disk slider-State": "2", "Position": "Default", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 AO-IOChannel": "Dev1/ao5", "520 AO-IOChannel": "Dev1/ao4", "528 AO-TriggerSequenceLength": "1000", "405 Shutter-MaxVolts": "5.0000", "470-DA Device": "470 Shutter", "PRIME-FTCapable": "No", "Core-ChannelGroup": "Channels", "555 AO-SupportsTriggering": "0", "PRIME-Y-dimension": "1200", "Frame": 0, "555 Shutter-Sequenceable": "1", "PRIME-Timing-ExposureTimeNs": "499998000.0000", "Core-ImageProcessor": "", "528-State": "0", "520 AO-Block voltage": "false", "XLIGHT Excitation Wheel-Label": "Excitation-2", "640 Shutter-Demo": "No", "COM3-StopBits": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "520 Shutter-IOChannel": "Dev1/ao11", "Width": 1200, "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-SupportsTriggering": "0", "640 AO-TriggerSampleRate": "100000", "520-Description": "DA device that is used as a shutter", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "405 Shutter-Sequenceable": "1", "405 Shutter-SupportsTriggering": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-TriggerSampleRate": "100000", "640 Shutter-MaxVolts": "5.0000", "555 AO-MaxVolts": "5.0000", "520 AO-TriggerSequenceLength": "1000", "COM3-BaudRate": "9600", "COM9-BaudRate": "9600", "Vincent-D3-Channel #": "Ch. #1", "PRIME-PMode": "Normal", "OpticalPath-Label": "Left(100)", "BitDepth": 16, "555 AO-TriggerSequenceLength": "1000", "445 Shutter-Description": "NI DAC", "405 AO-Sequenceable": "1", "PRIME-AcquisitionMethod": "Callbacks", "555 AO-Name": "AnalogIO", "PRIME-ShutterMode": "Pre-Exposure", "COM3-Handshaking": "Off", "445 AO-SupportsTriggering": "0", "ROI": "0-0-1200-1200", "COM9-Verbose": "1", "PRIME-Color": "OFF", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "PRIME-ShutterOpenDelay": "0", "COM1-Parity": "None", "Slice": 10, "SlicePosition": 50, "COM9-DelayBetweenCharsMs": "0.0000", "555-Name": "DA Shutter", "PIEZO-LudlSingleAxisName": "B", "XLIGHT Emission Wheel-Label": "Empty", "640 Shutter-Name": "AnalogIO", "640 AO-Sequenceable": "1", "520 Shutter-Demo": "No", "528 Shutter-Demo": "No", "470-Name": "DA Shutter", "520-DA Device": "520 Shutter", "XYStage-Description": "Ludl XY stage driver adapter", "445 Shutter-MinVolts": "0.0000", "528 AO-TriggerSampleRate": "100000", "405 AO-MinVolts": "0.0000", "405 Shutter-TriggerSequenceLength": "1000", "Height": 1200, "555 AO-Block voltage": "false", "640 Shutter-Volts": "0.0000", "PixelType": "GRAY16", "445 Shutter-Block voltage": "false", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "520 Shutter-TriggerSequenceLength": "1000", "COM9-Name": "COM9", "Core-SLM": "", "555 AO-Volts": "0.0000", "555 Shutter-Block voltage": "false", "XYStage-Speed": "1800.0000", "520 AO-MaxVolts": "5.0000", "528 Shutter-SupportsTriggering": "0", "COM1-Description": "Serial port driver (boost:asio)", "PRIME-MetadataEnabled": "No", "520 Shutter-MinVolts": "0.0000", "470 Shutter-Sequenceable": "1", "PRIME-PreampDelay": "0", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-MinVolts": "0.0000", "ChannelIndex": 4, "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Core-AutoShutter": "1", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "Exposure-ms": 20, "PRIME-PP 2 ENABLED": "Yes", "640 Shutter-TriggerSampleRate": "100000", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "470 Shutter-IOChannel": "Dev1/ao10", "405 AO-Demo": "No", "PixelSizeUm": 1.1, "470 AO-Demo": "No", "470 Shutter-MaxVolts": "5.0000", "Vincent-D3-Name": "Vincent-D3", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FileName": "img_000000000_P-DIC_010.tif", "LudlController-Reset": "Operate", "PRIME-Exposure": "20.00", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "445-Name": "DA Shutter", "555 Shutter-Name": "AnalogIO", "520 Shutter-TriggerSampleRate": "100000", "445 Shutter-IOChannel": "Dev1/ao9", "PRIME-TransposeCorrection": "0", "XYStage-StepSize": "0.1000", "445-Description": "DA device that is used as a shutter", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 AO-IOChannel": "Dev1/ao7", "520 Shutter-Name": "AnalogIO", "COM3-DataBits": "8", "640 AO-Volts": "0.0000", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "555 Shutter-IOChannel": "Dev1/ao13", "WaitInterval": null, "520 Shutter-Sequenceable": "1", "YPositionUm": -13012.300000000001, "445-State": "0", "445-DA Device": "445 Shutter", "PRIME-CircularBufferAutoSize": "ON", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "555 AO-TriggerSampleRate": "100000", "445 Shutter-Volts": "0.0000", "XLIGHT Hub-Port": "COM3", "Core-AutoFocus": "", "COM4-Handshaking": "Off", "528 Shutter-MinVolts": "0.0000", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "445 Shutter-Name": "AnalogIO", "528 Shutter-Sequenceable": "1", "XYStage-TransposeMirrorX": "0", "XYStage-TransposeMirrorY": "0", "PRIME-ReadoutRate": "100MHz 16bit", "XYStage-Name": "Stage", "640 AO-SupportsTriggering": "0", "640-Name": "DA Shutter", "640 Shutter-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "640 AO-Block voltage": "false", "445 Shutter-Sequenceable": "1", "470 AO-MaxVolts": "5.0000", "COM4-BaudRate": "9600", "470-Description": "DA device that is used as a shutter", "520 Shutter-Description": "NI DAC", "PRIME-CCDTemperature": "-14.9200", "Time": "2019-12-17 04:00:03 -0500", "640 AO-TriggerSequenceLength": "1000", "470 AO-Name": "AnalogIO", "XLIGHT Disk slider-Label": "Disk Pos 40um", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "Binning": "1x1", "TE2000-ModelType": "1", "COM3-AnswerTimeout": "500.0000", "555 Shutter-SupportsTriggering": "0", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "TE2000-Port": "COM4", "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Description": "NI DAC", "COM4-AnswerTimeout": "500.0000", "COM1-DelayBetweenCharsMs": "0.0000", "FilterBlock-Label": "Confocal", "445 AO-Name": "AnalogIO", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-Timing-ReadoutTimeNs": "24672000.0000", "528 AO-Demo": "No", "520 AO-Sequenceable": "1", "555-Description": "DA device that is used as a shutter", "405-DA Device": "405 Shutter", "405 AO-TriggerSequenceLength": "1000", "640 AO-MinVolts": "0.0000", "640 Shutter-Block voltage": "false", "FrameIndex": 0, "PRIME-Port": "CMOS", "555 AO-MinVolts": "0.0000", "COM3-Description": "Serial port driver (boost:asio)", "528 AO-Description": "NI DAC", "555 AO-IOChannel": "Dev1/ao6", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "555 AO-TriggerInputLine": "/Dev1/PFI0", "PRIME-ActualInterval-ms": "84.6200", "LudlController-Port": "COM9", "445 Shutter-Demo": "No", "PRIME-Trigger-Expose Out-Mux": "1", "SliceIndex": 10, "PRIME-CCDTemperatureSetPoint": "-15.0000", "405 Shutter-MinVolts": "0.0000", "PRIME-ExposeOutMode": "First Row", "AxisPositions": null, "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Volts": "0.0000", "528-Name": "DA Shutter", "555-State": "0", "XLIGHT Dichroic-Label": "Di03-R635-t1", "445 AO-Sequenceable": "1", "405-Name": "DA Shutter", "405 AO-IOChannel": "Dev1/ao0", "Core-Camera": "PRIME", "Focus-Name": "Focus", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "405 Shutter-IOChannel": "Dev1/ao8", "640 AO-Demo": "No", "640 Shutter-SupportsTriggering": "0", "445 AO-Demo": "No", "555-DA Device": "555 Shutter", "470 Shutter-Description": "NI DAC", "528 AO-Volts": "0.0000", "COM1-DataBits": "8", "Vincent-D3-Address": "x", "520 AO-SupportsTriggering": "0", "405-Description": "DA device that is used as a shutter", "640 Shutter-MinVolts": "0.0000", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "405 AO-Name": "AnalogIO", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "640 AO-TriggerInputLine": "/Dev1/PFI0", "405 AO-TriggerInputLine": "/Dev1/PFI0", "640-Description": "DA device that is used as a shutter", "445 Shutter-TriggerSampleRate": "100000", "470 Shutter-TriggerSequenceLength": "1000", "COM1-StopBits": "1", "520 Shutter-SupportsTriggering": "0", "405-State": "0", "Core-Initialize": "1", "XLIGHT Emission Wheel-State": "3", "520 AO-Description": "NI DAC", "528 AO-TriggerInputLine": "/Dev1/PFI0", "445 Shutter-MaxVolts": "5.0000", "COM9-Handshaking": "Off", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "XLIGHT Spin motor-Label": "On (spinning)", "PRIME-PP 5 ENABLED": "No", "UUID": "774608ac-bc2a-4a3c-bba9-e64a8d31fd86", "PRIME-TriggerMode": "Internal Trigger", "COM1-Handshaking": "Off", "PRIME-Timing-PostTriggerDelayNs": "49344000.0000", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "Focus-Position": "16732.9000", "528 Shutter-Block voltage": "false", "Channel": "P-DIC", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "555 Shutter-Demo": "No", "405 Shutter-Demo": "No", "528 AO-Name": "AnalogIO", "520-State": "0", "Core-Galvo": "", "405 Shutter-TriggerSampleRate": "100000", "Focus-FocusStepSize_(nm)": "100", "PRIME-PixelType": "16bit", "COM4-DataBits": "8", "470 AO-IOChannel": "Dev1/ao2", "520 AO-MinVolts": "0.0000", "COM9-Parity": "None", "PRIME-CircularBufferEnabled": "ON", "PRIME-PP 6 Reset": "No", "640 AO-Description": "NI DAC", "Source": "PRIME", "445 AO-MinVolts": "0.0000", "PIEZO-Name": "Stage", "445 AO-Description": "NI DAC", "PRIME-CircularBufferFrameCount": "46", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "520 AO-Volts": "0.0000", "528 Shutter-TriggerSampleRate": "100000", "XPositionUm": 23269.100000000002, "OpticalPath-State": "4", "PRIME-PP 3 THRESHOLD": "80", "PRIME-PVCAM Version": "3.7.1", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Gain": "1-HDR", "640 Shutter-TriggerSequenceLength": "1000", "528 AO-SupportsTriggering": "0", "445 AO-TriggerInputLine": "/Dev1/PFI0", "OpticalPath-Name": "OpticalPath", "PRIME-PP 4 ENABLED": "Yes", "PRIME-PVCAM Adapter Version": "1.3.29", "COM3-Name": "COM3", "405 AO-MaxVolts": "5.0000", "520 Shutter-Block voltage": "false", "PRIME-Timing-ClearingTimeNs": "49344000.0000", "Core-Focus": "PIEZO", "528 AO-Block voltage": "false", "TE2000-Description": "Nikon TE2000 hub", "528 AO-MinVolts": "0.0000", "COM4-Parity": "None", "Summary": { "Slices": 11, "ChContrastMin": [ 0, 0, 0, 0, 0, 0 ], "PixelAspect": 1, "SlicesFirst": true, "Interval_ms": 0, "Time": "2019-12-17 03:59:33 -0500", "Prefix": "seq_7", "InitialPositionList": [ { "Label": "Pos0", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [149.91], "Focus": [16657.7], "XYStage": [ 71629.7, 19152.9 ] }, "GridColumnIndex": 0 }, { "Label": "Pos1", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [190.82], "Focus": [16485.4], "XYStage": [ 65702.3, 19396.600000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos2", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [194.69], "Focus": [16446.8], "XYStage": [ 59880.5, 19007.8 ] }, "GridColumnIndex": 0 }, { "Label": "Pos3", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [145.04], "Focus": [16446.6], "XYStage": [ 53616.9, 18818.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos4", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [169.57500000000002], "Focus": [16446.5], "XYStage": [ 47662.8, 18817.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos5", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [131.805], "Focus": [16446.4], "XYStage": [ 41918.8, 18298.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos6", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [113.145], "Focus": [16446.5], "XYStage": [ 36129.9, 18895.8 ] }, "GridColumnIndex": 0 }, { "Label": "Pos7", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [141.005], "Focus": [16446.6], "XYStage": [ 30299.100000000002, 18895.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos8", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [140.97], "Focus": [16446.5], "XYStage": [ 23952.100000000002, 19156 ] }, "GridColumnIndex": 0 }, { "Label": "Pos9", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [184.57500000000002], "Focus": [16677.9], "XYStage": [ 72866.8, -14809.300000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos10", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [167.95000000000002], "Focus": [16557.9], "XYStage": [ 66515.6, -14371.300000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos11", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [168], "Focus": [16618.9], "XYStage": [ 59724.3, -13964.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos12", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [203.205], "Focus": [16779.6], "XYStage": [ 53862.3, -13494.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos13", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [203.20000000000002], "Focus": [16749], "XYStage": [ 48108.3, -13805.400000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos14", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [203.19], "Focus": [16675.9], "XYStage": [ 41645, -13804.900000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos15", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [203.21], "Focus": [16675.7], "XYStage": [ 35352.6, -13805.6 ] }, "GridColumnIndex": 0 }, { "Label": "Pos16", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [203.225], "Focus": [16675.8], "XYStage": [ 29298, -13447.400000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos17", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [203.215], "Focus": [16675.8], "XYStage": [ 23269.100000000002, -13012.300000000001 ] }, "GridColumnIndex": 0 } ], "z-step_um": 10, "GridRow": 0, "BitDepth": 16, "Depth": 2, "Channels": 6, "Source": "Micro-Manager", "ChColors": [ -16711681, -256, -65281, -1, -1, -16776961 ], "MetadataVersion": 10, "KeepShutterOpenChannels": true, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536, 65536 ], "CustomIntervals_ms": [], "Height": 1200, "MicroManagerVersion": "1.4.23 20180219", "GridColumn": 0, "UUID": "3ad8801b-5699-40d6-813b-4ad840863e38", "PixelType": "GRAY16", "Width": 1200, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\Yan\\20191216-YC113-S11S12-miseq", "Frames": 1, "Comment": "", "UserName": "Nikon", "PixelSize_um": 1.1, "ROI": [ 0, 0, 1200, 1200 ], "Date": "2019-12-17", "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC", "P-DAPI-high" ], "KeepShutterOpenSlices": true, "IJType": 1, "ComputerName": "ZadorConfocal2", "Positions": 18, "PositionIndex": 17, "CameraTimeout": 20000, "TimeFirst": false }, "640 AO-Name": "AnalogIO", "Vincent-D3-Command": "Close" }, "FrameKey-0-5-0": { "COM4-StopBits": "1", "528 AO-MaxVolts": "5.0000", "XLIGHT Excitation Wheel-State": "1", "470 Shutter-Name": "AnalogIO", "Core-Shutter": "405", "555 AO-Demo": "No", "XLIGHT Dichroic-State": "2", "XYStage-StepSize-Y": "0.1000", "ZPositionUm": 153.215, "XYStage-StepSize-X": "0.1000", "PRIME-FirmwareVersion": "20.28", "PRIME-ClearCycles": "2", "520-Name": "DA Shutter", "405 Shutter-Name": "AnalogIO", "PIEZO-StepSize": "0.0050", "PRIME-ClearMode": "Pre-Exposure", "470 AO-MinVolts": "0.0000", "405 AO-SupportsTriggering": "1", "CameraChannelIndex": 0, "XYStage-StartSpeed": "500.0000", "COM9-StopBits": "2", "405 AO-Description": "NI DAC", "COM4-DelayBetweenCharsMs": "0.0000", "445 AO-Block voltage": "false", "520 AO-TriggerInputLine": "/Dev1/PFI0", "555 AO-Description": "NI DAC", "520 AO-TriggerSampleRate": "100000", "COM4-Verbose": "1", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM4-Name": "COM4", "405 Shutter-Volts": "5.0000", "Vincent-D3-Port": "COM1", "PRIME-PP 4 MIN ADU AFFECTED": "200", "470 Shutter-MinVolts": "0.0000", "PRIME-PP 3 ENABLED": "Yes", "470 AO-Description": "NI DAC", "PRIME-Description": "PVCAM API device adapter", "520 Shutter-MaxVolts": "5.0000", "520 Shutter-Volts": "0.0000", "528 AO-Sequenceable": "1", "PRIME-Binning": "1x1", "555 Shutter-MaxVolts": "5.0000", "528 Shutter-TriggerSequenceLength": "1000", "405 Shutter-Block voltage": "false", "XLIGHT Spin motor-State": "1", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "Vincent-D3-Time to open (ms)": "35.0000", "Vincent-D3-Time to close (ms)": "35.0000", "445 AO-TriggerSampleRate": "100000", "640 Shutter-IOChannel": "Dev1/ao14", "555 AO-Sequenceable": "1", "520 AO-Name": "AnalogIO", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "470 AO-Block voltage": "false", "COM1-Verbose": "1", "COM9-AnswerTimeout": "10000.0000", "528 Shutter-Name": "AnalogIO", "PRIME-Trigger Timeout (secs)": "10", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "NextFrame": 0, "470 AO-TriggerSequenceLength": "1000", "PRIME-TransposeXY": "0", "445 AO-TriggerSequenceLength": "1000", "COM1-Name": "COM1", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "COM9-DataBits": "8", "COM1-AnswerTimeout": "500.0000", "Camera": "", "TE2000-Version": "V2.50-V2.50-V2.10", "528 Shutter-Volts": "0.0000", "XLIGHT Hub-Name": "XLIGHT Hub", "PIEZO-Description": "Ludl stage driver adapter", "PositionName": "Pos17", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSampleRate": "100000", "PRIME-FanSpeedSetpoint": "High", "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "405 AO-TriggerSampleRate": "100000", "470 Shutter-Block voltage": "false", "470-State": "0", "COM3-Parity": "None", "405 AO-Volts": "4.5000", "PRIME-TransposeMirrorY": "0", "PRIME-ChipName": "GS144BSI", "470 AO-Volts": "0.0000", "PRIME-TransposeMirrorX": "0", "445 Shutter-SupportsTriggering": "0", "470 Shutter-SupportsTriggering": "0", "XLIGHT Hub-Description": "XLIGHT hub", "PRIME-PreampOffLimit": "10000", "470 AO-Sequenceable": "1", "528 Shutter-MaxVolts": "5.0000", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "Core-XYStage": "XYStage", "470 AO-TriggerSampleRate": "100000", "PRIME-Timing-PreTriggerDelayNs": "24672000.0000", "528-DA Device": "528 Shutter", "Focus-Description": "Nikon TE2000 focus adapter", "FilterBlock-State": "5", "520 AO-Demo": "No", "COM3-DelayBetweenCharsMs": "0.0000", "470 Shutter-Volts": "0.0000", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "PRIME-PP 2 THRESHOLD": "125", "PRIME-PP 4 THRESHOLD": "75", "XYStage-Acceleration": "4.0000", "470 Shutter-Demo": "No", "PRIME-Name": "PMPCIECam00", "640-DA Device": "640 Shutter", "405 AO-Block voltage": "false", "ElapsedTime-ms": 748121, "PRIME-Offset": "100", "445 AO-IOChannel": "Dev1/ao1", "640 AO-MaxVolts": "5.0000", "PositionIndex": 17, "PRIME-PP 1 ENABLED": "Yes", "COM1-BaudRate": "9600", "640-State": "0", "COM3-Verbose": "1", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "LudlController-TransmissionDelay": "0", "PRIME-FullWellCapacity": "62000", "PRIME-SMARTStreamingEnabled": "No", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "640 Shutter-Description": "NI DAC", "528-Description": "DA device that is used as a shutter", "COM4-Description": "Serial port driver (boost:asio)", "405 Shutter-Description": "NI DAC", "TE2000-Name": "TE2000", "528 Shutter-Description": "NI DAC", "COM9-Description": "Serial port driver (boost:asio)", "LudlController-Name": "LudlController", "445 Shutter-TriggerSequenceLength": "1000", "PRIME-SerialNumber": "A18B203008", "445 AO-Volts": "0.0000", "PRIME-X-dimension": "1200", "PRIME-ShutterCloseDelay": "0", "XLIGHT Disk slider-State": "2", "Position": "Default", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 AO-IOChannel": "Dev1/ao5", "520 AO-IOChannel": "Dev1/ao4", "528 AO-TriggerSequenceLength": "1000", "405 Shutter-MaxVolts": "5.0000", "470-DA Device": "470 Shutter", "PRIME-FTCapable": "No", "Core-ChannelGroup": "Channels", "555 AO-SupportsTriggering": "0", "PRIME-Y-dimension": "1200", "Frame": 0, "555 Shutter-Sequenceable": "1", "PRIME-Timing-ExposureTimeNs": "499998000.0000", "Core-ImageProcessor": "", "528-State": "0", "520 AO-Block voltage": "false", "XLIGHT Excitation Wheel-Label": "QUAD", "640 Shutter-Demo": "No", "COM3-StopBits": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "520 Shutter-IOChannel": "Dev1/ao11", "Width": 1200, "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-SupportsTriggering": "0", "640 AO-TriggerSampleRate": "100000", "520-Description": "DA device that is used as a shutter", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "405 Shutter-Sequenceable": "1", "405 Shutter-SupportsTriggering": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-TriggerSampleRate": "100000", "640 Shutter-MaxVolts": "5.0000", "555 AO-MaxVolts": "5.0000", "520 AO-TriggerSequenceLength": "1000", "COM3-BaudRate": "9600", "COM9-BaudRate": "9600", "Vincent-D3-Channel #": "Ch. #1", "PRIME-PMode": "Normal", "OpticalPath-Label": "Left(100)", "BitDepth": 16, "555 AO-TriggerSequenceLength": "1000", "445 Shutter-Description": "NI DAC", "405 AO-Sequenceable": "1", "PRIME-AcquisitionMethod": "Callbacks", "555 AO-Name": "AnalogIO", "PRIME-ShutterMode": "Pre-Exposure", "COM3-Handshaking": "Off", "445 AO-SupportsTriggering": "0", "ROI": "0-0-1200-1200", "COM9-Verbose": "1", "PRIME-Color": "OFF", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "PRIME-ShutterOpenDelay": "0", "COM1-Parity": "None", "Slice": 0, "SlicePosition": -50, "COM9-DelayBetweenCharsMs": "0.0000", "555-Name": "DA Shutter", "PIEZO-LudlSingleAxisName": "B", "XLIGHT Emission Wheel-Label": "QUAD", "640 Shutter-Name": "AnalogIO", "640 AO-Sequenceable": "1", "520 Shutter-Demo": "No", "528 Shutter-Demo": "No", "470-Name": "DA Shutter", "520-DA Device": "520 Shutter", "XYStage-Description": "Ludl XY stage driver adapter", "445 Shutter-MinVolts": "0.0000", "528 AO-TriggerSampleRate": "100000", "405 AO-MinVolts": "0.0000", "405 Shutter-TriggerSequenceLength": "1000", "Height": 1200, "555 AO-Block voltage": "false", "640 Shutter-Volts": "0.0000", "PixelType": "GRAY16", "445 Shutter-Block voltage": "false", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "520 Shutter-TriggerSequenceLength": "1000", "COM9-Name": "COM9", "Core-SLM": "", "555 AO-Volts": "0.0000", "555 Shutter-Block voltage": "false", "XYStage-Speed": "1800.0000", "520 AO-MaxVolts": "5.0000", "528 Shutter-SupportsTriggering": "0", "COM1-Description": "Serial port driver (boost:asio)", "PRIME-MetadataEnabled": "No", "520 Shutter-MinVolts": "0.0000", "470 Shutter-Sequenceable": "1", "PRIME-PreampDelay": "0", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-MinVolts": "0.0000", "ChannelIndex": 5, "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Core-AutoShutter": "1", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "Exposure-ms": 300, "PRIME-PP 2 ENABLED": "Yes", "640 Shutter-TriggerSampleRate": "100000", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "470 Shutter-IOChannel": "Dev1/ao10", "405 AO-Demo": "No", "PixelSizeUm": 1.1, "470 AO-Demo": "No", "470 Shutter-MaxVolts": "5.0000", "Vincent-D3-Name": "Vincent-D3", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FileName": "img_000000000_P-DAPI-high_000.tif", "LudlController-Reset": "Operate", "PRIME-Exposure": "300.00", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "445-Name": "DA Shutter", "555 Shutter-Name": "AnalogIO", "520 Shutter-TriggerSampleRate": "100000", "445 Shutter-IOChannel": "Dev1/ao9", "PRIME-TransposeCorrection": "0", "XYStage-StepSize": "0.1000", "445-Description": "DA device that is used as a shutter", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 AO-IOChannel": "Dev1/ao7", "520 Shutter-Name": "AnalogIO", "COM3-DataBits": "8", "640 AO-Volts": "0.0000", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "555 Shutter-IOChannel": "Dev1/ao13", "WaitInterval": null, "520 Shutter-Sequenceable": "1", "YPositionUm": -13012.300000000001, "445-State": "0", "445-DA Device": "445 Shutter", "PRIME-CircularBufferAutoSize": "ON", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "555 AO-TriggerSampleRate": "100000", "445 Shutter-Volts": "0.0000", "XLIGHT Hub-Port": "COM3", "Core-AutoFocus": "", "COM4-Handshaking": "Off", "528 Shutter-MinVolts": "0.0000", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "445 Shutter-Name": "AnalogIO", "528 Shutter-Sequenceable": "1", "XYStage-TransposeMirrorX": "0", "XYStage-TransposeMirrorY": "0", "PRIME-ReadoutRate": "100MHz 16bit", "XYStage-Name": "Stage", "640 AO-SupportsTriggering": "0", "640-Name": "DA Shutter", "640 Shutter-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "640 AO-Block voltage": "false", "445 Shutter-Sequenceable": "1", "470 AO-MaxVolts": "5.0000", "COM4-BaudRate": "9600", "470-Description": "DA device that is used as a shutter", "520 Shutter-Description": "NI DAC", "PRIME-CCDTemperature": "-14.9200", "Time": "2019-12-17 04:00:05 -0500", "640 AO-TriggerSequenceLength": "1000", "470 AO-Name": "AnalogIO", "XLIGHT Disk slider-Label": "Disk Pos 40um", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "Binning": "1x1", "TE2000-ModelType": "1", "COM3-AnswerTimeout": "500.0000", "555 Shutter-SupportsTriggering": "0", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "TE2000-Port": "COM4", "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Description": "NI DAC", "COM4-AnswerTimeout": "500.0000", "COM1-DelayBetweenCharsMs": "0.0000", "FilterBlock-Label": "Confocal", "445 AO-Name": "AnalogIO", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-Timing-ReadoutTimeNs": "24672000.0000", "528 AO-Demo": "No", "520 AO-Sequenceable": "1", "555-Description": "DA device that is used as a shutter", "405-DA Device": "405 Shutter", "405 AO-TriggerSequenceLength": "1000", "640 AO-MinVolts": "0.0000", "640 Shutter-Block voltage": "false", "FrameIndex": 0, "PRIME-Port": "CMOS", "555 AO-MinVolts": "0.0000", "COM3-Description": "Serial port driver (boost:asio)", "528 AO-Description": "NI DAC", "555 AO-IOChannel": "Dev1/ao6", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "555 AO-TriggerInputLine": "/Dev1/PFI0", "PRIME-ActualInterval-ms": "84.6200", "LudlController-Port": "COM9", "445 Shutter-Demo": "No", "PRIME-Trigger-Expose Out-Mux": "1", "SliceIndex": 0, "PRIME-CCDTemperatureSetPoint": "-15.0000", "405 Shutter-MinVolts": "0.0000", "PRIME-ExposeOutMode": "First Row", "AxisPositions": null, "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Volts": "0.0000", "528-Name": "DA Shutter", "555-State": "0", "XLIGHT Dichroic-Label": "QUAD", "445 AO-Sequenceable": "1", "405-Name": "DA Shutter", "405 AO-IOChannel": "Dev1/ao0", "Core-Camera": "PRIME", "Focus-Name": "Focus", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "405 Shutter-IOChannel": "Dev1/ao8", "640 AO-Demo": "No", "640 Shutter-SupportsTriggering": "0", "445 AO-Demo": "No", "555-DA Device": "555 Shutter", "470 Shutter-Description": "NI DAC", "528 AO-Volts": "0.0000", "COM1-DataBits": "8", "Vincent-D3-Address": "x", "520 AO-SupportsTriggering": "0", "405-Description": "DA device that is used as a shutter", "640 Shutter-MinVolts": "0.0000", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "405 AO-Name": "AnalogIO", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "640 AO-TriggerInputLine": "/Dev1/PFI0", "405 AO-TriggerInputLine": "/Dev1/PFI0", "640-Description": "DA device that is used as a shutter", "445 Shutter-TriggerSampleRate": "100000", "470 Shutter-TriggerSequenceLength": "1000", "COM1-StopBits": "1", "520 Shutter-SupportsTriggering": "0", "405-State": "1", "Core-Initialize": "1", "XLIGHT Emission Wheel-State": "3", "520 AO-Description": "NI DAC", "528 AO-TriggerInputLine": "/Dev1/PFI0", "445 Shutter-MaxVolts": "5.0000", "COM9-Handshaking": "Off", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "XLIGHT Spin motor-Label": "On (spinning)", "PRIME-PP 5 ENABLED": "No", "UUID": "fb1498d1-3b7e-4cf1-a2be-0685570c1f96", "PRIME-TriggerMode": "Internal Trigger", "COM1-Handshaking": "Off", "PRIME-Timing-PostTriggerDelayNs": "49344000.0000", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "Focus-Position": "16732.9000", "528 Shutter-Block voltage": "false", "Channel": "P-DAPI-high", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "555 Shutter-Demo": "No", "405 Shutter-Demo": "No", "528 AO-Name": "AnalogIO", "520-State": "0", "Core-Galvo": "", "405 Shutter-TriggerSampleRate": "100000", "Focus-FocusStepSize_(nm)": "100", "PRIME-PixelType": "16bit", "COM4-DataBits": "8", "470 AO-IOChannel": "Dev1/ao2", "520 AO-MinVolts": "0.0000", "COM9-Parity": "None", "PRIME-CircularBufferEnabled": "ON", "PRIME-PP 6 Reset": "No", "640 AO-Description": "NI DAC", "Source": "PRIME", "445 AO-MinVolts": "0.0000", "PIEZO-Name": "Stage", "445 AO-Description": "NI DAC", "PRIME-CircularBufferFrameCount": "46", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "520 AO-Volts": "0.0000", "528 Shutter-TriggerSampleRate": "100000", "XPositionUm": 23269.100000000002, "OpticalPath-State": "4", "PRIME-PP 3 THRESHOLD": "80", "PRIME-PVCAM Version": "3.7.1", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Gain": "1-HDR", "640 Shutter-TriggerSequenceLength": "1000", "528 AO-SupportsTriggering": "0", "445 AO-TriggerInputLine": "/Dev1/PFI0", "OpticalPath-Name": "OpticalPath", "PRIME-PP 4 ENABLED": "Yes", "PRIME-PVCAM Adapter Version": "1.3.29", "COM3-Name": "COM3", "405 AO-MaxVolts": "5.0000", "520 Shutter-Block voltage": "false", "PRIME-Timing-ClearingTimeNs": "49344000.0000", "Core-Focus": "PIEZO", "528 AO-Block voltage": "false", "TE2000-Description": "Nikon TE2000 hub", "528 AO-MinVolts": "0.0000", "COM4-Parity": "None", "Summary": { "Slices": 11, "ChContrastMin": [ 0, 0, 0, 0, 0, 0 ], "PixelAspect": 1, "SlicesFirst": true, "Interval_ms": 0, "Time": "2019-12-17 03:59:33 -0500", "Prefix": "seq_7", "InitialPositionList": [ { "Label": "Pos0", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [149.91], "Focus": [16657.7], "XYStage": [ 71629.7, 19152.9 ] }, "GridColumnIndex": 0 }, { "Label": "Pos1", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [190.82], "Focus": [16485.4], "XYStage": [ 65702.3, 19396.600000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos2", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [194.69], "Focus": [16446.8], "XYStage": [ 59880.5, 19007.8 ] }, "GridColumnIndex": 0 }, { "Label": "Pos3", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [145.04], "Focus": [16446.6], "XYStage": [ 53616.9, 18818.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos4", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [169.57500000000002], "Focus": [16446.5], "XYStage": [ 47662.8, 18817.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos5", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [131.805], "Focus": [16446.4], "XYStage": [ 41918.8, 18298.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos6", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [113.145], "Focus": [16446.5], "XYStage": [ 36129.9, 18895.8 ] }, "GridColumnIndex": 0 }, { "Label": "Pos7", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [141.005], "Focus": [16446.6], "XYStage": [ 30299.100000000002, 18895.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos8", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [140.97], "Focus": [16446.5], "XYStage": [ 23952.100000000002, 19156 ] }, "GridColumnIndex": 0 }, { "Label": "Pos9", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [184.57500000000002], "Focus": [16677.9], "XYStage": [ 72866.8, -14809.300000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos10", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [167.95000000000002], "Focus": [16557.9], "XYStage": [ 66515.6, -14371.300000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos11", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [168], "Focus": [16618.9], "XYStage": [ 59724.3, -13964.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos12", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [203.205], "Focus": [16779.6], "XYStage": [ 53862.3, -13494.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos13", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [203.20000000000002], "Focus": [16749], "XYStage": [ 48108.3, -13805.400000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos14", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [203.19], "Focus": [16675.9], "XYStage": [ 41645, -13804.900000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos15", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [203.21], "Focus": [16675.7], "XYStage": [ 35352.6, -13805.6 ] }, "GridColumnIndex": 0 }, { "Label": "Pos16", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [203.225], "Focus": [16675.8], "XYStage": [ 29298, -13447.400000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos17", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [203.215], "Focus": [16675.8], "XYStage": [ 23269.100000000002, -13012.300000000001 ] }, "GridColumnIndex": 0 } ], "z-step_um": 10, "GridRow": 0, "BitDepth": 16, "Depth": 2, "Channels": 6, "Source": "Micro-Manager", "ChColors": [ -16711681, -256, -65281, -1, -1, -16776961 ], "MetadataVersion": 10, "KeepShutterOpenChannels": true, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536, 65536 ], "CustomIntervals_ms": [], "Height": 1200, "MicroManagerVersion": "1.4.23 20180219", "GridColumn": 0, "UUID": "3ad8801b-5699-40d6-813b-4ad840863e38", "PixelType": "GRAY16", "Width": 1200, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\Yan\\20191216-YC113-S11S12-miseq", "Frames": 1, "Comment": "", "UserName": "Nikon", "PixelSize_um": 1.1, "ROI": [ 0, 0, 1200, 1200 ], "Date": "2019-12-17", "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC", "P-DAPI-high" ], "KeepShutterOpenSlices": true, "IJType": 1, "ComputerName": "ZadorConfocal2", "Positions": 18, "PositionIndex": 17, "CameraTimeout": 20000, "TimeFirst": false }, "640 AO-Name": "AnalogIO", "Vincent-D3-Command": "Close" }, "FrameKey-0-5-1": { "COM4-StopBits": "1", "528 AO-MaxVolts": "5.0000", "XLIGHT Excitation Wheel-State": "1", "470 Shutter-Name": "AnalogIO", "Core-Shutter": "405", "555 AO-Demo": "No", "XLIGHT Dichroic-State": "2", "XYStage-StepSize-Y": "0.1000", "ZPositionUm": 163.215, "XYStage-StepSize-X": "0.1000", "PRIME-FirmwareVersion": "20.28", "PRIME-ClearCycles": "2", "520-Name": "DA Shutter", "405 Shutter-Name": "AnalogIO", "PIEZO-StepSize": "0.0050", "PRIME-ClearMode": "Pre-Exposure", "470 AO-MinVolts": "0.0000", "405 AO-SupportsTriggering": "1", "CameraChannelIndex": 0, "XYStage-StartSpeed": "500.0000", "COM9-StopBits": "2", "405 AO-Description": "NI DAC", "COM4-DelayBetweenCharsMs": "0.0000", "445 AO-Block voltage": "false", "520 AO-TriggerInputLine": "/Dev1/PFI0", "555 AO-Description": "NI DAC", "520 AO-TriggerSampleRate": "100000", "COM4-Verbose": "1", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM4-Name": "COM4", "405 Shutter-Volts": "5.0000", "Vincent-D3-Port": "COM1", "PRIME-PP 4 MIN ADU AFFECTED": "200", "470 Shutter-MinVolts": "0.0000", "PRIME-PP 3 ENABLED": "Yes", "470 AO-Description": "NI DAC", "PRIME-Description": "PVCAM API device adapter", "520 Shutter-MaxVolts": "5.0000", "520 Shutter-Volts": "0.0000", "528 AO-Sequenceable": "1", "PRIME-Binning": "1x1", "555 Shutter-MaxVolts": "5.0000", "528 Shutter-TriggerSequenceLength": "1000", "405 Shutter-Block voltage": "false", "XLIGHT Spin motor-State": "1", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "Vincent-D3-Time to open (ms)": "35.0000", "Vincent-D3-Time to close (ms)": "35.0000", "445 AO-TriggerSampleRate": "100000", "640 Shutter-IOChannel": "Dev1/ao14", "555 AO-Sequenceable": "1", "520 AO-Name": "AnalogIO", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "470 AO-Block voltage": "false", "COM1-Verbose": "1", "COM9-AnswerTimeout": "10000.0000", "528 Shutter-Name": "AnalogIO", "PRIME-Trigger Timeout (secs)": "10", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "NextFrame": 0, "470 AO-TriggerSequenceLength": "1000", "PRIME-TransposeXY": "0", "445 AO-TriggerSequenceLength": "1000", "COM1-Name": "COM1", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "COM9-DataBits": "8", "COM1-AnswerTimeout": "500.0000", "Camera": "", "TE2000-Version": "V2.50-V2.50-V2.10", "528 Shutter-Volts": "0.0000", "XLIGHT Hub-Name": "XLIGHT Hub", "PIEZO-Description": "Ludl stage driver adapter", "PositionName": "Pos17", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSampleRate": "100000", "PRIME-FanSpeedSetpoint": "High", "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "405 AO-TriggerSampleRate": "100000", "470 Shutter-Block voltage": "false", "470-State": "0", "COM3-Parity": "None", "405 AO-Volts": "4.5000", "PRIME-TransposeMirrorY": "0", "PRIME-ChipName": "GS144BSI", "470 AO-Volts": "0.0000", "PRIME-TransposeMirrorX": "0", "445 Shutter-SupportsTriggering": "0", "470 Shutter-SupportsTriggering": "0", "XLIGHT Hub-Description": "XLIGHT hub", "PRIME-PreampOffLimit": "10000", "470 AO-Sequenceable": "1", "528 Shutter-MaxVolts": "5.0000", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "Core-XYStage": "XYStage", "470 AO-TriggerSampleRate": "100000", "PRIME-Timing-PreTriggerDelayNs": "24672000.0000", "528-DA Device": "528 Shutter", "Focus-Description": "Nikon TE2000 focus adapter", "FilterBlock-State": "5", "520 AO-Demo": "No", "COM3-DelayBetweenCharsMs": "0.0000", "470 Shutter-Volts": "0.0000", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "PRIME-PP 2 THRESHOLD": "125", "PRIME-PP 4 THRESHOLD": "75", "XYStage-Acceleration": "4.0000", "470 Shutter-Demo": "No", "PRIME-Name": "PMPCIECam00", "640-DA Device": "640 Shutter", "405 AO-Block voltage": "false", "ElapsedTime-ms": 748659, "PRIME-Offset": "100", "445 AO-IOChannel": "Dev1/ao1", "640 AO-MaxVolts": "5.0000", "PositionIndex": 17, "PRIME-PP 1 ENABLED": "Yes", "COM1-BaudRate": "9600", "640-State": "0", "COM3-Verbose": "1", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "LudlController-TransmissionDelay": "0", "PRIME-FullWellCapacity": "62000", "PRIME-SMARTStreamingEnabled": "No", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "640 Shutter-Description": "NI DAC", "528-Description": "DA device that is used as a shutter", "COM4-Description": "Serial port driver (boost:asio)", "405 Shutter-Description": "NI DAC", "TE2000-Name": "TE2000", "528 Shutter-Description": "NI DAC", "COM9-Description": "Serial port driver (boost:asio)", "LudlController-Name": "LudlController", "445 Shutter-TriggerSequenceLength": "1000", "PRIME-SerialNumber": "A18B203008", "445 AO-Volts": "0.0000", "PRIME-X-dimension": "1200", "PRIME-ShutterCloseDelay": "0", "XLIGHT Disk slider-State": "2", "Position": "Default", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 AO-IOChannel": "Dev1/ao5", "520 AO-IOChannel": "Dev1/ao4", "528 AO-TriggerSequenceLength": "1000", "405 Shutter-MaxVolts": "5.0000", "470-DA Device": "470 Shutter", "PRIME-FTCapable": "No", "Core-ChannelGroup": "Channels", "555 AO-SupportsTriggering": "0", "PRIME-Y-dimension": "1200", "Frame": 0, "555 Shutter-Sequenceable": "1", "PRIME-Timing-ExposureTimeNs": "499998000.0000", "Core-ImageProcessor": "", "528-State": "0", "520 AO-Block voltage": "false", "XLIGHT Excitation Wheel-Label": "QUAD", "640 Shutter-Demo": "No", "COM3-StopBits": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "520 Shutter-IOChannel": "Dev1/ao11", "Width": 1200, "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-SupportsTriggering": "0", "640 AO-TriggerSampleRate": "100000", "520-Description": "DA device that is used as a shutter", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "405 Shutter-Sequenceable": "1", "405 Shutter-SupportsTriggering": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-TriggerSampleRate": "100000", "640 Shutter-MaxVolts": "5.0000", "555 AO-MaxVolts": "5.0000", "520 AO-TriggerSequenceLength": "1000", "COM3-BaudRate": "9600", "COM9-BaudRate": "9600", "Vincent-D3-Channel #": "Ch. #1", "PRIME-PMode": "Normal", "OpticalPath-Label": "Left(100)", "BitDepth": 16, "555 AO-TriggerSequenceLength": "1000", "445 Shutter-Description": "NI DAC", "405 AO-Sequenceable": "1", "PRIME-AcquisitionMethod": "Callbacks", "555 AO-Name": "AnalogIO", "PRIME-ShutterMode": "Pre-Exposure", "COM3-Handshaking": "Off", "445 AO-SupportsTriggering": "0", "ROI": "0-0-1200-1200", "COM9-Verbose": "1", "PRIME-Color": "OFF", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "PRIME-ShutterOpenDelay": "0", "COM1-Parity": "None", "Slice": 1, "SlicePosition": -40, "COM9-DelayBetweenCharsMs": "0.0000", "555-Name": "DA Shutter", "PIEZO-LudlSingleAxisName": "B", "XLIGHT Emission Wheel-Label": "QUAD", "640 Shutter-Name": "AnalogIO", "640 AO-Sequenceable": "1", "520 Shutter-Demo": "No", "528 Shutter-Demo": "No", "470-Name": "DA Shutter", "520-DA Device": "520 Shutter", "XYStage-Description": "Ludl XY stage driver adapter", "445 Shutter-MinVolts": "0.0000", "528 AO-TriggerSampleRate": "100000", "405 AO-MinVolts": "0.0000", "405 Shutter-TriggerSequenceLength": "1000", "Height": 1200, "555 AO-Block voltage": "false", "640 Shutter-Volts": "0.0000", "PixelType": "GRAY16", "445 Shutter-Block voltage": "false", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "520 Shutter-TriggerSequenceLength": "1000", "COM9-Name": "COM9", "Core-SLM": "", "555 AO-Volts": "0.0000", "555 Shutter-Block voltage": "false", "XYStage-Speed": "1800.0000", "520 AO-MaxVolts": "5.0000", "528 Shutter-SupportsTriggering": "0", "COM1-Description": "Serial port driver (boost:asio)", "PRIME-MetadataEnabled": "No", "520 Shutter-MinVolts": "0.0000", "470 Shutter-Sequenceable": "1", "PRIME-PreampDelay": "0", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-MinVolts": "0.0000", "ChannelIndex": 5, "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Core-AutoShutter": "1", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "Exposure-ms": 300, "PRIME-PP 2 ENABLED": "Yes", "640 Shutter-TriggerSampleRate": "100000", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "470 Shutter-IOChannel": "Dev1/ao10", "405 AO-Demo": "No", "PixelSizeUm": 1.1, "470 AO-Demo": "No", "470 Shutter-MaxVolts": "5.0000", "Vincent-D3-Name": "Vincent-D3", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FileName": "img_000000000_P-DAPI-high_001.tif", "LudlController-Reset": "Operate", "PRIME-Exposure": "300.00", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "445-Name": "DA Shutter", "555 Shutter-Name": "AnalogIO", "520 Shutter-TriggerSampleRate": "100000", "445 Shutter-IOChannel": "Dev1/ao9", "PRIME-TransposeCorrection": "0", "XYStage-StepSize": "0.1000", "445-Description": "DA device that is used as a shutter", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 AO-IOChannel": "Dev1/ao7", "520 Shutter-Name": "AnalogIO", "COM3-DataBits": "8", "640 AO-Volts": "0.0000", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "555 Shutter-IOChannel": "Dev1/ao13", "WaitInterval": null, "520 Shutter-Sequenceable": "1", "YPositionUm": -13012.300000000001, "445-State": "0", "445-DA Device": "445 Shutter", "PRIME-CircularBufferAutoSize": "ON", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "555 AO-TriggerSampleRate": "100000", "445 Shutter-Volts": "0.0000", "XLIGHT Hub-Port": "COM3", "Core-AutoFocus": "", "COM4-Handshaking": "Off", "528 Shutter-MinVolts": "0.0000", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "445 Shutter-Name": "AnalogIO", "528 Shutter-Sequenceable": "1", "XYStage-TransposeMirrorX": "0", "XYStage-TransposeMirrorY": "0", "PRIME-ReadoutRate": "100MHz 16bit", "XYStage-Name": "Stage", "640 AO-SupportsTriggering": "0", "640-Name": "DA Shutter", "640 Shutter-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "640 AO-Block voltage": "false", "445 Shutter-Sequenceable": "1", "470 AO-MaxVolts": "5.0000", "COM4-BaudRate": "9600", "470-Description": "DA device that is used as a shutter", "520 Shutter-Description": "NI DAC", "PRIME-CCDTemperature": "-14.9200", "Time": "2019-12-17 04:00:05 -0500", "640 AO-TriggerSequenceLength": "1000", "470 AO-Name": "AnalogIO", "XLIGHT Disk slider-Label": "Disk Pos 40um", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "Binning": "1x1", "TE2000-ModelType": "1", "COM3-AnswerTimeout": "500.0000", "555 Shutter-SupportsTriggering": "0", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "TE2000-Port": "COM4", "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Description": "NI DAC", "COM4-AnswerTimeout": "500.0000", "COM1-DelayBetweenCharsMs": "0.0000", "FilterBlock-Label": "Confocal", "445 AO-Name": "AnalogIO", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-Timing-ReadoutTimeNs": "24672000.0000", "528 AO-Demo": "No", "520 AO-Sequenceable": "1", "555-Description": "DA device that is used as a shutter", "405-DA Device": "405 Shutter", "405 AO-TriggerSequenceLength": "1000", "640 AO-MinVolts": "0.0000", "640 Shutter-Block voltage": "false", "FrameIndex": 0, "PRIME-Port": "CMOS", "555 AO-MinVolts": "0.0000", "COM3-Description": "Serial port driver (boost:asio)", "528 AO-Description": "NI DAC", "555 AO-IOChannel": "Dev1/ao6", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "555 AO-TriggerInputLine": "/Dev1/PFI0", "PRIME-ActualInterval-ms": "84.6200", "LudlController-Port": "COM9", "445 Shutter-Demo": "No", "PRIME-Trigger-Expose Out-Mux": "1", "SliceIndex": 1, "PRIME-CCDTemperatureSetPoint": "-15.0000", "405 Shutter-MinVolts": "0.0000", "PRIME-ExposeOutMode": "First Row", "AxisPositions": null, "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Volts": "0.0000", "528-Name": "DA Shutter", "555-State": "0", "XLIGHT Dichroic-Label": "QUAD", "445 AO-Sequenceable": "1", "405-Name": "DA Shutter", "405 AO-IOChannel": "Dev1/ao0", "Core-Camera": "PRIME", "Focus-Name": "Focus", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "405 Shutter-IOChannel": "Dev1/ao8", "640 AO-Demo": "No", "640 Shutter-SupportsTriggering": "0", "445 AO-Demo": "No", "555-DA Device": "555 Shutter", "470 Shutter-Description": "NI DAC", "528 AO-Volts": "0.0000", "COM1-DataBits": "8", "Vincent-D3-Address": "x", "520 AO-SupportsTriggering": "0", "405-Description": "DA device that is used as a shutter", "640 Shutter-MinVolts": "0.0000", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "405 AO-Name": "AnalogIO", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "640 AO-TriggerInputLine": "/Dev1/PFI0", "405 AO-TriggerInputLine": "/Dev1/PFI0", "640-Description": "DA device that is used as a shutter", "445 Shutter-TriggerSampleRate": "100000", "470 Shutter-TriggerSequenceLength": "1000", "COM1-StopBits": "1", "520 Shutter-SupportsTriggering": "0", "405-State": "1", "Core-Initialize": "1", "XLIGHT Emission Wheel-State": "3", "520 AO-Description": "NI DAC", "528 AO-TriggerInputLine": "/Dev1/PFI0", "445 Shutter-MaxVolts": "5.0000", "COM9-Handshaking": "Off", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "XLIGHT Spin motor-Label": "On (spinning)", "PRIME-PP 5 ENABLED": "No", "UUID": "9b5b5dfa-1129-442c-a1b6-5bae504f4f18", "PRIME-TriggerMode": "Internal Trigger", "COM1-Handshaking": "Off", "PRIME-Timing-PostTriggerDelayNs": "49344000.0000", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "Focus-Position": "16732.9000", "528 Shutter-Block voltage": "false", "Channel": "P-DAPI-high", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "555 Shutter-Demo": "No", "405 Shutter-Demo": "No", "528 AO-Name": "AnalogIO", "520-State": "0", "Core-Galvo": "", "405 Shutter-TriggerSampleRate": "100000", "Focus-FocusStepSize_(nm)": "100", "PRIME-PixelType": "16bit", "COM4-DataBits": "8", "470 AO-IOChannel": "Dev1/ao2", "520 AO-MinVolts": "0.0000", "COM9-Parity": "None", "PRIME-CircularBufferEnabled": "ON", "PRIME-PP 6 Reset": "No", "640 AO-Description": "NI DAC", "Source": "PRIME", "445 AO-MinVolts": "0.0000", "PIEZO-Name": "Stage", "445 AO-Description": "NI DAC", "PRIME-CircularBufferFrameCount": "46", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "520 AO-Volts": "0.0000", "528 Shutter-TriggerSampleRate": "100000", "XPositionUm": 23269.100000000002, "OpticalPath-State": "4", "PRIME-PP 3 THRESHOLD": "80", "PRIME-PVCAM Version": "3.7.1", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Gain": "1-HDR", "640 Shutter-TriggerSequenceLength": "1000", "528 AO-SupportsTriggering": "0", "445 AO-TriggerInputLine": "/Dev1/PFI0", "OpticalPath-Name": "OpticalPath", "PRIME-PP 4 ENABLED": "Yes", "PRIME-PVCAM Adapter Version": "1.3.29", "COM3-Name": "COM3", "405 AO-MaxVolts": "5.0000", "520 Shutter-Block voltage": "false", "PRIME-Timing-ClearingTimeNs": "49344000.0000", "Core-Focus": "PIEZO", "528 AO-Block voltage": "false", "TE2000-Description": "Nikon TE2000 hub", "528 AO-MinVolts": "0.0000", "COM4-Parity": "None", "Summary": { "Slices": 11, "ChContrastMin": [ 0, 0, 0, 0, 0, 0 ], "PixelAspect": 1, "SlicesFirst": true, "Interval_ms": 0, "Time": "2019-12-17 03:59:33 -0500", "Prefix": "seq_7", "InitialPositionList": [ { "Label": "Pos0", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [149.91], "Focus": [16657.7], "XYStage": [ 71629.7, 19152.9 ] }, "GridColumnIndex": 0 }, { "Label": "Pos1", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [190.82], "Focus": [16485.4], "XYStage": [ 65702.3, 19396.600000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos2", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [194.69], "Focus": [16446.8], "XYStage": [ 59880.5, 19007.8 ] }, "GridColumnIndex": 0 }, { "Label": "Pos3", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [145.04], "Focus": [16446.6], "XYStage": [ 53616.9, 18818.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos4", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [169.57500000000002], "Focus": [16446.5], "XYStage": [ 47662.8, 18817.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos5", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [131.805], "Focus": [16446.4], "XYStage": [ 41918.8, 18298.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos6", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [113.145], "Focus": [16446.5], "XYStage": [ 36129.9, 18895.8 ] }, "GridColumnIndex": 0 }, { "Label": "Pos7", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [141.005], "Focus": [16446.6], "XYStage": [ 30299.100000000002, 18895.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos8", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [140.97], "Focus": [16446.5], "XYStage": [ 23952.100000000002, 19156 ] }, "GridColumnIndex": 0 }, { "Label": "Pos9", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [184.57500000000002], "Focus": [16677.9], "XYStage": [ 72866.8, -14809.300000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos10", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [167.95000000000002], "Focus": [16557.9], "XYStage": [ 66515.6, -14371.300000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos11", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [168], "Focus": [16618.9], "XYStage": [ 59724.3, -13964.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos12", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [203.205], "Focus": [16779.6], "XYStage": [ 53862.3, -13494.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos13", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [203.20000000000002], "Focus": [16749], "XYStage": [ 48108.3, -13805.400000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos14", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [203.19], "Focus": [16675.9], "XYStage": [ 41645, -13804.900000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos15", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [203.21], "Focus": [16675.7], "XYStage": [ 35352.6, -13805.6 ] }, "GridColumnIndex": 0 }, { "Label": "Pos16", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [203.225], "Focus": [16675.8], "XYStage": [ 29298, -13447.400000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos17", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [203.215], "Focus": [16675.8], "XYStage": [ 23269.100000000002, -13012.300000000001 ] }, "GridColumnIndex": 0 } ], "z-step_um": 10, "GridRow": 0, "BitDepth": 16, "Depth": 2, "Channels": 6, "Source": "Micro-Manager", "ChColors": [ -16711681, -256, -65281, -1, -1, -16776961 ], "MetadataVersion": 10, "KeepShutterOpenChannels": true, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536, 65536 ], "CustomIntervals_ms": [], "Height": 1200, "MicroManagerVersion": "1.4.23 20180219", "GridColumn": 0, "UUID": "3ad8801b-5699-40d6-813b-4ad840863e38", "PixelType": "GRAY16", "Width": 1200, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\Yan\\20191216-YC113-S11S12-miseq", "Frames": 1, "Comment": "", "UserName": "Nikon", "PixelSize_um": 1.1, "ROI": [ 0, 0, 1200, 1200 ], "Date": "2019-12-17", "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC", "P-DAPI-high" ], "KeepShutterOpenSlices": true, "IJType": 1, "ComputerName": "ZadorConfocal2", "Positions": 18, "PositionIndex": 17, "CameraTimeout": 20000, "TimeFirst": false }, "640 AO-Name": "AnalogIO", "Vincent-D3-Command": "Close" }, "FrameKey-0-5-2": { "COM4-StopBits": "1", "528 AO-MaxVolts": "5.0000", "XLIGHT Excitation Wheel-State": "1", "470 Shutter-Name": "AnalogIO", "Core-Shutter": "405", "555 AO-Demo": "No", "XLIGHT Dichroic-State": "2", "XYStage-StepSize-Y": "0.1000", "ZPositionUm": 173.215, "XYStage-StepSize-X": "0.1000", "PRIME-FirmwareVersion": "20.28", "PRIME-ClearCycles": "2", "520-Name": "DA Shutter", "405 Shutter-Name": "AnalogIO", "PIEZO-StepSize": "0.0050", "PRIME-ClearMode": "Pre-Exposure", "470 AO-MinVolts": "0.0000", "405 AO-SupportsTriggering": "1", "CameraChannelIndex": 0, "XYStage-StartSpeed": "500.0000", "COM9-StopBits": "2", "405 AO-Description": "NI DAC", "COM4-DelayBetweenCharsMs": "0.0000", "445 AO-Block voltage": "false", "520 AO-TriggerInputLine": "/Dev1/PFI0", "555 AO-Description": "NI DAC", "520 AO-TriggerSampleRate": "100000", "COM4-Verbose": "1", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM4-Name": "COM4", "405 Shutter-Volts": "5.0000", "Vincent-D3-Port": "COM1", "PRIME-PP 4 MIN ADU AFFECTED": "200", "470 Shutter-MinVolts": "0.0000", "PRIME-PP 3 ENABLED": "Yes", "470 AO-Description": "NI DAC", "PRIME-Description": "PVCAM API device adapter", "520 Shutter-MaxVolts": "5.0000", "520 Shutter-Volts": "0.0000", "528 AO-Sequenceable": "1", "PRIME-Binning": "1x1", "555 Shutter-MaxVolts": "5.0000", "528 Shutter-TriggerSequenceLength": "1000", "405 Shutter-Block voltage": "false", "XLIGHT Spin motor-State": "1", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "Vincent-D3-Time to open (ms)": "35.0000", "Vincent-D3-Time to close (ms)": "35.0000", "445 AO-TriggerSampleRate": "100000", "640 Shutter-IOChannel": "Dev1/ao14", "555 AO-Sequenceable": "1", "520 AO-Name": "AnalogIO", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "470 AO-Block voltage": "false", "COM1-Verbose": "1", "COM9-AnswerTimeout": "10000.0000", "528 Shutter-Name": "AnalogIO", "PRIME-Trigger Timeout (secs)": "10", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "NextFrame": 0, "470 AO-TriggerSequenceLength": "1000", "PRIME-TransposeXY": "0", "445 AO-TriggerSequenceLength": "1000", "COM1-Name": "COM1", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "COM9-DataBits": "8", "COM1-AnswerTimeout": "500.0000", "Camera": "", "TE2000-Version": "V2.50-V2.50-V2.10", "528 Shutter-Volts": "0.0000", "XLIGHT Hub-Name": "XLIGHT Hub", "PIEZO-Description": "Ludl stage driver adapter", "PositionName": "Pos17", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSampleRate": "100000", "PRIME-FanSpeedSetpoint": "High", "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "405 AO-TriggerSampleRate": "100000", "470 Shutter-Block voltage": "false", "470-State": "0", "COM3-Parity": "None", "405 AO-Volts": "4.5000", "PRIME-TransposeMirrorY": "0", "PRIME-ChipName": "GS144BSI", "470 AO-Volts": "0.0000", "PRIME-TransposeMirrorX": "0", "445 Shutter-SupportsTriggering": "0", "470 Shutter-SupportsTriggering": "0", "XLIGHT Hub-Description": "XLIGHT hub", "PRIME-PreampOffLimit": "10000", "470 AO-Sequenceable": "1", "528 Shutter-MaxVolts": "5.0000", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "Core-XYStage": "XYStage", "470 AO-TriggerSampleRate": "100000", "PRIME-Timing-PreTriggerDelayNs": "24672000.0000", "528-DA Device": "528 Shutter", "Focus-Description": "Nikon TE2000 focus adapter", "FilterBlock-State": "5", "520 AO-Demo": "No", "COM3-DelayBetweenCharsMs": "0.0000", "470 Shutter-Volts": "0.0000", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "PRIME-PP 2 THRESHOLD": "125", "PRIME-PP 4 THRESHOLD": "75", "XYStage-Acceleration": "4.0000", "470 Shutter-Demo": "No", "PRIME-Name": "PMPCIECam00", "640-DA Device": "640 Shutter", "405 AO-Block voltage": "false", "ElapsedTime-ms": 749208, "PRIME-Offset": "100", "445 AO-IOChannel": "Dev1/ao1", "640 AO-MaxVolts": "5.0000", "PositionIndex": 17, "PRIME-PP 1 ENABLED": "Yes", "COM1-BaudRate": "9600", "640-State": "0", "COM3-Verbose": "1", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "LudlController-TransmissionDelay": "0", "PRIME-FullWellCapacity": "62000", "PRIME-SMARTStreamingEnabled": "No", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "640 Shutter-Description": "NI DAC", "528-Description": "DA device that is used as a shutter", "COM4-Description": "Serial port driver (boost:asio)", "405 Shutter-Description": "NI DAC", "TE2000-Name": "TE2000", "528 Shutter-Description": "NI DAC", "COM9-Description": "Serial port driver (boost:asio)", "LudlController-Name": "LudlController", "445 Shutter-TriggerSequenceLength": "1000", "PRIME-SerialNumber": "A18B203008", "445 AO-Volts": "0.0000", "PRIME-X-dimension": "1200", "PRIME-ShutterCloseDelay": "0", "XLIGHT Disk slider-State": "2", "Position": "Default", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 AO-IOChannel": "Dev1/ao5", "520 AO-IOChannel": "Dev1/ao4", "528 AO-TriggerSequenceLength": "1000", "405 Shutter-MaxVolts": "5.0000", "470-DA Device": "470 Shutter", "PRIME-FTCapable": "No", "Core-ChannelGroup": "Channels", "555 AO-SupportsTriggering": "0", "PRIME-Y-dimension": "1200", "Frame": 0, "555 Shutter-Sequenceable": "1", "PRIME-Timing-ExposureTimeNs": "499998000.0000", "Core-ImageProcessor": "", "528-State": "0", "520 AO-Block voltage": "false", "XLIGHT Excitation Wheel-Label": "QUAD", "640 Shutter-Demo": "No", "COM3-StopBits": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "520 Shutter-IOChannel": "Dev1/ao11", "Width": 1200, "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-SupportsTriggering": "0", "640 AO-TriggerSampleRate": "100000", "520-Description": "DA device that is used as a shutter", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "405 Shutter-Sequenceable": "1", "405 Shutter-SupportsTriggering": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-TriggerSampleRate": "100000", "640 Shutter-MaxVolts": "5.0000", "555 AO-MaxVolts": "5.0000", "520 AO-TriggerSequenceLength": "1000", "COM3-BaudRate": "9600", "COM9-BaudRate": "9600", "Vincent-D3-Channel #": "Ch. #1", "PRIME-PMode": "Normal", "OpticalPath-Label": "Left(100)", "BitDepth": 16, "555 AO-TriggerSequenceLength": "1000", "445 Shutter-Description": "NI DAC", "405 AO-Sequenceable": "1", "PRIME-AcquisitionMethod": "Callbacks", "555 AO-Name": "AnalogIO", "PRIME-ShutterMode": "Pre-Exposure", "COM3-Handshaking": "Off", "445 AO-SupportsTriggering": "0", "ROI": "0-0-1200-1200", "COM9-Verbose": "1", "PRIME-Color": "OFF", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "PRIME-ShutterOpenDelay": "0", "COM1-Parity": "None", "Slice": 2, "SlicePosition": -30, "COM9-DelayBetweenCharsMs": "0.0000", "555-Name": "DA Shutter", "PIEZO-LudlSingleAxisName": "B", "XLIGHT Emission Wheel-Label": "QUAD", "640 Shutter-Name": "AnalogIO", "640 AO-Sequenceable": "1", "520 Shutter-Demo": "No", "528 Shutter-Demo": "No", "470-Name": "DA Shutter", "520-DA Device": "520 Shutter", "XYStage-Description": "Ludl XY stage driver adapter", "445 Shutter-MinVolts": "0.0000", "528 AO-TriggerSampleRate": "100000", "405 AO-MinVolts": "0.0000", "405 Shutter-TriggerSequenceLength": "1000", "Height": 1200, "555 AO-Block voltage": "false", "640 Shutter-Volts": "0.0000", "PixelType": "GRAY16", "445 Shutter-Block voltage": "false", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "520 Shutter-TriggerSequenceLength": "1000", "COM9-Name": "COM9", "Core-SLM": "", "555 AO-Volts": "0.0000", "555 Shutter-Block voltage": "false", "XYStage-Speed": "1800.0000", "520 AO-MaxVolts": "5.0000", "528 Shutter-SupportsTriggering": "0", "COM1-Description": "Serial port driver (boost:asio)", "PRIME-MetadataEnabled": "No", "520 Shutter-MinVolts": "0.0000", "470 Shutter-Sequenceable": "1", "PRIME-PreampDelay": "0", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-MinVolts": "0.0000", "ChannelIndex": 5, "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Core-AutoShutter": "1", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "Exposure-ms": 300, "PRIME-PP 2 ENABLED": "Yes", "640 Shutter-TriggerSampleRate": "100000", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "470 Shutter-IOChannel": "Dev1/ao10", "405 AO-Demo": "No", "PixelSizeUm": 1.1, "470 AO-Demo": "No", "470 Shutter-MaxVolts": "5.0000", "Vincent-D3-Name": "Vincent-D3", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FileName": "img_000000000_P-DAPI-high_002.tif", "LudlController-Reset": "Operate", "PRIME-Exposure": "300.00", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "445-Name": "DA Shutter", "555 Shutter-Name": "AnalogIO", "520 Shutter-TriggerSampleRate": "100000", "445 Shutter-IOChannel": "Dev1/ao9", "PRIME-TransposeCorrection": "0", "XYStage-StepSize": "0.1000", "445-Description": "DA device that is used as a shutter", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 AO-IOChannel": "Dev1/ao7", "520 Shutter-Name": "AnalogIO", "COM3-DataBits": "8", "640 AO-Volts": "0.0000", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "555 Shutter-IOChannel": "Dev1/ao13", "WaitInterval": null, "520 Shutter-Sequenceable": "1", "YPositionUm": -13012.300000000001, "445-State": "0", "445-DA Device": "445 Shutter", "PRIME-CircularBufferAutoSize": "ON", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "555 AO-TriggerSampleRate": "100000", "445 Shutter-Volts": "0.0000", "XLIGHT Hub-Port": "COM3", "Core-AutoFocus": "", "COM4-Handshaking": "Off", "528 Shutter-MinVolts": "0.0000", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "445 Shutter-Name": "AnalogIO", "528 Shutter-Sequenceable": "1", "XYStage-TransposeMirrorX": "0", "XYStage-TransposeMirrorY": "0", "PRIME-ReadoutRate": "100MHz 16bit", "XYStage-Name": "Stage", "640 AO-SupportsTriggering": "0", "640-Name": "DA Shutter", "640 Shutter-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "640 AO-Block voltage": "false", "445 Shutter-Sequenceable": "1", "470 AO-MaxVolts": "5.0000", "COM4-BaudRate": "9600", "470-Description": "DA device that is used as a shutter", "520 Shutter-Description": "NI DAC", "PRIME-CCDTemperature": "-14.9200", "Time": "2019-12-17 04:00:06 -0500", "640 AO-TriggerSequenceLength": "1000", "470 AO-Name": "AnalogIO", "XLIGHT Disk slider-Label": "Disk Pos 40um", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "Binning": "1x1", "TE2000-ModelType": "1", "COM3-AnswerTimeout": "500.0000", "555 Shutter-SupportsTriggering": "0", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "TE2000-Port": "COM4", "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Description": "NI DAC", "COM4-AnswerTimeout": "500.0000", "COM1-DelayBetweenCharsMs": "0.0000", "FilterBlock-Label": "Confocal", "445 AO-Name": "AnalogIO", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-Timing-ReadoutTimeNs": "24672000.0000", "528 AO-Demo": "No", "520 AO-Sequenceable": "1", "555-Description": "DA device that is used as a shutter", "405-DA Device": "405 Shutter", "405 AO-TriggerSequenceLength": "1000", "640 AO-MinVolts": "0.0000", "640 Shutter-Block voltage": "false", "FrameIndex": 0, "PRIME-Port": "CMOS", "555 AO-MinVolts": "0.0000", "COM3-Description": "Serial port driver (boost:asio)", "528 AO-Description": "NI DAC", "555 AO-IOChannel": "Dev1/ao6", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "555 AO-TriggerInputLine": "/Dev1/PFI0", "PRIME-ActualInterval-ms": "84.6200", "LudlController-Port": "COM9", "445 Shutter-Demo": "No", "PRIME-Trigger-Expose Out-Mux": "1", "SliceIndex": 2, "PRIME-CCDTemperatureSetPoint": "-15.0000", "405 Shutter-MinVolts": "0.0000", "PRIME-ExposeOutMode": "First Row", "AxisPositions": null, "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Volts": "0.0000", "528-Name": "DA Shutter", "555-State": "0", "XLIGHT Dichroic-Label": "QUAD", "445 AO-Sequenceable": "1", "405-Name": "DA Shutter", "405 AO-IOChannel": "Dev1/ao0", "Core-Camera": "PRIME", "Focus-Name": "Focus", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "405 Shutter-IOChannel": "Dev1/ao8", "640 AO-Demo": "No", "640 Shutter-SupportsTriggering": "0", "445 AO-Demo": "No", "555-DA Device": "555 Shutter", "470 Shutter-Description": "NI DAC", "528 AO-Volts": "0.0000", "COM1-DataBits": "8", "Vincent-D3-Address": "x", "520 AO-SupportsTriggering": "0", "405-Description": "DA device that is used as a shutter", "640 Shutter-MinVolts": "0.0000", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "405 AO-Name": "AnalogIO", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "640 AO-TriggerInputLine": "/Dev1/PFI0", "405 AO-TriggerInputLine": "/Dev1/PFI0", "640-Description": "DA device that is used as a shutter", "445 Shutter-TriggerSampleRate": "100000", "470 Shutter-TriggerSequenceLength": "1000", "COM1-StopBits": "1", "520 Shutter-SupportsTriggering": "0", "405-State": "1", "Core-Initialize": "1", "XLIGHT Emission Wheel-State": "3", "520 AO-Description": "NI DAC", "528 AO-TriggerInputLine": "/Dev1/PFI0", "445 Shutter-MaxVolts": "5.0000", "COM9-Handshaking": "Off", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "XLIGHT Spin motor-Label": "On (spinning)", "PRIME-PP 5 ENABLED": "No", "UUID": "f4199d6a-7442-4e97-b77a-49e365885a68", "PRIME-TriggerMode": "Internal Trigger", "COM1-Handshaking": "Off", "PRIME-Timing-PostTriggerDelayNs": "49344000.0000", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "Focus-Position": "16732.9000", "528 Shutter-Block voltage": "false", "Channel": "P-DAPI-high", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "555 Shutter-Demo": "No", "405 Shutter-Demo": "No", "528 AO-Name": "AnalogIO", "520-State": "0", "Core-Galvo": "", "405 Shutter-TriggerSampleRate": "100000", "Focus-FocusStepSize_(nm)": "100", "PRIME-PixelType": "16bit", "COM4-DataBits": "8", "470 AO-IOChannel": "Dev1/ao2", "520 AO-MinVolts": "0.0000", "COM9-Parity": "None", "PRIME-CircularBufferEnabled": "ON", "PRIME-PP 6 Reset": "No", "640 AO-Description": "NI DAC", "Source": "PRIME", "445 AO-MinVolts": "0.0000", "PIEZO-Name": "Stage", "445 AO-Description": "NI DAC", "PRIME-CircularBufferFrameCount": "46", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "520 AO-Volts": "0.0000", "528 Shutter-TriggerSampleRate": "100000", "XPositionUm": 23269.100000000002, "OpticalPath-State": "4", "PRIME-PP 3 THRESHOLD": "80", "PRIME-PVCAM Version": "3.7.1", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Gain": "1-HDR", "640 Shutter-TriggerSequenceLength": "1000", "528 AO-SupportsTriggering": "0", "445 AO-TriggerInputLine": "/Dev1/PFI0", "OpticalPath-Name": "OpticalPath", "PRIME-PP 4 ENABLED": "Yes", "PRIME-PVCAM Adapter Version": "1.3.29", "COM3-Name": "COM3", "405 AO-MaxVolts": "5.0000", "520 Shutter-Block voltage": "false", "PRIME-Timing-ClearingTimeNs": "49344000.0000", "Core-Focus": "PIEZO", "528 AO-Block voltage": "false", "TE2000-Description": "Nikon TE2000 hub", "528 AO-MinVolts": "0.0000", "COM4-Parity": "None", "Summary": { "Slices": 11, "ChContrastMin": [ 0, 0, 0, 0, 0, 0 ], "PixelAspect": 1, "SlicesFirst": true, "Interval_ms": 0, "Time": "2019-12-17 03:59:33 -0500", "Prefix": "seq_7", "InitialPositionList": [ { "Label": "Pos0", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [149.91], "Focus": [16657.7], "XYStage": [ 71629.7, 19152.9 ] }, "GridColumnIndex": 0 }, { "Label": "Pos1", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [190.82], "Focus": [16485.4], "XYStage": [ 65702.3, 19396.600000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos2", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [194.69], "Focus": [16446.8], "XYStage": [ 59880.5, 19007.8 ] }, "GridColumnIndex": 0 }, { "Label": "Pos3", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [145.04], "Focus": [16446.6], "XYStage": [ 53616.9, 18818.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos4", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [169.57500000000002], "Focus": [16446.5], "XYStage": [ 47662.8, 18817.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos5", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [131.805], "Focus": [16446.4], "XYStage": [ 41918.8, 18298.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos6", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [113.145], "Focus": [16446.5], "XYStage": [ 36129.9, 18895.8 ] }, "GridColumnIndex": 0 }, { "Label": "Pos7", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [141.005], "Focus": [16446.6], "XYStage": [ 30299.100000000002, 18895.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos8", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [140.97], "Focus": [16446.5], "XYStage": [ 23952.100000000002, 19156 ] }, "GridColumnIndex": 0 }, { "Label": "Pos9", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [184.57500000000002], "Focus": [16677.9], "XYStage": [ 72866.8, -14809.300000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos10", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [167.95000000000002], "Focus": [16557.9], "XYStage": [ 66515.6, -14371.300000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos11", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [168], "Focus": [16618.9], "XYStage": [ 59724.3, -13964.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos12", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [203.205], "Focus": [16779.6], "XYStage": [ 53862.3, -13494.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos13", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [203.20000000000002], "Focus": [16749], "XYStage": [ 48108.3, -13805.400000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos14", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [203.19], "Focus": [16675.9], "XYStage": [ 41645, -13804.900000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos15", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [203.21], "Focus": [16675.7], "XYStage": [ 35352.6, -13805.6 ] }, "GridColumnIndex": 0 }, { "Label": "Pos16", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [203.225], "Focus": [16675.8], "XYStage": [ 29298, -13447.400000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos17", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [203.215], "Focus": [16675.8], "XYStage": [ 23269.100000000002, -13012.300000000001 ] }, "GridColumnIndex": 0 } ], "z-step_um": 10, "GridRow": 0, "BitDepth": 16, "Depth": 2, "Channels": 6, "Source": "Micro-Manager", "ChColors": [ -16711681, -256, -65281, -1, -1, -16776961 ], "MetadataVersion": 10, "KeepShutterOpenChannels": true, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536, 65536 ], "CustomIntervals_ms": [], "Height": 1200, "MicroManagerVersion": "1.4.23 20180219", "GridColumn": 0, "UUID": "3ad8801b-5699-40d6-813b-4ad840863e38", "PixelType": "GRAY16", "Width": 1200, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\Yan\\20191216-YC113-S11S12-miseq", "Frames": 1, "Comment": "", "UserName": "Nikon", "PixelSize_um": 1.1, "ROI": [ 0, 0, 1200, 1200 ], "Date": "2019-12-17", "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC", "P-DAPI-high" ], "KeepShutterOpenSlices": true, "IJType": 1, "ComputerName": "ZadorConfocal2", "Positions": 18, "PositionIndex": 17, "CameraTimeout": 20000, "TimeFirst": false }, "640 AO-Name": "AnalogIO", "Vincent-D3-Command": "Close" }, "FrameKey-0-5-3": { "COM4-StopBits": "1", "528 AO-MaxVolts": "5.0000", "XLIGHT Excitation Wheel-State": "1", "470 Shutter-Name": "AnalogIO", "Core-Shutter": "405", "555 AO-Demo": "No", "XLIGHT Dichroic-State": "2", "XYStage-StepSize-Y": "0.1000", "ZPositionUm": 183.215, "XYStage-StepSize-X": "0.1000", "PRIME-FirmwareVersion": "20.28", "PRIME-ClearCycles": "2", "520-Name": "DA Shutter", "405 Shutter-Name": "AnalogIO", "PIEZO-StepSize": "0.0050", "PRIME-ClearMode": "Pre-Exposure", "470 AO-MinVolts": "0.0000", "405 AO-SupportsTriggering": "1", "CameraChannelIndex": 0, "XYStage-StartSpeed": "500.0000", "COM9-StopBits": "2", "405 AO-Description": "NI DAC", "COM4-DelayBetweenCharsMs": "0.0000", "445 AO-Block voltage": "false", "520 AO-TriggerInputLine": "/Dev1/PFI0", "555 AO-Description": "NI DAC", "520 AO-TriggerSampleRate": "100000", "COM4-Verbose": "1", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM4-Name": "COM4", "405 Shutter-Volts": "5.0000", "Vincent-D3-Port": "COM1", "PRIME-PP 4 MIN ADU AFFECTED": "200", "470 Shutter-MinVolts": "0.0000", "PRIME-PP 3 ENABLED": "Yes", "470 AO-Description": "NI DAC", "PRIME-Description": "PVCAM API device adapter", "520 Shutter-MaxVolts": "5.0000", "520 Shutter-Volts": "0.0000", "528 AO-Sequenceable": "1", "PRIME-Binning": "1x1", "555 Shutter-MaxVolts": "5.0000", "528 Shutter-TriggerSequenceLength": "1000", "405 Shutter-Block voltage": "false", "XLIGHT Spin motor-State": "1", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "Vincent-D3-Time to open (ms)": "35.0000", "Vincent-D3-Time to close (ms)": "35.0000", "445 AO-TriggerSampleRate": "100000", "640 Shutter-IOChannel": "Dev1/ao14", "555 AO-Sequenceable": "1", "520 AO-Name": "AnalogIO", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "470 AO-Block voltage": "false", "COM1-Verbose": "1", "COM9-AnswerTimeout": "10000.0000", "528 Shutter-Name": "AnalogIO", "PRIME-Trigger Timeout (secs)": "10", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "NextFrame": 0, "470 AO-TriggerSequenceLength": "1000", "PRIME-TransposeXY": "0", "445 AO-TriggerSequenceLength": "1000", "COM1-Name": "COM1", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "COM9-DataBits": "8", "COM1-AnswerTimeout": "500.0000", "Camera": "", "TE2000-Version": "V2.50-V2.50-V2.10", "528 Shutter-Volts": "0.0000", "XLIGHT Hub-Name": "XLIGHT Hub", "PIEZO-Description": "Ludl stage driver adapter", "PositionName": "Pos17", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSampleRate": "100000", "PRIME-FanSpeedSetpoint": "High", "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "405 AO-TriggerSampleRate": "100000", "470 Shutter-Block voltage": "false", "470-State": "0", "COM3-Parity": "None", "405 AO-Volts": "4.5000", "PRIME-TransposeMirrorY": "0", "PRIME-ChipName": "GS144BSI", "470 AO-Volts": "0.0000", "PRIME-TransposeMirrorX": "0", "445 Shutter-SupportsTriggering": "0", "470 Shutter-SupportsTriggering": "0", "XLIGHT Hub-Description": "XLIGHT hub", "PRIME-PreampOffLimit": "10000", "470 AO-Sequenceable": "1", "528 Shutter-MaxVolts": "5.0000", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "Core-XYStage": "XYStage", "470 AO-TriggerSampleRate": "100000", "PRIME-Timing-PreTriggerDelayNs": "24672000.0000", "528-DA Device": "528 Shutter", "Focus-Description": "Nikon TE2000 focus adapter", "FilterBlock-State": "5", "520 AO-Demo": "No", "COM3-DelayBetweenCharsMs": "0.0000", "470 Shutter-Volts": "0.0000", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "PRIME-PP 2 THRESHOLD": "125", "PRIME-PP 4 THRESHOLD": "75", "XYStage-Acceleration": "4.0000", "470 Shutter-Demo": "No", "PRIME-Name": "PMPCIECam00", "640-DA Device": "640 Shutter", "405 AO-Block voltage": "false", "ElapsedTime-ms": 749763, "PRIME-Offset": "100", "445 AO-IOChannel": "Dev1/ao1", "640 AO-MaxVolts": "5.0000", "PositionIndex": 17, "PRIME-PP 1 ENABLED": "Yes", "COM1-BaudRate": "9600", "640-State": "0", "COM3-Verbose": "1", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "LudlController-TransmissionDelay": "0", "PRIME-FullWellCapacity": "62000", "PRIME-SMARTStreamingEnabled": "No", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "640 Shutter-Description": "NI DAC", "528-Description": "DA device that is used as a shutter", "COM4-Description": "Serial port driver (boost:asio)", "405 Shutter-Description": "NI DAC", "TE2000-Name": "TE2000", "528 Shutter-Description": "NI DAC", "COM9-Description": "Serial port driver (boost:asio)", "LudlController-Name": "LudlController", "445 Shutter-TriggerSequenceLength": "1000", "PRIME-SerialNumber": "A18B203008", "445 AO-Volts": "0.0000", "PRIME-X-dimension": "1200", "PRIME-ShutterCloseDelay": "0", "XLIGHT Disk slider-State": "2", "Position": "Default", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 AO-IOChannel": "Dev1/ao5", "520 AO-IOChannel": "Dev1/ao4", "528 AO-TriggerSequenceLength": "1000", "405 Shutter-MaxVolts": "5.0000", "470-DA Device": "470 Shutter", "PRIME-FTCapable": "No", "Core-ChannelGroup": "Channels", "555 AO-SupportsTriggering": "0", "PRIME-Y-dimension": "1200", "Frame": 0, "555 Shutter-Sequenceable": "1", "PRIME-Timing-ExposureTimeNs": "499998000.0000", "Core-ImageProcessor": "", "528-State": "0", "520 AO-Block voltage": "false", "XLIGHT Excitation Wheel-Label": "QUAD", "640 Shutter-Demo": "No", "COM3-StopBits": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "520 Shutter-IOChannel": "Dev1/ao11", "Width": 1200, "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-SupportsTriggering": "0", "640 AO-TriggerSampleRate": "100000", "520-Description": "DA device that is used as a shutter", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "405 Shutter-Sequenceable": "1", "405 Shutter-SupportsTriggering": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-TriggerSampleRate": "100000", "640 Shutter-MaxVolts": "5.0000", "555 AO-MaxVolts": "5.0000", "520 AO-TriggerSequenceLength": "1000", "COM3-BaudRate": "9600", "COM9-BaudRate": "9600", "Vincent-D3-Channel #": "Ch. #1", "PRIME-PMode": "Normal", "OpticalPath-Label": "Left(100)", "BitDepth": 16, "555 AO-TriggerSequenceLength": "1000", "445 Shutter-Description": "NI DAC", "405 AO-Sequenceable": "1", "PRIME-AcquisitionMethod": "Callbacks", "555 AO-Name": "AnalogIO", "PRIME-ShutterMode": "Pre-Exposure", "COM3-Handshaking": "Off", "445 AO-SupportsTriggering": "0", "ROI": "0-0-1200-1200", "COM9-Verbose": "1", "PRIME-Color": "OFF", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "PRIME-ShutterOpenDelay": "0", "COM1-Parity": "None", "Slice": 3, "SlicePosition": -20, "COM9-DelayBetweenCharsMs": "0.0000", "555-Name": "DA Shutter", "PIEZO-LudlSingleAxisName": "B", "XLIGHT Emission Wheel-Label": "QUAD", "640 Shutter-Name": "AnalogIO", "640 AO-Sequenceable": "1", "520 Shutter-Demo": "No", "528 Shutter-Demo": "No", "470-Name": "DA Shutter", "520-DA Device": "520 Shutter", "XYStage-Description": "Ludl XY stage driver adapter", "445 Shutter-MinVolts": "0.0000", "528 AO-TriggerSampleRate": "100000", "405 AO-MinVolts": "0.0000", "405 Shutter-TriggerSequenceLength": "1000", "Height": 1200, "555 AO-Block voltage": "false", "640 Shutter-Volts": "0.0000", "PixelType": "GRAY16", "445 Shutter-Block voltage": "false", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "520 Shutter-TriggerSequenceLength": "1000", "COM9-Name": "COM9", "Core-SLM": "", "555 AO-Volts": "0.0000", "555 Shutter-Block voltage": "false", "XYStage-Speed": "1800.0000", "520 AO-MaxVolts": "5.0000", "528 Shutter-SupportsTriggering": "0", "COM1-Description": "Serial port driver (boost:asio)", "PRIME-MetadataEnabled": "No", "520 Shutter-MinVolts": "0.0000", "470 Shutter-Sequenceable": "1", "PRIME-PreampDelay": "0", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-MinVolts": "0.0000", "ChannelIndex": 5, "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Core-AutoShutter": "1", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "Exposure-ms": 300, "PRIME-PP 2 ENABLED": "Yes", "640 Shutter-TriggerSampleRate": "100000", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "470 Shutter-IOChannel": "Dev1/ao10", "405 AO-Demo": "No", "PixelSizeUm": 1.1, "470 AO-Demo": "No", "470 Shutter-MaxVolts": "5.0000", "Vincent-D3-Name": "Vincent-D3", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FileName": "img_000000000_P-DAPI-high_003.tif", "LudlController-Reset": "Operate", "PRIME-Exposure": "300.00", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "445-Name": "DA Shutter", "555 Shutter-Name": "AnalogIO", "520 Shutter-TriggerSampleRate": "100000", "445 Shutter-IOChannel": "Dev1/ao9", "PRIME-TransposeCorrection": "0", "XYStage-StepSize": "0.1000", "445-Description": "DA device that is used as a shutter", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 AO-IOChannel": "Dev1/ao7", "520 Shutter-Name": "AnalogIO", "COM3-DataBits": "8", "640 AO-Volts": "0.0000", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "555 Shutter-IOChannel": "Dev1/ao13", "WaitInterval": null, "520 Shutter-Sequenceable": "1", "YPositionUm": -13012.300000000001, "445-State": "0", "445-DA Device": "445 Shutter", "PRIME-CircularBufferAutoSize": "ON", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "555 AO-TriggerSampleRate": "100000", "445 Shutter-Volts": "0.0000", "XLIGHT Hub-Port": "COM3", "Core-AutoFocus": "", "COM4-Handshaking": "Off", "528 Shutter-MinVolts": "0.0000", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "445 Shutter-Name": "AnalogIO", "528 Shutter-Sequenceable": "1", "XYStage-TransposeMirrorX": "0", "XYStage-TransposeMirrorY": "0", "PRIME-ReadoutRate": "100MHz 16bit", "XYStage-Name": "Stage", "640 AO-SupportsTriggering": "0", "640-Name": "DA Shutter", "640 Shutter-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "640 AO-Block voltage": "false", "445 Shutter-Sequenceable": "1", "470 AO-MaxVolts": "5.0000", "COM4-BaudRate": "9600", "470-Description": "DA device that is used as a shutter", "520 Shutter-Description": "NI DAC", "PRIME-CCDTemperature": "-14.9200", "Time": "2019-12-17 04:00:06 -0500", "640 AO-TriggerSequenceLength": "1000", "470 AO-Name": "AnalogIO", "XLIGHT Disk slider-Label": "Disk Pos 40um", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "Binning": "1x1", "TE2000-ModelType": "1", "COM3-AnswerTimeout": "500.0000", "555 Shutter-SupportsTriggering": "0", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "TE2000-Port": "COM4", "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Description": "NI DAC", "COM4-AnswerTimeout": "500.0000", "COM1-DelayBetweenCharsMs": "0.0000", "FilterBlock-Label": "Confocal", "445 AO-Name": "AnalogIO", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-Timing-ReadoutTimeNs": "24672000.0000", "528 AO-Demo": "No", "520 AO-Sequenceable": "1", "555-Description": "DA device that is used as a shutter", "405-DA Device": "405 Shutter", "405 AO-TriggerSequenceLength": "1000", "640 AO-MinVolts": "0.0000", "640 Shutter-Block voltage": "false", "FrameIndex": 0, "PRIME-Port": "CMOS", "555 AO-MinVolts": "0.0000", "COM3-Description": "Serial port driver (boost:asio)", "528 AO-Description": "NI DAC", "555 AO-IOChannel": "Dev1/ao6", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "555 AO-TriggerInputLine": "/Dev1/PFI0", "PRIME-ActualInterval-ms": "84.6200", "LudlController-Port": "COM9", "445 Shutter-Demo": "No", "PRIME-Trigger-Expose Out-Mux": "1", "SliceIndex": 3, "PRIME-CCDTemperatureSetPoint": "-15.0000", "405 Shutter-MinVolts": "0.0000", "PRIME-ExposeOutMode": "First Row", "AxisPositions": null, "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Volts": "0.0000", "528-Name": "DA Shutter", "555-State": "0", "XLIGHT Dichroic-Label": "QUAD", "445 AO-Sequenceable": "1", "405-Name": "DA Shutter", "405 AO-IOChannel": "Dev1/ao0", "Core-Camera": "PRIME", "Focus-Name": "Focus", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "405 Shutter-IOChannel": "Dev1/ao8", "640 AO-Demo": "No", "640 Shutter-SupportsTriggering": "0", "445 AO-Demo": "No", "555-DA Device": "555 Shutter", "470 Shutter-Description": "NI DAC", "528 AO-Volts": "0.0000", "COM1-DataBits": "8", "Vincent-D3-Address": "x", "520 AO-SupportsTriggering": "0", "405-Description": "DA device that is used as a shutter", "640 Shutter-MinVolts": "0.0000", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "405 AO-Name": "AnalogIO", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "640 AO-TriggerInputLine": "/Dev1/PFI0", "405 AO-TriggerInputLine": "/Dev1/PFI0", "640-Description": "DA device that is used as a shutter", "445 Shutter-TriggerSampleRate": "100000", "470 Shutter-TriggerSequenceLength": "1000", "COM1-StopBits": "1", "520 Shutter-SupportsTriggering": "0", "405-State": "1", "Core-Initialize": "1", "XLIGHT Emission Wheel-State": "3", "520 AO-Description": "NI DAC", "528 AO-TriggerInputLine": "/Dev1/PFI0", "445 Shutter-MaxVolts": "5.0000", "COM9-Handshaking": "Off", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "XLIGHT Spin motor-Label": "On (spinning)", "PRIME-PP 5 ENABLED": "No", "UUID": "5b8a0dbe-ff1f-4a30-aa48-0cde46b43b3a", "PRIME-TriggerMode": "Internal Trigger", "COM1-Handshaking": "Off", "PRIME-Timing-PostTriggerDelayNs": "49344000.0000", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "Focus-Position": "16732.9000", "528 Shutter-Block voltage": "false", "Channel": "P-DAPI-high", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "555 Shutter-Demo": "No", "405 Shutter-Demo": "No", "528 AO-Name": "AnalogIO", "520-State": "0", "Core-Galvo": "", "405 Shutter-TriggerSampleRate": "100000", "Focus-FocusStepSize_(nm)": "100", "PRIME-PixelType": "16bit", "COM4-DataBits": "8", "470 AO-IOChannel": "Dev1/ao2", "520 AO-MinVolts": "0.0000", "COM9-Parity": "None", "PRIME-CircularBufferEnabled": "ON", "PRIME-PP 6 Reset": "No", "640 AO-Description": "NI DAC", "Source": "PRIME", "445 AO-MinVolts": "0.0000", "PIEZO-Name": "Stage", "445 AO-Description": "NI DAC", "PRIME-CircularBufferFrameCount": "46", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "520 AO-Volts": "0.0000", "528 Shutter-TriggerSampleRate": "100000", "XPositionUm": 23269.100000000002, "OpticalPath-State": "4", "PRIME-PP 3 THRESHOLD": "80", "PRIME-PVCAM Version": "3.7.1", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Gain": "1-HDR", "640 Shutter-TriggerSequenceLength": "1000", "528 AO-SupportsTriggering": "0", "445 AO-TriggerInputLine": "/Dev1/PFI0", "OpticalPath-Name": "OpticalPath", "PRIME-PP 4 ENABLED": "Yes", "PRIME-PVCAM Adapter Version": "1.3.29", "COM3-Name": "COM3", "405 AO-MaxVolts": "5.0000", "520 Shutter-Block voltage": "false", "PRIME-Timing-ClearingTimeNs": "49344000.0000", "Core-Focus": "PIEZO", "528 AO-Block voltage": "false", "TE2000-Description": "Nikon TE2000 hub", "528 AO-MinVolts": "0.0000", "COM4-Parity": "None", "Summary": { "Slices": 11, "ChContrastMin": [ 0, 0, 0, 0, 0, 0 ], "PixelAspect": 1, "SlicesFirst": true, "Interval_ms": 0, "Time": "2019-12-17 03:59:33 -0500", "Prefix": "seq_7", "InitialPositionList": [ { "Label": "Pos0", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [149.91], "Focus": [16657.7], "XYStage": [ 71629.7, 19152.9 ] }, "GridColumnIndex": 0 }, { "Label": "Pos1", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [190.82], "Focus": [16485.4], "XYStage": [ 65702.3, 19396.600000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos2", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [194.69], "Focus": [16446.8], "XYStage": [ 59880.5, 19007.8 ] }, "GridColumnIndex": 0 }, { "Label": "Pos3", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [145.04], "Focus": [16446.6], "XYStage": [ 53616.9, 18818.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos4", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [169.57500000000002], "Focus": [16446.5], "XYStage": [ 47662.8, 18817.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos5", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [131.805], "Focus": [16446.4], "XYStage": [ 41918.8, 18298.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos6", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [113.145], "Focus": [16446.5], "XYStage": [ 36129.9, 18895.8 ] }, "GridColumnIndex": 0 }, { "Label": "Pos7", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [141.005], "Focus": [16446.6], "XYStage": [ 30299.100000000002, 18895.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos8", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [140.97], "Focus": [16446.5], "XYStage": [ 23952.100000000002, 19156 ] }, "GridColumnIndex": 0 }, { "Label": "Pos9", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [184.57500000000002], "Focus": [16677.9], "XYStage": [ 72866.8, -14809.300000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos10", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [167.95000000000002], "Focus": [16557.9], "XYStage": [ 66515.6, -14371.300000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos11", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [168], "Focus": [16618.9], "XYStage": [ 59724.3, -13964.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos12", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [203.205], "Focus": [16779.6], "XYStage": [ 53862.3, -13494.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos13", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [203.20000000000002], "Focus": [16749], "XYStage": [ 48108.3, -13805.400000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos14", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [203.19], "Focus": [16675.9], "XYStage": [ 41645, -13804.900000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos15", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [203.21], "Focus": [16675.7], "XYStage": [ 35352.6, -13805.6 ] }, "GridColumnIndex": 0 }, { "Label": "Pos16", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [203.225], "Focus": [16675.8], "XYStage": [ 29298, -13447.400000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos17", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [203.215], "Focus": [16675.8], "XYStage": [ 23269.100000000002, -13012.300000000001 ] }, "GridColumnIndex": 0 } ], "z-step_um": 10, "GridRow": 0, "BitDepth": 16, "Depth": 2, "Channels": 6, "Source": "Micro-Manager", "ChColors": [ -16711681, -256, -65281, -1, -1, -16776961 ], "MetadataVersion": 10, "KeepShutterOpenChannels": true, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536, 65536 ], "CustomIntervals_ms": [], "Height": 1200, "MicroManagerVersion": "1.4.23 20180219", "GridColumn": 0, "UUID": "3ad8801b-5699-40d6-813b-4ad840863e38", "PixelType": "GRAY16", "Width": 1200, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\Yan\\20191216-YC113-S11S12-miseq", "Frames": 1, "Comment": "", "UserName": "Nikon", "PixelSize_um": 1.1, "ROI": [ 0, 0, 1200, 1200 ], "Date": "2019-12-17", "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC", "P-DAPI-high" ], "KeepShutterOpenSlices": true, "IJType": 1, "ComputerName": "ZadorConfocal2", "Positions": 18, "PositionIndex": 17, "CameraTimeout": 20000, "TimeFirst": false }, "640 AO-Name": "AnalogIO", "Vincent-D3-Command": "Close" }, "FrameKey-0-5-4": { "COM4-StopBits": "1", "528 AO-MaxVolts": "5.0000", "XLIGHT Excitation Wheel-State": "1", "470 Shutter-Name": "AnalogIO", "Core-Shutter": "405", "555 AO-Demo": "No", "XLIGHT Dichroic-State": "2", "XYStage-StepSize-Y": "0.1000", "ZPositionUm": 193.215, "XYStage-StepSize-X": "0.1000", "PRIME-FirmwareVersion": "20.28", "PRIME-ClearCycles": "2", "520-Name": "DA Shutter", "405 Shutter-Name": "AnalogIO", "PIEZO-StepSize": "0.0050", "PRIME-ClearMode": "Pre-Exposure", "470 AO-MinVolts": "0.0000", "405 AO-SupportsTriggering": "1", "CameraChannelIndex": 0, "XYStage-StartSpeed": "500.0000", "COM9-StopBits": "2", "405 AO-Description": "NI DAC", "COM4-DelayBetweenCharsMs": "0.0000", "445 AO-Block voltage": "false", "520 AO-TriggerInputLine": "/Dev1/PFI0", "555 AO-Description": "NI DAC", "520 AO-TriggerSampleRate": "100000", "COM4-Verbose": "1", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM4-Name": "COM4", "405 Shutter-Volts": "5.0000", "Vincent-D3-Port": "COM1", "PRIME-PP 4 MIN ADU AFFECTED": "200", "470 Shutter-MinVolts": "0.0000", "PRIME-PP 3 ENABLED": "Yes", "470 AO-Description": "NI DAC", "PRIME-Description": "PVCAM API device adapter", "520 Shutter-MaxVolts": "5.0000", "520 Shutter-Volts": "0.0000", "528 AO-Sequenceable": "1", "PRIME-Binning": "1x1", "555 Shutter-MaxVolts": "5.0000", "528 Shutter-TriggerSequenceLength": "1000", "405 Shutter-Block voltage": "false", "XLIGHT Spin motor-State": "1", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "Vincent-D3-Time to open (ms)": "35.0000", "Vincent-D3-Time to close (ms)": "35.0000", "445 AO-TriggerSampleRate": "100000", "640 Shutter-IOChannel": "Dev1/ao14", "555 AO-Sequenceable": "1", "520 AO-Name": "AnalogIO", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "470 AO-Block voltage": "false", "COM1-Verbose": "1", "COM9-AnswerTimeout": "10000.0000", "528 Shutter-Name": "AnalogIO", "PRIME-Trigger Timeout (secs)": "10", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "NextFrame": 0, "470 AO-TriggerSequenceLength": "1000", "PRIME-TransposeXY": "0", "445 AO-TriggerSequenceLength": "1000", "COM1-Name": "COM1", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "COM9-DataBits": "8", "COM1-AnswerTimeout": "500.0000", "Camera": "", "TE2000-Version": "V2.50-V2.50-V2.10", "528 Shutter-Volts": "0.0000", "XLIGHT Hub-Name": "XLIGHT Hub", "PIEZO-Description": "Ludl stage driver adapter", "PositionName": "Pos17", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSampleRate": "100000", "PRIME-FanSpeedSetpoint": "High", "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "405 AO-TriggerSampleRate": "100000", "470 Shutter-Block voltage": "false", "470-State": "0", "COM3-Parity": "None", "405 AO-Volts": "4.5000", "PRIME-TransposeMirrorY": "0", "PRIME-ChipName": "GS144BSI", "470 AO-Volts": "0.0000", "PRIME-TransposeMirrorX": "0", "445 Shutter-SupportsTriggering": "0", "470 Shutter-SupportsTriggering": "0", "XLIGHT Hub-Description": "XLIGHT hub", "PRIME-PreampOffLimit": "10000", "470 AO-Sequenceable": "1", "528 Shutter-MaxVolts": "5.0000", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "Core-XYStage": "XYStage", "470 AO-TriggerSampleRate": "100000", "PRIME-Timing-PreTriggerDelayNs": "24672000.0000", "528-DA Device": "528 Shutter", "Focus-Description": "Nikon TE2000 focus adapter", "FilterBlock-State": "5", "520 AO-Demo": "No", "COM3-DelayBetweenCharsMs": "0.0000", "470 Shutter-Volts": "0.0000", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "PRIME-PP 2 THRESHOLD": "125", "PRIME-PP 4 THRESHOLD": "75", "XYStage-Acceleration": "4.0000", "470 Shutter-Demo": "No", "PRIME-Name": "PMPCIECam00", "640-DA Device": "640 Shutter", "405 AO-Block voltage": "false", "ElapsedTime-ms": 750302, "PRIME-Offset": "100", "445 AO-IOChannel": "Dev1/ao1", "640 AO-MaxVolts": "5.0000", "PositionIndex": 17, "PRIME-PP 1 ENABLED": "Yes", "COM1-BaudRate": "9600", "640-State": "0", "COM3-Verbose": "1", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "LudlController-TransmissionDelay": "0", "PRIME-FullWellCapacity": "62000", "PRIME-SMARTStreamingEnabled": "No", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "640 Shutter-Description": "NI DAC", "528-Description": "DA device that is used as a shutter", "COM4-Description": "Serial port driver (boost:asio)", "405 Shutter-Description": "NI DAC", "TE2000-Name": "TE2000", "528 Shutter-Description": "NI DAC", "COM9-Description": "Serial port driver (boost:asio)", "LudlController-Name": "LudlController", "445 Shutter-TriggerSequenceLength": "1000", "PRIME-SerialNumber": "A18B203008", "445 AO-Volts": "0.0000", "PRIME-X-dimension": "1200", "PRIME-ShutterCloseDelay": "0", "XLIGHT Disk slider-State": "2", "Position": "Default", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 AO-IOChannel": "Dev1/ao5", "520 AO-IOChannel": "Dev1/ao4", "528 AO-TriggerSequenceLength": "1000", "405 Shutter-MaxVolts": "5.0000", "470-DA Device": "470 Shutter", "PRIME-FTCapable": "No", "Core-ChannelGroup": "Channels", "555 AO-SupportsTriggering": "0", "PRIME-Y-dimension": "1200", "Frame": 0, "555 Shutter-Sequenceable": "1", "PRIME-Timing-ExposureTimeNs": "499998000.0000", "Core-ImageProcessor": "", "528-State": "0", "520 AO-Block voltage": "false", "XLIGHT Excitation Wheel-Label": "QUAD", "640 Shutter-Demo": "No", "COM3-StopBits": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "520 Shutter-IOChannel": "Dev1/ao11", "Width": 1200, "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-SupportsTriggering": "0", "640 AO-TriggerSampleRate": "100000", "520-Description": "DA device that is used as a shutter", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "405 Shutter-Sequenceable": "1", "405 Shutter-SupportsTriggering": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-TriggerSampleRate": "100000", "640 Shutter-MaxVolts": "5.0000", "555 AO-MaxVolts": "5.0000", "520 AO-TriggerSequenceLength": "1000", "COM3-BaudRate": "9600", "COM9-BaudRate": "9600", "Vincent-D3-Channel #": "Ch. #1", "PRIME-PMode": "Normal", "OpticalPath-Label": "Left(100)", "BitDepth": 16, "555 AO-TriggerSequenceLength": "1000", "445 Shutter-Description": "NI DAC", "405 AO-Sequenceable": "1", "PRIME-AcquisitionMethod": "Callbacks", "555 AO-Name": "AnalogIO", "PRIME-ShutterMode": "Pre-Exposure", "COM3-Handshaking": "Off", "445 AO-SupportsTriggering": "0", "ROI": "0-0-1200-1200", "COM9-Verbose": "1", "PRIME-Color": "OFF", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "PRIME-ShutterOpenDelay": "0", "COM1-Parity": "None", "Slice": 4, "SlicePosition": -10, "COM9-DelayBetweenCharsMs": "0.0000", "555-Name": "DA Shutter", "PIEZO-LudlSingleAxisName": "B", "XLIGHT Emission Wheel-Label": "QUAD", "640 Shutter-Name": "AnalogIO", "640 AO-Sequenceable": "1", "520 Shutter-Demo": "No", "528 Shutter-Demo": "No", "470-Name": "DA Shutter", "520-DA Device": "520 Shutter", "XYStage-Description": "Ludl XY stage driver adapter", "445 Shutter-MinVolts": "0.0000", "528 AO-TriggerSampleRate": "100000", "405 AO-MinVolts": "0.0000", "405 Shutter-TriggerSequenceLength": "1000", "Height": 1200, "555 AO-Block voltage": "false", "640 Shutter-Volts": "0.0000", "PixelType": "GRAY16", "445 Shutter-Block voltage": "false", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "520 Shutter-TriggerSequenceLength": "1000", "COM9-Name": "COM9", "Core-SLM": "", "555 AO-Volts": "0.0000", "555 Shutter-Block voltage": "false", "XYStage-Speed": "1800.0000", "520 AO-MaxVolts": "5.0000", "528 Shutter-SupportsTriggering": "0", "COM1-Description": "Serial port driver (boost:asio)", "PRIME-MetadataEnabled": "No", "520 Shutter-MinVolts": "0.0000", "470 Shutter-Sequenceable": "1", "PRIME-PreampDelay": "0", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-MinVolts": "0.0000", "ChannelIndex": 5, "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Core-AutoShutter": "1", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "Exposure-ms": 300, "PRIME-PP 2 ENABLED": "Yes", "640 Shutter-TriggerSampleRate": "100000", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "470 Shutter-IOChannel": "Dev1/ao10", "405 AO-Demo": "No", "PixelSizeUm": 1.1, "470 AO-Demo": "No", "470 Shutter-MaxVolts": "5.0000", "Vincent-D3-Name": "Vincent-D3", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FileName": "img_000000000_P-DAPI-high_004.tif", "LudlController-Reset": "Operate", "PRIME-Exposure": "300.00", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "445-Name": "DA Shutter", "555 Shutter-Name": "AnalogIO", "520 Shutter-TriggerSampleRate": "100000", "445 Shutter-IOChannel": "Dev1/ao9", "PRIME-TransposeCorrection": "0", "XYStage-StepSize": "0.1000", "445-Description": "DA device that is used as a shutter", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 AO-IOChannel": "Dev1/ao7", "520 Shutter-Name": "AnalogIO", "COM3-DataBits": "8", "640 AO-Volts": "0.0000", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "555 Shutter-IOChannel": "Dev1/ao13", "WaitInterval": null, "520 Shutter-Sequenceable": "1", "YPositionUm": -13012.300000000001, "445-State": "0", "445-DA Device": "445 Shutter", "PRIME-CircularBufferAutoSize": "ON", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "555 AO-TriggerSampleRate": "100000", "445 Shutter-Volts": "0.0000", "XLIGHT Hub-Port": "COM3", "Core-AutoFocus": "", "COM4-Handshaking": "Off", "528 Shutter-MinVolts": "0.0000", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "445 Shutter-Name": "AnalogIO", "528 Shutter-Sequenceable": "1", "XYStage-TransposeMirrorX": "0", "XYStage-TransposeMirrorY": "0", "PRIME-ReadoutRate": "100MHz 16bit", "XYStage-Name": "Stage", "640 AO-SupportsTriggering": "0", "640-Name": "DA Shutter", "640 Shutter-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "640 AO-Block voltage": "false", "445 Shutter-Sequenceable": "1", "470 AO-MaxVolts": "5.0000", "COM4-BaudRate": "9600", "470-Description": "DA device that is used as a shutter", "520 Shutter-Description": "NI DAC", "PRIME-CCDTemperature": "-14.9200", "Time": "2019-12-17 04:00:07 -0500", "640 AO-TriggerSequenceLength": "1000", "470 AO-Name": "AnalogIO", "XLIGHT Disk slider-Label": "Disk Pos 40um", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "Binning": "1x1", "TE2000-ModelType": "1", "COM3-AnswerTimeout": "500.0000", "555 Shutter-SupportsTriggering": "0", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "TE2000-Port": "COM4", "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Description": "NI DAC", "COM4-AnswerTimeout": "500.0000", "COM1-DelayBetweenCharsMs": "0.0000", "FilterBlock-Label": "Confocal", "445 AO-Name": "AnalogIO", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-Timing-ReadoutTimeNs": "24672000.0000", "528 AO-Demo": "No", "520 AO-Sequenceable": "1", "555-Description": "DA device that is used as a shutter", "405-DA Device": "405 Shutter", "405 AO-TriggerSequenceLength": "1000", "640 AO-MinVolts": "0.0000", "640 Shutter-Block voltage": "false", "FrameIndex": 0, "PRIME-Port": "CMOS", "555 AO-MinVolts": "0.0000", "COM3-Description": "Serial port driver (boost:asio)", "528 AO-Description": "NI DAC", "555 AO-IOChannel": "Dev1/ao6", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "555 AO-TriggerInputLine": "/Dev1/PFI0", "PRIME-ActualInterval-ms": "84.6200", "LudlController-Port": "COM9", "445 Shutter-Demo": "No", "PRIME-Trigger-Expose Out-Mux": "1", "SliceIndex": 4, "PRIME-CCDTemperatureSetPoint": "-15.0000", "405 Shutter-MinVolts": "0.0000", "PRIME-ExposeOutMode": "First Row", "AxisPositions": null, "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Volts": "0.0000", "528-Name": "DA Shutter", "555-State": "0", "XLIGHT Dichroic-Label": "QUAD", "445 AO-Sequenceable": "1", "405-Name": "DA Shutter", "405 AO-IOChannel": "Dev1/ao0", "Core-Camera": "PRIME", "Focus-Name": "Focus", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "405 Shutter-IOChannel": "Dev1/ao8", "640 AO-Demo": "No", "640 Shutter-SupportsTriggering": "0", "445 AO-Demo": "No", "555-DA Device": "555 Shutter", "470 Shutter-Description": "NI DAC", "528 AO-Volts": "0.0000", "COM1-DataBits": "8", "Vincent-D3-Address": "x", "520 AO-SupportsTriggering": "0", "405-Description": "DA device that is used as a shutter", "640 Shutter-MinVolts": "0.0000", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "405 AO-Name": "AnalogIO", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "640 AO-TriggerInputLine": "/Dev1/PFI0", "405 AO-TriggerInputLine": "/Dev1/PFI0", "640-Description": "DA device that is used as a shutter", "445 Shutter-TriggerSampleRate": "100000", "470 Shutter-TriggerSequenceLength": "1000", "COM1-StopBits": "1", "520 Shutter-SupportsTriggering": "0", "405-State": "1", "Core-Initialize": "1", "XLIGHT Emission Wheel-State": "3", "520 AO-Description": "NI DAC", "528 AO-TriggerInputLine": "/Dev1/PFI0", "445 Shutter-MaxVolts": "5.0000", "COM9-Handshaking": "Off", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "XLIGHT Spin motor-Label": "On (spinning)", "PRIME-PP 5 ENABLED": "No", "UUID": "59cce430-1390-4bc5-92a9-109742aed61f", "PRIME-TriggerMode": "Internal Trigger", "COM1-Handshaking": "Off", "PRIME-Timing-PostTriggerDelayNs": "49344000.0000", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "Focus-Position": "16732.9000", "528 Shutter-Block voltage": "false", "Channel": "P-DAPI-high", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "555 Shutter-Demo": "No", "405 Shutter-Demo": "No", "528 AO-Name": "AnalogIO", "520-State": "0", "Core-Galvo": "", "405 Shutter-TriggerSampleRate": "100000", "Focus-FocusStepSize_(nm)": "100", "PRIME-PixelType": "16bit", "COM4-DataBits": "8", "470 AO-IOChannel": "Dev1/ao2", "520 AO-MinVolts": "0.0000", "COM9-Parity": "None", "PRIME-CircularBufferEnabled": "ON", "PRIME-PP 6 Reset": "No", "640 AO-Description": "NI DAC", "Source": "PRIME", "445 AO-MinVolts": "0.0000", "PIEZO-Name": "Stage", "445 AO-Description": "NI DAC", "PRIME-CircularBufferFrameCount": "46", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "520 AO-Volts": "0.0000", "528 Shutter-TriggerSampleRate": "100000", "XPositionUm": 23269.100000000002, "OpticalPath-State": "4", "PRIME-PP 3 THRESHOLD": "80", "PRIME-PVCAM Version": "3.7.1", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Gain": "1-HDR", "640 Shutter-TriggerSequenceLength": "1000", "528 AO-SupportsTriggering": "0", "445 AO-TriggerInputLine": "/Dev1/PFI0", "OpticalPath-Name": "OpticalPath", "PRIME-PP 4 ENABLED": "Yes", "PRIME-PVCAM Adapter Version": "1.3.29", "COM3-Name": "COM3", "405 AO-MaxVolts": "5.0000", "520 Shutter-Block voltage": "false", "PRIME-Timing-ClearingTimeNs": "49344000.0000", "Core-Focus": "PIEZO", "528 AO-Block voltage": "false", "TE2000-Description": "Nikon TE2000 hub", "528 AO-MinVolts": "0.0000", "COM4-Parity": "None", "Summary": { "Slices": 11, "ChContrastMin": [ 0, 0, 0, 0, 0, 0 ], "PixelAspect": 1, "SlicesFirst": true, "Interval_ms": 0, "Time": "2019-12-17 03:59:33 -0500", "Prefix": "seq_7", "InitialPositionList": [ { "Label": "Pos0", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [149.91], "Focus": [16657.7], "XYStage": [ 71629.7, 19152.9 ] }, "GridColumnIndex": 0 }, { "Label": "Pos1", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [190.82], "Focus": [16485.4], "XYStage": [ 65702.3, 19396.600000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos2", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [194.69], "Focus": [16446.8], "XYStage": [ 59880.5, 19007.8 ] }, "GridColumnIndex": 0 }, { "Label": "Pos3", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [145.04], "Focus": [16446.6], "XYStage": [ 53616.9, 18818.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos4", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [169.57500000000002], "Focus": [16446.5], "XYStage": [ 47662.8, 18817.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos5", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [131.805], "Focus": [16446.4], "XYStage": [ 41918.8, 18298.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos6", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [113.145], "Focus": [16446.5], "XYStage": [ 36129.9, 18895.8 ] }, "GridColumnIndex": 0 }, { "Label": "Pos7", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [141.005], "Focus": [16446.6], "XYStage": [ 30299.100000000002, 18895.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos8", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [140.97], "Focus": [16446.5], "XYStage": [ 23952.100000000002, 19156 ] }, "GridColumnIndex": 0 }, { "Label": "Pos9", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [184.57500000000002], "Focus": [16677.9], "XYStage": [ 72866.8, -14809.300000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos10", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [167.95000000000002], "Focus": [16557.9], "XYStage": [ 66515.6, -14371.300000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos11", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [168], "Focus": [16618.9], "XYStage": [ 59724.3, -13964.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos12", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [203.205], "Focus": [16779.6], "XYStage": [ 53862.3, -13494.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos13", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [203.20000000000002], "Focus": [16749], "XYStage": [ 48108.3, -13805.400000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos14", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [203.19], "Focus": [16675.9], "XYStage": [ 41645, -13804.900000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos15", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [203.21], "Focus": [16675.7], "XYStage": [ 35352.6, -13805.6 ] }, "GridColumnIndex": 0 }, { "Label": "Pos16", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [203.225], "Focus": [16675.8], "XYStage": [ 29298, -13447.400000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos17", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [203.215], "Focus": [16675.8], "XYStage": [ 23269.100000000002, -13012.300000000001 ] }, "GridColumnIndex": 0 } ], "z-step_um": 10, "GridRow": 0, "BitDepth": 16, "Depth": 2, "Channels": 6, "Source": "Micro-Manager", "ChColors": [ -16711681, -256, -65281, -1, -1, -16776961 ], "MetadataVersion": 10, "KeepShutterOpenChannels": true, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536, 65536 ], "CustomIntervals_ms": [], "Height": 1200, "MicroManagerVersion": "1.4.23 20180219", "GridColumn": 0, "UUID": "3ad8801b-5699-40d6-813b-4ad840863e38", "PixelType": "GRAY16", "Width": 1200, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\Yan\\20191216-YC113-S11S12-miseq", "Frames": 1, "Comment": "", "UserName": "Nikon", "PixelSize_um": 1.1, "ROI": [ 0, 0, 1200, 1200 ], "Date": "2019-12-17", "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC", "P-DAPI-high" ], "KeepShutterOpenSlices": true, "IJType": 1, "ComputerName": "ZadorConfocal2", "Positions": 18, "PositionIndex": 17, "CameraTimeout": 20000, "TimeFirst": false }, "640 AO-Name": "AnalogIO", "Vincent-D3-Command": "Close" }, "FrameKey-0-5-5": { "COM4-StopBits": "1", "528 AO-MaxVolts": "5.0000", "XLIGHT Excitation Wheel-State": "1", "470 Shutter-Name": "AnalogIO", "Core-Shutter": "405", "555 AO-Demo": "No", "XLIGHT Dichroic-State": "2", "XYStage-StepSize-Y": "0.1000", "ZPositionUm": 203.215, "XYStage-StepSize-X": "0.1000", "PRIME-FirmwareVersion": "20.28", "PRIME-ClearCycles": "2", "520-Name": "DA Shutter", "405 Shutter-Name": "AnalogIO", "PIEZO-StepSize": "0.0050", "PRIME-ClearMode": "Pre-Exposure", "470 AO-MinVolts": "0.0000", "405 AO-SupportsTriggering": "1", "CameraChannelIndex": 0, "XYStage-StartSpeed": "500.0000", "COM9-StopBits": "2", "405 AO-Description": "NI DAC", "COM4-DelayBetweenCharsMs": "0.0000", "445 AO-Block voltage": "false", "520 AO-TriggerInputLine": "/Dev1/PFI0", "555 AO-Description": "NI DAC", "520 AO-TriggerSampleRate": "100000", "COM4-Verbose": "1", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM4-Name": "COM4", "405 Shutter-Volts": "5.0000", "Vincent-D3-Port": "COM1", "PRIME-PP 4 MIN ADU AFFECTED": "200", "470 Shutter-MinVolts": "0.0000", "PRIME-PP 3 ENABLED": "Yes", "470 AO-Description": "NI DAC", "PRIME-Description": "PVCAM API device adapter", "520 Shutter-MaxVolts": "5.0000", "520 Shutter-Volts": "0.0000", "528 AO-Sequenceable": "1", "PRIME-Binning": "1x1", "555 Shutter-MaxVolts": "5.0000", "528 Shutter-TriggerSequenceLength": "1000", "405 Shutter-Block voltage": "false", "XLIGHT Spin motor-State": "1", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "Vincent-D3-Time to open (ms)": "35.0000", "Vincent-D3-Time to close (ms)": "35.0000", "445 AO-TriggerSampleRate": "100000", "640 Shutter-IOChannel": "Dev1/ao14", "555 AO-Sequenceable": "1", "520 AO-Name": "AnalogIO", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "470 AO-Block voltage": "false", "COM1-Verbose": "1", "COM9-AnswerTimeout": "10000.0000", "528 Shutter-Name": "AnalogIO", "PRIME-Trigger Timeout (secs)": "10", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "NextFrame": 0, "470 AO-TriggerSequenceLength": "1000", "PRIME-TransposeXY": "0", "445 AO-TriggerSequenceLength": "1000", "COM1-Name": "COM1", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "COM9-DataBits": "8", "COM1-AnswerTimeout": "500.0000", "Camera": "", "TE2000-Version": "V2.50-V2.50-V2.10", "528 Shutter-Volts": "0.0000", "XLIGHT Hub-Name": "XLIGHT Hub", "PIEZO-Description": "Ludl stage driver adapter", "PositionName": "Pos17", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSampleRate": "100000", "PRIME-FanSpeedSetpoint": "High", "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "405 AO-TriggerSampleRate": "100000", "470 Shutter-Block voltage": "false", "470-State": "0", "COM3-Parity": "None", "405 AO-Volts": "4.5000", "PRIME-TransposeMirrorY": "0", "PRIME-ChipName": "GS144BSI", "470 AO-Volts": "0.0000", "PRIME-TransposeMirrorX": "0", "445 Shutter-SupportsTriggering": "0", "470 Shutter-SupportsTriggering": "0", "XLIGHT Hub-Description": "XLIGHT hub", "PRIME-PreampOffLimit": "10000", "470 AO-Sequenceable": "1", "528 Shutter-MaxVolts": "5.0000", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "Core-XYStage": "XYStage", "470 AO-TriggerSampleRate": "100000", "PRIME-Timing-PreTriggerDelayNs": "24672000.0000", "528-DA Device": "528 Shutter", "Focus-Description": "Nikon TE2000 focus adapter", "FilterBlock-State": "5", "520 AO-Demo": "No", "COM3-DelayBetweenCharsMs": "0.0000", "470 Shutter-Volts": "0.0000", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "PRIME-PP 2 THRESHOLD": "125", "PRIME-PP 4 THRESHOLD": "75", "XYStage-Acceleration": "4.0000", "470 Shutter-Demo": "No", "PRIME-Name": "PMPCIECam00", "640-DA Device": "640 Shutter", "405 AO-Block voltage": "false", "ElapsedTime-ms": 750845, "PRIME-Offset": "100", "445 AO-IOChannel": "Dev1/ao1", "640 AO-MaxVolts": "5.0000", "PositionIndex": 17, "PRIME-PP 1 ENABLED": "Yes", "COM1-BaudRate": "9600", "640-State": "0", "COM3-Verbose": "1", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "LudlController-TransmissionDelay": "0", "PRIME-FullWellCapacity": "62000", "PRIME-SMARTStreamingEnabled": "No", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "640 Shutter-Description": "NI DAC", "528-Description": "DA device that is used as a shutter", "COM4-Description": "Serial port driver (boost:asio)", "405 Shutter-Description": "NI DAC", "TE2000-Name": "TE2000", "528 Shutter-Description": "NI DAC", "COM9-Description": "Serial port driver (boost:asio)", "LudlController-Name": "LudlController", "445 Shutter-TriggerSequenceLength": "1000", "PRIME-SerialNumber": "A18B203008", "445 AO-Volts": "0.0000", "PRIME-X-dimension": "1200", "PRIME-ShutterCloseDelay": "0", "XLIGHT Disk slider-State": "2", "Position": "Default", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 AO-IOChannel": "Dev1/ao5", "520 AO-IOChannel": "Dev1/ao4", "528 AO-TriggerSequenceLength": "1000", "405 Shutter-MaxVolts": "5.0000", "470-DA Device": "470 Shutter", "PRIME-FTCapable": "No", "Core-ChannelGroup": "Channels", "555 AO-SupportsTriggering": "0", "PRIME-Y-dimension": "1200", "Frame": 0, "555 Shutter-Sequenceable": "1", "PRIME-Timing-ExposureTimeNs": "499998000.0000", "Core-ImageProcessor": "", "528-State": "0", "520 AO-Block voltage": "false", "XLIGHT Excitation Wheel-Label": "QUAD", "640 Shutter-Demo": "No", "COM3-StopBits": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "520 Shutter-IOChannel": "Dev1/ao11", "Width": 1200, "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-SupportsTriggering": "0", "640 AO-TriggerSampleRate": "100000", "520-Description": "DA device that is used as a shutter", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "405 Shutter-Sequenceable": "1", "405 Shutter-SupportsTriggering": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-TriggerSampleRate": "100000", "640 Shutter-MaxVolts": "5.0000", "555 AO-MaxVolts": "5.0000", "520 AO-TriggerSequenceLength": "1000", "COM3-BaudRate": "9600", "COM9-BaudRate": "9600", "Vincent-D3-Channel #": "Ch. #1", "PRIME-PMode": "Normal", "OpticalPath-Label": "Left(100)", "BitDepth": 16, "555 AO-TriggerSequenceLength": "1000", "445 Shutter-Description": "NI DAC", "405 AO-Sequenceable": "1", "PRIME-AcquisitionMethod": "Callbacks", "555 AO-Name": "AnalogIO", "PRIME-ShutterMode": "Pre-Exposure", "COM3-Handshaking": "Off", "445 AO-SupportsTriggering": "0", "ROI": "0-0-1200-1200", "COM9-Verbose": "1", "PRIME-Color": "OFF", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "PRIME-ShutterOpenDelay": "0", "COM1-Parity": "None", "Slice": 5, "SlicePosition": 0, "COM9-DelayBetweenCharsMs": "0.0000", "555-Name": "DA Shutter", "PIEZO-LudlSingleAxisName": "B", "XLIGHT Emission Wheel-Label": "QUAD", "640 Shutter-Name": "AnalogIO", "640 AO-Sequenceable": "1", "520 Shutter-Demo": "No", "528 Shutter-Demo": "No", "470-Name": "DA Shutter", "520-DA Device": "520 Shutter", "XYStage-Description": "Ludl XY stage driver adapter", "445 Shutter-MinVolts": "0.0000", "528 AO-TriggerSampleRate": "100000", "405 AO-MinVolts": "0.0000", "405 Shutter-TriggerSequenceLength": "1000", "Height": 1200, "555 AO-Block voltage": "false", "640 Shutter-Volts": "0.0000", "PixelType": "GRAY16", "445 Shutter-Block voltage": "false", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "520 Shutter-TriggerSequenceLength": "1000", "COM9-Name": "COM9", "Core-SLM": "", "555 AO-Volts": "0.0000", "555 Shutter-Block voltage": "false", "XYStage-Speed": "1800.0000", "520 AO-MaxVolts": "5.0000", "528 Shutter-SupportsTriggering": "0", "COM1-Description": "Serial port driver (boost:asio)", "PRIME-MetadataEnabled": "No", "520 Shutter-MinVolts": "0.0000", "470 Shutter-Sequenceable": "1", "PRIME-PreampDelay": "0", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-MinVolts": "0.0000", "ChannelIndex": 5, "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Core-AutoShutter": "1", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "Exposure-ms": 300, "PRIME-PP 2 ENABLED": "Yes", "640 Shutter-TriggerSampleRate": "100000", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "470 Shutter-IOChannel": "Dev1/ao10", "405 AO-Demo": "No", "PixelSizeUm": 1.1, "470 AO-Demo": "No", "470 Shutter-MaxVolts": "5.0000", "Vincent-D3-Name": "Vincent-D3", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FileName": "img_000000000_P-DAPI-high_005.tif", "LudlController-Reset": "Operate", "PRIME-Exposure": "300.00", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "445-Name": "DA Shutter", "555 Shutter-Name": "AnalogIO", "520 Shutter-TriggerSampleRate": "100000", "445 Shutter-IOChannel": "Dev1/ao9", "PRIME-TransposeCorrection": "0", "XYStage-StepSize": "0.1000", "445-Description": "DA device that is used as a shutter", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 AO-IOChannel": "Dev1/ao7", "520 Shutter-Name": "AnalogIO", "COM3-DataBits": "8", "640 AO-Volts": "0.0000", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "555 Shutter-IOChannel": "Dev1/ao13", "WaitInterval": null, "520 Shutter-Sequenceable": "1", "YPositionUm": -13012.300000000001, "445-State": "0", "445-DA Device": "445 Shutter", "PRIME-CircularBufferAutoSize": "ON", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "555 AO-TriggerSampleRate": "100000", "445 Shutter-Volts": "0.0000", "XLIGHT Hub-Port": "COM3", "Core-AutoFocus": "", "COM4-Handshaking": "Off", "528 Shutter-MinVolts": "0.0000", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "445 Shutter-Name": "AnalogIO", "528 Shutter-Sequenceable": "1", "XYStage-TransposeMirrorX": "0", "XYStage-TransposeMirrorY": "0", "PRIME-ReadoutRate": "100MHz 16bit", "XYStage-Name": "Stage", "640 AO-SupportsTriggering": "0", "640-Name": "DA Shutter", "640 Shutter-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "640 AO-Block voltage": "false", "445 Shutter-Sequenceable": "1", "470 AO-MaxVolts": "5.0000", "COM4-BaudRate": "9600", "470-Description": "DA device that is used as a shutter", "520 Shutter-Description": "NI DAC", "PRIME-CCDTemperature": "-14.9200", "Time": "2019-12-17 04:00:07 -0500", "640 AO-TriggerSequenceLength": "1000", "470 AO-Name": "AnalogIO", "XLIGHT Disk slider-Label": "Disk Pos 40um", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "Binning": "1x1", "TE2000-ModelType": "1", "COM3-AnswerTimeout": "500.0000", "555 Shutter-SupportsTriggering": "0", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "TE2000-Port": "COM4", "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Description": "NI DAC", "COM4-AnswerTimeout": "500.0000", "COM1-DelayBetweenCharsMs": "0.0000", "FilterBlock-Label": "Confocal", "445 AO-Name": "AnalogIO", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-Timing-ReadoutTimeNs": "24672000.0000", "528 AO-Demo": "No", "520 AO-Sequenceable": "1", "555-Description": "DA device that is used as a shutter", "405-DA Device": "405 Shutter", "405 AO-TriggerSequenceLength": "1000", "640 AO-MinVolts": "0.0000", "640 Shutter-Block voltage": "false", "FrameIndex": 0, "PRIME-Port": "CMOS", "555 AO-MinVolts": "0.0000", "COM3-Description": "Serial port driver (boost:asio)", "528 AO-Description": "NI DAC", "555 AO-IOChannel": "Dev1/ao6", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "555 AO-TriggerInputLine": "/Dev1/PFI0", "PRIME-ActualInterval-ms": "84.6200", "LudlController-Port": "COM9", "445 Shutter-Demo": "No", "PRIME-Trigger-Expose Out-Mux": "1", "SliceIndex": 5, "PRIME-CCDTemperatureSetPoint": "-15.0000", "405 Shutter-MinVolts": "0.0000", "PRIME-ExposeOutMode": "First Row", "AxisPositions": null, "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Volts": "0.0000", "528-Name": "DA Shutter", "555-State": "0", "XLIGHT Dichroic-Label": "QUAD", "445 AO-Sequenceable": "1", "405-Name": "DA Shutter", "405 AO-IOChannel": "Dev1/ao0", "Core-Camera": "PRIME", "Focus-Name": "Focus", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "405 Shutter-IOChannel": "Dev1/ao8", "640 AO-Demo": "No", "640 Shutter-SupportsTriggering": "0", "445 AO-Demo": "No", "555-DA Device": "555 Shutter", "470 Shutter-Description": "NI DAC", "528 AO-Volts": "0.0000", "COM1-DataBits": "8", "Vincent-D3-Address": "x", "520 AO-SupportsTriggering": "0", "405-Description": "DA device that is used as a shutter", "640 Shutter-MinVolts": "0.0000", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "405 AO-Name": "AnalogIO", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "640 AO-TriggerInputLine": "/Dev1/PFI0", "405 AO-TriggerInputLine": "/Dev1/PFI0", "640-Description": "DA device that is used as a shutter", "445 Shutter-TriggerSampleRate": "100000", "470 Shutter-TriggerSequenceLength": "1000", "COM1-StopBits": "1", "520 Shutter-SupportsTriggering": "0", "405-State": "1", "Core-Initialize": "1", "XLIGHT Emission Wheel-State": "3", "520 AO-Description": "NI DAC", "528 AO-TriggerInputLine": "/Dev1/PFI0", "445 Shutter-MaxVolts": "5.0000", "COM9-Handshaking": "Off", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "XLIGHT Spin motor-Label": "On (spinning)", "PRIME-PP 5 ENABLED": "No", "UUID": "602baf6e-3e95-41b2-9a6e-e097b24a41df", "PRIME-TriggerMode": "Internal Trigger", "COM1-Handshaking": "Off", "PRIME-Timing-PostTriggerDelayNs": "49344000.0000", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "Focus-Position": "16732.9000", "528 Shutter-Block voltage": "false", "Channel": "P-DAPI-high", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "555 Shutter-Demo": "No", "405 Shutter-Demo": "No", "528 AO-Name": "AnalogIO", "520-State": "0", "Core-Galvo": "", "405 Shutter-TriggerSampleRate": "100000", "Focus-FocusStepSize_(nm)": "100", "PRIME-PixelType": "16bit", "COM4-DataBits": "8", "470 AO-IOChannel": "Dev1/ao2", "520 AO-MinVolts": "0.0000", "COM9-Parity": "None", "PRIME-CircularBufferEnabled": "ON", "PRIME-PP 6 Reset": "No", "640 AO-Description": "NI DAC", "Source": "PRIME", "445 AO-MinVolts": "0.0000", "PIEZO-Name": "Stage", "445 AO-Description": "NI DAC", "PRIME-CircularBufferFrameCount": "46", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "520 AO-Volts": "0.0000", "528 Shutter-TriggerSampleRate": "100000", "XPositionUm": 23269.100000000002, "OpticalPath-State": "4", "PRIME-PP 3 THRESHOLD": "80", "PRIME-PVCAM Version": "3.7.1", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Gain": "1-HDR", "640 Shutter-TriggerSequenceLength": "1000", "528 AO-SupportsTriggering": "0", "445 AO-TriggerInputLine": "/Dev1/PFI0", "OpticalPath-Name": "OpticalPath", "PRIME-PP 4 ENABLED": "Yes", "PRIME-PVCAM Adapter Version": "1.3.29", "COM3-Name": "COM3", "405 AO-MaxVolts": "5.0000", "520 Shutter-Block voltage": "false", "PRIME-Timing-ClearingTimeNs": "49344000.0000", "Core-Focus": "PIEZO", "528 AO-Block voltage": "false", "TE2000-Description": "Nikon TE2000 hub", "528 AO-MinVolts": "0.0000", "COM4-Parity": "None", "Summary": { "Slices": 11, "ChContrastMin": [ 0, 0, 0, 0, 0, 0 ], "PixelAspect": 1, "SlicesFirst": true, "Interval_ms": 0, "Time": "2019-12-17 03:59:33 -0500", "Prefix": "seq_7", "InitialPositionList": [ { "Label": "Pos0", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [149.91], "Focus": [16657.7], "XYStage": [ 71629.7, 19152.9 ] }, "GridColumnIndex": 0 }, { "Label": "Pos1", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [190.82], "Focus": [16485.4], "XYStage": [ 65702.3, 19396.600000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos2", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [194.69], "Focus": [16446.8], "XYStage": [ 59880.5, 19007.8 ] }, "GridColumnIndex": 0 }, { "Label": "Pos3", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [145.04], "Focus": [16446.6], "XYStage": [ 53616.9, 18818.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos4", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [169.57500000000002], "Focus": [16446.5], "XYStage": [ 47662.8, 18817.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos5", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [131.805], "Focus": [16446.4], "XYStage": [ 41918.8, 18298.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos6", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [113.145], "Focus": [16446.5], "XYStage": [ 36129.9, 18895.8 ] }, "GridColumnIndex": 0 }, { "Label": "Pos7", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [141.005], "Focus": [16446.6], "XYStage": [ 30299.100000000002, 18895.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos8", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [140.97], "Focus": [16446.5], "XYStage": [ 23952.100000000002, 19156 ] }, "GridColumnIndex": 0 }, { "Label": "Pos9", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [184.57500000000002], "Focus": [16677.9], "XYStage": [ 72866.8, -14809.300000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos10", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [167.95000000000002], "Focus": [16557.9], "XYStage": [ 66515.6, -14371.300000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos11", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [168], "Focus": [16618.9], "XYStage": [ 59724.3, -13964.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos12", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [203.205], "Focus": [16779.6], "XYStage": [ 53862.3, -13494.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos13", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [203.20000000000002], "Focus": [16749], "XYStage": [ 48108.3, -13805.400000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos14", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [203.19], "Focus": [16675.9], "XYStage": [ 41645, -13804.900000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos15", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [203.21], "Focus": [16675.7], "XYStage": [ 35352.6, -13805.6 ] }, "GridColumnIndex": 0 }, { "Label": "Pos16", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [203.225], "Focus": [16675.8], "XYStage": [ 29298, -13447.400000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos17", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [203.215], "Focus": [16675.8], "XYStage": [ 23269.100000000002, -13012.300000000001 ] }, "GridColumnIndex": 0 } ], "z-step_um": 10, "GridRow": 0, "BitDepth": 16, "Depth": 2, "Channels": 6, "Source": "Micro-Manager", "ChColors": [ -16711681, -256, -65281, -1, -1, -16776961 ], "MetadataVersion": 10, "KeepShutterOpenChannels": true, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536, 65536 ], "CustomIntervals_ms": [], "Height": 1200, "MicroManagerVersion": "1.4.23 20180219", "GridColumn": 0, "UUID": "3ad8801b-5699-40d6-813b-4ad840863e38", "PixelType": "GRAY16", "Width": 1200, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\Yan\\20191216-YC113-S11S12-miseq", "Frames": 1, "Comment": "", "UserName": "Nikon", "PixelSize_um": 1.1, "ROI": [ 0, 0, 1200, 1200 ], "Date": "2019-12-17", "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC", "P-DAPI-high" ], "KeepShutterOpenSlices": true, "IJType": 1, "ComputerName": "ZadorConfocal2", "Positions": 18, "PositionIndex": 17, "CameraTimeout": 20000, "TimeFirst": false }, "640 AO-Name": "AnalogIO", "Vincent-D3-Command": "Close" }, "FrameKey-0-5-6": { "COM4-StopBits": "1", "528 AO-MaxVolts": "5.0000", "XLIGHT Excitation Wheel-State": "1", "470 Shutter-Name": "AnalogIO", "Core-Shutter": "405", "555 AO-Demo": "No", "XLIGHT Dichroic-State": "2", "XYStage-StepSize-Y": "0.1000", "ZPositionUm": 213.215, "XYStage-StepSize-X": "0.1000", "PRIME-FirmwareVersion": "20.28", "PRIME-ClearCycles": "2", "520-Name": "DA Shutter", "405 Shutter-Name": "AnalogIO", "PIEZO-StepSize": "0.0050", "PRIME-ClearMode": "Pre-Exposure", "470 AO-MinVolts": "0.0000", "405 AO-SupportsTriggering": "1", "CameraChannelIndex": 0, "XYStage-StartSpeed": "500.0000", "COM9-StopBits": "2", "405 AO-Description": "NI DAC", "COM4-DelayBetweenCharsMs": "0.0000", "445 AO-Block voltage": "false", "520 AO-TriggerInputLine": "/Dev1/PFI0", "555 AO-Description": "NI DAC", "520 AO-TriggerSampleRate": "100000", "COM4-Verbose": "1", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM4-Name": "COM4", "405 Shutter-Volts": "5.0000", "Vincent-D3-Port": "COM1", "PRIME-PP 4 MIN ADU AFFECTED": "200", "470 Shutter-MinVolts": "0.0000", "PRIME-PP 3 ENABLED": "Yes", "470 AO-Description": "NI DAC", "PRIME-Description": "PVCAM API device adapter", "520 Shutter-MaxVolts": "5.0000", "520 Shutter-Volts": "0.0000", "528 AO-Sequenceable": "1", "PRIME-Binning": "1x1", "555 Shutter-MaxVolts": "5.0000", "528 Shutter-TriggerSequenceLength": "1000", "405 Shutter-Block voltage": "false", "XLIGHT Spin motor-State": "1", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "Vincent-D3-Time to open (ms)": "35.0000", "Vincent-D3-Time to close (ms)": "35.0000", "445 AO-TriggerSampleRate": "100000", "640 Shutter-IOChannel": "Dev1/ao14", "555 AO-Sequenceable": "1", "520 AO-Name": "AnalogIO", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "470 AO-Block voltage": "false", "COM1-Verbose": "1", "COM9-AnswerTimeout": "10000.0000", "528 Shutter-Name": "AnalogIO", "PRIME-Trigger Timeout (secs)": "10", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "NextFrame": 0, "470 AO-TriggerSequenceLength": "1000", "PRIME-TransposeXY": "0", "445 AO-TriggerSequenceLength": "1000", "COM1-Name": "COM1", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "COM9-DataBits": "8", "COM1-AnswerTimeout": "500.0000", "Camera": "", "TE2000-Version": "V2.50-V2.50-V2.10", "528 Shutter-Volts": "0.0000", "XLIGHT Hub-Name": "XLIGHT Hub", "PIEZO-Description": "Ludl stage driver adapter", "PositionName": "Pos17", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSampleRate": "100000", "PRIME-FanSpeedSetpoint": "High", "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "405 AO-TriggerSampleRate": "100000", "470 Shutter-Block voltage": "false", "470-State": "0", "COM3-Parity": "None", "405 AO-Volts": "4.5000", "PRIME-TransposeMirrorY": "0", "PRIME-ChipName": "GS144BSI", "470 AO-Volts": "0.0000", "PRIME-TransposeMirrorX": "0", "445 Shutter-SupportsTriggering": "0", "470 Shutter-SupportsTriggering": "0", "XLIGHT Hub-Description": "XLIGHT hub", "PRIME-PreampOffLimit": "10000", "470 AO-Sequenceable": "1", "528 Shutter-MaxVolts": "5.0000", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "Core-XYStage": "XYStage", "470 AO-TriggerSampleRate": "100000", "PRIME-Timing-PreTriggerDelayNs": "24672000.0000", "528-DA Device": "528 Shutter", "Focus-Description": "Nikon TE2000 focus adapter", "FilterBlock-State": "5", "520 AO-Demo": "No", "COM3-DelayBetweenCharsMs": "0.0000", "470 Shutter-Volts": "0.0000", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "PRIME-PP 2 THRESHOLD": "125", "PRIME-PP 4 THRESHOLD": "75", "XYStage-Acceleration": "4.0000", "470 Shutter-Demo": "No", "PRIME-Name": "PMPCIECam00", "640-DA Device": "640 Shutter", "405 AO-Block voltage": "false", "ElapsedTime-ms": 751389, "PRIME-Offset": "100", "445 AO-IOChannel": "Dev1/ao1", "640 AO-MaxVolts": "5.0000", "PositionIndex": 17, "PRIME-PP 1 ENABLED": "Yes", "COM1-BaudRate": "9600", "640-State": "0", "COM3-Verbose": "1", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "LudlController-TransmissionDelay": "0", "PRIME-FullWellCapacity": "62000", "PRIME-SMARTStreamingEnabled": "No", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "640 Shutter-Description": "NI DAC", "528-Description": "DA device that is used as a shutter", "COM4-Description": "Serial port driver (boost:asio)", "405 Shutter-Description": "NI DAC", "TE2000-Name": "TE2000", "528 Shutter-Description": "NI DAC", "COM9-Description": "Serial port driver (boost:asio)", "LudlController-Name": "LudlController", "445 Shutter-TriggerSequenceLength": "1000", "PRIME-SerialNumber": "A18B203008", "445 AO-Volts": "0.0000", "PRIME-X-dimension": "1200", "PRIME-ShutterCloseDelay": "0", "XLIGHT Disk slider-State": "2", "Position": "Default", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 AO-IOChannel": "Dev1/ao5", "520 AO-IOChannel": "Dev1/ao4", "528 AO-TriggerSequenceLength": "1000", "405 Shutter-MaxVolts": "5.0000", "470-DA Device": "470 Shutter", "PRIME-FTCapable": "No", "Core-ChannelGroup": "Channels", "555 AO-SupportsTriggering": "0", "PRIME-Y-dimension": "1200", "Frame": 0, "555 Shutter-Sequenceable": "1", "PRIME-Timing-ExposureTimeNs": "499998000.0000", "Core-ImageProcessor": "", "528-State": "0", "520 AO-Block voltage": "false", "XLIGHT Excitation Wheel-Label": "QUAD", "640 Shutter-Demo": "No", "COM3-StopBits": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "520 Shutter-IOChannel": "Dev1/ao11", "Width": 1200, "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-SupportsTriggering": "0", "640 AO-TriggerSampleRate": "100000", "520-Description": "DA device that is used as a shutter", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "405 Shutter-Sequenceable": "1", "405 Shutter-SupportsTriggering": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-TriggerSampleRate": "100000", "640 Shutter-MaxVolts": "5.0000", "555 AO-MaxVolts": "5.0000", "520 AO-TriggerSequenceLength": "1000", "COM3-BaudRate": "9600", "COM9-BaudRate": "9600", "Vincent-D3-Channel #": "Ch. #1", "PRIME-PMode": "Normal", "OpticalPath-Label": "Left(100)", "BitDepth": 16, "555 AO-TriggerSequenceLength": "1000", "445 Shutter-Description": "NI DAC", "405 AO-Sequenceable": "1", "PRIME-AcquisitionMethod": "Callbacks", "555 AO-Name": "AnalogIO", "PRIME-ShutterMode": "Pre-Exposure", "COM3-Handshaking": "Off", "445 AO-SupportsTriggering": "0", "ROI": "0-0-1200-1200", "COM9-Verbose": "1", "PRIME-Color": "OFF", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "PRIME-ShutterOpenDelay": "0", "COM1-Parity": "None", "Slice": 6, "SlicePosition": 10, "COM9-DelayBetweenCharsMs": "0.0000", "555-Name": "DA Shutter", "PIEZO-LudlSingleAxisName": "B", "XLIGHT Emission Wheel-Label": "QUAD", "640 Shutter-Name": "AnalogIO", "640 AO-Sequenceable": "1", "520 Shutter-Demo": "No", "528 Shutter-Demo": "No", "470-Name": "DA Shutter", "520-DA Device": "520 Shutter", "XYStage-Description": "Ludl XY stage driver adapter", "445 Shutter-MinVolts": "0.0000", "528 AO-TriggerSampleRate": "100000", "405 AO-MinVolts": "0.0000", "405 Shutter-TriggerSequenceLength": "1000", "Height": 1200, "555 AO-Block voltage": "false", "640 Shutter-Volts": "0.0000", "PixelType": "GRAY16", "445 Shutter-Block voltage": "false", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "520 Shutter-TriggerSequenceLength": "1000", "COM9-Name": "COM9", "Core-SLM": "", "555 AO-Volts": "0.0000", "555 Shutter-Block voltage": "false", "XYStage-Speed": "1800.0000", "520 AO-MaxVolts": "5.0000", "528 Shutter-SupportsTriggering": "0", "COM1-Description": "Serial port driver (boost:asio)", "PRIME-MetadataEnabled": "No", "520 Shutter-MinVolts": "0.0000", "470 Shutter-Sequenceable": "1", "PRIME-PreampDelay": "0", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-MinVolts": "0.0000", "ChannelIndex": 5, "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Core-AutoShutter": "1", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "Exposure-ms": 300, "PRIME-PP 2 ENABLED": "Yes", "640 Shutter-TriggerSampleRate": "100000", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "470 Shutter-IOChannel": "Dev1/ao10", "405 AO-Demo": "No", "PixelSizeUm": 1.1, "470 AO-Demo": "No", "470 Shutter-MaxVolts": "5.0000", "Vincent-D3-Name": "Vincent-D3", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FileName": "img_000000000_P-DAPI-high_006.tif", "LudlController-Reset": "Operate", "PRIME-Exposure": "300.00", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "445-Name": "DA Shutter", "555 Shutter-Name": "AnalogIO", "520 Shutter-TriggerSampleRate": "100000", "445 Shutter-IOChannel": "Dev1/ao9", "PRIME-TransposeCorrection": "0", "XYStage-StepSize": "0.1000", "445-Description": "DA device that is used as a shutter", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 AO-IOChannel": "Dev1/ao7", "520 Shutter-Name": "AnalogIO", "COM3-DataBits": "8", "640 AO-Volts": "0.0000", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "555 Shutter-IOChannel": "Dev1/ao13", "WaitInterval": null, "520 Shutter-Sequenceable": "1", "YPositionUm": -13012.300000000001, "445-State": "0", "445-DA Device": "445 Shutter", "PRIME-CircularBufferAutoSize": "ON", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "555 AO-TriggerSampleRate": "100000", "445 Shutter-Volts": "0.0000", "XLIGHT Hub-Port": "COM3", "Core-AutoFocus": "", "COM4-Handshaking": "Off", "528 Shutter-MinVolts": "0.0000", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "445 Shutter-Name": "AnalogIO", "528 Shutter-Sequenceable": "1", "XYStage-TransposeMirrorX": "0", "XYStage-TransposeMirrorY": "0", "PRIME-ReadoutRate": "100MHz 16bit", "XYStage-Name": "Stage", "640 AO-SupportsTriggering": "0", "640-Name": "DA Shutter", "640 Shutter-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "640 AO-Block voltage": "false", "445 Shutter-Sequenceable": "1", "470 AO-MaxVolts": "5.0000", "COM4-BaudRate": "9600", "470-Description": "DA device that is used as a shutter", "520 Shutter-Description": "NI DAC", "PRIME-CCDTemperature": "-14.9200", "Time": "2019-12-17 04:00:08 -0500", "640 AO-TriggerSequenceLength": "1000", "470 AO-Name": "AnalogIO", "XLIGHT Disk slider-Label": "Disk Pos 40um", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "Binning": "1x1", "TE2000-ModelType": "1", "COM3-AnswerTimeout": "500.0000", "555 Shutter-SupportsTriggering": "0", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "TE2000-Port": "COM4", "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Description": "NI DAC", "COM4-AnswerTimeout": "500.0000", "COM1-DelayBetweenCharsMs": "0.0000", "FilterBlock-Label": "Confocal", "445 AO-Name": "AnalogIO", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-Timing-ReadoutTimeNs": "24672000.0000", "528 AO-Demo": "No", "520 AO-Sequenceable": "1", "555-Description": "DA device that is used as a shutter", "405-DA Device": "405 Shutter", "405 AO-TriggerSequenceLength": "1000", "640 AO-MinVolts": "0.0000", "640 Shutter-Block voltage": "false", "FrameIndex": 0, "PRIME-Port": "CMOS", "555 AO-MinVolts": "0.0000", "COM3-Description": "Serial port driver (boost:asio)", "528 AO-Description": "NI DAC", "555 AO-IOChannel": "Dev1/ao6", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "555 AO-TriggerInputLine": "/Dev1/PFI0", "PRIME-ActualInterval-ms": "84.6200", "LudlController-Port": "COM9", "445 Shutter-Demo": "No", "PRIME-Trigger-Expose Out-Mux": "1", "SliceIndex": 6, "PRIME-CCDTemperatureSetPoint": "-15.0000", "405 Shutter-MinVolts": "0.0000", "PRIME-ExposeOutMode": "First Row", "AxisPositions": null, "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Volts": "0.0000", "528-Name": "DA Shutter", "555-State": "0", "XLIGHT Dichroic-Label": "QUAD", "445 AO-Sequenceable": "1", "405-Name": "DA Shutter", "405 AO-IOChannel": "Dev1/ao0", "Core-Camera": "PRIME", "Focus-Name": "Focus", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "405 Shutter-IOChannel": "Dev1/ao8", "640 AO-Demo": "No", "640 Shutter-SupportsTriggering": "0", "445 AO-Demo": "No", "555-DA Device": "555 Shutter", "470 Shutter-Description": "NI DAC", "528 AO-Volts": "0.0000", "COM1-DataBits": "8", "Vincent-D3-Address": "x", "520 AO-SupportsTriggering": "0", "405-Description": "DA device that is used as a shutter", "640 Shutter-MinVolts": "0.0000", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "405 AO-Name": "AnalogIO", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "640 AO-TriggerInputLine": "/Dev1/PFI0", "405 AO-TriggerInputLine": "/Dev1/PFI0", "640-Description": "DA device that is used as a shutter", "445 Shutter-TriggerSampleRate": "100000", "470 Shutter-TriggerSequenceLength": "1000", "COM1-StopBits": "1", "520 Shutter-SupportsTriggering": "0", "405-State": "1", "Core-Initialize": "1", "XLIGHT Emission Wheel-State": "3", "520 AO-Description": "NI DAC", "528 AO-TriggerInputLine": "/Dev1/PFI0", "445 Shutter-MaxVolts": "5.0000", "COM9-Handshaking": "Off", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "XLIGHT Spin motor-Label": "On (spinning)", "PRIME-PP 5 ENABLED": "No", "UUID": "18b26bd9-8ae8-44d0-a32e-0decda3b8569", "PRIME-TriggerMode": "Internal Trigger", "COM1-Handshaking": "Off", "PRIME-Timing-PostTriggerDelayNs": "49344000.0000", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "Focus-Position": "16732.9000", "528 Shutter-Block voltage": "false", "Channel": "P-DAPI-high", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "555 Shutter-Demo": "No", "405 Shutter-Demo": "No", "528 AO-Name": "AnalogIO", "520-State": "0", "Core-Galvo": "", "405 Shutter-TriggerSampleRate": "100000", "Focus-FocusStepSize_(nm)": "100", "PRIME-PixelType": "16bit", "COM4-DataBits": "8", "470 AO-IOChannel": "Dev1/ao2", "520 AO-MinVolts": "0.0000", "COM9-Parity": "None", "PRIME-CircularBufferEnabled": "ON", "PRIME-PP 6 Reset": "No", "640 AO-Description": "NI DAC", "Source": "PRIME", "445 AO-MinVolts": "0.0000", "PIEZO-Name": "Stage", "445 AO-Description": "NI DAC", "PRIME-CircularBufferFrameCount": "46", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "520 AO-Volts": "0.0000", "528 Shutter-TriggerSampleRate": "100000", "XPositionUm": 23269.100000000002, "OpticalPath-State": "4", "PRIME-PP 3 THRESHOLD": "80", "PRIME-PVCAM Version": "3.7.1", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Gain": "1-HDR", "640 Shutter-TriggerSequenceLength": "1000", "528 AO-SupportsTriggering": "0", "445 AO-TriggerInputLine": "/Dev1/PFI0", "OpticalPath-Name": "OpticalPath", "PRIME-PP 4 ENABLED": "Yes", "PRIME-PVCAM Adapter Version": "1.3.29", "COM3-Name": "COM3", "405 AO-MaxVolts": "5.0000", "520 Shutter-Block voltage": "false", "PRIME-Timing-ClearingTimeNs": "49344000.0000", "Core-Focus": "PIEZO", "528 AO-Block voltage": "false", "TE2000-Description": "Nikon TE2000 hub", "528 AO-MinVolts": "0.0000", "COM4-Parity": "None", "Summary": { "Slices": 11, "ChContrastMin": [ 0, 0, 0, 0, 0, 0 ], "PixelAspect": 1, "SlicesFirst": true, "Interval_ms": 0, "Time": "2019-12-17 03:59:33 -0500", "Prefix": "seq_7", "InitialPositionList": [ { "Label": "Pos0", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [149.91], "Focus": [16657.7], "XYStage": [ 71629.7, 19152.9 ] }, "GridColumnIndex": 0 }, { "Label": "Pos1", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [190.82], "Focus": [16485.4], "XYStage": [ 65702.3, 19396.600000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos2", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [194.69], "Focus": [16446.8], "XYStage": [ 59880.5, 19007.8 ] }, "GridColumnIndex": 0 }, { "Label": "Pos3", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [145.04], "Focus": [16446.6], "XYStage": [ 53616.9, 18818.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos4", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [169.57500000000002], "Focus": [16446.5], "XYStage": [ 47662.8, 18817.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos5", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [131.805], "Focus": [16446.4], "XYStage": [ 41918.8, 18298.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos6", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [113.145], "Focus": [16446.5], "XYStage": [ 36129.9, 18895.8 ] }, "GridColumnIndex": 0 }, { "Label": "Pos7", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [141.005], "Focus": [16446.6], "XYStage": [ 30299.100000000002, 18895.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos8", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [140.97], "Focus": [16446.5], "XYStage": [ 23952.100000000002, 19156 ] }, "GridColumnIndex": 0 }, { "Label": "Pos9", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [184.57500000000002], "Focus": [16677.9], "XYStage": [ 72866.8, -14809.300000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos10", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [167.95000000000002], "Focus": [16557.9], "XYStage": [ 66515.6, -14371.300000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos11", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [168], "Focus": [16618.9], "XYStage": [ 59724.3, -13964.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos12", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [203.205], "Focus": [16779.6], "XYStage": [ 53862.3, -13494.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos13", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [203.20000000000002], "Focus": [16749], "XYStage": [ 48108.3, -13805.400000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos14", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [203.19], "Focus": [16675.9], "XYStage": [ 41645, -13804.900000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos15", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [203.21], "Focus": [16675.7], "XYStage": [ 35352.6, -13805.6 ] }, "GridColumnIndex": 0 }, { "Label": "Pos16", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [203.225], "Focus": [16675.8], "XYStage": [ 29298, -13447.400000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos17", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [203.215], "Focus": [16675.8], "XYStage": [ 23269.100000000002, -13012.300000000001 ] }, "GridColumnIndex": 0 } ], "z-step_um": 10, "GridRow": 0, "BitDepth": 16, "Depth": 2, "Channels": 6, "Source": "Micro-Manager", "ChColors": [ -16711681, -256, -65281, -1, -1, -16776961 ], "MetadataVersion": 10, "KeepShutterOpenChannels": true, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536, 65536 ], "CustomIntervals_ms": [], "Height": 1200, "MicroManagerVersion": "1.4.23 20180219", "GridColumn": 0, "UUID": "3ad8801b-5699-40d6-813b-4ad840863e38", "PixelType": "GRAY16", "Width": 1200, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\Yan\\20191216-YC113-S11S12-miseq", "Frames": 1, "Comment": "", "UserName": "Nikon", "PixelSize_um": 1.1, "ROI": [ 0, 0, 1200, 1200 ], "Date": "2019-12-17", "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC", "P-DAPI-high" ], "KeepShutterOpenSlices": true, "IJType": 1, "ComputerName": "ZadorConfocal2", "Positions": 18, "PositionIndex": 17, "CameraTimeout": 20000, "TimeFirst": false }, "640 AO-Name": "AnalogIO", "Vincent-D3-Command": "Close" }, "FrameKey-0-5-7": { "COM4-StopBits": "1", "528 AO-MaxVolts": "5.0000", "XLIGHT Excitation Wheel-State": "1", "470 Shutter-Name": "AnalogIO", "Core-Shutter": "405", "555 AO-Demo": "No", "XLIGHT Dichroic-State": "2", "XYStage-StepSize-Y": "0.1000", "ZPositionUm": 223.215, "XYStage-StepSize-X": "0.1000", "PRIME-FirmwareVersion": "20.28", "PRIME-ClearCycles": "2", "520-Name": "DA Shutter", "405 Shutter-Name": "AnalogIO", "PIEZO-StepSize": "0.0050", "PRIME-ClearMode": "Pre-Exposure", "470 AO-MinVolts": "0.0000", "405 AO-SupportsTriggering": "1", "CameraChannelIndex": 0, "XYStage-StartSpeed": "500.0000", "COM9-StopBits": "2", "405 AO-Description": "NI DAC", "COM4-DelayBetweenCharsMs": "0.0000", "445 AO-Block voltage": "false", "520 AO-TriggerInputLine": "/Dev1/PFI0", "555 AO-Description": "NI DAC", "520 AO-TriggerSampleRate": "100000", "COM4-Verbose": "1", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM4-Name": "COM4", "405 Shutter-Volts": "5.0000", "Vincent-D3-Port": "COM1", "PRIME-PP 4 MIN ADU AFFECTED": "200", "470 Shutter-MinVolts": "0.0000", "PRIME-PP 3 ENABLED": "Yes", "470 AO-Description": "NI DAC", "PRIME-Description": "PVCAM API device adapter", "520 Shutter-MaxVolts": "5.0000", "520 Shutter-Volts": "0.0000", "528 AO-Sequenceable": "1", "PRIME-Binning": "1x1", "555 Shutter-MaxVolts": "5.0000", "528 Shutter-TriggerSequenceLength": "1000", "405 Shutter-Block voltage": "false", "XLIGHT Spin motor-State": "1", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "Vincent-D3-Time to open (ms)": "35.0000", "Vincent-D3-Time to close (ms)": "35.0000", "445 AO-TriggerSampleRate": "100000", "640 Shutter-IOChannel": "Dev1/ao14", "555 AO-Sequenceable": "1", "520 AO-Name": "AnalogIO", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "470 AO-Block voltage": "false", "COM1-Verbose": "1", "COM9-AnswerTimeout": "10000.0000", "528 Shutter-Name": "AnalogIO", "PRIME-Trigger Timeout (secs)": "10", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "NextFrame": 0, "470 AO-TriggerSequenceLength": "1000", "PRIME-TransposeXY": "0", "445 AO-TriggerSequenceLength": "1000", "COM1-Name": "COM1", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "COM9-DataBits": "8", "COM1-AnswerTimeout": "500.0000", "Camera": "", "TE2000-Version": "V2.50-V2.50-V2.10", "528 Shutter-Volts": "0.0000", "XLIGHT Hub-Name": "XLIGHT Hub", "PIEZO-Description": "Ludl stage driver adapter", "PositionName": "Pos17", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSampleRate": "100000", "PRIME-FanSpeedSetpoint": "High", "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "405 AO-TriggerSampleRate": "100000", "470 Shutter-Block voltage": "false", "470-State": "0", "COM3-Parity": "None", "405 AO-Volts": "4.5000", "PRIME-TransposeMirrorY": "0", "PRIME-ChipName": "GS144BSI", "470 AO-Volts": "0.0000", "PRIME-TransposeMirrorX": "0", "445 Shutter-SupportsTriggering": "0", "470 Shutter-SupportsTriggering": "0", "XLIGHT Hub-Description": "XLIGHT hub", "PRIME-PreampOffLimit": "10000", "470 AO-Sequenceable": "1", "528 Shutter-MaxVolts": "5.0000", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "Core-XYStage": "XYStage", "470 AO-TriggerSampleRate": "100000", "PRIME-Timing-PreTriggerDelayNs": "24672000.0000", "528-DA Device": "528 Shutter", "Focus-Description": "Nikon TE2000 focus adapter", "FilterBlock-State": "5", "520 AO-Demo": "No", "COM3-DelayBetweenCharsMs": "0.0000", "470 Shutter-Volts": "0.0000", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "PRIME-PP 2 THRESHOLD": "125", "PRIME-PP 4 THRESHOLD": "75", "XYStage-Acceleration": "4.0000", "470 Shutter-Demo": "No", "PRIME-Name": "PMPCIECam00", "640-DA Device": "640 Shutter", "405 AO-Block voltage": "false", "ElapsedTime-ms": 751932, "PRIME-Offset": "100", "445 AO-IOChannel": "Dev1/ao1", "640 AO-MaxVolts": "5.0000", "PositionIndex": 17, "PRIME-PP 1 ENABLED": "Yes", "COM1-BaudRate": "9600", "640-State": "0", "COM3-Verbose": "1", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "LudlController-TransmissionDelay": "0", "PRIME-FullWellCapacity": "62000", "PRIME-SMARTStreamingEnabled": "No", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "640 Shutter-Description": "NI DAC", "528-Description": "DA device that is used as a shutter", "COM4-Description": "Serial port driver (boost:asio)", "405 Shutter-Description": "NI DAC", "TE2000-Name": "TE2000", "528 Shutter-Description": "NI DAC", "COM9-Description": "Serial port driver (boost:asio)", "LudlController-Name": "LudlController", "445 Shutter-TriggerSequenceLength": "1000", "PRIME-SerialNumber": "A18B203008", "445 AO-Volts": "0.0000", "PRIME-X-dimension": "1200", "PRIME-ShutterCloseDelay": "0", "XLIGHT Disk slider-State": "2", "Position": "Default", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 AO-IOChannel": "Dev1/ao5", "520 AO-IOChannel": "Dev1/ao4", "528 AO-TriggerSequenceLength": "1000", "405 Shutter-MaxVolts": "5.0000", "470-DA Device": "470 Shutter", "PRIME-FTCapable": "No", "Core-ChannelGroup": "Channels", "555 AO-SupportsTriggering": "0", "PRIME-Y-dimension": "1200", "Frame": 0, "555 Shutter-Sequenceable": "1", "PRIME-Timing-ExposureTimeNs": "499998000.0000", "Core-ImageProcessor": "", "528-State": "0", "520 AO-Block voltage": "false", "XLIGHT Excitation Wheel-Label": "QUAD", "640 Shutter-Demo": "No", "COM3-StopBits": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "520 Shutter-IOChannel": "Dev1/ao11", "Width": 1200, "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-SupportsTriggering": "0", "640 AO-TriggerSampleRate": "100000", "520-Description": "DA device that is used as a shutter", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "405 Shutter-Sequenceable": "1", "405 Shutter-SupportsTriggering": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-TriggerSampleRate": "100000", "640 Shutter-MaxVolts": "5.0000", "555 AO-MaxVolts": "5.0000", "520 AO-TriggerSequenceLength": "1000", "COM3-BaudRate": "9600", "COM9-BaudRate": "9600", "Vincent-D3-Channel #": "Ch. #1", "PRIME-PMode": "Normal", "OpticalPath-Label": "Left(100)", "BitDepth": 16, "555 AO-TriggerSequenceLength": "1000", "445 Shutter-Description": "NI DAC", "405 AO-Sequenceable": "1", "PRIME-AcquisitionMethod": "Callbacks", "555 AO-Name": "AnalogIO", "PRIME-ShutterMode": "Pre-Exposure", "COM3-Handshaking": "Off", "445 AO-SupportsTriggering": "0", "ROI": "0-0-1200-1200", "COM9-Verbose": "1", "PRIME-Color": "OFF", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "PRIME-ShutterOpenDelay": "0", "COM1-Parity": "None", "Slice": 7, "SlicePosition": 20, "COM9-DelayBetweenCharsMs": "0.0000", "555-Name": "DA Shutter", "PIEZO-LudlSingleAxisName": "B", "XLIGHT Emission Wheel-Label": "QUAD", "640 Shutter-Name": "AnalogIO", "640 AO-Sequenceable": "1", "520 Shutter-Demo": "No", "528 Shutter-Demo": "No", "470-Name": "DA Shutter", "520-DA Device": "520 Shutter", "XYStage-Description": "Ludl XY stage driver adapter", "445 Shutter-MinVolts": "0.0000", "528 AO-TriggerSampleRate": "100000", "405 AO-MinVolts": "0.0000", "405 Shutter-TriggerSequenceLength": "1000", "Height": 1200, "555 AO-Block voltage": "false", "640 Shutter-Volts": "0.0000", "PixelType": "GRAY16", "445 Shutter-Block voltage": "false", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "520 Shutter-TriggerSequenceLength": "1000", "COM9-Name": "COM9", "Core-SLM": "", "555 AO-Volts": "0.0000", "555 Shutter-Block voltage": "false", "XYStage-Speed": "1800.0000", "520 AO-MaxVolts": "5.0000", "528 Shutter-SupportsTriggering": "0", "COM1-Description": "Serial port driver (boost:asio)", "PRIME-MetadataEnabled": "No", "520 Shutter-MinVolts": "0.0000", "470 Shutter-Sequenceable": "1", "PRIME-PreampDelay": "0", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-MinVolts": "0.0000", "ChannelIndex": 5, "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Core-AutoShutter": "1", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "Exposure-ms": 300, "PRIME-PP 2 ENABLED": "Yes", "640 Shutter-TriggerSampleRate": "100000", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "470 Shutter-IOChannel": "Dev1/ao10", "405 AO-Demo": "No", "PixelSizeUm": 1.1, "470 AO-Demo": "No", "470 Shutter-MaxVolts": "5.0000", "Vincent-D3-Name": "Vincent-D3", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FileName": "img_000000000_P-DAPI-high_007.tif", "LudlController-Reset": "Operate", "PRIME-Exposure": "300.00", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "445-Name": "DA Shutter", "555 Shutter-Name": "AnalogIO", "520 Shutter-TriggerSampleRate": "100000", "445 Shutter-IOChannel": "Dev1/ao9", "PRIME-TransposeCorrection": "0", "XYStage-StepSize": "0.1000", "445-Description": "DA device that is used as a shutter", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 AO-IOChannel": "Dev1/ao7", "520 Shutter-Name": "AnalogIO", "COM3-DataBits": "8", "640 AO-Volts": "0.0000", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "555 Shutter-IOChannel": "Dev1/ao13", "WaitInterval": null, "520 Shutter-Sequenceable": "1", "YPositionUm": -13012.300000000001, "445-State": "0", "445-DA Device": "445 Shutter", "PRIME-CircularBufferAutoSize": "ON", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "555 AO-TriggerSampleRate": "100000", "445 Shutter-Volts": "0.0000", "XLIGHT Hub-Port": "COM3", "Core-AutoFocus": "", "COM4-Handshaking": "Off", "528 Shutter-MinVolts": "0.0000", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "445 Shutter-Name": "AnalogIO", "528 Shutter-Sequenceable": "1", "XYStage-TransposeMirrorX": "0", "XYStage-TransposeMirrorY": "0", "PRIME-ReadoutRate": "100MHz 16bit", "XYStage-Name": "Stage", "640 AO-SupportsTriggering": "0", "640-Name": "DA Shutter", "640 Shutter-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "640 AO-Block voltage": "false", "445 Shutter-Sequenceable": "1", "470 AO-MaxVolts": "5.0000", "COM4-BaudRate": "9600", "470-Description": "DA device that is used as a shutter", "520 Shutter-Description": "NI DAC", "PRIME-CCDTemperature": "-14.9200", "Time": "2019-12-17 04:00:08 -0500", "640 AO-TriggerSequenceLength": "1000", "470 AO-Name": "AnalogIO", "XLIGHT Disk slider-Label": "Disk Pos 40um", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "Binning": "1x1", "TE2000-ModelType": "1", "COM3-AnswerTimeout": "500.0000", "555 Shutter-SupportsTriggering": "0", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "TE2000-Port": "COM4", "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Description": "NI DAC", "COM4-AnswerTimeout": "500.0000", "COM1-DelayBetweenCharsMs": "0.0000", "FilterBlock-Label": "Confocal", "445 AO-Name": "AnalogIO", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-Timing-ReadoutTimeNs": "24672000.0000", "528 AO-Demo": "No", "520 AO-Sequenceable": "1", "555-Description": "DA device that is used as a shutter", "405-DA Device": "405 Shutter", "405 AO-TriggerSequenceLength": "1000", "640 AO-MinVolts": "0.0000", "640 Shutter-Block voltage": "false", "FrameIndex": 0, "PRIME-Port": "CMOS", "555 AO-MinVolts": "0.0000", "COM3-Description": "Serial port driver (boost:asio)", "528 AO-Description": "NI DAC", "555 AO-IOChannel": "Dev1/ao6", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "555 AO-TriggerInputLine": "/Dev1/PFI0", "PRIME-ActualInterval-ms": "84.6200", "LudlController-Port": "COM9", "445 Shutter-Demo": "No", "PRIME-Trigger-Expose Out-Mux": "1", "SliceIndex": 7, "PRIME-CCDTemperatureSetPoint": "-15.0000", "405 Shutter-MinVolts": "0.0000", "PRIME-ExposeOutMode": "First Row", "AxisPositions": null, "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Volts": "0.0000", "528-Name": "DA Shutter", "555-State": "0", "XLIGHT Dichroic-Label": "QUAD", "445 AO-Sequenceable": "1", "405-Name": "DA Shutter", "405 AO-IOChannel": "Dev1/ao0", "Core-Camera": "PRIME", "Focus-Name": "Focus", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "405 Shutter-IOChannel": "Dev1/ao8", "640 AO-Demo": "No", "640 Shutter-SupportsTriggering": "0", "445 AO-Demo": "No", "555-DA Device": "555 Shutter", "470 Shutter-Description": "NI DAC", "528 AO-Volts": "0.0000", "COM1-DataBits": "8", "Vincent-D3-Address": "x", "520 AO-SupportsTriggering": "0", "405-Description": "DA device that is used as a shutter", "640 Shutter-MinVolts": "0.0000", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "405 AO-Name": "AnalogIO", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "640 AO-TriggerInputLine": "/Dev1/PFI0", "405 AO-TriggerInputLine": "/Dev1/PFI0", "640-Description": "DA device that is used as a shutter", "445 Shutter-TriggerSampleRate": "100000", "470 Shutter-TriggerSequenceLength": "1000", "COM1-StopBits": "1", "520 Shutter-SupportsTriggering": "0", "405-State": "1", "Core-Initialize": "1", "XLIGHT Emission Wheel-State": "3", "520 AO-Description": "NI DAC", "528 AO-TriggerInputLine": "/Dev1/PFI0", "445 Shutter-MaxVolts": "5.0000", "COM9-Handshaking": "Off", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "XLIGHT Spin motor-Label": "On (spinning)", "PRIME-PP 5 ENABLED": "No", "UUID": "db9de72d-0e8e-48c5-8fe9-54e20e919e25", "PRIME-TriggerMode": "Internal Trigger", "COM1-Handshaking": "Off", "PRIME-Timing-PostTriggerDelayNs": "49344000.0000", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "Focus-Position": "16732.9000", "528 Shutter-Block voltage": "false", "Channel": "P-DAPI-high", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "555 Shutter-Demo": "No", "405 Shutter-Demo": "No", "528 AO-Name": "AnalogIO", "520-State": "0", "Core-Galvo": "", "405 Shutter-TriggerSampleRate": "100000", "Focus-FocusStepSize_(nm)": "100", "PRIME-PixelType": "16bit", "COM4-DataBits": "8", "470 AO-IOChannel": "Dev1/ao2", "520 AO-MinVolts": "0.0000", "COM9-Parity": "None", "PRIME-CircularBufferEnabled": "ON", "PRIME-PP 6 Reset": "No", "640 AO-Description": "NI DAC", "Source": "PRIME", "445 AO-MinVolts": "0.0000", "PIEZO-Name": "Stage", "445 AO-Description": "NI DAC", "PRIME-CircularBufferFrameCount": "46", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "520 AO-Volts": "0.0000", "528 Shutter-TriggerSampleRate": "100000", "XPositionUm": 23269.100000000002, "OpticalPath-State": "4", "PRIME-PP 3 THRESHOLD": "80", "PRIME-PVCAM Version": "3.7.1", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Gain": "1-HDR", "640 Shutter-TriggerSequenceLength": "1000", "528 AO-SupportsTriggering": "0", "445 AO-TriggerInputLine": "/Dev1/PFI0", "OpticalPath-Name": "OpticalPath", "PRIME-PP 4 ENABLED": "Yes", "PRIME-PVCAM Adapter Version": "1.3.29", "COM3-Name": "COM3", "405 AO-MaxVolts": "5.0000", "520 Shutter-Block voltage": "false", "PRIME-Timing-ClearingTimeNs": "49344000.0000", "Core-Focus": "PIEZO", "528 AO-Block voltage": "false", "TE2000-Description": "Nikon TE2000 hub", "528 AO-MinVolts": "0.0000", "COM4-Parity": "None", "Summary": { "Slices": 11, "ChContrastMin": [ 0, 0, 0, 0, 0, 0 ], "PixelAspect": 1, "SlicesFirst": true, "Interval_ms": 0, "Time": "2019-12-17 03:59:33 -0500", "Prefix": "seq_7", "InitialPositionList": [ { "Label": "Pos0", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [149.91], "Focus": [16657.7], "XYStage": [ 71629.7, 19152.9 ] }, "GridColumnIndex": 0 }, { "Label": "Pos1", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [190.82], "Focus": [16485.4], "XYStage": [ 65702.3, 19396.600000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos2", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [194.69], "Focus": [16446.8], "XYStage": [ 59880.5, 19007.8 ] }, "GridColumnIndex": 0 }, { "Label": "Pos3", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [145.04], "Focus": [16446.6], "XYStage": [ 53616.9, 18818.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos4", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [169.57500000000002], "Focus": [16446.5], "XYStage": [ 47662.8, 18817.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos5", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [131.805], "Focus": [16446.4], "XYStage": [ 41918.8, 18298.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos6", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [113.145], "Focus": [16446.5], "XYStage": [ 36129.9, 18895.8 ] }, "GridColumnIndex": 0 }, { "Label": "Pos7", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [141.005], "Focus": [16446.6], "XYStage": [ 30299.100000000002, 18895.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos8", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [140.97], "Focus": [16446.5], "XYStage": [ 23952.100000000002, 19156 ] }, "GridColumnIndex": 0 }, { "Label": "Pos9", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [184.57500000000002], "Focus": [16677.9], "XYStage": [ 72866.8, -14809.300000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos10", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [167.95000000000002], "Focus": [16557.9], "XYStage": [ 66515.6, -14371.300000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos11", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [168], "Focus": [16618.9], "XYStage": [ 59724.3, -13964.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos12", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [203.205], "Focus": [16779.6], "XYStage": [ 53862.3, -13494.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos13", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [203.20000000000002], "Focus": [16749], "XYStage": [ 48108.3, -13805.400000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos14", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [203.19], "Focus": [16675.9], "XYStage": [ 41645, -13804.900000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos15", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [203.21], "Focus": [16675.7], "XYStage": [ 35352.6, -13805.6 ] }, "GridColumnIndex": 0 }, { "Label": "Pos16", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [203.225], "Focus": [16675.8], "XYStage": [ 29298, -13447.400000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos17", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [203.215], "Focus": [16675.8], "XYStage": [ 23269.100000000002, -13012.300000000001 ] }, "GridColumnIndex": 0 } ], "z-step_um": 10, "GridRow": 0, "BitDepth": 16, "Depth": 2, "Channels": 6, "Source": "Micro-Manager", "ChColors": [ -16711681, -256, -65281, -1, -1, -16776961 ], "MetadataVersion": 10, "KeepShutterOpenChannels": true, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536, 65536 ], "CustomIntervals_ms": [], "Height": 1200, "MicroManagerVersion": "1.4.23 20180219", "GridColumn": 0, "UUID": "3ad8801b-5699-40d6-813b-4ad840863e38", "PixelType": "GRAY16", "Width": 1200, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\Yan\\20191216-YC113-S11S12-miseq", "Frames": 1, "Comment": "", "UserName": "Nikon", "PixelSize_um": 1.1, "ROI": [ 0, 0, 1200, 1200 ], "Date": "2019-12-17", "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC", "P-DAPI-high" ], "KeepShutterOpenSlices": true, "IJType": 1, "ComputerName": "ZadorConfocal2", "Positions": 18, "PositionIndex": 17, "CameraTimeout": 20000, "TimeFirst": false }, "640 AO-Name": "AnalogIO", "Vincent-D3-Command": "Close" }, "FrameKey-0-5-8": { "COM4-StopBits": "1", "528 AO-MaxVolts": "5.0000", "XLIGHT Excitation Wheel-State": "1", "470 Shutter-Name": "AnalogIO", "Core-Shutter": "405", "555 AO-Demo": "No", "XLIGHT Dichroic-State": "2", "XYStage-StepSize-Y": "0.1000", "ZPositionUm": 233.215, "XYStage-StepSize-X": "0.1000", "PRIME-FirmwareVersion": "20.28", "PRIME-ClearCycles": "2", "520-Name": "DA Shutter", "405 Shutter-Name": "AnalogIO", "PIEZO-StepSize": "0.0050", "PRIME-ClearMode": "Pre-Exposure", "470 AO-MinVolts": "0.0000", "405 AO-SupportsTriggering": "1", "CameraChannelIndex": 0, "XYStage-StartSpeed": "500.0000", "COM9-StopBits": "2", "405 AO-Description": "NI DAC", "COM4-DelayBetweenCharsMs": "0.0000", "445 AO-Block voltage": "false", "520 AO-TriggerInputLine": "/Dev1/PFI0", "555 AO-Description": "NI DAC", "520 AO-TriggerSampleRate": "100000", "COM4-Verbose": "1", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM4-Name": "COM4", "405 Shutter-Volts": "5.0000", "Vincent-D3-Port": "COM1", "PRIME-PP 4 MIN ADU AFFECTED": "200", "470 Shutter-MinVolts": "0.0000", "PRIME-PP 3 ENABLED": "Yes", "470 AO-Description": "NI DAC", "PRIME-Description": "PVCAM API device adapter", "520 Shutter-MaxVolts": "5.0000", "520 Shutter-Volts": "0.0000", "528 AO-Sequenceable": "1", "PRIME-Binning": "1x1", "555 Shutter-MaxVolts": "5.0000", "528 Shutter-TriggerSequenceLength": "1000", "405 Shutter-Block voltage": "false", "XLIGHT Spin motor-State": "1", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "Vincent-D3-Time to open (ms)": "35.0000", "Vincent-D3-Time to close (ms)": "35.0000", "445 AO-TriggerSampleRate": "100000", "640 Shutter-IOChannel": "Dev1/ao14", "555 AO-Sequenceable": "1", "520 AO-Name": "AnalogIO", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "470 AO-Block voltage": "false", "COM1-Verbose": "1", "COM9-AnswerTimeout": "10000.0000", "528 Shutter-Name": "AnalogIO", "PRIME-Trigger Timeout (secs)": "10", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "NextFrame": 0, "470 AO-TriggerSequenceLength": "1000", "PRIME-TransposeXY": "0", "445 AO-TriggerSequenceLength": "1000", "COM1-Name": "COM1", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "COM9-DataBits": "8", "COM1-AnswerTimeout": "500.0000", "Camera": "", "TE2000-Version": "V2.50-V2.50-V2.10", "528 Shutter-Volts": "0.0000", "XLIGHT Hub-Name": "XLIGHT Hub", "PIEZO-Description": "Ludl stage driver adapter", "PositionName": "Pos17", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSampleRate": "100000", "PRIME-FanSpeedSetpoint": "High", "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "405 AO-TriggerSampleRate": "100000", "470 Shutter-Block voltage": "false", "470-State": "0", "COM3-Parity": "None", "405 AO-Volts": "4.5000", "PRIME-TransposeMirrorY": "0", "PRIME-ChipName": "GS144BSI", "470 AO-Volts": "0.0000", "PRIME-TransposeMirrorX": "0", "445 Shutter-SupportsTriggering": "0", "470 Shutter-SupportsTriggering": "0", "XLIGHT Hub-Description": "XLIGHT hub", "PRIME-PreampOffLimit": "10000", "470 AO-Sequenceable": "1", "528 Shutter-MaxVolts": "5.0000", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "Core-XYStage": "XYStage", "470 AO-TriggerSampleRate": "100000", "PRIME-Timing-PreTriggerDelayNs": "24672000.0000", "528-DA Device": "528 Shutter", "Focus-Description": "Nikon TE2000 focus adapter", "FilterBlock-State": "5", "520 AO-Demo": "No", "COM3-DelayBetweenCharsMs": "0.0000", "470 Shutter-Volts": "0.0000", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "PRIME-PP 2 THRESHOLD": "125", "PRIME-PP 4 THRESHOLD": "75", "XYStage-Acceleration": "4.0000", "470 Shutter-Demo": "No", "PRIME-Name": "PMPCIECam00", "640-DA Device": "640 Shutter", "405 AO-Block voltage": "false", "ElapsedTime-ms": 752476, "PRIME-Offset": "100", "445 AO-IOChannel": "Dev1/ao1", "640 AO-MaxVolts": "5.0000", "PositionIndex": 17, "PRIME-PP 1 ENABLED": "Yes", "COM1-BaudRate": "9600", "640-State": "0", "COM3-Verbose": "1", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "LudlController-TransmissionDelay": "0", "PRIME-FullWellCapacity": "62000", "PRIME-SMARTStreamingEnabled": "No", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "640 Shutter-Description": "NI DAC", "528-Description": "DA device that is used as a shutter", "COM4-Description": "Serial port driver (boost:asio)", "405 Shutter-Description": "NI DAC", "TE2000-Name": "TE2000", "528 Shutter-Description": "NI DAC", "COM9-Description": "Serial port driver (boost:asio)", "LudlController-Name": "LudlController", "445 Shutter-TriggerSequenceLength": "1000", "PRIME-SerialNumber": "A18B203008", "445 AO-Volts": "0.0000", "PRIME-X-dimension": "1200", "PRIME-ShutterCloseDelay": "0", "XLIGHT Disk slider-State": "2", "Position": "Default", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 AO-IOChannel": "Dev1/ao5", "520 AO-IOChannel": "Dev1/ao4", "528 AO-TriggerSequenceLength": "1000", "405 Shutter-MaxVolts": "5.0000", "470-DA Device": "470 Shutter", "PRIME-FTCapable": "No", "Core-ChannelGroup": "Channels", "555 AO-SupportsTriggering": "0", "PRIME-Y-dimension": "1200", "Frame": 0, "555 Shutter-Sequenceable": "1", "PRIME-Timing-ExposureTimeNs": "499998000.0000", "Core-ImageProcessor": "", "528-State": "0", "520 AO-Block voltage": "false", "XLIGHT Excitation Wheel-Label": "QUAD", "640 Shutter-Demo": "No", "COM3-StopBits": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "520 Shutter-IOChannel": "Dev1/ao11", "Width": 1200, "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-SupportsTriggering": "0", "640 AO-TriggerSampleRate": "100000", "520-Description": "DA device that is used as a shutter", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "405 Shutter-Sequenceable": "1", "405 Shutter-SupportsTriggering": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-TriggerSampleRate": "100000", "640 Shutter-MaxVolts": "5.0000", "555 AO-MaxVolts": "5.0000", "520 AO-TriggerSequenceLength": "1000", "COM3-BaudRate": "9600", "COM9-BaudRate": "9600", "Vincent-D3-Channel #": "Ch. #1", "PRIME-PMode": "Normal", "OpticalPath-Label": "Left(100)", "BitDepth": 16, "555 AO-TriggerSequenceLength": "1000", "445 Shutter-Description": "NI DAC", "405 AO-Sequenceable": "1", "PRIME-AcquisitionMethod": "Callbacks", "555 AO-Name": "AnalogIO", "PRIME-ShutterMode": "Pre-Exposure", "COM3-Handshaking": "Off", "445 AO-SupportsTriggering": "0", "ROI": "0-0-1200-1200", "COM9-Verbose": "1", "PRIME-Color": "OFF", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "PRIME-ShutterOpenDelay": "0", "COM1-Parity": "None", "Slice": 8, "SlicePosition": 30, "COM9-DelayBetweenCharsMs": "0.0000", "555-Name": "DA Shutter", "PIEZO-LudlSingleAxisName": "B", "XLIGHT Emission Wheel-Label": "QUAD", "640 Shutter-Name": "AnalogIO", "640 AO-Sequenceable": "1", "520 Shutter-Demo": "No", "528 Shutter-Demo": "No", "470-Name": "DA Shutter", "520-DA Device": "520 Shutter", "XYStage-Description": "Ludl XY stage driver adapter", "445 Shutter-MinVolts": "0.0000", "528 AO-TriggerSampleRate": "100000", "405 AO-MinVolts": "0.0000", "405 Shutter-TriggerSequenceLength": "1000", "Height": 1200, "555 AO-Block voltage": "false", "640 Shutter-Volts": "0.0000", "PixelType": "GRAY16", "445 Shutter-Block voltage": "false", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "520 Shutter-TriggerSequenceLength": "1000", "COM9-Name": "COM9", "Core-SLM": "", "555 AO-Volts": "0.0000", "555 Shutter-Block voltage": "false", "XYStage-Speed": "1800.0000", "520 AO-MaxVolts": "5.0000", "528 Shutter-SupportsTriggering": "0", "COM1-Description": "Serial port driver (boost:asio)", "PRIME-MetadataEnabled": "No", "520 Shutter-MinVolts": "0.0000", "470 Shutter-Sequenceable": "1", "PRIME-PreampDelay": "0", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-MinVolts": "0.0000", "ChannelIndex": 5, "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Core-AutoShutter": "1", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "Exposure-ms": 300, "PRIME-PP 2 ENABLED": "Yes", "640 Shutter-TriggerSampleRate": "100000", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "470 Shutter-IOChannel": "Dev1/ao10", "405 AO-Demo": "No", "PixelSizeUm": 1.1, "470 AO-Demo": "No", "470 Shutter-MaxVolts": "5.0000", "Vincent-D3-Name": "Vincent-D3", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FileName": "img_000000000_P-DAPI-high_008.tif", "LudlController-Reset": "Operate", "PRIME-Exposure": "300.00", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "445-Name": "DA Shutter", "555 Shutter-Name": "AnalogIO", "520 Shutter-TriggerSampleRate": "100000", "445 Shutter-IOChannel": "Dev1/ao9", "PRIME-TransposeCorrection": "0", "XYStage-StepSize": "0.1000", "445-Description": "DA device that is used as a shutter", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 AO-IOChannel": "Dev1/ao7", "520 Shutter-Name": "AnalogIO", "COM3-DataBits": "8", "640 AO-Volts": "0.0000", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "555 Shutter-IOChannel": "Dev1/ao13", "WaitInterval": null, "520 Shutter-Sequenceable": "1", "YPositionUm": -13012.300000000001, "445-State": "0", "445-DA Device": "445 Shutter", "PRIME-CircularBufferAutoSize": "ON", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "555 AO-TriggerSampleRate": "100000", "445 Shutter-Volts": "0.0000", "XLIGHT Hub-Port": "COM3", "Core-AutoFocus": "", "COM4-Handshaking": "Off", "528 Shutter-MinVolts": "0.0000", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "445 Shutter-Name": "AnalogIO", "528 Shutter-Sequenceable": "1", "XYStage-TransposeMirrorX": "0", "XYStage-TransposeMirrorY": "0", "PRIME-ReadoutRate": "100MHz 16bit", "XYStage-Name": "Stage", "640 AO-SupportsTriggering": "0", "640-Name": "DA Shutter", "640 Shutter-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "640 AO-Block voltage": "false", "445 Shutter-Sequenceable": "1", "470 AO-MaxVolts": "5.0000", "COM4-BaudRate": "9600", "470-Description": "DA device that is used as a shutter", "520 Shutter-Description": "NI DAC", "PRIME-CCDTemperature": "-14.9200", "Time": "2019-12-17 04:00:09 -0500", "640 AO-TriggerSequenceLength": "1000", "470 AO-Name": "AnalogIO", "XLIGHT Disk slider-Label": "Disk Pos 40um", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "Binning": "1x1", "TE2000-ModelType": "1", "COM3-AnswerTimeout": "500.0000", "555 Shutter-SupportsTriggering": "0", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "TE2000-Port": "COM4", "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Description": "NI DAC", "COM4-AnswerTimeout": "500.0000", "COM1-DelayBetweenCharsMs": "0.0000", "FilterBlock-Label": "Confocal", "445 AO-Name": "AnalogIO", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-Timing-ReadoutTimeNs": "24672000.0000", "528 AO-Demo": "No", "520 AO-Sequenceable": "1", "555-Description": "DA device that is used as a shutter", "405-DA Device": "405 Shutter", "405 AO-TriggerSequenceLength": "1000", "640 AO-MinVolts": "0.0000", "640 Shutter-Block voltage": "false", "FrameIndex": 0, "PRIME-Port": "CMOS", "555 AO-MinVolts": "0.0000", "COM3-Description": "Serial port driver (boost:asio)", "528 AO-Description": "NI DAC", "555 AO-IOChannel": "Dev1/ao6", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "555 AO-TriggerInputLine": "/Dev1/PFI0", "PRIME-ActualInterval-ms": "84.6200", "LudlController-Port": "COM9", "445 Shutter-Demo": "No", "PRIME-Trigger-Expose Out-Mux": "1", "SliceIndex": 8, "PRIME-CCDTemperatureSetPoint": "-15.0000", "405 Shutter-MinVolts": "0.0000", "PRIME-ExposeOutMode": "First Row", "AxisPositions": null, "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Volts": "0.0000", "528-Name": "DA Shutter", "555-State": "0", "XLIGHT Dichroic-Label": "QUAD", "445 AO-Sequenceable": "1", "405-Name": "DA Shutter", "405 AO-IOChannel": "Dev1/ao0", "Core-Camera": "PRIME", "Focus-Name": "Focus", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "405 Shutter-IOChannel": "Dev1/ao8", "640 AO-Demo": "No", "640 Shutter-SupportsTriggering": "0", "445 AO-Demo": "No", "555-DA Device": "555 Shutter", "470 Shutter-Description": "NI DAC", "528 AO-Volts": "0.0000", "COM1-DataBits": "8", "Vincent-D3-Address": "x", "520 AO-SupportsTriggering": "0", "405-Description": "DA device that is used as a shutter", "640 Shutter-MinVolts": "0.0000", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "405 AO-Name": "AnalogIO", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "640 AO-TriggerInputLine": "/Dev1/PFI0", "405 AO-TriggerInputLine": "/Dev1/PFI0", "640-Description": "DA device that is used as a shutter", "445 Shutter-TriggerSampleRate": "100000", "470 Shutter-TriggerSequenceLength": "1000", "COM1-StopBits": "1", "520 Shutter-SupportsTriggering": "0", "405-State": "1", "Core-Initialize": "1", "XLIGHT Emission Wheel-State": "3", "520 AO-Description": "NI DAC", "528 AO-TriggerInputLine": "/Dev1/PFI0", "445 Shutter-MaxVolts": "5.0000", "COM9-Handshaking": "Off", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "XLIGHT Spin motor-Label": "On (spinning)", "PRIME-PP 5 ENABLED": "No", "UUID": "29f6996d-3db3-4a08-b413-15250699dedc", "PRIME-TriggerMode": "Internal Trigger", "COM1-Handshaking": "Off", "PRIME-Timing-PostTriggerDelayNs": "49344000.0000", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "Focus-Position": "16732.9000", "528 Shutter-Block voltage": "false", "Channel": "P-DAPI-high", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "555 Shutter-Demo": "No", "405 Shutter-Demo": "No", "528 AO-Name": "AnalogIO", "520-State": "0", "Core-Galvo": "", "405 Shutter-TriggerSampleRate": "100000", "Focus-FocusStepSize_(nm)": "100", "PRIME-PixelType": "16bit", "COM4-DataBits": "8", "470 AO-IOChannel": "Dev1/ao2", "520 AO-MinVolts": "0.0000", "COM9-Parity": "None", "PRIME-CircularBufferEnabled": "ON", "PRIME-PP 6 Reset": "No", "640 AO-Description": "NI DAC", "Source": "PRIME", "445 AO-MinVolts": "0.0000", "PIEZO-Name": "Stage", "445 AO-Description": "NI DAC", "PRIME-CircularBufferFrameCount": "46", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "520 AO-Volts": "0.0000", "528 Shutter-TriggerSampleRate": "100000", "XPositionUm": 23269.100000000002, "OpticalPath-State": "4", "PRIME-PP 3 THRESHOLD": "80", "PRIME-PVCAM Version": "3.7.1", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Gain": "1-HDR", "640 Shutter-TriggerSequenceLength": "1000", "528 AO-SupportsTriggering": "0", "445 AO-TriggerInputLine": "/Dev1/PFI0", "OpticalPath-Name": "OpticalPath", "PRIME-PP 4 ENABLED": "Yes", "PRIME-PVCAM Adapter Version": "1.3.29", "COM3-Name": "COM3", "405 AO-MaxVolts": "5.0000", "520 Shutter-Block voltage": "false", "PRIME-Timing-ClearingTimeNs": "49344000.0000", "Core-Focus": "PIEZO", "528 AO-Block voltage": "false", "TE2000-Description": "Nikon TE2000 hub", "528 AO-MinVolts": "0.0000", "COM4-Parity": "None", "Summary": { "Slices": 11, "ChContrastMin": [ 0, 0, 0, 0, 0, 0 ], "PixelAspect": 1, "SlicesFirst": true, "Interval_ms": 0, "Time": "2019-12-17 03:59:33 -0500", "Prefix": "seq_7", "InitialPositionList": [ { "Label": "Pos0", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [149.91], "Focus": [16657.7], "XYStage": [ 71629.7, 19152.9 ] }, "GridColumnIndex": 0 }, { "Label": "Pos1", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [190.82], "Focus": [16485.4], "XYStage": [ 65702.3, 19396.600000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos2", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [194.69], "Focus": [16446.8], "XYStage": [ 59880.5, 19007.8 ] }, "GridColumnIndex": 0 }, { "Label": "Pos3", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [145.04], "Focus": [16446.6], "XYStage": [ 53616.9, 18818.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos4", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [169.57500000000002], "Focus": [16446.5], "XYStage": [ 47662.8, 18817.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos5", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [131.805], "Focus": [16446.4], "XYStage": [ 41918.8, 18298.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos6", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [113.145], "Focus": [16446.5], "XYStage": [ 36129.9, 18895.8 ] }, "GridColumnIndex": 0 }, { "Label": "Pos7", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [141.005], "Focus": [16446.6], "XYStage": [ 30299.100000000002, 18895.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos8", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [140.97], "Focus": [16446.5], "XYStage": [ 23952.100000000002, 19156 ] }, "GridColumnIndex": 0 }, { "Label": "Pos9", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [184.57500000000002], "Focus": [16677.9], "XYStage": [ 72866.8, -14809.300000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos10", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [167.95000000000002], "Focus": [16557.9], "XYStage": [ 66515.6, -14371.300000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos11", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [168], "Focus": [16618.9], "XYStage": [ 59724.3, -13964.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos12", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [203.205], "Focus": [16779.6], "XYStage": [ 53862.3, -13494.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos13", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [203.20000000000002], "Focus": [16749], "XYStage": [ 48108.3, -13805.400000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos14", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [203.19], "Focus": [16675.9], "XYStage": [ 41645, -13804.900000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos15", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [203.21], "Focus": [16675.7], "XYStage": [ 35352.6, -13805.6 ] }, "GridColumnIndex": 0 }, { "Label": "Pos16", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [203.225], "Focus": [16675.8], "XYStage": [ 29298, -13447.400000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos17", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [203.215], "Focus": [16675.8], "XYStage": [ 23269.100000000002, -13012.300000000001 ] }, "GridColumnIndex": 0 } ], "z-step_um": 10, "GridRow": 0, "BitDepth": 16, "Depth": 2, "Channels": 6, "Source": "Micro-Manager", "ChColors": [ -16711681, -256, -65281, -1, -1, -16776961 ], "MetadataVersion": 10, "KeepShutterOpenChannels": true, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536, 65536 ], "CustomIntervals_ms": [], "Height": 1200, "MicroManagerVersion": "1.4.23 20180219", "GridColumn": 0, "UUID": "3ad8801b-5699-40d6-813b-4ad840863e38", "PixelType": "GRAY16", "Width": 1200, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\Yan\\20191216-YC113-S11S12-miseq", "Frames": 1, "Comment": "", "UserName": "Nikon", "PixelSize_um": 1.1, "ROI": [ 0, 0, 1200, 1200 ], "Date": "2019-12-17", "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC", "P-DAPI-high" ], "KeepShutterOpenSlices": true, "IJType": 1, "ComputerName": "ZadorConfocal2", "Positions": 18, "PositionIndex": 17, "CameraTimeout": 20000, "TimeFirst": false }, "640 AO-Name": "AnalogIO", "Vincent-D3-Command": "Close" }, "FrameKey-0-5-9": { "COM4-StopBits": "1", "528 AO-MaxVolts": "5.0000", "XLIGHT Excitation Wheel-State": "1", "470 Shutter-Name": "AnalogIO", "Core-Shutter": "405", "555 AO-Demo": "No", "XLIGHT Dichroic-State": "2", "XYStage-StepSize-Y": "0.1000", "ZPositionUm": 243.215, "XYStage-StepSize-X": "0.1000", "PRIME-FirmwareVersion": "20.28", "PRIME-ClearCycles": "2", "520-Name": "DA Shutter", "405 Shutter-Name": "AnalogIO", "PIEZO-StepSize": "0.0050", "PRIME-ClearMode": "Pre-Exposure", "470 AO-MinVolts": "0.0000", "405 AO-SupportsTriggering": "1", "CameraChannelIndex": 0, "XYStage-StartSpeed": "500.0000", "COM9-StopBits": "2", "405 AO-Description": "NI DAC", "COM4-DelayBetweenCharsMs": "0.0000", "445 AO-Block voltage": "false", "520 AO-TriggerInputLine": "/Dev1/PFI0", "555 AO-Description": "NI DAC", "520 AO-TriggerSampleRate": "100000", "COM4-Verbose": "1", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM4-Name": "COM4", "405 Shutter-Volts": "5.0000", "Vincent-D3-Port": "COM1", "PRIME-PP 4 MIN ADU AFFECTED": "200", "470 Shutter-MinVolts": "0.0000", "PRIME-PP 3 ENABLED": "Yes", "470 AO-Description": "NI DAC", "PRIME-Description": "PVCAM API device adapter", "520 Shutter-MaxVolts": "5.0000", "520 Shutter-Volts": "0.0000", "528 AO-Sequenceable": "1", "PRIME-Binning": "1x1", "555 Shutter-MaxVolts": "5.0000", "528 Shutter-TriggerSequenceLength": "1000", "405 Shutter-Block voltage": "false", "XLIGHT Spin motor-State": "1", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "Vincent-D3-Time to open (ms)": "35.0000", "Vincent-D3-Time to close (ms)": "35.0000", "445 AO-TriggerSampleRate": "100000", "640 Shutter-IOChannel": "Dev1/ao14", "555 AO-Sequenceable": "1", "520 AO-Name": "AnalogIO", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "470 AO-Block voltage": "false", "COM1-Verbose": "1", "COM9-AnswerTimeout": "10000.0000", "528 Shutter-Name": "AnalogIO", "PRIME-Trigger Timeout (secs)": "10", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "NextFrame": 0, "470 AO-TriggerSequenceLength": "1000", "PRIME-TransposeXY": "0", "445 AO-TriggerSequenceLength": "1000", "COM1-Name": "COM1", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "COM9-DataBits": "8", "COM1-AnswerTimeout": "500.0000", "Camera": "", "TE2000-Version": "V2.50-V2.50-V2.10", "528 Shutter-Volts": "0.0000", "XLIGHT Hub-Name": "XLIGHT Hub", "PIEZO-Description": "Ludl stage driver adapter", "PositionName": "Pos17", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSampleRate": "100000", "PRIME-FanSpeedSetpoint": "High", "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "405 AO-TriggerSampleRate": "100000", "470 Shutter-Block voltage": "false", "470-State": "0", "COM3-Parity": "None", "405 AO-Volts": "4.5000", "PRIME-TransposeMirrorY": "0", "PRIME-ChipName": "GS144BSI", "470 AO-Volts": "0.0000", "PRIME-TransposeMirrorX": "0", "445 Shutter-SupportsTriggering": "0", "470 Shutter-SupportsTriggering": "0", "XLIGHT Hub-Description": "XLIGHT hub", "PRIME-PreampOffLimit": "10000", "470 AO-Sequenceable": "1", "528 Shutter-MaxVolts": "5.0000", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "Core-XYStage": "XYStage", "470 AO-TriggerSampleRate": "100000", "PRIME-Timing-PreTriggerDelayNs": "24672000.0000", "528-DA Device": "528 Shutter", "Focus-Description": "Nikon TE2000 focus adapter", "FilterBlock-State": "5", "520 AO-Demo": "No", "COM3-DelayBetweenCharsMs": "0.0000", "470 Shutter-Volts": "0.0000", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "PRIME-PP 2 THRESHOLD": "125", "PRIME-PP 4 THRESHOLD": "75", "XYStage-Acceleration": "4.0000", "470 Shutter-Demo": "No", "PRIME-Name": "PMPCIECam00", "640-DA Device": "640 Shutter", "405 AO-Block voltage": "false", "ElapsedTime-ms": 753019, "PRIME-Offset": "100", "445 AO-IOChannel": "Dev1/ao1", "640 AO-MaxVolts": "5.0000", "PositionIndex": 17, "PRIME-PP 1 ENABLED": "Yes", "COM1-BaudRate": "9600", "640-State": "0", "COM3-Verbose": "1", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "LudlController-TransmissionDelay": "0", "PRIME-FullWellCapacity": "62000", "PRIME-SMARTStreamingEnabled": "No", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "640 Shutter-Description": "NI DAC", "528-Description": "DA device that is used as a shutter", "COM4-Description": "Serial port driver (boost:asio)", "405 Shutter-Description": "NI DAC", "TE2000-Name": "TE2000", "528 Shutter-Description": "NI DAC", "COM9-Description": "Serial port driver (boost:asio)", "LudlController-Name": "LudlController", "445 Shutter-TriggerSequenceLength": "1000", "PRIME-SerialNumber": "A18B203008", "445 AO-Volts": "0.0000", "PRIME-X-dimension": "1200", "PRIME-ShutterCloseDelay": "0", "XLIGHT Disk slider-State": "2", "Position": "Default", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 AO-IOChannel": "Dev1/ao5", "520 AO-IOChannel": "Dev1/ao4", "528 AO-TriggerSequenceLength": "1000", "405 Shutter-MaxVolts": "5.0000", "470-DA Device": "470 Shutter", "PRIME-FTCapable": "No", "Core-ChannelGroup": "Channels", "555 AO-SupportsTriggering": "0", "PRIME-Y-dimension": "1200", "Frame": 0, "555 Shutter-Sequenceable": "1", "PRIME-Timing-ExposureTimeNs": "499998000.0000", "Core-ImageProcessor": "", "528-State": "0", "520 AO-Block voltage": "false", "XLIGHT Excitation Wheel-Label": "QUAD", "640 Shutter-Demo": "No", "COM3-StopBits": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "520 Shutter-IOChannel": "Dev1/ao11", "Width": 1200, "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-SupportsTriggering": "0", "640 AO-TriggerSampleRate": "100000", "520-Description": "DA device that is used as a shutter", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "405 Shutter-Sequenceable": "1", "405 Shutter-SupportsTriggering": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-TriggerSampleRate": "100000", "640 Shutter-MaxVolts": "5.0000", "555 AO-MaxVolts": "5.0000", "520 AO-TriggerSequenceLength": "1000", "COM3-BaudRate": "9600", "COM9-BaudRate": "9600", "Vincent-D3-Channel #": "Ch. #1", "PRIME-PMode": "Normal", "OpticalPath-Label": "Left(100)", "BitDepth": 16, "555 AO-TriggerSequenceLength": "1000", "445 Shutter-Description": "NI DAC", "405 AO-Sequenceable": "1", "PRIME-AcquisitionMethod": "Callbacks", "555 AO-Name": "AnalogIO", "PRIME-ShutterMode": "Pre-Exposure", "COM3-Handshaking": "Off", "445 AO-SupportsTriggering": "0", "ROI": "0-0-1200-1200", "COM9-Verbose": "1", "PRIME-Color": "OFF", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "PRIME-ShutterOpenDelay": "0", "COM1-Parity": "None", "Slice": 9, "SlicePosition": 40, "COM9-DelayBetweenCharsMs": "0.0000", "555-Name": "DA Shutter", "PIEZO-LudlSingleAxisName": "B", "XLIGHT Emission Wheel-Label": "QUAD", "640 Shutter-Name": "AnalogIO", "640 AO-Sequenceable": "1", "520 Shutter-Demo": "No", "528 Shutter-Demo": "No", "470-Name": "DA Shutter", "520-DA Device": "520 Shutter", "XYStage-Description": "Ludl XY stage driver adapter", "445 Shutter-MinVolts": "0.0000", "528 AO-TriggerSampleRate": "100000", "405 AO-MinVolts": "0.0000", "405 Shutter-TriggerSequenceLength": "1000", "Height": 1200, "555 AO-Block voltage": "false", "640 Shutter-Volts": "0.0000", "PixelType": "GRAY16", "445 Shutter-Block voltage": "false", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "520 Shutter-TriggerSequenceLength": "1000", "COM9-Name": "COM9", "Core-SLM": "", "555 AO-Volts": "0.0000", "555 Shutter-Block voltage": "false", "XYStage-Speed": "1800.0000", "520 AO-MaxVolts": "5.0000", "528 Shutter-SupportsTriggering": "0", "COM1-Description": "Serial port driver (boost:asio)", "PRIME-MetadataEnabled": "No", "520 Shutter-MinVolts": "0.0000", "470 Shutter-Sequenceable": "1", "PRIME-PreampDelay": "0", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-MinVolts": "0.0000", "ChannelIndex": 5, "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Core-AutoShutter": "1", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "Exposure-ms": 300, "PRIME-PP 2 ENABLED": "Yes", "640 Shutter-TriggerSampleRate": "100000", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "470 Shutter-IOChannel": "Dev1/ao10", "405 AO-Demo": "No", "PixelSizeUm": 1.1, "470 AO-Demo": "No", "470 Shutter-MaxVolts": "5.0000", "Vincent-D3-Name": "Vincent-D3", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FileName": "img_000000000_P-DAPI-high_009.tif", "LudlController-Reset": "Operate", "PRIME-Exposure": "300.00", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "445-Name": "DA Shutter", "555 Shutter-Name": "AnalogIO", "520 Shutter-TriggerSampleRate": "100000", "445 Shutter-IOChannel": "Dev1/ao9", "PRIME-TransposeCorrection": "0", "XYStage-StepSize": "0.1000", "445-Description": "DA device that is used as a shutter", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 AO-IOChannel": "Dev1/ao7", "520 Shutter-Name": "AnalogIO", "COM3-DataBits": "8", "640 AO-Volts": "0.0000", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "555 Shutter-IOChannel": "Dev1/ao13", "WaitInterval": null, "520 Shutter-Sequenceable": "1", "YPositionUm": -13012.300000000001, "445-State": "0", "445-DA Device": "445 Shutter", "PRIME-CircularBufferAutoSize": "ON", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "555 AO-TriggerSampleRate": "100000", "445 Shutter-Volts": "0.0000", "XLIGHT Hub-Port": "COM3", "Core-AutoFocus": "", "COM4-Handshaking": "Off", "528 Shutter-MinVolts": "0.0000", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "445 Shutter-Name": "AnalogIO", "528 Shutter-Sequenceable": "1", "XYStage-TransposeMirrorX": "0", "XYStage-TransposeMirrorY": "0", "PRIME-ReadoutRate": "100MHz 16bit", "XYStage-Name": "Stage", "640 AO-SupportsTriggering": "0", "640-Name": "DA Shutter", "640 Shutter-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "640 AO-Block voltage": "false", "445 Shutter-Sequenceable": "1", "470 AO-MaxVolts": "5.0000", "COM4-BaudRate": "9600", "470-Description": "DA device that is used as a shutter", "520 Shutter-Description": "NI DAC", "PRIME-CCDTemperature": "-14.9200", "Time": "2019-12-17 04:00:10 -0500", "640 AO-TriggerSequenceLength": "1000", "470 AO-Name": "AnalogIO", "XLIGHT Disk slider-Label": "Disk Pos 40um", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "Binning": "1x1", "TE2000-ModelType": "1", "COM3-AnswerTimeout": "500.0000", "555 Shutter-SupportsTriggering": "0", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "TE2000-Port": "COM4", "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Description": "NI DAC", "COM4-AnswerTimeout": "500.0000", "COM1-DelayBetweenCharsMs": "0.0000", "FilterBlock-Label": "Confocal", "445 AO-Name": "AnalogIO", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-Timing-ReadoutTimeNs": "24672000.0000", "528 AO-Demo": "No", "520 AO-Sequenceable": "1", "555-Description": "DA device that is used as a shutter", "405-DA Device": "405 Shutter", "405 AO-TriggerSequenceLength": "1000", "640 AO-MinVolts": "0.0000", "640 Shutter-Block voltage": "false", "FrameIndex": 0, "PRIME-Port": "CMOS", "555 AO-MinVolts": "0.0000", "COM3-Description": "Serial port driver (boost:asio)", "528 AO-Description": "NI DAC", "555 AO-IOChannel": "Dev1/ao6", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "555 AO-TriggerInputLine": "/Dev1/PFI0", "PRIME-ActualInterval-ms": "84.6200", "LudlController-Port": "COM9", "445 Shutter-Demo": "No", "PRIME-Trigger-Expose Out-Mux": "1", "SliceIndex": 9, "PRIME-CCDTemperatureSetPoint": "-15.0000", "405 Shutter-MinVolts": "0.0000", "PRIME-ExposeOutMode": "First Row", "AxisPositions": null, "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Volts": "0.0000", "528-Name": "DA Shutter", "555-State": "0", "XLIGHT Dichroic-Label": "QUAD", "445 AO-Sequenceable": "1", "405-Name": "DA Shutter", "405 AO-IOChannel": "Dev1/ao0", "Core-Camera": "PRIME", "Focus-Name": "Focus", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "405 Shutter-IOChannel": "Dev1/ao8", "640 AO-Demo": "No", "640 Shutter-SupportsTriggering": "0", "445 AO-Demo": "No", "555-DA Device": "555 Shutter", "470 Shutter-Description": "NI DAC", "528 AO-Volts": "0.0000", "COM1-DataBits": "8", "Vincent-D3-Address": "x", "520 AO-SupportsTriggering": "0", "405-Description": "DA device that is used as a shutter", "640 Shutter-MinVolts": "0.0000", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "405 AO-Name": "AnalogIO", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "640 AO-TriggerInputLine": "/Dev1/PFI0", "405 AO-TriggerInputLine": "/Dev1/PFI0", "640-Description": "DA device that is used as a shutter", "445 Shutter-TriggerSampleRate": "100000", "470 Shutter-TriggerSequenceLength": "1000", "COM1-StopBits": "1", "520 Shutter-SupportsTriggering": "0", "405-State": "1", "Core-Initialize": "1", "XLIGHT Emission Wheel-State": "3", "520 AO-Description": "NI DAC", "528 AO-TriggerInputLine": "/Dev1/PFI0", "445 Shutter-MaxVolts": "5.0000", "COM9-Handshaking": "Off", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "XLIGHT Spin motor-Label": "On (spinning)", "PRIME-PP 5 ENABLED": "No", "UUID": "792994aa-f790-4696-beb5-a66d45a51cf3", "PRIME-TriggerMode": "Internal Trigger", "COM1-Handshaking": "Off", "PRIME-Timing-PostTriggerDelayNs": "49344000.0000", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "Focus-Position": "16732.9000", "528 Shutter-Block voltage": "false", "Channel": "P-DAPI-high", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "555 Shutter-Demo": "No", "405 Shutter-Demo": "No", "528 AO-Name": "AnalogIO", "520-State": "0", "Core-Galvo": "", "405 Shutter-TriggerSampleRate": "100000", "Focus-FocusStepSize_(nm)": "100", "PRIME-PixelType": "16bit", "COM4-DataBits": "8", "470 AO-IOChannel": "Dev1/ao2", "520 AO-MinVolts": "0.0000", "COM9-Parity": "None", "PRIME-CircularBufferEnabled": "ON", "PRIME-PP 6 Reset": "No", "640 AO-Description": "NI DAC", "Source": "PRIME", "445 AO-MinVolts": "0.0000", "PIEZO-Name": "Stage", "445 AO-Description": "NI DAC", "PRIME-CircularBufferFrameCount": "46", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "520 AO-Volts": "0.0000", "528 Shutter-TriggerSampleRate": "100000", "XPositionUm": 23269.100000000002, "OpticalPath-State": "4", "PRIME-PP 3 THRESHOLD": "80", "PRIME-PVCAM Version": "3.7.1", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Gain": "1-HDR", "640 Shutter-TriggerSequenceLength": "1000", "528 AO-SupportsTriggering": "0", "445 AO-TriggerInputLine": "/Dev1/PFI0", "OpticalPath-Name": "OpticalPath", "PRIME-PP 4 ENABLED": "Yes", "PRIME-PVCAM Adapter Version": "1.3.29", "COM3-Name": "COM3", "405 AO-MaxVolts": "5.0000", "520 Shutter-Block voltage": "false", "PRIME-Timing-ClearingTimeNs": "49344000.0000", "Core-Focus": "PIEZO", "528 AO-Block voltage": "false", "TE2000-Description": "Nikon TE2000 hub", "528 AO-MinVolts": "0.0000", "COM4-Parity": "None", "Summary": { "Slices": 11, "ChContrastMin": [ 0, 0, 0, 0, 0, 0 ], "PixelAspect": 1, "SlicesFirst": true, "Interval_ms": 0, "Time": "2019-12-17 03:59:33 -0500", "Prefix": "seq_7", "InitialPositionList": [ { "Label": "Pos0", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [149.91], "Focus": [16657.7], "XYStage": [ 71629.7, 19152.9 ] }, "GridColumnIndex": 0 }, { "Label": "Pos1", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [190.82], "Focus": [16485.4], "XYStage": [ 65702.3, 19396.600000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos2", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [194.69], "Focus": [16446.8], "XYStage": [ 59880.5, 19007.8 ] }, "GridColumnIndex": 0 }, { "Label": "Pos3", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [145.04], "Focus": [16446.6], "XYStage": [ 53616.9, 18818.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos4", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [169.57500000000002], "Focus": [16446.5], "XYStage": [ 47662.8, 18817.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos5", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [131.805], "Focus": [16446.4], "XYStage": [ 41918.8, 18298.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos6", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [113.145], "Focus": [16446.5], "XYStage": [ 36129.9, 18895.8 ] }, "GridColumnIndex": 0 }, { "Label": "Pos7", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [141.005], "Focus": [16446.6], "XYStage": [ 30299.100000000002, 18895.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos8", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [140.97], "Focus": [16446.5], "XYStage": [ 23952.100000000002, 19156 ] }, "GridColumnIndex": 0 }, { "Label": "Pos9", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [184.57500000000002], "Focus": [16677.9], "XYStage": [ 72866.8, -14809.300000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos10", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [167.95000000000002], "Focus": [16557.9], "XYStage": [ 66515.6, -14371.300000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos11", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [168], "Focus": [16618.9], "XYStage": [ 59724.3, -13964.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos12", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [203.205], "Focus": [16779.6], "XYStage": [ 53862.3, -13494.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos13", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [203.20000000000002], "Focus": [16749], "XYStage": [ 48108.3, -13805.400000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos14", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [203.19], "Focus": [16675.9], "XYStage": [ 41645, -13804.900000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos15", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [203.21], "Focus": [16675.7], "XYStage": [ 35352.6, -13805.6 ] }, "GridColumnIndex": 0 }, { "Label": "Pos16", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [203.225], "Focus": [16675.8], "XYStage": [ 29298, -13447.400000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos17", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [203.215], "Focus": [16675.8], "XYStage": [ 23269.100000000002, -13012.300000000001 ] }, "GridColumnIndex": 0 } ], "z-step_um": 10, "GridRow": 0, "BitDepth": 16, "Depth": 2, "Channels": 6, "Source": "Micro-Manager", "ChColors": [ -16711681, -256, -65281, -1, -1, -16776961 ], "MetadataVersion": 10, "KeepShutterOpenChannels": true, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536, 65536 ], "CustomIntervals_ms": [], "Height": 1200, "MicroManagerVersion": "1.4.23 20180219", "GridColumn": 0, "UUID": "3ad8801b-5699-40d6-813b-4ad840863e38", "PixelType": "GRAY16", "Width": 1200, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\Yan\\20191216-YC113-S11S12-miseq", "Frames": 1, "Comment": "", "UserName": "Nikon", "PixelSize_um": 1.1, "ROI": [ 0, 0, 1200, 1200 ], "Date": "2019-12-17", "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC", "P-DAPI-high" ], "KeepShutterOpenSlices": true, "IJType": 1, "ComputerName": "ZadorConfocal2", "Positions": 18, "PositionIndex": 17, "CameraTimeout": 20000, "TimeFirst": false }, "640 AO-Name": "AnalogIO", "Vincent-D3-Command": "Close" }, "FrameKey-0-5-10": { "COM4-StopBits": "1", "528 AO-MaxVolts": "5.0000", "XLIGHT Excitation Wheel-State": "1", "470 Shutter-Name": "AnalogIO", "Core-Shutter": "405", "555 AO-Demo": "No", "XLIGHT Dichroic-State": "2", "XYStage-StepSize-Y": "0.1000", "ZPositionUm": 253.215, "XYStage-StepSize-X": "0.1000", "PRIME-FirmwareVersion": "20.28", "PRIME-ClearCycles": "2", "520-Name": "DA Shutter", "405 Shutter-Name": "AnalogIO", "PIEZO-StepSize": "0.0050", "PRIME-ClearMode": "Pre-Exposure", "470 AO-MinVolts": "0.0000", "405 AO-SupportsTriggering": "1", "CameraChannelIndex": 0, "XYStage-StartSpeed": "500.0000", "COM9-StopBits": "2", "405 AO-Description": "NI DAC", "COM4-DelayBetweenCharsMs": "0.0000", "445 AO-Block voltage": "false", "520 AO-TriggerInputLine": "/Dev1/PFI0", "555 AO-Description": "NI DAC", "520 AO-TriggerSampleRate": "100000", "COM4-Verbose": "1", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM4-Name": "COM4", "405 Shutter-Volts": "5.0000", "Vincent-D3-Port": "COM1", "PRIME-PP 4 MIN ADU AFFECTED": "200", "470 Shutter-MinVolts": "0.0000", "PRIME-PP 3 ENABLED": "Yes", "470 AO-Description": "NI DAC", "PRIME-Description": "PVCAM API device adapter", "520 Shutter-MaxVolts": "5.0000", "520 Shutter-Volts": "0.0000", "528 AO-Sequenceable": "1", "PRIME-Binning": "1x1", "555 Shutter-MaxVolts": "5.0000", "528 Shutter-TriggerSequenceLength": "1000", "405 Shutter-Block voltage": "false", "XLIGHT Spin motor-State": "1", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "Vincent-D3-Time to open (ms)": "35.0000", "Vincent-D3-Time to close (ms)": "35.0000", "445 AO-TriggerSampleRate": "100000", "640 Shutter-IOChannel": "Dev1/ao14", "555 AO-Sequenceable": "1", "520 AO-Name": "AnalogIO", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "470 AO-Block voltage": "false", "COM1-Verbose": "1", "COM9-AnswerTimeout": "10000.0000", "528 Shutter-Name": "AnalogIO", "PRIME-Trigger Timeout (secs)": "10", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "NextFrame": null, "470 AO-TriggerSequenceLength": "1000", "PRIME-TransposeXY": "0", "445 AO-TriggerSequenceLength": "1000", "COM1-Name": "COM1", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "COM9-DataBits": "8", "COM1-AnswerTimeout": "500.0000", "Camera": "", "TE2000-Version": "V2.50-V2.50-V2.10", "528 Shutter-Volts": "0.0000", "XLIGHT Hub-Name": "XLIGHT Hub", "PIEZO-Description": "Ludl stage driver adapter", "PositionName": "Pos17", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSampleRate": "100000", "PRIME-FanSpeedSetpoint": "High", "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "405 AO-TriggerSampleRate": "100000", "470 Shutter-Block voltage": "false", "470-State": "0", "COM3-Parity": "None", "405 AO-Volts": "4.5000", "PRIME-TransposeMirrorY": "0", "PRIME-ChipName": "GS144BSI", "470 AO-Volts": "0.0000", "PRIME-TransposeMirrorX": "0", "445 Shutter-SupportsTriggering": "0", "470 Shutter-SupportsTriggering": "0", "XLIGHT Hub-Description": "XLIGHT hub", "PRIME-PreampOffLimit": "10000", "470 AO-Sequenceable": "1", "528 Shutter-MaxVolts": "5.0000", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "Core-XYStage": "XYStage", "470 AO-TriggerSampleRate": "100000", "PRIME-Timing-PreTriggerDelayNs": "24672000.0000", "528-DA Device": "528 Shutter", "Focus-Description": "Nikon TE2000 focus adapter", "FilterBlock-State": "5", "520 AO-Demo": "No", "COM3-DelayBetweenCharsMs": "0.0000", "470 Shutter-Volts": "0.0000", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "PRIME-PP 2 THRESHOLD": "125", "PRIME-PP 4 THRESHOLD": "75", "XYStage-Acceleration": "4.0000", "470 Shutter-Demo": "No", "PRIME-Name": "PMPCIECam00", "640-DA Device": "640 Shutter", "405 AO-Block voltage": "false", "ElapsedTime-ms": 753567, "PRIME-Offset": "100", "445 AO-IOChannel": "Dev1/ao1", "640 AO-MaxVolts": "5.0000", "PositionIndex": 17, "PRIME-PP 1 ENABLED": "Yes", "COM1-BaudRate": "9600", "640-State": "0", "COM3-Verbose": "1", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "LudlController-TransmissionDelay": "0", "PRIME-FullWellCapacity": "62000", "PRIME-SMARTStreamingEnabled": "No", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "640 Shutter-Description": "NI DAC", "528-Description": "DA device that is used as a shutter", "COM4-Description": "Serial port driver (boost:asio)", "405 Shutter-Description": "NI DAC", "TE2000-Name": "TE2000", "528 Shutter-Description": "NI DAC", "COM9-Description": "Serial port driver (boost:asio)", "LudlController-Name": "LudlController", "445 Shutter-TriggerSequenceLength": "1000", "PRIME-SerialNumber": "A18B203008", "445 AO-Volts": "0.0000", "PRIME-X-dimension": "1200", "PRIME-ShutterCloseDelay": "0", "XLIGHT Disk slider-State": "2", "Position": "Default", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 AO-IOChannel": "Dev1/ao5", "520 AO-IOChannel": "Dev1/ao4", "528 AO-TriggerSequenceLength": "1000", "405 Shutter-MaxVolts": "5.0000", "470-DA Device": "470 Shutter", "PRIME-FTCapable": "No", "Core-ChannelGroup": "Channels", "555 AO-SupportsTriggering": "0", "PRIME-Y-dimension": "1200", "Frame": 0, "555 Shutter-Sequenceable": "1", "PRIME-Timing-ExposureTimeNs": "499998000.0000", "Core-ImageProcessor": "", "528-State": "0", "520 AO-Block voltage": "false", "XLIGHT Excitation Wheel-Label": "QUAD", "640 Shutter-Demo": "No", "COM3-StopBits": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "520 Shutter-IOChannel": "Dev1/ao11", "Width": 1200, "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-SupportsTriggering": "0", "640 AO-TriggerSampleRate": "100000", "520-Description": "DA device that is used as a shutter", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "405 Shutter-Sequenceable": "1", "405 Shutter-SupportsTriggering": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-TriggerSampleRate": "100000", "640 Shutter-MaxVolts": "5.0000", "555 AO-MaxVolts": "5.0000", "520 AO-TriggerSequenceLength": "1000", "COM3-BaudRate": "9600", "COM9-BaudRate": "9600", "Vincent-D3-Channel #": "Ch. #1", "PRIME-PMode": "Normal", "OpticalPath-Label": "Left(100)", "BitDepth": 16, "555 AO-TriggerSequenceLength": "1000", "445 Shutter-Description": "NI DAC", "405 AO-Sequenceable": "1", "PRIME-AcquisitionMethod": "Callbacks", "555 AO-Name": "AnalogIO", "PRIME-ShutterMode": "Pre-Exposure", "COM3-Handshaking": "Off", "445 AO-SupportsTriggering": "0", "ROI": "0-0-1200-1200", "COM9-Verbose": "1", "PRIME-Color": "OFF", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "PRIME-ShutterOpenDelay": "0", "COM1-Parity": "None", "Slice": 10, "SlicePosition": 50, "COM9-DelayBetweenCharsMs": "0.0000", "555-Name": "DA Shutter", "PIEZO-LudlSingleAxisName": "B", "XLIGHT Emission Wheel-Label": "QUAD", "640 Shutter-Name": "AnalogIO", "640 AO-Sequenceable": "1", "520 Shutter-Demo": "No", "528 Shutter-Demo": "No", "470-Name": "DA Shutter", "520-DA Device": "520 Shutter", "XYStage-Description": "Ludl XY stage driver adapter", "445 Shutter-MinVolts": "0.0000", "528 AO-TriggerSampleRate": "100000", "405 AO-MinVolts": "0.0000", "405 Shutter-TriggerSequenceLength": "1000", "Height": 1200, "555 AO-Block voltage": "false", "640 Shutter-Volts": "0.0000", "PixelType": "GRAY16", "445 Shutter-Block voltage": "false", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "520 Shutter-TriggerSequenceLength": "1000", "COM9-Name": "COM9", "Core-SLM": "", "555 AO-Volts": "0.0000", "555 Shutter-Block voltage": "false", "XYStage-Speed": "1800.0000", "520 AO-MaxVolts": "5.0000", "528 Shutter-SupportsTriggering": "0", "COM1-Description": "Serial port driver (boost:asio)", "PRIME-MetadataEnabled": "No", "520 Shutter-MinVolts": "0.0000", "470 Shutter-Sequenceable": "1", "PRIME-PreampDelay": "0", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-MinVolts": "0.0000", "ChannelIndex": 5, "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Core-AutoShutter": "1", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "Exposure-ms": 300, "PRIME-PP 2 ENABLED": "Yes", "640 Shutter-TriggerSampleRate": "100000", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "470 Shutter-IOChannel": "Dev1/ao10", "405 AO-Demo": "No", "PixelSizeUm": 1.1, "470 AO-Demo": "No", "470 Shutter-MaxVolts": "5.0000", "Vincent-D3-Name": "Vincent-D3", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FileName": "img_000000000_P-DAPI-high_010.tif", "LudlController-Reset": "Operate", "PRIME-Exposure": "300.00", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "445-Name": "DA Shutter", "555 Shutter-Name": "AnalogIO", "520 Shutter-TriggerSampleRate": "100000", "445 Shutter-IOChannel": "Dev1/ao9", "PRIME-TransposeCorrection": "0", "XYStage-StepSize": "0.1000", "445-Description": "DA device that is used as a shutter", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 AO-IOChannel": "Dev1/ao7", "520 Shutter-Name": "AnalogIO", "COM3-DataBits": "8", "640 AO-Volts": "0.0000", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "555 Shutter-IOChannel": "Dev1/ao13", "WaitInterval": null, "520 Shutter-Sequenceable": "1", "YPositionUm": -13012.300000000001, "445-State": "0", "445-DA Device": "445 Shutter", "PRIME-CircularBufferAutoSize": "ON", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "555 AO-TriggerSampleRate": "100000", "445 Shutter-Volts": "0.0000", "XLIGHT Hub-Port": "COM3", "Core-AutoFocus": "", "COM4-Handshaking": "Off", "528 Shutter-MinVolts": "0.0000", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "445 Shutter-Name": "AnalogIO", "528 Shutter-Sequenceable": "1", "XYStage-TransposeMirrorX": "0", "XYStage-TransposeMirrorY": "0", "PRIME-ReadoutRate": "100MHz 16bit", "XYStage-Name": "Stage", "640 AO-SupportsTriggering": "0", "640-Name": "DA Shutter", "640 Shutter-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "640 AO-Block voltage": "false", "445 Shutter-Sequenceable": "1", "470 AO-MaxVolts": "5.0000", "COM4-BaudRate": "9600", "470-Description": "DA device that is used as a shutter", "520 Shutter-Description": "NI DAC", "PRIME-CCDTemperature": "-14.9200", "Time": "2019-12-17 04:00:10 -0500", "640 AO-TriggerSequenceLength": "1000", "470 AO-Name": "AnalogIO", "XLIGHT Disk slider-Label": "Disk Pos 40um", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "Binning": "1x1", "TE2000-ModelType": "1", "COM3-AnswerTimeout": "500.0000", "555 Shutter-SupportsTriggering": "0", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "TE2000-Port": "COM4", "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Description": "NI DAC", "COM4-AnswerTimeout": "500.0000", "COM1-DelayBetweenCharsMs": "0.0000", "FilterBlock-Label": "Confocal", "445 AO-Name": "AnalogIO", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-Timing-ReadoutTimeNs": "24672000.0000", "528 AO-Demo": "No", "520 AO-Sequenceable": "1", "555-Description": "DA device that is used as a shutter", "405-DA Device": "405 Shutter", "405 AO-TriggerSequenceLength": "1000", "640 AO-MinVolts": "0.0000", "640 Shutter-Block voltage": "false", "FrameIndex": 0, "PRIME-Port": "CMOS", "555 AO-MinVolts": "0.0000", "COM3-Description": "Serial port driver (boost:asio)", "528 AO-Description": "NI DAC", "555 AO-IOChannel": "Dev1/ao6", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "555 AO-TriggerInputLine": "/Dev1/PFI0", "PRIME-ActualInterval-ms": "84.6200", "LudlController-Port": "COM9", "445 Shutter-Demo": "No", "PRIME-Trigger-Expose Out-Mux": "1", "SliceIndex": 10, "PRIME-CCDTemperatureSetPoint": "-15.0000", "405 Shutter-MinVolts": "0.0000", "PRIME-ExposeOutMode": "First Row", "AxisPositions": null, "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Volts": "0.0000", "528-Name": "DA Shutter", "555-State": "0", "XLIGHT Dichroic-Label": "QUAD", "445 AO-Sequenceable": "1", "405-Name": "DA Shutter", "405 AO-IOChannel": "Dev1/ao0", "Core-Camera": "PRIME", "Focus-Name": "Focus", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "405 Shutter-IOChannel": "Dev1/ao8", "640 AO-Demo": "No", "640 Shutter-SupportsTriggering": "0", "445 AO-Demo": "No", "555-DA Device": "555 Shutter", "470 Shutter-Description": "NI DAC", "528 AO-Volts": "0.0000", "COM1-DataBits": "8", "Vincent-D3-Address": "x", "520 AO-SupportsTriggering": "0", "405-Description": "DA device that is used as a shutter", "640 Shutter-MinVolts": "0.0000", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "405 AO-Name": "AnalogIO", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "640 AO-TriggerInputLine": "/Dev1/PFI0", "405 AO-TriggerInputLine": "/Dev1/PFI0", "640-Description": "DA device that is used as a shutter", "445 Shutter-TriggerSampleRate": "100000", "470 Shutter-TriggerSequenceLength": "1000", "COM1-StopBits": "1", "520 Shutter-SupportsTriggering": "0", "405-State": "0", "Core-Initialize": "1", "XLIGHT Emission Wheel-State": "3", "520 AO-Description": "NI DAC", "528 AO-TriggerInputLine": "/Dev1/PFI0", "445 Shutter-MaxVolts": "5.0000", "COM9-Handshaking": "Off", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "XLIGHT Spin motor-Label": "On (spinning)", "PRIME-PP 5 ENABLED": "No", "UUID": "f2a66151-1a2c-46c9-9211-b2b559a6d2e1", "PRIME-TriggerMode": "Internal Trigger", "COM1-Handshaking": "Off", "PRIME-Timing-PostTriggerDelayNs": "49344000.0000", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "Focus-Position": "16732.9000", "528 Shutter-Block voltage": "false", "Channel": "P-DAPI-high", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "555 Shutter-Demo": "No", "405 Shutter-Demo": "No", "528 AO-Name": "AnalogIO", "520-State": "0", "Core-Galvo": "", "405 Shutter-TriggerSampleRate": "100000", "Focus-FocusStepSize_(nm)": "100", "PRIME-PixelType": "16bit", "COM4-DataBits": "8", "470 AO-IOChannel": "Dev1/ao2", "520 AO-MinVolts": "0.0000", "COM9-Parity": "None", "PRIME-CircularBufferEnabled": "ON", "PRIME-PP 6 Reset": "No", "640 AO-Description": "NI DAC", "Source": "PRIME", "445 AO-MinVolts": "0.0000", "PIEZO-Name": "Stage", "445 AO-Description": "NI DAC", "PRIME-CircularBufferFrameCount": "46", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "520 AO-Volts": "0.0000", "528 Shutter-TriggerSampleRate": "100000", "XPositionUm": 23269.100000000002, "OpticalPath-State": "4", "PRIME-PP 3 THRESHOLD": "80", "PRIME-PVCAM Version": "3.7.1", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Gain": "1-HDR", "640 Shutter-TriggerSequenceLength": "1000", "528 AO-SupportsTriggering": "0", "445 AO-TriggerInputLine": "/Dev1/PFI0", "OpticalPath-Name": "OpticalPath", "PRIME-PP 4 ENABLED": "Yes", "PRIME-PVCAM Adapter Version": "1.3.29", "COM3-Name": "COM3", "405 AO-MaxVolts": "5.0000", "520 Shutter-Block voltage": "false", "PRIME-Timing-ClearingTimeNs": "49344000.0000", "Core-Focus": "PIEZO", "528 AO-Block voltage": "false", "TE2000-Description": "Nikon TE2000 hub", "528 AO-MinVolts": "0.0000", "COM4-Parity": "None", "Summary": { "Slices": 11, "ChContrastMin": [ 0, 0, 0, 0, 0, 0 ], "PixelAspect": 1, "SlicesFirst": true, "Interval_ms": 0, "Time": "2019-12-17 03:59:33 -0500", "Prefix": "seq_7", "InitialPositionList": [ { "Label": "Pos0", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [149.91], "Focus": [16657.7], "XYStage": [ 71629.7, 19152.9 ] }, "GridColumnIndex": 0 }, { "Label": "Pos1", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [190.82], "Focus": [16485.4], "XYStage": [ 65702.3, 19396.600000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos2", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [194.69], "Focus": [16446.8], "XYStage": [ 59880.5, 19007.8 ] }, "GridColumnIndex": 0 }, { "Label": "Pos3", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [145.04], "Focus": [16446.6], "XYStage": [ 53616.9, 18818.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos4", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [169.57500000000002], "Focus": [16446.5], "XYStage": [ 47662.8, 18817.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos5", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [131.805], "Focus": [16446.4], "XYStage": [ 41918.8, 18298.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos6", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [113.145], "Focus": [16446.5], "XYStage": [ 36129.9, 18895.8 ] }, "GridColumnIndex": 0 }, { "Label": "Pos7", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [141.005], "Focus": [16446.6], "XYStage": [ 30299.100000000002, 18895.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos8", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [140.97], "Focus": [16446.5], "XYStage": [ 23952.100000000002, 19156 ] }, "GridColumnIndex": 0 }, { "Label": "Pos9", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [184.57500000000002], "Focus": [16677.9], "XYStage": [ 72866.8, -14809.300000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos10", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [167.95000000000002], "Focus": [16557.9], "XYStage": [ 66515.6, -14371.300000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos11", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [168], "Focus": [16618.9], "XYStage": [ 59724.3, -13964.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos12", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [203.205], "Focus": [16779.6], "XYStage": [ 53862.3, -13494.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos13", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [203.20000000000002], "Focus": [16749], "XYStage": [ 48108.3, -13805.400000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos14", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [203.19], "Focus": [16675.9], "XYStage": [ 41645, -13804.900000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos15", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [203.21], "Focus": [16675.7], "XYStage": [ 35352.6, -13805.6 ] }, "GridColumnIndex": 0 }, { "Label": "Pos16", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [203.225], "Focus": [16675.8], "XYStage": [ 29298, -13447.400000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos17", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [203.215], "Focus": [16675.8], "XYStage": [ 23269.100000000002, -13012.300000000001 ] }, "GridColumnIndex": 0 } ], "z-step_um": 10, "GridRow": 0, "BitDepth": 16, "Depth": 2, "Channels": 6, "Source": "Micro-Manager", "ChColors": [ -16711681, -256, -65281, -1, -1, -16776961 ], "MetadataVersion": 10, "KeepShutterOpenChannels": true, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536, 65536 ], "CustomIntervals_ms": [], "Height": 1200, "MicroManagerVersion": "1.4.23 20180219", "GridColumn": 0, "UUID": "3ad8801b-5699-40d6-813b-4ad840863e38", "PixelType": "GRAY16", "Width": 1200, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\Yan\\20191216-YC113-S11S12-miseq", "Frames": 1, "Comment": "", "UserName": "Nikon", "PixelSize_um": 1.1, "ROI": [ 0, 0, 1200, 1200 ], "Date": "2019-12-17", "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC", "P-DAPI-high" ], "KeepShutterOpenSlices": true, "IJType": 1, "ComputerName": "ZadorConfocal2", "Positions": 18, "PositionIndex": 17, "CameraTimeout": 20000, "TimeFirst": false }, "640 AO-Name": "AnalogIO", "Vincent-D3-Command": "Close" } }