{ "Summary": { "Slices": 11, "ChContrastMin": [ 0, 0, 0, 0, 0, 0 ], "PixelAspect": 1, "SlicesFirst": true, "Interval_ms": 0, "Time": "2019-12-17 21:58:39 -0500", "Prefix": "seq_3", "InitialPositionList": [ { "Label": "Pos0", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.435], "Focus": [16480.2], "XYStage": [ 69822.40000000001, 20100.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos1", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.425], "Focus": [16480.3], "XYStage": [ 69004, 20101 ] }, "GridColumnIndex": 0 }, { "Label": "Pos2", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.44], "Focus": [16480.1], "XYStage": [ 63119.5, 20690.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos3", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.405], "Focus": [16480.2], "XYStage": [ 62321, 20690.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos4", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.415], "Focus": [16480], "XYStage": [ 56604.100000000006, 20688.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos5", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.42000000000002], "Focus": [16480.1], "XYStage": [ 56034.200000000004, 20688.800000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos6", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.455], "Focus": [16480], "XYStage": [ 50889.3, 20496.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos7", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.43], "Focus": [16480.2], "XYStage": [ 50101.600000000006, 20496.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos8", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.67000000000002], "Focus": [16479.9], "XYStage": [ 44818.600000000006, 20496.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos9", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.67000000000002], "Focus": [16480], "XYStage": [ 43897.9, 20436.800000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos10", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.675], "Focus": [16479.9], "XYStage": [ 39670.600000000006, 20664.9 ] }, "GridColumnIndex": 0 }, { "Label": "Pos11", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.69], "Focus": [16479.9], "XYStage": [ 38734, 20664.600000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos12", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.695], "Focus": [16480.1], "XYStage": [ 34037.200000000004, 20664.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos13", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.685], "Focus": [16480.1], "XYStage": [ 33152, 20664.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos14", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.69], "Focus": [16480.1], "XYStage": [ 28122.800000000003, 20664.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos15", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.675], "Focus": [16480], "XYStage": [ 27422.9, 20664.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos16", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.64000000000001], "Focus": [16480], "XYStage": [ 23005.2, 20664.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos17", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.69], "Focus": [16479.9], "XYStage": [ 21820.600000000002, 20409.600000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos18", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.41], "Focus": [16480.1], "XYStage": [ 73697.6, -13222.800000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos19", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.455], "Focus": [16480.1], "XYStage": [ 72765.8, -13223 ] }, "GridColumnIndex": 0 }, { "Label": "Pos20", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.4], "Focus": [16480.2], "XYStage": [ 67445.5, -12303.800000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos21", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.415], "Focus": [16480.2], "XYStage": [ 66613.3, -12303.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos22", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.415], "Focus": [16480.1], "XYStage": [ 61819.4, -11936.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos23", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.405], "Focus": [16480.2], "XYStage": [ 61325.700000000004, -11937.1 ] }, "GridColumnIndex": 0 }, { "Label": "Pos24", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.46], "Focus": [16480.2], "XYStage": [ 55377, -10974.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos25", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.445], "Focus": [16480.1], "XYStage": [ 54827, -11214.800000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos26", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.435], "Focus": [16480.2], "XYStage": [ 49937.100000000006, -10919.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos27", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.425], "Focus": [16480.1], "XYStage": [ 48764, -10919.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos28", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.42000000000002], "Focus": [16480.2], "XYStage": [ 43978.4, -10920.400000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos29", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.435], "Focus": [16480.2], "XYStage": [ 43106.3, -10920.400000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos30", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.405], "Focus": [16480.3], "XYStage": [ 38212.4, -10920.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos31", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.445], "Focus": [16480.2], "XYStage": [ 37254.9, -10920.400000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos32", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.44], "Focus": [16480.3], "XYStage": [ 32472.800000000003, -11294.400000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos33", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [166.95000000000002], "Focus": [16480.2], "XYStage": [ 31420.7, -11294.400000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos34", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [166.95000000000002], "Focus": [16480.2], "XYStage": [ 26881.5, -11640 ] }, "GridColumnIndex": 0 }, { "Label": "Pos35", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [166.98], "Focus": [16480.2], "XYStage": [ 25876.100000000002, -11641.1 ] }, "GridColumnIndex": 0 } ], "z-step_um": 10, "GridRow": 0, "BitDepth": 16, "Depth": 2, "Channels": 6, "Source": "Micro-Manager", "ChColors": [ -16711681, -256, -65281, -1, -1, -16776961 ], "MetadataVersion": 10, "KeepShutterOpenChannels": true, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536, 65536 ], "CustomIntervals_ms": [], "Height": 1200, "MicroManagerVersion": "1.4.23 20180219", "GridColumn": 0, "UUID": "8c8f9d20-b128-4577-9f91-89e70f22d319", "PixelType": "GRAY16", "Width": 1200, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\Yan\\20191217-YC113-S5S6", "Frames": 1, "Comment": "", "UserName": "Nikon", "PixelSize_um": 1.1, "ROI": [ 0, 0, 1200, 1200 ], "Date": "2019-12-17", "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC", "P-DAPI-high" ], "KeepShutterOpenSlices": true, "IJType": 1, "ComputerName": "ZadorConfocal2", "Positions": 36, "PositionIndex": 3, "CameraTimeout": 20000, "TimeFirst": false }, "FrameKey-0-0-0": { "COM4-StopBits": "1", "528 AO-MaxVolts": "5.0000", "XLIGHT Excitation Wheel-State": "2", "470 Shutter-Name": "AnalogIO", "Core-Shutter": "520", "555 AO-Demo": "No", "XLIGHT Dichroic-State": "2", "XYStage-StepSize-Y": "0.1000", "ZPositionUm": 127.405, "XYStage-StepSize-X": "0.1000", "PRIME-FirmwareVersion": "20.28", "PRIME-ClearCycles": "2", "520-Name": "DA Shutter", "405 Shutter-Name": "AnalogIO", "PIEZO-StepSize": "0.0050", "PRIME-ClearMode": "Pre-Exposure", "470 AO-MinVolts": "0.0000", "405 AO-SupportsTriggering": "1", "CameraChannelIndex": 0, "XYStage-StartSpeed": "500.0000", "COM9-StopBits": "2", "405 AO-Description": "NI DAC", "COM4-DelayBetweenCharsMs": "0.0000", "445 AO-Block voltage": "false", "520 AO-TriggerInputLine": "/Dev1/PFI0", "555 AO-Description": "NI DAC", "520 AO-TriggerSampleRate": "100000", "COM4-Verbose": "1", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM4-Name": "COM4", "405 Shutter-Volts": "0.0000", "Vincent-D3-Port": "COM1", "PRIME-PP 4 MIN ADU AFFECTED": "200", "470 Shutter-MinVolts": "0.0000", "PRIME-PP 3 ENABLED": "Yes", "470 AO-Description": "NI DAC", "PRIME-Description": "PVCAM API device adapter", "520 Shutter-MaxVolts": "5.0000", "520 Shutter-Volts": "5.0000", "528 AO-Sequenceable": "1", "PRIME-Binning": "1x1", "555 Shutter-MaxVolts": "5.0000", "528 Shutter-TriggerSequenceLength": "1000", "405 Shutter-Block voltage": "false", "XLIGHT Spin motor-State": "1", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "Vincent-D3-Time to open (ms)": "35.0000", "Vincent-D3-Time to close (ms)": "35.0000", "445 AO-TriggerSampleRate": "100000", "640 Shutter-IOChannel": "Dev1/ao14", "555 AO-Sequenceable": "1", "520 AO-Name": "AnalogIO", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "470 AO-Block voltage": "false", "COM1-Verbose": "1", "COM9-AnswerTimeout": "10000.0000", "528 Shutter-Name": "AnalogIO", "PRIME-Trigger Timeout (secs)": "10", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "NextFrame": 0, "470 AO-TriggerSequenceLength": "1000", "PRIME-TransposeXY": "0", "445 AO-TriggerSequenceLength": "1000", "COM1-Name": "COM1", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "COM9-DataBits": "8", "COM1-AnswerTimeout": "500.0000", "Camera": "", "TE2000-Version": "V2.50-V2.50-V2.10", "528 Shutter-Volts": "0.0000", "XLIGHT Hub-Name": "XLIGHT Hub", "PIEZO-Description": "Ludl stage driver adapter", "PositionName": "Pos3", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSampleRate": "100000", "PRIME-FanSpeedSetpoint": "High", "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "405 AO-TriggerSampleRate": "100000", "470 Shutter-Block voltage": "false", "470-State": "0", "COM3-Parity": "None", "405 AO-Volts": "0.0000", "PRIME-TransposeMirrorY": "0", "PRIME-ChipName": "GS144BSI", "470 AO-Volts": "0.0000", "PRIME-TransposeMirrorX": "0", "445 Shutter-SupportsTriggering": "0", "470 Shutter-SupportsTriggering": "0", "XLIGHT Hub-Description": "XLIGHT hub", "PRIME-PreampOffLimit": "10000", "470 AO-Sequenceable": "1", "528 Shutter-MaxVolts": "5.0000", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "Core-XYStage": "XYStage", "470 AO-TriggerSampleRate": "100000", "PRIME-Timing-PreTriggerDelayNs": "24672000.0000", "528-DA Device": "528 Shutter", "Focus-Description": "Nikon TE2000 focus adapter", "FilterBlock-State": "5", "520 AO-Demo": "No", "COM3-DelayBetweenCharsMs": "0.0000", "470 Shutter-Volts": "0.0000", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "PRIME-PP 2 THRESHOLD": "125", "PRIME-PP 4 THRESHOLD": "75", "XYStage-Acceleration": "4.0000", "470 Shutter-Demo": "No", "PRIME-Name": "PMPCIECam00", "640-DA Device": "640 Shutter", "405 AO-Block voltage": "false", "ElapsedTime-ms": 132339, "PRIME-Offset": "100", "445 AO-IOChannel": "Dev1/ao1", "640 AO-MaxVolts": "5.0000", "PositionIndex": 3, "PRIME-PP 1 ENABLED": "Yes", "COM1-BaudRate": "9600", "640-State": "0", "COM3-Verbose": "1", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "LudlController-TransmissionDelay": "0", "PRIME-FullWellCapacity": "62000", "PRIME-SMARTStreamingEnabled": "No", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "640 Shutter-Description": "NI DAC", "528-Description": "DA device that is used as a shutter", "COM4-Description": "Serial port driver (boost:asio)", "405 Shutter-Description": "NI DAC", "TE2000-Name": "TE2000", "528 Shutter-Description": "NI DAC", "COM9-Description": "Serial port driver (boost:asio)", "LudlController-Name": "LudlController", "445 Shutter-TriggerSequenceLength": "1000", "PRIME-SerialNumber": "A18B203008", "445 AO-Volts": "0.0000", "PRIME-X-dimension": "1200", "PRIME-ShutterCloseDelay": "0", "XLIGHT Disk slider-State": "2", "Position": "Default", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 AO-IOChannel": "Dev1/ao5", "520 AO-IOChannel": "Dev1/ao4", "528 AO-TriggerSequenceLength": "1000", "405 Shutter-MaxVolts": "5.0000", "470-DA Device": "470 Shutter", "PRIME-FTCapable": "No", "Core-ChannelGroup": "Channels", "555 AO-SupportsTriggering": "0", "PRIME-Y-dimension": "1200", "Frame": 0, "555 Shutter-Sequenceable": "1", "PRIME-Timing-ExposureTimeNs": "9992000.0000", "Core-ImageProcessor": "", "528-State": "0", "520 AO-Block voltage": "false", "XLIGHT Excitation Wheel-Label": "CFP/YFP", "640 Shutter-Demo": "No", "COM3-StopBits": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "520 Shutter-IOChannel": "Dev1/ao11", "Width": 1200, "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-SupportsTriggering": "0", "640 AO-TriggerSampleRate": "100000", "520-Description": "DA device that is used as a shutter", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "405 Shutter-Sequenceable": "1", "405 Shutter-SupportsTriggering": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-TriggerSampleRate": "100000", "640 Shutter-MaxVolts": "5.0000", "555 AO-MaxVolts": "5.0000", "520 AO-TriggerSequenceLength": "1000", "COM3-BaudRate": "9600", "COM9-BaudRate": "9600", "Vincent-D3-Channel #": "Ch. #1", "PRIME-PMode": "Normal", "OpticalPath-Label": "Left(100)", "BitDepth": 16, "555 AO-TriggerSequenceLength": "1000", "445 Shutter-Description": "NI DAC", "405 AO-Sequenceable": "1", "PRIME-AcquisitionMethod": "Callbacks", "555 AO-Name": "AnalogIO", "PRIME-ShutterMode": "Pre-Exposure", "COM3-Handshaking": "Off", "445 AO-SupportsTriggering": "0", "ROI": "0-0-1200-1200", "COM9-Verbose": "1", "PRIME-Color": "OFF", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "PRIME-ShutterOpenDelay": "0", "COM1-Parity": "None", "Slice": 0, "SlicePosition": -50, "COM9-DelayBetweenCharsMs": "0.0000", "555-Name": "DA Shutter", "PIEZO-LudlSingleAxisName": "B", "XLIGHT Emission Wheel-Label": "565/24(G)", "640 Shutter-Name": "AnalogIO", "640 AO-Sequenceable": "1", "520 Shutter-Demo": "No", "528 Shutter-Demo": "No", "470-Name": "DA Shutter", "520-DA Device": "520 Shutter", "XYStage-Description": "Ludl XY stage driver adapter", "445 Shutter-MinVolts": "0.0000", "528 AO-TriggerSampleRate": "100000", "405 AO-MinVolts": "0.0000", "405 Shutter-TriggerSequenceLength": "1000", "Height": 1200, "555 AO-Block voltage": "false", "640 Shutter-Volts": "0.0000", "PixelType": "GRAY16", "445 Shutter-Block voltage": "false", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "520 Shutter-TriggerSequenceLength": "1000", "COM9-Name": "COM9", "Core-SLM": "", "555 AO-Volts": "0.0000", "555 Shutter-Block voltage": "false", "XYStage-Speed": "1800.0000", "520 AO-MaxVolts": "5.0000", "528 Shutter-SupportsTriggering": "0", "COM1-Description": "Serial port driver (boost:asio)", "PRIME-MetadataEnabled": "No", "520 Shutter-MinVolts": "0.0000", "470 Shutter-Sequenceable": "1", "PRIME-PreampDelay": "0", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-MinVolts": "0.0000", "ChannelIndex": 0, "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Core-AutoShutter": "1", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "Exposure-ms": 320, "PRIME-PP 2 ENABLED": "Yes", "640 Shutter-TriggerSampleRate": "100000", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "470 Shutter-IOChannel": "Dev1/ao10", "405 AO-Demo": "No", "PixelSizeUm": 1.1, "470 AO-Demo": "No", "470 Shutter-MaxVolts": "5.0000", "Vincent-D3-Name": "Vincent-D3", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FileName": "img_000000000_Il-G_000.tif", "LudlController-Reset": "Operate", "PRIME-Exposure": "320.00", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "445-Name": "DA Shutter", "555 Shutter-Name": "AnalogIO", "520 Shutter-TriggerSampleRate": "100000", "445 Shutter-IOChannel": "Dev1/ao9", "PRIME-TransposeCorrection": "0", "XYStage-StepSize": "0.1000", "445-Description": "DA device that is used as a shutter", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 AO-IOChannel": "Dev1/ao7", "520 Shutter-Name": "AnalogIO", "COM3-DataBits": "8", "640 AO-Volts": "0.0000", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "555 Shutter-IOChannel": "Dev1/ao13", "WaitInterval": null, "520 Shutter-Sequenceable": "1", "YPositionUm": 20690.300000000003, "445-State": "0", "445-DA Device": "445 Shutter", "PRIME-CircularBufferAutoSize": "ON", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "555 AO-TriggerSampleRate": "100000", "445 Shutter-Volts": "0.0000", "XLIGHT Hub-Port": "COM3", "Core-AutoFocus": "", "COM4-Handshaking": "Off", "528 Shutter-MinVolts": "0.0000", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "445 Shutter-Name": "AnalogIO", "528 Shutter-Sequenceable": "1", "XYStage-TransposeMirrorX": "0", "XYStage-TransposeMirrorY": "0", "PRIME-ReadoutRate": "100MHz 16bit", "XYStage-Name": "Stage", "640 AO-SupportsTriggering": "0", "640-Name": "DA Shutter", "640 Shutter-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "640 AO-Block voltage": "false", "445 Shutter-Sequenceable": "1", "470 AO-MaxVolts": "5.0000", "COM4-BaudRate": "9600", "470-Description": "DA device that is used as a shutter", "520 Shutter-Description": "NI DAC", "PRIME-CCDTemperature": "-14.9600", "Time": "2019-12-17 21:58:39 -0500", "640 AO-TriggerSequenceLength": "1000", "470 AO-Name": "AnalogIO", "XLIGHT Disk slider-Label": "Disk Pos 40um", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "Binning": "1x1", "TE2000-ModelType": "1", "COM3-AnswerTimeout": "500.0000", "555 Shutter-SupportsTriggering": "0", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "TE2000-Port": "COM4", "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Description": "NI DAC", "COM4-AnswerTimeout": "500.0000", "COM1-DelayBetweenCharsMs": "0.0000", "FilterBlock-Label": "Confocal", "445 AO-Name": "AnalogIO", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-Timing-ReadoutTimeNs": "24672000.0000", "528 AO-Demo": "No", "520 AO-Sequenceable": "1", "555-Description": "DA device that is used as a shutter", "405-DA Device": "405 Shutter", "405 AO-TriggerSequenceLength": "1000", "640 AO-MinVolts": "0.0000", "640 Shutter-Block voltage": "false", "FrameIndex": 0, "PRIME-Port": "CMOS", "555 AO-MinVolts": "0.0000", "COM3-Description": "Serial port driver (boost:asio)", "528 AO-Description": "NI DAC", "555 AO-IOChannel": "Dev1/ao6", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "555 AO-TriggerInputLine": "/Dev1/PFI0", "PRIME-ActualInterval-ms": "374.3700", "LudlController-Port": "COM9", "445 Shutter-Demo": "No", "PRIME-Trigger-Expose Out-Mux": "1", "SliceIndex": 0, "PRIME-CCDTemperatureSetPoint": "-15.0000", "405 Shutter-MinVolts": "0.0000", "PRIME-ExposeOutMode": "First Row", "AxisPositions": null, "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Volts": "0.0000", "528-Name": "DA Shutter", "555-State": "0", "XLIGHT Dichroic-Label": "CFP/YFP", "445 AO-Sequenceable": "1", "405-Name": "DA Shutter", "405 AO-IOChannel": "Dev1/ao0", "Core-Camera": "PRIME", "Focus-Name": "Focus", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "405 Shutter-IOChannel": "Dev1/ao8", "640 AO-Demo": "No", "640 Shutter-SupportsTriggering": "0", "445 AO-Demo": "No", "555-DA Device": "555 Shutter", "470 Shutter-Description": "NI DAC", "528 AO-Volts": "0.0000", "COM1-DataBits": "8", "Vincent-D3-Address": "x", "520 AO-SupportsTriggering": "0", "405-Description": "DA device that is used as a shutter", "640 Shutter-MinVolts": "0.0000", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "405 AO-Name": "AnalogIO", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "640 AO-TriggerInputLine": "/Dev1/PFI0", "405 AO-TriggerInputLine": "/Dev1/PFI0", "640-Description": "DA device that is used as a shutter", "445 Shutter-TriggerSampleRate": "100000", "470 Shutter-TriggerSequenceLength": "1000", "COM1-StopBits": "1", "520 Shutter-SupportsTriggering": "0", "405-State": "0", "Core-Initialize": "1", "XLIGHT Emission Wheel-State": "7", "520 AO-Description": "NI DAC", "528 AO-TriggerInputLine": "/Dev1/PFI0", "445 Shutter-MaxVolts": "5.0000", "COM9-Handshaking": "Off", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "XLIGHT Spin motor-Label": "On (spinning)", "PRIME-PP 5 ENABLED": "No", "UUID": "da96a4bf-2388-49d9-bfb7-542f0b47b3b3", "PRIME-TriggerMode": "Internal Trigger", "COM1-Handshaking": "Off", "PRIME-Timing-PostTriggerDelayNs": "49344000.0000", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "Focus-Position": "16479.8000", "528 Shutter-Block voltage": "false", "Channel": "Il-G", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "555 Shutter-Demo": "No", "405 Shutter-Demo": "No", "528 AO-Name": "AnalogIO", "520-State": "1", "Core-Galvo": "", "405 Shutter-TriggerSampleRate": "100000", "Focus-FocusStepSize_(nm)": "100", "PRIME-PixelType": "16bit", "COM4-DataBits": "8", "470 AO-IOChannel": "Dev1/ao2", "520 AO-MinVolts": "0.0000", "COM9-Parity": "None", "PRIME-CircularBufferEnabled": "ON", "PRIME-PP 6 Reset": "No", "640 AO-Description": "NI DAC", "Source": "PRIME", "445 AO-MinVolts": "0.0000", "PIEZO-Name": "Stage", "445 AO-Description": "NI DAC", "PRIME-CircularBufferFrameCount": "46", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "520 AO-Volts": "4.5000", "528 Shutter-TriggerSampleRate": "100000", "XPositionUm": 62321, "OpticalPath-State": "4", "PRIME-PP 3 THRESHOLD": "80", "PRIME-PVCAM Version": "3.7.1", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Gain": "1-HDR", "640 Shutter-TriggerSequenceLength": "1000", "528 AO-SupportsTriggering": "0", "445 AO-TriggerInputLine": "/Dev1/PFI0", "OpticalPath-Name": "OpticalPath", "PRIME-PP 4 ENABLED": "Yes", "PRIME-PVCAM Adapter Version": "1.3.29", "COM3-Name": "COM3", "405 AO-MaxVolts": "5.0000", "520 Shutter-Block voltage": "false", "PRIME-Timing-ClearingTimeNs": "49344000.0000", "Core-Focus": "PIEZO", "528 AO-Block voltage": "false", "TE2000-Description": "Nikon TE2000 hub", "528 AO-MinVolts": "0.0000", "COM4-Parity": "None", "Summary": { "Slices": 11, "ChContrastMin": [ 0, 0, 0, 0, 0, 0 ], "PixelAspect": 1, "SlicesFirst": true, "Interval_ms": 0, "Time": "2019-12-17 21:58:39 -0500", "Prefix": "seq_3", "InitialPositionList": [ { "Label": "Pos0", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.435], "Focus": [16480.2], "XYStage": [ 69822.40000000001, 20100.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos1", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.425], "Focus": [16480.3], "XYStage": [ 69004, 20101 ] }, "GridColumnIndex": 0 }, { "Label": "Pos2", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.44], "Focus": [16480.1], "XYStage": [ 63119.5, 20690.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos3", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.405], "Focus": [16480.2], "XYStage": [ 62321, 20690.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos4", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.415], "Focus": [16480], "XYStage": [ 56604.100000000006, 20688.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos5", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.42000000000002], "Focus": [16480.1], "XYStage": [ 56034.200000000004, 20688.800000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos6", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.455], "Focus": [16480], "XYStage": [ 50889.3, 20496.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos7", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.43], "Focus": [16480.2], "XYStage": [ 50101.600000000006, 20496.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos8", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.67000000000002], "Focus": [16479.9], "XYStage": [ 44818.600000000006, 20496.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos9", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.67000000000002], "Focus": [16480], "XYStage": [ 43897.9, 20436.800000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos10", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.675], "Focus": [16479.9], "XYStage": [ 39670.600000000006, 20664.9 ] }, "GridColumnIndex": 0 }, { "Label": "Pos11", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.69], "Focus": [16479.9], "XYStage": [ 38734, 20664.600000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos12", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.695], "Focus": [16480.1], "XYStage": [ 34037.200000000004, 20664.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos13", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.685], "Focus": [16480.1], "XYStage": [ 33152, 20664.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos14", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.69], "Focus": [16480.1], "XYStage": [ 28122.800000000003, 20664.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos15", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.675], "Focus": [16480], "XYStage": [ 27422.9, 20664.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos16", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.64000000000001], "Focus": [16480], "XYStage": [ 23005.2, 20664.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos17", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.69], "Focus": [16479.9], "XYStage": [ 21820.600000000002, 20409.600000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos18", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.41], "Focus": [16480.1], "XYStage": [ 73697.6, -13222.800000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos19", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.455], "Focus": [16480.1], "XYStage": [ 72765.8, -13223 ] }, "GridColumnIndex": 0 }, { "Label": "Pos20", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.4], "Focus": [16480.2], "XYStage": [ 67445.5, -12303.800000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos21", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.415], "Focus": [16480.2], "XYStage": [ 66613.3, -12303.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos22", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.415], "Focus": [16480.1], "XYStage": [ 61819.4, -11936.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos23", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.405], "Focus": [16480.2], "XYStage": [ 61325.700000000004, -11937.1 ] }, "GridColumnIndex": 0 }, { "Label": "Pos24", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.46], "Focus": [16480.2], "XYStage": [ 55377, -10974.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos25", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.445], "Focus": [16480.1], "XYStage": [ 54827, -11214.800000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos26", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.435], "Focus": [16480.2], "XYStage": [ 49937.100000000006, -10919.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos27", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.425], "Focus": [16480.1], "XYStage": [ 48764, -10919.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos28", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.42000000000002], "Focus": [16480.2], "XYStage": [ 43978.4, -10920.400000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos29", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.435], "Focus": [16480.2], "XYStage": [ 43106.3, -10920.400000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos30", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.405], "Focus": [16480.3], "XYStage": [ 38212.4, -10920.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos31", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.445], "Focus": [16480.2], "XYStage": [ 37254.9, -10920.400000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos32", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.44], "Focus": [16480.3], "XYStage": [ 32472.800000000003, -11294.400000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos33", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [166.95000000000002], "Focus": [16480.2], "XYStage": [ 31420.7, -11294.400000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos34", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [166.95000000000002], "Focus": [16480.2], "XYStage": [ 26881.5, -11640 ] }, "GridColumnIndex": 0 }, { "Label": "Pos35", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [166.98], "Focus": [16480.2], "XYStage": [ 25876.100000000002, -11641.1 ] }, "GridColumnIndex": 0 } ], "z-step_um": 10, "GridRow": 0, "BitDepth": 16, "Depth": 2, "Channels": 6, "Source": "Micro-Manager", "ChColors": [ -16711681, -256, -65281, -1, -1, -16776961 ], "MetadataVersion": 10, "KeepShutterOpenChannels": true, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536, 65536 ], "CustomIntervals_ms": [], "Height": 1200, "MicroManagerVersion": "1.4.23 20180219", "GridColumn": 0, "UUID": "8c8f9d20-b128-4577-9f91-89e70f22d319", "PixelType": "GRAY16", "Width": 1200, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\Yan\\20191217-YC113-S5S6", "Frames": 1, "Comment": "", "UserName": "Nikon", "PixelSize_um": 1.1, "ROI": [ 0, 0, 1200, 1200 ], "Date": "2019-12-17", "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC", "P-DAPI-high" ], "KeepShutterOpenSlices": true, "IJType": 1, "ComputerName": "ZadorConfocal2", "Positions": 36, "PositionIndex": 3, "CameraTimeout": 20000, "TimeFirst": false }, "640 AO-Name": "AnalogIO", "Vincent-D3-Command": "Close" }, "FrameKey-0-0-1": { "COM4-StopBits": "1", "528 AO-MaxVolts": "5.0000", "XLIGHT Excitation Wheel-State": "2", "470 Shutter-Name": "AnalogIO", "Core-Shutter": "520", "555 AO-Demo": "No", "XLIGHT Dichroic-State": "2", "XYStage-StepSize-Y": "0.1000", "ZPositionUm": 137.405, "XYStage-StepSize-X": "0.1000", "PRIME-FirmwareVersion": "20.28", "PRIME-ClearCycles": "2", "520-Name": "DA Shutter", "405 Shutter-Name": "AnalogIO", "PIEZO-StepSize": "0.0050", "PRIME-ClearMode": "Pre-Exposure", "470 AO-MinVolts": "0.0000", "405 AO-SupportsTriggering": "1", "CameraChannelIndex": 0, "XYStage-StartSpeed": "500.0000", "COM9-StopBits": "2", "405 AO-Description": "NI DAC", "COM4-DelayBetweenCharsMs": "0.0000", "445 AO-Block voltage": "false", "520 AO-TriggerInputLine": "/Dev1/PFI0", "555 AO-Description": "NI DAC", "520 AO-TriggerSampleRate": "100000", "COM4-Verbose": "1", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM4-Name": "COM4", "405 Shutter-Volts": "0.0000", "Vincent-D3-Port": "COM1", "PRIME-PP 4 MIN ADU AFFECTED": "200", "470 Shutter-MinVolts": "0.0000", "PRIME-PP 3 ENABLED": "Yes", "470 AO-Description": "NI DAC", "PRIME-Description": "PVCAM API device adapter", "520 Shutter-MaxVolts": "5.0000", "520 Shutter-Volts": "5.0000", "528 AO-Sequenceable": "1", "PRIME-Binning": "1x1", "555 Shutter-MaxVolts": "5.0000", "528 Shutter-TriggerSequenceLength": "1000", "405 Shutter-Block voltage": "false", "XLIGHT Spin motor-State": "1", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "Vincent-D3-Time to open (ms)": "35.0000", "Vincent-D3-Time to close (ms)": "35.0000", "445 AO-TriggerSampleRate": "100000", "640 Shutter-IOChannel": "Dev1/ao14", "555 AO-Sequenceable": "1", "520 AO-Name": "AnalogIO", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "470 AO-Block voltage": "false", "COM1-Verbose": "1", "COM9-AnswerTimeout": "10000.0000", "528 Shutter-Name": "AnalogIO", "PRIME-Trigger Timeout (secs)": "10", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "NextFrame": 0, "470 AO-TriggerSequenceLength": "1000", "PRIME-TransposeXY": "0", "445 AO-TriggerSequenceLength": "1000", "COM1-Name": "COM1", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "COM9-DataBits": "8", "COM1-AnswerTimeout": "500.0000", "Camera": "", "TE2000-Version": "V2.50-V2.50-V2.10", "528 Shutter-Volts": "0.0000", "XLIGHT Hub-Name": "XLIGHT Hub", "PIEZO-Description": "Ludl stage driver adapter", "PositionName": "Pos3", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSampleRate": "100000", "PRIME-FanSpeedSetpoint": "High", "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "405 AO-TriggerSampleRate": "100000", "470 Shutter-Block voltage": "false", "470-State": "0", "COM3-Parity": "None", "405 AO-Volts": "0.0000", "PRIME-TransposeMirrorY": "0", "PRIME-ChipName": "GS144BSI", "470 AO-Volts": "0.0000", "PRIME-TransposeMirrorX": "0", "445 Shutter-SupportsTriggering": "0", "470 Shutter-SupportsTriggering": "0", "XLIGHT Hub-Description": "XLIGHT hub", "PRIME-PreampOffLimit": "10000", "470 AO-Sequenceable": "1", "528 Shutter-MaxVolts": "5.0000", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "Core-XYStage": "XYStage", "470 AO-TriggerSampleRate": "100000", "PRIME-Timing-PreTriggerDelayNs": "24672000.0000", "528-DA Device": "528 Shutter", "Focus-Description": "Nikon TE2000 focus adapter", "FilterBlock-State": "5", "520 AO-Demo": "No", "COM3-DelayBetweenCharsMs": "0.0000", "470 Shutter-Volts": "0.0000", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "PRIME-PP 2 THRESHOLD": "125", "PRIME-PP 4 THRESHOLD": "75", "XYStage-Acceleration": "4.0000", "470 Shutter-Demo": "No", "PRIME-Name": "PMPCIECam00", "640-DA Device": "640 Shutter", "405 AO-Block voltage": "false", "ElapsedTime-ms": 132893, "PRIME-Offset": "100", "445 AO-IOChannel": "Dev1/ao1", "640 AO-MaxVolts": "5.0000", "PositionIndex": 3, "PRIME-PP 1 ENABLED": "Yes", "COM1-BaudRate": "9600", "640-State": "0", "COM3-Verbose": "1", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "LudlController-TransmissionDelay": "0", "PRIME-FullWellCapacity": "62000", "PRIME-SMARTStreamingEnabled": "No", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "640 Shutter-Description": "NI DAC", "528-Description": "DA device that is used as a shutter", "COM4-Description": "Serial port driver (boost:asio)", "405 Shutter-Description": "NI DAC", "TE2000-Name": "TE2000", "528 Shutter-Description": "NI DAC", "COM9-Description": "Serial port driver (boost:asio)", "LudlController-Name": "LudlController", "445 Shutter-TriggerSequenceLength": "1000", "PRIME-SerialNumber": "A18B203008", "445 AO-Volts": "0.0000", "PRIME-X-dimension": "1200", "PRIME-ShutterCloseDelay": "0", "XLIGHT Disk slider-State": "2", "Position": "Default", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 AO-IOChannel": "Dev1/ao5", "520 AO-IOChannel": "Dev1/ao4", "528 AO-TriggerSequenceLength": "1000", "405 Shutter-MaxVolts": "5.0000", "470-DA Device": "470 Shutter", "PRIME-FTCapable": "No", "Core-ChannelGroup": "Channels", "555 AO-SupportsTriggering": "0", "PRIME-Y-dimension": "1200", "Frame": 0, "555 Shutter-Sequenceable": "1", "PRIME-Timing-ExposureTimeNs": "9992000.0000", "Core-ImageProcessor": "", "528-State": "0", "520 AO-Block voltage": "false", "XLIGHT Excitation Wheel-Label": "CFP/YFP", "640 Shutter-Demo": "No", "COM3-StopBits": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "520 Shutter-IOChannel": "Dev1/ao11", "Width": 1200, "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-SupportsTriggering": "0", "640 AO-TriggerSampleRate": "100000", "520-Description": "DA device that is used as a shutter", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "405 Shutter-Sequenceable": "1", "405 Shutter-SupportsTriggering": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-TriggerSampleRate": "100000", "640 Shutter-MaxVolts": "5.0000", "555 AO-MaxVolts": "5.0000", "520 AO-TriggerSequenceLength": "1000", "COM3-BaudRate": "9600", "COM9-BaudRate": "9600", "Vincent-D3-Channel #": "Ch. #1", "PRIME-PMode": "Normal", "OpticalPath-Label": "Left(100)", "BitDepth": 16, "555 AO-TriggerSequenceLength": "1000", "445 Shutter-Description": "NI DAC", "405 AO-Sequenceable": "1", "PRIME-AcquisitionMethod": "Callbacks", "555 AO-Name": "AnalogIO", "PRIME-ShutterMode": "Pre-Exposure", "COM3-Handshaking": "Off", "445 AO-SupportsTriggering": "0", "ROI": "0-0-1200-1200", "COM9-Verbose": "1", "PRIME-Color": "OFF", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "PRIME-ShutterOpenDelay": "0", "COM1-Parity": "None", "Slice": 1, "SlicePosition": -40, "COM9-DelayBetweenCharsMs": "0.0000", "555-Name": "DA Shutter", "PIEZO-LudlSingleAxisName": "B", "XLIGHT Emission Wheel-Label": "565/24(G)", "640 Shutter-Name": "AnalogIO", "640 AO-Sequenceable": "1", "520 Shutter-Demo": "No", "528 Shutter-Demo": "No", "470-Name": "DA Shutter", "520-DA Device": "520 Shutter", "XYStage-Description": "Ludl XY stage driver adapter", "445 Shutter-MinVolts": "0.0000", "528 AO-TriggerSampleRate": "100000", "405 AO-MinVolts": "0.0000", "405 Shutter-TriggerSequenceLength": "1000", "Height": 1200, "555 AO-Block voltage": "false", "640 Shutter-Volts": "0.0000", "PixelType": "GRAY16", "445 Shutter-Block voltage": "false", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "520 Shutter-TriggerSequenceLength": "1000", "COM9-Name": "COM9", "Core-SLM": "", "555 AO-Volts": "0.0000", "555 Shutter-Block voltage": "false", "XYStage-Speed": "1800.0000", "520 AO-MaxVolts": "5.0000", "528 Shutter-SupportsTriggering": "0", "COM1-Description": "Serial port driver (boost:asio)", "PRIME-MetadataEnabled": "No", "520 Shutter-MinVolts": "0.0000", "470 Shutter-Sequenceable": "1", "PRIME-PreampDelay": "0", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-MinVolts": "0.0000", "ChannelIndex": 0, "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Core-AutoShutter": "1", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "Exposure-ms": 320, "PRIME-PP 2 ENABLED": "Yes", "640 Shutter-TriggerSampleRate": "100000", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "470 Shutter-IOChannel": "Dev1/ao10", "405 AO-Demo": "No", "PixelSizeUm": 1.1, "470 AO-Demo": "No", "470 Shutter-MaxVolts": "5.0000", "Vincent-D3-Name": "Vincent-D3", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FileName": "img_000000000_Il-G_001.tif", "LudlController-Reset": "Operate", "PRIME-Exposure": "320.00", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "445-Name": "DA Shutter", "555 Shutter-Name": "AnalogIO", "520 Shutter-TriggerSampleRate": "100000", "445 Shutter-IOChannel": "Dev1/ao9", "PRIME-TransposeCorrection": "0", "XYStage-StepSize": "0.1000", "445-Description": "DA device that is used as a shutter", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 AO-IOChannel": "Dev1/ao7", "520 Shutter-Name": "AnalogIO", "COM3-DataBits": "8", "640 AO-Volts": "0.0000", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "555 Shutter-IOChannel": "Dev1/ao13", "WaitInterval": null, "520 Shutter-Sequenceable": "1", "YPositionUm": 20690.300000000003, "445-State": "0", "445-DA Device": "445 Shutter", "PRIME-CircularBufferAutoSize": "ON", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "555 AO-TriggerSampleRate": "100000", "445 Shutter-Volts": "0.0000", "XLIGHT Hub-Port": "COM3", "Core-AutoFocus": "", "COM4-Handshaking": "Off", "528 Shutter-MinVolts": "0.0000", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "445 Shutter-Name": "AnalogIO", "528 Shutter-Sequenceable": "1", "XYStage-TransposeMirrorX": "0", "XYStage-TransposeMirrorY": "0", "PRIME-ReadoutRate": "100MHz 16bit", "XYStage-Name": "Stage", "640 AO-SupportsTriggering": "0", "640-Name": "DA Shutter", "640 Shutter-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "640 AO-Block voltage": "false", "445 Shutter-Sequenceable": "1", "470 AO-MaxVolts": "5.0000", "COM4-BaudRate": "9600", "470-Description": "DA device that is used as a shutter", "520 Shutter-Description": "NI DAC", "PRIME-CCDTemperature": "-14.9600", "Time": "2019-12-17 21:58:40 -0500", "640 AO-TriggerSequenceLength": "1000", "470 AO-Name": "AnalogIO", "XLIGHT Disk slider-Label": "Disk Pos 40um", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "Binning": "1x1", "TE2000-ModelType": "1", "COM3-AnswerTimeout": "500.0000", "555 Shutter-SupportsTriggering": "0", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "TE2000-Port": "COM4", "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Description": "NI DAC", "COM4-AnswerTimeout": "500.0000", "COM1-DelayBetweenCharsMs": "0.0000", "FilterBlock-Label": "Confocal", "445 AO-Name": "AnalogIO", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-Timing-ReadoutTimeNs": "24672000.0000", "528 AO-Demo": "No", "520 AO-Sequenceable": "1", "555-Description": "DA device that is used as a shutter", "405-DA Device": "405 Shutter", "405 AO-TriggerSequenceLength": "1000", "640 AO-MinVolts": "0.0000", "640 Shutter-Block voltage": "false", "FrameIndex": 0, "PRIME-Port": "CMOS", "555 AO-MinVolts": "0.0000", "COM3-Description": "Serial port driver (boost:asio)", "528 AO-Description": "NI DAC", "555 AO-IOChannel": "Dev1/ao6", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "555 AO-TriggerInputLine": "/Dev1/PFI0", "PRIME-ActualInterval-ms": "374.3700", "LudlController-Port": "COM9", "445 Shutter-Demo": "No", "PRIME-Trigger-Expose Out-Mux": "1", "SliceIndex": 1, "PRIME-CCDTemperatureSetPoint": "-15.0000", "405 Shutter-MinVolts": "0.0000", "PRIME-ExposeOutMode": "First Row", "AxisPositions": null, "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Volts": "0.0000", "528-Name": "DA Shutter", "555-State": "0", "XLIGHT Dichroic-Label": "CFP/YFP", "445 AO-Sequenceable": "1", "405-Name": "DA Shutter", "405 AO-IOChannel": "Dev1/ao0", "Core-Camera": "PRIME", "Focus-Name": "Focus", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "405 Shutter-IOChannel": "Dev1/ao8", "640 AO-Demo": "No", "640 Shutter-SupportsTriggering": "0", "445 AO-Demo": "No", "555-DA Device": "555 Shutter", "470 Shutter-Description": "NI DAC", "528 AO-Volts": "0.0000", "COM1-DataBits": "8", "Vincent-D3-Address": "x", "520 AO-SupportsTriggering": "0", "405-Description": "DA device that is used as a shutter", "640 Shutter-MinVolts": "0.0000", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "405 AO-Name": "AnalogIO", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "640 AO-TriggerInputLine": "/Dev1/PFI0", "405 AO-TriggerInputLine": "/Dev1/PFI0", "640-Description": "DA device that is used as a shutter", "445 Shutter-TriggerSampleRate": "100000", "470 Shutter-TriggerSequenceLength": "1000", "COM1-StopBits": "1", "520 Shutter-SupportsTriggering": "0", "405-State": "0", "Core-Initialize": "1", "XLIGHT Emission Wheel-State": "7", "520 AO-Description": "NI DAC", "528 AO-TriggerInputLine": "/Dev1/PFI0", "445 Shutter-MaxVolts": "5.0000", "COM9-Handshaking": "Off", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "XLIGHT Spin motor-Label": "On (spinning)", "PRIME-PP 5 ENABLED": "No", "UUID": "9fc6ac12-d3c1-4d26-ad8d-2e7ca26c2290", "PRIME-TriggerMode": "Internal Trigger", "COM1-Handshaking": "Off", "PRIME-Timing-PostTriggerDelayNs": "49344000.0000", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "Focus-Position": "16479.8000", "528 Shutter-Block voltage": "false", "Channel": "Il-G", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "555 Shutter-Demo": "No", "405 Shutter-Demo": "No", "528 AO-Name": "AnalogIO", "520-State": "1", "Core-Galvo": "", "405 Shutter-TriggerSampleRate": "100000", "Focus-FocusStepSize_(nm)": "100", "PRIME-PixelType": "16bit", "COM4-DataBits": "8", "470 AO-IOChannel": "Dev1/ao2", "520 AO-MinVolts": "0.0000", "COM9-Parity": "None", "PRIME-CircularBufferEnabled": "ON", "PRIME-PP 6 Reset": "No", "640 AO-Description": "NI DAC", "Source": "PRIME", "445 AO-MinVolts": "0.0000", "PIEZO-Name": "Stage", "445 AO-Description": "NI DAC", "PRIME-CircularBufferFrameCount": "46", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "520 AO-Volts": "4.5000", "528 Shutter-TriggerSampleRate": "100000", "XPositionUm": 62321, "OpticalPath-State": "4", "PRIME-PP 3 THRESHOLD": "80", "PRIME-PVCAM Version": "3.7.1", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Gain": "1-HDR", "640 Shutter-TriggerSequenceLength": "1000", "528 AO-SupportsTriggering": "0", "445 AO-TriggerInputLine": "/Dev1/PFI0", "OpticalPath-Name": "OpticalPath", "PRIME-PP 4 ENABLED": "Yes", "PRIME-PVCAM Adapter Version": "1.3.29", "COM3-Name": "COM3", "405 AO-MaxVolts": "5.0000", "520 Shutter-Block voltage": "false", "PRIME-Timing-ClearingTimeNs": "49344000.0000", "Core-Focus": "PIEZO", "528 AO-Block voltage": "false", "TE2000-Description": "Nikon TE2000 hub", "528 AO-MinVolts": "0.0000", "COM4-Parity": "None", "Summary": { "Slices": 11, "ChContrastMin": [ 0, 0, 0, 0, 0, 0 ], "PixelAspect": 1, "SlicesFirst": true, "Interval_ms": 0, "Time": "2019-12-17 21:58:39 -0500", "Prefix": "seq_3", "InitialPositionList": [ { "Label": "Pos0", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.435], "Focus": [16480.2], "XYStage": [ 69822.40000000001, 20100.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos1", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.425], "Focus": [16480.3], "XYStage": [ 69004, 20101 ] }, "GridColumnIndex": 0 }, { "Label": "Pos2", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.44], "Focus": [16480.1], "XYStage": [ 63119.5, 20690.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos3", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.405], "Focus": [16480.2], "XYStage": [ 62321, 20690.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos4", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.415], "Focus": [16480], "XYStage": [ 56604.100000000006, 20688.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos5", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.42000000000002], "Focus": [16480.1], "XYStage": [ 56034.200000000004, 20688.800000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos6", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.455], "Focus": [16480], "XYStage": [ 50889.3, 20496.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos7", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.43], "Focus": [16480.2], "XYStage": [ 50101.600000000006, 20496.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos8", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.67000000000002], "Focus": [16479.9], "XYStage": [ 44818.600000000006, 20496.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos9", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.67000000000002], "Focus": [16480], "XYStage": [ 43897.9, 20436.800000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos10", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.675], "Focus": [16479.9], "XYStage": [ 39670.600000000006, 20664.9 ] }, "GridColumnIndex": 0 }, { "Label": "Pos11", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.69], "Focus": [16479.9], "XYStage": [ 38734, 20664.600000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos12", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.695], "Focus": [16480.1], "XYStage": [ 34037.200000000004, 20664.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos13", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.685], "Focus": [16480.1], "XYStage": [ 33152, 20664.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos14", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.69], "Focus": [16480.1], "XYStage": [ 28122.800000000003, 20664.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos15", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.675], "Focus": [16480], "XYStage": [ 27422.9, 20664.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos16", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.64000000000001], "Focus": [16480], "XYStage": [ 23005.2, 20664.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos17", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.69], "Focus": [16479.9], "XYStage": [ 21820.600000000002, 20409.600000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos18", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.41], "Focus": [16480.1], "XYStage": [ 73697.6, -13222.800000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos19", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.455], "Focus": [16480.1], "XYStage": [ 72765.8, -13223 ] }, "GridColumnIndex": 0 }, { "Label": "Pos20", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.4], "Focus": [16480.2], "XYStage": [ 67445.5, -12303.800000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos21", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.415], "Focus": [16480.2], "XYStage": [ 66613.3, -12303.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos22", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.415], "Focus": [16480.1], "XYStage": [ 61819.4, -11936.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos23", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.405], "Focus": [16480.2], "XYStage": [ 61325.700000000004, -11937.1 ] }, "GridColumnIndex": 0 }, { "Label": "Pos24", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.46], "Focus": [16480.2], "XYStage": [ 55377, -10974.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos25", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.445], "Focus": [16480.1], "XYStage": [ 54827, -11214.800000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos26", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.435], "Focus": [16480.2], "XYStage": [ 49937.100000000006, -10919.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos27", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.425], "Focus": [16480.1], "XYStage": [ 48764, -10919.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos28", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.42000000000002], "Focus": [16480.2], "XYStage": [ 43978.4, -10920.400000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos29", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.435], "Focus": [16480.2], "XYStage": [ 43106.3, -10920.400000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos30", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.405], "Focus": [16480.3], "XYStage": [ 38212.4, -10920.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos31", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.445], "Focus": [16480.2], "XYStage": [ 37254.9, -10920.400000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos32", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.44], "Focus": [16480.3], "XYStage": [ 32472.800000000003, -11294.400000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos33", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [166.95000000000002], "Focus": [16480.2], "XYStage": [ 31420.7, -11294.400000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos34", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [166.95000000000002], "Focus": [16480.2], "XYStage": [ 26881.5, -11640 ] }, "GridColumnIndex": 0 }, { "Label": "Pos35", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [166.98], "Focus": [16480.2], "XYStage": [ 25876.100000000002, -11641.1 ] }, "GridColumnIndex": 0 } ], "z-step_um": 10, "GridRow": 0, "BitDepth": 16, "Depth": 2, "Channels": 6, "Source": "Micro-Manager", "ChColors": [ -16711681, -256, -65281, -1, -1, -16776961 ], "MetadataVersion": 10, "KeepShutterOpenChannels": true, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536, 65536 ], "CustomIntervals_ms": [], "Height": 1200, "MicroManagerVersion": "1.4.23 20180219", "GridColumn": 0, "UUID": "8c8f9d20-b128-4577-9f91-89e70f22d319", "PixelType": "GRAY16", "Width": 1200, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\Yan\\20191217-YC113-S5S6", "Frames": 1, "Comment": "", "UserName": "Nikon", "PixelSize_um": 1.1, "ROI": [ 0, 0, 1200, 1200 ], "Date": "2019-12-17", "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC", "P-DAPI-high" ], "KeepShutterOpenSlices": true, "IJType": 1, "ComputerName": "ZadorConfocal2", "Positions": 36, "PositionIndex": 3, "CameraTimeout": 20000, "TimeFirst": false }, "640 AO-Name": "AnalogIO", "Vincent-D3-Command": "Close" }, "FrameKey-0-0-2": { "COM4-StopBits": "1", "528 AO-MaxVolts": "5.0000", "XLIGHT Excitation Wheel-State": "2", "470 Shutter-Name": "AnalogIO", "Core-Shutter": "520", "555 AO-Demo": "No", "XLIGHT Dichroic-State": "2", "XYStage-StepSize-Y": "0.1000", "ZPositionUm": 147.405, "XYStage-StepSize-X": "0.1000", "PRIME-FirmwareVersion": "20.28", "PRIME-ClearCycles": "2", "520-Name": "DA Shutter", "405 Shutter-Name": "AnalogIO", "PIEZO-StepSize": "0.0050", "PRIME-ClearMode": "Pre-Exposure", "470 AO-MinVolts": "0.0000", "405 AO-SupportsTriggering": "1", "CameraChannelIndex": 0, "XYStage-StartSpeed": "500.0000", "COM9-StopBits": "2", "405 AO-Description": "NI DAC", "COM4-DelayBetweenCharsMs": "0.0000", "445 AO-Block voltage": "false", "520 AO-TriggerInputLine": "/Dev1/PFI0", "555 AO-Description": "NI DAC", "520 AO-TriggerSampleRate": "100000", "COM4-Verbose": "1", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM4-Name": "COM4", "405 Shutter-Volts": "0.0000", "Vincent-D3-Port": "COM1", "PRIME-PP 4 MIN ADU AFFECTED": "200", "470 Shutter-MinVolts": "0.0000", "PRIME-PP 3 ENABLED": "Yes", "470 AO-Description": "NI DAC", "PRIME-Description": "PVCAM API device adapter", "520 Shutter-MaxVolts": "5.0000", "520 Shutter-Volts": "5.0000", "528 AO-Sequenceable": "1", "PRIME-Binning": "1x1", "555 Shutter-MaxVolts": "5.0000", "528 Shutter-TriggerSequenceLength": "1000", "405 Shutter-Block voltage": "false", "XLIGHT Spin motor-State": "1", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "Vincent-D3-Time to open (ms)": "35.0000", "Vincent-D3-Time to close (ms)": "35.0000", "445 AO-TriggerSampleRate": "100000", "640 Shutter-IOChannel": "Dev1/ao14", "555 AO-Sequenceable": "1", "520 AO-Name": "AnalogIO", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "470 AO-Block voltage": "false", "COM1-Verbose": "1", "COM9-AnswerTimeout": "10000.0000", "528 Shutter-Name": "AnalogIO", "PRIME-Trigger Timeout (secs)": "10", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "NextFrame": 0, "470 AO-TriggerSequenceLength": "1000", "PRIME-TransposeXY": "0", "445 AO-TriggerSequenceLength": "1000", "COM1-Name": "COM1", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "COM9-DataBits": "8", "COM1-AnswerTimeout": "500.0000", "Camera": "", "TE2000-Version": "V2.50-V2.50-V2.10", "528 Shutter-Volts": "0.0000", "XLIGHT Hub-Name": "XLIGHT Hub", "PIEZO-Description": "Ludl stage driver adapter", "PositionName": "Pos3", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSampleRate": "100000", "PRIME-FanSpeedSetpoint": "High", "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "405 AO-TriggerSampleRate": "100000", "470 Shutter-Block voltage": "false", "470-State": "0", "COM3-Parity": "None", "405 AO-Volts": "0.0000", "PRIME-TransposeMirrorY": "0", "PRIME-ChipName": "GS144BSI", "470 AO-Volts": "0.0000", "PRIME-TransposeMirrorX": "0", "445 Shutter-SupportsTriggering": "0", "470 Shutter-SupportsTriggering": "0", "XLIGHT Hub-Description": "XLIGHT hub", "PRIME-PreampOffLimit": "10000", "470 AO-Sequenceable": "1", "528 Shutter-MaxVolts": "5.0000", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "Core-XYStage": "XYStage", "470 AO-TriggerSampleRate": "100000", "PRIME-Timing-PreTriggerDelayNs": "24672000.0000", "528-DA Device": "528 Shutter", "Focus-Description": "Nikon TE2000 focus adapter", "FilterBlock-State": "5", "520 AO-Demo": "No", "COM3-DelayBetweenCharsMs": "0.0000", "470 Shutter-Volts": "0.0000", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "PRIME-PP 2 THRESHOLD": "125", "PRIME-PP 4 THRESHOLD": "75", "XYStage-Acceleration": "4.0000", "470 Shutter-Demo": "No", "PRIME-Name": "PMPCIECam00", "640-DA Device": "640 Shutter", "405 AO-Block voltage": "false", "ElapsedTime-ms": 133452, "PRIME-Offset": "100", "445 AO-IOChannel": "Dev1/ao1", "640 AO-MaxVolts": "5.0000", "PositionIndex": 3, "PRIME-PP 1 ENABLED": "Yes", "COM1-BaudRate": "9600", "640-State": "0", "COM3-Verbose": "1", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "LudlController-TransmissionDelay": "0", "PRIME-FullWellCapacity": "62000", "PRIME-SMARTStreamingEnabled": "No", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "640 Shutter-Description": "NI DAC", "528-Description": "DA device that is used as a shutter", "COM4-Description": "Serial port driver (boost:asio)", "405 Shutter-Description": "NI DAC", "TE2000-Name": "TE2000", "528 Shutter-Description": "NI DAC", "COM9-Description": "Serial port driver (boost:asio)", "LudlController-Name": "LudlController", "445 Shutter-TriggerSequenceLength": "1000", "PRIME-SerialNumber": "A18B203008", "445 AO-Volts": "0.0000", "PRIME-X-dimension": "1200", "PRIME-ShutterCloseDelay": "0", "XLIGHT Disk slider-State": "2", "Position": "Default", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 AO-IOChannel": "Dev1/ao5", "520 AO-IOChannel": "Dev1/ao4", "528 AO-TriggerSequenceLength": "1000", "405 Shutter-MaxVolts": "5.0000", "470-DA Device": "470 Shutter", "PRIME-FTCapable": "No", "Core-ChannelGroup": "Channels", "555 AO-SupportsTriggering": "0", "PRIME-Y-dimension": "1200", "Frame": 0, "555 Shutter-Sequenceable": "1", "PRIME-Timing-ExposureTimeNs": "9992000.0000", "Core-ImageProcessor": "", "528-State": "0", "520 AO-Block voltage": "false", "XLIGHT Excitation Wheel-Label": "CFP/YFP", "640 Shutter-Demo": "No", "COM3-StopBits": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "520 Shutter-IOChannel": "Dev1/ao11", "Width": 1200, "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-SupportsTriggering": "0", "640 AO-TriggerSampleRate": "100000", "520-Description": "DA device that is used as a shutter", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "405 Shutter-Sequenceable": "1", "405 Shutter-SupportsTriggering": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-TriggerSampleRate": "100000", "640 Shutter-MaxVolts": "5.0000", "555 AO-MaxVolts": "5.0000", "520 AO-TriggerSequenceLength": "1000", "COM3-BaudRate": "9600", "COM9-BaudRate": "9600", "Vincent-D3-Channel #": "Ch. #1", "PRIME-PMode": "Normal", "OpticalPath-Label": "Left(100)", "BitDepth": 16, "555 AO-TriggerSequenceLength": "1000", "445 Shutter-Description": "NI DAC", "405 AO-Sequenceable": "1", "PRIME-AcquisitionMethod": "Callbacks", "555 AO-Name": "AnalogIO", "PRIME-ShutterMode": "Pre-Exposure", "COM3-Handshaking": "Off", "445 AO-SupportsTriggering": "0", "ROI": "0-0-1200-1200", "COM9-Verbose": "1", "PRIME-Color": "OFF", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "PRIME-ShutterOpenDelay": "0", "COM1-Parity": "None", "Slice": 2, "SlicePosition": -30, "COM9-DelayBetweenCharsMs": "0.0000", "555-Name": "DA Shutter", "PIEZO-LudlSingleAxisName": "B", "XLIGHT Emission Wheel-Label": "565/24(G)", "640 Shutter-Name": "AnalogIO", "640 AO-Sequenceable": "1", "520 Shutter-Demo": "No", "528 Shutter-Demo": "No", "470-Name": "DA Shutter", "520-DA Device": "520 Shutter", "XYStage-Description": "Ludl XY stage driver adapter", "445 Shutter-MinVolts": "0.0000", "528 AO-TriggerSampleRate": "100000", "405 AO-MinVolts": "0.0000", "405 Shutter-TriggerSequenceLength": "1000", "Height": 1200, "555 AO-Block voltage": "false", "640 Shutter-Volts": "0.0000", "PixelType": "GRAY16", "445 Shutter-Block voltage": "false", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "520 Shutter-TriggerSequenceLength": "1000", "COM9-Name": "COM9", "Core-SLM": "", "555 AO-Volts": "0.0000", "555 Shutter-Block voltage": "false", "XYStage-Speed": "1800.0000", "520 AO-MaxVolts": "5.0000", "528 Shutter-SupportsTriggering": "0", "COM1-Description": "Serial port driver (boost:asio)", "PRIME-MetadataEnabled": "No", "520 Shutter-MinVolts": "0.0000", "470 Shutter-Sequenceable": "1", "PRIME-PreampDelay": "0", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-MinVolts": "0.0000", "ChannelIndex": 0, "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Core-AutoShutter": "1", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "Exposure-ms": 320, "PRIME-PP 2 ENABLED": "Yes", "640 Shutter-TriggerSampleRate": "100000", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "470 Shutter-IOChannel": "Dev1/ao10", "405 AO-Demo": "No", "PixelSizeUm": 1.1, "470 AO-Demo": "No", "470 Shutter-MaxVolts": "5.0000", "Vincent-D3-Name": "Vincent-D3", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FileName": "img_000000000_Il-G_002.tif", "LudlController-Reset": "Operate", "PRIME-Exposure": "320.00", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "445-Name": "DA Shutter", "555 Shutter-Name": "AnalogIO", "520 Shutter-TriggerSampleRate": "100000", "445 Shutter-IOChannel": "Dev1/ao9", "PRIME-TransposeCorrection": "0", "XYStage-StepSize": "0.1000", "445-Description": "DA device that is used as a shutter", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 AO-IOChannel": "Dev1/ao7", "520 Shutter-Name": "AnalogIO", "COM3-DataBits": "8", "640 AO-Volts": "0.0000", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "555 Shutter-IOChannel": "Dev1/ao13", "WaitInterval": null, "520 Shutter-Sequenceable": "1", "YPositionUm": 20690.300000000003, "445-State": "0", "445-DA Device": "445 Shutter", "PRIME-CircularBufferAutoSize": "ON", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "555 AO-TriggerSampleRate": "100000", "445 Shutter-Volts": "0.0000", "XLIGHT Hub-Port": "COM3", "Core-AutoFocus": "", "COM4-Handshaking": "Off", "528 Shutter-MinVolts": "0.0000", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "445 Shutter-Name": "AnalogIO", "528 Shutter-Sequenceable": "1", "XYStage-TransposeMirrorX": "0", "XYStage-TransposeMirrorY": "0", "PRIME-ReadoutRate": "100MHz 16bit", "XYStage-Name": "Stage", "640 AO-SupportsTriggering": "0", "640-Name": "DA Shutter", "640 Shutter-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "640 AO-Block voltage": "false", "445 Shutter-Sequenceable": "1", "470 AO-MaxVolts": "5.0000", "COM4-BaudRate": "9600", "470-Description": "DA device that is used as a shutter", "520 Shutter-Description": "NI DAC", "PRIME-CCDTemperature": "-14.9600", "Time": "2019-12-17 21:58:40 -0500", "640 AO-TriggerSequenceLength": "1000", "470 AO-Name": "AnalogIO", "XLIGHT Disk slider-Label": "Disk Pos 40um", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "Binning": "1x1", "TE2000-ModelType": "1", "COM3-AnswerTimeout": "500.0000", "555 Shutter-SupportsTriggering": "0", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "TE2000-Port": "COM4", "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Description": "NI DAC", "COM4-AnswerTimeout": "500.0000", "COM1-DelayBetweenCharsMs": "0.0000", "FilterBlock-Label": "Confocal", "445 AO-Name": "AnalogIO", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-Timing-ReadoutTimeNs": "24672000.0000", "528 AO-Demo": "No", "520 AO-Sequenceable": "1", "555-Description": "DA device that is used as a shutter", "405-DA Device": "405 Shutter", "405 AO-TriggerSequenceLength": "1000", "640 AO-MinVolts": "0.0000", "640 Shutter-Block voltage": "false", "FrameIndex": 0, "PRIME-Port": "CMOS", "555 AO-MinVolts": "0.0000", "COM3-Description": "Serial port driver (boost:asio)", "528 AO-Description": "NI DAC", "555 AO-IOChannel": "Dev1/ao6", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "555 AO-TriggerInputLine": "/Dev1/PFI0", "PRIME-ActualInterval-ms": "374.3700", "LudlController-Port": "COM9", "445 Shutter-Demo": "No", "PRIME-Trigger-Expose Out-Mux": "1", "SliceIndex": 2, "PRIME-CCDTemperatureSetPoint": "-15.0000", "405 Shutter-MinVolts": "0.0000", "PRIME-ExposeOutMode": "First Row", "AxisPositions": null, "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Volts": "0.0000", "528-Name": "DA Shutter", "555-State": "0", "XLIGHT Dichroic-Label": "CFP/YFP", "445 AO-Sequenceable": "1", "405-Name": "DA Shutter", "405 AO-IOChannel": "Dev1/ao0", "Core-Camera": "PRIME", "Focus-Name": "Focus", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "405 Shutter-IOChannel": "Dev1/ao8", "640 AO-Demo": "No", "640 Shutter-SupportsTriggering": "0", "445 AO-Demo": "No", "555-DA Device": "555 Shutter", "470 Shutter-Description": "NI DAC", "528 AO-Volts": "0.0000", "COM1-DataBits": "8", "Vincent-D3-Address": "x", "520 AO-SupportsTriggering": "0", "405-Description": "DA device that is used as a shutter", "640 Shutter-MinVolts": "0.0000", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "405 AO-Name": "AnalogIO", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "640 AO-TriggerInputLine": "/Dev1/PFI0", "405 AO-TriggerInputLine": "/Dev1/PFI0", "640-Description": "DA device that is used as a shutter", "445 Shutter-TriggerSampleRate": "100000", "470 Shutter-TriggerSequenceLength": "1000", "COM1-StopBits": "1", "520 Shutter-SupportsTriggering": "0", "405-State": "0", "Core-Initialize": "1", "XLIGHT Emission Wheel-State": "7", "520 AO-Description": "NI DAC", "528 AO-TriggerInputLine": "/Dev1/PFI0", "445 Shutter-MaxVolts": "5.0000", "COM9-Handshaking": "Off", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "XLIGHT Spin motor-Label": "On (spinning)", "PRIME-PP 5 ENABLED": "No", "UUID": "2d7065ba-2b76-4dcf-9c75-898ccbfc4cf1", "PRIME-TriggerMode": "Internal Trigger", "COM1-Handshaking": "Off", "PRIME-Timing-PostTriggerDelayNs": "49344000.0000", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "Focus-Position": "16479.8000", "528 Shutter-Block voltage": "false", "Channel": "Il-G", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "555 Shutter-Demo": "No", "405 Shutter-Demo": "No", "528 AO-Name": "AnalogIO", "520-State": "1", "Core-Galvo": "", "405 Shutter-TriggerSampleRate": "100000", "Focus-FocusStepSize_(nm)": "100", "PRIME-PixelType": "16bit", "COM4-DataBits": "8", "470 AO-IOChannel": "Dev1/ao2", "520 AO-MinVolts": "0.0000", "COM9-Parity": "None", "PRIME-CircularBufferEnabled": "ON", "PRIME-PP 6 Reset": "No", "640 AO-Description": "NI DAC", "Source": "PRIME", "445 AO-MinVolts": "0.0000", "PIEZO-Name": "Stage", "445 AO-Description": "NI DAC", "PRIME-CircularBufferFrameCount": "46", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "520 AO-Volts": "4.5000", "528 Shutter-TriggerSampleRate": "100000", "XPositionUm": 62321, "OpticalPath-State": "4", "PRIME-PP 3 THRESHOLD": "80", "PRIME-PVCAM Version": "3.7.1", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Gain": "1-HDR", "640 Shutter-TriggerSequenceLength": "1000", "528 AO-SupportsTriggering": "0", "445 AO-TriggerInputLine": "/Dev1/PFI0", "OpticalPath-Name": "OpticalPath", "PRIME-PP 4 ENABLED": "Yes", "PRIME-PVCAM Adapter Version": "1.3.29", "COM3-Name": "COM3", "405 AO-MaxVolts": "5.0000", "520 Shutter-Block voltage": "false", "PRIME-Timing-ClearingTimeNs": "49344000.0000", "Core-Focus": "PIEZO", "528 AO-Block voltage": "false", "TE2000-Description": "Nikon TE2000 hub", "528 AO-MinVolts": "0.0000", "COM4-Parity": "None", "Summary": { "Slices": 11, "ChContrastMin": [ 0, 0, 0, 0, 0, 0 ], "PixelAspect": 1, "SlicesFirst": true, "Interval_ms": 0, "Time": "2019-12-17 21:58:39 -0500", "Prefix": "seq_3", "InitialPositionList": [ { "Label": "Pos0", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.435], "Focus": [16480.2], "XYStage": [ 69822.40000000001, 20100.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos1", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.425], "Focus": [16480.3], "XYStage": [ 69004, 20101 ] }, "GridColumnIndex": 0 }, { "Label": "Pos2", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.44], "Focus": [16480.1], "XYStage": [ 63119.5, 20690.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos3", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.405], "Focus": [16480.2], "XYStage": [ 62321, 20690.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos4", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.415], "Focus": [16480], "XYStage": [ 56604.100000000006, 20688.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos5", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.42000000000002], "Focus": [16480.1], "XYStage": [ 56034.200000000004, 20688.800000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos6", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.455], "Focus": [16480], "XYStage": [ 50889.3, 20496.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos7", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.43], "Focus": [16480.2], "XYStage": [ 50101.600000000006, 20496.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos8", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.67000000000002], "Focus": [16479.9], "XYStage": [ 44818.600000000006, 20496.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos9", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.67000000000002], "Focus": [16480], "XYStage": [ 43897.9, 20436.800000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos10", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.675], "Focus": [16479.9], "XYStage": [ 39670.600000000006, 20664.9 ] }, "GridColumnIndex": 0 }, { "Label": "Pos11", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.69], "Focus": [16479.9], "XYStage": [ 38734, 20664.600000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos12", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.695], "Focus": [16480.1], "XYStage": [ 34037.200000000004, 20664.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos13", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.685], "Focus": [16480.1], "XYStage": [ 33152, 20664.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos14", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.69], "Focus": [16480.1], "XYStage": [ 28122.800000000003, 20664.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos15", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.675], "Focus": [16480], "XYStage": [ 27422.9, 20664.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos16", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.64000000000001], "Focus": [16480], "XYStage": [ 23005.2, 20664.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos17", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.69], "Focus": [16479.9], "XYStage": [ 21820.600000000002, 20409.600000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos18", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.41], "Focus": [16480.1], "XYStage": [ 73697.6, -13222.800000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos19", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.455], "Focus": [16480.1], "XYStage": [ 72765.8, -13223 ] }, "GridColumnIndex": 0 }, { "Label": "Pos20", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.4], "Focus": [16480.2], "XYStage": [ 67445.5, -12303.800000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos21", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.415], "Focus": [16480.2], "XYStage": [ 66613.3, -12303.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos22", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.415], "Focus": [16480.1], "XYStage": [ 61819.4, -11936.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos23", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.405], "Focus": [16480.2], "XYStage": [ 61325.700000000004, -11937.1 ] }, "GridColumnIndex": 0 }, { "Label": "Pos24", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.46], "Focus": [16480.2], "XYStage": [ 55377, -10974.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos25", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.445], "Focus": [16480.1], "XYStage": [ 54827, -11214.800000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos26", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.435], "Focus": [16480.2], "XYStage": [ 49937.100000000006, -10919.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos27", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.425], "Focus": [16480.1], "XYStage": [ 48764, -10919.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos28", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.42000000000002], "Focus": [16480.2], "XYStage": [ 43978.4, -10920.400000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos29", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.435], "Focus": [16480.2], "XYStage": [ 43106.3, -10920.400000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos30", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.405], "Focus": [16480.3], "XYStage": [ 38212.4, -10920.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos31", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.445], "Focus": [16480.2], "XYStage": [ 37254.9, -10920.400000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos32", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.44], "Focus": [16480.3], "XYStage": [ 32472.800000000003, -11294.400000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos33", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [166.95000000000002], "Focus": [16480.2], "XYStage": [ 31420.7, -11294.400000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos34", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [166.95000000000002], "Focus": [16480.2], "XYStage": [ 26881.5, -11640 ] }, "GridColumnIndex": 0 }, { "Label": "Pos35", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [166.98], "Focus": [16480.2], "XYStage": [ 25876.100000000002, -11641.1 ] }, "GridColumnIndex": 0 } ], "z-step_um": 10, "GridRow": 0, "BitDepth": 16, "Depth": 2, "Channels": 6, "Source": "Micro-Manager", "ChColors": [ -16711681, -256, -65281, -1, -1, -16776961 ], "MetadataVersion": 10, "KeepShutterOpenChannels": true, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536, 65536 ], "CustomIntervals_ms": [], "Height": 1200, "MicroManagerVersion": "1.4.23 20180219", "GridColumn": 0, "UUID": "8c8f9d20-b128-4577-9f91-89e70f22d319", "PixelType": "GRAY16", "Width": 1200, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\Yan\\20191217-YC113-S5S6", "Frames": 1, "Comment": "", "UserName": "Nikon", "PixelSize_um": 1.1, "ROI": [ 0, 0, 1200, 1200 ], "Date": "2019-12-17", "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC", "P-DAPI-high" ], "KeepShutterOpenSlices": true, "IJType": 1, "ComputerName": "ZadorConfocal2", "Positions": 36, "PositionIndex": 3, "CameraTimeout": 20000, "TimeFirst": false }, "640 AO-Name": "AnalogIO", "Vincent-D3-Command": "Close" }, "FrameKey-0-0-3": { "COM4-StopBits": "1", "528 AO-MaxVolts": "5.0000", "XLIGHT Excitation Wheel-State": "2", "470 Shutter-Name": "AnalogIO", "Core-Shutter": "520", "555 AO-Demo": "No", "XLIGHT Dichroic-State": "2", "XYStage-StepSize-Y": "0.1000", "ZPositionUm": 157.405, "XYStage-StepSize-X": "0.1000", "PRIME-FirmwareVersion": "20.28", "PRIME-ClearCycles": "2", "520-Name": "DA Shutter", "405 Shutter-Name": "AnalogIO", "PIEZO-StepSize": "0.0050", "PRIME-ClearMode": "Pre-Exposure", "470 AO-MinVolts": "0.0000", "405 AO-SupportsTriggering": "1", "CameraChannelIndex": 0, "XYStage-StartSpeed": "500.0000", "COM9-StopBits": "2", "405 AO-Description": "NI DAC", "COM4-DelayBetweenCharsMs": "0.0000", "445 AO-Block voltage": "false", "520 AO-TriggerInputLine": "/Dev1/PFI0", "555 AO-Description": "NI DAC", "520 AO-TriggerSampleRate": "100000", "COM4-Verbose": "1", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM4-Name": "COM4", "405 Shutter-Volts": "0.0000", "Vincent-D3-Port": "COM1", "PRIME-PP 4 MIN ADU AFFECTED": "200", "470 Shutter-MinVolts": "0.0000", "PRIME-PP 3 ENABLED": "Yes", "470 AO-Description": "NI DAC", "PRIME-Description": "PVCAM API device adapter", "520 Shutter-MaxVolts": "5.0000", "520 Shutter-Volts": "5.0000", "528 AO-Sequenceable": "1", "PRIME-Binning": "1x1", "555 Shutter-MaxVolts": "5.0000", "528 Shutter-TriggerSequenceLength": "1000", "405 Shutter-Block voltage": "false", "XLIGHT Spin motor-State": "1", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "Vincent-D3-Time to open (ms)": "35.0000", "Vincent-D3-Time to close (ms)": "35.0000", "445 AO-TriggerSampleRate": "100000", "640 Shutter-IOChannel": "Dev1/ao14", "555 AO-Sequenceable": "1", "520 AO-Name": "AnalogIO", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "470 AO-Block voltage": "false", "COM1-Verbose": "1", "COM9-AnswerTimeout": "10000.0000", "528 Shutter-Name": "AnalogIO", "PRIME-Trigger Timeout (secs)": "10", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "NextFrame": 0, "470 AO-TriggerSequenceLength": "1000", "PRIME-TransposeXY": "0", "445 AO-TriggerSequenceLength": "1000", "COM1-Name": "COM1", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "COM9-DataBits": "8", "COM1-AnswerTimeout": "500.0000", "Camera": "", "TE2000-Version": "V2.50-V2.50-V2.10", "528 Shutter-Volts": "0.0000", "XLIGHT Hub-Name": "XLIGHT Hub", "PIEZO-Description": "Ludl stage driver adapter", "PositionName": "Pos3", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSampleRate": "100000", "PRIME-FanSpeedSetpoint": "High", "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "405 AO-TriggerSampleRate": "100000", "470 Shutter-Block voltage": "false", "470-State": "0", "COM3-Parity": "None", "405 AO-Volts": "0.0000", "PRIME-TransposeMirrorY": "0", "PRIME-ChipName": "GS144BSI", "470 AO-Volts": "0.0000", "PRIME-TransposeMirrorX": "0", "445 Shutter-SupportsTriggering": "0", "470 Shutter-SupportsTriggering": "0", "XLIGHT Hub-Description": "XLIGHT hub", "PRIME-PreampOffLimit": "10000", "470 AO-Sequenceable": "1", "528 Shutter-MaxVolts": "5.0000", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "Core-XYStage": "XYStage", "470 AO-TriggerSampleRate": "100000", "PRIME-Timing-PreTriggerDelayNs": "24672000.0000", "528-DA Device": "528 Shutter", "Focus-Description": "Nikon TE2000 focus adapter", "FilterBlock-State": "5", "520 AO-Demo": "No", "COM3-DelayBetweenCharsMs": "0.0000", "470 Shutter-Volts": "0.0000", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "PRIME-PP 2 THRESHOLD": "125", "PRIME-PP 4 THRESHOLD": "75", "XYStage-Acceleration": "4.0000", "470 Shutter-Demo": "No", "PRIME-Name": "PMPCIECam00", "640-DA Device": "640 Shutter", "405 AO-Block voltage": "false", "ElapsedTime-ms": 134022, "PRIME-Offset": "100", "445 AO-IOChannel": "Dev1/ao1", "640 AO-MaxVolts": "5.0000", "PositionIndex": 3, "PRIME-PP 1 ENABLED": "Yes", "COM1-BaudRate": "9600", "640-State": "0", "COM3-Verbose": "1", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "LudlController-TransmissionDelay": "0", "PRIME-FullWellCapacity": "62000", "PRIME-SMARTStreamingEnabled": "No", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "640 Shutter-Description": "NI DAC", "528-Description": "DA device that is used as a shutter", "COM4-Description": "Serial port driver (boost:asio)", "405 Shutter-Description": "NI DAC", "TE2000-Name": "TE2000", "528 Shutter-Description": "NI DAC", "COM9-Description": "Serial port driver (boost:asio)", "LudlController-Name": "LudlController", "445 Shutter-TriggerSequenceLength": "1000", "PRIME-SerialNumber": "A18B203008", "445 AO-Volts": "0.0000", "PRIME-X-dimension": "1200", "PRIME-ShutterCloseDelay": "0", "XLIGHT Disk slider-State": "2", "Position": "Default", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 AO-IOChannel": "Dev1/ao5", "520 AO-IOChannel": "Dev1/ao4", "528 AO-TriggerSequenceLength": "1000", "405 Shutter-MaxVolts": "5.0000", "470-DA Device": "470 Shutter", "PRIME-FTCapable": "No", "Core-ChannelGroup": "Channels", "555 AO-SupportsTriggering": "0", "PRIME-Y-dimension": "1200", "Frame": 0, "555 Shutter-Sequenceable": "1", "PRIME-Timing-ExposureTimeNs": "9992000.0000", "Core-ImageProcessor": "", "528-State": "0", "520 AO-Block voltage": "false", "XLIGHT Excitation Wheel-Label": "CFP/YFP", "640 Shutter-Demo": "No", "COM3-StopBits": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "520 Shutter-IOChannel": "Dev1/ao11", "Width": 1200, "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-SupportsTriggering": "0", "640 AO-TriggerSampleRate": "100000", "520-Description": "DA device that is used as a shutter", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "405 Shutter-Sequenceable": "1", "405 Shutter-SupportsTriggering": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-TriggerSampleRate": "100000", "640 Shutter-MaxVolts": "5.0000", "555 AO-MaxVolts": "5.0000", "520 AO-TriggerSequenceLength": "1000", "COM3-BaudRate": "9600", "COM9-BaudRate": "9600", "Vincent-D3-Channel #": "Ch. #1", "PRIME-PMode": "Normal", "OpticalPath-Label": "Left(100)", "BitDepth": 16, "555 AO-TriggerSequenceLength": "1000", "445 Shutter-Description": "NI DAC", "405 AO-Sequenceable": "1", "PRIME-AcquisitionMethod": "Callbacks", "555 AO-Name": "AnalogIO", "PRIME-ShutterMode": "Pre-Exposure", "COM3-Handshaking": "Off", "445 AO-SupportsTriggering": "0", "ROI": "0-0-1200-1200", "COM9-Verbose": "1", "PRIME-Color": "OFF", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "PRIME-ShutterOpenDelay": "0", "COM1-Parity": "None", "Slice": 3, "SlicePosition": -20, "COM9-DelayBetweenCharsMs": "0.0000", "555-Name": "DA Shutter", "PIEZO-LudlSingleAxisName": "B", "XLIGHT Emission Wheel-Label": "565/24(G)", "640 Shutter-Name": "AnalogIO", "640 AO-Sequenceable": "1", "520 Shutter-Demo": "No", "528 Shutter-Demo": "No", "470-Name": "DA Shutter", "520-DA Device": "520 Shutter", "XYStage-Description": "Ludl XY stage driver adapter", "445 Shutter-MinVolts": "0.0000", "528 AO-TriggerSampleRate": "100000", "405 AO-MinVolts": "0.0000", "405 Shutter-TriggerSequenceLength": "1000", "Height": 1200, "555 AO-Block voltage": "false", "640 Shutter-Volts": "0.0000", "PixelType": "GRAY16", "445 Shutter-Block voltage": "false", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "520 Shutter-TriggerSequenceLength": "1000", "COM9-Name": "COM9", "Core-SLM": "", "555 AO-Volts": "0.0000", "555 Shutter-Block voltage": "false", "XYStage-Speed": "1800.0000", "520 AO-MaxVolts": "5.0000", "528 Shutter-SupportsTriggering": "0", "COM1-Description": "Serial port driver (boost:asio)", "PRIME-MetadataEnabled": "No", "520 Shutter-MinVolts": "0.0000", "470 Shutter-Sequenceable": "1", "PRIME-PreampDelay": "0", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-MinVolts": "0.0000", "ChannelIndex": 0, "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Core-AutoShutter": "1", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "Exposure-ms": 320, "PRIME-PP 2 ENABLED": "Yes", "640 Shutter-TriggerSampleRate": "100000", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "470 Shutter-IOChannel": "Dev1/ao10", "405 AO-Demo": "No", "PixelSizeUm": 1.1, "470 AO-Demo": "No", "470 Shutter-MaxVolts": "5.0000", "Vincent-D3-Name": "Vincent-D3", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FileName": "img_000000000_Il-G_003.tif", "LudlController-Reset": "Operate", "PRIME-Exposure": "320.00", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "445-Name": "DA Shutter", "555 Shutter-Name": "AnalogIO", "520 Shutter-TriggerSampleRate": "100000", "445 Shutter-IOChannel": "Dev1/ao9", "PRIME-TransposeCorrection": "0", "XYStage-StepSize": "0.1000", "445-Description": "DA device that is used as a shutter", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 AO-IOChannel": "Dev1/ao7", "520 Shutter-Name": "AnalogIO", "COM3-DataBits": "8", "640 AO-Volts": "0.0000", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "555 Shutter-IOChannel": "Dev1/ao13", "WaitInterval": null, "520 Shutter-Sequenceable": "1", "YPositionUm": 20690.300000000003, "445-State": "0", "445-DA Device": "445 Shutter", "PRIME-CircularBufferAutoSize": "ON", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "555 AO-TriggerSampleRate": "100000", "445 Shutter-Volts": "0.0000", "XLIGHT Hub-Port": "COM3", "Core-AutoFocus": "", "COM4-Handshaking": "Off", "528 Shutter-MinVolts": "0.0000", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "445 Shutter-Name": "AnalogIO", "528 Shutter-Sequenceable": "1", "XYStage-TransposeMirrorX": "0", "XYStage-TransposeMirrorY": "0", "PRIME-ReadoutRate": "100MHz 16bit", "XYStage-Name": "Stage", "640 AO-SupportsTriggering": "0", "640-Name": "DA Shutter", "640 Shutter-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "640 AO-Block voltage": "false", "445 Shutter-Sequenceable": "1", "470 AO-MaxVolts": "5.0000", "COM4-BaudRate": "9600", "470-Description": "DA device that is used as a shutter", "520 Shutter-Description": "NI DAC", "PRIME-CCDTemperature": "-14.9600", "Time": "2019-12-17 21:58:41 -0500", "640 AO-TriggerSequenceLength": "1000", "470 AO-Name": "AnalogIO", "XLIGHT Disk slider-Label": "Disk Pos 40um", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "Binning": "1x1", "TE2000-ModelType": "1", "COM3-AnswerTimeout": "500.0000", "555 Shutter-SupportsTriggering": "0", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "TE2000-Port": "COM4", "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Description": "NI DAC", "COM4-AnswerTimeout": "500.0000", "COM1-DelayBetweenCharsMs": "0.0000", "FilterBlock-Label": "Confocal", "445 AO-Name": "AnalogIO", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-Timing-ReadoutTimeNs": "24672000.0000", "528 AO-Demo": "No", "520 AO-Sequenceable": "1", "555-Description": "DA device that is used as a shutter", "405-DA Device": "405 Shutter", "405 AO-TriggerSequenceLength": "1000", "640 AO-MinVolts": "0.0000", "640 Shutter-Block voltage": "false", "FrameIndex": 0, "PRIME-Port": "CMOS", "555 AO-MinVolts": "0.0000", "COM3-Description": "Serial port driver (boost:asio)", "528 AO-Description": "NI DAC", "555 AO-IOChannel": "Dev1/ao6", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "555 AO-TriggerInputLine": "/Dev1/PFI0", "PRIME-ActualInterval-ms": "374.3700", "LudlController-Port": "COM9", "445 Shutter-Demo": "No", "PRIME-Trigger-Expose Out-Mux": "1", "SliceIndex": 3, "PRIME-CCDTemperatureSetPoint": "-15.0000", "405 Shutter-MinVolts": "0.0000", "PRIME-ExposeOutMode": "First Row", "AxisPositions": null, "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Volts": "0.0000", "528-Name": "DA Shutter", "555-State": "0", "XLIGHT Dichroic-Label": "CFP/YFP", "445 AO-Sequenceable": "1", "405-Name": "DA Shutter", "405 AO-IOChannel": "Dev1/ao0", "Core-Camera": "PRIME", "Focus-Name": "Focus", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "405 Shutter-IOChannel": "Dev1/ao8", "640 AO-Demo": "No", "640 Shutter-SupportsTriggering": "0", "445 AO-Demo": "No", "555-DA Device": "555 Shutter", "470 Shutter-Description": "NI DAC", "528 AO-Volts": "0.0000", "COM1-DataBits": "8", "Vincent-D3-Address": "x", "520 AO-SupportsTriggering": "0", "405-Description": "DA device that is used as a shutter", "640 Shutter-MinVolts": "0.0000", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "405 AO-Name": "AnalogIO", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "640 AO-TriggerInputLine": "/Dev1/PFI0", "405 AO-TriggerInputLine": "/Dev1/PFI0", "640-Description": "DA device that is used as a shutter", "445 Shutter-TriggerSampleRate": "100000", "470 Shutter-TriggerSequenceLength": "1000", "COM1-StopBits": "1", "520 Shutter-SupportsTriggering": "0", "405-State": "0", "Core-Initialize": "1", "XLIGHT Emission Wheel-State": "7", "520 AO-Description": "NI DAC", "528 AO-TriggerInputLine": "/Dev1/PFI0", "445 Shutter-MaxVolts": "5.0000", "COM9-Handshaking": "Off", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "XLIGHT Spin motor-Label": "On (spinning)", "PRIME-PP 5 ENABLED": "No", "UUID": "43049793-82c3-4ce9-bfc2-ddd1c8e53200", "PRIME-TriggerMode": "Internal Trigger", "COM1-Handshaking": "Off", "PRIME-Timing-PostTriggerDelayNs": "49344000.0000", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "Focus-Position": "16479.8000", "528 Shutter-Block voltage": "false", "Channel": "Il-G", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "555 Shutter-Demo": "No", "405 Shutter-Demo": "No", "528 AO-Name": "AnalogIO", "520-State": "1", "Core-Galvo": "", "405 Shutter-TriggerSampleRate": "100000", "Focus-FocusStepSize_(nm)": "100", "PRIME-PixelType": "16bit", "COM4-DataBits": "8", "470 AO-IOChannel": "Dev1/ao2", "520 AO-MinVolts": "0.0000", "COM9-Parity": "None", "PRIME-CircularBufferEnabled": "ON", "PRIME-PP 6 Reset": "No", "640 AO-Description": "NI DAC", "Source": "PRIME", "445 AO-MinVolts": "0.0000", "PIEZO-Name": "Stage", "445 AO-Description": "NI DAC", "PRIME-CircularBufferFrameCount": "46", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "520 AO-Volts": "4.5000", "528 Shutter-TriggerSampleRate": "100000", "XPositionUm": 62321, "OpticalPath-State": "4", "PRIME-PP 3 THRESHOLD": "80", "PRIME-PVCAM Version": "3.7.1", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Gain": "1-HDR", "640 Shutter-TriggerSequenceLength": "1000", "528 AO-SupportsTriggering": "0", "445 AO-TriggerInputLine": "/Dev1/PFI0", "OpticalPath-Name": "OpticalPath", "PRIME-PP 4 ENABLED": "Yes", "PRIME-PVCAM Adapter Version": "1.3.29", "COM3-Name": "COM3", "405 AO-MaxVolts": "5.0000", "520 Shutter-Block voltage": "false", "PRIME-Timing-ClearingTimeNs": "49344000.0000", "Core-Focus": "PIEZO", "528 AO-Block voltage": "false", "TE2000-Description": "Nikon TE2000 hub", "528 AO-MinVolts": "0.0000", "COM4-Parity": "None", "Summary": { "Slices": 11, "ChContrastMin": [ 0, 0, 0, 0, 0, 0 ], "PixelAspect": 1, "SlicesFirst": true, "Interval_ms": 0, "Time": "2019-12-17 21:58:39 -0500", "Prefix": "seq_3", "InitialPositionList": [ { "Label": "Pos0", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.435], "Focus": [16480.2], "XYStage": [ 69822.40000000001, 20100.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos1", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.425], "Focus": [16480.3], "XYStage": [ 69004, 20101 ] }, "GridColumnIndex": 0 }, { "Label": "Pos2", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.44], "Focus": [16480.1], "XYStage": [ 63119.5, 20690.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos3", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.405], "Focus": [16480.2], "XYStage": [ 62321, 20690.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos4", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.415], "Focus": [16480], "XYStage": [ 56604.100000000006, 20688.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos5", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.42000000000002], "Focus": [16480.1], "XYStage": [ 56034.200000000004, 20688.800000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos6", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.455], "Focus": [16480], "XYStage": [ 50889.3, 20496.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos7", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.43], "Focus": [16480.2], "XYStage": [ 50101.600000000006, 20496.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos8", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.67000000000002], "Focus": [16479.9], "XYStage": [ 44818.600000000006, 20496.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos9", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.67000000000002], "Focus": [16480], "XYStage": [ 43897.9, 20436.800000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos10", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.675], "Focus": [16479.9], "XYStage": [ 39670.600000000006, 20664.9 ] }, "GridColumnIndex": 0 }, { "Label": "Pos11", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.69], "Focus": [16479.9], "XYStage": [ 38734, 20664.600000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos12", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.695], "Focus": [16480.1], "XYStage": [ 34037.200000000004, 20664.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos13", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.685], "Focus": [16480.1], "XYStage": [ 33152, 20664.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos14", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.69], "Focus": [16480.1], "XYStage": [ 28122.800000000003, 20664.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos15", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.675], "Focus": [16480], "XYStage": [ 27422.9, 20664.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos16", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.64000000000001], "Focus": [16480], "XYStage": [ 23005.2, 20664.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos17", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.69], "Focus": [16479.9], "XYStage": [ 21820.600000000002, 20409.600000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos18", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.41], "Focus": [16480.1], "XYStage": [ 73697.6, -13222.800000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos19", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.455], "Focus": [16480.1], "XYStage": [ 72765.8, -13223 ] }, "GridColumnIndex": 0 }, { "Label": "Pos20", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.4], "Focus": [16480.2], "XYStage": [ 67445.5, -12303.800000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos21", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.415], "Focus": [16480.2], "XYStage": [ 66613.3, -12303.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos22", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.415], "Focus": [16480.1], "XYStage": [ 61819.4, -11936.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos23", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.405], "Focus": [16480.2], "XYStage": [ 61325.700000000004, -11937.1 ] }, "GridColumnIndex": 0 }, { "Label": "Pos24", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.46], "Focus": [16480.2], "XYStage": [ 55377, -10974.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos25", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.445], "Focus": [16480.1], "XYStage": [ 54827, -11214.800000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos26", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.435], "Focus": [16480.2], "XYStage": [ 49937.100000000006, -10919.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos27", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.425], "Focus": [16480.1], "XYStage": [ 48764, -10919.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos28", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.42000000000002], "Focus": [16480.2], "XYStage": [ 43978.4, -10920.400000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos29", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.435], "Focus": [16480.2], "XYStage": [ 43106.3, -10920.400000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos30", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.405], "Focus": [16480.3], "XYStage": [ 38212.4, -10920.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos31", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.445], "Focus": [16480.2], "XYStage": [ 37254.9, -10920.400000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos32", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.44], "Focus": [16480.3], "XYStage": [ 32472.800000000003, -11294.400000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos33", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [166.95000000000002], "Focus": [16480.2], "XYStage": [ 31420.7, -11294.400000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos34", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [166.95000000000002], "Focus": [16480.2], "XYStage": [ 26881.5, -11640 ] }, "GridColumnIndex": 0 }, { "Label": "Pos35", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [166.98], "Focus": [16480.2], "XYStage": [ 25876.100000000002, -11641.1 ] }, "GridColumnIndex": 0 } ], "z-step_um": 10, "GridRow": 0, "BitDepth": 16, "Depth": 2, "Channels": 6, "Source": "Micro-Manager", "ChColors": [ -16711681, -256, -65281, -1, -1, -16776961 ], "MetadataVersion": 10, "KeepShutterOpenChannels": true, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536, 65536 ], "CustomIntervals_ms": [], "Height": 1200, "MicroManagerVersion": "1.4.23 20180219", "GridColumn": 0, "UUID": "8c8f9d20-b128-4577-9f91-89e70f22d319", "PixelType": "GRAY16", "Width": 1200, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\Yan\\20191217-YC113-S5S6", "Frames": 1, "Comment": "", "UserName": "Nikon", "PixelSize_um": 1.1, "ROI": [ 0, 0, 1200, 1200 ], "Date": "2019-12-17", "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC", "P-DAPI-high" ], "KeepShutterOpenSlices": true, "IJType": 1, "ComputerName": "ZadorConfocal2", "Positions": 36, "PositionIndex": 3, "CameraTimeout": 20000, "TimeFirst": false }, "640 AO-Name": "AnalogIO", "Vincent-D3-Command": "Close" }, "FrameKey-0-0-4": { "COM4-StopBits": "1", "528 AO-MaxVolts": "5.0000", "XLIGHT Excitation Wheel-State": "2", "470 Shutter-Name": "AnalogIO", "Core-Shutter": "520", "555 AO-Demo": "No", "XLIGHT Dichroic-State": "2", "XYStage-StepSize-Y": "0.1000", "ZPositionUm": 167.405, "XYStage-StepSize-X": "0.1000", "PRIME-FirmwareVersion": "20.28", "PRIME-ClearCycles": "2", "520-Name": "DA Shutter", "405 Shutter-Name": "AnalogIO", "PIEZO-StepSize": "0.0050", "PRIME-ClearMode": "Pre-Exposure", "470 AO-MinVolts": "0.0000", "405 AO-SupportsTriggering": "1", "CameraChannelIndex": 0, "XYStage-StartSpeed": "500.0000", "COM9-StopBits": "2", "405 AO-Description": "NI DAC", "COM4-DelayBetweenCharsMs": "0.0000", "445 AO-Block voltage": "false", "520 AO-TriggerInputLine": "/Dev1/PFI0", "555 AO-Description": "NI DAC", "520 AO-TriggerSampleRate": "100000", "COM4-Verbose": "1", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM4-Name": "COM4", "405 Shutter-Volts": "0.0000", "Vincent-D3-Port": "COM1", "PRIME-PP 4 MIN ADU AFFECTED": "200", "470 Shutter-MinVolts": "0.0000", "PRIME-PP 3 ENABLED": "Yes", "470 AO-Description": "NI DAC", "PRIME-Description": "PVCAM API device adapter", "520 Shutter-MaxVolts": "5.0000", "520 Shutter-Volts": "5.0000", "528 AO-Sequenceable": "1", "PRIME-Binning": "1x1", "555 Shutter-MaxVolts": "5.0000", "528 Shutter-TriggerSequenceLength": "1000", "405 Shutter-Block voltage": "false", "XLIGHT Spin motor-State": "1", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "Vincent-D3-Time to open (ms)": "35.0000", "Vincent-D3-Time to close (ms)": "35.0000", "445 AO-TriggerSampleRate": "100000", "640 Shutter-IOChannel": "Dev1/ao14", "555 AO-Sequenceable": "1", "520 AO-Name": "AnalogIO", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "470 AO-Block voltage": "false", "COM1-Verbose": "1", "COM9-AnswerTimeout": "10000.0000", "528 Shutter-Name": "AnalogIO", "PRIME-Trigger Timeout (secs)": "10", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "NextFrame": 0, "470 AO-TriggerSequenceLength": "1000", "PRIME-TransposeXY": "0", "445 AO-TriggerSequenceLength": "1000", "COM1-Name": "COM1", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "COM9-DataBits": "8", "COM1-AnswerTimeout": "500.0000", "Camera": "", "TE2000-Version": "V2.50-V2.50-V2.10", "528 Shutter-Volts": "0.0000", "XLIGHT Hub-Name": "XLIGHT Hub", "PIEZO-Description": "Ludl stage driver adapter", "PositionName": "Pos3", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSampleRate": "100000", "PRIME-FanSpeedSetpoint": "High", "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "405 AO-TriggerSampleRate": "100000", "470 Shutter-Block voltage": "false", "470-State": "0", "COM3-Parity": "None", "405 AO-Volts": "0.0000", "PRIME-TransposeMirrorY": "0", "PRIME-ChipName": "GS144BSI", "470 AO-Volts": "0.0000", "PRIME-TransposeMirrorX": "0", "445 Shutter-SupportsTriggering": "0", "470 Shutter-SupportsTriggering": "0", "XLIGHT Hub-Description": "XLIGHT hub", "PRIME-PreampOffLimit": "10000", "470 AO-Sequenceable": "1", "528 Shutter-MaxVolts": "5.0000", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "Core-XYStage": "XYStage", "470 AO-TriggerSampleRate": "100000", "PRIME-Timing-PreTriggerDelayNs": "24672000.0000", "528-DA Device": "528 Shutter", "Focus-Description": "Nikon TE2000 focus adapter", "FilterBlock-State": "5", "520 AO-Demo": "No", "COM3-DelayBetweenCharsMs": "0.0000", "470 Shutter-Volts": "0.0000", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "PRIME-PP 2 THRESHOLD": "125", "PRIME-PP 4 THRESHOLD": "75", "XYStage-Acceleration": "4.0000", "470 Shutter-Demo": "No", "PRIME-Name": "PMPCIECam00", "640-DA Device": "640 Shutter", "405 AO-Block voltage": "false", "ElapsedTime-ms": 134571, "PRIME-Offset": "100", "445 AO-IOChannel": "Dev1/ao1", "640 AO-MaxVolts": "5.0000", "PositionIndex": 3, "PRIME-PP 1 ENABLED": "Yes", "COM1-BaudRate": "9600", "640-State": "0", "COM3-Verbose": "1", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "LudlController-TransmissionDelay": "0", "PRIME-FullWellCapacity": "62000", "PRIME-SMARTStreamingEnabled": "No", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "640 Shutter-Description": "NI DAC", "528-Description": "DA device that is used as a shutter", "COM4-Description": "Serial port driver (boost:asio)", "405 Shutter-Description": "NI DAC", "TE2000-Name": "TE2000", "528 Shutter-Description": "NI DAC", "COM9-Description": "Serial port driver (boost:asio)", "LudlController-Name": "LudlController", "445 Shutter-TriggerSequenceLength": "1000", "PRIME-SerialNumber": "A18B203008", "445 AO-Volts": "0.0000", "PRIME-X-dimension": "1200", "PRIME-ShutterCloseDelay": "0", "XLIGHT Disk slider-State": "2", "Position": "Default", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 AO-IOChannel": "Dev1/ao5", "520 AO-IOChannel": "Dev1/ao4", "528 AO-TriggerSequenceLength": "1000", "405 Shutter-MaxVolts": "5.0000", "470-DA Device": "470 Shutter", "PRIME-FTCapable": "No", "Core-ChannelGroup": "Channels", "555 AO-SupportsTriggering": "0", "PRIME-Y-dimension": "1200", "Frame": 0, "555 Shutter-Sequenceable": "1", "PRIME-Timing-ExposureTimeNs": "9992000.0000", "Core-ImageProcessor": "", "528-State": "0", "520 AO-Block voltage": "false", "XLIGHT Excitation Wheel-Label": "CFP/YFP", "640 Shutter-Demo": "No", "COM3-StopBits": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "520 Shutter-IOChannel": "Dev1/ao11", "Width": 1200, "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-SupportsTriggering": "0", "640 AO-TriggerSampleRate": "100000", "520-Description": "DA device that is used as a shutter", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "405 Shutter-Sequenceable": "1", "405 Shutter-SupportsTriggering": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-TriggerSampleRate": "100000", "640 Shutter-MaxVolts": "5.0000", "555 AO-MaxVolts": "5.0000", "520 AO-TriggerSequenceLength": "1000", "COM3-BaudRate": "9600", "COM9-BaudRate": "9600", "Vincent-D3-Channel #": "Ch. #1", "PRIME-PMode": "Normal", "OpticalPath-Label": "Left(100)", "BitDepth": 16, "555 AO-TriggerSequenceLength": "1000", "445 Shutter-Description": "NI DAC", "405 AO-Sequenceable": "1", "PRIME-AcquisitionMethod": "Callbacks", "555 AO-Name": "AnalogIO", "PRIME-ShutterMode": "Pre-Exposure", "COM3-Handshaking": "Off", "445 AO-SupportsTriggering": "0", "ROI": "0-0-1200-1200", "COM9-Verbose": "1", "PRIME-Color": "OFF", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "PRIME-ShutterOpenDelay": "0", "COM1-Parity": "None", "Slice": 4, "SlicePosition": -10, "COM9-DelayBetweenCharsMs": "0.0000", "555-Name": "DA Shutter", "PIEZO-LudlSingleAxisName": "B", "XLIGHT Emission Wheel-Label": "565/24(G)", "640 Shutter-Name": "AnalogIO", "640 AO-Sequenceable": "1", "520 Shutter-Demo": "No", "528 Shutter-Demo": "No", "470-Name": "DA Shutter", "520-DA Device": "520 Shutter", "XYStage-Description": "Ludl XY stage driver adapter", "445 Shutter-MinVolts": "0.0000", "528 AO-TriggerSampleRate": "100000", "405 AO-MinVolts": "0.0000", "405 Shutter-TriggerSequenceLength": "1000", "Height": 1200, "555 AO-Block voltage": "false", "640 Shutter-Volts": "0.0000", "PixelType": "GRAY16", "445 Shutter-Block voltage": "false", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "520 Shutter-TriggerSequenceLength": "1000", "COM9-Name": "COM9", "Core-SLM": "", "555 AO-Volts": "0.0000", "555 Shutter-Block voltage": "false", "XYStage-Speed": "1800.0000", "520 AO-MaxVolts": "5.0000", "528 Shutter-SupportsTriggering": "0", "COM1-Description": "Serial port driver (boost:asio)", "PRIME-MetadataEnabled": "No", "520 Shutter-MinVolts": "0.0000", "470 Shutter-Sequenceable": "1", "PRIME-PreampDelay": "0", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-MinVolts": "0.0000", "ChannelIndex": 0, "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Core-AutoShutter": "1", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "Exposure-ms": 320, "PRIME-PP 2 ENABLED": "Yes", "640 Shutter-TriggerSampleRate": "100000", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "470 Shutter-IOChannel": "Dev1/ao10", "405 AO-Demo": "No", "PixelSizeUm": 1.1, "470 AO-Demo": "No", "470 Shutter-MaxVolts": "5.0000", "Vincent-D3-Name": "Vincent-D3", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FileName": "img_000000000_Il-G_004.tif", "LudlController-Reset": "Operate", "PRIME-Exposure": "320.00", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "445-Name": "DA Shutter", "555 Shutter-Name": "AnalogIO", "520 Shutter-TriggerSampleRate": "100000", "445 Shutter-IOChannel": "Dev1/ao9", "PRIME-TransposeCorrection": "0", "XYStage-StepSize": "0.1000", "445-Description": "DA device that is used as a shutter", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 AO-IOChannel": "Dev1/ao7", "520 Shutter-Name": "AnalogIO", "COM3-DataBits": "8", "640 AO-Volts": "0.0000", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "555 Shutter-IOChannel": "Dev1/ao13", "WaitInterval": null, "520 Shutter-Sequenceable": "1", "YPositionUm": 20690.300000000003, "445-State": "0", "445-DA Device": "445 Shutter", "PRIME-CircularBufferAutoSize": "ON", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "555 AO-TriggerSampleRate": "100000", "445 Shutter-Volts": "0.0000", "XLIGHT Hub-Port": "COM3", "Core-AutoFocus": "", "COM4-Handshaking": "Off", "528 Shutter-MinVolts": "0.0000", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "445 Shutter-Name": "AnalogIO", "528 Shutter-Sequenceable": "1", "XYStage-TransposeMirrorX": "0", "XYStage-TransposeMirrorY": "0", "PRIME-ReadoutRate": "100MHz 16bit", "XYStage-Name": "Stage", "640 AO-SupportsTriggering": "0", "640-Name": "DA Shutter", "640 Shutter-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "640 AO-Block voltage": "false", "445 Shutter-Sequenceable": "1", "470 AO-MaxVolts": "5.0000", "COM4-BaudRate": "9600", "470-Description": "DA device that is used as a shutter", "520 Shutter-Description": "NI DAC", "PRIME-CCDTemperature": "-14.9600", "Time": "2019-12-17 21:58:42 -0500", "640 AO-TriggerSequenceLength": "1000", "470 AO-Name": "AnalogIO", "XLIGHT Disk slider-Label": "Disk Pos 40um", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "Binning": "1x1", "TE2000-ModelType": "1", "COM3-AnswerTimeout": "500.0000", "555 Shutter-SupportsTriggering": "0", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "TE2000-Port": "COM4", "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Description": "NI DAC", "COM4-AnswerTimeout": "500.0000", "COM1-DelayBetweenCharsMs": "0.0000", "FilterBlock-Label": "Confocal", "445 AO-Name": "AnalogIO", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-Timing-ReadoutTimeNs": "24672000.0000", "528 AO-Demo": "No", "520 AO-Sequenceable": "1", "555-Description": "DA device that is used as a shutter", "405-DA Device": "405 Shutter", "405 AO-TriggerSequenceLength": "1000", "640 AO-MinVolts": "0.0000", "640 Shutter-Block voltage": "false", "FrameIndex": 0, "PRIME-Port": "CMOS", "555 AO-MinVolts": "0.0000", "COM3-Description": "Serial port driver (boost:asio)", "528 AO-Description": "NI DAC", "555 AO-IOChannel": "Dev1/ao6", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "555 AO-TriggerInputLine": "/Dev1/PFI0", "PRIME-ActualInterval-ms": "374.3700", "LudlController-Port": "COM9", "445 Shutter-Demo": "No", "PRIME-Trigger-Expose Out-Mux": "1", "SliceIndex": 4, "PRIME-CCDTemperatureSetPoint": "-15.0000", "405 Shutter-MinVolts": "0.0000", "PRIME-ExposeOutMode": "First Row", "AxisPositions": null, "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Volts": "0.0000", "528-Name": "DA Shutter", "555-State": "0", "XLIGHT Dichroic-Label": "CFP/YFP", "445 AO-Sequenceable": "1", "405-Name": "DA Shutter", "405 AO-IOChannel": "Dev1/ao0", "Core-Camera": "PRIME", "Focus-Name": "Focus", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "405 Shutter-IOChannel": "Dev1/ao8", "640 AO-Demo": "No", "640 Shutter-SupportsTriggering": "0", "445 AO-Demo": "No", "555-DA Device": "555 Shutter", "470 Shutter-Description": "NI DAC", "528 AO-Volts": "0.0000", "COM1-DataBits": "8", "Vincent-D3-Address": "x", "520 AO-SupportsTriggering": "0", "405-Description": "DA device that is used as a shutter", "640 Shutter-MinVolts": "0.0000", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "405 AO-Name": "AnalogIO", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "640 AO-TriggerInputLine": "/Dev1/PFI0", "405 AO-TriggerInputLine": "/Dev1/PFI0", "640-Description": "DA device that is used as a shutter", "445 Shutter-TriggerSampleRate": "100000", "470 Shutter-TriggerSequenceLength": "1000", "COM1-StopBits": "1", "520 Shutter-SupportsTriggering": "0", "405-State": "0", "Core-Initialize": "1", "XLIGHT Emission Wheel-State": "7", "520 AO-Description": "NI DAC", "528 AO-TriggerInputLine": "/Dev1/PFI0", "445 Shutter-MaxVolts": "5.0000", "COM9-Handshaking": "Off", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "XLIGHT Spin motor-Label": "On (spinning)", "PRIME-PP 5 ENABLED": "No", "UUID": "87d58623-8ec3-449c-bd54-db211e20d88e", "PRIME-TriggerMode": "Internal Trigger", "COM1-Handshaking": "Off", "PRIME-Timing-PostTriggerDelayNs": "49344000.0000", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "Focus-Position": "16479.8000", "528 Shutter-Block voltage": "false", "Channel": "Il-G", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "555 Shutter-Demo": "No", "405 Shutter-Demo": "No", "528 AO-Name": "AnalogIO", "520-State": "1", "Core-Galvo": "", "405 Shutter-TriggerSampleRate": "100000", "Focus-FocusStepSize_(nm)": "100", "PRIME-PixelType": "16bit", "COM4-DataBits": "8", "470 AO-IOChannel": "Dev1/ao2", "520 AO-MinVolts": "0.0000", "COM9-Parity": "None", "PRIME-CircularBufferEnabled": "ON", "PRIME-PP 6 Reset": "No", "640 AO-Description": "NI DAC", "Source": "PRIME", "445 AO-MinVolts": "0.0000", "PIEZO-Name": "Stage", "445 AO-Description": "NI DAC", "PRIME-CircularBufferFrameCount": "46", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "520 AO-Volts": "4.5000", "528 Shutter-TriggerSampleRate": "100000", "XPositionUm": 62321, "OpticalPath-State": "4", "PRIME-PP 3 THRESHOLD": "80", "PRIME-PVCAM Version": "3.7.1", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Gain": "1-HDR", "640 Shutter-TriggerSequenceLength": "1000", "528 AO-SupportsTriggering": "0", "445 AO-TriggerInputLine": "/Dev1/PFI0", "OpticalPath-Name": "OpticalPath", "PRIME-PP 4 ENABLED": "Yes", "PRIME-PVCAM Adapter Version": "1.3.29", "COM3-Name": "COM3", "405 AO-MaxVolts": "5.0000", "520 Shutter-Block voltage": "false", "PRIME-Timing-ClearingTimeNs": "49344000.0000", "Core-Focus": "PIEZO", "528 AO-Block voltage": "false", "TE2000-Description": "Nikon TE2000 hub", "528 AO-MinVolts": "0.0000", "COM4-Parity": "None", "Summary": { "Slices": 11, "ChContrastMin": [ 0, 0, 0, 0, 0, 0 ], "PixelAspect": 1, "SlicesFirst": true, "Interval_ms": 0, "Time": "2019-12-17 21:58:39 -0500", "Prefix": "seq_3", "InitialPositionList": [ { "Label": "Pos0", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.435], "Focus": [16480.2], "XYStage": [ 69822.40000000001, 20100.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos1", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.425], "Focus": [16480.3], "XYStage": [ 69004, 20101 ] }, "GridColumnIndex": 0 }, { "Label": "Pos2", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.44], "Focus": [16480.1], "XYStage": [ 63119.5, 20690.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos3", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.405], "Focus": [16480.2], "XYStage": [ 62321, 20690.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos4", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.415], "Focus": [16480], "XYStage": [ 56604.100000000006, 20688.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos5", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.42000000000002], "Focus": [16480.1], "XYStage": [ 56034.200000000004, 20688.800000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos6", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.455], "Focus": [16480], "XYStage": [ 50889.3, 20496.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos7", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.43], "Focus": [16480.2], "XYStage": [ 50101.600000000006, 20496.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos8", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.67000000000002], "Focus": [16479.9], "XYStage": [ 44818.600000000006, 20496.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos9", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.67000000000002], "Focus": [16480], "XYStage": [ 43897.9, 20436.800000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos10", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.675], "Focus": [16479.9], "XYStage": [ 39670.600000000006, 20664.9 ] }, "GridColumnIndex": 0 }, { "Label": "Pos11", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.69], "Focus": [16479.9], "XYStage": [ 38734, 20664.600000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos12", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.695], "Focus": [16480.1], "XYStage": [ 34037.200000000004, 20664.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos13", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.685], "Focus": [16480.1], "XYStage": [ 33152, 20664.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos14", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.69], "Focus": [16480.1], "XYStage": [ 28122.800000000003, 20664.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos15", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.675], "Focus": [16480], "XYStage": [ 27422.9, 20664.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos16", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.64000000000001], "Focus": [16480], "XYStage": [ 23005.2, 20664.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos17", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.69], "Focus": [16479.9], "XYStage": [ 21820.600000000002, 20409.600000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos18", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.41], "Focus": [16480.1], "XYStage": [ 73697.6, -13222.800000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos19", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.455], "Focus": [16480.1], "XYStage": [ 72765.8, -13223 ] }, "GridColumnIndex": 0 }, { "Label": "Pos20", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.4], "Focus": [16480.2], "XYStage": [ 67445.5, -12303.800000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos21", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.415], "Focus": [16480.2], "XYStage": [ 66613.3, -12303.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos22", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.415], "Focus": [16480.1], "XYStage": [ 61819.4, -11936.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos23", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.405], "Focus": [16480.2], "XYStage": [ 61325.700000000004, -11937.1 ] }, "GridColumnIndex": 0 }, { "Label": "Pos24", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.46], "Focus": [16480.2], "XYStage": [ 55377, -10974.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos25", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.445], "Focus": [16480.1], "XYStage": [ 54827, -11214.800000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos26", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.435], "Focus": [16480.2], "XYStage": [ 49937.100000000006, -10919.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos27", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.425], "Focus": [16480.1], "XYStage": [ 48764, -10919.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos28", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.42000000000002], "Focus": [16480.2], "XYStage": [ 43978.4, -10920.400000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos29", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.435], "Focus": [16480.2], "XYStage": [ 43106.3, -10920.400000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos30", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.405], "Focus": [16480.3], "XYStage": [ 38212.4, -10920.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos31", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.445], "Focus": [16480.2], "XYStage": [ 37254.9, -10920.400000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos32", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.44], "Focus": [16480.3], "XYStage": [ 32472.800000000003, -11294.400000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos33", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [166.95000000000002], "Focus": [16480.2], "XYStage": [ 31420.7, -11294.400000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos34", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [166.95000000000002], "Focus": [16480.2], "XYStage": [ 26881.5, -11640 ] }, "GridColumnIndex": 0 }, { "Label": "Pos35", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [166.98], "Focus": [16480.2], "XYStage": [ 25876.100000000002, -11641.1 ] }, "GridColumnIndex": 0 } ], "z-step_um": 10, "GridRow": 0, "BitDepth": 16, "Depth": 2, "Channels": 6, "Source": "Micro-Manager", "ChColors": [ -16711681, -256, -65281, -1, -1, -16776961 ], "MetadataVersion": 10, "KeepShutterOpenChannels": true, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536, 65536 ], "CustomIntervals_ms": [], "Height": 1200, "MicroManagerVersion": "1.4.23 20180219", "GridColumn": 0, "UUID": "8c8f9d20-b128-4577-9f91-89e70f22d319", "PixelType": "GRAY16", "Width": 1200, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\Yan\\20191217-YC113-S5S6", "Frames": 1, "Comment": "", "UserName": "Nikon", "PixelSize_um": 1.1, "ROI": [ 0, 0, 1200, 1200 ], "Date": "2019-12-17", "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC", "P-DAPI-high" ], "KeepShutterOpenSlices": true, "IJType": 1, "ComputerName": "ZadorConfocal2", "Positions": 36, "PositionIndex": 3, "CameraTimeout": 20000, "TimeFirst": false }, "640 AO-Name": "AnalogIO", "Vincent-D3-Command": "Close" }, "FrameKey-0-0-5": { "COM4-StopBits": "1", "528 AO-MaxVolts": "5.0000", "XLIGHT Excitation Wheel-State": "2", "470 Shutter-Name": "AnalogIO", "Core-Shutter": "520", "555 AO-Demo": "No", "XLIGHT Dichroic-State": "2", "XYStage-StepSize-Y": "0.1000", "ZPositionUm": 177.405, "XYStage-StepSize-X": "0.1000", "PRIME-FirmwareVersion": "20.28", "PRIME-ClearCycles": "2", "520-Name": "DA Shutter", "405 Shutter-Name": "AnalogIO", "PIEZO-StepSize": "0.0050", "PRIME-ClearMode": "Pre-Exposure", "470 AO-MinVolts": "0.0000", "405 AO-SupportsTriggering": "1", "CameraChannelIndex": 0, "XYStage-StartSpeed": "500.0000", "COM9-StopBits": "2", "405 AO-Description": "NI DAC", "COM4-DelayBetweenCharsMs": "0.0000", "445 AO-Block voltage": "false", "520 AO-TriggerInputLine": "/Dev1/PFI0", "555 AO-Description": "NI DAC", "520 AO-TriggerSampleRate": "100000", "COM4-Verbose": "1", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM4-Name": "COM4", "405 Shutter-Volts": "0.0000", "Vincent-D3-Port": "COM1", "PRIME-PP 4 MIN ADU AFFECTED": "200", "470 Shutter-MinVolts": "0.0000", "PRIME-PP 3 ENABLED": "Yes", "470 AO-Description": "NI DAC", "PRIME-Description": "PVCAM API device adapter", "520 Shutter-MaxVolts": "5.0000", "520 Shutter-Volts": "5.0000", "528 AO-Sequenceable": "1", "PRIME-Binning": "1x1", "555 Shutter-MaxVolts": "5.0000", "528 Shutter-TriggerSequenceLength": "1000", "405 Shutter-Block voltage": "false", "XLIGHT Spin motor-State": "1", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "Vincent-D3-Time to open (ms)": "35.0000", "Vincent-D3-Time to close (ms)": "35.0000", "445 AO-TriggerSampleRate": "100000", "640 Shutter-IOChannel": "Dev1/ao14", "555 AO-Sequenceable": "1", "520 AO-Name": "AnalogIO", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "470 AO-Block voltage": "false", "COM1-Verbose": "1", "COM9-AnswerTimeout": "10000.0000", "528 Shutter-Name": "AnalogIO", "PRIME-Trigger Timeout (secs)": "10", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "NextFrame": 0, "470 AO-TriggerSequenceLength": "1000", "PRIME-TransposeXY": "0", "445 AO-TriggerSequenceLength": "1000", "COM1-Name": "COM1", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "COM9-DataBits": "8", "COM1-AnswerTimeout": "500.0000", "Camera": "", "TE2000-Version": "V2.50-V2.50-V2.10", "528 Shutter-Volts": "0.0000", "XLIGHT Hub-Name": "XLIGHT Hub", "PIEZO-Description": "Ludl stage driver adapter", "PositionName": "Pos3", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSampleRate": "100000", "PRIME-FanSpeedSetpoint": "High", "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "405 AO-TriggerSampleRate": "100000", "470 Shutter-Block voltage": "false", "470-State": "0", "COM3-Parity": "None", "405 AO-Volts": "0.0000", "PRIME-TransposeMirrorY": "0", "PRIME-ChipName": "GS144BSI", "470 AO-Volts": "0.0000", "PRIME-TransposeMirrorX": "0", "445 Shutter-SupportsTriggering": "0", "470 Shutter-SupportsTriggering": "0", "XLIGHT Hub-Description": "XLIGHT hub", "PRIME-PreampOffLimit": "10000", "470 AO-Sequenceable": "1", "528 Shutter-MaxVolts": "5.0000", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "Core-XYStage": "XYStage", "470 AO-TriggerSampleRate": "100000", "PRIME-Timing-PreTriggerDelayNs": "24672000.0000", "528-DA Device": "528 Shutter", "Focus-Description": "Nikon TE2000 focus adapter", "FilterBlock-State": "5", "520 AO-Demo": "No", "COM3-DelayBetweenCharsMs": "0.0000", "470 Shutter-Volts": "0.0000", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "PRIME-PP 2 THRESHOLD": "125", "PRIME-PP 4 THRESHOLD": "75", "XYStage-Acceleration": "4.0000", "470 Shutter-Demo": "No", "PRIME-Name": "PMPCIECam00", "640-DA Device": "640 Shutter", "405 AO-Block voltage": "false", "ElapsedTime-ms": 135130, "PRIME-Offset": "100", "445 AO-IOChannel": "Dev1/ao1", "640 AO-MaxVolts": "5.0000", "PositionIndex": 3, "PRIME-PP 1 ENABLED": "Yes", "COM1-BaudRate": "9600", "640-State": "0", "COM3-Verbose": "1", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "LudlController-TransmissionDelay": "0", "PRIME-FullWellCapacity": "62000", "PRIME-SMARTStreamingEnabled": "No", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "640 Shutter-Description": "NI DAC", "528-Description": "DA device that is used as a shutter", "COM4-Description": "Serial port driver (boost:asio)", "405 Shutter-Description": "NI DAC", "TE2000-Name": "TE2000", "528 Shutter-Description": "NI DAC", "COM9-Description": "Serial port driver (boost:asio)", "LudlController-Name": "LudlController", "445 Shutter-TriggerSequenceLength": "1000", "PRIME-SerialNumber": "A18B203008", "445 AO-Volts": "0.0000", "PRIME-X-dimension": "1200", "PRIME-ShutterCloseDelay": "0", "XLIGHT Disk slider-State": "2", "Position": "Default", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 AO-IOChannel": "Dev1/ao5", "520 AO-IOChannel": "Dev1/ao4", "528 AO-TriggerSequenceLength": "1000", "405 Shutter-MaxVolts": "5.0000", "470-DA Device": "470 Shutter", "PRIME-FTCapable": "No", "Core-ChannelGroup": "Channels", "555 AO-SupportsTriggering": "0", "PRIME-Y-dimension": "1200", "Frame": 0, "555 Shutter-Sequenceable": "1", "PRIME-Timing-ExposureTimeNs": "9992000.0000", "Core-ImageProcessor": "", "528-State": "0", "520 AO-Block voltage": "false", "XLIGHT Excitation Wheel-Label": "CFP/YFP", "640 Shutter-Demo": "No", "COM3-StopBits": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "520 Shutter-IOChannel": "Dev1/ao11", "Width": 1200, "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-SupportsTriggering": "0", "640 AO-TriggerSampleRate": "100000", "520-Description": "DA device that is used as a shutter", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "405 Shutter-Sequenceable": "1", "405 Shutter-SupportsTriggering": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-TriggerSampleRate": "100000", "640 Shutter-MaxVolts": "5.0000", "555 AO-MaxVolts": "5.0000", "520 AO-TriggerSequenceLength": "1000", "COM3-BaudRate": "9600", "COM9-BaudRate": "9600", "Vincent-D3-Channel #": "Ch. #1", "PRIME-PMode": "Normal", "OpticalPath-Label": "Left(100)", "BitDepth": 16, "555 AO-TriggerSequenceLength": "1000", "445 Shutter-Description": "NI DAC", "405 AO-Sequenceable": "1", "PRIME-AcquisitionMethod": "Callbacks", "555 AO-Name": "AnalogIO", "PRIME-ShutterMode": "Pre-Exposure", "COM3-Handshaking": "Off", "445 AO-SupportsTriggering": "0", "ROI": "0-0-1200-1200", "COM9-Verbose": "1", "PRIME-Color": "OFF", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "PRIME-ShutterOpenDelay": "0", "COM1-Parity": "None", "Slice": 5, "SlicePosition": 0, "COM9-DelayBetweenCharsMs": "0.0000", "555-Name": "DA Shutter", "PIEZO-LudlSingleAxisName": "B", "XLIGHT Emission Wheel-Label": "565/24(G)", "640 Shutter-Name": "AnalogIO", "640 AO-Sequenceable": "1", "520 Shutter-Demo": "No", "528 Shutter-Demo": "No", "470-Name": "DA Shutter", "520-DA Device": "520 Shutter", "XYStage-Description": "Ludl XY stage driver adapter", "445 Shutter-MinVolts": "0.0000", "528 AO-TriggerSampleRate": "100000", "405 AO-MinVolts": "0.0000", "405 Shutter-TriggerSequenceLength": "1000", "Height": 1200, "555 AO-Block voltage": "false", "640 Shutter-Volts": "0.0000", "PixelType": "GRAY16", "445 Shutter-Block voltage": "false", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "520 Shutter-TriggerSequenceLength": "1000", "COM9-Name": "COM9", "Core-SLM": "", "555 AO-Volts": "0.0000", "555 Shutter-Block voltage": "false", "XYStage-Speed": "1800.0000", "520 AO-MaxVolts": "5.0000", "528 Shutter-SupportsTriggering": "0", "COM1-Description": "Serial port driver (boost:asio)", "PRIME-MetadataEnabled": "No", "520 Shutter-MinVolts": "0.0000", "470 Shutter-Sequenceable": "1", "PRIME-PreampDelay": "0", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-MinVolts": "0.0000", "ChannelIndex": 0, "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Core-AutoShutter": "1", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "Exposure-ms": 320, "PRIME-PP 2 ENABLED": "Yes", "640 Shutter-TriggerSampleRate": "100000", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "470 Shutter-IOChannel": "Dev1/ao10", "405 AO-Demo": "No", "PixelSizeUm": 1.1, "470 AO-Demo": "No", "470 Shutter-MaxVolts": "5.0000", "Vincent-D3-Name": "Vincent-D3", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FileName": "img_000000000_Il-G_005.tif", "LudlController-Reset": "Operate", "PRIME-Exposure": "320.00", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "445-Name": "DA Shutter", "555 Shutter-Name": "AnalogIO", "520 Shutter-TriggerSampleRate": "100000", "445 Shutter-IOChannel": "Dev1/ao9", "PRIME-TransposeCorrection": "0", "XYStage-StepSize": "0.1000", "445-Description": "DA device that is used as a shutter", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 AO-IOChannel": "Dev1/ao7", "520 Shutter-Name": "AnalogIO", "COM3-DataBits": "8", "640 AO-Volts": "0.0000", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "555 Shutter-IOChannel": "Dev1/ao13", "WaitInterval": null, "520 Shutter-Sequenceable": "1", "YPositionUm": 20690.300000000003, "445-State": "0", "445-DA Device": "445 Shutter", "PRIME-CircularBufferAutoSize": "ON", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "555 AO-TriggerSampleRate": "100000", "445 Shutter-Volts": "0.0000", "XLIGHT Hub-Port": "COM3", "Core-AutoFocus": "", "COM4-Handshaking": "Off", "528 Shutter-MinVolts": "0.0000", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "445 Shutter-Name": "AnalogIO", "528 Shutter-Sequenceable": "1", "XYStage-TransposeMirrorX": "0", "XYStage-TransposeMirrorY": "0", "PRIME-ReadoutRate": "100MHz 16bit", "XYStage-Name": "Stage", "640 AO-SupportsTriggering": "0", "640-Name": "DA Shutter", "640 Shutter-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "640 AO-Block voltage": "false", "445 Shutter-Sequenceable": "1", "470 AO-MaxVolts": "5.0000", "COM4-BaudRate": "9600", "470-Description": "DA device that is used as a shutter", "520 Shutter-Description": "NI DAC", "PRIME-CCDTemperature": "-14.9600", "Time": "2019-12-17 21:58:42 -0500", "640 AO-TriggerSequenceLength": "1000", "470 AO-Name": "AnalogIO", "XLIGHT Disk slider-Label": "Disk Pos 40um", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "Binning": "1x1", "TE2000-ModelType": "1", "COM3-AnswerTimeout": "500.0000", "555 Shutter-SupportsTriggering": "0", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "TE2000-Port": "COM4", "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Description": "NI DAC", "COM4-AnswerTimeout": "500.0000", "COM1-DelayBetweenCharsMs": "0.0000", "FilterBlock-Label": "Confocal", "445 AO-Name": "AnalogIO", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-Timing-ReadoutTimeNs": "24672000.0000", "528 AO-Demo": "No", "520 AO-Sequenceable": "1", "555-Description": "DA device that is used as a shutter", "405-DA Device": "405 Shutter", "405 AO-TriggerSequenceLength": "1000", "640 AO-MinVolts": "0.0000", "640 Shutter-Block voltage": "false", "FrameIndex": 0, "PRIME-Port": "CMOS", "555 AO-MinVolts": "0.0000", "COM3-Description": "Serial port driver (boost:asio)", "528 AO-Description": "NI DAC", "555 AO-IOChannel": "Dev1/ao6", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "555 AO-TriggerInputLine": "/Dev1/PFI0", "PRIME-ActualInterval-ms": "374.3700", "LudlController-Port": "COM9", "445 Shutter-Demo": "No", "PRIME-Trigger-Expose Out-Mux": "1", "SliceIndex": 5, "PRIME-CCDTemperatureSetPoint": "-15.0000", "405 Shutter-MinVolts": "0.0000", "PRIME-ExposeOutMode": "First Row", "AxisPositions": null, "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Volts": "0.0000", "528-Name": "DA Shutter", "555-State": "0", "XLIGHT Dichroic-Label": "CFP/YFP", "445 AO-Sequenceable": "1", "405-Name": "DA Shutter", "405 AO-IOChannel": "Dev1/ao0", "Core-Camera": "PRIME", "Focus-Name": "Focus", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "405 Shutter-IOChannel": "Dev1/ao8", "640 AO-Demo": "No", "640 Shutter-SupportsTriggering": "0", "445 AO-Demo": "No", "555-DA Device": "555 Shutter", "470 Shutter-Description": "NI DAC", "528 AO-Volts": "0.0000", "COM1-DataBits": "8", "Vincent-D3-Address": "x", "520 AO-SupportsTriggering": "0", "405-Description": "DA device that is used as a shutter", "640 Shutter-MinVolts": "0.0000", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "405 AO-Name": "AnalogIO", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "640 AO-TriggerInputLine": "/Dev1/PFI0", "405 AO-TriggerInputLine": "/Dev1/PFI0", "640-Description": "DA device that is used as a shutter", "445 Shutter-TriggerSampleRate": "100000", "470 Shutter-TriggerSequenceLength": "1000", "COM1-StopBits": "1", "520 Shutter-SupportsTriggering": "0", "405-State": "0", "Core-Initialize": "1", "XLIGHT Emission Wheel-State": "7", "520 AO-Description": "NI DAC", "528 AO-TriggerInputLine": "/Dev1/PFI0", "445 Shutter-MaxVolts": "5.0000", "COM9-Handshaking": "Off", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "XLIGHT Spin motor-Label": "On (spinning)", "PRIME-PP 5 ENABLED": "No", "UUID": "9e7c2359-ae6a-4bbb-8d59-7b3344c446ac", "PRIME-TriggerMode": "Internal Trigger", "COM1-Handshaking": "Off", "PRIME-Timing-PostTriggerDelayNs": "49344000.0000", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "Focus-Position": "16479.8000", "528 Shutter-Block voltage": "false", "Channel": "Il-G", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "555 Shutter-Demo": "No", "405 Shutter-Demo": "No", "528 AO-Name": "AnalogIO", "520-State": "1", "Core-Galvo": "", "405 Shutter-TriggerSampleRate": "100000", "Focus-FocusStepSize_(nm)": "100", "PRIME-PixelType": "16bit", "COM4-DataBits": "8", "470 AO-IOChannel": "Dev1/ao2", "520 AO-MinVolts": "0.0000", "COM9-Parity": "None", "PRIME-CircularBufferEnabled": "ON", "PRIME-PP 6 Reset": "No", "640 AO-Description": "NI DAC", "Source": "PRIME", "445 AO-MinVolts": "0.0000", "PIEZO-Name": "Stage", "445 AO-Description": "NI DAC", "PRIME-CircularBufferFrameCount": "46", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "520 AO-Volts": "4.5000", "528 Shutter-TriggerSampleRate": "100000", "XPositionUm": 62321, "OpticalPath-State": "4", "PRIME-PP 3 THRESHOLD": "80", "PRIME-PVCAM Version": "3.7.1", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Gain": "1-HDR", "640 Shutter-TriggerSequenceLength": "1000", "528 AO-SupportsTriggering": "0", "445 AO-TriggerInputLine": "/Dev1/PFI0", "OpticalPath-Name": "OpticalPath", "PRIME-PP 4 ENABLED": "Yes", "PRIME-PVCAM Adapter Version": "1.3.29", "COM3-Name": "COM3", "405 AO-MaxVolts": "5.0000", "520 Shutter-Block voltage": "false", "PRIME-Timing-ClearingTimeNs": "49344000.0000", "Core-Focus": "PIEZO", "528 AO-Block voltage": "false", "TE2000-Description": "Nikon TE2000 hub", "528 AO-MinVolts": "0.0000", "COM4-Parity": "None", "Summary": { "Slices": 11, "ChContrastMin": [ 0, 0, 0, 0, 0, 0 ], "PixelAspect": 1, "SlicesFirst": true, "Interval_ms": 0, "Time": "2019-12-17 21:58:39 -0500", "Prefix": "seq_3", "InitialPositionList": [ { "Label": "Pos0", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.435], "Focus": [16480.2], "XYStage": [ 69822.40000000001, 20100.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos1", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.425], "Focus": [16480.3], "XYStage": [ 69004, 20101 ] }, "GridColumnIndex": 0 }, { "Label": "Pos2", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.44], "Focus": [16480.1], "XYStage": [ 63119.5, 20690.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos3", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.405], "Focus": [16480.2], "XYStage": [ 62321, 20690.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos4", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.415], "Focus": [16480], "XYStage": [ 56604.100000000006, 20688.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos5", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.42000000000002], "Focus": [16480.1], "XYStage": [ 56034.200000000004, 20688.800000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos6", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.455], "Focus": [16480], "XYStage": [ 50889.3, 20496.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos7", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.43], "Focus": [16480.2], "XYStage": [ 50101.600000000006, 20496.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos8", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.67000000000002], "Focus": [16479.9], "XYStage": [ 44818.600000000006, 20496.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos9", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.67000000000002], "Focus": [16480], "XYStage": [ 43897.9, 20436.800000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos10", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.675], "Focus": [16479.9], "XYStage": [ 39670.600000000006, 20664.9 ] }, "GridColumnIndex": 0 }, { "Label": "Pos11", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.69], "Focus": [16479.9], "XYStage": [ 38734, 20664.600000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos12", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.695], "Focus": [16480.1], "XYStage": [ 34037.200000000004, 20664.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos13", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.685], "Focus": [16480.1], "XYStage": [ 33152, 20664.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos14", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.69], "Focus": [16480.1], "XYStage": [ 28122.800000000003, 20664.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos15", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.675], "Focus": [16480], "XYStage": [ 27422.9, 20664.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos16", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.64000000000001], "Focus": [16480], "XYStage": [ 23005.2, 20664.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos17", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.69], "Focus": [16479.9], "XYStage": [ 21820.600000000002, 20409.600000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos18", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.41], "Focus": [16480.1], "XYStage": [ 73697.6, -13222.800000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos19", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.455], "Focus": [16480.1], "XYStage": [ 72765.8, -13223 ] }, "GridColumnIndex": 0 }, { "Label": "Pos20", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.4], "Focus": [16480.2], "XYStage": [ 67445.5, -12303.800000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos21", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.415], "Focus": [16480.2], "XYStage": [ 66613.3, -12303.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos22", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.415], "Focus": [16480.1], "XYStage": [ 61819.4, -11936.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos23", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.405], "Focus": [16480.2], "XYStage": [ 61325.700000000004, -11937.1 ] }, "GridColumnIndex": 0 }, { "Label": "Pos24", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.46], "Focus": [16480.2], "XYStage": [ 55377, -10974.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos25", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.445], "Focus": [16480.1], "XYStage": [ 54827, -11214.800000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos26", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.435], "Focus": [16480.2], "XYStage": [ 49937.100000000006, -10919.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos27", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.425], "Focus": [16480.1], "XYStage": [ 48764, -10919.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos28", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.42000000000002], "Focus": [16480.2], "XYStage": [ 43978.4, -10920.400000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos29", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.435], "Focus": [16480.2], "XYStage": [ 43106.3, -10920.400000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos30", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.405], "Focus": [16480.3], "XYStage": [ 38212.4, -10920.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos31", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.445], "Focus": [16480.2], "XYStage": [ 37254.9, -10920.400000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos32", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.44], "Focus": [16480.3], "XYStage": [ 32472.800000000003, -11294.400000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos33", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [166.95000000000002], "Focus": [16480.2], "XYStage": [ 31420.7, -11294.400000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos34", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [166.95000000000002], "Focus": [16480.2], "XYStage": [ 26881.5, -11640 ] }, "GridColumnIndex": 0 }, { "Label": "Pos35", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [166.98], "Focus": [16480.2], "XYStage": [ 25876.100000000002, -11641.1 ] }, "GridColumnIndex": 0 } ], "z-step_um": 10, "GridRow": 0, "BitDepth": 16, "Depth": 2, "Channels": 6, "Source": "Micro-Manager", "ChColors": [ -16711681, -256, -65281, -1, -1, -16776961 ], "MetadataVersion": 10, "KeepShutterOpenChannels": true, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536, 65536 ], "CustomIntervals_ms": [], "Height": 1200, "MicroManagerVersion": "1.4.23 20180219", "GridColumn": 0, "UUID": "8c8f9d20-b128-4577-9f91-89e70f22d319", "PixelType": "GRAY16", "Width": 1200, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\Yan\\20191217-YC113-S5S6", "Frames": 1, "Comment": "", "UserName": "Nikon", "PixelSize_um": 1.1, "ROI": [ 0, 0, 1200, 1200 ], "Date": "2019-12-17", "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC", "P-DAPI-high" ], "KeepShutterOpenSlices": true, "IJType": 1, "ComputerName": "ZadorConfocal2", "Positions": 36, "PositionIndex": 3, "CameraTimeout": 20000, "TimeFirst": false }, "640 AO-Name": "AnalogIO", "Vincent-D3-Command": "Close" }, "FrameKey-0-0-6": { "COM4-StopBits": "1", "528 AO-MaxVolts": "5.0000", "XLIGHT Excitation Wheel-State": "2", "470 Shutter-Name": "AnalogIO", "Core-Shutter": "520", "555 AO-Demo": "No", "XLIGHT Dichroic-State": "2", "XYStage-StepSize-Y": "0.1000", "ZPositionUm": 187.405, "XYStage-StepSize-X": "0.1000", "PRIME-FirmwareVersion": "20.28", "PRIME-ClearCycles": "2", "520-Name": "DA Shutter", "405 Shutter-Name": "AnalogIO", "PIEZO-StepSize": "0.0050", "PRIME-ClearMode": "Pre-Exposure", "470 AO-MinVolts": "0.0000", "405 AO-SupportsTriggering": "1", "CameraChannelIndex": 0, "XYStage-StartSpeed": "500.0000", "COM9-StopBits": "2", "405 AO-Description": "NI DAC", "COM4-DelayBetweenCharsMs": "0.0000", "445 AO-Block voltage": "false", "520 AO-TriggerInputLine": "/Dev1/PFI0", "555 AO-Description": "NI DAC", "520 AO-TriggerSampleRate": "100000", "COM4-Verbose": "1", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM4-Name": "COM4", "405 Shutter-Volts": "0.0000", "Vincent-D3-Port": "COM1", "PRIME-PP 4 MIN ADU AFFECTED": "200", "470 Shutter-MinVolts": "0.0000", "PRIME-PP 3 ENABLED": "Yes", "470 AO-Description": "NI DAC", "PRIME-Description": "PVCAM API device adapter", "520 Shutter-MaxVolts": "5.0000", "520 Shutter-Volts": "5.0000", "528 AO-Sequenceable": "1", "PRIME-Binning": "1x1", "555 Shutter-MaxVolts": "5.0000", "528 Shutter-TriggerSequenceLength": "1000", "405 Shutter-Block voltage": "false", "XLIGHT Spin motor-State": "1", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "Vincent-D3-Time to open (ms)": "35.0000", "Vincent-D3-Time to close (ms)": "35.0000", "445 AO-TriggerSampleRate": "100000", "640 Shutter-IOChannel": "Dev1/ao14", "555 AO-Sequenceable": "1", "520 AO-Name": "AnalogIO", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "470 AO-Block voltage": "false", "COM1-Verbose": "1", "COM9-AnswerTimeout": "10000.0000", "528 Shutter-Name": "AnalogIO", "PRIME-Trigger Timeout (secs)": "10", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "NextFrame": 0, "470 AO-TriggerSequenceLength": "1000", "PRIME-TransposeXY": "0", "445 AO-TriggerSequenceLength": "1000", "COM1-Name": "COM1", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "COM9-DataBits": "8", "COM1-AnswerTimeout": "500.0000", "Camera": "", "TE2000-Version": "V2.50-V2.50-V2.10", "528 Shutter-Volts": "0.0000", "XLIGHT Hub-Name": "XLIGHT Hub", "PIEZO-Description": "Ludl stage driver adapter", "PositionName": "Pos3", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSampleRate": "100000", "PRIME-FanSpeedSetpoint": "High", "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "405 AO-TriggerSampleRate": "100000", "470 Shutter-Block voltage": "false", "470-State": "0", "COM3-Parity": "None", "405 AO-Volts": "0.0000", "PRIME-TransposeMirrorY": "0", "PRIME-ChipName": "GS144BSI", "470 AO-Volts": "0.0000", "PRIME-TransposeMirrorX": "0", "445 Shutter-SupportsTriggering": "0", "470 Shutter-SupportsTriggering": "0", "XLIGHT Hub-Description": "XLIGHT hub", "PRIME-PreampOffLimit": "10000", "470 AO-Sequenceable": "1", "528 Shutter-MaxVolts": "5.0000", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "Core-XYStage": "XYStage", "470 AO-TriggerSampleRate": "100000", "PRIME-Timing-PreTriggerDelayNs": "24672000.0000", "528-DA Device": "528 Shutter", "Focus-Description": "Nikon TE2000 focus adapter", "FilterBlock-State": "5", "520 AO-Demo": "No", "COM3-DelayBetweenCharsMs": "0.0000", "470 Shutter-Volts": "0.0000", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "PRIME-PP 2 THRESHOLD": "125", "PRIME-PP 4 THRESHOLD": "75", "XYStage-Acceleration": "4.0000", "470 Shutter-Demo": "No", "PRIME-Name": "PMPCIECam00", "640-DA Device": "640 Shutter", "405 AO-Block voltage": "false", "ElapsedTime-ms": 135691, "PRIME-Offset": "100", "445 AO-IOChannel": "Dev1/ao1", "640 AO-MaxVolts": "5.0000", "PositionIndex": 3, "PRIME-PP 1 ENABLED": "Yes", "COM1-BaudRate": "9600", "640-State": "0", "COM3-Verbose": "1", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "LudlController-TransmissionDelay": "0", "PRIME-FullWellCapacity": "62000", "PRIME-SMARTStreamingEnabled": "No", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "640 Shutter-Description": "NI DAC", "528-Description": "DA device that is used as a shutter", "COM4-Description": "Serial port driver (boost:asio)", "405 Shutter-Description": "NI DAC", "TE2000-Name": "TE2000", "528 Shutter-Description": "NI DAC", "COM9-Description": "Serial port driver (boost:asio)", "LudlController-Name": "LudlController", "445 Shutter-TriggerSequenceLength": "1000", "PRIME-SerialNumber": "A18B203008", "445 AO-Volts": "0.0000", "PRIME-X-dimension": "1200", "PRIME-ShutterCloseDelay": "0", "XLIGHT Disk slider-State": "2", "Position": "Default", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 AO-IOChannel": "Dev1/ao5", "520 AO-IOChannel": "Dev1/ao4", "528 AO-TriggerSequenceLength": "1000", "405 Shutter-MaxVolts": "5.0000", "470-DA Device": "470 Shutter", "PRIME-FTCapable": "No", "Core-ChannelGroup": "Channels", "555 AO-SupportsTriggering": "0", "PRIME-Y-dimension": "1200", "Frame": 0, "555 Shutter-Sequenceable": "1", "PRIME-Timing-ExposureTimeNs": "9992000.0000", "Core-ImageProcessor": "", "528-State": "0", "520 AO-Block voltage": "false", "XLIGHT Excitation Wheel-Label": "CFP/YFP", "640 Shutter-Demo": "No", "COM3-StopBits": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "520 Shutter-IOChannel": "Dev1/ao11", "Width": 1200, "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-SupportsTriggering": "0", "640 AO-TriggerSampleRate": "100000", "520-Description": "DA device that is used as a shutter", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "405 Shutter-Sequenceable": "1", "405 Shutter-SupportsTriggering": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-TriggerSampleRate": "100000", "640 Shutter-MaxVolts": "5.0000", "555 AO-MaxVolts": "5.0000", "520 AO-TriggerSequenceLength": "1000", "COM3-BaudRate": "9600", "COM9-BaudRate": "9600", "Vincent-D3-Channel #": "Ch. #1", "PRIME-PMode": "Normal", "OpticalPath-Label": "Left(100)", "BitDepth": 16, "555 AO-TriggerSequenceLength": "1000", "445 Shutter-Description": "NI DAC", "405 AO-Sequenceable": "1", "PRIME-AcquisitionMethod": "Callbacks", "555 AO-Name": "AnalogIO", "PRIME-ShutterMode": "Pre-Exposure", "COM3-Handshaking": "Off", "445 AO-SupportsTriggering": "0", "ROI": "0-0-1200-1200", "COM9-Verbose": "1", "PRIME-Color": "OFF", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "PRIME-ShutterOpenDelay": "0", "COM1-Parity": "None", "Slice": 6, "SlicePosition": 10, "COM9-DelayBetweenCharsMs": "0.0000", "555-Name": "DA Shutter", "PIEZO-LudlSingleAxisName": "B", "XLIGHT Emission Wheel-Label": "565/24(G)", "640 Shutter-Name": "AnalogIO", "640 AO-Sequenceable": "1", "520 Shutter-Demo": "No", "528 Shutter-Demo": "No", "470-Name": "DA Shutter", "520-DA Device": "520 Shutter", "XYStage-Description": "Ludl XY stage driver adapter", "445 Shutter-MinVolts": "0.0000", "528 AO-TriggerSampleRate": "100000", "405 AO-MinVolts": "0.0000", "405 Shutter-TriggerSequenceLength": "1000", "Height": 1200, "555 AO-Block voltage": "false", "640 Shutter-Volts": "0.0000", "PixelType": "GRAY16", "445 Shutter-Block voltage": "false", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "520 Shutter-TriggerSequenceLength": "1000", "COM9-Name": "COM9", "Core-SLM": "", "555 AO-Volts": "0.0000", "555 Shutter-Block voltage": "false", "XYStage-Speed": "1800.0000", "520 AO-MaxVolts": "5.0000", "528 Shutter-SupportsTriggering": "0", "COM1-Description": "Serial port driver (boost:asio)", "PRIME-MetadataEnabled": "No", "520 Shutter-MinVolts": "0.0000", "470 Shutter-Sequenceable": "1", "PRIME-PreampDelay": "0", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-MinVolts": "0.0000", "ChannelIndex": 0, "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Core-AutoShutter": "1", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "Exposure-ms": 320, "PRIME-PP 2 ENABLED": "Yes", "640 Shutter-TriggerSampleRate": "100000", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "470 Shutter-IOChannel": "Dev1/ao10", "405 AO-Demo": "No", "PixelSizeUm": 1.1, "470 AO-Demo": "No", "470 Shutter-MaxVolts": "5.0000", "Vincent-D3-Name": "Vincent-D3", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FileName": "img_000000000_Il-G_006.tif", "LudlController-Reset": "Operate", "PRIME-Exposure": "320.00", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "445-Name": "DA Shutter", "555 Shutter-Name": "AnalogIO", "520 Shutter-TriggerSampleRate": "100000", "445 Shutter-IOChannel": "Dev1/ao9", "PRIME-TransposeCorrection": "0", "XYStage-StepSize": "0.1000", "445-Description": "DA device that is used as a shutter", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 AO-IOChannel": "Dev1/ao7", "520 Shutter-Name": "AnalogIO", "COM3-DataBits": "8", "640 AO-Volts": "0.0000", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "555 Shutter-IOChannel": "Dev1/ao13", "WaitInterval": null, "520 Shutter-Sequenceable": "1", "YPositionUm": 20690.300000000003, "445-State": "0", "445-DA Device": "445 Shutter", "PRIME-CircularBufferAutoSize": "ON", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "555 AO-TriggerSampleRate": "100000", "445 Shutter-Volts": "0.0000", "XLIGHT Hub-Port": "COM3", "Core-AutoFocus": "", "COM4-Handshaking": "Off", "528 Shutter-MinVolts": "0.0000", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "445 Shutter-Name": "AnalogIO", "528 Shutter-Sequenceable": "1", "XYStage-TransposeMirrorX": "0", "XYStage-TransposeMirrorY": "0", "PRIME-ReadoutRate": "100MHz 16bit", "XYStage-Name": "Stage", "640 AO-SupportsTriggering": "0", "640-Name": "DA Shutter", "640 Shutter-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "640 AO-Block voltage": "false", "445 Shutter-Sequenceable": "1", "470 AO-MaxVolts": "5.0000", "COM4-BaudRate": "9600", "470-Description": "DA device that is used as a shutter", "520 Shutter-Description": "NI DAC", "PRIME-CCDTemperature": "-14.9600", "Time": "2019-12-17 21:58:43 -0500", "640 AO-TriggerSequenceLength": "1000", "470 AO-Name": "AnalogIO", "XLIGHT Disk slider-Label": "Disk Pos 40um", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "Binning": "1x1", "TE2000-ModelType": "1", "COM3-AnswerTimeout": "500.0000", "555 Shutter-SupportsTriggering": "0", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "TE2000-Port": "COM4", "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Description": "NI DAC", "COM4-AnswerTimeout": "500.0000", "COM1-DelayBetweenCharsMs": "0.0000", "FilterBlock-Label": "Confocal", "445 AO-Name": "AnalogIO", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-Timing-ReadoutTimeNs": "24672000.0000", "528 AO-Demo": "No", "520 AO-Sequenceable": "1", "555-Description": "DA device that is used as a shutter", "405-DA Device": "405 Shutter", "405 AO-TriggerSequenceLength": "1000", "640 AO-MinVolts": "0.0000", "640 Shutter-Block voltage": "false", "FrameIndex": 0, "PRIME-Port": "CMOS", "555 AO-MinVolts": "0.0000", "COM3-Description": "Serial port driver (boost:asio)", "528 AO-Description": "NI DAC", "555 AO-IOChannel": "Dev1/ao6", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "555 AO-TriggerInputLine": "/Dev1/PFI0", "PRIME-ActualInterval-ms": "374.3700", "LudlController-Port": "COM9", "445 Shutter-Demo": "No", "PRIME-Trigger-Expose Out-Mux": "1", "SliceIndex": 6, "PRIME-CCDTemperatureSetPoint": "-15.0000", "405 Shutter-MinVolts": "0.0000", "PRIME-ExposeOutMode": "First Row", "AxisPositions": null, "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Volts": "0.0000", "528-Name": "DA Shutter", "555-State": "0", "XLIGHT Dichroic-Label": "CFP/YFP", "445 AO-Sequenceable": "1", "405-Name": "DA Shutter", "405 AO-IOChannel": "Dev1/ao0", "Core-Camera": "PRIME", "Focus-Name": "Focus", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "405 Shutter-IOChannel": "Dev1/ao8", "640 AO-Demo": "No", "640 Shutter-SupportsTriggering": "0", "445 AO-Demo": "No", "555-DA Device": "555 Shutter", "470 Shutter-Description": "NI DAC", "528 AO-Volts": "0.0000", "COM1-DataBits": "8", "Vincent-D3-Address": "x", "520 AO-SupportsTriggering": "0", "405-Description": "DA device that is used as a shutter", "640 Shutter-MinVolts": "0.0000", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "405 AO-Name": "AnalogIO", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "640 AO-TriggerInputLine": "/Dev1/PFI0", "405 AO-TriggerInputLine": "/Dev1/PFI0", "640-Description": "DA device that is used as a shutter", "445 Shutter-TriggerSampleRate": "100000", "470 Shutter-TriggerSequenceLength": "1000", "COM1-StopBits": "1", "520 Shutter-SupportsTriggering": "0", "405-State": "0", "Core-Initialize": "1", "XLIGHT Emission Wheel-State": "7", "520 AO-Description": "NI DAC", "528 AO-TriggerInputLine": "/Dev1/PFI0", "445 Shutter-MaxVolts": "5.0000", "COM9-Handshaking": "Off", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "XLIGHT Spin motor-Label": "On (spinning)", "PRIME-PP 5 ENABLED": "No", "UUID": "d0320f37-58cc-4935-88f4-fc733ac9a258", "PRIME-TriggerMode": "Internal Trigger", "COM1-Handshaking": "Off", "PRIME-Timing-PostTriggerDelayNs": "49344000.0000", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "Focus-Position": "16479.8000", "528 Shutter-Block voltage": "false", "Channel": "Il-G", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "555 Shutter-Demo": "No", "405 Shutter-Demo": "No", "528 AO-Name": "AnalogIO", "520-State": "1", "Core-Galvo": "", "405 Shutter-TriggerSampleRate": "100000", "Focus-FocusStepSize_(nm)": "100", "PRIME-PixelType": "16bit", "COM4-DataBits": "8", "470 AO-IOChannel": "Dev1/ao2", "520 AO-MinVolts": "0.0000", "COM9-Parity": "None", "PRIME-CircularBufferEnabled": "ON", "PRIME-PP 6 Reset": "No", "640 AO-Description": "NI DAC", "Source": "PRIME", "445 AO-MinVolts": "0.0000", "PIEZO-Name": "Stage", "445 AO-Description": "NI DAC", "PRIME-CircularBufferFrameCount": "46", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "520 AO-Volts": "4.5000", "528 Shutter-TriggerSampleRate": "100000", "XPositionUm": 62321, "OpticalPath-State": "4", "PRIME-PP 3 THRESHOLD": "80", "PRIME-PVCAM Version": "3.7.1", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Gain": "1-HDR", "640 Shutter-TriggerSequenceLength": "1000", "528 AO-SupportsTriggering": "0", "445 AO-TriggerInputLine": "/Dev1/PFI0", "OpticalPath-Name": "OpticalPath", "PRIME-PP 4 ENABLED": "Yes", "PRIME-PVCAM Adapter Version": "1.3.29", "COM3-Name": "COM3", "405 AO-MaxVolts": "5.0000", "520 Shutter-Block voltage": "false", "PRIME-Timing-ClearingTimeNs": "49344000.0000", "Core-Focus": "PIEZO", "528 AO-Block voltage": "false", "TE2000-Description": "Nikon TE2000 hub", "528 AO-MinVolts": "0.0000", "COM4-Parity": "None", "Summary": { "Slices": 11, "ChContrastMin": [ 0, 0, 0, 0, 0, 0 ], "PixelAspect": 1, "SlicesFirst": true, "Interval_ms": 0, "Time": "2019-12-17 21:58:39 -0500", "Prefix": "seq_3", "InitialPositionList": [ { "Label": "Pos0", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.435], "Focus": [16480.2], "XYStage": [ 69822.40000000001, 20100.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos1", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.425], "Focus": [16480.3], "XYStage": [ 69004, 20101 ] }, "GridColumnIndex": 0 }, { "Label": "Pos2", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.44], "Focus": [16480.1], "XYStage": [ 63119.5, 20690.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos3", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.405], "Focus": [16480.2], "XYStage": [ 62321, 20690.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos4", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.415], "Focus": [16480], "XYStage": [ 56604.100000000006, 20688.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos5", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.42000000000002], "Focus": [16480.1], "XYStage": [ 56034.200000000004, 20688.800000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos6", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.455], "Focus": [16480], "XYStage": [ 50889.3, 20496.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos7", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.43], "Focus": [16480.2], "XYStage": [ 50101.600000000006, 20496.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos8", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.67000000000002], "Focus": [16479.9], "XYStage": [ 44818.600000000006, 20496.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos9", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.67000000000002], "Focus": [16480], "XYStage": [ 43897.9, 20436.800000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos10", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.675], "Focus": [16479.9], "XYStage": [ 39670.600000000006, 20664.9 ] }, "GridColumnIndex": 0 }, { "Label": "Pos11", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.69], "Focus": [16479.9], "XYStage": [ 38734, 20664.600000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos12", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.695], "Focus": [16480.1], "XYStage": [ 34037.200000000004, 20664.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos13", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.685], "Focus": [16480.1], "XYStage": [ 33152, 20664.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos14", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.69], "Focus": [16480.1], "XYStage": [ 28122.800000000003, 20664.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos15", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.675], "Focus": [16480], "XYStage": [ 27422.9, 20664.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos16", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.64000000000001], "Focus": [16480], "XYStage": [ 23005.2, 20664.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos17", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.69], "Focus": [16479.9], "XYStage": [ 21820.600000000002, 20409.600000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos18", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.41], "Focus": [16480.1], "XYStage": [ 73697.6, -13222.800000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos19", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.455], "Focus": [16480.1], "XYStage": [ 72765.8, -13223 ] }, "GridColumnIndex": 0 }, { "Label": "Pos20", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.4], "Focus": [16480.2], "XYStage": [ 67445.5, -12303.800000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos21", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.415], "Focus": [16480.2], "XYStage": [ 66613.3, -12303.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos22", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.415], "Focus": [16480.1], "XYStage": [ 61819.4, -11936.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos23", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.405], "Focus": [16480.2], "XYStage": [ 61325.700000000004, -11937.1 ] }, "GridColumnIndex": 0 }, { "Label": "Pos24", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.46], "Focus": [16480.2], "XYStage": [ 55377, -10974.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos25", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.445], "Focus": [16480.1], "XYStage": [ 54827, -11214.800000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos26", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.435], "Focus": [16480.2], "XYStage": [ 49937.100000000006, -10919.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos27", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.425], "Focus": [16480.1], "XYStage": [ 48764, -10919.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos28", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.42000000000002], "Focus": [16480.2], "XYStage": [ 43978.4, -10920.400000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos29", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.435], "Focus": [16480.2], "XYStage": [ 43106.3, -10920.400000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos30", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.405], "Focus": [16480.3], "XYStage": [ 38212.4, -10920.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos31", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.445], "Focus": [16480.2], "XYStage": [ 37254.9, -10920.400000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos32", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.44], "Focus": [16480.3], "XYStage": [ 32472.800000000003, -11294.400000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos33", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [166.95000000000002], "Focus": [16480.2], "XYStage": [ 31420.7, -11294.400000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos34", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [166.95000000000002], "Focus": [16480.2], "XYStage": [ 26881.5, -11640 ] }, "GridColumnIndex": 0 }, { "Label": "Pos35", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [166.98], "Focus": [16480.2], "XYStage": [ 25876.100000000002, -11641.1 ] }, "GridColumnIndex": 0 } ], "z-step_um": 10, "GridRow": 0, "BitDepth": 16, "Depth": 2, "Channels": 6, "Source": "Micro-Manager", "ChColors": [ -16711681, -256, -65281, -1, -1, -16776961 ], "MetadataVersion": 10, "KeepShutterOpenChannels": true, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536, 65536 ], "CustomIntervals_ms": [], "Height": 1200, "MicroManagerVersion": "1.4.23 20180219", "GridColumn": 0, "UUID": "8c8f9d20-b128-4577-9f91-89e70f22d319", "PixelType": "GRAY16", "Width": 1200, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\Yan\\20191217-YC113-S5S6", "Frames": 1, "Comment": "", "UserName": "Nikon", "PixelSize_um": 1.1, "ROI": [ 0, 0, 1200, 1200 ], "Date": "2019-12-17", "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC", "P-DAPI-high" ], "KeepShutterOpenSlices": true, "IJType": 1, "ComputerName": "ZadorConfocal2", "Positions": 36, "PositionIndex": 3, "CameraTimeout": 20000, "TimeFirst": false }, "640 AO-Name": "AnalogIO", "Vincent-D3-Command": "Close" }, "FrameKey-0-0-7": { "COM4-StopBits": "1", "528 AO-MaxVolts": "5.0000", "XLIGHT Excitation Wheel-State": "2", "470 Shutter-Name": "AnalogIO", "Core-Shutter": "520", "555 AO-Demo": "No", "XLIGHT Dichroic-State": "2", "XYStage-StepSize-Y": "0.1000", "ZPositionUm": 197.405, "XYStage-StepSize-X": "0.1000", "PRIME-FirmwareVersion": "20.28", "PRIME-ClearCycles": "2", "520-Name": "DA Shutter", "405 Shutter-Name": "AnalogIO", "PIEZO-StepSize": "0.0050", "PRIME-ClearMode": "Pre-Exposure", "470 AO-MinVolts": "0.0000", "405 AO-SupportsTriggering": "1", "CameraChannelIndex": 0, "XYStage-StartSpeed": "500.0000", "COM9-StopBits": "2", "405 AO-Description": "NI DAC", "COM4-DelayBetweenCharsMs": "0.0000", "445 AO-Block voltage": "false", "520 AO-TriggerInputLine": "/Dev1/PFI0", "555 AO-Description": "NI DAC", "520 AO-TriggerSampleRate": "100000", "COM4-Verbose": "1", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM4-Name": "COM4", "405 Shutter-Volts": "0.0000", "Vincent-D3-Port": "COM1", "PRIME-PP 4 MIN ADU AFFECTED": "200", "470 Shutter-MinVolts": "0.0000", "PRIME-PP 3 ENABLED": "Yes", "470 AO-Description": "NI DAC", "PRIME-Description": "PVCAM API device adapter", "520 Shutter-MaxVolts": "5.0000", "520 Shutter-Volts": "5.0000", "528 AO-Sequenceable": "1", "PRIME-Binning": "1x1", "555 Shutter-MaxVolts": "5.0000", "528 Shutter-TriggerSequenceLength": "1000", "405 Shutter-Block voltage": "false", "XLIGHT Spin motor-State": "1", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "Vincent-D3-Time to open (ms)": "35.0000", "Vincent-D3-Time to close (ms)": "35.0000", "445 AO-TriggerSampleRate": "100000", "640 Shutter-IOChannel": "Dev1/ao14", "555 AO-Sequenceable": "1", "520 AO-Name": "AnalogIO", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "470 AO-Block voltage": "false", "COM1-Verbose": "1", "COM9-AnswerTimeout": "10000.0000", "528 Shutter-Name": "AnalogIO", "PRIME-Trigger Timeout (secs)": "10", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "NextFrame": 0, "470 AO-TriggerSequenceLength": "1000", "PRIME-TransposeXY": "0", "445 AO-TriggerSequenceLength": "1000", "COM1-Name": "COM1", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "COM9-DataBits": "8", "COM1-AnswerTimeout": "500.0000", "Camera": "", "TE2000-Version": "V2.50-V2.50-V2.10", "528 Shutter-Volts": "0.0000", "XLIGHT Hub-Name": "XLIGHT Hub", "PIEZO-Description": "Ludl stage driver adapter", "PositionName": "Pos3", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSampleRate": "100000", "PRIME-FanSpeedSetpoint": "High", "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "405 AO-TriggerSampleRate": "100000", "470 Shutter-Block voltage": "false", "470-State": "0", "COM3-Parity": "None", "405 AO-Volts": "0.0000", "PRIME-TransposeMirrorY": "0", "PRIME-ChipName": "GS144BSI", "470 AO-Volts": "0.0000", "PRIME-TransposeMirrorX": "0", "445 Shutter-SupportsTriggering": "0", "470 Shutter-SupportsTriggering": "0", "XLIGHT Hub-Description": "XLIGHT hub", "PRIME-PreampOffLimit": "10000", "470 AO-Sequenceable": "1", "528 Shutter-MaxVolts": "5.0000", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "Core-XYStage": "XYStage", "470 AO-TriggerSampleRate": "100000", "PRIME-Timing-PreTriggerDelayNs": "24672000.0000", "528-DA Device": "528 Shutter", "Focus-Description": "Nikon TE2000 focus adapter", "FilterBlock-State": "5", "520 AO-Demo": "No", "COM3-DelayBetweenCharsMs": "0.0000", "470 Shutter-Volts": "0.0000", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "PRIME-PP 2 THRESHOLD": "125", "PRIME-PP 4 THRESHOLD": "75", "XYStage-Acceleration": "4.0000", "470 Shutter-Demo": "No", "PRIME-Name": "PMPCIECam00", "640-DA Device": "640 Shutter", "405 AO-Block voltage": "false", "ElapsedTime-ms": 136251, "PRIME-Offset": "100", "445 AO-IOChannel": "Dev1/ao1", "640 AO-MaxVolts": "5.0000", "PositionIndex": 3, "PRIME-PP 1 ENABLED": "Yes", "COM1-BaudRate": "9600", "640-State": "0", "COM3-Verbose": "1", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "LudlController-TransmissionDelay": "0", "PRIME-FullWellCapacity": "62000", "PRIME-SMARTStreamingEnabled": "No", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "640 Shutter-Description": "NI DAC", "528-Description": "DA device that is used as a shutter", "COM4-Description": "Serial port driver (boost:asio)", "405 Shutter-Description": "NI DAC", "TE2000-Name": "TE2000", "528 Shutter-Description": "NI DAC", "COM9-Description": "Serial port driver (boost:asio)", "LudlController-Name": "LudlController", "445 Shutter-TriggerSequenceLength": "1000", "PRIME-SerialNumber": "A18B203008", "445 AO-Volts": "0.0000", "PRIME-X-dimension": "1200", "PRIME-ShutterCloseDelay": "0", "XLIGHT Disk slider-State": "2", "Position": "Default", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 AO-IOChannel": "Dev1/ao5", "520 AO-IOChannel": "Dev1/ao4", "528 AO-TriggerSequenceLength": "1000", "405 Shutter-MaxVolts": "5.0000", "470-DA Device": "470 Shutter", "PRIME-FTCapable": "No", "Core-ChannelGroup": "Channels", "555 AO-SupportsTriggering": "0", "PRIME-Y-dimension": "1200", "Frame": 0, "555 Shutter-Sequenceable": "1", "PRIME-Timing-ExposureTimeNs": "9992000.0000", "Core-ImageProcessor": "", "528-State": "0", "520 AO-Block voltage": "false", "XLIGHT Excitation Wheel-Label": "CFP/YFP", "640 Shutter-Demo": "No", "COM3-StopBits": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "520 Shutter-IOChannel": "Dev1/ao11", "Width": 1200, "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-SupportsTriggering": "0", "640 AO-TriggerSampleRate": "100000", "520-Description": "DA device that is used as a shutter", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "405 Shutter-Sequenceable": "1", "405 Shutter-SupportsTriggering": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-TriggerSampleRate": "100000", "640 Shutter-MaxVolts": "5.0000", "555 AO-MaxVolts": "5.0000", "520 AO-TriggerSequenceLength": "1000", "COM3-BaudRate": "9600", "COM9-BaudRate": "9600", "Vincent-D3-Channel #": "Ch. #1", "PRIME-PMode": "Normal", "OpticalPath-Label": "Left(100)", "BitDepth": 16, "555 AO-TriggerSequenceLength": "1000", "445 Shutter-Description": "NI DAC", "405 AO-Sequenceable": "1", "PRIME-AcquisitionMethod": "Callbacks", "555 AO-Name": "AnalogIO", "PRIME-ShutterMode": "Pre-Exposure", "COM3-Handshaking": "Off", "445 AO-SupportsTriggering": "0", "ROI": "0-0-1200-1200", "COM9-Verbose": "1", "PRIME-Color": "OFF", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "PRIME-ShutterOpenDelay": "0", "COM1-Parity": "None", "Slice": 7, "SlicePosition": 20, "COM9-DelayBetweenCharsMs": "0.0000", "555-Name": "DA Shutter", "PIEZO-LudlSingleAxisName": "B", "XLIGHT Emission Wheel-Label": "565/24(G)", "640 Shutter-Name": "AnalogIO", "640 AO-Sequenceable": "1", "520 Shutter-Demo": "No", "528 Shutter-Demo": "No", "470-Name": "DA Shutter", "520-DA Device": "520 Shutter", "XYStage-Description": "Ludl XY stage driver adapter", "445 Shutter-MinVolts": "0.0000", "528 AO-TriggerSampleRate": "100000", "405 AO-MinVolts": "0.0000", "405 Shutter-TriggerSequenceLength": "1000", "Height": 1200, "555 AO-Block voltage": "false", "640 Shutter-Volts": "0.0000", "PixelType": "GRAY16", "445 Shutter-Block voltage": "false", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "520 Shutter-TriggerSequenceLength": "1000", "COM9-Name": "COM9", "Core-SLM": "", "555 AO-Volts": "0.0000", "555 Shutter-Block voltage": "false", "XYStage-Speed": "1800.0000", "520 AO-MaxVolts": "5.0000", "528 Shutter-SupportsTriggering": "0", "COM1-Description": "Serial port driver (boost:asio)", "PRIME-MetadataEnabled": "No", "520 Shutter-MinVolts": "0.0000", "470 Shutter-Sequenceable": "1", "PRIME-PreampDelay": "0", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-MinVolts": "0.0000", "ChannelIndex": 0, "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Core-AutoShutter": "1", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "Exposure-ms": 320, "PRIME-PP 2 ENABLED": "Yes", "640 Shutter-TriggerSampleRate": "100000", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "470 Shutter-IOChannel": "Dev1/ao10", "405 AO-Demo": "No", "PixelSizeUm": 1.1, "470 AO-Demo": "No", "470 Shutter-MaxVolts": "5.0000", "Vincent-D3-Name": "Vincent-D3", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FileName": "img_000000000_Il-G_007.tif", "LudlController-Reset": "Operate", "PRIME-Exposure": "320.00", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "445-Name": "DA Shutter", "555 Shutter-Name": "AnalogIO", "520 Shutter-TriggerSampleRate": "100000", "445 Shutter-IOChannel": "Dev1/ao9", "PRIME-TransposeCorrection": "0", "XYStage-StepSize": "0.1000", "445-Description": "DA device that is used as a shutter", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 AO-IOChannel": "Dev1/ao7", "520 Shutter-Name": "AnalogIO", "COM3-DataBits": "8", "640 AO-Volts": "0.0000", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "555 Shutter-IOChannel": "Dev1/ao13", "WaitInterval": null, "520 Shutter-Sequenceable": "1", "YPositionUm": 20690.300000000003, "445-State": "0", "445-DA Device": "445 Shutter", "PRIME-CircularBufferAutoSize": "ON", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "555 AO-TriggerSampleRate": "100000", "445 Shutter-Volts": "0.0000", "XLIGHT Hub-Port": "COM3", "Core-AutoFocus": "", "COM4-Handshaking": "Off", "528 Shutter-MinVolts": "0.0000", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "445 Shutter-Name": "AnalogIO", "528 Shutter-Sequenceable": "1", "XYStage-TransposeMirrorX": "0", "XYStage-TransposeMirrorY": "0", "PRIME-ReadoutRate": "100MHz 16bit", "XYStage-Name": "Stage", "640 AO-SupportsTriggering": "0", "640-Name": "DA Shutter", "640 Shutter-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "640 AO-Block voltage": "false", "445 Shutter-Sequenceable": "1", "470 AO-MaxVolts": "5.0000", "COM4-BaudRate": "9600", "470-Description": "DA device that is used as a shutter", "520 Shutter-Description": "NI DAC", "PRIME-CCDTemperature": "-14.9600", "Time": "2019-12-17 21:58:43 -0500", "640 AO-TriggerSequenceLength": "1000", "470 AO-Name": "AnalogIO", "XLIGHT Disk slider-Label": "Disk Pos 40um", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "Binning": "1x1", "TE2000-ModelType": "1", "COM3-AnswerTimeout": "500.0000", "555 Shutter-SupportsTriggering": "0", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "TE2000-Port": "COM4", "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Description": "NI DAC", "COM4-AnswerTimeout": "500.0000", "COM1-DelayBetweenCharsMs": "0.0000", "FilterBlock-Label": "Confocal", "445 AO-Name": "AnalogIO", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-Timing-ReadoutTimeNs": "24672000.0000", "528 AO-Demo": "No", "520 AO-Sequenceable": "1", "555-Description": "DA device that is used as a shutter", "405-DA Device": "405 Shutter", "405 AO-TriggerSequenceLength": "1000", "640 AO-MinVolts": "0.0000", "640 Shutter-Block voltage": "false", "FrameIndex": 0, "PRIME-Port": "CMOS", "555 AO-MinVolts": "0.0000", "COM3-Description": "Serial port driver (boost:asio)", "528 AO-Description": "NI DAC", "555 AO-IOChannel": "Dev1/ao6", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "555 AO-TriggerInputLine": "/Dev1/PFI0", "PRIME-ActualInterval-ms": "374.3700", "LudlController-Port": "COM9", "445 Shutter-Demo": "No", "PRIME-Trigger-Expose Out-Mux": "1", "SliceIndex": 7, "PRIME-CCDTemperatureSetPoint": "-15.0000", "405 Shutter-MinVolts": "0.0000", "PRIME-ExposeOutMode": "First Row", "AxisPositions": null, "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Volts": "0.0000", "528-Name": "DA Shutter", "555-State": "0", "XLIGHT Dichroic-Label": "CFP/YFP", "445 AO-Sequenceable": "1", "405-Name": "DA Shutter", "405 AO-IOChannel": "Dev1/ao0", "Core-Camera": "PRIME", "Focus-Name": "Focus", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "405 Shutter-IOChannel": "Dev1/ao8", "640 AO-Demo": "No", "640 Shutter-SupportsTriggering": "0", "445 AO-Demo": "No", "555-DA Device": "555 Shutter", "470 Shutter-Description": "NI DAC", "528 AO-Volts": "0.0000", "COM1-DataBits": "8", "Vincent-D3-Address": "x", "520 AO-SupportsTriggering": "0", "405-Description": "DA device that is used as a shutter", "640 Shutter-MinVolts": "0.0000", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "405 AO-Name": "AnalogIO", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "640 AO-TriggerInputLine": "/Dev1/PFI0", "405 AO-TriggerInputLine": "/Dev1/PFI0", "640-Description": "DA device that is used as a shutter", "445 Shutter-TriggerSampleRate": "100000", "470 Shutter-TriggerSequenceLength": "1000", "COM1-StopBits": "1", "520 Shutter-SupportsTriggering": "0", "405-State": "0", "Core-Initialize": "1", "XLIGHT Emission Wheel-State": "7", "520 AO-Description": "NI DAC", "528 AO-TriggerInputLine": "/Dev1/PFI0", "445 Shutter-MaxVolts": "5.0000", "COM9-Handshaking": "Off", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "XLIGHT Spin motor-Label": "On (spinning)", "PRIME-PP 5 ENABLED": "No", "UUID": "ab8f43db-7e55-48f1-a391-af37fd07edf5", "PRIME-TriggerMode": "Internal Trigger", "COM1-Handshaking": "Off", "PRIME-Timing-PostTriggerDelayNs": "49344000.0000", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "Focus-Position": "16479.8000", "528 Shutter-Block voltage": "false", "Channel": "Il-G", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "555 Shutter-Demo": "No", "405 Shutter-Demo": "No", "528 AO-Name": "AnalogIO", "520-State": "1", "Core-Galvo": "", "405 Shutter-TriggerSampleRate": "100000", "Focus-FocusStepSize_(nm)": "100", "PRIME-PixelType": "16bit", "COM4-DataBits": "8", "470 AO-IOChannel": "Dev1/ao2", "520 AO-MinVolts": "0.0000", "COM9-Parity": "None", "PRIME-CircularBufferEnabled": "ON", "PRIME-PP 6 Reset": "No", "640 AO-Description": "NI DAC", "Source": "PRIME", "445 AO-MinVolts": "0.0000", "PIEZO-Name": "Stage", "445 AO-Description": "NI DAC", "PRIME-CircularBufferFrameCount": "46", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "520 AO-Volts": "4.5000", "528 Shutter-TriggerSampleRate": "100000", "XPositionUm": 62321, "OpticalPath-State": "4", "PRIME-PP 3 THRESHOLD": "80", "PRIME-PVCAM Version": "3.7.1", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Gain": "1-HDR", "640 Shutter-TriggerSequenceLength": "1000", "528 AO-SupportsTriggering": "0", "445 AO-TriggerInputLine": "/Dev1/PFI0", "OpticalPath-Name": "OpticalPath", "PRIME-PP 4 ENABLED": "Yes", "PRIME-PVCAM Adapter Version": "1.3.29", "COM3-Name": "COM3", "405 AO-MaxVolts": "5.0000", "520 Shutter-Block voltage": "false", "PRIME-Timing-ClearingTimeNs": "49344000.0000", "Core-Focus": "PIEZO", "528 AO-Block voltage": "false", "TE2000-Description": "Nikon TE2000 hub", "528 AO-MinVolts": "0.0000", "COM4-Parity": "None", "Summary": { "Slices": 11, "ChContrastMin": [ 0, 0, 0, 0, 0, 0 ], "PixelAspect": 1, "SlicesFirst": true, "Interval_ms": 0, "Time": "2019-12-17 21:58:39 -0500", "Prefix": "seq_3", "InitialPositionList": [ { "Label": "Pos0", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.435], "Focus": [16480.2], "XYStage": [ 69822.40000000001, 20100.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos1", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.425], "Focus": [16480.3], "XYStage": [ 69004, 20101 ] }, "GridColumnIndex": 0 }, { "Label": "Pos2", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.44], "Focus": [16480.1], "XYStage": [ 63119.5, 20690.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos3", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.405], "Focus": [16480.2], "XYStage": [ 62321, 20690.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos4", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.415], "Focus": [16480], "XYStage": [ 56604.100000000006, 20688.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos5", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.42000000000002], "Focus": [16480.1], "XYStage": [ 56034.200000000004, 20688.800000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos6", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.455], "Focus": [16480], "XYStage": [ 50889.3, 20496.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos7", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.43], "Focus": [16480.2], "XYStage": [ 50101.600000000006, 20496.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos8", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.67000000000002], "Focus": [16479.9], "XYStage": [ 44818.600000000006, 20496.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos9", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.67000000000002], "Focus": [16480], "XYStage": [ 43897.9, 20436.800000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos10", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.675], "Focus": [16479.9], "XYStage": [ 39670.600000000006, 20664.9 ] }, "GridColumnIndex": 0 }, { "Label": "Pos11", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.69], "Focus": [16479.9], "XYStage": [ 38734, 20664.600000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos12", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.695], "Focus": [16480.1], "XYStage": [ 34037.200000000004, 20664.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos13", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.685], "Focus": [16480.1], "XYStage": [ 33152, 20664.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos14", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.69], "Focus": [16480.1], "XYStage": [ 28122.800000000003, 20664.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos15", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.675], "Focus": [16480], "XYStage": [ 27422.9, 20664.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos16", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.64000000000001], "Focus": [16480], "XYStage": [ 23005.2, 20664.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos17", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.69], "Focus": [16479.9], "XYStage": [ 21820.600000000002, 20409.600000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos18", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.41], "Focus": [16480.1], "XYStage": [ 73697.6, -13222.800000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos19", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.455], "Focus": [16480.1], "XYStage": [ 72765.8, -13223 ] }, "GridColumnIndex": 0 }, { "Label": "Pos20", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.4], "Focus": [16480.2], "XYStage": [ 67445.5, -12303.800000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos21", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.415], "Focus": [16480.2], "XYStage": [ 66613.3, -12303.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos22", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.415], "Focus": [16480.1], "XYStage": [ 61819.4, -11936.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos23", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.405], "Focus": [16480.2], "XYStage": [ 61325.700000000004, -11937.1 ] }, "GridColumnIndex": 0 }, { "Label": "Pos24", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.46], "Focus": [16480.2], "XYStage": [ 55377, -10974.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos25", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.445], "Focus": [16480.1], "XYStage": [ 54827, -11214.800000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos26", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.435], "Focus": [16480.2], "XYStage": [ 49937.100000000006, -10919.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos27", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.425], "Focus": [16480.1], "XYStage": [ 48764, -10919.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos28", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.42000000000002], "Focus": [16480.2], "XYStage": [ 43978.4, -10920.400000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos29", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.435], "Focus": [16480.2], "XYStage": [ 43106.3, -10920.400000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos30", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.405], "Focus": [16480.3], "XYStage": [ 38212.4, -10920.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos31", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.445], "Focus": [16480.2], "XYStage": [ 37254.9, -10920.400000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos32", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.44], "Focus": [16480.3], "XYStage": [ 32472.800000000003, -11294.400000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos33", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [166.95000000000002], "Focus": [16480.2], "XYStage": [ 31420.7, -11294.400000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos34", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [166.95000000000002], "Focus": [16480.2], "XYStage": [ 26881.5, -11640 ] }, "GridColumnIndex": 0 }, { "Label": "Pos35", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [166.98], "Focus": [16480.2], "XYStage": [ 25876.100000000002, -11641.1 ] }, "GridColumnIndex": 0 } ], "z-step_um": 10, "GridRow": 0, "BitDepth": 16, "Depth": 2, "Channels": 6, "Source": "Micro-Manager", "ChColors": [ -16711681, -256, -65281, -1, -1, -16776961 ], "MetadataVersion": 10, "KeepShutterOpenChannels": true, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536, 65536 ], "CustomIntervals_ms": [], "Height": 1200, "MicroManagerVersion": "1.4.23 20180219", "GridColumn": 0, "UUID": "8c8f9d20-b128-4577-9f91-89e70f22d319", "PixelType": "GRAY16", "Width": 1200, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\Yan\\20191217-YC113-S5S6", "Frames": 1, "Comment": "", "UserName": "Nikon", "PixelSize_um": 1.1, "ROI": [ 0, 0, 1200, 1200 ], "Date": "2019-12-17", "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC", "P-DAPI-high" ], "KeepShutterOpenSlices": true, "IJType": 1, "ComputerName": "ZadorConfocal2", "Positions": 36, "PositionIndex": 3, "CameraTimeout": 20000, "TimeFirst": false }, "640 AO-Name": "AnalogIO", "Vincent-D3-Command": "Close" }, "FrameKey-0-0-8": { "COM4-StopBits": "1", "528 AO-MaxVolts": "5.0000", "XLIGHT Excitation Wheel-State": "2", "470 Shutter-Name": "AnalogIO", "Core-Shutter": "520", "555 AO-Demo": "No", "XLIGHT Dichroic-State": "2", "XYStage-StepSize-Y": "0.1000", "ZPositionUm": 207.405, "XYStage-StepSize-X": "0.1000", "PRIME-FirmwareVersion": "20.28", "PRIME-ClearCycles": "2", "520-Name": "DA Shutter", "405 Shutter-Name": "AnalogIO", "PIEZO-StepSize": "0.0050", "PRIME-ClearMode": "Pre-Exposure", "470 AO-MinVolts": "0.0000", "405 AO-SupportsTriggering": "1", "CameraChannelIndex": 0, "XYStage-StartSpeed": "500.0000", "COM9-StopBits": "2", "405 AO-Description": "NI DAC", "COM4-DelayBetweenCharsMs": "0.0000", "445 AO-Block voltage": "false", "520 AO-TriggerInputLine": "/Dev1/PFI0", "555 AO-Description": "NI DAC", "520 AO-TriggerSampleRate": "100000", "COM4-Verbose": "1", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM4-Name": "COM4", "405 Shutter-Volts": "0.0000", "Vincent-D3-Port": "COM1", "PRIME-PP 4 MIN ADU AFFECTED": "200", "470 Shutter-MinVolts": "0.0000", "PRIME-PP 3 ENABLED": "Yes", "470 AO-Description": "NI DAC", "PRIME-Description": "PVCAM API device adapter", "520 Shutter-MaxVolts": "5.0000", "520 Shutter-Volts": "5.0000", "528 AO-Sequenceable": "1", "PRIME-Binning": "1x1", "555 Shutter-MaxVolts": "5.0000", "528 Shutter-TriggerSequenceLength": "1000", "405 Shutter-Block voltage": "false", "XLIGHT Spin motor-State": "1", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "Vincent-D3-Time to open (ms)": "35.0000", "Vincent-D3-Time to close (ms)": "35.0000", "445 AO-TriggerSampleRate": "100000", "640 Shutter-IOChannel": "Dev1/ao14", "555 AO-Sequenceable": "1", "520 AO-Name": "AnalogIO", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "470 AO-Block voltage": "false", "COM1-Verbose": "1", "COM9-AnswerTimeout": "10000.0000", "528 Shutter-Name": "AnalogIO", "PRIME-Trigger Timeout (secs)": "10", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "NextFrame": 0, "470 AO-TriggerSequenceLength": "1000", "PRIME-TransposeXY": "0", "445 AO-TriggerSequenceLength": "1000", "COM1-Name": "COM1", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "COM9-DataBits": "8", "COM1-AnswerTimeout": "500.0000", "Camera": "", "TE2000-Version": "V2.50-V2.50-V2.10", "528 Shutter-Volts": "0.0000", "XLIGHT Hub-Name": "XLIGHT Hub", "PIEZO-Description": "Ludl stage driver adapter", "PositionName": "Pos3", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSampleRate": "100000", "PRIME-FanSpeedSetpoint": "High", "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "405 AO-TriggerSampleRate": "100000", "470 Shutter-Block voltage": "false", "470-State": "0", "COM3-Parity": "None", "405 AO-Volts": "0.0000", "PRIME-TransposeMirrorY": "0", "PRIME-ChipName": "GS144BSI", "470 AO-Volts": "0.0000", "PRIME-TransposeMirrorX": "0", "445 Shutter-SupportsTriggering": "0", "470 Shutter-SupportsTriggering": "0", "XLIGHT Hub-Description": "XLIGHT hub", "PRIME-PreampOffLimit": "10000", "470 AO-Sequenceable": "1", "528 Shutter-MaxVolts": "5.0000", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "Core-XYStage": "XYStage", "470 AO-TriggerSampleRate": "100000", "PRIME-Timing-PreTriggerDelayNs": "24672000.0000", "528-DA Device": "528 Shutter", "Focus-Description": "Nikon TE2000 focus adapter", "FilterBlock-State": "5", "520 AO-Demo": "No", "COM3-DelayBetweenCharsMs": "0.0000", "470 Shutter-Volts": "0.0000", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "PRIME-PP 2 THRESHOLD": "125", "PRIME-PP 4 THRESHOLD": "75", "XYStage-Acceleration": "4.0000", "470 Shutter-Demo": "No", "PRIME-Name": "PMPCIECam00", "640-DA Device": "640 Shutter", "405 AO-Block voltage": "false", "ElapsedTime-ms": 136810, "PRIME-Offset": "100", "445 AO-IOChannel": "Dev1/ao1", "640 AO-MaxVolts": "5.0000", "PositionIndex": 3, "PRIME-PP 1 ENABLED": "Yes", "COM1-BaudRate": "9600", "640-State": "0", "COM3-Verbose": "1", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "LudlController-TransmissionDelay": "0", "PRIME-FullWellCapacity": "62000", "PRIME-SMARTStreamingEnabled": "No", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "640 Shutter-Description": "NI DAC", "528-Description": "DA device that is used as a shutter", "COM4-Description": "Serial port driver (boost:asio)", "405 Shutter-Description": "NI DAC", "TE2000-Name": "TE2000", "528 Shutter-Description": "NI DAC", "COM9-Description": "Serial port driver (boost:asio)", "LudlController-Name": "LudlController", "445 Shutter-TriggerSequenceLength": "1000", "PRIME-SerialNumber": "A18B203008", "445 AO-Volts": "0.0000", "PRIME-X-dimension": "1200", "PRIME-ShutterCloseDelay": "0", "XLIGHT Disk slider-State": "2", "Position": "Default", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 AO-IOChannel": "Dev1/ao5", "520 AO-IOChannel": "Dev1/ao4", "528 AO-TriggerSequenceLength": "1000", "405 Shutter-MaxVolts": "5.0000", "470-DA Device": "470 Shutter", "PRIME-FTCapable": "No", "Core-ChannelGroup": "Channels", "555 AO-SupportsTriggering": "0", "PRIME-Y-dimension": "1200", "Frame": 0, "555 Shutter-Sequenceable": "1", "PRIME-Timing-ExposureTimeNs": "9992000.0000", "Core-ImageProcessor": "", "528-State": "0", "520 AO-Block voltage": "false", "XLIGHT Excitation Wheel-Label": "CFP/YFP", "640 Shutter-Demo": "No", "COM3-StopBits": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "520 Shutter-IOChannel": "Dev1/ao11", "Width": 1200, "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-SupportsTriggering": "0", "640 AO-TriggerSampleRate": "100000", "520-Description": "DA device that is used as a shutter", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "405 Shutter-Sequenceable": "1", "405 Shutter-SupportsTriggering": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-TriggerSampleRate": "100000", "640 Shutter-MaxVolts": "5.0000", "555 AO-MaxVolts": "5.0000", "520 AO-TriggerSequenceLength": "1000", "COM3-BaudRate": "9600", "COM9-BaudRate": "9600", "Vincent-D3-Channel #": "Ch. #1", "PRIME-PMode": "Normal", "OpticalPath-Label": "Left(100)", "BitDepth": 16, "555 AO-TriggerSequenceLength": "1000", "445 Shutter-Description": "NI DAC", "405 AO-Sequenceable": "1", "PRIME-AcquisitionMethod": "Callbacks", "555 AO-Name": "AnalogIO", "PRIME-ShutterMode": "Pre-Exposure", "COM3-Handshaking": "Off", "445 AO-SupportsTriggering": "0", "ROI": "0-0-1200-1200", "COM9-Verbose": "1", "PRIME-Color": "OFF", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "PRIME-ShutterOpenDelay": "0", "COM1-Parity": "None", "Slice": 8, "SlicePosition": 30, "COM9-DelayBetweenCharsMs": "0.0000", "555-Name": "DA Shutter", "PIEZO-LudlSingleAxisName": "B", "XLIGHT Emission Wheel-Label": "565/24(G)", "640 Shutter-Name": "AnalogIO", "640 AO-Sequenceable": "1", "520 Shutter-Demo": "No", "528 Shutter-Demo": "No", "470-Name": "DA Shutter", "520-DA Device": "520 Shutter", "XYStage-Description": "Ludl XY stage driver adapter", "445 Shutter-MinVolts": "0.0000", "528 AO-TriggerSampleRate": "100000", "405 AO-MinVolts": "0.0000", "405 Shutter-TriggerSequenceLength": "1000", "Height": 1200, "555 AO-Block voltage": "false", "640 Shutter-Volts": "0.0000", "PixelType": "GRAY16", "445 Shutter-Block voltage": "false", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "520 Shutter-TriggerSequenceLength": "1000", "COM9-Name": "COM9", "Core-SLM": "", "555 AO-Volts": "0.0000", "555 Shutter-Block voltage": "false", "XYStage-Speed": "1800.0000", "520 AO-MaxVolts": "5.0000", "528 Shutter-SupportsTriggering": "0", "COM1-Description": "Serial port driver (boost:asio)", "PRIME-MetadataEnabled": "No", "520 Shutter-MinVolts": "0.0000", "470 Shutter-Sequenceable": "1", "PRIME-PreampDelay": "0", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-MinVolts": "0.0000", "ChannelIndex": 0, "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Core-AutoShutter": "1", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "Exposure-ms": 320, "PRIME-PP 2 ENABLED": "Yes", "640 Shutter-TriggerSampleRate": "100000", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "470 Shutter-IOChannel": "Dev1/ao10", "405 AO-Demo": "No", "PixelSizeUm": 1.1, "470 AO-Demo": "No", "470 Shutter-MaxVolts": "5.0000", "Vincent-D3-Name": "Vincent-D3", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FileName": "img_000000000_Il-G_008.tif", "LudlController-Reset": "Operate", "PRIME-Exposure": "320.00", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "445-Name": "DA Shutter", "555 Shutter-Name": "AnalogIO", "520 Shutter-TriggerSampleRate": "100000", "445 Shutter-IOChannel": "Dev1/ao9", "PRIME-TransposeCorrection": "0", "XYStage-StepSize": "0.1000", "445-Description": "DA device that is used as a shutter", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 AO-IOChannel": "Dev1/ao7", "520 Shutter-Name": "AnalogIO", "COM3-DataBits": "8", "640 AO-Volts": "0.0000", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "555 Shutter-IOChannel": "Dev1/ao13", "WaitInterval": null, "520 Shutter-Sequenceable": "1", "YPositionUm": 20690.300000000003, "445-State": "0", "445-DA Device": "445 Shutter", "PRIME-CircularBufferAutoSize": "ON", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "555 AO-TriggerSampleRate": "100000", "445 Shutter-Volts": "0.0000", "XLIGHT Hub-Port": "COM3", "Core-AutoFocus": "", "COM4-Handshaking": "Off", "528 Shutter-MinVolts": "0.0000", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "445 Shutter-Name": "AnalogIO", "528 Shutter-Sequenceable": "1", "XYStage-TransposeMirrorX": "0", "XYStage-TransposeMirrorY": "0", "PRIME-ReadoutRate": "100MHz 16bit", "XYStage-Name": "Stage", "640 AO-SupportsTriggering": "0", "640-Name": "DA Shutter", "640 Shutter-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "640 AO-Block voltage": "false", "445 Shutter-Sequenceable": "1", "470 AO-MaxVolts": "5.0000", "COM4-BaudRate": "9600", "470-Description": "DA device that is used as a shutter", "520 Shutter-Description": "NI DAC", "PRIME-CCDTemperature": "-14.9600", "Time": "2019-12-17 21:58:44 -0500", "640 AO-TriggerSequenceLength": "1000", "470 AO-Name": "AnalogIO", "XLIGHT Disk slider-Label": "Disk Pos 40um", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "Binning": "1x1", "TE2000-ModelType": "1", "COM3-AnswerTimeout": "500.0000", "555 Shutter-SupportsTriggering": "0", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "TE2000-Port": "COM4", "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Description": "NI DAC", "COM4-AnswerTimeout": "500.0000", "COM1-DelayBetweenCharsMs": "0.0000", "FilterBlock-Label": "Confocal", "445 AO-Name": "AnalogIO", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-Timing-ReadoutTimeNs": "24672000.0000", "528 AO-Demo": "No", "520 AO-Sequenceable": "1", "555-Description": "DA device that is used as a shutter", "405-DA Device": "405 Shutter", "405 AO-TriggerSequenceLength": "1000", "640 AO-MinVolts": "0.0000", "640 Shutter-Block voltage": "false", "FrameIndex": 0, "PRIME-Port": "CMOS", "555 AO-MinVolts": "0.0000", "COM3-Description": "Serial port driver (boost:asio)", "528 AO-Description": "NI DAC", "555 AO-IOChannel": "Dev1/ao6", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "555 AO-TriggerInputLine": "/Dev1/PFI0", "PRIME-ActualInterval-ms": "374.3700", "LudlController-Port": "COM9", "445 Shutter-Demo": "No", "PRIME-Trigger-Expose Out-Mux": "1", "SliceIndex": 8, "PRIME-CCDTemperatureSetPoint": "-15.0000", "405 Shutter-MinVolts": "0.0000", "PRIME-ExposeOutMode": "First Row", "AxisPositions": null, "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Volts": "0.0000", "528-Name": "DA Shutter", "555-State": "0", "XLIGHT Dichroic-Label": "CFP/YFP", "445 AO-Sequenceable": "1", "405-Name": "DA Shutter", "405 AO-IOChannel": "Dev1/ao0", "Core-Camera": "PRIME", "Focus-Name": "Focus", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "405 Shutter-IOChannel": "Dev1/ao8", "640 AO-Demo": "No", "640 Shutter-SupportsTriggering": "0", "445 AO-Demo": "No", "555-DA Device": "555 Shutter", "470 Shutter-Description": "NI DAC", "528 AO-Volts": "0.0000", "COM1-DataBits": "8", "Vincent-D3-Address": "x", "520 AO-SupportsTriggering": "0", "405-Description": "DA device that is used as a shutter", "640 Shutter-MinVolts": "0.0000", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "405 AO-Name": "AnalogIO", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "640 AO-TriggerInputLine": "/Dev1/PFI0", "405 AO-TriggerInputLine": "/Dev1/PFI0", "640-Description": "DA device that is used as a shutter", "445 Shutter-TriggerSampleRate": "100000", "470 Shutter-TriggerSequenceLength": "1000", "COM1-StopBits": "1", "520 Shutter-SupportsTriggering": "0", "405-State": "0", "Core-Initialize": "1", "XLIGHT Emission Wheel-State": "7", "520 AO-Description": "NI DAC", "528 AO-TriggerInputLine": "/Dev1/PFI0", "445 Shutter-MaxVolts": "5.0000", "COM9-Handshaking": "Off", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "XLIGHT Spin motor-Label": "On (spinning)", "PRIME-PP 5 ENABLED": "No", "UUID": "be58bdc0-9ec0-4c98-8aa7-73a7e84287a5", "PRIME-TriggerMode": "Internal Trigger", "COM1-Handshaking": "Off", "PRIME-Timing-PostTriggerDelayNs": "49344000.0000", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "Focus-Position": "16479.8000", "528 Shutter-Block voltage": "false", "Channel": "Il-G", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "555 Shutter-Demo": "No", "405 Shutter-Demo": "No", "528 AO-Name": "AnalogIO", "520-State": "1", "Core-Galvo": "", "405 Shutter-TriggerSampleRate": "100000", "Focus-FocusStepSize_(nm)": "100", "PRIME-PixelType": "16bit", "COM4-DataBits": "8", "470 AO-IOChannel": "Dev1/ao2", "520 AO-MinVolts": "0.0000", "COM9-Parity": "None", "PRIME-CircularBufferEnabled": "ON", "PRIME-PP 6 Reset": "No", "640 AO-Description": "NI DAC", "Source": "PRIME", "445 AO-MinVolts": "0.0000", "PIEZO-Name": "Stage", "445 AO-Description": "NI DAC", "PRIME-CircularBufferFrameCount": "46", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "520 AO-Volts": "4.5000", "528 Shutter-TriggerSampleRate": "100000", "XPositionUm": 62321, "OpticalPath-State": "4", "PRIME-PP 3 THRESHOLD": "80", "PRIME-PVCAM Version": "3.7.1", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Gain": "1-HDR", "640 Shutter-TriggerSequenceLength": "1000", "528 AO-SupportsTriggering": "0", "445 AO-TriggerInputLine": "/Dev1/PFI0", "OpticalPath-Name": "OpticalPath", "PRIME-PP 4 ENABLED": "Yes", "PRIME-PVCAM Adapter Version": "1.3.29", "COM3-Name": "COM3", "405 AO-MaxVolts": "5.0000", "520 Shutter-Block voltage": "false", "PRIME-Timing-ClearingTimeNs": "49344000.0000", "Core-Focus": "PIEZO", "528 AO-Block voltage": "false", "TE2000-Description": "Nikon TE2000 hub", "528 AO-MinVolts": "0.0000", "COM4-Parity": "None", "Summary": { "Slices": 11, "ChContrastMin": [ 0, 0, 0, 0, 0, 0 ], "PixelAspect": 1, "SlicesFirst": true, "Interval_ms": 0, "Time": "2019-12-17 21:58:39 -0500", "Prefix": "seq_3", "InitialPositionList": [ { "Label": "Pos0", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.435], "Focus": [16480.2], "XYStage": [ 69822.40000000001, 20100.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos1", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.425], "Focus": [16480.3], "XYStage": [ 69004, 20101 ] }, "GridColumnIndex": 0 }, { "Label": "Pos2", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.44], "Focus": [16480.1], "XYStage": [ 63119.5, 20690.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos3", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.405], "Focus": [16480.2], "XYStage": [ 62321, 20690.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos4", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.415], "Focus": [16480], "XYStage": [ 56604.100000000006, 20688.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos5", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.42000000000002], "Focus": [16480.1], "XYStage": [ 56034.200000000004, 20688.800000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos6", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.455], "Focus": [16480], "XYStage": [ 50889.3, 20496.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos7", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.43], "Focus": [16480.2], "XYStage": [ 50101.600000000006, 20496.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos8", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.67000000000002], "Focus": [16479.9], "XYStage": [ 44818.600000000006, 20496.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos9", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.67000000000002], "Focus": [16480], "XYStage": [ 43897.9, 20436.800000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos10", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.675], "Focus": [16479.9], "XYStage": [ 39670.600000000006, 20664.9 ] }, "GridColumnIndex": 0 }, { "Label": "Pos11", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.69], "Focus": [16479.9], "XYStage": [ 38734, 20664.600000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos12", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.695], "Focus": [16480.1], "XYStage": [ 34037.200000000004, 20664.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos13", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.685], "Focus": [16480.1], "XYStage": [ 33152, 20664.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos14", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.69], "Focus": [16480.1], "XYStage": [ 28122.800000000003, 20664.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos15", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.675], "Focus": [16480], "XYStage": [ 27422.9, 20664.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos16", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.64000000000001], "Focus": [16480], "XYStage": [ 23005.2, 20664.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos17", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.69], "Focus": [16479.9], "XYStage": [ 21820.600000000002, 20409.600000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos18", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.41], "Focus": [16480.1], "XYStage": [ 73697.6, -13222.800000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos19", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.455], "Focus": [16480.1], "XYStage": [ 72765.8, -13223 ] }, "GridColumnIndex": 0 }, { "Label": "Pos20", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.4], "Focus": [16480.2], "XYStage": [ 67445.5, -12303.800000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos21", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.415], "Focus": [16480.2], "XYStage": [ 66613.3, -12303.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos22", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.415], "Focus": [16480.1], "XYStage": [ 61819.4, -11936.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos23", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.405], "Focus": [16480.2], "XYStage": [ 61325.700000000004, -11937.1 ] }, "GridColumnIndex": 0 }, { "Label": "Pos24", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.46], "Focus": [16480.2], "XYStage": [ 55377, -10974.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos25", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.445], "Focus": [16480.1], "XYStage": [ 54827, -11214.800000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos26", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.435], "Focus": [16480.2], "XYStage": [ 49937.100000000006, -10919.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos27", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.425], "Focus": [16480.1], "XYStage": [ 48764, -10919.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos28", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.42000000000002], "Focus": [16480.2], "XYStage": [ 43978.4, -10920.400000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos29", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.435], "Focus": [16480.2], "XYStage": [ 43106.3, -10920.400000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos30", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.405], "Focus": [16480.3], "XYStage": [ 38212.4, -10920.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos31", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.445], "Focus": [16480.2], "XYStage": [ 37254.9, -10920.400000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos32", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.44], "Focus": [16480.3], "XYStage": [ 32472.800000000003, -11294.400000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos33", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [166.95000000000002], "Focus": [16480.2], "XYStage": [ 31420.7, -11294.400000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos34", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [166.95000000000002], "Focus": [16480.2], "XYStage": [ 26881.5, -11640 ] }, "GridColumnIndex": 0 }, { "Label": "Pos35", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [166.98], "Focus": [16480.2], "XYStage": [ 25876.100000000002, -11641.1 ] }, "GridColumnIndex": 0 } ], "z-step_um": 10, "GridRow": 0, "BitDepth": 16, "Depth": 2, "Channels": 6, "Source": "Micro-Manager", "ChColors": [ -16711681, -256, -65281, -1, -1, -16776961 ], "MetadataVersion": 10, "KeepShutterOpenChannels": true, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536, 65536 ], "CustomIntervals_ms": [], "Height": 1200, "MicroManagerVersion": "1.4.23 20180219", "GridColumn": 0, "UUID": "8c8f9d20-b128-4577-9f91-89e70f22d319", "PixelType": "GRAY16", "Width": 1200, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\Yan\\20191217-YC113-S5S6", "Frames": 1, "Comment": "", "UserName": "Nikon", "PixelSize_um": 1.1, "ROI": [ 0, 0, 1200, 1200 ], "Date": "2019-12-17", "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC", "P-DAPI-high" ], "KeepShutterOpenSlices": true, "IJType": 1, "ComputerName": "ZadorConfocal2", "Positions": 36, "PositionIndex": 3, "CameraTimeout": 20000, "TimeFirst": false }, "640 AO-Name": "AnalogIO", "Vincent-D3-Command": "Close" }, "FrameKey-0-0-9": { "COM4-StopBits": "1", "528 AO-MaxVolts": "5.0000", "XLIGHT Excitation Wheel-State": "2", "470 Shutter-Name": "AnalogIO", "Core-Shutter": "520", "555 AO-Demo": "No", "XLIGHT Dichroic-State": "2", "XYStage-StepSize-Y": "0.1000", "ZPositionUm": 217.405, "XYStage-StepSize-X": "0.1000", "PRIME-FirmwareVersion": "20.28", "PRIME-ClearCycles": "2", "520-Name": "DA Shutter", "405 Shutter-Name": "AnalogIO", "PIEZO-StepSize": "0.0050", "PRIME-ClearMode": "Pre-Exposure", "470 AO-MinVolts": "0.0000", "405 AO-SupportsTriggering": "1", "CameraChannelIndex": 0, "XYStage-StartSpeed": "500.0000", "COM9-StopBits": "2", "405 AO-Description": "NI DAC", "COM4-DelayBetweenCharsMs": "0.0000", "445 AO-Block voltage": "false", "520 AO-TriggerInputLine": "/Dev1/PFI0", "555 AO-Description": "NI DAC", "520 AO-TriggerSampleRate": "100000", "COM4-Verbose": "1", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM4-Name": "COM4", "405 Shutter-Volts": "0.0000", "Vincent-D3-Port": "COM1", "PRIME-PP 4 MIN ADU AFFECTED": "200", "470 Shutter-MinVolts": "0.0000", "PRIME-PP 3 ENABLED": "Yes", "470 AO-Description": "NI DAC", "PRIME-Description": "PVCAM API device adapter", "520 Shutter-MaxVolts": "5.0000", "520 Shutter-Volts": "5.0000", "528 AO-Sequenceable": "1", "PRIME-Binning": "1x1", "555 Shutter-MaxVolts": "5.0000", "528 Shutter-TriggerSequenceLength": "1000", "405 Shutter-Block voltage": "false", "XLIGHT Spin motor-State": "1", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "Vincent-D3-Time to open (ms)": "35.0000", "Vincent-D3-Time to close (ms)": "35.0000", "445 AO-TriggerSampleRate": "100000", "640 Shutter-IOChannel": "Dev1/ao14", "555 AO-Sequenceable": "1", "520 AO-Name": "AnalogIO", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "470 AO-Block voltage": "false", "COM1-Verbose": "1", "COM9-AnswerTimeout": "10000.0000", "528 Shutter-Name": "AnalogIO", "PRIME-Trigger Timeout (secs)": "10", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "NextFrame": 0, "470 AO-TriggerSequenceLength": "1000", "PRIME-TransposeXY": "0", "445 AO-TriggerSequenceLength": "1000", "COM1-Name": "COM1", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "COM9-DataBits": "8", "COM1-AnswerTimeout": "500.0000", "Camera": "", "TE2000-Version": "V2.50-V2.50-V2.10", "528 Shutter-Volts": "0.0000", "XLIGHT Hub-Name": "XLIGHT Hub", "PIEZO-Description": "Ludl stage driver adapter", "PositionName": "Pos3", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSampleRate": "100000", "PRIME-FanSpeedSetpoint": "High", "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "405 AO-TriggerSampleRate": "100000", "470 Shutter-Block voltage": "false", "470-State": "0", "COM3-Parity": "None", "405 AO-Volts": "0.0000", "PRIME-TransposeMirrorY": "0", "PRIME-ChipName": "GS144BSI", "470 AO-Volts": "0.0000", "PRIME-TransposeMirrorX": "0", "445 Shutter-SupportsTriggering": "0", "470 Shutter-SupportsTriggering": "0", "XLIGHT Hub-Description": "XLIGHT hub", "PRIME-PreampOffLimit": "10000", "470 AO-Sequenceable": "1", "528 Shutter-MaxVolts": "5.0000", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "Core-XYStage": "XYStage", "470 AO-TriggerSampleRate": "100000", "PRIME-Timing-PreTriggerDelayNs": "24672000.0000", "528-DA Device": "528 Shutter", "Focus-Description": "Nikon TE2000 focus adapter", "FilterBlock-State": "5", "520 AO-Demo": "No", "COM3-DelayBetweenCharsMs": "0.0000", "470 Shutter-Volts": "0.0000", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "PRIME-PP 2 THRESHOLD": "125", "PRIME-PP 4 THRESHOLD": "75", "XYStage-Acceleration": "4.0000", "470 Shutter-Demo": "No", "PRIME-Name": "PMPCIECam00", "640-DA Device": "640 Shutter", "405 AO-Block voltage": "false", "ElapsedTime-ms": 137372, "PRIME-Offset": "100", "445 AO-IOChannel": "Dev1/ao1", "640 AO-MaxVolts": "5.0000", "PositionIndex": 3, "PRIME-PP 1 ENABLED": "Yes", "COM1-BaudRate": "9600", "640-State": "0", "COM3-Verbose": "1", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "LudlController-TransmissionDelay": "0", "PRIME-FullWellCapacity": "62000", "PRIME-SMARTStreamingEnabled": "No", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "640 Shutter-Description": "NI DAC", "528-Description": "DA device that is used as a shutter", "COM4-Description": "Serial port driver (boost:asio)", "405 Shutter-Description": "NI DAC", "TE2000-Name": "TE2000", "528 Shutter-Description": "NI DAC", "COM9-Description": "Serial port driver (boost:asio)", "LudlController-Name": "LudlController", "445 Shutter-TriggerSequenceLength": "1000", "PRIME-SerialNumber": "A18B203008", "445 AO-Volts": "0.0000", "PRIME-X-dimension": "1200", "PRIME-ShutterCloseDelay": "0", "XLIGHT Disk slider-State": "2", "Position": "Default", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 AO-IOChannel": "Dev1/ao5", "520 AO-IOChannel": "Dev1/ao4", "528 AO-TriggerSequenceLength": "1000", "405 Shutter-MaxVolts": "5.0000", "470-DA Device": "470 Shutter", "PRIME-FTCapable": "No", "Core-ChannelGroup": "Channels", "555 AO-SupportsTriggering": "0", "PRIME-Y-dimension": "1200", "Frame": 0, "555 Shutter-Sequenceable": "1", "PRIME-Timing-ExposureTimeNs": "9992000.0000", "Core-ImageProcessor": "", "528-State": "0", "520 AO-Block voltage": "false", "XLIGHT Excitation Wheel-Label": "CFP/YFP", "640 Shutter-Demo": "No", "COM3-StopBits": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "520 Shutter-IOChannel": "Dev1/ao11", "Width": 1200, "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-SupportsTriggering": "0", "640 AO-TriggerSampleRate": "100000", "520-Description": "DA device that is used as a shutter", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "405 Shutter-Sequenceable": "1", "405 Shutter-SupportsTriggering": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-TriggerSampleRate": "100000", "640 Shutter-MaxVolts": "5.0000", "555 AO-MaxVolts": "5.0000", "520 AO-TriggerSequenceLength": "1000", "COM3-BaudRate": "9600", "COM9-BaudRate": "9600", "Vincent-D3-Channel #": "Ch. #1", "PRIME-PMode": "Normal", "OpticalPath-Label": "Left(100)", "BitDepth": 16, "555 AO-TriggerSequenceLength": "1000", "445 Shutter-Description": "NI DAC", "405 AO-Sequenceable": "1", "PRIME-AcquisitionMethod": "Callbacks", "555 AO-Name": "AnalogIO", "PRIME-ShutterMode": "Pre-Exposure", "COM3-Handshaking": "Off", "445 AO-SupportsTriggering": "0", "ROI": "0-0-1200-1200", "COM9-Verbose": "1", "PRIME-Color": "OFF", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "PRIME-ShutterOpenDelay": "0", "COM1-Parity": "None", "Slice": 9, "SlicePosition": 40, "COM9-DelayBetweenCharsMs": "0.0000", "555-Name": "DA Shutter", "PIEZO-LudlSingleAxisName": "B", "XLIGHT Emission Wheel-Label": "565/24(G)", "640 Shutter-Name": "AnalogIO", "640 AO-Sequenceable": "1", "520 Shutter-Demo": "No", "528 Shutter-Demo": "No", "470-Name": "DA Shutter", "520-DA Device": "520 Shutter", "XYStage-Description": "Ludl XY stage driver adapter", "445 Shutter-MinVolts": "0.0000", "528 AO-TriggerSampleRate": "100000", "405 AO-MinVolts": "0.0000", "405 Shutter-TriggerSequenceLength": "1000", "Height": 1200, "555 AO-Block voltage": "false", "640 Shutter-Volts": "0.0000", "PixelType": "GRAY16", "445 Shutter-Block voltage": "false", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "520 Shutter-TriggerSequenceLength": "1000", "COM9-Name": "COM9", "Core-SLM": "", "555 AO-Volts": "0.0000", "555 Shutter-Block voltage": "false", "XYStage-Speed": "1800.0000", "520 AO-MaxVolts": "5.0000", "528 Shutter-SupportsTriggering": "0", "COM1-Description": "Serial port driver (boost:asio)", "PRIME-MetadataEnabled": "No", "520 Shutter-MinVolts": "0.0000", "470 Shutter-Sequenceable": "1", "PRIME-PreampDelay": "0", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-MinVolts": "0.0000", "ChannelIndex": 0, "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Core-AutoShutter": "1", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "Exposure-ms": 320, "PRIME-PP 2 ENABLED": "Yes", "640 Shutter-TriggerSampleRate": "100000", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "470 Shutter-IOChannel": "Dev1/ao10", "405 AO-Demo": "No", "PixelSizeUm": 1.1, "470 AO-Demo": "No", "470 Shutter-MaxVolts": "5.0000", "Vincent-D3-Name": "Vincent-D3", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FileName": "img_000000000_Il-G_009.tif", "LudlController-Reset": "Operate", "PRIME-Exposure": "320.00", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "445-Name": "DA Shutter", "555 Shutter-Name": "AnalogIO", "520 Shutter-TriggerSampleRate": "100000", "445 Shutter-IOChannel": "Dev1/ao9", "PRIME-TransposeCorrection": "0", "XYStage-StepSize": "0.1000", "445-Description": "DA device that is used as a shutter", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 AO-IOChannel": "Dev1/ao7", "520 Shutter-Name": "AnalogIO", "COM3-DataBits": "8", "640 AO-Volts": "0.0000", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "555 Shutter-IOChannel": "Dev1/ao13", "WaitInterval": null, "520 Shutter-Sequenceable": "1", "YPositionUm": 20690.300000000003, "445-State": "0", "445-DA Device": "445 Shutter", "PRIME-CircularBufferAutoSize": "ON", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "555 AO-TriggerSampleRate": "100000", "445 Shutter-Volts": "0.0000", "XLIGHT Hub-Port": "COM3", "Core-AutoFocus": "", "COM4-Handshaking": "Off", "528 Shutter-MinVolts": "0.0000", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "445 Shutter-Name": "AnalogIO", "528 Shutter-Sequenceable": "1", "XYStage-TransposeMirrorX": "0", "XYStage-TransposeMirrorY": "0", "PRIME-ReadoutRate": "100MHz 16bit", "XYStage-Name": "Stage", "640 AO-SupportsTriggering": "0", "640-Name": "DA Shutter", "640 Shutter-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "640 AO-Block voltage": "false", "445 Shutter-Sequenceable": "1", "470 AO-MaxVolts": "5.0000", "COM4-BaudRate": "9600", "470-Description": "DA device that is used as a shutter", "520 Shutter-Description": "NI DAC", "PRIME-CCDTemperature": "-14.9600", "Time": "2019-12-17 21:58:44 -0500", "640 AO-TriggerSequenceLength": "1000", "470 AO-Name": "AnalogIO", "XLIGHT Disk slider-Label": "Disk Pos 40um", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "Binning": "1x1", "TE2000-ModelType": "1", "COM3-AnswerTimeout": "500.0000", "555 Shutter-SupportsTriggering": "0", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "TE2000-Port": "COM4", "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Description": "NI DAC", "COM4-AnswerTimeout": "500.0000", "COM1-DelayBetweenCharsMs": "0.0000", "FilterBlock-Label": "Confocal", "445 AO-Name": "AnalogIO", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-Timing-ReadoutTimeNs": "24672000.0000", "528 AO-Demo": "No", "520 AO-Sequenceable": "1", "555-Description": "DA device that is used as a shutter", "405-DA Device": "405 Shutter", "405 AO-TriggerSequenceLength": "1000", "640 AO-MinVolts": "0.0000", "640 Shutter-Block voltage": "false", "FrameIndex": 0, "PRIME-Port": "CMOS", "555 AO-MinVolts": "0.0000", "COM3-Description": "Serial port driver (boost:asio)", "528 AO-Description": "NI DAC", "555 AO-IOChannel": "Dev1/ao6", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "555 AO-TriggerInputLine": "/Dev1/PFI0", "PRIME-ActualInterval-ms": "374.3700", "LudlController-Port": "COM9", "445 Shutter-Demo": "No", "PRIME-Trigger-Expose Out-Mux": "1", "SliceIndex": 9, "PRIME-CCDTemperatureSetPoint": "-15.0000", "405 Shutter-MinVolts": "0.0000", "PRIME-ExposeOutMode": "First Row", "AxisPositions": null, "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Volts": "0.0000", "528-Name": "DA Shutter", "555-State": "0", "XLIGHT Dichroic-Label": "CFP/YFP", "445 AO-Sequenceable": "1", "405-Name": "DA Shutter", "405 AO-IOChannel": "Dev1/ao0", "Core-Camera": "PRIME", "Focus-Name": "Focus", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "405 Shutter-IOChannel": "Dev1/ao8", "640 AO-Demo": "No", "640 Shutter-SupportsTriggering": "0", "445 AO-Demo": "No", "555-DA Device": "555 Shutter", "470 Shutter-Description": "NI DAC", "528 AO-Volts": "0.0000", "COM1-DataBits": "8", "Vincent-D3-Address": "x", "520 AO-SupportsTriggering": "0", "405-Description": "DA device that is used as a shutter", "640 Shutter-MinVolts": "0.0000", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "405 AO-Name": "AnalogIO", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "640 AO-TriggerInputLine": "/Dev1/PFI0", "405 AO-TriggerInputLine": "/Dev1/PFI0", "640-Description": "DA device that is used as a shutter", "445 Shutter-TriggerSampleRate": "100000", "470 Shutter-TriggerSequenceLength": "1000", "COM1-StopBits": "1", "520 Shutter-SupportsTriggering": "0", "405-State": "0", "Core-Initialize": "1", "XLIGHT Emission Wheel-State": "7", "520 AO-Description": "NI DAC", "528 AO-TriggerInputLine": "/Dev1/PFI0", "445 Shutter-MaxVolts": "5.0000", "COM9-Handshaking": "Off", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "XLIGHT Spin motor-Label": "On (spinning)", "PRIME-PP 5 ENABLED": "No", "UUID": "5a7ea6fe-88a9-4a0b-84d8-c5764b049bfc", "PRIME-TriggerMode": "Internal Trigger", "COM1-Handshaking": "Off", "PRIME-Timing-PostTriggerDelayNs": "49344000.0000", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "Focus-Position": "16479.8000", "528 Shutter-Block voltage": "false", "Channel": "Il-G", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "555 Shutter-Demo": "No", "405 Shutter-Demo": "No", "528 AO-Name": "AnalogIO", "520-State": "1", "Core-Galvo": "", "405 Shutter-TriggerSampleRate": "100000", "Focus-FocusStepSize_(nm)": "100", "PRIME-PixelType": "16bit", "COM4-DataBits": "8", "470 AO-IOChannel": "Dev1/ao2", "520 AO-MinVolts": "0.0000", "COM9-Parity": "None", "PRIME-CircularBufferEnabled": "ON", "PRIME-PP 6 Reset": "No", "640 AO-Description": "NI DAC", "Source": "PRIME", "445 AO-MinVolts": "0.0000", "PIEZO-Name": "Stage", "445 AO-Description": "NI DAC", "PRIME-CircularBufferFrameCount": "46", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "520 AO-Volts": "4.5000", "528 Shutter-TriggerSampleRate": "100000", "XPositionUm": 62321, "OpticalPath-State": "4", "PRIME-PP 3 THRESHOLD": "80", "PRIME-PVCAM Version": "3.7.1", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Gain": "1-HDR", "640 Shutter-TriggerSequenceLength": "1000", "528 AO-SupportsTriggering": "0", "445 AO-TriggerInputLine": "/Dev1/PFI0", "OpticalPath-Name": "OpticalPath", "PRIME-PP 4 ENABLED": "Yes", "PRIME-PVCAM Adapter Version": "1.3.29", "COM3-Name": "COM3", "405 AO-MaxVolts": "5.0000", "520 Shutter-Block voltage": "false", "PRIME-Timing-ClearingTimeNs": "49344000.0000", "Core-Focus": "PIEZO", "528 AO-Block voltage": "false", "TE2000-Description": "Nikon TE2000 hub", "528 AO-MinVolts": "0.0000", "COM4-Parity": "None", "Summary": { "Slices": 11, "ChContrastMin": [ 0, 0, 0, 0, 0, 0 ], "PixelAspect": 1, "SlicesFirst": true, "Interval_ms": 0, "Time": "2019-12-17 21:58:39 -0500", "Prefix": "seq_3", "InitialPositionList": [ { "Label": "Pos0", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.435], "Focus": [16480.2], "XYStage": [ 69822.40000000001, 20100.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos1", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.425], "Focus": [16480.3], "XYStage": [ 69004, 20101 ] }, "GridColumnIndex": 0 }, { "Label": "Pos2", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.44], "Focus": [16480.1], "XYStage": [ 63119.5, 20690.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos3", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.405], "Focus": [16480.2], "XYStage": [ 62321, 20690.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos4", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.415], "Focus": [16480], "XYStage": [ 56604.100000000006, 20688.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos5", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.42000000000002], "Focus": [16480.1], "XYStage": [ 56034.200000000004, 20688.800000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos6", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.455], "Focus": [16480], "XYStage": [ 50889.3, 20496.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos7", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.43], "Focus": [16480.2], "XYStage": [ 50101.600000000006, 20496.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos8", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.67000000000002], "Focus": [16479.9], "XYStage": [ 44818.600000000006, 20496.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos9", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.67000000000002], "Focus": [16480], "XYStage": [ 43897.9, 20436.800000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos10", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.675], "Focus": [16479.9], "XYStage": [ 39670.600000000006, 20664.9 ] }, "GridColumnIndex": 0 }, { "Label": "Pos11", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.69], "Focus": [16479.9], "XYStage": [ 38734, 20664.600000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos12", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.695], "Focus": [16480.1], "XYStage": [ 34037.200000000004, 20664.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos13", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.685], "Focus": [16480.1], "XYStage": [ 33152, 20664.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos14", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.69], "Focus": [16480.1], "XYStage": [ 28122.800000000003, 20664.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos15", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.675], "Focus": [16480], "XYStage": [ 27422.9, 20664.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos16", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.64000000000001], "Focus": [16480], "XYStage": [ 23005.2, 20664.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos17", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.69], "Focus": [16479.9], "XYStage": [ 21820.600000000002, 20409.600000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos18", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.41], "Focus": [16480.1], "XYStage": [ 73697.6, -13222.800000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos19", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.455], "Focus": [16480.1], "XYStage": [ 72765.8, -13223 ] }, "GridColumnIndex": 0 }, { "Label": "Pos20", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.4], "Focus": [16480.2], "XYStage": [ 67445.5, -12303.800000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos21", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.415], "Focus": [16480.2], "XYStage": [ 66613.3, -12303.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos22", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.415], "Focus": [16480.1], "XYStage": [ 61819.4, -11936.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos23", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.405], "Focus": [16480.2], "XYStage": [ 61325.700000000004, -11937.1 ] }, "GridColumnIndex": 0 }, { "Label": "Pos24", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.46], "Focus": [16480.2], "XYStage": [ 55377, -10974.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos25", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.445], "Focus": [16480.1], "XYStage": [ 54827, -11214.800000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos26", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.435], "Focus": [16480.2], "XYStage": [ 49937.100000000006, -10919.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos27", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.425], "Focus": [16480.1], "XYStage": [ 48764, -10919.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos28", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.42000000000002], "Focus": [16480.2], "XYStage": [ 43978.4, -10920.400000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos29", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.435], "Focus": [16480.2], "XYStage": [ 43106.3, -10920.400000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos30", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.405], "Focus": [16480.3], "XYStage": [ 38212.4, -10920.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos31", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.445], "Focus": [16480.2], "XYStage": [ 37254.9, -10920.400000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos32", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.44], "Focus": [16480.3], "XYStage": [ 32472.800000000003, -11294.400000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos33", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [166.95000000000002], "Focus": [16480.2], "XYStage": [ 31420.7, -11294.400000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos34", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [166.95000000000002], "Focus": [16480.2], "XYStage": [ 26881.5, -11640 ] }, "GridColumnIndex": 0 }, { "Label": "Pos35", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [166.98], "Focus": [16480.2], "XYStage": [ 25876.100000000002, -11641.1 ] }, "GridColumnIndex": 0 } ], "z-step_um": 10, "GridRow": 0, "BitDepth": 16, "Depth": 2, "Channels": 6, "Source": "Micro-Manager", "ChColors": [ -16711681, -256, -65281, -1, -1, -16776961 ], "MetadataVersion": 10, "KeepShutterOpenChannels": true, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536, 65536 ], "CustomIntervals_ms": [], "Height": 1200, "MicroManagerVersion": "1.4.23 20180219", "GridColumn": 0, "UUID": "8c8f9d20-b128-4577-9f91-89e70f22d319", "PixelType": "GRAY16", "Width": 1200, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\Yan\\20191217-YC113-S5S6", "Frames": 1, "Comment": "", "UserName": "Nikon", "PixelSize_um": 1.1, "ROI": [ 0, 0, 1200, 1200 ], "Date": "2019-12-17", "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC", "P-DAPI-high" ], "KeepShutterOpenSlices": true, "IJType": 1, "ComputerName": "ZadorConfocal2", "Positions": 36, "PositionIndex": 3, "CameraTimeout": 20000, "TimeFirst": false }, "640 AO-Name": "AnalogIO", "Vincent-D3-Command": "Close" }, "FrameKey-0-0-10": { "COM4-StopBits": "1", "528 AO-MaxVolts": "5.0000", "XLIGHT Excitation Wheel-State": "2", "470 Shutter-Name": "AnalogIO", "Core-Shutter": "520", "555 AO-Demo": "No", "XLIGHT Dichroic-State": "2", "XYStage-StepSize-Y": "0.1000", "ZPositionUm": 227.405, "XYStage-StepSize-X": "0.1000", "PRIME-FirmwareVersion": "20.28", "PRIME-ClearCycles": "2", "520-Name": "DA Shutter", "405 Shutter-Name": "AnalogIO", "PIEZO-StepSize": "0.0050", "PRIME-ClearMode": "Pre-Exposure", "470 AO-MinVolts": "0.0000", "405 AO-SupportsTriggering": "1", "CameraChannelIndex": 0, "XYStage-StartSpeed": "500.0000", "COM9-StopBits": "2", "405 AO-Description": "NI DAC", "COM4-DelayBetweenCharsMs": "0.0000", "445 AO-Block voltage": "false", "520 AO-TriggerInputLine": "/Dev1/PFI0", "555 AO-Description": "NI DAC", "520 AO-TriggerSampleRate": "100000", "COM4-Verbose": "1", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM4-Name": "COM4", "405 Shutter-Volts": "0.0000", "Vincent-D3-Port": "COM1", "PRIME-PP 4 MIN ADU AFFECTED": "200", "470 Shutter-MinVolts": "0.0000", "PRIME-PP 3 ENABLED": "Yes", "470 AO-Description": "NI DAC", "PRIME-Description": "PVCAM API device adapter", "520 Shutter-MaxVolts": "5.0000", "520 Shutter-Volts": "5.0000", "528 AO-Sequenceable": "1", "PRIME-Binning": "1x1", "555 Shutter-MaxVolts": "5.0000", "528 Shutter-TriggerSequenceLength": "1000", "405 Shutter-Block voltage": "false", "XLIGHT Spin motor-State": "1", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "Vincent-D3-Time to open (ms)": "35.0000", "Vincent-D3-Time to close (ms)": "35.0000", "445 AO-TriggerSampleRate": "100000", "640 Shutter-IOChannel": "Dev1/ao14", "555 AO-Sequenceable": "1", "520 AO-Name": "AnalogIO", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "470 AO-Block voltage": "false", "COM1-Verbose": "1", "COM9-AnswerTimeout": "10000.0000", "528 Shutter-Name": "AnalogIO", "PRIME-Trigger Timeout (secs)": "10", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "NextFrame": 0, "470 AO-TriggerSequenceLength": "1000", "PRIME-TransposeXY": "0", "445 AO-TriggerSequenceLength": "1000", "COM1-Name": "COM1", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "COM9-DataBits": "8", "COM1-AnswerTimeout": "500.0000", "Camera": "", "TE2000-Version": "V2.50-V2.50-V2.10", "528 Shutter-Volts": "0.0000", "XLIGHT Hub-Name": "XLIGHT Hub", "PIEZO-Description": "Ludl stage driver adapter", "PositionName": "Pos3", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSampleRate": "100000", "PRIME-FanSpeedSetpoint": "High", "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "405 AO-TriggerSampleRate": "100000", "470 Shutter-Block voltage": "false", "470-State": "0", "COM3-Parity": "None", "405 AO-Volts": "0.0000", "PRIME-TransposeMirrorY": "0", "PRIME-ChipName": "GS144BSI", "470 AO-Volts": "0.0000", "PRIME-TransposeMirrorX": "0", "445 Shutter-SupportsTriggering": "0", "470 Shutter-SupportsTriggering": "0", "XLIGHT Hub-Description": "XLIGHT hub", "PRIME-PreampOffLimit": "10000", "470 AO-Sequenceable": "1", "528 Shutter-MaxVolts": "5.0000", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "Core-XYStage": "XYStage", "470 AO-TriggerSampleRate": "100000", "PRIME-Timing-PreTriggerDelayNs": "24672000.0000", "528-DA Device": "528 Shutter", "Focus-Description": "Nikon TE2000 focus adapter", "FilterBlock-State": "5", "520 AO-Demo": "No", "COM3-DelayBetweenCharsMs": "0.0000", "470 Shutter-Volts": "0.0000", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "PRIME-PP 2 THRESHOLD": "125", "PRIME-PP 4 THRESHOLD": "75", "XYStage-Acceleration": "4.0000", "470 Shutter-Demo": "No", "PRIME-Name": "PMPCIECam00", "640-DA Device": "640 Shutter", "405 AO-Block voltage": "false", "ElapsedTime-ms": 137931, "PRIME-Offset": "100", "445 AO-IOChannel": "Dev1/ao1", "640 AO-MaxVolts": "5.0000", "PositionIndex": 3, "PRIME-PP 1 ENABLED": "Yes", "COM1-BaudRate": "9600", "640-State": "0", "COM3-Verbose": "1", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "LudlController-TransmissionDelay": "0", "PRIME-FullWellCapacity": "62000", "PRIME-SMARTStreamingEnabled": "No", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "640 Shutter-Description": "NI DAC", "528-Description": "DA device that is used as a shutter", "COM4-Description": "Serial port driver (boost:asio)", "405 Shutter-Description": "NI DAC", "TE2000-Name": "TE2000", "528 Shutter-Description": "NI DAC", "COM9-Description": "Serial port driver (boost:asio)", "LudlController-Name": "LudlController", "445 Shutter-TriggerSequenceLength": "1000", "PRIME-SerialNumber": "A18B203008", "445 AO-Volts": "0.0000", "PRIME-X-dimension": "1200", "PRIME-ShutterCloseDelay": "0", "XLIGHT Disk slider-State": "2", "Position": "Default", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 AO-IOChannel": "Dev1/ao5", "520 AO-IOChannel": "Dev1/ao4", "528 AO-TriggerSequenceLength": "1000", "405 Shutter-MaxVolts": "5.0000", "470-DA Device": "470 Shutter", "PRIME-FTCapable": "No", "Core-ChannelGroup": "Channels", "555 AO-SupportsTriggering": "0", "PRIME-Y-dimension": "1200", "Frame": 0, "555 Shutter-Sequenceable": "1", "PRIME-Timing-ExposureTimeNs": "9992000.0000", "Core-ImageProcessor": "", "528-State": "0", "520 AO-Block voltage": "false", "XLIGHT Excitation Wheel-Label": "CFP/YFP", "640 Shutter-Demo": "No", "COM3-StopBits": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "520 Shutter-IOChannel": "Dev1/ao11", "Width": 1200, "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-SupportsTriggering": "0", "640 AO-TriggerSampleRate": "100000", "520-Description": "DA device that is used as a shutter", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "405 Shutter-Sequenceable": "1", "405 Shutter-SupportsTriggering": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-TriggerSampleRate": "100000", "640 Shutter-MaxVolts": "5.0000", "555 AO-MaxVolts": "5.0000", "520 AO-TriggerSequenceLength": "1000", "COM3-BaudRate": "9600", "COM9-BaudRate": "9600", "Vincent-D3-Channel #": "Ch. #1", "PRIME-PMode": "Normal", "OpticalPath-Label": "Left(100)", "BitDepth": 16, "555 AO-TriggerSequenceLength": "1000", "445 Shutter-Description": "NI DAC", "405 AO-Sequenceable": "1", "PRIME-AcquisitionMethod": "Callbacks", "555 AO-Name": "AnalogIO", "PRIME-ShutterMode": "Pre-Exposure", "COM3-Handshaking": "Off", "445 AO-SupportsTriggering": "0", "ROI": "0-0-1200-1200", "COM9-Verbose": "1", "PRIME-Color": "OFF", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "PRIME-ShutterOpenDelay": "0", "COM1-Parity": "None", "Slice": 10, "SlicePosition": 50, "COM9-DelayBetweenCharsMs": "0.0000", "555-Name": "DA Shutter", "PIEZO-LudlSingleAxisName": "B", "XLIGHT Emission Wheel-Label": "565/24(G)", "640 Shutter-Name": "AnalogIO", "640 AO-Sequenceable": "1", "520 Shutter-Demo": "No", "528 Shutter-Demo": "No", "470-Name": "DA Shutter", "520-DA Device": "520 Shutter", "XYStage-Description": "Ludl XY stage driver adapter", "445 Shutter-MinVolts": "0.0000", "528 AO-TriggerSampleRate": "100000", "405 AO-MinVolts": "0.0000", "405 Shutter-TriggerSequenceLength": "1000", "Height": 1200, "555 AO-Block voltage": "false", "640 Shutter-Volts": "0.0000", "PixelType": "GRAY16", "445 Shutter-Block voltage": "false", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "520 Shutter-TriggerSequenceLength": "1000", "COM9-Name": "COM9", "Core-SLM": "", "555 AO-Volts": "0.0000", "555 Shutter-Block voltage": "false", "XYStage-Speed": "1800.0000", "520 AO-MaxVolts": "5.0000", "528 Shutter-SupportsTriggering": "0", "COM1-Description": "Serial port driver (boost:asio)", "PRIME-MetadataEnabled": "No", "520 Shutter-MinVolts": "0.0000", "470 Shutter-Sequenceable": "1", "PRIME-PreampDelay": "0", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-MinVolts": "0.0000", "ChannelIndex": 0, "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Core-AutoShutter": "1", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "Exposure-ms": 320, "PRIME-PP 2 ENABLED": "Yes", "640 Shutter-TriggerSampleRate": "100000", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "470 Shutter-IOChannel": "Dev1/ao10", "405 AO-Demo": "No", "PixelSizeUm": 1.1, "470 AO-Demo": "No", "470 Shutter-MaxVolts": "5.0000", "Vincent-D3-Name": "Vincent-D3", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FileName": "img_000000000_Il-G_010.tif", "LudlController-Reset": "Operate", "PRIME-Exposure": "320.00", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "445-Name": "DA Shutter", "555 Shutter-Name": "AnalogIO", "520 Shutter-TriggerSampleRate": "100000", "445 Shutter-IOChannel": "Dev1/ao9", "PRIME-TransposeCorrection": "0", "XYStage-StepSize": "0.1000", "445-Description": "DA device that is used as a shutter", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 AO-IOChannel": "Dev1/ao7", "520 Shutter-Name": "AnalogIO", "COM3-DataBits": "8", "640 AO-Volts": "0.0000", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "555 Shutter-IOChannel": "Dev1/ao13", "WaitInterval": null, "520 Shutter-Sequenceable": "1", "YPositionUm": 20690.300000000003, "445-State": "0", "445-DA Device": "445 Shutter", "PRIME-CircularBufferAutoSize": "ON", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "555 AO-TriggerSampleRate": "100000", "445 Shutter-Volts": "0.0000", "XLIGHT Hub-Port": "COM3", "Core-AutoFocus": "", "COM4-Handshaking": "Off", "528 Shutter-MinVolts": "0.0000", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "445 Shutter-Name": "AnalogIO", "528 Shutter-Sequenceable": "1", "XYStage-TransposeMirrorX": "0", "XYStage-TransposeMirrorY": "0", "PRIME-ReadoutRate": "100MHz 16bit", "XYStage-Name": "Stage", "640 AO-SupportsTriggering": "0", "640-Name": "DA Shutter", "640 Shutter-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "640 AO-Block voltage": "false", "445 Shutter-Sequenceable": "1", "470 AO-MaxVolts": "5.0000", "COM4-BaudRate": "9600", "470-Description": "DA device that is used as a shutter", "520 Shutter-Description": "NI DAC", "PRIME-CCDTemperature": "-14.9600", "Time": "2019-12-17 21:58:45 -0500", "640 AO-TriggerSequenceLength": "1000", "470 AO-Name": "AnalogIO", "XLIGHT Disk slider-Label": "Disk Pos 40um", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "Binning": "1x1", "TE2000-ModelType": "1", "COM3-AnswerTimeout": "500.0000", "555 Shutter-SupportsTriggering": "0", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "TE2000-Port": "COM4", "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Description": "NI DAC", "COM4-AnswerTimeout": "500.0000", "COM1-DelayBetweenCharsMs": "0.0000", "FilterBlock-Label": "Confocal", "445 AO-Name": "AnalogIO", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-Timing-ReadoutTimeNs": "24672000.0000", "528 AO-Demo": "No", "520 AO-Sequenceable": "1", "555-Description": "DA device that is used as a shutter", "405-DA Device": "405 Shutter", "405 AO-TriggerSequenceLength": "1000", "640 AO-MinVolts": "0.0000", "640 Shutter-Block voltage": "false", "FrameIndex": 0, "PRIME-Port": "CMOS", "555 AO-MinVolts": "0.0000", "COM3-Description": "Serial port driver (boost:asio)", "528 AO-Description": "NI DAC", "555 AO-IOChannel": "Dev1/ao6", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "555 AO-TriggerInputLine": "/Dev1/PFI0", "PRIME-ActualInterval-ms": "374.3700", "LudlController-Port": "COM9", "445 Shutter-Demo": "No", "PRIME-Trigger-Expose Out-Mux": "1", "SliceIndex": 10, "PRIME-CCDTemperatureSetPoint": "-15.0000", "405 Shutter-MinVolts": "0.0000", "PRIME-ExposeOutMode": "First Row", "AxisPositions": null, "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Volts": "0.0000", "528-Name": "DA Shutter", "555-State": "0", "XLIGHT Dichroic-Label": "CFP/YFP", "445 AO-Sequenceable": "1", "405-Name": "DA Shutter", "405 AO-IOChannel": "Dev1/ao0", "Core-Camera": "PRIME", "Focus-Name": "Focus", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "405 Shutter-IOChannel": "Dev1/ao8", "640 AO-Demo": "No", "640 Shutter-SupportsTriggering": "0", "445 AO-Demo": "No", "555-DA Device": "555 Shutter", "470 Shutter-Description": "NI DAC", "528 AO-Volts": "0.0000", "COM1-DataBits": "8", "Vincent-D3-Address": "x", "520 AO-SupportsTriggering": "0", "405-Description": "DA device that is used as a shutter", "640 Shutter-MinVolts": "0.0000", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "405 AO-Name": "AnalogIO", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "640 AO-TriggerInputLine": "/Dev1/PFI0", "405 AO-TriggerInputLine": "/Dev1/PFI0", "640-Description": "DA device that is used as a shutter", "445 Shutter-TriggerSampleRate": "100000", "470 Shutter-TriggerSequenceLength": "1000", "COM1-StopBits": "1", "520 Shutter-SupportsTriggering": "0", "405-State": "0", "Core-Initialize": "1", "XLIGHT Emission Wheel-State": "7", "520 AO-Description": "NI DAC", "528 AO-TriggerInputLine": "/Dev1/PFI0", "445 Shutter-MaxVolts": "5.0000", "COM9-Handshaking": "Off", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "XLIGHT Spin motor-Label": "On (spinning)", "PRIME-PP 5 ENABLED": "No", "UUID": "da3da981-e1e4-430f-992f-4dbd43444c5d", "PRIME-TriggerMode": "Internal Trigger", "COM1-Handshaking": "Off", "PRIME-Timing-PostTriggerDelayNs": "49344000.0000", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "Focus-Position": "16479.8000", "528 Shutter-Block voltage": "false", "Channel": "Il-G", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "555 Shutter-Demo": "No", "405 Shutter-Demo": "No", "528 AO-Name": "AnalogIO", "520-State": "0", "Core-Galvo": "", "405 Shutter-TriggerSampleRate": "100000", "Focus-FocusStepSize_(nm)": "100", "PRIME-PixelType": "16bit", "COM4-DataBits": "8", "470 AO-IOChannel": "Dev1/ao2", "520 AO-MinVolts": "0.0000", "COM9-Parity": "None", "PRIME-CircularBufferEnabled": "ON", "PRIME-PP 6 Reset": "No", "640 AO-Description": "NI DAC", "Source": "PRIME", "445 AO-MinVolts": "0.0000", "PIEZO-Name": "Stage", "445 AO-Description": "NI DAC", "PRIME-CircularBufferFrameCount": "46", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "520 AO-Volts": "4.5000", "528 Shutter-TriggerSampleRate": "100000", "XPositionUm": 62321, "OpticalPath-State": "4", "PRIME-PP 3 THRESHOLD": "80", "PRIME-PVCAM Version": "3.7.1", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Gain": "1-HDR", "640 Shutter-TriggerSequenceLength": "1000", "528 AO-SupportsTriggering": "0", "445 AO-TriggerInputLine": "/Dev1/PFI0", "OpticalPath-Name": "OpticalPath", "PRIME-PP 4 ENABLED": "Yes", "PRIME-PVCAM Adapter Version": "1.3.29", "COM3-Name": "COM3", "405 AO-MaxVolts": "5.0000", "520 Shutter-Block voltage": "false", "PRIME-Timing-ClearingTimeNs": "49344000.0000", "Core-Focus": "PIEZO", "528 AO-Block voltage": "false", "TE2000-Description": "Nikon TE2000 hub", "528 AO-MinVolts": "0.0000", "COM4-Parity": "None", "Summary": { "Slices": 11, "ChContrastMin": [ 0, 0, 0, 0, 0, 0 ], "PixelAspect": 1, "SlicesFirst": true, "Interval_ms": 0, "Time": "2019-12-17 21:58:39 -0500", "Prefix": "seq_3", "InitialPositionList": [ { "Label": "Pos0", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.435], "Focus": [16480.2], "XYStage": [ 69822.40000000001, 20100.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos1", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.425], "Focus": [16480.3], "XYStage": [ 69004, 20101 ] }, "GridColumnIndex": 0 }, { "Label": "Pos2", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.44], "Focus": [16480.1], "XYStage": [ 63119.5, 20690.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos3", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.405], "Focus": [16480.2], "XYStage": [ 62321, 20690.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos4", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.415], "Focus": [16480], "XYStage": [ 56604.100000000006, 20688.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos5", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.42000000000002], "Focus": [16480.1], "XYStage": [ 56034.200000000004, 20688.800000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos6", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.455], "Focus": [16480], "XYStage": [ 50889.3, 20496.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos7", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.43], "Focus": [16480.2], "XYStage": [ 50101.600000000006, 20496.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos8", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.67000000000002], "Focus": [16479.9], "XYStage": [ 44818.600000000006, 20496.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos9", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.67000000000002], "Focus": [16480], "XYStage": [ 43897.9, 20436.800000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos10", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.675], "Focus": [16479.9], "XYStage": [ 39670.600000000006, 20664.9 ] }, "GridColumnIndex": 0 }, { "Label": "Pos11", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.69], "Focus": [16479.9], "XYStage": [ 38734, 20664.600000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos12", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.695], "Focus": [16480.1], "XYStage": [ 34037.200000000004, 20664.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos13", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.685], "Focus": [16480.1], "XYStage": [ 33152, 20664.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos14", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.69], "Focus": [16480.1], "XYStage": [ 28122.800000000003, 20664.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos15", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.675], "Focus": [16480], "XYStage": [ 27422.9, 20664.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos16", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.64000000000001], "Focus": [16480], "XYStage": [ 23005.2, 20664.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos17", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.69], "Focus": [16479.9], "XYStage": [ 21820.600000000002, 20409.600000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos18", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.41], "Focus": [16480.1], "XYStage": [ 73697.6, -13222.800000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos19", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.455], "Focus": [16480.1], "XYStage": [ 72765.8, -13223 ] }, "GridColumnIndex": 0 }, { "Label": "Pos20", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.4], "Focus": [16480.2], "XYStage": [ 67445.5, -12303.800000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos21", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.415], "Focus": [16480.2], "XYStage": [ 66613.3, -12303.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos22", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.415], "Focus": [16480.1], "XYStage": [ 61819.4, -11936.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos23", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.405], "Focus": [16480.2], "XYStage": [ 61325.700000000004, -11937.1 ] }, "GridColumnIndex": 0 }, { "Label": "Pos24", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.46], "Focus": [16480.2], "XYStage": [ 55377, -10974.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos25", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.445], "Focus": [16480.1], "XYStage": [ 54827, -11214.800000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos26", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.435], "Focus": [16480.2], "XYStage": [ 49937.100000000006, -10919.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos27", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.425], "Focus": [16480.1], "XYStage": [ 48764, -10919.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos28", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.42000000000002], "Focus": [16480.2], "XYStage": [ 43978.4, -10920.400000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos29", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.435], "Focus": [16480.2], "XYStage": [ 43106.3, -10920.400000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos30", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.405], "Focus": [16480.3], "XYStage": [ 38212.4, -10920.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos31", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.445], "Focus": [16480.2], "XYStage": [ 37254.9, -10920.400000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos32", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.44], "Focus": [16480.3], "XYStage": [ 32472.800000000003, -11294.400000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos33", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [166.95000000000002], "Focus": [16480.2], "XYStage": [ 31420.7, -11294.400000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos34", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [166.95000000000002], "Focus": [16480.2], "XYStage": [ 26881.5, -11640 ] }, "GridColumnIndex": 0 }, { "Label": "Pos35", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [166.98], "Focus": [16480.2], "XYStage": [ 25876.100000000002, -11641.1 ] }, "GridColumnIndex": 0 } ], "z-step_um": 10, "GridRow": 0, "BitDepth": 16, "Depth": 2, "Channels": 6, "Source": "Micro-Manager", "ChColors": [ -16711681, -256, -65281, -1, -1, -16776961 ], "MetadataVersion": 10, "KeepShutterOpenChannels": true, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536, 65536 ], "CustomIntervals_ms": [], "Height": 1200, "MicroManagerVersion": "1.4.23 20180219", "GridColumn": 0, "UUID": "8c8f9d20-b128-4577-9f91-89e70f22d319", "PixelType": "GRAY16", "Width": 1200, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\Yan\\20191217-YC113-S5S6", "Frames": 1, "Comment": "", "UserName": "Nikon", "PixelSize_um": 1.1, "ROI": [ 0, 0, 1200, 1200 ], "Date": "2019-12-17", "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC", "P-DAPI-high" ], "KeepShutterOpenSlices": true, "IJType": 1, "ComputerName": "ZadorConfocal2", "Positions": 36, "PositionIndex": 3, "CameraTimeout": 20000, "TimeFirst": false }, "640 AO-Name": "AnalogIO", "Vincent-D3-Command": "Close" }, "FrameKey-0-1-0": { "COM4-StopBits": "1", "528 AO-MaxVolts": "5.0000", "XLIGHT Excitation Wheel-State": "2", "470 Shutter-Name": "AnalogIO", "Core-Shutter": "555", "555 AO-Demo": "No", "XLIGHT Dichroic-State": "2", "XYStage-StepSize-Y": "0.1000", "ZPositionUm": 127.405, "XYStage-StepSize-X": "0.1000", "PRIME-FirmwareVersion": "20.28", "PRIME-ClearCycles": "2", "520-Name": "DA Shutter", "405 Shutter-Name": "AnalogIO", "PIEZO-StepSize": "0.0050", "PRIME-ClearMode": "Pre-Exposure", "470 AO-MinVolts": "0.0000", "405 AO-SupportsTriggering": "1", "CameraChannelIndex": 0, "XYStage-StartSpeed": "500.0000", "COM9-StopBits": "2", "405 AO-Description": "NI DAC", "COM4-DelayBetweenCharsMs": "0.0000", "445 AO-Block voltage": "false", "520 AO-TriggerInputLine": "/Dev1/PFI0", "555 AO-Description": "NI DAC", "520 AO-TriggerSampleRate": "100000", "COM4-Verbose": "1", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM4-Name": "COM4", "405 Shutter-Volts": "0.0000", "Vincent-D3-Port": "COM1", "PRIME-PP 4 MIN ADU AFFECTED": "200", "470 Shutter-MinVolts": "0.0000", "PRIME-PP 3 ENABLED": "Yes", "470 AO-Description": "NI DAC", "PRIME-Description": "PVCAM API device adapter", "520 Shutter-MaxVolts": "5.0000", "520 Shutter-Volts": "0.0000", "528 AO-Sequenceable": "1", "PRIME-Binning": "1x1", "555 Shutter-MaxVolts": "5.0000", "528 Shutter-TriggerSequenceLength": "1000", "405 Shutter-Block voltage": "false", "XLIGHT Spin motor-State": "1", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "Vincent-D3-Time to open (ms)": "35.0000", "Vincent-D3-Time to close (ms)": "35.0000", "445 AO-TriggerSampleRate": "100000", "640 Shutter-IOChannel": "Dev1/ao14", "555 AO-Sequenceable": "1", "520 AO-Name": "AnalogIO", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "470 AO-Block voltage": "false", "COM1-Verbose": "1", "COM9-AnswerTimeout": "10000.0000", "528 Shutter-Name": "AnalogIO", "PRIME-Trigger Timeout (secs)": "10", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "NextFrame": 0, "470 AO-TriggerSequenceLength": "1000", "PRIME-TransposeXY": "0", "445 AO-TriggerSequenceLength": "1000", "COM1-Name": "COM1", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "COM9-DataBits": "8", "COM1-AnswerTimeout": "500.0000", "Camera": "", "TE2000-Version": "V2.50-V2.50-V2.10", "528 Shutter-Volts": "0.0000", "XLIGHT Hub-Name": "XLIGHT Hub", "PIEZO-Description": "Ludl stage driver adapter", "PositionName": "Pos3", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSampleRate": "100000", "PRIME-FanSpeedSetpoint": "High", "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "405 AO-TriggerSampleRate": "100000", "470 Shutter-Block voltage": "false", "470-State": "0", "COM3-Parity": "None", "405 AO-Volts": "0.0000", "PRIME-TransposeMirrorY": "0", "PRIME-ChipName": "GS144BSI", "470 AO-Volts": "0.0000", "PRIME-TransposeMirrorX": "0", "445 Shutter-SupportsTriggering": "0", "470 Shutter-SupportsTriggering": "0", "XLIGHT Hub-Description": "XLIGHT hub", "PRIME-PreampOffLimit": "10000", "470 AO-Sequenceable": "1", "528 Shutter-MaxVolts": "5.0000", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "Core-XYStage": "XYStage", "470 AO-TriggerSampleRate": "100000", "PRIME-Timing-PreTriggerDelayNs": "24672000.0000", "528-DA Device": "528 Shutter", "Focus-Description": "Nikon TE2000 focus adapter", "FilterBlock-State": "5", "520 AO-Demo": "No", "COM3-DelayBetweenCharsMs": "0.0000", "470 Shutter-Volts": "0.0000", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "PRIME-PP 2 THRESHOLD": "125", "PRIME-PP 4 THRESHOLD": "75", "XYStage-Acceleration": "4.0000", "470 Shutter-Demo": "No", "PRIME-Name": "PMPCIECam00", "640-DA Device": "640 Shutter", "405 AO-Block voltage": "false", "ElapsedTime-ms": 139261, "PRIME-Offset": "100", "445 AO-IOChannel": "Dev1/ao1", "640 AO-MaxVolts": "5.0000", "PositionIndex": 3, "PRIME-PP 1 ENABLED": "Yes", "COM1-BaudRate": "9600", "640-State": "0", "COM3-Verbose": "1", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "LudlController-TransmissionDelay": "0", "PRIME-FullWellCapacity": "62000", "PRIME-SMARTStreamingEnabled": "No", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "640 Shutter-Description": "NI DAC", "528-Description": "DA device that is used as a shutter", "COM4-Description": "Serial port driver (boost:asio)", "405 Shutter-Description": "NI DAC", "TE2000-Name": "TE2000", "528 Shutter-Description": "NI DAC", "COM9-Description": "Serial port driver (boost:asio)", "LudlController-Name": "LudlController", "445 Shutter-TriggerSequenceLength": "1000", "PRIME-SerialNumber": "A18B203008", "445 AO-Volts": "0.0000", "PRIME-X-dimension": "1200", "PRIME-ShutterCloseDelay": "0", "XLIGHT Disk slider-State": "2", "Position": "Default", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 AO-IOChannel": "Dev1/ao5", "520 AO-IOChannel": "Dev1/ao4", "528 AO-TriggerSequenceLength": "1000", "405 Shutter-MaxVolts": "5.0000", "470-DA Device": "470 Shutter", "PRIME-FTCapable": "No", "Core-ChannelGroup": "Channels", "555 AO-SupportsTriggering": "0", "PRIME-Y-dimension": "1200", "Frame": 0, "555 Shutter-Sequenceable": "1", "PRIME-Timing-ExposureTimeNs": "9992000.0000", "Core-ImageProcessor": "", "528-State": "0", "520 AO-Block voltage": "false", "XLIGHT Excitation Wheel-Label": "QUAD", "640 Shutter-Demo": "No", "COM3-StopBits": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "520 Shutter-IOChannel": "Dev1/ao11", "Width": 1200, "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-SupportsTriggering": "0", "640 AO-TriggerSampleRate": "100000", "520-Description": "DA device that is used as a shutter", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "405 Shutter-Sequenceable": "1", "405 Shutter-SupportsTriggering": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-TriggerSampleRate": "100000", "640 Shutter-MaxVolts": "5.0000", "555 AO-MaxVolts": "5.0000", "520 AO-TriggerSequenceLength": "1000", "COM3-BaudRate": "9600", "COM9-BaudRate": "9600", "Vincent-D3-Channel #": "Ch. #1", "PRIME-PMode": "Normal", "OpticalPath-Label": "Left(100)", "BitDepth": 16, "555 AO-TriggerSequenceLength": "1000", "445 Shutter-Description": "NI DAC", "405 AO-Sequenceable": "1", "PRIME-AcquisitionMethod": "Callbacks", "555 AO-Name": "AnalogIO", "PRIME-ShutterMode": "Pre-Exposure", "COM3-Handshaking": "Off", "445 AO-SupportsTriggering": "0", "ROI": "0-0-1200-1200", "COM9-Verbose": "1", "PRIME-Color": "OFF", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "PRIME-ShutterOpenDelay": "0", "COM1-Parity": "None", "Slice": 0, "SlicePosition": -50, "COM9-DelayBetweenCharsMs": "0.0000", "555-Name": "DA Shutter", "PIEZO-LudlSingleAxisName": "B", "XLIGHT Emission Wheel-Label": "585/11(T)", "640 Shutter-Name": "AnalogIO", "640 AO-Sequenceable": "1", "520 Shutter-Demo": "No", "528 Shutter-Demo": "No", "470-Name": "DA Shutter", "520-DA Device": "520 Shutter", "XYStage-Description": "Ludl XY stage driver adapter", "445 Shutter-MinVolts": "0.0000", "528 AO-TriggerSampleRate": "100000", "405 AO-MinVolts": "0.0000", "405 Shutter-TriggerSequenceLength": "1000", "Height": 1200, "555 AO-Block voltage": "false", "640 Shutter-Volts": "0.0000", "PixelType": "GRAY16", "445 Shutter-Block voltage": "false", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "520 Shutter-TriggerSequenceLength": "1000", "COM9-Name": "COM9", "Core-SLM": "", "555 AO-Volts": "4.5000", "555 Shutter-Block voltage": "false", "XYStage-Speed": "1800.0000", "520 AO-MaxVolts": "5.0000", "528 Shutter-SupportsTriggering": "0", "COM1-Description": "Serial port driver (boost:asio)", "PRIME-MetadataEnabled": "No", "520 Shutter-MinVolts": "0.0000", "470 Shutter-Sequenceable": "1", "PRIME-PreampDelay": "0", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-MinVolts": "0.0000", "ChannelIndex": 1, "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Core-AutoShutter": "1", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "Exposure-ms": 160, "PRIME-PP 2 ENABLED": "Yes", "640 Shutter-TriggerSampleRate": "100000", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "470 Shutter-IOChannel": "Dev1/ao10", "405 AO-Demo": "No", "PixelSizeUm": 1.1, "470 AO-Demo": "No", "470 Shutter-MaxVolts": "5.0000", "Vincent-D3-Name": "Vincent-D3", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FileName": "img_000000000_Il-T_000.tif", "LudlController-Reset": "Operate", "PRIME-Exposure": "160.00", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "445-Name": "DA Shutter", "555 Shutter-Name": "AnalogIO", "520 Shutter-TriggerSampleRate": "100000", "445 Shutter-IOChannel": "Dev1/ao9", "PRIME-TransposeCorrection": "0", "XYStage-StepSize": "0.1000", "445-Description": "DA device that is used as a shutter", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 AO-IOChannel": "Dev1/ao7", "520 Shutter-Name": "AnalogIO", "COM3-DataBits": "8", "640 AO-Volts": "0.0000", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "555 Shutter-IOChannel": "Dev1/ao13", "WaitInterval": null, "520 Shutter-Sequenceable": "1", "YPositionUm": 20690.300000000003, "445-State": "0", "445-DA Device": "445 Shutter", "PRIME-CircularBufferAutoSize": "ON", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "555 AO-TriggerSampleRate": "100000", "445 Shutter-Volts": "0.0000", "XLIGHT Hub-Port": "COM3", "Core-AutoFocus": "", "COM4-Handshaking": "Off", "528 Shutter-MinVolts": "0.0000", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "445 Shutter-Name": "AnalogIO", "528 Shutter-Sequenceable": "1", "XYStage-TransposeMirrorX": "0", "XYStage-TransposeMirrorY": "0", "PRIME-ReadoutRate": "100MHz 16bit", "XYStage-Name": "Stage", "640 AO-SupportsTriggering": "0", "640-Name": "DA Shutter", "640 Shutter-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "640 AO-Block voltage": "false", "445 Shutter-Sequenceable": "1", "470 AO-MaxVolts": "5.0000", "COM4-BaudRate": "9600", "470-Description": "DA device that is used as a shutter", "520 Shutter-Description": "NI DAC", "PRIME-CCDTemperature": "-14.9600", "Time": "2019-12-17 21:58:46 -0500", "640 AO-TriggerSequenceLength": "1000", "470 AO-Name": "AnalogIO", "XLIGHT Disk slider-Label": "Disk Pos 40um", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "Binning": "1x1", "TE2000-ModelType": "1", "COM3-AnswerTimeout": "500.0000", "555 Shutter-SupportsTriggering": "0", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "TE2000-Port": "COM4", "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Description": "NI DAC", "COM4-AnswerTimeout": "500.0000", "COM1-DelayBetweenCharsMs": "0.0000", "FilterBlock-Label": "Confocal", "445 AO-Name": "AnalogIO", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-Timing-ReadoutTimeNs": "24672000.0000", "528 AO-Demo": "No", "520 AO-Sequenceable": "1", "555-Description": "DA device that is used as a shutter", "405-DA Device": "405 Shutter", "405 AO-TriggerSequenceLength": "1000", "640 AO-MinVolts": "0.0000", "640 Shutter-Block voltage": "false", "FrameIndex": 0, "PRIME-Port": "CMOS", "555 AO-MinVolts": "0.0000", "COM3-Description": "Serial port driver (boost:asio)", "528 AO-Description": "NI DAC", "555 AO-IOChannel": "Dev1/ao6", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "555 AO-TriggerInputLine": "/Dev1/PFI0", "PRIME-ActualInterval-ms": "374.3700", "LudlController-Port": "COM9", "445 Shutter-Demo": "No", "PRIME-Trigger-Expose Out-Mux": "1", "SliceIndex": 0, "PRIME-CCDTemperatureSetPoint": "-15.0000", "405 Shutter-MinVolts": "0.0000", "PRIME-ExposeOutMode": "First Row", "AxisPositions": null, "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Volts": "5.0000", "528-Name": "DA Shutter", "555-State": "1", "XLIGHT Dichroic-Label": "QUAD", "445 AO-Sequenceable": "1", "405-Name": "DA Shutter", "405 AO-IOChannel": "Dev1/ao0", "Core-Camera": "PRIME", "Focus-Name": "Focus", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "405 Shutter-IOChannel": "Dev1/ao8", "640 AO-Demo": "No", "640 Shutter-SupportsTriggering": "0", "445 AO-Demo": "No", "555-DA Device": "555 Shutter", "470 Shutter-Description": "NI DAC", "528 AO-Volts": "0.0000", "COM1-DataBits": "8", "Vincent-D3-Address": "x", "520 AO-SupportsTriggering": "0", "405-Description": "DA device that is used as a shutter", "640 Shutter-MinVolts": "0.0000", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "405 AO-Name": "AnalogIO", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "640 AO-TriggerInputLine": "/Dev1/PFI0", "405 AO-TriggerInputLine": "/Dev1/PFI0", "640-Description": "DA device that is used as a shutter", "445 Shutter-TriggerSampleRate": "100000", "470 Shutter-TriggerSequenceLength": "1000", "COM1-StopBits": "1", "520 Shutter-SupportsTriggering": "0", "405-State": "0", "Core-Initialize": "1", "XLIGHT Emission Wheel-State": "7", "520 AO-Description": "NI DAC", "528 AO-TriggerInputLine": "/Dev1/PFI0", "445 Shutter-MaxVolts": "5.0000", "COM9-Handshaking": "Off", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "XLIGHT Spin motor-Label": "On (spinning)", "PRIME-PP 5 ENABLED": "No", "UUID": "3ae4911c-08fc-4c8e-9163-54989e013859", "PRIME-TriggerMode": "Internal Trigger", "COM1-Handshaking": "Off", "PRIME-Timing-PostTriggerDelayNs": "49344000.0000", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "Focus-Position": "16479.8000", "528 Shutter-Block voltage": "false", "Channel": "Il-T", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "555 Shutter-Demo": "No", "405 Shutter-Demo": "No", "528 AO-Name": "AnalogIO", "520-State": "0", "Core-Galvo": "", "405 Shutter-TriggerSampleRate": "100000", "Focus-FocusStepSize_(nm)": "100", "PRIME-PixelType": "16bit", "COM4-DataBits": "8", "470 AO-IOChannel": "Dev1/ao2", "520 AO-MinVolts": "0.0000", "COM9-Parity": "None", "PRIME-CircularBufferEnabled": "ON", "PRIME-PP 6 Reset": "No", "640 AO-Description": "NI DAC", "Source": "PRIME", "445 AO-MinVolts": "0.0000", "PIEZO-Name": "Stage", "445 AO-Description": "NI DAC", "PRIME-CircularBufferFrameCount": "46", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "520 AO-Volts": "0.0000", "528 Shutter-TriggerSampleRate": "100000", "XPositionUm": 62321, "OpticalPath-State": "4", "PRIME-PP 3 THRESHOLD": "80", "PRIME-PVCAM Version": "3.7.1", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Gain": "1-HDR", "640 Shutter-TriggerSequenceLength": "1000", "528 AO-SupportsTriggering": "0", "445 AO-TriggerInputLine": "/Dev1/PFI0", "OpticalPath-Name": "OpticalPath", "PRIME-PP 4 ENABLED": "Yes", "PRIME-PVCAM Adapter Version": "1.3.29", "COM3-Name": "COM3", "405 AO-MaxVolts": "5.0000", "520 Shutter-Block voltage": "false", "PRIME-Timing-ClearingTimeNs": "49344000.0000", "Core-Focus": "PIEZO", "528 AO-Block voltage": "false", "TE2000-Description": "Nikon TE2000 hub", "528 AO-MinVolts": "0.0000", "COM4-Parity": "None", "Summary": { "Slices": 11, "ChContrastMin": [ 0, 0, 0, 0, 0, 0 ], "PixelAspect": 1, "SlicesFirst": true, "Interval_ms": 0, "Time": "2019-12-17 21:58:39 -0500", "Prefix": "seq_3", "InitialPositionList": [ { "Label": "Pos0", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.435], "Focus": [16480.2], "XYStage": [ 69822.40000000001, 20100.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos1", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.425], "Focus": [16480.3], "XYStage": [ 69004, 20101 ] }, "GridColumnIndex": 0 }, { "Label": "Pos2", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.44], "Focus": [16480.1], "XYStage": [ 63119.5, 20690.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos3", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.405], "Focus": [16480.2], "XYStage": [ 62321, 20690.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos4", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.415], "Focus": [16480], "XYStage": [ 56604.100000000006, 20688.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos5", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.42000000000002], "Focus": [16480.1], "XYStage": [ 56034.200000000004, 20688.800000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos6", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.455], "Focus": [16480], "XYStage": [ 50889.3, 20496.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos7", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.43], "Focus": [16480.2], "XYStage": [ 50101.600000000006, 20496.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos8", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.67000000000002], "Focus": [16479.9], "XYStage": [ 44818.600000000006, 20496.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos9", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.67000000000002], "Focus": [16480], "XYStage": [ 43897.9, 20436.800000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos10", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.675], "Focus": [16479.9], "XYStage": [ 39670.600000000006, 20664.9 ] }, "GridColumnIndex": 0 }, { "Label": "Pos11", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.69], "Focus": [16479.9], "XYStage": [ 38734, 20664.600000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos12", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.695], "Focus": [16480.1], "XYStage": [ 34037.200000000004, 20664.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos13", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.685], "Focus": [16480.1], "XYStage": [ 33152, 20664.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos14", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.69], "Focus": [16480.1], "XYStage": [ 28122.800000000003, 20664.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos15", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.675], "Focus": [16480], "XYStage": [ 27422.9, 20664.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos16", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.64000000000001], "Focus": [16480], "XYStage": [ 23005.2, 20664.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos17", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.69], "Focus": [16479.9], "XYStage": [ 21820.600000000002, 20409.600000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos18", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.41], "Focus": [16480.1], "XYStage": [ 73697.6, -13222.800000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos19", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.455], "Focus": [16480.1], "XYStage": [ 72765.8, -13223 ] }, "GridColumnIndex": 0 }, { "Label": "Pos20", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.4], "Focus": [16480.2], "XYStage": [ 67445.5, -12303.800000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos21", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.415], "Focus": [16480.2], "XYStage": [ 66613.3, -12303.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos22", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.415], "Focus": [16480.1], "XYStage": [ 61819.4, -11936.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos23", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.405], "Focus": [16480.2], "XYStage": [ 61325.700000000004, -11937.1 ] }, "GridColumnIndex": 0 }, { "Label": "Pos24", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.46], "Focus": [16480.2], "XYStage": [ 55377, -10974.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos25", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.445], "Focus": [16480.1], "XYStage": [ 54827, -11214.800000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos26", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.435], "Focus": [16480.2], "XYStage": [ 49937.100000000006, -10919.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos27", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.425], "Focus": [16480.1], "XYStage": [ 48764, -10919.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos28", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.42000000000002], "Focus": [16480.2], "XYStage": [ 43978.4, -10920.400000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos29", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.435], "Focus": [16480.2], "XYStage": [ 43106.3, -10920.400000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos30", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.405], "Focus": [16480.3], "XYStage": [ 38212.4, -10920.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos31", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.445], "Focus": [16480.2], "XYStage": [ 37254.9, -10920.400000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos32", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.44], "Focus": [16480.3], "XYStage": [ 32472.800000000003, -11294.400000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos33", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [166.95000000000002], "Focus": [16480.2], "XYStage": [ 31420.7, -11294.400000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos34", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [166.95000000000002], "Focus": [16480.2], "XYStage": [ 26881.5, -11640 ] }, "GridColumnIndex": 0 }, { "Label": "Pos35", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [166.98], "Focus": [16480.2], "XYStage": [ 25876.100000000002, -11641.1 ] }, "GridColumnIndex": 0 } ], "z-step_um": 10, "GridRow": 0, "BitDepth": 16, "Depth": 2, "Channels": 6, "Source": "Micro-Manager", "ChColors": [ -16711681, -256, -65281, -1, -1, -16776961 ], "MetadataVersion": 10, "KeepShutterOpenChannels": true, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536, 65536 ], "CustomIntervals_ms": [], "Height": 1200, "MicroManagerVersion": "1.4.23 20180219", "GridColumn": 0, "UUID": "8c8f9d20-b128-4577-9f91-89e70f22d319", "PixelType": "GRAY16", "Width": 1200, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\Yan\\20191217-YC113-S5S6", "Frames": 1, "Comment": "", "UserName": "Nikon", "PixelSize_um": 1.1, "ROI": [ 0, 0, 1200, 1200 ], "Date": "2019-12-17", "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC", "P-DAPI-high" ], "KeepShutterOpenSlices": true, "IJType": 1, "ComputerName": "ZadorConfocal2", "Positions": 36, "PositionIndex": 3, "CameraTimeout": 20000, "TimeFirst": false }, "640 AO-Name": "AnalogIO", "Vincent-D3-Command": "Close" }, "FrameKey-0-1-1": { "COM4-StopBits": "1", "528 AO-MaxVolts": "5.0000", "XLIGHT Excitation Wheel-State": "2", "470 Shutter-Name": "AnalogIO", "Core-Shutter": "555", "555 AO-Demo": "No", "XLIGHT Dichroic-State": "2", "XYStage-StepSize-Y": "0.1000", "ZPositionUm": 137.405, "XYStage-StepSize-X": "0.1000", "PRIME-FirmwareVersion": "20.28", "PRIME-ClearCycles": "2", "520-Name": "DA Shutter", "405 Shutter-Name": "AnalogIO", "PIEZO-StepSize": "0.0050", "PRIME-ClearMode": "Pre-Exposure", "470 AO-MinVolts": "0.0000", "405 AO-SupportsTriggering": "1", "CameraChannelIndex": 0, "XYStage-StartSpeed": "500.0000", "COM9-StopBits": "2", "405 AO-Description": "NI DAC", "COM4-DelayBetweenCharsMs": "0.0000", "445 AO-Block voltage": "false", "520 AO-TriggerInputLine": "/Dev1/PFI0", "555 AO-Description": "NI DAC", "520 AO-TriggerSampleRate": "100000", "COM4-Verbose": "1", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM4-Name": "COM4", "405 Shutter-Volts": "0.0000", "Vincent-D3-Port": "COM1", "PRIME-PP 4 MIN ADU AFFECTED": "200", "470 Shutter-MinVolts": "0.0000", "PRIME-PP 3 ENABLED": "Yes", "470 AO-Description": "NI DAC", "PRIME-Description": "PVCAM API device adapter", "520 Shutter-MaxVolts": "5.0000", "520 Shutter-Volts": "0.0000", "528 AO-Sequenceable": "1", "PRIME-Binning": "1x1", "555 Shutter-MaxVolts": "5.0000", "528 Shutter-TriggerSequenceLength": "1000", "405 Shutter-Block voltage": "false", "XLIGHT Spin motor-State": "1", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "Vincent-D3-Time to open (ms)": "35.0000", "Vincent-D3-Time to close (ms)": "35.0000", "445 AO-TriggerSampleRate": "100000", "640 Shutter-IOChannel": "Dev1/ao14", "555 AO-Sequenceable": "1", "520 AO-Name": "AnalogIO", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "470 AO-Block voltage": "false", "COM1-Verbose": "1", "COM9-AnswerTimeout": "10000.0000", "528 Shutter-Name": "AnalogIO", "PRIME-Trigger Timeout (secs)": "10", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "NextFrame": 0, "470 AO-TriggerSequenceLength": "1000", "PRIME-TransposeXY": "0", "445 AO-TriggerSequenceLength": "1000", "COM1-Name": "COM1", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "COM9-DataBits": "8", "COM1-AnswerTimeout": "500.0000", "Camera": "", "TE2000-Version": "V2.50-V2.50-V2.10", "528 Shutter-Volts": "0.0000", "XLIGHT Hub-Name": "XLIGHT Hub", "PIEZO-Description": "Ludl stage driver adapter", "PositionName": "Pos3", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSampleRate": "100000", "PRIME-FanSpeedSetpoint": "High", "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "405 AO-TriggerSampleRate": "100000", "470 Shutter-Block voltage": "false", "470-State": "0", "COM3-Parity": "None", "405 AO-Volts": "0.0000", "PRIME-TransposeMirrorY": "0", "PRIME-ChipName": "GS144BSI", "470 AO-Volts": "0.0000", "PRIME-TransposeMirrorX": "0", "445 Shutter-SupportsTriggering": "0", "470 Shutter-SupportsTriggering": "0", "XLIGHT Hub-Description": "XLIGHT hub", "PRIME-PreampOffLimit": "10000", "470 AO-Sequenceable": "1", "528 Shutter-MaxVolts": "5.0000", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "Core-XYStage": "XYStage", "470 AO-TriggerSampleRate": "100000", "PRIME-Timing-PreTriggerDelayNs": "24672000.0000", "528-DA Device": "528 Shutter", "Focus-Description": "Nikon TE2000 focus adapter", "FilterBlock-State": "5", "520 AO-Demo": "No", "COM3-DelayBetweenCharsMs": "0.0000", "470 Shutter-Volts": "0.0000", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "PRIME-PP 2 THRESHOLD": "125", "PRIME-PP 4 THRESHOLD": "75", "XYStage-Acceleration": "4.0000", "470 Shutter-Demo": "No", "PRIME-Name": "PMPCIECam00", "640-DA Device": "640 Shutter", "405 AO-Block voltage": "false", "ElapsedTime-ms": 139706, "PRIME-Offset": "100", "445 AO-IOChannel": "Dev1/ao1", "640 AO-MaxVolts": "5.0000", "PositionIndex": 3, "PRIME-PP 1 ENABLED": "Yes", "COM1-BaudRate": "9600", "640-State": "0", "COM3-Verbose": "1", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "LudlController-TransmissionDelay": "0", "PRIME-FullWellCapacity": "62000", "PRIME-SMARTStreamingEnabled": "No", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "640 Shutter-Description": "NI DAC", "528-Description": "DA device that is used as a shutter", "COM4-Description": "Serial port driver (boost:asio)", "405 Shutter-Description": "NI DAC", "TE2000-Name": "TE2000", "528 Shutter-Description": "NI DAC", "COM9-Description": "Serial port driver (boost:asio)", "LudlController-Name": "LudlController", "445 Shutter-TriggerSequenceLength": "1000", "PRIME-SerialNumber": "A18B203008", "445 AO-Volts": "0.0000", "PRIME-X-dimension": "1200", "PRIME-ShutterCloseDelay": "0", "XLIGHT Disk slider-State": "2", "Position": "Default", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 AO-IOChannel": "Dev1/ao5", "520 AO-IOChannel": "Dev1/ao4", "528 AO-TriggerSequenceLength": "1000", "405 Shutter-MaxVolts": "5.0000", "470-DA Device": "470 Shutter", "PRIME-FTCapable": "No", "Core-ChannelGroup": "Channels", "555 AO-SupportsTriggering": "0", "PRIME-Y-dimension": "1200", "Frame": 0, "555 Shutter-Sequenceable": "1", "PRIME-Timing-ExposureTimeNs": "9992000.0000", "Core-ImageProcessor": "", "528-State": "0", "520 AO-Block voltage": "false", "XLIGHT Excitation Wheel-Label": "QUAD", "640 Shutter-Demo": "No", "COM3-StopBits": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "520 Shutter-IOChannel": "Dev1/ao11", "Width": 1200, "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-SupportsTriggering": "0", "640 AO-TriggerSampleRate": "100000", "520-Description": "DA device that is used as a shutter", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "405 Shutter-Sequenceable": "1", "405 Shutter-SupportsTriggering": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-TriggerSampleRate": "100000", "640 Shutter-MaxVolts": "5.0000", "555 AO-MaxVolts": "5.0000", "520 AO-TriggerSequenceLength": "1000", "COM3-BaudRate": "9600", "COM9-BaudRate": "9600", "Vincent-D3-Channel #": "Ch. #1", "PRIME-PMode": "Normal", "OpticalPath-Label": "Left(100)", "BitDepth": 16, "555 AO-TriggerSequenceLength": "1000", "445 Shutter-Description": "NI DAC", "405 AO-Sequenceable": "1", "PRIME-AcquisitionMethod": "Callbacks", "555 AO-Name": "AnalogIO", "PRIME-ShutterMode": "Pre-Exposure", "COM3-Handshaking": "Off", "445 AO-SupportsTriggering": "0", "ROI": "0-0-1200-1200", "COM9-Verbose": "1", "PRIME-Color": "OFF", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "PRIME-ShutterOpenDelay": "0", "COM1-Parity": "None", "Slice": 1, "SlicePosition": -40, "COM9-DelayBetweenCharsMs": "0.0000", "555-Name": "DA Shutter", "PIEZO-LudlSingleAxisName": "B", "XLIGHT Emission Wheel-Label": "585/11(T)", "640 Shutter-Name": "AnalogIO", "640 AO-Sequenceable": "1", "520 Shutter-Demo": "No", "528 Shutter-Demo": "No", "470-Name": "DA Shutter", "520-DA Device": "520 Shutter", "XYStage-Description": "Ludl XY stage driver adapter", "445 Shutter-MinVolts": "0.0000", "528 AO-TriggerSampleRate": "100000", "405 AO-MinVolts": "0.0000", "405 Shutter-TriggerSequenceLength": "1000", "Height": 1200, "555 AO-Block voltage": "false", "640 Shutter-Volts": "0.0000", "PixelType": "GRAY16", "445 Shutter-Block voltage": "false", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "520 Shutter-TriggerSequenceLength": "1000", "COM9-Name": "COM9", "Core-SLM": "", "555 AO-Volts": "4.5000", "555 Shutter-Block voltage": "false", "XYStage-Speed": "1800.0000", "520 AO-MaxVolts": "5.0000", "528 Shutter-SupportsTriggering": "0", "COM1-Description": "Serial port driver (boost:asio)", "PRIME-MetadataEnabled": "No", "520 Shutter-MinVolts": "0.0000", "470 Shutter-Sequenceable": "1", "PRIME-PreampDelay": "0", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-MinVolts": "0.0000", "ChannelIndex": 1, "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Core-AutoShutter": "1", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "Exposure-ms": 160, "PRIME-PP 2 ENABLED": "Yes", "640 Shutter-TriggerSampleRate": "100000", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "470 Shutter-IOChannel": "Dev1/ao10", "405 AO-Demo": "No", "PixelSizeUm": 1.1, "470 AO-Demo": "No", "470 Shutter-MaxVolts": "5.0000", "Vincent-D3-Name": "Vincent-D3", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FileName": "img_000000000_Il-T_001.tif", "LudlController-Reset": "Operate", "PRIME-Exposure": "160.00", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "445-Name": "DA Shutter", "555 Shutter-Name": "AnalogIO", "520 Shutter-TriggerSampleRate": "100000", "445 Shutter-IOChannel": "Dev1/ao9", "PRIME-TransposeCorrection": "0", "XYStage-StepSize": "0.1000", "445-Description": "DA device that is used as a shutter", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 AO-IOChannel": "Dev1/ao7", "520 Shutter-Name": "AnalogIO", "COM3-DataBits": "8", "640 AO-Volts": "0.0000", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "555 Shutter-IOChannel": "Dev1/ao13", "WaitInterval": null, "520 Shutter-Sequenceable": "1", "YPositionUm": 20690.300000000003, "445-State": "0", "445-DA Device": "445 Shutter", "PRIME-CircularBufferAutoSize": "ON", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "555 AO-TriggerSampleRate": "100000", "445 Shutter-Volts": "0.0000", "XLIGHT Hub-Port": "COM3", "Core-AutoFocus": "", "COM4-Handshaking": "Off", "528 Shutter-MinVolts": "0.0000", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "445 Shutter-Name": "AnalogIO", "528 Shutter-Sequenceable": "1", "XYStage-TransposeMirrorX": "0", "XYStage-TransposeMirrorY": "0", "PRIME-ReadoutRate": "100MHz 16bit", "XYStage-Name": "Stage", "640 AO-SupportsTriggering": "0", "640-Name": "DA Shutter", "640 Shutter-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "640 AO-Block voltage": "false", "445 Shutter-Sequenceable": "1", "470 AO-MaxVolts": "5.0000", "COM4-BaudRate": "9600", "470-Description": "DA device that is used as a shutter", "520 Shutter-Description": "NI DAC", "PRIME-CCDTemperature": "-14.9600", "Time": "2019-12-17 21:58:47 -0500", "640 AO-TriggerSequenceLength": "1000", "470 AO-Name": "AnalogIO", "XLIGHT Disk slider-Label": "Disk Pos 40um", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "Binning": "1x1", "TE2000-ModelType": "1", "COM3-AnswerTimeout": "500.0000", "555 Shutter-SupportsTriggering": "0", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "TE2000-Port": "COM4", "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Description": "NI DAC", "COM4-AnswerTimeout": "500.0000", "COM1-DelayBetweenCharsMs": "0.0000", "FilterBlock-Label": "Confocal", "445 AO-Name": "AnalogIO", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-Timing-ReadoutTimeNs": "24672000.0000", "528 AO-Demo": "No", "520 AO-Sequenceable": "1", "555-Description": "DA device that is used as a shutter", "405-DA Device": "405 Shutter", "405 AO-TriggerSequenceLength": "1000", "640 AO-MinVolts": "0.0000", "640 Shutter-Block voltage": "false", "FrameIndex": 0, "PRIME-Port": "CMOS", "555 AO-MinVolts": "0.0000", "COM3-Description": "Serial port driver (boost:asio)", "528 AO-Description": "NI DAC", "555 AO-IOChannel": "Dev1/ao6", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "555 AO-TriggerInputLine": "/Dev1/PFI0", "PRIME-ActualInterval-ms": "374.3700", "LudlController-Port": "COM9", "445 Shutter-Demo": "No", "PRIME-Trigger-Expose Out-Mux": "1", "SliceIndex": 1, "PRIME-CCDTemperatureSetPoint": "-15.0000", "405 Shutter-MinVolts": "0.0000", "PRIME-ExposeOutMode": "First Row", "AxisPositions": null, "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Volts": "5.0000", "528-Name": "DA Shutter", "555-State": "1", "XLIGHT Dichroic-Label": "QUAD", "445 AO-Sequenceable": "1", "405-Name": "DA Shutter", "405 AO-IOChannel": "Dev1/ao0", "Core-Camera": "PRIME", "Focus-Name": "Focus", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "405 Shutter-IOChannel": "Dev1/ao8", "640 AO-Demo": "No", "640 Shutter-SupportsTriggering": "0", "445 AO-Demo": "No", "555-DA Device": "555 Shutter", "470 Shutter-Description": "NI DAC", "528 AO-Volts": "0.0000", "COM1-DataBits": "8", "Vincent-D3-Address": "x", "520 AO-SupportsTriggering": "0", "405-Description": "DA device that is used as a shutter", "640 Shutter-MinVolts": "0.0000", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "405 AO-Name": "AnalogIO", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "640 AO-TriggerInputLine": "/Dev1/PFI0", "405 AO-TriggerInputLine": "/Dev1/PFI0", "640-Description": "DA device that is used as a shutter", "445 Shutter-TriggerSampleRate": "100000", "470 Shutter-TriggerSequenceLength": "1000", "COM1-StopBits": "1", "520 Shutter-SupportsTriggering": "0", "405-State": "0", "Core-Initialize": "1", "XLIGHT Emission Wheel-State": "7", "520 AO-Description": "NI DAC", "528 AO-TriggerInputLine": "/Dev1/PFI0", "445 Shutter-MaxVolts": "5.0000", "COM9-Handshaking": "Off", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "XLIGHT Spin motor-Label": "On (spinning)", "PRIME-PP 5 ENABLED": "No", "UUID": "6cac0eeb-61fd-4889-a7af-9f42373f05cf", "PRIME-TriggerMode": "Internal Trigger", "COM1-Handshaking": "Off", "PRIME-Timing-PostTriggerDelayNs": "49344000.0000", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "Focus-Position": "16479.8000", "528 Shutter-Block voltage": "false", "Channel": "Il-T", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "555 Shutter-Demo": "No", "405 Shutter-Demo": "No", "528 AO-Name": "AnalogIO", "520-State": "0", "Core-Galvo": "", "405 Shutter-TriggerSampleRate": "100000", "Focus-FocusStepSize_(nm)": "100", "PRIME-PixelType": "16bit", "COM4-DataBits": "8", "470 AO-IOChannel": "Dev1/ao2", "520 AO-MinVolts": "0.0000", "COM9-Parity": "None", "PRIME-CircularBufferEnabled": "ON", "PRIME-PP 6 Reset": "No", "640 AO-Description": "NI DAC", "Source": "PRIME", "445 AO-MinVolts": "0.0000", "PIEZO-Name": "Stage", "445 AO-Description": "NI DAC", "PRIME-CircularBufferFrameCount": "46", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "520 AO-Volts": "0.0000", "528 Shutter-TriggerSampleRate": "100000", "XPositionUm": 62321, "OpticalPath-State": "4", "PRIME-PP 3 THRESHOLD": "80", "PRIME-PVCAM Version": "3.7.1", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Gain": "1-HDR", "640 Shutter-TriggerSequenceLength": "1000", "528 AO-SupportsTriggering": "0", "445 AO-TriggerInputLine": "/Dev1/PFI0", "OpticalPath-Name": "OpticalPath", "PRIME-PP 4 ENABLED": "Yes", "PRIME-PVCAM Adapter Version": "1.3.29", "COM3-Name": "COM3", "405 AO-MaxVolts": "5.0000", "520 Shutter-Block voltage": "false", "PRIME-Timing-ClearingTimeNs": "49344000.0000", "Core-Focus": "PIEZO", "528 AO-Block voltage": "false", "TE2000-Description": "Nikon TE2000 hub", "528 AO-MinVolts": "0.0000", "COM4-Parity": "None", "Summary": { "Slices": 11, "ChContrastMin": [ 0, 0, 0, 0, 0, 0 ], "PixelAspect": 1, "SlicesFirst": true, "Interval_ms": 0, "Time": "2019-12-17 21:58:39 -0500", "Prefix": "seq_3", "InitialPositionList": [ { "Label": "Pos0", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.435], "Focus": [16480.2], "XYStage": [ 69822.40000000001, 20100.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos1", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.425], "Focus": [16480.3], "XYStage": [ 69004, 20101 ] }, "GridColumnIndex": 0 }, { "Label": "Pos2", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.44], "Focus": [16480.1], "XYStage": [ 63119.5, 20690.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos3", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.405], "Focus": [16480.2], "XYStage": [ 62321, 20690.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos4", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.415], "Focus": [16480], "XYStage": [ 56604.100000000006, 20688.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos5", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.42000000000002], "Focus": [16480.1], "XYStage": [ 56034.200000000004, 20688.800000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos6", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.455], "Focus": [16480], "XYStage": [ 50889.3, 20496.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos7", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.43], "Focus": [16480.2], "XYStage": [ 50101.600000000006, 20496.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos8", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.67000000000002], "Focus": [16479.9], "XYStage": [ 44818.600000000006, 20496.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos9", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.67000000000002], "Focus": [16480], "XYStage": [ 43897.9, 20436.800000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos10", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.675], "Focus": [16479.9], "XYStage": [ 39670.600000000006, 20664.9 ] }, "GridColumnIndex": 0 }, { "Label": "Pos11", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.69], "Focus": [16479.9], "XYStage": [ 38734, 20664.600000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos12", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.695], "Focus": [16480.1], "XYStage": [ 34037.200000000004, 20664.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos13", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.685], "Focus": [16480.1], "XYStage": [ 33152, 20664.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos14", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.69], "Focus": [16480.1], "XYStage": [ 28122.800000000003, 20664.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos15", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.675], "Focus": [16480], "XYStage": [ 27422.9, 20664.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos16", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.64000000000001], "Focus": [16480], "XYStage": [ 23005.2, 20664.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos17", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.69], "Focus": [16479.9], "XYStage": [ 21820.600000000002, 20409.600000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos18", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.41], "Focus": [16480.1], "XYStage": [ 73697.6, -13222.800000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos19", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.455], "Focus": [16480.1], "XYStage": [ 72765.8, -13223 ] }, "GridColumnIndex": 0 }, { "Label": "Pos20", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.4], "Focus": [16480.2], "XYStage": [ 67445.5, -12303.800000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos21", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.415], "Focus": [16480.2], "XYStage": [ 66613.3, -12303.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos22", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.415], "Focus": [16480.1], "XYStage": [ 61819.4, -11936.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos23", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.405], "Focus": [16480.2], "XYStage": [ 61325.700000000004, -11937.1 ] }, "GridColumnIndex": 0 }, { "Label": "Pos24", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.46], "Focus": [16480.2], "XYStage": [ 55377, -10974.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos25", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.445], "Focus": [16480.1], "XYStage": [ 54827, -11214.800000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos26", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.435], "Focus": [16480.2], "XYStage": [ 49937.100000000006, -10919.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos27", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.425], "Focus": [16480.1], "XYStage": [ 48764, -10919.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos28", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.42000000000002], "Focus": [16480.2], "XYStage": [ 43978.4, -10920.400000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos29", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.435], "Focus": [16480.2], "XYStage": [ 43106.3, -10920.400000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos30", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.405], "Focus": [16480.3], "XYStage": [ 38212.4, -10920.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos31", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.445], "Focus": [16480.2], "XYStage": [ 37254.9, -10920.400000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos32", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.44], "Focus": [16480.3], "XYStage": [ 32472.800000000003, -11294.400000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos33", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [166.95000000000002], "Focus": [16480.2], "XYStage": [ 31420.7, -11294.400000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos34", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [166.95000000000002], "Focus": [16480.2], "XYStage": [ 26881.5, -11640 ] }, "GridColumnIndex": 0 }, { "Label": "Pos35", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [166.98], "Focus": [16480.2], "XYStage": [ 25876.100000000002, -11641.1 ] }, "GridColumnIndex": 0 } ], "z-step_um": 10, "GridRow": 0, "BitDepth": 16, "Depth": 2, "Channels": 6, "Source": "Micro-Manager", "ChColors": [ -16711681, -256, -65281, -1, -1, -16776961 ], "MetadataVersion": 10, "KeepShutterOpenChannels": true, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536, 65536 ], "CustomIntervals_ms": [], "Height": 1200, "MicroManagerVersion": "1.4.23 20180219", "GridColumn": 0, "UUID": "8c8f9d20-b128-4577-9f91-89e70f22d319", "PixelType": "GRAY16", "Width": 1200, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\Yan\\20191217-YC113-S5S6", "Frames": 1, "Comment": "", "UserName": "Nikon", "PixelSize_um": 1.1, "ROI": [ 0, 0, 1200, 1200 ], "Date": "2019-12-17", "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC", "P-DAPI-high" ], "KeepShutterOpenSlices": true, "IJType": 1, "ComputerName": "ZadorConfocal2", "Positions": 36, "PositionIndex": 3, "CameraTimeout": 20000, "TimeFirst": false }, "640 AO-Name": "AnalogIO", "Vincent-D3-Command": "Close" }, "FrameKey-0-1-2": { "COM4-StopBits": "1", "528 AO-MaxVolts": "5.0000", "XLIGHT Excitation Wheel-State": "2", "470 Shutter-Name": "AnalogIO", "Core-Shutter": "555", "555 AO-Demo": "No", "XLIGHT Dichroic-State": "2", "XYStage-StepSize-Y": "0.1000", "ZPositionUm": 147.405, "XYStage-StepSize-X": "0.1000", "PRIME-FirmwareVersion": "20.28", "PRIME-ClearCycles": "2", "520-Name": "DA Shutter", "405 Shutter-Name": "AnalogIO", "PIEZO-StepSize": "0.0050", "PRIME-ClearMode": "Pre-Exposure", "470 AO-MinVolts": "0.0000", "405 AO-SupportsTriggering": "1", "CameraChannelIndex": 0, "XYStage-StartSpeed": "500.0000", "COM9-StopBits": "2", "405 AO-Description": "NI DAC", "COM4-DelayBetweenCharsMs": "0.0000", "445 AO-Block voltage": "false", "520 AO-TriggerInputLine": "/Dev1/PFI0", "555 AO-Description": "NI DAC", "520 AO-TriggerSampleRate": "100000", "COM4-Verbose": "1", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM4-Name": "COM4", "405 Shutter-Volts": "0.0000", "Vincent-D3-Port": "COM1", "PRIME-PP 4 MIN ADU AFFECTED": "200", "470 Shutter-MinVolts": "0.0000", "PRIME-PP 3 ENABLED": "Yes", "470 AO-Description": "NI DAC", "PRIME-Description": "PVCAM API device adapter", "520 Shutter-MaxVolts": "5.0000", "520 Shutter-Volts": "0.0000", "528 AO-Sequenceable": "1", "PRIME-Binning": "1x1", "555 Shutter-MaxVolts": "5.0000", "528 Shutter-TriggerSequenceLength": "1000", "405 Shutter-Block voltage": "false", "XLIGHT Spin motor-State": "1", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "Vincent-D3-Time to open (ms)": "35.0000", "Vincent-D3-Time to close (ms)": "35.0000", "445 AO-TriggerSampleRate": "100000", "640 Shutter-IOChannel": "Dev1/ao14", "555 AO-Sequenceable": "1", "520 AO-Name": "AnalogIO", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "470 AO-Block voltage": "false", "COM1-Verbose": "1", "COM9-AnswerTimeout": "10000.0000", "528 Shutter-Name": "AnalogIO", "PRIME-Trigger Timeout (secs)": "10", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "NextFrame": 0, "470 AO-TriggerSequenceLength": "1000", "PRIME-TransposeXY": "0", "445 AO-TriggerSequenceLength": "1000", "COM1-Name": "COM1", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "COM9-DataBits": "8", "COM1-AnswerTimeout": "500.0000", "Camera": "", "TE2000-Version": "V2.50-V2.50-V2.10", "528 Shutter-Volts": "0.0000", "XLIGHT Hub-Name": "XLIGHT Hub", "PIEZO-Description": "Ludl stage driver adapter", "PositionName": "Pos3", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSampleRate": "100000", "PRIME-FanSpeedSetpoint": "High", "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "405 AO-TriggerSampleRate": "100000", "470 Shutter-Block voltage": "false", "470-State": "0", "COM3-Parity": "None", "405 AO-Volts": "0.0000", "PRIME-TransposeMirrorY": "0", "PRIME-ChipName": "GS144BSI", "470 AO-Volts": "0.0000", "PRIME-TransposeMirrorX": "0", "445 Shutter-SupportsTriggering": "0", "470 Shutter-SupportsTriggering": "0", "XLIGHT Hub-Description": "XLIGHT hub", "PRIME-PreampOffLimit": "10000", "470 AO-Sequenceable": "1", "528 Shutter-MaxVolts": "5.0000", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "Core-XYStage": "XYStage", "470 AO-TriggerSampleRate": "100000", "PRIME-Timing-PreTriggerDelayNs": "24672000.0000", "528-DA Device": "528 Shutter", "Focus-Description": "Nikon TE2000 focus adapter", "FilterBlock-State": "5", "520 AO-Demo": "No", "COM3-DelayBetweenCharsMs": "0.0000", "470 Shutter-Volts": "0.0000", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "PRIME-PP 2 THRESHOLD": "125", "PRIME-PP 4 THRESHOLD": "75", "XYStage-Acceleration": "4.0000", "470 Shutter-Demo": "No", "PRIME-Name": "PMPCIECam00", "640-DA Device": "640 Shutter", "405 AO-Block voltage": "false", "ElapsedTime-ms": 140103, "PRIME-Offset": "100", "445 AO-IOChannel": "Dev1/ao1", "640 AO-MaxVolts": "5.0000", "PositionIndex": 3, "PRIME-PP 1 ENABLED": "Yes", "COM1-BaudRate": "9600", "640-State": "0", "COM3-Verbose": "1", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "LudlController-TransmissionDelay": "0", "PRIME-FullWellCapacity": "62000", "PRIME-SMARTStreamingEnabled": "No", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "640 Shutter-Description": "NI DAC", "528-Description": "DA device that is used as a shutter", "COM4-Description": "Serial port driver (boost:asio)", "405 Shutter-Description": "NI DAC", "TE2000-Name": "TE2000", "528 Shutter-Description": "NI DAC", "COM9-Description": "Serial port driver (boost:asio)", "LudlController-Name": "LudlController", "445 Shutter-TriggerSequenceLength": "1000", "PRIME-SerialNumber": "A18B203008", "445 AO-Volts": "0.0000", "PRIME-X-dimension": "1200", "PRIME-ShutterCloseDelay": "0", "XLIGHT Disk slider-State": "2", "Position": "Default", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 AO-IOChannel": "Dev1/ao5", "520 AO-IOChannel": "Dev1/ao4", "528 AO-TriggerSequenceLength": "1000", "405 Shutter-MaxVolts": "5.0000", "470-DA Device": "470 Shutter", "PRIME-FTCapable": "No", "Core-ChannelGroup": "Channels", "555 AO-SupportsTriggering": "0", "PRIME-Y-dimension": "1200", "Frame": 0, "555 Shutter-Sequenceable": "1", "PRIME-Timing-ExposureTimeNs": "9992000.0000", "Core-ImageProcessor": "", "528-State": "0", "520 AO-Block voltage": "false", "XLIGHT Excitation Wheel-Label": "QUAD", "640 Shutter-Demo": "No", "COM3-StopBits": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "520 Shutter-IOChannel": "Dev1/ao11", "Width": 1200, "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-SupportsTriggering": "0", "640 AO-TriggerSampleRate": "100000", "520-Description": "DA device that is used as a shutter", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "405 Shutter-Sequenceable": "1", "405 Shutter-SupportsTriggering": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-TriggerSampleRate": "100000", "640 Shutter-MaxVolts": "5.0000", "555 AO-MaxVolts": "5.0000", "520 AO-TriggerSequenceLength": "1000", "COM3-BaudRate": "9600", "COM9-BaudRate": "9600", "Vincent-D3-Channel #": "Ch. #1", "PRIME-PMode": "Normal", "OpticalPath-Label": "Left(100)", "BitDepth": 16, "555 AO-TriggerSequenceLength": "1000", "445 Shutter-Description": "NI DAC", "405 AO-Sequenceable": "1", "PRIME-AcquisitionMethod": "Callbacks", "555 AO-Name": "AnalogIO", "PRIME-ShutterMode": "Pre-Exposure", "COM3-Handshaking": "Off", "445 AO-SupportsTriggering": "0", "ROI": "0-0-1200-1200", "COM9-Verbose": "1", "PRIME-Color": "OFF", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "PRIME-ShutterOpenDelay": "0", "COM1-Parity": "None", "Slice": 2, "SlicePosition": -30, "COM9-DelayBetweenCharsMs": "0.0000", "555-Name": "DA Shutter", "PIEZO-LudlSingleAxisName": "B", "XLIGHT Emission Wheel-Label": "585/11(T)", "640 Shutter-Name": "AnalogIO", "640 AO-Sequenceable": "1", "520 Shutter-Demo": "No", "528 Shutter-Demo": "No", "470-Name": "DA Shutter", "520-DA Device": "520 Shutter", "XYStage-Description": "Ludl XY stage driver adapter", "445 Shutter-MinVolts": "0.0000", "528 AO-TriggerSampleRate": "100000", "405 AO-MinVolts": "0.0000", "405 Shutter-TriggerSequenceLength": "1000", "Height": 1200, "555 AO-Block voltage": "false", "640 Shutter-Volts": "0.0000", "PixelType": "GRAY16", "445 Shutter-Block voltage": "false", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "520 Shutter-TriggerSequenceLength": "1000", "COM9-Name": "COM9", "Core-SLM": "", "555 AO-Volts": "4.5000", "555 Shutter-Block voltage": "false", "XYStage-Speed": "1800.0000", "520 AO-MaxVolts": "5.0000", "528 Shutter-SupportsTriggering": "0", "COM1-Description": "Serial port driver (boost:asio)", "PRIME-MetadataEnabled": "No", "520 Shutter-MinVolts": "0.0000", "470 Shutter-Sequenceable": "1", "PRIME-PreampDelay": "0", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-MinVolts": "0.0000", "ChannelIndex": 1, "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Core-AutoShutter": "1", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "Exposure-ms": 160, "PRIME-PP 2 ENABLED": "Yes", "640 Shutter-TriggerSampleRate": "100000", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "470 Shutter-IOChannel": "Dev1/ao10", "405 AO-Demo": "No", "PixelSizeUm": 1.1, "470 AO-Demo": "No", "470 Shutter-MaxVolts": "5.0000", "Vincent-D3-Name": "Vincent-D3", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FileName": "img_000000000_Il-T_002.tif", "LudlController-Reset": "Operate", "PRIME-Exposure": "160.00", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "445-Name": "DA Shutter", "555 Shutter-Name": "AnalogIO", "520 Shutter-TriggerSampleRate": "100000", "445 Shutter-IOChannel": "Dev1/ao9", "PRIME-TransposeCorrection": "0", "XYStage-StepSize": "0.1000", "445-Description": "DA device that is used as a shutter", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 AO-IOChannel": "Dev1/ao7", "520 Shutter-Name": "AnalogIO", "COM3-DataBits": "8", "640 AO-Volts": "0.0000", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "555 Shutter-IOChannel": "Dev1/ao13", "WaitInterval": null, "520 Shutter-Sequenceable": "1", "YPositionUm": 20690.300000000003, "445-State": "0", "445-DA Device": "445 Shutter", "PRIME-CircularBufferAutoSize": "ON", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "555 AO-TriggerSampleRate": "100000", "445 Shutter-Volts": "0.0000", "XLIGHT Hub-Port": "COM3", "Core-AutoFocus": "", "COM4-Handshaking": "Off", "528 Shutter-MinVolts": "0.0000", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "445 Shutter-Name": "AnalogIO", "528 Shutter-Sequenceable": "1", "XYStage-TransposeMirrorX": "0", "XYStage-TransposeMirrorY": "0", "PRIME-ReadoutRate": "100MHz 16bit", "XYStage-Name": "Stage", "640 AO-SupportsTriggering": "0", "640-Name": "DA Shutter", "640 Shutter-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "640 AO-Block voltage": "false", "445 Shutter-Sequenceable": "1", "470 AO-MaxVolts": "5.0000", "COM4-BaudRate": "9600", "470-Description": "DA device that is used as a shutter", "520 Shutter-Description": "NI DAC", "PRIME-CCDTemperature": "-14.9600", "Time": "2019-12-17 21:58:47 -0500", "640 AO-TriggerSequenceLength": "1000", "470 AO-Name": "AnalogIO", "XLIGHT Disk slider-Label": "Disk Pos 40um", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "Binning": "1x1", "TE2000-ModelType": "1", "COM3-AnswerTimeout": "500.0000", "555 Shutter-SupportsTriggering": "0", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "TE2000-Port": "COM4", "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Description": "NI DAC", "COM4-AnswerTimeout": "500.0000", "COM1-DelayBetweenCharsMs": "0.0000", "FilterBlock-Label": "Confocal", "445 AO-Name": "AnalogIO", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-Timing-ReadoutTimeNs": "24672000.0000", "528 AO-Demo": "No", "520 AO-Sequenceable": "1", "555-Description": "DA device that is used as a shutter", "405-DA Device": "405 Shutter", "405 AO-TriggerSequenceLength": "1000", "640 AO-MinVolts": "0.0000", "640 Shutter-Block voltage": "false", "FrameIndex": 0, "PRIME-Port": "CMOS", "555 AO-MinVolts": "0.0000", "COM3-Description": "Serial port driver (boost:asio)", "528 AO-Description": "NI DAC", "555 AO-IOChannel": "Dev1/ao6", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "555 AO-TriggerInputLine": "/Dev1/PFI0", "PRIME-ActualInterval-ms": "374.3700", "LudlController-Port": "COM9", "445 Shutter-Demo": "No", "PRIME-Trigger-Expose Out-Mux": "1", "SliceIndex": 2, "PRIME-CCDTemperatureSetPoint": "-15.0000", "405 Shutter-MinVolts": "0.0000", "PRIME-ExposeOutMode": "First Row", "AxisPositions": null, "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Volts": "5.0000", "528-Name": "DA Shutter", "555-State": "1", "XLIGHT Dichroic-Label": "QUAD", "445 AO-Sequenceable": "1", "405-Name": "DA Shutter", "405 AO-IOChannel": "Dev1/ao0", "Core-Camera": "PRIME", "Focus-Name": "Focus", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "405 Shutter-IOChannel": "Dev1/ao8", "640 AO-Demo": "No", "640 Shutter-SupportsTriggering": "0", "445 AO-Demo": "No", "555-DA Device": "555 Shutter", "470 Shutter-Description": "NI DAC", "528 AO-Volts": "0.0000", "COM1-DataBits": "8", "Vincent-D3-Address": "x", "520 AO-SupportsTriggering": "0", "405-Description": "DA device that is used as a shutter", "640 Shutter-MinVolts": "0.0000", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "405 AO-Name": "AnalogIO", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "640 AO-TriggerInputLine": "/Dev1/PFI0", "405 AO-TriggerInputLine": "/Dev1/PFI0", "640-Description": "DA device that is used as a shutter", "445 Shutter-TriggerSampleRate": "100000", "470 Shutter-TriggerSequenceLength": "1000", "COM1-StopBits": "1", "520 Shutter-SupportsTriggering": "0", "405-State": "0", "Core-Initialize": "1", "XLIGHT Emission Wheel-State": "7", "520 AO-Description": "NI DAC", "528 AO-TriggerInputLine": "/Dev1/PFI0", "445 Shutter-MaxVolts": "5.0000", "COM9-Handshaking": "Off", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "XLIGHT Spin motor-Label": "On (spinning)", "PRIME-PP 5 ENABLED": "No", "UUID": "15c9c7c9-3e26-48c8-a61c-f1282bbc016c", "PRIME-TriggerMode": "Internal Trigger", "COM1-Handshaking": "Off", "PRIME-Timing-PostTriggerDelayNs": "49344000.0000", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "Focus-Position": "16479.8000", "528 Shutter-Block voltage": "false", "Channel": "Il-T", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "555 Shutter-Demo": "No", "405 Shutter-Demo": "No", "528 AO-Name": "AnalogIO", "520-State": "0", "Core-Galvo": "", "405 Shutter-TriggerSampleRate": "100000", "Focus-FocusStepSize_(nm)": "100", "PRIME-PixelType": "16bit", "COM4-DataBits": "8", "470 AO-IOChannel": "Dev1/ao2", "520 AO-MinVolts": "0.0000", "COM9-Parity": "None", "PRIME-CircularBufferEnabled": "ON", "PRIME-PP 6 Reset": "No", "640 AO-Description": "NI DAC", "Source": "PRIME", "445 AO-MinVolts": "0.0000", "PIEZO-Name": "Stage", "445 AO-Description": "NI DAC", "PRIME-CircularBufferFrameCount": "46", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "520 AO-Volts": "0.0000", "528 Shutter-TriggerSampleRate": "100000", "XPositionUm": 62321, "OpticalPath-State": "4", "PRIME-PP 3 THRESHOLD": "80", "PRIME-PVCAM Version": "3.7.1", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Gain": "1-HDR", "640 Shutter-TriggerSequenceLength": "1000", "528 AO-SupportsTriggering": "0", "445 AO-TriggerInputLine": "/Dev1/PFI0", "OpticalPath-Name": "OpticalPath", "PRIME-PP 4 ENABLED": "Yes", "PRIME-PVCAM Adapter Version": "1.3.29", "COM3-Name": "COM3", "405 AO-MaxVolts": "5.0000", "520 Shutter-Block voltage": "false", "PRIME-Timing-ClearingTimeNs": "49344000.0000", "Core-Focus": "PIEZO", "528 AO-Block voltage": "false", "TE2000-Description": "Nikon TE2000 hub", "528 AO-MinVolts": "0.0000", "COM4-Parity": "None", "Summary": { "Slices": 11, "ChContrastMin": [ 0, 0, 0, 0, 0, 0 ], "PixelAspect": 1, "SlicesFirst": true, "Interval_ms": 0, "Time": "2019-12-17 21:58:39 -0500", "Prefix": "seq_3", "InitialPositionList": [ { "Label": "Pos0", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.435], "Focus": [16480.2], "XYStage": [ 69822.40000000001, 20100.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos1", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.425], "Focus": [16480.3], "XYStage": [ 69004, 20101 ] }, "GridColumnIndex": 0 }, { "Label": "Pos2", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.44], "Focus": [16480.1], "XYStage": [ 63119.5, 20690.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos3", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.405], "Focus": [16480.2], "XYStage": [ 62321, 20690.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos4", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.415], "Focus": [16480], "XYStage": [ 56604.100000000006, 20688.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos5", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.42000000000002], "Focus": [16480.1], "XYStage": [ 56034.200000000004, 20688.800000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos6", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.455], "Focus": [16480], "XYStage": [ 50889.3, 20496.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos7", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.43], "Focus": [16480.2], "XYStage": [ 50101.600000000006, 20496.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos8", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.67000000000002], "Focus": [16479.9], "XYStage": [ 44818.600000000006, 20496.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos9", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.67000000000002], "Focus": [16480], "XYStage": [ 43897.9, 20436.800000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos10", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.675], "Focus": [16479.9], "XYStage": [ 39670.600000000006, 20664.9 ] }, "GridColumnIndex": 0 }, { "Label": "Pos11", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.69], "Focus": [16479.9], "XYStage": [ 38734, 20664.600000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos12", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.695], "Focus": [16480.1], "XYStage": [ 34037.200000000004, 20664.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos13", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.685], "Focus": [16480.1], "XYStage": [ 33152, 20664.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos14", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.69], "Focus": [16480.1], "XYStage": [ 28122.800000000003, 20664.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos15", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.675], "Focus": [16480], "XYStage": [ 27422.9, 20664.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos16", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.64000000000001], "Focus": [16480], "XYStage": [ 23005.2, 20664.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos17", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.69], "Focus": [16479.9], "XYStage": [ 21820.600000000002, 20409.600000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos18", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.41], "Focus": [16480.1], "XYStage": [ 73697.6, -13222.800000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos19", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.455], "Focus": [16480.1], "XYStage": [ 72765.8, -13223 ] }, "GridColumnIndex": 0 }, { "Label": "Pos20", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.4], "Focus": [16480.2], "XYStage": [ 67445.5, -12303.800000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos21", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.415], "Focus": [16480.2], "XYStage": [ 66613.3, -12303.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos22", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.415], "Focus": [16480.1], "XYStage": [ 61819.4, -11936.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos23", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.405], "Focus": [16480.2], "XYStage": [ 61325.700000000004, -11937.1 ] }, "GridColumnIndex": 0 }, { "Label": "Pos24", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.46], "Focus": [16480.2], "XYStage": [ 55377, -10974.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos25", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.445], "Focus": [16480.1], "XYStage": [ 54827, -11214.800000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos26", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.435], "Focus": [16480.2], "XYStage": [ 49937.100000000006, -10919.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos27", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.425], "Focus": [16480.1], "XYStage": [ 48764, -10919.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos28", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.42000000000002], "Focus": [16480.2], "XYStage": [ 43978.4, -10920.400000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos29", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.435], "Focus": [16480.2], "XYStage": [ 43106.3, -10920.400000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos30", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.405], "Focus": [16480.3], "XYStage": [ 38212.4, -10920.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos31", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.445], "Focus": [16480.2], "XYStage": [ 37254.9, -10920.400000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos32", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.44], "Focus": [16480.3], "XYStage": [ 32472.800000000003, -11294.400000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos33", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [166.95000000000002], "Focus": [16480.2], "XYStage": [ 31420.7, -11294.400000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos34", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [166.95000000000002], "Focus": [16480.2], "XYStage": [ 26881.5, -11640 ] }, "GridColumnIndex": 0 }, { "Label": "Pos35", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [166.98], "Focus": [16480.2], "XYStage": [ 25876.100000000002, -11641.1 ] }, "GridColumnIndex": 0 } ], "z-step_um": 10, "GridRow": 0, "BitDepth": 16, "Depth": 2, "Channels": 6, "Source": "Micro-Manager", "ChColors": [ -16711681, -256, -65281, -1, -1, -16776961 ], "MetadataVersion": 10, "KeepShutterOpenChannels": true, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536, 65536 ], "CustomIntervals_ms": [], "Height": 1200, "MicroManagerVersion": "1.4.23 20180219", "GridColumn": 0, "UUID": "8c8f9d20-b128-4577-9f91-89e70f22d319", "PixelType": "GRAY16", "Width": 1200, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\Yan\\20191217-YC113-S5S6", "Frames": 1, "Comment": "", "UserName": "Nikon", "PixelSize_um": 1.1, "ROI": [ 0, 0, 1200, 1200 ], "Date": "2019-12-17", "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC", "P-DAPI-high" ], "KeepShutterOpenSlices": true, "IJType": 1, "ComputerName": "ZadorConfocal2", "Positions": 36, "PositionIndex": 3, "CameraTimeout": 20000, "TimeFirst": false }, "640 AO-Name": "AnalogIO", "Vincent-D3-Command": "Close" }, "FrameKey-0-1-3": { "COM4-StopBits": "1", "528 AO-MaxVolts": "5.0000", "XLIGHT Excitation Wheel-State": "2", "470 Shutter-Name": "AnalogIO", "Core-Shutter": "555", "555 AO-Demo": "No", "XLIGHT Dichroic-State": "2", "XYStage-StepSize-Y": "0.1000", "ZPositionUm": 157.405, "XYStage-StepSize-X": "0.1000", "PRIME-FirmwareVersion": "20.28", "PRIME-ClearCycles": "2", "520-Name": "DA Shutter", "405 Shutter-Name": "AnalogIO", "PIEZO-StepSize": "0.0050", "PRIME-ClearMode": "Pre-Exposure", "470 AO-MinVolts": "0.0000", "405 AO-SupportsTriggering": "1", "CameraChannelIndex": 0, "XYStage-StartSpeed": "500.0000", "COM9-StopBits": "2", "405 AO-Description": "NI DAC", "COM4-DelayBetweenCharsMs": "0.0000", "445 AO-Block voltage": "false", "520 AO-TriggerInputLine": "/Dev1/PFI0", "555 AO-Description": "NI DAC", "520 AO-TriggerSampleRate": "100000", "COM4-Verbose": "1", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM4-Name": "COM4", "405 Shutter-Volts": "0.0000", "Vincent-D3-Port": "COM1", "PRIME-PP 4 MIN ADU AFFECTED": "200", "470 Shutter-MinVolts": "0.0000", "PRIME-PP 3 ENABLED": "Yes", "470 AO-Description": "NI DAC", "PRIME-Description": "PVCAM API device adapter", "520 Shutter-MaxVolts": "5.0000", "520 Shutter-Volts": "0.0000", "528 AO-Sequenceable": "1", "PRIME-Binning": "1x1", "555 Shutter-MaxVolts": "5.0000", "528 Shutter-TriggerSequenceLength": "1000", "405 Shutter-Block voltage": "false", "XLIGHT Spin motor-State": "1", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "Vincent-D3-Time to open (ms)": "35.0000", "Vincent-D3-Time to close (ms)": "35.0000", "445 AO-TriggerSampleRate": "100000", "640 Shutter-IOChannel": "Dev1/ao14", "555 AO-Sequenceable": "1", "520 AO-Name": "AnalogIO", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "470 AO-Block voltage": "false", "COM1-Verbose": "1", "COM9-AnswerTimeout": "10000.0000", "528 Shutter-Name": "AnalogIO", "PRIME-Trigger Timeout (secs)": "10", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "NextFrame": 0, "470 AO-TriggerSequenceLength": "1000", "PRIME-TransposeXY": "0", "445 AO-TriggerSequenceLength": "1000", "COM1-Name": "COM1", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "COM9-DataBits": "8", "COM1-AnswerTimeout": "500.0000", "Camera": "", "TE2000-Version": "V2.50-V2.50-V2.10", "528 Shutter-Volts": "0.0000", "XLIGHT Hub-Name": "XLIGHT Hub", "PIEZO-Description": "Ludl stage driver adapter", "PositionName": "Pos3", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSampleRate": "100000", "PRIME-FanSpeedSetpoint": "High", "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "405 AO-TriggerSampleRate": "100000", "470 Shutter-Block voltage": "false", "470-State": "0", "COM3-Parity": "None", "405 AO-Volts": "0.0000", "PRIME-TransposeMirrorY": "0", "PRIME-ChipName": "GS144BSI", "470 AO-Volts": "0.0000", "PRIME-TransposeMirrorX": "0", "445 Shutter-SupportsTriggering": "0", "470 Shutter-SupportsTriggering": "0", "XLIGHT Hub-Description": "XLIGHT hub", "PRIME-PreampOffLimit": "10000", "470 AO-Sequenceable": "1", "528 Shutter-MaxVolts": "5.0000", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "Core-XYStage": "XYStage", "470 AO-TriggerSampleRate": "100000", "PRIME-Timing-PreTriggerDelayNs": "24672000.0000", "528-DA Device": "528 Shutter", "Focus-Description": "Nikon TE2000 focus adapter", "FilterBlock-State": "5", "520 AO-Demo": "No", "COM3-DelayBetweenCharsMs": "0.0000", "470 Shutter-Volts": "0.0000", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "PRIME-PP 2 THRESHOLD": "125", "PRIME-PP 4 THRESHOLD": "75", "XYStage-Acceleration": "4.0000", "470 Shutter-Demo": "No", "PRIME-Name": "PMPCIECam00", "640-DA Device": "640 Shutter", "405 AO-Block voltage": "false", "ElapsedTime-ms": 140501, "PRIME-Offset": "100", "445 AO-IOChannel": "Dev1/ao1", "640 AO-MaxVolts": "5.0000", "PositionIndex": 3, "PRIME-PP 1 ENABLED": "Yes", "COM1-BaudRate": "9600", "640-State": "0", "COM3-Verbose": "1", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "LudlController-TransmissionDelay": "0", "PRIME-FullWellCapacity": "62000", "PRIME-SMARTStreamingEnabled": "No", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "640 Shutter-Description": "NI DAC", "528-Description": "DA device that is used as a shutter", "COM4-Description": "Serial port driver (boost:asio)", "405 Shutter-Description": "NI DAC", "TE2000-Name": "TE2000", "528 Shutter-Description": "NI DAC", "COM9-Description": "Serial port driver (boost:asio)", "LudlController-Name": "LudlController", "445 Shutter-TriggerSequenceLength": "1000", "PRIME-SerialNumber": "A18B203008", "445 AO-Volts": "0.0000", "PRIME-X-dimension": "1200", "PRIME-ShutterCloseDelay": "0", "XLIGHT Disk slider-State": "2", "Position": "Default", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 AO-IOChannel": "Dev1/ao5", "520 AO-IOChannel": "Dev1/ao4", "528 AO-TriggerSequenceLength": "1000", "405 Shutter-MaxVolts": "5.0000", "470-DA Device": "470 Shutter", "PRIME-FTCapable": "No", "Core-ChannelGroup": "Channels", "555 AO-SupportsTriggering": "0", "PRIME-Y-dimension": "1200", "Frame": 0, "555 Shutter-Sequenceable": "1", "PRIME-Timing-ExposureTimeNs": "9992000.0000", "Core-ImageProcessor": "", "528-State": "0", "520 AO-Block voltage": "false", "XLIGHT Excitation Wheel-Label": "QUAD", "640 Shutter-Demo": "No", "COM3-StopBits": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "520 Shutter-IOChannel": "Dev1/ao11", "Width": 1200, "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-SupportsTriggering": "0", "640 AO-TriggerSampleRate": "100000", "520-Description": "DA device that is used as a shutter", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "405 Shutter-Sequenceable": "1", "405 Shutter-SupportsTriggering": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-TriggerSampleRate": "100000", "640 Shutter-MaxVolts": "5.0000", "555 AO-MaxVolts": "5.0000", "520 AO-TriggerSequenceLength": "1000", "COM3-BaudRate": "9600", "COM9-BaudRate": "9600", "Vincent-D3-Channel #": "Ch. #1", "PRIME-PMode": "Normal", "OpticalPath-Label": "Left(100)", "BitDepth": 16, "555 AO-TriggerSequenceLength": "1000", "445 Shutter-Description": "NI DAC", "405 AO-Sequenceable": "1", "PRIME-AcquisitionMethod": "Callbacks", "555 AO-Name": "AnalogIO", "PRIME-ShutterMode": "Pre-Exposure", "COM3-Handshaking": "Off", "445 AO-SupportsTriggering": "0", "ROI": "0-0-1200-1200", "COM9-Verbose": "1", "PRIME-Color": "OFF", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "PRIME-ShutterOpenDelay": "0", "COM1-Parity": "None", "Slice": 3, "SlicePosition": -20, "COM9-DelayBetweenCharsMs": "0.0000", "555-Name": "DA Shutter", "PIEZO-LudlSingleAxisName": "B", "XLIGHT Emission Wheel-Label": "585/11(T)", "640 Shutter-Name": "AnalogIO", "640 AO-Sequenceable": "1", "520 Shutter-Demo": "No", "528 Shutter-Demo": "No", "470-Name": "DA Shutter", "520-DA Device": "520 Shutter", "XYStage-Description": "Ludl XY stage driver adapter", "445 Shutter-MinVolts": "0.0000", "528 AO-TriggerSampleRate": "100000", "405 AO-MinVolts": "0.0000", "405 Shutter-TriggerSequenceLength": "1000", "Height": 1200, "555 AO-Block voltage": "false", "640 Shutter-Volts": "0.0000", "PixelType": "GRAY16", "445 Shutter-Block voltage": "false", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "520 Shutter-TriggerSequenceLength": "1000", "COM9-Name": "COM9", "Core-SLM": "", "555 AO-Volts": "4.5000", "555 Shutter-Block voltage": "false", "XYStage-Speed": "1800.0000", "520 AO-MaxVolts": "5.0000", "528 Shutter-SupportsTriggering": "0", "COM1-Description": "Serial port driver (boost:asio)", "PRIME-MetadataEnabled": "No", "520 Shutter-MinVolts": "0.0000", "470 Shutter-Sequenceable": "1", "PRIME-PreampDelay": "0", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-MinVolts": "0.0000", "ChannelIndex": 1, "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Core-AutoShutter": "1", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "Exposure-ms": 160, "PRIME-PP 2 ENABLED": "Yes", "640 Shutter-TriggerSampleRate": "100000", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "470 Shutter-IOChannel": "Dev1/ao10", "405 AO-Demo": "No", "PixelSizeUm": 1.1, "470 AO-Demo": "No", "470 Shutter-MaxVolts": "5.0000", "Vincent-D3-Name": "Vincent-D3", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FileName": "img_000000000_Il-T_003.tif", "LudlController-Reset": "Operate", "PRIME-Exposure": "160.00", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "445-Name": "DA Shutter", "555 Shutter-Name": "AnalogIO", "520 Shutter-TriggerSampleRate": "100000", "445 Shutter-IOChannel": "Dev1/ao9", "PRIME-TransposeCorrection": "0", "XYStage-StepSize": "0.1000", "445-Description": "DA device that is used as a shutter", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 AO-IOChannel": "Dev1/ao7", "520 Shutter-Name": "AnalogIO", "COM3-DataBits": "8", "640 AO-Volts": "0.0000", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "555 Shutter-IOChannel": "Dev1/ao13", "WaitInterval": null, "520 Shutter-Sequenceable": "1", "YPositionUm": 20690.300000000003, "445-State": "0", "445-DA Device": "445 Shutter", "PRIME-CircularBufferAutoSize": "ON", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "555 AO-TriggerSampleRate": "100000", "445 Shutter-Volts": "0.0000", "XLIGHT Hub-Port": "COM3", "Core-AutoFocus": "", "COM4-Handshaking": "Off", "528 Shutter-MinVolts": "0.0000", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "445 Shutter-Name": "AnalogIO", "528 Shutter-Sequenceable": "1", "XYStage-TransposeMirrorX": "0", "XYStage-TransposeMirrorY": "0", "PRIME-ReadoutRate": "100MHz 16bit", "XYStage-Name": "Stage", "640 AO-SupportsTriggering": "0", "640-Name": "DA Shutter", "640 Shutter-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "640 AO-Block voltage": "false", "445 Shutter-Sequenceable": "1", "470 AO-MaxVolts": "5.0000", "COM4-BaudRate": "9600", "470-Description": "DA device that is used as a shutter", "520 Shutter-Description": "NI DAC", "PRIME-CCDTemperature": "-14.9600", "Time": "2019-12-17 21:58:47 -0500", "640 AO-TriggerSequenceLength": "1000", "470 AO-Name": "AnalogIO", "XLIGHT Disk slider-Label": "Disk Pos 40um", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "Binning": "1x1", "TE2000-ModelType": "1", "COM3-AnswerTimeout": "500.0000", "555 Shutter-SupportsTriggering": "0", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "TE2000-Port": "COM4", "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Description": "NI DAC", "COM4-AnswerTimeout": "500.0000", "COM1-DelayBetweenCharsMs": "0.0000", "FilterBlock-Label": "Confocal", "445 AO-Name": "AnalogIO", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-Timing-ReadoutTimeNs": "24672000.0000", "528 AO-Demo": "No", "520 AO-Sequenceable": "1", "555-Description": "DA device that is used as a shutter", "405-DA Device": "405 Shutter", "405 AO-TriggerSequenceLength": "1000", "640 AO-MinVolts": "0.0000", "640 Shutter-Block voltage": "false", "FrameIndex": 0, "PRIME-Port": "CMOS", "555 AO-MinVolts": "0.0000", "COM3-Description": "Serial port driver (boost:asio)", "528 AO-Description": "NI DAC", "555 AO-IOChannel": "Dev1/ao6", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "555 AO-TriggerInputLine": "/Dev1/PFI0", "PRIME-ActualInterval-ms": "374.3700", "LudlController-Port": "COM9", "445 Shutter-Demo": "No", "PRIME-Trigger-Expose Out-Mux": "1", "SliceIndex": 3, "PRIME-CCDTemperatureSetPoint": "-15.0000", "405 Shutter-MinVolts": "0.0000", "PRIME-ExposeOutMode": "First Row", "AxisPositions": null, "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Volts": "5.0000", "528-Name": "DA Shutter", "555-State": "1", "XLIGHT Dichroic-Label": "QUAD", "445 AO-Sequenceable": "1", "405-Name": "DA Shutter", "405 AO-IOChannel": "Dev1/ao0", "Core-Camera": "PRIME", "Focus-Name": "Focus", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "405 Shutter-IOChannel": "Dev1/ao8", "640 AO-Demo": "No", "640 Shutter-SupportsTriggering": "0", "445 AO-Demo": "No", "555-DA Device": "555 Shutter", "470 Shutter-Description": "NI DAC", "528 AO-Volts": "0.0000", "COM1-DataBits": "8", "Vincent-D3-Address": "x", "520 AO-SupportsTriggering": "0", "405-Description": "DA device that is used as a shutter", "640 Shutter-MinVolts": "0.0000", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "405 AO-Name": "AnalogIO", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "640 AO-TriggerInputLine": "/Dev1/PFI0", "405 AO-TriggerInputLine": "/Dev1/PFI0", "640-Description": "DA device that is used as a shutter", "445 Shutter-TriggerSampleRate": "100000", "470 Shutter-TriggerSequenceLength": "1000", "COM1-StopBits": "1", "520 Shutter-SupportsTriggering": "0", "405-State": "0", "Core-Initialize": "1", "XLIGHT Emission Wheel-State": "7", "520 AO-Description": "NI DAC", "528 AO-TriggerInputLine": "/Dev1/PFI0", "445 Shutter-MaxVolts": "5.0000", "COM9-Handshaking": "Off", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "XLIGHT Spin motor-Label": "On (spinning)", "PRIME-PP 5 ENABLED": "No", "UUID": "7f80b57e-2347-400e-bf35-6dbce0c8c7a5", "PRIME-TriggerMode": "Internal Trigger", "COM1-Handshaking": "Off", "PRIME-Timing-PostTriggerDelayNs": "49344000.0000", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "Focus-Position": "16479.8000", "528 Shutter-Block voltage": "false", "Channel": "Il-T", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "555 Shutter-Demo": "No", "405 Shutter-Demo": "No", "528 AO-Name": "AnalogIO", "520-State": "0", "Core-Galvo": "", "405 Shutter-TriggerSampleRate": "100000", "Focus-FocusStepSize_(nm)": "100", "PRIME-PixelType": "16bit", "COM4-DataBits": "8", "470 AO-IOChannel": "Dev1/ao2", "520 AO-MinVolts": "0.0000", "COM9-Parity": "None", "PRIME-CircularBufferEnabled": "ON", "PRIME-PP 6 Reset": "No", "640 AO-Description": "NI DAC", "Source": "PRIME", "445 AO-MinVolts": "0.0000", "PIEZO-Name": "Stage", "445 AO-Description": "NI DAC", "PRIME-CircularBufferFrameCount": "46", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "520 AO-Volts": "0.0000", "528 Shutter-TriggerSampleRate": "100000", "XPositionUm": 62321, "OpticalPath-State": "4", "PRIME-PP 3 THRESHOLD": "80", "PRIME-PVCAM Version": "3.7.1", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Gain": "1-HDR", "640 Shutter-TriggerSequenceLength": "1000", "528 AO-SupportsTriggering": "0", "445 AO-TriggerInputLine": "/Dev1/PFI0", "OpticalPath-Name": "OpticalPath", "PRIME-PP 4 ENABLED": "Yes", "PRIME-PVCAM Adapter Version": "1.3.29", "COM3-Name": "COM3", "405 AO-MaxVolts": "5.0000", "520 Shutter-Block voltage": "false", "PRIME-Timing-ClearingTimeNs": "49344000.0000", "Core-Focus": "PIEZO", "528 AO-Block voltage": "false", "TE2000-Description": "Nikon TE2000 hub", "528 AO-MinVolts": "0.0000", "COM4-Parity": "None", "Summary": { "Slices": 11, "ChContrastMin": [ 0, 0, 0, 0, 0, 0 ], "PixelAspect": 1, "SlicesFirst": true, "Interval_ms": 0, "Time": "2019-12-17 21:58:39 -0500", "Prefix": "seq_3", "InitialPositionList": [ { "Label": "Pos0", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.435], "Focus": [16480.2], "XYStage": [ 69822.40000000001, 20100.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos1", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.425], "Focus": [16480.3], "XYStage": [ 69004, 20101 ] }, "GridColumnIndex": 0 }, { "Label": "Pos2", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.44], "Focus": [16480.1], "XYStage": [ 63119.5, 20690.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos3", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.405], "Focus": [16480.2], "XYStage": [ 62321, 20690.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos4", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.415], "Focus": [16480], "XYStage": [ 56604.100000000006, 20688.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos5", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.42000000000002], "Focus": [16480.1], "XYStage": [ 56034.200000000004, 20688.800000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos6", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.455], "Focus": [16480], "XYStage": [ 50889.3, 20496.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos7", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.43], "Focus": [16480.2], "XYStage": [ 50101.600000000006, 20496.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos8", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.67000000000002], "Focus": [16479.9], "XYStage": [ 44818.600000000006, 20496.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos9", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.67000000000002], "Focus": [16480], "XYStage": [ 43897.9, 20436.800000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos10", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.675], "Focus": [16479.9], "XYStage": [ 39670.600000000006, 20664.9 ] }, "GridColumnIndex": 0 }, { "Label": "Pos11", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.69], "Focus": [16479.9], "XYStage": [ 38734, 20664.600000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos12", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.695], "Focus": [16480.1], "XYStage": [ 34037.200000000004, 20664.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos13", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.685], "Focus": [16480.1], "XYStage": [ 33152, 20664.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos14", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.69], "Focus": [16480.1], "XYStage": [ 28122.800000000003, 20664.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos15", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.675], "Focus": [16480], "XYStage": [ 27422.9, 20664.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos16", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.64000000000001], "Focus": [16480], "XYStage": [ 23005.2, 20664.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos17", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.69], "Focus": [16479.9], "XYStage": [ 21820.600000000002, 20409.600000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos18", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.41], "Focus": [16480.1], "XYStage": [ 73697.6, -13222.800000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos19", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.455], "Focus": [16480.1], "XYStage": [ 72765.8, -13223 ] }, "GridColumnIndex": 0 }, { "Label": "Pos20", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.4], "Focus": [16480.2], "XYStage": [ 67445.5, -12303.800000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos21", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.415], "Focus": [16480.2], "XYStage": [ 66613.3, -12303.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos22", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.415], "Focus": [16480.1], "XYStage": [ 61819.4, -11936.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos23", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.405], "Focus": [16480.2], "XYStage": [ 61325.700000000004, -11937.1 ] }, "GridColumnIndex": 0 }, { "Label": "Pos24", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.46], "Focus": [16480.2], "XYStage": [ 55377, -10974.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos25", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.445], "Focus": [16480.1], "XYStage": [ 54827, -11214.800000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos26", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.435], "Focus": [16480.2], "XYStage": [ 49937.100000000006, -10919.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos27", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.425], "Focus": [16480.1], "XYStage": [ 48764, -10919.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos28", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.42000000000002], "Focus": [16480.2], "XYStage": [ 43978.4, -10920.400000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos29", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.435], "Focus": [16480.2], "XYStage": [ 43106.3, -10920.400000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos30", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.405], "Focus": [16480.3], "XYStage": [ 38212.4, -10920.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos31", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.445], "Focus": [16480.2], "XYStage": [ 37254.9, -10920.400000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos32", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.44], "Focus": [16480.3], "XYStage": [ 32472.800000000003, -11294.400000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos33", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [166.95000000000002], "Focus": [16480.2], "XYStage": [ 31420.7, -11294.400000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos34", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [166.95000000000002], "Focus": [16480.2], "XYStage": [ 26881.5, -11640 ] }, "GridColumnIndex": 0 }, { "Label": "Pos35", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [166.98], "Focus": [16480.2], "XYStage": [ 25876.100000000002, -11641.1 ] }, "GridColumnIndex": 0 } ], "z-step_um": 10, "GridRow": 0, "BitDepth": 16, "Depth": 2, "Channels": 6, "Source": "Micro-Manager", "ChColors": [ -16711681, -256, -65281, -1, -1, -16776961 ], "MetadataVersion": 10, "KeepShutterOpenChannels": true, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536, 65536 ], "CustomIntervals_ms": [], "Height": 1200, "MicroManagerVersion": "1.4.23 20180219", "GridColumn": 0, "UUID": "8c8f9d20-b128-4577-9f91-89e70f22d319", "PixelType": "GRAY16", "Width": 1200, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\Yan\\20191217-YC113-S5S6", "Frames": 1, "Comment": "", "UserName": "Nikon", "PixelSize_um": 1.1, "ROI": [ 0, 0, 1200, 1200 ], "Date": "2019-12-17", "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC", "P-DAPI-high" ], "KeepShutterOpenSlices": true, "IJType": 1, "ComputerName": "ZadorConfocal2", "Positions": 36, "PositionIndex": 3, "CameraTimeout": 20000, "TimeFirst": false }, "640 AO-Name": "AnalogIO", "Vincent-D3-Command": "Close" }, "FrameKey-0-1-4": { "COM4-StopBits": "1", "528 AO-MaxVolts": "5.0000", "XLIGHT Excitation Wheel-State": "2", "470 Shutter-Name": "AnalogIO", "Core-Shutter": "555", "555 AO-Demo": "No", "XLIGHT Dichroic-State": "2", "XYStage-StepSize-Y": "0.1000", "ZPositionUm": 167.405, "XYStage-StepSize-X": "0.1000", "PRIME-FirmwareVersion": "20.28", "PRIME-ClearCycles": "2", "520-Name": "DA Shutter", "405 Shutter-Name": "AnalogIO", "PIEZO-StepSize": "0.0050", "PRIME-ClearMode": "Pre-Exposure", "470 AO-MinVolts": "0.0000", "405 AO-SupportsTriggering": "1", "CameraChannelIndex": 0, "XYStage-StartSpeed": "500.0000", "COM9-StopBits": "2", "405 AO-Description": "NI DAC", "COM4-DelayBetweenCharsMs": "0.0000", "445 AO-Block voltage": "false", "520 AO-TriggerInputLine": "/Dev1/PFI0", "555 AO-Description": "NI DAC", "520 AO-TriggerSampleRate": "100000", "COM4-Verbose": "1", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM4-Name": "COM4", "405 Shutter-Volts": "0.0000", "Vincent-D3-Port": "COM1", "PRIME-PP 4 MIN ADU AFFECTED": "200", "470 Shutter-MinVolts": "0.0000", "PRIME-PP 3 ENABLED": "Yes", "470 AO-Description": "NI DAC", "PRIME-Description": "PVCAM API device adapter", "520 Shutter-MaxVolts": "5.0000", "520 Shutter-Volts": "0.0000", "528 AO-Sequenceable": "1", "PRIME-Binning": "1x1", "555 Shutter-MaxVolts": "5.0000", "528 Shutter-TriggerSequenceLength": "1000", "405 Shutter-Block voltage": "false", "XLIGHT Spin motor-State": "1", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "Vincent-D3-Time to open (ms)": "35.0000", "Vincent-D3-Time to close (ms)": "35.0000", "445 AO-TriggerSampleRate": "100000", "640 Shutter-IOChannel": "Dev1/ao14", "555 AO-Sequenceable": "1", "520 AO-Name": "AnalogIO", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "470 AO-Block voltage": "false", "COM1-Verbose": "1", "COM9-AnswerTimeout": "10000.0000", "528 Shutter-Name": "AnalogIO", "PRIME-Trigger Timeout (secs)": "10", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "NextFrame": 0, "470 AO-TriggerSequenceLength": "1000", "PRIME-TransposeXY": "0", "445 AO-TriggerSequenceLength": "1000", "COM1-Name": "COM1", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "COM9-DataBits": "8", "COM1-AnswerTimeout": "500.0000", "Camera": "", "TE2000-Version": "V2.50-V2.50-V2.10", "528 Shutter-Volts": "0.0000", "XLIGHT Hub-Name": "XLIGHT Hub", "PIEZO-Description": "Ludl stage driver adapter", "PositionName": "Pos3", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSampleRate": "100000", "PRIME-FanSpeedSetpoint": "High", "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "405 AO-TriggerSampleRate": "100000", "470 Shutter-Block voltage": "false", "470-State": "0", "COM3-Parity": "None", "405 AO-Volts": "0.0000", "PRIME-TransposeMirrorY": "0", "PRIME-ChipName": "GS144BSI", "470 AO-Volts": "0.0000", "PRIME-TransposeMirrorX": "0", "445 Shutter-SupportsTriggering": "0", "470 Shutter-SupportsTriggering": "0", "XLIGHT Hub-Description": "XLIGHT hub", "PRIME-PreampOffLimit": "10000", "470 AO-Sequenceable": "1", "528 Shutter-MaxVolts": "5.0000", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "Core-XYStage": "XYStage", "470 AO-TriggerSampleRate": "100000", "PRIME-Timing-PreTriggerDelayNs": "24672000.0000", "528-DA Device": "528 Shutter", "Focus-Description": "Nikon TE2000 focus adapter", "FilterBlock-State": "5", "520 AO-Demo": "No", "COM3-DelayBetweenCharsMs": "0.0000", "470 Shutter-Volts": "0.0000", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "PRIME-PP 2 THRESHOLD": "125", "PRIME-PP 4 THRESHOLD": "75", "XYStage-Acceleration": "4.0000", "470 Shutter-Demo": "No", "PRIME-Name": "PMPCIECam00", "640-DA Device": "640 Shutter", "405 AO-Block voltage": "false", "ElapsedTime-ms": 140901, "PRIME-Offset": "100", "445 AO-IOChannel": "Dev1/ao1", "640 AO-MaxVolts": "5.0000", "PositionIndex": 3, "PRIME-PP 1 ENABLED": "Yes", "COM1-BaudRate": "9600", "640-State": "0", "COM3-Verbose": "1", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "LudlController-TransmissionDelay": "0", "PRIME-FullWellCapacity": "62000", "PRIME-SMARTStreamingEnabled": "No", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "640 Shutter-Description": "NI DAC", "528-Description": "DA device that is used as a shutter", "COM4-Description": "Serial port driver (boost:asio)", "405 Shutter-Description": "NI DAC", "TE2000-Name": "TE2000", "528 Shutter-Description": "NI DAC", "COM9-Description": "Serial port driver (boost:asio)", "LudlController-Name": "LudlController", "445 Shutter-TriggerSequenceLength": "1000", "PRIME-SerialNumber": "A18B203008", "445 AO-Volts": "0.0000", "PRIME-X-dimension": "1200", "PRIME-ShutterCloseDelay": "0", "XLIGHT Disk slider-State": "2", "Position": "Default", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 AO-IOChannel": "Dev1/ao5", "520 AO-IOChannel": "Dev1/ao4", "528 AO-TriggerSequenceLength": "1000", "405 Shutter-MaxVolts": "5.0000", "470-DA Device": "470 Shutter", "PRIME-FTCapable": "No", "Core-ChannelGroup": "Channels", "555 AO-SupportsTriggering": "0", "PRIME-Y-dimension": "1200", "Frame": 0, "555 Shutter-Sequenceable": "1", "PRIME-Timing-ExposureTimeNs": "9992000.0000", "Core-ImageProcessor": "", "528-State": "0", "520 AO-Block voltage": "false", "XLIGHT Excitation Wheel-Label": "QUAD", "640 Shutter-Demo": "No", "COM3-StopBits": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "520 Shutter-IOChannel": "Dev1/ao11", "Width": 1200, "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-SupportsTriggering": "0", "640 AO-TriggerSampleRate": "100000", "520-Description": "DA device that is used as a shutter", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "405 Shutter-Sequenceable": "1", "405 Shutter-SupportsTriggering": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-TriggerSampleRate": "100000", "640 Shutter-MaxVolts": "5.0000", "555 AO-MaxVolts": "5.0000", "520 AO-TriggerSequenceLength": "1000", "COM3-BaudRate": "9600", "COM9-BaudRate": "9600", "Vincent-D3-Channel #": "Ch. #1", "PRIME-PMode": "Normal", "OpticalPath-Label": "Left(100)", "BitDepth": 16, "555 AO-TriggerSequenceLength": "1000", "445 Shutter-Description": "NI DAC", "405 AO-Sequenceable": "1", "PRIME-AcquisitionMethod": "Callbacks", "555 AO-Name": "AnalogIO", "PRIME-ShutterMode": "Pre-Exposure", "COM3-Handshaking": "Off", "445 AO-SupportsTriggering": "0", "ROI": "0-0-1200-1200", "COM9-Verbose": "1", "PRIME-Color": "OFF", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "PRIME-ShutterOpenDelay": "0", "COM1-Parity": "None", "Slice": 4, "SlicePosition": -10, "COM9-DelayBetweenCharsMs": "0.0000", "555-Name": "DA Shutter", "PIEZO-LudlSingleAxisName": "B", "XLIGHT Emission Wheel-Label": "585/11(T)", "640 Shutter-Name": "AnalogIO", "640 AO-Sequenceable": "1", "520 Shutter-Demo": "No", "528 Shutter-Demo": "No", "470-Name": "DA Shutter", "520-DA Device": "520 Shutter", "XYStage-Description": "Ludl XY stage driver adapter", "445 Shutter-MinVolts": "0.0000", "528 AO-TriggerSampleRate": "100000", "405 AO-MinVolts": "0.0000", "405 Shutter-TriggerSequenceLength": "1000", "Height": 1200, "555 AO-Block voltage": "false", "640 Shutter-Volts": "0.0000", "PixelType": "GRAY16", "445 Shutter-Block voltage": "false", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "520 Shutter-TriggerSequenceLength": "1000", "COM9-Name": "COM9", "Core-SLM": "", "555 AO-Volts": "4.5000", "555 Shutter-Block voltage": "false", "XYStage-Speed": "1800.0000", "520 AO-MaxVolts": "5.0000", "528 Shutter-SupportsTriggering": "0", "COM1-Description": "Serial port driver (boost:asio)", "PRIME-MetadataEnabled": "No", "520 Shutter-MinVolts": "0.0000", "470 Shutter-Sequenceable": "1", "PRIME-PreampDelay": "0", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-MinVolts": "0.0000", "ChannelIndex": 1, "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Core-AutoShutter": "1", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "Exposure-ms": 160, "PRIME-PP 2 ENABLED": "Yes", "640 Shutter-TriggerSampleRate": "100000", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "470 Shutter-IOChannel": "Dev1/ao10", "405 AO-Demo": "No", "PixelSizeUm": 1.1, "470 AO-Demo": "No", "470 Shutter-MaxVolts": "5.0000", "Vincent-D3-Name": "Vincent-D3", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FileName": "img_000000000_Il-T_004.tif", "LudlController-Reset": "Operate", "PRIME-Exposure": "160.00", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "445-Name": "DA Shutter", "555 Shutter-Name": "AnalogIO", "520 Shutter-TriggerSampleRate": "100000", "445 Shutter-IOChannel": "Dev1/ao9", "PRIME-TransposeCorrection": "0", "XYStage-StepSize": "0.1000", "445-Description": "DA device that is used as a shutter", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 AO-IOChannel": "Dev1/ao7", "520 Shutter-Name": "AnalogIO", "COM3-DataBits": "8", "640 AO-Volts": "0.0000", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "555 Shutter-IOChannel": "Dev1/ao13", "WaitInterval": null, "520 Shutter-Sequenceable": "1", "YPositionUm": 20690.300000000003, "445-State": "0", "445-DA Device": "445 Shutter", "PRIME-CircularBufferAutoSize": "ON", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "555 AO-TriggerSampleRate": "100000", "445 Shutter-Volts": "0.0000", "XLIGHT Hub-Port": "COM3", "Core-AutoFocus": "", "COM4-Handshaking": "Off", "528 Shutter-MinVolts": "0.0000", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "445 Shutter-Name": "AnalogIO", "528 Shutter-Sequenceable": "1", "XYStage-TransposeMirrorX": "0", "XYStage-TransposeMirrorY": "0", "PRIME-ReadoutRate": "100MHz 16bit", "XYStage-Name": "Stage", "640 AO-SupportsTriggering": "0", "640-Name": "DA Shutter", "640 Shutter-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "640 AO-Block voltage": "false", "445 Shutter-Sequenceable": "1", "470 AO-MaxVolts": "5.0000", "COM4-BaudRate": "9600", "470-Description": "DA device that is used as a shutter", "520 Shutter-Description": "NI DAC", "PRIME-CCDTemperature": "-14.9600", "Time": "2019-12-17 21:58:48 -0500", "640 AO-TriggerSequenceLength": "1000", "470 AO-Name": "AnalogIO", "XLIGHT Disk slider-Label": "Disk Pos 40um", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "Binning": "1x1", "TE2000-ModelType": "1", "COM3-AnswerTimeout": "500.0000", "555 Shutter-SupportsTriggering": "0", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "TE2000-Port": "COM4", "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Description": "NI DAC", "COM4-AnswerTimeout": "500.0000", "COM1-DelayBetweenCharsMs": "0.0000", "FilterBlock-Label": "Confocal", "445 AO-Name": "AnalogIO", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-Timing-ReadoutTimeNs": "24672000.0000", "528 AO-Demo": "No", "520 AO-Sequenceable": "1", "555-Description": "DA device that is used as a shutter", "405-DA Device": "405 Shutter", "405 AO-TriggerSequenceLength": "1000", "640 AO-MinVolts": "0.0000", "640 Shutter-Block voltage": "false", "FrameIndex": 0, "PRIME-Port": "CMOS", "555 AO-MinVolts": "0.0000", "COM3-Description": "Serial port driver (boost:asio)", "528 AO-Description": "NI DAC", "555 AO-IOChannel": "Dev1/ao6", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "555 AO-TriggerInputLine": "/Dev1/PFI0", "PRIME-ActualInterval-ms": "374.3700", "LudlController-Port": "COM9", "445 Shutter-Demo": "No", "PRIME-Trigger-Expose Out-Mux": "1", "SliceIndex": 4, "PRIME-CCDTemperatureSetPoint": "-15.0000", "405 Shutter-MinVolts": "0.0000", "PRIME-ExposeOutMode": "First Row", "AxisPositions": null, "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Volts": "5.0000", "528-Name": "DA Shutter", "555-State": "1", "XLIGHT Dichroic-Label": "QUAD", "445 AO-Sequenceable": "1", "405-Name": "DA Shutter", "405 AO-IOChannel": "Dev1/ao0", "Core-Camera": "PRIME", "Focus-Name": "Focus", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "405 Shutter-IOChannel": "Dev1/ao8", "640 AO-Demo": "No", "640 Shutter-SupportsTriggering": "0", "445 AO-Demo": "No", "555-DA Device": "555 Shutter", "470 Shutter-Description": "NI DAC", "528 AO-Volts": "0.0000", "COM1-DataBits": "8", "Vincent-D3-Address": "x", "520 AO-SupportsTriggering": "0", "405-Description": "DA device that is used as a shutter", "640 Shutter-MinVolts": "0.0000", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "405 AO-Name": "AnalogIO", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "640 AO-TriggerInputLine": "/Dev1/PFI0", "405 AO-TriggerInputLine": "/Dev1/PFI0", "640-Description": "DA device that is used as a shutter", "445 Shutter-TriggerSampleRate": "100000", "470 Shutter-TriggerSequenceLength": "1000", "COM1-StopBits": "1", "520 Shutter-SupportsTriggering": "0", "405-State": "0", "Core-Initialize": "1", "XLIGHT Emission Wheel-State": "7", "520 AO-Description": "NI DAC", "528 AO-TriggerInputLine": "/Dev1/PFI0", "445 Shutter-MaxVolts": "5.0000", "COM9-Handshaking": "Off", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "XLIGHT Spin motor-Label": "On (spinning)", "PRIME-PP 5 ENABLED": "No", "UUID": "2bfc4dd0-91b3-4224-9a4b-897884bbb445", "PRIME-TriggerMode": "Internal Trigger", "COM1-Handshaking": "Off", "PRIME-Timing-PostTriggerDelayNs": "49344000.0000", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "Focus-Position": "16479.8000", "528 Shutter-Block voltage": "false", "Channel": "Il-T", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "555 Shutter-Demo": "No", "405 Shutter-Demo": "No", "528 AO-Name": "AnalogIO", "520-State": "0", "Core-Galvo": "", "405 Shutter-TriggerSampleRate": "100000", "Focus-FocusStepSize_(nm)": "100", "PRIME-PixelType": "16bit", "COM4-DataBits": "8", "470 AO-IOChannel": "Dev1/ao2", "520 AO-MinVolts": "0.0000", "COM9-Parity": "None", "PRIME-CircularBufferEnabled": "ON", "PRIME-PP 6 Reset": "No", "640 AO-Description": "NI DAC", "Source": "PRIME", "445 AO-MinVolts": "0.0000", "PIEZO-Name": "Stage", "445 AO-Description": "NI DAC", "PRIME-CircularBufferFrameCount": "46", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "520 AO-Volts": "0.0000", "528 Shutter-TriggerSampleRate": "100000", "XPositionUm": 62321, "OpticalPath-State": "4", "PRIME-PP 3 THRESHOLD": "80", "PRIME-PVCAM Version": "3.7.1", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Gain": "1-HDR", "640 Shutter-TriggerSequenceLength": "1000", "528 AO-SupportsTriggering": "0", "445 AO-TriggerInputLine": "/Dev1/PFI0", "OpticalPath-Name": "OpticalPath", "PRIME-PP 4 ENABLED": "Yes", "PRIME-PVCAM Adapter Version": "1.3.29", "COM3-Name": "COM3", "405 AO-MaxVolts": "5.0000", "520 Shutter-Block voltage": "false", "PRIME-Timing-ClearingTimeNs": "49344000.0000", "Core-Focus": "PIEZO", "528 AO-Block voltage": "false", "TE2000-Description": "Nikon TE2000 hub", "528 AO-MinVolts": "0.0000", "COM4-Parity": "None", "Summary": { "Slices": 11, "ChContrastMin": [ 0, 0, 0, 0, 0, 0 ], "PixelAspect": 1, "SlicesFirst": true, "Interval_ms": 0, "Time": "2019-12-17 21:58:39 -0500", "Prefix": "seq_3", "InitialPositionList": [ { "Label": "Pos0", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.435], "Focus": [16480.2], "XYStage": [ 69822.40000000001, 20100.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos1", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.425], "Focus": [16480.3], "XYStage": [ 69004, 20101 ] }, "GridColumnIndex": 0 }, { "Label": "Pos2", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.44], "Focus": [16480.1], "XYStage": [ 63119.5, 20690.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos3", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.405], "Focus": [16480.2], "XYStage": [ 62321, 20690.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos4", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.415], "Focus": [16480], "XYStage": [ 56604.100000000006, 20688.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos5", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.42000000000002], "Focus": [16480.1], "XYStage": [ 56034.200000000004, 20688.800000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos6", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.455], "Focus": [16480], "XYStage": [ 50889.3, 20496.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos7", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.43], "Focus": [16480.2], "XYStage": [ 50101.600000000006, 20496.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos8", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.67000000000002], "Focus": [16479.9], "XYStage": [ 44818.600000000006, 20496.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos9", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.67000000000002], "Focus": [16480], "XYStage": [ 43897.9, 20436.800000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos10", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.675], "Focus": [16479.9], "XYStage": [ 39670.600000000006, 20664.9 ] }, "GridColumnIndex": 0 }, { "Label": "Pos11", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.69], "Focus": [16479.9], "XYStage": [ 38734, 20664.600000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos12", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.695], "Focus": [16480.1], "XYStage": [ 34037.200000000004, 20664.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos13", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.685], "Focus": [16480.1], "XYStage": [ 33152, 20664.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos14", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.69], "Focus": [16480.1], "XYStage": [ 28122.800000000003, 20664.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos15", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.675], "Focus": [16480], "XYStage": [ 27422.9, 20664.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos16", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.64000000000001], "Focus": [16480], "XYStage": [ 23005.2, 20664.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos17", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.69], "Focus": [16479.9], "XYStage": [ 21820.600000000002, 20409.600000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos18", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.41], "Focus": [16480.1], "XYStage": [ 73697.6, -13222.800000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos19", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.455], "Focus": [16480.1], "XYStage": [ 72765.8, -13223 ] }, "GridColumnIndex": 0 }, { "Label": "Pos20", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.4], "Focus": [16480.2], "XYStage": [ 67445.5, -12303.800000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos21", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.415], "Focus": [16480.2], "XYStage": [ 66613.3, -12303.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos22", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.415], "Focus": [16480.1], "XYStage": [ 61819.4, -11936.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos23", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.405], "Focus": [16480.2], "XYStage": [ 61325.700000000004, -11937.1 ] }, "GridColumnIndex": 0 }, { "Label": "Pos24", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.46], "Focus": [16480.2], "XYStage": [ 55377, -10974.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos25", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.445], "Focus": [16480.1], "XYStage": [ 54827, -11214.800000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos26", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.435], "Focus": [16480.2], "XYStage": [ 49937.100000000006, -10919.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos27", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.425], "Focus": [16480.1], "XYStage": [ 48764, -10919.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos28", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.42000000000002], "Focus": [16480.2], "XYStage": [ 43978.4, -10920.400000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos29", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.435], "Focus": [16480.2], "XYStage": [ 43106.3, -10920.400000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos30", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.405], "Focus": [16480.3], "XYStage": [ 38212.4, -10920.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos31", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.445], "Focus": [16480.2], "XYStage": [ 37254.9, -10920.400000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos32", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.44], "Focus": [16480.3], "XYStage": [ 32472.800000000003, -11294.400000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos33", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [166.95000000000002], "Focus": [16480.2], "XYStage": [ 31420.7, -11294.400000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos34", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [166.95000000000002], "Focus": [16480.2], "XYStage": [ 26881.5, -11640 ] }, "GridColumnIndex": 0 }, { "Label": "Pos35", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [166.98], "Focus": [16480.2], "XYStage": [ 25876.100000000002, -11641.1 ] }, "GridColumnIndex": 0 } ], "z-step_um": 10, "GridRow": 0, "BitDepth": 16, "Depth": 2, "Channels": 6, "Source": "Micro-Manager", "ChColors": [ -16711681, -256, -65281, -1, -1, -16776961 ], "MetadataVersion": 10, "KeepShutterOpenChannels": true, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536, 65536 ], "CustomIntervals_ms": [], "Height": 1200, "MicroManagerVersion": "1.4.23 20180219", "GridColumn": 0, "UUID": "8c8f9d20-b128-4577-9f91-89e70f22d319", "PixelType": "GRAY16", "Width": 1200, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\Yan\\20191217-YC113-S5S6", "Frames": 1, "Comment": "", "UserName": "Nikon", "PixelSize_um": 1.1, "ROI": [ 0, 0, 1200, 1200 ], "Date": "2019-12-17", "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC", "P-DAPI-high" ], "KeepShutterOpenSlices": true, "IJType": 1, "ComputerName": "ZadorConfocal2", "Positions": 36, "PositionIndex": 3, "CameraTimeout": 20000, "TimeFirst": false }, "640 AO-Name": "AnalogIO", "Vincent-D3-Command": "Close" }, "FrameKey-0-1-5": { "COM4-StopBits": "1", "528 AO-MaxVolts": "5.0000", "XLIGHT Excitation Wheel-State": "2", "470 Shutter-Name": "AnalogIO", "Core-Shutter": "555", "555 AO-Demo": "No", "XLIGHT Dichroic-State": "2", "XYStage-StepSize-Y": "0.1000", "ZPositionUm": 177.405, "XYStage-StepSize-X": "0.1000", "PRIME-FirmwareVersion": "20.28", "PRIME-ClearCycles": "2", "520-Name": "DA Shutter", "405 Shutter-Name": "AnalogIO", "PIEZO-StepSize": "0.0050", "PRIME-ClearMode": "Pre-Exposure", "470 AO-MinVolts": "0.0000", "405 AO-SupportsTriggering": "1", "CameraChannelIndex": 0, "XYStage-StartSpeed": "500.0000", "COM9-StopBits": "2", "405 AO-Description": "NI DAC", "COM4-DelayBetweenCharsMs": "0.0000", "445 AO-Block voltage": "false", "520 AO-TriggerInputLine": "/Dev1/PFI0", "555 AO-Description": "NI DAC", "520 AO-TriggerSampleRate": "100000", "COM4-Verbose": "1", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM4-Name": "COM4", "405 Shutter-Volts": "0.0000", "Vincent-D3-Port": "COM1", "PRIME-PP 4 MIN ADU AFFECTED": "200", "470 Shutter-MinVolts": "0.0000", "PRIME-PP 3 ENABLED": "Yes", "470 AO-Description": "NI DAC", "PRIME-Description": "PVCAM API device adapter", "520 Shutter-MaxVolts": "5.0000", "520 Shutter-Volts": "0.0000", "528 AO-Sequenceable": "1", "PRIME-Binning": "1x1", "555 Shutter-MaxVolts": "5.0000", "528 Shutter-TriggerSequenceLength": "1000", "405 Shutter-Block voltage": "false", "XLIGHT Spin motor-State": "1", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "Vincent-D3-Time to open (ms)": "35.0000", "Vincent-D3-Time to close (ms)": "35.0000", "445 AO-TriggerSampleRate": "100000", "640 Shutter-IOChannel": "Dev1/ao14", "555 AO-Sequenceable": "1", "520 AO-Name": "AnalogIO", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "470 AO-Block voltage": "false", "COM1-Verbose": "1", "COM9-AnswerTimeout": "10000.0000", "528 Shutter-Name": "AnalogIO", "PRIME-Trigger Timeout (secs)": "10", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "NextFrame": 0, "470 AO-TriggerSequenceLength": "1000", "PRIME-TransposeXY": "0", "445 AO-TriggerSequenceLength": "1000", "COM1-Name": "COM1", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "COM9-DataBits": "8", "COM1-AnswerTimeout": "500.0000", "Camera": "", "TE2000-Version": "V2.50-V2.50-V2.10", "528 Shutter-Volts": "0.0000", "XLIGHT Hub-Name": "XLIGHT Hub", "PIEZO-Description": "Ludl stage driver adapter", "PositionName": "Pos3", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSampleRate": "100000", "PRIME-FanSpeedSetpoint": "High", "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "405 AO-TriggerSampleRate": "100000", "470 Shutter-Block voltage": "false", "470-State": "0", "COM3-Parity": "None", "405 AO-Volts": "0.0000", "PRIME-TransposeMirrorY": "0", "PRIME-ChipName": "GS144BSI", "470 AO-Volts": "0.0000", "PRIME-TransposeMirrorX": "0", "445 Shutter-SupportsTriggering": "0", "470 Shutter-SupportsTriggering": "0", "XLIGHT Hub-Description": "XLIGHT hub", "PRIME-PreampOffLimit": "10000", "470 AO-Sequenceable": "1", "528 Shutter-MaxVolts": "5.0000", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "Core-XYStage": "XYStage", "470 AO-TriggerSampleRate": "100000", "PRIME-Timing-PreTriggerDelayNs": "24672000.0000", "528-DA Device": "528 Shutter", "Focus-Description": "Nikon TE2000 focus adapter", "FilterBlock-State": "5", "520 AO-Demo": "No", "COM3-DelayBetweenCharsMs": "0.0000", "470 Shutter-Volts": "0.0000", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "PRIME-PP 2 THRESHOLD": "125", "PRIME-PP 4 THRESHOLD": "75", "XYStage-Acceleration": "4.0000", "470 Shutter-Demo": "No", "PRIME-Name": "PMPCIECam00", "640-DA Device": "640 Shutter", "405 AO-Block voltage": "false", "ElapsedTime-ms": 141301, "PRIME-Offset": "100", "445 AO-IOChannel": "Dev1/ao1", "640 AO-MaxVolts": "5.0000", "PositionIndex": 3, "PRIME-PP 1 ENABLED": "Yes", "COM1-BaudRate": "9600", "640-State": "0", "COM3-Verbose": "1", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "LudlController-TransmissionDelay": "0", "PRIME-FullWellCapacity": "62000", "PRIME-SMARTStreamingEnabled": "No", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "640 Shutter-Description": "NI DAC", "528-Description": "DA device that is used as a shutter", "COM4-Description": "Serial port driver (boost:asio)", "405 Shutter-Description": "NI DAC", "TE2000-Name": "TE2000", "528 Shutter-Description": "NI DAC", "COM9-Description": "Serial port driver (boost:asio)", "LudlController-Name": "LudlController", "445 Shutter-TriggerSequenceLength": "1000", "PRIME-SerialNumber": "A18B203008", "445 AO-Volts": "0.0000", "PRIME-X-dimension": "1200", "PRIME-ShutterCloseDelay": "0", "XLIGHT Disk slider-State": "2", "Position": "Default", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 AO-IOChannel": "Dev1/ao5", "520 AO-IOChannel": "Dev1/ao4", "528 AO-TriggerSequenceLength": "1000", "405 Shutter-MaxVolts": "5.0000", "470-DA Device": "470 Shutter", "PRIME-FTCapable": "No", "Core-ChannelGroup": "Channels", "555 AO-SupportsTriggering": "0", "PRIME-Y-dimension": "1200", "Frame": 0, "555 Shutter-Sequenceable": "1", "PRIME-Timing-ExposureTimeNs": "9992000.0000", "Core-ImageProcessor": "", "528-State": "0", "520 AO-Block voltage": "false", "XLIGHT Excitation Wheel-Label": "QUAD", "640 Shutter-Demo": "No", "COM3-StopBits": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "520 Shutter-IOChannel": "Dev1/ao11", "Width": 1200, "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-SupportsTriggering": "0", "640 AO-TriggerSampleRate": "100000", "520-Description": "DA device that is used as a shutter", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "405 Shutter-Sequenceable": "1", "405 Shutter-SupportsTriggering": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-TriggerSampleRate": "100000", "640 Shutter-MaxVolts": "5.0000", "555 AO-MaxVolts": "5.0000", "520 AO-TriggerSequenceLength": "1000", "COM3-BaudRate": "9600", "COM9-BaudRate": "9600", "Vincent-D3-Channel #": "Ch. #1", "PRIME-PMode": "Normal", "OpticalPath-Label": "Left(100)", "BitDepth": 16, "555 AO-TriggerSequenceLength": "1000", "445 Shutter-Description": "NI DAC", "405 AO-Sequenceable": "1", "PRIME-AcquisitionMethod": "Callbacks", "555 AO-Name": "AnalogIO", "PRIME-ShutterMode": "Pre-Exposure", "COM3-Handshaking": "Off", "445 AO-SupportsTriggering": "0", "ROI": "0-0-1200-1200", "COM9-Verbose": "1", "PRIME-Color": "OFF", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "PRIME-ShutterOpenDelay": "0", "COM1-Parity": "None", "Slice": 5, "SlicePosition": 0, "COM9-DelayBetweenCharsMs": "0.0000", "555-Name": "DA Shutter", "PIEZO-LudlSingleAxisName": "B", "XLIGHT Emission Wheel-Label": "585/11(T)", "640 Shutter-Name": "AnalogIO", "640 AO-Sequenceable": "1", "520 Shutter-Demo": "No", "528 Shutter-Demo": "No", "470-Name": "DA Shutter", "520-DA Device": "520 Shutter", "XYStage-Description": "Ludl XY stage driver adapter", "445 Shutter-MinVolts": "0.0000", "528 AO-TriggerSampleRate": "100000", "405 AO-MinVolts": "0.0000", "405 Shutter-TriggerSequenceLength": "1000", "Height": 1200, "555 AO-Block voltage": "false", "640 Shutter-Volts": "0.0000", "PixelType": "GRAY16", "445 Shutter-Block voltage": "false", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "520 Shutter-TriggerSequenceLength": "1000", "COM9-Name": "COM9", "Core-SLM": "", "555 AO-Volts": "4.5000", "555 Shutter-Block voltage": "false", "XYStage-Speed": "1800.0000", "520 AO-MaxVolts": "5.0000", "528 Shutter-SupportsTriggering": "0", "COM1-Description": "Serial port driver (boost:asio)", "PRIME-MetadataEnabled": "No", "520 Shutter-MinVolts": "0.0000", "470 Shutter-Sequenceable": "1", "PRIME-PreampDelay": "0", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-MinVolts": "0.0000", "ChannelIndex": 1, "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Core-AutoShutter": "1", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "Exposure-ms": 160, "PRIME-PP 2 ENABLED": "Yes", "640 Shutter-TriggerSampleRate": "100000", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "470 Shutter-IOChannel": "Dev1/ao10", "405 AO-Demo": "No", "PixelSizeUm": 1.1, "470 AO-Demo": "No", "470 Shutter-MaxVolts": "5.0000", "Vincent-D3-Name": "Vincent-D3", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FileName": "img_000000000_Il-T_005.tif", "LudlController-Reset": "Operate", "PRIME-Exposure": "160.00", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "445-Name": "DA Shutter", "555 Shutter-Name": "AnalogIO", "520 Shutter-TriggerSampleRate": "100000", "445 Shutter-IOChannel": "Dev1/ao9", "PRIME-TransposeCorrection": "0", "XYStage-StepSize": "0.1000", "445-Description": "DA device that is used as a shutter", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 AO-IOChannel": "Dev1/ao7", "520 Shutter-Name": "AnalogIO", "COM3-DataBits": "8", "640 AO-Volts": "0.0000", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "555 Shutter-IOChannel": "Dev1/ao13", "WaitInterval": null, "520 Shutter-Sequenceable": "1", "YPositionUm": 20690.300000000003, "445-State": "0", "445-DA Device": "445 Shutter", "PRIME-CircularBufferAutoSize": "ON", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "555 AO-TriggerSampleRate": "100000", "445 Shutter-Volts": "0.0000", "XLIGHT Hub-Port": "COM3", "Core-AutoFocus": "", "COM4-Handshaking": "Off", "528 Shutter-MinVolts": "0.0000", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "445 Shutter-Name": "AnalogIO", "528 Shutter-Sequenceable": "1", "XYStage-TransposeMirrorX": "0", "XYStage-TransposeMirrorY": "0", "PRIME-ReadoutRate": "100MHz 16bit", "XYStage-Name": "Stage", "640 AO-SupportsTriggering": "0", "640-Name": "DA Shutter", "640 Shutter-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "640 AO-Block voltage": "false", "445 Shutter-Sequenceable": "1", "470 AO-MaxVolts": "5.0000", "COM4-BaudRate": "9600", "470-Description": "DA device that is used as a shutter", "520 Shutter-Description": "NI DAC", "PRIME-CCDTemperature": "-14.9600", "Time": "2019-12-17 21:58:48 -0500", "640 AO-TriggerSequenceLength": "1000", "470 AO-Name": "AnalogIO", "XLIGHT Disk slider-Label": "Disk Pos 40um", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "Binning": "1x1", "TE2000-ModelType": "1", "COM3-AnswerTimeout": "500.0000", "555 Shutter-SupportsTriggering": "0", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "TE2000-Port": "COM4", "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Description": "NI DAC", "COM4-AnswerTimeout": "500.0000", "COM1-DelayBetweenCharsMs": "0.0000", "FilterBlock-Label": "Confocal", "445 AO-Name": "AnalogIO", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-Timing-ReadoutTimeNs": "24672000.0000", "528 AO-Demo": "No", "520 AO-Sequenceable": "1", "555-Description": "DA device that is used as a shutter", "405-DA Device": "405 Shutter", "405 AO-TriggerSequenceLength": "1000", "640 AO-MinVolts": "0.0000", "640 Shutter-Block voltage": "false", "FrameIndex": 0, "PRIME-Port": "CMOS", "555 AO-MinVolts": "0.0000", "COM3-Description": "Serial port driver (boost:asio)", "528 AO-Description": "NI DAC", "555 AO-IOChannel": "Dev1/ao6", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "555 AO-TriggerInputLine": "/Dev1/PFI0", "PRIME-ActualInterval-ms": "374.3700", "LudlController-Port": "COM9", "445 Shutter-Demo": "No", "PRIME-Trigger-Expose Out-Mux": "1", "SliceIndex": 5, "PRIME-CCDTemperatureSetPoint": "-15.0000", "405 Shutter-MinVolts": "0.0000", "PRIME-ExposeOutMode": "First Row", "AxisPositions": null, "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Volts": "5.0000", "528-Name": "DA Shutter", "555-State": "1", "XLIGHT Dichroic-Label": "QUAD", "445 AO-Sequenceable": "1", "405-Name": "DA Shutter", "405 AO-IOChannel": "Dev1/ao0", "Core-Camera": "PRIME", "Focus-Name": "Focus", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "405 Shutter-IOChannel": "Dev1/ao8", "640 AO-Demo": "No", "640 Shutter-SupportsTriggering": "0", "445 AO-Demo": "No", "555-DA Device": "555 Shutter", "470 Shutter-Description": "NI DAC", "528 AO-Volts": "0.0000", "COM1-DataBits": "8", "Vincent-D3-Address": "x", "520 AO-SupportsTriggering": "0", "405-Description": "DA device that is used as a shutter", "640 Shutter-MinVolts": "0.0000", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "405 AO-Name": "AnalogIO", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "640 AO-TriggerInputLine": "/Dev1/PFI0", "405 AO-TriggerInputLine": "/Dev1/PFI0", "640-Description": "DA device that is used as a shutter", "445 Shutter-TriggerSampleRate": "100000", "470 Shutter-TriggerSequenceLength": "1000", "COM1-StopBits": "1", "520 Shutter-SupportsTriggering": "0", "405-State": "0", "Core-Initialize": "1", "XLIGHT Emission Wheel-State": "7", "520 AO-Description": "NI DAC", "528 AO-TriggerInputLine": "/Dev1/PFI0", "445 Shutter-MaxVolts": "5.0000", "COM9-Handshaking": "Off", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "XLIGHT Spin motor-Label": "On (spinning)", "PRIME-PP 5 ENABLED": "No", "UUID": "7abde14d-724f-4a9a-b25b-684be2739339", "PRIME-TriggerMode": "Internal Trigger", "COM1-Handshaking": "Off", "PRIME-Timing-PostTriggerDelayNs": "49344000.0000", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "Focus-Position": "16479.8000", "528 Shutter-Block voltage": "false", "Channel": "Il-T", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "555 Shutter-Demo": "No", "405 Shutter-Demo": "No", "528 AO-Name": "AnalogIO", "520-State": "0", "Core-Galvo": "", "405 Shutter-TriggerSampleRate": "100000", "Focus-FocusStepSize_(nm)": "100", "PRIME-PixelType": "16bit", "COM4-DataBits": "8", "470 AO-IOChannel": "Dev1/ao2", "520 AO-MinVolts": "0.0000", "COM9-Parity": "None", "PRIME-CircularBufferEnabled": "ON", "PRIME-PP 6 Reset": "No", "640 AO-Description": "NI DAC", "Source": "PRIME", "445 AO-MinVolts": "0.0000", "PIEZO-Name": "Stage", "445 AO-Description": "NI DAC", "PRIME-CircularBufferFrameCount": "46", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "520 AO-Volts": "0.0000", "528 Shutter-TriggerSampleRate": "100000", "XPositionUm": 62321, "OpticalPath-State": "4", "PRIME-PP 3 THRESHOLD": "80", "PRIME-PVCAM Version": "3.7.1", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Gain": "1-HDR", "640 Shutter-TriggerSequenceLength": "1000", "528 AO-SupportsTriggering": "0", "445 AO-TriggerInputLine": "/Dev1/PFI0", "OpticalPath-Name": "OpticalPath", "PRIME-PP 4 ENABLED": "Yes", "PRIME-PVCAM Adapter Version": "1.3.29", "COM3-Name": "COM3", "405 AO-MaxVolts": "5.0000", "520 Shutter-Block voltage": "false", "PRIME-Timing-ClearingTimeNs": "49344000.0000", "Core-Focus": "PIEZO", "528 AO-Block voltage": "false", "TE2000-Description": "Nikon TE2000 hub", "528 AO-MinVolts": "0.0000", "COM4-Parity": "None", "Summary": { "Slices": 11, "ChContrastMin": [ 0, 0, 0, 0, 0, 0 ], "PixelAspect": 1, "SlicesFirst": true, "Interval_ms": 0, "Time": "2019-12-17 21:58:39 -0500", "Prefix": "seq_3", "InitialPositionList": [ { "Label": "Pos0", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.435], "Focus": [16480.2], "XYStage": [ 69822.40000000001, 20100.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos1", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.425], "Focus": [16480.3], "XYStage": [ 69004, 20101 ] }, "GridColumnIndex": 0 }, { "Label": "Pos2", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.44], "Focus": [16480.1], "XYStage": [ 63119.5, 20690.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos3", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.405], "Focus": [16480.2], "XYStage": [ 62321, 20690.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos4", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.415], "Focus": [16480], "XYStage": [ 56604.100000000006, 20688.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos5", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.42000000000002], "Focus": [16480.1], "XYStage": [ 56034.200000000004, 20688.800000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos6", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.455], "Focus": [16480], "XYStage": [ 50889.3, 20496.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos7", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.43], "Focus": [16480.2], "XYStage": [ 50101.600000000006, 20496.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos8", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.67000000000002], "Focus": [16479.9], "XYStage": [ 44818.600000000006, 20496.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos9", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.67000000000002], "Focus": [16480], "XYStage": [ 43897.9, 20436.800000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos10", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.675], "Focus": [16479.9], "XYStage": [ 39670.600000000006, 20664.9 ] }, "GridColumnIndex": 0 }, { "Label": "Pos11", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.69], "Focus": [16479.9], "XYStage": [ 38734, 20664.600000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos12", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.695], "Focus": [16480.1], "XYStage": [ 34037.200000000004, 20664.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos13", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.685], "Focus": [16480.1], "XYStage": [ 33152, 20664.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos14", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.69], "Focus": [16480.1], "XYStage": [ 28122.800000000003, 20664.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos15", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.675], "Focus": [16480], "XYStage": [ 27422.9, 20664.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos16", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.64000000000001], "Focus": [16480], "XYStage": [ 23005.2, 20664.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos17", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.69], "Focus": [16479.9], "XYStage": [ 21820.600000000002, 20409.600000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos18", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.41], "Focus": [16480.1], "XYStage": [ 73697.6, -13222.800000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos19", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.455], "Focus": [16480.1], "XYStage": [ 72765.8, -13223 ] }, "GridColumnIndex": 0 }, { "Label": "Pos20", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.4], "Focus": [16480.2], "XYStage": [ 67445.5, -12303.800000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos21", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.415], "Focus": [16480.2], "XYStage": [ 66613.3, -12303.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos22", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.415], "Focus": [16480.1], "XYStage": [ 61819.4, -11936.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos23", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.405], "Focus": [16480.2], "XYStage": [ 61325.700000000004, -11937.1 ] }, "GridColumnIndex": 0 }, { "Label": "Pos24", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.46], "Focus": [16480.2], "XYStage": [ 55377, -10974.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos25", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.445], "Focus": [16480.1], "XYStage": [ 54827, -11214.800000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos26", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.435], "Focus": [16480.2], "XYStage": [ 49937.100000000006, -10919.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos27", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.425], "Focus": [16480.1], "XYStage": [ 48764, -10919.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos28", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.42000000000002], "Focus": [16480.2], "XYStage": [ 43978.4, -10920.400000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos29", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.435], "Focus": [16480.2], "XYStage": [ 43106.3, -10920.400000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos30", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.405], "Focus": [16480.3], "XYStage": [ 38212.4, -10920.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos31", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.445], "Focus": [16480.2], "XYStage": [ 37254.9, -10920.400000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos32", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.44], "Focus": [16480.3], "XYStage": [ 32472.800000000003, -11294.400000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos33", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [166.95000000000002], "Focus": [16480.2], "XYStage": [ 31420.7, -11294.400000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos34", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [166.95000000000002], "Focus": [16480.2], "XYStage": [ 26881.5, -11640 ] }, "GridColumnIndex": 0 }, { "Label": "Pos35", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [166.98], "Focus": [16480.2], "XYStage": [ 25876.100000000002, -11641.1 ] }, "GridColumnIndex": 0 } ], "z-step_um": 10, "GridRow": 0, "BitDepth": 16, "Depth": 2, "Channels": 6, "Source": "Micro-Manager", "ChColors": [ -16711681, -256, -65281, -1, -1, -16776961 ], "MetadataVersion": 10, "KeepShutterOpenChannels": true, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536, 65536 ], "CustomIntervals_ms": [], "Height": 1200, "MicroManagerVersion": "1.4.23 20180219", "GridColumn": 0, "UUID": "8c8f9d20-b128-4577-9f91-89e70f22d319", "PixelType": "GRAY16", "Width": 1200, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\Yan\\20191217-YC113-S5S6", "Frames": 1, "Comment": "", "UserName": "Nikon", "PixelSize_um": 1.1, "ROI": [ 0, 0, 1200, 1200 ], "Date": "2019-12-17", "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC", "P-DAPI-high" ], "KeepShutterOpenSlices": true, "IJType": 1, "ComputerName": "ZadorConfocal2", "Positions": 36, "PositionIndex": 3, "CameraTimeout": 20000, "TimeFirst": false }, "640 AO-Name": "AnalogIO", "Vincent-D3-Command": "Close" }, "FrameKey-0-1-6": { "COM4-StopBits": "1", "528 AO-MaxVolts": "5.0000", "XLIGHT Excitation Wheel-State": "2", "470 Shutter-Name": "AnalogIO", "Core-Shutter": "555", "555 AO-Demo": "No", "XLIGHT Dichroic-State": "2", "XYStage-StepSize-Y": "0.1000", "ZPositionUm": 187.405, "XYStage-StepSize-X": "0.1000", "PRIME-FirmwareVersion": "20.28", "PRIME-ClearCycles": "2", "520-Name": "DA Shutter", "405 Shutter-Name": "AnalogIO", "PIEZO-StepSize": "0.0050", "PRIME-ClearMode": "Pre-Exposure", "470 AO-MinVolts": "0.0000", "405 AO-SupportsTriggering": "1", "CameraChannelIndex": 0, "XYStage-StartSpeed": "500.0000", "COM9-StopBits": "2", "405 AO-Description": "NI DAC", "COM4-DelayBetweenCharsMs": "0.0000", "445 AO-Block voltage": "false", "520 AO-TriggerInputLine": "/Dev1/PFI0", "555 AO-Description": "NI DAC", "520 AO-TriggerSampleRate": "100000", "COM4-Verbose": "1", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM4-Name": "COM4", "405 Shutter-Volts": "0.0000", "Vincent-D3-Port": "COM1", "PRIME-PP 4 MIN ADU AFFECTED": "200", "470 Shutter-MinVolts": "0.0000", "PRIME-PP 3 ENABLED": "Yes", "470 AO-Description": "NI DAC", "PRIME-Description": "PVCAM API device adapter", "520 Shutter-MaxVolts": "5.0000", "520 Shutter-Volts": "0.0000", "528 AO-Sequenceable": "1", "PRIME-Binning": "1x1", "555 Shutter-MaxVolts": "5.0000", "528 Shutter-TriggerSequenceLength": "1000", "405 Shutter-Block voltage": "false", "XLIGHT Spin motor-State": "1", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "Vincent-D3-Time to open (ms)": "35.0000", "Vincent-D3-Time to close (ms)": "35.0000", "445 AO-TriggerSampleRate": "100000", "640 Shutter-IOChannel": "Dev1/ao14", "555 AO-Sequenceable": "1", "520 AO-Name": "AnalogIO", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "470 AO-Block voltage": "false", "COM1-Verbose": "1", "COM9-AnswerTimeout": "10000.0000", "528 Shutter-Name": "AnalogIO", "PRIME-Trigger Timeout (secs)": "10", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "NextFrame": 0, "470 AO-TriggerSequenceLength": "1000", "PRIME-TransposeXY": "0", "445 AO-TriggerSequenceLength": "1000", "COM1-Name": "COM1", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "COM9-DataBits": "8", "COM1-AnswerTimeout": "500.0000", "Camera": "", "TE2000-Version": "V2.50-V2.50-V2.10", "528 Shutter-Volts": "0.0000", "XLIGHT Hub-Name": "XLIGHT Hub", "PIEZO-Description": "Ludl stage driver adapter", "PositionName": "Pos3", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSampleRate": "100000", "PRIME-FanSpeedSetpoint": "High", "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "405 AO-TriggerSampleRate": "100000", "470 Shutter-Block voltage": "false", "470-State": "0", "COM3-Parity": "None", "405 AO-Volts": "0.0000", "PRIME-TransposeMirrorY": "0", "PRIME-ChipName": "GS144BSI", "470 AO-Volts": "0.0000", "PRIME-TransposeMirrorX": "0", "445 Shutter-SupportsTriggering": "0", "470 Shutter-SupportsTriggering": "0", "XLIGHT Hub-Description": "XLIGHT hub", "PRIME-PreampOffLimit": "10000", "470 AO-Sequenceable": "1", "528 Shutter-MaxVolts": "5.0000", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "Core-XYStage": "XYStage", "470 AO-TriggerSampleRate": "100000", "PRIME-Timing-PreTriggerDelayNs": "24672000.0000", "528-DA Device": "528 Shutter", "Focus-Description": "Nikon TE2000 focus adapter", "FilterBlock-State": "5", "520 AO-Demo": "No", "COM3-DelayBetweenCharsMs": "0.0000", "470 Shutter-Volts": "0.0000", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "PRIME-PP 2 THRESHOLD": "125", "PRIME-PP 4 THRESHOLD": "75", "XYStage-Acceleration": "4.0000", "470 Shutter-Demo": "No", "PRIME-Name": "PMPCIECam00", "640-DA Device": "640 Shutter", "405 AO-Block voltage": "false", "ElapsedTime-ms": 141701, "PRIME-Offset": "100", "445 AO-IOChannel": "Dev1/ao1", "640 AO-MaxVolts": "5.0000", "PositionIndex": 3, "PRIME-PP 1 ENABLED": "Yes", "COM1-BaudRate": "9600", "640-State": "0", "COM3-Verbose": "1", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "LudlController-TransmissionDelay": "0", "PRIME-FullWellCapacity": "62000", "PRIME-SMARTStreamingEnabled": "No", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "640 Shutter-Description": "NI DAC", "528-Description": "DA device that is used as a shutter", "COM4-Description": "Serial port driver (boost:asio)", "405 Shutter-Description": "NI DAC", "TE2000-Name": "TE2000", "528 Shutter-Description": "NI DAC", "COM9-Description": "Serial port driver (boost:asio)", "LudlController-Name": "LudlController", "445 Shutter-TriggerSequenceLength": "1000", "PRIME-SerialNumber": "A18B203008", "445 AO-Volts": "0.0000", "PRIME-X-dimension": "1200", "PRIME-ShutterCloseDelay": "0", "XLIGHT Disk slider-State": "2", "Position": "Default", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 AO-IOChannel": "Dev1/ao5", "520 AO-IOChannel": "Dev1/ao4", "528 AO-TriggerSequenceLength": "1000", "405 Shutter-MaxVolts": "5.0000", "470-DA Device": "470 Shutter", "PRIME-FTCapable": "No", "Core-ChannelGroup": "Channels", "555 AO-SupportsTriggering": "0", "PRIME-Y-dimension": "1200", "Frame": 0, "555 Shutter-Sequenceable": "1", "PRIME-Timing-ExposureTimeNs": "9992000.0000", "Core-ImageProcessor": "", "528-State": "0", "520 AO-Block voltage": "false", "XLIGHT Excitation Wheel-Label": "QUAD", "640 Shutter-Demo": "No", "COM3-StopBits": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "520 Shutter-IOChannel": "Dev1/ao11", "Width": 1200, "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-SupportsTriggering": "0", "640 AO-TriggerSampleRate": "100000", "520-Description": "DA device that is used as a shutter", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "405 Shutter-Sequenceable": "1", "405 Shutter-SupportsTriggering": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-TriggerSampleRate": "100000", "640 Shutter-MaxVolts": "5.0000", "555 AO-MaxVolts": "5.0000", "520 AO-TriggerSequenceLength": "1000", "COM3-BaudRate": "9600", "COM9-BaudRate": "9600", "Vincent-D3-Channel #": "Ch. #1", "PRIME-PMode": "Normal", "OpticalPath-Label": "Left(100)", "BitDepth": 16, "555 AO-TriggerSequenceLength": "1000", "445 Shutter-Description": "NI DAC", "405 AO-Sequenceable": "1", "PRIME-AcquisitionMethod": "Callbacks", "555 AO-Name": "AnalogIO", "PRIME-ShutterMode": "Pre-Exposure", "COM3-Handshaking": "Off", "445 AO-SupportsTriggering": "0", "ROI": "0-0-1200-1200", "COM9-Verbose": "1", "PRIME-Color": "OFF", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "PRIME-ShutterOpenDelay": "0", "COM1-Parity": "None", "Slice": 6, "SlicePosition": 10, "COM9-DelayBetweenCharsMs": "0.0000", "555-Name": "DA Shutter", "PIEZO-LudlSingleAxisName": "B", "XLIGHT Emission Wheel-Label": "585/11(T)", "640 Shutter-Name": "AnalogIO", "640 AO-Sequenceable": "1", "520 Shutter-Demo": "No", "528 Shutter-Demo": "No", "470-Name": "DA Shutter", "520-DA Device": "520 Shutter", "XYStage-Description": "Ludl XY stage driver adapter", "445 Shutter-MinVolts": "0.0000", "528 AO-TriggerSampleRate": "100000", "405 AO-MinVolts": "0.0000", "405 Shutter-TriggerSequenceLength": "1000", "Height": 1200, "555 AO-Block voltage": "false", "640 Shutter-Volts": "0.0000", "PixelType": "GRAY16", "445 Shutter-Block voltage": "false", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "520 Shutter-TriggerSequenceLength": "1000", "COM9-Name": "COM9", "Core-SLM": "", "555 AO-Volts": "4.5000", "555 Shutter-Block voltage": "false", "XYStage-Speed": "1800.0000", "520 AO-MaxVolts": "5.0000", "528 Shutter-SupportsTriggering": "0", "COM1-Description": "Serial port driver (boost:asio)", "PRIME-MetadataEnabled": "No", "520 Shutter-MinVolts": "0.0000", "470 Shutter-Sequenceable": "1", "PRIME-PreampDelay": "0", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-MinVolts": "0.0000", "ChannelIndex": 1, "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Core-AutoShutter": "1", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "Exposure-ms": 160, "PRIME-PP 2 ENABLED": "Yes", "640 Shutter-TriggerSampleRate": "100000", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "470 Shutter-IOChannel": "Dev1/ao10", "405 AO-Demo": "No", "PixelSizeUm": 1.1, "470 AO-Demo": "No", "470 Shutter-MaxVolts": "5.0000", "Vincent-D3-Name": "Vincent-D3", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FileName": "img_000000000_Il-T_006.tif", "LudlController-Reset": "Operate", "PRIME-Exposure": "160.00", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "445-Name": "DA Shutter", "555 Shutter-Name": "AnalogIO", "520 Shutter-TriggerSampleRate": "100000", "445 Shutter-IOChannel": "Dev1/ao9", "PRIME-TransposeCorrection": "0", "XYStage-StepSize": "0.1000", "445-Description": "DA device that is used as a shutter", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 AO-IOChannel": "Dev1/ao7", "520 Shutter-Name": "AnalogIO", "COM3-DataBits": "8", "640 AO-Volts": "0.0000", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "555 Shutter-IOChannel": "Dev1/ao13", "WaitInterval": null, "520 Shutter-Sequenceable": "1", "YPositionUm": 20690.300000000003, "445-State": "0", "445-DA Device": "445 Shutter", "PRIME-CircularBufferAutoSize": "ON", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "555 AO-TriggerSampleRate": "100000", "445 Shutter-Volts": "0.0000", "XLIGHT Hub-Port": "COM3", "Core-AutoFocus": "", "COM4-Handshaking": "Off", "528 Shutter-MinVolts": "0.0000", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "445 Shutter-Name": "AnalogIO", "528 Shutter-Sequenceable": "1", "XYStage-TransposeMirrorX": "0", "XYStage-TransposeMirrorY": "0", "PRIME-ReadoutRate": "100MHz 16bit", "XYStage-Name": "Stage", "640 AO-SupportsTriggering": "0", "640-Name": "DA Shutter", "640 Shutter-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "640 AO-Block voltage": "false", "445 Shutter-Sequenceable": "1", "470 AO-MaxVolts": "5.0000", "COM4-BaudRate": "9600", "470-Description": "DA device that is used as a shutter", "520 Shutter-Description": "NI DAC", "PRIME-CCDTemperature": "-14.9600", "Time": "2019-12-17 21:58:49 -0500", "640 AO-TriggerSequenceLength": "1000", "470 AO-Name": "AnalogIO", "XLIGHT Disk slider-Label": "Disk Pos 40um", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "Binning": "1x1", "TE2000-ModelType": "1", "COM3-AnswerTimeout": "500.0000", "555 Shutter-SupportsTriggering": "0", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "TE2000-Port": "COM4", "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Description": "NI DAC", "COM4-AnswerTimeout": "500.0000", "COM1-DelayBetweenCharsMs": "0.0000", "FilterBlock-Label": "Confocal", "445 AO-Name": "AnalogIO", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-Timing-ReadoutTimeNs": "24672000.0000", "528 AO-Demo": "No", "520 AO-Sequenceable": "1", "555-Description": "DA device that is used as a shutter", "405-DA Device": "405 Shutter", "405 AO-TriggerSequenceLength": "1000", "640 AO-MinVolts": "0.0000", "640 Shutter-Block voltage": "false", "FrameIndex": 0, "PRIME-Port": "CMOS", "555 AO-MinVolts": "0.0000", "COM3-Description": "Serial port driver (boost:asio)", "528 AO-Description": "NI DAC", "555 AO-IOChannel": "Dev1/ao6", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "555 AO-TriggerInputLine": "/Dev1/PFI0", "PRIME-ActualInterval-ms": "374.3700", "LudlController-Port": "COM9", "445 Shutter-Demo": "No", "PRIME-Trigger-Expose Out-Mux": "1", "SliceIndex": 6, "PRIME-CCDTemperatureSetPoint": "-15.0000", "405 Shutter-MinVolts": "0.0000", "PRIME-ExposeOutMode": "First Row", "AxisPositions": null, "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Volts": "5.0000", "528-Name": "DA Shutter", "555-State": "1", "XLIGHT Dichroic-Label": "QUAD", "445 AO-Sequenceable": "1", "405-Name": "DA Shutter", "405 AO-IOChannel": "Dev1/ao0", "Core-Camera": "PRIME", "Focus-Name": "Focus", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "405 Shutter-IOChannel": "Dev1/ao8", "640 AO-Demo": "No", "640 Shutter-SupportsTriggering": "0", "445 AO-Demo": "No", "555-DA Device": "555 Shutter", "470 Shutter-Description": "NI DAC", "528 AO-Volts": "0.0000", "COM1-DataBits": "8", "Vincent-D3-Address": "x", "520 AO-SupportsTriggering": "0", "405-Description": "DA device that is used as a shutter", "640 Shutter-MinVolts": "0.0000", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "405 AO-Name": "AnalogIO", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "640 AO-TriggerInputLine": "/Dev1/PFI0", "405 AO-TriggerInputLine": "/Dev1/PFI0", "640-Description": "DA device that is used as a shutter", "445 Shutter-TriggerSampleRate": "100000", "470 Shutter-TriggerSequenceLength": "1000", "COM1-StopBits": "1", "520 Shutter-SupportsTriggering": "0", "405-State": "0", "Core-Initialize": "1", "XLIGHT Emission Wheel-State": "7", "520 AO-Description": "NI DAC", "528 AO-TriggerInputLine": "/Dev1/PFI0", "445 Shutter-MaxVolts": "5.0000", "COM9-Handshaking": "Off", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "XLIGHT Spin motor-Label": "On (spinning)", "PRIME-PP 5 ENABLED": "No", "UUID": "cf244d3e-9cda-415c-a046-bdca2e44c7e7", "PRIME-TriggerMode": "Internal Trigger", "COM1-Handshaking": "Off", "PRIME-Timing-PostTriggerDelayNs": "49344000.0000", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "Focus-Position": "16479.8000", "528 Shutter-Block voltage": "false", "Channel": "Il-T", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "555 Shutter-Demo": "No", "405 Shutter-Demo": "No", "528 AO-Name": "AnalogIO", "520-State": "0", "Core-Galvo": "", "405 Shutter-TriggerSampleRate": "100000", "Focus-FocusStepSize_(nm)": "100", "PRIME-PixelType": "16bit", "COM4-DataBits": "8", "470 AO-IOChannel": "Dev1/ao2", "520 AO-MinVolts": "0.0000", "COM9-Parity": "None", "PRIME-CircularBufferEnabled": "ON", "PRIME-PP 6 Reset": "No", "640 AO-Description": "NI DAC", "Source": "PRIME", "445 AO-MinVolts": "0.0000", "PIEZO-Name": "Stage", "445 AO-Description": "NI DAC", "PRIME-CircularBufferFrameCount": "46", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "520 AO-Volts": "0.0000", "528 Shutter-TriggerSampleRate": "100000", "XPositionUm": 62321, "OpticalPath-State": "4", "PRIME-PP 3 THRESHOLD": "80", "PRIME-PVCAM Version": "3.7.1", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Gain": "1-HDR", "640 Shutter-TriggerSequenceLength": "1000", "528 AO-SupportsTriggering": "0", "445 AO-TriggerInputLine": "/Dev1/PFI0", "OpticalPath-Name": "OpticalPath", "PRIME-PP 4 ENABLED": "Yes", "PRIME-PVCAM Adapter Version": "1.3.29", "COM3-Name": "COM3", "405 AO-MaxVolts": "5.0000", "520 Shutter-Block voltage": "false", "PRIME-Timing-ClearingTimeNs": "49344000.0000", "Core-Focus": "PIEZO", "528 AO-Block voltage": "false", "TE2000-Description": "Nikon TE2000 hub", "528 AO-MinVolts": "0.0000", "COM4-Parity": "None", "Summary": { "Slices": 11, "ChContrastMin": [ 0, 0, 0, 0, 0, 0 ], "PixelAspect": 1, "SlicesFirst": true, "Interval_ms": 0, "Time": "2019-12-17 21:58:39 -0500", "Prefix": "seq_3", "InitialPositionList": [ { "Label": "Pos0", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.435], "Focus": [16480.2], "XYStage": [ 69822.40000000001, 20100.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos1", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.425], "Focus": [16480.3], "XYStage": [ 69004, 20101 ] }, "GridColumnIndex": 0 }, { "Label": "Pos2", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.44], "Focus": [16480.1], "XYStage": [ 63119.5, 20690.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos3", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.405], "Focus": [16480.2], "XYStage": [ 62321, 20690.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos4", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.415], "Focus": [16480], "XYStage": [ 56604.100000000006, 20688.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos5", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.42000000000002], "Focus": [16480.1], "XYStage": [ 56034.200000000004, 20688.800000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos6", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.455], "Focus": [16480], "XYStage": [ 50889.3, 20496.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos7", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.43], "Focus": [16480.2], "XYStage": [ 50101.600000000006, 20496.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos8", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.67000000000002], "Focus": [16479.9], "XYStage": [ 44818.600000000006, 20496.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos9", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.67000000000002], "Focus": [16480], "XYStage": [ 43897.9, 20436.800000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos10", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.675], "Focus": [16479.9], "XYStage": [ 39670.600000000006, 20664.9 ] }, "GridColumnIndex": 0 }, { "Label": "Pos11", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.69], "Focus": [16479.9], "XYStage": [ 38734, 20664.600000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos12", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.695], "Focus": [16480.1], "XYStage": [ 34037.200000000004, 20664.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos13", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.685], "Focus": [16480.1], "XYStage": [ 33152, 20664.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos14", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.69], "Focus": [16480.1], "XYStage": [ 28122.800000000003, 20664.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos15", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.675], "Focus": [16480], "XYStage": [ 27422.9, 20664.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos16", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.64000000000001], "Focus": [16480], "XYStage": [ 23005.2, 20664.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos17", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.69], "Focus": [16479.9], "XYStage": [ 21820.600000000002, 20409.600000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos18", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.41], "Focus": [16480.1], "XYStage": [ 73697.6, -13222.800000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos19", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.455], "Focus": [16480.1], "XYStage": [ 72765.8, -13223 ] }, "GridColumnIndex": 0 }, { "Label": "Pos20", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.4], "Focus": [16480.2], "XYStage": [ 67445.5, -12303.800000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos21", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.415], "Focus": [16480.2], "XYStage": [ 66613.3, -12303.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos22", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.415], "Focus": [16480.1], "XYStage": [ 61819.4, -11936.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos23", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.405], "Focus": [16480.2], "XYStage": [ 61325.700000000004, -11937.1 ] }, "GridColumnIndex": 0 }, { "Label": "Pos24", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.46], "Focus": [16480.2], "XYStage": [ 55377, -10974.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos25", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.445], "Focus": [16480.1], "XYStage": [ 54827, -11214.800000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos26", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.435], "Focus": [16480.2], "XYStage": [ 49937.100000000006, -10919.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos27", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.425], "Focus": [16480.1], "XYStage": [ 48764, -10919.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos28", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.42000000000002], "Focus": [16480.2], "XYStage": [ 43978.4, -10920.400000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos29", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.435], "Focus": [16480.2], "XYStage": [ 43106.3, -10920.400000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos30", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.405], "Focus": [16480.3], "XYStage": [ 38212.4, -10920.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos31", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.445], "Focus": [16480.2], "XYStage": [ 37254.9, -10920.400000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos32", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.44], "Focus": [16480.3], "XYStage": [ 32472.800000000003, -11294.400000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos33", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [166.95000000000002], "Focus": [16480.2], "XYStage": [ 31420.7, -11294.400000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos34", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [166.95000000000002], "Focus": [16480.2], "XYStage": [ 26881.5, -11640 ] }, "GridColumnIndex": 0 }, { "Label": "Pos35", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [166.98], "Focus": [16480.2], "XYStage": [ 25876.100000000002, -11641.1 ] }, "GridColumnIndex": 0 } ], "z-step_um": 10, "GridRow": 0, "BitDepth": 16, "Depth": 2, "Channels": 6, "Source": "Micro-Manager", "ChColors": [ -16711681, -256, -65281, -1, -1, -16776961 ], "MetadataVersion": 10, "KeepShutterOpenChannels": true, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536, 65536 ], "CustomIntervals_ms": [], "Height": 1200, "MicroManagerVersion": "1.4.23 20180219", "GridColumn": 0, "UUID": "8c8f9d20-b128-4577-9f91-89e70f22d319", "PixelType": "GRAY16", "Width": 1200, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\Yan\\20191217-YC113-S5S6", "Frames": 1, "Comment": "", "UserName": "Nikon", "PixelSize_um": 1.1, "ROI": [ 0, 0, 1200, 1200 ], "Date": "2019-12-17", "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC", "P-DAPI-high" ], "KeepShutterOpenSlices": true, "IJType": 1, "ComputerName": "ZadorConfocal2", "Positions": 36, "PositionIndex": 3, "CameraTimeout": 20000, "TimeFirst": false }, "640 AO-Name": "AnalogIO", "Vincent-D3-Command": "Close" }, "FrameKey-0-1-7": { "COM4-StopBits": "1", "528 AO-MaxVolts": "5.0000", "XLIGHT Excitation Wheel-State": "2", "470 Shutter-Name": "AnalogIO", "Core-Shutter": "555", "555 AO-Demo": "No", "XLIGHT Dichroic-State": "2", "XYStage-StepSize-Y": "0.1000", "ZPositionUm": 197.405, "XYStage-StepSize-X": "0.1000", "PRIME-FirmwareVersion": "20.28", "PRIME-ClearCycles": "2", "520-Name": "DA Shutter", "405 Shutter-Name": "AnalogIO", "PIEZO-StepSize": "0.0050", "PRIME-ClearMode": "Pre-Exposure", "470 AO-MinVolts": "0.0000", "405 AO-SupportsTriggering": "1", "CameraChannelIndex": 0, "XYStage-StartSpeed": "500.0000", "COM9-StopBits": "2", "405 AO-Description": "NI DAC", "COM4-DelayBetweenCharsMs": "0.0000", "445 AO-Block voltage": "false", "520 AO-TriggerInputLine": "/Dev1/PFI0", "555 AO-Description": "NI DAC", "520 AO-TriggerSampleRate": "100000", "COM4-Verbose": "1", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM4-Name": "COM4", "405 Shutter-Volts": "0.0000", "Vincent-D3-Port": "COM1", "PRIME-PP 4 MIN ADU AFFECTED": "200", "470 Shutter-MinVolts": "0.0000", "PRIME-PP 3 ENABLED": "Yes", "470 AO-Description": "NI DAC", "PRIME-Description": "PVCAM API device adapter", "520 Shutter-MaxVolts": "5.0000", "520 Shutter-Volts": "0.0000", "528 AO-Sequenceable": "1", "PRIME-Binning": "1x1", "555 Shutter-MaxVolts": "5.0000", "528 Shutter-TriggerSequenceLength": "1000", "405 Shutter-Block voltage": "false", "XLIGHT Spin motor-State": "1", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "Vincent-D3-Time to open (ms)": "35.0000", "Vincent-D3-Time to close (ms)": "35.0000", "445 AO-TriggerSampleRate": "100000", "640 Shutter-IOChannel": "Dev1/ao14", "555 AO-Sequenceable": "1", "520 AO-Name": "AnalogIO", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "470 AO-Block voltage": "false", "COM1-Verbose": "1", "COM9-AnswerTimeout": "10000.0000", "528 Shutter-Name": "AnalogIO", "PRIME-Trigger Timeout (secs)": "10", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "NextFrame": 0, "470 AO-TriggerSequenceLength": "1000", "PRIME-TransposeXY": "0", "445 AO-TriggerSequenceLength": "1000", "COM1-Name": "COM1", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "COM9-DataBits": "8", "COM1-AnswerTimeout": "500.0000", "Camera": "", "TE2000-Version": "V2.50-V2.50-V2.10", "528 Shutter-Volts": "0.0000", "XLIGHT Hub-Name": "XLIGHT Hub", "PIEZO-Description": "Ludl stage driver adapter", "PositionName": "Pos3", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSampleRate": "100000", "PRIME-FanSpeedSetpoint": "High", "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "405 AO-TriggerSampleRate": "100000", "470 Shutter-Block voltage": "false", "470-State": "0", "COM3-Parity": "None", "405 AO-Volts": "0.0000", "PRIME-TransposeMirrorY": "0", "PRIME-ChipName": "GS144BSI", "470 AO-Volts": "0.0000", "PRIME-TransposeMirrorX": "0", "445 Shutter-SupportsTriggering": "0", "470 Shutter-SupportsTriggering": "0", "XLIGHT Hub-Description": "XLIGHT hub", "PRIME-PreampOffLimit": "10000", "470 AO-Sequenceable": "1", "528 Shutter-MaxVolts": "5.0000", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "Core-XYStage": "XYStage", "470 AO-TriggerSampleRate": "100000", "PRIME-Timing-PreTriggerDelayNs": "24672000.0000", "528-DA Device": "528 Shutter", "Focus-Description": "Nikon TE2000 focus adapter", "FilterBlock-State": "5", "520 AO-Demo": "No", "COM3-DelayBetweenCharsMs": "0.0000", "470 Shutter-Volts": "0.0000", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "PRIME-PP 2 THRESHOLD": "125", "PRIME-PP 4 THRESHOLD": "75", "XYStage-Acceleration": "4.0000", "470 Shutter-Demo": "No", "PRIME-Name": "PMPCIECam00", "640-DA Device": "640 Shutter", "405 AO-Block voltage": "false", "ElapsedTime-ms": 142099, "PRIME-Offset": "100", "445 AO-IOChannel": "Dev1/ao1", "640 AO-MaxVolts": "5.0000", "PositionIndex": 3, "PRIME-PP 1 ENABLED": "Yes", "COM1-BaudRate": "9600", "640-State": "0", "COM3-Verbose": "1", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "LudlController-TransmissionDelay": "0", "PRIME-FullWellCapacity": "62000", "PRIME-SMARTStreamingEnabled": "No", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "640 Shutter-Description": "NI DAC", "528-Description": "DA device that is used as a shutter", "COM4-Description": "Serial port driver (boost:asio)", "405 Shutter-Description": "NI DAC", "TE2000-Name": "TE2000", "528 Shutter-Description": "NI DAC", "COM9-Description": "Serial port driver (boost:asio)", "LudlController-Name": "LudlController", "445 Shutter-TriggerSequenceLength": "1000", "PRIME-SerialNumber": "A18B203008", "445 AO-Volts": "0.0000", "PRIME-X-dimension": "1200", "PRIME-ShutterCloseDelay": "0", "XLIGHT Disk slider-State": "2", "Position": "Default", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 AO-IOChannel": "Dev1/ao5", "520 AO-IOChannel": "Dev1/ao4", "528 AO-TriggerSequenceLength": "1000", "405 Shutter-MaxVolts": "5.0000", "470-DA Device": "470 Shutter", "PRIME-FTCapable": "No", "Core-ChannelGroup": "Channels", "555 AO-SupportsTriggering": "0", "PRIME-Y-dimension": "1200", "Frame": 0, "555 Shutter-Sequenceable": "1", "PRIME-Timing-ExposureTimeNs": "9992000.0000", "Core-ImageProcessor": "", "528-State": "0", "520 AO-Block voltage": "false", "XLIGHT Excitation Wheel-Label": "QUAD", "640 Shutter-Demo": "No", "COM3-StopBits": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "520 Shutter-IOChannel": "Dev1/ao11", "Width": 1200, "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-SupportsTriggering": "0", "640 AO-TriggerSampleRate": "100000", "520-Description": "DA device that is used as a shutter", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "405 Shutter-Sequenceable": "1", "405 Shutter-SupportsTriggering": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-TriggerSampleRate": "100000", "640 Shutter-MaxVolts": "5.0000", "555 AO-MaxVolts": "5.0000", "520 AO-TriggerSequenceLength": "1000", "COM3-BaudRate": "9600", "COM9-BaudRate": "9600", "Vincent-D3-Channel #": "Ch. #1", "PRIME-PMode": "Normal", "OpticalPath-Label": "Left(100)", "BitDepth": 16, "555 AO-TriggerSequenceLength": "1000", "445 Shutter-Description": "NI DAC", "405 AO-Sequenceable": "1", "PRIME-AcquisitionMethod": "Callbacks", "555 AO-Name": "AnalogIO", "PRIME-ShutterMode": "Pre-Exposure", "COM3-Handshaking": "Off", "445 AO-SupportsTriggering": "0", "ROI": "0-0-1200-1200", "COM9-Verbose": "1", "PRIME-Color": "OFF", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "PRIME-ShutterOpenDelay": "0", "COM1-Parity": "None", "Slice": 7, "SlicePosition": 20, "COM9-DelayBetweenCharsMs": "0.0000", "555-Name": "DA Shutter", "PIEZO-LudlSingleAxisName": "B", "XLIGHT Emission Wheel-Label": "585/11(T)", "640 Shutter-Name": "AnalogIO", "640 AO-Sequenceable": "1", "520 Shutter-Demo": "No", "528 Shutter-Demo": "No", "470-Name": "DA Shutter", "520-DA Device": "520 Shutter", "XYStage-Description": "Ludl XY stage driver adapter", "445 Shutter-MinVolts": "0.0000", "528 AO-TriggerSampleRate": "100000", "405 AO-MinVolts": "0.0000", "405 Shutter-TriggerSequenceLength": "1000", "Height": 1200, "555 AO-Block voltage": "false", "640 Shutter-Volts": "0.0000", "PixelType": "GRAY16", "445 Shutter-Block voltage": "false", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "520 Shutter-TriggerSequenceLength": "1000", "COM9-Name": "COM9", "Core-SLM": "", "555 AO-Volts": "4.5000", "555 Shutter-Block voltage": "false", "XYStage-Speed": "1800.0000", "520 AO-MaxVolts": "5.0000", "528 Shutter-SupportsTriggering": "0", "COM1-Description": "Serial port driver (boost:asio)", "PRIME-MetadataEnabled": "No", "520 Shutter-MinVolts": "0.0000", "470 Shutter-Sequenceable": "1", "PRIME-PreampDelay": "0", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-MinVolts": "0.0000", "ChannelIndex": 1, "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Core-AutoShutter": "1", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "Exposure-ms": 160, "PRIME-PP 2 ENABLED": "Yes", "640 Shutter-TriggerSampleRate": "100000", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "470 Shutter-IOChannel": "Dev1/ao10", "405 AO-Demo": "No", "PixelSizeUm": 1.1, "470 AO-Demo": "No", "470 Shutter-MaxVolts": "5.0000", "Vincent-D3-Name": "Vincent-D3", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FileName": "img_000000000_Il-T_007.tif", "LudlController-Reset": "Operate", "PRIME-Exposure": "160.00", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "445-Name": "DA Shutter", "555 Shutter-Name": "AnalogIO", "520 Shutter-TriggerSampleRate": "100000", "445 Shutter-IOChannel": "Dev1/ao9", "PRIME-TransposeCorrection": "0", "XYStage-StepSize": "0.1000", "445-Description": "DA device that is used as a shutter", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 AO-IOChannel": "Dev1/ao7", "520 Shutter-Name": "AnalogIO", "COM3-DataBits": "8", "640 AO-Volts": "0.0000", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "555 Shutter-IOChannel": "Dev1/ao13", "WaitInterval": null, "520 Shutter-Sequenceable": "1", "YPositionUm": 20690.300000000003, "445-State": "0", "445-DA Device": "445 Shutter", "PRIME-CircularBufferAutoSize": "ON", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "555 AO-TriggerSampleRate": "100000", "445 Shutter-Volts": "0.0000", "XLIGHT Hub-Port": "COM3", "Core-AutoFocus": "", "COM4-Handshaking": "Off", "528 Shutter-MinVolts": "0.0000", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "445 Shutter-Name": "AnalogIO", "528 Shutter-Sequenceable": "1", "XYStage-TransposeMirrorX": "0", "XYStage-TransposeMirrorY": "0", "PRIME-ReadoutRate": "100MHz 16bit", "XYStage-Name": "Stage", "640 AO-SupportsTriggering": "0", "640-Name": "DA Shutter", "640 Shutter-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "640 AO-Block voltage": "false", "445 Shutter-Sequenceable": "1", "470 AO-MaxVolts": "5.0000", "COM4-BaudRate": "9600", "470-Description": "DA device that is used as a shutter", "520 Shutter-Description": "NI DAC", "PRIME-CCDTemperature": "-14.9600", "Time": "2019-12-17 21:58:49 -0500", "640 AO-TriggerSequenceLength": "1000", "470 AO-Name": "AnalogIO", "XLIGHT Disk slider-Label": "Disk Pos 40um", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "Binning": "1x1", "TE2000-ModelType": "1", "COM3-AnswerTimeout": "500.0000", "555 Shutter-SupportsTriggering": "0", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "TE2000-Port": "COM4", "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Description": "NI DAC", "COM4-AnswerTimeout": "500.0000", "COM1-DelayBetweenCharsMs": "0.0000", "FilterBlock-Label": "Confocal", "445 AO-Name": "AnalogIO", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-Timing-ReadoutTimeNs": "24672000.0000", "528 AO-Demo": "No", "520 AO-Sequenceable": "1", "555-Description": "DA device that is used as a shutter", "405-DA Device": "405 Shutter", "405 AO-TriggerSequenceLength": "1000", "640 AO-MinVolts": "0.0000", "640 Shutter-Block voltage": "false", "FrameIndex": 0, "PRIME-Port": "CMOS", "555 AO-MinVolts": "0.0000", "COM3-Description": "Serial port driver (boost:asio)", "528 AO-Description": "NI DAC", "555 AO-IOChannel": "Dev1/ao6", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "555 AO-TriggerInputLine": "/Dev1/PFI0", "PRIME-ActualInterval-ms": "374.3700", "LudlController-Port": "COM9", "445 Shutter-Demo": "No", "PRIME-Trigger-Expose Out-Mux": "1", "SliceIndex": 7, "PRIME-CCDTemperatureSetPoint": "-15.0000", "405 Shutter-MinVolts": "0.0000", "PRIME-ExposeOutMode": "First Row", "AxisPositions": null, "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Volts": "5.0000", "528-Name": "DA Shutter", "555-State": "1", "XLIGHT Dichroic-Label": "QUAD", "445 AO-Sequenceable": "1", "405-Name": "DA Shutter", "405 AO-IOChannel": "Dev1/ao0", "Core-Camera": "PRIME", "Focus-Name": "Focus", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "405 Shutter-IOChannel": "Dev1/ao8", "640 AO-Demo": "No", "640 Shutter-SupportsTriggering": "0", "445 AO-Demo": "No", "555-DA Device": "555 Shutter", "470 Shutter-Description": "NI DAC", "528 AO-Volts": "0.0000", "COM1-DataBits": "8", "Vincent-D3-Address": "x", "520 AO-SupportsTriggering": "0", "405-Description": "DA device that is used as a shutter", "640 Shutter-MinVolts": "0.0000", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "405 AO-Name": "AnalogIO", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "640 AO-TriggerInputLine": "/Dev1/PFI0", "405 AO-TriggerInputLine": "/Dev1/PFI0", "640-Description": "DA device that is used as a shutter", "445 Shutter-TriggerSampleRate": "100000", "470 Shutter-TriggerSequenceLength": "1000", "COM1-StopBits": "1", "520 Shutter-SupportsTriggering": "0", "405-State": "0", "Core-Initialize": "1", "XLIGHT Emission Wheel-State": "7", "520 AO-Description": "NI DAC", "528 AO-TriggerInputLine": "/Dev1/PFI0", "445 Shutter-MaxVolts": "5.0000", "COM9-Handshaking": "Off", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "XLIGHT Spin motor-Label": "On (spinning)", "PRIME-PP 5 ENABLED": "No", "UUID": "c29a2749-4ca5-4a27-968c-bc078b815435", "PRIME-TriggerMode": "Internal Trigger", "COM1-Handshaking": "Off", "PRIME-Timing-PostTriggerDelayNs": "49344000.0000", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "Focus-Position": "16479.8000", "528 Shutter-Block voltage": "false", "Channel": "Il-T", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "555 Shutter-Demo": "No", "405 Shutter-Demo": "No", "528 AO-Name": "AnalogIO", "520-State": "0", "Core-Galvo": "", "405 Shutter-TriggerSampleRate": "100000", "Focus-FocusStepSize_(nm)": "100", "PRIME-PixelType": "16bit", "COM4-DataBits": "8", "470 AO-IOChannel": "Dev1/ao2", "520 AO-MinVolts": "0.0000", "COM9-Parity": "None", "PRIME-CircularBufferEnabled": "ON", "PRIME-PP 6 Reset": "No", "640 AO-Description": "NI DAC", "Source": "PRIME", "445 AO-MinVolts": "0.0000", "PIEZO-Name": "Stage", "445 AO-Description": "NI DAC", "PRIME-CircularBufferFrameCount": "46", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "520 AO-Volts": "0.0000", "528 Shutter-TriggerSampleRate": "100000", "XPositionUm": 62321, "OpticalPath-State": "4", "PRIME-PP 3 THRESHOLD": "80", "PRIME-PVCAM Version": "3.7.1", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Gain": "1-HDR", "640 Shutter-TriggerSequenceLength": "1000", "528 AO-SupportsTriggering": "0", "445 AO-TriggerInputLine": "/Dev1/PFI0", "OpticalPath-Name": "OpticalPath", "PRIME-PP 4 ENABLED": "Yes", "PRIME-PVCAM Adapter Version": "1.3.29", "COM3-Name": "COM3", "405 AO-MaxVolts": "5.0000", "520 Shutter-Block voltage": "false", "PRIME-Timing-ClearingTimeNs": "49344000.0000", "Core-Focus": "PIEZO", "528 AO-Block voltage": "false", "TE2000-Description": "Nikon TE2000 hub", "528 AO-MinVolts": "0.0000", "COM4-Parity": "None", "Summary": { "Slices": 11, "ChContrastMin": [ 0, 0, 0, 0, 0, 0 ], "PixelAspect": 1, "SlicesFirst": true, "Interval_ms": 0, "Time": "2019-12-17 21:58:39 -0500", "Prefix": "seq_3", "InitialPositionList": [ { "Label": "Pos0", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.435], "Focus": [16480.2], "XYStage": [ 69822.40000000001, 20100.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos1", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.425], "Focus": [16480.3], "XYStage": [ 69004, 20101 ] }, "GridColumnIndex": 0 }, { "Label": "Pos2", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.44], "Focus": [16480.1], "XYStage": [ 63119.5, 20690.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos3", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.405], "Focus": [16480.2], "XYStage": [ 62321, 20690.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos4", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.415], "Focus": [16480], "XYStage": [ 56604.100000000006, 20688.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos5", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.42000000000002], "Focus": [16480.1], "XYStage": [ 56034.200000000004, 20688.800000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos6", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.455], "Focus": [16480], "XYStage": [ 50889.3, 20496.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos7", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.43], "Focus": [16480.2], "XYStage": [ 50101.600000000006, 20496.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos8", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.67000000000002], "Focus": [16479.9], "XYStage": [ 44818.600000000006, 20496.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos9", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.67000000000002], "Focus": [16480], "XYStage": [ 43897.9, 20436.800000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos10", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.675], "Focus": [16479.9], "XYStage": [ 39670.600000000006, 20664.9 ] }, "GridColumnIndex": 0 }, { "Label": "Pos11", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.69], "Focus": [16479.9], "XYStage": [ 38734, 20664.600000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos12", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.695], "Focus": [16480.1], "XYStage": [ 34037.200000000004, 20664.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos13", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.685], "Focus": [16480.1], "XYStage": [ 33152, 20664.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos14", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.69], "Focus": [16480.1], "XYStage": [ 28122.800000000003, 20664.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos15", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.675], "Focus": [16480], "XYStage": [ 27422.9, 20664.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos16", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.64000000000001], "Focus": [16480], "XYStage": [ 23005.2, 20664.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos17", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.69], "Focus": [16479.9], "XYStage": [ 21820.600000000002, 20409.600000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos18", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.41], "Focus": [16480.1], "XYStage": [ 73697.6, -13222.800000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos19", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.455], "Focus": [16480.1], "XYStage": [ 72765.8, -13223 ] }, "GridColumnIndex": 0 }, { "Label": "Pos20", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.4], "Focus": [16480.2], "XYStage": [ 67445.5, -12303.800000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos21", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.415], "Focus": [16480.2], "XYStage": [ 66613.3, -12303.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos22", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.415], "Focus": [16480.1], "XYStage": [ 61819.4, -11936.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos23", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.405], "Focus": [16480.2], "XYStage": [ 61325.700000000004, -11937.1 ] }, "GridColumnIndex": 0 }, { "Label": "Pos24", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.46], "Focus": [16480.2], "XYStage": [ 55377, -10974.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos25", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.445], "Focus": [16480.1], "XYStage": [ 54827, -11214.800000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos26", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.435], "Focus": [16480.2], "XYStage": [ 49937.100000000006, -10919.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos27", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.425], "Focus": [16480.1], "XYStage": [ 48764, -10919.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos28", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.42000000000002], "Focus": [16480.2], "XYStage": [ 43978.4, -10920.400000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos29", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.435], "Focus": [16480.2], "XYStage": [ 43106.3, -10920.400000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos30", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.405], "Focus": [16480.3], "XYStage": [ 38212.4, -10920.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos31", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.445], "Focus": [16480.2], "XYStage": [ 37254.9, -10920.400000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos32", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.44], "Focus": [16480.3], "XYStage": [ 32472.800000000003, -11294.400000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos33", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [166.95000000000002], "Focus": [16480.2], "XYStage": [ 31420.7, -11294.400000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos34", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [166.95000000000002], "Focus": [16480.2], "XYStage": [ 26881.5, -11640 ] }, "GridColumnIndex": 0 }, { "Label": "Pos35", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [166.98], "Focus": [16480.2], "XYStage": [ 25876.100000000002, -11641.1 ] }, "GridColumnIndex": 0 } ], "z-step_um": 10, "GridRow": 0, "BitDepth": 16, "Depth": 2, "Channels": 6, "Source": "Micro-Manager", "ChColors": [ -16711681, -256, -65281, -1, -1, -16776961 ], "MetadataVersion": 10, "KeepShutterOpenChannels": true, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536, 65536 ], "CustomIntervals_ms": [], "Height": 1200, "MicroManagerVersion": "1.4.23 20180219", "GridColumn": 0, "UUID": "8c8f9d20-b128-4577-9f91-89e70f22d319", "PixelType": "GRAY16", "Width": 1200, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\Yan\\20191217-YC113-S5S6", "Frames": 1, "Comment": "", "UserName": "Nikon", "PixelSize_um": 1.1, "ROI": [ 0, 0, 1200, 1200 ], "Date": "2019-12-17", "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC", "P-DAPI-high" ], "KeepShutterOpenSlices": true, "IJType": 1, "ComputerName": "ZadorConfocal2", "Positions": 36, "PositionIndex": 3, "CameraTimeout": 20000, "TimeFirst": false }, "640 AO-Name": "AnalogIO", "Vincent-D3-Command": "Close" }, "FrameKey-0-1-8": { "COM4-StopBits": "1", "528 AO-MaxVolts": "5.0000", "XLIGHT Excitation Wheel-State": "2", "470 Shutter-Name": "AnalogIO", "Core-Shutter": "555", "555 AO-Demo": "No", "XLIGHT Dichroic-State": "2", "XYStage-StepSize-Y": "0.1000", "ZPositionUm": 207.405, "XYStage-StepSize-X": "0.1000", "PRIME-FirmwareVersion": "20.28", "PRIME-ClearCycles": "2", "520-Name": "DA Shutter", "405 Shutter-Name": "AnalogIO", "PIEZO-StepSize": "0.0050", "PRIME-ClearMode": "Pre-Exposure", "470 AO-MinVolts": "0.0000", "405 AO-SupportsTriggering": "1", "CameraChannelIndex": 0, "XYStage-StartSpeed": "500.0000", "COM9-StopBits": "2", "405 AO-Description": "NI DAC", "COM4-DelayBetweenCharsMs": "0.0000", "445 AO-Block voltage": "false", "520 AO-TriggerInputLine": "/Dev1/PFI0", "555 AO-Description": "NI DAC", "520 AO-TriggerSampleRate": "100000", "COM4-Verbose": "1", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM4-Name": "COM4", "405 Shutter-Volts": "0.0000", "Vincent-D3-Port": "COM1", "PRIME-PP 4 MIN ADU AFFECTED": "200", "470 Shutter-MinVolts": "0.0000", "PRIME-PP 3 ENABLED": "Yes", "470 AO-Description": "NI DAC", "PRIME-Description": "PVCAM API device adapter", "520 Shutter-MaxVolts": "5.0000", "520 Shutter-Volts": "0.0000", "528 AO-Sequenceable": "1", "PRIME-Binning": "1x1", "555 Shutter-MaxVolts": "5.0000", "528 Shutter-TriggerSequenceLength": "1000", "405 Shutter-Block voltage": "false", "XLIGHT Spin motor-State": "1", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "Vincent-D3-Time to open (ms)": "35.0000", "Vincent-D3-Time to close (ms)": "35.0000", "445 AO-TriggerSampleRate": "100000", "640 Shutter-IOChannel": "Dev1/ao14", "555 AO-Sequenceable": "1", "520 AO-Name": "AnalogIO", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "470 AO-Block voltage": "false", "COM1-Verbose": "1", "COM9-AnswerTimeout": "10000.0000", "528 Shutter-Name": "AnalogIO", "PRIME-Trigger Timeout (secs)": "10", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "NextFrame": 0, "470 AO-TriggerSequenceLength": "1000", "PRIME-TransposeXY": "0", "445 AO-TriggerSequenceLength": "1000", "COM1-Name": "COM1", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "COM9-DataBits": "8", "COM1-AnswerTimeout": "500.0000", "Camera": "", "TE2000-Version": "V2.50-V2.50-V2.10", "528 Shutter-Volts": "0.0000", "XLIGHT Hub-Name": "XLIGHT Hub", "PIEZO-Description": "Ludl stage driver adapter", "PositionName": "Pos3", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSampleRate": "100000", "PRIME-FanSpeedSetpoint": "High", "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "405 AO-TriggerSampleRate": "100000", "470 Shutter-Block voltage": "false", "470-State": "0", "COM3-Parity": "None", "405 AO-Volts": "0.0000", "PRIME-TransposeMirrorY": "0", "PRIME-ChipName": "GS144BSI", "470 AO-Volts": "0.0000", "PRIME-TransposeMirrorX": "0", "445 Shutter-SupportsTriggering": "0", "470 Shutter-SupportsTriggering": "0", "XLIGHT Hub-Description": "XLIGHT hub", "PRIME-PreampOffLimit": "10000", "470 AO-Sequenceable": "1", "528 Shutter-MaxVolts": "5.0000", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "Core-XYStage": "XYStage", "470 AO-TriggerSampleRate": "100000", "PRIME-Timing-PreTriggerDelayNs": "24672000.0000", "528-DA Device": "528 Shutter", "Focus-Description": "Nikon TE2000 focus adapter", "FilterBlock-State": "5", "520 AO-Demo": "No", "COM3-DelayBetweenCharsMs": "0.0000", "470 Shutter-Volts": "0.0000", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "PRIME-PP 2 THRESHOLD": "125", "PRIME-PP 4 THRESHOLD": "75", "XYStage-Acceleration": "4.0000", "470 Shutter-Demo": "No", "PRIME-Name": "PMPCIECam00", "640-DA Device": "640 Shutter", "405 AO-Block voltage": "false", "ElapsedTime-ms": 142505, "PRIME-Offset": "100", "445 AO-IOChannel": "Dev1/ao1", "640 AO-MaxVolts": "5.0000", "PositionIndex": 3, "PRIME-PP 1 ENABLED": "Yes", "COM1-BaudRate": "9600", "640-State": "0", "COM3-Verbose": "1", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "LudlController-TransmissionDelay": "0", "PRIME-FullWellCapacity": "62000", "PRIME-SMARTStreamingEnabled": "No", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "640 Shutter-Description": "NI DAC", "528-Description": "DA device that is used as a shutter", "COM4-Description": "Serial port driver (boost:asio)", "405 Shutter-Description": "NI DAC", "TE2000-Name": "TE2000", "528 Shutter-Description": "NI DAC", "COM9-Description": "Serial port driver (boost:asio)", "LudlController-Name": "LudlController", "445 Shutter-TriggerSequenceLength": "1000", "PRIME-SerialNumber": "A18B203008", "445 AO-Volts": "0.0000", "PRIME-X-dimension": "1200", "PRIME-ShutterCloseDelay": "0", "XLIGHT Disk slider-State": "2", "Position": "Default", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 AO-IOChannel": "Dev1/ao5", "520 AO-IOChannel": "Dev1/ao4", "528 AO-TriggerSequenceLength": "1000", "405 Shutter-MaxVolts": "5.0000", "470-DA Device": "470 Shutter", "PRIME-FTCapable": "No", "Core-ChannelGroup": "Channels", "555 AO-SupportsTriggering": "0", "PRIME-Y-dimension": "1200", "Frame": 0, "555 Shutter-Sequenceable": "1", "PRIME-Timing-ExposureTimeNs": "9992000.0000", "Core-ImageProcessor": "", "528-State": "0", "520 AO-Block voltage": "false", "XLIGHT Excitation Wheel-Label": "QUAD", "640 Shutter-Demo": "No", "COM3-StopBits": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "520 Shutter-IOChannel": "Dev1/ao11", "Width": 1200, "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-SupportsTriggering": "0", "640 AO-TriggerSampleRate": "100000", "520-Description": "DA device that is used as a shutter", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "405 Shutter-Sequenceable": "1", "405 Shutter-SupportsTriggering": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-TriggerSampleRate": "100000", "640 Shutter-MaxVolts": "5.0000", "555 AO-MaxVolts": "5.0000", "520 AO-TriggerSequenceLength": "1000", "COM3-BaudRate": "9600", "COM9-BaudRate": "9600", "Vincent-D3-Channel #": "Ch. #1", "PRIME-PMode": "Normal", "OpticalPath-Label": "Left(100)", "BitDepth": 16, "555 AO-TriggerSequenceLength": "1000", "445 Shutter-Description": "NI DAC", "405 AO-Sequenceable": "1", "PRIME-AcquisitionMethod": "Callbacks", "555 AO-Name": "AnalogIO", "PRIME-ShutterMode": "Pre-Exposure", "COM3-Handshaking": "Off", "445 AO-SupportsTriggering": "0", "ROI": "0-0-1200-1200", "COM9-Verbose": "1", "PRIME-Color": "OFF", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "PRIME-ShutterOpenDelay": "0", "COM1-Parity": "None", "Slice": 8, "SlicePosition": 30, "COM9-DelayBetweenCharsMs": "0.0000", "555-Name": "DA Shutter", "PIEZO-LudlSingleAxisName": "B", "XLIGHT Emission Wheel-Label": "585/11(T)", "640 Shutter-Name": "AnalogIO", "640 AO-Sequenceable": "1", "520 Shutter-Demo": "No", "528 Shutter-Demo": "No", "470-Name": "DA Shutter", "520-DA Device": "520 Shutter", "XYStage-Description": "Ludl XY stage driver adapter", "445 Shutter-MinVolts": "0.0000", "528 AO-TriggerSampleRate": "100000", "405 AO-MinVolts": "0.0000", "405 Shutter-TriggerSequenceLength": "1000", "Height": 1200, "555 AO-Block voltage": "false", "640 Shutter-Volts": "0.0000", "PixelType": "GRAY16", "445 Shutter-Block voltage": "false", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "520 Shutter-TriggerSequenceLength": "1000", "COM9-Name": "COM9", "Core-SLM": "", "555 AO-Volts": "4.5000", "555 Shutter-Block voltage": "false", "XYStage-Speed": "1800.0000", "520 AO-MaxVolts": "5.0000", "528 Shutter-SupportsTriggering": "0", "COM1-Description": "Serial port driver (boost:asio)", "PRIME-MetadataEnabled": "No", "520 Shutter-MinVolts": "0.0000", "470 Shutter-Sequenceable": "1", "PRIME-PreampDelay": "0", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-MinVolts": "0.0000", "ChannelIndex": 1, "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Core-AutoShutter": "1", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "Exposure-ms": 160, "PRIME-PP 2 ENABLED": "Yes", "640 Shutter-TriggerSampleRate": "100000", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "470 Shutter-IOChannel": "Dev1/ao10", "405 AO-Demo": "No", "PixelSizeUm": 1.1, "470 AO-Demo": "No", "470 Shutter-MaxVolts": "5.0000", "Vincent-D3-Name": "Vincent-D3", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FileName": "img_000000000_Il-T_008.tif", "LudlController-Reset": "Operate", "PRIME-Exposure": "160.00", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "445-Name": "DA Shutter", "555 Shutter-Name": "AnalogIO", "520 Shutter-TriggerSampleRate": "100000", "445 Shutter-IOChannel": "Dev1/ao9", "PRIME-TransposeCorrection": "0", "XYStage-StepSize": "0.1000", "445-Description": "DA device that is used as a shutter", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 AO-IOChannel": "Dev1/ao7", "520 Shutter-Name": "AnalogIO", "COM3-DataBits": "8", "640 AO-Volts": "0.0000", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "555 Shutter-IOChannel": "Dev1/ao13", "WaitInterval": null, "520 Shutter-Sequenceable": "1", "YPositionUm": 20690.300000000003, "445-State": "0", "445-DA Device": "445 Shutter", "PRIME-CircularBufferAutoSize": "ON", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "555 AO-TriggerSampleRate": "100000", "445 Shutter-Volts": "0.0000", "XLIGHT Hub-Port": "COM3", "Core-AutoFocus": "", "COM4-Handshaking": "Off", "528 Shutter-MinVolts": "0.0000", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "445 Shutter-Name": "AnalogIO", "528 Shutter-Sequenceable": "1", "XYStage-TransposeMirrorX": "0", "XYStage-TransposeMirrorY": "0", "PRIME-ReadoutRate": "100MHz 16bit", "XYStage-Name": "Stage", "640 AO-SupportsTriggering": "0", "640-Name": "DA Shutter", "640 Shutter-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "640 AO-Block voltage": "false", "445 Shutter-Sequenceable": "1", "470 AO-MaxVolts": "5.0000", "COM4-BaudRate": "9600", "470-Description": "DA device that is used as a shutter", "520 Shutter-Description": "NI DAC", "PRIME-CCDTemperature": "-14.9600", "Time": "2019-12-17 21:58:49 -0500", "640 AO-TriggerSequenceLength": "1000", "470 AO-Name": "AnalogIO", "XLIGHT Disk slider-Label": "Disk Pos 40um", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "Binning": "1x1", "TE2000-ModelType": "1", "COM3-AnswerTimeout": "500.0000", "555 Shutter-SupportsTriggering": "0", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "TE2000-Port": "COM4", "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Description": "NI DAC", "COM4-AnswerTimeout": "500.0000", "COM1-DelayBetweenCharsMs": "0.0000", "FilterBlock-Label": "Confocal", "445 AO-Name": "AnalogIO", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-Timing-ReadoutTimeNs": "24672000.0000", "528 AO-Demo": "No", "520 AO-Sequenceable": "1", "555-Description": "DA device that is used as a shutter", "405-DA Device": "405 Shutter", "405 AO-TriggerSequenceLength": "1000", "640 AO-MinVolts": "0.0000", "640 Shutter-Block voltage": "false", "FrameIndex": 0, "PRIME-Port": "CMOS", "555 AO-MinVolts": "0.0000", "COM3-Description": "Serial port driver (boost:asio)", "528 AO-Description": "NI DAC", "555 AO-IOChannel": "Dev1/ao6", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "555 AO-TriggerInputLine": "/Dev1/PFI0", "PRIME-ActualInterval-ms": "374.3700", "LudlController-Port": "COM9", "445 Shutter-Demo": "No", "PRIME-Trigger-Expose Out-Mux": "1", "SliceIndex": 8, "PRIME-CCDTemperatureSetPoint": "-15.0000", "405 Shutter-MinVolts": "0.0000", "PRIME-ExposeOutMode": "First Row", "AxisPositions": null, "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Volts": "5.0000", "528-Name": "DA Shutter", "555-State": "1", "XLIGHT Dichroic-Label": "QUAD", "445 AO-Sequenceable": "1", "405-Name": "DA Shutter", "405 AO-IOChannel": "Dev1/ao0", "Core-Camera": "PRIME", "Focus-Name": "Focus", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "405 Shutter-IOChannel": "Dev1/ao8", "640 AO-Demo": "No", "640 Shutter-SupportsTriggering": "0", "445 AO-Demo": "No", "555-DA Device": "555 Shutter", "470 Shutter-Description": "NI DAC", "528 AO-Volts": "0.0000", "COM1-DataBits": "8", "Vincent-D3-Address": "x", "520 AO-SupportsTriggering": "0", "405-Description": "DA device that is used as a shutter", "640 Shutter-MinVolts": "0.0000", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "405 AO-Name": "AnalogIO", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "640 AO-TriggerInputLine": "/Dev1/PFI0", "405 AO-TriggerInputLine": "/Dev1/PFI0", "640-Description": "DA device that is used as a shutter", "445 Shutter-TriggerSampleRate": "100000", "470 Shutter-TriggerSequenceLength": "1000", "COM1-StopBits": "1", "520 Shutter-SupportsTriggering": "0", "405-State": "0", "Core-Initialize": "1", "XLIGHT Emission Wheel-State": "7", "520 AO-Description": "NI DAC", "528 AO-TriggerInputLine": "/Dev1/PFI0", "445 Shutter-MaxVolts": "5.0000", "COM9-Handshaking": "Off", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "XLIGHT Spin motor-Label": "On (spinning)", "PRIME-PP 5 ENABLED": "No", "UUID": "1f67e31d-c130-4a43-898a-1d982ae97070", "PRIME-TriggerMode": "Internal Trigger", "COM1-Handshaking": "Off", "PRIME-Timing-PostTriggerDelayNs": "49344000.0000", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "Focus-Position": "16479.8000", "528 Shutter-Block voltage": "false", "Channel": "Il-T", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "555 Shutter-Demo": "No", "405 Shutter-Demo": "No", "528 AO-Name": "AnalogIO", "520-State": "0", "Core-Galvo": "", "405 Shutter-TriggerSampleRate": "100000", "Focus-FocusStepSize_(nm)": "100", "PRIME-PixelType": "16bit", "COM4-DataBits": "8", "470 AO-IOChannel": "Dev1/ao2", "520 AO-MinVolts": "0.0000", "COM9-Parity": "None", "PRIME-CircularBufferEnabled": "ON", "PRIME-PP 6 Reset": "No", "640 AO-Description": "NI DAC", "Source": "PRIME", "445 AO-MinVolts": "0.0000", "PIEZO-Name": "Stage", "445 AO-Description": "NI DAC", "PRIME-CircularBufferFrameCount": "46", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "520 AO-Volts": "0.0000", "528 Shutter-TriggerSampleRate": "100000", "XPositionUm": 62321, "OpticalPath-State": "4", "PRIME-PP 3 THRESHOLD": "80", "PRIME-PVCAM Version": "3.7.1", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Gain": "1-HDR", "640 Shutter-TriggerSequenceLength": "1000", "528 AO-SupportsTriggering": "0", "445 AO-TriggerInputLine": "/Dev1/PFI0", "OpticalPath-Name": "OpticalPath", "PRIME-PP 4 ENABLED": "Yes", "PRIME-PVCAM Adapter Version": "1.3.29", "COM3-Name": "COM3", "405 AO-MaxVolts": "5.0000", "520 Shutter-Block voltage": "false", "PRIME-Timing-ClearingTimeNs": "49344000.0000", "Core-Focus": "PIEZO", "528 AO-Block voltage": "false", "TE2000-Description": "Nikon TE2000 hub", "528 AO-MinVolts": "0.0000", "COM4-Parity": "None", "Summary": { "Slices": 11, "ChContrastMin": [ 0, 0, 0, 0, 0, 0 ], "PixelAspect": 1, "SlicesFirst": true, "Interval_ms": 0, "Time": "2019-12-17 21:58:39 -0500", "Prefix": "seq_3", "InitialPositionList": [ { "Label": "Pos0", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.435], "Focus": [16480.2], "XYStage": [ 69822.40000000001, 20100.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos1", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.425], "Focus": [16480.3], "XYStage": [ 69004, 20101 ] }, "GridColumnIndex": 0 }, { "Label": "Pos2", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.44], "Focus": [16480.1], "XYStage": [ 63119.5, 20690.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos3", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.405], "Focus": [16480.2], "XYStage": [ 62321, 20690.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos4", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.415], "Focus": [16480], "XYStage": [ 56604.100000000006, 20688.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos5", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.42000000000002], "Focus": [16480.1], "XYStage": [ 56034.200000000004, 20688.800000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos6", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.455], "Focus": [16480], "XYStage": [ 50889.3, 20496.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos7", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.43], "Focus": [16480.2], "XYStage": [ 50101.600000000006, 20496.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos8", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.67000000000002], "Focus": [16479.9], "XYStage": [ 44818.600000000006, 20496.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos9", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.67000000000002], "Focus": [16480], "XYStage": [ 43897.9, 20436.800000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos10", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.675], "Focus": [16479.9], "XYStage": [ 39670.600000000006, 20664.9 ] }, "GridColumnIndex": 0 }, { "Label": "Pos11", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.69], "Focus": [16479.9], "XYStage": [ 38734, 20664.600000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos12", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.695], "Focus": [16480.1], "XYStage": [ 34037.200000000004, 20664.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos13", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.685], "Focus": [16480.1], "XYStage": [ 33152, 20664.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos14", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.69], "Focus": [16480.1], "XYStage": [ 28122.800000000003, 20664.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos15", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.675], "Focus": [16480], "XYStage": [ 27422.9, 20664.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos16", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.64000000000001], "Focus": [16480], "XYStage": [ 23005.2, 20664.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos17", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.69], "Focus": [16479.9], "XYStage": [ 21820.600000000002, 20409.600000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos18", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.41], "Focus": [16480.1], "XYStage": [ 73697.6, -13222.800000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos19", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.455], "Focus": [16480.1], "XYStage": [ 72765.8, -13223 ] }, "GridColumnIndex": 0 }, { "Label": "Pos20", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.4], "Focus": [16480.2], "XYStage": [ 67445.5, -12303.800000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos21", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.415], "Focus": [16480.2], "XYStage": [ 66613.3, -12303.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos22", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.415], "Focus": [16480.1], "XYStage": [ 61819.4, -11936.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos23", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.405], "Focus": [16480.2], "XYStage": [ 61325.700000000004, -11937.1 ] }, "GridColumnIndex": 0 }, { "Label": "Pos24", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.46], "Focus": [16480.2], "XYStage": [ 55377, -10974.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos25", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.445], "Focus": [16480.1], "XYStage": [ 54827, -11214.800000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos26", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.435], "Focus": [16480.2], "XYStage": [ 49937.100000000006, -10919.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos27", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.425], "Focus": [16480.1], "XYStage": [ 48764, -10919.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos28", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.42000000000002], "Focus": [16480.2], "XYStage": [ 43978.4, -10920.400000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos29", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.435], "Focus": [16480.2], "XYStage": [ 43106.3, -10920.400000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos30", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.405], "Focus": [16480.3], "XYStage": [ 38212.4, -10920.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos31", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.445], "Focus": [16480.2], "XYStage": [ 37254.9, -10920.400000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos32", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.44], "Focus": [16480.3], "XYStage": [ 32472.800000000003, -11294.400000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos33", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [166.95000000000002], "Focus": [16480.2], "XYStage": [ 31420.7, -11294.400000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos34", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [166.95000000000002], "Focus": [16480.2], "XYStage": [ 26881.5, -11640 ] }, "GridColumnIndex": 0 }, { "Label": "Pos35", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [166.98], "Focus": [16480.2], "XYStage": [ 25876.100000000002, -11641.1 ] }, "GridColumnIndex": 0 } ], "z-step_um": 10, "GridRow": 0, "BitDepth": 16, "Depth": 2, "Channels": 6, "Source": "Micro-Manager", "ChColors": [ -16711681, -256, -65281, -1, -1, -16776961 ], "MetadataVersion": 10, "KeepShutterOpenChannels": true, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536, 65536 ], "CustomIntervals_ms": [], "Height": 1200, "MicroManagerVersion": "1.4.23 20180219", "GridColumn": 0, "UUID": "8c8f9d20-b128-4577-9f91-89e70f22d319", "PixelType": "GRAY16", "Width": 1200, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\Yan\\20191217-YC113-S5S6", "Frames": 1, "Comment": "", "UserName": "Nikon", "PixelSize_um": 1.1, "ROI": [ 0, 0, 1200, 1200 ], "Date": "2019-12-17", "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC", "P-DAPI-high" ], "KeepShutterOpenSlices": true, "IJType": 1, "ComputerName": "ZadorConfocal2", "Positions": 36, "PositionIndex": 3, "CameraTimeout": 20000, "TimeFirst": false }, "640 AO-Name": "AnalogIO", "Vincent-D3-Command": "Close" }, "FrameKey-0-1-9": { "COM4-StopBits": "1", "528 AO-MaxVolts": "5.0000", "XLIGHT Excitation Wheel-State": "2", "470 Shutter-Name": "AnalogIO", "Core-Shutter": "555", "555 AO-Demo": "No", "XLIGHT Dichroic-State": "2", "XYStage-StepSize-Y": "0.1000", "ZPositionUm": 217.405, "XYStage-StepSize-X": "0.1000", "PRIME-FirmwareVersion": "20.28", "PRIME-ClearCycles": "2", "520-Name": "DA Shutter", "405 Shutter-Name": "AnalogIO", "PIEZO-StepSize": "0.0050", "PRIME-ClearMode": "Pre-Exposure", "470 AO-MinVolts": "0.0000", "405 AO-SupportsTriggering": "1", "CameraChannelIndex": 0, "XYStage-StartSpeed": "500.0000", "COM9-StopBits": "2", "405 AO-Description": "NI DAC", "COM4-DelayBetweenCharsMs": "0.0000", "445 AO-Block voltage": "false", "520 AO-TriggerInputLine": "/Dev1/PFI0", "555 AO-Description": "NI DAC", "520 AO-TriggerSampleRate": "100000", "COM4-Verbose": "1", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM4-Name": "COM4", "405 Shutter-Volts": "0.0000", "Vincent-D3-Port": "COM1", "PRIME-PP 4 MIN ADU AFFECTED": "200", "470 Shutter-MinVolts": "0.0000", "PRIME-PP 3 ENABLED": "Yes", "470 AO-Description": "NI DAC", "PRIME-Description": "PVCAM API device adapter", "520 Shutter-MaxVolts": "5.0000", "520 Shutter-Volts": "0.0000", "528 AO-Sequenceable": "1", "PRIME-Binning": "1x1", "555 Shutter-MaxVolts": "5.0000", "528 Shutter-TriggerSequenceLength": "1000", "405 Shutter-Block voltage": "false", "XLIGHT Spin motor-State": "1", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "Vincent-D3-Time to open (ms)": "35.0000", "Vincent-D3-Time to close (ms)": "35.0000", "445 AO-TriggerSampleRate": "100000", "640 Shutter-IOChannel": "Dev1/ao14", "555 AO-Sequenceable": "1", "520 AO-Name": "AnalogIO", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "470 AO-Block voltage": "false", "COM1-Verbose": "1", "COM9-AnswerTimeout": "10000.0000", "528 Shutter-Name": "AnalogIO", "PRIME-Trigger Timeout (secs)": "10", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "NextFrame": 0, "470 AO-TriggerSequenceLength": "1000", "PRIME-TransposeXY": "0", "445 AO-TriggerSequenceLength": "1000", "COM1-Name": "COM1", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "COM9-DataBits": "8", "COM1-AnswerTimeout": "500.0000", "Camera": "", "TE2000-Version": "V2.50-V2.50-V2.10", "528 Shutter-Volts": "0.0000", "XLIGHT Hub-Name": "XLIGHT Hub", "PIEZO-Description": "Ludl stage driver adapter", "PositionName": "Pos3", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSampleRate": "100000", "PRIME-FanSpeedSetpoint": "High", "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "405 AO-TriggerSampleRate": "100000", "470 Shutter-Block voltage": "false", "470-State": "0", "COM3-Parity": "None", "405 AO-Volts": "0.0000", "PRIME-TransposeMirrorY": "0", "PRIME-ChipName": "GS144BSI", "470 AO-Volts": "0.0000", "PRIME-TransposeMirrorX": "0", "445 Shutter-SupportsTriggering": "0", "470 Shutter-SupportsTriggering": "0", "XLIGHT Hub-Description": "XLIGHT hub", "PRIME-PreampOffLimit": "10000", "470 AO-Sequenceable": "1", "528 Shutter-MaxVolts": "5.0000", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "Core-XYStage": "XYStage", "470 AO-TriggerSampleRate": "100000", "PRIME-Timing-PreTriggerDelayNs": "24672000.0000", "528-DA Device": "528 Shutter", "Focus-Description": "Nikon TE2000 focus adapter", "FilterBlock-State": "5", "520 AO-Demo": "No", "COM3-DelayBetweenCharsMs": "0.0000", "470 Shutter-Volts": "0.0000", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "PRIME-PP 2 THRESHOLD": "125", "PRIME-PP 4 THRESHOLD": "75", "XYStage-Acceleration": "4.0000", "470 Shutter-Demo": "No", "PRIME-Name": "PMPCIECam00", "640-DA Device": "640 Shutter", "405 AO-Block voltage": "false", "ElapsedTime-ms": 142908, "PRIME-Offset": "100", "445 AO-IOChannel": "Dev1/ao1", "640 AO-MaxVolts": "5.0000", "PositionIndex": 3, "PRIME-PP 1 ENABLED": "Yes", "COM1-BaudRate": "9600", "640-State": "0", "COM3-Verbose": "1", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "LudlController-TransmissionDelay": "0", "PRIME-FullWellCapacity": "62000", "PRIME-SMARTStreamingEnabled": "No", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "640 Shutter-Description": "NI DAC", "528-Description": "DA device that is used as a shutter", "COM4-Description": "Serial port driver (boost:asio)", "405 Shutter-Description": "NI DAC", "TE2000-Name": "TE2000", "528 Shutter-Description": "NI DAC", "COM9-Description": "Serial port driver (boost:asio)", "LudlController-Name": "LudlController", "445 Shutter-TriggerSequenceLength": "1000", "PRIME-SerialNumber": "A18B203008", "445 AO-Volts": "0.0000", "PRIME-X-dimension": "1200", "PRIME-ShutterCloseDelay": "0", "XLIGHT Disk slider-State": "2", "Position": "Default", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 AO-IOChannel": "Dev1/ao5", "520 AO-IOChannel": "Dev1/ao4", "528 AO-TriggerSequenceLength": "1000", "405 Shutter-MaxVolts": "5.0000", "470-DA Device": "470 Shutter", "PRIME-FTCapable": "No", "Core-ChannelGroup": "Channels", "555 AO-SupportsTriggering": "0", "PRIME-Y-dimension": "1200", "Frame": 0, "555 Shutter-Sequenceable": "1", "PRIME-Timing-ExposureTimeNs": "9992000.0000", "Core-ImageProcessor": "", "528-State": "0", "520 AO-Block voltage": "false", "XLIGHT Excitation Wheel-Label": "QUAD", "640 Shutter-Demo": "No", "COM3-StopBits": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "520 Shutter-IOChannel": "Dev1/ao11", "Width": 1200, "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-SupportsTriggering": "0", "640 AO-TriggerSampleRate": "100000", "520-Description": "DA device that is used as a shutter", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "405 Shutter-Sequenceable": "1", "405 Shutter-SupportsTriggering": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-TriggerSampleRate": "100000", "640 Shutter-MaxVolts": "5.0000", "555 AO-MaxVolts": "5.0000", "520 AO-TriggerSequenceLength": "1000", "COM3-BaudRate": "9600", "COM9-BaudRate": "9600", "Vincent-D3-Channel #": "Ch. #1", "PRIME-PMode": "Normal", "OpticalPath-Label": "Left(100)", "BitDepth": 16, "555 AO-TriggerSequenceLength": "1000", "445 Shutter-Description": "NI DAC", "405 AO-Sequenceable": "1", "PRIME-AcquisitionMethod": "Callbacks", "555 AO-Name": "AnalogIO", "PRIME-ShutterMode": "Pre-Exposure", "COM3-Handshaking": "Off", "445 AO-SupportsTriggering": "0", "ROI": "0-0-1200-1200", "COM9-Verbose": "1", "PRIME-Color": "OFF", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "PRIME-ShutterOpenDelay": "0", "COM1-Parity": "None", "Slice": 9, "SlicePosition": 40, "COM9-DelayBetweenCharsMs": "0.0000", "555-Name": "DA Shutter", "PIEZO-LudlSingleAxisName": "B", "XLIGHT Emission Wheel-Label": "585/11(T)", "640 Shutter-Name": "AnalogIO", "640 AO-Sequenceable": "1", "520 Shutter-Demo": "No", "528 Shutter-Demo": "No", "470-Name": "DA Shutter", "520-DA Device": "520 Shutter", "XYStage-Description": "Ludl XY stage driver adapter", "445 Shutter-MinVolts": "0.0000", "528 AO-TriggerSampleRate": "100000", "405 AO-MinVolts": "0.0000", "405 Shutter-TriggerSequenceLength": "1000", "Height": 1200, "555 AO-Block voltage": "false", "640 Shutter-Volts": "0.0000", "PixelType": "GRAY16", "445 Shutter-Block voltage": "false", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "520 Shutter-TriggerSequenceLength": "1000", "COM9-Name": "COM9", "Core-SLM": "", "555 AO-Volts": "4.5000", "555 Shutter-Block voltage": "false", "XYStage-Speed": "1800.0000", "520 AO-MaxVolts": "5.0000", "528 Shutter-SupportsTriggering": "0", "COM1-Description": "Serial port driver (boost:asio)", "PRIME-MetadataEnabled": "No", "520 Shutter-MinVolts": "0.0000", "470 Shutter-Sequenceable": "1", "PRIME-PreampDelay": "0", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-MinVolts": "0.0000", "ChannelIndex": 1, "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Core-AutoShutter": "1", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "Exposure-ms": 160, "PRIME-PP 2 ENABLED": "Yes", "640 Shutter-TriggerSampleRate": "100000", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "470 Shutter-IOChannel": "Dev1/ao10", "405 AO-Demo": "No", "PixelSizeUm": 1.1, "470 AO-Demo": "No", "470 Shutter-MaxVolts": "5.0000", "Vincent-D3-Name": "Vincent-D3", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FileName": "img_000000000_Il-T_009.tif", "LudlController-Reset": "Operate", "PRIME-Exposure": "160.00", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "445-Name": "DA Shutter", "555 Shutter-Name": "AnalogIO", "520 Shutter-TriggerSampleRate": "100000", "445 Shutter-IOChannel": "Dev1/ao9", "PRIME-TransposeCorrection": "0", "XYStage-StepSize": "0.1000", "445-Description": "DA device that is used as a shutter", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 AO-IOChannel": "Dev1/ao7", "520 Shutter-Name": "AnalogIO", "COM3-DataBits": "8", "640 AO-Volts": "0.0000", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "555 Shutter-IOChannel": "Dev1/ao13", "WaitInterval": null, "520 Shutter-Sequenceable": "1", "YPositionUm": 20690.300000000003, "445-State": "0", "445-DA Device": "445 Shutter", "PRIME-CircularBufferAutoSize": "ON", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "555 AO-TriggerSampleRate": "100000", "445 Shutter-Volts": "0.0000", "XLIGHT Hub-Port": "COM3", "Core-AutoFocus": "", "COM4-Handshaking": "Off", "528 Shutter-MinVolts": "0.0000", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "445 Shutter-Name": "AnalogIO", "528 Shutter-Sequenceable": "1", "XYStage-TransposeMirrorX": "0", "XYStage-TransposeMirrorY": "0", "PRIME-ReadoutRate": "100MHz 16bit", "XYStage-Name": "Stage", "640 AO-SupportsTriggering": "0", "640-Name": "DA Shutter", "640 Shutter-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "640 AO-Block voltage": "false", "445 Shutter-Sequenceable": "1", "470 AO-MaxVolts": "5.0000", "COM4-BaudRate": "9600", "470-Description": "DA device that is used as a shutter", "520 Shutter-Description": "NI DAC", "PRIME-CCDTemperature": "-14.9600", "Time": "2019-12-17 21:58:50 -0500", "640 AO-TriggerSequenceLength": "1000", "470 AO-Name": "AnalogIO", "XLIGHT Disk slider-Label": "Disk Pos 40um", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "Binning": "1x1", "TE2000-ModelType": "1", "COM3-AnswerTimeout": "500.0000", "555 Shutter-SupportsTriggering": "0", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "TE2000-Port": "COM4", "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Description": "NI DAC", "COM4-AnswerTimeout": "500.0000", "COM1-DelayBetweenCharsMs": "0.0000", "FilterBlock-Label": "Confocal", "445 AO-Name": "AnalogIO", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-Timing-ReadoutTimeNs": "24672000.0000", "528 AO-Demo": "No", "520 AO-Sequenceable": "1", "555-Description": "DA device that is used as a shutter", "405-DA Device": "405 Shutter", "405 AO-TriggerSequenceLength": "1000", "640 AO-MinVolts": "0.0000", "640 Shutter-Block voltage": "false", "FrameIndex": 0, "PRIME-Port": "CMOS", "555 AO-MinVolts": "0.0000", "COM3-Description": "Serial port driver (boost:asio)", "528 AO-Description": "NI DAC", "555 AO-IOChannel": "Dev1/ao6", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "555 AO-TriggerInputLine": "/Dev1/PFI0", "PRIME-ActualInterval-ms": "374.3700", "LudlController-Port": "COM9", "445 Shutter-Demo": "No", "PRIME-Trigger-Expose Out-Mux": "1", "SliceIndex": 9, "PRIME-CCDTemperatureSetPoint": "-15.0000", "405 Shutter-MinVolts": "0.0000", "PRIME-ExposeOutMode": "First Row", "AxisPositions": null, "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Volts": "5.0000", "528-Name": "DA Shutter", "555-State": "1", "XLIGHT Dichroic-Label": "QUAD", "445 AO-Sequenceable": "1", "405-Name": "DA Shutter", "405 AO-IOChannel": "Dev1/ao0", "Core-Camera": "PRIME", "Focus-Name": "Focus", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "405 Shutter-IOChannel": "Dev1/ao8", "640 AO-Demo": "No", "640 Shutter-SupportsTriggering": "0", "445 AO-Demo": "No", "555-DA Device": "555 Shutter", "470 Shutter-Description": "NI DAC", "528 AO-Volts": "0.0000", "COM1-DataBits": "8", "Vincent-D3-Address": "x", "520 AO-SupportsTriggering": "0", "405-Description": "DA device that is used as a shutter", "640 Shutter-MinVolts": "0.0000", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "405 AO-Name": "AnalogIO", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "640 AO-TriggerInputLine": "/Dev1/PFI0", "405 AO-TriggerInputLine": "/Dev1/PFI0", "640-Description": "DA device that is used as a shutter", "445 Shutter-TriggerSampleRate": "100000", "470 Shutter-TriggerSequenceLength": "1000", "COM1-StopBits": "1", "520 Shutter-SupportsTriggering": "0", "405-State": "0", "Core-Initialize": "1", "XLIGHT Emission Wheel-State": "7", "520 AO-Description": "NI DAC", "528 AO-TriggerInputLine": "/Dev1/PFI0", "445 Shutter-MaxVolts": "5.0000", "COM9-Handshaking": "Off", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "XLIGHT Spin motor-Label": "On (spinning)", "PRIME-PP 5 ENABLED": "No", "UUID": "f9e2c841-f568-4a8d-b5c5-c585bb90146b", "PRIME-TriggerMode": "Internal Trigger", "COM1-Handshaking": "Off", "PRIME-Timing-PostTriggerDelayNs": "49344000.0000", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "Focus-Position": "16479.8000", "528 Shutter-Block voltage": "false", "Channel": "Il-T", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "555 Shutter-Demo": "No", "405 Shutter-Demo": "No", "528 AO-Name": "AnalogIO", "520-State": "0", "Core-Galvo": "", "405 Shutter-TriggerSampleRate": "100000", "Focus-FocusStepSize_(nm)": "100", "PRIME-PixelType": "16bit", "COM4-DataBits": "8", "470 AO-IOChannel": "Dev1/ao2", "520 AO-MinVolts": "0.0000", "COM9-Parity": "None", "PRIME-CircularBufferEnabled": "ON", "PRIME-PP 6 Reset": "No", "640 AO-Description": "NI DAC", "Source": "PRIME", "445 AO-MinVolts": "0.0000", "PIEZO-Name": "Stage", "445 AO-Description": "NI DAC", "PRIME-CircularBufferFrameCount": "46", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "520 AO-Volts": "0.0000", "528 Shutter-TriggerSampleRate": "100000", "XPositionUm": 62321, "OpticalPath-State": "4", "PRIME-PP 3 THRESHOLD": "80", "PRIME-PVCAM Version": "3.7.1", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Gain": "1-HDR", "640 Shutter-TriggerSequenceLength": "1000", "528 AO-SupportsTriggering": "0", "445 AO-TriggerInputLine": "/Dev1/PFI0", "OpticalPath-Name": "OpticalPath", "PRIME-PP 4 ENABLED": "Yes", "PRIME-PVCAM Adapter Version": "1.3.29", "COM3-Name": "COM3", "405 AO-MaxVolts": "5.0000", "520 Shutter-Block voltage": "false", "PRIME-Timing-ClearingTimeNs": "49344000.0000", "Core-Focus": "PIEZO", "528 AO-Block voltage": "false", "TE2000-Description": "Nikon TE2000 hub", "528 AO-MinVolts": "0.0000", "COM4-Parity": "None", "Summary": { "Slices": 11, "ChContrastMin": [ 0, 0, 0, 0, 0, 0 ], "PixelAspect": 1, "SlicesFirst": true, "Interval_ms": 0, "Time": "2019-12-17 21:58:39 -0500", "Prefix": "seq_3", "InitialPositionList": [ { "Label": "Pos0", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.435], "Focus": [16480.2], "XYStage": [ 69822.40000000001, 20100.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos1", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.425], "Focus": [16480.3], "XYStage": [ 69004, 20101 ] }, "GridColumnIndex": 0 }, { "Label": "Pos2", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.44], "Focus": [16480.1], "XYStage": [ 63119.5, 20690.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos3", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.405], "Focus": [16480.2], "XYStage": [ 62321, 20690.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos4", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.415], "Focus": [16480], "XYStage": [ 56604.100000000006, 20688.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos5", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.42000000000002], "Focus": [16480.1], "XYStage": [ 56034.200000000004, 20688.800000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos6", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.455], "Focus": [16480], "XYStage": [ 50889.3, 20496.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos7", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.43], "Focus": [16480.2], "XYStage": [ 50101.600000000006, 20496.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos8", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.67000000000002], "Focus": [16479.9], "XYStage": [ 44818.600000000006, 20496.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos9", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.67000000000002], "Focus": [16480], "XYStage": [ 43897.9, 20436.800000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos10", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.675], "Focus": [16479.9], "XYStage": [ 39670.600000000006, 20664.9 ] }, "GridColumnIndex": 0 }, { "Label": "Pos11", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.69], "Focus": [16479.9], "XYStage": [ 38734, 20664.600000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos12", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.695], "Focus": [16480.1], "XYStage": [ 34037.200000000004, 20664.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos13", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.685], "Focus": [16480.1], "XYStage": [ 33152, 20664.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos14", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.69], "Focus": [16480.1], "XYStage": [ 28122.800000000003, 20664.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos15", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.675], "Focus": [16480], "XYStage": [ 27422.9, 20664.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos16", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.64000000000001], "Focus": [16480], "XYStage": [ 23005.2, 20664.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos17", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.69], "Focus": [16479.9], "XYStage": [ 21820.600000000002, 20409.600000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos18", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.41], "Focus": [16480.1], "XYStage": [ 73697.6, -13222.800000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos19", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.455], "Focus": [16480.1], "XYStage": [ 72765.8, -13223 ] }, "GridColumnIndex": 0 }, { "Label": "Pos20", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.4], "Focus": [16480.2], "XYStage": [ 67445.5, -12303.800000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos21", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.415], "Focus": [16480.2], "XYStage": [ 66613.3, -12303.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos22", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.415], "Focus": [16480.1], "XYStage": [ 61819.4, -11936.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos23", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.405], "Focus": [16480.2], "XYStage": [ 61325.700000000004, -11937.1 ] }, "GridColumnIndex": 0 }, { "Label": "Pos24", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.46], "Focus": [16480.2], "XYStage": [ 55377, -10974.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos25", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.445], "Focus": [16480.1], "XYStage": [ 54827, -11214.800000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos26", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.435], "Focus": [16480.2], "XYStage": [ 49937.100000000006, -10919.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos27", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.425], "Focus": [16480.1], "XYStage": [ 48764, -10919.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos28", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.42000000000002], "Focus": [16480.2], "XYStage": [ 43978.4, -10920.400000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos29", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.435], "Focus": [16480.2], "XYStage": [ 43106.3, -10920.400000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos30", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.405], "Focus": [16480.3], "XYStage": [ 38212.4, -10920.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos31", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.445], "Focus": [16480.2], "XYStage": [ 37254.9, -10920.400000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos32", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.44], "Focus": [16480.3], "XYStage": [ 32472.800000000003, -11294.400000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos33", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [166.95000000000002], "Focus": [16480.2], "XYStage": [ 31420.7, -11294.400000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos34", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [166.95000000000002], "Focus": [16480.2], "XYStage": [ 26881.5, -11640 ] }, "GridColumnIndex": 0 }, { "Label": "Pos35", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [166.98], "Focus": [16480.2], "XYStage": [ 25876.100000000002, -11641.1 ] }, "GridColumnIndex": 0 } ], "z-step_um": 10, "GridRow": 0, "BitDepth": 16, "Depth": 2, "Channels": 6, "Source": "Micro-Manager", "ChColors": [ -16711681, -256, -65281, -1, -1, -16776961 ], "MetadataVersion": 10, "KeepShutterOpenChannels": true, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536, 65536 ], "CustomIntervals_ms": [], "Height": 1200, "MicroManagerVersion": "1.4.23 20180219", "GridColumn": 0, "UUID": "8c8f9d20-b128-4577-9f91-89e70f22d319", "PixelType": "GRAY16", "Width": 1200, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\Yan\\20191217-YC113-S5S6", "Frames": 1, "Comment": "", "UserName": "Nikon", "PixelSize_um": 1.1, "ROI": [ 0, 0, 1200, 1200 ], "Date": "2019-12-17", "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC", "P-DAPI-high" ], "KeepShutterOpenSlices": true, "IJType": 1, "ComputerName": "ZadorConfocal2", "Positions": 36, "PositionIndex": 3, "CameraTimeout": 20000, "TimeFirst": false }, "640 AO-Name": "AnalogIO", "Vincent-D3-Command": "Close" }, "FrameKey-0-1-10": { "COM4-StopBits": "1", "528 AO-MaxVolts": "5.0000", "XLIGHT Excitation Wheel-State": "2", "470 Shutter-Name": "AnalogIO", "Core-Shutter": "555", "555 AO-Demo": "No", "XLIGHT Dichroic-State": "2", "XYStage-StepSize-Y": "0.1000", "ZPositionUm": 227.405, "XYStage-StepSize-X": "0.1000", "PRIME-FirmwareVersion": "20.28", "PRIME-ClearCycles": "2", "520-Name": "DA Shutter", "405 Shutter-Name": "AnalogIO", "PIEZO-StepSize": "0.0050", "PRIME-ClearMode": "Pre-Exposure", "470 AO-MinVolts": "0.0000", "405 AO-SupportsTriggering": "1", "CameraChannelIndex": 0, "XYStage-StartSpeed": "500.0000", "COM9-StopBits": "2", "405 AO-Description": "NI DAC", "COM4-DelayBetweenCharsMs": "0.0000", "445 AO-Block voltage": "false", "520 AO-TriggerInputLine": "/Dev1/PFI0", "555 AO-Description": "NI DAC", "520 AO-TriggerSampleRate": "100000", "COM4-Verbose": "1", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM4-Name": "COM4", "405 Shutter-Volts": "0.0000", "Vincent-D3-Port": "COM1", "PRIME-PP 4 MIN ADU AFFECTED": "200", "470 Shutter-MinVolts": "0.0000", "PRIME-PP 3 ENABLED": "Yes", "470 AO-Description": "NI DAC", "PRIME-Description": "PVCAM API device adapter", "520 Shutter-MaxVolts": "5.0000", "520 Shutter-Volts": "0.0000", "528 AO-Sequenceable": "1", "PRIME-Binning": "1x1", "555 Shutter-MaxVolts": "5.0000", "528 Shutter-TriggerSequenceLength": "1000", "405 Shutter-Block voltage": "false", "XLIGHT Spin motor-State": "1", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "Vincent-D3-Time to open (ms)": "35.0000", "Vincent-D3-Time to close (ms)": "35.0000", "445 AO-TriggerSampleRate": "100000", "640 Shutter-IOChannel": "Dev1/ao14", "555 AO-Sequenceable": "1", "520 AO-Name": "AnalogIO", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "470 AO-Block voltage": "false", "COM1-Verbose": "1", "COM9-AnswerTimeout": "10000.0000", "528 Shutter-Name": "AnalogIO", "PRIME-Trigger Timeout (secs)": "10", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "NextFrame": 0, "470 AO-TriggerSequenceLength": "1000", "PRIME-TransposeXY": "0", "445 AO-TriggerSequenceLength": "1000", "COM1-Name": "COM1", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "COM9-DataBits": "8", "COM1-AnswerTimeout": "500.0000", "Camera": "", "TE2000-Version": "V2.50-V2.50-V2.10", "528 Shutter-Volts": "0.0000", "XLIGHT Hub-Name": "XLIGHT Hub", "PIEZO-Description": "Ludl stage driver adapter", "PositionName": "Pos3", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSampleRate": "100000", "PRIME-FanSpeedSetpoint": "High", "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "405 AO-TriggerSampleRate": "100000", "470 Shutter-Block voltage": "false", "470-State": "0", "COM3-Parity": "None", "405 AO-Volts": "0.0000", "PRIME-TransposeMirrorY": "0", "PRIME-ChipName": "GS144BSI", "470 AO-Volts": "0.0000", "PRIME-TransposeMirrorX": "0", "445 Shutter-SupportsTriggering": "0", "470 Shutter-SupportsTriggering": "0", "XLIGHT Hub-Description": "XLIGHT hub", "PRIME-PreampOffLimit": "10000", "470 AO-Sequenceable": "1", "528 Shutter-MaxVolts": "5.0000", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "Core-XYStage": "XYStage", "470 AO-TriggerSampleRate": "100000", "PRIME-Timing-PreTriggerDelayNs": "24672000.0000", "528-DA Device": "528 Shutter", "Focus-Description": "Nikon TE2000 focus adapter", "FilterBlock-State": "5", "520 AO-Demo": "No", "COM3-DelayBetweenCharsMs": "0.0000", "470 Shutter-Volts": "0.0000", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "PRIME-PP 2 THRESHOLD": "125", "PRIME-PP 4 THRESHOLD": "75", "XYStage-Acceleration": "4.0000", "470 Shutter-Demo": "No", "PRIME-Name": "PMPCIECam00", "640-DA Device": "640 Shutter", "405 AO-Block voltage": "false", "ElapsedTime-ms": 143303, "PRIME-Offset": "100", "445 AO-IOChannel": "Dev1/ao1", "640 AO-MaxVolts": "5.0000", "PositionIndex": 3, "PRIME-PP 1 ENABLED": "Yes", "COM1-BaudRate": "9600", "640-State": "0", "COM3-Verbose": "1", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "LudlController-TransmissionDelay": "0", "PRIME-FullWellCapacity": "62000", "PRIME-SMARTStreamingEnabled": "No", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "640 Shutter-Description": "NI DAC", "528-Description": "DA device that is used as a shutter", "COM4-Description": "Serial port driver (boost:asio)", "405 Shutter-Description": "NI DAC", "TE2000-Name": "TE2000", "528 Shutter-Description": "NI DAC", "COM9-Description": "Serial port driver (boost:asio)", "LudlController-Name": "LudlController", "445 Shutter-TriggerSequenceLength": "1000", "PRIME-SerialNumber": "A18B203008", "445 AO-Volts": "0.0000", "PRIME-X-dimension": "1200", "PRIME-ShutterCloseDelay": "0", "XLIGHT Disk slider-State": "2", "Position": "Default", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 AO-IOChannel": "Dev1/ao5", "520 AO-IOChannel": "Dev1/ao4", "528 AO-TriggerSequenceLength": "1000", "405 Shutter-MaxVolts": "5.0000", "470-DA Device": "470 Shutter", "PRIME-FTCapable": "No", "Core-ChannelGroup": "Channels", "555 AO-SupportsTriggering": "0", "PRIME-Y-dimension": "1200", "Frame": 0, "555 Shutter-Sequenceable": "1", "PRIME-Timing-ExposureTimeNs": "9992000.0000", "Core-ImageProcessor": "", "528-State": "0", "520 AO-Block voltage": "false", "XLIGHT Excitation Wheel-Label": "QUAD", "640 Shutter-Demo": "No", "COM3-StopBits": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "520 Shutter-IOChannel": "Dev1/ao11", "Width": 1200, "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-SupportsTriggering": "0", "640 AO-TriggerSampleRate": "100000", "520-Description": "DA device that is used as a shutter", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "405 Shutter-Sequenceable": "1", "405 Shutter-SupportsTriggering": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-TriggerSampleRate": "100000", "640 Shutter-MaxVolts": "5.0000", "555 AO-MaxVolts": "5.0000", "520 AO-TriggerSequenceLength": "1000", "COM3-BaudRate": "9600", "COM9-BaudRate": "9600", "Vincent-D3-Channel #": "Ch. #1", "PRIME-PMode": "Normal", "OpticalPath-Label": "Left(100)", "BitDepth": 16, "555 AO-TriggerSequenceLength": "1000", "445 Shutter-Description": "NI DAC", "405 AO-Sequenceable": "1", "PRIME-AcquisitionMethod": "Callbacks", "555 AO-Name": "AnalogIO", "PRIME-ShutterMode": "Pre-Exposure", "COM3-Handshaking": "Off", "445 AO-SupportsTriggering": "0", "ROI": "0-0-1200-1200", "COM9-Verbose": "1", "PRIME-Color": "OFF", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "PRIME-ShutterOpenDelay": "0", "COM1-Parity": "None", "Slice": 10, "SlicePosition": 50, "COM9-DelayBetweenCharsMs": "0.0000", "555-Name": "DA Shutter", "PIEZO-LudlSingleAxisName": "B", "XLIGHT Emission Wheel-Label": "585/11(T)", "640 Shutter-Name": "AnalogIO", "640 AO-Sequenceable": "1", "520 Shutter-Demo": "No", "528 Shutter-Demo": "No", "470-Name": "DA Shutter", "520-DA Device": "520 Shutter", "XYStage-Description": "Ludl XY stage driver adapter", "445 Shutter-MinVolts": "0.0000", "528 AO-TriggerSampleRate": "100000", "405 AO-MinVolts": "0.0000", "405 Shutter-TriggerSequenceLength": "1000", "Height": 1200, "555 AO-Block voltage": "false", "640 Shutter-Volts": "0.0000", "PixelType": "GRAY16", "445 Shutter-Block voltage": "false", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "520 Shutter-TriggerSequenceLength": "1000", "COM9-Name": "COM9", "Core-SLM": "", "555 AO-Volts": "4.5000", "555 Shutter-Block voltage": "false", "XYStage-Speed": "1800.0000", "520 AO-MaxVolts": "5.0000", "528 Shutter-SupportsTriggering": "0", "COM1-Description": "Serial port driver (boost:asio)", "PRIME-MetadataEnabled": "No", "520 Shutter-MinVolts": "0.0000", "470 Shutter-Sequenceable": "1", "PRIME-PreampDelay": "0", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-MinVolts": "0.0000", "ChannelIndex": 1, "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Core-AutoShutter": "1", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "Exposure-ms": 160, "PRIME-PP 2 ENABLED": "Yes", "640 Shutter-TriggerSampleRate": "100000", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "470 Shutter-IOChannel": "Dev1/ao10", "405 AO-Demo": "No", "PixelSizeUm": 1.1, "470 AO-Demo": "No", "470 Shutter-MaxVolts": "5.0000", "Vincent-D3-Name": "Vincent-D3", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FileName": "img_000000000_Il-T_010.tif", "LudlController-Reset": "Operate", "PRIME-Exposure": "160.00", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "445-Name": "DA Shutter", "555 Shutter-Name": "AnalogIO", "520 Shutter-TriggerSampleRate": "100000", "445 Shutter-IOChannel": "Dev1/ao9", "PRIME-TransposeCorrection": "0", "XYStage-StepSize": "0.1000", "445-Description": "DA device that is used as a shutter", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 AO-IOChannel": "Dev1/ao7", "520 Shutter-Name": "AnalogIO", "COM3-DataBits": "8", "640 AO-Volts": "0.0000", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "555 Shutter-IOChannel": "Dev1/ao13", "WaitInterval": null, "520 Shutter-Sequenceable": "1", "YPositionUm": 20690.300000000003, "445-State": "0", "445-DA Device": "445 Shutter", "PRIME-CircularBufferAutoSize": "ON", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "555 AO-TriggerSampleRate": "100000", "445 Shutter-Volts": "0.0000", "XLIGHT Hub-Port": "COM3", "Core-AutoFocus": "", "COM4-Handshaking": "Off", "528 Shutter-MinVolts": "0.0000", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "445 Shutter-Name": "AnalogIO", "528 Shutter-Sequenceable": "1", "XYStage-TransposeMirrorX": "0", "XYStage-TransposeMirrorY": "0", "PRIME-ReadoutRate": "100MHz 16bit", "XYStage-Name": "Stage", "640 AO-SupportsTriggering": "0", "640-Name": "DA Shutter", "640 Shutter-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "640 AO-Block voltage": "false", "445 Shutter-Sequenceable": "1", "470 AO-MaxVolts": "5.0000", "COM4-BaudRate": "9600", "470-Description": "DA device that is used as a shutter", "520 Shutter-Description": "NI DAC", "PRIME-CCDTemperature": "-14.9600", "Time": "2019-12-17 21:58:50 -0500", "640 AO-TriggerSequenceLength": "1000", "470 AO-Name": "AnalogIO", "XLIGHT Disk slider-Label": "Disk Pos 40um", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "Binning": "1x1", "TE2000-ModelType": "1", "COM3-AnswerTimeout": "500.0000", "555 Shutter-SupportsTriggering": "0", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "TE2000-Port": "COM4", "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Description": "NI DAC", "COM4-AnswerTimeout": "500.0000", "COM1-DelayBetweenCharsMs": "0.0000", "FilterBlock-Label": "Confocal", "445 AO-Name": "AnalogIO", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-Timing-ReadoutTimeNs": "24672000.0000", "528 AO-Demo": "No", "520 AO-Sequenceable": "1", "555-Description": "DA device that is used as a shutter", "405-DA Device": "405 Shutter", "405 AO-TriggerSequenceLength": "1000", "640 AO-MinVolts": "0.0000", "640 Shutter-Block voltage": "false", "FrameIndex": 0, "PRIME-Port": "CMOS", "555 AO-MinVolts": "0.0000", "COM3-Description": "Serial port driver (boost:asio)", "528 AO-Description": "NI DAC", "555 AO-IOChannel": "Dev1/ao6", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "555 AO-TriggerInputLine": "/Dev1/PFI0", "PRIME-ActualInterval-ms": "374.3700", "LudlController-Port": "COM9", "445 Shutter-Demo": "No", "PRIME-Trigger-Expose Out-Mux": "1", "SliceIndex": 10, "PRIME-CCDTemperatureSetPoint": "-15.0000", "405 Shutter-MinVolts": "0.0000", "PRIME-ExposeOutMode": "First Row", "AxisPositions": null, "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Volts": "5.0000", "528-Name": "DA Shutter", "555-State": "0", "XLIGHT Dichroic-Label": "QUAD", "445 AO-Sequenceable": "1", "405-Name": "DA Shutter", "405 AO-IOChannel": "Dev1/ao0", "Core-Camera": "PRIME", "Focus-Name": "Focus", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "405 Shutter-IOChannel": "Dev1/ao8", "640 AO-Demo": "No", "640 Shutter-SupportsTriggering": "0", "445 AO-Demo": "No", "555-DA Device": "555 Shutter", "470 Shutter-Description": "NI DAC", "528 AO-Volts": "0.0000", "COM1-DataBits": "8", "Vincent-D3-Address": "x", "520 AO-SupportsTriggering": "0", "405-Description": "DA device that is used as a shutter", "640 Shutter-MinVolts": "0.0000", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "405 AO-Name": "AnalogIO", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "640 AO-TriggerInputLine": "/Dev1/PFI0", "405 AO-TriggerInputLine": "/Dev1/PFI0", "640-Description": "DA device that is used as a shutter", "445 Shutter-TriggerSampleRate": "100000", "470 Shutter-TriggerSequenceLength": "1000", "COM1-StopBits": "1", "520 Shutter-SupportsTriggering": "0", "405-State": "0", "Core-Initialize": "1", "XLIGHT Emission Wheel-State": "7", "520 AO-Description": "NI DAC", "528 AO-TriggerInputLine": "/Dev1/PFI0", "445 Shutter-MaxVolts": "5.0000", "COM9-Handshaking": "Off", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "XLIGHT Spin motor-Label": "On (spinning)", "PRIME-PP 5 ENABLED": "No", "UUID": "b4356577-6343-4e0d-b243-66e60b37692a", "PRIME-TriggerMode": "Internal Trigger", "COM1-Handshaking": "Off", "PRIME-Timing-PostTriggerDelayNs": "49344000.0000", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "Focus-Position": "16479.8000", "528 Shutter-Block voltage": "false", "Channel": "Il-T", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "555 Shutter-Demo": "No", "405 Shutter-Demo": "No", "528 AO-Name": "AnalogIO", "520-State": "0", "Core-Galvo": "", "405 Shutter-TriggerSampleRate": "100000", "Focus-FocusStepSize_(nm)": "100", "PRIME-PixelType": "16bit", "COM4-DataBits": "8", "470 AO-IOChannel": "Dev1/ao2", "520 AO-MinVolts": "0.0000", "COM9-Parity": "None", "PRIME-CircularBufferEnabled": "ON", "PRIME-PP 6 Reset": "No", "640 AO-Description": "NI DAC", "Source": "PRIME", "445 AO-MinVolts": "0.0000", "PIEZO-Name": "Stage", "445 AO-Description": "NI DAC", "PRIME-CircularBufferFrameCount": "46", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "520 AO-Volts": "0.0000", "528 Shutter-TriggerSampleRate": "100000", "XPositionUm": 62321, "OpticalPath-State": "4", "PRIME-PP 3 THRESHOLD": "80", "PRIME-PVCAM Version": "3.7.1", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Gain": "1-HDR", "640 Shutter-TriggerSequenceLength": "1000", "528 AO-SupportsTriggering": "0", "445 AO-TriggerInputLine": "/Dev1/PFI0", "OpticalPath-Name": "OpticalPath", "PRIME-PP 4 ENABLED": "Yes", "PRIME-PVCAM Adapter Version": "1.3.29", "COM3-Name": "COM3", "405 AO-MaxVolts": "5.0000", "520 Shutter-Block voltage": "false", "PRIME-Timing-ClearingTimeNs": "49344000.0000", "Core-Focus": "PIEZO", "528 AO-Block voltage": "false", "TE2000-Description": "Nikon TE2000 hub", "528 AO-MinVolts": "0.0000", "COM4-Parity": "None", "Summary": { "Slices": 11, "ChContrastMin": [ 0, 0, 0, 0, 0, 0 ], "PixelAspect": 1, "SlicesFirst": true, "Interval_ms": 0, "Time": "2019-12-17 21:58:39 -0500", "Prefix": "seq_3", "InitialPositionList": [ { "Label": "Pos0", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.435], "Focus": [16480.2], "XYStage": [ 69822.40000000001, 20100.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos1", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.425], "Focus": [16480.3], "XYStage": [ 69004, 20101 ] }, "GridColumnIndex": 0 }, { "Label": "Pos2", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.44], "Focus": [16480.1], "XYStage": [ 63119.5, 20690.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos3", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.405], "Focus": [16480.2], "XYStage": [ 62321, 20690.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos4", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.415], "Focus": [16480], "XYStage": [ 56604.100000000006, 20688.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos5", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.42000000000002], "Focus": [16480.1], "XYStage": [ 56034.200000000004, 20688.800000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos6", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.455], "Focus": [16480], "XYStage": [ 50889.3, 20496.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos7", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.43], "Focus": [16480.2], "XYStage": [ 50101.600000000006, 20496.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos8", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.67000000000002], "Focus": [16479.9], "XYStage": [ 44818.600000000006, 20496.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos9", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.67000000000002], "Focus": [16480], "XYStage": [ 43897.9, 20436.800000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos10", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.675], "Focus": [16479.9], "XYStage": [ 39670.600000000006, 20664.9 ] }, "GridColumnIndex": 0 }, { "Label": "Pos11", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.69], "Focus": [16479.9], "XYStage": [ 38734, 20664.600000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos12", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.695], "Focus": [16480.1], "XYStage": [ 34037.200000000004, 20664.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos13", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.685], "Focus": [16480.1], "XYStage": [ 33152, 20664.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos14", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.69], "Focus": [16480.1], "XYStage": [ 28122.800000000003, 20664.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos15", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.675], "Focus": [16480], "XYStage": [ 27422.9, 20664.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos16", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.64000000000001], "Focus": [16480], "XYStage": [ 23005.2, 20664.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos17", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.69], "Focus": [16479.9], "XYStage": [ 21820.600000000002, 20409.600000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos18", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.41], "Focus": [16480.1], "XYStage": [ 73697.6, -13222.800000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos19", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.455], "Focus": [16480.1], "XYStage": [ 72765.8, -13223 ] }, "GridColumnIndex": 0 }, { "Label": "Pos20", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.4], "Focus": [16480.2], "XYStage": [ 67445.5, -12303.800000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos21", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.415], "Focus": [16480.2], "XYStage": [ 66613.3, -12303.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos22", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.415], "Focus": [16480.1], "XYStage": [ 61819.4, -11936.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos23", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.405], "Focus": [16480.2], "XYStage": [ 61325.700000000004, -11937.1 ] }, "GridColumnIndex": 0 }, { "Label": "Pos24", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.46], "Focus": [16480.2], "XYStage": [ 55377, -10974.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos25", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.445], "Focus": [16480.1], "XYStage": [ 54827, -11214.800000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos26", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.435], "Focus": [16480.2], "XYStage": [ 49937.100000000006, -10919.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos27", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.425], "Focus": [16480.1], "XYStage": [ 48764, -10919.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos28", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.42000000000002], "Focus": [16480.2], "XYStage": [ 43978.4, -10920.400000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos29", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.435], "Focus": [16480.2], "XYStage": [ 43106.3, -10920.400000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos30", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.405], "Focus": [16480.3], "XYStage": [ 38212.4, -10920.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos31", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.445], "Focus": [16480.2], "XYStage": [ 37254.9, -10920.400000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos32", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.44], "Focus": [16480.3], "XYStage": [ 32472.800000000003, -11294.400000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos33", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [166.95000000000002], "Focus": [16480.2], "XYStage": [ 31420.7, -11294.400000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos34", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [166.95000000000002], "Focus": [16480.2], "XYStage": [ 26881.5, -11640 ] }, "GridColumnIndex": 0 }, { "Label": "Pos35", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [166.98], "Focus": [16480.2], "XYStage": [ 25876.100000000002, -11641.1 ] }, "GridColumnIndex": 0 } ], "z-step_um": 10, "GridRow": 0, "BitDepth": 16, "Depth": 2, "Channels": 6, "Source": "Micro-Manager", "ChColors": [ -16711681, -256, -65281, -1, -1, -16776961 ], "MetadataVersion": 10, "KeepShutterOpenChannels": true, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536, 65536 ], "CustomIntervals_ms": [], "Height": 1200, "MicroManagerVersion": "1.4.23 20180219", "GridColumn": 0, "UUID": "8c8f9d20-b128-4577-9f91-89e70f22d319", "PixelType": "GRAY16", "Width": 1200, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\Yan\\20191217-YC113-S5S6", "Frames": 1, "Comment": "", "UserName": "Nikon", "PixelSize_um": 1.1, "ROI": [ 0, 0, 1200, 1200 ], "Date": "2019-12-17", "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC", "P-DAPI-high" ], "KeepShutterOpenSlices": true, "IJType": 1, "ComputerName": "ZadorConfocal2", "Positions": 36, "PositionIndex": 3, "CameraTimeout": 20000, "TimeFirst": false }, "640 AO-Name": "AnalogIO", "Vincent-D3-Command": "Close" }, "FrameKey-0-2-0": { "COM4-StopBits": "1", "528 AO-MaxVolts": "5.0000", "XLIGHT Excitation Wheel-State": "2", "470 Shutter-Name": "AnalogIO", "Core-Shutter": "640", "555 AO-Demo": "No", "XLIGHT Dichroic-State": "2", "XYStage-StepSize-Y": "0.1000", "ZPositionUm": 127.405, "XYStage-StepSize-X": "0.1000", "PRIME-FirmwareVersion": "20.28", "PRIME-ClearCycles": "2", "520-Name": "DA Shutter", "405 Shutter-Name": "AnalogIO", "PIEZO-StepSize": "0.0050", "PRIME-ClearMode": "Pre-Exposure", "470 AO-MinVolts": "0.0000", "405 AO-SupportsTriggering": "1", "CameraChannelIndex": 0, "XYStage-StartSpeed": "500.0000", "COM9-StopBits": "2", "405 AO-Description": "NI DAC", "COM4-DelayBetweenCharsMs": "0.0000", "445 AO-Block voltage": "false", "520 AO-TriggerInputLine": "/Dev1/PFI0", "555 AO-Description": "NI DAC", "520 AO-TriggerSampleRate": "100000", "COM4-Verbose": "1", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM4-Name": "COM4", "405 Shutter-Volts": "0.0000", "Vincent-D3-Port": "COM1", "PRIME-PP 4 MIN ADU AFFECTED": "200", "470 Shutter-MinVolts": "0.0000", "PRIME-PP 3 ENABLED": "Yes", "470 AO-Description": "NI DAC", "PRIME-Description": "PVCAM API device adapter", "520 Shutter-MaxVolts": "5.0000", "520 Shutter-Volts": "0.0000", "528 AO-Sequenceable": "1", "PRIME-Binning": "1x1", "555 Shutter-MaxVolts": "5.0000", "528 Shutter-TriggerSequenceLength": "1000", "405 Shutter-Block voltage": "false", "XLIGHT Spin motor-State": "1", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "Vincent-D3-Time to open (ms)": "35.0000", "Vincent-D3-Time to close (ms)": "35.0000", "445 AO-TriggerSampleRate": "100000", "640 Shutter-IOChannel": "Dev1/ao14", "555 AO-Sequenceable": "1", "520 AO-Name": "AnalogIO", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "470 AO-Block voltage": "false", "COM1-Verbose": "1", "COM9-AnswerTimeout": "10000.0000", "528 Shutter-Name": "AnalogIO", "PRIME-Trigger Timeout (secs)": "10", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "NextFrame": 0, "470 AO-TriggerSequenceLength": "1000", "PRIME-TransposeXY": "0", "445 AO-TriggerSequenceLength": "1000", "COM1-Name": "COM1", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "COM9-DataBits": "8", "COM1-AnswerTimeout": "500.0000", "Camera": "", "TE2000-Version": "V2.50-V2.50-V2.10", "528 Shutter-Volts": "0.0000", "XLIGHT Hub-Name": "XLIGHT Hub", "PIEZO-Description": "Ludl stage driver adapter", "PositionName": "Pos3", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSampleRate": "100000", "PRIME-FanSpeedSetpoint": "High", "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "405 AO-TriggerSampleRate": "100000", "470 Shutter-Block voltage": "false", "470-State": "0", "COM3-Parity": "None", "405 AO-Volts": "0.0000", "PRIME-TransposeMirrorY": "0", "PRIME-ChipName": "GS144BSI", "470 AO-Volts": "0.0000", "PRIME-TransposeMirrorX": "0", "445 Shutter-SupportsTriggering": "0", "470 Shutter-SupportsTriggering": "0", "XLIGHT Hub-Description": "XLIGHT hub", "PRIME-PreampOffLimit": "10000", "470 AO-Sequenceable": "1", "528 Shutter-MaxVolts": "5.0000", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "Core-XYStage": "XYStage", "470 AO-TriggerSampleRate": "100000", "PRIME-Timing-PreTriggerDelayNs": "24672000.0000", "528-DA Device": "528 Shutter", "Focus-Description": "Nikon TE2000 focus adapter", "FilterBlock-State": "5", "520 AO-Demo": "No", "COM3-DelayBetweenCharsMs": "0.0000", "470 Shutter-Volts": "0.0000", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "PRIME-PP 2 THRESHOLD": "125", "PRIME-PP 4 THRESHOLD": "75", "XYStage-Acceleration": "4.0000", "470 Shutter-Demo": "No", "PRIME-Name": "PMPCIECam00", "640-DA Device": "640 Shutter", "405 AO-Block voltage": "false", "ElapsedTime-ms": 144461, "PRIME-Offset": "100", "445 AO-IOChannel": "Dev1/ao1", "640 AO-MaxVolts": "5.0000", "PositionIndex": 3, "PRIME-PP 1 ENABLED": "Yes", "COM1-BaudRate": "9600", "640-State": "1", "COM3-Verbose": "1", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "LudlController-TransmissionDelay": "0", "PRIME-FullWellCapacity": "62000", "PRIME-SMARTStreamingEnabled": "No", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "640 Shutter-Description": "NI DAC", "528-Description": "DA device that is used as a shutter", "COM4-Description": "Serial port driver (boost:asio)", "405 Shutter-Description": "NI DAC", "TE2000-Name": "TE2000", "528 Shutter-Description": "NI DAC", "COM9-Description": "Serial port driver (boost:asio)", "LudlController-Name": "LudlController", "445 Shutter-TriggerSequenceLength": "1000", "PRIME-SerialNumber": "A18B203008", "445 AO-Volts": "0.0000", "PRIME-X-dimension": "1200", "PRIME-ShutterCloseDelay": "0", "XLIGHT Disk slider-State": "2", "Position": "Default", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 AO-IOChannel": "Dev1/ao5", "520 AO-IOChannel": "Dev1/ao4", "528 AO-TriggerSequenceLength": "1000", "405 Shutter-MaxVolts": "5.0000", "470-DA Device": "470 Shutter", "PRIME-FTCapable": "No", "Core-ChannelGroup": "Channels", "555 AO-SupportsTriggering": "0", "PRIME-Y-dimension": "1200", "Frame": 0, "555 Shutter-Sequenceable": "1", "PRIME-Timing-ExposureTimeNs": "9992000.0000", "Core-ImageProcessor": "", "528-State": "0", "520 AO-Block voltage": "false", "XLIGHT Excitation Wheel-Label": "QUAD", "640 Shutter-Demo": "No", "COM3-StopBits": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "520 Shutter-IOChannel": "Dev1/ao11", "Width": 1200, "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-SupportsTriggering": "0", "640 AO-TriggerSampleRate": "100000", "520-Description": "DA device that is used as a shutter", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "405 Shutter-Sequenceable": "1", "405 Shutter-SupportsTriggering": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-TriggerSampleRate": "100000", "640 Shutter-MaxVolts": "5.0000", "555 AO-MaxVolts": "5.0000", "520 AO-TriggerSequenceLength": "1000", "COM3-BaudRate": "9600", "COM9-BaudRate": "9600", "Vincent-D3-Channel #": "Ch. #1", "PRIME-PMode": "Normal", "OpticalPath-Label": "Left(100)", "BitDepth": 16, "555 AO-TriggerSequenceLength": "1000", "445 Shutter-Description": "NI DAC", "405 AO-Sequenceable": "1", "PRIME-AcquisitionMethod": "Callbacks", "555 AO-Name": "AnalogIO", "PRIME-ShutterMode": "Pre-Exposure", "COM3-Handshaking": "Off", "445 AO-SupportsTriggering": "0", "ROI": "0-0-1200-1200", "COM9-Verbose": "1", "PRIME-Color": "OFF", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "PRIME-ShutterOpenDelay": "0", "COM1-Parity": "None", "Slice": 0, "SlicePosition": -50, "COM9-DelayBetweenCharsMs": "0.0000", "555-Name": "DA Shutter", "PIEZO-LudlSingleAxisName": "B", "XLIGHT Emission Wheel-Label": "679/29 (A)", "640 Shutter-Name": "AnalogIO", "640 AO-Sequenceable": "1", "520 Shutter-Demo": "No", "528 Shutter-Demo": "No", "470-Name": "DA Shutter", "520-DA Device": "520 Shutter", "XYStage-Description": "Ludl XY stage driver adapter", "445 Shutter-MinVolts": "0.0000", "528 AO-TriggerSampleRate": "100000", "405 AO-MinVolts": "0.0000", "405 Shutter-TriggerSequenceLength": "1000", "Height": 1200, "555 AO-Block voltage": "false", "640 Shutter-Volts": "5.0000", "PixelType": "GRAY16", "445 Shutter-Block voltage": "false", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "520 Shutter-TriggerSequenceLength": "1000", "COM9-Name": "COM9", "Core-SLM": "", "555 AO-Volts": "0.0000", "555 Shutter-Block voltage": "false", "XYStage-Speed": "1800.0000", "520 AO-MaxVolts": "5.0000", "528 Shutter-SupportsTriggering": "0", "COM1-Description": "Serial port driver (boost:asio)", "PRIME-MetadataEnabled": "No", "520 Shutter-MinVolts": "0.0000", "470 Shutter-Sequenceable": "1", "PRIME-PreampDelay": "0", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-MinVolts": "0.0000", "ChannelIndex": 2, "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Core-AutoShutter": "1", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "Exposure-ms": 320, "PRIME-PP 2 ENABLED": "Yes", "640 Shutter-TriggerSampleRate": "100000", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "470 Shutter-IOChannel": "Dev1/ao10", "405 AO-Demo": "No", "PixelSizeUm": 1.1, "470 AO-Demo": "No", "470 Shutter-MaxVolts": "5.0000", "Vincent-D3-Name": "Vincent-D3", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FileName": "img_000000000_Il-A_000.tif", "LudlController-Reset": "Operate", "PRIME-Exposure": "320.00", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "445-Name": "DA Shutter", "555 Shutter-Name": "AnalogIO", "520 Shutter-TriggerSampleRate": "100000", "445 Shutter-IOChannel": "Dev1/ao9", "PRIME-TransposeCorrection": "0", "XYStage-StepSize": "0.1000", "445-Description": "DA device that is used as a shutter", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 AO-IOChannel": "Dev1/ao7", "520 Shutter-Name": "AnalogIO", "COM3-DataBits": "8", "640 AO-Volts": "4.5000", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "555 Shutter-IOChannel": "Dev1/ao13", "WaitInterval": null, "520 Shutter-Sequenceable": "1", "YPositionUm": 20690.300000000003, "445-State": "0", "445-DA Device": "445 Shutter", "PRIME-CircularBufferAutoSize": "ON", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "555 AO-TriggerSampleRate": "100000", "445 Shutter-Volts": "0.0000", "XLIGHT Hub-Port": "COM3", "Core-AutoFocus": "", "COM4-Handshaking": "Off", "528 Shutter-MinVolts": "0.0000", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "445 Shutter-Name": "AnalogIO", "528 Shutter-Sequenceable": "1", "XYStage-TransposeMirrorX": "0", "XYStage-TransposeMirrorY": "0", "PRIME-ReadoutRate": "100MHz 16bit", "XYStage-Name": "Stage", "640 AO-SupportsTriggering": "0", "640-Name": "DA Shutter", "640 Shutter-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "640 AO-Block voltage": "false", "445 Shutter-Sequenceable": "1", "470 AO-MaxVolts": "5.0000", "COM4-BaudRate": "9600", "470-Description": "DA device that is used as a shutter", "520 Shutter-Description": "NI DAC", "PRIME-CCDTemperature": "-14.9600", "Time": "2019-12-17 21:58:51 -0500", "640 AO-TriggerSequenceLength": "1000", "470 AO-Name": "AnalogIO", "XLIGHT Disk slider-Label": "Disk Pos 40um", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "Binning": "1x1", "TE2000-ModelType": "1", "COM3-AnswerTimeout": "500.0000", "555 Shutter-SupportsTriggering": "0", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "TE2000-Port": "COM4", "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Description": "NI DAC", "COM4-AnswerTimeout": "500.0000", "COM1-DelayBetweenCharsMs": "0.0000", "FilterBlock-Label": "Confocal", "445 AO-Name": "AnalogIO", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-Timing-ReadoutTimeNs": "24672000.0000", "528 AO-Demo": "No", "520 AO-Sequenceable": "1", "555-Description": "DA device that is used as a shutter", "405-DA Device": "405 Shutter", "405 AO-TriggerSequenceLength": "1000", "640 AO-MinVolts": "0.0000", "640 Shutter-Block voltage": "false", "FrameIndex": 0, "PRIME-Port": "CMOS", "555 AO-MinVolts": "0.0000", "COM3-Description": "Serial port driver (boost:asio)", "528 AO-Description": "NI DAC", "555 AO-IOChannel": "Dev1/ao6", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "555 AO-TriggerInputLine": "/Dev1/PFI0", "PRIME-ActualInterval-ms": "374.3700", "LudlController-Port": "COM9", "445 Shutter-Demo": "No", "PRIME-Trigger-Expose Out-Mux": "1", "SliceIndex": 0, "PRIME-CCDTemperatureSetPoint": "-15.0000", "405 Shutter-MinVolts": "0.0000", "PRIME-ExposeOutMode": "First Row", "AxisPositions": null, "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Volts": "0.0000", "528-Name": "DA Shutter", "555-State": "0", "XLIGHT Dichroic-Label": "Di03-R635-t1", "445 AO-Sequenceable": "1", "405-Name": "DA Shutter", "405 AO-IOChannel": "Dev1/ao0", "Core-Camera": "PRIME", "Focus-Name": "Focus", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "405 Shutter-IOChannel": "Dev1/ao8", "640 AO-Demo": "No", "640 Shutter-SupportsTriggering": "0", "445 AO-Demo": "No", "555-DA Device": "555 Shutter", "470 Shutter-Description": "NI DAC", "528 AO-Volts": "0.0000", "COM1-DataBits": "8", "Vincent-D3-Address": "x", "520 AO-SupportsTriggering": "0", "405-Description": "DA device that is used as a shutter", "640 Shutter-MinVolts": "0.0000", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "405 AO-Name": "AnalogIO", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "640 AO-TriggerInputLine": "/Dev1/PFI0", "405 AO-TriggerInputLine": "/Dev1/PFI0", "640-Description": "DA device that is used as a shutter", "445 Shutter-TriggerSampleRate": "100000", "470 Shutter-TriggerSequenceLength": "1000", "COM1-StopBits": "1", "520 Shutter-SupportsTriggering": "0", "405-State": "0", "Core-Initialize": "1", "XLIGHT Emission Wheel-State": "7", "520 AO-Description": "NI DAC", "528 AO-TriggerInputLine": "/Dev1/PFI0", "445 Shutter-MaxVolts": "5.0000", "COM9-Handshaking": "Off", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "XLIGHT Spin motor-Label": "On (spinning)", "PRIME-PP 5 ENABLED": "No", "UUID": "513f2a6a-9419-41bb-b0cd-a6743ea60b05", "PRIME-TriggerMode": "Internal Trigger", "COM1-Handshaking": "Off", "PRIME-Timing-PostTriggerDelayNs": "49344000.0000", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "Focus-Position": "16479.8000", "528 Shutter-Block voltage": "false", "Channel": "Il-A", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "555 Shutter-Demo": "No", "405 Shutter-Demo": "No", "528 AO-Name": "AnalogIO", "520-State": "0", "Core-Galvo": "", "405 Shutter-TriggerSampleRate": "100000", "Focus-FocusStepSize_(nm)": "100", "PRIME-PixelType": "16bit", "COM4-DataBits": "8", "470 AO-IOChannel": "Dev1/ao2", "520 AO-MinVolts": "0.0000", "COM9-Parity": "None", "PRIME-CircularBufferEnabled": "ON", "PRIME-PP 6 Reset": "No", "640 AO-Description": "NI DAC", "Source": "PRIME", "445 AO-MinVolts": "0.0000", "PIEZO-Name": "Stage", "445 AO-Description": "NI DAC", "PRIME-CircularBufferFrameCount": "46", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "520 AO-Volts": "0.0000", "528 Shutter-TriggerSampleRate": "100000", "XPositionUm": 62321, "OpticalPath-State": "4", "PRIME-PP 3 THRESHOLD": "80", "PRIME-PVCAM Version": "3.7.1", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Gain": "1-HDR", "640 Shutter-TriggerSequenceLength": "1000", "528 AO-SupportsTriggering": "0", "445 AO-TriggerInputLine": "/Dev1/PFI0", "OpticalPath-Name": "OpticalPath", "PRIME-PP 4 ENABLED": "Yes", "PRIME-PVCAM Adapter Version": "1.3.29", "COM3-Name": "COM3", "405 AO-MaxVolts": "5.0000", "520 Shutter-Block voltage": "false", "PRIME-Timing-ClearingTimeNs": "49344000.0000", "Core-Focus": "PIEZO", "528 AO-Block voltage": "false", "TE2000-Description": "Nikon TE2000 hub", "528 AO-MinVolts": "0.0000", "COM4-Parity": "None", "Summary": { "Slices": 11, "ChContrastMin": [ 0, 0, 0, 0, 0, 0 ], "PixelAspect": 1, "SlicesFirst": true, "Interval_ms": 0, "Time": "2019-12-17 21:58:39 -0500", "Prefix": "seq_3", "InitialPositionList": [ { "Label": "Pos0", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.435], "Focus": [16480.2], "XYStage": [ 69822.40000000001, 20100.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos1", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.425], "Focus": [16480.3], "XYStage": [ 69004, 20101 ] }, "GridColumnIndex": 0 }, { "Label": "Pos2", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.44], "Focus": [16480.1], "XYStage": [ 63119.5, 20690.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos3", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.405], "Focus": [16480.2], "XYStage": [ 62321, 20690.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos4", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.415], "Focus": [16480], "XYStage": [ 56604.100000000006, 20688.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos5", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.42000000000002], "Focus": [16480.1], "XYStage": [ 56034.200000000004, 20688.800000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos6", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.455], "Focus": [16480], "XYStage": [ 50889.3, 20496.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos7", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.43], "Focus": [16480.2], "XYStage": [ 50101.600000000006, 20496.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos8", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.67000000000002], "Focus": [16479.9], "XYStage": [ 44818.600000000006, 20496.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos9", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.67000000000002], "Focus": [16480], "XYStage": [ 43897.9, 20436.800000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos10", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.675], "Focus": [16479.9], "XYStage": [ 39670.600000000006, 20664.9 ] }, "GridColumnIndex": 0 }, { "Label": "Pos11", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.69], "Focus": [16479.9], "XYStage": [ 38734, 20664.600000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos12", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.695], "Focus": [16480.1], "XYStage": [ 34037.200000000004, 20664.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos13", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.685], "Focus": [16480.1], "XYStage": [ 33152, 20664.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos14", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.69], "Focus": [16480.1], "XYStage": [ 28122.800000000003, 20664.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos15", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.675], "Focus": [16480], "XYStage": [ 27422.9, 20664.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos16", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.64000000000001], "Focus": [16480], "XYStage": [ 23005.2, 20664.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos17", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.69], "Focus": [16479.9], "XYStage": [ 21820.600000000002, 20409.600000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos18", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.41], "Focus": [16480.1], "XYStage": [ 73697.6, -13222.800000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos19", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.455], "Focus": [16480.1], "XYStage": [ 72765.8, -13223 ] }, "GridColumnIndex": 0 }, { "Label": "Pos20", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.4], "Focus": [16480.2], "XYStage": [ 67445.5, -12303.800000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos21", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.415], "Focus": [16480.2], "XYStage": [ 66613.3, -12303.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos22", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.415], "Focus": [16480.1], "XYStage": [ 61819.4, -11936.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos23", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.405], "Focus": [16480.2], "XYStage": [ 61325.700000000004, -11937.1 ] }, "GridColumnIndex": 0 }, { "Label": "Pos24", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.46], "Focus": [16480.2], "XYStage": [ 55377, -10974.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos25", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.445], "Focus": [16480.1], "XYStage": [ 54827, -11214.800000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos26", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.435], "Focus": [16480.2], "XYStage": [ 49937.100000000006, -10919.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos27", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.425], "Focus": [16480.1], "XYStage": [ 48764, -10919.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos28", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.42000000000002], "Focus": [16480.2], "XYStage": [ 43978.4, -10920.400000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos29", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.435], "Focus": [16480.2], "XYStage": [ 43106.3, -10920.400000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos30", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.405], "Focus": [16480.3], "XYStage": [ 38212.4, -10920.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos31", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.445], "Focus": [16480.2], "XYStage": [ 37254.9, -10920.400000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos32", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.44], "Focus": [16480.3], "XYStage": [ 32472.800000000003, -11294.400000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos33", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [166.95000000000002], "Focus": [16480.2], "XYStage": [ 31420.7, -11294.400000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos34", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [166.95000000000002], "Focus": [16480.2], "XYStage": [ 26881.5, -11640 ] }, "GridColumnIndex": 0 }, { "Label": "Pos35", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [166.98], "Focus": [16480.2], "XYStage": [ 25876.100000000002, -11641.1 ] }, "GridColumnIndex": 0 } ], "z-step_um": 10, "GridRow": 0, "BitDepth": 16, "Depth": 2, "Channels": 6, "Source": "Micro-Manager", "ChColors": [ -16711681, -256, -65281, -1, -1, -16776961 ], "MetadataVersion": 10, "KeepShutterOpenChannels": true, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536, 65536 ], "CustomIntervals_ms": [], "Height": 1200, "MicroManagerVersion": "1.4.23 20180219", "GridColumn": 0, "UUID": "8c8f9d20-b128-4577-9f91-89e70f22d319", "PixelType": "GRAY16", "Width": 1200, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\Yan\\20191217-YC113-S5S6", "Frames": 1, "Comment": "", "UserName": "Nikon", "PixelSize_um": 1.1, "ROI": [ 0, 0, 1200, 1200 ], "Date": "2019-12-17", "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC", "P-DAPI-high" ], "KeepShutterOpenSlices": true, "IJType": 1, "ComputerName": "ZadorConfocal2", "Positions": 36, "PositionIndex": 3, "CameraTimeout": 20000, "TimeFirst": false }, "640 AO-Name": "AnalogIO", "Vincent-D3-Command": "Close" }, "FrameKey-0-2-1": { "COM4-StopBits": "1", "528 AO-MaxVolts": "5.0000", "XLIGHT Excitation Wheel-State": "2", "470 Shutter-Name": "AnalogIO", "Core-Shutter": "640", "555 AO-Demo": "No", "XLIGHT Dichroic-State": "2", "XYStage-StepSize-Y": "0.1000", "ZPositionUm": 137.405, "XYStage-StepSize-X": "0.1000", "PRIME-FirmwareVersion": "20.28", "PRIME-ClearCycles": "2", "520-Name": "DA Shutter", "405 Shutter-Name": "AnalogIO", "PIEZO-StepSize": "0.0050", "PRIME-ClearMode": "Pre-Exposure", "470 AO-MinVolts": "0.0000", "405 AO-SupportsTriggering": "1", "CameraChannelIndex": 0, "XYStage-StartSpeed": "500.0000", "COM9-StopBits": "2", "405 AO-Description": "NI DAC", "COM4-DelayBetweenCharsMs": "0.0000", "445 AO-Block voltage": "false", "520 AO-TriggerInputLine": "/Dev1/PFI0", "555 AO-Description": "NI DAC", "520 AO-TriggerSampleRate": "100000", "COM4-Verbose": "1", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM4-Name": "COM4", "405 Shutter-Volts": "0.0000", "Vincent-D3-Port": "COM1", "PRIME-PP 4 MIN ADU AFFECTED": "200", "470 Shutter-MinVolts": "0.0000", "PRIME-PP 3 ENABLED": "Yes", "470 AO-Description": "NI DAC", "PRIME-Description": "PVCAM API device adapter", "520 Shutter-MaxVolts": "5.0000", "520 Shutter-Volts": "0.0000", "528 AO-Sequenceable": "1", "PRIME-Binning": "1x1", "555 Shutter-MaxVolts": "5.0000", "528 Shutter-TriggerSequenceLength": "1000", "405 Shutter-Block voltage": "false", "XLIGHT Spin motor-State": "1", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "Vincent-D3-Time to open (ms)": "35.0000", "Vincent-D3-Time to close (ms)": "35.0000", "445 AO-TriggerSampleRate": "100000", "640 Shutter-IOChannel": "Dev1/ao14", "555 AO-Sequenceable": "1", "520 AO-Name": "AnalogIO", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "470 AO-Block voltage": "false", "COM1-Verbose": "1", "COM9-AnswerTimeout": "10000.0000", "528 Shutter-Name": "AnalogIO", "PRIME-Trigger Timeout (secs)": "10", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "NextFrame": 0, "470 AO-TriggerSequenceLength": "1000", "PRIME-TransposeXY": "0", "445 AO-TriggerSequenceLength": "1000", "COM1-Name": "COM1", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "COM9-DataBits": "8", "COM1-AnswerTimeout": "500.0000", "Camera": "", "TE2000-Version": "V2.50-V2.50-V2.10", "528 Shutter-Volts": "0.0000", "XLIGHT Hub-Name": "XLIGHT Hub", "PIEZO-Description": "Ludl stage driver adapter", "PositionName": "Pos3", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSampleRate": "100000", "PRIME-FanSpeedSetpoint": "High", "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "405 AO-TriggerSampleRate": "100000", "470 Shutter-Block voltage": "false", "470-State": "0", "COM3-Parity": "None", "405 AO-Volts": "0.0000", "PRIME-TransposeMirrorY": "0", "PRIME-ChipName": "GS144BSI", "470 AO-Volts": "0.0000", "PRIME-TransposeMirrorX": "0", "445 Shutter-SupportsTriggering": "0", "470 Shutter-SupportsTriggering": "0", "XLIGHT Hub-Description": "XLIGHT hub", "PRIME-PreampOffLimit": "10000", "470 AO-Sequenceable": "1", "528 Shutter-MaxVolts": "5.0000", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "Core-XYStage": "XYStage", "470 AO-TriggerSampleRate": "100000", "PRIME-Timing-PreTriggerDelayNs": "24672000.0000", "528-DA Device": "528 Shutter", "Focus-Description": "Nikon TE2000 focus adapter", "FilterBlock-State": "5", "520 AO-Demo": "No", "COM3-DelayBetweenCharsMs": "0.0000", "470 Shutter-Volts": "0.0000", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "PRIME-PP 2 THRESHOLD": "125", "PRIME-PP 4 THRESHOLD": "75", "XYStage-Acceleration": "4.0000", "470 Shutter-Demo": "No", "PRIME-Name": "PMPCIECam00", "640-DA Device": "640 Shutter", "405 AO-Block voltage": "false", "ElapsedTime-ms": 145030, "PRIME-Offset": "100", "445 AO-IOChannel": "Dev1/ao1", "640 AO-MaxVolts": "5.0000", "PositionIndex": 3, "PRIME-PP 1 ENABLED": "Yes", "COM1-BaudRate": "9600", "640-State": "1", "COM3-Verbose": "1", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "LudlController-TransmissionDelay": "0", "PRIME-FullWellCapacity": "62000", "PRIME-SMARTStreamingEnabled": "No", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "640 Shutter-Description": "NI DAC", "528-Description": "DA device that is used as a shutter", "COM4-Description": "Serial port driver (boost:asio)", "405 Shutter-Description": "NI DAC", "TE2000-Name": "TE2000", "528 Shutter-Description": "NI DAC", "COM9-Description": "Serial port driver (boost:asio)", "LudlController-Name": "LudlController", "445 Shutter-TriggerSequenceLength": "1000", "PRIME-SerialNumber": "A18B203008", "445 AO-Volts": "0.0000", "PRIME-X-dimension": "1200", "PRIME-ShutterCloseDelay": "0", "XLIGHT Disk slider-State": "2", "Position": "Default", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 AO-IOChannel": "Dev1/ao5", "520 AO-IOChannel": "Dev1/ao4", "528 AO-TriggerSequenceLength": "1000", "405 Shutter-MaxVolts": "5.0000", "470-DA Device": "470 Shutter", "PRIME-FTCapable": "No", "Core-ChannelGroup": "Channels", "555 AO-SupportsTriggering": "0", "PRIME-Y-dimension": "1200", "Frame": 0, "555 Shutter-Sequenceable": "1", "PRIME-Timing-ExposureTimeNs": "9992000.0000", "Core-ImageProcessor": "", "528-State": "0", "520 AO-Block voltage": "false", "XLIGHT Excitation Wheel-Label": "QUAD", "640 Shutter-Demo": "No", "COM3-StopBits": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "520 Shutter-IOChannel": "Dev1/ao11", "Width": 1200, "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-SupportsTriggering": "0", "640 AO-TriggerSampleRate": "100000", "520-Description": "DA device that is used as a shutter", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "405 Shutter-Sequenceable": "1", "405 Shutter-SupportsTriggering": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-TriggerSampleRate": "100000", "640 Shutter-MaxVolts": "5.0000", "555 AO-MaxVolts": "5.0000", "520 AO-TriggerSequenceLength": "1000", "COM3-BaudRate": "9600", "COM9-BaudRate": "9600", "Vincent-D3-Channel #": "Ch. #1", "PRIME-PMode": "Normal", "OpticalPath-Label": "Left(100)", "BitDepth": 16, "555 AO-TriggerSequenceLength": "1000", "445 Shutter-Description": "NI DAC", "405 AO-Sequenceable": "1", "PRIME-AcquisitionMethod": "Callbacks", "555 AO-Name": "AnalogIO", "PRIME-ShutterMode": "Pre-Exposure", "COM3-Handshaking": "Off", "445 AO-SupportsTriggering": "0", "ROI": "0-0-1200-1200", "COM9-Verbose": "1", "PRIME-Color": "OFF", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "PRIME-ShutterOpenDelay": "0", "COM1-Parity": "None", "Slice": 1, "SlicePosition": -40, "COM9-DelayBetweenCharsMs": "0.0000", "555-Name": "DA Shutter", "PIEZO-LudlSingleAxisName": "B", "XLIGHT Emission Wheel-Label": "679/29 (A)", "640 Shutter-Name": "AnalogIO", "640 AO-Sequenceable": "1", "520 Shutter-Demo": "No", "528 Shutter-Demo": "No", "470-Name": "DA Shutter", "520-DA Device": "520 Shutter", "XYStage-Description": "Ludl XY stage driver adapter", "445 Shutter-MinVolts": "0.0000", "528 AO-TriggerSampleRate": "100000", "405 AO-MinVolts": "0.0000", "405 Shutter-TriggerSequenceLength": "1000", "Height": 1200, "555 AO-Block voltage": "false", "640 Shutter-Volts": "5.0000", "PixelType": "GRAY16", "445 Shutter-Block voltage": "false", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "520 Shutter-TriggerSequenceLength": "1000", "COM9-Name": "COM9", "Core-SLM": "", "555 AO-Volts": "0.0000", "555 Shutter-Block voltage": "false", "XYStage-Speed": "1800.0000", "520 AO-MaxVolts": "5.0000", "528 Shutter-SupportsTriggering": "0", "COM1-Description": "Serial port driver (boost:asio)", "PRIME-MetadataEnabled": "No", "520 Shutter-MinVolts": "0.0000", "470 Shutter-Sequenceable": "1", "PRIME-PreampDelay": "0", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-MinVolts": "0.0000", "ChannelIndex": 2, "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Core-AutoShutter": "1", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "Exposure-ms": 320, "PRIME-PP 2 ENABLED": "Yes", "640 Shutter-TriggerSampleRate": "100000", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "470 Shutter-IOChannel": "Dev1/ao10", "405 AO-Demo": "No", "PixelSizeUm": 1.1, "470 AO-Demo": "No", "470 Shutter-MaxVolts": "5.0000", "Vincent-D3-Name": "Vincent-D3", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FileName": "img_000000000_Il-A_001.tif", "LudlController-Reset": "Operate", "PRIME-Exposure": "320.00", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "445-Name": "DA Shutter", "555 Shutter-Name": "AnalogIO", "520 Shutter-TriggerSampleRate": "100000", "445 Shutter-IOChannel": "Dev1/ao9", "PRIME-TransposeCorrection": "0", "XYStage-StepSize": "0.1000", "445-Description": "DA device that is used as a shutter", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 AO-IOChannel": "Dev1/ao7", "520 Shutter-Name": "AnalogIO", "COM3-DataBits": "8", "640 AO-Volts": "4.5000", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "555 Shutter-IOChannel": "Dev1/ao13", "WaitInterval": null, "520 Shutter-Sequenceable": "1", "YPositionUm": 20690.300000000003, "445-State": "0", "445-DA Device": "445 Shutter", "PRIME-CircularBufferAutoSize": "ON", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "555 AO-TriggerSampleRate": "100000", "445 Shutter-Volts": "0.0000", "XLIGHT Hub-Port": "COM3", "Core-AutoFocus": "", "COM4-Handshaking": "Off", "528 Shutter-MinVolts": "0.0000", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "445 Shutter-Name": "AnalogIO", "528 Shutter-Sequenceable": "1", "XYStage-TransposeMirrorX": "0", "XYStage-TransposeMirrorY": "0", "PRIME-ReadoutRate": "100MHz 16bit", "XYStage-Name": "Stage", "640 AO-SupportsTriggering": "0", "640-Name": "DA Shutter", "640 Shutter-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "640 AO-Block voltage": "false", "445 Shutter-Sequenceable": "1", "470 AO-MaxVolts": "5.0000", "COM4-BaudRate": "9600", "470-Description": "DA device that is used as a shutter", "520 Shutter-Description": "NI DAC", "PRIME-CCDTemperature": "-14.9600", "Time": "2019-12-17 21:58:52 -0500", "640 AO-TriggerSequenceLength": "1000", "470 AO-Name": "AnalogIO", "XLIGHT Disk slider-Label": "Disk Pos 40um", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "Binning": "1x1", "TE2000-ModelType": "1", "COM3-AnswerTimeout": "500.0000", "555 Shutter-SupportsTriggering": "0", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "TE2000-Port": "COM4", "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Description": "NI DAC", "COM4-AnswerTimeout": "500.0000", "COM1-DelayBetweenCharsMs": "0.0000", "FilterBlock-Label": "Confocal", "445 AO-Name": "AnalogIO", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-Timing-ReadoutTimeNs": "24672000.0000", "528 AO-Demo": "No", "520 AO-Sequenceable": "1", "555-Description": "DA device that is used as a shutter", "405-DA Device": "405 Shutter", "405 AO-TriggerSequenceLength": "1000", "640 AO-MinVolts": "0.0000", "640 Shutter-Block voltage": "false", "FrameIndex": 0, "PRIME-Port": "CMOS", "555 AO-MinVolts": "0.0000", "COM3-Description": "Serial port driver (boost:asio)", "528 AO-Description": "NI DAC", "555 AO-IOChannel": "Dev1/ao6", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "555 AO-TriggerInputLine": "/Dev1/PFI0", "PRIME-ActualInterval-ms": "374.3700", "LudlController-Port": "COM9", "445 Shutter-Demo": "No", "PRIME-Trigger-Expose Out-Mux": "1", "SliceIndex": 1, "PRIME-CCDTemperatureSetPoint": "-15.0000", "405 Shutter-MinVolts": "0.0000", "PRIME-ExposeOutMode": "First Row", "AxisPositions": null, "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Volts": "0.0000", "528-Name": "DA Shutter", "555-State": "0", "XLIGHT Dichroic-Label": "Di03-R635-t1", "445 AO-Sequenceable": "1", "405-Name": "DA Shutter", "405 AO-IOChannel": "Dev1/ao0", "Core-Camera": "PRIME", "Focus-Name": "Focus", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "405 Shutter-IOChannel": "Dev1/ao8", "640 AO-Demo": "No", "640 Shutter-SupportsTriggering": "0", "445 AO-Demo": "No", "555-DA Device": "555 Shutter", "470 Shutter-Description": "NI DAC", "528 AO-Volts": "0.0000", "COM1-DataBits": "8", "Vincent-D3-Address": "x", "520 AO-SupportsTriggering": "0", "405-Description": "DA device that is used as a shutter", "640 Shutter-MinVolts": "0.0000", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "405 AO-Name": "AnalogIO", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "640 AO-TriggerInputLine": "/Dev1/PFI0", "405 AO-TriggerInputLine": "/Dev1/PFI0", "640-Description": "DA device that is used as a shutter", "445 Shutter-TriggerSampleRate": "100000", "470 Shutter-TriggerSequenceLength": "1000", "COM1-StopBits": "1", "520 Shutter-SupportsTriggering": "0", "405-State": "0", "Core-Initialize": "1", "XLIGHT Emission Wheel-State": "7", "520 AO-Description": "NI DAC", "528 AO-TriggerInputLine": "/Dev1/PFI0", "445 Shutter-MaxVolts": "5.0000", "COM9-Handshaking": "Off", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "XLIGHT Spin motor-Label": "On (spinning)", "PRIME-PP 5 ENABLED": "No", "UUID": "b6d84e25-6516-4b60-95e1-8d9a00e594c7", "PRIME-TriggerMode": "Internal Trigger", "COM1-Handshaking": "Off", "PRIME-Timing-PostTriggerDelayNs": "49344000.0000", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "Focus-Position": "16479.8000", "528 Shutter-Block voltage": "false", "Channel": "Il-A", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "555 Shutter-Demo": "No", "405 Shutter-Demo": "No", "528 AO-Name": "AnalogIO", "520-State": "0", "Core-Galvo": "", "405 Shutter-TriggerSampleRate": "100000", "Focus-FocusStepSize_(nm)": "100", "PRIME-PixelType": "16bit", "COM4-DataBits": "8", "470 AO-IOChannel": "Dev1/ao2", "520 AO-MinVolts": "0.0000", "COM9-Parity": "None", "PRIME-CircularBufferEnabled": "ON", "PRIME-PP 6 Reset": "No", "640 AO-Description": "NI DAC", "Source": "PRIME", "445 AO-MinVolts": "0.0000", "PIEZO-Name": "Stage", "445 AO-Description": "NI DAC", "PRIME-CircularBufferFrameCount": "46", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "520 AO-Volts": "0.0000", "528 Shutter-TriggerSampleRate": "100000", "XPositionUm": 62321, "OpticalPath-State": "4", "PRIME-PP 3 THRESHOLD": "80", "PRIME-PVCAM Version": "3.7.1", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Gain": "1-HDR", "640 Shutter-TriggerSequenceLength": "1000", "528 AO-SupportsTriggering": "0", "445 AO-TriggerInputLine": "/Dev1/PFI0", "OpticalPath-Name": "OpticalPath", "PRIME-PP 4 ENABLED": "Yes", "PRIME-PVCAM Adapter Version": "1.3.29", "COM3-Name": "COM3", "405 AO-MaxVolts": "5.0000", "520 Shutter-Block voltage": "false", "PRIME-Timing-ClearingTimeNs": "49344000.0000", "Core-Focus": "PIEZO", "528 AO-Block voltage": "false", "TE2000-Description": "Nikon TE2000 hub", "528 AO-MinVolts": "0.0000", "COM4-Parity": "None", "Summary": { "Slices": 11, "ChContrastMin": [ 0, 0, 0, 0, 0, 0 ], "PixelAspect": 1, "SlicesFirst": true, "Interval_ms": 0, "Time": "2019-12-17 21:58:39 -0500", "Prefix": "seq_3", "InitialPositionList": [ { "Label": "Pos0", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.435], "Focus": [16480.2], "XYStage": [ 69822.40000000001, 20100.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos1", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.425], "Focus": [16480.3], "XYStage": [ 69004, 20101 ] }, "GridColumnIndex": 0 }, { "Label": "Pos2", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.44], "Focus": [16480.1], "XYStage": [ 63119.5, 20690.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos3", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.405], "Focus": [16480.2], "XYStage": [ 62321, 20690.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos4", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.415], "Focus": [16480], "XYStage": [ 56604.100000000006, 20688.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos5", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.42000000000002], "Focus": [16480.1], "XYStage": [ 56034.200000000004, 20688.800000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos6", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.455], "Focus": [16480], "XYStage": [ 50889.3, 20496.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos7", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.43], "Focus": [16480.2], "XYStage": [ 50101.600000000006, 20496.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos8", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.67000000000002], "Focus": [16479.9], "XYStage": [ 44818.600000000006, 20496.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos9", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.67000000000002], "Focus": [16480], "XYStage": [ 43897.9, 20436.800000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos10", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.675], "Focus": [16479.9], "XYStage": [ 39670.600000000006, 20664.9 ] }, "GridColumnIndex": 0 }, { "Label": "Pos11", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.69], "Focus": [16479.9], "XYStage": [ 38734, 20664.600000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos12", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.695], "Focus": [16480.1], "XYStage": [ 34037.200000000004, 20664.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos13", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.685], "Focus": [16480.1], "XYStage": [ 33152, 20664.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos14", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.69], "Focus": [16480.1], "XYStage": [ 28122.800000000003, 20664.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos15", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.675], "Focus": [16480], "XYStage": [ 27422.9, 20664.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos16", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.64000000000001], "Focus": [16480], "XYStage": [ 23005.2, 20664.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos17", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.69], "Focus": [16479.9], "XYStage": [ 21820.600000000002, 20409.600000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos18", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.41], "Focus": [16480.1], "XYStage": [ 73697.6, -13222.800000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos19", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.455], "Focus": [16480.1], "XYStage": [ 72765.8, -13223 ] }, "GridColumnIndex": 0 }, { "Label": "Pos20", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.4], "Focus": [16480.2], "XYStage": [ 67445.5, -12303.800000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos21", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.415], "Focus": [16480.2], "XYStage": [ 66613.3, -12303.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos22", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.415], "Focus": [16480.1], "XYStage": [ 61819.4, -11936.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos23", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.405], "Focus": [16480.2], "XYStage": [ 61325.700000000004, -11937.1 ] }, "GridColumnIndex": 0 }, { "Label": "Pos24", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.46], "Focus": [16480.2], "XYStage": [ 55377, -10974.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos25", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.445], "Focus": [16480.1], "XYStage": [ 54827, -11214.800000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos26", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.435], "Focus": [16480.2], "XYStage": [ 49937.100000000006, -10919.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos27", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.425], "Focus": [16480.1], "XYStage": [ 48764, -10919.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos28", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.42000000000002], "Focus": [16480.2], "XYStage": [ 43978.4, -10920.400000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos29", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.435], "Focus": [16480.2], "XYStage": [ 43106.3, -10920.400000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos30", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.405], "Focus": [16480.3], "XYStage": [ 38212.4, -10920.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos31", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.445], "Focus": [16480.2], "XYStage": [ 37254.9, -10920.400000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos32", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.44], "Focus": [16480.3], "XYStage": [ 32472.800000000003, -11294.400000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos33", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [166.95000000000002], "Focus": [16480.2], "XYStage": [ 31420.7, -11294.400000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos34", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [166.95000000000002], "Focus": [16480.2], "XYStage": [ 26881.5, -11640 ] }, "GridColumnIndex": 0 }, { "Label": "Pos35", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [166.98], "Focus": [16480.2], "XYStage": [ 25876.100000000002, -11641.1 ] }, "GridColumnIndex": 0 } ], "z-step_um": 10, "GridRow": 0, "BitDepth": 16, "Depth": 2, "Channels": 6, "Source": "Micro-Manager", "ChColors": [ -16711681, -256, -65281, -1, -1, -16776961 ], "MetadataVersion": 10, "KeepShutterOpenChannels": true, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536, 65536 ], "CustomIntervals_ms": [], "Height": 1200, "MicroManagerVersion": "1.4.23 20180219", "GridColumn": 0, "UUID": "8c8f9d20-b128-4577-9f91-89e70f22d319", "PixelType": "GRAY16", "Width": 1200, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\Yan\\20191217-YC113-S5S6", "Frames": 1, "Comment": "", "UserName": "Nikon", "PixelSize_um": 1.1, "ROI": [ 0, 0, 1200, 1200 ], "Date": "2019-12-17", "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC", "P-DAPI-high" ], "KeepShutterOpenSlices": true, "IJType": 1, "ComputerName": "ZadorConfocal2", "Positions": 36, "PositionIndex": 3, "CameraTimeout": 20000, "TimeFirst": false }, "640 AO-Name": "AnalogIO", "Vincent-D3-Command": "Close" }, "FrameKey-0-2-2": { "COM4-StopBits": "1", "528 AO-MaxVolts": "5.0000", "XLIGHT Excitation Wheel-State": "2", "470 Shutter-Name": "AnalogIO", "Core-Shutter": "640", "555 AO-Demo": "No", "XLIGHT Dichroic-State": "2", "XYStage-StepSize-Y": "0.1000", "ZPositionUm": 147.405, "XYStage-StepSize-X": "0.1000", "PRIME-FirmwareVersion": "20.28", "PRIME-ClearCycles": "2", "520-Name": "DA Shutter", "405 Shutter-Name": "AnalogIO", "PIEZO-StepSize": "0.0050", "PRIME-ClearMode": "Pre-Exposure", "470 AO-MinVolts": "0.0000", "405 AO-SupportsTriggering": "1", "CameraChannelIndex": 0, "XYStage-StartSpeed": "500.0000", "COM9-StopBits": "2", "405 AO-Description": "NI DAC", "COM4-DelayBetweenCharsMs": "0.0000", "445 AO-Block voltage": "false", "520 AO-TriggerInputLine": "/Dev1/PFI0", "555 AO-Description": "NI DAC", "520 AO-TriggerSampleRate": "100000", "COM4-Verbose": "1", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM4-Name": "COM4", "405 Shutter-Volts": "0.0000", "Vincent-D3-Port": "COM1", "PRIME-PP 4 MIN ADU AFFECTED": "200", "470 Shutter-MinVolts": "0.0000", "PRIME-PP 3 ENABLED": "Yes", "470 AO-Description": "NI DAC", "PRIME-Description": "PVCAM API device adapter", "520 Shutter-MaxVolts": "5.0000", "520 Shutter-Volts": "0.0000", "528 AO-Sequenceable": "1", "PRIME-Binning": "1x1", "555 Shutter-MaxVolts": "5.0000", "528 Shutter-TriggerSequenceLength": "1000", "405 Shutter-Block voltage": "false", "XLIGHT Spin motor-State": "1", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "Vincent-D3-Time to open (ms)": "35.0000", "Vincent-D3-Time to close (ms)": "35.0000", "445 AO-TriggerSampleRate": "100000", "640 Shutter-IOChannel": "Dev1/ao14", "555 AO-Sequenceable": "1", "520 AO-Name": "AnalogIO", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "470 AO-Block voltage": "false", "COM1-Verbose": "1", "COM9-AnswerTimeout": "10000.0000", "528 Shutter-Name": "AnalogIO", "PRIME-Trigger Timeout (secs)": "10", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "NextFrame": 0, "470 AO-TriggerSequenceLength": "1000", "PRIME-TransposeXY": "0", "445 AO-TriggerSequenceLength": "1000", "COM1-Name": "COM1", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "COM9-DataBits": "8", "COM1-AnswerTimeout": "500.0000", "Camera": "", "TE2000-Version": "V2.50-V2.50-V2.10", "528 Shutter-Volts": "0.0000", "XLIGHT Hub-Name": "XLIGHT Hub", "PIEZO-Description": "Ludl stage driver adapter", "PositionName": "Pos3", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSampleRate": "100000", "PRIME-FanSpeedSetpoint": "High", "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "405 AO-TriggerSampleRate": "100000", "470 Shutter-Block voltage": "false", "470-State": "0", "COM3-Parity": "None", "405 AO-Volts": "0.0000", "PRIME-TransposeMirrorY": "0", "PRIME-ChipName": "GS144BSI", "470 AO-Volts": "0.0000", "PRIME-TransposeMirrorX": "0", "445 Shutter-SupportsTriggering": "0", "470 Shutter-SupportsTriggering": "0", "XLIGHT Hub-Description": "XLIGHT hub", "PRIME-PreampOffLimit": "10000", "470 AO-Sequenceable": "1", "528 Shutter-MaxVolts": "5.0000", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "Core-XYStage": "XYStage", "470 AO-TriggerSampleRate": "100000", "PRIME-Timing-PreTriggerDelayNs": "24672000.0000", "528-DA Device": "528 Shutter", "Focus-Description": "Nikon TE2000 focus adapter", "FilterBlock-State": "5", "520 AO-Demo": "No", "COM3-DelayBetweenCharsMs": "0.0000", "470 Shutter-Volts": "0.0000", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "PRIME-PP 2 THRESHOLD": "125", "PRIME-PP 4 THRESHOLD": "75", "XYStage-Acceleration": "4.0000", "470 Shutter-Demo": "No", "PRIME-Name": "PMPCIECam00", "640-DA Device": "640 Shutter", "405 AO-Block voltage": "false", "ElapsedTime-ms": 145585, "PRIME-Offset": "100", "445 AO-IOChannel": "Dev1/ao1", "640 AO-MaxVolts": "5.0000", "PositionIndex": 3, "PRIME-PP 1 ENABLED": "Yes", "COM1-BaudRate": "9600", "640-State": "1", "COM3-Verbose": "1", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "LudlController-TransmissionDelay": "0", "PRIME-FullWellCapacity": "62000", "PRIME-SMARTStreamingEnabled": "No", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "640 Shutter-Description": "NI DAC", "528-Description": "DA device that is used as a shutter", "COM4-Description": "Serial port driver (boost:asio)", "405 Shutter-Description": "NI DAC", "TE2000-Name": "TE2000", "528 Shutter-Description": "NI DAC", "COM9-Description": "Serial port driver (boost:asio)", "LudlController-Name": "LudlController", "445 Shutter-TriggerSequenceLength": "1000", "PRIME-SerialNumber": "A18B203008", "445 AO-Volts": "0.0000", "PRIME-X-dimension": "1200", "PRIME-ShutterCloseDelay": "0", "XLIGHT Disk slider-State": "2", "Position": "Default", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 AO-IOChannel": "Dev1/ao5", "520 AO-IOChannel": "Dev1/ao4", "528 AO-TriggerSequenceLength": "1000", "405 Shutter-MaxVolts": "5.0000", "470-DA Device": "470 Shutter", "PRIME-FTCapable": "No", "Core-ChannelGroup": "Channels", "555 AO-SupportsTriggering": "0", "PRIME-Y-dimension": "1200", "Frame": 0, "555 Shutter-Sequenceable": "1", "PRIME-Timing-ExposureTimeNs": "9992000.0000", "Core-ImageProcessor": "", "528-State": "0", "520 AO-Block voltage": "false", "XLIGHT Excitation Wheel-Label": "QUAD", "640 Shutter-Demo": "No", "COM3-StopBits": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "520 Shutter-IOChannel": "Dev1/ao11", "Width": 1200, "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-SupportsTriggering": "0", "640 AO-TriggerSampleRate": "100000", "520-Description": "DA device that is used as a shutter", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "405 Shutter-Sequenceable": "1", "405 Shutter-SupportsTriggering": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-TriggerSampleRate": "100000", "640 Shutter-MaxVolts": "5.0000", "555 AO-MaxVolts": "5.0000", "520 AO-TriggerSequenceLength": "1000", "COM3-BaudRate": "9600", "COM9-BaudRate": "9600", "Vincent-D3-Channel #": "Ch. #1", "PRIME-PMode": "Normal", "OpticalPath-Label": "Left(100)", "BitDepth": 16, "555 AO-TriggerSequenceLength": "1000", "445 Shutter-Description": "NI DAC", "405 AO-Sequenceable": "1", "PRIME-AcquisitionMethod": "Callbacks", "555 AO-Name": "AnalogIO", "PRIME-ShutterMode": "Pre-Exposure", "COM3-Handshaking": "Off", "445 AO-SupportsTriggering": "0", "ROI": "0-0-1200-1200", "COM9-Verbose": "1", "PRIME-Color": "OFF", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "PRIME-ShutterOpenDelay": "0", "COM1-Parity": "None", "Slice": 2, "SlicePosition": -30, "COM9-DelayBetweenCharsMs": "0.0000", "555-Name": "DA Shutter", "PIEZO-LudlSingleAxisName": "B", "XLIGHT Emission Wheel-Label": "679/29 (A)", "640 Shutter-Name": "AnalogIO", "640 AO-Sequenceable": "1", "520 Shutter-Demo": "No", "528 Shutter-Demo": "No", "470-Name": "DA Shutter", "520-DA Device": "520 Shutter", "XYStage-Description": "Ludl XY stage driver adapter", "445 Shutter-MinVolts": "0.0000", "528 AO-TriggerSampleRate": "100000", "405 AO-MinVolts": "0.0000", "405 Shutter-TriggerSequenceLength": "1000", "Height": 1200, "555 AO-Block voltage": "false", "640 Shutter-Volts": "5.0000", "PixelType": "GRAY16", "445 Shutter-Block voltage": "false", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "520 Shutter-TriggerSequenceLength": "1000", "COM9-Name": "COM9", "Core-SLM": "", "555 AO-Volts": "0.0000", "555 Shutter-Block voltage": "false", "XYStage-Speed": "1800.0000", "520 AO-MaxVolts": "5.0000", "528 Shutter-SupportsTriggering": "0", "COM1-Description": "Serial port driver (boost:asio)", "PRIME-MetadataEnabled": "No", "520 Shutter-MinVolts": "0.0000", "470 Shutter-Sequenceable": "1", "PRIME-PreampDelay": "0", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-MinVolts": "0.0000", "ChannelIndex": 2, "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Core-AutoShutter": "1", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "Exposure-ms": 320, "PRIME-PP 2 ENABLED": "Yes", "640 Shutter-TriggerSampleRate": "100000", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "470 Shutter-IOChannel": "Dev1/ao10", "405 AO-Demo": "No", "PixelSizeUm": 1.1, "470 AO-Demo": "No", "470 Shutter-MaxVolts": "5.0000", "Vincent-D3-Name": "Vincent-D3", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FileName": "img_000000000_Il-A_002.tif", "LudlController-Reset": "Operate", "PRIME-Exposure": "320.00", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "445-Name": "DA Shutter", "555 Shutter-Name": "AnalogIO", "520 Shutter-TriggerSampleRate": "100000", "445 Shutter-IOChannel": "Dev1/ao9", "PRIME-TransposeCorrection": "0", "XYStage-StepSize": "0.1000", "445-Description": "DA device that is used as a shutter", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 AO-IOChannel": "Dev1/ao7", "520 Shutter-Name": "AnalogIO", "COM3-DataBits": "8", "640 AO-Volts": "4.5000", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "555 Shutter-IOChannel": "Dev1/ao13", "WaitInterval": null, "520 Shutter-Sequenceable": "1", "YPositionUm": 20690.300000000003, "445-State": "0", "445-DA Device": "445 Shutter", "PRIME-CircularBufferAutoSize": "ON", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "555 AO-TriggerSampleRate": "100000", "445 Shutter-Volts": "0.0000", "XLIGHT Hub-Port": "COM3", "Core-AutoFocus": "", "COM4-Handshaking": "Off", "528 Shutter-MinVolts": "0.0000", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "445 Shutter-Name": "AnalogIO", "528 Shutter-Sequenceable": "1", "XYStage-TransposeMirrorX": "0", "XYStage-TransposeMirrorY": "0", "PRIME-ReadoutRate": "100MHz 16bit", "XYStage-Name": "Stage", "640 AO-SupportsTriggering": "0", "640-Name": "DA Shutter", "640 Shutter-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "640 AO-Block voltage": "false", "445 Shutter-Sequenceable": "1", "470 AO-MaxVolts": "5.0000", "COM4-BaudRate": "9600", "470-Description": "DA device that is used as a shutter", "520 Shutter-Description": "NI DAC", "PRIME-CCDTemperature": "-14.9600", "Time": "2019-12-17 21:58:53 -0500", "640 AO-TriggerSequenceLength": "1000", "470 AO-Name": "AnalogIO", "XLIGHT Disk slider-Label": "Disk Pos 40um", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "Binning": "1x1", "TE2000-ModelType": "1", "COM3-AnswerTimeout": "500.0000", "555 Shutter-SupportsTriggering": "0", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "TE2000-Port": "COM4", "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Description": "NI DAC", "COM4-AnswerTimeout": "500.0000", "COM1-DelayBetweenCharsMs": "0.0000", "FilterBlock-Label": "Confocal", "445 AO-Name": "AnalogIO", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-Timing-ReadoutTimeNs": "24672000.0000", "528 AO-Demo": "No", "520 AO-Sequenceable": "1", "555-Description": "DA device that is used as a shutter", "405-DA Device": "405 Shutter", "405 AO-TriggerSequenceLength": "1000", "640 AO-MinVolts": "0.0000", "640 Shutter-Block voltage": "false", "FrameIndex": 0, "PRIME-Port": "CMOS", "555 AO-MinVolts": "0.0000", "COM3-Description": "Serial port driver (boost:asio)", "528 AO-Description": "NI DAC", "555 AO-IOChannel": "Dev1/ao6", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "555 AO-TriggerInputLine": "/Dev1/PFI0", "PRIME-ActualInterval-ms": "374.3700", "LudlController-Port": "COM9", "445 Shutter-Demo": "No", "PRIME-Trigger-Expose Out-Mux": "1", "SliceIndex": 2, "PRIME-CCDTemperatureSetPoint": "-15.0000", "405 Shutter-MinVolts": "0.0000", "PRIME-ExposeOutMode": "First Row", "AxisPositions": null, "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Volts": "0.0000", "528-Name": "DA Shutter", "555-State": "0", "XLIGHT Dichroic-Label": "Di03-R635-t1", "445 AO-Sequenceable": "1", "405-Name": "DA Shutter", "405 AO-IOChannel": "Dev1/ao0", "Core-Camera": "PRIME", "Focus-Name": "Focus", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "405 Shutter-IOChannel": "Dev1/ao8", "640 AO-Demo": "No", "640 Shutter-SupportsTriggering": "0", "445 AO-Demo": "No", "555-DA Device": "555 Shutter", "470 Shutter-Description": "NI DAC", "528 AO-Volts": "0.0000", "COM1-DataBits": "8", "Vincent-D3-Address": "x", "520 AO-SupportsTriggering": "0", "405-Description": "DA device that is used as a shutter", "640 Shutter-MinVolts": "0.0000", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "405 AO-Name": "AnalogIO", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "640 AO-TriggerInputLine": "/Dev1/PFI0", "405 AO-TriggerInputLine": "/Dev1/PFI0", "640-Description": "DA device that is used as a shutter", "445 Shutter-TriggerSampleRate": "100000", "470 Shutter-TriggerSequenceLength": "1000", "COM1-StopBits": "1", "520 Shutter-SupportsTriggering": "0", "405-State": "0", "Core-Initialize": "1", "XLIGHT Emission Wheel-State": "7", "520 AO-Description": "NI DAC", "528 AO-TriggerInputLine": "/Dev1/PFI0", "445 Shutter-MaxVolts": "5.0000", "COM9-Handshaking": "Off", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "XLIGHT Spin motor-Label": "On (spinning)", "PRIME-PP 5 ENABLED": "No", "UUID": "8349c540-ffe0-4d83-a562-94b24bbd0c23", "PRIME-TriggerMode": "Internal Trigger", "COM1-Handshaking": "Off", "PRIME-Timing-PostTriggerDelayNs": "49344000.0000", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "Focus-Position": "16479.8000", "528 Shutter-Block voltage": "false", "Channel": "Il-A", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "555 Shutter-Demo": "No", "405 Shutter-Demo": "No", "528 AO-Name": "AnalogIO", "520-State": "0", "Core-Galvo": "", "405 Shutter-TriggerSampleRate": "100000", "Focus-FocusStepSize_(nm)": "100", "PRIME-PixelType": "16bit", "COM4-DataBits": "8", "470 AO-IOChannel": "Dev1/ao2", "520 AO-MinVolts": "0.0000", "COM9-Parity": "None", "PRIME-CircularBufferEnabled": "ON", "PRIME-PP 6 Reset": "No", "640 AO-Description": "NI DAC", "Source": "PRIME", "445 AO-MinVolts": "0.0000", "PIEZO-Name": "Stage", "445 AO-Description": "NI DAC", "PRIME-CircularBufferFrameCount": "46", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "520 AO-Volts": "0.0000", "528 Shutter-TriggerSampleRate": "100000", "XPositionUm": 62321, "OpticalPath-State": "4", "PRIME-PP 3 THRESHOLD": "80", "PRIME-PVCAM Version": "3.7.1", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Gain": "1-HDR", "640 Shutter-TriggerSequenceLength": "1000", "528 AO-SupportsTriggering": "0", "445 AO-TriggerInputLine": "/Dev1/PFI0", "OpticalPath-Name": "OpticalPath", "PRIME-PP 4 ENABLED": "Yes", "PRIME-PVCAM Adapter Version": "1.3.29", "COM3-Name": "COM3", "405 AO-MaxVolts": "5.0000", "520 Shutter-Block voltage": "false", "PRIME-Timing-ClearingTimeNs": "49344000.0000", "Core-Focus": "PIEZO", "528 AO-Block voltage": "false", "TE2000-Description": "Nikon TE2000 hub", "528 AO-MinVolts": "0.0000", "COM4-Parity": "None", "Summary": { "Slices": 11, "ChContrastMin": [ 0, 0, 0, 0, 0, 0 ], "PixelAspect": 1, "SlicesFirst": true, "Interval_ms": 0, "Time": "2019-12-17 21:58:39 -0500", "Prefix": "seq_3", "InitialPositionList": [ { "Label": "Pos0", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.435], "Focus": [16480.2], "XYStage": [ 69822.40000000001, 20100.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos1", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.425], "Focus": [16480.3], "XYStage": [ 69004, 20101 ] }, "GridColumnIndex": 0 }, { "Label": "Pos2", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.44], "Focus": [16480.1], "XYStage": [ 63119.5, 20690.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos3", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.405], "Focus": [16480.2], "XYStage": [ 62321, 20690.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos4", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.415], "Focus": [16480], "XYStage": [ 56604.100000000006, 20688.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos5", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.42000000000002], "Focus": [16480.1], "XYStage": [ 56034.200000000004, 20688.800000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos6", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.455], "Focus": [16480], "XYStage": [ 50889.3, 20496.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos7", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.43], "Focus": [16480.2], "XYStage": [ 50101.600000000006, 20496.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos8", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.67000000000002], "Focus": [16479.9], "XYStage": [ 44818.600000000006, 20496.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos9", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.67000000000002], "Focus": [16480], "XYStage": [ 43897.9, 20436.800000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos10", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.675], "Focus": [16479.9], "XYStage": [ 39670.600000000006, 20664.9 ] }, "GridColumnIndex": 0 }, { "Label": "Pos11", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.69], "Focus": [16479.9], "XYStage": [ 38734, 20664.600000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos12", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.695], "Focus": [16480.1], "XYStage": [ 34037.200000000004, 20664.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos13", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.685], "Focus": [16480.1], "XYStage": [ 33152, 20664.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos14", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.69], "Focus": [16480.1], "XYStage": [ 28122.800000000003, 20664.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos15", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.675], "Focus": [16480], "XYStage": [ 27422.9, 20664.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos16", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.64000000000001], "Focus": [16480], "XYStage": [ 23005.2, 20664.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos17", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.69], "Focus": [16479.9], "XYStage": [ 21820.600000000002, 20409.600000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos18", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.41], "Focus": [16480.1], "XYStage": [ 73697.6, -13222.800000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos19", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.455], "Focus": [16480.1], "XYStage": [ 72765.8, -13223 ] }, "GridColumnIndex": 0 }, { "Label": "Pos20", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.4], "Focus": [16480.2], "XYStage": [ 67445.5, -12303.800000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos21", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.415], "Focus": [16480.2], "XYStage": [ 66613.3, -12303.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos22", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.415], "Focus": [16480.1], "XYStage": [ 61819.4, -11936.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos23", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.405], "Focus": [16480.2], "XYStage": [ 61325.700000000004, -11937.1 ] }, "GridColumnIndex": 0 }, { "Label": "Pos24", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.46], "Focus": [16480.2], "XYStage": [ 55377, -10974.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos25", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.445], "Focus": [16480.1], "XYStage": [ 54827, -11214.800000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos26", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.435], "Focus": [16480.2], "XYStage": [ 49937.100000000006, -10919.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos27", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.425], "Focus": [16480.1], "XYStage": [ 48764, -10919.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos28", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.42000000000002], "Focus": [16480.2], "XYStage": [ 43978.4, -10920.400000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos29", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.435], "Focus": [16480.2], "XYStage": [ 43106.3, -10920.400000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos30", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.405], "Focus": [16480.3], "XYStage": [ 38212.4, -10920.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos31", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.445], "Focus": [16480.2], "XYStage": [ 37254.9, -10920.400000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos32", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.44], "Focus": [16480.3], "XYStage": [ 32472.800000000003, -11294.400000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos33", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [166.95000000000002], "Focus": [16480.2], "XYStage": [ 31420.7, -11294.400000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos34", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [166.95000000000002], "Focus": [16480.2], "XYStage": [ 26881.5, -11640 ] }, "GridColumnIndex": 0 }, { "Label": "Pos35", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [166.98], "Focus": [16480.2], "XYStage": [ 25876.100000000002, -11641.1 ] }, "GridColumnIndex": 0 } ], "z-step_um": 10, "GridRow": 0, "BitDepth": 16, "Depth": 2, "Channels": 6, "Source": "Micro-Manager", "ChColors": [ -16711681, -256, -65281, -1, -1, -16776961 ], "MetadataVersion": 10, "KeepShutterOpenChannels": true, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536, 65536 ], "CustomIntervals_ms": [], "Height": 1200, "MicroManagerVersion": "1.4.23 20180219", "GridColumn": 0, "UUID": "8c8f9d20-b128-4577-9f91-89e70f22d319", "PixelType": "GRAY16", "Width": 1200, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\Yan\\20191217-YC113-S5S6", "Frames": 1, "Comment": "", "UserName": "Nikon", "PixelSize_um": 1.1, "ROI": [ 0, 0, 1200, 1200 ], "Date": "2019-12-17", "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC", "P-DAPI-high" ], "KeepShutterOpenSlices": true, "IJType": 1, "ComputerName": "ZadorConfocal2", "Positions": 36, "PositionIndex": 3, "CameraTimeout": 20000, "TimeFirst": false }, "640 AO-Name": "AnalogIO", "Vincent-D3-Command": "Close" }, "FrameKey-0-2-3": { "COM4-StopBits": "1", "528 AO-MaxVolts": "5.0000", "XLIGHT Excitation Wheel-State": "2", "470 Shutter-Name": "AnalogIO", "Core-Shutter": "640", "555 AO-Demo": "No", "XLIGHT Dichroic-State": "2", "XYStage-StepSize-Y": "0.1000", "ZPositionUm": 157.405, "XYStage-StepSize-X": "0.1000", "PRIME-FirmwareVersion": "20.28", "PRIME-ClearCycles": "2", "520-Name": "DA Shutter", "405 Shutter-Name": "AnalogIO", "PIEZO-StepSize": "0.0050", "PRIME-ClearMode": "Pre-Exposure", "470 AO-MinVolts": "0.0000", "405 AO-SupportsTriggering": "1", "CameraChannelIndex": 0, "XYStage-StartSpeed": "500.0000", "COM9-StopBits": "2", "405 AO-Description": "NI DAC", "COM4-DelayBetweenCharsMs": "0.0000", "445 AO-Block voltage": "false", "520 AO-TriggerInputLine": "/Dev1/PFI0", "555 AO-Description": "NI DAC", "520 AO-TriggerSampleRate": "100000", "COM4-Verbose": "1", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM4-Name": "COM4", "405 Shutter-Volts": "0.0000", "Vincent-D3-Port": "COM1", "PRIME-PP 4 MIN ADU AFFECTED": "200", "470 Shutter-MinVolts": "0.0000", "PRIME-PP 3 ENABLED": "Yes", "470 AO-Description": "NI DAC", "PRIME-Description": "PVCAM API device adapter", "520 Shutter-MaxVolts": "5.0000", "520 Shutter-Volts": "0.0000", "528 AO-Sequenceable": "1", "PRIME-Binning": "1x1", "555 Shutter-MaxVolts": "5.0000", "528 Shutter-TriggerSequenceLength": "1000", "405 Shutter-Block voltage": "false", "XLIGHT Spin motor-State": "1", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "Vincent-D3-Time to open (ms)": "35.0000", "Vincent-D3-Time to close (ms)": "35.0000", "445 AO-TriggerSampleRate": "100000", "640 Shutter-IOChannel": "Dev1/ao14", "555 AO-Sequenceable": "1", "520 AO-Name": "AnalogIO", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "470 AO-Block voltage": "false", "COM1-Verbose": "1", "COM9-AnswerTimeout": "10000.0000", "528 Shutter-Name": "AnalogIO", "PRIME-Trigger Timeout (secs)": "10", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "NextFrame": 0, "470 AO-TriggerSequenceLength": "1000", "PRIME-TransposeXY": "0", "445 AO-TriggerSequenceLength": "1000", "COM1-Name": "COM1", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "COM9-DataBits": "8", "COM1-AnswerTimeout": "500.0000", "Camera": "", "TE2000-Version": "V2.50-V2.50-V2.10", "528 Shutter-Volts": "0.0000", "XLIGHT Hub-Name": "XLIGHT Hub", "PIEZO-Description": "Ludl stage driver adapter", "PositionName": "Pos3", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSampleRate": "100000", "PRIME-FanSpeedSetpoint": "High", "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "405 AO-TriggerSampleRate": "100000", "470 Shutter-Block voltage": "false", "470-State": "0", "COM3-Parity": "None", "405 AO-Volts": "0.0000", "PRIME-TransposeMirrorY": "0", "PRIME-ChipName": "GS144BSI", "470 AO-Volts": "0.0000", "PRIME-TransposeMirrorX": "0", "445 Shutter-SupportsTriggering": "0", "470 Shutter-SupportsTriggering": "0", "XLIGHT Hub-Description": "XLIGHT hub", "PRIME-PreampOffLimit": "10000", "470 AO-Sequenceable": "1", "528 Shutter-MaxVolts": "5.0000", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "Core-XYStage": "XYStage", "470 AO-TriggerSampleRate": "100000", "PRIME-Timing-PreTriggerDelayNs": "24672000.0000", "528-DA Device": "528 Shutter", "Focus-Description": "Nikon TE2000 focus adapter", "FilterBlock-State": "5", "520 AO-Demo": "No", "COM3-DelayBetweenCharsMs": "0.0000", "470 Shutter-Volts": "0.0000", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "PRIME-PP 2 THRESHOLD": "125", "PRIME-PP 4 THRESHOLD": "75", "XYStage-Acceleration": "4.0000", "470 Shutter-Demo": "No", "PRIME-Name": "PMPCIECam00", "640-DA Device": "640 Shutter", "405 AO-Block voltage": "false", "ElapsedTime-ms": 146144, "PRIME-Offset": "100", "445 AO-IOChannel": "Dev1/ao1", "640 AO-MaxVolts": "5.0000", "PositionIndex": 3, "PRIME-PP 1 ENABLED": "Yes", "COM1-BaudRate": "9600", "640-State": "1", "COM3-Verbose": "1", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "LudlController-TransmissionDelay": "0", "PRIME-FullWellCapacity": "62000", "PRIME-SMARTStreamingEnabled": "No", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "640 Shutter-Description": "NI DAC", "528-Description": "DA device that is used as a shutter", "COM4-Description": "Serial port driver (boost:asio)", "405 Shutter-Description": "NI DAC", "TE2000-Name": "TE2000", "528 Shutter-Description": "NI DAC", "COM9-Description": "Serial port driver (boost:asio)", "LudlController-Name": "LudlController", "445 Shutter-TriggerSequenceLength": "1000", "PRIME-SerialNumber": "A18B203008", "445 AO-Volts": "0.0000", "PRIME-X-dimension": "1200", "PRIME-ShutterCloseDelay": "0", "XLIGHT Disk slider-State": "2", "Position": "Default", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 AO-IOChannel": "Dev1/ao5", "520 AO-IOChannel": "Dev1/ao4", "528 AO-TriggerSequenceLength": "1000", "405 Shutter-MaxVolts": "5.0000", "470-DA Device": "470 Shutter", "PRIME-FTCapable": "No", "Core-ChannelGroup": "Channels", "555 AO-SupportsTriggering": "0", "PRIME-Y-dimension": "1200", "Frame": 0, "555 Shutter-Sequenceable": "1", "PRIME-Timing-ExposureTimeNs": "9992000.0000", "Core-ImageProcessor": "", "528-State": "0", "520 AO-Block voltage": "false", "XLIGHT Excitation Wheel-Label": "QUAD", "640 Shutter-Demo": "No", "COM3-StopBits": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "520 Shutter-IOChannel": "Dev1/ao11", "Width": 1200, "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-SupportsTriggering": "0", "640 AO-TriggerSampleRate": "100000", "520-Description": "DA device that is used as a shutter", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "405 Shutter-Sequenceable": "1", "405 Shutter-SupportsTriggering": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-TriggerSampleRate": "100000", "640 Shutter-MaxVolts": "5.0000", "555 AO-MaxVolts": "5.0000", "520 AO-TriggerSequenceLength": "1000", "COM3-BaudRate": "9600", "COM9-BaudRate": "9600", "Vincent-D3-Channel #": "Ch. #1", "PRIME-PMode": "Normal", "OpticalPath-Label": "Left(100)", "BitDepth": 16, "555 AO-TriggerSequenceLength": "1000", "445 Shutter-Description": "NI DAC", "405 AO-Sequenceable": "1", "PRIME-AcquisitionMethod": "Callbacks", "555 AO-Name": "AnalogIO", "PRIME-ShutterMode": "Pre-Exposure", "COM3-Handshaking": "Off", "445 AO-SupportsTriggering": "0", "ROI": "0-0-1200-1200", "COM9-Verbose": "1", "PRIME-Color": "OFF", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "PRIME-ShutterOpenDelay": "0", "COM1-Parity": "None", "Slice": 3, "SlicePosition": -20, "COM9-DelayBetweenCharsMs": "0.0000", "555-Name": "DA Shutter", "PIEZO-LudlSingleAxisName": "B", "XLIGHT Emission Wheel-Label": "679/29 (A)", "640 Shutter-Name": "AnalogIO", "640 AO-Sequenceable": "1", "520 Shutter-Demo": "No", "528 Shutter-Demo": "No", "470-Name": "DA Shutter", "520-DA Device": "520 Shutter", "XYStage-Description": "Ludl XY stage driver adapter", "445 Shutter-MinVolts": "0.0000", "528 AO-TriggerSampleRate": "100000", "405 AO-MinVolts": "0.0000", "405 Shutter-TriggerSequenceLength": "1000", "Height": 1200, "555 AO-Block voltage": "false", "640 Shutter-Volts": "5.0000", "PixelType": "GRAY16", "445 Shutter-Block voltage": "false", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "520 Shutter-TriggerSequenceLength": "1000", "COM9-Name": "COM9", "Core-SLM": "", "555 AO-Volts": "0.0000", "555 Shutter-Block voltage": "false", "XYStage-Speed": "1800.0000", "520 AO-MaxVolts": "5.0000", "528 Shutter-SupportsTriggering": "0", "COM1-Description": "Serial port driver (boost:asio)", "PRIME-MetadataEnabled": "No", "520 Shutter-MinVolts": "0.0000", "470 Shutter-Sequenceable": "1", "PRIME-PreampDelay": "0", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-MinVolts": "0.0000", "ChannelIndex": 2, "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Core-AutoShutter": "1", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "Exposure-ms": 320, "PRIME-PP 2 ENABLED": "Yes", "640 Shutter-TriggerSampleRate": "100000", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "470 Shutter-IOChannel": "Dev1/ao10", "405 AO-Demo": "No", "PixelSizeUm": 1.1, "470 AO-Demo": "No", "470 Shutter-MaxVolts": "5.0000", "Vincent-D3-Name": "Vincent-D3", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FileName": "img_000000000_Il-A_003.tif", "LudlController-Reset": "Operate", "PRIME-Exposure": "320.00", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "445-Name": "DA Shutter", "555 Shutter-Name": "AnalogIO", "520 Shutter-TriggerSampleRate": "100000", "445 Shutter-IOChannel": "Dev1/ao9", "PRIME-TransposeCorrection": "0", "XYStage-StepSize": "0.1000", "445-Description": "DA device that is used as a shutter", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 AO-IOChannel": "Dev1/ao7", "520 Shutter-Name": "AnalogIO", "COM3-DataBits": "8", "640 AO-Volts": "4.5000", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "555 Shutter-IOChannel": "Dev1/ao13", "WaitInterval": null, "520 Shutter-Sequenceable": "1", "YPositionUm": 20690.300000000003, "445-State": "0", "445-DA Device": "445 Shutter", "PRIME-CircularBufferAutoSize": "ON", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "555 AO-TriggerSampleRate": "100000", "445 Shutter-Volts": "0.0000", "XLIGHT Hub-Port": "COM3", "Core-AutoFocus": "", "COM4-Handshaking": "Off", "528 Shutter-MinVolts": "0.0000", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "445 Shutter-Name": "AnalogIO", "528 Shutter-Sequenceable": "1", "XYStage-TransposeMirrorX": "0", "XYStage-TransposeMirrorY": "0", "PRIME-ReadoutRate": "100MHz 16bit", "XYStage-Name": "Stage", "640 AO-SupportsTriggering": "0", "640-Name": "DA Shutter", "640 Shutter-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "640 AO-Block voltage": "false", "445 Shutter-Sequenceable": "1", "470 AO-MaxVolts": "5.0000", "COM4-BaudRate": "9600", "470-Description": "DA device that is used as a shutter", "520 Shutter-Description": "NI DAC", "PRIME-CCDTemperature": "-14.9600", "Time": "2019-12-17 21:58:53 -0500", "640 AO-TriggerSequenceLength": "1000", "470 AO-Name": "AnalogIO", "XLIGHT Disk slider-Label": "Disk Pos 40um", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "Binning": "1x1", "TE2000-ModelType": "1", "COM3-AnswerTimeout": "500.0000", "555 Shutter-SupportsTriggering": "0", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "TE2000-Port": "COM4", "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Description": "NI DAC", "COM4-AnswerTimeout": "500.0000", "COM1-DelayBetweenCharsMs": "0.0000", "FilterBlock-Label": "Confocal", "445 AO-Name": "AnalogIO", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-Timing-ReadoutTimeNs": "24672000.0000", "528 AO-Demo": "No", "520 AO-Sequenceable": "1", "555-Description": "DA device that is used as a shutter", "405-DA Device": "405 Shutter", "405 AO-TriggerSequenceLength": "1000", "640 AO-MinVolts": "0.0000", "640 Shutter-Block voltage": "false", "FrameIndex": 0, "PRIME-Port": "CMOS", "555 AO-MinVolts": "0.0000", "COM3-Description": "Serial port driver (boost:asio)", "528 AO-Description": "NI DAC", "555 AO-IOChannel": "Dev1/ao6", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "555 AO-TriggerInputLine": "/Dev1/PFI0", "PRIME-ActualInterval-ms": "374.3700", "LudlController-Port": "COM9", "445 Shutter-Demo": "No", "PRIME-Trigger-Expose Out-Mux": "1", "SliceIndex": 3, "PRIME-CCDTemperatureSetPoint": "-15.0000", "405 Shutter-MinVolts": "0.0000", "PRIME-ExposeOutMode": "First Row", "AxisPositions": null, "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Volts": "0.0000", "528-Name": "DA Shutter", "555-State": "0", "XLIGHT Dichroic-Label": "Di03-R635-t1", "445 AO-Sequenceable": "1", "405-Name": "DA Shutter", "405 AO-IOChannel": "Dev1/ao0", "Core-Camera": "PRIME", "Focus-Name": "Focus", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "405 Shutter-IOChannel": "Dev1/ao8", "640 AO-Demo": "No", "640 Shutter-SupportsTriggering": "0", "445 AO-Demo": "No", "555-DA Device": "555 Shutter", "470 Shutter-Description": "NI DAC", "528 AO-Volts": "0.0000", "COM1-DataBits": "8", "Vincent-D3-Address": "x", "520 AO-SupportsTriggering": "0", "405-Description": "DA device that is used as a shutter", "640 Shutter-MinVolts": "0.0000", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "405 AO-Name": "AnalogIO", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "640 AO-TriggerInputLine": "/Dev1/PFI0", "405 AO-TriggerInputLine": "/Dev1/PFI0", "640-Description": "DA device that is used as a shutter", "445 Shutter-TriggerSampleRate": "100000", "470 Shutter-TriggerSequenceLength": "1000", "COM1-StopBits": "1", "520 Shutter-SupportsTriggering": "0", "405-State": "0", "Core-Initialize": "1", "XLIGHT Emission Wheel-State": "7", "520 AO-Description": "NI DAC", "528 AO-TriggerInputLine": "/Dev1/PFI0", "445 Shutter-MaxVolts": "5.0000", "COM9-Handshaking": "Off", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "XLIGHT Spin motor-Label": "On (spinning)", "PRIME-PP 5 ENABLED": "No", "UUID": "ebfe6465-c37d-4961-b351-e39446f82651", "PRIME-TriggerMode": "Internal Trigger", "COM1-Handshaking": "Off", "PRIME-Timing-PostTriggerDelayNs": "49344000.0000", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "Focus-Position": "16479.8000", "528 Shutter-Block voltage": "false", "Channel": "Il-A", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "555 Shutter-Demo": "No", "405 Shutter-Demo": "No", "528 AO-Name": "AnalogIO", "520-State": "0", "Core-Galvo": "", "405 Shutter-TriggerSampleRate": "100000", "Focus-FocusStepSize_(nm)": "100", "PRIME-PixelType": "16bit", "COM4-DataBits": "8", "470 AO-IOChannel": "Dev1/ao2", "520 AO-MinVolts": "0.0000", "COM9-Parity": "None", "PRIME-CircularBufferEnabled": "ON", "PRIME-PP 6 Reset": "No", "640 AO-Description": "NI DAC", "Source": "PRIME", "445 AO-MinVolts": "0.0000", "PIEZO-Name": "Stage", "445 AO-Description": "NI DAC", "PRIME-CircularBufferFrameCount": "46", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "520 AO-Volts": "0.0000", "528 Shutter-TriggerSampleRate": "100000", "XPositionUm": 62321, "OpticalPath-State": "4", "PRIME-PP 3 THRESHOLD": "80", "PRIME-PVCAM Version": "3.7.1", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Gain": "1-HDR", "640 Shutter-TriggerSequenceLength": "1000", "528 AO-SupportsTriggering": "0", "445 AO-TriggerInputLine": "/Dev1/PFI0", "OpticalPath-Name": "OpticalPath", "PRIME-PP 4 ENABLED": "Yes", "PRIME-PVCAM Adapter Version": "1.3.29", "COM3-Name": "COM3", "405 AO-MaxVolts": "5.0000", "520 Shutter-Block voltage": "false", "PRIME-Timing-ClearingTimeNs": "49344000.0000", "Core-Focus": "PIEZO", "528 AO-Block voltage": "false", "TE2000-Description": "Nikon TE2000 hub", "528 AO-MinVolts": "0.0000", "COM4-Parity": "None", "Summary": { "Slices": 11, "ChContrastMin": [ 0, 0, 0, 0, 0, 0 ], "PixelAspect": 1, "SlicesFirst": true, "Interval_ms": 0, "Time": "2019-12-17 21:58:39 -0500", "Prefix": "seq_3", "InitialPositionList": [ { "Label": "Pos0", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.435], "Focus": [16480.2], "XYStage": [ 69822.40000000001, 20100.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos1", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.425], "Focus": [16480.3], "XYStage": [ 69004, 20101 ] }, "GridColumnIndex": 0 }, { "Label": "Pos2", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.44], "Focus": [16480.1], "XYStage": [ 63119.5, 20690.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos3", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.405], "Focus": [16480.2], "XYStage": [ 62321, 20690.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos4", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.415], "Focus": [16480], "XYStage": [ 56604.100000000006, 20688.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos5", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.42000000000002], "Focus": [16480.1], "XYStage": [ 56034.200000000004, 20688.800000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos6", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.455], "Focus": [16480], "XYStage": [ 50889.3, 20496.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos7", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.43], "Focus": [16480.2], "XYStage": [ 50101.600000000006, 20496.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos8", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.67000000000002], "Focus": [16479.9], "XYStage": [ 44818.600000000006, 20496.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos9", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.67000000000002], "Focus": [16480], "XYStage": [ 43897.9, 20436.800000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos10", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.675], "Focus": [16479.9], "XYStage": [ 39670.600000000006, 20664.9 ] }, "GridColumnIndex": 0 }, { "Label": "Pos11", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.69], "Focus": [16479.9], "XYStage": [ 38734, 20664.600000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos12", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.695], "Focus": [16480.1], "XYStage": [ 34037.200000000004, 20664.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos13", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.685], "Focus": [16480.1], "XYStage": [ 33152, 20664.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos14", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.69], "Focus": [16480.1], "XYStage": [ 28122.800000000003, 20664.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos15", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.675], "Focus": [16480], "XYStage": [ 27422.9, 20664.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos16", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.64000000000001], "Focus": [16480], "XYStage": [ 23005.2, 20664.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos17", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.69], "Focus": [16479.9], "XYStage": [ 21820.600000000002, 20409.600000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos18", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.41], "Focus": [16480.1], "XYStage": [ 73697.6, -13222.800000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos19", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.455], "Focus": [16480.1], "XYStage": [ 72765.8, -13223 ] }, "GridColumnIndex": 0 }, { "Label": "Pos20", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.4], "Focus": [16480.2], "XYStage": [ 67445.5, -12303.800000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos21", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.415], "Focus": [16480.2], "XYStage": [ 66613.3, -12303.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos22", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.415], "Focus": [16480.1], "XYStage": [ 61819.4, -11936.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos23", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.405], "Focus": [16480.2], "XYStage": [ 61325.700000000004, -11937.1 ] }, "GridColumnIndex": 0 }, { "Label": "Pos24", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.46], "Focus": [16480.2], "XYStage": [ 55377, -10974.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos25", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.445], "Focus": [16480.1], "XYStage": [ 54827, -11214.800000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos26", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.435], "Focus": [16480.2], "XYStage": [ 49937.100000000006, -10919.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos27", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.425], "Focus": [16480.1], "XYStage": [ 48764, -10919.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos28", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.42000000000002], "Focus": [16480.2], "XYStage": [ 43978.4, -10920.400000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos29", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.435], "Focus": [16480.2], "XYStage": [ 43106.3, -10920.400000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos30", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.405], "Focus": [16480.3], "XYStage": [ 38212.4, -10920.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos31", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.445], "Focus": [16480.2], "XYStage": [ 37254.9, -10920.400000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos32", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.44], "Focus": [16480.3], "XYStage": [ 32472.800000000003, -11294.400000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos33", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [166.95000000000002], "Focus": [16480.2], "XYStage": [ 31420.7, -11294.400000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos34", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [166.95000000000002], "Focus": [16480.2], "XYStage": [ 26881.5, -11640 ] }, "GridColumnIndex": 0 }, { "Label": "Pos35", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [166.98], "Focus": [16480.2], "XYStage": [ 25876.100000000002, -11641.1 ] }, "GridColumnIndex": 0 } ], "z-step_um": 10, "GridRow": 0, "BitDepth": 16, "Depth": 2, "Channels": 6, "Source": "Micro-Manager", "ChColors": [ -16711681, -256, -65281, -1, -1, -16776961 ], "MetadataVersion": 10, "KeepShutterOpenChannels": true, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536, 65536 ], "CustomIntervals_ms": [], "Height": 1200, "MicroManagerVersion": "1.4.23 20180219", "GridColumn": 0, "UUID": "8c8f9d20-b128-4577-9f91-89e70f22d319", "PixelType": "GRAY16", "Width": 1200, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\Yan\\20191217-YC113-S5S6", "Frames": 1, "Comment": "", "UserName": "Nikon", "PixelSize_um": 1.1, "ROI": [ 0, 0, 1200, 1200 ], "Date": "2019-12-17", "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC", "P-DAPI-high" ], "KeepShutterOpenSlices": true, "IJType": 1, "ComputerName": "ZadorConfocal2", "Positions": 36, "PositionIndex": 3, "CameraTimeout": 20000, "TimeFirst": false }, "640 AO-Name": "AnalogIO", "Vincent-D3-Command": "Close" }, "FrameKey-0-2-4": { "COM4-StopBits": "1", "528 AO-MaxVolts": "5.0000", "XLIGHT Excitation Wheel-State": "2", "470 Shutter-Name": "AnalogIO", "Core-Shutter": "640", "555 AO-Demo": "No", "XLIGHT Dichroic-State": "2", "XYStage-StepSize-Y": "0.1000", "ZPositionUm": 167.405, "XYStage-StepSize-X": "0.1000", "PRIME-FirmwareVersion": "20.28", "PRIME-ClearCycles": "2", "520-Name": "DA Shutter", "405 Shutter-Name": "AnalogIO", "PIEZO-StepSize": "0.0050", "PRIME-ClearMode": "Pre-Exposure", "470 AO-MinVolts": "0.0000", "405 AO-SupportsTriggering": "1", "CameraChannelIndex": 0, "XYStage-StartSpeed": "500.0000", "COM9-StopBits": "2", "405 AO-Description": "NI DAC", "COM4-DelayBetweenCharsMs": "0.0000", "445 AO-Block voltage": "false", "520 AO-TriggerInputLine": "/Dev1/PFI0", "555 AO-Description": "NI DAC", "520 AO-TriggerSampleRate": "100000", "COM4-Verbose": "1", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM4-Name": "COM4", "405 Shutter-Volts": "0.0000", "Vincent-D3-Port": "COM1", "PRIME-PP 4 MIN ADU AFFECTED": "200", "470 Shutter-MinVolts": "0.0000", "PRIME-PP 3 ENABLED": "Yes", "470 AO-Description": "NI DAC", "PRIME-Description": "PVCAM API device adapter", "520 Shutter-MaxVolts": "5.0000", "520 Shutter-Volts": "0.0000", "528 AO-Sequenceable": "1", "PRIME-Binning": "1x1", "555 Shutter-MaxVolts": "5.0000", "528 Shutter-TriggerSequenceLength": "1000", "405 Shutter-Block voltage": "false", "XLIGHT Spin motor-State": "1", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "Vincent-D3-Time to open (ms)": "35.0000", "Vincent-D3-Time to close (ms)": "35.0000", "445 AO-TriggerSampleRate": "100000", "640 Shutter-IOChannel": "Dev1/ao14", "555 AO-Sequenceable": "1", "520 AO-Name": "AnalogIO", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "470 AO-Block voltage": "false", "COM1-Verbose": "1", "COM9-AnswerTimeout": "10000.0000", "528 Shutter-Name": "AnalogIO", "PRIME-Trigger Timeout (secs)": "10", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "NextFrame": 0, "470 AO-TriggerSequenceLength": "1000", "PRIME-TransposeXY": "0", "445 AO-TriggerSequenceLength": "1000", "COM1-Name": "COM1", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "COM9-DataBits": "8", "COM1-AnswerTimeout": "500.0000", "Camera": "", "TE2000-Version": "V2.50-V2.50-V2.10", "528 Shutter-Volts": "0.0000", "XLIGHT Hub-Name": "XLIGHT Hub", "PIEZO-Description": "Ludl stage driver adapter", "PositionName": "Pos3", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSampleRate": "100000", "PRIME-FanSpeedSetpoint": "High", "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "405 AO-TriggerSampleRate": "100000", "470 Shutter-Block voltage": "false", "470-State": "0", "COM3-Parity": "None", "405 AO-Volts": "0.0000", "PRIME-TransposeMirrorY": "0", "PRIME-ChipName": "GS144BSI", "470 AO-Volts": "0.0000", "PRIME-TransposeMirrorX": "0", "445 Shutter-SupportsTriggering": "0", "470 Shutter-SupportsTriggering": "0", "XLIGHT Hub-Description": "XLIGHT hub", "PRIME-PreampOffLimit": "10000", "470 AO-Sequenceable": "1", "528 Shutter-MaxVolts": "5.0000", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "Core-XYStage": "XYStage", "470 AO-TriggerSampleRate": "100000", "PRIME-Timing-PreTriggerDelayNs": "24672000.0000", "528-DA Device": "528 Shutter", "Focus-Description": "Nikon TE2000 focus adapter", "FilterBlock-State": "5", "520 AO-Demo": "No", "COM3-DelayBetweenCharsMs": "0.0000", "470 Shutter-Volts": "0.0000", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "PRIME-PP 2 THRESHOLD": "125", "PRIME-PP 4 THRESHOLD": "75", "XYStage-Acceleration": "4.0000", "470 Shutter-Demo": "No", "PRIME-Name": "PMPCIECam00", "640-DA Device": "640 Shutter", "405 AO-Block voltage": "false", "ElapsedTime-ms": 146704, "PRIME-Offset": "100", "445 AO-IOChannel": "Dev1/ao1", "640 AO-MaxVolts": "5.0000", "PositionIndex": 3, "PRIME-PP 1 ENABLED": "Yes", "COM1-BaudRate": "9600", "640-State": "1", "COM3-Verbose": "1", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "LudlController-TransmissionDelay": "0", "PRIME-FullWellCapacity": "62000", "PRIME-SMARTStreamingEnabled": "No", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "640 Shutter-Description": "NI DAC", "528-Description": "DA device that is used as a shutter", "COM4-Description": "Serial port driver (boost:asio)", "405 Shutter-Description": "NI DAC", "TE2000-Name": "TE2000", "528 Shutter-Description": "NI DAC", "COM9-Description": "Serial port driver (boost:asio)", "LudlController-Name": "LudlController", "445 Shutter-TriggerSequenceLength": "1000", "PRIME-SerialNumber": "A18B203008", "445 AO-Volts": "0.0000", "PRIME-X-dimension": "1200", "PRIME-ShutterCloseDelay": "0", "XLIGHT Disk slider-State": "2", "Position": "Default", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 AO-IOChannel": "Dev1/ao5", "520 AO-IOChannel": "Dev1/ao4", "528 AO-TriggerSequenceLength": "1000", "405 Shutter-MaxVolts": "5.0000", "470-DA Device": "470 Shutter", "PRIME-FTCapable": "No", "Core-ChannelGroup": "Channels", "555 AO-SupportsTriggering": "0", "PRIME-Y-dimension": "1200", "Frame": 0, "555 Shutter-Sequenceable": "1", "PRIME-Timing-ExposureTimeNs": "9992000.0000", "Core-ImageProcessor": "", "528-State": "0", "520 AO-Block voltage": "false", "XLIGHT Excitation Wheel-Label": "QUAD", "640 Shutter-Demo": "No", "COM3-StopBits": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "520 Shutter-IOChannel": "Dev1/ao11", "Width": 1200, "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-SupportsTriggering": "0", "640 AO-TriggerSampleRate": "100000", "520-Description": "DA device that is used as a shutter", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "405 Shutter-Sequenceable": "1", "405 Shutter-SupportsTriggering": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-TriggerSampleRate": "100000", "640 Shutter-MaxVolts": "5.0000", "555 AO-MaxVolts": "5.0000", "520 AO-TriggerSequenceLength": "1000", "COM3-BaudRate": "9600", "COM9-BaudRate": "9600", "Vincent-D3-Channel #": "Ch. #1", "PRIME-PMode": "Normal", "OpticalPath-Label": "Left(100)", "BitDepth": 16, "555 AO-TriggerSequenceLength": "1000", "445 Shutter-Description": "NI DAC", "405 AO-Sequenceable": "1", "PRIME-AcquisitionMethod": "Callbacks", "555 AO-Name": "AnalogIO", "PRIME-ShutterMode": "Pre-Exposure", "COM3-Handshaking": "Off", "445 AO-SupportsTriggering": "0", "ROI": "0-0-1200-1200", "COM9-Verbose": "1", "PRIME-Color": "OFF", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "PRIME-ShutterOpenDelay": "0", "COM1-Parity": "None", "Slice": 4, "SlicePosition": -10, "COM9-DelayBetweenCharsMs": "0.0000", "555-Name": "DA Shutter", "PIEZO-LudlSingleAxisName": "B", "XLIGHT Emission Wheel-Label": "679/29 (A)", "640 Shutter-Name": "AnalogIO", "640 AO-Sequenceable": "1", "520 Shutter-Demo": "No", "528 Shutter-Demo": "No", "470-Name": "DA Shutter", "520-DA Device": "520 Shutter", "XYStage-Description": "Ludl XY stage driver adapter", "445 Shutter-MinVolts": "0.0000", "528 AO-TriggerSampleRate": "100000", "405 AO-MinVolts": "0.0000", "405 Shutter-TriggerSequenceLength": "1000", "Height": 1200, "555 AO-Block voltage": "false", "640 Shutter-Volts": "5.0000", "PixelType": "GRAY16", "445 Shutter-Block voltage": "false", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "520 Shutter-TriggerSequenceLength": "1000", "COM9-Name": "COM9", "Core-SLM": "", "555 AO-Volts": "0.0000", "555 Shutter-Block voltage": "false", "XYStage-Speed": "1800.0000", "520 AO-MaxVolts": "5.0000", "528 Shutter-SupportsTriggering": "0", "COM1-Description": "Serial port driver (boost:asio)", "PRIME-MetadataEnabled": "No", "520 Shutter-MinVolts": "0.0000", "470 Shutter-Sequenceable": "1", "PRIME-PreampDelay": "0", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-MinVolts": "0.0000", "ChannelIndex": 2, "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Core-AutoShutter": "1", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "Exposure-ms": 320, "PRIME-PP 2 ENABLED": "Yes", "640 Shutter-TriggerSampleRate": "100000", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "470 Shutter-IOChannel": "Dev1/ao10", "405 AO-Demo": "No", "PixelSizeUm": 1.1, "470 AO-Demo": "No", "470 Shutter-MaxVolts": "5.0000", "Vincent-D3-Name": "Vincent-D3", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FileName": "img_000000000_Il-A_004.tif", "LudlController-Reset": "Operate", "PRIME-Exposure": "320.00", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "445-Name": "DA Shutter", "555 Shutter-Name": "AnalogIO", "520 Shutter-TriggerSampleRate": "100000", "445 Shutter-IOChannel": "Dev1/ao9", "PRIME-TransposeCorrection": "0", "XYStage-StepSize": "0.1000", "445-Description": "DA device that is used as a shutter", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 AO-IOChannel": "Dev1/ao7", "520 Shutter-Name": "AnalogIO", "COM3-DataBits": "8", "640 AO-Volts": "4.5000", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "555 Shutter-IOChannel": "Dev1/ao13", "WaitInterval": null, "520 Shutter-Sequenceable": "1", "YPositionUm": 20690.300000000003, "445-State": "0", "445-DA Device": "445 Shutter", "PRIME-CircularBufferAutoSize": "ON", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "555 AO-TriggerSampleRate": "100000", "445 Shutter-Volts": "0.0000", "XLIGHT Hub-Port": "COM3", "Core-AutoFocus": "", "COM4-Handshaking": "Off", "528 Shutter-MinVolts": "0.0000", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "445 Shutter-Name": "AnalogIO", "528 Shutter-Sequenceable": "1", "XYStage-TransposeMirrorX": "0", "XYStage-TransposeMirrorY": "0", "PRIME-ReadoutRate": "100MHz 16bit", "XYStage-Name": "Stage", "640 AO-SupportsTriggering": "0", "640-Name": "DA Shutter", "640 Shutter-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "640 AO-Block voltage": "false", "445 Shutter-Sequenceable": "1", "470 AO-MaxVolts": "5.0000", "COM4-BaudRate": "9600", "470-Description": "DA device that is used as a shutter", "520 Shutter-Description": "NI DAC", "PRIME-CCDTemperature": "-14.9600", "Time": "2019-12-17 21:58:54 -0500", "640 AO-TriggerSequenceLength": "1000", "470 AO-Name": "AnalogIO", "XLIGHT Disk slider-Label": "Disk Pos 40um", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "Binning": "1x1", "TE2000-ModelType": "1", "COM3-AnswerTimeout": "500.0000", "555 Shutter-SupportsTriggering": "0", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "TE2000-Port": "COM4", "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Description": "NI DAC", "COM4-AnswerTimeout": "500.0000", "COM1-DelayBetweenCharsMs": "0.0000", "FilterBlock-Label": "Confocal", "445 AO-Name": "AnalogIO", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-Timing-ReadoutTimeNs": "24672000.0000", "528 AO-Demo": "No", "520 AO-Sequenceable": "1", "555-Description": "DA device that is used as a shutter", "405-DA Device": "405 Shutter", "405 AO-TriggerSequenceLength": "1000", "640 AO-MinVolts": "0.0000", "640 Shutter-Block voltage": "false", "FrameIndex": 0, "PRIME-Port": "CMOS", "555 AO-MinVolts": "0.0000", "COM3-Description": "Serial port driver (boost:asio)", "528 AO-Description": "NI DAC", "555 AO-IOChannel": "Dev1/ao6", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "555 AO-TriggerInputLine": "/Dev1/PFI0", "PRIME-ActualInterval-ms": "374.3700", "LudlController-Port": "COM9", "445 Shutter-Demo": "No", "PRIME-Trigger-Expose Out-Mux": "1", "SliceIndex": 4, "PRIME-CCDTemperatureSetPoint": "-15.0000", "405 Shutter-MinVolts": "0.0000", "PRIME-ExposeOutMode": "First Row", "AxisPositions": null, "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Volts": "0.0000", "528-Name": "DA Shutter", "555-State": "0", "XLIGHT Dichroic-Label": "Di03-R635-t1", "445 AO-Sequenceable": "1", "405-Name": "DA Shutter", "405 AO-IOChannel": "Dev1/ao0", "Core-Camera": "PRIME", "Focus-Name": "Focus", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "405 Shutter-IOChannel": "Dev1/ao8", "640 AO-Demo": "No", "640 Shutter-SupportsTriggering": "0", "445 AO-Demo": "No", "555-DA Device": "555 Shutter", "470 Shutter-Description": "NI DAC", "528 AO-Volts": "0.0000", "COM1-DataBits": "8", "Vincent-D3-Address": "x", "520 AO-SupportsTriggering": "0", "405-Description": "DA device that is used as a shutter", "640 Shutter-MinVolts": "0.0000", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "405 AO-Name": "AnalogIO", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "640 AO-TriggerInputLine": "/Dev1/PFI0", "405 AO-TriggerInputLine": "/Dev1/PFI0", "640-Description": "DA device that is used as a shutter", "445 Shutter-TriggerSampleRate": "100000", "470 Shutter-TriggerSequenceLength": "1000", "COM1-StopBits": "1", "520 Shutter-SupportsTriggering": "0", "405-State": "0", "Core-Initialize": "1", "XLIGHT Emission Wheel-State": "7", "520 AO-Description": "NI DAC", "528 AO-TriggerInputLine": "/Dev1/PFI0", "445 Shutter-MaxVolts": "5.0000", "COM9-Handshaking": "Off", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "XLIGHT Spin motor-Label": "On (spinning)", "PRIME-PP 5 ENABLED": "No", "UUID": "8a6c5a84-9815-45e8-a8aa-ef907d9a5498", "PRIME-TriggerMode": "Internal Trigger", "COM1-Handshaking": "Off", "PRIME-Timing-PostTriggerDelayNs": "49344000.0000", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "Focus-Position": "16479.8000", "528 Shutter-Block voltage": "false", "Channel": "Il-A", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "555 Shutter-Demo": "No", "405 Shutter-Demo": "No", "528 AO-Name": "AnalogIO", "520-State": "0", "Core-Galvo": "", "405 Shutter-TriggerSampleRate": "100000", "Focus-FocusStepSize_(nm)": "100", "PRIME-PixelType": "16bit", "COM4-DataBits": "8", "470 AO-IOChannel": "Dev1/ao2", "520 AO-MinVolts": "0.0000", "COM9-Parity": "None", "PRIME-CircularBufferEnabled": "ON", "PRIME-PP 6 Reset": "No", "640 AO-Description": "NI DAC", "Source": "PRIME", "445 AO-MinVolts": "0.0000", "PIEZO-Name": "Stage", "445 AO-Description": "NI DAC", "PRIME-CircularBufferFrameCount": "46", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "520 AO-Volts": "0.0000", "528 Shutter-TriggerSampleRate": "100000", "XPositionUm": 62321, "OpticalPath-State": "4", "PRIME-PP 3 THRESHOLD": "80", "PRIME-PVCAM Version": "3.7.1", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Gain": "1-HDR", "640 Shutter-TriggerSequenceLength": "1000", "528 AO-SupportsTriggering": "0", "445 AO-TriggerInputLine": "/Dev1/PFI0", "OpticalPath-Name": "OpticalPath", "PRIME-PP 4 ENABLED": "Yes", "PRIME-PVCAM Adapter Version": "1.3.29", "COM3-Name": "COM3", "405 AO-MaxVolts": "5.0000", "520 Shutter-Block voltage": "false", "PRIME-Timing-ClearingTimeNs": "49344000.0000", "Core-Focus": "PIEZO", "528 AO-Block voltage": "false", "TE2000-Description": "Nikon TE2000 hub", "528 AO-MinVolts": "0.0000", "COM4-Parity": "None", "Summary": { "Slices": 11, "ChContrastMin": [ 0, 0, 0, 0, 0, 0 ], "PixelAspect": 1, "SlicesFirst": true, "Interval_ms": 0, "Time": "2019-12-17 21:58:39 -0500", "Prefix": "seq_3", "InitialPositionList": [ { "Label": "Pos0", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.435], "Focus": [16480.2], "XYStage": [ 69822.40000000001, 20100.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos1", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.425], "Focus": [16480.3], "XYStage": [ 69004, 20101 ] }, "GridColumnIndex": 0 }, { "Label": "Pos2", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.44], "Focus": [16480.1], "XYStage": [ 63119.5, 20690.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos3", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.405], "Focus": [16480.2], "XYStage": [ 62321, 20690.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos4", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.415], "Focus": [16480], "XYStage": [ 56604.100000000006, 20688.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos5", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.42000000000002], "Focus": [16480.1], "XYStage": [ 56034.200000000004, 20688.800000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos6", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.455], "Focus": [16480], "XYStage": [ 50889.3, 20496.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos7", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.43], "Focus": [16480.2], "XYStage": [ 50101.600000000006, 20496.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos8", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.67000000000002], "Focus": [16479.9], "XYStage": [ 44818.600000000006, 20496.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos9", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.67000000000002], "Focus": [16480], "XYStage": [ 43897.9, 20436.800000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos10", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.675], "Focus": [16479.9], "XYStage": [ 39670.600000000006, 20664.9 ] }, "GridColumnIndex": 0 }, { "Label": "Pos11", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.69], "Focus": [16479.9], "XYStage": [ 38734, 20664.600000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos12", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.695], "Focus": [16480.1], "XYStage": [ 34037.200000000004, 20664.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos13", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.685], "Focus": [16480.1], "XYStage": [ 33152, 20664.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos14", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.69], "Focus": [16480.1], "XYStage": [ 28122.800000000003, 20664.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos15", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.675], "Focus": [16480], "XYStage": [ 27422.9, 20664.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos16", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.64000000000001], "Focus": [16480], "XYStage": [ 23005.2, 20664.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos17", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.69], "Focus": [16479.9], "XYStage": [ 21820.600000000002, 20409.600000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos18", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.41], "Focus": [16480.1], "XYStage": [ 73697.6, -13222.800000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos19", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.455], "Focus": [16480.1], "XYStage": [ 72765.8, -13223 ] }, "GridColumnIndex": 0 }, { "Label": "Pos20", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.4], "Focus": [16480.2], "XYStage": [ 67445.5, -12303.800000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos21", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.415], "Focus": [16480.2], "XYStage": [ 66613.3, -12303.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos22", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.415], "Focus": [16480.1], "XYStage": [ 61819.4, -11936.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos23", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.405], "Focus": [16480.2], "XYStage": [ 61325.700000000004, -11937.1 ] }, "GridColumnIndex": 0 }, { "Label": "Pos24", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.46], "Focus": [16480.2], "XYStage": [ 55377, -10974.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos25", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.445], "Focus": [16480.1], "XYStage": [ 54827, -11214.800000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos26", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.435], "Focus": [16480.2], "XYStage": [ 49937.100000000006, -10919.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos27", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.425], "Focus": [16480.1], "XYStage": [ 48764, -10919.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos28", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.42000000000002], "Focus": [16480.2], "XYStage": [ 43978.4, -10920.400000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos29", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.435], "Focus": [16480.2], "XYStage": [ 43106.3, -10920.400000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos30", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.405], "Focus": [16480.3], "XYStage": [ 38212.4, -10920.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos31", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.445], "Focus": [16480.2], "XYStage": [ 37254.9, -10920.400000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos32", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.44], "Focus": [16480.3], "XYStage": [ 32472.800000000003, -11294.400000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos33", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [166.95000000000002], "Focus": [16480.2], "XYStage": [ 31420.7, -11294.400000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos34", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [166.95000000000002], "Focus": [16480.2], "XYStage": [ 26881.5, -11640 ] }, "GridColumnIndex": 0 }, { "Label": "Pos35", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [166.98], "Focus": [16480.2], "XYStage": [ 25876.100000000002, -11641.1 ] }, "GridColumnIndex": 0 } ], "z-step_um": 10, "GridRow": 0, "BitDepth": 16, "Depth": 2, "Channels": 6, "Source": "Micro-Manager", "ChColors": [ -16711681, -256, -65281, -1, -1, -16776961 ], "MetadataVersion": 10, "KeepShutterOpenChannels": true, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536, 65536 ], "CustomIntervals_ms": [], "Height": 1200, "MicroManagerVersion": "1.4.23 20180219", "GridColumn": 0, "UUID": "8c8f9d20-b128-4577-9f91-89e70f22d319", "PixelType": "GRAY16", "Width": 1200, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\Yan\\20191217-YC113-S5S6", "Frames": 1, "Comment": "", "UserName": "Nikon", "PixelSize_um": 1.1, "ROI": [ 0, 0, 1200, 1200 ], "Date": "2019-12-17", "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC", "P-DAPI-high" ], "KeepShutterOpenSlices": true, "IJType": 1, "ComputerName": "ZadorConfocal2", "Positions": 36, "PositionIndex": 3, "CameraTimeout": 20000, "TimeFirst": false }, "640 AO-Name": "AnalogIO", "Vincent-D3-Command": "Close" }, "FrameKey-0-2-5": { "COM4-StopBits": "1", "528 AO-MaxVolts": "5.0000", "XLIGHT Excitation Wheel-State": "2", "470 Shutter-Name": "AnalogIO", "Core-Shutter": "640", "555 AO-Demo": "No", "XLIGHT Dichroic-State": "2", "XYStage-StepSize-Y": "0.1000", "ZPositionUm": 177.405, "XYStage-StepSize-X": "0.1000", "PRIME-FirmwareVersion": "20.28", "PRIME-ClearCycles": "2", "520-Name": "DA Shutter", "405 Shutter-Name": "AnalogIO", "PIEZO-StepSize": "0.0050", "PRIME-ClearMode": "Pre-Exposure", "470 AO-MinVolts": "0.0000", "405 AO-SupportsTriggering": "1", "CameraChannelIndex": 0, "XYStage-StartSpeed": "500.0000", "COM9-StopBits": "2", "405 AO-Description": "NI DAC", "COM4-DelayBetweenCharsMs": "0.0000", "445 AO-Block voltage": "false", "520 AO-TriggerInputLine": "/Dev1/PFI0", "555 AO-Description": "NI DAC", "520 AO-TriggerSampleRate": "100000", "COM4-Verbose": "1", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM4-Name": "COM4", "405 Shutter-Volts": "0.0000", "Vincent-D3-Port": "COM1", "PRIME-PP 4 MIN ADU AFFECTED": "200", "470 Shutter-MinVolts": "0.0000", "PRIME-PP 3 ENABLED": "Yes", "470 AO-Description": "NI DAC", "PRIME-Description": "PVCAM API device adapter", "520 Shutter-MaxVolts": "5.0000", "520 Shutter-Volts": "0.0000", "528 AO-Sequenceable": "1", "PRIME-Binning": "1x1", "555 Shutter-MaxVolts": "5.0000", "528 Shutter-TriggerSequenceLength": "1000", "405 Shutter-Block voltage": "false", "XLIGHT Spin motor-State": "1", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "Vincent-D3-Time to open (ms)": "35.0000", "Vincent-D3-Time to close (ms)": "35.0000", "445 AO-TriggerSampleRate": "100000", "640 Shutter-IOChannel": "Dev1/ao14", "555 AO-Sequenceable": "1", "520 AO-Name": "AnalogIO", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "470 AO-Block voltage": "false", "COM1-Verbose": "1", "COM9-AnswerTimeout": "10000.0000", "528 Shutter-Name": "AnalogIO", "PRIME-Trigger Timeout (secs)": "10", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "NextFrame": 0, "470 AO-TriggerSequenceLength": "1000", "PRIME-TransposeXY": "0", "445 AO-TriggerSequenceLength": "1000", "COM1-Name": "COM1", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "COM9-DataBits": "8", "COM1-AnswerTimeout": "500.0000", "Camera": "", "TE2000-Version": "V2.50-V2.50-V2.10", "528 Shutter-Volts": "0.0000", "XLIGHT Hub-Name": "XLIGHT Hub", "PIEZO-Description": "Ludl stage driver adapter", "PositionName": "Pos3", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSampleRate": "100000", "PRIME-FanSpeedSetpoint": "High", "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "405 AO-TriggerSampleRate": "100000", "470 Shutter-Block voltage": "false", "470-State": "0", "COM3-Parity": "None", "405 AO-Volts": "0.0000", "PRIME-TransposeMirrorY": "0", "PRIME-ChipName": "GS144BSI", "470 AO-Volts": "0.0000", "PRIME-TransposeMirrorX": "0", "445 Shutter-SupportsTriggering": "0", "470 Shutter-SupportsTriggering": "0", "XLIGHT Hub-Description": "XLIGHT hub", "PRIME-PreampOffLimit": "10000", "470 AO-Sequenceable": "1", "528 Shutter-MaxVolts": "5.0000", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "Core-XYStage": "XYStage", "470 AO-TriggerSampleRate": "100000", "PRIME-Timing-PreTriggerDelayNs": "24672000.0000", "528-DA Device": "528 Shutter", "Focus-Description": "Nikon TE2000 focus adapter", "FilterBlock-State": "5", "520 AO-Demo": "No", "COM3-DelayBetweenCharsMs": "0.0000", "470 Shutter-Volts": "0.0000", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "PRIME-PP 2 THRESHOLD": "125", "PRIME-PP 4 THRESHOLD": "75", "XYStage-Acceleration": "4.0000", "470 Shutter-Demo": "No", "PRIME-Name": "PMPCIECam00", "640-DA Device": "640 Shutter", "405 AO-Block voltage": "false", "ElapsedTime-ms": 147264, "PRIME-Offset": "100", "445 AO-IOChannel": "Dev1/ao1", "640 AO-MaxVolts": "5.0000", "PositionIndex": 3, "PRIME-PP 1 ENABLED": "Yes", "COM1-BaudRate": "9600", "640-State": "1", "COM3-Verbose": "1", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "LudlController-TransmissionDelay": "0", "PRIME-FullWellCapacity": "62000", "PRIME-SMARTStreamingEnabled": "No", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "640 Shutter-Description": "NI DAC", "528-Description": "DA device that is used as a shutter", "COM4-Description": "Serial port driver (boost:asio)", "405 Shutter-Description": "NI DAC", "TE2000-Name": "TE2000", "528 Shutter-Description": "NI DAC", "COM9-Description": "Serial port driver (boost:asio)", "LudlController-Name": "LudlController", "445 Shutter-TriggerSequenceLength": "1000", "PRIME-SerialNumber": "A18B203008", "445 AO-Volts": "0.0000", "PRIME-X-dimension": "1200", "PRIME-ShutterCloseDelay": "0", "XLIGHT Disk slider-State": "2", "Position": "Default", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 AO-IOChannel": "Dev1/ao5", "520 AO-IOChannel": "Dev1/ao4", "528 AO-TriggerSequenceLength": "1000", "405 Shutter-MaxVolts": "5.0000", "470-DA Device": "470 Shutter", "PRIME-FTCapable": "No", "Core-ChannelGroup": "Channels", "555 AO-SupportsTriggering": "0", "PRIME-Y-dimension": "1200", "Frame": 0, "555 Shutter-Sequenceable": "1", "PRIME-Timing-ExposureTimeNs": "9992000.0000", "Core-ImageProcessor": "", "528-State": "0", "520 AO-Block voltage": "false", "XLIGHT Excitation Wheel-Label": "QUAD", "640 Shutter-Demo": "No", "COM3-StopBits": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "520 Shutter-IOChannel": "Dev1/ao11", "Width": 1200, "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-SupportsTriggering": "0", "640 AO-TriggerSampleRate": "100000", "520-Description": "DA device that is used as a shutter", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "405 Shutter-Sequenceable": "1", "405 Shutter-SupportsTriggering": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-TriggerSampleRate": "100000", "640 Shutter-MaxVolts": "5.0000", "555 AO-MaxVolts": "5.0000", "520 AO-TriggerSequenceLength": "1000", "COM3-BaudRate": "9600", "COM9-BaudRate": "9600", "Vincent-D3-Channel #": "Ch. #1", "PRIME-PMode": "Normal", "OpticalPath-Label": "Left(100)", "BitDepth": 16, "555 AO-TriggerSequenceLength": "1000", "445 Shutter-Description": "NI DAC", "405 AO-Sequenceable": "1", "PRIME-AcquisitionMethod": "Callbacks", "555 AO-Name": "AnalogIO", "PRIME-ShutterMode": "Pre-Exposure", "COM3-Handshaking": "Off", "445 AO-SupportsTriggering": "0", "ROI": "0-0-1200-1200", "COM9-Verbose": "1", "PRIME-Color": "OFF", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "PRIME-ShutterOpenDelay": "0", "COM1-Parity": "None", "Slice": 5, "SlicePosition": 0, "COM9-DelayBetweenCharsMs": "0.0000", "555-Name": "DA Shutter", "PIEZO-LudlSingleAxisName": "B", "XLIGHT Emission Wheel-Label": "679/29 (A)", "640 Shutter-Name": "AnalogIO", "640 AO-Sequenceable": "1", "520 Shutter-Demo": "No", "528 Shutter-Demo": "No", "470-Name": "DA Shutter", "520-DA Device": "520 Shutter", "XYStage-Description": "Ludl XY stage driver adapter", "445 Shutter-MinVolts": "0.0000", "528 AO-TriggerSampleRate": "100000", "405 AO-MinVolts": "0.0000", "405 Shutter-TriggerSequenceLength": "1000", "Height": 1200, "555 AO-Block voltage": "false", "640 Shutter-Volts": "5.0000", "PixelType": "GRAY16", "445 Shutter-Block voltage": "false", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "520 Shutter-TriggerSequenceLength": "1000", "COM9-Name": "COM9", "Core-SLM": "", "555 AO-Volts": "0.0000", "555 Shutter-Block voltage": "false", "XYStage-Speed": "1800.0000", "520 AO-MaxVolts": "5.0000", "528 Shutter-SupportsTriggering": "0", "COM1-Description": "Serial port driver (boost:asio)", "PRIME-MetadataEnabled": "No", "520 Shutter-MinVolts": "0.0000", "470 Shutter-Sequenceable": "1", "PRIME-PreampDelay": "0", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-MinVolts": "0.0000", "ChannelIndex": 2, "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Core-AutoShutter": "1", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "Exposure-ms": 320, "PRIME-PP 2 ENABLED": "Yes", "640 Shutter-TriggerSampleRate": "100000", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "470 Shutter-IOChannel": "Dev1/ao10", "405 AO-Demo": "No", "PixelSizeUm": 1.1, "470 AO-Demo": "No", "470 Shutter-MaxVolts": "5.0000", "Vincent-D3-Name": "Vincent-D3", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FileName": "img_000000000_Il-A_005.tif", "LudlController-Reset": "Operate", "PRIME-Exposure": "320.00", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "445-Name": "DA Shutter", "555 Shutter-Name": "AnalogIO", "520 Shutter-TriggerSampleRate": "100000", "445 Shutter-IOChannel": "Dev1/ao9", "PRIME-TransposeCorrection": "0", "XYStage-StepSize": "0.1000", "445-Description": "DA device that is used as a shutter", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 AO-IOChannel": "Dev1/ao7", "520 Shutter-Name": "AnalogIO", "COM3-DataBits": "8", "640 AO-Volts": "4.5000", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "555 Shutter-IOChannel": "Dev1/ao13", "WaitInterval": null, "520 Shutter-Sequenceable": "1", "YPositionUm": 20690.300000000003, "445-State": "0", "445-DA Device": "445 Shutter", "PRIME-CircularBufferAutoSize": "ON", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "555 AO-TriggerSampleRate": "100000", "445 Shutter-Volts": "0.0000", "XLIGHT Hub-Port": "COM3", "Core-AutoFocus": "", "COM4-Handshaking": "Off", "528 Shutter-MinVolts": "0.0000", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "445 Shutter-Name": "AnalogIO", "528 Shutter-Sequenceable": "1", "XYStage-TransposeMirrorX": "0", "XYStage-TransposeMirrorY": "0", "PRIME-ReadoutRate": "100MHz 16bit", "XYStage-Name": "Stage", "640 AO-SupportsTriggering": "0", "640-Name": "DA Shutter", "640 Shutter-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "640 AO-Block voltage": "false", "445 Shutter-Sequenceable": "1", "470 AO-MaxVolts": "5.0000", "COM4-BaudRate": "9600", "470-Description": "DA device that is used as a shutter", "520 Shutter-Description": "NI DAC", "PRIME-CCDTemperature": "-14.9600", "Time": "2019-12-17 21:58:54 -0500", "640 AO-TriggerSequenceLength": "1000", "470 AO-Name": "AnalogIO", "XLIGHT Disk slider-Label": "Disk Pos 40um", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "Binning": "1x1", "TE2000-ModelType": "1", "COM3-AnswerTimeout": "500.0000", "555 Shutter-SupportsTriggering": "0", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "TE2000-Port": "COM4", "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Description": "NI DAC", "COM4-AnswerTimeout": "500.0000", "COM1-DelayBetweenCharsMs": "0.0000", "FilterBlock-Label": "Confocal", "445 AO-Name": "AnalogIO", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-Timing-ReadoutTimeNs": "24672000.0000", "528 AO-Demo": "No", "520 AO-Sequenceable": "1", "555-Description": "DA device that is used as a shutter", "405-DA Device": "405 Shutter", "405 AO-TriggerSequenceLength": "1000", "640 AO-MinVolts": "0.0000", "640 Shutter-Block voltage": "false", "FrameIndex": 0, "PRIME-Port": "CMOS", "555 AO-MinVolts": "0.0000", "COM3-Description": "Serial port driver (boost:asio)", "528 AO-Description": "NI DAC", "555 AO-IOChannel": "Dev1/ao6", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "555 AO-TriggerInputLine": "/Dev1/PFI0", "PRIME-ActualInterval-ms": "374.3700", "LudlController-Port": "COM9", "445 Shutter-Demo": "No", "PRIME-Trigger-Expose Out-Mux": "1", "SliceIndex": 5, "PRIME-CCDTemperatureSetPoint": "-15.0000", "405 Shutter-MinVolts": "0.0000", "PRIME-ExposeOutMode": "First Row", "AxisPositions": null, "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Volts": "0.0000", "528-Name": "DA Shutter", "555-State": "0", "XLIGHT Dichroic-Label": "Di03-R635-t1", "445 AO-Sequenceable": "1", "405-Name": "DA Shutter", "405 AO-IOChannel": "Dev1/ao0", "Core-Camera": "PRIME", "Focus-Name": "Focus", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "405 Shutter-IOChannel": "Dev1/ao8", "640 AO-Demo": "No", "640 Shutter-SupportsTriggering": "0", "445 AO-Demo": "No", "555-DA Device": "555 Shutter", "470 Shutter-Description": "NI DAC", "528 AO-Volts": "0.0000", "COM1-DataBits": "8", "Vincent-D3-Address": "x", "520 AO-SupportsTriggering": "0", "405-Description": "DA device that is used as a shutter", "640 Shutter-MinVolts": "0.0000", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "405 AO-Name": "AnalogIO", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "640 AO-TriggerInputLine": "/Dev1/PFI0", "405 AO-TriggerInputLine": "/Dev1/PFI0", "640-Description": "DA device that is used as a shutter", "445 Shutter-TriggerSampleRate": "100000", "470 Shutter-TriggerSequenceLength": "1000", "COM1-StopBits": "1", "520 Shutter-SupportsTriggering": "0", "405-State": "0", "Core-Initialize": "1", "XLIGHT Emission Wheel-State": "7", "520 AO-Description": "NI DAC", "528 AO-TriggerInputLine": "/Dev1/PFI0", "445 Shutter-MaxVolts": "5.0000", "COM9-Handshaking": "Off", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "XLIGHT Spin motor-Label": "On (spinning)", "PRIME-PP 5 ENABLED": "No", "UUID": "99249136-f34f-4f6f-81be-3967911a234e", "PRIME-TriggerMode": "Internal Trigger", "COM1-Handshaking": "Off", "PRIME-Timing-PostTriggerDelayNs": "49344000.0000", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "Focus-Position": "16479.8000", "528 Shutter-Block voltage": "false", "Channel": "Il-A", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "555 Shutter-Demo": "No", "405 Shutter-Demo": "No", "528 AO-Name": "AnalogIO", "520-State": "0", "Core-Galvo": "", "405 Shutter-TriggerSampleRate": "100000", "Focus-FocusStepSize_(nm)": "100", "PRIME-PixelType": "16bit", "COM4-DataBits": "8", "470 AO-IOChannel": "Dev1/ao2", "520 AO-MinVolts": "0.0000", "COM9-Parity": "None", "PRIME-CircularBufferEnabled": "ON", "PRIME-PP 6 Reset": "No", "640 AO-Description": "NI DAC", "Source": "PRIME", "445 AO-MinVolts": "0.0000", "PIEZO-Name": "Stage", "445 AO-Description": "NI DAC", "PRIME-CircularBufferFrameCount": "46", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "520 AO-Volts": "0.0000", "528 Shutter-TriggerSampleRate": "100000", "XPositionUm": 62321, "OpticalPath-State": "4", "PRIME-PP 3 THRESHOLD": "80", "PRIME-PVCAM Version": "3.7.1", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Gain": "1-HDR", "640 Shutter-TriggerSequenceLength": "1000", "528 AO-SupportsTriggering": "0", "445 AO-TriggerInputLine": "/Dev1/PFI0", "OpticalPath-Name": "OpticalPath", "PRIME-PP 4 ENABLED": "Yes", "PRIME-PVCAM Adapter Version": "1.3.29", "COM3-Name": "COM3", "405 AO-MaxVolts": "5.0000", "520 Shutter-Block voltage": "false", "PRIME-Timing-ClearingTimeNs": "49344000.0000", "Core-Focus": "PIEZO", "528 AO-Block voltage": "false", "TE2000-Description": "Nikon TE2000 hub", "528 AO-MinVolts": "0.0000", "COM4-Parity": "None", "Summary": { "Slices": 11, "ChContrastMin": [ 0, 0, 0, 0, 0, 0 ], "PixelAspect": 1, "SlicesFirst": true, "Interval_ms": 0, "Time": "2019-12-17 21:58:39 -0500", "Prefix": "seq_3", "InitialPositionList": [ { "Label": "Pos0", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.435], "Focus": [16480.2], "XYStage": [ 69822.40000000001, 20100.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos1", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.425], "Focus": [16480.3], "XYStage": [ 69004, 20101 ] }, "GridColumnIndex": 0 }, { "Label": "Pos2", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.44], "Focus": [16480.1], "XYStage": [ 63119.5, 20690.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos3", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.405], "Focus": [16480.2], "XYStage": [ 62321, 20690.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos4", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.415], "Focus": [16480], "XYStage": [ 56604.100000000006, 20688.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos5", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.42000000000002], "Focus": [16480.1], "XYStage": [ 56034.200000000004, 20688.800000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos6", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.455], "Focus": [16480], "XYStage": [ 50889.3, 20496.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos7", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.43], "Focus": [16480.2], "XYStage": [ 50101.600000000006, 20496.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos8", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.67000000000002], "Focus": [16479.9], "XYStage": [ 44818.600000000006, 20496.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos9", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.67000000000002], "Focus": [16480], "XYStage": [ 43897.9, 20436.800000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos10", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.675], "Focus": [16479.9], "XYStage": [ 39670.600000000006, 20664.9 ] }, "GridColumnIndex": 0 }, { "Label": "Pos11", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.69], "Focus": [16479.9], "XYStage": [ 38734, 20664.600000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos12", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.695], "Focus": [16480.1], "XYStage": [ 34037.200000000004, 20664.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos13", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.685], "Focus": [16480.1], "XYStage": [ 33152, 20664.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos14", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.69], "Focus": [16480.1], "XYStage": [ 28122.800000000003, 20664.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos15", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.675], "Focus": [16480], "XYStage": [ 27422.9, 20664.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos16", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.64000000000001], "Focus": [16480], "XYStage": [ 23005.2, 20664.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos17", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.69], "Focus": [16479.9], "XYStage": [ 21820.600000000002, 20409.600000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos18", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.41], "Focus": [16480.1], "XYStage": [ 73697.6, -13222.800000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos19", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.455], "Focus": [16480.1], "XYStage": [ 72765.8, -13223 ] }, "GridColumnIndex": 0 }, { "Label": "Pos20", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.4], "Focus": [16480.2], "XYStage": [ 67445.5, -12303.800000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos21", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.415], "Focus": [16480.2], "XYStage": [ 66613.3, -12303.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos22", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.415], "Focus": [16480.1], "XYStage": [ 61819.4, -11936.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos23", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.405], "Focus": [16480.2], "XYStage": [ 61325.700000000004, -11937.1 ] }, "GridColumnIndex": 0 }, { "Label": "Pos24", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.46], "Focus": [16480.2], "XYStage": [ 55377, -10974.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos25", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.445], "Focus": [16480.1], "XYStage": [ 54827, -11214.800000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos26", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.435], "Focus": [16480.2], "XYStage": [ 49937.100000000006, -10919.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos27", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.425], "Focus": [16480.1], "XYStage": [ 48764, -10919.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos28", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.42000000000002], "Focus": [16480.2], "XYStage": [ 43978.4, -10920.400000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos29", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.435], "Focus": [16480.2], "XYStage": [ 43106.3, -10920.400000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos30", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.405], "Focus": [16480.3], "XYStage": [ 38212.4, -10920.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos31", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.445], "Focus": [16480.2], "XYStage": [ 37254.9, -10920.400000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos32", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.44], "Focus": [16480.3], "XYStage": [ 32472.800000000003, -11294.400000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos33", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [166.95000000000002], "Focus": [16480.2], "XYStage": [ 31420.7, -11294.400000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos34", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [166.95000000000002], "Focus": [16480.2], "XYStage": [ 26881.5, -11640 ] }, "GridColumnIndex": 0 }, { "Label": "Pos35", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [166.98], "Focus": [16480.2], "XYStage": [ 25876.100000000002, -11641.1 ] }, "GridColumnIndex": 0 } ], "z-step_um": 10, "GridRow": 0, "BitDepth": 16, "Depth": 2, "Channels": 6, "Source": "Micro-Manager", "ChColors": [ -16711681, -256, -65281, -1, -1, -16776961 ], "MetadataVersion": 10, "KeepShutterOpenChannels": true, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536, 65536 ], "CustomIntervals_ms": [], "Height": 1200, "MicroManagerVersion": "1.4.23 20180219", "GridColumn": 0, "UUID": "8c8f9d20-b128-4577-9f91-89e70f22d319", "PixelType": "GRAY16", "Width": 1200, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\Yan\\20191217-YC113-S5S6", "Frames": 1, "Comment": "", "UserName": "Nikon", "PixelSize_um": 1.1, "ROI": [ 0, 0, 1200, 1200 ], "Date": "2019-12-17", "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC", "P-DAPI-high" ], "KeepShutterOpenSlices": true, "IJType": 1, "ComputerName": "ZadorConfocal2", "Positions": 36, "PositionIndex": 3, "CameraTimeout": 20000, "TimeFirst": false }, "640 AO-Name": "AnalogIO", "Vincent-D3-Command": "Close" }, "FrameKey-0-2-6": { "COM4-StopBits": "1", "528 AO-MaxVolts": "5.0000", "XLIGHT Excitation Wheel-State": "2", "470 Shutter-Name": "AnalogIO", "Core-Shutter": "640", "555 AO-Demo": "No", "XLIGHT Dichroic-State": "2", "XYStage-StepSize-Y": "0.1000", "ZPositionUm": 187.405, "XYStage-StepSize-X": "0.1000", "PRIME-FirmwareVersion": "20.28", "PRIME-ClearCycles": "2", "520-Name": "DA Shutter", "405 Shutter-Name": "AnalogIO", "PIEZO-StepSize": "0.0050", "PRIME-ClearMode": "Pre-Exposure", "470 AO-MinVolts": "0.0000", "405 AO-SupportsTriggering": "1", "CameraChannelIndex": 0, "XYStage-StartSpeed": "500.0000", "COM9-StopBits": "2", "405 AO-Description": "NI DAC", "COM4-DelayBetweenCharsMs": "0.0000", "445 AO-Block voltage": "false", "520 AO-TriggerInputLine": "/Dev1/PFI0", "555 AO-Description": "NI DAC", "520 AO-TriggerSampleRate": "100000", "COM4-Verbose": "1", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM4-Name": "COM4", "405 Shutter-Volts": "0.0000", "Vincent-D3-Port": "COM1", "PRIME-PP 4 MIN ADU AFFECTED": "200", "470 Shutter-MinVolts": "0.0000", "PRIME-PP 3 ENABLED": "Yes", "470 AO-Description": "NI DAC", "PRIME-Description": "PVCAM API device adapter", "520 Shutter-MaxVolts": "5.0000", "520 Shutter-Volts": "0.0000", "528 AO-Sequenceable": "1", "PRIME-Binning": "1x1", "555 Shutter-MaxVolts": "5.0000", "528 Shutter-TriggerSequenceLength": "1000", "405 Shutter-Block voltage": "false", "XLIGHT Spin motor-State": "1", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "Vincent-D3-Time to open (ms)": "35.0000", "Vincent-D3-Time to close (ms)": "35.0000", "445 AO-TriggerSampleRate": "100000", "640 Shutter-IOChannel": "Dev1/ao14", "555 AO-Sequenceable": "1", "520 AO-Name": "AnalogIO", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "470 AO-Block voltage": "false", "COM1-Verbose": "1", "COM9-AnswerTimeout": "10000.0000", "528 Shutter-Name": "AnalogIO", "PRIME-Trigger Timeout (secs)": "10", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "NextFrame": 0, "470 AO-TriggerSequenceLength": "1000", "PRIME-TransposeXY": "0", "445 AO-TriggerSequenceLength": "1000", "COM1-Name": "COM1", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "COM9-DataBits": "8", "COM1-AnswerTimeout": "500.0000", "Camera": "", "TE2000-Version": "V2.50-V2.50-V2.10", "528 Shutter-Volts": "0.0000", "XLIGHT Hub-Name": "XLIGHT Hub", "PIEZO-Description": "Ludl stage driver adapter", "PositionName": "Pos3", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSampleRate": "100000", "PRIME-FanSpeedSetpoint": "High", "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "405 AO-TriggerSampleRate": "100000", "470 Shutter-Block voltage": "false", "470-State": "0", "COM3-Parity": "None", "405 AO-Volts": "0.0000", "PRIME-TransposeMirrorY": "0", "PRIME-ChipName": "GS144BSI", "470 AO-Volts": "0.0000", "PRIME-TransposeMirrorX": "0", "445 Shutter-SupportsTriggering": "0", "470 Shutter-SupportsTriggering": "0", "XLIGHT Hub-Description": "XLIGHT hub", "PRIME-PreampOffLimit": "10000", "470 AO-Sequenceable": "1", "528 Shutter-MaxVolts": "5.0000", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "Core-XYStage": "XYStage", "470 AO-TriggerSampleRate": "100000", "PRIME-Timing-PreTriggerDelayNs": "24672000.0000", "528-DA Device": "528 Shutter", "Focus-Description": "Nikon TE2000 focus adapter", "FilterBlock-State": "5", "520 AO-Demo": "No", "COM3-DelayBetweenCharsMs": "0.0000", "470 Shutter-Volts": "0.0000", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "PRIME-PP 2 THRESHOLD": "125", "PRIME-PP 4 THRESHOLD": "75", "XYStage-Acceleration": "4.0000", "470 Shutter-Demo": "No", "PRIME-Name": "PMPCIECam00", "640-DA Device": "640 Shutter", "405 AO-Block voltage": "false", "ElapsedTime-ms": 147823, "PRIME-Offset": "100", "445 AO-IOChannel": "Dev1/ao1", "640 AO-MaxVolts": "5.0000", "PositionIndex": 3, "PRIME-PP 1 ENABLED": "Yes", "COM1-BaudRate": "9600", "640-State": "1", "COM3-Verbose": "1", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "LudlController-TransmissionDelay": "0", "PRIME-FullWellCapacity": "62000", "PRIME-SMARTStreamingEnabled": "No", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "640 Shutter-Description": "NI DAC", "528-Description": "DA device that is used as a shutter", "COM4-Description": "Serial port driver (boost:asio)", "405 Shutter-Description": "NI DAC", "TE2000-Name": "TE2000", "528 Shutter-Description": "NI DAC", "COM9-Description": "Serial port driver (boost:asio)", "LudlController-Name": "LudlController", "445 Shutter-TriggerSequenceLength": "1000", "PRIME-SerialNumber": "A18B203008", "445 AO-Volts": "0.0000", "PRIME-X-dimension": "1200", "PRIME-ShutterCloseDelay": "0", "XLIGHT Disk slider-State": "2", "Position": "Default", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 AO-IOChannel": "Dev1/ao5", "520 AO-IOChannel": "Dev1/ao4", "528 AO-TriggerSequenceLength": "1000", "405 Shutter-MaxVolts": "5.0000", "470-DA Device": "470 Shutter", "PRIME-FTCapable": "No", "Core-ChannelGroup": "Channels", "555 AO-SupportsTriggering": "0", "PRIME-Y-dimension": "1200", "Frame": 0, "555 Shutter-Sequenceable": "1", "PRIME-Timing-ExposureTimeNs": "9992000.0000", "Core-ImageProcessor": "", "528-State": "0", "520 AO-Block voltage": "false", "XLIGHT Excitation Wheel-Label": "QUAD", "640 Shutter-Demo": "No", "COM3-StopBits": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "520 Shutter-IOChannel": "Dev1/ao11", "Width": 1200, "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-SupportsTriggering": "0", "640 AO-TriggerSampleRate": "100000", "520-Description": "DA device that is used as a shutter", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "405 Shutter-Sequenceable": "1", "405 Shutter-SupportsTriggering": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-TriggerSampleRate": "100000", "640 Shutter-MaxVolts": "5.0000", "555 AO-MaxVolts": "5.0000", "520 AO-TriggerSequenceLength": "1000", "COM3-BaudRate": "9600", "COM9-BaudRate": "9600", "Vincent-D3-Channel #": "Ch. #1", "PRIME-PMode": "Normal", "OpticalPath-Label": "Left(100)", "BitDepth": 16, "555 AO-TriggerSequenceLength": "1000", "445 Shutter-Description": "NI DAC", "405 AO-Sequenceable": "1", "PRIME-AcquisitionMethod": "Callbacks", "555 AO-Name": "AnalogIO", "PRIME-ShutterMode": "Pre-Exposure", "COM3-Handshaking": "Off", "445 AO-SupportsTriggering": "0", "ROI": "0-0-1200-1200", "COM9-Verbose": "1", "PRIME-Color": "OFF", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "PRIME-ShutterOpenDelay": "0", "COM1-Parity": "None", "Slice": 6, "SlicePosition": 10, "COM9-DelayBetweenCharsMs": "0.0000", "555-Name": "DA Shutter", "PIEZO-LudlSingleAxisName": "B", "XLIGHT Emission Wheel-Label": "679/29 (A)", "640 Shutter-Name": "AnalogIO", "640 AO-Sequenceable": "1", "520 Shutter-Demo": "No", "528 Shutter-Demo": "No", "470-Name": "DA Shutter", "520-DA Device": "520 Shutter", "XYStage-Description": "Ludl XY stage driver adapter", "445 Shutter-MinVolts": "0.0000", "528 AO-TriggerSampleRate": "100000", "405 AO-MinVolts": "0.0000", "405 Shutter-TriggerSequenceLength": "1000", "Height": 1200, "555 AO-Block voltage": "false", "640 Shutter-Volts": "5.0000", "PixelType": "GRAY16", "445 Shutter-Block voltage": "false", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "520 Shutter-TriggerSequenceLength": "1000", "COM9-Name": "COM9", "Core-SLM": "", "555 AO-Volts": "0.0000", "555 Shutter-Block voltage": "false", "XYStage-Speed": "1800.0000", "520 AO-MaxVolts": "5.0000", "528 Shutter-SupportsTriggering": "0", "COM1-Description": "Serial port driver (boost:asio)", "PRIME-MetadataEnabled": "No", "520 Shutter-MinVolts": "0.0000", "470 Shutter-Sequenceable": "1", "PRIME-PreampDelay": "0", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-MinVolts": "0.0000", "ChannelIndex": 2, "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Core-AutoShutter": "1", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "Exposure-ms": 320, "PRIME-PP 2 ENABLED": "Yes", "640 Shutter-TriggerSampleRate": "100000", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "470 Shutter-IOChannel": "Dev1/ao10", "405 AO-Demo": "No", "PixelSizeUm": 1.1, "470 AO-Demo": "No", "470 Shutter-MaxVolts": "5.0000", "Vincent-D3-Name": "Vincent-D3", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FileName": "img_000000000_Il-A_006.tif", "LudlController-Reset": "Operate", "PRIME-Exposure": "320.00", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "445-Name": "DA Shutter", "555 Shutter-Name": "AnalogIO", "520 Shutter-TriggerSampleRate": "100000", "445 Shutter-IOChannel": "Dev1/ao9", "PRIME-TransposeCorrection": "0", "XYStage-StepSize": "0.1000", "445-Description": "DA device that is used as a shutter", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 AO-IOChannel": "Dev1/ao7", "520 Shutter-Name": "AnalogIO", "COM3-DataBits": "8", "640 AO-Volts": "4.5000", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "555 Shutter-IOChannel": "Dev1/ao13", "WaitInterval": null, "520 Shutter-Sequenceable": "1", "YPositionUm": 20690.300000000003, "445-State": "0", "445-DA Device": "445 Shutter", "PRIME-CircularBufferAutoSize": "ON", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "555 AO-TriggerSampleRate": "100000", "445 Shutter-Volts": "0.0000", "XLIGHT Hub-Port": "COM3", "Core-AutoFocus": "", "COM4-Handshaking": "Off", "528 Shutter-MinVolts": "0.0000", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "445 Shutter-Name": "AnalogIO", "528 Shutter-Sequenceable": "1", "XYStage-TransposeMirrorX": "0", "XYStage-TransposeMirrorY": "0", "PRIME-ReadoutRate": "100MHz 16bit", "XYStage-Name": "Stage", "640 AO-SupportsTriggering": "0", "640-Name": "DA Shutter", "640 Shutter-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "640 AO-Block voltage": "false", "445 Shutter-Sequenceable": "1", "470 AO-MaxVolts": "5.0000", "COM4-BaudRate": "9600", "470-Description": "DA device that is used as a shutter", "520 Shutter-Description": "NI DAC", "PRIME-CCDTemperature": "-14.9600", "Time": "2019-12-17 21:58:55 -0500", "640 AO-TriggerSequenceLength": "1000", "470 AO-Name": "AnalogIO", "XLIGHT Disk slider-Label": "Disk Pos 40um", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "Binning": "1x1", "TE2000-ModelType": "1", "COM3-AnswerTimeout": "500.0000", "555 Shutter-SupportsTriggering": "0", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "TE2000-Port": "COM4", "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Description": "NI DAC", "COM4-AnswerTimeout": "500.0000", "COM1-DelayBetweenCharsMs": "0.0000", "FilterBlock-Label": "Confocal", "445 AO-Name": "AnalogIO", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-Timing-ReadoutTimeNs": "24672000.0000", "528 AO-Demo": "No", "520 AO-Sequenceable": "1", "555-Description": "DA device that is used as a shutter", "405-DA Device": "405 Shutter", "405 AO-TriggerSequenceLength": "1000", "640 AO-MinVolts": "0.0000", "640 Shutter-Block voltage": "false", "FrameIndex": 0, "PRIME-Port": "CMOS", "555 AO-MinVolts": "0.0000", "COM3-Description": "Serial port driver (boost:asio)", "528 AO-Description": "NI DAC", "555 AO-IOChannel": "Dev1/ao6", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "555 AO-TriggerInputLine": "/Dev1/PFI0", "PRIME-ActualInterval-ms": "374.3700", "LudlController-Port": "COM9", "445 Shutter-Demo": "No", "PRIME-Trigger-Expose Out-Mux": "1", "SliceIndex": 6, "PRIME-CCDTemperatureSetPoint": "-15.0000", "405 Shutter-MinVolts": "0.0000", "PRIME-ExposeOutMode": "First Row", "AxisPositions": null, "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Volts": "0.0000", "528-Name": "DA Shutter", "555-State": "0", "XLIGHT Dichroic-Label": "Di03-R635-t1", "445 AO-Sequenceable": "1", "405-Name": "DA Shutter", "405 AO-IOChannel": "Dev1/ao0", "Core-Camera": "PRIME", "Focus-Name": "Focus", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "405 Shutter-IOChannel": "Dev1/ao8", "640 AO-Demo": "No", "640 Shutter-SupportsTriggering": "0", "445 AO-Demo": "No", "555-DA Device": "555 Shutter", "470 Shutter-Description": "NI DAC", "528 AO-Volts": "0.0000", "COM1-DataBits": "8", "Vincent-D3-Address": "x", "520 AO-SupportsTriggering": "0", "405-Description": "DA device that is used as a shutter", "640 Shutter-MinVolts": "0.0000", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "405 AO-Name": "AnalogIO", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "640 AO-TriggerInputLine": "/Dev1/PFI0", "405 AO-TriggerInputLine": "/Dev1/PFI0", "640-Description": "DA device that is used as a shutter", "445 Shutter-TriggerSampleRate": "100000", "470 Shutter-TriggerSequenceLength": "1000", "COM1-StopBits": "1", "520 Shutter-SupportsTriggering": "0", "405-State": "0", "Core-Initialize": "1", "XLIGHT Emission Wheel-State": "7", "520 AO-Description": "NI DAC", "528 AO-TriggerInputLine": "/Dev1/PFI0", "445 Shutter-MaxVolts": "5.0000", "COM9-Handshaking": "Off", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "XLIGHT Spin motor-Label": "On (spinning)", "PRIME-PP 5 ENABLED": "No", "UUID": "5a79a115-e357-496d-b4bb-6a51f453e926", "PRIME-TriggerMode": "Internal Trigger", "COM1-Handshaking": "Off", "PRIME-Timing-PostTriggerDelayNs": "49344000.0000", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "Focus-Position": "16479.8000", "528 Shutter-Block voltage": "false", "Channel": "Il-A", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "555 Shutter-Demo": "No", "405 Shutter-Demo": "No", "528 AO-Name": "AnalogIO", "520-State": "0", "Core-Galvo": "", "405 Shutter-TriggerSampleRate": "100000", "Focus-FocusStepSize_(nm)": "100", "PRIME-PixelType": "16bit", "COM4-DataBits": "8", "470 AO-IOChannel": "Dev1/ao2", "520 AO-MinVolts": "0.0000", "COM9-Parity": "None", "PRIME-CircularBufferEnabled": "ON", "PRIME-PP 6 Reset": "No", "640 AO-Description": "NI DAC", "Source": "PRIME", "445 AO-MinVolts": "0.0000", "PIEZO-Name": "Stage", "445 AO-Description": "NI DAC", "PRIME-CircularBufferFrameCount": "46", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "520 AO-Volts": "0.0000", "528 Shutter-TriggerSampleRate": "100000", "XPositionUm": 62321, "OpticalPath-State": "4", "PRIME-PP 3 THRESHOLD": "80", "PRIME-PVCAM Version": "3.7.1", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Gain": "1-HDR", "640 Shutter-TriggerSequenceLength": "1000", "528 AO-SupportsTriggering": "0", "445 AO-TriggerInputLine": "/Dev1/PFI0", "OpticalPath-Name": "OpticalPath", "PRIME-PP 4 ENABLED": "Yes", "PRIME-PVCAM Adapter Version": "1.3.29", "COM3-Name": "COM3", "405 AO-MaxVolts": "5.0000", "520 Shutter-Block voltage": "false", "PRIME-Timing-ClearingTimeNs": "49344000.0000", "Core-Focus": "PIEZO", "528 AO-Block voltage": "false", "TE2000-Description": "Nikon TE2000 hub", "528 AO-MinVolts": "0.0000", "COM4-Parity": "None", "Summary": { "Slices": 11, "ChContrastMin": [ 0, 0, 0, 0, 0, 0 ], "PixelAspect": 1, "SlicesFirst": true, "Interval_ms": 0, "Time": "2019-12-17 21:58:39 -0500", "Prefix": "seq_3", "InitialPositionList": [ { "Label": "Pos0", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.435], "Focus": [16480.2], "XYStage": [ 69822.40000000001, 20100.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos1", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.425], "Focus": [16480.3], "XYStage": [ 69004, 20101 ] }, "GridColumnIndex": 0 }, { "Label": "Pos2", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.44], "Focus": [16480.1], "XYStage": [ 63119.5, 20690.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos3", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.405], "Focus": [16480.2], "XYStage": [ 62321, 20690.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos4", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.415], "Focus": [16480], "XYStage": [ 56604.100000000006, 20688.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos5", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.42000000000002], "Focus": [16480.1], "XYStage": [ 56034.200000000004, 20688.800000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos6", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.455], "Focus": [16480], "XYStage": [ 50889.3, 20496.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos7", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.43], "Focus": [16480.2], "XYStage": [ 50101.600000000006, 20496.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos8", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.67000000000002], "Focus": [16479.9], "XYStage": [ 44818.600000000006, 20496.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos9", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.67000000000002], "Focus": [16480], "XYStage": [ 43897.9, 20436.800000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos10", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.675], "Focus": [16479.9], "XYStage": [ 39670.600000000006, 20664.9 ] }, "GridColumnIndex": 0 }, { "Label": "Pos11", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.69], "Focus": [16479.9], "XYStage": [ 38734, 20664.600000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos12", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.695], "Focus": [16480.1], "XYStage": [ 34037.200000000004, 20664.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos13", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.685], "Focus": [16480.1], "XYStage": [ 33152, 20664.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos14", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.69], "Focus": [16480.1], "XYStage": [ 28122.800000000003, 20664.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos15", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.675], "Focus": [16480], "XYStage": [ 27422.9, 20664.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos16", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.64000000000001], "Focus": [16480], "XYStage": [ 23005.2, 20664.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos17", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.69], "Focus": [16479.9], "XYStage": [ 21820.600000000002, 20409.600000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos18", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.41], "Focus": [16480.1], "XYStage": [ 73697.6, -13222.800000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos19", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.455], "Focus": [16480.1], "XYStage": [ 72765.8, -13223 ] }, "GridColumnIndex": 0 }, { "Label": "Pos20", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.4], "Focus": [16480.2], "XYStage": [ 67445.5, -12303.800000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos21", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.415], "Focus": [16480.2], "XYStage": [ 66613.3, -12303.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos22", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.415], "Focus": [16480.1], "XYStage": [ 61819.4, -11936.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos23", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.405], "Focus": [16480.2], "XYStage": [ 61325.700000000004, -11937.1 ] }, "GridColumnIndex": 0 }, { "Label": "Pos24", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.46], "Focus": [16480.2], "XYStage": [ 55377, -10974.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos25", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.445], "Focus": [16480.1], "XYStage": [ 54827, -11214.800000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos26", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.435], "Focus": [16480.2], "XYStage": [ 49937.100000000006, -10919.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos27", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.425], "Focus": [16480.1], "XYStage": [ 48764, -10919.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos28", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.42000000000002], "Focus": [16480.2], "XYStage": [ 43978.4, -10920.400000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos29", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.435], "Focus": [16480.2], "XYStage": [ 43106.3, -10920.400000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos30", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.405], "Focus": [16480.3], "XYStage": [ 38212.4, -10920.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos31", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.445], "Focus": [16480.2], "XYStage": [ 37254.9, -10920.400000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos32", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.44], "Focus": [16480.3], "XYStage": [ 32472.800000000003, -11294.400000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos33", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [166.95000000000002], "Focus": [16480.2], "XYStage": [ 31420.7, -11294.400000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos34", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [166.95000000000002], "Focus": [16480.2], "XYStage": [ 26881.5, -11640 ] }, "GridColumnIndex": 0 }, { "Label": "Pos35", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [166.98], "Focus": [16480.2], "XYStage": [ 25876.100000000002, -11641.1 ] }, "GridColumnIndex": 0 } ], "z-step_um": 10, "GridRow": 0, "BitDepth": 16, "Depth": 2, "Channels": 6, "Source": "Micro-Manager", "ChColors": [ -16711681, -256, -65281, -1, -1, -16776961 ], "MetadataVersion": 10, "KeepShutterOpenChannels": true, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536, 65536 ], "CustomIntervals_ms": [], "Height": 1200, "MicroManagerVersion": "1.4.23 20180219", "GridColumn": 0, "UUID": "8c8f9d20-b128-4577-9f91-89e70f22d319", "PixelType": "GRAY16", "Width": 1200, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\Yan\\20191217-YC113-S5S6", "Frames": 1, "Comment": "", "UserName": "Nikon", "PixelSize_um": 1.1, "ROI": [ 0, 0, 1200, 1200 ], "Date": "2019-12-17", "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC", "P-DAPI-high" ], "KeepShutterOpenSlices": true, "IJType": 1, "ComputerName": "ZadorConfocal2", "Positions": 36, "PositionIndex": 3, "CameraTimeout": 20000, "TimeFirst": false }, "640 AO-Name": "AnalogIO", "Vincent-D3-Command": "Close" }, "FrameKey-0-2-7": { "COM4-StopBits": "1", "528 AO-MaxVolts": "5.0000", "XLIGHT Excitation Wheel-State": "2", "470 Shutter-Name": "AnalogIO", "Core-Shutter": "640", "555 AO-Demo": "No", "XLIGHT Dichroic-State": "2", "XYStage-StepSize-Y": "0.1000", "ZPositionUm": 197.405, "XYStage-StepSize-X": "0.1000", "PRIME-FirmwareVersion": "20.28", "PRIME-ClearCycles": "2", "520-Name": "DA Shutter", "405 Shutter-Name": "AnalogIO", "PIEZO-StepSize": "0.0050", "PRIME-ClearMode": "Pre-Exposure", "470 AO-MinVolts": "0.0000", "405 AO-SupportsTriggering": "1", "CameraChannelIndex": 0, "XYStage-StartSpeed": "500.0000", "COM9-StopBits": "2", "405 AO-Description": "NI DAC", "COM4-DelayBetweenCharsMs": "0.0000", "445 AO-Block voltage": "false", "520 AO-TriggerInputLine": "/Dev1/PFI0", "555 AO-Description": "NI DAC", "520 AO-TriggerSampleRate": "100000", "COM4-Verbose": "1", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM4-Name": "COM4", "405 Shutter-Volts": "0.0000", "Vincent-D3-Port": "COM1", "PRIME-PP 4 MIN ADU AFFECTED": "200", "470 Shutter-MinVolts": "0.0000", "PRIME-PP 3 ENABLED": "Yes", "470 AO-Description": "NI DAC", "PRIME-Description": "PVCAM API device adapter", "520 Shutter-MaxVolts": "5.0000", "520 Shutter-Volts": "0.0000", "528 AO-Sequenceable": "1", "PRIME-Binning": "1x1", "555 Shutter-MaxVolts": "5.0000", "528 Shutter-TriggerSequenceLength": "1000", "405 Shutter-Block voltage": "false", "XLIGHT Spin motor-State": "1", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "Vincent-D3-Time to open (ms)": "35.0000", "Vincent-D3-Time to close (ms)": "35.0000", "445 AO-TriggerSampleRate": "100000", "640 Shutter-IOChannel": "Dev1/ao14", "555 AO-Sequenceable": "1", "520 AO-Name": "AnalogIO", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "470 AO-Block voltage": "false", "COM1-Verbose": "1", "COM9-AnswerTimeout": "10000.0000", "528 Shutter-Name": "AnalogIO", "PRIME-Trigger Timeout (secs)": "10", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "NextFrame": 0, "470 AO-TriggerSequenceLength": "1000", "PRIME-TransposeXY": "0", "445 AO-TriggerSequenceLength": "1000", "COM1-Name": "COM1", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "COM9-DataBits": "8", "COM1-AnswerTimeout": "500.0000", "Camera": "", "TE2000-Version": "V2.50-V2.50-V2.10", "528 Shutter-Volts": "0.0000", "XLIGHT Hub-Name": "XLIGHT Hub", "PIEZO-Description": "Ludl stage driver adapter", "PositionName": "Pos3", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSampleRate": "100000", "PRIME-FanSpeedSetpoint": "High", "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "405 AO-TriggerSampleRate": "100000", "470 Shutter-Block voltage": "false", "470-State": "0", "COM3-Parity": "None", "405 AO-Volts": "0.0000", "PRIME-TransposeMirrorY": "0", "PRIME-ChipName": "GS144BSI", "470 AO-Volts": "0.0000", "PRIME-TransposeMirrorX": "0", "445 Shutter-SupportsTriggering": "0", "470 Shutter-SupportsTriggering": "0", "XLIGHT Hub-Description": "XLIGHT hub", "PRIME-PreampOffLimit": "10000", "470 AO-Sequenceable": "1", "528 Shutter-MaxVolts": "5.0000", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "Core-XYStage": "XYStage", "470 AO-TriggerSampleRate": "100000", "PRIME-Timing-PreTriggerDelayNs": "24672000.0000", "528-DA Device": "528 Shutter", "Focus-Description": "Nikon TE2000 focus adapter", "FilterBlock-State": "5", "520 AO-Demo": "No", "COM3-DelayBetweenCharsMs": "0.0000", "470 Shutter-Volts": "0.0000", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "PRIME-PP 2 THRESHOLD": "125", "PRIME-PP 4 THRESHOLD": "75", "XYStage-Acceleration": "4.0000", "470 Shutter-Demo": "No", "PRIME-Name": "PMPCIECam00", "640-DA Device": "640 Shutter", "405 AO-Block voltage": "false", "ElapsedTime-ms": 148384, "PRIME-Offset": "100", "445 AO-IOChannel": "Dev1/ao1", "640 AO-MaxVolts": "5.0000", "PositionIndex": 3, "PRIME-PP 1 ENABLED": "Yes", "COM1-BaudRate": "9600", "640-State": "1", "COM3-Verbose": "1", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "LudlController-TransmissionDelay": "0", "PRIME-FullWellCapacity": "62000", "PRIME-SMARTStreamingEnabled": "No", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "640 Shutter-Description": "NI DAC", "528-Description": "DA device that is used as a shutter", "COM4-Description": "Serial port driver (boost:asio)", "405 Shutter-Description": "NI DAC", "TE2000-Name": "TE2000", "528 Shutter-Description": "NI DAC", "COM9-Description": "Serial port driver (boost:asio)", "LudlController-Name": "LudlController", "445 Shutter-TriggerSequenceLength": "1000", "PRIME-SerialNumber": "A18B203008", "445 AO-Volts": "0.0000", "PRIME-X-dimension": "1200", "PRIME-ShutterCloseDelay": "0", "XLIGHT Disk slider-State": "2", "Position": "Default", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 AO-IOChannel": "Dev1/ao5", "520 AO-IOChannel": "Dev1/ao4", "528 AO-TriggerSequenceLength": "1000", "405 Shutter-MaxVolts": "5.0000", "470-DA Device": "470 Shutter", "PRIME-FTCapable": "No", "Core-ChannelGroup": "Channels", "555 AO-SupportsTriggering": "0", "PRIME-Y-dimension": "1200", "Frame": 0, "555 Shutter-Sequenceable": "1", "PRIME-Timing-ExposureTimeNs": "9992000.0000", "Core-ImageProcessor": "", "528-State": "0", "520 AO-Block voltage": "false", "XLIGHT Excitation Wheel-Label": "QUAD", "640 Shutter-Demo": "No", "COM3-StopBits": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "520 Shutter-IOChannel": "Dev1/ao11", "Width": 1200, "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-SupportsTriggering": "0", "640 AO-TriggerSampleRate": "100000", "520-Description": "DA device that is used as a shutter", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "405 Shutter-Sequenceable": "1", "405 Shutter-SupportsTriggering": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-TriggerSampleRate": "100000", "640 Shutter-MaxVolts": "5.0000", "555 AO-MaxVolts": "5.0000", "520 AO-TriggerSequenceLength": "1000", "COM3-BaudRate": "9600", "COM9-BaudRate": "9600", "Vincent-D3-Channel #": "Ch. #1", "PRIME-PMode": "Normal", "OpticalPath-Label": "Left(100)", "BitDepth": 16, "555 AO-TriggerSequenceLength": "1000", "445 Shutter-Description": "NI DAC", "405 AO-Sequenceable": "1", "PRIME-AcquisitionMethod": "Callbacks", "555 AO-Name": "AnalogIO", "PRIME-ShutterMode": "Pre-Exposure", "COM3-Handshaking": "Off", "445 AO-SupportsTriggering": "0", "ROI": "0-0-1200-1200", "COM9-Verbose": "1", "PRIME-Color": "OFF", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "PRIME-ShutterOpenDelay": "0", "COM1-Parity": "None", "Slice": 7, "SlicePosition": 20, "COM9-DelayBetweenCharsMs": "0.0000", "555-Name": "DA Shutter", "PIEZO-LudlSingleAxisName": "B", "XLIGHT Emission Wheel-Label": "679/29 (A)", "640 Shutter-Name": "AnalogIO", "640 AO-Sequenceable": "1", "520 Shutter-Demo": "No", "528 Shutter-Demo": "No", "470-Name": "DA Shutter", "520-DA Device": "520 Shutter", "XYStage-Description": "Ludl XY stage driver adapter", "445 Shutter-MinVolts": "0.0000", "528 AO-TriggerSampleRate": "100000", "405 AO-MinVolts": "0.0000", "405 Shutter-TriggerSequenceLength": "1000", "Height": 1200, "555 AO-Block voltage": "false", "640 Shutter-Volts": "5.0000", "PixelType": "GRAY16", "445 Shutter-Block voltage": "false", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "520 Shutter-TriggerSequenceLength": "1000", "COM9-Name": "COM9", "Core-SLM": "", "555 AO-Volts": "0.0000", "555 Shutter-Block voltage": "false", "XYStage-Speed": "1800.0000", "520 AO-MaxVolts": "5.0000", "528 Shutter-SupportsTriggering": "0", "COM1-Description": "Serial port driver (boost:asio)", "PRIME-MetadataEnabled": "No", "520 Shutter-MinVolts": "0.0000", "470 Shutter-Sequenceable": "1", "PRIME-PreampDelay": "0", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-MinVolts": "0.0000", "ChannelIndex": 2, "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Core-AutoShutter": "1", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "Exposure-ms": 320, "PRIME-PP 2 ENABLED": "Yes", "640 Shutter-TriggerSampleRate": "100000", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "470 Shutter-IOChannel": "Dev1/ao10", "405 AO-Demo": "No", "PixelSizeUm": 1.1, "470 AO-Demo": "No", "470 Shutter-MaxVolts": "5.0000", "Vincent-D3-Name": "Vincent-D3", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FileName": "img_000000000_Il-A_007.tif", "LudlController-Reset": "Operate", "PRIME-Exposure": "320.00", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "445-Name": "DA Shutter", "555 Shutter-Name": "AnalogIO", "520 Shutter-TriggerSampleRate": "100000", "445 Shutter-IOChannel": "Dev1/ao9", "PRIME-TransposeCorrection": "0", "XYStage-StepSize": "0.1000", "445-Description": "DA device that is used as a shutter", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 AO-IOChannel": "Dev1/ao7", "520 Shutter-Name": "AnalogIO", "COM3-DataBits": "8", "640 AO-Volts": "4.5000", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "555 Shutter-IOChannel": "Dev1/ao13", "WaitInterval": null, "520 Shutter-Sequenceable": "1", "YPositionUm": 20690.300000000003, "445-State": "0", "445-DA Device": "445 Shutter", "PRIME-CircularBufferAutoSize": "ON", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "555 AO-TriggerSampleRate": "100000", "445 Shutter-Volts": "0.0000", "XLIGHT Hub-Port": "COM3", "Core-AutoFocus": "", "COM4-Handshaking": "Off", "528 Shutter-MinVolts": "0.0000", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "445 Shutter-Name": "AnalogIO", "528 Shutter-Sequenceable": "1", "XYStage-TransposeMirrorX": "0", "XYStage-TransposeMirrorY": "0", "PRIME-ReadoutRate": "100MHz 16bit", "XYStage-Name": "Stage", "640 AO-SupportsTriggering": "0", "640-Name": "DA Shutter", "640 Shutter-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "640 AO-Block voltage": "false", "445 Shutter-Sequenceable": "1", "470 AO-MaxVolts": "5.0000", "COM4-BaudRate": "9600", "470-Description": "DA device that is used as a shutter", "520 Shutter-Description": "NI DAC", "PRIME-CCDTemperature": "-14.9600", "Time": "2019-12-17 21:58:55 -0500", "640 AO-TriggerSequenceLength": "1000", "470 AO-Name": "AnalogIO", "XLIGHT Disk slider-Label": "Disk Pos 40um", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "Binning": "1x1", "TE2000-ModelType": "1", "COM3-AnswerTimeout": "500.0000", "555 Shutter-SupportsTriggering": "0", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "TE2000-Port": "COM4", "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Description": "NI DAC", "COM4-AnswerTimeout": "500.0000", "COM1-DelayBetweenCharsMs": "0.0000", "FilterBlock-Label": "Confocal", "445 AO-Name": "AnalogIO", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-Timing-ReadoutTimeNs": "24672000.0000", "528 AO-Demo": "No", "520 AO-Sequenceable": "1", "555-Description": "DA device that is used as a shutter", "405-DA Device": "405 Shutter", "405 AO-TriggerSequenceLength": "1000", "640 AO-MinVolts": "0.0000", "640 Shutter-Block voltage": "false", "FrameIndex": 0, "PRIME-Port": "CMOS", "555 AO-MinVolts": "0.0000", "COM3-Description": "Serial port driver (boost:asio)", "528 AO-Description": "NI DAC", "555 AO-IOChannel": "Dev1/ao6", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "555 AO-TriggerInputLine": "/Dev1/PFI0", "PRIME-ActualInterval-ms": "374.3700", "LudlController-Port": "COM9", "445 Shutter-Demo": "No", "PRIME-Trigger-Expose Out-Mux": "1", "SliceIndex": 7, "PRIME-CCDTemperatureSetPoint": "-15.0000", "405 Shutter-MinVolts": "0.0000", "PRIME-ExposeOutMode": "First Row", "AxisPositions": null, "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Volts": "0.0000", "528-Name": "DA Shutter", "555-State": "0", "XLIGHT Dichroic-Label": "Di03-R635-t1", "445 AO-Sequenceable": "1", "405-Name": "DA Shutter", "405 AO-IOChannel": "Dev1/ao0", "Core-Camera": "PRIME", "Focus-Name": "Focus", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "405 Shutter-IOChannel": "Dev1/ao8", "640 AO-Demo": "No", "640 Shutter-SupportsTriggering": "0", "445 AO-Demo": "No", "555-DA Device": "555 Shutter", "470 Shutter-Description": "NI DAC", "528 AO-Volts": "0.0000", "COM1-DataBits": "8", "Vincent-D3-Address": "x", "520 AO-SupportsTriggering": "0", "405-Description": "DA device that is used as a shutter", "640 Shutter-MinVolts": "0.0000", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "405 AO-Name": "AnalogIO", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "640 AO-TriggerInputLine": "/Dev1/PFI0", "405 AO-TriggerInputLine": "/Dev1/PFI0", "640-Description": "DA device that is used as a shutter", "445 Shutter-TriggerSampleRate": "100000", "470 Shutter-TriggerSequenceLength": "1000", "COM1-StopBits": "1", "520 Shutter-SupportsTriggering": "0", "405-State": "0", "Core-Initialize": "1", "XLIGHT Emission Wheel-State": "7", "520 AO-Description": "NI DAC", "528 AO-TriggerInputLine": "/Dev1/PFI0", "445 Shutter-MaxVolts": "5.0000", "COM9-Handshaking": "Off", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "XLIGHT Spin motor-Label": "On (spinning)", "PRIME-PP 5 ENABLED": "No", "UUID": "66112bfd-ac83-423e-bd6e-befbe0c80b16", "PRIME-TriggerMode": "Internal Trigger", "COM1-Handshaking": "Off", "PRIME-Timing-PostTriggerDelayNs": "49344000.0000", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "Focus-Position": "16479.8000", "528 Shutter-Block voltage": "false", "Channel": "Il-A", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "555 Shutter-Demo": "No", "405 Shutter-Demo": "No", "528 AO-Name": "AnalogIO", "520-State": "0", "Core-Galvo": "", "405 Shutter-TriggerSampleRate": "100000", "Focus-FocusStepSize_(nm)": "100", "PRIME-PixelType": "16bit", "COM4-DataBits": "8", "470 AO-IOChannel": "Dev1/ao2", "520 AO-MinVolts": "0.0000", "COM9-Parity": "None", "PRIME-CircularBufferEnabled": "ON", "PRIME-PP 6 Reset": "No", "640 AO-Description": "NI DAC", "Source": "PRIME", "445 AO-MinVolts": "0.0000", "PIEZO-Name": "Stage", "445 AO-Description": "NI DAC", "PRIME-CircularBufferFrameCount": "46", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "520 AO-Volts": "0.0000", "528 Shutter-TriggerSampleRate": "100000", "XPositionUm": 62321, "OpticalPath-State": "4", "PRIME-PP 3 THRESHOLD": "80", "PRIME-PVCAM Version": "3.7.1", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Gain": "1-HDR", "640 Shutter-TriggerSequenceLength": "1000", "528 AO-SupportsTriggering": "0", "445 AO-TriggerInputLine": "/Dev1/PFI0", "OpticalPath-Name": "OpticalPath", "PRIME-PP 4 ENABLED": "Yes", "PRIME-PVCAM Adapter Version": "1.3.29", "COM3-Name": "COM3", "405 AO-MaxVolts": "5.0000", "520 Shutter-Block voltage": "false", "PRIME-Timing-ClearingTimeNs": "49344000.0000", "Core-Focus": "PIEZO", "528 AO-Block voltage": "false", "TE2000-Description": "Nikon TE2000 hub", "528 AO-MinVolts": "0.0000", "COM4-Parity": "None", "Summary": { "Slices": 11, "ChContrastMin": [ 0, 0, 0, 0, 0, 0 ], "PixelAspect": 1, "SlicesFirst": true, "Interval_ms": 0, "Time": "2019-12-17 21:58:39 -0500", "Prefix": "seq_3", "InitialPositionList": [ { "Label": "Pos0", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.435], "Focus": [16480.2], "XYStage": [ 69822.40000000001, 20100.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos1", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.425], "Focus": [16480.3], "XYStage": [ 69004, 20101 ] }, "GridColumnIndex": 0 }, { "Label": "Pos2", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.44], "Focus": [16480.1], "XYStage": [ 63119.5, 20690.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos3", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.405], "Focus": [16480.2], "XYStage": [ 62321, 20690.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos4", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.415], "Focus": [16480], "XYStage": [ 56604.100000000006, 20688.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos5", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.42000000000002], "Focus": [16480.1], "XYStage": [ 56034.200000000004, 20688.800000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos6", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.455], "Focus": [16480], "XYStage": [ 50889.3, 20496.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos7", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.43], "Focus": [16480.2], "XYStage": [ 50101.600000000006, 20496.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos8", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.67000000000002], "Focus": [16479.9], "XYStage": [ 44818.600000000006, 20496.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos9", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.67000000000002], "Focus": [16480], "XYStage": [ 43897.9, 20436.800000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos10", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.675], "Focus": [16479.9], "XYStage": [ 39670.600000000006, 20664.9 ] }, "GridColumnIndex": 0 }, { "Label": "Pos11", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.69], "Focus": [16479.9], "XYStage": [ 38734, 20664.600000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos12", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.695], "Focus": [16480.1], "XYStage": [ 34037.200000000004, 20664.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos13", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.685], "Focus": [16480.1], "XYStage": [ 33152, 20664.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos14", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.69], "Focus": [16480.1], "XYStage": [ 28122.800000000003, 20664.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos15", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.675], "Focus": [16480], "XYStage": [ 27422.9, 20664.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos16", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.64000000000001], "Focus": [16480], "XYStage": [ 23005.2, 20664.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos17", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.69], "Focus": [16479.9], "XYStage": [ 21820.600000000002, 20409.600000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos18", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.41], "Focus": [16480.1], "XYStage": [ 73697.6, -13222.800000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos19", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.455], "Focus": [16480.1], "XYStage": [ 72765.8, -13223 ] }, "GridColumnIndex": 0 }, { "Label": "Pos20", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.4], "Focus": [16480.2], "XYStage": [ 67445.5, -12303.800000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos21", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.415], "Focus": [16480.2], "XYStage": [ 66613.3, -12303.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos22", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.415], "Focus": [16480.1], "XYStage": [ 61819.4, -11936.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos23", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.405], "Focus": [16480.2], "XYStage": [ 61325.700000000004, -11937.1 ] }, "GridColumnIndex": 0 }, { "Label": "Pos24", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.46], "Focus": [16480.2], "XYStage": [ 55377, -10974.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos25", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.445], "Focus": [16480.1], "XYStage": [ 54827, -11214.800000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos26", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.435], "Focus": [16480.2], "XYStage": [ 49937.100000000006, -10919.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos27", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.425], "Focus": [16480.1], "XYStage": [ 48764, -10919.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos28", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.42000000000002], "Focus": [16480.2], "XYStage": [ 43978.4, -10920.400000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos29", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.435], "Focus": [16480.2], "XYStage": [ 43106.3, -10920.400000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos30", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.405], "Focus": [16480.3], "XYStage": [ 38212.4, -10920.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos31", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.445], "Focus": [16480.2], "XYStage": [ 37254.9, -10920.400000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos32", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.44], "Focus": [16480.3], "XYStage": [ 32472.800000000003, -11294.400000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos33", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [166.95000000000002], "Focus": [16480.2], "XYStage": [ 31420.7, -11294.400000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos34", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [166.95000000000002], "Focus": [16480.2], "XYStage": [ 26881.5, -11640 ] }, "GridColumnIndex": 0 }, { "Label": "Pos35", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [166.98], "Focus": [16480.2], "XYStage": [ 25876.100000000002, -11641.1 ] }, "GridColumnIndex": 0 } ], "z-step_um": 10, "GridRow": 0, "BitDepth": 16, "Depth": 2, "Channels": 6, "Source": "Micro-Manager", "ChColors": [ -16711681, -256, -65281, -1, -1, -16776961 ], "MetadataVersion": 10, "KeepShutterOpenChannels": true, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536, 65536 ], "CustomIntervals_ms": [], "Height": 1200, "MicroManagerVersion": "1.4.23 20180219", "GridColumn": 0, "UUID": "8c8f9d20-b128-4577-9f91-89e70f22d319", "PixelType": "GRAY16", "Width": 1200, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\Yan\\20191217-YC113-S5S6", "Frames": 1, "Comment": "", "UserName": "Nikon", "PixelSize_um": 1.1, "ROI": [ 0, 0, 1200, 1200 ], "Date": "2019-12-17", "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC", "P-DAPI-high" ], "KeepShutterOpenSlices": true, "IJType": 1, "ComputerName": "ZadorConfocal2", "Positions": 36, "PositionIndex": 3, "CameraTimeout": 20000, "TimeFirst": false }, "640 AO-Name": "AnalogIO", "Vincent-D3-Command": "Close" }, "FrameKey-0-2-8": { "COM4-StopBits": "1", "528 AO-MaxVolts": "5.0000", "XLIGHT Excitation Wheel-State": "2", "470 Shutter-Name": "AnalogIO", "Core-Shutter": "640", "555 AO-Demo": "No", "XLIGHT Dichroic-State": "2", "XYStage-StepSize-Y": "0.1000", "ZPositionUm": 207.405, "XYStage-StepSize-X": "0.1000", "PRIME-FirmwareVersion": "20.28", "PRIME-ClearCycles": "2", "520-Name": "DA Shutter", "405 Shutter-Name": "AnalogIO", "PIEZO-StepSize": "0.0050", "PRIME-ClearMode": "Pre-Exposure", "470 AO-MinVolts": "0.0000", "405 AO-SupportsTriggering": "1", "CameraChannelIndex": 0, "XYStage-StartSpeed": "500.0000", "COM9-StopBits": "2", "405 AO-Description": "NI DAC", "COM4-DelayBetweenCharsMs": "0.0000", "445 AO-Block voltage": "false", "520 AO-TriggerInputLine": "/Dev1/PFI0", "555 AO-Description": "NI DAC", "520 AO-TriggerSampleRate": "100000", "COM4-Verbose": "1", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM4-Name": "COM4", "405 Shutter-Volts": "0.0000", "Vincent-D3-Port": "COM1", "PRIME-PP 4 MIN ADU AFFECTED": "200", "470 Shutter-MinVolts": "0.0000", "PRIME-PP 3 ENABLED": "Yes", "470 AO-Description": "NI DAC", "PRIME-Description": "PVCAM API device adapter", "520 Shutter-MaxVolts": "5.0000", "520 Shutter-Volts": "0.0000", "528 AO-Sequenceable": "1", "PRIME-Binning": "1x1", "555 Shutter-MaxVolts": "5.0000", "528 Shutter-TriggerSequenceLength": "1000", "405 Shutter-Block voltage": "false", "XLIGHT Spin motor-State": "1", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "Vincent-D3-Time to open (ms)": "35.0000", "Vincent-D3-Time to close (ms)": "35.0000", "445 AO-TriggerSampleRate": "100000", "640 Shutter-IOChannel": "Dev1/ao14", "555 AO-Sequenceable": "1", "520 AO-Name": "AnalogIO", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "470 AO-Block voltage": "false", "COM1-Verbose": "1", "COM9-AnswerTimeout": "10000.0000", "528 Shutter-Name": "AnalogIO", "PRIME-Trigger Timeout (secs)": "10", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "NextFrame": 0, "470 AO-TriggerSequenceLength": "1000", "PRIME-TransposeXY": "0", "445 AO-TriggerSequenceLength": "1000", "COM1-Name": "COM1", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "COM9-DataBits": "8", "COM1-AnswerTimeout": "500.0000", "Camera": "", "TE2000-Version": "V2.50-V2.50-V2.10", "528 Shutter-Volts": "0.0000", "XLIGHT Hub-Name": "XLIGHT Hub", "PIEZO-Description": "Ludl stage driver adapter", "PositionName": "Pos3", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSampleRate": "100000", "PRIME-FanSpeedSetpoint": "High", "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "405 AO-TriggerSampleRate": "100000", "470 Shutter-Block voltage": "false", "470-State": "0", "COM3-Parity": "None", "405 AO-Volts": "0.0000", "PRIME-TransposeMirrorY": "0", "PRIME-ChipName": "GS144BSI", "470 AO-Volts": "0.0000", "PRIME-TransposeMirrorX": "0", "445 Shutter-SupportsTriggering": "0", "470 Shutter-SupportsTriggering": "0", "XLIGHT Hub-Description": "XLIGHT hub", "PRIME-PreampOffLimit": "10000", "470 AO-Sequenceable": "1", "528 Shutter-MaxVolts": "5.0000", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "Core-XYStage": "XYStage", "470 AO-TriggerSampleRate": "100000", "PRIME-Timing-PreTriggerDelayNs": "24672000.0000", "528-DA Device": "528 Shutter", "Focus-Description": "Nikon TE2000 focus adapter", "FilterBlock-State": "5", "520 AO-Demo": "No", "COM3-DelayBetweenCharsMs": "0.0000", "470 Shutter-Volts": "0.0000", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "PRIME-PP 2 THRESHOLD": "125", "PRIME-PP 4 THRESHOLD": "75", "XYStage-Acceleration": "4.0000", "470 Shutter-Demo": "No", "PRIME-Name": "PMPCIECam00", "640-DA Device": "640 Shutter", "405 AO-Block voltage": "false", "ElapsedTime-ms": 148949, "PRIME-Offset": "100", "445 AO-IOChannel": "Dev1/ao1", "640 AO-MaxVolts": "5.0000", "PositionIndex": 3, "PRIME-PP 1 ENABLED": "Yes", "COM1-BaudRate": "9600", "640-State": "1", "COM3-Verbose": "1", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "LudlController-TransmissionDelay": "0", "PRIME-FullWellCapacity": "62000", "PRIME-SMARTStreamingEnabled": "No", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "640 Shutter-Description": "NI DAC", "528-Description": "DA device that is used as a shutter", "COM4-Description": "Serial port driver (boost:asio)", "405 Shutter-Description": "NI DAC", "TE2000-Name": "TE2000", "528 Shutter-Description": "NI DAC", "COM9-Description": "Serial port driver (boost:asio)", "LudlController-Name": "LudlController", "445 Shutter-TriggerSequenceLength": "1000", "PRIME-SerialNumber": "A18B203008", "445 AO-Volts": "0.0000", "PRIME-X-dimension": "1200", "PRIME-ShutterCloseDelay": "0", "XLIGHT Disk slider-State": "2", "Position": "Default", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 AO-IOChannel": "Dev1/ao5", "520 AO-IOChannel": "Dev1/ao4", "528 AO-TriggerSequenceLength": "1000", "405 Shutter-MaxVolts": "5.0000", "470-DA Device": "470 Shutter", "PRIME-FTCapable": "No", "Core-ChannelGroup": "Channels", "555 AO-SupportsTriggering": "0", "PRIME-Y-dimension": "1200", "Frame": 0, "555 Shutter-Sequenceable": "1", "PRIME-Timing-ExposureTimeNs": "9992000.0000", "Core-ImageProcessor": "", "528-State": "0", "520 AO-Block voltage": "false", "XLIGHT Excitation Wheel-Label": "QUAD", "640 Shutter-Demo": "No", "COM3-StopBits": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "520 Shutter-IOChannel": "Dev1/ao11", "Width": 1200, "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-SupportsTriggering": "0", "640 AO-TriggerSampleRate": "100000", "520-Description": "DA device that is used as a shutter", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "405 Shutter-Sequenceable": "1", "405 Shutter-SupportsTriggering": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-TriggerSampleRate": "100000", "640 Shutter-MaxVolts": "5.0000", "555 AO-MaxVolts": "5.0000", "520 AO-TriggerSequenceLength": "1000", "COM3-BaudRate": "9600", "COM9-BaudRate": "9600", "Vincent-D3-Channel #": "Ch. #1", "PRIME-PMode": "Normal", "OpticalPath-Label": "Left(100)", "BitDepth": 16, "555 AO-TriggerSequenceLength": "1000", "445 Shutter-Description": "NI DAC", "405 AO-Sequenceable": "1", "PRIME-AcquisitionMethod": "Callbacks", "555 AO-Name": "AnalogIO", "PRIME-ShutterMode": "Pre-Exposure", "COM3-Handshaking": "Off", "445 AO-SupportsTriggering": "0", "ROI": "0-0-1200-1200", "COM9-Verbose": "1", "PRIME-Color": "OFF", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "PRIME-ShutterOpenDelay": "0", "COM1-Parity": "None", "Slice": 8, "SlicePosition": 30, "COM9-DelayBetweenCharsMs": "0.0000", "555-Name": "DA Shutter", "PIEZO-LudlSingleAxisName": "B", "XLIGHT Emission Wheel-Label": "679/29 (A)", "640 Shutter-Name": "AnalogIO", "640 AO-Sequenceable": "1", "520 Shutter-Demo": "No", "528 Shutter-Demo": "No", "470-Name": "DA Shutter", "520-DA Device": "520 Shutter", "XYStage-Description": "Ludl XY stage driver adapter", "445 Shutter-MinVolts": "0.0000", "528 AO-TriggerSampleRate": "100000", "405 AO-MinVolts": "0.0000", "405 Shutter-TriggerSequenceLength": "1000", "Height": 1200, "555 AO-Block voltage": "false", "640 Shutter-Volts": "5.0000", "PixelType": "GRAY16", "445 Shutter-Block voltage": "false", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "520 Shutter-TriggerSequenceLength": "1000", "COM9-Name": "COM9", "Core-SLM": "", "555 AO-Volts": "0.0000", "555 Shutter-Block voltage": "false", "XYStage-Speed": "1800.0000", "520 AO-MaxVolts": "5.0000", "528 Shutter-SupportsTriggering": "0", "COM1-Description": "Serial port driver (boost:asio)", "PRIME-MetadataEnabled": "No", "520 Shutter-MinVolts": "0.0000", "470 Shutter-Sequenceable": "1", "PRIME-PreampDelay": "0", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-MinVolts": "0.0000", "ChannelIndex": 2, "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Core-AutoShutter": "1", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "Exposure-ms": 320, "PRIME-PP 2 ENABLED": "Yes", "640 Shutter-TriggerSampleRate": "100000", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "470 Shutter-IOChannel": "Dev1/ao10", "405 AO-Demo": "No", "PixelSizeUm": 1.1, "470 AO-Demo": "No", "470 Shutter-MaxVolts": "5.0000", "Vincent-D3-Name": "Vincent-D3", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FileName": "img_000000000_Il-A_008.tif", "LudlController-Reset": "Operate", "PRIME-Exposure": "320.00", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "445-Name": "DA Shutter", "555 Shutter-Name": "AnalogIO", "520 Shutter-TriggerSampleRate": "100000", "445 Shutter-IOChannel": "Dev1/ao9", "PRIME-TransposeCorrection": "0", "XYStage-StepSize": "0.1000", "445-Description": "DA device that is used as a shutter", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 AO-IOChannel": "Dev1/ao7", "520 Shutter-Name": "AnalogIO", "COM3-DataBits": "8", "640 AO-Volts": "4.5000", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "555 Shutter-IOChannel": "Dev1/ao13", "WaitInterval": null, "520 Shutter-Sequenceable": "1", "YPositionUm": 20690.300000000003, "445-State": "0", "445-DA Device": "445 Shutter", "PRIME-CircularBufferAutoSize": "ON", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "555 AO-TriggerSampleRate": "100000", "445 Shutter-Volts": "0.0000", "XLIGHT Hub-Port": "COM3", "Core-AutoFocus": "", "COM4-Handshaking": "Off", "528 Shutter-MinVolts": "0.0000", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "445 Shutter-Name": "AnalogIO", "528 Shutter-Sequenceable": "1", "XYStage-TransposeMirrorX": "0", "XYStage-TransposeMirrorY": "0", "PRIME-ReadoutRate": "100MHz 16bit", "XYStage-Name": "Stage", "640 AO-SupportsTriggering": "0", "640-Name": "DA Shutter", "640 Shutter-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "640 AO-Block voltage": "false", "445 Shutter-Sequenceable": "1", "470 AO-MaxVolts": "5.0000", "COM4-BaudRate": "9600", "470-Description": "DA device that is used as a shutter", "520 Shutter-Description": "NI DAC", "PRIME-CCDTemperature": "-14.9600", "Time": "2019-12-17 21:58:56 -0500", "640 AO-TriggerSequenceLength": "1000", "470 AO-Name": "AnalogIO", "XLIGHT Disk slider-Label": "Disk Pos 40um", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "Binning": "1x1", "TE2000-ModelType": "1", "COM3-AnswerTimeout": "500.0000", "555 Shutter-SupportsTriggering": "0", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "TE2000-Port": "COM4", "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Description": "NI DAC", "COM4-AnswerTimeout": "500.0000", "COM1-DelayBetweenCharsMs": "0.0000", "FilterBlock-Label": "Confocal", "445 AO-Name": "AnalogIO", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-Timing-ReadoutTimeNs": "24672000.0000", "528 AO-Demo": "No", "520 AO-Sequenceable": "1", "555-Description": "DA device that is used as a shutter", "405-DA Device": "405 Shutter", "405 AO-TriggerSequenceLength": "1000", "640 AO-MinVolts": "0.0000", "640 Shutter-Block voltage": "false", "FrameIndex": 0, "PRIME-Port": "CMOS", "555 AO-MinVolts": "0.0000", "COM3-Description": "Serial port driver (boost:asio)", "528 AO-Description": "NI DAC", "555 AO-IOChannel": "Dev1/ao6", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "555 AO-TriggerInputLine": "/Dev1/PFI0", "PRIME-ActualInterval-ms": "374.3700", "LudlController-Port": "COM9", "445 Shutter-Demo": "No", "PRIME-Trigger-Expose Out-Mux": "1", "SliceIndex": 8, "PRIME-CCDTemperatureSetPoint": "-15.0000", "405 Shutter-MinVolts": "0.0000", "PRIME-ExposeOutMode": "First Row", "AxisPositions": null, "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Volts": "0.0000", "528-Name": "DA Shutter", "555-State": "0", "XLIGHT Dichroic-Label": "Di03-R635-t1", "445 AO-Sequenceable": "1", "405-Name": "DA Shutter", "405 AO-IOChannel": "Dev1/ao0", "Core-Camera": "PRIME", "Focus-Name": "Focus", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "405 Shutter-IOChannel": "Dev1/ao8", "640 AO-Demo": "No", "640 Shutter-SupportsTriggering": "0", "445 AO-Demo": "No", "555-DA Device": "555 Shutter", "470 Shutter-Description": "NI DAC", "528 AO-Volts": "0.0000", "COM1-DataBits": "8", "Vincent-D3-Address": "x", "520 AO-SupportsTriggering": "0", "405-Description": "DA device that is used as a shutter", "640 Shutter-MinVolts": "0.0000", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "405 AO-Name": "AnalogIO", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "640 AO-TriggerInputLine": "/Dev1/PFI0", "405 AO-TriggerInputLine": "/Dev1/PFI0", "640-Description": "DA device that is used as a shutter", "445 Shutter-TriggerSampleRate": "100000", "470 Shutter-TriggerSequenceLength": "1000", "COM1-StopBits": "1", "520 Shutter-SupportsTriggering": "0", "405-State": "0", "Core-Initialize": "1", "XLIGHT Emission Wheel-State": "7", "520 AO-Description": "NI DAC", "528 AO-TriggerInputLine": "/Dev1/PFI0", "445 Shutter-MaxVolts": "5.0000", "COM9-Handshaking": "Off", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "XLIGHT Spin motor-Label": "On (spinning)", "PRIME-PP 5 ENABLED": "No", "UUID": "1e1744c2-31f7-48f0-8001-0c9393927d38", "PRIME-TriggerMode": "Internal Trigger", "COM1-Handshaking": "Off", "PRIME-Timing-PostTriggerDelayNs": "49344000.0000", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "Focus-Position": "16479.8000", "528 Shutter-Block voltage": "false", "Channel": "Il-A", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "555 Shutter-Demo": "No", "405 Shutter-Demo": "No", "528 AO-Name": "AnalogIO", "520-State": "0", "Core-Galvo": "", "405 Shutter-TriggerSampleRate": "100000", "Focus-FocusStepSize_(nm)": "100", "PRIME-PixelType": "16bit", "COM4-DataBits": "8", "470 AO-IOChannel": "Dev1/ao2", "520 AO-MinVolts": "0.0000", "COM9-Parity": "None", "PRIME-CircularBufferEnabled": "ON", "PRIME-PP 6 Reset": "No", "640 AO-Description": "NI DAC", "Source": "PRIME", "445 AO-MinVolts": "0.0000", "PIEZO-Name": "Stage", "445 AO-Description": "NI DAC", "PRIME-CircularBufferFrameCount": "46", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "520 AO-Volts": "0.0000", "528 Shutter-TriggerSampleRate": "100000", "XPositionUm": 62321, "OpticalPath-State": "4", "PRIME-PP 3 THRESHOLD": "80", "PRIME-PVCAM Version": "3.7.1", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Gain": "1-HDR", "640 Shutter-TriggerSequenceLength": "1000", "528 AO-SupportsTriggering": "0", "445 AO-TriggerInputLine": "/Dev1/PFI0", "OpticalPath-Name": "OpticalPath", "PRIME-PP 4 ENABLED": "Yes", "PRIME-PVCAM Adapter Version": "1.3.29", "COM3-Name": "COM3", "405 AO-MaxVolts": "5.0000", "520 Shutter-Block voltage": "false", "PRIME-Timing-ClearingTimeNs": "49344000.0000", "Core-Focus": "PIEZO", "528 AO-Block voltage": "false", "TE2000-Description": "Nikon TE2000 hub", "528 AO-MinVolts": "0.0000", "COM4-Parity": "None", "Summary": { "Slices": 11, "ChContrastMin": [ 0, 0, 0, 0, 0, 0 ], "PixelAspect": 1, "SlicesFirst": true, "Interval_ms": 0, "Time": "2019-12-17 21:58:39 -0500", "Prefix": "seq_3", "InitialPositionList": [ { "Label": "Pos0", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.435], "Focus": [16480.2], "XYStage": [ 69822.40000000001, 20100.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos1", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.425], "Focus": [16480.3], "XYStage": [ 69004, 20101 ] }, "GridColumnIndex": 0 }, { "Label": "Pos2", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.44], "Focus": [16480.1], "XYStage": [ 63119.5, 20690.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos3", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.405], "Focus": [16480.2], "XYStage": [ 62321, 20690.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos4", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.415], "Focus": [16480], "XYStage": [ 56604.100000000006, 20688.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos5", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.42000000000002], "Focus": [16480.1], "XYStage": [ 56034.200000000004, 20688.800000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos6", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.455], "Focus": [16480], "XYStage": [ 50889.3, 20496.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos7", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.43], "Focus": [16480.2], "XYStage": [ 50101.600000000006, 20496.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos8", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.67000000000002], "Focus": [16479.9], "XYStage": [ 44818.600000000006, 20496.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos9", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.67000000000002], "Focus": [16480], "XYStage": [ 43897.9, 20436.800000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos10", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.675], "Focus": [16479.9], "XYStage": [ 39670.600000000006, 20664.9 ] }, "GridColumnIndex": 0 }, { "Label": "Pos11", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.69], "Focus": [16479.9], "XYStage": [ 38734, 20664.600000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos12", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.695], "Focus": [16480.1], "XYStage": [ 34037.200000000004, 20664.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos13", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.685], "Focus": [16480.1], "XYStage": [ 33152, 20664.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos14", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.69], "Focus": [16480.1], "XYStage": [ 28122.800000000003, 20664.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos15", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.675], "Focus": [16480], "XYStage": [ 27422.9, 20664.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos16", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.64000000000001], "Focus": [16480], "XYStage": [ 23005.2, 20664.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos17", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.69], "Focus": [16479.9], "XYStage": [ 21820.600000000002, 20409.600000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos18", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.41], "Focus": [16480.1], "XYStage": [ 73697.6, -13222.800000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos19", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.455], "Focus": [16480.1], "XYStage": [ 72765.8, -13223 ] }, "GridColumnIndex": 0 }, { "Label": "Pos20", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.4], "Focus": [16480.2], "XYStage": [ 67445.5, -12303.800000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos21", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.415], "Focus": [16480.2], "XYStage": [ 66613.3, -12303.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos22", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.415], "Focus": [16480.1], "XYStage": [ 61819.4, -11936.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos23", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.405], "Focus": [16480.2], "XYStage": [ 61325.700000000004, -11937.1 ] }, "GridColumnIndex": 0 }, { "Label": "Pos24", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.46], "Focus": [16480.2], "XYStage": [ 55377, -10974.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos25", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.445], "Focus": [16480.1], "XYStage": [ 54827, -11214.800000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos26", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.435], "Focus": [16480.2], "XYStage": [ 49937.100000000006, -10919.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos27", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.425], "Focus": [16480.1], "XYStage": [ 48764, -10919.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos28", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.42000000000002], "Focus": [16480.2], "XYStage": [ 43978.4, -10920.400000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos29", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.435], "Focus": [16480.2], "XYStage": [ 43106.3, -10920.400000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos30", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.405], "Focus": [16480.3], "XYStage": [ 38212.4, -10920.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos31", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.445], "Focus": [16480.2], "XYStage": [ 37254.9, -10920.400000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos32", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.44], "Focus": [16480.3], "XYStage": [ 32472.800000000003, -11294.400000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos33", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [166.95000000000002], "Focus": [16480.2], "XYStage": [ 31420.7, -11294.400000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos34", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [166.95000000000002], "Focus": [16480.2], "XYStage": [ 26881.5, -11640 ] }, "GridColumnIndex": 0 }, { "Label": "Pos35", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [166.98], "Focus": [16480.2], "XYStage": [ 25876.100000000002, -11641.1 ] }, "GridColumnIndex": 0 } ], "z-step_um": 10, "GridRow": 0, "BitDepth": 16, "Depth": 2, "Channels": 6, "Source": "Micro-Manager", "ChColors": [ -16711681, -256, -65281, -1, -1, -16776961 ], "MetadataVersion": 10, "KeepShutterOpenChannels": true, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536, 65536 ], "CustomIntervals_ms": [], "Height": 1200, "MicroManagerVersion": "1.4.23 20180219", "GridColumn": 0, "UUID": "8c8f9d20-b128-4577-9f91-89e70f22d319", "PixelType": "GRAY16", "Width": 1200, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\Yan\\20191217-YC113-S5S6", "Frames": 1, "Comment": "", "UserName": "Nikon", "PixelSize_um": 1.1, "ROI": [ 0, 0, 1200, 1200 ], "Date": "2019-12-17", "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC", "P-DAPI-high" ], "KeepShutterOpenSlices": true, "IJType": 1, "ComputerName": "ZadorConfocal2", "Positions": 36, "PositionIndex": 3, "CameraTimeout": 20000, "TimeFirst": false }, "640 AO-Name": "AnalogIO", "Vincent-D3-Command": "Close" }, "FrameKey-0-2-9": { "COM4-StopBits": "1", "528 AO-MaxVolts": "5.0000", "XLIGHT Excitation Wheel-State": "2", "470 Shutter-Name": "AnalogIO", "Core-Shutter": "640", "555 AO-Demo": "No", "XLIGHT Dichroic-State": "2", "XYStage-StepSize-Y": "0.1000", "ZPositionUm": 217.405, "XYStage-StepSize-X": "0.1000", "PRIME-FirmwareVersion": "20.28", "PRIME-ClearCycles": "2", "520-Name": "DA Shutter", "405 Shutter-Name": "AnalogIO", "PIEZO-StepSize": "0.0050", "PRIME-ClearMode": "Pre-Exposure", "470 AO-MinVolts": "0.0000", "405 AO-SupportsTriggering": "1", "CameraChannelIndex": 0, "XYStage-StartSpeed": "500.0000", "COM9-StopBits": "2", "405 AO-Description": "NI DAC", "COM4-DelayBetweenCharsMs": "0.0000", "445 AO-Block voltage": "false", "520 AO-TriggerInputLine": "/Dev1/PFI0", "555 AO-Description": "NI DAC", "520 AO-TriggerSampleRate": "100000", "COM4-Verbose": "1", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM4-Name": "COM4", "405 Shutter-Volts": "0.0000", "Vincent-D3-Port": "COM1", "PRIME-PP 4 MIN ADU AFFECTED": "200", "470 Shutter-MinVolts": "0.0000", "PRIME-PP 3 ENABLED": "Yes", "470 AO-Description": "NI DAC", "PRIME-Description": "PVCAM API device adapter", "520 Shutter-MaxVolts": "5.0000", "520 Shutter-Volts": "0.0000", "528 AO-Sequenceable": "1", "PRIME-Binning": "1x1", "555 Shutter-MaxVolts": "5.0000", "528 Shutter-TriggerSequenceLength": "1000", "405 Shutter-Block voltage": "false", "XLIGHT Spin motor-State": "1", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "Vincent-D3-Time to open (ms)": "35.0000", "Vincent-D3-Time to close (ms)": "35.0000", "445 AO-TriggerSampleRate": "100000", "640 Shutter-IOChannel": "Dev1/ao14", "555 AO-Sequenceable": "1", "520 AO-Name": "AnalogIO", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "470 AO-Block voltage": "false", "COM1-Verbose": "1", "COM9-AnswerTimeout": "10000.0000", "528 Shutter-Name": "AnalogIO", "PRIME-Trigger Timeout (secs)": "10", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "NextFrame": 0, "470 AO-TriggerSequenceLength": "1000", "PRIME-TransposeXY": "0", "445 AO-TriggerSequenceLength": "1000", "COM1-Name": "COM1", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "COM9-DataBits": "8", "COM1-AnswerTimeout": "500.0000", "Camera": "", "TE2000-Version": "V2.50-V2.50-V2.10", "528 Shutter-Volts": "0.0000", "XLIGHT Hub-Name": "XLIGHT Hub", "PIEZO-Description": "Ludl stage driver adapter", "PositionName": "Pos3", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSampleRate": "100000", "PRIME-FanSpeedSetpoint": "High", "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "405 AO-TriggerSampleRate": "100000", "470 Shutter-Block voltage": "false", "470-State": "0", "COM3-Parity": "None", "405 AO-Volts": "0.0000", "PRIME-TransposeMirrorY": "0", "PRIME-ChipName": "GS144BSI", "470 AO-Volts": "0.0000", "PRIME-TransposeMirrorX": "0", "445 Shutter-SupportsTriggering": "0", "470 Shutter-SupportsTriggering": "0", "XLIGHT Hub-Description": "XLIGHT hub", "PRIME-PreampOffLimit": "10000", "470 AO-Sequenceable": "1", "528 Shutter-MaxVolts": "5.0000", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "Core-XYStage": "XYStage", "470 AO-TriggerSampleRate": "100000", "PRIME-Timing-PreTriggerDelayNs": "24672000.0000", "528-DA Device": "528 Shutter", "Focus-Description": "Nikon TE2000 focus adapter", "FilterBlock-State": "5", "520 AO-Demo": "No", "COM3-DelayBetweenCharsMs": "0.0000", "470 Shutter-Volts": "0.0000", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "PRIME-PP 2 THRESHOLD": "125", "PRIME-PP 4 THRESHOLD": "75", "XYStage-Acceleration": "4.0000", "470 Shutter-Demo": "No", "PRIME-Name": "PMPCIECam00", "640-DA Device": "640 Shutter", "405 AO-Block voltage": "false", "ElapsedTime-ms": 149504, "PRIME-Offset": "100", "445 AO-IOChannel": "Dev1/ao1", "640 AO-MaxVolts": "5.0000", "PositionIndex": 3, "PRIME-PP 1 ENABLED": "Yes", "COM1-BaudRate": "9600", "640-State": "1", "COM3-Verbose": "1", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "LudlController-TransmissionDelay": "0", "PRIME-FullWellCapacity": "62000", "PRIME-SMARTStreamingEnabled": "No", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "640 Shutter-Description": "NI DAC", "528-Description": "DA device that is used as a shutter", "COM4-Description": "Serial port driver (boost:asio)", "405 Shutter-Description": "NI DAC", "TE2000-Name": "TE2000", "528 Shutter-Description": "NI DAC", "COM9-Description": "Serial port driver (boost:asio)", "LudlController-Name": "LudlController", "445 Shutter-TriggerSequenceLength": "1000", "PRIME-SerialNumber": "A18B203008", "445 AO-Volts": "0.0000", "PRIME-X-dimension": "1200", "PRIME-ShutterCloseDelay": "0", "XLIGHT Disk slider-State": "2", "Position": "Default", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 AO-IOChannel": "Dev1/ao5", "520 AO-IOChannel": "Dev1/ao4", "528 AO-TriggerSequenceLength": "1000", "405 Shutter-MaxVolts": "5.0000", "470-DA Device": "470 Shutter", "PRIME-FTCapable": "No", "Core-ChannelGroup": "Channels", "555 AO-SupportsTriggering": "0", "PRIME-Y-dimension": "1200", "Frame": 0, "555 Shutter-Sequenceable": "1", "PRIME-Timing-ExposureTimeNs": "9992000.0000", "Core-ImageProcessor": "", "528-State": "0", "520 AO-Block voltage": "false", "XLIGHT Excitation Wheel-Label": "QUAD", "640 Shutter-Demo": "No", "COM3-StopBits": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "520 Shutter-IOChannel": "Dev1/ao11", "Width": 1200, "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-SupportsTriggering": "0", "640 AO-TriggerSampleRate": "100000", "520-Description": "DA device that is used as a shutter", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "405 Shutter-Sequenceable": "1", "405 Shutter-SupportsTriggering": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-TriggerSampleRate": "100000", "640 Shutter-MaxVolts": "5.0000", "555 AO-MaxVolts": "5.0000", "520 AO-TriggerSequenceLength": "1000", "COM3-BaudRate": "9600", "COM9-BaudRate": "9600", "Vincent-D3-Channel #": "Ch. #1", "PRIME-PMode": "Normal", "OpticalPath-Label": "Left(100)", "BitDepth": 16, "555 AO-TriggerSequenceLength": "1000", "445 Shutter-Description": "NI DAC", "405 AO-Sequenceable": "1", "PRIME-AcquisitionMethod": "Callbacks", "555 AO-Name": "AnalogIO", "PRIME-ShutterMode": "Pre-Exposure", "COM3-Handshaking": "Off", "445 AO-SupportsTriggering": "0", "ROI": "0-0-1200-1200", "COM9-Verbose": "1", "PRIME-Color": "OFF", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "PRIME-ShutterOpenDelay": "0", "COM1-Parity": "None", "Slice": 9, "SlicePosition": 40, "COM9-DelayBetweenCharsMs": "0.0000", "555-Name": "DA Shutter", "PIEZO-LudlSingleAxisName": "B", "XLIGHT Emission Wheel-Label": "679/29 (A)", "640 Shutter-Name": "AnalogIO", "640 AO-Sequenceable": "1", "520 Shutter-Demo": "No", "528 Shutter-Demo": "No", "470-Name": "DA Shutter", "520-DA Device": "520 Shutter", "XYStage-Description": "Ludl XY stage driver adapter", "445 Shutter-MinVolts": "0.0000", "528 AO-TriggerSampleRate": "100000", "405 AO-MinVolts": "0.0000", "405 Shutter-TriggerSequenceLength": "1000", "Height": 1200, "555 AO-Block voltage": "false", "640 Shutter-Volts": "5.0000", "PixelType": "GRAY16", "445 Shutter-Block voltage": "false", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "520 Shutter-TriggerSequenceLength": "1000", "COM9-Name": "COM9", "Core-SLM": "", "555 AO-Volts": "0.0000", "555 Shutter-Block voltage": "false", "XYStage-Speed": "1800.0000", "520 AO-MaxVolts": "5.0000", "528 Shutter-SupportsTriggering": "0", "COM1-Description": "Serial port driver (boost:asio)", "PRIME-MetadataEnabled": "No", "520 Shutter-MinVolts": "0.0000", "470 Shutter-Sequenceable": "1", "PRIME-PreampDelay": "0", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-MinVolts": "0.0000", "ChannelIndex": 2, "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Core-AutoShutter": "1", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "Exposure-ms": 320, "PRIME-PP 2 ENABLED": "Yes", "640 Shutter-TriggerSampleRate": "100000", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "470 Shutter-IOChannel": "Dev1/ao10", "405 AO-Demo": "No", "PixelSizeUm": 1.1, "470 AO-Demo": "No", "470 Shutter-MaxVolts": "5.0000", "Vincent-D3-Name": "Vincent-D3", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FileName": "img_000000000_Il-A_009.tif", "LudlController-Reset": "Operate", "PRIME-Exposure": "320.00", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "445-Name": "DA Shutter", "555 Shutter-Name": "AnalogIO", "520 Shutter-TriggerSampleRate": "100000", "445 Shutter-IOChannel": "Dev1/ao9", "PRIME-TransposeCorrection": "0", "XYStage-StepSize": "0.1000", "445-Description": "DA device that is used as a shutter", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 AO-IOChannel": "Dev1/ao7", "520 Shutter-Name": "AnalogIO", "COM3-DataBits": "8", "640 AO-Volts": "4.5000", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "555 Shutter-IOChannel": "Dev1/ao13", "WaitInterval": null, "520 Shutter-Sequenceable": "1", "YPositionUm": 20690.300000000003, "445-State": "0", "445-DA Device": "445 Shutter", "PRIME-CircularBufferAutoSize": "ON", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "555 AO-TriggerSampleRate": "100000", "445 Shutter-Volts": "0.0000", "XLIGHT Hub-Port": "COM3", "Core-AutoFocus": "", "COM4-Handshaking": "Off", "528 Shutter-MinVolts": "0.0000", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "445 Shutter-Name": "AnalogIO", "528 Shutter-Sequenceable": "1", "XYStage-TransposeMirrorX": "0", "XYStage-TransposeMirrorY": "0", "PRIME-ReadoutRate": "100MHz 16bit", "XYStage-Name": "Stage", "640 AO-SupportsTriggering": "0", "640-Name": "DA Shutter", "640 Shutter-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "640 AO-Block voltage": "false", "445 Shutter-Sequenceable": "1", "470 AO-MaxVolts": "5.0000", "COM4-BaudRate": "9600", "470-Description": "DA device that is used as a shutter", "520 Shutter-Description": "NI DAC", "PRIME-CCDTemperature": "-14.9600", "Time": "2019-12-17 21:58:56 -0500", "640 AO-TriggerSequenceLength": "1000", "470 AO-Name": "AnalogIO", "XLIGHT Disk slider-Label": "Disk Pos 40um", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "Binning": "1x1", "TE2000-ModelType": "1", "COM3-AnswerTimeout": "500.0000", "555 Shutter-SupportsTriggering": "0", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "TE2000-Port": "COM4", "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Description": "NI DAC", "COM4-AnswerTimeout": "500.0000", "COM1-DelayBetweenCharsMs": "0.0000", "FilterBlock-Label": "Confocal", "445 AO-Name": "AnalogIO", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-Timing-ReadoutTimeNs": "24672000.0000", "528 AO-Demo": "No", "520 AO-Sequenceable": "1", "555-Description": "DA device that is used as a shutter", "405-DA Device": "405 Shutter", "405 AO-TriggerSequenceLength": "1000", "640 AO-MinVolts": "0.0000", "640 Shutter-Block voltage": "false", "FrameIndex": 0, "PRIME-Port": "CMOS", "555 AO-MinVolts": "0.0000", "COM3-Description": "Serial port driver (boost:asio)", "528 AO-Description": "NI DAC", "555 AO-IOChannel": "Dev1/ao6", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "555 AO-TriggerInputLine": "/Dev1/PFI0", "PRIME-ActualInterval-ms": "374.3700", "LudlController-Port": "COM9", "445 Shutter-Demo": "No", "PRIME-Trigger-Expose Out-Mux": "1", "SliceIndex": 9, "PRIME-CCDTemperatureSetPoint": "-15.0000", "405 Shutter-MinVolts": "0.0000", "PRIME-ExposeOutMode": "First Row", "AxisPositions": null, "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Volts": "0.0000", "528-Name": "DA Shutter", "555-State": "0", "XLIGHT Dichroic-Label": "Di03-R635-t1", "445 AO-Sequenceable": "1", "405-Name": "DA Shutter", "405 AO-IOChannel": "Dev1/ao0", "Core-Camera": "PRIME", "Focus-Name": "Focus", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "405 Shutter-IOChannel": "Dev1/ao8", "640 AO-Demo": "No", "640 Shutter-SupportsTriggering": "0", "445 AO-Demo": "No", "555-DA Device": "555 Shutter", "470 Shutter-Description": "NI DAC", "528 AO-Volts": "0.0000", "COM1-DataBits": "8", "Vincent-D3-Address": "x", "520 AO-SupportsTriggering": "0", "405-Description": "DA device that is used as a shutter", "640 Shutter-MinVolts": "0.0000", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "405 AO-Name": "AnalogIO", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "640 AO-TriggerInputLine": "/Dev1/PFI0", "405 AO-TriggerInputLine": "/Dev1/PFI0", "640-Description": "DA device that is used as a shutter", "445 Shutter-TriggerSampleRate": "100000", "470 Shutter-TriggerSequenceLength": "1000", "COM1-StopBits": "1", "520 Shutter-SupportsTriggering": "0", "405-State": "0", "Core-Initialize": "1", "XLIGHT Emission Wheel-State": "7", "520 AO-Description": "NI DAC", "528 AO-TriggerInputLine": "/Dev1/PFI0", "445 Shutter-MaxVolts": "5.0000", "COM9-Handshaking": "Off", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "XLIGHT Spin motor-Label": "On (spinning)", "PRIME-PP 5 ENABLED": "No", "UUID": "58f3c0d8-cc64-4458-8428-2a71e93126a2", "PRIME-TriggerMode": "Internal Trigger", "COM1-Handshaking": "Off", "PRIME-Timing-PostTriggerDelayNs": "49344000.0000", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "Focus-Position": "16479.8000", "528 Shutter-Block voltage": "false", "Channel": "Il-A", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "555 Shutter-Demo": "No", "405 Shutter-Demo": "No", "528 AO-Name": "AnalogIO", "520-State": "0", "Core-Galvo": "", "405 Shutter-TriggerSampleRate": "100000", "Focus-FocusStepSize_(nm)": "100", "PRIME-PixelType": "16bit", "COM4-DataBits": "8", "470 AO-IOChannel": "Dev1/ao2", "520 AO-MinVolts": "0.0000", "COM9-Parity": "None", "PRIME-CircularBufferEnabled": "ON", "PRIME-PP 6 Reset": "No", "640 AO-Description": "NI DAC", "Source": "PRIME", "445 AO-MinVolts": "0.0000", "PIEZO-Name": "Stage", "445 AO-Description": "NI DAC", "PRIME-CircularBufferFrameCount": "46", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "520 AO-Volts": "0.0000", "528 Shutter-TriggerSampleRate": "100000", "XPositionUm": 62321, "OpticalPath-State": "4", "PRIME-PP 3 THRESHOLD": "80", "PRIME-PVCAM Version": "3.7.1", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Gain": "1-HDR", "640 Shutter-TriggerSequenceLength": "1000", "528 AO-SupportsTriggering": "0", "445 AO-TriggerInputLine": "/Dev1/PFI0", "OpticalPath-Name": "OpticalPath", "PRIME-PP 4 ENABLED": "Yes", "PRIME-PVCAM Adapter Version": "1.3.29", "COM3-Name": "COM3", "405 AO-MaxVolts": "5.0000", "520 Shutter-Block voltage": "false", "PRIME-Timing-ClearingTimeNs": "49344000.0000", "Core-Focus": "PIEZO", "528 AO-Block voltage": "false", "TE2000-Description": "Nikon TE2000 hub", "528 AO-MinVolts": "0.0000", "COM4-Parity": "None", "Summary": { "Slices": 11, "ChContrastMin": [ 0, 0, 0, 0, 0, 0 ], "PixelAspect": 1, "SlicesFirst": true, "Interval_ms": 0, "Time": "2019-12-17 21:58:39 -0500", "Prefix": "seq_3", "InitialPositionList": [ { "Label": "Pos0", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.435], "Focus": [16480.2], "XYStage": [ 69822.40000000001, 20100.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos1", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.425], "Focus": [16480.3], "XYStage": [ 69004, 20101 ] }, "GridColumnIndex": 0 }, { "Label": "Pos2", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.44], "Focus": [16480.1], "XYStage": [ 63119.5, 20690.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos3", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.405], "Focus": [16480.2], "XYStage": [ 62321, 20690.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos4", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.415], "Focus": [16480], "XYStage": [ 56604.100000000006, 20688.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos5", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.42000000000002], "Focus": [16480.1], "XYStage": [ 56034.200000000004, 20688.800000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos6", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.455], "Focus": [16480], "XYStage": [ 50889.3, 20496.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos7", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.43], "Focus": [16480.2], "XYStage": [ 50101.600000000006, 20496.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos8", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.67000000000002], "Focus": [16479.9], "XYStage": [ 44818.600000000006, 20496.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos9", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.67000000000002], "Focus": [16480], "XYStage": [ 43897.9, 20436.800000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos10", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.675], "Focus": [16479.9], "XYStage": [ 39670.600000000006, 20664.9 ] }, "GridColumnIndex": 0 }, { "Label": "Pos11", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.69], "Focus": [16479.9], "XYStage": [ 38734, 20664.600000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos12", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.695], "Focus": [16480.1], "XYStage": [ 34037.200000000004, 20664.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos13", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.685], "Focus": [16480.1], "XYStage": [ 33152, 20664.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos14", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.69], "Focus": [16480.1], "XYStage": [ 28122.800000000003, 20664.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos15", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.675], "Focus": [16480], "XYStage": [ 27422.9, 20664.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos16", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.64000000000001], "Focus": [16480], "XYStage": [ 23005.2, 20664.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos17", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.69], "Focus": [16479.9], "XYStage": [ 21820.600000000002, 20409.600000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos18", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.41], "Focus": [16480.1], "XYStage": [ 73697.6, -13222.800000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos19", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.455], "Focus": [16480.1], "XYStage": [ 72765.8, -13223 ] }, "GridColumnIndex": 0 }, { "Label": "Pos20", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.4], "Focus": [16480.2], "XYStage": [ 67445.5, -12303.800000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos21", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.415], "Focus": [16480.2], "XYStage": [ 66613.3, -12303.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos22", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.415], "Focus": [16480.1], "XYStage": [ 61819.4, -11936.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos23", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.405], "Focus": [16480.2], "XYStage": [ 61325.700000000004, -11937.1 ] }, "GridColumnIndex": 0 }, { "Label": "Pos24", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.46], "Focus": [16480.2], "XYStage": [ 55377, -10974.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos25", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.445], "Focus": [16480.1], "XYStage": [ 54827, -11214.800000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos26", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.435], "Focus": [16480.2], "XYStage": [ 49937.100000000006, -10919.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos27", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.425], "Focus": [16480.1], "XYStage": [ 48764, -10919.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos28", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.42000000000002], "Focus": [16480.2], "XYStage": [ 43978.4, -10920.400000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos29", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.435], "Focus": [16480.2], "XYStage": [ 43106.3, -10920.400000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos30", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.405], "Focus": [16480.3], "XYStage": [ 38212.4, -10920.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos31", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.445], "Focus": [16480.2], "XYStage": [ 37254.9, -10920.400000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos32", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.44], "Focus": [16480.3], "XYStage": [ 32472.800000000003, -11294.400000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos33", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [166.95000000000002], "Focus": [16480.2], "XYStage": [ 31420.7, -11294.400000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos34", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [166.95000000000002], "Focus": [16480.2], "XYStage": [ 26881.5, -11640 ] }, "GridColumnIndex": 0 }, { "Label": "Pos35", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [166.98], "Focus": [16480.2], "XYStage": [ 25876.100000000002, -11641.1 ] }, "GridColumnIndex": 0 } ], "z-step_um": 10, "GridRow": 0, "BitDepth": 16, "Depth": 2, "Channels": 6, "Source": "Micro-Manager", "ChColors": [ -16711681, -256, -65281, -1, -1, -16776961 ], "MetadataVersion": 10, "KeepShutterOpenChannels": true, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536, 65536 ], "CustomIntervals_ms": [], "Height": 1200, "MicroManagerVersion": "1.4.23 20180219", "GridColumn": 0, "UUID": "8c8f9d20-b128-4577-9f91-89e70f22d319", "PixelType": "GRAY16", "Width": 1200, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\Yan\\20191217-YC113-S5S6", "Frames": 1, "Comment": "", "UserName": "Nikon", "PixelSize_um": 1.1, "ROI": [ 0, 0, 1200, 1200 ], "Date": "2019-12-17", "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC", "P-DAPI-high" ], "KeepShutterOpenSlices": true, "IJType": 1, "ComputerName": "ZadorConfocal2", "Positions": 36, "PositionIndex": 3, "CameraTimeout": 20000, "TimeFirst": false }, "640 AO-Name": "AnalogIO", "Vincent-D3-Command": "Close" }, "FrameKey-0-2-10": { "COM4-StopBits": "1", "528 AO-MaxVolts": "5.0000", "XLIGHT Excitation Wheel-State": "2", "470 Shutter-Name": "AnalogIO", "Core-Shutter": "640", "555 AO-Demo": "No", "XLIGHT Dichroic-State": "2", "XYStage-StepSize-Y": "0.1000", "ZPositionUm": 227.405, "XYStage-StepSize-X": "0.1000", "PRIME-FirmwareVersion": "20.28", "PRIME-ClearCycles": "2", "520-Name": "DA Shutter", "405 Shutter-Name": "AnalogIO", "PIEZO-StepSize": "0.0050", "PRIME-ClearMode": "Pre-Exposure", "470 AO-MinVolts": "0.0000", "405 AO-SupportsTriggering": "1", "CameraChannelIndex": 0, "XYStage-StartSpeed": "500.0000", "COM9-StopBits": "2", "405 AO-Description": "NI DAC", "COM4-DelayBetweenCharsMs": "0.0000", "445 AO-Block voltage": "false", "520 AO-TriggerInputLine": "/Dev1/PFI0", "555 AO-Description": "NI DAC", "520 AO-TriggerSampleRate": "100000", "COM4-Verbose": "1", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM4-Name": "COM4", "405 Shutter-Volts": "0.0000", "Vincent-D3-Port": "COM1", "PRIME-PP 4 MIN ADU AFFECTED": "200", "470 Shutter-MinVolts": "0.0000", "PRIME-PP 3 ENABLED": "Yes", "470 AO-Description": "NI DAC", "PRIME-Description": "PVCAM API device adapter", "520 Shutter-MaxVolts": "5.0000", "520 Shutter-Volts": "0.0000", "528 AO-Sequenceable": "1", "PRIME-Binning": "1x1", "555 Shutter-MaxVolts": "5.0000", "528 Shutter-TriggerSequenceLength": "1000", "405 Shutter-Block voltage": "false", "XLIGHT Spin motor-State": "1", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "Vincent-D3-Time to open (ms)": "35.0000", "Vincent-D3-Time to close (ms)": "35.0000", "445 AO-TriggerSampleRate": "100000", "640 Shutter-IOChannel": "Dev1/ao14", "555 AO-Sequenceable": "1", "520 AO-Name": "AnalogIO", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "470 AO-Block voltage": "false", "COM1-Verbose": "1", "COM9-AnswerTimeout": "10000.0000", "528 Shutter-Name": "AnalogIO", "PRIME-Trigger Timeout (secs)": "10", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "NextFrame": 0, "470 AO-TriggerSequenceLength": "1000", "PRIME-TransposeXY": "0", "445 AO-TriggerSequenceLength": "1000", "COM1-Name": "COM1", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "COM9-DataBits": "8", "COM1-AnswerTimeout": "500.0000", "Camera": "", "TE2000-Version": "V2.50-V2.50-V2.10", "528 Shutter-Volts": "0.0000", "XLIGHT Hub-Name": "XLIGHT Hub", "PIEZO-Description": "Ludl stage driver adapter", "PositionName": "Pos3", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSampleRate": "100000", "PRIME-FanSpeedSetpoint": "High", "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "405 AO-TriggerSampleRate": "100000", "470 Shutter-Block voltage": "false", "470-State": "0", "COM3-Parity": "None", "405 AO-Volts": "0.0000", "PRIME-TransposeMirrorY": "0", "PRIME-ChipName": "GS144BSI", "470 AO-Volts": "0.0000", "PRIME-TransposeMirrorX": "0", "445 Shutter-SupportsTriggering": "0", "470 Shutter-SupportsTriggering": "0", "XLIGHT Hub-Description": "XLIGHT hub", "PRIME-PreampOffLimit": "10000", "470 AO-Sequenceable": "1", "528 Shutter-MaxVolts": "5.0000", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "Core-XYStage": "XYStage", "470 AO-TriggerSampleRate": "100000", "PRIME-Timing-PreTriggerDelayNs": "24672000.0000", "528-DA Device": "528 Shutter", "Focus-Description": "Nikon TE2000 focus adapter", "FilterBlock-State": "5", "520 AO-Demo": "No", "COM3-DelayBetweenCharsMs": "0.0000", "470 Shutter-Volts": "0.0000", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "PRIME-PP 2 THRESHOLD": "125", "PRIME-PP 4 THRESHOLD": "75", "XYStage-Acceleration": "4.0000", "470 Shutter-Demo": "No", "PRIME-Name": "PMPCIECam00", "640-DA Device": "640 Shutter", "405 AO-Block voltage": "false", "ElapsedTime-ms": 150063, "PRIME-Offset": "100", "445 AO-IOChannel": "Dev1/ao1", "640 AO-MaxVolts": "5.0000", "PositionIndex": 3, "PRIME-PP 1 ENABLED": "Yes", "COM1-BaudRate": "9600", "640-State": "1", "COM3-Verbose": "1", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "LudlController-TransmissionDelay": "0", "PRIME-FullWellCapacity": "62000", "PRIME-SMARTStreamingEnabled": "No", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "640 Shutter-Description": "NI DAC", "528-Description": "DA device that is used as a shutter", "COM4-Description": "Serial port driver (boost:asio)", "405 Shutter-Description": "NI DAC", "TE2000-Name": "TE2000", "528 Shutter-Description": "NI DAC", "COM9-Description": "Serial port driver (boost:asio)", "LudlController-Name": "LudlController", "445 Shutter-TriggerSequenceLength": "1000", "PRIME-SerialNumber": "A18B203008", "445 AO-Volts": "0.0000", "PRIME-X-dimension": "1200", "PRIME-ShutterCloseDelay": "0", "XLIGHT Disk slider-State": "2", "Position": "Default", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 AO-IOChannel": "Dev1/ao5", "520 AO-IOChannel": "Dev1/ao4", "528 AO-TriggerSequenceLength": "1000", "405 Shutter-MaxVolts": "5.0000", "470-DA Device": "470 Shutter", "PRIME-FTCapable": "No", "Core-ChannelGroup": "Channels", "555 AO-SupportsTriggering": "0", "PRIME-Y-dimension": "1200", "Frame": 0, "555 Shutter-Sequenceable": "1", "PRIME-Timing-ExposureTimeNs": "9992000.0000", "Core-ImageProcessor": "", "528-State": "0", "520 AO-Block voltage": "false", "XLIGHT Excitation Wheel-Label": "QUAD", "640 Shutter-Demo": "No", "COM3-StopBits": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "520 Shutter-IOChannel": "Dev1/ao11", "Width": 1200, "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-SupportsTriggering": "0", "640 AO-TriggerSampleRate": "100000", "520-Description": "DA device that is used as a shutter", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "405 Shutter-Sequenceable": "1", "405 Shutter-SupportsTriggering": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-TriggerSampleRate": "100000", "640 Shutter-MaxVolts": "5.0000", "555 AO-MaxVolts": "5.0000", "520 AO-TriggerSequenceLength": "1000", "COM3-BaudRate": "9600", "COM9-BaudRate": "9600", "Vincent-D3-Channel #": "Ch. #1", "PRIME-PMode": "Normal", "OpticalPath-Label": "Left(100)", "BitDepth": 16, "555 AO-TriggerSequenceLength": "1000", "445 Shutter-Description": "NI DAC", "405 AO-Sequenceable": "1", "PRIME-AcquisitionMethod": "Callbacks", "555 AO-Name": "AnalogIO", "PRIME-ShutterMode": "Pre-Exposure", "COM3-Handshaking": "Off", "445 AO-SupportsTriggering": "0", "ROI": "0-0-1200-1200", "COM9-Verbose": "1", "PRIME-Color": "OFF", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "PRIME-ShutterOpenDelay": "0", "COM1-Parity": "None", "Slice": 10, "SlicePosition": 50, "COM9-DelayBetweenCharsMs": "0.0000", "555-Name": "DA Shutter", "PIEZO-LudlSingleAxisName": "B", "XLIGHT Emission Wheel-Label": "679/29 (A)", "640 Shutter-Name": "AnalogIO", "640 AO-Sequenceable": "1", "520 Shutter-Demo": "No", "528 Shutter-Demo": "No", "470-Name": "DA Shutter", "520-DA Device": "520 Shutter", "XYStage-Description": "Ludl XY stage driver adapter", "445 Shutter-MinVolts": "0.0000", "528 AO-TriggerSampleRate": "100000", "405 AO-MinVolts": "0.0000", "405 Shutter-TriggerSequenceLength": "1000", "Height": 1200, "555 AO-Block voltage": "false", "640 Shutter-Volts": "5.0000", "PixelType": "GRAY16", "445 Shutter-Block voltage": "false", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "520 Shutter-TriggerSequenceLength": "1000", "COM9-Name": "COM9", "Core-SLM": "", "555 AO-Volts": "0.0000", "555 Shutter-Block voltage": "false", "XYStage-Speed": "1800.0000", "520 AO-MaxVolts": "5.0000", "528 Shutter-SupportsTriggering": "0", "COM1-Description": "Serial port driver (boost:asio)", "PRIME-MetadataEnabled": "No", "520 Shutter-MinVolts": "0.0000", "470 Shutter-Sequenceable": "1", "PRIME-PreampDelay": "0", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-MinVolts": "0.0000", "ChannelIndex": 2, "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Core-AutoShutter": "1", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "Exposure-ms": 320, "PRIME-PP 2 ENABLED": "Yes", "640 Shutter-TriggerSampleRate": "100000", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "470 Shutter-IOChannel": "Dev1/ao10", "405 AO-Demo": "No", "PixelSizeUm": 1.1, "470 AO-Demo": "No", "470 Shutter-MaxVolts": "5.0000", "Vincent-D3-Name": "Vincent-D3", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FileName": "img_000000000_Il-A_010.tif", "LudlController-Reset": "Operate", "PRIME-Exposure": "320.00", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "445-Name": "DA Shutter", "555 Shutter-Name": "AnalogIO", "520 Shutter-TriggerSampleRate": "100000", "445 Shutter-IOChannel": "Dev1/ao9", "PRIME-TransposeCorrection": "0", "XYStage-StepSize": "0.1000", "445-Description": "DA device that is used as a shutter", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 AO-IOChannel": "Dev1/ao7", "520 Shutter-Name": "AnalogIO", "COM3-DataBits": "8", "640 AO-Volts": "4.5000", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "555 Shutter-IOChannel": "Dev1/ao13", "WaitInterval": null, "520 Shutter-Sequenceable": "1", "YPositionUm": 20690.300000000003, "445-State": "0", "445-DA Device": "445 Shutter", "PRIME-CircularBufferAutoSize": "ON", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "555 AO-TriggerSampleRate": "100000", "445 Shutter-Volts": "0.0000", "XLIGHT Hub-Port": "COM3", "Core-AutoFocus": "", "COM4-Handshaking": "Off", "528 Shutter-MinVolts": "0.0000", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "445 Shutter-Name": "AnalogIO", "528 Shutter-Sequenceable": "1", "XYStage-TransposeMirrorX": "0", "XYStage-TransposeMirrorY": "0", "PRIME-ReadoutRate": "100MHz 16bit", "XYStage-Name": "Stage", "640 AO-SupportsTriggering": "0", "640-Name": "DA Shutter", "640 Shutter-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "640 AO-Block voltage": "false", "445 Shutter-Sequenceable": "1", "470 AO-MaxVolts": "5.0000", "COM4-BaudRate": "9600", "470-Description": "DA device that is used as a shutter", "520 Shutter-Description": "NI DAC", "PRIME-CCDTemperature": "-14.9600", "Time": "2019-12-17 21:58:57 -0500", "640 AO-TriggerSequenceLength": "1000", "470 AO-Name": "AnalogIO", "XLIGHT Disk slider-Label": "Disk Pos 40um", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "Binning": "1x1", "TE2000-ModelType": "1", "COM3-AnswerTimeout": "500.0000", "555 Shutter-SupportsTriggering": "0", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "TE2000-Port": "COM4", "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Description": "NI DAC", "COM4-AnswerTimeout": "500.0000", "COM1-DelayBetweenCharsMs": "0.0000", "FilterBlock-Label": "Confocal", "445 AO-Name": "AnalogIO", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-Timing-ReadoutTimeNs": "24672000.0000", "528 AO-Demo": "No", "520 AO-Sequenceable": "1", "555-Description": "DA device that is used as a shutter", "405-DA Device": "405 Shutter", "405 AO-TriggerSequenceLength": "1000", "640 AO-MinVolts": "0.0000", "640 Shutter-Block voltage": "false", "FrameIndex": 0, "PRIME-Port": "CMOS", "555 AO-MinVolts": "0.0000", "COM3-Description": "Serial port driver (boost:asio)", "528 AO-Description": "NI DAC", "555 AO-IOChannel": "Dev1/ao6", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "555 AO-TriggerInputLine": "/Dev1/PFI0", "PRIME-ActualInterval-ms": "374.3700", "LudlController-Port": "COM9", "445 Shutter-Demo": "No", "PRIME-Trigger-Expose Out-Mux": "1", "SliceIndex": 10, "PRIME-CCDTemperatureSetPoint": "-15.0000", "405 Shutter-MinVolts": "0.0000", "PRIME-ExposeOutMode": "First Row", "AxisPositions": null, "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Volts": "0.0000", "528-Name": "DA Shutter", "555-State": "0", "XLIGHT Dichroic-Label": "Di03-R635-t1", "445 AO-Sequenceable": "1", "405-Name": "DA Shutter", "405 AO-IOChannel": "Dev1/ao0", "Core-Camera": "PRIME", "Focus-Name": "Focus", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "405 Shutter-IOChannel": "Dev1/ao8", "640 AO-Demo": "No", "640 Shutter-SupportsTriggering": "0", "445 AO-Demo": "No", "555-DA Device": "555 Shutter", "470 Shutter-Description": "NI DAC", "528 AO-Volts": "0.0000", "COM1-DataBits": "8", "Vincent-D3-Address": "x", "520 AO-SupportsTriggering": "0", "405-Description": "DA device that is used as a shutter", "640 Shutter-MinVolts": "0.0000", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "405 AO-Name": "AnalogIO", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "640 AO-TriggerInputLine": "/Dev1/PFI0", "405 AO-TriggerInputLine": "/Dev1/PFI0", "640-Description": "DA device that is used as a shutter", "445 Shutter-TriggerSampleRate": "100000", "470 Shutter-TriggerSequenceLength": "1000", "COM1-StopBits": "1", "520 Shutter-SupportsTriggering": "0", "405-State": "0", "Core-Initialize": "1", "XLIGHT Emission Wheel-State": "7", "520 AO-Description": "NI DAC", "528 AO-TriggerInputLine": "/Dev1/PFI0", "445 Shutter-MaxVolts": "5.0000", "COM9-Handshaking": "Off", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "XLIGHT Spin motor-Label": "On (spinning)", "PRIME-PP 5 ENABLED": "No", "UUID": "d53df39c-fd0a-4dda-8855-4606466f55f3", "PRIME-TriggerMode": "Internal Trigger", "COM1-Handshaking": "Off", "PRIME-Timing-PostTriggerDelayNs": "49344000.0000", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "Focus-Position": "16479.8000", "528 Shutter-Block voltage": "false", "Channel": "Il-A", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "555 Shutter-Demo": "No", "405 Shutter-Demo": "No", "528 AO-Name": "AnalogIO", "520-State": "0", "Core-Galvo": "", "405 Shutter-TriggerSampleRate": "100000", "Focus-FocusStepSize_(nm)": "100", "PRIME-PixelType": "16bit", "COM4-DataBits": "8", "470 AO-IOChannel": "Dev1/ao2", "520 AO-MinVolts": "0.0000", "COM9-Parity": "None", "PRIME-CircularBufferEnabled": "ON", "PRIME-PP 6 Reset": "No", "640 AO-Description": "NI DAC", "Source": "PRIME", "445 AO-MinVolts": "0.0000", "PIEZO-Name": "Stage", "445 AO-Description": "NI DAC", "PRIME-CircularBufferFrameCount": "46", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "520 AO-Volts": "0.0000", "528 Shutter-TriggerSampleRate": "100000", "XPositionUm": 62321, "OpticalPath-State": "4", "PRIME-PP 3 THRESHOLD": "80", "PRIME-PVCAM Version": "3.7.1", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Gain": "1-HDR", "640 Shutter-TriggerSequenceLength": "1000", "528 AO-SupportsTriggering": "0", "445 AO-TriggerInputLine": "/Dev1/PFI0", "OpticalPath-Name": "OpticalPath", "PRIME-PP 4 ENABLED": "Yes", "PRIME-PVCAM Adapter Version": "1.3.29", "COM3-Name": "COM3", "405 AO-MaxVolts": "5.0000", "520 Shutter-Block voltage": "false", "PRIME-Timing-ClearingTimeNs": "49344000.0000", "Core-Focus": "PIEZO", "528 AO-Block voltage": "false", "TE2000-Description": "Nikon TE2000 hub", "528 AO-MinVolts": "0.0000", "COM4-Parity": "None", "Summary": { "Slices": 11, "ChContrastMin": [ 0, 0, 0, 0, 0, 0 ], "PixelAspect": 1, "SlicesFirst": true, "Interval_ms": 0, "Time": "2019-12-17 21:58:39 -0500", "Prefix": "seq_3", "InitialPositionList": [ { "Label": "Pos0", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.435], "Focus": [16480.2], "XYStage": [ 69822.40000000001, 20100.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos1", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.425], "Focus": [16480.3], "XYStage": [ 69004, 20101 ] }, "GridColumnIndex": 0 }, { "Label": "Pos2", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.44], "Focus": [16480.1], "XYStage": [ 63119.5, 20690.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos3", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.405], "Focus": [16480.2], "XYStage": [ 62321, 20690.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos4", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.415], "Focus": [16480], "XYStage": [ 56604.100000000006, 20688.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos5", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.42000000000002], "Focus": [16480.1], "XYStage": [ 56034.200000000004, 20688.800000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos6", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.455], "Focus": [16480], "XYStage": [ 50889.3, 20496.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos7", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.43], "Focus": [16480.2], "XYStage": [ 50101.600000000006, 20496.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos8", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.67000000000002], "Focus": [16479.9], "XYStage": [ 44818.600000000006, 20496.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos9", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.67000000000002], "Focus": [16480], "XYStage": [ 43897.9, 20436.800000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos10", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.675], "Focus": [16479.9], "XYStage": [ 39670.600000000006, 20664.9 ] }, "GridColumnIndex": 0 }, { "Label": "Pos11", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.69], "Focus": [16479.9], "XYStage": [ 38734, 20664.600000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos12", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.695], "Focus": [16480.1], "XYStage": [ 34037.200000000004, 20664.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos13", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.685], "Focus": [16480.1], "XYStage": [ 33152, 20664.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos14", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.69], "Focus": [16480.1], "XYStage": [ 28122.800000000003, 20664.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos15", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.675], "Focus": [16480], "XYStage": [ 27422.9, 20664.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos16", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.64000000000001], "Focus": [16480], "XYStage": [ 23005.2, 20664.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos17", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.69], "Focus": [16479.9], "XYStage": [ 21820.600000000002, 20409.600000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos18", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.41], "Focus": [16480.1], "XYStage": [ 73697.6, -13222.800000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos19", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.455], "Focus": [16480.1], "XYStage": [ 72765.8, -13223 ] }, "GridColumnIndex": 0 }, { "Label": "Pos20", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.4], "Focus": [16480.2], "XYStage": [ 67445.5, -12303.800000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos21", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.415], "Focus": [16480.2], "XYStage": [ 66613.3, -12303.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos22", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.415], "Focus": [16480.1], "XYStage": [ 61819.4, -11936.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos23", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.405], "Focus": [16480.2], "XYStage": [ 61325.700000000004, -11937.1 ] }, "GridColumnIndex": 0 }, { "Label": "Pos24", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.46], "Focus": [16480.2], "XYStage": [ 55377, -10974.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos25", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.445], "Focus": [16480.1], "XYStage": [ 54827, -11214.800000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos26", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.435], "Focus": [16480.2], "XYStage": [ 49937.100000000006, -10919.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos27", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.425], "Focus": [16480.1], "XYStage": [ 48764, -10919.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos28", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.42000000000002], "Focus": [16480.2], "XYStage": [ 43978.4, -10920.400000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos29", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.435], "Focus": [16480.2], "XYStage": [ 43106.3, -10920.400000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos30", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.405], "Focus": [16480.3], "XYStage": [ 38212.4, -10920.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos31", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.445], "Focus": [16480.2], "XYStage": [ 37254.9, -10920.400000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos32", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.44], "Focus": [16480.3], "XYStage": [ 32472.800000000003, -11294.400000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos33", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [166.95000000000002], "Focus": [16480.2], "XYStage": [ 31420.7, -11294.400000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos34", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [166.95000000000002], "Focus": [16480.2], "XYStage": [ 26881.5, -11640 ] }, "GridColumnIndex": 0 }, { "Label": "Pos35", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [166.98], "Focus": [16480.2], "XYStage": [ 25876.100000000002, -11641.1 ] }, "GridColumnIndex": 0 } ], "z-step_um": 10, "GridRow": 0, "BitDepth": 16, "Depth": 2, "Channels": 6, "Source": "Micro-Manager", "ChColors": [ -16711681, -256, -65281, -1, -1, -16776961 ], "MetadataVersion": 10, "KeepShutterOpenChannels": true, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536, 65536 ], "CustomIntervals_ms": [], "Height": 1200, "MicroManagerVersion": "1.4.23 20180219", "GridColumn": 0, "UUID": "8c8f9d20-b128-4577-9f91-89e70f22d319", "PixelType": "GRAY16", "Width": 1200, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\Yan\\20191217-YC113-S5S6", "Frames": 1, "Comment": "", "UserName": "Nikon", "PixelSize_um": 1.1, "ROI": [ 0, 0, 1200, 1200 ], "Date": "2019-12-17", "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC", "P-DAPI-high" ], "KeepShutterOpenSlices": true, "IJType": 1, "ComputerName": "ZadorConfocal2", "Positions": 36, "PositionIndex": 3, "CameraTimeout": 20000, "TimeFirst": false }, "640 AO-Name": "AnalogIO", "Vincent-D3-Command": "Close" }, "FrameKey-0-3-0": { "COM4-StopBits": "1", "528 AO-MaxVolts": "5.0000", "XLIGHT Excitation Wheel-State": "2", "470 Shutter-Name": "AnalogIO", "Core-Shutter": "640", "555 AO-Demo": "No", "XLIGHT Dichroic-State": "2", "XYStage-StepSize-Y": "0.1000", "ZPositionUm": 127.405, "XYStage-StepSize-X": "0.1000", "PRIME-FirmwareVersion": "20.28", "PRIME-ClearCycles": "2", "520-Name": "DA Shutter", "405 Shutter-Name": "AnalogIO", "PIEZO-StepSize": "0.0050", "PRIME-ClearMode": "Pre-Exposure", "470 AO-MinVolts": "0.0000", "405 AO-SupportsTriggering": "1", "CameraChannelIndex": 0, "XYStage-StartSpeed": "500.0000", "COM9-StopBits": "2", "405 AO-Description": "NI DAC", "COM4-DelayBetweenCharsMs": "0.0000", "445 AO-Block voltage": "false", "520 AO-TriggerInputLine": "/Dev1/PFI0", "555 AO-Description": "NI DAC", "520 AO-TriggerSampleRate": "100000", "COM4-Verbose": "1", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM4-Name": "COM4", "405 Shutter-Volts": "0.0000", "Vincent-D3-Port": "COM1", "PRIME-PP 4 MIN ADU AFFECTED": "200", "470 Shutter-MinVolts": "0.0000", "PRIME-PP 3 ENABLED": "Yes", "470 AO-Description": "NI DAC", "PRIME-Description": "PVCAM API device adapter", "520 Shutter-MaxVolts": "5.0000", "520 Shutter-Volts": "0.0000", "528 AO-Sequenceable": "1", "PRIME-Binning": "1x1", "555 Shutter-MaxVolts": "5.0000", "528 Shutter-TriggerSequenceLength": "1000", "405 Shutter-Block voltage": "false", "XLIGHT Spin motor-State": "1", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "Vincent-D3-Time to open (ms)": "35.0000", "Vincent-D3-Time to close (ms)": "35.0000", "445 AO-TriggerSampleRate": "100000", "640 Shutter-IOChannel": "Dev1/ao14", "555 AO-Sequenceable": "1", "520 AO-Name": "AnalogIO", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "470 AO-Block voltage": "false", "COM1-Verbose": "1", "COM9-AnswerTimeout": "10000.0000", "528 Shutter-Name": "AnalogIO", "PRIME-Trigger Timeout (secs)": "10", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "NextFrame": 0, "470 AO-TriggerSequenceLength": "1000", "PRIME-TransposeXY": "0", "445 AO-TriggerSequenceLength": "1000", "COM1-Name": "COM1", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "COM9-DataBits": "8", "COM1-AnswerTimeout": "500.0000", "Camera": "", "TE2000-Version": "V2.50-V2.50-V2.10", "528 Shutter-Volts": "0.0000", "XLIGHT Hub-Name": "XLIGHT Hub", "PIEZO-Description": "Ludl stage driver adapter", "PositionName": "Pos3", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSampleRate": "100000", "PRIME-FanSpeedSetpoint": "High", "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "405 AO-TriggerSampleRate": "100000", "470 Shutter-Block voltage": "false", "470-State": "0", "COM3-Parity": "None", "405 AO-Volts": "0.0000", "PRIME-TransposeMirrorY": "0", "PRIME-ChipName": "GS144BSI", "470 AO-Volts": "0.0000", "PRIME-TransposeMirrorX": "0", "445 Shutter-SupportsTriggering": "0", "470 Shutter-SupportsTriggering": "0", "XLIGHT Hub-Description": "XLIGHT hub", "PRIME-PreampOffLimit": "10000", "470 AO-Sequenceable": "1", "528 Shutter-MaxVolts": "5.0000", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "Core-XYStage": "XYStage", "470 AO-TriggerSampleRate": "100000", "PRIME-Timing-PreTriggerDelayNs": "24672000.0000", "528-DA Device": "528 Shutter", "Focus-Description": "Nikon TE2000 focus adapter", "FilterBlock-State": "5", "520 AO-Demo": "No", "COM3-DelayBetweenCharsMs": "0.0000", "470 Shutter-Volts": "0.0000", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "PRIME-PP 2 THRESHOLD": "125", "PRIME-PP 4 THRESHOLD": "75", "XYStage-Acceleration": "4.0000", "470 Shutter-Demo": "No", "PRIME-Name": "PMPCIECam00", "640-DA Device": "640 Shutter", "405 AO-Block voltage": "false", "ElapsedTime-ms": 151193, "PRIME-Offset": "100", "445 AO-IOChannel": "Dev1/ao1", "640 AO-MaxVolts": "5.0000", "PositionIndex": 3, "PRIME-PP 1 ENABLED": "Yes", "COM1-BaudRate": "9600", "640-State": "1", "COM3-Verbose": "1", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "LudlController-TransmissionDelay": "0", "PRIME-FullWellCapacity": "62000", "PRIME-SMARTStreamingEnabled": "No", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "640 Shutter-Description": "NI DAC", "528-Description": "DA device that is used as a shutter", "COM4-Description": "Serial port driver (boost:asio)", "405 Shutter-Description": "NI DAC", "TE2000-Name": "TE2000", "528 Shutter-Description": "NI DAC", "COM9-Description": "Serial port driver (boost:asio)", "LudlController-Name": "LudlController", "445 Shutter-TriggerSequenceLength": "1000", "PRIME-SerialNumber": "A18B203008", "445 AO-Volts": "0.0000", "PRIME-X-dimension": "1200", "PRIME-ShutterCloseDelay": "0", "XLIGHT Disk slider-State": "2", "Position": "Default", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 AO-IOChannel": "Dev1/ao5", "520 AO-IOChannel": "Dev1/ao4", "528 AO-TriggerSequenceLength": "1000", "405 Shutter-MaxVolts": "5.0000", "470-DA Device": "470 Shutter", "PRIME-FTCapable": "No", "Core-ChannelGroup": "Channels", "555 AO-SupportsTriggering": "0", "PRIME-Y-dimension": "1200", "Frame": 0, "555 Shutter-Sequenceable": "1", "PRIME-Timing-ExposureTimeNs": "9992000.0000", "Core-ImageProcessor": "", "528-State": "0", "520 AO-Block voltage": "false", "XLIGHT Excitation Wheel-Label": "QUAD", "640 Shutter-Demo": "No", "COM3-StopBits": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "520 Shutter-IOChannel": "Dev1/ao11", "Width": 1200, "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-SupportsTriggering": "0", "640 AO-TriggerSampleRate": "100000", "520-Description": "DA device that is used as a shutter", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "405 Shutter-Sequenceable": "1", "405 Shutter-SupportsTriggering": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-TriggerSampleRate": "100000", "640 Shutter-MaxVolts": "5.0000", "555 AO-MaxVolts": "5.0000", "520 AO-TriggerSequenceLength": "1000", "COM3-BaudRate": "9600", "COM9-BaudRate": "9600", "Vincent-D3-Channel #": "Ch. #1", "PRIME-PMode": "Normal", "OpticalPath-Label": "Left(100)", "BitDepth": 16, "555 AO-TriggerSequenceLength": "1000", "445 Shutter-Description": "NI DAC", "405 AO-Sequenceable": "1", "PRIME-AcquisitionMethod": "Callbacks", "555 AO-Name": "AnalogIO", "PRIME-ShutterMode": "Pre-Exposure", "COM3-Handshaking": "Off", "445 AO-SupportsTriggering": "0", "ROI": "0-0-1200-1200", "COM9-Verbose": "1", "PRIME-Color": "OFF", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "PRIME-ShutterOpenDelay": "0", "COM1-Parity": "None", "Slice": 0, "SlicePosition": -50, "COM9-DelayBetweenCharsMs": "0.0000", "555-Name": "DA Shutter", "PIEZO-LudlSingleAxisName": "B", "XLIGHT Emission Wheel-Label": "725/40 (C)", "640 Shutter-Name": "AnalogIO", "640 AO-Sequenceable": "1", "520 Shutter-Demo": "No", "528 Shutter-Demo": "No", "470-Name": "DA Shutter", "520-DA Device": "520 Shutter", "XYStage-Description": "Ludl XY stage driver adapter", "445 Shutter-MinVolts": "0.0000", "528 AO-TriggerSampleRate": "100000", "405 AO-MinVolts": "0.0000", "405 Shutter-TriggerSequenceLength": "1000", "Height": 1200, "555 AO-Block voltage": "false", "640 Shutter-Volts": "5.0000", "PixelType": "GRAY16", "445 Shutter-Block voltage": "false", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "520 Shutter-TriggerSequenceLength": "1000", "COM9-Name": "COM9", "Core-SLM": "", "555 AO-Volts": "0.0000", "555 Shutter-Block voltage": "false", "XYStage-Speed": "1800.0000", "520 AO-MaxVolts": "5.0000", "528 Shutter-SupportsTriggering": "0", "COM1-Description": "Serial port driver (boost:asio)", "PRIME-MetadataEnabled": "No", "520 Shutter-MinVolts": "0.0000", "470 Shutter-Sequenceable": "1", "PRIME-PreampDelay": "0", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-MinVolts": "0.0000", "ChannelIndex": 3, "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Core-AutoShutter": "1", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "Exposure-ms": 520, "PRIME-PP 2 ENABLED": "Yes", "640 Shutter-TriggerSampleRate": "100000", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "470 Shutter-IOChannel": "Dev1/ao10", "405 AO-Demo": "No", "PixelSizeUm": 1.1, "470 AO-Demo": "No", "470 Shutter-MaxVolts": "5.0000", "Vincent-D3-Name": "Vincent-D3", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FileName": "img_000000000_Il-C_000.tif", "LudlController-Reset": "Operate", "PRIME-Exposure": "520.00", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "445-Name": "DA Shutter", "555 Shutter-Name": "AnalogIO", "520 Shutter-TriggerSampleRate": "100000", "445 Shutter-IOChannel": "Dev1/ao9", "PRIME-TransposeCorrection": "0", "XYStage-StepSize": "0.1000", "445-Description": "DA device that is used as a shutter", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 AO-IOChannel": "Dev1/ao7", "520 Shutter-Name": "AnalogIO", "COM3-DataBits": "8", "640 AO-Volts": "4.5000", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "555 Shutter-IOChannel": "Dev1/ao13", "WaitInterval": null, "520 Shutter-Sequenceable": "1", "YPositionUm": 20690.300000000003, "445-State": "0", "445-DA Device": "445 Shutter", "PRIME-CircularBufferAutoSize": "ON", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "555 AO-TriggerSampleRate": "100000", "445 Shutter-Volts": "0.0000", "XLIGHT Hub-Port": "COM3", "Core-AutoFocus": "", "COM4-Handshaking": "Off", "528 Shutter-MinVolts": "0.0000", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "445 Shutter-Name": "AnalogIO", "528 Shutter-Sequenceable": "1", "XYStage-TransposeMirrorX": "0", "XYStage-TransposeMirrorY": "0", "PRIME-ReadoutRate": "100MHz 16bit", "XYStage-Name": "Stage", "640 AO-SupportsTriggering": "0", "640-Name": "DA Shutter", "640 Shutter-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "640 AO-Block voltage": "false", "445 Shutter-Sequenceable": "1", "470 AO-MaxVolts": "5.0000", "COM4-BaudRate": "9600", "470-Description": "DA device that is used as a shutter", "520 Shutter-Description": "NI DAC", "PRIME-CCDTemperature": "-14.9600", "Time": "2019-12-17 21:58:58 -0500", "640 AO-TriggerSequenceLength": "1000", "470 AO-Name": "AnalogIO", "XLIGHT Disk slider-Label": "Disk Pos 40um", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "Binning": "1x1", "TE2000-ModelType": "1", "COM3-AnswerTimeout": "500.0000", "555 Shutter-SupportsTriggering": "0", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "TE2000-Port": "COM4", "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Description": "NI DAC", "COM4-AnswerTimeout": "500.0000", "COM1-DelayBetweenCharsMs": "0.0000", "FilterBlock-Label": "Confocal", "445 AO-Name": "AnalogIO", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-Timing-ReadoutTimeNs": "24672000.0000", "528 AO-Demo": "No", "520 AO-Sequenceable": "1", "555-Description": "DA device that is used as a shutter", "405-DA Device": "405 Shutter", "405 AO-TriggerSequenceLength": "1000", "640 AO-MinVolts": "0.0000", "640 Shutter-Block voltage": "false", "FrameIndex": 0, "PRIME-Port": "CMOS", "555 AO-MinVolts": "0.0000", "COM3-Description": "Serial port driver (boost:asio)", "528 AO-Description": "NI DAC", "555 AO-IOChannel": "Dev1/ao6", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "555 AO-TriggerInputLine": "/Dev1/PFI0", "PRIME-ActualInterval-ms": "374.3700", "LudlController-Port": "COM9", "445 Shutter-Demo": "No", "PRIME-Trigger-Expose Out-Mux": "1", "SliceIndex": 0, "PRIME-CCDTemperatureSetPoint": "-15.0000", "405 Shutter-MinVolts": "0.0000", "PRIME-ExposeOutMode": "First Row", "AxisPositions": null, "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Volts": "0.0000", "528-Name": "DA Shutter", "555-State": "0", "XLIGHT Dichroic-Label": "Di03-R635-t1", "445 AO-Sequenceable": "1", "405-Name": "DA Shutter", "405 AO-IOChannel": "Dev1/ao0", "Core-Camera": "PRIME", "Focus-Name": "Focus", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "405 Shutter-IOChannel": "Dev1/ao8", "640 AO-Demo": "No", "640 Shutter-SupportsTriggering": "0", "445 AO-Demo": "No", "555-DA Device": "555 Shutter", "470 Shutter-Description": "NI DAC", "528 AO-Volts": "0.0000", "COM1-DataBits": "8", "Vincent-D3-Address": "x", "520 AO-SupportsTriggering": "0", "405-Description": "DA device that is used as a shutter", "640 Shutter-MinVolts": "0.0000", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "405 AO-Name": "AnalogIO", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "640 AO-TriggerInputLine": "/Dev1/PFI0", "405 AO-TriggerInputLine": "/Dev1/PFI0", "640-Description": "DA device that is used as a shutter", "445 Shutter-TriggerSampleRate": "100000", "470 Shutter-TriggerSequenceLength": "1000", "COM1-StopBits": "1", "520 Shutter-SupportsTriggering": "0", "405-State": "0", "Core-Initialize": "1", "XLIGHT Emission Wheel-State": "7", "520 AO-Description": "NI DAC", "528 AO-TriggerInputLine": "/Dev1/PFI0", "445 Shutter-MaxVolts": "5.0000", "COM9-Handshaking": "Off", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "XLIGHT Spin motor-Label": "On (spinning)", "PRIME-PP 5 ENABLED": "No", "UUID": "fecd123b-cc19-405e-8ce6-1aa83c7361ac", "PRIME-TriggerMode": "Internal Trigger", "COM1-Handshaking": "Off", "PRIME-Timing-PostTriggerDelayNs": "49344000.0000", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "Focus-Position": "16479.8000", "528 Shutter-Block voltage": "false", "Channel": "Il-C", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "555 Shutter-Demo": "No", "405 Shutter-Demo": "No", "528 AO-Name": "AnalogIO", "520-State": "0", "Core-Galvo": "", "405 Shutter-TriggerSampleRate": "100000", "Focus-FocusStepSize_(nm)": "100", "PRIME-PixelType": "16bit", "COM4-DataBits": "8", "470 AO-IOChannel": "Dev1/ao2", "520 AO-MinVolts": "0.0000", "COM9-Parity": "None", "PRIME-CircularBufferEnabled": "ON", "PRIME-PP 6 Reset": "No", "640 AO-Description": "NI DAC", "Source": "PRIME", "445 AO-MinVolts": "0.0000", "PIEZO-Name": "Stage", "445 AO-Description": "NI DAC", "PRIME-CircularBufferFrameCount": "46", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "520 AO-Volts": "0.0000", "528 Shutter-TriggerSampleRate": "100000", "XPositionUm": 62321, "OpticalPath-State": "4", "PRIME-PP 3 THRESHOLD": "80", "PRIME-PVCAM Version": "3.7.1", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Gain": "1-HDR", "640 Shutter-TriggerSequenceLength": "1000", "528 AO-SupportsTriggering": "0", "445 AO-TriggerInputLine": "/Dev1/PFI0", "OpticalPath-Name": "OpticalPath", "PRIME-PP 4 ENABLED": "Yes", "PRIME-PVCAM Adapter Version": "1.3.29", "COM3-Name": "COM3", "405 AO-MaxVolts": "5.0000", "520 Shutter-Block voltage": "false", "PRIME-Timing-ClearingTimeNs": "49344000.0000", "Core-Focus": "PIEZO", "528 AO-Block voltage": "false", "TE2000-Description": "Nikon TE2000 hub", "528 AO-MinVolts": "0.0000", "COM4-Parity": "None", "Summary": { "Slices": 11, "ChContrastMin": [ 0, 0, 0, 0, 0, 0 ], "PixelAspect": 1, "SlicesFirst": true, "Interval_ms": 0, "Time": "2019-12-17 21:58:39 -0500", "Prefix": "seq_3", "InitialPositionList": [ { "Label": "Pos0", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.435], "Focus": [16480.2], "XYStage": [ 69822.40000000001, 20100.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos1", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.425], "Focus": [16480.3], "XYStage": [ 69004, 20101 ] }, "GridColumnIndex": 0 }, { "Label": "Pos2", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.44], "Focus": [16480.1], "XYStage": [ 63119.5, 20690.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos3", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.405], "Focus": [16480.2], "XYStage": [ 62321, 20690.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos4", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.415], "Focus": [16480], "XYStage": [ 56604.100000000006, 20688.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos5", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.42000000000002], "Focus": [16480.1], "XYStage": [ 56034.200000000004, 20688.800000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos6", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.455], "Focus": [16480], "XYStage": [ 50889.3, 20496.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos7", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.43], "Focus": [16480.2], "XYStage": [ 50101.600000000006, 20496.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos8", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.67000000000002], "Focus": [16479.9], "XYStage": [ 44818.600000000006, 20496.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos9", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.67000000000002], "Focus": [16480], "XYStage": [ 43897.9, 20436.800000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos10", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.675], "Focus": [16479.9], "XYStage": [ 39670.600000000006, 20664.9 ] }, "GridColumnIndex": 0 }, { "Label": "Pos11", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.69], "Focus": [16479.9], "XYStage": [ 38734, 20664.600000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos12", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.695], "Focus": [16480.1], "XYStage": [ 34037.200000000004, 20664.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos13", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.685], "Focus": [16480.1], "XYStage": [ 33152, 20664.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos14", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.69], "Focus": [16480.1], "XYStage": [ 28122.800000000003, 20664.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos15", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.675], "Focus": [16480], "XYStage": [ 27422.9, 20664.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos16", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.64000000000001], "Focus": [16480], "XYStage": [ 23005.2, 20664.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos17", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.69], "Focus": [16479.9], "XYStage": [ 21820.600000000002, 20409.600000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos18", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.41], "Focus": [16480.1], "XYStage": [ 73697.6, -13222.800000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos19", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.455], "Focus": [16480.1], "XYStage": [ 72765.8, -13223 ] }, "GridColumnIndex": 0 }, { "Label": "Pos20", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.4], "Focus": [16480.2], "XYStage": [ 67445.5, -12303.800000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos21", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.415], "Focus": [16480.2], "XYStage": [ 66613.3, -12303.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos22", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.415], "Focus": [16480.1], "XYStage": [ 61819.4, -11936.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos23", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.405], "Focus": [16480.2], "XYStage": [ 61325.700000000004, -11937.1 ] }, "GridColumnIndex": 0 }, { "Label": "Pos24", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.46], "Focus": [16480.2], "XYStage": [ 55377, -10974.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos25", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.445], "Focus": [16480.1], "XYStage": [ 54827, -11214.800000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos26", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.435], "Focus": [16480.2], "XYStage": [ 49937.100000000006, -10919.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos27", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.425], "Focus": [16480.1], "XYStage": [ 48764, -10919.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos28", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.42000000000002], "Focus": [16480.2], "XYStage": [ 43978.4, -10920.400000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos29", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.435], "Focus": [16480.2], "XYStage": [ 43106.3, -10920.400000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos30", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.405], "Focus": [16480.3], "XYStage": [ 38212.4, -10920.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos31", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.445], "Focus": [16480.2], "XYStage": [ 37254.9, -10920.400000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos32", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.44], "Focus": [16480.3], "XYStage": [ 32472.800000000003, -11294.400000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos33", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [166.95000000000002], "Focus": [16480.2], "XYStage": [ 31420.7, -11294.400000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos34", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [166.95000000000002], "Focus": [16480.2], "XYStage": [ 26881.5, -11640 ] }, "GridColumnIndex": 0 }, { "Label": "Pos35", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [166.98], "Focus": [16480.2], "XYStage": [ 25876.100000000002, -11641.1 ] }, "GridColumnIndex": 0 } ], "z-step_um": 10, "GridRow": 0, "BitDepth": 16, "Depth": 2, "Channels": 6, "Source": "Micro-Manager", "ChColors": [ -16711681, -256, -65281, -1, -1, -16776961 ], "MetadataVersion": 10, "KeepShutterOpenChannels": true, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536, 65536 ], "CustomIntervals_ms": [], "Height": 1200, "MicroManagerVersion": "1.4.23 20180219", "GridColumn": 0, "UUID": "8c8f9d20-b128-4577-9f91-89e70f22d319", "PixelType": "GRAY16", "Width": 1200, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\Yan\\20191217-YC113-S5S6", "Frames": 1, "Comment": "", "UserName": "Nikon", "PixelSize_um": 1.1, "ROI": [ 0, 0, 1200, 1200 ], "Date": "2019-12-17", "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC", "P-DAPI-high" ], "KeepShutterOpenSlices": true, "IJType": 1, "ComputerName": "ZadorConfocal2", "Positions": 36, "PositionIndex": 3, "CameraTimeout": 20000, "TimeFirst": false }, "640 AO-Name": "AnalogIO", "Vincent-D3-Command": "Close" }, "FrameKey-0-3-1": { "COM4-StopBits": "1", "528 AO-MaxVolts": "5.0000", "XLIGHT Excitation Wheel-State": "2", "470 Shutter-Name": "AnalogIO", "Core-Shutter": "640", "555 AO-Demo": "No", "XLIGHT Dichroic-State": "2", "XYStage-StepSize-Y": "0.1000", "ZPositionUm": 137.405, "XYStage-StepSize-X": "0.1000", "PRIME-FirmwareVersion": "20.28", "PRIME-ClearCycles": "2", "520-Name": "DA Shutter", "405 Shutter-Name": "AnalogIO", "PIEZO-StepSize": "0.0050", "PRIME-ClearMode": "Pre-Exposure", "470 AO-MinVolts": "0.0000", "405 AO-SupportsTriggering": "1", "CameraChannelIndex": 0, "XYStage-StartSpeed": "500.0000", "COM9-StopBits": "2", "405 AO-Description": "NI DAC", "COM4-DelayBetweenCharsMs": "0.0000", "445 AO-Block voltage": "false", "520 AO-TriggerInputLine": "/Dev1/PFI0", "555 AO-Description": "NI DAC", "520 AO-TriggerSampleRate": "100000", "COM4-Verbose": "1", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM4-Name": "COM4", "405 Shutter-Volts": "0.0000", "Vincent-D3-Port": "COM1", "PRIME-PP 4 MIN ADU AFFECTED": "200", "470 Shutter-MinVolts": "0.0000", "PRIME-PP 3 ENABLED": "Yes", "470 AO-Description": "NI DAC", "PRIME-Description": "PVCAM API device adapter", "520 Shutter-MaxVolts": "5.0000", "520 Shutter-Volts": "0.0000", "528 AO-Sequenceable": "1", "PRIME-Binning": "1x1", "555 Shutter-MaxVolts": "5.0000", "528 Shutter-TriggerSequenceLength": "1000", "405 Shutter-Block voltage": "false", "XLIGHT Spin motor-State": "1", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "Vincent-D3-Time to open (ms)": "35.0000", "Vincent-D3-Time to close (ms)": "35.0000", "445 AO-TriggerSampleRate": "100000", "640 Shutter-IOChannel": "Dev1/ao14", "555 AO-Sequenceable": "1", "520 AO-Name": "AnalogIO", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "470 AO-Block voltage": "false", "COM1-Verbose": "1", "COM9-AnswerTimeout": "10000.0000", "528 Shutter-Name": "AnalogIO", "PRIME-Trigger Timeout (secs)": "10", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "NextFrame": 0, "470 AO-TriggerSequenceLength": "1000", "PRIME-TransposeXY": "0", "445 AO-TriggerSequenceLength": "1000", "COM1-Name": "COM1", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "COM9-DataBits": "8", "COM1-AnswerTimeout": "500.0000", "Camera": "", "TE2000-Version": "V2.50-V2.50-V2.10", "528 Shutter-Volts": "0.0000", "XLIGHT Hub-Name": "XLIGHT Hub", "PIEZO-Description": "Ludl stage driver adapter", "PositionName": "Pos3", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSampleRate": "100000", "PRIME-FanSpeedSetpoint": "High", "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "405 AO-TriggerSampleRate": "100000", "470 Shutter-Block voltage": "false", "470-State": "0", "COM3-Parity": "None", "405 AO-Volts": "0.0000", "PRIME-TransposeMirrorY": "0", "PRIME-ChipName": "GS144BSI", "470 AO-Volts": "0.0000", "PRIME-TransposeMirrorX": "0", "445 Shutter-SupportsTriggering": "0", "470 Shutter-SupportsTriggering": "0", "XLIGHT Hub-Description": "XLIGHT hub", "PRIME-PreampOffLimit": "10000", "470 AO-Sequenceable": "1", "528 Shutter-MaxVolts": "5.0000", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "Core-XYStage": "XYStage", "470 AO-TriggerSampleRate": "100000", "PRIME-Timing-PreTriggerDelayNs": "24672000.0000", "528-DA Device": "528 Shutter", "Focus-Description": "Nikon TE2000 focus adapter", "FilterBlock-State": "5", "520 AO-Demo": "No", "COM3-DelayBetweenCharsMs": "0.0000", "470 Shutter-Volts": "0.0000", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "PRIME-PP 2 THRESHOLD": "125", "PRIME-PP 4 THRESHOLD": "75", "XYStage-Acceleration": "4.0000", "470 Shutter-Demo": "No", "PRIME-Name": "PMPCIECam00", "640-DA Device": "640 Shutter", "405 AO-Block voltage": "false", "ElapsedTime-ms": 151955, "PRIME-Offset": "100", "445 AO-IOChannel": "Dev1/ao1", "640 AO-MaxVolts": "5.0000", "PositionIndex": 3, "PRIME-PP 1 ENABLED": "Yes", "COM1-BaudRate": "9600", "640-State": "1", "COM3-Verbose": "1", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "LudlController-TransmissionDelay": "0", "PRIME-FullWellCapacity": "62000", "PRIME-SMARTStreamingEnabled": "No", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "640 Shutter-Description": "NI DAC", "528-Description": "DA device that is used as a shutter", "COM4-Description": "Serial port driver (boost:asio)", "405 Shutter-Description": "NI DAC", "TE2000-Name": "TE2000", "528 Shutter-Description": "NI DAC", "COM9-Description": "Serial port driver (boost:asio)", "LudlController-Name": "LudlController", "445 Shutter-TriggerSequenceLength": "1000", "PRIME-SerialNumber": "A18B203008", "445 AO-Volts": "0.0000", "PRIME-X-dimension": "1200", "PRIME-ShutterCloseDelay": "0", "XLIGHT Disk slider-State": "2", "Position": "Default", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 AO-IOChannel": "Dev1/ao5", "520 AO-IOChannel": "Dev1/ao4", "528 AO-TriggerSequenceLength": "1000", "405 Shutter-MaxVolts": "5.0000", "470-DA Device": "470 Shutter", "PRIME-FTCapable": "No", "Core-ChannelGroup": "Channels", "555 AO-SupportsTriggering": "0", "PRIME-Y-dimension": "1200", "Frame": 0, "555 Shutter-Sequenceable": "1", "PRIME-Timing-ExposureTimeNs": "9992000.0000", "Core-ImageProcessor": "", "528-State": "0", "520 AO-Block voltage": "false", "XLIGHT Excitation Wheel-Label": "QUAD", "640 Shutter-Demo": "No", "COM3-StopBits": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "520 Shutter-IOChannel": "Dev1/ao11", "Width": 1200, "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-SupportsTriggering": "0", "640 AO-TriggerSampleRate": "100000", "520-Description": "DA device that is used as a shutter", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "405 Shutter-Sequenceable": "1", "405 Shutter-SupportsTriggering": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-TriggerSampleRate": "100000", "640 Shutter-MaxVolts": "5.0000", "555 AO-MaxVolts": "5.0000", "520 AO-TriggerSequenceLength": "1000", "COM3-BaudRate": "9600", "COM9-BaudRate": "9600", "Vincent-D3-Channel #": "Ch. #1", "PRIME-PMode": "Normal", "OpticalPath-Label": "Left(100)", "BitDepth": 16, "555 AO-TriggerSequenceLength": "1000", "445 Shutter-Description": "NI DAC", "405 AO-Sequenceable": "1", "PRIME-AcquisitionMethod": "Callbacks", "555 AO-Name": "AnalogIO", "PRIME-ShutterMode": "Pre-Exposure", "COM3-Handshaking": "Off", "445 AO-SupportsTriggering": "0", "ROI": "0-0-1200-1200", "COM9-Verbose": "1", "PRIME-Color": "OFF", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "PRIME-ShutterOpenDelay": "0", "COM1-Parity": "None", "Slice": 1, "SlicePosition": -40, "COM9-DelayBetweenCharsMs": "0.0000", "555-Name": "DA Shutter", "PIEZO-LudlSingleAxisName": "B", "XLIGHT Emission Wheel-Label": "725/40 (C)", "640 Shutter-Name": "AnalogIO", "640 AO-Sequenceable": "1", "520 Shutter-Demo": "No", "528 Shutter-Demo": "No", "470-Name": "DA Shutter", "520-DA Device": "520 Shutter", "XYStage-Description": "Ludl XY stage driver adapter", "445 Shutter-MinVolts": "0.0000", "528 AO-TriggerSampleRate": "100000", "405 AO-MinVolts": "0.0000", "405 Shutter-TriggerSequenceLength": "1000", "Height": 1200, "555 AO-Block voltage": "false", "640 Shutter-Volts": "5.0000", "PixelType": "GRAY16", "445 Shutter-Block voltage": "false", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "520 Shutter-TriggerSequenceLength": "1000", "COM9-Name": "COM9", "Core-SLM": "", "555 AO-Volts": "0.0000", "555 Shutter-Block voltage": "false", "XYStage-Speed": "1800.0000", "520 AO-MaxVolts": "5.0000", "528 Shutter-SupportsTriggering": "0", "COM1-Description": "Serial port driver (boost:asio)", "PRIME-MetadataEnabled": "No", "520 Shutter-MinVolts": "0.0000", "470 Shutter-Sequenceable": "1", "PRIME-PreampDelay": "0", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-MinVolts": "0.0000", "ChannelIndex": 3, "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Core-AutoShutter": "1", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "Exposure-ms": 520, "PRIME-PP 2 ENABLED": "Yes", "640 Shutter-TriggerSampleRate": "100000", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "470 Shutter-IOChannel": "Dev1/ao10", "405 AO-Demo": "No", "PixelSizeUm": 1.1, "470 AO-Demo": "No", "470 Shutter-MaxVolts": "5.0000", "Vincent-D3-Name": "Vincent-D3", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FileName": "img_000000000_Il-C_001.tif", "LudlController-Reset": "Operate", "PRIME-Exposure": "520.00", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "445-Name": "DA Shutter", "555 Shutter-Name": "AnalogIO", "520 Shutter-TriggerSampleRate": "100000", "445 Shutter-IOChannel": "Dev1/ao9", "PRIME-TransposeCorrection": "0", "XYStage-StepSize": "0.1000", "445-Description": "DA device that is used as a shutter", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 AO-IOChannel": "Dev1/ao7", "520 Shutter-Name": "AnalogIO", "COM3-DataBits": "8", "640 AO-Volts": "4.5000", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "555 Shutter-IOChannel": "Dev1/ao13", "WaitInterval": null, "520 Shutter-Sequenceable": "1", "YPositionUm": 20690.300000000003, "445-State": "0", "445-DA Device": "445 Shutter", "PRIME-CircularBufferAutoSize": "ON", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "555 AO-TriggerSampleRate": "100000", "445 Shutter-Volts": "0.0000", "XLIGHT Hub-Port": "COM3", "Core-AutoFocus": "", "COM4-Handshaking": "Off", "528 Shutter-MinVolts": "0.0000", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "445 Shutter-Name": "AnalogIO", "528 Shutter-Sequenceable": "1", "XYStage-TransposeMirrorX": "0", "XYStage-TransposeMirrorY": "0", "PRIME-ReadoutRate": "100MHz 16bit", "XYStage-Name": "Stage", "640 AO-SupportsTriggering": "0", "640-Name": "DA Shutter", "640 Shutter-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "640 AO-Block voltage": "false", "445 Shutter-Sequenceable": "1", "470 AO-MaxVolts": "5.0000", "COM4-BaudRate": "9600", "470-Description": "DA device that is used as a shutter", "520 Shutter-Description": "NI DAC", "PRIME-CCDTemperature": "-14.9600", "Time": "2019-12-17 21:58:59 -0500", "640 AO-TriggerSequenceLength": "1000", "470 AO-Name": "AnalogIO", "XLIGHT Disk slider-Label": "Disk Pos 40um", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "Binning": "1x1", "TE2000-ModelType": "1", "COM3-AnswerTimeout": "500.0000", "555 Shutter-SupportsTriggering": "0", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "TE2000-Port": "COM4", "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Description": "NI DAC", "COM4-AnswerTimeout": "500.0000", "COM1-DelayBetweenCharsMs": "0.0000", "FilterBlock-Label": "Confocal", "445 AO-Name": "AnalogIO", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-Timing-ReadoutTimeNs": "24672000.0000", "528 AO-Demo": "No", "520 AO-Sequenceable": "1", "555-Description": "DA device that is used as a shutter", "405-DA Device": "405 Shutter", "405 AO-TriggerSequenceLength": "1000", "640 AO-MinVolts": "0.0000", "640 Shutter-Block voltage": "false", "FrameIndex": 0, "PRIME-Port": "CMOS", "555 AO-MinVolts": "0.0000", "COM3-Description": "Serial port driver (boost:asio)", "528 AO-Description": "NI DAC", "555 AO-IOChannel": "Dev1/ao6", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "555 AO-TriggerInputLine": "/Dev1/PFI0", "PRIME-ActualInterval-ms": "374.3700", "LudlController-Port": "COM9", "445 Shutter-Demo": "No", "PRIME-Trigger-Expose Out-Mux": "1", "SliceIndex": 1, "PRIME-CCDTemperatureSetPoint": "-15.0000", "405 Shutter-MinVolts": "0.0000", "PRIME-ExposeOutMode": "First Row", "AxisPositions": null, "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Volts": "0.0000", "528-Name": "DA Shutter", "555-State": "0", "XLIGHT Dichroic-Label": "Di03-R635-t1", "445 AO-Sequenceable": "1", "405-Name": "DA Shutter", "405 AO-IOChannel": "Dev1/ao0", "Core-Camera": "PRIME", "Focus-Name": "Focus", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "405 Shutter-IOChannel": "Dev1/ao8", "640 AO-Demo": "No", "640 Shutter-SupportsTriggering": "0", "445 AO-Demo": "No", "555-DA Device": "555 Shutter", "470 Shutter-Description": "NI DAC", "528 AO-Volts": "0.0000", "COM1-DataBits": "8", "Vincent-D3-Address": "x", "520 AO-SupportsTriggering": "0", "405-Description": "DA device that is used as a shutter", "640 Shutter-MinVolts": "0.0000", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "405 AO-Name": "AnalogIO", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "640 AO-TriggerInputLine": "/Dev1/PFI0", "405 AO-TriggerInputLine": "/Dev1/PFI0", "640-Description": "DA device that is used as a shutter", "445 Shutter-TriggerSampleRate": "100000", "470 Shutter-TriggerSequenceLength": "1000", "COM1-StopBits": "1", "520 Shutter-SupportsTriggering": "0", "405-State": "0", "Core-Initialize": "1", "XLIGHT Emission Wheel-State": "7", "520 AO-Description": "NI DAC", "528 AO-TriggerInputLine": "/Dev1/PFI0", "445 Shutter-MaxVolts": "5.0000", "COM9-Handshaking": "Off", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "XLIGHT Spin motor-Label": "On (spinning)", "PRIME-PP 5 ENABLED": "No", "UUID": "98bcd301-ef3d-4c67-8924-e378da62450a", "PRIME-TriggerMode": "Internal Trigger", "COM1-Handshaking": "Off", "PRIME-Timing-PostTriggerDelayNs": "49344000.0000", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "Focus-Position": "16479.8000", "528 Shutter-Block voltage": "false", "Channel": "Il-C", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "555 Shutter-Demo": "No", "405 Shutter-Demo": "No", "528 AO-Name": "AnalogIO", "520-State": "0", "Core-Galvo": "", "405 Shutter-TriggerSampleRate": "100000", "Focus-FocusStepSize_(nm)": "100", "PRIME-PixelType": "16bit", "COM4-DataBits": "8", "470 AO-IOChannel": "Dev1/ao2", "520 AO-MinVolts": "0.0000", "COM9-Parity": "None", "PRIME-CircularBufferEnabled": "ON", "PRIME-PP 6 Reset": "No", "640 AO-Description": "NI DAC", "Source": "PRIME", "445 AO-MinVolts": "0.0000", "PIEZO-Name": "Stage", "445 AO-Description": "NI DAC", "PRIME-CircularBufferFrameCount": "46", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "520 AO-Volts": "0.0000", "528 Shutter-TriggerSampleRate": "100000", "XPositionUm": 62321, "OpticalPath-State": "4", "PRIME-PP 3 THRESHOLD": "80", "PRIME-PVCAM Version": "3.7.1", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Gain": "1-HDR", "640 Shutter-TriggerSequenceLength": "1000", "528 AO-SupportsTriggering": "0", "445 AO-TriggerInputLine": "/Dev1/PFI0", "OpticalPath-Name": "OpticalPath", "PRIME-PP 4 ENABLED": "Yes", "PRIME-PVCAM Adapter Version": "1.3.29", "COM3-Name": "COM3", "405 AO-MaxVolts": "5.0000", "520 Shutter-Block voltage": "false", "PRIME-Timing-ClearingTimeNs": "49344000.0000", "Core-Focus": "PIEZO", "528 AO-Block voltage": "false", "TE2000-Description": "Nikon TE2000 hub", "528 AO-MinVolts": "0.0000", "COM4-Parity": "None", "Summary": { "Slices": 11, "ChContrastMin": [ 0, 0, 0, 0, 0, 0 ], "PixelAspect": 1, "SlicesFirst": true, "Interval_ms": 0, "Time": "2019-12-17 21:58:39 -0500", "Prefix": "seq_3", "InitialPositionList": [ { "Label": "Pos0", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.435], "Focus": [16480.2], "XYStage": [ 69822.40000000001, 20100.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos1", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.425], "Focus": [16480.3], "XYStage": [ 69004, 20101 ] }, "GridColumnIndex": 0 }, { "Label": "Pos2", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.44], "Focus": [16480.1], "XYStage": [ 63119.5, 20690.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos3", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.405], "Focus": [16480.2], "XYStage": [ 62321, 20690.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos4", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.415], "Focus": [16480], "XYStage": [ 56604.100000000006, 20688.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos5", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.42000000000002], "Focus": [16480.1], "XYStage": [ 56034.200000000004, 20688.800000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos6", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.455], "Focus": [16480], "XYStage": [ 50889.3, 20496.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos7", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.43], "Focus": [16480.2], "XYStage": [ 50101.600000000006, 20496.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos8", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.67000000000002], "Focus": [16479.9], "XYStage": [ 44818.600000000006, 20496.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos9", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.67000000000002], "Focus": [16480], "XYStage": [ 43897.9, 20436.800000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos10", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.675], "Focus": [16479.9], "XYStage": [ 39670.600000000006, 20664.9 ] }, "GridColumnIndex": 0 }, { "Label": "Pos11", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.69], "Focus": [16479.9], "XYStage": [ 38734, 20664.600000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos12", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.695], "Focus": [16480.1], "XYStage": [ 34037.200000000004, 20664.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos13", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.685], "Focus": [16480.1], "XYStage": [ 33152, 20664.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos14", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.69], "Focus": [16480.1], "XYStage": [ 28122.800000000003, 20664.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos15", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.675], "Focus": [16480], "XYStage": [ 27422.9, 20664.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos16", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.64000000000001], "Focus": [16480], "XYStage": [ 23005.2, 20664.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos17", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.69], "Focus": [16479.9], "XYStage": [ 21820.600000000002, 20409.600000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos18", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.41], "Focus": [16480.1], "XYStage": [ 73697.6, -13222.800000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos19", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.455], "Focus": [16480.1], "XYStage": [ 72765.8, -13223 ] }, "GridColumnIndex": 0 }, { "Label": "Pos20", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.4], "Focus": [16480.2], "XYStage": [ 67445.5, -12303.800000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos21", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.415], "Focus": [16480.2], "XYStage": [ 66613.3, -12303.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos22", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.415], "Focus": [16480.1], "XYStage": [ 61819.4, -11936.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos23", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.405], "Focus": [16480.2], "XYStage": [ 61325.700000000004, -11937.1 ] }, "GridColumnIndex": 0 }, { "Label": "Pos24", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.46], "Focus": [16480.2], "XYStage": [ 55377, -10974.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos25", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.445], "Focus": [16480.1], "XYStage": [ 54827, -11214.800000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos26", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.435], "Focus": [16480.2], "XYStage": [ 49937.100000000006, -10919.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos27", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.425], "Focus": [16480.1], "XYStage": [ 48764, -10919.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos28", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.42000000000002], "Focus": [16480.2], "XYStage": [ 43978.4, -10920.400000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos29", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.435], "Focus": [16480.2], "XYStage": [ 43106.3, -10920.400000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos30", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.405], "Focus": [16480.3], "XYStage": [ 38212.4, -10920.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos31", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.445], "Focus": [16480.2], "XYStage": [ 37254.9, -10920.400000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos32", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.44], "Focus": [16480.3], "XYStage": [ 32472.800000000003, -11294.400000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos33", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [166.95000000000002], "Focus": [16480.2], "XYStage": [ 31420.7, -11294.400000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos34", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [166.95000000000002], "Focus": [16480.2], "XYStage": [ 26881.5, -11640 ] }, "GridColumnIndex": 0 }, { "Label": "Pos35", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [166.98], "Focus": [16480.2], "XYStage": [ 25876.100000000002, -11641.1 ] }, "GridColumnIndex": 0 } ], "z-step_um": 10, "GridRow": 0, "BitDepth": 16, "Depth": 2, "Channels": 6, "Source": "Micro-Manager", "ChColors": [ -16711681, -256, -65281, -1, -1, -16776961 ], "MetadataVersion": 10, "KeepShutterOpenChannels": true, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536, 65536 ], "CustomIntervals_ms": [], "Height": 1200, "MicroManagerVersion": "1.4.23 20180219", "GridColumn": 0, "UUID": "8c8f9d20-b128-4577-9f91-89e70f22d319", "PixelType": "GRAY16", "Width": 1200, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\Yan\\20191217-YC113-S5S6", "Frames": 1, "Comment": "", "UserName": "Nikon", "PixelSize_um": 1.1, "ROI": [ 0, 0, 1200, 1200 ], "Date": "2019-12-17", "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC", "P-DAPI-high" ], "KeepShutterOpenSlices": true, "IJType": 1, "ComputerName": "ZadorConfocal2", "Positions": 36, "PositionIndex": 3, "CameraTimeout": 20000, "TimeFirst": false }, "640 AO-Name": "AnalogIO", "Vincent-D3-Command": "Close" }, "FrameKey-0-3-2": { "COM4-StopBits": "1", "528 AO-MaxVolts": "5.0000", "XLIGHT Excitation Wheel-State": "2", "470 Shutter-Name": "AnalogIO", "Core-Shutter": "640", "555 AO-Demo": "No", "XLIGHT Dichroic-State": "2", "XYStage-StepSize-Y": "0.1000", "ZPositionUm": 147.405, "XYStage-StepSize-X": "0.1000", "PRIME-FirmwareVersion": "20.28", "PRIME-ClearCycles": "2", "520-Name": "DA Shutter", "405 Shutter-Name": "AnalogIO", "PIEZO-StepSize": "0.0050", "PRIME-ClearMode": "Pre-Exposure", "470 AO-MinVolts": "0.0000", "405 AO-SupportsTriggering": "1", "CameraChannelIndex": 0, "XYStage-StartSpeed": "500.0000", "COM9-StopBits": "2", "405 AO-Description": "NI DAC", "COM4-DelayBetweenCharsMs": "0.0000", "445 AO-Block voltage": "false", "520 AO-TriggerInputLine": "/Dev1/PFI0", "555 AO-Description": "NI DAC", "520 AO-TriggerSampleRate": "100000", "COM4-Verbose": "1", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM4-Name": "COM4", "405 Shutter-Volts": "0.0000", "Vincent-D3-Port": "COM1", "PRIME-PP 4 MIN ADU AFFECTED": "200", "470 Shutter-MinVolts": "0.0000", "PRIME-PP 3 ENABLED": "Yes", "470 AO-Description": "NI DAC", "PRIME-Description": "PVCAM API device adapter", "520 Shutter-MaxVolts": "5.0000", "520 Shutter-Volts": "0.0000", "528 AO-Sequenceable": "1", "PRIME-Binning": "1x1", "555 Shutter-MaxVolts": "5.0000", "528 Shutter-TriggerSequenceLength": "1000", "405 Shutter-Block voltage": "false", "XLIGHT Spin motor-State": "1", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "Vincent-D3-Time to open (ms)": "35.0000", "Vincent-D3-Time to close (ms)": "35.0000", "445 AO-TriggerSampleRate": "100000", "640 Shutter-IOChannel": "Dev1/ao14", "555 AO-Sequenceable": "1", "520 AO-Name": "AnalogIO", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "470 AO-Block voltage": "false", "COM1-Verbose": "1", "COM9-AnswerTimeout": "10000.0000", "528 Shutter-Name": "AnalogIO", "PRIME-Trigger Timeout (secs)": "10", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "NextFrame": 0, "470 AO-TriggerSequenceLength": "1000", "PRIME-TransposeXY": "0", "445 AO-TriggerSequenceLength": "1000", "COM1-Name": "COM1", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "COM9-DataBits": "8", "COM1-AnswerTimeout": "500.0000", "Camera": "", "TE2000-Version": "V2.50-V2.50-V2.10", "528 Shutter-Volts": "0.0000", "XLIGHT Hub-Name": "XLIGHT Hub", "PIEZO-Description": "Ludl stage driver adapter", "PositionName": "Pos3", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSampleRate": "100000", "PRIME-FanSpeedSetpoint": "High", "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "405 AO-TriggerSampleRate": "100000", "470 Shutter-Block voltage": "false", "470-State": "0", "COM3-Parity": "None", "405 AO-Volts": "0.0000", "PRIME-TransposeMirrorY": "0", "PRIME-ChipName": "GS144BSI", "470 AO-Volts": "0.0000", "PRIME-TransposeMirrorX": "0", "445 Shutter-SupportsTriggering": "0", "470 Shutter-SupportsTriggering": "0", "XLIGHT Hub-Description": "XLIGHT hub", "PRIME-PreampOffLimit": "10000", "470 AO-Sequenceable": "1", "528 Shutter-MaxVolts": "5.0000", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "Core-XYStage": "XYStage", "470 AO-TriggerSampleRate": "100000", "PRIME-Timing-PreTriggerDelayNs": "24672000.0000", "528-DA Device": "528 Shutter", "Focus-Description": "Nikon TE2000 focus adapter", "FilterBlock-State": "5", "520 AO-Demo": "No", "COM3-DelayBetweenCharsMs": "0.0000", "470 Shutter-Volts": "0.0000", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "PRIME-PP 2 THRESHOLD": "125", "PRIME-PP 4 THRESHOLD": "75", "XYStage-Acceleration": "4.0000", "470 Shutter-Demo": "No", "PRIME-Name": "PMPCIECam00", "640-DA Device": "640 Shutter", "405 AO-Block voltage": "false", "ElapsedTime-ms": 152713, "PRIME-Offset": "100", "445 AO-IOChannel": "Dev1/ao1", "640 AO-MaxVolts": "5.0000", "PositionIndex": 3, "PRIME-PP 1 ENABLED": "Yes", "COM1-BaudRate": "9600", "640-State": "1", "COM3-Verbose": "1", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "LudlController-TransmissionDelay": "0", "PRIME-FullWellCapacity": "62000", "PRIME-SMARTStreamingEnabled": "No", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "640 Shutter-Description": "NI DAC", "528-Description": "DA device that is used as a shutter", "COM4-Description": "Serial port driver (boost:asio)", "405 Shutter-Description": "NI DAC", "TE2000-Name": "TE2000", "528 Shutter-Description": "NI DAC", "COM9-Description": "Serial port driver (boost:asio)", "LudlController-Name": "LudlController", "445 Shutter-TriggerSequenceLength": "1000", "PRIME-SerialNumber": "A18B203008", "445 AO-Volts": "0.0000", "PRIME-X-dimension": "1200", "PRIME-ShutterCloseDelay": "0", "XLIGHT Disk slider-State": "2", "Position": "Default", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 AO-IOChannel": "Dev1/ao5", "520 AO-IOChannel": "Dev1/ao4", "528 AO-TriggerSequenceLength": "1000", "405 Shutter-MaxVolts": "5.0000", "470-DA Device": "470 Shutter", "PRIME-FTCapable": "No", "Core-ChannelGroup": "Channels", "555 AO-SupportsTriggering": "0", "PRIME-Y-dimension": "1200", "Frame": 0, "555 Shutter-Sequenceable": "1", "PRIME-Timing-ExposureTimeNs": "9992000.0000", "Core-ImageProcessor": "", "528-State": "0", "520 AO-Block voltage": "false", "XLIGHT Excitation Wheel-Label": "QUAD", "640 Shutter-Demo": "No", "COM3-StopBits": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "520 Shutter-IOChannel": "Dev1/ao11", "Width": 1200, "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-SupportsTriggering": "0", "640 AO-TriggerSampleRate": "100000", "520-Description": "DA device that is used as a shutter", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "405 Shutter-Sequenceable": "1", "405 Shutter-SupportsTriggering": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-TriggerSampleRate": "100000", "640 Shutter-MaxVolts": "5.0000", "555 AO-MaxVolts": "5.0000", "520 AO-TriggerSequenceLength": "1000", "COM3-BaudRate": "9600", "COM9-BaudRate": "9600", "Vincent-D3-Channel #": "Ch. #1", "PRIME-PMode": "Normal", "OpticalPath-Label": "Left(100)", "BitDepth": 16, "555 AO-TriggerSequenceLength": "1000", "445 Shutter-Description": "NI DAC", "405 AO-Sequenceable": "1", "PRIME-AcquisitionMethod": "Callbacks", "555 AO-Name": "AnalogIO", "PRIME-ShutterMode": "Pre-Exposure", "COM3-Handshaking": "Off", "445 AO-SupportsTriggering": "0", "ROI": "0-0-1200-1200", "COM9-Verbose": "1", "PRIME-Color": "OFF", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "PRIME-ShutterOpenDelay": "0", "COM1-Parity": "None", "Slice": 2, "SlicePosition": -30, "COM9-DelayBetweenCharsMs": "0.0000", "555-Name": "DA Shutter", "PIEZO-LudlSingleAxisName": "B", "XLIGHT Emission Wheel-Label": "725/40 (C)", "640 Shutter-Name": "AnalogIO", "640 AO-Sequenceable": "1", "520 Shutter-Demo": "No", "528 Shutter-Demo": "No", "470-Name": "DA Shutter", "520-DA Device": "520 Shutter", "XYStage-Description": "Ludl XY stage driver adapter", "445 Shutter-MinVolts": "0.0000", "528 AO-TriggerSampleRate": "100000", "405 AO-MinVolts": "0.0000", "405 Shutter-TriggerSequenceLength": "1000", "Height": 1200, "555 AO-Block voltage": "false", "640 Shutter-Volts": "5.0000", "PixelType": "GRAY16", "445 Shutter-Block voltage": "false", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "520 Shutter-TriggerSequenceLength": "1000", "COM9-Name": "COM9", "Core-SLM": "", "555 AO-Volts": "0.0000", "555 Shutter-Block voltage": "false", "XYStage-Speed": "1800.0000", "520 AO-MaxVolts": "5.0000", "528 Shutter-SupportsTriggering": "0", "COM1-Description": "Serial port driver (boost:asio)", "PRIME-MetadataEnabled": "No", "520 Shutter-MinVolts": "0.0000", "470 Shutter-Sequenceable": "1", "PRIME-PreampDelay": "0", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-MinVolts": "0.0000", "ChannelIndex": 3, "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Core-AutoShutter": "1", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "Exposure-ms": 520, "PRIME-PP 2 ENABLED": "Yes", "640 Shutter-TriggerSampleRate": "100000", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "470 Shutter-IOChannel": "Dev1/ao10", "405 AO-Demo": "No", "PixelSizeUm": 1.1, "470 AO-Demo": "No", "470 Shutter-MaxVolts": "5.0000", "Vincent-D3-Name": "Vincent-D3", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FileName": "img_000000000_Il-C_002.tif", "LudlController-Reset": "Operate", "PRIME-Exposure": "520.00", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "445-Name": "DA Shutter", "555 Shutter-Name": "AnalogIO", "520 Shutter-TriggerSampleRate": "100000", "445 Shutter-IOChannel": "Dev1/ao9", "PRIME-TransposeCorrection": "0", "XYStage-StepSize": "0.1000", "445-Description": "DA device that is used as a shutter", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 AO-IOChannel": "Dev1/ao7", "520 Shutter-Name": "AnalogIO", "COM3-DataBits": "8", "640 AO-Volts": "4.5000", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "555 Shutter-IOChannel": "Dev1/ao13", "WaitInterval": null, "520 Shutter-Sequenceable": "1", "YPositionUm": 20690.300000000003, "445-State": "0", "445-DA Device": "445 Shutter", "PRIME-CircularBufferAutoSize": "ON", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "555 AO-TriggerSampleRate": "100000", "445 Shutter-Volts": "0.0000", "XLIGHT Hub-Port": "COM3", "Core-AutoFocus": "", "COM4-Handshaking": "Off", "528 Shutter-MinVolts": "0.0000", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "445 Shutter-Name": "AnalogIO", "528 Shutter-Sequenceable": "1", "XYStage-TransposeMirrorX": "0", "XYStage-TransposeMirrorY": "0", "PRIME-ReadoutRate": "100MHz 16bit", "XYStage-Name": "Stage", "640 AO-SupportsTriggering": "0", "640-Name": "DA Shutter", "640 Shutter-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "640 AO-Block voltage": "false", "445 Shutter-Sequenceable": "1", "470 AO-MaxVolts": "5.0000", "COM4-BaudRate": "9600", "470-Description": "DA device that is used as a shutter", "520 Shutter-Description": "NI DAC", "PRIME-CCDTemperature": "-14.9600", "Time": "2019-12-17 21:59:00 -0500", "640 AO-TriggerSequenceLength": "1000", "470 AO-Name": "AnalogIO", "XLIGHT Disk slider-Label": "Disk Pos 40um", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "Binning": "1x1", "TE2000-ModelType": "1", "COM3-AnswerTimeout": "500.0000", "555 Shutter-SupportsTriggering": "0", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "TE2000-Port": "COM4", "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Description": "NI DAC", "COM4-AnswerTimeout": "500.0000", "COM1-DelayBetweenCharsMs": "0.0000", "FilterBlock-Label": "Confocal", "445 AO-Name": "AnalogIO", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-Timing-ReadoutTimeNs": "24672000.0000", "528 AO-Demo": "No", "520 AO-Sequenceable": "1", "555-Description": "DA device that is used as a shutter", "405-DA Device": "405 Shutter", "405 AO-TriggerSequenceLength": "1000", "640 AO-MinVolts": "0.0000", "640 Shutter-Block voltage": "false", "FrameIndex": 0, "PRIME-Port": "CMOS", "555 AO-MinVolts": "0.0000", "COM3-Description": "Serial port driver (boost:asio)", "528 AO-Description": "NI DAC", "555 AO-IOChannel": "Dev1/ao6", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "555 AO-TriggerInputLine": "/Dev1/PFI0", "PRIME-ActualInterval-ms": "374.3700", "LudlController-Port": "COM9", "445 Shutter-Demo": "No", "PRIME-Trigger-Expose Out-Mux": "1", "SliceIndex": 2, "PRIME-CCDTemperatureSetPoint": "-15.0000", "405 Shutter-MinVolts": "0.0000", "PRIME-ExposeOutMode": "First Row", "AxisPositions": null, "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Volts": "0.0000", "528-Name": "DA Shutter", "555-State": "0", "XLIGHT Dichroic-Label": "Di03-R635-t1", "445 AO-Sequenceable": "1", "405-Name": "DA Shutter", "405 AO-IOChannel": "Dev1/ao0", "Core-Camera": "PRIME", "Focus-Name": "Focus", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "405 Shutter-IOChannel": "Dev1/ao8", "640 AO-Demo": "No", "640 Shutter-SupportsTriggering": "0", "445 AO-Demo": "No", "555-DA Device": "555 Shutter", "470 Shutter-Description": "NI DAC", "528 AO-Volts": "0.0000", "COM1-DataBits": "8", "Vincent-D3-Address": "x", "520 AO-SupportsTriggering": "0", "405-Description": "DA device that is used as a shutter", "640 Shutter-MinVolts": "0.0000", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "405 AO-Name": "AnalogIO", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "640 AO-TriggerInputLine": "/Dev1/PFI0", "405 AO-TriggerInputLine": "/Dev1/PFI0", "640-Description": "DA device that is used as a shutter", "445 Shutter-TriggerSampleRate": "100000", "470 Shutter-TriggerSequenceLength": "1000", "COM1-StopBits": "1", "520 Shutter-SupportsTriggering": "0", "405-State": "0", "Core-Initialize": "1", "XLIGHT Emission Wheel-State": "7", "520 AO-Description": "NI DAC", "528 AO-TriggerInputLine": "/Dev1/PFI0", "445 Shutter-MaxVolts": "5.0000", "COM9-Handshaking": "Off", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "XLIGHT Spin motor-Label": "On (spinning)", "PRIME-PP 5 ENABLED": "No", "UUID": "fbd958bc-e7da-4bb6-97b1-a01bf3d9155d", "PRIME-TriggerMode": "Internal Trigger", "COM1-Handshaking": "Off", "PRIME-Timing-PostTriggerDelayNs": "49344000.0000", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "Focus-Position": "16479.8000", "528 Shutter-Block voltage": "false", "Channel": "Il-C", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "555 Shutter-Demo": "No", "405 Shutter-Demo": "No", "528 AO-Name": "AnalogIO", "520-State": "0", "Core-Galvo": "", "405 Shutter-TriggerSampleRate": "100000", "Focus-FocusStepSize_(nm)": "100", "PRIME-PixelType": "16bit", "COM4-DataBits": "8", "470 AO-IOChannel": "Dev1/ao2", "520 AO-MinVolts": "0.0000", "COM9-Parity": "None", "PRIME-CircularBufferEnabled": "ON", "PRIME-PP 6 Reset": "No", "640 AO-Description": "NI DAC", "Source": "PRIME", "445 AO-MinVolts": "0.0000", "PIEZO-Name": "Stage", "445 AO-Description": "NI DAC", "PRIME-CircularBufferFrameCount": "46", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "520 AO-Volts": "0.0000", "528 Shutter-TriggerSampleRate": "100000", "XPositionUm": 62321, "OpticalPath-State": "4", "PRIME-PP 3 THRESHOLD": "80", "PRIME-PVCAM Version": "3.7.1", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Gain": "1-HDR", "640 Shutter-TriggerSequenceLength": "1000", "528 AO-SupportsTriggering": "0", "445 AO-TriggerInputLine": "/Dev1/PFI0", "OpticalPath-Name": "OpticalPath", "PRIME-PP 4 ENABLED": "Yes", "PRIME-PVCAM Adapter Version": "1.3.29", "COM3-Name": "COM3", "405 AO-MaxVolts": "5.0000", "520 Shutter-Block voltage": "false", "PRIME-Timing-ClearingTimeNs": "49344000.0000", "Core-Focus": "PIEZO", "528 AO-Block voltage": "false", "TE2000-Description": "Nikon TE2000 hub", "528 AO-MinVolts": "0.0000", "COM4-Parity": "None", "Summary": { "Slices": 11, "ChContrastMin": [ 0, 0, 0, 0, 0, 0 ], "PixelAspect": 1, "SlicesFirst": true, "Interval_ms": 0, "Time": "2019-12-17 21:58:39 -0500", "Prefix": "seq_3", "InitialPositionList": [ { "Label": "Pos0", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.435], "Focus": [16480.2], "XYStage": [ 69822.40000000001, 20100.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos1", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.425], "Focus": [16480.3], "XYStage": [ 69004, 20101 ] }, "GridColumnIndex": 0 }, { "Label": "Pos2", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.44], "Focus": [16480.1], "XYStage": [ 63119.5, 20690.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos3", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.405], "Focus": [16480.2], "XYStage": [ 62321, 20690.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos4", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.415], "Focus": [16480], "XYStage": [ 56604.100000000006, 20688.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos5", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.42000000000002], "Focus": [16480.1], "XYStage": [ 56034.200000000004, 20688.800000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos6", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.455], "Focus": [16480], "XYStage": [ 50889.3, 20496.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos7", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.43], "Focus": [16480.2], "XYStage": [ 50101.600000000006, 20496.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos8", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.67000000000002], "Focus": [16479.9], "XYStage": [ 44818.600000000006, 20496.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos9", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.67000000000002], "Focus": [16480], "XYStage": [ 43897.9, 20436.800000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos10", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.675], "Focus": [16479.9], "XYStage": [ 39670.600000000006, 20664.9 ] }, "GridColumnIndex": 0 }, { "Label": "Pos11", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.69], "Focus": [16479.9], "XYStage": [ 38734, 20664.600000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos12", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.695], "Focus": [16480.1], "XYStage": [ 34037.200000000004, 20664.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos13", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.685], "Focus": [16480.1], "XYStage": [ 33152, 20664.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos14", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.69], "Focus": [16480.1], "XYStage": [ 28122.800000000003, 20664.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos15", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.675], "Focus": [16480], "XYStage": [ 27422.9, 20664.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos16", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.64000000000001], "Focus": [16480], "XYStage": [ 23005.2, 20664.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos17", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.69], "Focus": [16479.9], "XYStage": [ 21820.600000000002, 20409.600000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos18", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.41], "Focus": [16480.1], "XYStage": [ 73697.6, -13222.800000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos19", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.455], "Focus": [16480.1], "XYStage": [ 72765.8, -13223 ] }, "GridColumnIndex": 0 }, { "Label": "Pos20", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.4], "Focus": [16480.2], "XYStage": [ 67445.5, -12303.800000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos21", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.415], "Focus": [16480.2], "XYStage": [ 66613.3, -12303.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos22", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.415], "Focus": [16480.1], "XYStage": [ 61819.4, -11936.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos23", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.405], "Focus": [16480.2], "XYStage": [ 61325.700000000004, -11937.1 ] }, "GridColumnIndex": 0 }, { "Label": "Pos24", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.46], "Focus": [16480.2], "XYStage": [ 55377, -10974.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos25", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.445], "Focus": [16480.1], "XYStage": [ 54827, -11214.800000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos26", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.435], "Focus": [16480.2], "XYStage": [ 49937.100000000006, -10919.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos27", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.425], "Focus": [16480.1], "XYStage": [ 48764, -10919.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos28", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.42000000000002], "Focus": [16480.2], "XYStage": [ 43978.4, -10920.400000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos29", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.435], "Focus": [16480.2], "XYStage": [ 43106.3, -10920.400000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos30", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.405], "Focus": [16480.3], "XYStage": [ 38212.4, -10920.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos31", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.445], "Focus": [16480.2], "XYStage": [ 37254.9, -10920.400000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos32", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.44], "Focus": [16480.3], "XYStage": [ 32472.800000000003, -11294.400000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos33", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [166.95000000000002], "Focus": [16480.2], "XYStage": [ 31420.7, -11294.400000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos34", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [166.95000000000002], "Focus": [16480.2], "XYStage": [ 26881.5, -11640 ] }, "GridColumnIndex": 0 }, { "Label": "Pos35", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [166.98], "Focus": [16480.2], "XYStage": [ 25876.100000000002, -11641.1 ] }, "GridColumnIndex": 0 } ], "z-step_um": 10, "GridRow": 0, "BitDepth": 16, "Depth": 2, "Channels": 6, "Source": "Micro-Manager", "ChColors": [ -16711681, -256, -65281, -1, -1, -16776961 ], "MetadataVersion": 10, "KeepShutterOpenChannels": true, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536, 65536 ], "CustomIntervals_ms": [], "Height": 1200, "MicroManagerVersion": "1.4.23 20180219", "GridColumn": 0, "UUID": "8c8f9d20-b128-4577-9f91-89e70f22d319", "PixelType": "GRAY16", "Width": 1200, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\Yan\\20191217-YC113-S5S6", "Frames": 1, "Comment": "", "UserName": "Nikon", "PixelSize_um": 1.1, "ROI": [ 0, 0, 1200, 1200 ], "Date": "2019-12-17", "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC", "P-DAPI-high" ], "KeepShutterOpenSlices": true, "IJType": 1, "ComputerName": "ZadorConfocal2", "Positions": 36, "PositionIndex": 3, "CameraTimeout": 20000, "TimeFirst": false }, "640 AO-Name": "AnalogIO", "Vincent-D3-Command": "Close" }, "FrameKey-0-3-3": { "COM4-StopBits": "1", "528 AO-MaxVolts": "5.0000", "XLIGHT Excitation Wheel-State": "2", "470 Shutter-Name": "AnalogIO", "Core-Shutter": "640", "555 AO-Demo": "No", "XLIGHT Dichroic-State": "2", "XYStage-StepSize-Y": "0.1000", "ZPositionUm": 157.405, "XYStage-StepSize-X": "0.1000", "PRIME-FirmwareVersion": "20.28", "PRIME-ClearCycles": "2", "520-Name": "DA Shutter", "405 Shutter-Name": "AnalogIO", "PIEZO-StepSize": "0.0050", "PRIME-ClearMode": "Pre-Exposure", "470 AO-MinVolts": "0.0000", "405 AO-SupportsTriggering": "1", "CameraChannelIndex": 0, "XYStage-StartSpeed": "500.0000", "COM9-StopBits": "2", "405 AO-Description": "NI DAC", "COM4-DelayBetweenCharsMs": "0.0000", "445 AO-Block voltage": "false", "520 AO-TriggerInputLine": "/Dev1/PFI0", "555 AO-Description": "NI DAC", "520 AO-TriggerSampleRate": "100000", "COM4-Verbose": "1", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM4-Name": "COM4", "405 Shutter-Volts": "0.0000", "Vincent-D3-Port": "COM1", "PRIME-PP 4 MIN ADU AFFECTED": "200", "470 Shutter-MinVolts": "0.0000", "PRIME-PP 3 ENABLED": "Yes", "470 AO-Description": "NI DAC", "PRIME-Description": "PVCAM API device adapter", "520 Shutter-MaxVolts": "5.0000", "520 Shutter-Volts": "0.0000", "528 AO-Sequenceable": "1", "PRIME-Binning": "1x1", "555 Shutter-MaxVolts": "5.0000", "528 Shutter-TriggerSequenceLength": "1000", "405 Shutter-Block voltage": "false", "XLIGHT Spin motor-State": "1", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "Vincent-D3-Time to open (ms)": "35.0000", "Vincent-D3-Time to close (ms)": "35.0000", "445 AO-TriggerSampleRate": "100000", "640 Shutter-IOChannel": "Dev1/ao14", "555 AO-Sequenceable": "1", "520 AO-Name": "AnalogIO", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "470 AO-Block voltage": "false", "COM1-Verbose": "1", "COM9-AnswerTimeout": "10000.0000", "528 Shutter-Name": "AnalogIO", "PRIME-Trigger Timeout (secs)": "10", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "NextFrame": 0, "470 AO-TriggerSequenceLength": "1000", "PRIME-TransposeXY": "0", "445 AO-TriggerSequenceLength": "1000", "COM1-Name": "COM1", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "COM9-DataBits": "8", "COM1-AnswerTimeout": "500.0000", "Camera": "", "TE2000-Version": "V2.50-V2.50-V2.10", "528 Shutter-Volts": "0.0000", "XLIGHT Hub-Name": "XLIGHT Hub", "PIEZO-Description": "Ludl stage driver adapter", "PositionName": "Pos3", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSampleRate": "100000", "PRIME-FanSpeedSetpoint": "High", "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "405 AO-TriggerSampleRate": "100000", "470 Shutter-Block voltage": "false", "470-State": "0", "COM3-Parity": "None", "405 AO-Volts": "0.0000", "PRIME-TransposeMirrorY": "0", "PRIME-ChipName": "GS144BSI", "470 AO-Volts": "0.0000", "PRIME-TransposeMirrorX": "0", "445 Shutter-SupportsTriggering": "0", "470 Shutter-SupportsTriggering": "0", "XLIGHT Hub-Description": "XLIGHT hub", "PRIME-PreampOffLimit": "10000", "470 AO-Sequenceable": "1", "528 Shutter-MaxVolts": "5.0000", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "Core-XYStage": "XYStage", "470 AO-TriggerSampleRate": "100000", "PRIME-Timing-PreTriggerDelayNs": "24672000.0000", "528-DA Device": "528 Shutter", "Focus-Description": "Nikon TE2000 focus adapter", "FilterBlock-State": "5", "520 AO-Demo": "No", "COM3-DelayBetweenCharsMs": "0.0000", "470 Shutter-Volts": "0.0000", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "PRIME-PP 2 THRESHOLD": "125", "PRIME-PP 4 THRESHOLD": "75", "XYStage-Acceleration": "4.0000", "470 Shutter-Demo": "No", "PRIME-Name": "PMPCIECam00", "640-DA Device": "640 Shutter", "405 AO-Block voltage": "false", "ElapsedTime-ms": 153586, "PRIME-Offset": "100", "445 AO-IOChannel": "Dev1/ao1", "640 AO-MaxVolts": "5.0000", "PositionIndex": 3, "PRIME-PP 1 ENABLED": "Yes", "COM1-BaudRate": "9600", "640-State": "1", "COM3-Verbose": "1", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "LudlController-TransmissionDelay": "0", "PRIME-FullWellCapacity": "62000", "PRIME-SMARTStreamingEnabled": "No", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "640 Shutter-Description": "NI DAC", "528-Description": "DA device that is used as a shutter", "COM4-Description": "Serial port driver (boost:asio)", "405 Shutter-Description": "NI DAC", "TE2000-Name": "TE2000", "528 Shutter-Description": "NI DAC", "COM9-Description": "Serial port driver (boost:asio)", "LudlController-Name": "LudlController", "445 Shutter-TriggerSequenceLength": "1000", "PRIME-SerialNumber": "A18B203008", "445 AO-Volts": "0.0000", "PRIME-X-dimension": "1200", "PRIME-ShutterCloseDelay": "0", "XLIGHT Disk slider-State": "2", "Position": "Default", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 AO-IOChannel": "Dev1/ao5", "520 AO-IOChannel": "Dev1/ao4", "528 AO-TriggerSequenceLength": "1000", "405 Shutter-MaxVolts": "5.0000", "470-DA Device": "470 Shutter", "PRIME-FTCapable": "No", "Core-ChannelGroup": "Channels", "555 AO-SupportsTriggering": "0", "PRIME-Y-dimension": "1200", "Frame": 0, "555 Shutter-Sequenceable": "1", "PRIME-Timing-ExposureTimeNs": "9992000.0000", "Core-ImageProcessor": "", "528-State": "0", "520 AO-Block voltage": "false", "XLIGHT Excitation Wheel-Label": "QUAD", "640 Shutter-Demo": "No", "COM3-StopBits": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "520 Shutter-IOChannel": "Dev1/ao11", "Width": 1200, "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-SupportsTriggering": "0", "640 AO-TriggerSampleRate": "100000", "520-Description": "DA device that is used as a shutter", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "405 Shutter-Sequenceable": "1", "405 Shutter-SupportsTriggering": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-TriggerSampleRate": "100000", "640 Shutter-MaxVolts": "5.0000", "555 AO-MaxVolts": "5.0000", "520 AO-TriggerSequenceLength": "1000", "COM3-BaudRate": "9600", "COM9-BaudRate": "9600", "Vincent-D3-Channel #": "Ch. #1", "PRIME-PMode": "Normal", "OpticalPath-Label": "Left(100)", "BitDepth": 16, "555 AO-TriggerSequenceLength": "1000", "445 Shutter-Description": "NI DAC", "405 AO-Sequenceable": "1", "PRIME-AcquisitionMethod": "Callbacks", "555 AO-Name": "AnalogIO", "PRIME-ShutterMode": "Pre-Exposure", "COM3-Handshaking": "Off", "445 AO-SupportsTriggering": "0", "ROI": "0-0-1200-1200", "COM9-Verbose": "1", "PRIME-Color": "OFF", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "PRIME-ShutterOpenDelay": "0", "COM1-Parity": "None", "Slice": 3, "SlicePosition": -20, "COM9-DelayBetweenCharsMs": "0.0000", "555-Name": "DA Shutter", "PIEZO-LudlSingleAxisName": "B", "XLIGHT Emission Wheel-Label": "725/40 (C)", "640 Shutter-Name": "AnalogIO", "640 AO-Sequenceable": "1", "520 Shutter-Demo": "No", "528 Shutter-Demo": "No", "470-Name": "DA Shutter", "520-DA Device": "520 Shutter", "XYStage-Description": "Ludl XY stage driver adapter", "445 Shutter-MinVolts": "0.0000", "528 AO-TriggerSampleRate": "100000", "405 AO-MinVolts": "0.0000", "405 Shutter-TriggerSequenceLength": "1000", "Height": 1200, "555 AO-Block voltage": "false", "640 Shutter-Volts": "5.0000", "PixelType": "GRAY16", "445 Shutter-Block voltage": "false", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "520 Shutter-TriggerSequenceLength": "1000", "COM9-Name": "COM9", "Core-SLM": "", "555 AO-Volts": "0.0000", "555 Shutter-Block voltage": "false", "XYStage-Speed": "1800.0000", "520 AO-MaxVolts": "5.0000", "528 Shutter-SupportsTriggering": "0", "COM1-Description": "Serial port driver (boost:asio)", "PRIME-MetadataEnabled": "No", "520 Shutter-MinVolts": "0.0000", "470 Shutter-Sequenceable": "1", "PRIME-PreampDelay": "0", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-MinVolts": "0.0000", "ChannelIndex": 3, "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Core-AutoShutter": "1", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "Exposure-ms": 520, "PRIME-PP 2 ENABLED": "Yes", "640 Shutter-TriggerSampleRate": "100000", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "470 Shutter-IOChannel": "Dev1/ao10", "405 AO-Demo": "No", "PixelSizeUm": 1.1, "470 AO-Demo": "No", "470 Shutter-MaxVolts": "5.0000", "Vincent-D3-Name": "Vincent-D3", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FileName": "img_000000000_Il-C_003.tif", "LudlController-Reset": "Operate", "PRIME-Exposure": "520.00", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "445-Name": "DA Shutter", "555 Shutter-Name": "AnalogIO", "520 Shutter-TriggerSampleRate": "100000", "445 Shutter-IOChannel": "Dev1/ao9", "PRIME-TransposeCorrection": "0", "XYStage-StepSize": "0.1000", "445-Description": "DA device that is used as a shutter", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 AO-IOChannel": "Dev1/ao7", "520 Shutter-Name": "AnalogIO", "COM3-DataBits": "8", "640 AO-Volts": "4.5000", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "555 Shutter-IOChannel": "Dev1/ao13", "WaitInterval": null, "520 Shutter-Sequenceable": "1", "YPositionUm": 20690.300000000003, "445-State": "0", "445-DA Device": "445 Shutter", "PRIME-CircularBufferAutoSize": "ON", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "555 AO-TriggerSampleRate": "100000", "445 Shutter-Volts": "0.0000", "XLIGHT Hub-Port": "COM3", "Core-AutoFocus": "", "COM4-Handshaking": "Off", "528 Shutter-MinVolts": "0.0000", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "445 Shutter-Name": "AnalogIO", "528 Shutter-Sequenceable": "1", "XYStage-TransposeMirrorX": "0", "XYStage-TransposeMirrorY": "0", "PRIME-ReadoutRate": "100MHz 16bit", "XYStage-Name": "Stage", "640 AO-SupportsTriggering": "0", "640-Name": "DA Shutter", "640 Shutter-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "640 AO-Block voltage": "false", "445 Shutter-Sequenceable": "1", "470 AO-MaxVolts": "5.0000", "COM4-BaudRate": "9600", "470-Description": "DA device that is used as a shutter", "520 Shutter-Description": "NI DAC", "PRIME-CCDTemperature": "-14.9600", "Time": "2019-12-17 21:59:01 -0500", "640 AO-TriggerSequenceLength": "1000", "470 AO-Name": "AnalogIO", "XLIGHT Disk slider-Label": "Disk Pos 40um", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "Binning": "1x1", "TE2000-ModelType": "1", "COM3-AnswerTimeout": "500.0000", "555 Shutter-SupportsTriggering": "0", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "TE2000-Port": "COM4", "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Description": "NI DAC", "COM4-AnswerTimeout": "500.0000", "COM1-DelayBetweenCharsMs": "0.0000", "FilterBlock-Label": "Confocal", "445 AO-Name": "AnalogIO", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-Timing-ReadoutTimeNs": "24672000.0000", "528 AO-Demo": "No", "520 AO-Sequenceable": "1", "555-Description": "DA device that is used as a shutter", "405-DA Device": "405 Shutter", "405 AO-TriggerSequenceLength": "1000", "640 AO-MinVolts": "0.0000", "640 Shutter-Block voltage": "false", "FrameIndex": 0, "PRIME-Port": "CMOS", "555 AO-MinVolts": "0.0000", "COM3-Description": "Serial port driver (boost:asio)", "528 AO-Description": "NI DAC", "555 AO-IOChannel": "Dev1/ao6", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "555 AO-TriggerInputLine": "/Dev1/PFI0", "PRIME-ActualInterval-ms": "374.3700", "LudlController-Port": "COM9", "445 Shutter-Demo": "No", "PRIME-Trigger-Expose Out-Mux": "1", "SliceIndex": 3, "PRIME-CCDTemperatureSetPoint": "-15.0000", "405 Shutter-MinVolts": "0.0000", "PRIME-ExposeOutMode": "First Row", "AxisPositions": null, "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Volts": "0.0000", "528-Name": "DA Shutter", "555-State": "0", "XLIGHT Dichroic-Label": "Di03-R635-t1", "445 AO-Sequenceable": "1", "405-Name": "DA Shutter", "405 AO-IOChannel": "Dev1/ao0", "Core-Camera": "PRIME", "Focus-Name": "Focus", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "405 Shutter-IOChannel": "Dev1/ao8", "640 AO-Demo": "No", "640 Shutter-SupportsTriggering": "0", "445 AO-Demo": "No", "555-DA Device": "555 Shutter", "470 Shutter-Description": "NI DAC", "528 AO-Volts": "0.0000", "COM1-DataBits": "8", "Vincent-D3-Address": "x", "520 AO-SupportsTriggering": "0", "405-Description": "DA device that is used as a shutter", "640 Shutter-MinVolts": "0.0000", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "405 AO-Name": "AnalogIO", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "640 AO-TriggerInputLine": "/Dev1/PFI0", "405 AO-TriggerInputLine": "/Dev1/PFI0", "640-Description": "DA device that is used as a shutter", "445 Shutter-TriggerSampleRate": "100000", "470 Shutter-TriggerSequenceLength": "1000", "COM1-StopBits": "1", "520 Shutter-SupportsTriggering": "0", "405-State": "0", "Core-Initialize": "1", "XLIGHT Emission Wheel-State": "7", "520 AO-Description": "NI DAC", "528 AO-TriggerInputLine": "/Dev1/PFI0", "445 Shutter-MaxVolts": "5.0000", "COM9-Handshaking": "Off", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "XLIGHT Spin motor-Label": "On (spinning)", "PRIME-PP 5 ENABLED": "No", "UUID": "81e5681b-9617-44f4-b88f-ba93b9cb214c", "PRIME-TriggerMode": "Internal Trigger", "COM1-Handshaking": "Off", "PRIME-Timing-PostTriggerDelayNs": "49344000.0000", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "Focus-Position": "16479.8000", "528 Shutter-Block voltage": "false", "Channel": "Il-C", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "555 Shutter-Demo": "No", "405 Shutter-Demo": "No", "528 AO-Name": "AnalogIO", "520-State": "0", "Core-Galvo": "", "405 Shutter-TriggerSampleRate": "100000", "Focus-FocusStepSize_(nm)": "100", "PRIME-PixelType": "16bit", "COM4-DataBits": "8", "470 AO-IOChannel": "Dev1/ao2", "520 AO-MinVolts": "0.0000", "COM9-Parity": "None", "PRIME-CircularBufferEnabled": "ON", "PRIME-PP 6 Reset": "No", "640 AO-Description": "NI DAC", "Source": "PRIME", "445 AO-MinVolts": "0.0000", "PIEZO-Name": "Stage", "445 AO-Description": "NI DAC", "PRIME-CircularBufferFrameCount": "46", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "520 AO-Volts": "0.0000", "528 Shutter-TriggerSampleRate": "100000", "XPositionUm": 62321, "OpticalPath-State": "4", "PRIME-PP 3 THRESHOLD": "80", "PRIME-PVCAM Version": "3.7.1", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Gain": "1-HDR", "640 Shutter-TriggerSequenceLength": "1000", "528 AO-SupportsTriggering": "0", "445 AO-TriggerInputLine": "/Dev1/PFI0", "OpticalPath-Name": "OpticalPath", "PRIME-PP 4 ENABLED": "Yes", "PRIME-PVCAM Adapter Version": "1.3.29", "COM3-Name": "COM3", "405 AO-MaxVolts": "5.0000", "520 Shutter-Block voltage": "false", "PRIME-Timing-ClearingTimeNs": "49344000.0000", "Core-Focus": "PIEZO", "528 AO-Block voltage": "false", "TE2000-Description": "Nikon TE2000 hub", "528 AO-MinVolts": "0.0000", "COM4-Parity": "None", "Summary": { "Slices": 11, "ChContrastMin": [ 0, 0, 0, 0, 0, 0 ], "PixelAspect": 1, "SlicesFirst": true, "Interval_ms": 0, "Time": "2019-12-17 21:58:39 -0500", "Prefix": "seq_3", "InitialPositionList": [ { "Label": "Pos0", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.435], "Focus": [16480.2], "XYStage": [ 69822.40000000001, 20100.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos1", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.425], "Focus": [16480.3], "XYStage": [ 69004, 20101 ] }, "GridColumnIndex": 0 }, { "Label": "Pos2", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.44], "Focus": [16480.1], "XYStage": [ 63119.5, 20690.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos3", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.405], "Focus": [16480.2], "XYStage": [ 62321, 20690.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos4", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.415], "Focus": [16480], "XYStage": [ 56604.100000000006, 20688.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos5", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.42000000000002], "Focus": [16480.1], "XYStage": [ 56034.200000000004, 20688.800000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos6", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.455], "Focus": [16480], "XYStage": [ 50889.3, 20496.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos7", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.43], "Focus": [16480.2], "XYStage": [ 50101.600000000006, 20496.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos8", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.67000000000002], "Focus": [16479.9], "XYStage": [ 44818.600000000006, 20496.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos9", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.67000000000002], "Focus": [16480], "XYStage": [ 43897.9, 20436.800000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos10", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.675], "Focus": [16479.9], "XYStage": [ 39670.600000000006, 20664.9 ] }, "GridColumnIndex": 0 }, { "Label": "Pos11", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.69], "Focus": [16479.9], "XYStage": [ 38734, 20664.600000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos12", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.695], "Focus": [16480.1], "XYStage": [ 34037.200000000004, 20664.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos13", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.685], "Focus": [16480.1], "XYStage": [ 33152, 20664.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos14", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.69], "Focus": [16480.1], "XYStage": [ 28122.800000000003, 20664.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos15", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.675], "Focus": [16480], "XYStage": [ 27422.9, 20664.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos16", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.64000000000001], "Focus": [16480], "XYStage": [ 23005.2, 20664.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos17", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.69], "Focus": [16479.9], "XYStage": [ 21820.600000000002, 20409.600000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos18", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.41], "Focus": [16480.1], "XYStage": [ 73697.6, -13222.800000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos19", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.455], "Focus": [16480.1], "XYStage": [ 72765.8, -13223 ] }, "GridColumnIndex": 0 }, { "Label": "Pos20", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.4], "Focus": [16480.2], "XYStage": [ 67445.5, -12303.800000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos21", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.415], "Focus": [16480.2], "XYStage": [ 66613.3, -12303.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos22", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.415], "Focus": [16480.1], "XYStage": [ 61819.4, -11936.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos23", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.405], "Focus": [16480.2], "XYStage": [ 61325.700000000004, -11937.1 ] }, "GridColumnIndex": 0 }, { "Label": "Pos24", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.46], "Focus": [16480.2], "XYStage": [ 55377, -10974.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos25", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.445], "Focus": [16480.1], "XYStage": [ 54827, -11214.800000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos26", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.435], "Focus": [16480.2], "XYStage": [ 49937.100000000006, -10919.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos27", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.425], "Focus": [16480.1], "XYStage": [ 48764, -10919.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos28", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.42000000000002], "Focus": [16480.2], "XYStage": [ 43978.4, -10920.400000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos29", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.435], "Focus": [16480.2], "XYStage": [ 43106.3, -10920.400000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos30", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.405], "Focus": [16480.3], "XYStage": [ 38212.4, -10920.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos31", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.445], "Focus": [16480.2], "XYStage": [ 37254.9, -10920.400000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos32", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.44], "Focus": [16480.3], "XYStage": [ 32472.800000000003, -11294.400000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos33", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [166.95000000000002], "Focus": [16480.2], "XYStage": [ 31420.7, -11294.400000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos34", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [166.95000000000002], "Focus": [16480.2], "XYStage": [ 26881.5, -11640 ] }, "GridColumnIndex": 0 }, { "Label": "Pos35", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [166.98], "Focus": [16480.2], "XYStage": [ 25876.100000000002, -11641.1 ] }, "GridColumnIndex": 0 } ], "z-step_um": 10, "GridRow": 0, "BitDepth": 16, "Depth": 2, "Channels": 6, "Source": "Micro-Manager", "ChColors": [ -16711681, -256, -65281, -1, -1, -16776961 ], "MetadataVersion": 10, "KeepShutterOpenChannels": true, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536, 65536 ], "CustomIntervals_ms": [], "Height": 1200, "MicroManagerVersion": "1.4.23 20180219", "GridColumn": 0, "UUID": "8c8f9d20-b128-4577-9f91-89e70f22d319", "PixelType": "GRAY16", "Width": 1200, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\Yan\\20191217-YC113-S5S6", "Frames": 1, "Comment": "", "UserName": "Nikon", "PixelSize_um": 1.1, "ROI": [ 0, 0, 1200, 1200 ], "Date": "2019-12-17", "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC", "P-DAPI-high" ], "KeepShutterOpenSlices": true, "IJType": 1, "ComputerName": "ZadorConfocal2", "Positions": 36, "PositionIndex": 3, "CameraTimeout": 20000, "TimeFirst": false }, "640 AO-Name": "AnalogIO", "Vincent-D3-Command": "Close" }, "FrameKey-0-3-4": { "COM4-StopBits": "1", "528 AO-MaxVolts": "5.0000", "XLIGHT Excitation Wheel-State": "2", "470 Shutter-Name": "AnalogIO", "Core-Shutter": "640", "555 AO-Demo": "No", "XLIGHT Dichroic-State": "2", "XYStage-StepSize-Y": "0.1000", "ZPositionUm": 167.405, "XYStage-StepSize-X": "0.1000", "PRIME-FirmwareVersion": "20.28", "PRIME-ClearCycles": "2", "520-Name": "DA Shutter", "405 Shutter-Name": "AnalogIO", "PIEZO-StepSize": "0.0050", "PRIME-ClearMode": "Pre-Exposure", "470 AO-MinVolts": "0.0000", "405 AO-SupportsTriggering": "1", "CameraChannelIndex": 0, "XYStage-StartSpeed": "500.0000", "COM9-StopBits": "2", "405 AO-Description": "NI DAC", "COM4-DelayBetweenCharsMs": "0.0000", "445 AO-Block voltage": "false", "520 AO-TriggerInputLine": "/Dev1/PFI0", "555 AO-Description": "NI DAC", "520 AO-TriggerSampleRate": "100000", "COM4-Verbose": "1", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM4-Name": "COM4", "405 Shutter-Volts": "0.0000", "Vincent-D3-Port": "COM1", "PRIME-PP 4 MIN ADU AFFECTED": "200", "470 Shutter-MinVolts": "0.0000", "PRIME-PP 3 ENABLED": "Yes", "470 AO-Description": "NI DAC", "PRIME-Description": "PVCAM API device adapter", "520 Shutter-MaxVolts": "5.0000", "520 Shutter-Volts": "0.0000", "528 AO-Sequenceable": "1", "PRIME-Binning": "1x1", "555 Shutter-MaxVolts": "5.0000", "528 Shutter-TriggerSequenceLength": "1000", "405 Shutter-Block voltage": "false", "XLIGHT Spin motor-State": "1", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "Vincent-D3-Time to open (ms)": "35.0000", "Vincent-D3-Time to close (ms)": "35.0000", "445 AO-TriggerSampleRate": "100000", "640 Shutter-IOChannel": "Dev1/ao14", "555 AO-Sequenceable": "1", "520 AO-Name": "AnalogIO", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "470 AO-Block voltage": "false", "COM1-Verbose": "1", "COM9-AnswerTimeout": "10000.0000", "528 Shutter-Name": "AnalogIO", "PRIME-Trigger Timeout (secs)": "10", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "NextFrame": 0, "470 AO-TriggerSequenceLength": "1000", "PRIME-TransposeXY": "0", "445 AO-TriggerSequenceLength": "1000", "COM1-Name": "COM1", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "COM9-DataBits": "8", "COM1-AnswerTimeout": "500.0000", "Camera": "", "TE2000-Version": "V2.50-V2.50-V2.10", "528 Shutter-Volts": "0.0000", "XLIGHT Hub-Name": "XLIGHT Hub", "PIEZO-Description": "Ludl stage driver adapter", "PositionName": "Pos3", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSampleRate": "100000", "PRIME-FanSpeedSetpoint": "High", "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "405 AO-TriggerSampleRate": "100000", "470 Shutter-Block voltage": "false", "470-State": "0", "COM3-Parity": "None", "405 AO-Volts": "0.0000", "PRIME-TransposeMirrorY": "0", "PRIME-ChipName": "GS144BSI", "470 AO-Volts": "0.0000", "PRIME-TransposeMirrorX": "0", "445 Shutter-SupportsTriggering": "0", "470 Shutter-SupportsTriggering": "0", "XLIGHT Hub-Description": "XLIGHT hub", "PRIME-PreampOffLimit": "10000", "470 AO-Sequenceable": "1", "528 Shutter-MaxVolts": "5.0000", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "Core-XYStage": "XYStage", "470 AO-TriggerSampleRate": "100000", "PRIME-Timing-PreTriggerDelayNs": "24672000.0000", "528-DA Device": "528 Shutter", "Focus-Description": "Nikon TE2000 focus adapter", "FilterBlock-State": "5", "520 AO-Demo": "No", "COM3-DelayBetweenCharsMs": "0.0000", "470 Shutter-Volts": "0.0000", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "PRIME-PP 2 THRESHOLD": "125", "PRIME-PP 4 THRESHOLD": "75", "XYStage-Acceleration": "4.0000", "470 Shutter-Demo": "No", "PRIME-Name": "PMPCIECam00", "640-DA Device": "640 Shutter", "405 AO-Block voltage": "false", "ElapsedTime-ms": 154357, "PRIME-Offset": "100", "445 AO-IOChannel": "Dev1/ao1", "640 AO-MaxVolts": "5.0000", "PositionIndex": 3, "PRIME-PP 1 ENABLED": "Yes", "COM1-BaudRate": "9600", "640-State": "1", "COM3-Verbose": "1", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "LudlController-TransmissionDelay": "0", "PRIME-FullWellCapacity": "62000", "PRIME-SMARTStreamingEnabled": "No", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "640 Shutter-Description": "NI DAC", "528-Description": "DA device that is used as a shutter", "COM4-Description": "Serial port driver (boost:asio)", "405 Shutter-Description": "NI DAC", "TE2000-Name": "TE2000", "528 Shutter-Description": "NI DAC", "COM9-Description": "Serial port driver (boost:asio)", "LudlController-Name": "LudlController", "445 Shutter-TriggerSequenceLength": "1000", "PRIME-SerialNumber": "A18B203008", "445 AO-Volts": "0.0000", "PRIME-X-dimension": "1200", "PRIME-ShutterCloseDelay": "0", "XLIGHT Disk slider-State": "2", "Position": "Default", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 AO-IOChannel": "Dev1/ao5", "520 AO-IOChannel": "Dev1/ao4", "528 AO-TriggerSequenceLength": "1000", "405 Shutter-MaxVolts": "5.0000", "470-DA Device": "470 Shutter", "PRIME-FTCapable": "No", "Core-ChannelGroup": "Channels", "555 AO-SupportsTriggering": "0", "PRIME-Y-dimension": "1200", "Frame": 0, "555 Shutter-Sequenceable": "1", "PRIME-Timing-ExposureTimeNs": "9992000.0000", "Core-ImageProcessor": "", "528-State": "0", "520 AO-Block voltage": "false", "XLIGHT Excitation Wheel-Label": "QUAD", "640 Shutter-Demo": "No", "COM3-StopBits": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "520 Shutter-IOChannel": "Dev1/ao11", "Width": 1200, "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-SupportsTriggering": "0", "640 AO-TriggerSampleRate": "100000", "520-Description": "DA device that is used as a shutter", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "405 Shutter-Sequenceable": "1", "405 Shutter-SupportsTriggering": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-TriggerSampleRate": "100000", "640 Shutter-MaxVolts": "5.0000", "555 AO-MaxVolts": "5.0000", "520 AO-TriggerSequenceLength": "1000", "COM3-BaudRate": "9600", "COM9-BaudRate": "9600", "Vincent-D3-Channel #": "Ch. #1", "PRIME-PMode": "Normal", "OpticalPath-Label": "Left(100)", "BitDepth": 16, "555 AO-TriggerSequenceLength": "1000", "445 Shutter-Description": "NI DAC", "405 AO-Sequenceable": "1", "PRIME-AcquisitionMethod": "Callbacks", "555 AO-Name": "AnalogIO", "PRIME-ShutterMode": "Pre-Exposure", "COM3-Handshaking": "Off", "445 AO-SupportsTriggering": "0", "ROI": "0-0-1200-1200", "COM9-Verbose": "1", "PRIME-Color": "OFF", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "PRIME-ShutterOpenDelay": "0", "COM1-Parity": "None", "Slice": 4, "SlicePosition": -10, "COM9-DelayBetweenCharsMs": "0.0000", "555-Name": "DA Shutter", "PIEZO-LudlSingleAxisName": "B", "XLIGHT Emission Wheel-Label": "725/40 (C)", "640 Shutter-Name": "AnalogIO", "640 AO-Sequenceable": "1", "520 Shutter-Demo": "No", "528 Shutter-Demo": "No", "470-Name": "DA Shutter", "520-DA Device": "520 Shutter", "XYStage-Description": "Ludl XY stage driver adapter", "445 Shutter-MinVolts": "0.0000", "528 AO-TriggerSampleRate": "100000", "405 AO-MinVolts": "0.0000", "405 Shutter-TriggerSequenceLength": "1000", "Height": 1200, "555 AO-Block voltage": "false", "640 Shutter-Volts": "5.0000", "PixelType": "GRAY16", "445 Shutter-Block voltage": "false", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "520 Shutter-TriggerSequenceLength": "1000", "COM9-Name": "COM9", "Core-SLM": "", "555 AO-Volts": "0.0000", "555 Shutter-Block voltage": "false", "XYStage-Speed": "1800.0000", "520 AO-MaxVolts": "5.0000", "528 Shutter-SupportsTriggering": "0", "COM1-Description": "Serial port driver (boost:asio)", "PRIME-MetadataEnabled": "No", "520 Shutter-MinVolts": "0.0000", "470 Shutter-Sequenceable": "1", "PRIME-PreampDelay": "0", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-MinVolts": "0.0000", "ChannelIndex": 3, "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Core-AutoShutter": "1", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "Exposure-ms": 520, "PRIME-PP 2 ENABLED": "Yes", "640 Shutter-TriggerSampleRate": "100000", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "470 Shutter-IOChannel": "Dev1/ao10", "405 AO-Demo": "No", "PixelSizeUm": 1.1, "470 AO-Demo": "No", "470 Shutter-MaxVolts": "5.0000", "Vincent-D3-Name": "Vincent-D3", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FileName": "img_000000000_Il-C_004.tif", "LudlController-Reset": "Operate", "PRIME-Exposure": "520.00", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "445-Name": "DA Shutter", "555 Shutter-Name": "AnalogIO", "520 Shutter-TriggerSampleRate": "100000", "445 Shutter-IOChannel": "Dev1/ao9", "PRIME-TransposeCorrection": "0", "XYStage-StepSize": "0.1000", "445-Description": "DA device that is used as a shutter", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 AO-IOChannel": "Dev1/ao7", "520 Shutter-Name": "AnalogIO", "COM3-DataBits": "8", "640 AO-Volts": "4.5000", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "555 Shutter-IOChannel": "Dev1/ao13", "WaitInterval": null, "520 Shutter-Sequenceable": "1", "YPositionUm": 20690.300000000003, "445-State": "0", "445-DA Device": "445 Shutter", "PRIME-CircularBufferAutoSize": "ON", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "555 AO-TriggerSampleRate": "100000", "445 Shutter-Volts": "0.0000", "XLIGHT Hub-Port": "COM3", "Core-AutoFocus": "", "COM4-Handshaking": "Off", "528 Shutter-MinVolts": "0.0000", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "445 Shutter-Name": "AnalogIO", "528 Shutter-Sequenceable": "1", "XYStage-TransposeMirrorX": "0", "XYStage-TransposeMirrorY": "0", "PRIME-ReadoutRate": "100MHz 16bit", "XYStage-Name": "Stage", "640 AO-SupportsTriggering": "0", "640-Name": "DA Shutter", "640 Shutter-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "640 AO-Block voltage": "false", "445 Shutter-Sequenceable": "1", "470 AO-MaxVolts": "5.0000", "COM4-BaudRate": "9600", "470-Description": "DA device that is used as a shutter", "520 Shutter-Description": "NI DAC", "PRIME-CCDTemperature": "-14.9600", "Time": "2019-12-17 21:59:01 -0500", "640 AO-TriggerSequenceLength": "1000", "470 AO-Name": "AnalogIO", "XLIGHT Disk slider-Label": "Disk Pos 40um", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "Binning": "1x1", "TE2000-ModelType": "1", "COM3-AnswerTimeout": "500.0000", "555 Shutter-SupportsTriggering": "0", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "TE2000-Port": "COM4", "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Description": "NI DAC", "COM4-AnswerTimeout": "500.0000", "COM1-DelayBetweenCharsMs": "0.0000", "FilterBlock-Label": "Confocal", "445 AO-Name": "AnalogIO", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-Timing-ReadoutTimeNs": "24672000.0000", "528 AO-Demo": "No", "520 AO-Sequenceable": "1", "555-Description": "DA device that is used as a shutter", "405-DA Device": "405 Shutter", "405 AO-TriggerSequenceLength": "1000", "640 AO-MinVolts": "0.0000", "640 Shutter-Block voltage": "false", "FrameIndex": 0, "PRIME-Port": "CMOS", "555 AO-MinVolts": "0.0000", "COM3-Description": "Serial port driver (boost:asio)", "528 AO-Description": "NI DAC", "555 AO-IOChannel": "Dev1/ao6", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "555 AO-TriggerInputLine": "/Dev1/PFI0", "PRIME-ActualInterval-ms": "374.3700", "LudlController-Port": "COM9", "445 Shutter-Demo": "No", "PRIME-Trigger-Expose Out-Mux": "1", "SliceIndex": 4, "PRIME-CCDTemperatureSetPoint": "-15.0000", "405 Shutter-MinVolts": "0.0000", "PRIME-ExposeOutMode": "First Row", "AxisPositions": null, "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Volts": "0.0000", "528-Name": "DA Shutter", "555-State": "0", "XLIGHT Dichroic-Label": "Di03-R635-t1", "445 AO-Sequenceable": "1", "405-Name": "DA Shutter", "405 AO-IOChannel": "Dev1/ao0", "Core-Camera": "PRIME", "Focus-Name": "Focus", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "405 Shutter-IOChannel": "Dev1/ao8", "640 AO-Demo": "No", "640 Shutter-SupportsTriggering": "0", "445 AO-Demo": "No", "555-DA Device": "555 Shutter", "470 Shutter-Description": "NI DAC", "528 AO-Volts": "0.0000", "COM1-DataBits": "8", "Vincent-D3-Address": "x", "520 AO-SupportsTriggering": "0", "405-Description": "DA device that is used as a shutter", "640 Shutter-MinVolts": "0.0000", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "405 AO-Name": "AnalogIO", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "640 AO-TriggerInputLine": "/Dev1/PFI0", "405 AO-TriggerInputLine": "/Dev1/PFI0", "640-Description": "DA device that is used as a shutter", "445 Shutter-TriggerSampleRate": "100000", "470 Shutter-TriggerSequenceLength": "1000", "COM1-StopBits": "1", "520 Shutter-SupportsTriggering": "0", "405-State": "0", "Core-Initialize": "1", "XLIGHT Emission Wheel-State": "7", "520 AO-Description": "NI DAC", "528 AO-TriggerInputLine": "/Dev1/PFI0", "445 Shutter-MaxVolts": "5.0000", "COM9-Handshaking": "Off", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "XLIGHT Spin motor-Label": "On (spinning)", "PRIME-PP 5 ENABLED": "No", "UUID": "f87fb106-0f50-46a3-953c-b7c59ae38c4b", "PRIME-TriggerMode": "Internal Trigger", "COM1-Handshaking": "Off", "PRIME-Timing-PostTriggerDelayNs": "49344000.0000", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "Focus-Position": "16479.8000", "528 Shutter-Block voltage": "false", "Channel": "Il-C", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "555 Shutter-Demo": "No", "405 Shutter-Demo": "No", "528 AO-Name": "AnalogIO", "520-State": "0", "Core-Galvo": "", "405 Shutter-TriggerSampleRate": "100000", "Focus-FocusStepSize_(nm)": "100", "PRIME-PixelType": "16bit", "COM4-DataBits": "8", "470 AO-IOChannel": "Dev1/ao2", "520 AO-MinVolts": "0.0000", "COM9-Parity": "None", "PRIME-CircularBufferEnabled": "ON", "PRIME-PP 6 Reset": "No", "640 AO-Description": "NI DAC", "Source": "PRIME", "445 AO-MinVolts": "0.0000", "PIEZO-Name": "Stage", "445 AO-Description": "NI DAC", "PRIME-CircularBufferFrameCount": "46", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "520 AO-Volts": "0.0000", "528 Shutter-TriggerSampleRate": "100000", "XPositionUm": 62321, "OpticalPath-State": "4", "PRIME-PP 3 THRESHOLD": "80", "PRIME-PVCAM Version": "3.7.1", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Gain": "1-HDR", "640 Shutter-TriggerSequenceLength": "1000", "528 AO-SupportsTriggering": "0", "445 AO-TriggerInputLine": "/Dev1/PFI0", "OpticalPath-Name": "OpticalPath", "PRIME-PP 4 ENABLED": "Yes", "PRIME-PVCAM Adapter Version": "1.3.29", "COM3-Name": "COM3", "405 AO-MaxVolts": "5.0000", "520 Shutter-Block voltage": "false", "PRIME-Timing-ClearingTimeNs": "49344000.0000", "Core-Focus": "PIEZO", "528 AO-Block voltage": "false", "TE2000-Description": "Nikon TE2000 hub", "528 AO-MinVolts": "0.0000", "COM4-Parity": "None", "Summary": { "Slices": 11, "ChContrastMin": [ 0, 0, 0, 0, 0, 0 ], "PixelAspect": 1, "SlicesFirst": true, "Interval_ms": 0, "Time": "2019-12-17 21:58:39 -0500", "Prefix": "seq_3", "InitialPositionList": [ { "Label": "Pos0", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.435], "Focus": [16480.2], "XYStage": [ 69822.40000000001, 20100.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos1", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.425], "Focus": [16480.3], "XYStage": [ 69004, 20101 ] }, "GridColumnIndex": 0 }, { "Label": "Pos2", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.44], "Focus": [16480.1], "XYStage": [ 63119.5, 20690.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos3", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.405], "Focus": [16480.2], "XYStage": [ 62321, 20690.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos4", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.415], "Focus": [16480], "XYStage": [ 56604.100000000006, 20688.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos5", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.42000000000002], "Focus": [16480.1], "XYStage": [ 56034.200000000004, 20688.800000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos6", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.455], "Focus": [16480], "XYStage": [ 50889.3, 20496.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos7", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.43], "Focus": [16480.2], "XYStage": [ 50101.600000000006, 20496.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos8", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.67000000000002], "Focus": [16479.9], "XYStage": [ 44818.600000000006, 20496.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos9", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.67000000000002], "Focus": [16480], "XYStage": [ 43897.9, 20436.800000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos10", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.675], "Focus": [16479.9], "XYStage": [ 39670.600000000006, 20664.9 ] }, "GridColumnIndex": 0 }, { "Label": "Pos11", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.69], "Focus": [16479.9], "XYStage": [ 38734, 20664.600000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos12", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.695], "Focus": [16480.1], "XYStage": [ 34037.200000000004, 20664.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos13", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.685], "Focus": [16480.1], "XYStage": [ 33152, 20664.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos14", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.69], "Focus": [16480.1], "XYStage": [ 28122.800000000003, 20664.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos15", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.675], "Focus": [16480], "XYStage": [ 27422.9, 20664.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos16", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.64000000000001], "Focus": [16480], "XYStage": [ 23005.2, 20664.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos17", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.69], "Focus": [16479.9], "XYStage": [ 21820.600000000002, 20409.600000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos18", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.41], "Focus": [16480.1], "XYStage": [ 73697.6, -13222.800000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos19", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.455], "Focus": [16480.1], "XYStage": [ 72765.8, -13223 ] }, "GridColumnIndex": 0 }, { "Label": "Pos20", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.4], "Focus": [16480.2], "XYStage": [ 67445.5, -12303.800000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos21", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.415], "Focus": [16480.2], "XYStage": [ 66613.3, -12303.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos22", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.415], "Focus": [16480.1], "XYStage": [ 61819.4, -11936.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos23", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.405], "Focus": [16480.2], "XYStage": [ 61325.700000000004, -11937.1 ] }, "GridColumnIndex": 0 }, { "Label": "Pos24", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.46], "Focus": [16480.2], "XYStage": [ 55377, -10974.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos25", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.445], "Focus": [16480.1], "XYStage": [ 54827, -11214.800000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos26", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.435], "Focus": [16480.2], "XYStage": [ 49937.100000000006, -10919.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos27", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.425], "Focus": [16480.1], "XYStage": [ 48764, -10919.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos28", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.42000000000002], "Focus": [16480.2], "XYStage": [ 43978.4, -10920.400000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos29", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.435], "Focus": [16480.2], "XYStage": [ 43106.3, -10920.400000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos30", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.405], "Focus": [16480.3], "XYStage": [ 38212.4, -10920.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos31", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.445], "Focus": [16480.2], "XYStage": [ 37254.9, -10920.400000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos32", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.44], "Focus": [16480.3], "XYStage": [ 32472.800000000003, -11294.400000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos33", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [166.95000000000002], "Focus": [16480.2], "XYStage": [ 31420.7, -11294.400000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos34", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [166.95000000000002], "Focus": [16480.2], "XYStage": [ 26881.5, -11640 ] }, "GridColumnIndex": 0 }, { "Label": "Pos35", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [166.98], "Focus": [16480.2], "XYStage": [ 25876.100000000002, -11641.1 ] }, "GridColumnIndex": 0 } ], "z-step_um": 10, "GridRow": 0, "BitDepth": 16, "Depth": 2, "Channels": 6, "Source": "Micro-Manager", "ChColors": [ -16711681, -256, -65281, -1, -1, -16776961 ], "MetadataVersion": 10, "KeepShutterOpenChannels": true, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536, 65536 ], "CustomIntervals_ms": [], "Height": 1200, "MicroManagerVersion": "1.4.23 20180219", "GridColumn": 0, "UUID": "8c8f9d20-b128-4577-9f91-89e70f22d319", "PixelType": "GRAY16", "Width": 1200, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\Yan\\20191217-YC113-S5S6", "Frames": 1, "Comment": "", "UserName": "Nikon", "PixelSize_um": 1.1, "ROI": [ 0, 0, 1200, 1200 ], "Date": "2019-12-17", "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC", "P-DAPI-high" ], "KeepShutterOpenSlices": true, "IJType": 1, "ComputerName": "ZadorConfocal2", "Positions": 36, "PositionIndex": 3, "CameraTimeout": 20000, "TimeFirst": false }, "640 AO-Name": "AnalogIO", "Vincent-D3-Command": "Close" }, "FrameKey-0-3-5": { "COM4-StopBits": "1", "528 AO-MaxVolts": "5.0000", "XLIGHT Excitation Wheel-State": "2", "470 Shutter-Name": "AnalogIO", "Core-Shutter": "640", "555 AO-Demo": "No", "XLIGHT Dichroic-State": "2", "XYStage-StepSize-Y": "0.1000", "ZPositionUm": 177.405, "XYStage-StepSize-X": "0.1000", "PRIME-FirmwareVersion": "20.28", "PRIME-ClearCycles": "2", "520-Name": "DA Shutter", "405 Shutter-Name": "AnalogIO", "PIEZO-StepSize": "0.0050", "PRIME-ClearMode": "Pre-Exposure", "470 AO-MinVolts": "0.0000", "405 AO-SupportsTriggering": "1", "CameraChannelIndex": 0, "XYStage-StartSpeed": "500.0000", "COM9-StopBits": "2", "405 AO-Description": "NI DAC", "COM4-DelayBetweenCharsMs": "0.0000", "445 AO-Block voltage": "false", "520 AO-TriggerInputLine": "/Dev1/PFI0", "555 AO-Description": "NI DAC", "520 AO-TriggerSampleRate": "100000", "COM4-Verbose": "1", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM4-Name": "COM4", "405 Shutter-Volts": "0.0000", "Vincent-D3-Port": "COM1", "PRIME-PP 4 MIN ADU AFFECTED": "200", "470 Shutter-MinVolts": "0.0000", "PRIME-PP 3 ENABLED": "Yes", "470 AO-Description": "NI DAC", "PRIME-Description": "PVCAM API device adapter", "520 Shutter-MaxVolts": "5.0000", "520 Shutter-Volts": "0.0000", "528 AO-Sequenceable": "1", "PRIME-Binning": "1x1", "555 Shutter-MaxVolts": "5.0000", "528 Shutter-TriggerSequenceLength": "1000", "405 Shutter-Block voltage": "false", "XLIGHT Spin motor-State": "1", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "Vincent-D3-Time to open (ms)": "35.0000", "Vincent-D3-Time to close (ms)": "35.0000", "445 AO-TriggerSampleRate": "100000", "640 Shutter-IOChannel": "Dev1/ao14", "555 AO-Sequenceable": "1", "520 AO-Name": "AnalogIO", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "470 AO-Block voltage": "false", "COM1-Verbose": "1", "COM9-AnswerTimeout": "10000.0000", "528 Shutter-Name": "AnalogIO", "PRIME-Trigger Timeout (secs)": "10", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "NextFrame": 0, "470 AO-TriggerSequenceLength": "1000", "PRIME-TransposeXY": "0", "445 AO-TriggerSequenceLength": "1000", "COM1-Name": "COM1", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "COM9-DataBits": "8", "COM1-AnswerTimeout": "500.0000", "Camera": "", "TE2000-Version": "V2.50-V2.50-V2.10", "528 Shutter-Volts": "0.0000", "XLIGHT Hub-Name": "XLIGHT Hub", "PIEZO-Description": "Ludl stage driver adapter", "PositionName": "Pos3", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSampleRate": "100000", "PRIME-FanSpeedSetpoint": "High", "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "405 AO-TriggerSampleRate": "100000", "470 Shutter-Block voltage": "false", "470-State": "0", "COM3-Parity": "None", "405 AO-Volts": "0.0000", "PRIME-TransposeMirrorY": "0", "PRIME-ChipName": "GS144BSI", "470 AO-Volts": "0.0000", "PRIME-TransposeMirrorX": "0", "445 Shutter-SupportsTriggering": "0", "470 Shutter-SupportsTriggering": "0", "XLIGHT Hub-Description": "XLIGHT hub", "PRIME-PreampOffLimit": "10000", "470 AO-Sequenceable": "1", "528 Shutter-MaxVolts": "5.0000", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "Core-XYStage": "XYStage", "470 AO-TriggerSampleRate": "100000", "PRIME-Timing-PreTriggerDelayNs": "24672000.0000", "528-DA Device": "528 Shutter", "Focus-Description": "Nikon TE2000 focus adapter", "FilterBlock-State": "5", "520 AO-Demo": "No", "COM3-DelayBetweenCharsMs": "0.0000", "470 Shutter-Volts": "0.0000", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "PRIME-PP 2 THRESHOLD": "125", "PRIME-PP 4 THRESHOLD": "75", "XYStage-Acceleration": "4.0000", "470 Shutter-Demo": "No", "PRIME-Name": "PMPCIECam00", "640-DA Device": "640 Shutter", "405 AO-Block voltage": "false", "ElapsedTime-ms": 155121, "PRIME-Offset": "100", "445 AO-IOChannel": "Dev1/ao1", "640 AO-MaxVolts": "5.0000", "PositionIndex": 3, "PRIME-PP 1 ENABLED": "Yes", "COM1-BaudRate": "9600", "640-State": "1", "COM3-Verbose": "1", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "LudlController-TransmissionDelay": "0", "PRIME-FullWellCapacity": "62000", "PRIME-SMARTStreamingEnabled": "No", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "640 Shutter-Description": "NI DAC", "528-Description": "DA device that is used as a shutter", "COM4-Description": "Serial port driver (boost:asio)", "405 Shutter-Description": "NI DAC", "TE2000-Name": "TE2000", "528 Shutter-Description": "NI DAC", "COM9-Description": "Serial port driver (boost:asio)", "LudlController-Name": "LudlController", "445 Shutter-TriggerSequenceLength": "1000", "PRIME-SerialNumber": "A18B203008", "445 AO-Volts": "0.0000", "PRIME-X-dimension": "1200", "PRIME-ShutterCloseDelay": "0", "XLIGHT Disk slider-State": "2", "Position": "Default", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 AO-IOChannel": "Dev1/ao5", "520 AO-IOChannel": "Dev1/ao4", "528 AO-TriggerSequenceLength": "1000", "405 Shutter-MaxVolts": "5.0000", "470-DA Device": "470 Shutter", "PRIME-FTCapable": "No", "Core-ChannelGroup": "Channels", "555 AO-SupportsTriggering": "0", "PRIME-Y-dimension": "1200", "Frame": 0, "555 Shutter-Sequenceable": "1", "PRIME-Timing-ExposureTimeNs": "9992000.0000", "Core-ImageProcessor": "", "528-State": "0", "520 AO-Block voltage": "false", "XLIGHT Excitation Wheel-Label": "QUAD", "640 Shutter-Demo": "No", "COM3-StopBits": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "520 Shutter-IOChannel": "Dev1/ao11", "Width": 1200, "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-SupportsTriggering": "0", "640 AO-TriggerSampleRate": "100000", "520-Description": "DA device that is used as a shutter", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "405 Shutter-Sequenceable": "1", "405 Shutter-SupportsTriggering": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-TriggerSampleRate": "100000", "640 Shutter-MaxVolts": "5.0000", "555 AO-MaxVolts": "5.0000", "520 AO-TriggerSequenceLength": "1000", "COM3-BaudRate": "9600", "COM9-BaudRate": "9600", "Vincent-D3-Channel #": "Ch. #1", "PRIME-PMode": "Normal", "OpticalPath-Label": "Left(100)", "BitDepth": 16, "555 AO-TriggerSequenceLength": "1000", "445 Shutter-Description": "NI DAC", "405 AO-Sequenceable": "1", "PRIME-AcquisitionMethod": "Callbacks", "555 AO-Name": "AnalogIO", "PRIME-ShutterMode": "Pre-Exposure", "COM3-Handshaking": "Off", "445 AO-SupportsTriggering": "0", "ROI": "0-0-1200-1200", "COM9-Verbose": "1", "PRIME-Color": "OFF", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "PRIME-ShutterOpenDelay": "0", "COM1-Parity": "None", "Slice": 5, "SlicePosition": 0, "COM9-DelayBetweenCharsMs": "0.0000", "555-Name": "DA Shutter", "PIEZO-LudlSingleAxisName": "B", "XLIGHT Emission Wheel-Label": "725/40 (C)", "640 Shutter-Name": "AnalogIO", "640 AO-Sequenceable": "1", "520 Shutter-Demo": "No", "528 Shutter-Demo": "No", "470-Name": "DA Shutter", "520-DA Device": "520 Shutter", "XYStage-Description": "Ludl XY stage driver adapter", "445 Shutter-MinVolts": "0.0000", "528 AO-TriggerSampleRate": "100000", "405 AO-MinVolts": "0.0000", "405 Shutter-TriggerSequenceLength": "1000", "Height": 1200, "555 AO-Block voltage": "false", "640 Shutter-Volts": "5.0000", "PixelType": "GRAY16", "445 Shutter-Block voltage": "false", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "520 Shutter-TriggerSequenceLength": "1000", "COM9-Name": "COM9", "Core-SLM": "", "555 AO-Volts": "0.0000", "555 Shutter-Block voltage": "false", "XYStage-Speed": "1800.0000", "520 AO-MaxVolts": "5.0000", "528 Shutter-SupportsTriggering": "0", "COM1-Description": "Serial port driver (boost:asio)", "PRIME-MetadataEnabled": "No", "520 Shutter-MinVolts": "0.0000", "470 Shutter-Sequenceable": "1", "PRIME-PreampDelay": "0", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-MinVolts": "0.0000", "ChannelIndex": 3, "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Core-AutoShutter": "1", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "Exposure-ms": 520, "PRIME-PP 2 ENABLED": "Yes", "640 Shutter-TriggerSampleRate": "100000", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "470 Shutter-IOChannel": "Dev1/ao10", "405 AO-Demo": "No", "PixelSizeUm": 1.1, "470 AO-Demo": "No", "470 Shutter-MaxVolts": "5.0000", "Vincent-D3-Name": "Vincent-D3", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FileName": "img_000000000_Il-C_005.tif", "LudlController-Reset": "Operate", "PRIME-Exposure": "520.00", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "445-Name": "DA Shutter", "555 Shutter-Name": "AnalogIO", "520 Shutter-TriggerSampleRate": "100000", "445 Shutter-IOChannel": "Dev1/ao9", "PRIME-TransposeCorrection": "0", "XYStage-StepSize": "0.1000", "445-Description": "DA device that is used as a shutter", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 AO-IOChannel": "Dev1/ao7", "520 Shutter-Name": "AnalogIO", "COM3-DataBits": "8", "640 AO-Volts": "4.5000", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "555 Shutter-IOChannel": "Dev1/ao13", "WaitInterval": null, "520 Shutter-Sequenceable": "1", "YPositionUm": 20690.300000000003, "445-State": "0", "445-DA Device": "445 Shutter", "PRIME-CircularBufferAutoSize": "ON", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "555 AO-TriggerSampleRate": "100000", "445 Shutter-Volts": "0.0000", "XLIGHT Hub-Port": "COM3", "Core-AutoFocus": "", "COM4-Handshaking": "Off", "528 Shutter-MinVolts": "0.0000", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "445 Shutter-Name": "AnalogIO", "528 Shutter-Sequenceable": "1", "XYStage-TransposeMirrorX": "0", "XYStage-TransposeMirrorY": "0", "PRIME-ReadoutRate": "100MHz 16bit", "XYStage-Name": "Stage", "640 AO-SupportsTriggering": "0", "640-Name": "DA Shutter", "640 Shutter-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "640 AO-Block voltage": "false", "445 Shutter-Sequenceable": "1", "470 AO-MaxVolts": "5.0000", "COM4-BaudRate": "9600", "470-Description": "DA device that is used as a shutter", "520 Shutter-Description": "NI DAC", "PRIME-CCDTemperature": "-14.9600", "Time": "2019-12-17 21:59:02 -0500", "640 AO-TriggerSequenceLength": "1000", "470 AO-Name": "AnalogIO", "XLIGHT Disk slider-Label": "Disk Pos 40um", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "Binning": "1x1", "TE2000-ModelType": "1", "COM3-AnswerTimeout": "500.0000", "555 Shutter-SupportsTriggering": "0", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "TE2000-Port": "COM4", "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Description": "NI DAC", "COM4-AnswerTimeout": "500.0000", "COM1-DelayBetweenCharsMs": "0.0000", "FilterBlock-Label": "Confocal", "445 AO-Name": "AnalogIO", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-Timing-ReadoutTimeNs": "24672000.0000", "528 AO-Demo": "No", "520 AO-Sequenceable": "1", "555-Description": "DA device that is used as a shutter", "405-DA Device": "405 Shutter", "405 AO-TriggerSequenceLength": "1000", "640 AO-MinVolts": "0.0000", "640 Shutter-Block voltage": "false", "FrameIndex": 0, "PRIME-Port": "CMOS", "555 AO-MinVolts": "0.0000", "COM3-Description": "Serial port driver (boost:asio)", "528 AO-Description": "NI DAC", "555 AO-IOChannel": "Dev1/ao6", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "555 AO-TriggerInputLine": "/Dev1/PFI0", "PRIME-ActualInterval-ms": "374.3700", "LudlController-Port": "COM9", "445 Shutter-Demo": "No", "PRIME-Trigger-Expose Out-Mux": "1", "SliceIndex": 5, "PRIME-CCDTemperatureSetPoint": "-15.0000", "405 Shutter-MinVolts": "0.0000", "PRIME-ExposeOutMode": "First Row", "AxisPositions": null, "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Volts": "0.0000", "528-Name": "DA Shutter", "555-State": "0", "XLIGHT Dichroic-Label": "Di03-R635-t1", "445 AO-Sequenceable": "1", "405-Name": "DA Shutter", "405 AO-IOChannel": "Dev1/ao0", "Core-Camera": "PRIME", "Focus-Name": "Focus", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "405 Shutter-IOChannel": "Dev1/ao8", "640 AO-Demo": "No", "640 Shutter-SupportsTriggering": "0", "445 AO-Demo": "No", "555-DA Device": "555 Shutter", "470 Shutter-Description": "NI DAC", "528 AO-Volts": "0.0000", "COM1-DataBits": "8", "Vincent-D3-Address": "x", "520 AO-SupportsTriggering": "0", "405-Description": "DA device that is used as a shutter", "640 Shutter-MinVolts": "0.0000", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "405 AO-Name": "AnalogIO", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "640 AO-TriggerInputLine": "/Dev1/PFI0", "405 AO-TriggerInputLine": "/Dev1/PFI0", "640-Description": "DA device that is used as a shutter", "445 Shutter-TriggerSampleRate": "100000", "470 Shutter-TriggerSequenceLength": "1000", "COM1-StopBits": "1", "520 Shutter-SupportsTriggering": "0", "405-State": "0", "Core-Initialize": "1", "XLIGHT Emission Wheel-State": "7", "520 AO-Description": "NI DAC", "528 AO-TriggerInputLine": "/Dev1/PFI0", "445 Shutter-MaxVolts": "5.0000", "COM9-Handshaking": "Off", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "XLIGHT Spin motor-Label": "On (spinning)", "PRIME-PP 5 ENABLED": "No", "UUID": "b8f2b056-cb88-4f60-9cdb-1689acbee9a9", "PRIME-TriggerMode": "Internal Trigger", "COM1-Handshaking": "Off", "PRIME-Timing-PostTriggerDelayNs": "49344000.0000", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "Focus-Position": "16479.8000", "528 Shutter-Block voltage": "false", "Channel": "Il-C", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "555 Shutter-Demo": "No", "405 Shutter-Demo": "No", "528 AO-Name": "AnalogIO", "520-State": "0", "Core-Galvo": "", "405 Shutter-TriggerSampleRate": "100000", "Focus-FocusStepSize_(nm)": "100", "PRIME-PixelType": "16bit", "COM4-DataBits": "8", "470 AO-IOChannel": "Dev1/ao2", "520 AO-MinVolts": "0.0000", "COM9-Parity": "None", "PRIME-CircularBufferEnabled": "ON", "PRIME-PP 6 Reset": "No", "640 AO-Description": "NI DAC", "Source": "PRIME", "445 AO-MinVolts": "0.0000", "PIEZO-Name": "Stage", "445 AO-Description": "NI DAC", "PRIME-CircularBufferFrameCount": "46", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "520 AO-Volts": "0.0000", "528 Shutter-TriggerSampleRate": "100000", "XPositionUm": 62321, "OpticalPath-State": "4", "PRIME-PP 3 THRESHOLD": "80", "PRIME-PVCAM Version": "3.7.1", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Gain": "1-HDR", "640 Shutter-TriggerSequenceLength": "1000", "528 AO-SupportsTriggering": "0", "445 AO-TriggerInputLine": "/Dev1/PFI0", "OpticalPath-Name": "OpticalPath", "PRIME-PP 4 ENABLED": "Yes", "PRIME-PVCAM Adapter Version": "1.3.29", "COM3-Name": "COM3", "405 AO-MaxVolts": "5.0000", "520 Shutter-Block voltage": "false", "PRIME-Timing-ClearingTimeNs": "49344000.0000", "Core-Focus": "PIEZO", "528 AO-Block voltage": "false", "TE2000-Description": "Nikon TE2000 hub", "528 AO-MinVolts": "0.0000", "COM4-Parity": "None", "Summary": { "Slices": 11, "ChContrastMin": [ 0, 0, 0, 0, 0, 0 ], "PixelAspect": 1, "SlicesFirst": true, "Interval_ms": 0, "Time": "2019-12-17 21:58:39 -0500", "Prefix": "seq_3", "InitialPositionList": [ { "Label": "Pos0", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.435], "Focus": [16480.2], "XYStage": [ 69822.40000000001, 20100.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos1", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.425], "Focus": [16480.3], "XYStage": [ 69004, 20101 ] }, "GridColumnIndex": 0 }, { "Label": "Pos2", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.44], "Focus": [16480.1], "XYStage": [ 63119.5, 20690.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos3", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.405], "Focus": [16480.2], "XYStage": [ 62321, 20690.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos4", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.415], "Focus": [16480], "XYStage": [ 56604.100000000006, 20688.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos5", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.42000000000002], "Focus": [16480.1], "XYStage": [ 56034.200000000004, 20688.800000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos6", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.455], "Focus": [16480], "XYStage": [ 50889.3, 20496.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos7", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.43], "Focus": [16480.2], "XYStage": [ 50101.600000000006, 20496.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos8", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.67000000000002], "Focus": [16479.9], "XYStage": [ 44818.600000000006, 20496.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos9", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.67000000000002], "Focus": [16480], "XYStage": [ 43897.9, 20436.800000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos10", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.675], "Focus": [16479.9], "XYStage": [ 39670.600000000006, 20664.9 ] }, "GridColumnIndex": 0 }, { "Label": "Pos11", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.69], "Focus": [16479.9], "XYStage": [ 38734, 20664.600000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos12", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.695], "Focus": [16480.1], "XYStage": [ 34037.200000000004, 20664.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos13", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.685], "Focus": [16480.1], "XYStage": [ 33152, 20664.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos14", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.69], "Focus": [16480.1], "XYStage": [ 28122.800000000003, 20664.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos15", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.675], "Focus": [16480], "XYStage": [ 27422.9, 20664.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos16", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.64000000000001], "Focus": [16480], "XYStage": [ 23005.2, 20664.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos17", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.69], "Focus": [16479.9], "XYStage": [ 21820.600000000002, 20409.600000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos18", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.41], "Focus": [16480.1], "XYStage": [ 73697.6, -13222.800000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos19", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.455], "Focus": [16480.1], "XYStage": [ 72765.8, -13223 ] }, "GridColumnIndex": 0 }, { "Label": "Pos20", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.4], "Focus": [16480.2], "XYStage": [ 67445.5, -12303.800000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos21", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.415], "Focus": [16480.2], "XYStage": [ 66613.3, -12303.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos22", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.415], "Focus": [16480.1], "XYStage": [ 61819.4, -11936.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos23", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.405], "Focus": [16480.2], "XYStage": [ 61325.700000000004, -11937.1 ] }, "GridColumnIndex": 0 }, { "Label": "Pos24", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.46], "Focus": [16480.2], "XYStage": [ 55377, -10974.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos25", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.445], "Focus": [16480.1], "XYStage": [ 54827, -11214.800000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos26", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.435], "Focus": [16480.2], "XYStage": [ 49937.100000000006, -10919.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos27", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.425], "Focus": [16480.1], "XYStage": [ 48764, -10919.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos28", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.42000000000002], "Focus": [16480.2], "XYStage": [ 43978.4, -10920.400000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos29", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.435], "Focus": [16480.2], "XYStage": [ 43106.3, -10920.400000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos30", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.405], "Focus": [16480.3], "XYStage": [ 38212.4, -10920.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos31", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.445], "Focus": [16480.2], "XYStage": [ 37254.9, -10920.400000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos32", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.44], "Focus": [16480.3], "XYStage": [ 32472.800000000003, -11294.400000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos33", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [166.95000000000002], "Focus": [16480.2], "XYStage": [ 31420.7, -11294.400000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos34", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [166.95000000000002], "Focus": [16480.2], "XYStage": [ 26881.5, -11640 ] }, "GridColumnIndex": 0 }, { "Label": "Pos35", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [166.98], "Focus": [16480.2], "XYStage": [ 25876.100000000002, -11641.1 ] }, "GridColumnIndex": 0 } ], "z-step_um": 10, "GridRow": 0, "BitDepth": 16, "Depth": 2, "Channels": 6, "Source": "Micro-Manager", "ChColors": [ -16711681, -256, -65281, -1, -1, -16776961 ], "MetadataVersion": 10, "KeepShutterOpenChannels": true, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536, 65536 ], "CustomIntervals_ms": [], "Height": 1200, "MicroManagerVersion": "1.4.23 20180219", "GridColumn": 0, "UUID": "8c8f9d20-b128-4577-9f91-89e70f22d319", "PixelType": "GRAY16", "Width": 1200, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\Yan\\20191217-YC113-S5S6", "Frames": 1, "Comment": "", "UserName": "Nikon", "PixelSize_um": 1.1, "ROI": [ 0, 0, 1200, 1200 ], "Date": "2019-12-17", "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC", "P-DAPI-high" ], "KeepShutterOpenSlices": true, "IJType": 1, "ComputerName": "ZadorConfocal2", "Positions": 36, "PositionIndex": 3, "CameraTimeout": 20000, "TimeFirst": false }, "640 AO-Name": "AnalogIO", "Vincent-D3-Command": "Close" }, "FrameKey-0-3-6": { "COM4-StopBits": "1", "528 AO-MaxVolts": "5.0000", "XLIGHT Excitation Wheel-State": "2", "470 Shutter-Name": "AnalogIO", "Core-Shutter": "640", "555 AO-Demo": "No", "XLIGHT Dichroic-State": "2", "XYStage-StepSize-Y": "0.1000", "ZPositionUm": 187.405, "XYStage-StepSize-X": "0.1000", "PRIME-FirmwareVersion": "20.28", "PRIME-ClearCycles": "2", "520-Name": "DA Shutter", "405 Shutter-Name": "AnalogIO", "PIEZO-StepSize": "0.0050", "PRIME-ClearMode": "Pre-Exposure", "470 AO-MinVolts": "0.0000", "405 AO-SupportsTriggering": "1", "CameraChannelIndex": 0, "XYStage-StartSpeed": "500.0000", "COM9-StopBits": "2", "405 AO-Description": "NI DAC", "COM4-DelayBetweenCharsMs": "0.0000", "445 AO-Block voltage": "false", "520 AO-TriggerInputLine": "/Dev1/PFI0", "555 AO-Description": "NI DAC", "520 AO-TriggerSampleRate": "100000", "COM4-Verbose": "1", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM4-Name": "COM4", "405 Shutter-Volts": "0.0000", "Vincent-D3-Port": "COM1", "PRIME-PP 4 MIN ADU AFFECTED": "200", "470 Shutter-MinVolts": "0.0000", "PRIME-PP 3 ENABLED": "Yes", "470 AO-Description": "NI DAC", "PRIME-Description": "PVCAM API device adapter", "520 Shutter-MaxVolts": "5.0000", "520 Shutter-Volts": "0.0000", "528 AO-Sequenceable": "1", "PRIME-Binning": "1x1", "555 Shutter-MaxVolts": "5.0000", "528 Shutter-TriggerSequenceLength": "1000", "405 Shutter-Block voltage": "false", "XLIGHT Spin motor-State": "1", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "Vincent-D3-Time to open (ms)": "35.0000", "Vincent-D3-Time to close (ms)": "35.0000", "445 AO-TriggerSampleRate": "100000", "640 Shutter-IOChannel": "Dev1/ao14", "555 AO-Sequenceable": "1", "520 AO-Name": "AnalogIO", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "470 AO-Block voltage": "false", "COM1-Verbose": "1", "COM9-AnswerTimeout": "10000.0000", "528 Shutter-Name": "AnalogIO", "PRIME-Trigger Timeout (secs)": "10", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "NextFrame": 0, "470 AO-TriggerSequenceLength": "1000", "PRIME-TransposeXY": "0", "445 AO-TriggerSequenceLength": "1000", "COM1-Name": "COM1", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "COM9-DataBits": "8", "COM1-AnswerTimeout": "500.0000", "Camera": "", "TE2000-Version": "V2.50-V2.50-V2.10", "528 Shutter-Volts": "0.0000", "XLIGHT Hub-Name": "XLIGHT Hub", "PIEZO-Description": "Ludl stage driver adapter", "PositionName": "Pos3", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSampleRate": "100000", "PRIME-FanSpeedSetpoint": "High", "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "405 AO-TriggerSampleRate": "100000", "470 Shutter-Block voltage": "false", "470-State": "0", "COM3-Parity": "None", "405 AO-Volts": "0.0000", "PRIME-TransposeMirrorY": "0", "PRIME-ChipName": "GS144BSI", "470 AO-Volts": "0.0000", "PRIME-TransposeMirrorX": "0", "445 Shutter-SupportsTriggering": "0", "470 Shutter-SupportsTriggering": "0", "XLIGHT Hub-Description": "XLIGHT hub", "PRIME-PreampOffLimit": "10000", "470 AO-Sequenceable": "1", "528 Shutter-MaxVolts": "5.0000", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "Core-XYStage": "XYStage", "470 AO-TriggerSampleRate": "100000", "PRIME-Timing-PreTriggerDelayNs": "24672000.0000", "528-DA Device": "528 Shutter", "Focus-Description": "Nikon TE2000 focus adapter", "FilterBlock-State": "5", "520 AO-Demo": "No", "COM3-DelayBetweenCharsMs": "0.0000", "470 Shutter-Volts": "0.0000", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "PRIME-PP 2 THRESHOLD": "125", "PRIME-PP 4 THRESHOLD": "75", "XYStage-Acceleration": "4.0000", "470 Shutter-Demo": "No", "PRIME-Name": "PMPCIECam00", "640-DA Device": "640 Shutter", "405 AO-Block voltage": "false", "ElapsedTime-ms": 155873, "PRIME-Offset": "100", "445 AO-IOChannel": "Dev1/ao1", "640 AO-MaxVolts": "5.0000", "PositionIndex": 3, "PRIME-PP 1 ENABLED": "Yes", "COM1-BaudRate": "9600", "640-State": "1", "COM3-Verbose": "1", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "LudlController-TransmissionDelay": "0", "PRIME-FullWellCapacity": "62000", "PRIME-SMARTStreamingEnabled": "No", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "640 Shutter-Description": "NI DAC", "528-Description": "DA device that is used as a shutter", "COM4-Description": "Serial port driver (boost:asio)", "405 Shutter-Description": "NI DAC", "TE2000-Name": "TE2000", "528 Shutter-Description": "NI DAC", "COM9-Description": "Serial port driver (boost:asio)", "LudlController-Name": "LudlController", "445 Shutter-TriggerSequenceLength": "1000", "PRIME-SerialNumber": "A18B203008", "445 AO-Volts": "0.0000", "PRIME-X-dimension": "1200", "PRIME-ShutterCloseDelay": "0", "XLIGHT Disk slider-State": "2", "Position": "Default", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 AO-IOChannel": "Dev1/ao5", "520 AO-IOChannel": "Dev1/ao4", "528 AO-TriggerSequenceLength": "1000", "405 Shutter-MaxVolts": "5.0000", "470-DA Device": "470 Shutter", "PRIME-FTCapable": "No", "Core-ChannelGroup": "Channels", "555 AO-SupportsTriggering": "0", "PRIME-Y-dimension": "1200", "Frame": 0, "555 Shutter-Sequenceable": "1", "PRIME-Timing-ExposureTimeNs": "9992000.0000", "Core-ImageProcessor": "", "528-State": "0", "520 AO-Block voltage": "false", "XLIGHT Excitation Wheel-Label": "QUAD", "640 Shutter-Demo": "No", "COM3-StopBits": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "520 Shutter-IOChannel": "Dev1/ao11", "Width": 1200, "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-SupportsTriggering": "0", "640 AO-TriggerSampleRate": "100000", "520-Description": "DA device that is used as a shutter", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "405 Shutter-Sequenceable": "1", "405 Shutter-SupportsTriggering": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-TriggerSampleRate": "100000", "640 Shutter-MaxVolts": "5.0000", "555 AO-MaxVolts": "5.0000", "520 AO-TriggerSequenceLength": "1000", "COM3-BaudRate": "9600", "COM9-BaudRate": "9600", "Vincent-D3-Channel #": "Ch. #1", "PRIME-PMode": "Normal", "OpticalPath-Label": "Left(100)", "BitDepth": 16, "555 AO-TriggerSequenceLength": "1000", "445 Shutter-Description": "NI DAC", "405 AO-Sequenceable": "1", "PRIME-AcquisitionMethod": "Callbacks", "555 AO-Name": "AnalogIO", "PRIME-ShutterMode": "Pre-Exposure", "COM3-Handshaking": "Off", "445 AO-SupportsTriggering": "0", "ROI": "0-0-1200-1200", "COM9-Verbose": "1", "PRIME-Color": "OFF", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "PRIME-ShutterOpenDelay": "0", "COM1-Parity": "None", "Slice": 6, "SlicePosition": 10, "COM9-DelayBetweenCharsMs": "0.0000", "555-Name": "DA Shutter", "PIEZO-LudlSingleAxisName": "B", "XLIGHT Emission Wheel-Label": "725/40 (C)", "640 Shutter-Name": "AnalogIO", "640 AO-Sequenceable": "1", "520 Shutter-Demo": "No", "528 Shutter-Demo": "No", "470-Name": "DA Shutter", "520-DA Device": "520 Shutter", "XYStage-Description": "Ludl XY stage driver adapter", "445 Shutter-MinVolts": "0.0000", "528 AO-TriggerSampleRate": "100000", "405 AO-MinVolts": "0.0000", "405 Shutter-TriggerSequenceLength": "1000", "Height": 1200, "555 AO-Block voltage": "false", "640 Shutter-Volts": "5.0000", "PixelType": "GRAY16", "445 Shutter-Block voltage": "false", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "520 Shutter-TriggerSequenceLength": "1000", "COM9-Name": "COM9", "Core-SLM": "", "555 AO-Volts": "0.0000", "555 Shutter-Block voltage": "false", "XYStage-Speed": "1800.0000", "520 AO-MaxVolts": "5.0000", "528 Shutter-SupportsTriggering": "0", "COM1-Description": "Serial port driver (boost:asio)", "PRIME-MetadataEnabled": "No", "520 Shutter-MinVolts": "0.0000", "470 Shutter-Sequenceable": "1", "PRIME-PreampDelay": "0", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-MinVolts": "0.0000", "ChannelIndex": 3, "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Core-AutoShutter": "1", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "Exposure-ms": 520, "PRIME-PP 2 ENABLED": "Yes", "640 Shutter-TriggerSampleRate": "100000", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "470 Shutter-IOChannel": "Dev1/ao10", "405 AO-Demo": "No", "PixelSizeUm": 1.1, "470 AO-Demo": "No", "470 Shutter-MaxVolts": "5.0000", "Vincent-D3-Name": "Vincent-D3", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FileName": "img_000000000_Il-C_006.tif", "LudlController-Reset": "Operate", "PRIME-Exposure": "520.00", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "445-Name": "DA Shutter", "555 Shutter-Name": "AnalogIO", "520 Shutter-TriggerSampleRate": "100000", "445 Shutter-IOChannel": "Dev1/ao9", "PRIME-TransposeCorrection": "0", "XYStage-StepSize": "0.1000", "445-Description": "DA device that is used as a shutter", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 AO-IOChannel": "Dev1/ao7", "520 Shutter-Name": "AnalogIO", "COM3-DataBits": "8", "640 AO-Volts": "4.5000", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "555 Shutter-IOChannel": "Dev1/ao13", "WaitInterval": null, "520 Shutter-Sequenceable": "1", "YPositionUm": 20690.300000000003, "445-State": "0", "445-DA Device": "445 Shutter", "PRIME-CircularBufferAutoSize": "ON", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "555 AO-TriggerSampleRate": "100000", "445 Shutter-Volts": "0.0000", "XLIGHT Hub-Port": "COM3", "Core-AutoFocus": "", "COM4-Handshaking": "Off", "528 Shutter-MinVolts": "0.0000", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "445 Shutter-Name": "AnalogIO", "528 Shutter-Sequenceable": "1", "XYStage-TransposeMirrorX": "0", "XYStage-TransposeMirrorY": "0", "PRIME-ReadoutRate": "100MHz 16bit", "XYStage-Name": "Stage", "640 AO-SupportsTriggering": "0", "640-Name": "DA Shutter", "640 Shutter-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "640 AO-Block voltage": "false", "445 Shutter-Sequenceable": "1", "470 AO-MaxVolts": "5.0000", "COM4-BaudRate": "9600", "470-Description": "DA device that is used as a shutter", "520 Shutter-Description": "NI DAC", "PRIME-CCDTemperature": "-14.9600", "Time": "2019-12-17 21:59:03 -0500", "640 AO-TriggerSequenceLength": "1000", "470 AO-Name": "AnalogIO", "XLIGHT Disk slider-Label": "Disk Pos 40um", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "Binning": "1x1", "TE2000-ModelType": "1", "COM3-AnswerTimeout": "500.0000", "555 Shutter-SupportsTriggering": "0", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "TE2000-Port": "COM4", "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Description": "NI DAC", "COM4-AnswerTimeout": "500.0000", "COM1-DelayBetweenCharsMs": "0.0000", "FilterBlock-Label": "Confocal", "445 AO-Name": "AnalogIO", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-Timing-ReadoutTimeNs": "24672000.0000", "528 AO-Demo": "No", "520 AO-Sequenceable": "1", "555-Description": "DA device that is used as a shutter", "405-DA Device": "405 Shutter", "405 AO-TriggerSequenceLength": "1000", "640 AO-MinVolts": "0.0000", "640 Shutter-Block voltage": "false", "FrameIndex": 0, "PRIME-Port": "CMOS", "555 AO-MinVolts": "0.0000", "COM3-Description": "Serial port driver (boost:asio)", "528 AO-Description": "NI DAC", "555 AO-IOChannel": "Dev1/ao6", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "555 AO-TriggerInputLine": "/Dev1/PFI0", "PRIME-ActualInterval-ms": "374.3700", "LudlController-Port": "COM9", "445 Shutter-Demo": "No", "PRIME-Trigger-Expose Out-Mux": "1", "SliceIndex": 6, "PRIME-CCDTemperatureSetPoint": "-15.0000", "405 Shutter-MinVolts": "0.0000", "PRIME-ExposeOutMode": "First Row", "AxisPositions": null, "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Volts": "0.0000", "528-Name": "DA Shutter", "555-State": "0", "XLIGHT Dichroic-Label": "Di03-R635-t1", "445 AO-Sequenceable": "1", "405-Name": "DA Shutter", "405 AO-IOChannel": "Dev1/ao0", "Core-Camera": "PRIME", "Focus-Name": "Focus", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "405 Shutter-IOChannel": "Dev1/ao8", "640 AO-Demo": "No", "640 Shutter-SupportsTriggering": "0", "445 AO-Demo": "No", "555-DA Device": "555 Shutter", "470 Shutter-Description": "NI DAC", "528 AO-Volts": "0.0000", "COM1-DataBits": "8", "Vincent-D3-Address": "x", "520 AO-SupportsTriggering": "0", "405-Description": "DA device that is used as a shutter", "640 Shutter-MinVolts": "0.0000", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "405 AO-Name": "AnalogIO", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "640 AO-TriggerInputLine": "/Dev1/PFI0", "405 AO-TriggerInputLine": "/Dev1/PFI0", "640-Description": "DA device that is used as a shutter", "445 Shutter-TriggerSampleRate": "100000", "470 Shutter-TriggerSequenceLength": "1000", "COM1-StopBits": "1", "520 Shutter-SupportsTriggering": "0", "405-State": "0", "Core-Initialize": "1", "XLIGHT Emission Wheel-State": "7", "520 AO-Description": "NI DAC", "528 AO-TriggerInputLine": "/Dev1/PFI0", "445 Shutter-MaxVolts": "5.0000", "COM9-Handshaking": "Off", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "XLIGHT Spin motor-Label": "On (spinning)", "PRIME-PP 5 ENABLED": "No", "UUID": "bdb94654-69aa-4f32-b1f0-d2a445873b2e", "PRIME-TriggerMode": "Internal Trigger", "COM1-Handshaking": "Off", "PRIME-Timing-PostTriggerDelayNs": "49344000.0000", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "Focus-Position": "16479.8000", "528 Shutter-Block voltage": "false", "Channel": "Il-C", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "555 Shutter-Demo": "No", "405 Shutter-Demo": "No", "528 AO-Name": "AnalogIO", "520-State": "0", "Core-Galvo": "", "405 Shutter-TriggerSampleRate": "100000", "Focus-FocusStepSize_(nm)": "100", "PRIME-PixelType": "16bit", "COM4-DataBits": "8", "470 AO-IOChannel": "Dev1/ao2", "520 AO-MinVolts": "0.0000", "COM9-Parity": "None", "PRIME-CircularBufferEnabled": "ON", "PRIME-PP 6 Reset": "No", "640 AO-Description": "NI DAC", "Source": "PRIME", "445 AO-MinVolts": "0.0000", "PIEZO-Name": "Stage", "445 AO-Description": "NI DAC", "PRIME-CircularBufferFrameCount": "46", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "520 AO-Volts": "0.0000", "528 Shutter-TriggerSampleRate": "100000", "XPositionUm": 62321, "OpticalPath-State": "4", "PRIME-PP 3 THRESHOLD": "80", "PRIME-PVCAM Version": "3.7.1", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Gain": "1-HDR", "640 Shutter-TriggerSequenceLength": "1000", "528 AO-SupportsTriggering": "0", "445 AO-TriggerInputLine": "/Dev1/PFI0", "OpticalPath-Name": "OpticalPath", "PRIME-PP 4 ENABLED": "Yes", "PRIME-PVCAM Adapter Version": "1.3.29", "COM3-Name": "COM3", "405 AO-MaxVolts": "5.0000", "520 Shutter-Block voltage": "false", "PRIME-Timing-ClearingTimeNs": "49344000.0000", "Core-Focus": "PIEZO", "528 AO-Block voltage": "false", "TE2000-Description": "Nikon TE2000 hub", "528 AO-MinVolts": "0.0000", "COM4-Parity": "None", "Summary": { "Slices": 11, "ChContrastMin": [ 0, 0, 0, 0, 0, 0 ], "PixelAspect": 1, "SlicesFirst": true, "Interval_ms": 0, "Time": "2019-12-17 21:58:39 -0500", "Prefix": "seq_3", "InitialPositionList": [ { "Label": "Pos0", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.435], "Focus": [16480.2], "XYStage": [ 69822.40000000001, 20100.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos1", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.425], "Focus": [16480.3], "XYStage": [ 69004, 20101 ] }, "GridColumnIndex": 0 }, { "Label": "Pos2", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.44], "Focus": [16480.1], "XYStage": [ 63119.5, 20690.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos3", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.405], "Focus": [16480.2], "XYStage": [ 62321, 20690.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos4", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.415], "Focus": [16480], "XYStage": [ 56604.100000000006, 20688.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos5", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.42000000000002], "Focus": [16480.1], "XYStage": [ 56034.200000000004, 20688.800000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos6", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.455], "Focus": [16480], "XYStage": [ 50889.3, 20496.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos7", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.43], "Focus": [16480.2], "XYStage": [ 50101.600000000006, 20496.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos8", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.67000000000002], "Focus": [16479.9], "XYStage": [ 44818.600000000006, 20496.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos9", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.67000000000002], "Focus": [16480], "XYStage": [ 43897.9, 20436.800000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos10", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.675], "Focus": [16479.9], "XYStage": [ 39670.600000000006, 20664.9 ] }, "GridColumnIndex": 0 }, { "Label": "Pos11", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.69], "Focus": [16479.9], "XYStage": [ 38734, 20664.600000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos12", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.695], "Focus": [16480.1], "XYStage": [ 34037.200000000004, 20664.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos13", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.685], "Focus": [16480.1], "XYStage": [ 33152, 20664.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos14", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.69], "Focus": [16480.1], "XYStage": [ 28122.800000000003, 20664.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos15", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.675], "Focus": [16480], "XYStage": [ 27422.9, 20664.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos16", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.64000000000001], "Focus": [16480], "XYStage": [ 23005.2, 20664.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos17", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.69], "Focus": [16479.9], "XYStage": [ 21820.600000000002, 20409.600000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos18", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.41], "Focus": [16480.1], "XYStage": [ 73697.6, -13222.800000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos19", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.455], "Focus": [16480.1], "XYStage": [ 72765.8, -13223 ] }, "GridColumnIndex": 0 }, { "Label": "Pos20", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.4], "Focus": [16480.2], "XYStage": [ 67445.5, -12303.800000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos21", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.415], "Focus": [16480.2], "XYStage": [ 66613.3, -12303.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos22", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.415], "Focus": [16480.1], "XYStage": [ 61819.4, -11936.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos23", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.405], "Focus": [16480.2], "XYStage": [ 61325.700000000004, -11937.1 ] }, "GridColumnIndex": 0 }, { "Label": "Pos24", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.46], "Focus": [16480.2], "XYStage": [ 55377, -10974.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos25", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.445], "Focus": [16480.1], "XYStage": [ 54827, -11214.800000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos26", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.435], "Focus": [16480.2], "XYStage": [ 49937.100000000006, -10919.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos27", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.425], "Focus": [16480.1], "XYStage": [ 48764, -10919.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos28", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.42000000000002], "Focus": [16480.2], "XYStage": [ 43978.4, -10920.400000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos29", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.435], "Focus": [16480.2], "XYStage": [ 43106.3, -10920.400000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos30", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.405], "Focus": [16480.3], "XYStage": [ 38212.4, -10920.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos31", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.445], "Focus": [16480.2], "XYStage": [ 37254.9, -10920.400000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos32", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.44], "Focus": [16480.3], "XYStage": [ 32472.800000000003, -11294.400000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos33", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [166.95000000000002], "Focus": [16480.2], "XYStage": [ 31420.7, -11294.400000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos34", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [166.95000000000002], "Focus": [16480.2], "XYStage": [ 26881.5, -11640 ] }, "GridColumnIndex": 0 }, { "Label": "Pos35", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [166.98], "Focus": [16480.2], "XYStage": [ 25876.100000000002, -11641.1 ] }, "GridColumnIndex": 0 } ], "z-step_um": 10, "GridRow": 0, "BitDepth": 16, "Depth": 2, "Channels": 6, "Source": "Micro-Manager", "ChColors": [ -16711681, -256, -65281, -1, -1, -16776961 ], "MetadataVersion": 10, "KeepShutterOpenChannels": true, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536, 65536 ], "CustomIntervals_ms": [], "Height": 1200, "MicroManagerVersion": "1.4.23 20180219", "GridColumn": 0, "UUID": "8c8f9d20-b128-4577-9f91-89e70f22d319", "PixelType": "GRAY16", "Width": 1200, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\Yan\\20191217-YC113-S5S6", "Frames": 1, "Comment": "", "UserName": "Nikon", "PixelSize_um": 1.1, "ROI": [ 0, 0, 1200, 1200 ], "Date": "2019-12-17", "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC", "P-DAPI-high" ], "KeepShutterOpenSlices": true, "IJType": 1, "ComputerName": "ZadorConfocal2", "Positions": 36, "PositionIndex": 3, "CameraTimeout": 20000, "TimeFirst": false }, "640 AO-Name": "AnalogIO", "Vincent-D3-Command": "Close" }, "FrameKey-0-3-7": { "COM4-StopBits": "1", "528 AO-MaxVolts": "5.0000", "XLIGHT Excitation Wheel-State": "2", "470 Shutter-Name": "AnalogIO", "Core-Shutter": "640", "555 AO-Demo": "No", "XLIGHT Dichroic-State": "2", "XYStage-StepSize-Y": "0.1000", "ZPositionUm": 197.405, "XYStage-StepSize-X": "0.1000", "PRIME-FirmwareVersion": "20.28", "PRIME-ClearCycles": "2", "520-Name": "DA Shutter", "405 Shutter-Name": "AnalogIO", "PIEZO-StepSize": "0.0050", "PRIME-ClearMode": "Pre-Exposure", "470 AO-MinVolts": "0.0000", "405 AO-SupportsTriggering": "1", "CameraChannelIndex": 0, "XYStage-StartSpeed": "500.0000", "COM9-StopBits": "2", "405 AO-Description": "NI DAC", "COM4-DelayBetweenCharsMs": "0.0000", "445 AO-Block voltage": "false", "520 AO-TriggerInputLine": "/Dev1/PFI0", "555 AO-Description": "NI DAC", "520 AO-TriggerSampleRate": "100000", "COM4-Verbose": "1", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM4-Name": "COM4", "405 Shutter-Volts": "0.0000", "Vincent-D3-Port": "COM1", "PRIME-PP 4 MIN ADU AFFECTED": "200", "470 Shutter-MinVolts": "0.0000", "PRIME-PP 3 ENABLED": "Yes", "470 AO-Description": "NI DAC", "PRIME-Description": "PVCAM API device adapter", "520 Shutter-MaxVolts": "5.0000", "520 Shutter-Volts": "0.0000", "528 AO-Sequenceable": "1", "PRIME-Binning": "1x1", "555 Shutter-MaxVolts": "5.0000", "528 Shutter-TriggerSequenceLength": "1000", "405 Shutter-Block voltage": "false", "XLIGHT Spin motor-State": "1", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "Vincent-D3-Time to open (ms)": "35.0000", "Vincent-D3-Time to close (ms)": "35.0000", "445 AO-TriggerSampleRate": "100000", "640 Shutter-IOChannel": "Dev1/ao14", "555 AO-Sequenceable": "1", "520 AO-Name": "AnalogIO", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "470 AO-Block voltage": "false", "COM1-Verbose": "1", "COM9-AnswerTimeout": "10000.0000", "528 Shutter-Name": "AnalogIO", "PRIME-Trigger Timeout (secs)": "10", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "NextFrame": 0, "470 AO-TriggerSequenceLength": "1000", "PRIME-TransposeXY": "0", "445 AO-TriggerSequenceLength": "1000", "COM1-Name": "COM1", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "COM9-DataBits": "8", "COM1-AnswerTimeout": "500.0000", "Camera": "", "TE2000-Version": "V2.50-V2.50-V2.10", "528 Shutter-Volts": "0.0000", "XLIGHT Hub-Name": "XLIGHT Hub", "PIEZO-Description": "Ludl stage driver adapter", "PositionName": "Pos3", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSampleRate": "100000", "PRIME-FanSpeedSetpoint": "High", "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "405 AO-TriggerSampleRate": "100000", "470 Shutter-Block voltage": "false", "470-State": "0", "COM3-Parity": "None", "405 AO-Volts": "0.0000", "PRIME-TransposeMirrorY": "0", "PRIME-ChipName": "GS144BSI", "470 AO-Volts": "0.0000", "PRIME-TransposeMirrorX": "0", "445 Shutter-SupportsTriggering": "0", "470 Shutter-SupportsTriggering": "0", "XLIGHT Hub-Description": "XLIGHT hub", "PRIME-PreampOffLimit": "10000", "470 AO-Sequenceable": "1", "528 Shutter-MaxVolts": "5.0000", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "Core-XYStage": "XYStage", "470 AO-TriggerSampleRate": "100000", "PRIME-Timing-PreTriggerDelayNs": "24672000.0000", "528-DA Device": "528 Shutter", "Focus-Description": "Nikon TE2000 focus adapter", "FilterBlock-State": "5", "520 AO-Demo": "No", "COM3-DelayBetweenCharsMs": "0.0000", "470 Shutter-Volts": "0.0000", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "PRIME-PP 2 THRESHOLD": "125", "PRIME-PP 4 THRESHOLD": "75", "XYStage-Acceleration": "4.0000", "470 Shutter-Demo": "No", "PRIME-Name": "PMPCIECam00", "640-DA Device": "640 Shutter", "405 AO-Block voltage": "false", "ElapsedTime-ms": 156623, "PRIME-Offset": "100", "445 AO-IOChannel": "Dev1/ao1", "640 AO-MaxVolts": "5.0000", "PositionIndex": 3, "PRIME-PP 1 ENABLED": "Yes", "COM1-BaudRate": "9600", "640-State": "1", "COM3-Verbose": "1", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "LudlController-TransmissionDelay": "0", "PRIME-FullWellCapacity": "62000", "PRIME-SMARTStreamingEnabled": "No", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "640 Shutter-Description": "NI DAC", "528-Description": "DA device that is used as a shutter", "COM4-Description": "Serial port driver (boost:asio)", "405 Shutter-Description": "NI DAC", "TE2000-Name": "TE2000", "528 Shutter-Description": "NI DAC", "COM9-Description": "Serial port driver (boost:asio)", "LudlController-Name": "LudlController", "445 Shutter-TriggerSequenceLength": "1000", "PRIME-SerialNumber": "A18B203008", "445 AO-Volts": "0.0000", "PRIME-X-dimension": "1200", "PRIME-ShutterCloseDelay": "0", "XLIGHT Disk slider-State": "2", "Position": "Default", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 AO-IOChannel": "Dev1/ao5", "520 AO-IOChannel": "Dev1/ao4", "528 AO-TriggerSequenceLength": "1000", "405 Shutter-MaxVolts": "5.0000", "470-DA Device": "470 Shutter", "PRIME-FTCapable": "No", "Core-ChannelGroup": "Channels", "555 AO-SupportsTriggering": "0", "PRIME-Y-dimension": "1200", "Frame": 0, "555 Shutter-Sequenceable": "1", "PRIME-Timing-ExposureTimeNs": "9992000.0000", "Core-ImageProcessor": "", "528-State": "0", "520 AO-Block voltage": "false", "XLIGHT Excitation Wheel-Label": "QUAD", "640 Shutter-Demo": "No", "COM3-StopBits": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "520 Shutter-IOChannel": "Dev1/ao11", "Width": 1200, "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-SupportsTriggering": "0", "640 AO-TriggerSampleRate": "100000", "520-Description": "DA device that is used as a shutter", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "405 Shutter-Sequenceable": "1", "405 Shutter-SupportsTriggering": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-TriggerSampleRate": "100000", "640 Shutter-MaxVolts": "5.0000", "555 AO-MaxVolts": "5.0000", "520 AO-TriggerSequenceLength": "1000", "COM3-BaudRate": "9600", "COM9-BaudRate": "9600", "Vincent-D3-Channel #": "Ch. #1", "PRIME-PMode": "Normal", "OpticalPath-Label": "Left(100)", "BitDepth": 16, "555 AO-TriggerSequenceLength": "1000", "445 Shutter-Description": "NI DAC", "405 AO-Sequenceable": "1", "PRIME-AcquisitionMethod": "Callbacks", "555 AO-Name": "AnalogIO", "PRIME-ShutterMode": "Pre-Exposure", "COM3-Handshaking": "Off", "445 AO-SupportsTriggering": "0", "ROI": "0-0-1200-1200", "COM9-Verbose": "1", "PRIME-Color": "OFF", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "PRIME-ShutterOpenDelay": "0", "COM1-Parity": "None", "Slice": 7, "SlicePosition": 20, "COM9-DelayBetweenCharsMs": "0.0000", "555-Name": "DA Shutter", "PIEZO-LudlSingleAxisName": "B", "XLIGHT Emission Wheel-Label": "725/40 (C)", "640 Shutter-Name": "AnalogIO", "640 AO-Sequenceable": "1", "520 Shutter-Demo": "No", "528 Shutter-Demo": "No", "470-Name": "DA Shutter", "520-DA Device": "520 Shutter", "XYStage-Description": "Ludl XY stage driver adapter", "445 Shutter-MinVolts": "0.0000", "528 AO-TriggerSampleRate": "100000", "405 AO-MinVolts": "0.0000", "405 Shutter-TriggerSequenceLength": "1000", "Height": 1200, "555 AO-Block voltage": "false", "640 Shutter-Volts": "5.0000", "PixelType": "GRAY16", "445 Shutter-Block voltage": "false", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "520 Shutter-TriggerSequenceLength": "1000", "COM9-Name": "COM9", "Core-SLM": "", "555 AO-Volts": "0.0000", "555 Shutter-Block voltage": "false", "XYStage-Speed": "1800.0000", "520 AO-MaxVolts": "5.0000", "528 Shutter-SupportsTriggering": "0", "COM1-Description": "Serial port driver (boost:asio)", "PRIME-MetadataEnabled": "No", "520 Shutter-MinVolts": "0.0000", "470 Shutter-Sequenceable": "1", "PRIME-PreampDelay": "0", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-MinVolts": "0.0000", "ChannelIndex": 3, "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Core-AutoShutter": "1", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "Exposure-ms": 520, "PRIME-PP 2 ENABLED": "Yes", "640 Shutter-TriggerSampleRate": "100000", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "470 Shutter-IOChannel": "Dev1/ao10", "405 AO-Demo": "No", "PixelSizeUm": 1.1, "470 AO-Demo": "No", "470 Shutter-MaxVolts": "5.0000", "Vincent-D3-Name": "Vincent-D3", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FileName": "img_000000000_Il-C_007.tif", "LudlController-Reset": "Operate", "PRIME-Exposure": "520.00", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "445-Name": "DA Shutter", "555 Shutter-Name": "AnalogIO", "520 Shutter-TriggerSampleRate": "100000", "445 Shutter-IOChannel": "Dev1/ao9", "PRIME-TransposeCorrection": "0", "XYStage-StepSize": "0.1000", "445-Description": "DA device that is used as a shutter", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 AO-IOChannel": "Dev1/ao7", "520 Shutter-Name": "AnalogIO", "COM3-DataBits": "8", "640 AO-Volts": "4.5000", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "555 Shutter-IOChannel": "Dev1/ao13", "WaitInterval": null, "520 Shutter-Sequenceable": "1", "YPositionUm": 20690.300000000003, "445-State": "0", "445-DA Device": "445 Shutter", "PRIME-CircularBufferAutoSize": "ON", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "555 AO-TriggerSampleRate": "100000", "445 Shutter-Volts": "0.0000", "XLIGHT Hub-Port": "COM3", "Core-AutoFocus": "", "COM4-Handshaking": "Off", "528 Shutter-MinVolts": "0.0000", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "445 Shutter-Name": "AnalogIO", "528 Shutter-Sequenceable": "1", "XYStage-TransposeMirrorX": "0", "XYStage-TransposeMirrorY": "0", "PRIME-ReadoutRate": "100MHz 16bit", "XYStage-Name": "Stage", "640 AO-SupportsTriggering": "0", "640-Name": "DA Shutter", "640 Shutter-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "640 AO-Block voltage": "false", "445 Shutter-Sequenceable": "1", "470 AO-MaxVolts": "5.0000", "COM4-BaudRate": "9600", "470-Description": "DA device that is used as a shutter", "520 Shutter-Description": "NI DAC", "PRIME-CCDTemperature": "-14.9600", "Time": "2019-12-17 21:59:04 -0500", "640 AO-TriggerSequenceLength": "1000", "470 AO-Name": "AnalogIO", "XLIGHT Disk slider-Label": "Disk Pos 40um", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "Binning": "1x1", "TE2000-ModelType": "1", "COM3-AnswerTimeout": "500.0000", "555 Shutter-SupportsTriggering": "0", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "TE2000-Port": "COM4", "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Description": "NI DAC", "COM4-AnswerTimeout": "500.0000", "COM1-DelayBetweenCharsMs": "0.0000", "FilterBlock-Label": "Confocal", "445 AO-Name": "AnalogIO", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-Timing-ReadoutTimeNs": "24672000.0000", "528 AO-Demo": "No", "520 AO-Sequenceable": "1", "555-Description": "DA device that is used as a shutter", "405-DA Device": "405 Shutter", "405 AO-TriggerSequenceLength": "1000", "640 AO-MinVolts": "0.0000", "640 Shutter-Block voltage": "false", "FrameIndex": 0, "PRIME-Port": "CMOS", "555 AO-MinVolts": "0.0000", "COM3-Description": "Serial port driver (boost:asio)", "528 AO-Description": "NI DAC", "555 AO-IOChannel": "Dev1/ao6", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "555 AO-TriggerInputLine": "/Dev1/PFI0", "PRIME-ActualInterval-ms": "374.3700", "LudlController-Port": "COM9", "445 Shutter-Demo": "No", "PRIME-Trigger-Expose Out-Mux": "1", "SliceIndex": 7, "PRIME-CCDTemperatureSetPoint": "-15.0000", "405 Shutter-MinVolts": "0.0000", "PRIME-ExposeOutMode": "First Row", "AxisPositions": null, "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Volts": "0.0000", "528-Name": "DA Shutter", "555-State": "0", "XLIGHT Dichroic-Label": "Di03-R635-t1", "445 AO-Sequenceable": "1", "405-Name": "DA Shutter", "405 AO-IOChannel": "Dev1/ao0", "Core-Camera": "PRIME", "Focus-Name": "Focus", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "405 Shutter-IOChannel": "Dev1/ao8", "640 AO-Demo": "No", "640 Shutter-SupportsTriggering": "0", "445 AO-Demo": "No", "555-DA Device": "555 Shutter", "470 Shutter-Description": "NI DAC", "528 AO-Volts": "0.0000", "COM1-DataBits": "8", "Vincent-D3-Address": "x", "520 AO-SupportsTriggering": "0", "405-Description": "DA device that is used as a shutter", "640 Shutter-MinVolts": "0.0000", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "405 AO-Name": "AnalogIO", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "640 AO-TriggerInputLine": "/Dev1/PFI0", "405 AO-TriggerInputLine": "/Dev1/PFI0", "640-Description": "DA device that is used as a shutter", "445 Shutter-TriggerSampleRate": "100000", "470 Shutter-TriggerSequenceLength": "1000", "COM1-StopBits": "1", "520 Shutter-SupportsTriggering": "0", "405-State": "0", "Core-Initialize": "1", "XLIGHT Emission Wheel-State": "7", "520 AO-Description": "NI DAC", "528 AO-TriggerInputLine": "/Dev1/PFI0", "445 Shutter-MaxVolts": "5.0000", "COM9-Handshaking": "Off", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "XLIGHT Spin motor-Label": "On (spinning)", "PRIME-PP 5 ENABLED": "No", "UUID": "79e14fd9-9041-462b-9a9f-cf3044f0d7bf", "PRIME-TriggerMode": "Internal Trigger", "COM1-Handshaking": "Off", "PRIME-Timing-PostTriggerDelayNs": "49344000.0000", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "Focus-Position": "16479.8000", "528 Shutter-Block voltage": "false", "Channel": "Il-C", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "555 Shutter-Demo": "No", "405 Shutter-Demo": "No", "528 AO-Name": "AnalogIO", "520-State": "0", "Core-Galvo": "", "405 Shutter-TriggerSampleRate": "100000", "Focus-FocusStepSize_(nm)": "100", "PRIME-PixelType": "16bit", "COM4-DataBits": "8", "470 AO-IOChannel": "Dev1/ao2", "520 AO-MinVolts": "0.0000", "COM9-Parity": "None", "PRIME-CircularBufferEnabled": "ON", "PRIME-PP 6 Reset": "No", "640 AO-Description": "NI DAC", "Source": "PRIME", "445 AO-MinVolts": "0.0000", "PIEZO-Name": "Stage", "445 AO-Description": "NI DAC", "PRIME-CircularBufferFrameCount": "46", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "520 AO-Volts": "0.0000", "528 Shutter-TriggerSampleRate": "100000", "XPositionUm": 62321, "OpticalPath-State": "4", "PRIME-PP 3 THRESHOLD": "80", "PRIME-PVCAM Version": "3.7.1", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Gain": "1-HDR", "640 Shutter-TriggerSequenceLength": "1000", "528 AO-SupportsTriggering": "0", "445 AO-TriggerInputLine": "/Dev1/PFI0", "OpticalPath-Name": "OpticalPath", "PRIME-PP 4 ENABLED": "Yes", "PRIME-PVCAM Adapter Version": "1.3.29", "COM3-Name": "COM3", "405 AO-MaxVolts": "5.0000", "520 Shutter-Block voltage": "false", "PRIME-Timing-ClearingTimeNs": "49344000.0000", "Core-Focus": "PIEZO", "528 AO-Block voltage": "false", "TE2000-Description": "Nikon TE2000 hub", "528 AO-MinVolts": "0.0000", "COM4-Parity": "None", "Summary": { "Slices": 11, "ChContrastMin": [ 0, 0, 0, 0, 0, 0 ], "PixelAspect": 1, "SlicesFirst": true, "Interval_ms": 0, "Time": "2019-12-17 21:58:39 -0500", "Prefix": "seq_3", "InitialPositionList": [ { "Label": "Pos0", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.435], "Focus": [16480.2], "XYStage": [ 69822.40000000001, 20100.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos1", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.425], "Focus": [16480.3], "XYStage": [ 69004, 20101 ] }, "GridColumnIndex": 0 }, { "Label": "Pos2", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.44], "Focus": [16480.1], "XYStage": [ 63119.5, 20690.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos3", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.405], "Focus": [16480.2], "XYStage": [ 62321, 20690.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos4", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.415], "Focus": [16480], "XYStage": [ 56604.100000000006, 20688.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos5", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.42000000000002], "Focus": [16480.1], "XYStage": [ 56034.200000000004, 20688.800000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos6", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.455], "Focus": [16480], "XYStage": [ 50889.3, 20496.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos7", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.43], "Focus": [16480.2], "XYStage": [ 50101.600000000006, 20496.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos8", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.67000000000002], "Focus": [16479.9], "XYStage": [ 44818.600000000006, 20496.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos9", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.67000000000002], "Focus": [16480], "XYStage": [ 43897.9, 20436.800000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos10", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.675], "Focus": [16479.9], "XYStage": [ 39670.600000000006, 20664.9 ] }, "GridColumnIndex": 0 }, { "Label": "Pos11", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.69], "Focus": [16479.9], "XYStage": [ 38734, 20664.600000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos12", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.695], "Focus": [16480.1], "XYStage": [ 34037.200000000004, 20664.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos13", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.685], "Focus": [16480.1], "XYStage": [ 33152, 20664.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos14", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.69], "Focus": [16480.1], "XYStage": [ 28122.800000000003, 20664.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos15", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.675], "Focus": [16480], "XYStage": [ 27422.9, 20664.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos16", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.64000000000001], "Focus": [16480], "XYStage": [ 23005.2, 20664.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos17", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.69], "Focus": [16479.9], "XYStage": [ 21820.600000000002, 20409.600000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos18", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.41], "Focus": [16480.1], "XYStage": [ 73697.6, -13222.800000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos19", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.455], "Focus": [16480.1], "XYStage": [ 72765.8, -13223 ] }, "GridColumnIndex": 0 }, { "Label": "Pos20", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.4], "Focus": [16480.2], "XYStage": [ 67445.5, -12303.800000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos21", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.415], "Focus": [16480.2], "XYStage": [ 66613.3, -12303.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos22", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.415], "Focus": [16480.1], "XYStage": [ 61819.4, -11936.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos23", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.405], "Focus": [16480.2], "XYStage": [ 61325.700000000004, -11937.1 ] }, "GridColumnIndex": 0 }, { "Label": "Pos24", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.46], "Focus": [16480.2], "XYStage": [ 55377, -10974.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos25", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.445], "Focus": [16480.1], "XYStage": [ 54827, -11214.800000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos26", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.435], "Focus": [16480.2], "XYStage": [ 49937.100000000006, -10919.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos27", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.425], "Focus": [16480.1], "XYStage": [ 48764, -10919.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos28", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.42000000000002], "Focus": [16480.2], "XYStage": [ 43978.4, -10920.400000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos29", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.435], "Focus": [16480.2], "XYStage": [ 43106.3, -10920.400000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos30", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.405], "Focus": [16480.3], "XYStage": [ 38212.4, -10920.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos31", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.445], "Focus": [16480.2], "XYStage": [ 37254.9, -10920.400000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos32", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.44], "Focus": [16480.3], "XYStage": [ 32472.800000000003, -11294.400000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos33", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [166.95000000000002], "Focus": [16480.2], "XYStage": [ 31420.7, -11294.400000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos34", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [166.95000000000002], "Focus": [16480.2], "XYStage": [ 26881.5, -11640 ] }, "GridColumnIndex": 0 }, { "Label": "Pos35", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [166.98], "Focus": [16480.2], "XYStage": [ 25876.100000000002, -11641.1 ] }, "GridColumnIndex": 0 } ], "z-step_um": 10, "GridRow": 0, "BitDepth": 16, "Depth": 2, "Channels": 6, "Source": "Micro-Manager", "ChColors": [ -16711681, -256, -65281, -1, -1, -16776961 ], "MetadataVersion": 10, "KeepShutterOpenChannels": true, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536, 65536 ], "CustomIntervals_ms": [], "Height": 1200, "MicroManagerVersion": "1.4.23 20180219", "GridColumn": 0, "UUID": "8c8f9d20-b128-4577-9f91-89e70f22d319", "PixelType": "GRAY16", "Width": 1200, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\Yan\\20191217-YC113-S5S6", "Frames": 1, "Comment": "", "UserName": "Nikon", "PixelSize_um": 1.1, "ROI": [ 0, 0, 1200, 1200 ], "Date": "2019-12-17", "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC", "P-DAPI-high" ], "KeepShutterOpenSlices": true, "IJType": 1, "ComputerName": "ZadorConfocal2", "Positions": 36, "PositionIndex": 3, "CameraTimeout": 20000, "TimeFirst": false }, "640 AO-Name": "AnalogIO", "Vincent-D3-Command": "Close" }, "FrameKey-0-3-8": { "COM4-StopBits": "1", "528 AO-MaxVolts": "5.0000", "XLIGHT Excitation Wheel-State": "2", "470 Shutter-Name": "AnalogIO", "Core-Shutter": "640", "555 AO-Demo": "No", "XLIGHT Dichroic-State": "2", "XYStage-StepSize-Y": "0.1000", "ZPositionUm": 207.405, "XYStage-StepSize-X": "0.1000", "PRIME-FirmwareVersion": "20.28", "PRIME-ClearCycles": "2", "520-Name": "DA Shutter", "405 Shutter-Name": "AnalogIO", "PIEZO-StepSize": "0.0050", "PRIME-ClearMode": "Pre-Exposure", "470 AO-MinVolts": "0.0000", "405 AO-SupportsTriggering": "1", "CameraChannelIndex": 0, "XYStage-StartSpeed": "500.0000", "COM9-StopBits": "2", "405 AO-Description": "NI DAC", "COM4-DelayBetweenCharsMs": "0.0000", "445 AO-Block voltage": "false", "520 AO-TriggerInputLine": "/Dev1/PFI0", "555 AO-Description": "NI DAC", "520 AO-TriggerSampleRate": "100000", "COM4-Verbose": "1", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM4-Name": "COM4", "405 Shutter-Volts": "0.0000", "Vincent-D3-Port": "COM1", "PRIME-PP 4 MIN ADU AFFECTED": "200", "470 Shutter-MinVolts": "0.0000", "PRIME-PP 3 ENABLED": "Yes", "470 AO-Description": "NI DAC", "PRIME-Description": "PVCAM API device adapter", "520 Shutter-MaxVolts": "5.0000", "520 Shutter-Volts": "0.0000", "528 AO-Sequenceable": "1", "PRIME-Binning": "1x1", "555 Shutter-MaxVolts": "5.0000", "528 Shutter-TriggerSequenceLength": "1000", "405 Shutter-Block voltage": "false", "XLIGHT Spin motor-State": "1", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "Vincent-D3-Time to open (ms)": "35.0000", "Vincent-D3-Time to close (ms)": "35.0000", "445 AO-TriggerSampleRate": "100000", "640 Shutter-IOChannel": "Dev1/ao14", "555 AO-Sequenceable": "1", "520 AO-Name": "AnalogIO", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "470 AO-Block voltage": "false", "COM1-Verbose": "1", "COM9-AnswerTimeout": "10000.0000", "528 Shutter-Name": "AnalogIO", "PRIME-Trigger Timeout (secs)": "10", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "NextFrame": 0, "470 AO-TriggerSequenceLength": "1000", "PRIME-TransposeXY": "0", "445 AO-TriggerSequenceLength": "1000", "COM1-Name": "COM1", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "COM9-DataBits": "8", "COM1-AnswerTimeout": "500.0000", "Camera": "", "TE2000-Version": "V2.50-V2.50-V2.10", "528 Shutter-Volts": "0.0000", "XLIGHT Hub-Name": "XLIGHT Hub", "PIEZO-Description": "Ludl stage driver adapter", "PositionName": "Pos3", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSampleRate": "100000", "PRIME-FanSpeedSetpoint": "High", "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "405 AO-TriggerSampleRate": "100000", "470 Shutter-Block voltage": "false", "470-State": "0", "COM3-Parity": "None", "405 AO-Volts": "0.0000", "PRIME-TransposeMirrorY": "0", "PRIME-ChipName": "GS144BSI", "470 AO-Volts": "0.0000", "PRIME-TransposeMirrorX": "0", "445 Shutter-SupportsTriggering": "0", "470 Shutter-SupportsTriggering": "0", "XLIGHT Hub-Description": "XLIGHT hub", "PRIME-PreampOffLimit": "10000", "470 AO-Sequenceable": "1", "528 Shutter-MaxVolts": "5.0000", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "Core-XYStage": "XYStage", "470 AO-TriggerSampleRate": "100000", "PRIME-Timing-PreTriggerDelayNs": "24672000.0000", "528-DA Device": "528 Shutter", "Focus-Description": "Nikon TE2000 focus adapter", "FilterBlock-State": "5", "520 AO-Demo": "No", "COM3-DelayBetweenCharsMs": "0.0000", "470 Shutter-Volts": "0.0000", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "PRIME-PP 2 THRESHOLD": "125", "PRIME-PP 4 THRESHOLD": "75", "XYStage-Acceleration": "4.0000", "470 Shutter-Demo": "No", "PRIME-Name": "PMPCIECam00", "640-DA Device": "640 Shutter", "405 AO-Block voltage": "false", "ElapsedTime-ms": 157374, "PRIME-Offset": "100", "445 AO-IOChannel": "Dev1/ao1", "640 AO-MaxVolts": "5.0000", "PositionIndex": 3, "PRIME-PP 1 ENABLED": "Yes", "COM1-BaudRate": "9600", "640-State": "1", "COM3-Verbose": "1", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "LudlController-TransmissionDelay": "0", "PRIME-FullWellCapacity": "62000", "PRIME-SMARTStreamingEnabled": "No", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "640 Shutter-Description": "NI DAC", "528-Description": "DA device that is used as a shutter", "COM4-Description": "Serial port driver (boost:asio)", "405 Shutter-Description": "NI DAC", "TE2000-Name": "TE2000", "528 Shutter-Description": "NI DAC", "COM9-Description": "Serial port driver (boost:asio)", "LudlController-Name": "LudlController", "445 Shutter-TriggerSequenceLength": "1000", "PRIME-SerialNumber": "A18B203008", "445 AO-Volts": "0.0000", "PRIME-X-dimension": "1200", "PRIME-ShutterCloseDelay": "0", "XLIGHT Disk slider-State": "2", "Position": "Default", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 AO-IOChannel": "Dev1/ao5", "520 AO-IOChannel": "Dev1/ao4", "528 AO-TriggerSequenceLength": "1000", "405 Shutter-MaxVolts": "5.0000", "470-DA Device": "470 Shutter", "PRIME-FTCapable": "No", "Core-ChannelGroup": "Channels", "555 AO-SupportsTriggering": "0", "PRIME-Y-dimension": "1200", "Frame": 0, "555 Shutter-Sequenceable": "1", "PRIME-Timing-ExposureTimeNs": "9992000.0000", "Core-ImageProcessor": "", "528-State": "0", "520 AO-Block voltage": "false", "XLIGHT Excitation Wheel-Label": "QUAD", "640 Shutter-Demo": "No", "COM3-StopBits": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "520 Shutter-IOChannel": "Dev1/ao11", "Width": 1200, "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-SupportsTriggering": "0", "640 AO-TriggerSampleRate": "100000", "520-Description": "DA device that is used as a shutter", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "405 Shutter-Sequenceable": "1", "405 Shutter-SupportsTriggering": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-TriggerSampleRate": "100000", "640 Shutter-MaxVolts": "5.0000", "555 AO-MaxVolts": "5.0000", "520 AO-TriggerSequenceLength": "1000", "COM3-BaudRate": "9600", "COM9-BaudRate": "9600", "Vincent-D3-Channel #": "Ch. #1", "PRIME-PMode": "Normal", "OpticalPath-Label": "Left(100)", "BitDepth": 16, "555 AO-TriggerSequenceLength": "1000", "445 Shutter-Description": "NI DAC", "405 AO-Sequenceable": "1", "PRIME-AcquisitionMethod": "Callbacks", "555 AO-Name": "AnalogIO", "PRIME-ShutterMode": "Pre-Exposure", "COM3-Handshaking": "Off", "445 AO-SupportsTriggering": "0", "ROI": "0-0-1200-1200", "COM9-Verbose": "1", "PRIME-Color": "OFF", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "PRIME-ShutterOpenDelay": "0", "COM1-Parity": "None", "Slice": 8, "SlicePosition": 30, "COM9-DelayBetweenCharsMs": "0.0000", "555-Name": "DA Shutter", "PIEZO-LudlSingleAxisName": "B", "XLIGHT Emission Wheel-Label": "725/40 (C)", "640 Shutter-Name": "AnalogIO", "640 AO-Sequenceable": "1", "520 Shutter-Demo": "No", "528 Shutter-Demo": "No", "470-Name": "DA Shutter", "520-DA Device": "520 Shutter", "XYStage-Description": "Ludl XY stage driver adapter", "445 Shutter-MinVolts": "0.0000", "528 AO-TriggerSampleRate": "100000", "405 AO-MinVolts": "0.0000", "405 Shutter-TriggerSequenceLength": "1000", "Height": 1200, "555 AO-Block voltage": "false", "640 Shutter-Volts": "5.0000", "PixelType": "GRAY16", "445 Shutter-Block voltage": "false", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "520 Shutter-TriggerSequenceLength": "1000", "COM9-Name": "COM9", "Core-SLM": "", "555 AO-Volts": "0.0000", "555 Shutter-Block voltage": "false", "XYStage-Speed": "1800.0000", "520 AO-MaxVolts": "5.0000", "528 Shutter-SupportsTriggering": "0", "COM1-Description": "Serial port driver (boost:asio)", "PRIME-MetadataEnabled": "No", "520 Shutter-MinVolts": "0.0000", "470 Shutter-Sequenceable": "1", "PRIME-PreampDelay": "0", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-MinVolts": "0.0000", "ChannelIndex": 3, "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Core-AutoShutter": "1", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "Exposure-ms": 520, "PRIME-PP 2 ENABLED": "Yes", "640 Shutter-TriggerSampleRate": "100000", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "470 Shutter-IOChannel": "Dev1/ao10", "405 AO-Demo": "No", "PixelSizeUm": 1.1, "470 AO-Demo": "No", "470 Shutter-MaxVolts": "5.0000", "Vincent-D3-Name": "Vincent-D3", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FileName": "img_000000000_Il-C_008.tif", "LudlController-Reset": "Operate", "PRIME-Exposure": "520.00", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "445-Name": "DA Shutter", "555 Shutter-Name": "AnalogIO", "520 Shutter-TriggerSampleRate": "100000", "445 Shutter-IOChannel": "Dev1/ao9", "PRIME-TransposeCorrection": "0", "XYStage-StepSize": "0.1000", "445-Description": "DA device that is used as a shutter", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 AO-IOChannel": "Dev1/ao7", "520 Shutter-Name": "AnalogIO", "COM3-DataBits": "8", "640 AO-Volts": "4.5000", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "555 Shutter-IOChannel": "Dev1/ao13", "WaitInterval": null, "520 Shutter-Sequenceable": "1", "YPositionUm": 20690.300000000003, "445-State": "0", "445-DA Device": "445 Shutter", "PRIME-CircularBufferAutoSize": "ON", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "555 AO-TriggerSampleRate": "100000", "445 Shutter-Volts": "0.0000", "XLIGHT Hub-Port": "COM3", "Core-AutoFocus": "", "COM4-Handshaking": "Off", "528 Shutter-MinVolts": "0.0000", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "445 Shutter-Name": "AnalogIO", "528 Shutter-Sequenceable": "1", "XYStage-TransposeMirrorX": "0", "XYStage-TransposeMirrorY": "0", "PRIME-ReadoutRate": "100MHz 16bit", "XYStage-Name": "Stage", "640 AO-SupportsTriggering": "0", "640-Name": "DA Shutter", "640 Shutter-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "640 AO-Block voltage": "false", "445 Shutter-Sequenceable": "1", "470 AO-MaxVolts": "5.0000", "COM4-BaudRate": "9600", "470-Description": "DA device that is used as a shutter", "520 Shutter-Description": "NI DAC", "PRIME-CCDTemperature": "-14.9600", "Time": "2019-12-17 21:59:04 -0500", "640 AO-TriggerSequenceLength": "1000", "470 AO-Name": "AnalogIO", "XLIGHT Disk slider-Label": "Disk Pos 40um", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "Binning": "1x1", "TE2000-ModelType": "1", "COM3-AnswerTimeout": "500.0000", "555 Shutter-SupportsTriggering": "0", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "TE2000-Port": "COM4", "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Description": "NI DAC", "COM4-AnswerTimeout": "500.0000", "COM1-DelayBetweenCharsMs": "0.0000", "FilterBlock-Label": "Confocal", "445 AO-Name": "AnalogIO", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-Timing-ReadoutTimeNs": "24672000.0000", "528 AO-Demo": "No", "520 AO-Sequenceable": "1", "555-Description": "DA device that is used as a shutter", "405-DA Device": "405 Shutter", "405 AO-TriggerSequenceLength": "1000", "640 AO-MinVolts": "0.0000", "640 Shutter-Block voltage": "false", "FrameIndex": 0, "PRIME-Port": "CMOS", "555 AO-MinVolts": "0.0000", "COM3-Description": "Serial port driver (boost:asio)", "528 AO-Description": "NI DAC", "555 AO-IOChannel": "Dev1/ao6", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "555 AO-TriggerInputLine": "/Dev1/PFI0", "PRIME-ActualInterval-ms": "374.3700", "LudlController-Port": "COM9", "445 Shutter-Demo": "No", "PRIME-Trigger-Expose Out-Mux": "1", "SliceIndex": 8, "PRIME-CCDTemperatureSetPoint": "-15.0000", "405 Shutter-MinVolts": "0.0000", "PRIME-ExposeOutMode": "First Row", "AxisPositions": null, "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Volts": "0.0000", "528-Name": "DA Shutter", "555-State": "0", "XLIGHT Dichroic-Label": "Di03-R635-t1", "445 AO-Sequenceable": "1", "405-Name": "DA Shutter", "405 AO-IOChannel": "Dev1/ao0", "Core-Camera": "PRIME", "Focus-Name": "Focus", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "405 Shutter-IOChannel": "Dev1/ao8", "640 AO-Demo": "No", "640 Shutter-SupportsTriggering": "0", "445 AO-Demo": "No", "555-DA Device": "555 Shutter", "470 Shutter-Description": "NI DAC", "528 AO-Volts": "0.0000", "COM1-DataBits": "8", "Vincent-D3-Address": "x", "520 AO-SupportsTriggering": "0", "405-Description": "DA device that is used as a shutter", "640 Shutter-MinVolts": "0.0000", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "405 AO-Name": "AnalogIO", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "640 AO-TriggerInputLine": "/Dev1/PFI0", "405 AO-TriggerInputLine": "/Dev1/PFI0", "640-Description": "DA device that is used as a shutter", "445 Shutter-TriggerSampleRate": "100000", "470 Shutter-TriggerSequenceLength": "1000", "COM1-StopBits": "1", "520 Shutter-SupportsTriggering": "0", "405-State": "0", "Core-Initialize": "1", "XLIGHT Emission Wheel-State": "7", "520 AO-Description": "NI DAC", "528 AO-TriggerInputLine": "/Dev1/PFI0", "445 Shutter-MaxVolts": "5.0000", "COM9-Handshaking": "Off", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "XLIGHT Spin motor-Label": "On (spinning)", "PRIME-PP 5 ENABLED": "No", "UUID": "6038035b-e7cb-4b5b-ab8f-e6a32e168325", "PRIME-TriggerMode": "Internal Trigger", "COM1-Handshaking": "Off", "PRIME-Timing-PostTriggerDelayNs": "49344000.0000", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "Focus-Position": "16479.8000", "528 Shutter-Block voltage": "false", "Channel": "Il-C", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "555 Shutter-Demo": "No", "405 Shutter-Demo": "No", "528 AO-Name": "AnalogIO", "520-State": "0", "Core-Galvo": "", "405 Shutter-TriggerSampleRate": "100000", "Focus-FocusStepSize_(nm)": "100", "PRIME-PixelType": "16bit", "COM4-DataBits": "8", "470 AO-IOChannel": "Dev1/ao2", "520 AO-MinVolts": "0.0000", "COM9-Parity": "None", "PRIME-CircularBufferEnabled": "ON", "PRIME-PP 6 Reset": "No", "640 AO-Description": "NI DAC", "Source": "PRIME", "445 AO-MinVolts": "0.0000", "PIEZO-Name": "Stage", "445 AO-Description": "NI DAC", "PRIME-CircularBufferFrameCount": "46", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "520 AO-Volts": "0.0000", "528 Shutter-TriggerSampleRate": "100000", "XPositionUm": 62321, "OpticalPath-State": "4", "PRIME-PP 3 THRESHOLD": "80", "PRIME-PVCAM Version": "3.7.1", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Gain": "1-HDR", "640 Shutter-TriggerSequenceLength": "1000", "528 AO-SupportsTriggering": "0", "445 AO-TriggerInputLine": "/Dev1/PFI0", "OpticalPath-Name": "OpticalPath", "PRIME-PP 4 ENABLED": "Yes", "PRIME-PVCAM Adapter Version": "1.3.29", "COM3-Name": "COM3", "405 AO-MaxVolts": "5.0000", "520 Shutter-Block voltage": "false", "PRIME-Timing-ClearingTimeNs": "49344000.0000", "Core-Focus": "PIEZO", "528 AO-Block voltage": "false", "TE2000-Description": "Nikon TE2000 hub", "528 AO-MinVolts": "0.0000", "COM4-Parity": "None", "Summary": { "Slices": 11, "ChContrastMin": [ 0, 0, 0, 0, 0, 0 ], "PixelAspect": 1, "SlicesFirst": true, "Interval_ms": 0, "Time": "2019-12-17 21:58:39 -0500", "Prefix": "seq_3", "InitialPositionList": [ { "Label": "Pos0", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.435], "Focus": [16480.2], "XYStage": [ 69822.40000000001, 20100.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos1", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.425], "Focus": [16480.3], "XYStage": [ 69004, 20101 ] }, "GridColumnIndex": 0 }, { "Label": "Pos2", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.44], "Focus": [16480.1], "XYStage": [ 63119.5, 20690.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos3", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.405], "Focus": [16480.2], "XYStage": [ 62321, 20690.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos4", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.415], "Focus": [16480], "XYStage": [ 56604.100000000006, 20688.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos5", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.42000000000002], "Focus": [16480.1], "XYStage": [ 56034.200000000004, 20688.800000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos6", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.455], "Focus": [16480], "XYStage": [ 50889.3, 20496.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos7", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.43], "Focus": [16480.2], "XYStage": [ 50101.600000000006, 20496.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos8", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.67000000000002], "Focus": [16479.9], "XYStage": [ 44818.600000000006, 20496.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos9", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.67000000000002], "Focus": [16480], "XYStage": [ 43897.9, 20436.800000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos10", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.675], "Focus": [16479.9], "XYStage": [ 39670.600000000006, 20664.9 ] }, "GridColumnIndex": 0 }, { "Label": "Pos11", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.69], "Focus": [16479.9], "XYStage": [ 38734, 20664.600000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos12", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.695], "Focus": [16480.1], "XYStage": [ 34037.200000000004, 20664.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos13", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.685], "Focus": [16480.1], "XYStage": [ 33152, 20664.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos14", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.69], "Focus": [16480.1], "XYStage": [ 28122.800000000003, 20664.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos15", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.675], "Focus": [16480], "XYStage": [ 27422.9, 20664.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos16", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.64000000000001], "Focus": [16480], "XYStage": [ 23005.2, 20664.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos17", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.69], "Focus": [16479.9], "XYStage": [ 21820.600000000002, 20409.600000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos18", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.41], "Focus": [16480.1], "XYStage": [ 73697.6, -13222.800000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos19", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.455], "Focus": [16480.1], "XYStage": [ 72765.8, -13223 ] }, "GridColumnIndex": 0 }, { "Label": "Pos20", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.4], "Focus": [16480.2], "XYStage": [ 67445.5, -12303.800000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos21", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.415], "Focus": [16480.2], "XYStage": [ 66613.3, -12303.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos22", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.415], "Focus": [16480.1], "XYStage": [ 61819.4, -11936.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos23", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.405], "Focus": [16480.2], "XYStage": [ 61325.700000000004, -11937.1 ] }, "GridColumnIndex": 0 }, { "Label": "Pos24", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.46], "Focus": [16480.2], "XYStage": [ 55377, -10974.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos25", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.445], "Focus": [16480.1], "XYStage": [ 54827, -11214.800000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos26", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.435], "Focus": [16480.2], "XYStage": [ 49937.100000000006, -10919.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos27", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.425], "Focus": [16480.1], "XYStage": [ 48764, -10919.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos28", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.42000000000002], "Focus": [16480.2], "XYStage": [ 43978.4, -10920.400000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos29", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.435], "Focus": [16480.2], "XYStage": [ 43106.3, -10920.400000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos30", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.405], "Focus": [16480.3], "XYStage": [ 38212.4, -10920.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos31", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.445], "Focus": [16480.2], "XYStage": [ 37254.9, -10920.400000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos32", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.44], "Focus": [16480.3], "XYStage": [ 32472.800000000003, -11294.400000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos33", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [166.95000000000002], "Focus": [16480.2], "XYStage": [ 31420.7, -11294.400000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos34", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [166.95000000000002], "Focus": [16480.2], "XYStage": [ 26881.5, -11640 ] }, "GridColumnIndex": 0 }, { "Label": "Pos35", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [166.98], "Focus": [16480.2], "XYStage": [ 25876.100000000002, -11641.1 ] }, "GridColumnIndex": 0 } ], "z-step_um": 10, "GridRow": 0, "BitDepth": 16, "Depth": 2, "Channels": 6, "Source": "Micro-Manager", "ChColors": [ -16711681, -256, -65281, -1, -1, -16776961 ], "MetadataVersion": 10, "KeepShutterOpenChannels": true, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536, 65536 ], "CustomIntervals_ms": [], "Height": 1200, "MicroManagerVersion": "1.4.23 20180219", "GridColumn": 0, "UUID": "8c8f9d20-b128-4577-9f91-89e70f22d319", "PixelType": "GRAY16", "Width": 1200, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\Yan\\20191217-YC113-S5S6", "Frames": 1, "Comment": "", "UserName": "Nikon", "PixelSize_um": 1.1, "ROI": [ 0, 0, 1200, 1200 ], "Date": "2019-12-17", "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC", "P-DAPI-high" ], "KeepShutterOpenSlices": true, "IJType": 1, "ComputerName": "ZadorConfocal2", "Positions": 36, "PositionIndex": 3, "CameraTimeout": 20000, "TimeFirst": false }, "640 AO-Name": "AnalogIO", "Vincent-D3-Command": "Close" }, "FrameKey-0-3-9": { "COM4-StopBits": "1", "528 AO-MaxVolts": "5.0000", "XLIGHT Excitation Wheel-State": "2", "470 Shutter-Name": "AnalogIO", "Core-Shutter": "640", "555 AO-Demo": "No", "XLIGHT Dichroic-State": "2", "XYStage-StepSize-Y": "0.1000", "ZPositionUm": 217.405, "XYStage-StepSize-X": "0.1000", "PRIME-FirmwareVersion": "20.28", "PRIME-ClearCycles": "2", "520-Name": "DA Shutter", "405 Shutter-Name": "AnalogIO", "PIEZO-StepSize": "0.0050", "PRIME-ClearMode": "Pre-Exposure", "470 AO-MinVolts": "0.0000", "405 AO-SupportsTriggering": "1", "CameraChannelIndex": 0, "XYStage-StartSpeed": "500.0000", "COM9-StopBits": "2", "405 AO-Description": "NI DAC", "COM4-DelayBetweenCharsMs": "0.0000", "445 AO-Block voltage": "false", "520 AO-TriggerInputLine": "/Dev1/PFI0", "555 AO-Description": "NI DAC", "520 AO-TriggerSampleRate": "100000", "COM4-Verbose": "1", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM4-Name": "COM4", "405 Shutter-Volts": "0.0000", "Vincent-D3-Port": "COM1", "PRIME-PP 4 MIN ADU AFFECTED": "200", "470 Shutter-MinVolts": "0.0000", "PRIME-PP 3 ENABLED": "Yes", "470 AO-Description": "NI DAC", "PRIME-Description": "PVCAM API device adapter", "520 Shutter-MaxVolts": "5.0000", "520 Shutter-Volts": "0.0000", "528 AO-Sequenceable": "1", "PRIME-Binning": "1x1", "555 Shutter-MaxVolts": "5.0000", "528 Shutter-TriggerSequenceLength": "1000", "405 Shutter-Block voltage": "false", "XLIGHT Spin motor-State": "1", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "Vincent-D3-Time to open (ms)": "35.0000", "Vincent-D3-Time to close (ms)": "35.0000", "445 AO-TriggerSampleRate": "100000", "640 Shutter-IOChannel": "Dev1/ao14", "555 AO-Sequenceable": "1", "520 AO-Name": "AnalogIO", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "470 AO-Block voltage": "false", "COM1-Verbose": "1", "COM9-AnswerTimeout": "10000.0000", "528 Shutter-Name": "AnalogIO", "PRIME-Trigger Timeout (secs)": "10", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "NextFrame": 0, "470 AO-TriggerSequenceLength": "1000", "PRIME-TransposeXY": "0", "445 AO-TriggerSequenceLength": "1000", "COM1-Name": "COM1", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "COM9-DataBits": "8", "COM1-AnswerTimeout": "500.0000", "Camera": "", "TE2000-Version": "V2.50-V2.50-V2.10", "528 Shutter-Volts": "0.0000", "XLIGHT Hub-Name": "XLIGHT Hub", "PIEZO-Description": "Ludl stage driver adapter", "PositionName": "Pos3", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSampleRate": "100000", "PRIME-FanSpeedSetpoint": "High", "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "405 AO-TriggerSampleRate": "100000", "470 Shutter-Block voltage": "false", "470-State": "0", "COM3-Parity": "None", "405 AO-Volts": "0.0000", "PRIME-TransposeMirrorY": "0", "PRIME-ChipName": "GS144BSI", "470 AO-Volts": "0.0000", "PRIME-TransposeMirrorX": "0", "445 Shutter-SupportsTriggering": "0", "470 Shutter-SupportsTriggering": "0", "XLIGHT Hub-Description": "XLIGHT hub", "PRIME-PreampOffLimit": "10000", "470 AO-Sequenceable": "1", "528 Shutter-MaxVolts": "5.0000", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "Core-XYStage": "XYStage", "470 AO-TriggerSampleRate": "100000", "PRIME-Timing-PreTriggerDelayNs": "24672000.0000", "528-DA Device": "528 Shutter", "Focus-Description": "Nikon TE2000 focus adapter", "FilterBlock-State": "5", "520 AO-Demo": "No", "COM3-DelayBetweenCharsMs": "0.0000", "470 Shutter-Volts": "0.0000", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "PRIME-PP 2 THRESHOLD": "125", "PRIME-PP 4 THRESHOLD": "75", "XYStage-Acceleration": "4.0000", "470 Shutter-Demo": "No", "PRIME-Name": "PMPCIECam00", "640-DA Device": "640 Shutter", "405 AO-Block voltage": "false", "ElapsedTime-ms": 158142, "PRIME-Offset": "100", "445 AO-IOChannel": "Dev1/ao1", "640 AO-MaxVolts": "5.0000", "PositionIndex": 3, "PRIME-PP 1 ENABLED": "Yes", "COM1-BaudRate": "9600", "640-State": "1", "COM3-Verbose": "1", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "LudlController-TransmissionDelay": "0", "PRIME-FullWellCapacity": "62000", "PRIME-SMARTStreamingEnabled": "No", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "640 Shutter-Description": "NI DAC", "528-Description": "DA device that is used as a shutter", "COM4-Description": "Serial port driver (boost:asio)", "405 Shutter-Description": "NI DAC", "TE2000-Name": "TE2000", "528 Shutter-Description": "NI DAC", "COM9-Description": "Serial port driver (boost:asio)", "LudlController-Name": "LudlController", "445 Shutter-TriggerSequenceLength": "1000", "PRIME-SerialNumber": "A18B203008", "445 AO-Volts": "0.0000", "PRIME-X-dimension": "1200", "PRIME-ShutterCloseDelay": "0", "XLIGHT Disk slider-State": "2", "Position": "Default", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 AO-IOChannel": "Dev1/ao5", "520 AO-IOChannel": "Dev1/ao4", "528 AO-TriggerSequenceLength": "1000", "405 Shutter-MaxVolts": "5.0000", "470-DA Device": "470 Shutter", "PRIME-FTCapable": "No", "Core-ChannelGroup": "Channels", "555 AO-SupportsTriggering": "0", "PRIME-Y-dimension": "1200", "Frame": 0, "555 Shutter-Sequenceable": "1", "PRIME-Timing-ExposureTimeNs": "9992000.0000", "Core-ImageProcessor": "", "528-State": "0", "520 AO-Block voltage": "false", "XLIGHT Excitation Wheel-Label": "QUAD", "640 Shutter-Demo": "No", "COM3-StopBits": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "520 Shutter-IOChannel": "Dev1/ao11", "Width": 1200, "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-SupportsTriggering": "0", "640 AO-TriggerSampleRate": "100000", "520-Description": "DA device that is used as a shutter", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "405 Shutter-Sequenceable": "1", "405 Shutter-SupportsTriggering": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-TriggerSampleRate": "100000", "640 Shutter-MaxVolts": "5.0000", "555 AO-MaxVolts": "5.0000", "520 AO-TriggerSequenceLength": "1000", "COM3-BaudRate": "9600", "COM9-BaudRate": "9600", "Vincent-D3-Channel #": "Ch. #1", "PRIME-PMode": "Normal", "OpticalPath-Label": "Left(100)", "BitDepth": 16, "555 AO-TriggerSequenceLength": "1000", "445 Shutter-Description": "NI DAC", "405 AO-Sequenceable": "1", "PRIME-AcquisitionMethod": "Callbacks", "555 AO-Name": "AnalogIO", "PRIME-ShutterMode": "Pre-Exposure", "COM3-Handshaking": "Off", "445 AO-SupportsTriggering": "0", "ROI": "0-0-1200-1200", "COM9-Verbose": "1", "PRIME-Color": "OFF", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "PRIME-ShutterOpenDelay": "0", "COM1-Parity": "None", "Slice": 9, "SlicePosition": 40, "COM9-DelayBetweenCharsMs": "0.0000", "555-Name": "DA Shutter", "PIEZO-LudlSingleAxisName": "B", "XLIGHT Emission Wheel-Label": "725/40 (C)", "640 Shutter-Name": "AnalogIO", "640 AO-Sequenceable": "1", "520 Shutter-Demo": "No", "528 Shutter-Demo": "No", "470-Name": "DA Shutter", "520-DA Device": "520 Shutter", "XYStage-Description": "Ludl XY stage driver adapter", "445 Shutter-MinVolts": "0.0000", "528 AO-TriggerSampleRate": "100000", "405 AO-MinVolts": "0.0000", "405 Shutter-TriggerSequenceLength": "1000", "Height": 1200, "555 AO-Block voltage": "false", "640 Shutter-Volts": "5.0000", "PixelType": "GRAY16", "445 Shutter-Block voltage": "false", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "520 Shutter-TriggerSequenceLength": "1000", "COM9-Name": "COM9", "Core-SLM": "", "555 AO-Volts": "0.0000", "555 Shutter-Block voltage": "false", "XYStage-Speed": "1800.0000", "520 AO-MaxVolts": "5.0000", "528 Shutter-SupportsTriggering": "0", "COM1-Description": "Serial port driver (boost:asio)", "PRIME-MetadataEnabled": "No", "520 Shutter-MinVolts": "0.0000", "470 Shutter-Sequenceable": "1", "PRIME-PreampDelay": "0", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-MinVolts": "0.0000", "ChannelIndex": 3, "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Core-AutoShutter": "1", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "Exposure-ms": 520, "PRIME-PP 2 ENABLED": "Yes", "640 Shutter-TriggerSampleRate": "100000", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "470 Shutter-IOChannel": "Dev1/ao10", "405 AO-Demo": "No", "PixelSizeUm": 1.1, "470 AO-Demo": "No", "470 Shutter-MaxVolts": "5.0000", "Vincent-D3-Name": "Vincent-D3", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FileName": "img_000000000_Il-C_009.tif", "LudlController-Reset": "Operate", "PRIME-Exposure": "520.00", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "445-Name": "DA Shutter", "555 Shutter-Name": "AnalogIO", "520 Shutter-TriggerSampleRate": "100000", "445 Shutter-IOChannel": "Dev1/ao9", "PRIME-TransposeCorrection": "0", "XYStage-StepSize": "0.1000", "445-Description": "DA device that is used as a shutter", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 AO-IOChannel": "Dev1/ao7", "520 Shutter-Name": "AnalogIO", "COM3-DataBits": "8", "640 AO-Volts": "4.5000", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "555 Shutter-IOChannel": "Dev1/ao13", "WaitInterval": null, "520 Shutter-Sequenceable": "1", "YPositionUm": 20690.300000000003, "445-State": "0", "445-DA Device": "445 Shutter", "PRIME-CircularBufferAutoSize": "ON", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "555 AO-TriggerSampleRate": "100000", "445 Shutter-Volts": "0.0000", "XLIGHT Hub-Port": "COM3", "Core-AutoFocus": "", "COM4-Handshaking": "Off", "528 Shutter-MinVolts": "0.0000", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "445 Shutter-Name": "AnalogIO", "528 Shutter-Sequenceable": "1", "XYStage-TransposeMirrorX": "0", "XYStage-TransposeMirrorY": "0", "PRIME-ReadoutRate": "100MHz 16bit", "XYStage-Name": "Stage", "640 AO-SupportsTriggering": "0", "640-Name": "DA Shutter", "640 Shutter-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "640 AO-Block voltage": "false", "445 Shutter-Sequenceable": "1", "470 AO-MaxVolts": "5.0000", "COM4-BaudRate": "9600", "470-Description": "DA device that is used as a shutter", "520 Shutter-Description": "NI DAC", "PRIME-CCDTemperature": "-14.9600", "Time": "2019-12-17 21:59:05 -0500", "640 AO-TriggerSequenceLength": "1000", "470 AO-Name": "AnalogIO", "XLIGHT Disk slider-Label": "Disk Pos 40um", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "Binning": "1x1", "TE2000-ModelType": "1", "COM3-AnswerTimeout": "500.0000", "555 Shutter-SupportsTriggering": "0", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "TE2000-Port": "COM4", "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Description": "NI DAC", "COM4-AnswerTimeout": "500.0000", "COM1-DelayBetweenCharsMs": "0.0000", "FilterBlock-Label": "Confocal", "445 AO-Name": "AnalogIO", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-Timing-ReadoutTimeNs": "24672000.0000", "528 AO-Demo": "No", "520 AO-Sequenceable": "1", "555-Description": "DA device that is used as a shutter", "405-DA Device": "405 Shutter", "405 AO-TriggerSequenceLength": "1000", "640 AO-MinVolts": "0.0000", "640 Shutter-Block voltage": "false", "FrameIndex": 0, "PRIME-Port": "CMOS", "555 AO-MinVolts": "0.0000", "COM3-Description": "Serial port driver (boost:asio)", "528 AO-Description": "NI DAC", "555 AO-IOChannel": "Dev1/ao6", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "555 AO-TriggerInputLine": "/Dev1/PFI0", "PRIME-ActualInterval-ms": "374.3700", "LudlController-Port": "COM9", "445 Shutter-Demo": "No", "PRIME-Trigger-Expose Out-Mux": "1", "SliceIndex": 9, "PRIME-CCDTemperatureSetPoint": "-15.0000", "405 Shutter-MinVolts": "0.0000", "PRIME-ExposeOutMode": "First Row", "AxisPositions": null, "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Volts": "0.0000", "528-Name": "DA Shutter", "555-State": "0", "XLIGHT Dichroic-Label": "Di03-R635-t1", "445 AO-Sequenceable": "1", "405-Name": "DA Shutter", "405 AO-IOChannel": "Dev1/ao0", "Core-Camera": "PRIME", "Focus-Name": "Focus", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "405 Shutter-IOChannel": "Dev1/ao8", "640 AO-Demo": "No", "640 Shutter-SupportsTriggering": "0", "445 AO-Demo": "No", "555-DA Device": "555 Shutter", "470 Shutter-Description": "NI DAC", "528 AO-Volts": "0.0000", "COM1-DataBits": "8", "Vincent-D3-Address": "x", "520 AO-SupportsTriggering": "0", "405-Description": "DA device that is used as a shutter", "640 Shutter-MinVolts": "0.0000", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "405 AO-Name": "AnalogIO", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "640 AO-TriggerInputLine": "/Dev1/PFI0", "405 AO-TriggerInputLine": "/Dev1/PFI0", "640-Description": "DA device that is used as a shutter", "445 Shutter-TriggerSampleRate": "100000", "470 Shutter-TriggerSequenceLength": "1000", "COM1-StopBits": "1", "520 Shutter-SupportsTriggering": "0", "405-State": "0", "Core-Initialize": "1", "XLIGHT Emission Wheel-State": "7", "520 AO-Description": "NI DAC", "528 AO-TriggerInputLine": "/Dev1/PFI0", "445 Shutter-MaxVolts": "5.0000", "COM9-Handshaking": "Off", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "XLIGHT Spin motor-Label": "On (spinning)", "PRIME-PP 5 ENABLED": "No", "UUID": "b6844f77-c4e2-4e8d-bb85-fc474d3c03d6", "PRIME-TriggerMode": "Internal Trigger", "COM1-Handshaking": "Off", "PRIME-Timing-PostTriggerDelayNs": "49344000.0000", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "Focus-Position": "16479.8000", "528 Shutter-Block voltage": "false", "Channel": "Il-C", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "555 Shutter-Demo": "No", "405 Shutter-Demo": "No", "528 AO-Name": "AnalogIO", "520-State": "0", "Core-Galvo": "", "405 Shutter-TriggerSampleRate": "100000", "Focus-FocusStepSize_(nm)": "100", "PRIME-PixelType": "16bit", "COM4-DataBits": "8", "470 AO-IOChannel": "Dev1/ao2", "520 AO-MinVolts": "0.0000", "COM9-Parity": "None", "PRIME-CircularBufferEnabled": "ON", "PRIME-PP 6 Reset": "No", "640 AO-Description": "NI DAC", "Source": "PRIME", "445 AO-MinVolts": "0.0000", "PIEZO-Name": "Stage", "445 AO-Description": "NI DAC", "PRIME-CircularBufferFrameCount": "46", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "520 AO-Volts": "0.0000", "528 Shutter-TriggerSampleRate": "100000", "XPositionUm": 62321, "OpticalPath-State": "4", "PRIME-PP 3 THRESHOLD": "80", "PRIME-PVCAM Version": "3.7.1", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Gain": "1-HDR", "640 Shutter-TriggerSequenceLength": "1000", "528 AO-SupportsTriggering": "0", "445 AO-TriggerInputLine": "/Dev1/PFI0", "OpticalPath-Name": "OpticalPath", "PRIME-PP 4 ENABLED": "Yes", "PRIME-PVCAM Adapter Version": "1.3.29", "COM3-Name": "COM3", "405 AO-MaxVolts": "5.0000", "520 Shutter-Block voltage": "false", "PRIME-Timing-ClearingTimeNs": "49344000.0000", "Core-Focus": "PIEZO", "528 AO-Block voltage": "false", "TE2000-Description": "Nikon TE2000 hub", "528 AO-MinVolts": "0.0000", "COM4-Parity": "None", "Summary": { "Slices": 11, "ChContrastMin": [ 0, 0, 0, 0, 0, 0 ], "PixelAspect": 1, "SlicesFirst": true, "Interval_ms": 0, "Time": "2019-12-17 21:58:39 -0500", "Prefix": "seq_3", "InitialPositionList": [ { "Label": "Pos0", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.435], "Focus": [16480.2], "XYStage": [ 69822.40000000001, 20100.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos1", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.425], "Focus": [16480.3], "XYStage": [ 69004, 20101 ] }, "GridColumnIndex": 0 }, { "Label": "Pos2", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.44], "Focus": [16480.1], "XYStage": [ 63119.5, 20690.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos3", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.405], "Focus": [16480.2], "XYStage": [ 62321, 20690.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos4", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.415], "Focus": [16480], "XYStage": [ 56604.100000000006, 20688.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos5", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.42000000000002], "Focus": [16480.1], "XYStage": [ 56034.200000000004, 20688.800000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos6", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.455], "Focus": [16480], "XYStage": [ 50889.3, 20496.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos7", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.43], "Focus": [16480.2], "XYStage": [ 50101.600000000006, 20496.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos8", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.67000000000002], "Focus": [16479.9], "XYStage": [ 44818.600000000006, 20496.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos9", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.67000000000002], "Focus": [16480], "XYStage": [ 43897.9, 20436.800000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos10", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.675], "Focus": [16479.9], "XYStage": [ 39670.600000000006, 20664.9 ] }, "GridColumnIndex": 0 }, { "Label": "Pos11", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.69], "Focus": [16479.9], "XYStage": [ 38734, 20664.600000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos12", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.695], "Focus": [16480.1], "XYStage": [ 34037.200000000004, 20664.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos13", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.685], "Focus": [16480.1], "XYStage": [ 33152, 20664.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos14", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.69], "Focus": [16480.1], "XYStage": [ 28122.800000000003, 20664.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos15", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.675], "Focus": [16480], "XYStage": [ 27422.9, 20664.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos16", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.64000000000001], "Focus": [16480], "XYStage": [ 23005.2, 20664.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos17", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.69], "Focus": [16479.9], "XYStage": [ 21820.600000000002, 20409.600000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos18", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.41], "Focus": [16480.1], "XYStage": [ 73697.6, -13222.800000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos19", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.455], "Focus": [16480.1], "XYStage": [ 72765.8, -13223 ] }, "GridColumnIndex": 0 }, { "Label": "Pos20", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.4], "Focus": [16480.2], "XYStage": [ 67445.5, -12303.800000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos21", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.415], "Focus": [16480.2], "XYStage": [ 66613.3, -12303.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos22", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.415], "Focus": [16480.1], "XYStage": [ 61819.4, -11936.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos23", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.405], "Focus": [16480.2], "XYStage": [ 61325.700000000004, -11937.1 ] }, "GridColumnIndex": 0 }, { "Label": "Pos24", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.46], "Focus": [16480.2], "XYStage": [ 55377, -10974.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos25", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.445], "Focus": [16480.1], "XYStage": [ 54827, -11214.800000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos26", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.435], "Focus": [16480.2], "XYStage": [ 49937.100000000006, -10919.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos27", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.425], "Focus": [16480.1], "XYStage": [ 48764, -10919.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos28", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.42000000000002], "Focus": [16480.2], "XYStage": [ 43978.4, -10920.400000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos29", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.435], "Focus": [16480.2], "XYStage": [ 43106.3, -10920.400000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos30", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.405], "Focus": [16480.3], "XYStage": [ 38212.4, -10920.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos31", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.445], "Focus": [16480.2], "XYStage": [ 37254.9, -10920.400000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos32", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.44], "Focus": [16480.3], "XYStage": [ 32472.800000000003, -11294.400000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos33", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [166.95000000000002], "Focus": [16480.2], "XYStage": [ 31420.7, -11294.400000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos34", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [166.95000000000002], "Focus": [16480.2], "XYStage": [ 26881.5, -11640 ] }, "GridColumnIndex": 0 }, { "Label": "Pos35", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [166.98], "Focus": [16480.2], "XYStage": [ 25876.100000000002, -11641.1 ] }, "GridColumnIndex": 0 } ], "z-step_um": 10, "GridRow": 0, "BitDepth": 16, "Depth": 2, "Channels": 6, "Source": "Micro-Manager", "ChColors": [ -16711681, -256, -65281, -1, -1, -16776961 ], "MetadataVersion": 10, "KeepShutterOpenChannels": true, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536, 65536 ], "CustomIntervals_ms": [], "Height": 1200, "MicroManagerVersion": "1.4.23 20180219", "GridColumn": 0, "UUID": "8c8f9d20-b128-4577-9f91-89e70f22d319", "PixelType": "GRAY16", "Width": 1200, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\Yan\\20191217-YC113-S5S6", "Frames": 1, "Comment": "", "UserName": "Nikon", "PixelSize_um": 1.1, "ROI": [ 0, 0, 1200, 1200 ], "Date": "2019-12-17", "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC", "P-DAPI-high" ], "KeepShutterOpenSlices": true, "IJType": 1, "ComputerName": "ZadorConfocal2", "Positions": 36, "PositionIndex": 3, "CameraTimeout": 20000, "TimeFirst": false }, "640 AO-Name": "AnalogIO", "Vincent-D3-Command": "Close" }, "FrameKey-0-3-10": { "COM4-StopBits": "1", "528 AO-MaxVolts": "5.0000", "XLIGHT Excitation Wheel-State": "2", "470 Shutter-Name": "AnalogIO", "Core-Shutter": "640", "555 AO-Demo": "No", "XLIGHT Dichroic-State": "2", "XYStage-StepSize-Y": "0.1000", "ZPositionUm": 227.405, "XYStage-StepSize-X": "0.1000", "PRIME-FirmwareVersion": "20.28", "PRIME-ClearCycles": "2", "520-Name": "DA Shutter", "405 Shutter-Name": "AnalogIO", "PIEZO-StepSize": "0.0050", "PRIME-ClearMode": "Pre-Exposure", "470 AO-MinVolts": "0.0000", "405 AO-SupportsTriggering": "1", "CameraChannelIndex": 0, "XYStage-StartSpeed": "500.0000", "COM9-StopBits": "2", "405 AO-Description": "NI DAC", "COM4-DelayBetweenCharsMs": "0.0000", "445 AO-Block voltage": "false", "520 AO-TriggerInputLine": "/Dev1/PFI0", "555 AO-Description": "NI DAC", "520 AO-TriggerSampleRate": "100000", "COM4-Verbose": "1", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM4-Name": "COM4", "405 Shutter-Volts": "0.0000", "Vincent-D3-Port": "COM1", "PRIME-PP 4 MIN ADU AFFECTED": "200", "470 Shutter-MinVolts": "0.0000", "PRIME-PP 3 ENABLED": "Yes", "470 AO-Description": "NI DAC", "PRIME-Description": "PVCAM API device adapter", "520 Shutter-MaxVolts": "5.0000", "520 Shutter-Volts": "0.0000", "528 AO-Sequenceable": "1", "PRIME-Binning": "1x1", "555 Shutter-MaxVolts": "5.0000", "528 Shutter-TriggerSequenceLength": "1000", "405 Shutter-Block voltage": "false", "XLIGHT Spin motor-State": "1", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "Vincent-D3-Time to open (ms)": "35.0000", "Vincent-D3-Time to close (ms)": "35.0000", "445 AO-TriggerSampleRate": "100000", "640 Shutter-IOChannel": "Dev1/ao14", "555 AO-Sequenceable": "1", "520 AO-Name": "AnalogIO", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "470 AO-Block voltage": "false", "COM1-Verbose": "1", "COM9-AnswerTimeout": "10000.0000", "528 Shutter-Name": "AnalogIO", "PRIME-Trigger Timeout (secs)": "10", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "NextFrame": 0, "470 AO-TriggerSequenceLength": "1000", "PRIME-TransposeXY": "0", "445 AO-TriggerSequenceLength": "1000", "COM1-Name": "COM1", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "COM9-DataBits": "8", "COM1-AnswerTimeout": "500.0000", "Camera": "", "TE2000-Version": "V2.50-V2.50-V2.10", "528 Shutter-Volts": "0.0000", "XLIGHT Hub-Name": "XLIGHT Hub", "PIEZO-Description": "Ludl stage driver adapter", "PositionName": "Pos3", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSampleRate": "100000", "PRIME-FanSpeedSetpoint": "High", "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "405 AO-TriggerSampleRate": "100000", "470 Shutter-Block voltage": "false", "470-State": "0", "COM3-Parity": "None", "405 AO-Volts": "0.0000", "PRIME-TransposeMirrorY": "0", "PRIME-ChipName": "GS144BSI", "470 AO-Volts": "0.0000", "PRIME-TransposeMirrorX": "0", "445 Shutter-SupportsTriggering": "0", "470 Shutter-SupportsTriggering": "0", "XLIGHT Hub-Description": "XLIGHT hub", "PRIME-PreampOffLimit": "10000", "470 AO-Sequenceable": "1", "528 Shutter-MaxVolts": "5.0000", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "Core-XYStage": "XYStage", "470 AO-TriggerSampleRate": "100000", "PRIME-Timing-PreTriggerDelayNs": "24672000.0000", "528-DA Device": "528 Shutter", "Focus-Description": "Nikon TE2000 focus adapter", "FilterBlock-State": "5", "520 AO-Demo": "No", "COM3-DelayBetweenCharsMs": "0.0000", "470 Shutter-Volts": "0.0000", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "PRIME-PP 2 THRESHOLD": "125", "PRIME-PP 4 THRESHOLD": "75", "XYStage-Acceleration": "4.0000", "470 Shutter-Demo": "No", "PRIME-Name": "PMPCIECam00", "640-DA Device": "640 Shutter", "405 AO-Block voltage": "false", "ElapsedTime-ms": 158917, "PRIME-Offset": "100", "445 AO-IOChannel": "Dev1/ao1", "640 AO-MaxVolts": "5.0000", "PositionIndex": 3, "PRIME-PP 1 ENABLED": "Yes", "COM1-BaudRate": "9600", "640-State": "0", "COM3-Verbose": "1", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "LudlController-TransmissionDelay": "0", "PRIME-FullWellCapacity": "62000", "PRIME-SMARTStreamingEnabled": "No", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "640 Shutter-Description": "NI DAC", "528-Description": "DA device that is used as a shutter", "COM4-Description": "Serial port driver (boost:asio)", "405 Shutter-Description": "NI DAC", "TE2000-Name": "TE2000", "528 Shutter-Description": "NI DAC", "COM9-Description": "Serial port driver (boost:asio)", "LudlController-Name": "LudlController", "445 Shutter-TriggerSequenceLength": "1000", "PRIME-SerialNumber": "A18B203008", "445 AO-Volts": "0.0000", "PRIME-X-dimension": "1200", "PRIME-ShutterCloseDelay": "0", "XLIGHT Disk slider-State": "2", "Position": "Default", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 AO-IOChannel": "Dev1/ao5", "520 AO-IOChannel": "Dev1/ao4", "528 AO-TriggerSequenceLength": "1000", "405 Shutter-MaxVolts": "5.0000", "470-DA Device": "470 Shutter", "PRIME-FTCapable": "No", "Core-ChannelGroup": "Channels", "555 AO-SupportsTriggering": "0", "PRIME-Y-dimension": "1200", "Frame": 0, "555 Shutter-Sequenceable": "1", "PRIME-Timing-ExposureTimeNs": "9992000.0000", "Core-ImageProcessor": "", "528-State": "0", "520 AO-Block voltage": "false", "XLIGHT Excitation Wheel-Label": "QUAD", "640 Shutter-Demo": "No", "COM3-StopBits": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "520 Shutter-IOChannel": "Dev1/ao11", "Width": 1200, "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-SupportsTriggering": "0", "640 AO-TriggerSampleRate": "100000", "520-Description": "DA device that is used as a shutter", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "405 Shutter-Sequenceable": "1", "405 Shutter-SupportsTriggering": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-TriggerSampleRate": "100000", "640 Shutter-MaxVolts": "5.0000", "555 AO-MaxVolts": "5.0000", "520 AO-TriggerSequenceLength": "1000", "COM3-BaudRate": "9600", "COM9-BaudRate": "9600", "Vincent-D3-Channel #": "Ch. #1", "PRIME-PMode": "Normal", "OpticalPath-Label": "Left(100)", "BitDepth": 16, "555 AO-TriggerSequenceLength": "1000", "445 Shutter-Description": "NI DAC", "405 AO-Sequenceable": "1", "PRIME-AcquisitionMethod": "Callbacks", "555 AO-Name": "AnalogIO", "PRIME-ShutterMode": "Pre-Exposure", "COM3-Handshaking": "Off", "445 AO-SupportsTriggering": "0", "ROI": "0-0-1200-1200", "COM9-Verbose": "1", "PRIME-Color": "OFF", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "PRIME-ShutterOpenDelay": "0", "COM1-Parity": "None", "Slice": 10, "SlicePosition": 50, "COM9-DelayBetweenCharsMs": "0.0000", "555-Name": "DA Shutter", "PIEZO-LudlSingleAxisName": "B", "XLIGHT Emission Wheel-Label": "725/40 (C)", "640 Shutter-Name": "AnalogIO", "640 AO-Sequenceable": "1", "520 Shutter-Demo": "No", "528 Shutter-Demo": "No", "470-Name": "DA Shutter", "520-DA Device": "520 Shutter", "XYStage-Description": "Ludl XY stage driver adapter", "445 Shutter-MinVolts": "0.0000", "528 AO-TriggerSampleRate": "100000", "405 AO-MinVolts": "0.0000", "405 Shutter-TriggerSequenceLength": "1000", "Height": 1200, "555 AO-Block voltage": "false", "640 Shutter-Volts": "5.0000", "PixelType": "GRAY16", "445 Shutter-Block voltage": "false", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "520 Shutter-TriggerSequenceLength": "1000", "COM9-Name": "COM9", "Core-SLM": "", "555 AO-Volts": "0.0000", "555 Shutter-Block voltage": "false", "XYStage-Speed": "1800.0000", "520 AO-MaxVolts": "5.0000", "528 Shutter-SupportsTriggering": "0", "COM1-Description": "Serial port driver (boost:asio)", "PRIME-MetadataEnabled": "No", "520 Shutter-MinVolts": "0.0000", "470 Shutter-Sequenceable": "1", "PRIME-PreampDelay": "0", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-MinVolts": "0.0000", "ChannelIndex": 3, "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Core-AutoShutter": "1", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "Exposure-ms": 520, "PRIME-PP 2 ENABLED": "Yes", "640 Shutter-TriggerSampleRate": "100000", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "470 Shutter-IOChannel": "Dev1/ao10", "405 AO-Demo": "No", "PixelSizeUm": 1.1, "470 AO-Demo": "No", "470 Shutter-MaxVolts": "5.0000", "Vincent-D3-Name": "Vincent-D3", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FileName": "img_000000000_Il-C_010.tif", "LudlController-Reset": "Operate", "PRIME-Exposure": "520.00", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "445-Name": "DA Shutter", "555 Shutter-Name": "AnalogIO", "520 Shutter-TriggerSampleRate": "100000", "445 Shutter-IOChannel": "Dev1/ao9", "PRIME-TransposeCorrection": "0", "XYStage-StepSize": "0.1000", "445-Description": "DA device that is used as a shutter", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 AO-IOChannel": "Dev1/ao7", "520 Shutter-Name": "AnalogIO", "COM3-DataBits": "8", "640 AO-Volts": "4.5000", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "555 Shutter-IOChannel": "Dev1/ao13", "WaitInterval": null, "520 Shutter-Sequenceable": "1", "YPositionUm": 20690.300000000003, "445-State": "0", "445-DA Device": "445 Shutter", "PRIME-CircularBufferAutoSize": "ON", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "555 AO-TriggerSampleRate": "100000", "445 Shutter-Volts": "0.0000", "XLIGHT Hub-Port": "COM3", "Core-AutoFocus": "", "COM4-Handshaking": "Off", "528 Shutter-MinVolts": "0.0000", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "445 Shutter-Name": "AnalogIO", "528 Shutter-Sequenceable": "1", "XYStage-TransposeMirrorX": "0", "XYStage-TransposeMirrorY": "0", "PRIME-ReadoutRate": "100MHz 16bit", "XYStage-Name": "Stage", "640 AO-SupportsTriggering": "0", "640-Name": "DA Shutter", "640 Shutter-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "640 AO-Block voltage": "false", "445 Shutter-Sequenceable": "1", "470 AO-MaxVolts": "5.0000", "COM4-BaudRate": "9600", "470-Description": "DA device that is used as a shutter", "520 Shutter-Description": "NI DAC", "PRIME-CCDTemperature": "-14.9600", "Time": "2019-12-17 21:59:06 -0500", "640 AO-TriggerSequenceLength": "1000", "470 AO-Name": "AnalogIO", "XLIGHT Disk slider-Label": "Disk Pos 40um", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "Binning": "1x1", "TE2000-ModelType": "1", "COM3-AnswerTimeout": "500.0000", "555 Shutter-SupportsTriggering": "0", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "TE2000-Port": "COM4", "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Description": "NI DAC", "COM4-AnswerTimeout": "500.0000", "COM1-DelayBetweenCharsMs": "0.0000", "FilterBlock-Label": "Confocal", "445 AO-Name": "AnalogIO", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-Timing-ReadoutTimeNs": "24672000.0000", "528 AO-Demo": "No", "520 AO-Sequenceable": "1", "555-Description": "DA device that is used as a shutter", "405-DA Device": "405 Shutter", "405 AO-TriggerSequenceLength": "1000", "640 AO-MinVolts": "0.0000", "640 Shutter-Block voltage": "false", "FrameIndex": 0, "PRIME-Port": "CMOS", "555 AO-MinVolts": "0.0000", "COM3-Description": "Serial port driver (boost:asio)", "528 AO-Description": "NI DAC", "555 AO-IOChannel": "Dev1/ao6", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "555 AO-TriggerInputLine": "/Dev1/PFI0", "PRIME-ActualInterval-ms": "374.3700", "LudlController-Port": "COM9", "445 Shutter-Demo": "No", "PRIME-Trigger-Expose Out-Mux": "1", "SliceIndex": 10, "PRIME-CCDTemperatureSetPoint": "-15.0000", "405 Shutter-MinVolts": "0.0000", "PRIME-ExposeOutMode": "First Row", "AxisPositions": null, "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Volts": "0.0000", "528-Name": "DA Shutter", "555-State": "0", "XLIGHT Dichroic-Label": "Di03-R635-t1", "445 AO-Sequenceable": "1", "405-Name": "DA Shutter", "405 AO-IOChannel": "Dev1/ao0", "Core-Camera": "PRIME", "Focus-Name": "Focus", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "405 Shutter-IOChannel": "Dev1/ao8", "640 AO-Demo": "No", "640 Shutter-SupportsTriggering": "0", "445 AO-Demo": "No", "555-DA Device": "555 Shutter", "470 Shutter-Description": "NI DAC", "528 AO-Volts": "0.0000", "COM1-DataBits": "8", "Vincent-D3-Address": "x", "520 AO-SupportsTriggering": "0", "405-Description": "DA device that is used as a shutter", "640 Shutter-MinVolts": "0.0000", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "405 AO-Name": "AnalogIO", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "640 AO-TriggerInputLine": "/Dev1/PFI0", "405 AO-TriggerInputLine": "/Dev1/PFI0", "640-Description": "DA device that is used as a shutter", "445 Shutter-TriggerSampleRate": "100000", "470 Shutter-TriggerSequenceLength": "1000", "COM1-StopBits": "1", "520 Shutter-SupportsTriggering": "0", "405-State": "0", "Core-Initialize": "1", "XLIGHT Emission Wheel-State": "7", "520 AO-Description": "NI DAC", "528 AO-TriggerInputLine": "/Dev1/PFI0", "445 Shutter-MaxVolts": "5.0000", "COM9-Handshaking": "Off", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "XLIGHT Spin motor-Label": "On (spinning)", "PRIME-PP 5 ENABLED": "No", "UUID": "f2fe8043-1478-44f6-8759-50880a6a6c84", "PRIME-TriggerMode": "Internal Trigger", "COM1-Handshaking": "Off", "PRIME-Timing-PostTriggerDelayNs": "49344000.0000", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "Focus-Position": "16479.8000", "528 Shutter-Block voltage": "false", "Channel": "Il-C", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "555 Shutter-Demo": "No", "405 Shutter-Demo": "No", "528 AO-Name": "AnalogIO", "520-State": "0", "Core-Galvo": "", "405 Shutter-TriggerSampleRate": "100000", "Focus-FocusStepSize_(nm)": "100", "PRIME-PixelType": "16bit", "COM4-DataBits": "8", "470 AO-IOChannel": "Dev1/ao2", "520 AO-MinVolts": "0.0000", "COM9-Parity": "None", "PRIME-CircularBufferEnabled": "ON", "PRIME-PP 6 Reset": "No", "640 AO-Description": "NI DAC", "Source": "PRIME", "445 AO-MinVolts": "0.0000", "PIEZO-Name": "Stage", "445 AO-Description": "NI DAC", "PRIME-CircularBufferFrameCount": "46", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "520 AO-Volts": "0.0000", "528 Shutter-TriggerSampleRate": "100000", "XPositionUm": 62321, "OpticalPath-State": "4", "PRIME-PP 3 THRESHOLD": "80", "PRIME-PVCAM Version": "3.7.1", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Gain": "1-HDR", "640 Shutter-TriggerSequenceLength": "1000", "528 AO-SupportsTriggering": "0", "445 AO-TriggerInputLine": "/Dev1/PFI0", "OpticalPath-Name": "OpticalPath", "PRIME-PP 4 ENABLED": "Yes", "PRIME-PVCAM Adapter Version": "1.3.29", "COM3-Name": "COM3", "405 AO-MaxVolts": "5.0000", "520 Shutter-Block voltage": "false", "PRIME-Timing-ClearingTimeNs": "49344000.0000", "Core-Focus": "PIEZO", "528 AO-Block voltage": "false", "TE2000-Description": "Nikon TE2000 hub", "528 AO-MinVolts": "0.0000", "COM4-Parity": "None", "Summary": { "Slices": 11, "ChContrastMin": [ 0, 0, 0, 0, 0, 0 ], "PixelAspect": 1, "SlicesFirst": true, "Interval_ms": 0, "Time": "2019-12-17 21:58:39 -0500", "Prefix": "seq_3", "InitialPositionList": [ { "Label": "Pos0", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.435], "Focus": [16480.2], "XYStage": [ 69822.40000000001, 20100.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos1", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.425], "Focus": [16480.3], "XYStage": [ 69004, 20101 ] }, "GridColumnIndex": 0 }, { "Label": "Pos2", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.44], "Focus": [16480.1], "XYStage": [ 63119.5, 20690.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos3", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.405], "Focus": [16480.2], "XYStage": [ 62321, 20690.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos4", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.415], "Focus": [16480], "XYStage": [ 56604.100000000006, 20688.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos5", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.42000000000002], "Focus": [16480.1], "XYStage": [ 56034.200000000004, 20688.800000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos6", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.455], "Focus": [16480], "XYStage": [ 50889.3, 20496.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos7", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.43], "Focus": [16480.2], "XYStage": [ 50101.600000000006, 20496.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos8", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.67000000000002], "Focus": [16479.9], "XYStage": [ 44818.600000000006, 20496.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos9", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.67000000000002], "Focus": [16480], "XYStage": [ 43897.9, 20436.800000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos10", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.675], "Focus": [16479.9], "XYStage": [ 39670.600000000006, 20664.9 ] }, "GridColumnIndex": 0 }, { "Label": "Pos11", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.69], "Focus": [16479.9], "XYStage": [ 38734, 20664.600000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos12", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.695], "Focus": [16480.1], "XYStage": [ 34037.200000000004, 20664.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos13", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.685], "Focus": [16480.1], "XYStage": [ 33152, 20664.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos14", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.69], "Focus": [16480.1], "XYStage": [ 28122.800000000003, 20664.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos15", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.675], "Focus": [16480], "XYStage": [ 27422.9, 20664.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos16", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.64000000000001], "Focus": [16480], "XYStage": [ 23005.2, 20664.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos17", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.69], "Focus": [16479.9], "XYStage": [ 21820.600000000002, 20409.600000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos18", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.41], "Focus": [16480.1], "XYStage": [ 73697.6, -13222.800000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos19", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.455], "Focus": [16480.1], "XYStage": [ 72765.8, -13223 ] }, "GridColumnIndex": 0 }, { "Label": "Pos20", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.4], "Focus": [16480.2], "XYStage": [ 67445.5, -12303.800000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos21", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.415], "Focus": [16480.2], "XYStage": [ 66613.3, -12303.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos22", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.415], "Focus": [16480.1], "XYStage": [ 61819.4, -11936.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos23", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.405], "Focus": [16480.2], "XYStage": [ 61325.700000000004, -11937.1 ] }, "GridColumnIndex": 0 }, { "Label": "Pos24", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.46], "Focus": [16480.2], "XYStage": [ 55377, -10974.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos25", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.445], "Focus": [16480.1], "XYStage": [ 54827, -11214.800000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos26", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.435], "Focus": [16480.2], "XYStage": [ 49937.100000000006, -10919.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos27", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.425], "Focus": [16480.1], "XYStage": [ 48764, -10919.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos28", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.42000000000002], "Focus": [16480.2], "XYStage": [ 43978.4, -10920.400000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos29", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.435], "Focus": [16480.2], "XYStage": [ 43106.3, -10920.400000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos30", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.405], "Focus": [16480.3], "XYStage": [ 38212.4, -10920.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos31", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.445], "Focus": [16480.2], "XYStage": [ 37254.9, -10920.400000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos32", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.44], "Focus": [16480.3], "XYStage": [ 32472.800000000003, -11294.400000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos33", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [166.95000000000002], "Focus": [16480.2], "XYStage": [ 31420.7, -11294.400000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos34", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [166.95000000000002], "Focus": [16480.2], "XYStage": [ 26881.5, -11640 ] }, "GridColumnIndex": 0 }, { "Label": "Pos35", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [166.98], "Focus": [16480.2], "XYStage": [ 25876.100000000002, -11641.1 ] }, "GridColumnIndex": 0 } ], "z-step_um": 10, "GridRow": 0, "BitDepth": 16, "Depth": 2, "Channels": 6, "Source": "Micro-Manager", "ChColors": [ -16711681, -256, -65281, -1, -1, -16776961 ], "MetadataVersion": 10, "KeepShutterOpenChannels": true, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536, 65536 ], "CustomIntervals_ms": [], "Height": 1200, "MicroManagerVersion": "1.4.23 20180219", "GridColumn": 0, "UUID": "8c8f9d20-b128-4577-9f91-89e70f22d319", "PixelType": "GRAY16", "Width": 1200, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\Yan\\20191217-YC113-S5S6", "Frames": 1, "Comment": "", "UserName": "Nikon", "PixelSize_um": 1.1, "ROI": [ 0, 0, 1200, 1200 ], "Date": "2019-12-17", "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC", "P-DAPI-high" ], "KeepShutterOpenSlices": true, "IJType": 1, "ComputerName": "ZadorConfocal2", "Positions": 36, "PositionIndex": 3, "CameraTimeout": 20000, "TimeFirst": false }, "640 AO-Name": "AnalogIO", "Vincent-D3-Command": "Close" }, "FrameKey-0-4-0": { "COM4-StopBits": "1", "528 AO-MaxVolts": "5.0000", "XLIGHT Excitation Wheel-State": "2", "470 Shutter-Name": "AnalogIO", "Core-Shutter": "Vincent-D3", "555 AO-Demo": "No", "XLIGHT Dichroic-State": "2", "XYStage-StepSize-Y": "0.1000", "ZPositionUm": 127.405, "XYStage-StepSize-X": "0.1000", "PRIME-FirmwareVersion": "20.28", "PRIME-ClearCycles": "2", "520-Name": "DA Shutter", "405 Shutter-Name": "AnalogIO", "PIEZO-StepSize": "0.0050", "PRIME-ClearMode": "Pre-Exposure", "470 AO-MinVolts": "0.0000", "405 AO-SupportsTriggering": "1", "CameraChannelIndex": 0, "XYStage-StartSpeed": "500.0000", "COM9-StopBits": "2", "405 AO-Description": "NI DAC", "COM4-DelayBetweenCharsMs": "0.0000", "445 AO-Block voltage": "false", "520 AO-TriggerInputLine": "/Dev1/PFI0", "555 AO-Description": "NI DAC", "520 AO-TriggerSampleRate": "100000", "COM4-Verbose": "1", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM4-Name": "COM4", "405 Shutter-Volts": "0.0000", "Vincent-D3-Port": "COM1", "PRIME-PP 4 MIN ADU AFFECTED": "200", "470 Shutter-MinVolts": "0.0000", "PRIME-PP 3 ENABLED": "Yes", "470 AO-Description": "NI DAC", "PRIME-Description": "PVCAM API device adapter", "520 Shutter-MaxVolts": "5.0000", "520 Shutter-Volts": "0.0000", "528 AO-Sequenceable": "1", "PRIME-Binning": "1x1", "555 Shutter-MaxVolts": "5.0000", "528 Shutter-TriggerSequenceLength": "1000", "405 Shutter-Block voltage": "false", "XLIGHT Spin motor-State": "1", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "Vincent-D3-Time to open (ms)": "35.0000", "Vincent-D3-Time to close (ms)": "35.0000", "445 AO-TriggerSampleRate": "100000", "640 Shutter-IOChannel": "Dev1/ao14", "555 AO-Sequenceable": "1", "520 AO-Name": "AnalogIO", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "470 AO-Block voltage": "false", "COM1-Verbose": "1", "COM9-AnswerTimeout": "10000.0000", "528 Shutter-Name": "AnalogIO", "PRIME-Trigger Timeout (secs)": "10", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "NextFrame": 0, "470 AO-TriggerSequenceLength": "1000", "PRIME-TransposeXY": "0", "445 AO-TriggerSequenceLength": "1000", "COM1-Name": "COM1", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "COM9-DataBits": "8", "COM1-AnswerTimeout": "500.0000", "Camera": "", "TE2000-Version": "V2.50-V2.50-V2.10", "528 Shutter-Volts": "0.0000", "XLIGHT Hub-Name": "XLIGHT Hub", "PIEZO-Description": "Ludl stage driver adapter", "PositionName": "Pos3", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSampleRate": "100000", "PRIME-FanSpeedSetpoint": "High", "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "405 AO-TriggerSampleRate": "100000", "470 Shutter-Block voltage": "false", "470-State": "0", "COM3-Parity": "None", "405 AO-Volts": "0.0000", "PRIME-TransposeMirrorY": "0", "PRIME-ChipName": "GS144BSI", "470 AO-Volts": "0.0000", "PRIME-TransposeMirrorX": "0", "445 Shutter-SupportsTriggering": "0", "470 Shutter-SupportsTriggering": "0", "XLIGHT Hub-Description": "XLIGHT hub", "PRIME-PreampOffLimit": "10000", "470 AO-Sequenceable": "1", "528 Shutter-MaxVolts": "5.0000", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "Core-XYStage": "XYStage", "470 AO-TriggerSampleRate": "100000", "PRIME-Timing-PreTriggerDelayNs": "24672000.0000", "528-DA Device": "528 Shutter", "Focus-Description": "Nikon TE2000 focus adapter", "FilterBlock-State": "5", "520 AO-Demo": "No", "COM3-DelayBetweenCharsMs": "0.0000", "470 Shutter-Volts": "0.0000", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "PRIME-PP 2 THRESHOLD": "125", "PRIME-PP 4 THRESHOLD": "75", "XYStage-Acceleration": "4.0000", "470 Shutter-Demo": "No", "PRIME-Name": "PMPCIECam00", "640-DA Device": "640 Shutter", "405 AO-Block voltage": "false", "ElapsedTime-ms": 159930, "PRIME-Offset": "100", "445 AO-IOChannel": "Dev1/ao1", "640 AO-MaxVolts": "5.0000", "PositionIndex": 3, "PRIME-PP 1 ENABLED": "Yes", "COM1-BaudRate": "9600", "640-State": "0", "COM3-Verbose": "1", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "LudlController-TransmissionDelay": "0", "PRIME-FullWellCapacity": "62000", "PRIME-SMARTStreamingEnabled": "No", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "640 Shutter-Description": "NI DAC", "528-Description": "DA device that is used as a shutter", "COM4-Description": "Serial port driver (boost:asio)", "405 Shutter-Description": "NI DAC", "TE2000-Name": "TE2000", "528 Shutter-Description": "NI DAC", "COM9-Description": "Serial port driver (boost:asio)", "LudlController-Name": "LudlController", "445 Shutter-TriggerSequenceLength": "1000", "PRIME-SerialNumber": "A18B203008", "445 AO-Volts": "0.0000", "PRIME-X-dimension": "1200", "PRIME-ShutterCloseDelay": "0", "XLIGHT Disk slider-State": "2", "Position": "Default", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 AO-IOChannel": "Dev1/ao5", "520 AO-IOChannel": "Dev1/ao4", "528 AO-TriggerSequenceLength": "1000", "405 Shutter-MaxVolts": "5.0000", "470-DA Device": "470 Shutter", "PRIME-FTCapable": "No", "Core-ChannelGroup": "Channels", "555 AO-SupportsTriggering": "0", "PRIME-Y-dimension": "1200", "Frame": 0, "555 Shutter-Sequenceable": "1", "PRIME-Timing-ExposureTimeNs": "9992000.0000", "Core-ImageProcessor": "", "528-State": "0", "520 AO-Block voltage": "false", "XLIGHT Excitation Wheel-Label": "Excitation-2", "640 Shutter-Demo": "No", "COM3-StopBits": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "520 Shutter-IOChannel": "Dev1/ao11", "Width": 1200, "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-SupportsTriggering": "0", "640 AO-TriggerSampleRate": "100000", "520-Description": "DA device that is used as a shutter", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "405 Shutter-Sequenceable": "1", "405 Shutter-SupportsTriggering": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-TriggerSampleRate": "100000", "640 Shutter-MaxVolts": "5.0000", "555 AO-MaxVolts": "5.0000", "520 AO-TriggerSequenceLength": "1000", "COM3-BaudRate": "9600", "COM9-BaudRate": "9600", "Vincent-D3-Channel #": "Ch. #1", "PRIME-PMode": "Normal", "OpticalPath-Label": "Left(100)", "BitDepth": 16, "555 AO-TriggerSequenceLength": "1000", "445 Shutter-Description": "NI DAC", "405 AO-Sequenceable": "1", "PRIME-AcquisitionMethod": "Callbacks", "555 AO-Name": "AnalogIO", "PRIME-ShutterMode": "Pre-Exposure", "COM3-Handshaking": "Off", "445 AO-SupportsTriggering": "0", "ROI": "0-0-1200-1200", "COM9-Verbose": "1", "PRIME-Color": "OFF", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "PRIME-ShutterOpenDelay": "0", "COM1-Parity": "None", "Slice": 0, "SlicePosition": -50, "COM9-DelayBetweenCharsMs": "0.0000", "555-Name": "DA Shutter", "PIEZO-LudlSingleAxisName": "B", "XLIGHT Emission Wheel-Label": "Empty", "640 Shutter-Name": "AnalogIO", "640 AO-Sequenceable": "1", "520 Shutter-Demo": "No", "528 Shutter-Demo": "No", "470-Name": "DA Shutter", "520-DA Device": "520 Shutter", "XYStage-Description": "Ludl XY stage driver adapter", "445 Shutter-MinVolts": "0.0000", "528 AO-TriggerSampleRate": "100000", "405 AO-MinVolts": "0.0000", "405 Shutter-TriggerSequenceLength": "1000", "Height": 1200, "555 AO-Block voltage": "false", "640 Shutter-Volts": "0.0000", "PixelType": "GRAY16", "445 Shutter-Block voltage": "false", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "520 Shutter-TriggerSequenceLength": "1000", "COM9-Name": "COM9", "Core-SLM": "", "555 AO-Volts": "0.0000", "555 Shutter-Block voltage": "false", "XYStage-Speed": "1800.0000", "520 AO-MaxVolts": "5.0000", "528 Shutter-SupportsTriggering": "0", "COM1-Description": "Serial port driver (boost:asio)", "PRIME-MetadataEnabled": "No", "520 Shutter-MinVolts": "0.0000", "470 Shutter-Sequenceable": "1", "PRIME-PreampDelay": "0", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-MinVolts": "0.0000", "ChannelIndex": 4, "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Core-AutoShutter": "1", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "Exposure-ms": 20, "PRIME-PP 2 ENABLED": "Yes", "640 Shutter-TriggerSampleRate": "100000", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "470 Shutter-IOChannel": "Dev1/ao10", "405 AO-Demo": "No", "PixelSizeUm": 1.1, "470 AO-Demo": "No", "470 Shutter-MaxVolts": "5.0000", "Vincent-D3-Name": "Vincent-D3", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FileName": "img_000000000_P-DIC_000.tif", "LudlController-Reset": "Operate", "PRIME-Exposure": "20.00", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "445-Name": "DA Shutter", "555 Shutter-Name": "AnalogIO", "520 Shutter-TriggerSampleRate": "100000", "445 Shutter-IOChannel": "Dev1/ao9", "PRIME-TransposeCorrection": "0", "XYStage-StepSize": "0.1000", "445-Description": "DA device that is used as a shutter", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 AO-IOChannel": "Dev1/ao7", "520 Shutter-Name": "AnalogIO", "COM3-DataBits": "8", "640 AO-Volts": "0.0000", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "555 Shutter-IOChannel": "Dev1/ao13", "WaitInterval": null, "520 Shutter-Sequenceable": "1", "YPositionUm": 20690.300000000003, "445-State": "0", "445-DA Device": "445 Shutter", "PRIME-CircularBufferAutoSize": "ON", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "555 AO-TriggerSampleRate": "100000", "445 Shutter-Volts": "0.0000", "XLIGHT Hub-Port": "COM3", "Core-AutoFocus": "", "COM4-Handshaking": "Off", "528 Shutter-MinVolts": "0.0000", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "445 Shutter-Name": "AnalogIO", "528 Shutter-Sequenceable": "1", "XYStage-TransposeMirrorX": "0", "XYStage-TransposeMirrorY": "0", "PRIME-ReadoutRate": "100MHz 16bit", "XYStage-Name": "Stage", "640 AO-SupportsTriggering": "0", "640-Name": "DA Shutter", "640 Shutter-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "640 AO-Block voltage": "false", "445 Shutter-Sequenceable": "1", "470 AO-MaxVolts": "5.0000", "COM4-BaudRate": "9600", "470-Description": "DA device that is used as a shutter", "520 Shutter-Description": "NI DAC", "PRIME-CCDTemperature": "-14.9600", "Time": "2019-12-17 21:59:07 -0500", "640 AO-TriggerSequenceLength": "1000", "470 AO-Name": "AnalogIO", "XLIGHT Disk slider-Label": "Disk Pos 40um", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "Binning": "1x1", "TE2000-ModelType": "1", "COM3-AnswerTimeout": "500.0000", "555 Shutter-SupportsTriggering": "0", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "TE2000-Port": "COM4", "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Description": "NI DAC", "COM4-AnswerTimeout": "500.0000", "COM1-DelayBetweenCharsMs": "0.0000", "FilterBlock-Label": "Confocal", "445 AO-Name": "AnalogIO", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-Timing-ReadoutTimeNs": "24672000.0000", "528 AO-Demo": "No", "520 AO-Sequenceable": "1", "555-Description": "DA device that is used as a shutter", "405-DA Device": "405 Shutter", "405 AO-TriggerSequenceLength": "1000", "640 AO-MinVolts": "0.0000", "640 Shutter-Block voltage": "false", "FrameIndex": 0, "PRIME-Port": "CMOS", "555 AO-MinVolts": "0.0000", "COM3-Description": "Serial port driver (boost:asio)", "528 AO-Description": "NI DAC", "555 AO-IOChannel": "Dev1/ao6", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "555 AO-TriggerInputLine": "/Dev1/PFI0", "PRIME-ActualInterval-ms": "374.3700", "LudlController-Port": "COM9", "445 Shutter-Demo": "No", "PRIME-Trigger-Expose Out-Mux": "1", "SliceIndex": 0, "PRIME-CCDTemperatureSetPoint": "-15.0000", "405 Shutter-MinVolts": "0.0000", "PRIME-ExposeOutMode": "First Row", "AxisPositions": null, "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Volts": "0.0000", "528-Name": "DA Shutter", "555-State": "0", "XLIGHT Dichroic-Label": "Di03-R635-t1", "445 AO-Sequenceable": "1", "405-Name": "DA Shutter", "405 AO-IOChannel": "Dev1/ao0", "Core-Camera": "PRIME", "Focus-Name": "Focus", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "405 Shutter-IOChannel": "Dev1/ao8", "640 AO-Demo": "No", "640 Shutter-SupportsTriggering": "0", "445 AO-Demo": "No", "555-DA Device": "555 Shutter", "470 Shutter-Description": "NI DAC", "528 AO-Volts": "0.0000", "COM1-DataBits": "8", "Vincent-D3-Address": "x", "520 AO-SupportsTriggering": "0", "405-Description": "DA device that is used as a shutter", "640 Shutter-MinVolts": "0.0000", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "405 AO-Name": "AnalogIO", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "640 AO-TriggerInputLine": "/Dev1/PFI0", "405 AO-TriggerInputLine": "/Dev1/PFI0", "640-Description": "DA device that is used as a shutter", "445 Shutter-TriggerSampleRate": "100000", "470 Shutter-TriggerSequenceLength": "1000", "COM1-StopBits": "1", "520 Shutter-SupportsTriggering": "0", "405-State": "0", "Core-Initialize": "1", "XLIGHT Emission Wheel-State": "7", "520 AO-Description": "NI DAC", "528 AO-TriggerInputLine": "/Dev1/PFI0", "445 Shutter-MaxVolts": "5.0000", "COM9-Handshaking": "Off", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "XLIGHT Spin motor-Label": "On (spinning)", "PRIME-PP 5 ENABLED": "No", "UUID": "ae85c7dc-91f0-450a-a483-f617b776c7cb", "PRIME-TriggerMode": "Internal Trigger", "COM1-Handshaking": "Off", "PRIME-Timing-PostTriggerDelayNs": "49344000.0000", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "Focus-Position": "16479.8000", "528 Shutter-Block voltage": "false", "Channel": "P-DIC", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "555 Shutter-Demo": "No", "405 Shutter-Demo": "No", "528 AO-Name": "AnalogIO", "520-State": "0", "Core-Galvo": "", "405 Shutter-TriggerSampleRate": "100000", "Focus-FocusStepSize_(nm)": "100", "PRIME-PixelType": "16bit", "COM4-DataBits": "8", "470 AO-IOChannel": "Dev1/ao2", "520 AO-MinVolts": "0.0000", "COM9-Parity": "None", "PRIME-CircularBufferEnabled": "ON", "PRIME-PP 6 Reset": "No", "640 AO-Description": "NI DAC", "Source": "PRIME", "445 AO-MinVolts": "0.0000", "PIEZO-Name": "Stage", "445 AO-Description": "NI DAC", "PRIME-CircularBufferFrameCount": "46", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "520 AO-Volts": "0.0000", "528 Shutter-TriggerSampleRate": "100000", "XPositionUm": 62321, "OpticalPath-State": "4", "PRIME-PP 3 THRESHOLD": "80", "PRIME-PVCAM Version": "3.7.1", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Gain": "1-HDR", "640 Shutter-TriggerSequenceLength": "1000", "528 AO-SupportsTriggering": "0", "445 AO-TriggerInputLine": "/Dev1/PFI0", "OpticalPath-Name": "OpticalPath", "PRIME-PP 4 ENABLED": "Yes", "PRIME-PVCAM Adapter Version": "1.3.29", "COM3-Name": "COM3", "405 AO-MaxVolts": "5.0000", "520 Shutter-Block voltage": "false", "PRIME-Timing-ClearingTimeNs": "49344000.0000", "Core-Focus": "PIEZO", "528 AO-Block voltage": "false", "TE2000-Description": "Nikon TE2000 hub", "528 AO-MinVolts": "0.0000", "COM4-Parity": "None", "Summary": { "Slices": 11, "ChContrastMin": [ 0, 0, 0, 0, 0, 0 ], "PixelAspect": 1, "SlicesFirst": true, "Interval_ms": 0, "Time": "2019-12-17 21:58:39 -0500", "Prefix": "seq_3", "InitialPositionList": [ { "Label": "Pos0", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.435], "Focus": [16480.2], "XYStage": [ 69822.40000000001, 20100.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos1", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.425], "Focus": [16480.3], "XYStage": [ 69004, 20101 ] }, "GridColumnIndex": 0 }, { "Label": "Pos2", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.44], "Focus": [16480.1], "XYStage": [ 63119.5, 20690.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos3", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.405], "Focus": [16480.2], "XYStage": [ 62321, 20690.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos4", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.415], "Focus": [16480], "XYStage": [ 56604.100000000006, 20688.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos5", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.42000000000002], "Focus": [16480.1], "XYStage": [ 56034.200000000004, 20688.800000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos6", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.455], "Focus": [16480], "XYStage": [ 50889.3, 20496.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos7", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.43], "Focus": [16480.2], "XYStage": [ 50101.600000000006, 20496.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos8", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.67000000000002], "Focus": [16479.9], "XYStage": [ 44818.600000000006, 20496.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos9", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.67000000000002], "Focus": [16480], "XYStage": [ 43897.9, 20436.800000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos10", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.675], "Focus": [16479.9], "XYStage": [ 39670.600000000006, 20664.9 ] }, "GridColumnIndex": 0 }, { "Label": "Pos11", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.69], "Focus": [16479.9], "XYStage": [ 38734, 20664.600000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos12", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.695], "Focus": [16480.1], "XYStage": [ 34037.200000000004, 20664.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos13", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.685], "Focus": [16480.1], "XYStage": [ 33152, 20664.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos14", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.69], "Focus": [16480.1], "XYStage": [ 28122.800000000003, 20664.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos15", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.675], "Focus": [16480], "XYStage": [ 27422.9, 20664.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos16", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.64000000000001], "Focus": [16480], "XYStage": [ 23005.2, 20664.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos17", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.69], "Focus": [16479.9], "XYStage": [ 21820.600000000002, 20409.600000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos18", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.41], "Focus": [16480.1], "XYStage": [ 73697.6, -13222.800000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos19", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.455], "Focus": [16480.1], "XYStage": [ 72765.8, -13223 ] }, "GridColumnIndex": 0 }, { "Label": "Pos20", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.4], "Focus": [16480.2], "XYStage": [ 67445.5, -12303.800000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos21", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.415], "Focus": [16480.2], "XYStage": [ 66613.3, -12303.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos22", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.415], "Focus": [16480.1], "XYStage": [ 61819.4, -11936.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos23", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.405], "Focus": [16480.2], "XYStage": [ 61325.700000000004, -11937.1 ] }, "GridColumnIndex": 0 }, { "Label": "Pos24", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.46], "Focus": [16480.2], "XYStage": [ 55377, -10974.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos25", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.445], "Focus": [16480.1], "XYStage": [ 54827, -11214.800000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos26", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.435], "Focus": [16480.2], "XYStage": [ 49937.100000000006, -10919.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos27", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.425], "Focus": [16480.1], "XYStage": [ 48764, -10919.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos28", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.42000000000002], "Focus": [16480.2], "XYStage": [ 43978.4, -10920.400000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos29", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.435], "Focus": [16480.2], "XYStage": [ 43106.3, -10920.400000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos30", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.405], "Focus": [16480.3], "XYStage": [ 38212.4, -10920.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos31", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.445], "Focus": [16480.2], "XYStage": [ 37254.9, -10920.400000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos32", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.44], "Focus": [16480.3], "XYStage": [ 32472.800000000003, -11294.400000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos33", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [166.95000000000002], "Focus": [16480.2], "XYStage": [ 31420.7, -11294.400000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos34", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [166.95000000000002], "Focus": [16480.2], "XYStage": [ 26881.5, -11640 ] }, "GridColumnIndex": 0 }, { "Label": "Pos35", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [166.98], "Focus": [16480.2], "XYStage": [ 25876.100000000002, -11641.1 ] }, "GridColumnIndex": 0 } ], "z-step_um": 10, "GridRow": 0, "BitDepth": 16, "Depth": 2, "Channels": 6, "Source": "Micro-Manager", "ChColors": [ -16711681, -256, -65281, -1, -1, -16776961 ], "MetadataVersion": 10, "KeepShutterOpenChannels": true, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536, 65536 ], "CustomIntervals_ms": [], "Height": 1200, "MicroManagerVersion": "1.4.23 20180219", "GridColumn": 0, "UUID": "8c8f9d20-b128-4577-9f91-89e70f22d319", "PixelType": "GRAY16", "Width": 1200, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\Yan\\20191217-YC113-S5S6", "Frames": 1, "Comment": "", "UserName": "Nikon", "PixelSize_um": 1.1, "ROI": [ 0, 0, 1200, 1200 ], "Date": "2019-12-17", "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC", "P-DAPI-high" ], "KeepShutterOpenSlices": true, "IJType": 1, "ComputerName": "ZadorConfocal2", "Positions": 36, "PositionIndex": 3, "CameraTimeout": 20000, "TimeFirst": false }, "640 AO-Name": "AnalogIO", "Vincent-D3-Command": "Close" }, "FrameKey-0-4-1": { "COM4-StopBits": "1", "528 AO-MaxVolts": "5.0000", "XLIGHT Excitation Wheel-State": "2", "470 Shutter-Name": "AnalogIO", "Core-Shutter": "Vincent-D3", "555 AO-Demo": "No", "XLIGHT Dichroic-State": "2", "XYStage-StepSize-Y": "0.1000", "ZPositionUm": 137.405, "XYStage-StepSize-X": "0.1000", "PRIME-FirmwareVersion": "20.28", "PRIME-ClearCycles": "2", "520-Name": "DA Shutter", "405 Shutter-Name": "AnalogIO", "PIEZO-StepSize": "0.0050", "PRIME-ClearMode": "Pre-Exposure", "470 AO-MinVolts": "0.0000", "405 AO-SupportsTriggering": "1", "CameraChannelIndex": 0, "XYStage-StartSpeed": "500.0000", "COM9-StopBits": "2", "405 AO-Description": "NI DAC", "COM4-DelayBetweenCharsMs": "0.0000", "445 AO-Block voltage": "false", "520 AO-TriggerInputLine": "/Dev1/PFI0", "555 AO-Description": "NI DAC", "520 AO-TriggerSampleRate": "100000", "COM4-Verbose": "1", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM4-Name": "COM4", "405 Shutter-Volts": "0.0000", "Vincent-D3-Port": "COM1", "PRIME-PP 4 MIN ADU AFFECTED": "200", "470 Shutter-MinVolts": "0.0000", "PRIME-PP 3 ENABLED": "Yes", "470 AO-Description": "NI DAC", "PRIME-Description": "PVCAM API device adapter", "520 Shutter-MaxVolts": "5.0000", "520 Shutter-Volts": "0.0000", "528 AO-Sequenceable": "1", "PRIME-Binning": "1x1", "555 Shutter-MaxVolts": "5.0000", "528 Shutter-TriggerSequenceLength": "1000", "405 Shutter-Block voltage": "false", "XLIGHT Spin motor-State": "1", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "Vincent-D3-Time to open (ms)": "35.0000", "Vincent-D3-Time to close (ms)": "35.0000", "445 AO-TriggerSampleRate": "100000", "640 Shutter-IOChannel": "Dev1/ao14", "555 AO-Sequenceable": "1", "520 AO-Name": "AnalogIO", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "470 AO-Block voltage": "false", "COM1-Verbose": "1", "COM9-AnswerTimeout": "10000.0000", "528 Shutter-Name": "AnalogIO", "PRIME-Trigger Timeout (secs)": "10", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "NextFrame": 0, "470 AO-TriggerSequenceLength": "1000", "PRIME-TransposeXY": "0", "445 AO-TriggerSequenceLength": "1000", "COM1-Name": "COM1", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "COM9-DataBits": "8", "COM1-AnswerTimeout": "500.0000", "Camera": "", "TE2000-Version": "V2.50-V2.50-V2.10", "528 Shutter-Volts": "0.0000", "XLIGHT Hub-Name": "XLIGHT Hub", "PIEZO-Description": "Ludl stage driver adapter", "PositionName": "Pos3", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSampleRate": "100000", "PRIME-FanSpeedSetpoint": "High", "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "405 AO-TriggerSampleRate": "100000", "470 Shutter-Block voltage": "false", "470-State": "0", "COM3-Parity": "None", "405 AO-Volts": "0.0000", "PRIME-TransposeMirrorY": "0", "PRIME-ChipName": "GS144BSI", "470 AO-Volts": "0.0000", "PRIME-TransposeMirrorX": "0", "445 Shutter-SupportsTriggering": "0", "470 Shutter-SupportsTriggering": "0", "XLIGHT Hub-Description": "XLIGHT hub", "PRIME-PreampOffLimit": "10000", "470 AO-Sequenceable": "1", "528 Shutter-MaxVolts": "5.0000", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "Core-XYStage": "XYStage", "470 AO-TriggerSampleRate": "100000", "PRIME-Timing-PreTriggerDelayNs": "24672000.0000", "528-DA Device": "528 Shutter", "Focus-Description": "Nikon TE2000 focus adapter", "FilterBlock-State": "5", "520 AO-Demo": "No", "COM3-DelayBetweenCharsMs": "0.0000", "470 Shutter-Volts": "0.0000", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "PRIME-PP 2 THRESHOLD": "125", "PRIME-PP 4 THRESHOLD": "75", "XYStage-Acceleration": "4.0000", "470 Shutter-Demo": "No", "PRIME-Name": "PMPCIECam00", "640-DA Device": "640 Shutter", "405 AO-Block voltage": "false", "ElapsedTime-ms": 160188, "PRIME-Offset": "100", "445 AO-IOChannel": "Dev1/ao1", "640 AO-MaxVolts": "5.0000", "PositionIndex": 3, "PRIME-PP 1 ENABLED": "Yes", "COM1-BaudRate": "9600", "640-State": "0", "COM3-Verbose": "1", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "LudlController-TransmissionDelay": "0", "PRIME-FullWellCapacity": "62000", "PRIME-SMARTStreamingEnabled": "No", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "640 Shutter-Description": "NI DAC", "528-Description": "DA device that is used as a shutter", "COM4-Description": "Serial port driver (boost:asio)", "405 Shutter-Description": "NI DAC", "TE2000-Name": "TE2000", "528 Shutter-Description": "NI DAC", "COM9-Description": "Serial port driver (boost:asio)", "LudlController-Name": "LudlController", "445 Shutter-TriggerSequenceLength": "1000", "PRIME-SerialNumber": "A18B203008", "445 AO-Volts": "0.0000", "PRIME-X-dimension": "1200", "PRIME-ShutterCloseDelay": "0", "XLIGHT Disk slider-State": "2", "Position": "Default", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 AO-IOChannel": "Dev1/ao5", "520 AO-IOChannel": "Dev1/ao4", "528 AO-TriggerSequenceLength": "1000", "405 Shutter-MaxVolts": "5.0000", "470-DA Device": "470 Shutter", "PRIME-FTCapable": "No", "Core-ChannelGroup": "Channels", "555 AO-SupportsTriggering": "0", "PRIME-Y-dimension": "1200", "Frame": 0, "555 Shutter-Sequenceable": "1", "PRIME-Timing-ExposureTimeNs": "9992000.0000", "Core-ImageProcessor": "", "528-State": "0", "520 AO-Block voltage": "false", "XLIGHT Excitation Wheel-Label": "Excitation-2", "640 Shutter-Demo": "No", "COM3-StopBits": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "520 Shutter-IOChannel": "Dev1/ao11", "Width": 1200, "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-SupportsTriggering": "0", "640 AO-TriggerSampleRate": "100000", "520-Description": "DA device that is used as a shutter", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "405 Shutter-Sequenceable": "1", "405 Shutter-SupportsTriggering": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-TriggerSampleRate": "100000", "640 Shutter-MaxVolts": "5.0000", "555 AO-MaxVolts": "5.0000", "520 AO-TriggerSequenceLength": "1000", "COM3-BaudRate": "9600", "COM9-BaudRate": "9600", "Vincent-D3-Channel #": "Ch. #1", "PRIME-PMode": "Normal", "OpticalPath-Label": "Left(100)", "BitDepth": 16, "555 AO-TriggerSequenceLength": "1000", "445 Shutter-Description": "NI DAC", "405 AO-Sequenceable": "1", "PRIME-AcquisitionMethod": "Callbacks", "555 AO-Name": "AnalogIO", "PRIME-ShutterMode": "Pre-Exposure", "COM3-Handshaking": "Off", "445 AO-SupportsTriggering": "0", "ROI": "0-0-1200-1200", "COM9-Verbose": "1", "PRIME-Color": "OFF", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "PRIME-ShutterOpenDelay": "0", "COM1-Parity": "None", "Slice": 1, "SlicePosition": -40, "COM9-DelayBetweenCharsMs": "0.0000", "555-Name": "DA Shutter", "PIEZO-LudlSingleAxisName": "B", "XLIGHT Emission Wheel-Label": "Empty", "640 Shutter-Name": "AnalogIO", "640 AO-Sequenceable": "1", "520 Shutter-Demo": "No", "528 Shutter-Demo": "No", "470-Name": "DA Shutter", "520-DA Device": "520 Shutter", "XYStage-Description": "Ludl XY stage driver adapter", "445 Shutter-MinVolts": "0.0000", "528 AO-TriggerSampleRate": "100000", "405 AO-MinVolts": "0.0000", "405 Shutter-TriggerSequenceLength": "1000", "Height": 1200, "555 AO-Block voltage": "false", "640 Shutter-Volts": "0.0000", "PixelType": "GRAY16", "445 Shutter-Block voltage": "false", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "520 Shutter-TriggerSequenceLength": "1000", "COM9-Name": "COM9", "Core-SLM": "", "555 AO-Volts": "0.0000", "555 Shutter-Block voltage": "false", "XYStage-Speed": "1800.0000", "520 AO-MaxVolts": "5.0000", "528 Shutter-SupportsTriggering": "0", "COM1-Description": "Serial port driver (boost:asio)", "PRIME-MetadataEnabled": "No", "520 Shutter-MinVolts": "0.0000", "470 Shutter-Sequenceable": "1", "PRIME-PreampDelay": "0", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-MinVolts": "0.0000", "ChannelIndex": 4, "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Core-AutoShutter": "1", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "Exposure-ms": 20, "PRIME-PP 2 ENABLED": "Yes", "640 Shutter-TriggerSampleRate": "100000", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "470 Shutter-IOChannel": "Dev1/ao10", "405 AO-Demo": "No", "PixelSizeUm": 1.1, "470 AO-Demo": "No", "470 Shutter-MaxVolts": "5.0000", "Vincent-D3-Name": "Vincent-D3", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FileName": "img_000000000_P-DIC_001.tif", "LudlController-Reset": "Operate", "PRIME-Exposure": "20.00", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "445-Name": "DA Shutter", "555 Shutter-Name": "AnalogIO", "520 Shutter-TriggerSampleRate": "100000", "445 Shutter-IOChannel": "Dev1/ao9", "PRIME-TransposeCorrection": "0", "XYStage-StepSize": "0.1000", "445-Description": "DA device that is used as a shutter", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 AO-IOChannel": "Dev1/ao7", "520 Shutter-Name": "AnalogIO", "COM3-DataBits": "8", "640 AO-Volts": "0.0000", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "555 Shutter-IOChannel": "Dev1/ao13", "WaitInterval": null, "520 Shutter-Sequenceable": "1", "YPositionUm": 20690.300000000003, "445-State": "0", "445-DA Device": "445 Shutter", "PRIME-CircularBufferAutoSize": "ON", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "555 AO-TriggerSampleRate": "100000", "445 Shutter-Volts": "0.0000", "XLIGHT Hub-Port": "COM3", "Core-AutoFocus": "", "COM4-Handshaking": "Off", "528 Shutter-MinVolts": "0.0000", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "445 Shutter-Name": "AnalogIO", "528 Shutter-Sequenceable": "1", "XYStage-TransposeMirrorX": "0", "XYStage-TransposeMirrorY": "0", "PRIME-ReadoutRate": "100MHz 16bit", "XYStage-Name": "Stage", "640 AO-SupportsTriggering": "0", "640-Name": "DA Shutter", "640 Shutter-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "640 AO-Block voltage": "false", "445 Shutter-Sequenceable": "1", "470 AO-MaxVolts": "5.0000", "COM4-BaudRate": "9600", "470-Description": "DA device that is used as a shutter", "520 Shutter-Description": "NI DAC", "PRIME-CCDTemperature": "-14.9600", "Time": "2019-12-17 21:59:07 -0500", "640 AO-TriggerSequenceLength": "1000", "470 AO-Name": "AnalogIO", "XLIGHT Disk slider-Label": "Disk Pos 40um", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "Binning": "1x1", "TE2000-ModelType": "1", "COM3-AnswerTimeout": "500.0000", "555 Shutter-SupportsTriggering": "0", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "TE2000-Port": "COM4", "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Description": "NI DAC", "COM4-AnswerTimeout": "500.0000", "COM1-DelayBetweenCharsMs": "0.0000", "FilterBlock-Label": "Confocal", "445 AO-Name": "AnalogIO", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-Timing-ReadoutTimeNs": "24672000.0000", "528 AO-Demo": "No", "520 AO-Sequenceable": "1", "555-Description": "DA device that is used as a shutter", "405-DA Device": "405 Shutter", "405 AO-TriggerSequenceLength": "1000", "640 AO-MinVolts": "0.0000", "640 Shutter-Block voltage": "false", "FrameIndex": 0, "PRIME-Port": "CMOS", "555 AO-MinVolts": "0.0000", "COM3-Description": "Serial port driver (boost:asio)", "528 AO-Description": "NI DAC", "555 AO-IOChannel": "Dev1/ao6", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "555 AO-TriggerInputLine": "/Dev1/PFI0", "PRIME-ActualInterval-ms": "374.3700", "LudlController-Port": "COM9", "445 Shutter-Demo": "No", "PRIME-Trigger-Expose Out-Mux": "1", "SliceIndex": 1, "PRIME-CCDTemperatureSetPoint": "-15.0000", "405 Shutter-MinVolts": "0.0000", "PRIME-ExposeOutMode": "First Row", "AxisPositions": null, "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Volts": "0.0000", "528-Name": "DA Shutter", "555-State": "0", "XLIGHT Dichroic-Label": "Di03-R635-t1", "445 AO-Sequenceable": "1", "405-Name": "DA Shutter", "405 AO-IOChannel": "Dev1/ao0", "Core-Camera": "PRIME", "Focus-Name": "Focus", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "405 Shutter-IOChannel": "Dev1/ao8", "640 AO-Demo": "No", "640 Shutter-SupportsTriggering": "0", "445 AO-Demo": "No", "555-DA Device": "555 Shutter", "470 Shutter-Description": "NI DAC", "528 AO-Volts": "0.0000", "COM1-DataBits": "8", "Vincent-D3-Address": "x", "520 AO-SupportsTriggering": "0", "405-Description": "DA device that is used as a shutter", "640 Shutter-MinVolts": "0.0000", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "405 AO-Name": "AnalogIO", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "640 AO-TriggerInputLine": "/Dev1/PFI0", "405 AO-TriggerInputLine": "/Dev1/PFI0", "640-Description": "DA device that is used as a shutter", "445 Shutter-TriggerSampleRate": "100000", "470 Shutter-TriggerSequenceLength": "1000", "COM1-StopBits": "1", "520 Shutter-SupportsTriggering": "0", "405-State": "0", "Core-Initialize": "1", "XLIGHT Emission Wheel-State": "7", "520 AO-Description": "NI DAC", "528 AO-TriggerInputLine": "/Dev1/PFI0", "445 Shutter-MaxVolts": "5.0000", "COM9-Handshaking": "Off", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "XLIGHT Spin motor-Label": "On (spinning)", "PRIME-PP 5 ENABLED": "No", "UUID": "746bd095-57e9-4386-9818-09d602d2a0bc", "PRIME-TriggerMode": "Internal Trigger", "COM1-Handshaking": "Off", "PRIME-Timing-PostTriggerDelayNs": "49344000.0000", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "Focus-Position": "16479.8000", "528 Shutter-Block voltage": "false", "Channel": "P-DIC", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "555 Shutter-Demo": "No", "405 Shutter-Demo": "No", "528 AO-Name": "AnalogIO", "520-State": "0", "Core-Galvo": "", "405 Shutter-TriggerSampleRate": "100000", "Focus-FocusStepSize_(nm)": "100", "PRIME-PixelType": "16bit", "COM4-DataBits": "8", "470 AO-IOChannel": "Dev1/ao2", "520 AO-MinVolts": "0.0000", "COM9-Parity": "None", "PRIME-CircularBufferEnabled": "ON", "PRIME-PP 6 Reset": "No", "640 AO-Description": "NI DAC", "Source": "PRIME", "445 AO-MinVolts": "0.0000", "PIEZO-Name": "Stage", "445 AO-Description": "NI DAC", "PRIME-CircularBufferFrameCount": "46", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "520 AO-Volts": "0.0000", "528 Shutter-TriggerSampleRate": "100000", "XPositionUm": 62321, "OpticalPath-State": "4", "PRIME-PP 3 THRESHOLD": "80", "PRIME-PVCAM Version": "3.7.1", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Gain": "1-HDR", "640 Shutter-TriggerSequenceLength": "1000", "528 AO-SupportsTriggering": "0", "445 AO-TriggerInputLine": "/Dev1/PFI0", "OpticalPath-Name": "OpticalPath", "PRIME-PP 4 ENABLED": "Yes", "PRIME-PVCAM Adapter Version": "1.3.29", "COM3-Name": "COM3", "405 AO-MaxVolts": "5.0000", "520 Shutter-Block voltage": "false", "PRIME-Timing-ClearingTimeNs": "49344000.0000", "Core-Focus": "PIEZO", "528 AO-Block voltage": "false", "TE2000-Description": "Nikon TE2000 hub", "528 AO-MinVolts": "0.0000", "COM4-Parity": "None", "Summary": { "Slices": 11, "ChContrastMin": [ 0, 0, 0, 0, 0, 0 ], "PixelAspect": 1, "SlicesFirst": true, "Interval_ms": 0, "Time": "2019-12-17 21:58:39 -0500", "Prefix": "seq_3", "InitialPositionList": [ { "Label": "Pos0", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.435], "Focus": [16480.2], "XYStage": [ 69822.40000000001, 20100.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos1", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.425], "Focus": [16480.3], "XYStage": [ 69004, 20101 ] }, "GridColumnIndex": 0 }, { "Label": "Pos2", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.44], "Focus": [16480.1], "XYStage": [ 63119.5, 20690.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos3", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.405], "Focus": [16480.2], "XYStage": [ 62321, 20690.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos4", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.415], "Focus": [16480], "XYStage": [ 56604.100000000006, 20688.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos5", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.42000000000002], "Focus": [16480.1], "XYStage": [ 56034.200000000004, 20688.800000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos6", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.455], "Focus": [16480], "XYStage": [ 50889.3, 20496.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos7", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.43], "Focus": [16480.2], "XYStage": [ 50101.600000000006, 20496.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos8", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.67000000000002], "Focus": [16479.9], "XYStage": [ 44818.600000000006, 20496.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos9", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.67000000000002], "Focus": [16480], "XYStage": [ 43897.9, 20436.800000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos10", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.675], "Focus": [16479.9], "XYStage": [ 39670.600000000006, 20664.9 ] }, "GridColumnIndex": 0 }, { "Label": "Pos11", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.69], "Focus": [16479.9], "XYStage": [ 38734, 20664.600000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos12", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.695], "Focus": [16480.1], "XYStage": [ 34037.200000000004, 20664.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos13", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.685], "Focus": [16480.1], "XYStage": [ 33152, 20664.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos14", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.69], "Focus": [16480.1], "XYStage": [ 28122.800000000003, 20664.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos15", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.675], "Focus": [16480], "XYStage": [ 27422.9, 20664.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos16", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.64000000000001], "Focus": [16480], "XYStage": [ 23005.2, 20664.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos17", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.69], "Focus": [16479.9], "XYStage": [ 21820.600000000002, 20409.600000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos18", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.41], "Focus": [16480.1], "XYStage": [ 73697.6, -13222.800000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos19", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.455], "Focus": [16480.1], "XYStage": [ 72765.8, -13223 ] }, "GridColumnIndex": 0 }, { "Label": "Pos20", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.4], "Focus": [16480.2], "XYStage": [ 67445.5, -12303.800000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos21", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.415], "Focus": [16480.2], "XYStage": [ 66613.3, -12303.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos22", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.415], "Focus": [16480.1], "XYStage": [ 61819.4, -11936.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos23", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.405], "Focus": [16480.2], "XYStage": [ 61325.700000000004, -11937.1 ] }, "GridColumnIndex": 0 }, { "Label": "Pos24", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.46], "Focus": [16480.2], "XYStage": [ 55377, -10974.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos25", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.445], "Focus": [16480.1], "XYStage": [ 54827, -11214.800000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos26", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.435], "Focus": [16480.2], "XYStage": [ 49937.100000000006, -10919.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos27", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.425], "Focus": [16480.1], "XYStage": [ 48764, -10919.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos28", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.42000000000002], "Focus": [16480.2], "XYStage": [ 43978.4, -10920.400000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos29", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.435], "Focus": [16480.2], "XYStage": [ 43106.3, -10920.400000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos30", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.405], "Focus": [16480.3], "XYStage": [ 38212.4, -10920.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos31", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.445], "Focus": [16480.2], "XYStage": [ 37254.9, -10920.400000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos32", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.44], "Focus": [16480.3], "XYStage": [ 32472.800000000003, -11294.400000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos33", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [166.95000000000002], "Focus": [16480.2], "XYStage": [ 31420.7, -11294.400000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos34", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [166.95000000000002], "Focus": [16480.2], "XYStage": [ 26881.5, -11640 ] }, "GridColumnIndex": 0 }, { "Label": "Pos35", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [166.98], "Focus": [16480.2], "XYStage": [ 25876.100000000002, -11641.1 ] }, "GridColumnIndex": 0 } ], "z-step_um": 10, "GridRow": 0, "BitDepth": 16, "Depth": 2, "Channels": 6, "Source": "Micro-Manager", "ChColors": [ -16711681, -256, -65281, -1, -1, -16776961 ], "MetadataVersion": 10, "KeepShutterOpenChannels": true, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536, 65536 ], "CustomIntervals_ms": [], "Height": 1200, "MicroManagerVersion": "1.4.23 20180219", "GridColumn": 0, "UUID": "8c8f9d20-b128-4577-9f91-89e70f22d319", "PixelType": "GRAY16", "Width": 1200, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\Yan\\20191217-YC113-S5S6", "Frames": 1, "Comment": "", "UserName": "Nikon", "PixelSize_um": 1.1, "ROI": [ 0, 0, 1200, 1200 ], "Date": "2019-12-17", "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC", "P-DAPI-high" ], "KeepShutterOpenSlices": true, "IJType": 1, "ComputerName": "ZadorConfocal2", "Positions": 36, "PositionIndex": 3, "CameraTimeout": 20000, "TimeFirst": false }, "640 AO-Name": "AnalogIO", "Vincent-D3-Command": "Close" }, "FrameKey-0-4-2": { "COM4-StopBits": "1", "528 AO-MaxVolts": "5.0000", "XLIGHT Excitation Wheel-State": "2", "470 Shutter-Name": "AnalogIO", "Core-Shutter": "Vincent-D3", "555 AO-Demo": "No", "XLIGHT Dichroic-State": "2", "XYStage-StepSize-Y": "0.1000", "ZPositionUm": 147.405, "XYStage-StepSize-X": "0.1000", "PRIME-FirmwareVersion": "20.28", "PRIME-ClearCycles": "2", "520-Name": "DA Shutter", "405 Shutter-Name": "AnalogIO", "PIEZO-StepSize": "0.0050", "PRIME-ClearMode": "Pre-Exposure", "470 AO-MinVolts": "0.0000", "405 AO-SupportsTriggering": "1", "CameraChannelIndex": 0, "XYStage-StartSpeed": "500.0000", "COM9-StopBits": "2", "405 AO-Description": "NI DAC", "COM4-DelayBetweenCharsMs": "0.0000", "445 AO-Block voltage": "false", "520 AO-TriggerInputLine": "/Dev1/PFI0", "555 AO-Description": "NI DAC", "520 AO-TriggerSampleRate": "100000", "COM4-Verbose": "1", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM4-Name": "COM4", "405 Shutter-Volts": "0.0000", "Vincent-D3-Port": "COM1", "PRIME-PP 4 MIN ADU AFFECTED": "200", "470 Shutter-MinVolts": "0.0000", "PRIME-PP 3 ENABLED": "Yes", "470 AO-Description": "NI DAC", "PRIME-Description": "PVCAM API device adapter", "520 Shutter-MaxVolts": "5.0000", "520 Shutter-Volts": "0.0000", "528 AO-Sequenceable": "1", "PRIME-Binning": "1x1", "555 Shutter-MaxVolts": "5.0000", "528 Shutter-TriggerSequenceLength": "1000", "405 Shutter-Block voltage": "false", "XLIGHT Spin motor-State": "1", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "Vincent-D3-Time to open (ms)": "35.0000", "Vincent-D3-Time to close (ms)": "35.0000", "445 AO-TriggerSampleRate": "100000", "640 Shutter-IOChannel": "Dev1/ao14", "555 AO-Sequenceable": "1", "520 AO-Name": "AnalogIO", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "470 AO-Block voltage": "false", "COM1-Verbose": "1", "COM9-AnswerTimeout": "10000.0000", "528 Shutter-Name": "AnalogIO", "PRIME-Trigger Timeout (secs)": "10", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "NextFrame": 0, "470 AO-TriggerSequenceLength": "1000", "PRIME-TransposeXY": "0", "445 AO-TriggerSequenceLength": "1000", "COM1-Name": "COM1", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "COM9-DataBits": "8", "COM1-AnswerTimeout": "500.0000", "Camera": "", "TE2000-Version": "V2.50-V2.50-V2.10", "528 Shutter-Volts": "0.0000", "XLIGHT Hub-Name": "XLIGHT Hub", "PIEZO-Description": "Ludl stage driver adapter", "PositionName": "Pos3", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSampleRate": "100000", "PRIME-FanSpeedSetpoint": "High", "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "405 AO-TriggerSampleRate": "100000", "470 Shutter-Block voltage": "false", "470-State": "0", "COM3-Parity": "None", "405 AO-Volts": "0.0000", "PRIME-TransposeMirrorY": "0", "PRIME-ChipName": "GS144BSI", "470 AO-Volts": "0.0000", "PRIME-TransposeMirrorX": "0", "445 Shutter-SupportsTriggering": "0", "470 Shutter-SupportsTriggering": "0", "XLIGHT Hub-Description": "XLIGHT hub", "PRIME-PreampOffLimit": "10000", "470 AO-Sequenceable": "1", "528 Shutter-MaxVolts": "5.0000", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "Core-XYStage": "XYStage", "470 AO-TriggerSampleRate": "100000", "PRIME-Timing-PreTriggerDelayNs": "24672000.0000", "528-DA Device": "528 Shutter", "Focus-Description": "Nikon TE2000 focus adapter", "FilterBlock-State": "5", "520 AO-Demo": "No", "COM3-DelayBetweenCharsMs": "0.0000", "470 Shutter-Volts": "0.0000", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "PRIME-PP 2 THRESHOLD": "125", "PRIME-PP 4 THRESHOLD": "75", "XYStage-Acceleration": "4.0000", "470 Shutter-Demo": "No", "PRIME-Name": "PMPCIECam00", "640-DA Device": "640 Shutter", "405 AO-Block voltage": "false", "ElapsedTime-ms": 160445, "PRIME-Offset": "100", "445 AO-IOChannel": "Dev1/ao1", "640 AO-MaxVolts": "5.0000", "PositionIndex": 3, "PRIME-PP 1 ENABLED": "Yes", "COM1-BaudRate": "9600", "640-State": "0", "COM3-Verbose": "1", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "LudlController-TransmissionDelay": "0", "PRIME-FullWellCapacity": "62000", "PRIME-SMARTStreamingEnabled": "No", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "640 Shutter-Description": "NI DAC", "528-Description": "DA device that is used as a shutter", "COM4-Description": "Serial port driver (boost:asio)", "405 Shutter-Description": "NI DAC", "TE2000-Name": "TE2000", "528 Shutter-Description": "NI DAC", "COM9-Description": "Serial port driver (boost:asio)", "LudlController-Name": "LudlController", "445 Shutter-TriggerSequenceLength": "1000", "PRIME-SerialNumber": "A18B203008", "445 AO-Volts": "0.0000", "PRIME-X-dimension": "1200", "PRIME-ShutterCloseDelay": "0", "XLIGHT Disk slider-State": "2", "Position": "Default", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 AO-IOChannel": "Dev1/ao5", "520 AO-IOChannel": "Dev1/ao4", "528 AO-TriggerSequenceLength": "1000", "405 Shutter-MaxVolts": "5.0000", "470-DA Device": "470 Shutter", "PRIME-FTCapable": "No", "Core-ChannelGroup": "Channels", "555 AO-SupportsTriggering": "0", "PRIME-Y-dimension": "1200", "Frame": 0, "555 Shutter-Sequenceable": "1", "PRIME-Timing-ExposureTimeNs": "9992000.0000", "Core-ImageProcessor": "", "528-State": "0", "520 AO-Block voltage": "false", "XLIGHT Excitation Wheel-Label": "Excitation-2", "640 Shutter-Demo": "No", "COM3-StopBits": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "520 Shutter-IOChannel": "Dev1/ao11", "Width": 1200, "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-SupportsTriggering": "0", "640 AO-TriggerSampleRate": "100000", "520-Description": "DA device that is used as a shutter", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "405 Shutter-Sequenceable": "1", "405 Shutter-SupportsTriggering": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-TriggerSampleRate": "100000", "640 Shutter-MaxVolts": "5.0000", "555 AO-MaxVolts": "5.0000", "520 AO-TriggerSequenceLength": "1000", "COM3-BaudRate": "9600", "COM9-BaudRate": "9600", "Vincent-D3-Channel #": "Ch. #1", "PRIME-PMode": "Normal", "OpticalPath-Label": "Left(100)", "BitDepth": 16, "555 AO-TriggerSequenceLength": "1000", "445 Shutter-Description": "NI DAC", "405 AO-Sequenceable": "1", "PRIME-AcquisitionMethod": "Callbacks", "555 AO-Name": "AnalogIO", "PRIME-ShutterMode": "Pre-Exposure", "COM3-Handshaking": "Off", "445 AO-SupportsTriggering": "0", "ROI": "0-0-1200-1200", "COM9-Verbose": "1", "PRIME-Color": "OFF", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "PRIME-ShutterOpenDelay": "0", "COM1-Parity": "None", "Slice": 2, "SlicePosition": -30, "COM9-DelayBetweenCharsMs": "0.0000", "555-Name": "DA Shutter", "PIEZO-LudlSingleAxisName": "B", "XLIGHT Emission Wheel-Label": "Empty", "640 Shutter-Name": "AnalogIO", "640 AO-Sequenceable": "1", "520 Shutter-Demo": "No", "528 Shutter-Demo": "No", "470-Name": "DA Shutter", "520-DA Device": "520 Shutter", "XYStage-Description": "Ludl XY stage driver adapter", "445 Shutter-MinVolts": "0.0000", "528 AO-TriggerSampleRate": "100000", "405 AO-MinVolts": "0.0000", "405 Shutter-TriggerSequenceLength": "1000", "Height": 1200, "555 AO-Block voltage": "false", "640 Shutter-Volts": "0.0000", "PixelType": "GRAY16", "445 Shutter-Block voltage": "false", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "520 Shutter-TriggerSequenceLength": "1000", "COM9-Name": "COM9", "Core-SLM": "", "555 AO-Volts": "0.0000", "555 Shutter-Block voltage": "false", "XYStage-Speed": "1800.0000", "520 AO-MaxVolts": "5.0000", "528 Shutter-SupportsTriggering": "0", "COM1-Description": "Serial port driver (boost:asio)", "PRIME-MetadataEnabled": "No", "520 Shutter-MinVolts": "0.0000", "470 Shutter-Sequenceable": "1", "PRIME-PreampDelay": "0", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-MinVolts": "0.0000", "ChannelIndex": 4, "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Core-AutoShutter": "1", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "Exposure-ms": 20, "PRIME-PP 2 ENABLED": "Yes", "640 Shutter-TriggerSampleRate": "100000", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "470 Shutter-IOChannel": "Dev1/ao10", "405 AO-Demo": "No", "PixelSizeUm": 1.1, "470 AO-Demo": "No", "470 Shutter-MaxVolts": "5.0000", "Vincent-D3-Name": "Vincent-D3", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FileName": "img_000000000_P-DIC_002.tif", "LudlController-Reset": "Operate", "PRIME-Exposure": "20.00", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "445-Name": "DA Shutter", "555 Shutter-Name": "AnalogIO", "520 Shutter-TriggerSampleRate": "100000", "445 Shutter-IOChannel": "Dev1/ao9", "PRIME-TransposeCorrection": "0", "XYStage-StepSize": "0.1000", "445-Description": "DA device that is used as a shutter", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 AO-IOChannel": "Dev1/ao7", "520 Shutter-Name": "AnalogIO", "COM3-DataBits": "8", "640 AO-Volts": "0.0000", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "555 Shutter-IOChannel": "Dev1/ao13", "WaitInterval": null, "520 Shutter-Sequenceable": "1", "YPositionUm": 20690.300000000003, "445-State": "0", "445-DA Device": "445 Shutter", "PRIME-CircularBufferAutoSize": "ON", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "555 AO-TriggerSampleRate": "100000", "445 Shutter-Volts": "0.0000", "XLIGHT Hub-Port": "COM3", "Core-AutoFocus": "", "COM4-Handshaking": "Off", "528 Shutter-MinVolts": "0.0000", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "445 Shutter-Name": "AnalogIO", "528 Shutter-Sequenceable": "1", "XYStage-TransposeMirrorX": "0", "XYStage-TransposeMirrorY": "0", "PRIME-ReadoutRate": "100MHz 16bit", "XYStage-Name": "Stage", "640 AO-SupportsTriggering": "0", "640-Name": "DA Shutter", "640 Shutter-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "640 AO-Block voltage": "false", "445 Shutter-Sequenceable": "1", "470 AO-MaxVolts": "5.0000", "COM4-BaudRate": "9600", "470-Description": "DA device that is used as a shutter", "520 Shutter-Description": "NI DAC", "PRIME-CCDTemperature": "-14.9600", "Time": "2019-12-17 21:59:07 -0500", "640 AO-TriggerSequenceLength": "1000", "470 AO-Name": "AnalogIO", "XLIGHT Disk slider-Label": "Disk Pos 40um", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "Binning": "1x1", "TE2000-ModelType": "1", "COM3-AnswerTimeout": "500.0000", "555 Shutter-SupportsTriggering": "0", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "TE2000-Port": "COM4", "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Description": "NI DAC", "COM4-AnswerTimeout": "500.0000", "COM1-DelayBetweenCharsMs": "0.0000", "FilterBlock-Label": "Confocal", "445 AO-Name": "AnalogIO", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-Timing-ReadoutTimeNs": "24672000.0000", "528 AO-Demo": "No", "520 AO-Sequenceable": "1", "555-Description": "DA device that is used as a shutter", "405-DA Device": "405 Shutter", "405 AO-TriggerSequenceLength": "1000", "640 AO-MinVolts": "0.0000", "640 Shutter-Block voltage": "false", "FrameIndex": 0, "PRIME-Port": "CMOS", "555 AO-MinVolts": "0.0000", "COM3-Description": "Serial port driver (boost:asio)", "528 AO-Description": "NI DAC", "555 AO-IOChannel": "Dev1/ao6", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "555 AO-TriggerInputLine": "/Dev1/PFI0", "PRIME-ActualInterval-ms": "374.3700", "LudlController-Port": "COM9", "445 Shutter-Demo": "No", "PRIME-Trigger-Expose Out-Mux": "1", "SliceIndex": 2, "PRIME-CCDTemperatureSetPoint": "-15.0000", "405 Shutter-MinVolts": "0.0000", "PRIME-ExposeOutMode": "First Row", "AxisPositions": null, "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Volts": "0.0000", "528-Name": "DA Shutter", "555-State": "0", "XLIGHT Dichroic-Label": "Di03-R635-t1", "445 AO-Sequenceable": "1", "405-Name": "DA Shutter", "405 AO-IOChannel": "Dev1/ao0", "Core-Camera": "PRIME", "Focus-Name": "Focus", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "405 Shutter-IOChannel": "Dev1/ao8", "640 AO-Demo": "No", "640 Shutter-SupportsTriggering": "0", "445 AO-Demo": "No", "555-DA Device": "555 Shutter", "470 Shutter-Description": "NI DAC", "528 AO-Volts": "0.0000", "COM1-DataBits": "8", "Vincent-D3-Address": "x", "520 AO-SupportsTriggering": "0", "405-Description": "DA device that is used as a shutter", "640 Shutter-MinVolts": "0.0000", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "405 AO-Name": "AnalogIO", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "640 AO-TriggerInputLine": "/Dev1/PFI0", "405 AO-TriggerInputLine": "/Dev1/PFI0", "640-Description": "DA device that is used as a shutter", "445 Shutter-TriggerSampleRate": "100000", "470 Shutter-TriggerSequenceLength": "1000", "COM1-StopBits": "1", "520 Shutter-SupportsTriggering": "0", "405-State": "0", "Core-Initialize": "1", "XLIGHT Emission Wheel-State": "7", "520 AO-Description": "NI DAC", "528 AO-TriggerInputLine": "/Dev1/PFI0", "445 Shutter-MaxVolts": "5.0000", "COM9-Handshaking": "Off", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "XLIGHT Spin motor-Label": "On (spinning)", "PRIME-PP 5 ENABLED": "No", "UUID": "dbbb66e0-bcff-46ca-bd67-cd4a20e8ea06", "PRIME-TriggerMode": "Internal Trigger", "COM1-Handshaking": "Off", "PRIME-Timing-PostTriggerDelayNs": "49344000.0000", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "Focus-Position": "16479.8000", "528 Shutter-Block voltage": "false", "Channel": "P-DIC", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "555 Shutter-Demo": "No", "405 Shutter-Demo": "No", "528 AO-Name": "AnalogIO", "520-State": "0", "Core-Galvo": "", "405 Shutter-TriggerSampleRate": "100000", "Focus-FocusStepSize_(nm)": "100", "PRIME-PixelType": "16bit", "COM4-DataBits": "8", "470 AO-IOChannel": "Dev1/ao2", "520 AO-MinVolts": "0.0000", "COM9-Parity": "None", "PRIME-CircularBufferEnabled": "ON", "PRIME-PP 6 Reset": "No", "640 AO-Description": "NI DAC", "Source": "PRIME", "445 AO-MinVolts": "0.0000", "PIEZO-Name": "Stage", "445 AO-Description": "NI DAC", "PRIME-CircularBufferFrameCount": "46", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "520 AO-Volts": "0.0000", "528 Shutter-TriggerSampleRate": "100000", "XPositionUm": 62321, "OpticalPath-State": "4", "PRIME-PP 3 THRESHOLD": "80", "PRIME-PVCAM Version": "3.7.1", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Gain": "1-HDR", "640 Shutter-TriggerSequenceLength": "1000", "528 AO-SupportsTriggering": "0", "445 AO-TriggerInputLine": "/Dev1/PFI0", "OpticalPath-Name": "OpticalPath", "PRIME-PP 4 ENABLED": "Yes", "PRIME-PVCAM Adapter Version": "1.3.29", "COM3-Name": "COM3", "405 AO-MaxVolts": "5.0000", "520 Shutter-Block voltage": "false", "PRIME-Timing-ClearingTimeNs": "49344000.0000", "Core-Focus": "PIEZO", "528 AO-Block voltage": "false", "TE2000-Description": "Nikon TE2000 hub", "528 AO-MinVolts": "0.0000", "COM4-Parity": "None", "Summary": { "Slices": 11, "ChContrastMin": [ 0, 0, 0, 0, 0, 0 ], "PixelAspect": 1, "SlicesFirst": true, "Interval_ms": 0, "Time": "2019-12-17 21:58:39 -0500", "Prefix": "seq_3", "InitialPositionList": [ { "Label": "Pos0", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.435], "Focus": [16480.2], "XYStage": [ 69822.40000000001, 20100.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos1", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.425], "Focus": [16480.3], "XYStage": [ 69004, 20101 ] }, "GridColumnIndex": 0 }, { "Label": "Pos2", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.44], "Focus": [16480.1], "XYStage": [ 63119.5, 20690.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos3", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.405], "Focus": [16480.2], "XYStage": [ 62321, 20690.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos4", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.415], "Focus": [16480], "XYStage": [ 56604.100000000006, 20688.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos5", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.42000000000002], "Focus": [16480.1], "XYStage": [ 56034.200000000004, 20688.800000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos6", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.455], "Focus": [16480], "XYStage": [ 50889.3, 20496.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos7", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.43], "Focus": [16480.2], "XYStage": [ 50101.600000000006, 20496.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos8", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.67000000000002], "Focus": [16479.9], "XYStage": [ 44818.600000000006, 20496.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos9", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.67000000000002], "Focus": [16480], "XYStage": [ 43897.9, 20436.800000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos10", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.675], "Focus": [16479.9], "XYStage": [ 39670.600000000006, 20664.9 ] }, "GridColumnIndex": 0 }, { "Label": "Pos11", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.69], "Focus": [16479.9], "XYStage": [ 38734, 20664.600000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos12", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.695], "Focus": [16480.1], "XYStage": [ 34037.200000000004, 20664.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos13", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.685], "Focus": [16480.1], "XYStage": [ 33152, 20664.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos14", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.69], "Focus": [16480.1], "XYStage": [ 28122.800000000003, 20664.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos15", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.675], "Focus": [16480], "XYStage": [ 27422.9, 20664.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos16", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.64000000000001], "Focus": [16480], "XYStage": [ 23005.2, 20664.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos17", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.69], "Focus": [16479.9], "XYStage": [ 21820.600000000002, 20409.600000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos18", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.41], "Focus": [16480.1], "XYStage": [ 73697.6, -13222.800000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos19", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.455], "Focus": [16480.1], "XYStage": [ 72765.8, -13223 ] }, "GridColumnIndex": 0 }, { "Label": "Pos20", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.4], "Focus": [16480.2], "XYStage": [ 67445.5, -12303.800000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos21", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.415], "Focus": [16480.2], "XYStage": [ 66613.3, -12303.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos22", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.415], "Focus": [16480.1], "XYStage": [ 61819.4, -11936.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos23", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.405], "Focus": [16480.2], "XYStage": [ 61325.700000000004, -11937.1 ] }, "GridColumnIndex": 0 }, { "Label": "Pos24", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.46], "Focus": [16480.2], "XYStage": [ 55377, -10974.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos25", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.445], "Focus": [16480.1], "XYStage": [ 54827, -11214.800000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos26", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.435], "Focus": [16480.2], "XYStage": [ 49937.100000000006, -10919.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos27", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.425], "Focus": [16480.1], "XYStage": [ 48764, -10919.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos28", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.42000000000002], "Focus": [16480.2], "XYStage": [ 43978.4, -10920.400000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos29", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.435], "Focus": [16480.2], "XYStage": [ 43106.3, -10920.400000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos30", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.405], "Focus": [16480.3], "XYStage": [ 38212.4, -10920.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos31", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.445], "Focus": [16480.2], "XYStage": [ 37254.9, -10920.400000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos32", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.44], "Focus": [16480.3], "XYStage": [ 32472.800000000003, -11294.400000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos33", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [166.95000000000002], "Focus": [16480.2], "XYStage": [ 31420.7, -11294.400000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos34", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [166.95000000000002], "Focus": [16480.2], "XYStage": [ 26881.5, -11640 ] }, "GridColumnIndex": 0 }, { "Label": "Pos35", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [166.98], "Focus": [16480.2], "XYStage": [ 25876.100000000002, -11641.1 ] }, "GridColumnIndex": 0 } ], "z-step_um": 10, "GridRow": 0, "BitDepth": 16, "Depth": 2, "Channels": 6, "Source": "Micro-Manager", "ChColors": [ -16711681, -256, -65281, -1, -1, -16776961 ], "MetadataVersion": 10, "KeepShutterOpenChannels": true, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536, 65536 ], "CustomIntervals_ms": [], "Height": 1200, "MicroManagerVersion": "1.4.23 20180219", "GridColumn": 0, "UUID": "8c8f9d20-b128-4577-9f91-89e70f22d319", "PixelType": "GRAY16", "Width": 1200, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\Yan\\20191217-YC113-S5S6", "Frames": 1, "Comment": "", "UserName": "Nikon", "PixelSize_um": 1.1, "ROI": [ 0, 0, 1200, 1200 ], "Date": "2019-12-17", "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC", "P-DAPI-high" ], "KeepShutterOpenSlices": true, "IJType": 1, "ComputerName": "ZadorConfocal2", "Positions": 36, "PositionIndex": 3, "CameraTimeout": 20000, "TimeFirst": false }, "640 AO-Name": "AnalogIO", "Vincent-D3-Command": "Close" }, "FrameKey-0-4-3": { "COM4-StopBits": "1", "528 AO-MaxVolts": "5.0000", "XLIGHT Excitation Wheel-State": "2", "470 Shutter-Name": "AnalogIO", "Core-Shutter": "Vincent-D3", "555 AO-Demo": "No", "XLIGHT Dichroic-State": "2", "XYStage-StepSize-Y": "0.1000", "ZPositionUm": 157.405, "XYStage-StepSize-X": "0.1000", "PRIME-FirmwareVersion": "20.28", "PRIME-ClearCycles": "2", "520-Name": "DA Shutter", "405 Shutter-Name": "AnalogIO", "PIEZO-StepSize": "0.0050", "PRIME-ClearMode": "Pre-Exposure", "470 AO-MinVolts": "0.0000", "405 AO-SupportsTriggering": "1", "CameraChannelIndex": 0, "XYStage-StartSpeed": "500.0000", "COM9-StopBits": "2", "405 AO-Description": "NI DAC", "COM4-DelayBetweenCharsMs": "0.0000", "445 AO-Block voltage": "false", "520 AO-TriggerInputLine": "/Dev1/PFI0", "555 AO-Description": "NI DAC", "520 AO-TriggerSampleRate": "100000", "COM4-Verbose": "1", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM4-Name": "COM4", "405 Shutter-Volts": "0.0000", "Vincent-D3-Port": "COM1", "PRIME-PP 4 MIN ADU AFFECTED": "200", "470 Shutter-MinVolts": "0.0000", "PRIME-PP 3 ENABLED": "Yes", "470 AO-Description": "NI DAC", "PRIME-Description": "PVCAM API device adapter", "520 Shutter-MaxVolts": "5.0000", "520 Shutter-Volts": "0.0000", "528 AO-Sequenceable": "1", "PRIME-Binning": "1x1", "555 Shutter-MaxVolts": "5.0000", "528 Shutter-TriggerSequenceLength": "1000", "405 Shutter-Block voltage": "false", "XLIGHT Spin motor-State": "1", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "Vincent-D3-Time to open (ms)": "35.0000", "Vincent-D3-Time to close (ms)": "35.0000", "445 AO-TriggerSampleRate": "100000", "640 Shutter-IOChannel": "Dev1/ao14", "555 AO-Sequenceable": "1", "520 AO-Name": "AnalogIO", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "470 AO-Block voltage": "false", "COM1-Verbose": "1", "COM9-AnswerTimeout": "10000.0000", "528 Shutter-Name": "AnalogIO", "PRIME-Trigger Timeout (secs)": "10", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "NextFrame": 0, "470 AO-TriggerSequenceLength": "1000", "PRIME-TransposeXY": "0", "445 AO-TriggerSequenceLength": "1000", "COM1-Name": "COM1", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "COM9-DataBits": "8", "COM1-AnswerTimeout": "500.0000", "Camera": "", "TE2000-Version": "V2.50-V2.50-V2.10", "528 Shutter-Volts": "0.0000", "XLIGHT Hub-Name": "XLIGHT Hub", "PIEZO-Description": "Ludl stage driver adapter", "PositionName": "Pos3", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSampleRate": "100000", "PRIME-FanSpeedSetpoint": "High", "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "405 AO-TriggerSampleRate": "100000", "470 Shutter-Block voltage": "false", "470-State": "0", "COM3-Parity": "None", "405 AO-Volts": "0.0000", "PRIME-TransposeMirrorY": "0", "PRIME-ChipName": "GS144BSI", "470 AO-Volts": "0.0000", "PRIME-TransposeMirrorX": "0", "445 Shutter-SupportsTriggering": "0", "470 Shutter-SupportsTriggering": "0", "XLIGHT Hub-Description": "XLIGHT hub", "PRIME-PreampOffLimit": "10000", "470 AO-Sequenceable": "1", "528 Shutter-MaxVolts": "5.0000", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "Core-XYStage": "XYStage", "470 AO-TriggerSampleRate": "100000", "PRIME-Timing-PreTriggerDelayNs": "24672000.0000", "528-DA Device": "528 Shutter", "Focus-Description": "Nikon TE2000 focus adapter", "FilterBlock-State": "5", "520 AO-Demo": "No", "COM3-DelayBetweenCharsMs": "0.0000", "470 Shutter-Volts": "0.0000", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "PRIME-PP 2 THRESHOLD": "125", "PRIME-PP 4 THRESHOLD": "75", "XYStage-Acceleration": "4.0000", "470 Shutter-Demo": "No", "PRIME-Name": "PMPCIECam00", "640-DA Device": "640 Shutter", "405 AO-Block voltage": "false", "ElapsedTime-ms": 160713, "PRIME-Offset": "100", "445 AO-IOChannel": "Dev1/ao1", "640 AO-MaxVolts": "5.0000", "PositionIndex": 3, "PRIME-PP 1 ENABLED": "Yes", "COM1-BaudRate": "9600", "640-State": "0", "COM3-Verbose": "1", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "LudlController-TransmissionDelay": "0", "PRIME-FullWellCapacity": "62000", "PRIME-SMARTStreamingEnabled": "No", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "640 Shutter-Description": "NI DAC", "528-Description": "DA device that is used as a shutter", "COM4-Description": "Serial port driver (boost:asio)", "405 Shutter-Description": "NI DAC", "TE2000-Name": "TE2000", "528 Shutter-Description": "NI DAC", "COM9-Description": "Serial port driver (boost:asio)", "LudlController-Name": "LudlController", "445 Shutter-TriggerSequenceLength": "1000", "PRIME-SerialNumber": "A18B203008", "445 AO-Volts": "0.0000", "PRIME-X-dimension": "1200", "PRIME-ShutterCloseDelay": "0", "XLIGHT Disk slider-State": "2", "Position": "Default", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 AO-IOChannel": "Dev1/ao5", "520 AO-IOChannel": "Dev1/ao4", "528 AO-TriggerSequenceLength": "1000", "405 Shutter-MaxVolts": "5.0000", "470-DA Device": "470 Shutter", "PRIME-FTCapable": "No", "Core-ChannelGroup": "Channels", "555 AO-SupportsTriggering": "0", "PRIME-Y-dimension": "1200", "Frame": 0, "555 Shutter-Sequenceable": "1", "PRIME-Timing-ExposureTimeNs": "9992000.0000", "Core-ImageProcessor": "", "528-State": "0", "520 AO-Block voltage": "false", "XLIGHT Excitation Wheel-Label": "Excitation-2", "640 Shutter-Demo": "No", "COM3-StopBits": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "520 Shutter-IOChannel": "Dev1/ao11", "Width": 1200, "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-SupportsTriggering": "0", "640 AO-TriggerSampleRate": "100000", "520-Description": "DA device that is used as a shutter", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "405 Shutter-Sequenceable": "1", "405 Shutter-SupportsTriggering": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-TriggerSampleRate": "100000", "640 Shutter-MaxVolts": "5.0000", "555 AO-MaxVolts": "5.0000", "520 AO-TriggerSequenceLength": "1000", "COM3-BaudRate": "9600", "COM9-BaudRate": "9600", "Vincent-D3-Channel #": "Ch. #1", "PRIME-PMode": "Normal", "OpticalPath-Label": "Left(100)", "BitDepth": 16, "555 AO-TriggerSequenceLength": "1000", "445 Shutter-Description": "NI DAC", "405 AO-Sequenceable": "1", "PRIME-AcquisitionMethod": "Callbacks", "555 AO-Name": "AnalogIO", "PRIME-ShutterMode": "Pre-Exposure", "COM3-Handshaking": "Off", "445 AO-SupportsTriggering": "0", "ROI": "0-0-1200-1200", "COM9-Verbose": "1", "PRIME-Color": "OFF", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "PRIME-ShutterOpenDelay": "0", "COM1-Parity": "None", "Slice": 3, "SlicePosition": -20, "COM9-DelayBetweenCharsMs": "0.0000", "555-Name": "DA Shutter", "PIEZO-LudlSingleAxisName": "B", "XLIGHT Emission Wheel-Label": "Empty", "640 Shutter-Name": "AnalogIO", "640 AO-Sequenceable": "1", "520 Shutter-Demo": "No", "528 Shutter-Demo": "No", "470-Name": "DA Shutter", "520-DA Device": "520 Shutter", "XYStage-Description": "Ludl XY stage driver adapter", "445 Shutter-MinVolts": "0.0000", "528 AO-TriggerSampleRate": "100000", "405 AO-MinVolts": "0.0000", "405 Shutter-TriggerSequenceLength": "1000", "Height": 1200, "555 AO-Block voltage": "false", "640 Shutter-Volts": "0.0000", "PixelType": "GRAY16", "445 Shutter-Block voltage": "false", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "520 Shutter-TriggerSequenceLength": "1000", "COM9-Name": "COM9", "Core-SLM": "", "555 AO-Volts": "0.0000", "555 Shutter-Block voltage": "false", "XYStage-Speed": "1800.0000", "520 AO-MaxVolts": "5.0000", "528 Shutter-SupportsTriggering": "0", "COM1-Description": "Serial port driver (boost:asio)", "PRIME-MetadataEnabled": "No", "520 Shutter-MinVolts": "0.0000", "470 Shutter-Sequenceable": "1", "PRIME-PreampDelay": "0", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-MinVolts": "0.0000", "ChannelIndex": 4, "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Core-AutoShutter": "1", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "Exposure-ms": 20, "PRIME-PP 2 ENABLED": "Yes", "640 Shutter-TriggerSampleRate": "100000", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "470 Shutter-IOChannel": "Dev1/ao10", "405 AO-Demo": "No", "PixelSizeUm": 1.1, "470 AO-Demo": "No", "470 Shutter-MaxVolts": "5.0000", "Vincent-D3-Name": "Vincent-D3", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FileName": "img_000000000_P-DIC_003.tif", "LudlController-Reset": "Operate", "PRIME-Exposure": "20.00", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "445-Name": "DA Shutter", "555 Shutter-Name": "AnalogIO", "520 Shutter-TriggerSampleRate": "100000", "445 Shutter-IOChannel": "Dev1/ao9", "PRIME-TransposeCorrection": "0", "XYStage-StepSize": "0.1000", "445-Description": "DA device that is used as a shutter", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 AO-IOChannel": "Dev1/ao7", "520 Shutter-Name": "AnalogIO", "COM3-DataBits": "8", "640 AO-Volts": "0.0000", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "555 Shutter-IOChannel": "Dev1/ao13", "WaitInterval": null, "520 Shutter-Sequenceable": "1", "YPositionUm": 20690.300000000003, "445-State": "0", "445-DA Device": "445 Shutter", "PRIME-CircularBufferAutoSize": "ON", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "555 AO-TriggerSampleRate": "100000", "445 Shutter-Volts": "0.0000", "XLIGHT Hub-Port": "COM3", "Core-AutoFocus": "", "COM4-Handshaking": "Off", "528 Shutter-MinVolts": "0.0000", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "445 Shutter-Name": "AnalogIO", "528 Shutter-Sequenceable": "1", "XYStage-TransposeMirrorX": "0", "XYStage-TransposeMirrorY": "0", "PRIME-ReadoutRate": "100MHz 16bit", "XYStage-Name": "Stage", "640 AO-SupportsTriggering": "0", "640-Name": "DA Shutter", "640 Shutter-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "640 AO-Block voltage": "false", "445 Shutter-Sequenceable": "1", "470 AO-MaxVolts": "5.0000", "COM4-BaudRate": "9600", "470-Description": "DA device that is used as a shutter", "520 Shutter-Description": "NI DAC", "PRIME-CCDTemperature": "-14.9600", "Time": "2019-12-17 21:59:08 -0500", "640 AO-TriggerSequenceLength": "1000", "470 AO-Name": "AnalogIO", "XLIGHT Disk slider-Label": "Disk Pos 40um", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "Binning": "1x1", "TE2000-ModelType": "1", "COM3-AnswerTimeout": "500.0000", "555 Shutter-SupportsTriggering": "0", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "TE2000-Port": "COM4", "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Description": "NI DAC", "COM4-AnswerTimeout": "500.0000", "COM1-DelayBetweenCharsMs": "0.0000", "FilterBlock-Label": "Confocal", "445 AO-Name": "AnalogIO", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-Timing-ReadoutTimeNs": "24672000.0000", "528 AO-Demo": "No", "520 AO-Sequenceable": "1", "555-Description": "DA device that is used as a shutter", "405-DA Device": "405 Shutter", "405 AO-TriggerSequenceLength": "1000", "640 AO-MinVolts": "0.0000", "640 Shutter-Block voltage": "false", "FrameIndex": 0, "PRIME-Port": "CMOS", "555 AO-MinVolts": "0.0000", "COM3-Description": "Serial port driver (boost:asio)", "528 AO-Description": "NI DAC", "555 AO-IOChannel": "Dev1/ao6", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "555 AO-TriggerInputLine": "/Dev1/PFI0", "PRIME-ActualInterval-ms": "374.3700", "LudlController-Port": "COM9", "445 Shutter-Demo": "No", "PRIME-Trigger-Expose Out-Mux": "1", "SliceIndex": 3, "PRIME-CCDTemperatureSetPoint": "-15.0000", "405 Shutter-MinVolts": "0.0000", "PRIME-ExposeOutMode": "First Row", "AxisPositions": null, "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Volts": "0.0000", "528-Name": "DA Shutter", "555-State": "0", "XLIGHT Dichroic-Label": "Di03-R635-t1", "445 AO-Sequenceable": "1", "405-Name": "DA Shutter", "405 AO-IOChannel": "Dev1/ao0", "Core-Camera": "PRIME", "Focus-Name": "Focus", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "405 Shutter-IOChannel": "Dev1/ao8", "640 AO-Demo": "No", "640 Shutter-SupportsTriggering": "0", "445 AO-Demo": "No", "555-DA Device": "555 Shutter", "470 Shutter-Description": "NI DAC", "528 AO-Volts": "0.0000", "COM1-DataBits": "8", "Vincent-D3-Address": "x", "520 AO-SupportsTriggering": "0", "405-Description": "DA device that is used as a shutter", "640 Shutter-MinVolts": "0.0000", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "405 AO-Name": "AnalogIO", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "640 AO-TriggerInputLine": "/Dev1/PFI0", "405 AO-TriggerInputLine": "/Dev1/PFI0", "640-Description": "DA device that is used as a shutter", "445 Shutter-TriggerSampleRate": "100000", "470 Shutter-TriggerSequenceLength": "1000", "COM1-StopBits": "1", "520 Shutter-SupportsTriggering": "0", "405-State": "0", "Core-Initialize": "1", "XLIGHT Emission Wheel-State": "7", "520 AO-Description": "NI DAC", "528 AO-TriggerInputLine": "/Dev1/PFI0", "445 Shutter-MaxVolts": "5.0000", "COM9-Handshaking": "Off", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "XLIGHT Spin motor-Label": "On (spinning)", "PRIME-PP 5 ENABLED": "No", "UUID": "ad60309d-e5ef-4a46-af37-841917b9b73d", "PRIME-TriggerMode": "Internal Trigger", "COM1-Handshaking": "Off", "PRIME-Timing-PostTriggerDelayNs": "49344000.0000", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "Focus-Position": "16479.8000", "528 Shutter-Block voltage": "false", "Channel": "P-DIC", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "555 Shutter-Demo": "No", "405 Shutter-Demo": "No", "528 AO-Name": "AnalogIO", "520-State": "0", "Core-Galvo": "", "405 Shutter-TriggerSampleRate": "100000", "Focus-FocusStepSize_(nm)": "100", "PRIME-PixelType": "16bit", "COM4-DataBits": "8", "470 AO-IOChannel": "Dev1/ao2", "520 AO-MinVolts": "0.0000", "COM9-Parity": "None", "PRIME-CircularBufferEnabled": "ON", "PRIME-PP 6 Reset": "No", "640 AO-Description": "NI DAC", "Source": "PRIME", "445 AO-MinVolts": "0.0000", "PIEZO-Name": "Stage", "445 AO-Description": "NI DAC", "PRIME-CircularBufferFrameCount": "46", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "520 AO-Volts": "0.0000", "528 Shutter-TriggerSampleRate": "100000", "XPositionUm": 62321, "OpticalPath-State": "4", "PRIME-PP 3 THRESHOLD": "80", "PRIME-PVCAM Version": "3.7.1", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Gain": "1-HDR", "640 Shutter-TriggerSequenceLength": "1000", "528 AO-SupportsTriggering": "0", "445 AO-TriggerInputLine": "/Dev1/PFI0", "OpticalPath-Name": "OpticalPath", "PRIME-PP 4 ENABLED": "Yes", "PRIME-PVCAM Adapter Version": "1.3.29", "COM3-Name": "COM3", "405 AO-MaxVolts": "5.0000", "520 Shutter-Block voltage": "false", "PRIME-Timing-ClearingTimeNs": "49344000.0000", "Core-Focus": "PIEZO", "528 AO-Block voltage": "false", "TE2000-Description": "Nikon TE2000 hub", "528 AO-MinVolts": "0.0000", "COM4-Parity": "None", "Summary": { "Slices": 11, "ChContrastMin": [ 0, 0, 0, 0, 0, 0 ], "PixelAspect": 1, "SlicesFirst": true, "Interval_ms": 0, "Time": "2019-12-17 21:58:39 -0500", "Prefix": "seq_3", "InitialPositionList": [ { "Label": "Pos0", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.435], "Focus": [16480.2], "XYStage": [ 69822.40000000001, 20100.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos1", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.425], "Focus": [16480.3], "XYStage": [ 69004, 20101 ] }, "GridColumnIndex": 0 }, { "Label": "Pos2", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.44], "Focus": [16480.1], "XYStage": [ 63119.5, 20690.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos3", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.405], "Focus": [16480.2], "XYStage": [ 62321, 20690.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos4", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.415], "Focus": [16480], "XYStage": [ 56604.100000000006, 20688.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos5", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.42000000000002], "Focus": [16480.1], "XYStage": [ 56034.200000000004, 20688.800000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos6", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.455], "Focus": [16480], "XYStage": [ 50889.3, 20496.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos7", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.43], "Focus": [16480.2], "XYStage": [ 50101.600000000006, 20496.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos8", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.67000000000002], "Focus": [16479.9], "XYStage": [ 44818.600000000006, 20496.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos9", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.67000000000002], "Focus": [16480], "XYStage": [ 43897.9, 20436.800000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos10", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.675], "Focus": [16479.9], "XYStage": [ 39670.600000000006, 20664.9 ] }, "GridColumnIndex": 0 }, { "Label": "Pos11", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.69], "Focus": [16479.9], "XYStage": [ 38734, 20664.600000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos12", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.695], "Focus": [16480.1], "XYStage": [ 34037.200000000004, 20664.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos13", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.685], "Focus": [16480.1], "XYStage": [ 33152, 20664.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos14", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.69], "Focus": [16480.1], "XYStage": [ 28122.800000000003, 20664.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos15", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.675], "Focus": [16480], "XYStage": [ 27422.9, 20664.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos16", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.64000000000001], "Focus": [16480], "XYStage": [ 23005.2, 20664.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos17", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.69], "Focus": [16479.9], "XYStage": [ 21820.600000000002, 20409.600000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos18", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.41], "Focus": [16480.1], "XYStage": [ 73697.6, -13222.800000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos19", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.455], "Focus": [16480.1], "XYStage": [ 72765.8, -13223 ] }, "GridColumnIndex": 0 }, { "Label": "Pos20", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.4], "Focus": [16480.2], "XYStage": [ 67445.5, -12303.800000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos21", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.415], "Focus": [16480.2], "XYStage": [ 66613.3, -12303.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos22", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.415], "Focus": [16480.1], "XYStage": [ 61819.4, -11936.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos23", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.405], "Focus": [16480.2], "XYStage": [ 61325.700000000004, -11937.1 ] }, "GridColumnIndex": 0 }, { "Label": "Pos24", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.46], "Focus": [16480.2], "XYStage": [ 55377, -10974.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos25", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.445], "Focus": [16480.1], "XYStage": [ 54827, -11214.800000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos26", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.435], "Focus": [16480.2], "XYStage": [ 49937.100000000006, -10919.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos27", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.425], "Focus": [16480.1], "XYStage": [ 48764, -10919.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos28", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.42000000000002], "Focus": [16480.2], "XYStage": [ 43978.4, -10920.400000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos29", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.435], "Focus": [16480.2], "XYStage": [ 43106.3, -10920.400000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos30", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.405], "Focus": [16480.3], "XYStage": [ 38212.4, -10920.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos31", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.445], "Focus": [16480.2], "XYStage": [ 37254.9, -10920.400000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos32", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.44], "Focus": [16480.3], "XYStage": [ 32472.800000000003, -11294.400000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos33", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [166.95000000000002], "Focus": [16480.2], "XYStage": [ 31420.7, -11294.400000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos34", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [166.95000000000002], "Focus": [16480.2], "XYStage": [ 26881.5, -11640 ] }, "GridColumnIndex": 0 }, { "Label": "Pos35", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [166.98], "Focus": [16480.2], "XYStage": [ 25876.100000000002, -11641.1 ] }, "GridColumnIndex": 0 } ], "z-step_um": 10, "GridRow": 0, "BitDepth": 16, "Depth": 2, "Channels": 6, "Source": "Micro-Manager", "ChColors": [ -16711681, -256, -65281, -1, -1, -16776961 ], "MetadataVersion": 10, "KeepShutterOpenChannels": true, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536, 65536 ], "CustomIntervals_ms": [], "Height": 1200, "MicroManagerVersion": "1.4.23 20180219", "GridColumn": 0, "UUID": "8c8f9d20-b128-4577-9f91-89e70f22d319", "PixelType": "GRAY16", "Width": 1200, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\Yan\\20191217-YC113-S5S6", "Frames": 1, "Comment": "", "UserName": "Nikon", "PixelSize_um": 1.1, "ROI": [ 0, 0, 1200, 1200 ], "Date": "2019-12-17", "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC", "P-DAPI-high" ], "KeepShutterOpenSlices": true, "IJType": 1, "ComputerName": "ZadorConfocal2", "Positions": 36, "PositionIndex": 3, "CameraTimeout": 20000, "TimeFirst": false }, "640 AO-Name": "AnalogIO", "Vincent-D3-Command": "Close" }, "FrameKey-0-4-4": { "COM4-StopBits": "1", "528 AO-MaxVolts": "5.0000", "XLIGHT Excitation Wheel-State": "2", "470 Shutter-Name": "AnalogIO", "Core-Shutter": "Vincent-D3", "555 AO-Demo": "No", "XLIGHT Dichroic-State": "2", "XYStage-StepSize-Y": "0.1000", "ZPositionUm": 167.405, "XYStage-StepSize-X": "0.1000", "PRIME-FirmwareVersion": "20.28", "PRIME-ClearCycles": "2", "520-Name": "DA Shutter", "405 Shutter-Name": "AnalogIO", "PIEZO-StepSize": "0.0050", "PRIME-ClearMode": "Pre-Exposure", "470 AO-MinVolts": "0.0000", "405 AO-SupportsTriggering": "1", "CameraChannelIndex": 0, "XYStage-StartSpeed": "500.0000", "COM9-StopBits": "2", "405 AO-Description": "NI DAC", "COM4-DelayBetweenCharsMs": "0.0000", "445 AO-Block voltage": "false", "520 AO-TriggerInputLine": "/Dev1/PFI0", "555 AO-Description": "NI DAC", "520 AO-TriggerSampleRate": "100000", "COM4-Verbose": "1", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM4-Name": "COM4", "405 Shutter-Volts": "0.0000", "Vincent-D3-Port": "COM1", "PRIME-PP 4 MIN ADU AFFECTED": "200", "470 Shutter-MinVolts": "0.0000", "PRIME-PP 3 ENABLED": "Yes", "470 AO-Description": "NI DAC", "PRIME-Description": "PVCAM API device adapter", "520 Shutter-MaxVolts": "5.0000", "520 Shutter-Volts": "0.0000", "528 AO-Sequenceable": "1", "PRIME-Binning": "1x1", "555 Shutter-MaxVolts": "5.0000", "528 Shutter-TriggerSequenceLength": "1000", "405 Shutter-Block voltage": "false", "XLIGHT Spin motor-State": "1", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "Vincent-D3-Time to open (ms)": "35.0000", "Vincent-D3-Time to close (ms)": "35.0000", "445 AO-TriggerSampleRate": "100000", "640 Shutter-IOChannel": "Dev1/ao14", "555 AO-Sequenceable": "1", "520 AO-Name": "AnalogIO", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "470 AO-Block voltage": "false", "COM1-Verbose": "1", "COM9-AnswerTimeout": "10000.0000", "528 Shutter-Name": "AnalogIO", "PRIME-Trigger Timeout (secs)": "10", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "NextFrame": 0, "470 AO-TriggerSequenceLength": "1000", "PRIME-TransposeXY": "0", "445 AO-TriggerSequenceLength": "1000", "COM1-Name": "COM1", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "COM9-DataBits": "8", "COM1-AnswerTimeout": "500.0000", "Camera": "", "TE2000-Version": "V2.50-V2.50-V2.10", "528 Shutter-Volts": "0.0000", "XLIGHT Hub-Name": "XLIGHT Hub", "PIEZO-Description": "Ludl stage driver adapter", "PositionName": "Pos3", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSampleRate": "100000", "PRIME-FanSpeedSetpoint": "High", "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "405 AO-TriggerSampleRate": "100000", "470 Shutter-Block voltage": "false", "470-State": "0", "COM3-Parity": "None", "405 AO-Volts": "0.0000", "PRIME-TransposeMirrorY": "0", "PRIME-ChipName": "GS144BSI", "470 AO-Volts": "0.0000", "PRIME-TransposeMirrorX": "0", "445 Shutter-SupportsTriggering": "0", "470 Shutter-SupportsTriggering": "0", "XLIGHT Hub-Description": "XLIGHT hub", "PRIME-PreampOffLimit": "10000", "470 AO-Sequenceable": "1", "528 Shutter-MaxVolts": "5.0000", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "Core-XYStage": "XYStage", "470 AO-TriggerSampleRate": "100000", "PRIME-Timing-PreTriggerDelayNs": "24672000.0000", "528-DA Device": "528 Shutter", "Focus-Description": "Nikon TE2000 focus adapter", "FilterBlock-State": "5", "520 AO-Demo": "No", "COM3-DelayBetweenCharsMs": "0.0000", "470 Shutter-Volts": "0.0000", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "PRIME-PP 2 THRESHOLD": "125", "PRIME-PP 4 THRESHOLD": "75", "XYStage-Acceleration": "4.0000", "470 Shutter-Demo": "No", "PRIME-Name": "PMPCIECam00", "640-DA Device": "640 Shutter", "405 AO-Block voltage": "false", "ElapsedTime-ms": 160971, "PRIME-Offset": "100", "445 AO-IOChannel": "Dev1/ao1", "640 AO-MaxVolts": "5.0000", "PositionIndex": 3, "PRIME-PP 1 ENABLED": "Yes", "COM1-BaudRate": "9600", "640-State": "0", "COM3-Verbose": "1", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "LudlController-TransmissionDelay": "0", "PRIME-FullWellCapacity": "62000", "PRIME-SMARTStreamingEnabled": "No", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "640 Shutter-Description": "NI DAC", "528-Description": "DA device that is used as a shutter", "COM4-Description": "Serial port driver (boost:asio)", "405 Shutter-Description": "NI DAC", "TE2000-Name": "TE2000", "528 Shutter-Description": "NI DAC", "COM9-Description": "Serial port driver (boost:asio)", "LudlController-Name": "LudlController", "445 Shutter-TriggerSequenceLength": "1000", "PRIME-SerialNumber": "A18B203008", "445 AO-Volts": "0.0000", "PRIME-X-dimension": "1200", "PRIME-ShutterCloseDelay": "0", "XLIGHT Disk slider-State": "2", "Position": "Default", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 AO-IOChannel": "Dev1/ao5", "520 AO-IOChannel": "Dev1/ao4", "528 AO-TriggerSequenceLength": "1000", "405 Shutter-MaxVolts": "5.0000", "470-DA Device": "470 Shutter", "PRIME-FTCapable": "No", "Core-ChannelGroup": "Channels", "555 AO-SupportsTriggering": "0", "PRIME-Y-dimension": "1200", "Frame": 0, "555 Shutter-Sequenceable": "1", "PRIME-Timing-ExposureTimeNs": "9992000.0000", "Core-ImageProcessor": "", "528-State": "0", "520 AO-Block voltage": "false", "XLIGHT Excitation Wheel-Label": "Excitation-2", "640 Shutter-Demo": "No", "COM3-StopBits": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "520 Shutter-IOChannel": "Dev1/ao11", "Width": 1200, "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-SupportsTriggering": "0", "640 AO-TriggerSampleRate": "100000", "520-Description": "DA device that is used as a shutter", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "405 Shutter-Sequenceable": "1", "405 Shutter-SupportsTriggering": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-TriggerSampleRate": "100000", "640 Shutter-MaxVolts": "5.0000", "555 AO-MaxVolts": "5.0000", "520 AO-TriggerSequenceLength": "1000", "COM3-BaudRate": "9600", "COM9-BaudRate": "9600", "Vincent-D3-Channel #": "Ch. #1", "PRIME-PMode": "Normal", "OpticalPath-Label": "Left(100)", "BitDepth": 16, "555 AO-TriggerSequenceLength": "1000", "445 Shutter-Description": "NI DAC", "405 AO-Sequenceable": "1", "PRIME-AcquisitionMethod": "Callbacks", "555 AO-Name": "AnalogIO", "PRIME-ShutterMode": "Pre-Exposure", "COM3-Handshaking": "Off", "445 AO-SupportsTriggering": "0", "ROI": "0-0-1200-1200", "COM9-Verbose": "1", "PRIME-Color": "OFF", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "PRIME-ShutterOpenDelay": "0", "COM1-Parity": "None", "Slice": 4, "SlicePosition": -10, "COM9-DelayBetweenCharsMs": "0.0000", "555-Name": "DA Shutter", "PIEZO-LudlSingleAxisName": "B", "XLIGHT Emission Wheel-Label": "Empty", "640 Shutter-Name": "AnalogIO", "640 AO-Sequenceable": "1", "520 Shutter-Demo": "No", "528 Shutter-Demo": "No", "470-Name": "DA Shutter", "520-DA Device": "520 Shutter", "XYStage-Description": "Ludl XY stage driver adapter", "445 Shutter-MinVolts": "0.0000", "528 AO-TriggerSampleRate": "100000", "405 AO-MinVolts": "0.0000", "405 Shutter-TriggerSequenceLength": "1000", "Height": 1200, "555 AO-Block voltage": "false", "640 Shutter-Volts": "0.0000", "PixelType": "GRAY16", "445 Shutter-Block voltage": "false", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "520 Shutter-TriggerSequenceLength": "1000", "COM9-Name": "COM9", "Core-SLM": "", "555 AO-Volts": "0.0000", "555 Shutter-Block voltage": "false", "XYStage-Speed": "1800.0000", "520 AO-MaxVolts": "5.0000", "528 Shutter-SupportsTriggering": "0", "COM1-Description": "Serial port driver (boost:asio)", "PRIME-MetadataEnabled": "No", "520 Shutter-MinVolts": "0.0000", "470 Shutter-Sequenceable": "1", "PRIME-PreampDelay": "0", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-MinVolts": "0.0000", "ChannelIndex": 4, "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Core-AutoShutter": "1", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "Exposure-ms": 20, "PRIME-PP 2 ENABLED": "Yes", "640 Shutter-TriggerSampleRate": "100000", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "470 Shutter-IOChannel": "Dev1/ao10", "405 AO-Demo": "No", "PixelSizeUm": 1.1, "470 AO-Demo": "No", "470 Shutter-MaxVolts": "5.0000", "Vincent-D3-Name": "Vincent-D3", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FileName": "img_000000000_P-DIC_004.tif", "LudlController-Reset": "Operate", "PRIME-Exposure": "20.00", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "445-Name": "DA Shutter", "555 Shutter-Name": "AnalogIO", "520 Shutter-TriggerSampleRate": "100000", "445 Shutter-IOChannel": "Dev1/ao9", "PRIME-TransposeCorrection": "0", "XYStage-StepSize": "0.1000", "445-Description": "DA device that is used as a shutter", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 AO-IOChannel": "Dev1/ao7", "520 Shutter-Name": "AnalogIO", "COM3-DataBits": "8", "640 AO-Volts": "0.0000", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "555 Shutter-IOChannel": "Dev1/ao13", "WaitInterval": null, "520 Shutter-Sequenceable": "1", "YPositionUm": 20690.300000000003, "445-State": "0", "445-DA Device": "445 Shutter", "PRIME-CircularBufferAutoSize": "ON", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "555 AO-TriggerSampleRate": "100000", "445 Shutter-Volts": "0.0000", "XLIGHT Hub-Port": "COM3", "Core-AutoFocus": "", "COM4-Handshaking": "Off", "528 Shutter-MinVolts": "0.0000", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "445 Shutter-Name": "AnalogIO", "528 Shutter-Sequenceable": "1", "XYStage-TransposeMirrorX": "0", "XYStage-TransposeMirrorY": "0", "PRIME-ReadoutRate": "100MHz 16bit", "XYStage-Name": "Stage", "640 AO-SupportsTriggering": "0", "640-Name": "DA Shutter", "640 Shutter-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "640 AO-Block voltage": "false", "445 Shutter-Sequenceable": "1", "470 AO-MaxVolts": "5.0000", "COM4-BaudRate": "9600", "470-Description": "DA device that is used as a shutter", "520 Shutter-Description": "NI DAC", "PRIME-CCDTemperature": "-14.9600", "Time": "2019-12-17 21:59:08 -0500", "640 AO-TriggerSequenceLength": "1000", "470 AO-Name": "AnalogIO", "XLIGHT Disk slider-Label": "Disk Pos 40um", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "Binning": "1x1", "TE2000-ModelType": "1", "COM3-AnswerTimeout": "500.0000", "555 Shutter-SupportsTriggering": "0", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "TE2000-Port": "COM4", "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Description": "NI DAC", "COM4-AnswerTimeout": "500.0000", "COM1-DelayBetweenCharsMs": "0.0000", "FilterBlock-Label": "Confocal", "445 AO-Name": "AnalogIO", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-Timing-ReadoutTimeNs": "24672000.0000", "528 AO-Demo": "No", "520 AO-Sequenceable": "1", "555-Description": "DA device that is used as a shutter", "405-DA Device": "405 Shutter", "405 AO-TriggerSequenceLength": "1000", "640 AO-MinVolts": "0.0000", "640 Shutter-Block voltage": "false", "FrameIndex": 0, "PRIME-Port": "CMOS", "555 AO-MinVolts": "0.0000", "COM3-Description": "Serial port driver (boost:asio)", "528 AO-Description": "NI DAC", "555 AO-IOChannel": "Dev1/ao6", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "555 AO-TriggerInputLine": "/Dev1/PFI0", "PRIME-ActualInterval-ms": "374.3700", "LudlController-Port": "COM9", "445 Shutter-Demo": "No", "PRIME-Trigger-Expose Out-Mux": "1", "SliceIndex": 4, "PRIME-CCDTemperatureSetPoint": "-15.0000", "405 Shutter-MinVolts": "0.0000", "PRIME-ExposeOutMode": "First Row", "AxisPositions": null, "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Volts": "0.0000", "528-Name": "DA Shutter", "555-State": "0", "XLIGHT Dichroic-Label": "Di03-R635-t1", "445 AO-Sequenceable": "1", "405-Name": "DA Shutter", "405 AO-IOChannel": "Dev1/ao0", "Core-Camera": "PRIME", "Focus-Name": "Focus", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "405 Shutter-IOChannel": "Dev1/ao8", "640 AO-Demo": "No", "640 Shutter-SupportsTriggering": "0", "445 AO-Demo": "No", "555-DA Device": "555 Shutter", "470 Shutter-Description": "NI DAC", "528 AO-Volts": "0.0000", "COM1-DataBits": "8", "Vincent-D3-Address": "x", "520 AO-SupportsTriggering": "0", "405-Description": "DA device that is used as a shutter", "640 Shutter-MinVolts": "0.0000", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "405 AO-Name": "AnalogIO", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "640 AO-TriggerInputLine": "/Dev1/PFI0", "405 AO-TriggerInputLine": "/Dev1/PFI0", "640-Description": "DA device that is used as a shutter", "445 Shutter-TriggerSampleRate": "100000", "470 Shutter-TriggerSequenceLength": "1000", "COM1-StopBits": "1", "520 Shutter-SupportsTriggering": "0", "405-State": "0", "Core-Initialize": "1", "XLIGHT Emission Wheel-State": "7", "520 AO-Description": "NI DAC", "528 AO-TriggerInputLine": "/Dev1/PFI0", "445 Shutter-MaxVolts": "5.0000", "COM9-Handshaking": "Off", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "XLIGHT Spin motor-Label": "On (spinning)", "PRIME-PP 5 ENABLED": "No", "UUID": "9184ad72-ef3a-44fb-808e-616b90ae33c0", "PRIME-TriggerMode": "Internal Trigger", "COM1-Handshaking": "Off", "PRIME-Timing-PostTriggerDelayNs": "49344000.0000", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "Focus-Position": "16479.8000", "528 Shutter-Block voltage": "false", "Channel": "P-DIC", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "555 Shutter-Demo": "No", "405 Shutter-Demo": "No", "528 AO-Name": "AnalogIO", "520-State": "0", "Core-Galvo": "", "405 Shutter-TriggerSampleRate": "100000", "Focus-FocusStepSize_(nm)": "100", "PRIME-PixelType": "16bit", "COM4-DataBits": "8", "470 AO-IOChannel": "Dev1/ao2", "520 AO-MinVolts": "0.0000", "COM9-Parity": "None", "PRIME-CircularBufferEnabled": "ON", "PRIME-PP 6 Reset": "No", "640 AO-Description": "NI DAC", "Source": "PRIME", "445 AO-MinVolts": "0.0000", "PIEZO-Name": "Stage", "445 AO-Description": "NI DAC", "PRIME-CircularBufferFrameCount": "46", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "520 AO-Volts": "0.0000", "528 Shutter-TriggerSampleRate": "100000", "XPositionUm": 62321, "OpticalPath-State": "4", "PRIME-PP 3 THRESHOLD": "80", "PRIME-PVCAM Version": "3.7.1", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Gain": "1-HDR", "640 Shutter-TriggerSequenceLength": "1000", "528 AO-SupportsTriggering": "0", "445 AO-TriggerInputLine": "/Dev1/PFI0", "OpticalPath-Name": "OpticalPath", "PRIME-PP 4 ENABLED": "Yes", "PRIME-PVCAM Adapter Version": "1.3.29", "COM3-Name": "COM3", "405 AO-MaxVolts": "5.0000", "520 Shutter-Block voltage": "false", "PRIME-Timing-ClearingTimeNs": "49344000.0000", "Core-Focus": "PIEZO", "528 AO-Block voltage": "false", "TE2000-Description": "Nikon TE2000 hub", "528 AO-MinVolts": "0.0000", "COM4-Parity": "None", "Summary": { "Slices": 11, "ChContrastMin": [ 0, 0, 0, 0, 0, 0 ], "PixelAspect": 1, "SlicesFirst": true, "Interval_ms": 0, "Time": "2019-12-17 21:58:39 -0500", "Prefix": "seq_3", "InitialPositionList": [ { "Label": "Pos0", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.435], "Focus": [16480.2], "XYStage": [ 69822.40000000001, 20100.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos1", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.425], "Focus": [16480.3], "XYStage": [ 69004, 20101 ] }, "GridColumnIndex": 0 }, { "Label": "Pos2", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.44], "Focus": [16480.1], "XYStage": [ 63119.5, 20690.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos3", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.405], "Focus": [16480.2], "XYStage": [ 62321, 20690.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos4", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.415], "Focus": [16480], "XYStage": [ 56604.100000000006, 20688.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos5", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.42000000000002], "Focus": [16480.1], "XYStage": [ 56034.200000000004, 20688.800000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos6", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.455], "Focus": [16480], "XYStage": [ 50889.3, 20496.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos7", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.43], "Focus": [16480.2], "XYStage": [ 50101.600000000006, 20496.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos8", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.67000000000002], "Focus": [16479.9], "XYStage": [ 44818.600000000006, 20496.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos9", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.67000000000002], "Focus": [16480], "XYStage": [ 43897.9, 20436.800000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos10", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.675], "Focus": [16479.9], "XYStage": [ 39670.600000000006, 20664.9 ] }, "GridColumnIndex": 0 }, { "Label": "Pos11", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.69], "Focus": [16479.9], "XYStage": [ 38734, 20664.600000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos12", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.695], "Focus": [16480.1], "XYStage": [ 34037.200000000004, 20664.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos13", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.685], "Focus": [16480.1], "XYStage": [ 33152, 20664.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos14", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.69], "Focus": [16480.1], "XYStage": [ 28122.800000000003, 20664.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos15", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.675], "Focus": [16480], "XYStage": [ 27422.9, 20664.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos16", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.64000000000001], "Focus": [16480], "XYStage": [ 23005.2, 20664.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos17", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.69], "Focus": [16479.9], "XYStage": [ 21820.600000000002, 20409.600000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos18", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.41], "Focus": [16480.1], "XYStage": [ 73697.6, -13222.800000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos19", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.455], "Focus": [16480.1], "XYStage": [ 72765.8, -13223 ] }, "GridColumnIndex": 0 }, { "Label": "Pos20", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.4], "Focus": [16480.2], "XYStage": [ 67445.5, -12303.800000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos21", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.415], "Focus": [16480.2], "XYStage": [ 66613.3, -12303.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos22", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.415], "Focus": [16480.1], "XYStage": [ 61819.4, -11936.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos23", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.405], "Focus": [16480.2], "XYStage": [ 61325.700000000004, -11937.1 ] }, "GridColumnIndex": 0 }, { "Label": "Pos24", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.46], "Focus": [16480.2], "XYStage": [ 55377, -10974.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos25", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.445], "Focus": [16480.1], "XYStage": [ 54827, -11214.800000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos26", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.435], "Focus": [16480.2], "XYStage": [ 49937.100000000006, -10919.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos27", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.425], "Focus": [16480.1], "XYStage": [ 48764, -10919.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos28", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.42000000000002], "Focus": [16480.2], "XYStage": [ 43978.4, -10920.400000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos29", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.435], "Focus": [16480.2], "XYStage": [ 43106.3, -10920.400000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos30", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.405], "Focus": [16480.3], "XYStage": [ 38212.4, -10920.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos31", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.445], "Focus": [16480.2], "XYStage": [ 37254.9, -10920.400000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos32", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.44], "Focus": [16480.3], "XYStage": [ 32472.800000000003, -11294.400000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos33", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [166.95000000000002], "Focus": [16480.2], "XYStage": [ 31420.7, -11294.400000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos34", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [166.95000000000002], "Focus": [16480.2], "XYStage": [ 26881.5, -11640 ] }, "GridColumnIndex": 0 }, { "Label": "Pos35", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [166.98], "Focus": [16480.2], "XYStage": [ 25876.100000000002, -11641.1 ] }, "GridColumnIndex": 0 } ], "z-step_um": 10, "GridRow": 0, "BitDepth": 16, "Depth": 2, "Channels": 6, "Source": "Micro-Manager", "ChColors": [ -16711681, -256, -65281, -1, -1, -16776961 ], "MetadataVersion": 10, "KeepShutterOpenChannels": true, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536, 65536 ], "CustomIntervals_ms": [], "Height": 1200, "MicroManagerVersion": "1.4.23 20180219", "GridColumn": 0, "UUID": "8c8f9d20-b128-4577-9f91-89e70f22d319", "PixelType": "GRAY16", "Width": 1200, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\Yan\\20191217-YC113-S5S6", "Frames": 1, "Comment": "", "UserName": "Nikon", "PixelSize_um": 1.1, "ROI": [ 0, 0, 1200, 1200 ], "Date": "2019-12-17", "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC", "P-DAPI-high" ], "KeepShutterOpenSlices": true, "IJType": 1, "ComputerName": "ZadorConfocal2", "Positions": 36, "PositionIndex": 3, "CameraTimeout": 20000, "TimeFirst": false }, "640 AO-Name": "AnalogIO", "Vincent-D3-Command": "Close" }, "FrameKey-0-4-5": { "COM4-StopBits": "1", "528 AO-MaxVolts": "5.0000", "XLIGHT Excitation Wheel-State": "2", "470 Shutter-Name": "AnalogIO", "Core-Shutter": "Vincent-D3", "555 AO-Demo": "No", "XLIGHT Dichroic-State": "2", "XYStage-StepSize-Y": "0.1000", "ZPositionUm": 177.405, "XYStage-StepSize-X": "0.1000", "PRIME-FirmwareVersion": "20.28", "PRIME-ClearCycles": "2", "520-Name": "DA Shutter", "405 Shutter-Name": "AnalogIO", "PIEZO-StepSize": "0.0050", "PRIME-ClearMode": "Pre-Exposure", "470 AO-MinVolts": "0.0000", "405 AO-SupportsTriggering": "1", "CameraChannelIndex": 0, "XYStage-StartSpeed": "500.0000", "COM9-StopBits": "2", "405 AO-Description": "NI DAC", "COM4-DelayBetweenCharsMs": "0.0000", "445 AO-Block voltage": "false", "520 AO-TriggerInputLine": "/Dev1/PFI0", "555 AO-Description": "NI DAC", "520 AO-TriggerSampleRate": "100000", "COM4-Verbose": "1", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM4-Name": "COM4", "405 Shutter-Volts": "0.0000", "Vincent-D3-Port": "COM1", "PRIME-PP 4 MIN ADU AFFECTED": "200", "470 Shutter-MinVolts": "0.0000", "PRIME-PP 3 ENABLED": "Yes", "470 AO-Description": "NI DAC", "PRIME-Description": "PVCAM API device adapter", "520 Shutter-MaxVolts": "5.0000", "520 Shutter-Volts": "0.0000", "528 AO-Sequenceable": "1", "PRIME-Binning": "1x1", "555 Shutter-MaxVolts": "5.0000", "528 Shutter-TriggerSequenceLength": "1000", "405 Shutter-Block voltage": "false", "XLIGHT Spin motor-State": "1", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "Vincent-D3-Time to open (ms)": "35.0000", "Vincent-D3-Time to close (ms)": "35.0000", "445 AO-TriggerSampleRate": "100000", "640 Shutter-IOChannel": "Dev1/ao14", "555 AO-Sequenceable": "1", "520 AO-Name": "AnalogIO", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "470 AO-Block voltage": "false", "COM1-Verbose": "1", "COM9-AnswerTimeout": "10000.0000", "528 Shutter-Name": "AnalogIO", "PRIME-Trigger Timeout (secs)": "10", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "NextFrame": 0, "470 AO-TriggerSequenceLength": "1000", "PRIME-TransposeXY": "0", "445 AO-TriggerSequenceLength": "1000", "COM1-Name": "COM1", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "COM9-DataBits": "8", "COM1-AnswerTimeout": "500.0000", "Camera": "", "TE2000-Version": "V2.50-V2.50-V2.10", "528 Shutter-Volts": "0.0000", "XLIGHT Hub-Name": "XLIGHT Hub", "PIEZO-Description": "Ludl stage driver adapter", "PositionName": "Pos3", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSampleRate": "100000", "PRIME-FanSpeedSetpoint": "High", "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "405 AO-TriggerSampleRate": "100000", "470 Shutter-Block voltage": "false", "470-State": "0", "COM3-Parity": "None", "405 AO-Volts": "0.0000", "PRIME-TransposeMirrorY": "0", "PRIME-ChipName": "GS144BSI", "470 AO-Volts": "0.0000", "PRIME-TransposeMirrorX": "0", "445 Shutter-SupportsTriggering": "0", "470 Shutter-SupportsTriggering": "0", "XLIGHT Hub-Description": "XLIGHT hub", "PRIME-PreampOffLimit": "10000", "470 AO-Sequenceable": "1", "528 Shutter-MaxVolts": "5.0000", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "Core-XYStage": "XYStage", "470 AO-TriggerSampleRate": "100000", "PRIME-Timing-PreTriggerDelayNs": "24672000.0000", "528-DA Device": "528 Shutter", "Focus-Description": "Nikon TE2000 focus adapter", "FilterBlock-State": "5", "520 AO-Demo": "No", "COM3-DelayBetweenCharsMs": "0.0000", "470 Shutter-Volts": "0.0000", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "PRIME-PP 2 THRESHOLD": "125", "PRIME-PP 4 THRESHOLD": "75", "XYStage-Acceleration": "4.0000", "470 Shutter-Demo": "No", "PRIME-Name": "PMPCIECam00", "640-DA Device": "640 Shutter", "405 AO-Block voltage": "false", "ElapsedTime-ms": 161241, "PRIME-Offset": "100", "445 AO-IOChannel": "Dev1/ao1", "640 AO-MaxVolts": "5.0000", "PositionIndex": 3, "PRIME-PP 1 ENABLED": "Yes", "COM1-BaudRate": "9600", "640-State": "0", "COM3-Verbose": "1", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "LudlController-TransmissionDelay": "0", "PRIME-FullWellCapacity": "62000", "PRIME-SMARTStreamingEnabled": "No", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "640 Shutter-Description": "NI DAC", "528-Description": "DA device that is used as a shutter", "COM4-Description": "Serial port driver (boost:asio)", "405 Shutter-Description": "NI DAC", "TE2000-Name": "TE2000", "528 Shutter-Description": "NI DAC", "COM9-Description": "Serial port driver (boost:asio)", "LudlController-Name": "LudlController", "445 Shutter-TriggerSequenceLength": "1000", "PRIME-SerialNumber": "A18B203008", "445 AO-Volts": "0.0000", "PRIME-X-dimension": "1200", "PRIME-ShutterCloseDelay": "0", "XLIGHT Disk slider-State": "2", "Position": "Default", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 AO-IOChannel": "Dev1/ao5", "520 AO-IOChannel": "Dev1/ao4", "528 AO-TriggerSequenceLength": "1000", "405 Shutter-MaxVolts": "5.0000", "470-DA Device": "470 Shutter", "PRIME-FTCapable": "No", "Core-ChannelGroup": "Channels", "555 AO-SupportsTriggering": "0", "PRIME-Y-dimension": "1200", "Frame": 0, "555 Shutter-Sequenceable": "1", "PRIME-Timing-ExposureTimeNs": "9992000.0000", "Core-ImageProcessor": "", "528-State": "0", "520 AO-Block voltage": "false", "XLIGHT Excitation Wheel-Label": "Excitation-2", "640 Shutter-Demo": "No", "COM3-StopBits": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "520 Shutter-IOChannel": "Dev1/ao11", "Width": 1200, "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-SupportsTriggering": "0", "640 AO-TriggerSampleRate": "100000", "520-Description": "DA device that is used as a shutter", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "405 Shutter-Sequenceable": "1", "405 Shutter-SupportsTriggering": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-TriggerSampleRate": "100000", "640 Shutter-MaxVolts": "5.0000", "555 AO-MaxVolts": "5.0000", "520 AO-TriggerSequenceLength": "1000", "COM3-BaudRate": "9600", "COM9-BaudRate": "9600", "Vincent-D3-Channel #": "Ch. #1", "PRIME-PMode": "Normal", "OpticalPath-Label": "Left(100)", "BitDepth": 16, "555 AO-TriggerSequenceLength": "1000", "445 Shutter-Description": "NI DAC", "405 AO-Sequenceable": "1", "PRIME-AcquisitionMethod": "Callbacks", "555 AO-Name": "AnalogIO", "PRIME-ShutterMode": "Pre-Exposure", "COM3-Handshaking": "Off", "445 AO-SupportsTriggering": "0", "ROI": "0-0-1200-1200", "COM9-Verbose": "1", "PRIME-Color": "OFF", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "PRIME-ShutterOpenDelay": "0", "COM1-Parity": "None", "Slice": 5, "SlicePosition": 0, "COM9-DelayBetweenCharsMs": "0.0000", "555-Name": "DA Shutter", "PIEZO-LudlSingleAxisName": "B", "XLIGHT Emission Wheel-Label": "Empty", "640 Shutter-Name": "AnalogIO", "640 AO-Sequenceable": "1", "520 Shutter-Demo": "No", "528 Shutter-Demo": "No", "470-Name": "DA Shutter", "520-DA Device": "520 Shutter", "XYStage-Description": "Ludl XY stage driver adapter", "445 Shutter-MinVolts": "0.0000", "528 AO-TriggerSampleRate": "100000", "405 AO-MinVolts": "0.0000", "405 Shutter-TriggerSequenceLength": "1000", "Height": 1200, "555 AO-Block voltage": "false", "640 Shutter-Volts": "0.0000", "PixelType": "GRAY16", "445 Shutter-Block voltage": "false", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "520 Shutter-TriggerSequenceLength": "1000", "COM9-Name": "COM9", "Core-SLM": "", "555 AO-Volts": "0.0000", "555 Shutter-Block voltage": "false", "XYStage-Speed": "1800.0000", "520 AO-MaxVolts": "5.0000", "528 Shutter-SupportsTriggering": "0", "COM1-Description": "Serial port driver (boost:asio)", "PRIME-MetadataEnabled": "No", "520 Shutter-MinVolts": "0.0000", "470 Shutter-Sequenceable": "1", "PRIME-PreampDelay": "0", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-MinVolts": "0.0000", "ChannelIndex": 4, "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Core-AutoShutter": "1", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "Exposure-ms": 20, "PRIME-PP 2 ENABLED": "Yes", "640 Shutter-TriggerSampleRate": "100000", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "470 Shutter-IOChannel": "Dev1/ao10", "405 AO-Demo": "No", "PixelSizeUm": 1.1, "470 AO-Demo": "No", "470 Shutter-MaxVolts": "5.0000", "Vincent-D3-Name": "Vincent-D3", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FileName": "img_000000000_P-DIC_005.tif", "LudlController-Reset": "Operate", "PRIME-Exposure": "20.00", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "445-Name": "DA Shutter", "555 Shutter-Name": "AnalogIO", "520 Shutter-TriggerSampleRate": "100000", "445 Shutter-IOChannel": "Dev1/ao9", "PRIME-TransposeCorrection": "0", "XYStage-StepSize": "0.1000", "445-Description": "DA device that is used as a shutter", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 AO-IOChannel": "Dev1/ao7", "520 Shutter-Name": "AnalogIO", "COM3-DataBits": "8", "640 AO-Volts": "0.0000", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "555 Shutter-IOChannel": "Dev1/ao13", "WaitInterval": null, "520 Shutter-Sequenceable": "1", "YPositionUm": 20690.300000000003, "445-State": "0", "445-DA Device": "445 Shutter", "PRIME-CircularBufferAutoSize": "ON", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "555 AO-TriggerSampleRate": "100000", "445 Shutter-Volts": "0.0000", "XLIGHT Hub-Port": "COM3", "Core-AutoFocus": "", "COM4-Handshaking": "Off", "528 Shutter-MinVolts": "0.0000", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "445 Shutter-Name": "AnalogIO", "528 Shutter-Sequenceable": "1", "XYStage-TransposeMirrorX": "0", "XYStage-TransposeMirrorY": "0", "PRIME-ReadoutRate": "100MHz 16bit", "XYStage-Name": "Stage", "640 AO-SupportsTriggering": "0", "640-Name": "DA Shutter", "640 Shutter-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "640 AO-Block voltage": "false", "445 Shutter-Sequenceable": "1", "470 AO-MaxVolts": "5.0000", "COM4-BaudRate": "9600", "470-Description": "DA device that is used as a shutter", "520 Shutter-Description": "NI DAC", "PRIME-CCDTemperature": "-14.9600", "Time": "2019-12-17 21:59:08 -0500", "640 AO-TriggerSequenceLength": "1000", "470 AO-Name": "AnalogIO", "XLIGHT Disk slider-Label": "Disk Pos 40um", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "Binning": "1x1", "TE2000-ModelType": "1", "COM3-AnswerTimeout": "500.0000", "555 Shutter-SupportsTriggering": "0", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "TE2000-Port": "COM4", "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Description": "NI DAC", "COM4-AnswerTimeout": "500.0000", "COM1-DelayBetweenCharsMs": "0.0000", "FilterBlock-Label": "Confocal", "445 AO-Name": "AnalogIO", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-Timing-ReadoutTimeNs": "24672000.0000", "528 AO-Demo": "No", "520 AO-Sequenceable": "1", "555-Description": "DA device that is used as a shutter", "405-DA Device": "405 Shutter", "405 AO-TriggerSequenceLength": "1000", "640 AO-MinVolts": "0.0000", "640 Shutter-Block voltage": "false", "FrameIndex": 0, "PRIME-Port": "CMOS", "555 AO-MinVolts": "0.0000", "COM3-Description": "Serial port driver (boost:asio)", "528 AO-Description": "NI DAC", "555 AO-IOChannel": "Dev1/ao6", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "555 AO-TriggerInputLine": "/Dev1/PFI0", "PRIME-ActualInterval-ms": "374.3700", "LudlController-Port": "COM9", "445 Shutter-Demo": "No", "PRIME-Trigger-Expose Out-Mux": "1", "SliceIndex": 5, "PRIME-CCDTemperatureSetPoint": "-15.0000", "405 Shutter-MinVolts": "0.0000", "PRIME-ExposeOutMode": "First Row", "AxisPositions": null, "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Volts": "0.0000", "528-Name": "DA Shutter", "555-State": "0", "XLIGHT Dichroic-Label": "Di03-R635-t1", "445 AO-Sequenceable": "1", "405-Name": "DA Shutter", "405 AO-IOChannel": "Dev1/ao0", "Core-Camera": "PRIME", "Focus-Name": "Focus", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "405 Shutter-IOChannel": "Dev1/ao8", "640 AO-Demo": "No", "640 Shutter-SupportsTriggering": "0", "445 AO-Demo": "No", "555-DA Device": "555 Shutter", "470 Shutter-Description": "NI DAC", "528 AO-Volts": "0.0000", "COM1-DataBits": "8", "Vincent-D3-Address": "x", "520 AO-SupportsTriggering": "0", "405-Description": "DA device that is used as a shutter", "640 Shutter-MinVolts": "0.0000", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "405 AO-Name": "AnalogIO", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "640 AO-TriggerInputLine": "/Dev1/PFI0", "405 AO-TriggerInputLine": "/Dev1/PFI0", "640-Description": "DA device that is used as a shutter", "445 Shutter-TriggerSampleRate": "100000", "470 Shutter-TriggerSequenceLength": "1000", "COM1-StopBits": "1", "520 Shutter-SupportsTriggering": "0", "405-State": "0", "Core-Initialize": "1", "XLIGHT Emission Wheel-State": "7", "520 AO-Description": "NI DAC", "528 AO-TriggerInputLine": "/Dev1/PFI0", "445 Shutter-MaxVolts": "5.0000", "COM9-Handshaking": "Off", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "XLIGHT Spin motor-Label": "On (spinning)", "PRIME-PP 5 ENABLED": "No", "UUID": "b0374b9a-ae15-4cd0-bff0-bbf13f8a71e7", "PRIME-TriggerMode": "Internal Trigger", "COM1-Handshaking": "Off", "PRIME-Timing-PostTriggerDelayNs": "49344000.0000", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "Focus-Position": "16479.8000", "528 Shutter-Block voltage": "false", "Channel": "P-DIC", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "555 Shutter-Demo": "No", "405 Shutter-Demo": "No", "528 AO-Name": "AnalogIO", "520-State": "0", "Core-Galvo": "", "405 Shutter-TriggerSampleRate": "100000", "Focus-FocusStepSize_(nm)": "100", "PRIME-PixelType": "16bit", "COM4-DataBits": "8", "470 AO-IOChannel": "Dev1/ao2", "520 AO-MinVolts": "0.0000", "COM9-Parity": "None", "PRIME-CircularBufferEnabled": "ON", "PRIME-PP 6 Reset": "No", "640 AO-Description": "NI DAC", "Source": "PRIME", "445 AO-MinVolts": "0.0000", "PIEZO-Name": "Stage", "445 AO-Description": "NI DAC", "PRIME-CircularBufferFrameCount": "46", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "520 AO-Volts": "0.0000", "528 Shutter-TriggerSampleRate": "100000", "XPositionUm": 62321, "OpticalPath-State": "4", "PRIME-PP 3 THRESHOLD": "80", "PRIME-PVCAM Version": "3.7.1", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Gain": "1-HDR", "640 Shutter-TriggerSequenceLength": "1000", "528 AO-SupportsTriggering": "0", "445 AO-TriggerInputLine": "/Dev1/PFI0", "OpticalPath-Name": "OpticalPath", "PRIME-PP 4 ENABLED": "Yes", "PRIME-PVCAM Adapter Version": "1.3.29", "COM3-Name": "COM3", "405 AO-MaxVolts": "5.0000", "520 Shutter-Block voltage": "false", "PRIME-Timing-ClearingTimeNs": "49344000.0000", "Core-Focus": "PIEZO", "528 AO-Block voltage": "false", "TE2000-Description": "Nikon TE2000 hub", "528 AO-MinVolts": "0.0000", "COM4-Parity": "None", "Summary": { "Slices": 11, "ChContrastMin": [ 0, 0, 0, 0, 0, 0 ], "PixelAspect": 1, "SlicesFirst": true, "Interval_ms": 0, "Time": "2019-12-17 21:58:39 -0500", "Prefix": "seq_3", "InitialPositionList": [ { "Label": "Pos0", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.435], "Focus": [16480.2], "XYStage": [ 69822.40000000001, 20100.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos1", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.425], "Focus": [16480.3], "XYStage": [ 69004, 20101 ] }, "GridColumnIndex": 0 }, { "Label": "Pos2", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.44], "Focus": [16480.1], "XYStage": [ 63119.5, 20690.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos3", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.405], "Focus": [16480.2], "XYStage": [ 62321, 20690.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos4", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.415], "Focus": [16480], "XYStage": [ 56604.100000000006, 20688.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos5", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.42000000000002], "Focus": [16480.1], "XYStage": [ 56034.200000000004, 20688.800000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos6", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.455], "Focus": [16480], "XYStage": [ 50889.3, 20496.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos7", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.43], "Focus": [16480.2], "XYStage": [ 50101.600000000006, 20496.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos8", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.67000000000002], "Focus": [16479.9], "XYStage": [ 44818.600000000006, 20496.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos9", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.67000000000002], "Focus": [16480], "XYStage": [ 43897.9, 20436.800000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos10", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.675], "Focus": [16479.9], "XYStage": [ 39670.600000000006, 20664.9 ] }, "GridColumnIndex": 0 }, { "Label": "Pos11", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.69], "Focus": [16479.9], "XYStage": [ 38734, 20664.600000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos12", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.695], "Focus": [16480.1], "XYStage": [ 34037.200000000004, 20664.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos13", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.685], "Focus": [16480.1], "XYStage": [ 33152, 20664.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos14", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.69], "Focus": [16480.1], "XYStage": [ 28122.800000000003, 20664.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos15", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.675], "Focus": [16480], "XYStage": [ 27422.9, 20664.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos16", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.64000000000001], "Focus": [16480], "XYStage": [ 23005.2, 20664.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos17", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.69], "Focus": [16479.9], "XYStage": [ 21820.600000000002, 20409.600000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos18", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.41], "Focus": [16480.1], "XYStage": [ 73697.6, -13222.800000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos19", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.455], "Focus": [16480.1], "XYStage": [ 72765.8, -13223 ] }, "GridColumnIndex": 0 }, { "Label": "Pos20", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.4], "Focus": [16480.2], "XYStage": [ 67445.5, -12303.800000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos21", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.415], "Focus": [16480.2], "XYStage": [ 66613.3, -12303.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos22", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.415], "Focus": [16480.1], "XYStage": [ 61819.4, -11936.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos23", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.405], "Focus": [16480.2], "XYStage": [ 61325.700000000004, -11937.1 ] }, "GridColumnIndex": 0 }, { "Label": "Pos24", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.46], "Focus": [16480.2], "XYStage": [ 55377, -10974.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos25", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.445], "Focus": [16480.1], "XYStage": [ 54827, -11214.800000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos26", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.435], "Focus": [16480.2], "XYStage": [ 49937.100000000006, -10919.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos27", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.425], "Focus": [16480.1], "XYStage": [ 48764, -10919.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos28", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.42000000000002], "Focus": [16480.2], "XYStage": [ 43978.4, -10920.400000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos29", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.435], "Focus": [16480.2], "XYStage": [ 43106.3, -10920.400000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos30", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.405], "Focus": [16480.3], "XYStage": [ 38212.4, -10920.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos31", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.445], "Focus": [16480.2], "XYStage": [ 37254.9, -10920.400000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos32", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.44], "Focus": [16480.3], "XYStage": [ 32472.800000000003, -11294.400000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos33", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [166.95000000000002], "Focus": [16480.2], "XYStage": [ 31420.7, -11294.400000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos34", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [166.95000000000002], "Focus": [16480.2], "XYStage": [ 26881.5, -11640 ] }, "GridColumnIndex": 0 }, { "Label": "Pos35", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [166.98], "Focus": [16480.2], "XYStage": [ 25876.100000000002, -11641.1 ] }, "GridColumnIndex": 0 } ], "z-step_um": 10, "GridRow": 0, "BitDepth": 16, "Depth": 2, "Channels": 6, "Source": "Micro-Manager", "ChColors": [ -16711681, -256, -65281, -1, -1, -16776961 ], "MetadataVersion": 10, "KeepShutterOpenChannels": true, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536, 65536 ], "CustomIntervals_ms": [], "Height": 1200, "MicroManagerVersion": "1.4.23 20180219", "GridColumn": 0, "UUID": "8c8f9d20-b128-4577-9f91-89e70f22d319", "PixelType": "GRAY16", "Width": 1200, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\Yan\\20191217-YC113-S5S6", "Frames": 1, "Comment": "", "UserName": "Nikon", "PixelSize_um": 1.1, "ROI": [ 0, 0, 1200, 1200 ], "Date": "2019-12-17", "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC", "P-DAPI-high" ], "KeepShutterOpenSlices": true, "IJType": 1, "ComputerName": "ZadorConfocal2", "Positions": 36, "PositionIndex": 3, "CameraTimeout": 20000, "TimeFirst": false }, "640 AO-Name": "AnalogIO", "Vincent-D3-Command": "Close" }, "FrameKey-0-4-6": { "COM4-StopBits": "1", "528 AO-MaxVolts": "5.0000", "XLIGHT Excitation Wheel-State": "2", "470 Shutter-Name": "AnalogIO", "Core-Shutter": "Vincent-D3", "555 AO-Demo": "No", "XLIGHT Dichroic-State": "2", "XYStage-StepSize-Y": "0.1000", "ZPositionUm": 187.405, "XYStage-StepSize-X": "0.1000", "PRIME-FirmwareVersion": "20.28", "PRIME-ClearCycles": "2", "520-Name": "DA Shutter", "405 Shutter-Name": "AnalogIO", "PIEZO-StepSize": "0.0050", "PRIME-ClearMode": "Pre-Exposure", "470 AO-MinVolts": "0.0000", "405 AO-SupportsTriggering": "1", "CameraChannelIndex": 0, "XYStage-StartSpeed": "500.0000", "COM9-StopBits": "2", "405 AO-Description": "NI DAC", "COM4-DelayBetweenCharsMs": "0.0000", "445 AO-Block voltage": "false", "520 AO-TriggerInputLine": "/Dev1/PFI0", "555 AO-Description": "NI DAC", "520 AO-TriggerSampleRate": "100000", "COM4-Verbose": "1", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM4-Name": "COM4", "405 Shutter-Volts": "0.0000", "Vincent-D3-Port": "COM1", "PRIME-PP 4 MIN ADU AFFECTED": "200", "470 Shutter-MinVolts": "0.0000", "PRIME-PP 3 ENABLED": "Yes", "470 AO-Description": "NI DAC", "PRIME-Description": "PVCAM API device adapter", "520 Shutter-MaxVolts": "5.0000", "520 Shutter-Volts": "0.0000", "528 AO-Sequenceable": "1", "PRIME-Binning": "1x1", "555 Shutter-MaxVolts": "5.0000", "528 Shutter-TriggerSequenceLength": "1000", "405 Shutter-Block voltage": "false", "XLIGHT Spin motor-State": "1", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "Vincent-D3-Time to open (ms)": "35.0000", "Vincent-D3-Time to close (ms)": "35.0000", "445 AO-TriggerSampleRate": "100000", "640 Shutter-IOChannel": "Dev1/ao14", "555 AO-Sequenceable": "1", "520 AO-Name": "AnalogIO", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "470 AO-Block voltage": "false", "COM1-Verbose": "1", "COM9-AnswerTimeout": "10000.0000", "528 Shutter-Name": "AnalogIO", "PRIME-Trigger Timeout (secs)": "10", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "NextFrame": 0, "470 AO-TriggerSequenceLength": "1000", "PRIME-TransposeXY": "0", "445 AO-TriggerSequenceLength": "1000", "COM1-Name": "COM1", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "COM9-DataBits": "8", "COM1-AnswerTimeout": "500.0000", "Camera": "", "TE2000-Version": "V2.50-V2.50-V2.10", "528 Shutter-Volts": "0.0000", "XLIGHT Hub-Name": "XLIGHT Hub", "PIEZO-Description": "Ludl stage driver adapter", "PositionName": "Pos3", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSampleRate": "100000", "PRIME-FanSpeedSetpoint": "High", "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "405 AO-TriggerSampleRate": "100000", "470 Shutter-Block voltage": "false", "470-State": "0", "COM3-Parity": "None", "405 AO-Volts": "0.0000", "PRIME-TransposeMirrorY": "0", "PRIME-ChipName": "GS144BSI", "470 AO-Volts": "0.0000", "PRIME-TransposeMirrorX": "0", "445 Shutter-SupportsTriggering": "0", "470 Shutter-SupportsTriggering": "0", "XLIGHT Hub-Description": "XLIGHT hub", "PRIME-PreampOffLimit": "10000", "470 AO-Sequenceable": "1", "528 Shutter-MaxVolts": "5.0000", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "Core-XYStage": "XYStage", "470 AO-TriggerSampleRate": "100000", "PRIME-Timing-PreTriggerDelayNs": "24672000.0000", "528-DA Device": "528 Shutter", "Focus-Description": "Nikon TE2000 focus adapter", "FilterBlock-State": "5", "520 AO-Demo": "No", "COM3-DelayBetweenCharsMs": "0.0000", "470 Shutter-Volts": "0.0000", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "PRIME-PP 2 THRESHOLD": "125", "PRIME-PP 4 THRESHOLD": "75", "XYStage-Acceleration": "4.0000", "470 Shutter-Demo": "No", "PRIME-Name": "PMPCIECam00", "640-DA Device": "640 Shutter", "405 AO-Block voltage": "false", "ElapsedTime-ms": 161499, "PRIME-Offset": "100", "445 AO-IOChannel": "Dev1/ao1", "640 AO-MaxVolts": "5.0000", "PositionIndex": 3, "PRIME-PP 1 ENABLED": "Yes", "COM1-BaudRate": "9600", "640-State": "0", "COM3-Verbose": "1", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "LudlController-TransmissionDelay": "0", "PRIME-FullWellCapacity": "62000", "PRIME-SMARTStreamingEnabled": "No", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "640 Shutter-Description": "NI DAC", "528-Description": "DA device that is used as a shutter", "COM4-Description": "Serial port driver (boost:asio)", "405 Shutter-Description": "NI DAC", "TE2000-Name": "TE2000", "528 Shutter-Description": "NI DAC", "COM9-Description": "Serial port driver (boost:asio)", "LudlController-Name": "LudlController", "445 Shutter-TriggerSequenceLength": "1000", "PRIME-SerialNumber": "A18B203008", "445 AO-Volts": "0.0000", "PRIME-X-dimension": "1200", "PRIME-ShutterCloseDelay": "0", "XLIGHT Disk slider-State": "2", "Position": "Default", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 AO-IOChannel": "Dev1/ao5", "520 AO-IOChannel": "Dev1/ao4", "528 AO-TriggerSequenceLength": "1000", "405 Shutter-MaxVolts": "5.0000", "470-DA Device": "470 Shutter", "PRIME-FTCapable": "No", "Core-ChannelGroup": "Channels", "555 AO-SupportsTriggering": "0", "PRIME-Y-dimension": "1200", "Frame": 0, "555 Shutter-Sequenceable": "1", "PRIME-Timing-ExposureTimeNs": "9992000.0000", "Core-ImageProcessor": "", "528-State": "0", "520 AO-Block voltage": "false", "XLIGHT Excitation Wheel-Label": "Excitation-2", "640 Shutter-Demo": "No", "COM3-StopBits": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "520 Shutter-IOChannel": "Dev1/ao11", "Width": 1200, "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-SupportsTriggering": "0", "640 AO-TriggerSampleRate": "100000", "520-Description": "DA device that is used as a shutter", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "405 Shutter-Sequenceable": "1", "405 Shutter-SupportsTriggering": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-TriggerSampleRate": "100000", "640 Shutter-MaxVolts": "5.0000", "555 AO-MaxVolts": "5.0000", "520 AO-TriggerSequenceLength": "1000", "COM3-BaudRate": "9600", "COM9-BaudRate": "9600", "Vincent-D3-Channel #": "Ch. #1", "PRIME-PMode": "Normal", "OpticalPath-Label": "Left(100)", "BitDepth": 16, "555 AO-TriggerSequenceLength": "1000", "445 Shutter-Description": "NI DAC", "405 AO-Sequenceable": "1", "PRIME-AcquisitionMethod": "Callbacks", "555 AO-Name": "AnalogIO", "PRIME-ShutterMode": "Pre-Exposure", "COM3-Handshaking": "Off", "445 AO-SupportsTriggering": "0", "ROI": "0-0-1200-1200", "COM9-Verbose": "1", "PRIME-Color": "OFF", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "PRIME-ShutterOpenDelay": "0", "COM1-Parity": "None", "Slice": 6, "SlicePosition": 10, "COM9-DelayBetweenCharsMs": "0.0000", "555-Name": "DA Shutter", "PIEZO-LudlSingleAxisName": "B", "XLIGHT Emission Wheel-Label": "Empty", "640 Shutter-Name": "AnalogIO", "640 AO-Sequenceable": "1", "520 Shutter-Demo": "No", "528 Shutter-Demo": "No", "470-Name": "DA Shutter", "520-DA Device": "520 Shutter", "XYStage-Description": "Ludl XY stage driver adapter", "445 Shutter-MinVolts": "0.0000", "528 AO-TriggerSampleRate": "100000", "405 AO-MinVolts": "0.0000", "405 Shutter-TriggerSequenceLength": "1000", "Height": 1200, "555 AO-Block voltage": "false", "640 Shutter-Volts": "0.0000", "PixelType": "GRAY16", "445 Shutter-Block voltage": "false", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "520 Shutter-TriggerSequenceLength": "1000", "COM9-Name": "COM9", "Core-SLM": "", "555 AO-Volts": "0.0000", "555 Shutter-Block voltage": "false", "XYStage-Speed": "1800.0000", "520 AO-MaxVolts": "5.0000", "528 Shutter-SupportsTriggering": "0", "COM1-Description": "Serial port driver (boost:asio)", "PRIME-MetadataEnabled": "No", "520 Shutter-MinVolts": "0.0000", "470 Shutter-Sequenceable": "1", "PRIME-PreampDelay": "0", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-MinVolts": "0.0000", "ChannelIndex": 4, "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Core-AutoShutter": "1", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "Exposure-ms": 20, "PRIME-PP 2 ENABLED": "Yes", "640 Shutter-TriggerSampleRate": "100000", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "470 Shutter-IOChannel": "Dev1/ao10", "405 AO-Demo": "No", "PixelSizeUm": 1.1, "470 AO-Demo": "No", "470 Shutter-MaxVolts": "5.0000", "Vincent-D3-Name": "Vincent-D3", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FileName": "img_000000000_P-DIC_006.tif", "LudlController-Reset": "Operate", "PRIME-Exposure": "20.00", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "445-Name": "DA Shutter", "555 Shutter-Name": "AnalogIO", "520 Shutter-TriggerSampleRate": "100000", "445 Shutter-IOChannel": "Dev1/ao9", "PRIME-TransposeCorrection": "0", "XYStage-StepSize": "0.1000", "445-Description": "DA device that is used as a shutter", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 AO-IOChannel": "Dev1/ao7", "520 Shutter-Name": "AnalogIO", "COM3-DataBits": "8", "640 AO-Volts": "0.0000", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "555 Shutter-IOChannel": "Dev1/ao13", "WaitInterval": null, "520 Shutter-Sequenceable": "1", "YPositionUm": 20690.300000000003, "445-State": "0", "445-DA Device": "445 Shutter", "PRIME-CircularBufferAutoSize": "ON", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "555 AO-TriggerSampleRate": "100000", "445 Shutter-Volts": "0.0000", "XLIGHT Hub-Port": "COM3", "Core-AutoFocus": "", "COM4-Handshaking": "Off", "528 Shutter-MinVolts": "0.0000", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "445 Shutter-Name": "AnalogIO", "528 Shutter-Sequenceable": "1", "XYStage-TransposeMirrorX": "0", "XYStage-TransposeMirrorY": "0", "PRIME-ReadoutRate": "100MHz 16bit", "XYStage-Name": "Stage", "640 AO-SupportsTriggering": "0", "640-Name": "DA Shutter", "640 Shutter-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "640 AO-Block voltage": "false", "445 Shutter-Sequenceable": "1", "470 AO-MaxVolts": "5.0000", "COM4-BaudRate": "9600", "470-Description": "DA device that is used as a shutter", "520 Shutter-Description": "NI DAC", "PRIME-CCDTemperature": "-14.9600", "Time": "2019-12-17 21:59:08 -0500", "640 AO-TriggerSequenceLength": "1000", "470 AO-Name": "AnalogIO", "XLIGHT Disk slider-Label": "Disk Pos 40um", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "Binning": "1x1", "TE2000-ModelType": "1", "COM3-AnswerTimeout": "500.0000", "555 Shutter-SupportsTriggering": "0", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "TE2000-Port": "COM4", "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Description": "NI DAC", "COM4-AnswerTimeout": "500.0000", "COM1-DelayBetweenCharsMs": "0.0000", "FilterBlock-Label": "Confocal", "445 AO-Name": "AnalogIO", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-Timing-ReadoutTimeNs": "24672000.0000", "528 AO-Demo": "No", "520 AO-Sequenceable": "1", "555-Description": "DA device that is used as a shutter", "405-DA Device": "405 Shutter", "405 AO-TriggerSequenceLength": "1000", "640 AO-MinVolts": "0.0000", "640 Shutter-Block voltage": "false", "FrameIndex": 0, "PRIME-Port": "CMOS", "555 AO-MinVolts": "0.0000", "COM3-Description": "Serial port driver (boost:asio)", "528 AO-Description": "NI DAC", "555 AO-IOChannel": "Dev1/ao6", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "555 AO-TriggerInputLine": "/Dev1/PFI0", "PRIME-ActualInterval-ms": "374.3700", "LudlController-Port": "COM9", "445 Shutter-Demo": "No", "PRIME-Trigger-Expose Out-Mux": "1", "SliceIndex": 6, "PRIME-CCDTemperatureSetPoint": "-15.0000", "405 Shutter-MinVolts": "0.0000", "PRIME-ExposeOutMode": "First Row", "AxisPositions": null, "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Volts": "0.0000", "528-Name": "DA Shutter", "555-State": "0", "XLIGHT Dichroic-Label": "Di03-R635-t1", "445 AO-Sequenceable": "1", "405-Name": "DA Shutter", "405 AO-IOChannel": "Dev1/ao0", "Core-Camera": "PRIME", "Focus-Name": "Focus", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "405 Shutter-IOChannel": "Dev1/ao8", "640 AO-Demo": "No", "640 Shutter-SupportsTriggering": "0", "445 AO-Demo": "No", "555-DA Device": "555 Shutter", "470 Shutter-Description": "NI DAC", "528 AO-Volts": "0.0000", "COM1-DataBits": "8", "Vincent-D3-Address": "x", "520 AO-SupportsTriggering": "0", "405-Description": "DA device that is used as a shutter", "640 Shutter-MinVolts": "0.0000", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "405 AO-Name": "AnalogIO", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "640 AO-TriggerInputLine": "/Dev1/PFI0", "405 AO-TriggerInputLine": "/Dev1/PFI0", "640-Description": "DA device that is used as a shutter", "445 Shutter-TriggerSampleRate": "100000", "470 Shutter-TriggerSequenceLength": "1000", "COM1-StopBits": "1", "520 Shutter-SupportsTriggering": "0", "405-State": "0", "Core-Initialize": "1", "XLIGHT Emission Wheel-State": "7", "520 AO-Description": "NI DAC", "528 AO-TriggerInputLine": "/Dev1/PFI0", "445 Shutter-MaxVolts": "5.0000", "COM9-Handshaking": "Off", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "XLIGHT Spin motor-Label": "On (spinning)", "PRIME-PP 5 ENABLED": "No", "UUID": "b6f0a343-94c8-4d4a-ad8e-fdbbb1e53d8e", "PRIME-TriggerMode": "Internal Trigger", "COM1-Handshaking": "Off", "PRIME-Timing-PostTriggerDelayNs": "49344000.0000", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "Focus-Position": "16479.8000", "528 Shutter-Block voltage": "false", "Channel": "P-DIC", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "555 Shutter-Demo": "No", "405 Shutter-Demo": "No", "528 AO-Name": "AnalogIO", "520-State": "0", "Core-Galvo": "", "405 Shutter-TriggerSampleRate": "100000", "Focus-FocusStepSize_(nm)": "100", "PRIME-PixelType": "16bit", "COM4-DataBits": "8", "470 AO-IOChannel": "Dev1/ao2", "520 AO-MinVolts": "0.0000", "COM9-Parity": "None", "PRIME-CircularBufferEnabled": "ON", "PRIME-PP 6 Reset": "No", "640 AO-Description": "NI DAC", "Source": "PRIME", "445 AO-MinVolts": "0.0000", "PIEZO-Name": "Stage", "445 AO-Description": "NI DAC", "PRIME-CircularBufferFrameCount": "46", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "520 AO-Volts": "0.0000", "528 Shutter-TriggerSampleRate": "100000", "XPositionUm": 62321, "OpticalPath-State": "4", "PRIME-PP 3 THRESHOLD": "80", "PRIME-PVCAM Version": "3.7.1", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Gain": "1-HDR", "640 Shutter-TriggerSequenceLength": "1000", "528 AO-SupportsTriggering": "0", "445 AO-TriggerInputLine": "/Dev1/PFI0", "OpticalPath-Name": "OpticalPath", "PRIME-PP 4 ENABLED": "Yes", "PRIME-PVCAM Adapter Version": "1.3.29", "COM3-Name": "COM3", "405 AO-MaxVolts": "5.0000", "520 Shutter-Block voltage": "false", "PRIME-Timing-ClearingTimeNs": "49344000.0000", "Core-Focus": "PIEZO", "528 AO-Block voltage": "false", "TE2000-Description": "Nikon TE2000 hub", "528 AO-MinVolts": "0.0000", "COM4-Parity": "None", "Summary": { "Slices": 11, "ChContrastMin": [ 0, 0, 0, 0, 0, 0 ], "PixelAspect": 1, "SlicesFirst": true, "Interval_ms": 0, "Time": "2019-12-17 21:58:39 -0500", "Prefix": "seq_3", "InitialPositionList": [ { "Label": "Pos0", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.435], "Focus": [16480.2], "XYStage": [ 69822.40000000001, 20100.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos1", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.425], "Focus": [16480.3], "XYStage": [ 69004, 20101 ] }, "GridColumnIndex": 0 }, { "Label": "Pos2", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.44], "Focus": [16480.1], "XYStage": [ 63119.5, 20690.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos3", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.405], "Focus": [16480.2], "XYStage": [ 62321, 20690.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos4", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.415], "Focus": [16480], "XYStage": [ 56604.100000000006, 20688.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos5", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.42000000000002], "Focus": [16480.1], "XYStage": [ 56034.200000000004, 20688.800000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos6", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.455], "Focus": [16480], "XYStage": [ 50889.3, 20496.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos7", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.43], "Focus": [16480.2], "XYStage": [ 50101.600000000006, 20496.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos8", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.67000000000002], "Focus": [16479.9], "XYStage": [ 44818.600000000006, 20496.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos9", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.67000000000002], "Focus": [16480], "XYStage": [ 43897.9, 20436.800000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos10", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.675], "Focus": [16479.9], "XYStage": [ 39670.600000000006, 20664.9 ] }, "GridColumnIndex": 0 }, { "Label": "Pos11", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.69], "Focus": [16479.9], "XYStage": [ 38734, 20664.600000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos12", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.695], "Focus": [16480.1], "XYStage": [ 34037.200000000004, 20664.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos13", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.685], "Focus": [16480.1], "XYStage": [ 33152, 20664.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos14", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.69], "Focus": [16480.1], "XYStage": [ 28122.800000000003, 20664.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos15", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.675], "Focus": [16480], "XYStage": [ 27422.9, 20664.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos16", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.64000000000001], "Focus": [16480], "XYStage": [ 23005.2, 20664.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos17", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.69], "Focus": [16479.9], "XYStage": [ 21820.600000000002, 20409.600000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos18", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.41], "Focus": [16480.1], "XYStage": [ 73697.6, -13222.800000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos19", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.455], "Focus": [16480.1], "XYStage": [ 72765.8, -13223 ] }, "GridColumnIndex": 0 }, { "Label": "Pos20", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.4], "Focus": [16480.2], "XYStage": [ 67445.5, -12303.800000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos21", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.415], "Focus": [16480.2], "XYStage": [ 66613.3, -12303.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos22", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.415], "Focus": [16480.1], "XYStage": [ 61819.4, -11936.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos23", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.405], "Focus": [16480.2], "XYStage": [ 61325.700000000004, -11937.1 ] }, "GridColumnIndex": 0 }, { "Label": "Pos24", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.46], "Focus": [16480.2], "XYStage": [ 55377, -10974.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos25", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.445], "Focus": [16480.1], "XYStage": [ 54827, -11214.800000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos26", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.435], "Focus": [16480.2], "XYStage": [ 49937.100000000006, -10919.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos27", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.425], "Focus": [16480.1], "XYStage": [ 48764, -10919.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos28", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.42000000000002], "Focus": [16480.2], "XYStage": [ 43978.4, -10920.400000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos29", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.435], "Focus": [16480.2], "XYStage": [ 43106.3, -10920.400000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos30", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.405], "Focus": [16480.3], "XYStage": [ 38212.4, -10920.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos31", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.445], "Focus": [16480.2], "XYStage": [ 37254.9, -10920.400000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos32", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.44], "Focus": [16480.3], "XYStage": [ 32472.800000000003, -11294.400000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos33", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [166.95000000000002], "Focus": [16480.2], "XYStage": [ 31420.7, -11294.400000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos34", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [166.95000000000002], "Focus": [16480.2], "XYStage": [ 26881.5, -11640 ] }, "GridColumnIndex": 0 }, { "Label": "Pos35", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [166.98], "Focus": [16480.2], "XYStage": [ 25876.100000000002, -11641.1 ] }, "GridColumnIndex": 0 } ], "z-step_um": 10, "GridRow": 0, "BitDepth": 16, "Depth": 2, "Channels": 6, "Source": "Micro-Manager", "ChColors": [ -16711681, -256, -65281, -1, -1, -16776961 ], "MetadataVersion": 10, "KeepShutterOpenChannels": true, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536, 65536 ], "CustomIntervals_ms": [], "Height": 1200, "MicroManagerVersion": "1.4.23 20180219", "GridColumn": 0, "UUID": "8c8f9d20-b128-4577-9f91-89e70f22d319", "PixelType": "GRAY16", "Width": 1200, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\Yan\\20191217-YC113-S5S6", "Frames": 1, "Comment": "", "UserName": "Nikon", "PixelSize_um": 1.1, "ROI": [ 0, 0, 1200, 1200 ], "Date": "2019-12-17", "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC", "P-DAPI-high" ], "KeepShutterOpenSlices": true, "IJType": 1, "ComputerName": "ZadorConfocal2", "Positions": 36, "PositionIndex": 3, "CameraTimeout": 20000, "TimeFirst": false }, "640 AO-Name": "AnalogIO", "Vincent-D3-Command": "Close" }, "FrameKey-0-4-7": { "COM4-StopBits": "1", "528 AO-MaxVolts": "5.0000", "XLIGHT Excitation Wheel-State": "2", "470 Shutter-Name": "AnalogIO", "Core-Shutter": "Vincent-D3", "555 AO-Demo": "No", "XLIGHT Dichroic-State": "2", "XYStage-StepSize-Y": "0.1000", "ZPositionUm": 197.405, "XYStage-StepSize-X": "0.1000", "PRIME-FirmwareVersion": "20.28", "PRIME-ClearCycles": "2", "520-Name": "DA Shutter", "405 Shutter-Name": "AnalogIO", "PIEZO-StepSize": "0.0050", "PRIME-ClearMode": "Pre-Exposure", "470 AO-MinVolts": "0.0000", "405 AO-SupportsTriggering": "1", "CameraChannelIndex": 0, "XYStage-StartSpeed": "500.0000", "COM9-StopBits": "2", "405 AO-Description": "NI DAC", "COM4-DelayBetweenCharsMs": "0.0000", "445 AO-Block voltage": "false", "520 AO-TriggerInputLine": "/Dev1/PFI0", "555 AO-Description": "NI DAC", "520 AO-TriggerSampleRate": "100000", "COM4-Verbose": "1", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM4-Name": "COM4", "405 Shutter-Volts": "0.0000", "Vincent-D3-Port": "COM1", "PRIME-PP 4 MIN ADU AFFECTED": "200", "470 Shutter-MinVolts": "0.0000", "PRIME-PP 3 ENABLED": "Yes", "470 AO-Description": "NI DAC", "PRIME-Description": "PVCAM API device adapter", "520 Shutter-MaxVolts": "5.0000", "520 Shutter-Volts": "0.0000", "528 AO-Sequenceable": "1", "PRIME-Binning": "1x1", "555 Shutter-MaxVolts": "5.0000", "528 Shutter-TriggerSequenceLength": "1000", "405 Shutter-Block voltage": "false", "XLIGHT Spin motor-State": "1", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "Vincent-D3-Time to open (ms)": "35.0000", "Vincent-D3-Time to close (ms)": "35.0000", "445 AO-TriggerSampleRate": "100000", "640 Shutter-IOChannel": "Dev1/ao14", "555 AO-Sequenceable": "1", "520 AO-Name": "AnalogIO", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "470 AO-Block voltage": "false", "COM1-Verbose": "1", "COM9-AnswerTimeout": "10000.0000", "528 Shutter-Name": "AnalogIO", "PRIME-Trigger Timeout (secs)": "10", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "NextFrame": 0, "470 AO-TriggerSequenceLength": "1000", "PRIME-TransposeXY": "0", "445 AO-TriggerSequenceLength": "1000", "COM1-Name": "COM1", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "COM9-DataBits": "8", "COM1-AnswerTimeout": "500.0000", "Camera": "", "TE2000-Version": "V2.50-V2.50-V2.10", "528 Shutter-Volts": "0.0000", "XLIGHT Hub-Name": "XLIGHT Hub", "PIEZO-Description": "Ludl stage driver adapter", "PositionName": "Pos3", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSampleRate": "100000", "PRIME-FanSpeedSetpoint": "High", "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "405 AO-TriggerSampleRate": "100000", "470 Shutter-Block voltage": "false", "470-State": "0", "COM3-Parity": "None", "405 AO-Volts": "0.0000", "PRIME-TransposeMirrorY": "0", "PRIME-ChipName": "GS144BSI", "470 AO-Volts": "0.0000", "PRIME-TransposeMirrorX": "0", "445 Shutter-SupportsTriggering": "0", "470 Shutter-SupportsTriggering": "0", "XLIGHT Hub-Description": "XLIGHT hub", "PRIME-PreampOffLimit": "10000", "470 AO-Sequenceable": "1", "528 Shutter-MaxVolts": "5.0000", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "Core-XYStage": "XYStage", "470 AO-TriggerSampleRate": "100000", "PRIME-Timing-PreTriggerDelayNs": "24672000.0000", "528-DA Device": "528 Shutter", "Focus-Description": "Nikon TE2000 focus adapter", "FilterBlock-State": "5", "520 AO-Demo": "No", "COM3-DelayBetweenCharsMs": "0.0000", "470 Shutter-Volts": "0.0000", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "PRIME-PP 2 THRESHOLD": "125", "PRIME-PP 4 THRESHOLD": "75", "XYStage-Acceleration": "4.0000", "470 Shutter-Demo": "No", "PRIME-Name": "PMPCIECam00", "640-DA Device": "640 Shutter", "405 AO-Block voltage": "false", "ElapsedTime-ms": 161751, "PRIME-Offset": "100", "445 AO-IOChannel": "Dev1/ao1", "640 AO-MaxVolts": "5.0000", "PositionIndex": 3, "PRIME-PP 1 ENABLED": "Yes", "COM1-BaudRate": "9600", "640-State": "0", "COM3-Verbose": "1", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "LudlController-TransmissionDelay": "0", "PRIME-FullWellCapacity": "62000", "PRIME-SMARTStreamingEnabled": "No", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "640 Shutter-Description": "NI DAC", "528-Description": "DA device that is used as a shutter", "COM4-Description": "Serial port driver (boost:asio)", "405 Shutter-Description": "NI DAC", "TE2000-Name": "TE2000", "528 Shutter-Description": "NI DAC", "COM9-Description": "Serial port driver (boost:asio)", "LudlController-Name": "LudlController", "445 Shutter-TriggerSequenceLength": "1000", "PRIME-SerialNumber": "A18B203008", "445 AO-Volts": "0.0000", "PRIME-X-dimension": "1200", "PRIME-ShutterCloseDelay": "0", "XLIGHT Disk slider-State": "2", "Position": "Default", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 AO-IOChannel": "Dev1/ao5", "520 AO-IOChannel": "Dev1/ao4", "528 AO-TriggerSequenceLength": "1000", "405 Shutter-MaxVolts": "5.0000", "470-DA Device": "470 Shutter", "PRIME-FTCapable": "No", "Core-ChannelGroup": "Channels", "555 AO-SupportsTriggering": "0", "PRIME-Y-dimension": "1200", "Frame": 0, "555 Shutter-Sequenceable": "1", "PRIME-Timing-ExposureTimeNs": "9992000.0000", "Core-ImageProcessor": "", "528-State": "0", "520 AO-Block voltage": "false", "XLIGHT Excitation Wheel-Label": "Excitation-2", "640 Shutter-Demo": "No", "COM3-StopBits": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "520 Shutter-IOChannel": "Dev1/ao11", "Width": 1200, "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-SupportsTriggering": "0", "640 AO-TriggerSampleRate": "100000", "520-Description": "DA device that is used as a shutter", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "405 Shutter-Sequenceable": "1", "405 Shutter-SupportsTriggering": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-TriggerSampleRate": "100000", "640 Shutter-MaxVolts": "5.0000", "555 AO-MaxVolts": "5.0000", "520 AO-TriggerSequenceLength": "1000", "COM3-BaudRate": "9600", "COM9-BaudRate": "9600", "Vincent-D3-Channel #": "Ch. #1", "PRIME-PMode": "Normal", "OpticalPath-Label": "Left(100)", "BitDepth": 16, "555 AO-TriggerSequenceLength": "1000", "445 Shutter-Description": "NI DAC", "405 AO-Sequenceable": "1", "PRIME-AcquisitionMethod": "Callbacks", "555 AO-Name": "AnalogIO", "PRIME-ShutterMode": "Pre-Exposure", "COM3-Handshaking": "Off", "445 AO-SupportsTriggering": "0", "ROI": "0-0-1200-1200", "COM9-Verbose": "1", "PRIME-Color": "OFF", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "PRIME-ShutterOpenDelay": "0", "COM1-Parity": "None", "Slice": 7, "SlicePosition": 20, "COM9-DelayBetweenCharsMs": "0.0000", "555-Name": "DA Shutter", "PIEZO-LudlSingleAxisName": "B", "XLIGHT Emission Wheel-Label": "Empty", "640 Shutter-Name": "AnalogIO", "640 AO-Sequenceable": "1", "520 Shutter-Demo": "No", "528 Shutter-Demo": "No", "470-Name": "DA Shutter", "520-DA Device": "520 Shutter", "XYStage-Description": "Ludl XY stage driver adapter", "445 Shutter-MinVolts": "0.0000", "528 AO-TriggerSampleRate": "100000", "405 AO-MinVolts": "0.0000", "405 Shutter-TriggerSequenceLength": "1000", "Height": 1200, "555 AO-Block voltage": "false", "640 Shutter-Volts": "0.0000", "PixelType": "GRAY16", "445 Shutter-Block voltage": "false", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "520 Shutter-TriggerSequenceLength": "1000", "COM9-Name": "COM9", "Core-SLM": "", "555 AO-Volts": "0.0000", "555 Shutter-Block voltage": "false", "XYStage-Speed": "1800.0000", "520 AO-MaxVolts": "5.0000", "528 Shutter-SupportsTriggering": "0", "COM1-Description": "Serial port driver (boost:asio)", "PRIME-MetadataEnabled": "No", "520 Shutter-MinVolts": "0.0000", "470 Shutter-Sequenceable": "1", "PRIME-PreampDelay": "0", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-MinVolts": "0.0000", "ChannelIndex": 4, "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Core-AutoShutter": "1", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "Exposure-ms": 20, "PRIME-PP 2 ENABLED": "Yes", "640 Shutter-TriggerSampleRate": "100000", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "470 Shutter-IOChannel": "Dev1/ao10", "405 AO-Demo": "No", "PixelSizeUm": 1.1, "470 AO-Demo": "No", "470 Shutter-MaxVolts": "5.0000", "Vincent-D3-Name": "Vincent-D3", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FileName": "img_000000000_P-DIC_007.tif", "LudlController-Reset": "Operate", "PRIME-Exposure": "20.00", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "445-Name": "DA Shutter", "555 Shutter-Name": "AnalogIO", "520 Shutter-TriggerSampleRate": "100000", "445 Shutter-IOChannel": "Dev1/ao9", "PRIME-TransposeCorrection": "0", "XYStage-StepSize": "0.1000", "445-Description": "DA device that is used as a shutter", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 AO-IOChannel": "Dev1/ao7", "520 Shutter-Name": "AnalogIO", "COM3-DataBits": "8", "640 AO-Volts": "0.0000", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "555 Shutter-IOChannel": "Dev1/ao13", "WaitInterval": null, "520 Shutter-Sequenceable": "1", "YPositionUm": 20690.300000000003, "445-State": "0", "445-DA Device": "445 Shutter", "PRIME-CircularBufferAutoSize": "ON", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "555 AO-TriggerSampleRate": "100000", "445 Shutter-Volts": "0.0000", "XLIGHT Hub-Port": "COM3", "Core-AutoFocus": "", "COM4-Handshaking": "Off", "528 Shutter-MinVolts": "0.0000", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "445 Shutter-Name": "AnalogIO", "528 Shutter-Sequenceable": "1", "XYStage-TransposeMirrorX": "0", "XYStage-TransposeMirrorY": "0", "PRIME-ReadoutRate": "100MHz 16bit", "XYStage-Name": "Stage", "640 AO-SupportsTriggering": "0", "640-Name": "DA Shutter", "640 Shutter-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "640 AO-Block voltage": "false", "445 Shutter-Sequenceable": "1", "470 AO-MaxVolts": "5.0000", "COM4-BaudRate": "9600", "470-Description": "DA device that is used as a shutter", "520 Shutter-Description": "NI DAC", "PRIME-CCDTemperature": "-14.9600", "Time": "2019-12-17 21:59:09 -0500", "640 AO-TriggerSequenceLength": "1000", "470 AO-Name": "AnalogIO", "XLIGHT Disk slider-Label": "Disk Pos 40um", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "Binning": "1x1", "TE2000-ModelType": "1", "COM3-AnswerTimeout": "500.0000", "555 Shutter-SupportsTriggering": "0", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "TE2000-Port": "COM4", "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Description": "NI DAC", "COM4-AnswerTimeout": "500.0000", "COM1-DelayBetweenCharsMs": "0.0000", "FilterBlock-Label": "Confocal", "445 AO-Name": "AnalogIO", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-Timing-ReadoutTimeNs": "24672000.0000", "528 AO-Demo": "No", "520 AO-Sequenceable": "1", "555-Description": "DA device that is used as a shutter", "405-DA Device": "405 Shutter", "405 AO-TriggerSequenceLength": "1000", "640 AO-MinVolts": "0.0000", "640 Shutter-Block voltage": "false", "FrameIndex": 0, "PRIME-Port": "CMOS", "555 AO-MinVolts": "0.0000", "COM3-Description": "Serial port driver (boost:asio)", "528 AO-Description": "NI DAC", "555 AO-IOChannel": "Dev1/ao6", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "555 AO-TriggerInputLine": "/Dev1/PFI0", "PRIME-ActualInterval-ms": "374.3700", "LudlController-Port": "COM9", "445 Shutter-Demo": "No", "PRIME-Trigger-Expose Out-Mux": "1", "SliceIndex": 7, "PRIME-CCDTemperatureSetPoint": "-15.0000", "405 Shutter-MinVolts": "0.0000", "PRIME-ExposeOutMode": "First Row", "AxisPositions": null, "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Volts": "0.0000", "528-Name": "DA Shutter", "555-State": "0", "XLIGHT Dichroic-Label": "Di03-R635-t1", "445 AO-Sequenceable": "1", "405-Name": "DA Shutter", "405 AO-IOChannel": "Dev1/ao0", "Core-Camera": "PRIME", "Focus-Name": "Focus", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "405 Shutter-IOChannel": "Dev1/ao8", "640 AO-Demo": "No", "640 Shutter-SupportsTriggering": "0", "445 AO-Demo": "No", "555-DA Device": "555 Shutter", "470 Shutter-Description": "NI DAC", "528 AO-Volts": "0.0000", "COM1-DataBits": "8", "Vincent-D3-Address": "x", "520 AO-SupportsTriggering": "0", "405-Description": "DA device that is used as a shutter", "640 Shutter-MinVolts": "0.0000", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "405 AO-Name": "AnalogIO", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "640 AO-TriggerInputLine": "/Dev1/PFI0", "405 AO-TriggerInputLine": "/Dev1/PFI0", "640-Description": "DA device that is used as a shutter", "445 Shutter-TriggerSampleRate": "100000", "470 Shutter-TriggerSequenceLength": "1000", "COM1-StopBits": "1", "520 Shutter-SupportsTriggering": "0", "405-State": "0", "Core-Initialize": "1", "XLIGHT Emission Wheel-State": "7", "520 AO-Description": "NI DAC", "528 AO-TriggerInputLine": "/Dev1/PFI0", "445 Shutter-MaxVolts": "5.0000", "COM9-Handshaking": "Off", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "XLIGHT Spin motor-Label": "On (spinning)", "PRIME-PP 5 ENABLED": "No", "UUID": "39e4448b-dc48-4eda-99a9-793accb6b0de", "PRIME-TriggerMode": "Internal Trigger", "COM1-Handshaking": "Off", "PRIME-Timing-PostTriggerDelayNs": "49344000.0000", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "Focus-Position": "16479.8000", "528 Shutter-Block voltage": "false", "Channel": "P-DIC", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "555 Shutter-Demo": "No", "405 Shutter-Demo": "No", "528 AO-Name": "AnalogIO", "520-State": "0", "Core-Galvo": "", "405 Shutter-TriggerSampleRate": "100000", "Focus-FocusStepSize_(nm)": "100", "PRIME-PixelType": "16bit", "COM4-DataBits": "8", "470 AO-IOChannel": "Dev1/ao2", "520 AO-MinVolts": "0.0000", "COM9-Parity": "None", "PRIME-CircularBufferEnabled": "ON", "PRIME-PP 6 Reset": "No", "640 AO-Description": "NI DAC", "Source": "PRIME", "445 AO-MinVolts": "0.0000", "PIEZO-Name": "Stage", "445 AO-Description": "NI DAC", "PRIME-CircularBufferFrameCount": "46", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "520 AO-Volts": "0.0000", "528 Shutter-TriggerSampleRate": "100000", "XPositionUm": 62321, "OpticalPath-State": "4", "PRIME-PP 3 THRESHOLD": "80", "PRIME-PVCAM Version": "3.7.1", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Gain": "1-HDR", "640 Shutter-TriggerSequenceLength": "1000", "528 AO-SupportsTriggering": "0", "445 AO-TriggerInputLine": "/Dev1/PFI0", "OpticalPath-Name": "OpticalPath", "PRIME-PP 4 ENABLED": "Yes", "PRIME-PVCAM Adapter Version": "1.3.29", "COM3-Name": "COM3", "405 AO-MaxVolts": "5.0000", "520 Shutter-Block voltage": "false", "PRIME-Timing-ClearingTimeNs": "49344000.0000", "Core-Focus": "PIEZO", "528 AO-Block voltage": "false", "TE2000-Description": "Nikon TE2000 hub", "528 AO-MinVolts": "0.0000", "COM4-Parity": "None", "Summary": { "Slices": 11, "ChContrastMin": [ 0, 0, 0, 0, 0, 0 ], "PixelAspect": 1, "SlicesFirst": true, "Interval_ms": 0, "Time": "2019-12-17 21:58:39 -0500", "Prefix": "seq_3", "InitialPositionList": [ { "Label": "Pos0", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.435], "Focus": [16480.2], "XYStage": [ 69822.40000000001, 20100.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos1", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.425], "Focus": [16480.3], "XYStage": [ 69004, 20101 ] }, "GridColumnIndex": 0 }, { "Label": "Pos2", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.44], "Focus": [16480.1], "XYStage": [ 63119.5, 20690.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos3", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.405], "Focus": [16480.2], "XYStage": [ 62321, 20690.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos4", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.415], "Focus": [16480], "XYStage": [ 56604.100000000006, 20688.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos5", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.42000000000002], "Focus": [16480.1], "XYStage": [ 56034.200000000004, 20688.800000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos6", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.455], "Focus": [16480], "XYStage": [ 50889.3, 20496.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos7", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.43], "Focus": [16480.2], "XYStage": [ 50101.600000000006, 20496.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos8", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.67000000000002], "Focus": [16479.9], "XYStage": [ 44818.600000000006, 20496.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos9", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.67000000000002], "Focus": [16480], "XYStage": [ 43897.9, 20436.800000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos10", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.675], "Focus": [16479.9], "XYStage": [ 39670.600000000006, 20664.9 ] }, "GridColumnIndex": 0 }, { "Label": "Pos11", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.69], "Focus": [16479.9], "XYStage": [ 38734, 20664.600000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos12", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.695], "Focus": [16480.1], "XYStage": [ 34037.200000000004, 20664.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos13", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.685], "Focus": [16480.1], "XYStage": [ 33152, 20664.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos14", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.69], "Focus": [16480.1], "XYStage": [ 28122.800000000003, 20664.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos15", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.675], "Focus": [16480], "XYStage": [ 27422.9, 20664.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos16", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.64000000000001], "Focus": [16480], "XYStage": [ 23005.2, 20664.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos17", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.69], "Focus": [16479.9], "XYStage": [ 21820.600000000002, 20409.600000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos18", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.41], "Focus": [16480.1], "XYStage": [ 73697.6, -13222.800000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos19", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.455], "Focus": [16480.1], "XYStage": [ 72765.8, -13223 ] }, "GridColumnIndex": 0 }, { "Label": "Pos20", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.4], "Focus": [16480.2], "XYStage": [ 67445.5, -12303.800000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos21", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.415], "Focus": [16480.2], "XYStage": [ 66613.3, -12303.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos22", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.415], "Focus": [16480.1], "XYStage": [ 61819.4, -11936.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos23", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.405], "Focus": [16480.2], "XYStage": [ 61325.700000000004, -11937.1 ] }, "GridColumnIndex": 0 }, { "Label": "Pos24", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.46], "Focus": [16480.2], "XYStage": [ 55377, -10974.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos25", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.445], "Focus": [16480.1], "XYStage": [ 54827, -11214.800000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos26", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.435], "Focus": [16480.2], "XYStage": [ 49937.100000000006, -10919.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos27", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.425], "Focus": [16480.1], "XYStage": [ 48764, -10919.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos28", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.42000000000002], "Focus": [16480.2], "XYStage": [ 43978.4, -10920.400000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos29", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.435], "Focus": [16480.2], "XYStage": [ 43106.3, -10920.400000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos30", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.405], "Focus": [16480.3], "XYStage": [ 38212.4, -10920.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos31", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.445], "Focus": [16480.2], "XYStage": [ 37254.9, -10920.400000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos32", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.44], "Focus": [16480.3], "XYStage": [ 32472.800000000003, -11294.400000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos33", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [166.95000000000002], "Focus": [16480.2], "XYStage": [ 31420.7, -11294.400000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos34", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [166.95000000000002], "Focus": [16480.2], "XYStage": [ 26881.5, -11640 ] }, "GridColumnIndex": 0 }, { "Label": "Pos35", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [166.98], "Focus": [16480.2], "XYStage": [ 25876.100000000002, -11641.1 ] }, "GridColumnIndex": 0 } ], "z-step_um": 10, "GridRow": 0, "BitDepth": 16, "Depth": 2, "Channels": 6, "Source": "Micro-Manager", "ChColors": [ -16711681, -256, -65281, -1, -1, -16776961 ], "MetadataVersion": 10, "KeepShutterOpenChannels": true, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536, 65536 ], "CustomIntervals_ms": [], "Height": 1200, "MicroManagerVersion": "1.4.23 20180219", "GridColumn": 0, "UUID": "8c8f9d20-b128-4577-9f91-89e70f22d319", "PixelType": "GRAY16", "Width": 1200, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\Yan\\20191217-YC113-S5S6", "Frames": 1, "Comment": "", "UserName": "Nikon", "PixelSize_um": 1.1, "ROI": [ 0, 0, 1200, 1200 ], "Date": "2019-12-17", "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC", "P-DAPI-high" ], "KeepShutterOpenSlices": true, "IJType": 1, "ComputerName": "ZadorConfocal2", "Positions": 36, "PositionIndex": 3, "CameraTimeout": 20000, "TimeFirst": false }, "640 AO-Name": "AnalogIO", "Vincent-D3-Command": "Close" }, "FrameKey-0-4-8": { "COM4-StopBits": "1", "528 AO-MaxVolts": "5.0000", "XLIGHT Excitation Wheel-State": "2", "470 Shutter-Name": "AnalogIO", "Core-Shutter": "Vincent-D3", "555 AO-Demo": "No", "XLIGHT Dichroic-State": "2", "XYStage-StepSize-Y": "0.1000", "ZPositionUm": 207.405, "XYStage-StepSize-X": "0.1000", "PRIME-FirmwareVersion": "20.28", "PRIME-ClearCycles": "2", "520-Name": "DA Shutter", "405 Shutter-Name": "AnalogIO", "PIEZO-StepSize": "0.0050", "PRIME-ClearMode": "Pre-Exposure", "470 AO-MinVolts": "0.0000", "405 AO-SupportsTriggering": "1", "CameraChannelIndex": 0, "XYStage-StartSpeed": "500.0000", "COM9-StopBits": "2", "405 AO-Description": "NI DAC", "COM4-DelayBetweenCharsMs": "0.0000", "445 AO-Block voltage": "false", "520 AO-TriggerInputLine": "/Dev1/PFI0", "555 AO-Description": "NI DAC", "520 AO-TriggerSampleRate": "100000", "COM4-Verbose": "1", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM4-Name": "COM4", "405 Shutter-Volts": "0.0000", "Vincent-D3-Port": "COM1", "PRIME-PP 4 MIN ADU AFFECTED": "200", "470 Shutter-MinVolts": "0.0000", "PRIME-PP 3 ENABLED": "Yes", "470 AO-Description": "NI DAC", "PRIME-Description": "PVCAM API device adapter", "520 Shutter-MaxVolts": "5.0000", "520 Shutter-Volts": "0.0000", "528 AO-Sequenceable": "1", "PRIME-Binning": "1x1", "555 Shutter-MaxVolts": "5.0000", "528 Shutter-TriggerSequenceLength": "1000", "405 Shutter-Block voltage": "false", "XLIGHT Spin motor-State": "1", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "Vincent-D3-Time to open (ms)": "35.0000", "Vincent-D3-Time to close (ms)": "35.0000", "445 AO-TriggerSampleRate": "100000", "640 Shutter-IOChannel": "Dev1/ao14", "555 AO-Sequenceable": "1", "520 AO-Name": "AnalogIO", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "470 AO-Block voltage": "false", "COM1-Verbose": "1", "COM9-AnswerTimeout": "10000.0000", "528 Shutter-Name": "AnalogIO", "PRIME-Trigger Timeout (secs)": "10", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "NextFrame": 0, "470 AO-TriggerSequenceLength": "1000", "PRIME-TransposeXY": "0", "445 AO-TriggerSequenceLength": "1000", "COM1-Name": "COM1", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "COM9-DataBits": "8", "COM1-AnswerTimeout": "500.0000", "Camera": "", "TE2000-Version": "V2.50-V2.50-V2.10", "528 Shutter-Volts": "0.0000", "XLIGHT Hub-Name": "XLIGHT Hub", "PIEZO-Description": "Ludl stage driver adapter", "PositionName": "Pos3", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSampleRate": "100000", "PRIME-FanSpeedSetpoint": "High", "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "405 AO-TriggerSampleRate": "100000", "470 Shutter-Block voltage": "false", "470-State": "0", "COM3-Parity": "None", "405 AO-Volts": "0.0000", "PRIME-TransposeMirrorY": "0", "PRIME-ChipName": "GS144BSI", "470 AO-Volts": "0.0000", "PRIME-TransposeMirrorX": "0", "445 Shutter-SupportsTriggering": "0", "470 Shutter-SupportsTriggering": "0", "XLIGHT Hub-Description": "XLIGHT hub", "PRIME-PreampOffLimit": "10000", "470 AO-Sequenceable": "1", "528 Shutter-MaxVolts": "5.0000", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "Core-XYStage": "XYStage", "470 AO-TriggerSampleRate": "100000", "PRIME-Timing-PreTriggerDelayNs": "24672000.0000", "528-DA Device": "528 Shutter", "Focus-Description": "Nikon TE2000 focus adapter", "FilterBlock-State": "5", "520 AO-Demo": "No", "COM3-DelayBetweenCharsMs": "0.0000", "470 Shutter-Volts": "0.0000", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "PRIME-PP 2 THRESHOLD": "125", "PRIME-PP 4 THRESHOLD": "75", "XYStage-Acceleration": "4.0000", "470 Shutter-Demo": "No", "PRIME-Name": "PMPCIECam00", "640-DA Device": "640 Shutter", "405 AO-Block voltage": "false", "ElapsedTime-ms": 162012, "PRIME-Offset": "100", "445 AO-IOChannel": "Dev1/ao1", "640 AO-MaxVolts": "5.0000", "PositionIndex": 3, "PRIME-PP 1 ENABLED": "Yes", "COM1-BaudRate": "9600", "640-State": "0", "COM3-Verbose": "1", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "LudlController-TransmissionDelay": "0", "PRIME-FullWellCapacity": "62000", "PRIME-SMARTStreamingEnabled": "No", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "640 Shutter-Description": "NI DAC", "528-Description": "DA device that is used as a shutter", "COM4-Description": "Serial port driver (boost:asio)", "405 Shutter-Description": "NI DAC", "TE2000-Name": "TE2000", "528 Shutter-Description": "NI DAC", "COM9-Description": "Serial port driver (boost:asio)", "LudlController-Name": "LudlController", "445 Shutter-TriggerSequenceLength": "1000", "PRIME-SerialNumber": "A18B203008", "445 AO-Volts": "0.0000", "PRIME-X-dimension": "1200", "PRIME-ShutterCloseDelay": "0", "XLIGHT Disk slider-State": "2", "Position": "Default", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 AO-IOChannel": "Dev1/ao5", "520 AO-IOChannel": "Dev1/ao4", "528 AO-TriggerSequenceLength": "1000", "405 Shutter-MaxVolts": "5.0000", "470-DA Device": "470 Shutter", "PRIME-FTCapable": "No", "Core-ChannelGroup": "Channels", "555 AO-SupportsTriggering": "0", "PRIME-Y-dimension": "1200", "Frame": 0, "555 Shutter-Sequenceable": "1", "PRIME-Timing-ExposureTimeNs": "9992000.0000", "Core-ImageProcessor": "", "528-State": "0", "520 AO-Block voltage": "false", "XLIGHT Excitation Wheel-Label": "Excitation-2", "640 Shutter-Demo": "No", "COM3-StopBits": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "520 Shutter-IOChannel": "Dev1/ao11", "Width": 1200, "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-SupportsTriggering": "0", "640 AO-TriggerSampleRate": "100000", "520-Description": "DA device that is used as a shutter", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "405 Shutter-Sequenceable": "1", "405 Shutter-SupportsTriggering": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-TriggerSampleRate": "100000", "640 Shutter-MaxVolts": "5.0000", "555 AO-MaxVolts": "5.0000", "520 AO-TriggerSequenceLength": "1000", "COM3-BaudRate": "9600", "COM9-BaudRate": "9600", "Vincent-D3-Channel #": "Ch. #1", "PRIME-PMode": "Normal", "OpticalPath-Label": "Left(100)", "BitDepth": 16, "555 AO-TriggerSequenceLength": "1000", "445 Shutter-Description": "NI DAC", "405 AO-Sequenceable": "1", "PRIME-AcquisitionMethod": "Callbacks", "555 AO-Name": "AnalogIO", "PRIME-ShutterMode": "Pre-Exposure", "COM3-Handshaking": "Off", "445 AO-SupportsTriggering": "0", "ROI": "0-0-1200-1200", "COM9-Verbose": "1", "PRIME-Color": "OFF", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "PRIME-ShutterOpenDelay": "0", "COM1-Parity": "None", "Slice": 8, "SlicePosition": 30, "COM9-DelayBetweenCharsMs": "0.0000", "555-Name": "DA Shutter", "PIEZO-LudlSingleAxisName": "B", "XLIGHT Emission Wheel-Label": "Empty", "640 Shutter-Name": "AnalogIO", "640 AO-Sequenceable": "1", "520 Shutter-Demo": "No", "528 Shutter-Demo": "No", "470-Name": "DA Shutter", "520-DA Device": "520 Shutter", "XYStage-Description": "Ludl XY stage driver adapter", "445 Shutter-MinVolts": "0.0000", "528 AO-TriggerSampleRate": "100000", "405 AO-MinVolts": "0.0000", "405 Shutter-TriggerSequenceLength": "1000", "Height": 1200, "555 AO-Block voltage": "false", "640 Shutter-Volts": "0.0000", "PixelType": "GRAY16", "445 Shutter-Block voltage": "false", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "520 Shutter-TriggerSequenceLength": "1000", "COM9-Name": "COM9", "Core-SLM": "", "555 AO-Volts": "0.0000", "555 Shutter-Block voltage": "false", "XYStage-Speed": "1800.0000", "520 AO-MaxVolts": "5.0000", "528 Shutter-SupportsTriggering": "0", "COM1-Description": "Serial port driver (boost:asio)", "PRIME-MetadataEnabled": "No", "520 Shutter-MinVolts": "0.0000", "470 Shutter-Sequenceable": "1", "PRIME-PreampDelay": "0", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-MinVolts": "0.0000", "ChannelIndex": 4, "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Core-AutoShutter": "1", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "Exposure-ms": 20, "PRIME-PP 2 ENABLED": "Yes", "640 Shutter-TriggerSampleRate": "100000", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "470 Shutter-IOChannel": "Dev1/ao10", "405 AO-Demo": "No", "PixelSizeUm": 1.1, "470 AO-Demo": "No", "470 Shutter-MaxVolts": "5.0000", "Vincent-D3-Name": "Vincent-D3", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FileName": "img_000000000_P-DIC_008.tif", "LudlController-Reset": "Operate", "PRIME-Exposure": "20.00", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "445-Name": "DA Shutter", "555 Shutter-Name": "AnalogIO", "520 Shutter-TriggerSampleRate": "100000", "445 Shutter-IOChannel": "Dev1/ao9", "PRIME-TransposeCorrection": "0", "XYStage-StepSize": "0.1000", "445-Description": "DA device that is used as a shutter", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 AO-IOChannel": "Dev1/ao7", "520 Shutter-Name": "AnalogIO", "COM3-DataBits": "8", "640 AO-Volts": "0.0000", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "555 Shutter-IOChannel": "Dev1/ao13", "WaitInterval": null, "520 Shutter-Sequenceable": "1", "YPositionUm": 20690.300000000003, "445-State": "0", "445-DA Device": "445 Shutter", "PRIME-CircularBufferAutoSize": "ON", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "555 AO-TriggerSampleRate": "100000", "445 Shutter-Volts": "0.0000", "XLIGHT Hub-Port": "COM3", "Core-AutoFocus": "", "COM4-Handshaking": "Off", "528 Shutter-MinVolts": "0.0000", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "445 Shutter-Name": "AnalogIO", "528 Shutter-Sequenceable": "1", "XYStage-TransposeMirrorX": "0", "XYStage-TransposeMirrorY": "0", "PRIME-ReadoutRate": "100MHz 16bit", "XYStage-Name": "Stage", "640 AO-SupportsTriggering": "0", "640-Name": "DA Shutter", "640 Shutter-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "640 AO-Block voltage": "false", "445 Shutter-Sequenceable": "1", "470 AO-MaxVolts": "5.0000", "COM4-BaudRate": "9600", "470-Description": "DA device that is used as a shutter", "520 Shutter-Description": "NI DAC", "PRIME-CCDTemperature": "-14.9600", "Time": "2019-12-17 21:59:09 -0500", "640 AO-TriggerSequenceLength": "1000", "470 AO-Name": "AnalogIO", "XLIGHT Disk slider-Label": "Disk Pos 40um", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "Binning": "1x1", "TE2000-ModelType": "1", "COM3-AnswerTimeout": "500.0000", "555 Shutter-SupportsTriggering": "0", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "TE2000-Port": "COM4", "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Description": "NI DAC", "COM4-AnswerTimeout": "500.0000", "COM1-DelayBetweenCharsMs": "0.0000", "FilterBlock-Label": "Confocal", "445 AO-Name": "AnalogIO", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-Timing-ReadoutTimeNs": "24672000.0000", "528 AO-Demo": "No", "520 AO-Sequenceable": "1", "555-Description": "DA device that is used as a shutter", "405-DA Device": "405 Shutter", "405 AO-TriggerSequenceLength": "1000", "640 AO-MinVolts": "0.0000", "640 Shutter-Block voltage": "false", "FrameIndex": 0, "PRIME-Port": "CMOS", "555 AO-MinVolts": "0.0000", "COM3-Description": "Serial port driver (boost:asio)", "528 AO-Description": "NI DAC", "555 AO-IOChannel": "Dev1/ao6", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "555 AO-TriggerInputLine": "/Dev1/PFI0", "PRIME-ActualInterval-ms": "374.3700", "LudlController-Port": "COM9", "445 Shutter-Demo": "No", "PRIME-Trigger-Expose Out-Mux": "1", "SliceIndex": 8, "PRIME-CCDTemperatureSetPoint": "-15.0000", "405 Shutter-MinVolts": "0.0000", "PRIME-ExposeOutMode": "First Row", "AxisPositions": null, "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Volts": "0.0000", "528-Name": "DA Shutter", "555-State": "0", "XLIGHT Dichroic-Label": "Di03-R635-t1", "445 AO-Sequenceable": "1", "405-Name": "DA Shutter", "405 AO-IOChannel": "Dev1/ao0", "Core-Camera": "PRIME", "Focus-Name": "Focus", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "405 Shutter-IOChannel": "Dev1/ao8", "640 AO-Demo": "No", "640 Shutter-SupportsTriggering": "0", "445 AO-Demo": "No", "555-DA Device": "555 Shutter", "470 Shutter-Description": "NI DAC", "528 AO-Volts": "0.0000", "COM1-DataBits": "8", "Vincent-D3-Address": "x", "520 AO-SupportsTriggering": "0", "405-Description": "DA device that is used as a shutter", "640 Shutter-MinVolts": "0.0000", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "405 AO-Name": "AnalogIO", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "640 AO-TriggerInputLine": "/Dev1/PFI0", "405 AO-TriggerInputLine": "/Dev1/PFI0", "640-Description": "DA device that is used as a shutter", "445 Shutter-TriggerSampleRate": "100000", "470 Shutter-TriggerSequenceLength": "1000", "COM1-StopBits": "1", "520 Shutter-SupportsTriggering": "0", "405-State": "0", "Core-Initialize": "1", "XLIGHT Emission Wheel-State": "7", "520 AO-Description": "NI DAC", "528 AO-TriggerInputLine": "/Dev1/PFI0", "445 Shutter-MaxVolts": "5.0000", "COM9-Handshaking": "Off", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "XLIGHT Spin motor-Label": "On (spinning)", "PRIME-PP 5 ENABLED": "No", "UUID": "9e7f4301-a689-48ba-b1b3-c36f8b46b133", "PRIME-TriggerMode": "Internal Trigger", "COM1-Handshaking": "Off", "PRIME-Timing-PostTriggerDelayNs": "49344000.0000", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "Focus-Position": "16479.8000", "528 Shutter-Block voltage": "false", "Channel": "P-DIC", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "555 Shutter-Demo": "No", "405 Shutter-Demo": "No", "528 AO-Name": "AnalogIO", "520-State": "0", "Core-Galvo": "", "405 Shutter-TriggerSampleRate": "100000", "Focus-FocusStepSize_(nm)": "100", "PRIME-PixelType": "16bit", "COM4-DataBits": "8", "470 AO-IOChannel": "Dev1/ao2", "520 AO-MinVolts": "0.0000", "COM9-Parity": "None", "PRIME-CircularBufferEnabled": "ON", "PRIME-PP 6 Reset": "No", "640 AO-Description": "NI DAC", "Source": "PRIME", "445 AO-MinVolts": "0.0000", "PIEZO-Name": "Stage", "445 AO-Description": "NI DAC", "PRIME-CircularBufferFrameCount": "46", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "520 AO-Volts": "0.0000", "528 Shutter-TriggerSampleRate": "100000", "XPositionUm": 62321, "OpticalPath-State": "4", "PRIME-PP 3 THRESHOLD": "80", "PRIME-PVCAM Version": "3.7.1", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Gain": "1-HDR", "640 Shutter-TriggerSequenceLength": "1000", "528 AO-SupportsTriggering": "0", "445 AO-TriggerInputLine": "/Dev1/PFI0", "OpticalPath-Name": "OpticalPath", "PRIME-PP 4 ENABLED": "Yes", "PRIME-PVCAM Adapter Version": "1.3.29", "COM3-Name": "COM3", "405 AO-MaxVolts": "5.0000", "520 Shutter-Block voltage": "false", "PRIME-Timing-ClearingTimeNs": "49344000.0000", "Core-Focus": "PIEZO", "528 AO-Block voltage": "false", "TE2000-Description": "Nikon TE2000 hub", "528 AO-MinVolts": "0.0000", "COM4-Parity": "None", "Summary": { "Slices": 11, "ChContrastMin": [ 0, 0, 0, 0, 0, 0 ], "PixelAspect": 1, "SlicesFirst": true, "Interval_ms": 0, "Time": "2019-12-17 21:58:39 -0500", "Prefix": "seq_3", "InitialPositionList": [ { "Label": "Pos0", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.435], "Focus": [16480.2], "XYStage": [ 69822.40000000001, 20100.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos1", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.425], "Focus": [16480.3], "XYStage": [ 69004, 20101 ] }, "GridColumnIndex": 0 }, { "Label": "Pos2", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.44], "Focus": [16480.1], "XYStage": [ 63119.5, 20690.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos3", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.405], "Focus": [16480.2], "XYStage": [ 62321, 20690.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos4", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.415], "Focus": [16480], "XYStage": [ 56604.100000000006, 20688.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos5", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.42000000000002], "Focus": [16480.1], "XYStage": [ 56034.200000000004, 20688.800000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos6", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.455], "Focus": [16480], "XYStage": [ 50889.3, 20496.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos7", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.43], "Focus": [16480.2], "XYStage": [ 50101.600000000006, 20496.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos8", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.67000000000002], "Focus": [16479.9], "XYStage": [ 44818.600000000006, 20496.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos9", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.67000000000002], "Focus": [16480], "XYStage": [ 43897.9, 20436.800000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos10", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.675], "Focus": [16479.9], "XYStage": [ 39670.600000000006, 20664.9 ] }, "GridColumnIndex": 0 }, { "Label": "Pos11", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.69], "Focus": [16479.9], "XYStage": [ 38734, 20664.600000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos12", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.695], "Focus": [16480.1], "XYStage": [ 34037.200000000004, 20664.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos13", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.685], "Focus": [16480.1], "XYStage": [ 33152, 20664.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos14", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.69], "Focus": [16480.1], "XYStage": [ 28122.800000000003, 20664.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos15", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.675], "Focus": [16480], "XYStage": [ 27422.9, 20664.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos16", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.64000000000001], "Focus": [16480], "XYStage": [ 23005.2, 20664.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos17", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.69], "Focus": [16479.9], "XYStage": [ 21820.600000000002, 20409.600000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos18", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.41], "Focus": [16480.1], "XYStage": [ 73697.6, -13222.800000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos19", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.455], "Focus": [16480.1], "XYStage": [ 72765.8, -13223 ] }, "GridColumnIndex": 0 }, { "Label": "Pos20", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.4], "Focus": [16480.2], "XYStage": [ 67445.5, -12303.800000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos21", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.415], "Focus": [16480.2], "XYStage": [ 66613.3, -12303.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos22", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.415], "Focus": [16480.1], "XYStage": [ 61819.4, -11936.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos23", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.405], "Focus": [16480.2], "XYStage": [ 61325.700000000004, -11937.1 ] }, "GridColumnIndex": 0 }, { "Label": "Pos24", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.46], "Focus": [16480.2], "XYStage": [ 55377, -10974.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos25", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.445], "Focus": [16480.1], "XYStage": [ 54827, -11214.800000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos26", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.435], "Focus": [16480.2], "XYStage": [ 49937.100000000006, -10919.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos27", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.425], "Focus": [16480.1], "XYStage": [ 48764, -10919.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos28", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.42000000000002], "Focus": [16480.2], "XYStage": [ 43978.4, -10920.400000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos29", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.435], "Focus": [16480.2], "XYStage": [ 43106.3, -10920.400000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos30", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.405], "Focus": [16480.3], "XYStage": [ 38212.4, -10920.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos31", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.445], "Focus": [16480.2], "XYStage": [ 37254.9, -10920.400000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos32", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.44], "Focus": [16480.3], "XYStage": [ 32472.800000000003, -11294.400000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos33", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [166.95000000000002], "Focus": [16480.2], "XYStage": [ 31420.7, -11294.400000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos34", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [166.95000000000002], "Focus": [16480.2], "XYStage": [ 26881.5, -11640 ] }, "GridColumnIndex": 0 }, { "Label": "Pos35", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [166.98], "Focus": [16480.2], "XYStage": [ 25876.100000000002, -11641.1 ] }, "GridColumnIndex": 0 } ], "z-step_um": 10, "GridRow": 0, "BitDepth": 16, "Depth": 2, "Channels": 6, "Source": "Micro-Manager", "ChColors": [ -16711681, -256, -65281, -1, -1, -16776961 ], "MetadataVersion": 10, "KeepShutterOpenChannels": true, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536, 65536 ], "CustomIntervals_ms": [], "Height": 1200, "MicroManagerVersion": "1.4.23 20180219", "GridColumn": 0, "UUID": "8c8f9d20-b128-4577-9f91-89e70f22d319", "PixelType": "GRAY16", "Width": 1200, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\Yan\\20191217-YC113-S5S6", "Frames": 1, "Comment": "", "UserName": "Nikon", "PixelSize_um": 1.1, "ROI": [ 0, 0, 1200, 1200 ], "Date": "2019-12-17", "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC", "P-DAPI-high" ], "KeepShutterOpenSlices": true, "IJType": 1, "ComputerName": "ZadorConfocal2", "Positions": 36, "PositionIndex": 3, "CameraTimeout": 20000, "TimeFirst": false }, "640 AO-Name": "AnalogIO", "Vincent-D3-Command": "Close" }, "FrameKey-0-4-9": { "COM4-StopBits": "1", "528 AO-MaxVolts": "5.0000", "XLIGHT Excitation Wheel-State": "2", "470 Shutter-Name": "AnalogIO", "Core-Shutter": "Vincent-D3", "555 AO-Demo": "No", "XLIGHT Dichroic-State": "2", "XYStage-StepSize-Y": "0.1000", "ZPositionUm": 217.405, "XYStage-StepSize-X": "0.1000", "PRIME-FirmwareVersion": "20.28", "PRIME-ClearCycles": "2", "520-Name": "DA Shutter", "405 Shutter-Name": "AnalogIO", "PIEZO-StepSize": "0.0050", "PRIME-ClearMode": "Pre-Exposure", "470 AO-MinVolts": "0.0000", "405 AO-SupportsTriggering": "1", "CameraChannelIndex": 0, "XYStage-StartSpeed": "500.0000", "COM9-StopBits": "2", "405 AO-Description": "NI DAC", "COM4-DelayBetweenCharsMs": "0.0000", "445 AO-Block voltage": "false", "520 AO-TriggerInputLine": "/Dev1/PFI0", "555 AO-Description": "NI DAC", "520 AO-TriggerSampleRate": "100000", "COM4-Verbose": "1", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM4-Name": "COM4", "405 Shutter-Volts": "0.0000", "Vincent-D3-Port": "COM1", "PRIME-PP 4 MIN ADU AFFECTED": "200", "470 Shutter-MinVolts": "0.0000", "PRIME-PP 3 ENABLED": "Yes", "470 AO-Description": "NI DAC", "PRIME-Description": "PVCAM API device adapter", "520 Shutter-MaxVolts": "5.0000", "520 Shutter-Volts": "0.0000", "528 AO-Sequenceable": "1", "PRIME-Binning": "1x1", "555 Shutter-MaxVolts": "5.0000", "528 Shutter-TriggerSequenceLength": "1000", "405 Shutter-Block voltage": "false", "XLIGHT Spin motor-State": "1", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "Vincent-D3-Time to open (ms)": "35.0000", "Vincent-D3-Time to close (ms)": "35.0000", "445 AO-TriggerSampleRate": "100000", "640 Shutter-IOChannel": "Dev1/ao14", "555 AO-Sequenceable": "1", "520 AO-Name": "AnalogIO", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "470 AO-Block voltage": "false", "COM1-Verbose": "1", "COM9-AnswerTimeout": "10000.0000", "528 Shutter-Name": "AnalogIO", "PRIME-Trigger Timeout (secs)": "10", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "NextFrame": 0, "470 AO-TriggerSequenceLength": "1000", "PRIME-TransposeXY": "0", "445 AO-TriggerSequenceLength": "1000", "COM1-Name": "COM1", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "COM9-DataBits": "8", "COM1-AnswerTimeout": "500.0000", "Camera": "", "TE2000-Version": "V2.50-V2.50-V2.10", "528 Shutter-Volts": "0.0000", "XLIGHT Hub-Name": "XLIGHT Hub", "PIEZO-Description": "Ludl stage driver adapter", "PositionName": "Pos3", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSampleRate": "100000", "PRIME-FanSpeedSetpoint": "High", "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "405 AO-TriggerSampleRate": "100000", "470 Shutter-Block voltage": "false", "470-State": "0", "COM3-Parity": "None", "405 AO-Volts": "0.0000", "PRIME-TransposeMirrorY": "0", "PRIME-ChipName": "GS144BSI", "470 AO-Volts": "0.0000", "PRIME-TransposeMirrorX": "0", "445 Shutter-SupportsTriggering": "0", "470 Shutter-SupportsTriggering": "0", "XLIGHT Hub-Description": "XLIGHT hub", "PRIME-PreampOffLimit": "10000", "470 AO-Sequenceable": "1", "528 Shutter-MaxVolts": "5.0000", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "Core-XYStage": "XYStage", "470 AO-TriggerSampleRate": "100000", "PRIME-Timing-PreTriggerDelayNs": "24672000.0000", "528-DA Device": "528 Shutter", "Focus-Description": "Nikon TE2000 focus adapter", "FilterBlock-State": "5", "520 AO-Demo": "No", "COM3-DelayBetweenCharsMs": "0.0000", "470 Shutter-Volts": "0.0000", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "PRIME-PP 2 THRESHOLD": "125", "PRIME-PP 4 THRESHOLD": "75", "XYStage-Acceleration": "4.0000", "470 Shutter-Demo": "No", "PRIME-Name": "PMPCIECam00", "640-DA Device": "640 Shutter", "405 AO-Block voltage": "false", "ElapsedTime-ms": 162263, "PRIME-Offset": "100", "445 AO-IOChannel": "Dev1/ao1", "640 AO-MaxVolts": "5.0000", "PositionIndex": 3, "PRIME-PP 1 ENABLED": "Yes", "COM1-BaudRate": "9600", "640-State": "0", "COM3-Verbose": "1", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "LudlController-TransmissionDelay": "0", "PRIME-FullWellCapacity": "62000", "PRIME-SMARTStreamingEnabled": "No", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "640 Shutter-Description": "NI DAC", "528-Description": "DA device that is used as a shutter", "COM4-Description": "Serial port driver (boost:asio)", "405 Shutter-Description": "NI DAC", "TE2000-Name": "TE2000", "528 Shutter-Description": "NI DAC", "COM9-Description": "Serial port driver (boost:asio)", "LudlController-Name": "LudlController", "445 Shutter-TriggerSequenceLength": "1000", "PRIME-SerialNumber": "A18B203008", "445 AO-Volts": "0.0000", "PRIME-X-dimension": "1200", "PRIME-ShutterCloseDelay": "0", "XLIGHT Disk slider-State": "2", "Position": "Default", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 AO-IOChannel": "Dev1/ao5", "520 AO-IOChannel": "Dev1/ao4", "528 AO-TriggerSequenceLength": "1000", "405 Shutter-MaxVolts": "5.0000", "470-DA Device": "470 Shutter", "PRIME-FTCapable": "No", "Core-ChannelGroup": "Channels", "555 AO-SupportsTriggering": "0", "PRIME-Y-dimension": "1200", "Frame": 0, "555 Shutter-Sequenceable": "1", "PRIME-Timing-ExposureTimeNs": "9992000.0000", "Core-ImageProcessor": "", "528-State": "0", "520 AO-Block voltage": "false", "XLIGHT Excitation Wheel-Label": "Excitation-2", "640 Shutter-Demo": "No", "COM3-StopBits": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "520 Shutter-IOChannel": "Dev1/ao11", "Width": 1200, "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-SupportsTriggering": "0", "640 AO-TriggerSampleRate": "100000", "520-Description": "DA device that is used as a shutter", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "405 Shutter-Sequenceable": "1", "405 Shutter-SupportsTriggering": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-TriggerSampleRate": "100000", "640 Shutter-MaxVolts": "5.0000", "555 AO-MaxVolts": "5.0000", "520 AO-TriggerSequenceLength": "1000", "COM3-BaudRate": "9600", "COM9-BaudRate": "9600", "Vincent-D3-Channel #": "Ch. #1", "PRIME-PMode": "Normal", "OpticalPath-Label": "Left(100)", "BitDepth": 16, "555 AO-TriggerSequenceLength": "1000", "445 Shutter-Description": "NI DAC", "405 AO-Sequenceable": "1", "PRIME-AcquisitionMethod": "Callbacks", "555 AO-Name": "AnalogIO", "PRIME-ShutterMode": "Pre-Exposure", "COM3-Handshaking": "Off", "445 AO-SupportsTriggering": "0", "ROI": "0-0-1200-1200", "COM9-Verbose": "1", "PRIME-Color": "OFF", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "PRIME-ShutterOpenDelay": "0", "COM1-Parity": "None", "Slice": 9, "SlicePosition": 40, "COM9-DelayBetweenCharsMs": "0.0000", "555-Name": "DA Shutter", "PIEZO-LudlSingleAxisName": "B", "XLIGHT Emission Wheel-Label": "Empty", "640 Shutter-Name": "AnalogIO", "640 AO-Sequenceable": "1", "520 Shutter-Demo": "No", "528 Shutter-Demo": "No", "470-Name": "DA Shutter", "520-DA Device": "520 Shutter", "XYStage-Description": "Ludl XY stage driver adapter", "445 Shutter-MinVolts": "0.0000", "528 AO-TriggerSampleRate": "100000", "405 AO-MinVolts": "0.0000", "405 Shutter-TriggerSequenceLength": "1000", "Height": 1200, "555 AO-Block voltage": "false", "640 Shutter-Volts": "0.0000", "PixelType": "GRAY16", "445 Shutter-Block voltage": "false", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "520 Shutter-TriggerSequenceLength": "1000", "COM9-Name": "COM9", "Core-SLM": "", "555 AO-Volts": "0.0000", "555 Shutter-Block voltage": "false", "XYStage-Speed": "1800.0000", "520 AO-MaxVolts": "5.0000", "528 Shutter-SupportsTriggering": "0", "COM1-Description": "Serial port driver (boost:asio)", "PRIME-MetadataEnabled": "No", "520 Shutter-MinVolts": "0.0000", "470 Shutter-Sequenceable": "1", "PRIME-PreampDelay": "0", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-MinVolts": "0.0000", "ChannelIndex": 4, "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Core-AutoShutter": "1", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "Exposure-ms": 20, "PRIME-PP 2 ENABLED": "Yes", "640 Shutter-TriggerSampleRate": "100000", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "470 Shutter-IOChannel": "Dev1/ao10", "405 AO-Demo": "No", "PixelSizeUm": 1.1, "470 AO-Demo": "No", "470 Shutter-MaxVolts": "5.0000", "Vincent-D3-Name": "Vincent-D3", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FileName": "img_000000000_P-DIC_009.tif", "LudlController-Reset": "Operate", "PRIME-Exposure": "20.00", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "445-Name": "DA Shutter", "555 Shutter-Name": "AnalogIO", "520 Shutter-TriggerSampleRate": "100000", "445 Shutter-IOChannel": "Dev1/ao9", "PRIME-TransposeCorrection": "0", "XYStage-StepSize": "0.1000", "445-Description": "DA device that is used as a shutter", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 AO-IOChannel": "Dev1/ao7", "520 Shutter-Name": "AnalogIO", "COM3-DataBits": "8", "640 AO-Volts": "0.0000", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "555 Shutter-IOChannel": "Dev1/ao13", "WaitInterval": null, "520 Shutter-Sequenceable": "1", "YPositionUm": 20690.300000000003, "445-State": "0", "445-DA Device": "445 Shutter", "PRIME-CircularBufferAutoSize": "ON", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "555 AO-TriggerSampleRate": "100000", "445 Shutter-Volts": "0.0000", "XLIGHT Hub-Port": "COM3", "Core-AutoFocus": "", "COM4-Handshaking": "Off", "528 Shutter-MinVolts": "0.0000", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "445 Shutter-Name": "AnalogIO", "528 Shutter-Sequenceable": "1", "XYStage-TransposeMirrorX": "0", "XYStage-TransposeMirrorY": "0", "PRIME-ReadoutRate": "100MHz 16bit", "XYStage-Name": "Stage", "640 AO-SupportsTriggering": "0", "640-Name": "DA Shutter", "640 Shutter-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "640 AO-Block voltage": "false", "445 Shutter-Sequenceable": "1", "470 AO-MaxVolts": "5.0000", "COM4-BaudRate": "9600", "470-Description": "DA device that is used as a shutter", "520 Shutter-Description": "NI DAC", "PRIME-CCDTemperature": "-14.9600", "Time": "2019-12-17 21:59:09 -0500", "640 AO-TriggerSequenceLength": "1000", "470 AO-Name": "AnalogIO", "XLIGHT Disk slider-Label": "Disk Pos 40um", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "Binning": "1x1", "TE2000-ModelType": "1", "COM3-AnswerTimeout": "500.0000", "555 Shutter-SupportsTriggering": "0", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "TE2000-Port": "COM4", "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Description": "NI DAC", "COM4-AnswerTimeout": "500.0000", "COM1-DelayBetweenCharsMs": "0.0000", "FilterBlock-Label": "Confocal", "445 AO-Name": "AnalogIO", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-Timing-ReadoutTimeNs": "24672000.0000", "528 AO-Demo": "No", "520 AO-Sequenceable": "1", "555-Description": "DA device that is used as a shutter", "405-DA Device": "405 Shutter", "405 AO-TriggerSequenceLength": "1000", "640 AO-MinVolts": "0.0000", "640 Shutter-Block voltage": "false", "FrameIndex": 0, "PRIME-Port": "CMOS", "555 AO-MinVolts": "0.0000", "COM3-Description": "Serial port driver (boost:asio)", "528 AO-Description": "NI DAC", "555 AO-IOChannel": "Dev1/ao6", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "555 AO-TriggerInputLine": "/Dev1/PFI0", "PRIME-ActualInterval-ms": "374.3700", "LudlController-Port": "COM9", "445 Shutter-Demo": "No", "PRIME-Trigger-Expose Out-Mux": "1", "SliceIndex": 9, "PRIME-CCDTemperatureSetPoint": "-15.0000", "405 Shutter-MinVolts": "0.0000", "PRIME-ExposeOutMode": "First Row", "AxisPositions": null, "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Volts": "0.0000", "528-Name": "DA Shutter", "555-State": "0", "XLIGHT Dichroic-Label": "Di03-R635-t1", "445 AO-Sequenceable": "1", "405-Name": "DA Shutter", "405 AO-IOChannel": "Dev1/ao0", "Core-Camera": "PRIME", "Focus-Name": "Focus", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "405 Shutter-IOChannel": "Dev1/ao8", "640 AO-Demo": "No", "640 Shutter-SupportsTriggering": "0", "445 AO-Demo": "No", "555-DA Device": "555 Shutter", "470 Shutter-Description": "NI DAC", "528 AO-Volts": "0.0000", "COM1-DataBits": "8", "Vincent-D3-Address": "x", "520 AO-SupportsTriggering": "0", "405-Description": "DA device that is used as a shutter", "640 Shutter-MinVolts": "0.0000", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "405 AO-Name": "AnalogIO", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "640 AO-TriggerInputLine": "/Dev1/PFI0", "405 AO-TriggerInputLine": "/Dev1/PFI0", "640-Description": "DA device that is used as a shutter", "445 Shutter-TriggerSampleRate": "100000", "470 Shutter-TriggerSequenceLength": "1000", "COM1-StopBits": "1", "520 Shutter-SupportsTriggering": "0", "405-State": "0", "Core-Initialize": "1", "XLIGHT Emission Wheel-State": "7", "520 AO-Description": "NI DAC", "528 AO-TriggerInputLine": "/Dev1/PFI0", "445 Shutter-MaxVolts": "5.0000", "COM9-Handshaking": "Off", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "XLIGHT Spin motor-Label": "On (spinning)", "PRIME-PP 5 ENABLED": "No", "UUID": "dc0e6044-b0b2-4f9b-9d26-9e5c72afdf71", "PRIME-TriggerMode": "Internal Trigger", "COM1-Handshaking": "Off", "PRIME-Timing-PostTriggerDelayNs": "49344000.0000", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "Focus-Position": "16479.8000", "528 Shutter-Block voltage": "false", "Channel": "P-DIC", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "555 Shutter-Demo": "No", "405 Shutter-Demo": "No", "528 AO-Name": "AnalogIO", "520-State": "0", "Core-Galvo": "", "405 Shutter-TriggerSampleRate": "100000", "Focus-FocusStepSize_(nm)": "100", "PRIME-PixelType": "16bit", "COM4-DataBits": "8", "470 AO-IOChannel": "Dev1/ao2", "520 AO-MinVolts": "0.0000", "COM9-Parity": "None", "PRIME-CircularBufferEnabled": "ON", "PRIME-PP 6 Reset": "No", "640 AO-Description": "NI DAC", "Source": "PRIME", "445 AO-MinVolts": "0.0000", "PIEZO-Name": "Stage", "445 AO-Description": "NI DAC", "PRIME-CircularBufferFrameCount": "46", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "520 AO-Volts": "0.0000", "528 Shutter-TriggerSampleRate": "100000", "XPositionUm": 62321, "OpticalPath-State": "4", "PRIME-PP 3 THRESHOLD": "80", "PRIME-PVCAM Version": "3.7.1", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Gain": "1-HDR", "640 Shutter-TriggerSequenceLength": "1000", "528 AO-SupportsTriggering": "0", "445 AO-TriggerInputLine": "/Dev1/PFI0", "OpticalPath-Name": "OpticalPath", "PRIME-PP 4 ENABLED": "Yes", "PRIME-PVCAM Adapter Version": "1.3.29", "COM3-Name": "COM3", "405 AO-MaxVolts": "5.0000", "520 Shutter-Block voltage": "false", "PRIME-Timing-ClearingTimeNs": "49344000.0000", "Core-Focus": "PIEZO", "528 AO-Block voltage": "false", "TE2000-Description": "Nikon TE2000 hub", "528 AO-MinVolts": "0.0000", "COM4-Parity": "None", "Summary": { "Slices": 11, "ChContrastMin": [ 0, 0, 0, 0, 0, 0 ], "PixelAspect": 1, "SlicesFirst": true, "Interval_ms": 0, "Time": "2019-12-17 21:58:39 -0500", "Prefix": "seq_3", "InitialPositionList": [ { "Label": "Pos0", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.435], "Focus": [16480.2], "XYStage": [ 69822.40000000001, 20100.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos1", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.425], "Focus": [16480.3], "XYStage": [ 69004, 20101 ] }, "GridColumnIndex": 0 }, { "Label": "Pos2", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.44], "Focus": [16480.1], "XYStage": [ 63119.5, 20690.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos3", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.405], "Focus": [16480.2], "XYStage": [ 62321, 20690.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos4", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.415], "Focus": [16480], "XYStage": [ 56604.100000000006, 20688.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos5", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.42000000000002], "Focus": [16480.1], "XYStage": [ 56034.200000000004, 20688.800000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos6", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.455], "Focus": [16480], "XYStage": [ 50889.3, 20496.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos7", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.43], "Focus": [16480.2], "XYStage": [ 50101.600000000006, 20496.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos8", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.67000000000002], "Focus": [16479.9], "XYStage": [ 44818.600000000006, 20496.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos9", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.67000000000002], "Focus": [16480], "XYStage": [ 43897.9, 20436.800000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos10", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.675], "Focus": [16479.9], "XYStage": [ 39670.600000000006, 20664.9 ] }, "GridColumnIndex": 0 }, { "Label": "Pos11", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.69], "Focus": [16479.9], "XYStage": [ 38734, 20664.600000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos12", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.695], "Focus": [16480.1], "XYStage": [ 34037.200000000004, 20664.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos13", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.685], "Focus": [16480.1], "XYStage": [ 33152, 20664.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos14", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.69], "Focus": [16480.1], "XYStage": [ 28122.800000000003, 20664.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos15", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.675], "Focus": [16480], "XYStage": [ 27422.9, 20664.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos16", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.64000000000001], "Focus": [16480], "XYStage": [ 23005.2, 20664.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos17", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.69], "Focus": [16479.9], "XYStage": [ 21820.600000000002, 20409.600000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos18", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.41], "Focus": [16480.1], "XYStage": [ 73697.6, -13222.800000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos19", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.455], "Focus": [16480.1], "XYStage": [ 72765.8, -13223 ] }, "GridColumnIndex": 0 }, { "Label": "Pos20", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.4], "Focus": [16480.2], "XYStage": [ 67445.5, -12303.800000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos21", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.415], "Focus": [16480.2], "XYStage": [ 66613.3, -12303.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos22", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.415], "Focus": [16480.1], "XYStage": [ 61819.4, -11936.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos23", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.405], "Focus": [16480.2], "XYStage": [ 61325.700000000004, -11937.1 ] }, "GridColumnIndex": 0 }, { "Label": "Pos24", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.46], "Focus": [16480.2], "XYStage": [ 55377, -10974.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos25", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.445], "Focus": [16480.1], "XYStage": [ 54827, -11214.800000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos26", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.435], "Focus": [16480.2], "XYStage": [ 49937.100000000006, -10919.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos27", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.425], "Focus": [16480.1], "XYStage": [ 48764, -10919.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos28", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.42000000000002], "Focus": [16480.2], "XYStage": [ 43978.4, -10920.400000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos29", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.435], "Focus": [16480.2], "XYStage": [ 43106.3, -10920.400000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos30", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.405], "Focus": [16480.3], "XYStage": [ 38212.4, -10920.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos31", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.445], "Focus": [16480.2], "XYStage": [ 37254.9, -10920.400000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos32", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.44], "Focus": [16480.3], "XYStage": [ 32472.800000000003, -11294.400000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos33", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [166.95000000000002], "Focus": [16480.2], "XYStage": [ 31420.7, -11294.400000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos34", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [166.95000000000002], "Focus": [16480.2], "XYStage": [ 26881.5, -11640 ] }, "GridColumnIndex": 0 }, { "Label": "Pos35", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [166.98], "Focus": [16480.2], "XYStage": [ 25876.100000000002, -11641.1 ] }, "GridColumnIndex": 0 } ], "z-step_um": 10, "GridRow": 0, "BitDepth": 16, "Depth": 2, "Channels": 6, "Source": "Micro-Manager", "ChColors": [ -16711681, -256, -65281, -1, -1, -16776961 ], "MetadataVersion": 10, "KeepShutterOpenChannels": true, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536, 65536 ], "CustomIntervals_ms": [], "Height": 1200, "MicroManagerVersion": "1.4.23 20180219", "GridColumn": 0, "UUID": "8c8f9d20-b128-4577-9f91-89e70f22d319", "PixelType": "GRAY16", "Width": 1200, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\Yan\\20191217-YC113-S5S6", "Frames": 1, "Comment": "", "UserName": "Nikon", "PixelSize_um": 1.1, "ROI": [ 0, 0, 1200, 1200 ], "Date": "2019-12-17", "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC", "P-DAPI-high" ], "KeepShutterOpenSlices": true, "IJType": 1, "ComputerName": "ZadorConfocal2", "Positions": 36, "PositionIndex": 3, "CameraTimeout": 20000, "TimeFirst": false }, "640 AO-Name": "AnalogIO", "Vincent-D3-Command": "Close" }, "FrameKey-0-4-10": { "COM4-StopBits": "1", "528 AO-MaxVolts": "5.0000", "XLIGHT Excitation Wheel-State": "2", "470 Shutter-Name": "AnalogIO", "Core-Shutter": "Vincent-D3", "555 AO-Demo": "No", "XLIGHT Dichroic-State": "2", "XYStage-StepSize-Y": "0.1000", "ZPositionUm": 227.405, "XYStage-StepSize-X": "0.1000", "PRIME-FirmwareVersion": "20.28", "PRIME-ClearCycles": "2", "520-Name": "DA Shutter", "405 Shutter-Name": "AnalogIO", "PIEZO-StepSize": "0.0050", "PRIME-ClearMode": "Pre-Exposure", "470 AO-MinVolts": "0.0000", "405 AO-SupportsTriggering": "1", "CameraChannelIndex": 0, "XYStage-StartSpeed": "500.0000", "COM9-StopBits": "2", "405 AO-Description": "NI DAC", "COM4-DelayBetweenCharsMs": "0.0000", "445 AO-Block voltage": "false", "520 AO-TriggerInputLine": "/Dev1/PFI0", "555 AO-Description": "NI DAC", "520 AO-TriggerSampleRate": "100000", "COM4-Verbose": "1", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM4-Name": "COM4", "405 Shutter-Volts": "0.0000", "Vincent-D3-Port": "COM1", "PRIME-PP 4 MIN ADU AFFECTED": "200", "470 Shutter-MinVolts": "0.0000", "PRIME-PP 3 ENABLED": "Yes", "470 AO-Description": "NI DAC", "PRIME-Description": "PVCAM API device adapter", "520 Shutter-MaxVolts": "5.0000", "520 Shutter-Volts": "0.0000", "528 AO-Sequenceable": "1", "PRIME-Binning": "1x1", "555 Shutter-MaxVolts": "5.0000", "528 Shutter-TriggerSequenceLength": "1000", "405 Shutter-Block voltage": "false", "XLIGHT Spin motor-State": "1", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "Vincent-D3-Time to open (ms)": "35.0000", "Vincent-D3-Time to close (ms)": "35.0000", "445 AO-TriggerSampleRate": "100000", "640 Shutter-IOChannel": "Dev1/ao14", "555 AO-Sequenceable": "1", "520 AO-Name": "AnalogIO", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "470 AO-Block voltage": "false", "COM1-Verbose": "1", "COM9-AnswerTimeout": "10000.0000", "528 Shutter-Name": "AnalogIO", "PRIME-Trigger Timeout (secs)": "10", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "NextFrame": 0, "470 AO-TriggerSequenceLength": "1000", "PRIME-TransposeXY": "0", "445 AO-TriggerSequenceLength": "1000", "COM1-Name": "COM1", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "COM9-DataBits": "8", "COM1-AnswerTimeout": "500.0000", "Camera": "", "TE2000-Version": "V2.50-V2.50-V2.10", "528 Shutter-Volts": "0.0000", "XLIGHT Hub-Name": "XLIGHT Hub", "PIEZO-Description": "Ludl stage driver adapter", "PositionName": "Pos3", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSampleRate": "100000", "PRIME-FanSpeedSetpoint": "High", "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "405 AO-TriggerSampleRate": "100000", "470 Shutter-Block voltage": "false", "470-State": "0", "COM3-Parity": "None", "405 AO-Volts": "0.0000", "PRIME-TransposeMirrorY": "0", "PRIME-ChipName": "GS144BSI", "470 AO-Volts": "0.0000", "PRIME-TransposeMirrorX": "0", "445 Shutter-SupportsTriggering": "0", "470 Shutter-SupportsTriggering": "0", "XLIGHT Hub-Description": "XLIGHT hub", "PRIME-PreampOffLimit": "10000", "470 AO-Sequenceable": "1", "528 Shutter-MaxVolts": "5.0000", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "Core-XYStage": "XYStage", "470 AO-TriggerSampleRate": "100000", "PRIME-Timing-PreTriggerDelayNs": "24672000.0000", "528-DA Device": "528 Shutter", "Focus-Description": "Nikon TE2000 focus adapter", "FilterBlock-State": "5", "520 AO-Demo": "No", "COM3-DelayBetweenCharsMs": "0.0000", "470 Shutter-Volts": "0.0000", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "PRIME-PP 2 THRESHOLD": "125", "PRIME-PP 4 THRESHOLD": "75", "XYStage-Acceleration": "4.0000", "470 Shutter-Demo": "No", "PRIME-Name": "PMPCIECam00", "640-DA Device": "640 Shutter", "405 AO-Block voltage": "false", "ElapsedTime-ms": 162538, "PRIME-Offset": "100", "445 AO-IOChannel": "Dev1/ao1", "640 AO-MaxVolts": "5.0000", "PositionIndex": 3, "PRIME-PP 1 ENABLED": "Yes", "COM1-BaudRate": "9600", "640-State": "0", "COM3-Verbose": "1", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "LudlController-TransmissionDelay": "0", "PRIME-FullWellCapacity": "62000", "PRIME-SMARTStreamingEnabled": "No", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "640 Shutter-Description": "NI DAC", "528-Description": "DA device that is used as a shutter", "COM4-Description": "Serial port driver (boost:asio)", "405 Shutter-Description": "NI DAC", "TE2000-Name": "TE2000", "528 Shutter-Description": "NI DAC", "COM9-Description": "Serial port driver (boost:asio)", "LudlController-Name": "LudlController", "445 Shutter-TriggerSequenceLength": "1000", "PRIME-SerialNumber": "A18B203008", "445 AO-Volts": "0.0000", "PRIME-X-dimension": "1200", "PRIME-ShutterCloseDelay": "0", "XLIGHT Disk slider-State": "2", "Position": "Default", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 AO-IOChannel": "Dev1/ao5", "520 AO-IOChannel": "Dev1/ao4", "528 AO-TriggerSequenceLength": "1000", "405 Shutter-MaxVolts": "5.0000", "470-DA Device": "470 Shutter", "PRIME-FTCapable": "No", "Core-ChannelGroup": "Channels", "555 AO-SupportsTriggering": "0", "PRIME-Y-dimension": "1200", "Frame": 0, "555 Shutter-Sequenceable": "1", "PRIME-Timing-ExposureTimeNs": "9992000.0000", "Core-ImageProcessor": "", "528-State": "0", "520 AO-Block voltage": "false", "XLIGHT Excitation Wheel-Label": "Excitation-2", "640 Shutter-Demo": "No", "COM3-StopBits": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "520 Shutter-IOChannel": "Dev1/ao11", "Width": 1200, "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-SupportsTriggering": "0", "640 AO-TriggerSampleRate": "100000", "520-Description": "DA device that is used as a shutter", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "405 Shutter-Sequenceable": "1", "405 Shutter-SupportsTriggering": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-TriggerSampleRate": "100000", "640 Shutter-MaxVolts": "5.0000", "555 AO-MaxVolts": "5.0000", "520 AO-TriggerSequenceLength": "1000", "COM3-BaudRate": "9600", "COM9-BaudRate": "9600", "Vincent-D3-Channel #": "Ch. #1", "PRIME-PMode": "Normal", "OpticalPath-Label": "Left(100)", "BitDepth": 16, "555 AO-TriggerSequenceLength": "1000", "445 Shutter-Description": "NI DAC", "405 AO-Sequenceable": "1", "PRIME-AcquisitionMethod": "Callbacks", "555 AO-Name": "AnalogIO", "PRIME-ShutterMode": "Pre-Exposure", "COM3-Handshaking": "Off", "445 AO-SupportsTriggering": "0", "ROI": "0-0-1200-1200", "COM9-Verbose": "1", "PRIME-Color": "OFF", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "PRIME-ShutterOpenDelay": "0", "COM1-Parity": "None", "Slice": 10, "SlicePosition": 50, "COM9-DelayBetweenCharsMs": "0.0000", "555-Name": "DA Shutter", "PIEZO-LudlSingleAxisName": "B", "XLIGHT Emission Wheel-Label": "Empty", "640 Shutter-Name": "AnalogIO", "640 AO-Sequenceable": "1", "520 Shutter-Demo": "No", "528 Shutter-Demo": "No", "470-Name": "DA Shutter", "520-DA Device": "520 Shutter", "XYStage-Description": "Ludl XY stage driver adapter", "445 Shutter-MinVolts": "0.0000", "528 AO-TriggerSampleRate": "100000", "405 AO-MinVolts": "0.0000", "405 Shutter-TriggerSequenceLength": "1000", "Height": 1200, "555 AO-Block voltage": "false", "640 Shutter-Volts": "0.0000", "PixelType": "GRAY16", "445 Shutter-Block voltage": "false", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "520 Shutter-TriggerSequenceLength": "1000", "COM9-Name": "COM9", "Core-SLM": "", "555 AO-Volts": "0.0000", "555 Shutter-Block voltage": "false", "XYStage-Speed": "1800.0000", "520 AO-MaxVolts": "5.0000", "528 Shutter-SupportsTriggering": "0", "COM1-Description": "Serial port driver (boost:asio)", "PRIME-MetadataEnabled": "No", "520 Shutter-MinVolts": "0.0000", "470 Shutter-Sequenceable": "1", "PRIME-PreampDelay": "0", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-MinVolts": "0.0000", "ChannelIndex": 4, "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Core-AutoShutter": "1", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "Exposure-ms": 20, "PRIME-PP 2 ENABLED": "Yes", "640 Shutter-TriggerSampleRate": "100000", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "470 Shutter-IOChannel": "Dev1/ao10", "405 AO-Demo": "No", "PixelSizeUm": 1.1, "470 AO-Demo": "No", "470 Shutter-MaxVolts": "5.0000", "Vincent-D3-Name": "Vincent-D3", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FileName": "img_000000000_P-DIC_010.tif", "LudlController-Reset": "Operate", "PRIME-Exposure": "20.00", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "445-Name": "DA Shutter", "555 Shutter-Name": "AnalogIO", "520 Shutter-TriggerSampleRate": "100000", "445 Shutter-IOChannel": "Dev1/ao9", "PRIME-TransposeCorrection": "0", "XYStage-StepSize": "0.1000", "445-Description": "DA device that is used as a shutter", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 AO-IOChannel": "Dev1/ao7", "520 Shutter-Name": "AnalogIO", "COM3-DataBits": "8", "640 AO-Volts": "0.0000", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "555 Shutter-IOChannel": "Dev1/ao13", "WaitInterval": null, "520 Shutter-Sequenceable": "1", "YPositionUm": 20690.300000000003, "445-State": "0", "445-DA Device": "445 Shutter", "PRIME-CircularBufferAutoSize": "ON", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "555 AO-TriggerSampleRate": "100000", "445 Shutter-Volts": "0.0000", "XLIGHT Hub-Port": "COM3", "Core-AutoFocus": "", "COM4-Handshaking": "Off", "528 Shutter-MinVolts": "0.0000", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "445 Shutter-Name": "AnalogIO", "528 Shutter-Sequenceable": "1", "XYStage-TransposeMirrorX": "0", "XYStage-TransposeMirrorY": "0", "PRIME-ReadoutRate": "100MHz 16bit", "XYStage-Name": "Stage", "640 AO-SupportsTriggering": "0", "640-Name": "DA Shutter", "640 Shutter-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "640 AO-Block voltage": "false", "445 Shutter-Sequenceable": "1", "470 AO-MaxVolts": "5.0000", "COM4-BaudRate": "9600", "470-Description": "DA device that is used as a shutter", "520 Shutter-Description": "NI DAC", "PRIME-CCDTemperature": "-14.9600", "Time": "2019-12-17 21:59:10 -0500", "640 AO-TriggerSequenceLength": "1000", "470 AO-Name": "AnalogIO", "XLIGHT Disk slider-Label": "Disk Pos 40um", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "Binning": "1x1", "TE2000-ModelType": "1", "COM3-AnswerTimeout": "500.0000", "555 Shutter-SupportsTriggering": "0", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "TE2000-Port": "COM4", "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Description": "NI DAC", "COM4-AnswerTimeout": "500.0000", "COM1-DelayBetweenCharsMs": "0.0000", "FilterBlock-Label": "Confocal", "445 AO-Name": "AnalogIO", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-Timing-ReadoutTimeNs": "24672000.0000", "528 AO-Demo": "No", "520 AO-Sequenceable": "1", "555-Description": "DA device that is used as a shutter", "405-DA Device": "405 Shutter", "405 AO-TriggerSequenceLength": "1000", "640 AO-MinVolts": "0.0000", "640 Shutter-Block voltage": "false", "FrameIndex": 0, "PRIME-Port": "CMOS", "555 AO-MinVolts": "0.0000", "COM3-Description": "Serial port driver (boost:asio)", "528 AO-Description": "NI DAC", "555 AO-IOChannel": "Dev1/ao6", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "555 AO-TriggerInputLine": "/Dev1/PFI0", "PRIME-ActualInterval-ms": "374.3700", "LudlController-Port": "COM9", "445 Shutter-Demo": "No", "PRIME-Trigger-Expose Out-Mux": "1", "SliceIndex": 10, "PRIME-CCDTemperatureSetPoint": "-15.0000", "405 Shutter-MinVolts": "0.0000", "PRIME-ExposeOutMode": "First Row", "AxisPositions": null, "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Volts": "0.0000", "528-Name": "DA Shutter", "555-State": "0", "XLIGHT Dichroic-Label": "Di03-R635-t1", "445 AO-Sequenceable": "1", "405-Name": "DA Shutter", "405 AO-IOChannel": "Dev1/ao0", "Core-Camera": "PRIME", "Focus-Name": "Focus", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "405 Shutter-IOChannel": "Dev1/ao8", "640 AO-Demo": "No", "640 Shutter-SupportsTriggering": "0", "445 AO-Demo": "No", "555-DA Device": "555 Shutter", "470 Shutter-Description": "NI DAC", "528 AO-Volts": "0.0000", "COM1-DataBits": "8", "Vincent-D3-Address": "x", "520 AO-SupportsTriggering": "0", "405-Description": "DA device that is used as a shutter", "640 Shutter-MinVolts": "0.0000", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "405 AO-Name": "AnalogIO", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "640 AO-TriggerInputLine": "/Dev1/PFI0", "405 AO-TriggerInputLine": "/Dev1/PFI0", "640-Description": "DA device that is used as a shutter", "445 Shutter-TriggerSampleRate": "100000", "470 Shutter-TriggerSequenceLength": "1000", "COM1-StopBits": "1", "520 Shutter-SupportsTriggering": "0", "405-State": "0", "Core-Initialize": "1", "XLIGHT Emission Wheel-State": "7", "520 AO-Description": "NI DAC", "528 AO-TriggerInputLine": "/Dev1/PFI0", "445 Shutter-MaxVolts": "5.0000", "COM9-Handshaking": "Off", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "XLIGHT Spin motor-Label": "On (spinning)", "PRIME-PP 5 ENABLED": "No", "UUID": "c4f9a87d-d8a5-4694-bc51-aa8970b1ba59", "PRIME-TriggerMode": "Internal Trigger", "COM1-Handshaking": "Off", "PRIME-Timing-PostTriggerDelayNs": "49344000.0000", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "Focus-Position": "16479.8000", "528 Shutter-Block voltage": "false", "Channel": "P-DIC", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "555 Shutter-Demo": "No", "405 Shutter-Demo": "No", "528 AO-Name": "AnalogIO", "520-State": "0", "Core-Galvo": "", "405 Shutter-TriggerSampleRate": "100000", "Focus-FocusStepSize_(nm)": "100", "PRIME-PixelType": "16bit", "COM4-DataBits": "8", "470 AO-IOChannel": "Dev1/ao2", "520 AO-MinVolts": "0.0000", "COM9-Parity": "None", "PRIME-CircularBufferEnabled": "ON", "PRIME-PP 6 Reset": "No", "640 AO-Description": "NI DAC", "Source": "PRIME", "445 AO-MinVolts": "0.0000", "PIEZO-Name": "Stage", "445 AO-Description": "NI DAC", "PRIME-CircularBufferFrameCount": "46", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "520 AO-Volts": "0.0000", "528 Shutter-TriggerSampleRate": "100000", "XPositionUm": 62321, "OpticalPath-State": "4", "PRIME-PP 3 THRESHOLD": "80", "PRIME-PVCAM Version": "3.7.1", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Gain": "1-HDR", "640 Shutter-TriggerSequenceLength": "1000", "528 AO-SupportsTriggering": "0", "445 AO-TriggerInputLine": "/Dev1/PFI0", "OpticalPath-Name": "OpticalPath", "PRIME-PP 4 ENABLED": "Yes", "PRIME-PVCAM Adapter Version": "1.3.29", "COM3-Name": "COM3", "405 AO-MaxVolts": "5.0000", "520 Shutter-Block voltage": "false", "PRIME-Timing-ClearingTimeNs": "49344000.0000", "Core-Focus": "PIEZO", "528 AO-Block voltage": "false", "TE2000-Description": "Nikon TE2000 hub", "528 AO-MinVolts": "0.0000", "COM4-Parity": "None", "Summary": { "Slices": 11, "ChContrastMin": [ 0, 0, 0, 0, 0, 0 ], "PixelAspect": 1, "SlicesFirst": true, "Interval_ms": 0, "Time": "2019-12-17 21:58:39 -0500", "Prefix": "seq_3", "InitialPositionList": [ { "Label": "Pos0", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.435], "Focus": [16480.2], "XYStage": [ 69822.40000000001, 20100.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos1", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.425], "Focus": [16480.3], "XYStage": [ 69004, 20101 ] }, "GridColumnIndex": 0 }, { "Label": "Pos2", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.44], "Focus": [16480.1], "XYStage": [ 63119.5, 20690.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos3", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.405], "Focus": [16480.2], "XYStage": [ 62321, 20690.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos4", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.415], "Focus": [16480], "XYStage": [ 56604.100000000006, 20688.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos5", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.42000000000002], "Focus": [16480.1], "XYStage": [ 56034.200000000004, 20688.800000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos6", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.455], "Focus": [16480], "XYStage": [ 50889.3, 20496.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos7", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.43], "Focus": [16480.2], "XYStage": [ 50101.600000000006, 20496.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos8", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.67000000000002], "Focus": [16479.9], "XYStage": [ 44818.600000000006, 20496.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos9", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.67000000000002], "Focus": [16480], "XYStage": [ 43897.9, 20436.800000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos10", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.675], "Focus": [16479.9], "XYStage": [ 39670.600000000006, 20664.9 ] }, "GridColumnIndex": 0 }, { "Label": "Pos11", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.69], "Focus": [16479.9], "XYStage": [ 38734, 20664.600000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos12", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.695], "Focus": [16480.1], "XYStage": [ 34037.200000000004, 20664.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos13", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.685], "Focus": [16480.1], "XYStage": [ 33152, 20664.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos14", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.69], "Focus": [16480.1], "XYStage": [ 28122.800000000003, 20664.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos15", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.675], "Focus": [16480], "XYStage": [ 27422.9, 20664.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos16", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.64000000000001], "Focus": [16480], "XYStage": [ 23005.2, 20664.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos17", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.69], "Focus": [16479.9], "XYStage": [ 21820.600000000002, 20409.600000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos18", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.41], "Focus": [16480.1], "XYStage": [ 73697.6, -13222.800000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos19", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.455], "Focus": [16480.1], "XYStage": [ 72765.8, -13223 ] }, "GridColumnIndex": 0 }, { "Label": "Pos20", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.4], "Focus": [16480.2], "XYStage": [ 67445.5, -12303.800000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos21", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.415], "Focus": [16480.2], "XYStage": [ 66613.3, -12303.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos22", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.415], "Focus": [16480.1], "XYStage": [ 61819.4, -11936.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos23", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.405], "Focus": [16480.2], "XYStage": [ 61325.700000000004, -11937.1 ] }, "GridColumnIndex": 0 }, { "Label": "Pos24", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.46], "Focus": [16480.2], "XYStage": [ 55377, -10974.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos25", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.445], "Focus": [16480.1], "XYStage": [ 54827, -11214.800000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos26", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.435], "Focus": [16480.2], "XYStage": [ 49937.100000000006, -10919.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos27", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.425], "Focus": [16480.1], "XYStage": [ 48764, -10919.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos28", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.42000000000002], "Focus": [16480.2], "XYStage": [ 43978.4, -10920.400000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos29", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.435], "Focus": [16480.2], "XYStage": [ 43106.3, -10920.400000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos30", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.405], "Focus": [16480.3], "XYStage": [ 38212.4, -10920.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos31", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.445], "Focus": [16480.2], "XYStage": [ 37254.9, -10920.400000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos32", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.44], "Focus": [16480.3], "XYStage": [ 32472.800000000003, -11294.400000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos33", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [166.95000000000002], "Focus": [16480.2], "XYStage": [ 31420.7, -11294.400000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos34", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [166.95000000000002], "Focus": [16480.2], "XYStage": [ 26881.5, -11640 ] }, "GridColumnIndex": 0 }, { "Label": "Pos35", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [166.98], "Focus": [16480.2], "XYStage": [ 25876.100000000002, -11641.1 ] }, "GridColumnIndex": 0 } ], "z-step_um": 10, "GridRow": 0, "BitDepth": 16, "Depth": 2, "Channels": 6, "Source": "Micro-Manager", "ChColors": [ -16711681, -256, -65281, -1, -1, -16776961 ], "MetadataVersion": 10, "KeepShutterOpenChannels": true, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536, 65536 ], "CustomIntervals_ms": [], "Height": 1200, "MicroManagerVersion": "1.4.23 20180219", "GridColumn": 0, "UUID": "8c8f9d20-b128-4577-9f91-89e70f22d319", "PixelType": "GRAY16", "Width": 1200, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\Yan\\20191217-YC113-S5S6", "Frames": 1, "Comment": "", "UserName": "Nikon", "PixelSize_um": 1.1, "ROI": [ 0, 0, 1200, 1200 ], "Date": "2019-12-17", "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC", "P-DAPI-high" ], "KeepShutterOpenSlices": true, "IJType": 1, "ComputerName": "ZadorConfocal2", "Positions": 36, "PositionIndex": 3, "CameraTimeout": 20000, "TimeFirst": false }, "640 AO-Name": "AnalogIO", "Vincent-D3-Command": "Close" }, "FrameKey-0-5-0": { "COM4-StopBits": "1", "528 AO-MaxVolts": "5.0000", "XLIGHT Excitation Wheel-State": "2", "470 Shutter-Name": "AnalogIO", "Core-Shutter": "405", "555 AO-Demo": "No", "XLIGHT Dichroic-State": "2", "XYStage-StepSize-Y": "0.1000", "ZPositionUm": 127.405, "XYStage-StepSize-X": "0.1000", "PRIME-FirmwareVersion": "20.28", "PRIME-ClearCycles": "2", "520-Name": "DA Shutter", "405 Shutter-Name": "AnalogIO", "PIEZO-StepSize": "0.0050", "PRIME-ClearMode": "Pre-Exposure", "470 AO-MinVolts": "0.0000", "405 AO-SupportsTriggering": "1", "CameraChannelIndex": 0, "XYStage-StartSpeed": "500.0000", "COM9-StopBits": "2", "405 AO-Description": "NI DAC", "COM4-DelayBetweenCharsMs": "0.0000", "445 AO-Block voltage": "false", "520 AO-TriggerInputLine": "/Dev1/PFI0", "555 AO-Description": "NI DAC", "520 AO-TriggerSampleRate": "100000", "COM4-Verbose": "1", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM4-Name": "COM4", "405 Shutter-Volts": "5.0000", "Vincent-D3-Port": "COM1", "PRIME-PP 4 MIN ADU AFFECTED": "200", "470 Shutter-MinVolts": "0.0000", "PRIME-PP 3 ENABLED": "Yes", "470 AO-Description": "NI DAC", "PRIME-Description": "PVCAM API device adapter", "520 Shutter-MaxVolts": "5.0000", "520 Shutter-Volts": "0.0000", "528 AO-Sequenceable": "1", "PRIME-Binning": "1x1", "555 Shutter-MaxVolts": "5.0000", "528 Shutter-TriggerSequenceLength": "1000", "405 Shutter-Block voltage": "false", "XLIGHT Spin motor-State": "1", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "Vincent-D3-Time to open (ms)": "35.0000", "Vincent-D3-Time to close (ms)": "35.0000", "445 AO-TriggerSampleRate": "100000", "640 Shutter-IOChannel": "Dev1/ao14", "555 AO-Sequenceable": "1", "520 AO-Name": "AnalogIO", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "470 AO-Block voltage": "false", "COM1-Verbose": "1", "COM9-AnswerTimeout": "10000.0000", "528 Shutter-Name": "AnalogIO", "PRIME-Trigger Timeout (secs)": "10", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "NextFrame": 0, "470 AO-TriggerSequenceLength": "1000", "PRIME-TransposeXY": "0", "445 AO-TriggerSequenceLength": "1000", "COM1-Name": "COM1", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "COM9-DataBits": "8", "COM1-AnswerTimeout": "500.0000", "Camera": "", "TE2000-Version": "V2.50-V2.50-V2.10", "528 Shutter-Volts": "0.0000", "XLIGHT Hub-Name": "XLIGHT Hub", "PIEZO-Description": "Ludl stage driver adapter", "PositionName": "Pos3", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSampleRate": "100000", "PRIME-FanSpeedSetpoint": "High", "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "405 AO-TriggerSampleRate": "100000", "470 Shutter-Block voltage": "false", "470-State": "0", "COM3-Parity": "None", "405 AO-Volts": "4.5000", "PRIME-TransposeMirrorY": "0", "PRIME-ChipName": "GS144BSI", "470 AO-Volts": "0.0000", "PRIME-TransposeMirrorX": "0", "445 Shutter-SupportsTriggering": "0", "470 Shutter-SupportsTriggering": "0", "XLIGHT Hub-Description": "XLIGHT hub", "PRIME-PreampOffLimit": "10000", "470 AO-Sequenceable": "1", "528 Shutter-MaxVolts": "5.0000", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "Core-XYStage": "XYStage", "470 AO-TriggerSampleRate": "100000", "PRIME-Timing-PreTriggerDelayNs": "24672000.0000", "528-DA Device": "528 Shutter", "Focus-Description": "Nikon TE2000 focus adapter", "FilterBlock-State": "5", "520 AO-Demo": "No", "COM3-DelayBetweenCharsMs": "0.0000", "470 Shutter-Volts": "0.0000", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "PRIME-PP 2 THRESHOLD": "125", "PRIME-PP 4 THRESHOLD": "75", "XYStage-Acceleration": "4.0000", "470 Shutter-Demo": "No", "PRIME-Name": "PMPCIECam00", "640-DA Device": "640 Shutter", "405 AO-Block voltage": "false", "ElapsedTime-ms": 163880, "PRIME-Offset": "100", "445 AO-IOChannel": "Dev1/ao1", "640 AO-MaxVolts": "5.0000", "PositionIndex": 3, "PRIME-PP 1 ENABLED": "Yes", "COM1-BaudRate": "9600", "640-State": "0", "COM3-Verbose": "1", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "LudlController-TransmissionDelay": "0", "PRIME-FullWellCapacity": "62000", "PRIME-SMARTStreamingEnabled": "No", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "640 Shutter-Description": "NI DAC", "528-Description": "DA device that is used as a shutter", "COM4-Description": "Serial port driver (boost:asio)", "405 Shutter-Description": "NI DAC", "TE2000-Name": "TE2000", "528 Shutter-Description": "NI DAC", "COM9-Description": "Serial port driver (boost:asio)", "LudlController-Name": "LudlController", "445 Shutter-TriggerSequenceLength": "1000", "PRIME-SerialNumber": "A18B203008", "445 AO-Volts": "0.0000", "PRIME-X-dimension": "1200", "PRIME-ShutterCloseDelay": "0", "XLIGHT Disk slider-State": "2", "Position": "Default", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 AO-IOChannel": "Dev1/ao5", "520 AO-IOChannel": "Dev1/ao4", "528 AO-TriggerSequenceLength": "1000", "405 Shutter-MaxVolts": "5.0000", "470-DA Device": "470 Shutter", "PRIME-FTCapable": "No", "Core-ChannelGroup": "Channels", "555 AO-SupportsTriggering": "0", "PRIME-Y-dimension": "1200", "Frame": 0, "555 Shutter-Sequenceable": "1", "PRIME-Timing-ExposureTimeNs": "9992000.0000", "Core-ImageProcessor": "", "528-State": "0", "520 AO-Block voltage": "false", "XLIGHT Excitation Wheel-Label": "QUAD", "640 Shutter-Demo": "No", "COM3-StopBits": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "520 Shutter-IOChannel": "Dev1/ao11", "Width": 1200, "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-SupportsTriggering": "0", "640 AO-TriggerSampleRate": "100000", "520-Description": "DA device that is used as a shutter", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "405 Shutter-Sequenceable": "1", "405 Shutter-SupportsTriggering": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-TriggerSampleRate": "100000", "640 Shutter-MaxVolts": "5.0000", "555 AO-MaxVolts": "5.0000", "520 AO-TriggerSequenceLength": "1000", "COM3-BaudRate": "9600", "COM9-BaudRate": "9600", "Vincent-D3-Channel #": "Ch. #1", "PRIME-PMode": "Normal", "OpticalPath-Label": "Left(100)", "BitDepth": 16, "555 AO-TriggerSequenceLength": "1000", "445 Shutter-Description": "NI DAC", "405 AO-Sequenceable": "1", "PRIME-AcquisitionMethod": "Callbacks", "555 AO-Name": "AnalogIO", "PRIME-ShutterMode": "Pre-Exposure", "COM3-Handshaking": "Off", "445 AO-SupportsTriggering": "0", "ROI": "0-0-1200-1200", "COM9-Verbose": "1", "PRIME-Color": "OFF", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "PRIME-ShutterOpenDelay": "0", "COM1-Parity": "None", "Slice": 0, "SlicePosition": -50, "COM9-DelayBetweenCharsMs": "0.0000", "555-Name": "DA Shutter", "PIEZO-LudlSingleAxisName": "B", "XLIGHT Emission Wheel-Label": "QUAD", "640 Shutter-Name": "AnalogIO", "640 AO-Sequenceable": "1", "520 Shutter-Demo": "No", "528 Shutter-Demo": "No", "470-Name": "DA Shutter", "520-DA Device": "520 Shutter", "XYStage-Description": "Ludl XY stage driver adapter", "445 Shutter-MinVolts": "0.0000", "528 AO-TriggerSampleRate": "100000", "405 AO-MinVolts": "0.0000", "405 Shutter-TriggerSequenceLength": "1000", "Height": 1200, "555 AO-Block voltage": "false", "640 Shutter-Volts": "0.0000", "PixelType": "GRAY16", "445 Shutter-Block voltage": "false", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "520 Shutter-TriggerSequenceLength": "1000", "COM9-Name": "COM9", "Core-SLM": "", "555 AO-Volts": "0.0000", "555 Shutter-Block voltage": "false", "XYStage-Speed": "1800.0000", "520 AO-MaxVolts": "5.0000", "528 Shutter-SupportsTriggering": "0", "COM1-Description": "Serial port driver (boost:asio)", "PRIME-MetadataEnabled": "No", "520 Shutter-MinVolts": "0.0000", "470 Shutter-Sequenceable": "1", "PRIME-PreampDelay": "0", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-MinVolts": "0.0000", "ChannelIndex": 5, "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Core-AutoShutter": "1", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "Exposure-ms": 300, "PRIME-PP 2 ENABLED": "Yes", "640 Shutter-TriggerSampleRate": "100000", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "470 Shutter-IOChannel": "Dev1/ao10", "405 AO-Demo": "No", "PixelSizeUm": 1.1, "470 AO-Demo": "No", "470 Shutter-MaxVolts": "5.0000", "Vincent-D3-Name": "Vincent-D3", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FileName": "img_000000000_P-DAPI-high_000.tif", "LudlController-Reset": "Operate", "PRIME-Exposure": "300.00", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "445-Name": "DA Shutter", "555 Shutter-Name": "AnalogIO", "520 Shutter-TriggerSampleRate": "100000", "445 Shutter-IOChannel": "Dev1/ao9", "PRIME-TransposeCorrection": "0", "XYStage-StepSize": "0.1000", "445-Description": "DA device that is used as a shutter", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 AO-IOChannel": "Dev1/ao7", "520 Shutter-Name": "AnalogIO", "COM3-DataBits": "8", "640 AO-Volts": "0.0000", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "555 Shutter-IOChannel": "Dev1/ao13", "WaitInterval": null, "520 Shutter-Sequenceable": "1", "YPositionUm": 20690.300000000003, "445-State": "0", "445-DA Device": "445 Shutter", "PRIME-CircularBufferAutoSize": "ON", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "555 AO-TriggerSampleRate": "100000", "445 Shutter-Volts": "0.0000", "XLIGHT Hub-Port": "COM3", "Core-AutoFocus": "", "COM4-Handshaking": "Off", "528 Shutter-MinVolts": "0.0000", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "445 Shutter-Name": "AnalogIO", "528 Shutter-Sequenceable": "1", "XYStage-TransposeMirrorX": "0", "XYStage-TransposeMirrorY": "0", "PRIME-ReadoutRate": "100MHz 16bit", "XYStage-Name": "Stage", "640 AO-SupportsTriggering": "0", "640-Name": "DA Shutter", "640 Shutter-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "640 AO-Block voltage": "false", "445 Shutter-Sequenceable": "1", "470 AO-MaxVolts": "5.0000", "COM4-BaudRate": "9600", "470-Description": "DA device that is used as a shutter", "520 Shutter-Description": "NI DAC", "PRIME-CCDTemperature": "-14.9600", "Time": "2019-12-17 21:59:11 -0500", "640 AO-TriggerSequenceLength": "1000", "470 AO-Name": "AnalogIO", "XLIGHT Disk slider-Label": "Disk Pos 40um", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "Binning": "1x1", "TE2000-ModelType": "1", "COM3-AnswerTimeout": "500.0000", "555 Shutter-SupportsTriggering": "0", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "TE2000-Port": "COM4", "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Description": "NI DAC", "COM4-AnswerTimeout": "500.0000", "COM1-DelayBetweenCharsMs": "0.0000", "FilterBlock-Label": "Confocal", "445 AO-Name": "AnalogIO", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-Timing-ReadoutTimeNs": "24672000.0000", "528 AO-Demo": "No", "520 AO-Sequenceable": "1", "555-Description": "DA device that is used as a shutter", "405-DA Device": "405 Shutter", "405 AO-TriggerSequenceLength": "1000", "640 AO-MinVolts": "0.0000", "640 Shutter-Block voltage": "false", "FrameIndex": 0, "PRIME-Port": "CMOS", "555 AO-MinVolts": "0.0000", "COM3-Description": "Serial port driver (boost:asio)", "528 AO-Description": "NI DAC", "555 AO-IOChannel": "Dev1/ao6", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "555 AO-TriggerInputLine": "/Dev1/PFI0", "PRIME-ActualInterval-ms": "374.3700", "LudlController-Port": "COM9", "445 Shutter-Demo": "No", "PRIME-Trigger-Expose Out-Mux": "1", "SliceIndex": 0, "PRIME-CCDTemperatureSetPoint": "-15.0000", "405 Shutter-MinVolts": "0.0000", "PRIME-ExposeOutMode": "First Row", "AxisPositions": null, "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Volts": "0.0000", "528-Name": "DA Shutter", "555-State": "0", "XLIGHT Dichroic-Label": "QUAD", "445 AO-Sequenceable": "1", "405-Name": "DA Shutter", "405 AO-IOChannel": "Dev1/ao0", "Core-Camera": "PRIME", "Focus-Name": "Focus", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "405 Shutter-IOChannel": "Dev1/ao8", "640 AO-Demo": "No", "640 Shutter-SupportsTriggering": "0", "445 AO-Demo": "No", "555-DA Device": "555 Shutter", "470 Shutter-Description": "NI DAC", "528 AO-Volts": "0.0000", "COM1-DataBits": "8", "Vincent-D3-Address": "x", "520 AO-SupportsTriggering": "0", "405-Description": "DA device that is used as a shutter", "640 Shutter-MinVolts": "0.0000", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "405 AO-Name": "AnalogIO", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "640 AO-TriggerInputLine": "/Dev1/PFI0", "405 AO-TriggerInputLine": "/Dev1/PFI0", "640-Description": "DA device that is used as a shutter", "445 Shutter-TriggerSampleRate": "100000", "470 Shutter-TriggerSequenceLength": "1000", "COM1-StopBits": "1", "520 Shutter-SupportsTriggering": "0", "405-State": "1", "Core-Initialize": "1", "XLIGHT Emission Wheel-State": "7", "520 AO-Description": "NI DAC", "528 AO-TriggerInputLine": "/Dev1/PFI0", "445 Shutter-MaxVolts": "5.0000", "COM9-Handshaking": "Off", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "XLIGHT Spin motor-Label": "On (spinning)", "PRIME-PP 5 ENABLED": "No", "UUID": "428efbd8-6405-4620-9ca4-10d9db3446f0", "PRIME-TriggerMode": "Internal Trigger", "COM1-Handshaking": "Off", "PRIME-Timing-PostTriggerDelayNs": "49344000.0000", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "Focus-Position": "16479.8000", "528 Shutter-Block voltage": "false", "Channel": "P-DAPI-high", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "555 Shutter-Demo": "No", "405 Shutter-Demo": "No", "528 AO-Name": "AnalogIO", "520-State": "0", "Core-Galvo": "", "405 Shutter-TriggerSampleRate": "100000", "Focus-FocusStepSize_(nm)": "100", "PRIME-PixelType": "16bit", "COM4-DataBits": "8", "470 AO-IOChannel": "Dev1/ao2", "520 AO-MinVolts": "0.0000", "COM9-Parity": "None", "PRIME-CircularBufferEnabled": "ON", "PRIME-PP 6 Reset": "No", "640 AO-Description": "NI DAC", "Source": "PRIME", "445 AO-MinVolts": "0.0000", "PIEZO-Name": "Stage", "445 AO-Description": "NI DAC", "PRIME-CircularBufferFrameCount": "46", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "520 AO-Volts": "0.0000", "528 Shutter-TriggerSampleRate": "100000", "XPositionUm": 62321, "OpticalPath-State": "4", "PRIME-PP 3 THRESHOLD": "80", "PRIME-PVCAM Version": "3.7.1", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Gain": "1-HDR", "640 Shutter-TriggerSequenceLength": "1000", "528 AO-SupportsTriggering": "0", "445 AO-TriggerInputLine": "/Dev1/PFI0", "OpticalPath-Name": "OpticalPath", "PRIME-PP 4 ENABLED": "Yes", "PRIME-PVCAM Adapter Version": "1.3.29", "COM3-Name": "COM3", "405 AO-MaxVolts": "5.0000", "520 Shutter-Block voltage": "false", "PRIME-Timing-ClearingTimeNs": "49344000.0000", "Core-Focus": "PIEZO", "528 AO-Block voltage": "false", "TE2000-Description": "Nikon TE2000 hub", "528 AO-MinVolts": "0.0000", "COM4-Parity": "None", "Summary": { "Slices": 11, "ChContrastMin": [ 0, 0, 0, 0, 0, 0 ], "PixelAspect": 1, "SlicesFirst": true, "Interval_ms": 0, "Time": "2019-12-17 21:58:39 -0500", "Prefix": "seq_3", "InitialPositionList": [ { "Label": "Pos0", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.435], "Focus": [16480.2], "XYStage": [ 69822.40000000001, 20100.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos1", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.425], "Focus": [16480.3], "XYStage": [ 69004, 20101 ] }, "GridColumnIndex": 0 }, { "Label": "Pos2", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.44], "Focus": [16480.1], "XYStage": [ 63119.5, 20690.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos3", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.405], "Focus": [16480.2], "XYStage": [ 62321, 20690.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos4", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.415], "Focus": [16480], "XYStage": [ 56604.100000000006, 20688.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos5", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.42000000000002], "Focus": [16480.1], "XYStage": [ 56034.200000000004, 20688.800000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos6", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.455], "Focus": [16480], "XYStage": [ 50889.3, 20496.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos7", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.43], "Focus": [16480.2], "XYStage": [ 50101.600000000006, 20496.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos8", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.67000000000002], "Focus": [16479.9], "XYStage": [ 44818.600000000006, 20496.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos9", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.67000000000002], "Focus": [16480], "XYStage": [ 43897.9, 20436.800000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos10", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.675], "Focus": [16479.9], "XYStage": [ 39670.600000000006, 20664.9 ] }, "GridColumnIndex": 0 }, { "Label": "Pos11", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.69], "Focus": [16479.9], "XYStage": [ 38734, 20664.600000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos12", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.695], "Focus": [16480.1], "XYStage": [ 34037.200000000004, 20664.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos13", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.685], "Focus": [16480.1], "XYStage": [ 33152, 20664.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos14", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.69], "Focus": [16480.1], "XYStage": [ 28122.800000000003, 20664.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos15", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.675], "Focus": [16480], "XYStage": [ 27422.9, 20664.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos16", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.64000000000001], "Focus": [16480], "XYStage": [ 23005.2, 20664.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos17", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.69], "Focus": [16479.9], "XYStage": [ 21820.600000000002, 20409.600000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos18", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.41], "Focus": [16480.1], "XYStage": [ 73697.6, -13222.800000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos19", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.455], "Focus": [16480.1], "XYStage": [ 72765.8, -13223 ] }, "GridColumnIndex": 0 }, { "Label": "Pos20", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.4], "Focus": [16480.2], "XYStage": [ 67445.5, -12303.800000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos21", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.415], "Focus": [16480.2], "XYStage": [ 66613.3, -12303.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos22", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.415], "Focus": [16480.1], "XYStage": [ 61819.4, -11936.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos23", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.405], "Focus": [16480.2], "XYStage": [ 61325.700000000004, -11937.1 ] }, "GridColumnIndex": 0 }, { "Label": "Pos24", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.46], "Focus": [16480.2], "XYStage": [ 55377, -10974.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos25", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.445], "Focus": [16480.1], "XYStage": [ 54827, -11214.800000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos26", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.435], "Focus": [16480.2], "XYStage": [ 49937.100000000006, -10919.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos27", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.425], "Focus": [16480.1], "XYStage": [ 48764, -10919.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos28", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.42000000000002], "Focus": [16480.2], "XYStage": [ 43978.4, -10920.400000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos29", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.435], "Focus": [16480.2], "XYStage": [ 43106.3, -10920.400000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos30", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.405], "Focus": [16480.3], "XYStage": [ 38212.4, -10920.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos31", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.445], "Focus": [16480.2], "XYStage": [ 37254.9, -10920.400000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos32", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.44], "Focus": [16480.3], "XYStage": [ 32472.800000000003, -11294.400000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos33", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [166.95000000000002], "Focus": [16480.2], "XYStage": [ 31420.7, -11294.400000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos34", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [166.95000000000002], "Focus": [16480.2], "XYStage": [ 26881.5, -11640 ] }, "GridColumnIndex": 0 }, { "Label": "Pos35", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [166.98], "Focus": [16480.2], "XYStage": [ 25876.100000000002, -11641.1 ] }, "GridColumnIndex": 0 } ], "z-step_um": 10, "GridRow": 0, "BitDepth": 16, "Depth": 2, "Channels": 6, "Source": "Micro-Manager", "ChColors": [ -16711681, -256, -65281, -1, -1, -16776961 ], "MetadataVersion": 10, "KeepShutterOpenChannels": true, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536, 65536 ], "CustomIntervals_ms": [], "Height": 1200, "MicroManagerVersion": "1.4.23 20180219", "GridColumn": 0, "UUID": "8c8f9d20-b128-4577-9f91-89e70f22d319", "PixelType": "GRAY16", "Width": 1200, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\Yan\\20191217-YC113-S5S6", "Frames": 1, "Comment": "", "UserName": "Nikon", "PixelSize_um": 1.1, "ROI": [ 0, 0, 1200, 1200 ], "Date": "2019-12-17", "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC", "P-DAPI-high" ], "KeepShutterOpenSlices": true, "IJType": 1, "ComputerName": "ZadorConfocal2", "Positions": 36, "PositionIndex": 3, "CameraTimeout": 20000, "TimeFirst": false }, "640 AO-Name": "AnalogIO", "Vincent-D3-Command": "Close" }, "FrameKey-0-5-1": { "COM4-StopBits": "1", "528 AO-MaxVolts": "5.0000", "XLIGHT Excitation Wheel-State": "2", "470 Shutter-Name": "AnalogIO", "Core-Shutter": "405", "555 AO-Demo": "No", "XLIGHT Dichroic-State": "2", "XYStage-StepSize-Y": "0.1000", "ZPositionUm": 137.405, "XYStage-StepSize-X": "0.1000", "PRIME-FirmwareVersion": "20.28", "PRIME-ClearCycles": "2", "520-Name": "DA Shutter", "405 Shutter-Name": "AnalogIO", "PIEZO-StepSize": "0.0050", "PRIME-ClearMode": "Pre-Exposure", "470 AO-MinVolts": "0.0000", "405 AO-SupportsTriggering": "1", "CameraChannelIndex": 0, "XYStage-StartSpeed": "500.0000", "COM9-StopBits": "2", "405 AO-Description": "NI DAC", "COM4-DelayBetweenCharsMs": "0.0000", "445 AO-Block voltage": "false", "520 AO-TriggerInputLine": "/Dev1/PFI0", "555 AO-Description": "NI DAC", "520 AO-TriggerSampleRate": "100000", "COM4-Verbose": "1", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM4-Name": "COM4", "405 Shutter-Volts": "5.0000", "Vincent-D3-Port": "COM1", "PRIME-PP 4 MIN ADU AFFECTED": "200", "470 Shutter-MinVolts": "0.0000", "PRIME-PP 3 ENABLED": "Yes", "470 AO-Description": "NI DAC", "PRIME-Description": "PVCAM API device adapter", "520 Shutter-MaxVolts": "5.0000", "520 Shutter-Volts": "0.0000", "528 AO-Sequenceable": "1", "PRIME-Binning": "1x1", "555 Shutter-MaxVolts": "5.0000", "528 Shutter-TriggerSequenceLength": "1000", "405 Shutter-Block voltage": "false", "XLIGHT Spin motor-State": "1", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "Vincent-D3-Time to open (ms)": "35.0000", "Vincent-D3-Time to close (ms)": "35.0000", "445 AO-TriggerSampleRate": "100000", "640 Shutter-IOChannel": "Dev1/ao14", "555 AO-Sequenceable": "1", "520 AO-Name": "AnalogIO", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "470 AO-Block voltage": "false", "COM1-Verbose": "1", "COM9-AnswerTimeout": "10000.0000", "528 Shutter-Name": "AnalogIO", "PRIME-Trigger Timeout (secs)": "10", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "NextFrame": 0, "470 AO-TriggerSequenceLength": "1000", "PRIME-TransposeXY": "0", "445 AO-TriggerSequenceLength": "1000", "COM1-Name": "COM1", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "COM9-DataBits": "8", "COM1-AnswerTimeout": "500.0000", "Camera": "", "TE2000-Version": "V2.50-V2.50-V2.10", "528 Shutter-Volts": "0.0000", "XLIGHT Hub-Name": "XLIGHT Hub", "PIEZO-Description": "Ludl stage driver adapter", "PositionName": "Pos3", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSampleRate": "100000", "PRIME-FanSpeedSetpoint": "High", "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "405 AO-TriggerSampleRate": "100000", "470 Shutter-Block voltage": "false", "470-State": "0", "COM3-Parity": "None", "405 AO-Volts": "4.5000", "PRIME-TransposeMirrorY": "0", "PRIME-ChipName": "GS144BSI", "470 AO-Volts": "0.0000", "PRIME-TransposeMirrorX": "0", "445 Shutter-SupportsTriggering": "0", "470 Shutter-SupportsTriggering": "0", "XLIGHT Hub-Description": "XLIGHT hub", "PRIME-PreampOffLimit": "10000", "470 AO-Sequenceable": "1", "528 Shutter-MaxVolts": "5.0000", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "Core-XYStage": "XYStage", "470 AO-TriggerSampleRate": "100000", "PRIME-Timing-PreTriggerDelayNs": "24672000.0000", "528-DA Device": "528 Shutter", "Focus-Description": "Nikon TE2000 focus adapter", "FilterBlock-State": "5", "520 AO-Demo": "No", "COM3-DelayBetweenCharsMs": "0.0000", "470 Shutter-Volts": "0.0000", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "PRIME-PP 2 THRESHOLD": "125", "PRIME-PP 4 THRESHOLD": "75", "XYStage-Acceleration": "4.0000", "470 Shutter-Demo": "No", "PRIME-Name": "PMPCIECam00", "640-DA Device": "640 Shutter", "405 AO-Block voltage": "false", "ElapsedTime-ms": 164467, "PRIME-Offset": "100", "445 AO-IOChannel": "Dev1/ao1", "640 AO-MaxVolts": "5.0000", "PositionIndex": 3, "PRIME-PP 1 ENABLED": "Yes", "COM1-BaudRate": "9600", "640-State": "0", "COM3-Verbose": "1", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "LudlController-TransmissionDelay": "0", "PRIME-FullWellCapacity": "62000", "PRIME-SMARTStreamingEnabled": "No", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "640 Shutter-Description": "NI DAC", "528-Description": "DA device that is used as a shutter", "COM4-Description": "Serial port driver (boost:asio)", "405 Shutter-Description": "NI DAC", "TE2000-Name": "TE2000", "528 Shutter-Description": "NI DAC", "COM9-Description": "Serial port driver (boost:asio)", "LudlController-Name": "LudlController", "445 Shutter-TriggerSequenceLength": "1000", "PRIME-SerialNumber": "A18B203008", "445 AO-Volts": "0.0000", "PRIME-X-dimension": "1200", "PRIME-ShutterCloseDelay": "0", "XLIGHT Disk slider-State": "2", "Position": "Default", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 AO-IOChannel": "Dev1/ao5", "520 AO-IOChannel": "Dev1/ao4", "528 AO-TriggerSequenceLength": "1000", "405 Shutter-MaxVolts": "5.0000", "470-DA Device": "470 Shutter", "PRIME-FTCapable": "No", "Core-ChannelGroup": "Channels", "555 AO-SupportsTriggering": "0", "PRIME-Y-dimension": "1200", "Frame": 0, "555 Shutter-Sequenceable": "1", "PRIME-Timing-ExposureTimeNs": "9992000.0000", "Core-ImageProcessor": "", "528-State": "0", "520 AO-Block voltage": "false", "XLIGHT Excitation Wheel-Label": "QUAD", "640 Shutter-Demo": "No", "COM3-StopBits": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "520 Shutter-IOChannel": "Dev1/ao11", "Width": 1200, "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-SupportsTriggering": "0", "640 AO-TriggerSampleRate": "100000", "520-Description": "DA device that is used as a shutter", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "405 Shutter-Sequenceable": "1", "405 Shutter-SupportsTriggering": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-TriggerSampleRate": "100000", "640 Shutter-MaxVolts": "5.0000", "555 AO-MaxVolts": "5.0000", "520 AO-TriggerSequenceLength": "1000", "COM3-BaudRate": "9600", "COM9-BaudRate": "9600", "Vincent-D3-Channel #": "Ch. #1", "PRIME-PMode": "Normal", "OpticalPath-Label": "Left(100)", "BitDepth": 16, "555 AO-TriggerSequenceLength": "1000", "445 Shutter-Description": "NI DAC", "405 AO-Sequenceable": "1", "PRIME-AcquisitionMethod": "Callbacks", "555 AO-Name": "AnalogIO", "PRIME-ShutterMode": "Pre-Exposure", "COM3-Handshaking": "Off", "445 AO-SupportsTriggering": "0", "ROI": "0-0-1200-1200", "COM9-Verbose": "1", "PRIME-Color": "OFF", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "PRIME-ShutterOpenDelay": "0", "COM1-Parity": "None", "Slice": 1, "SlicePosition": -40, "COM9-DelayBetweenCharsMs": "0.0000", "555-Name": "DA Shutter", "PIEZO-LudlSingleAxisName": "B", "XLIGHT Emission Wheel-Label": "QUAD", "640 Shutter-Name": "AnalogIO", "640 AO-Sequenceable": "1", "520 Shutter-Demo": "No", "528 Shutter-Demo": "No", "470-Name": "DA Shutter", "520-DA Device": "520 Shutter", "XYStage-Description": "Ludl XY stage driver adapter", "445 Shutter-MinVolts": "0.0000", "528 AO-TriggerSampleRate": "100000", "405 AO-MinVolts": "0.0000", "405 Shutter-TriggerSequenceLength": "1000", "Height": 1200, "555 AO-Block voltage": "false", "640 Shutter-Volts": "0.0000", "PixelType": "GRAY16", "445 Shutter-Block voltage": "false", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "520 Shutter-TriggerSequenceLength": "1000", "COM9-Name": "COM9", "Core-SLM": "", "555 AO-Volts": "0.0000", "555 Shutter-Block voltage": "false", "XYStage-Speed": "1800.0000", "520 AO-MaxVolts": "5.0000", "528 Shutter-SupportsTriggering": "0", "COM1-Description": "Serial port driver (boost:asio)", "PRIME-MetadataEnabled": "No", "520 Shutter-MinVolts": "0.0000", "470 Shutter-Sequenceable": "1", "PRIME-PreampDelay": "0", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-MinVolts": "0.0000", "ChannelIndex": 5, "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Core-AutoShutter": "1", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "Exposure-ms": 300, "PRIME-PP 2 ENABLED": "Yes", "640 Shutter-TriggerSampleRate": "100000", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "470 Shutter-IOChannel": "Dev1/ao10", "405 AO-Demo": "No", "PixelSizeUm": 1.1, "470 AO-Demo": "No", "470 Shutter-MaxVolts": "5.0000", "Vincent-D3-Name": "Vincent-D3", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FileName": "img_000000000_P-DAPI-high_001.tif", "LudlController-Reset": "Operate", "PRIME-Exposure": "300.00", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "445-Name": "DA Shutter", "555 Shutter-Name": "AnalogIO", "520 Shutter-TriggerSampleRate": "100000", "445 Shutter-IOChannel": "Dev1/ao9", "PRIME-TransposeCorrection": "0", "XYStage-StepSize": "0.1000", "445-Description": "DA device that is used as a shutter", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 AO-IOChannel": "Dev1/ao7", "520 Shutter-Name": "AnalogIO", "COM3-DataBits": "8", "640 AO-Volts": "0.0000", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "555 Shutter-IOChannel": "Dev1/ao13", "WaitInterval": null, "520 Shutter-Sequenceable": "1", "YPositionUm": 20690.300000000003, "445-State": "0", "445-DA Device": "445 Shutter", "PRIME-CircularBufferAutoSize": "ON", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "555 AO-TriggerSampleRate": "100000", "445 Shutter-Volts": "0.0000", "XLIGHT Hub-Port": "COM3", "Core-AutoFocus": "", "COM4-Handshaking": "Off", "528 Shutter-MinVolts": "0.0000", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "445 Shutter-Name": "AnalogIO", "528 Shutter-Sequenceable": "1", "XYStage-TransposeMirrorX": "0", "XYStage-TransposeMirrorY": "0", "PRIME-ReadoutRate": "100MHz 16bit", "XYStage-Name": "Stage", "640 AO-SupportsTriggering": "0", "640-Name": "DA Shutter", "640 Shutter-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "640 AO-Block voltage": "false", "445 Shutter-Sequenceable": "1", "470 AO-MaxVolts": "5.0000", "COM4-BaudRate": "9600", "470-Description": "DA device that is used as a shutter", "520 Shutter-Description": "NI DAC", "PRIME-CCDTemperature": "-14.9600", "Time": "2019-12-17 21:59:11 -0500", "640 AO-TriggerSequenceLength": "1000", "470 AO-Name": "AnalogIO", "XLIGHT Disk slider-Label": "Disk Pos 40um", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "Binning": "1x1", "TE2000-ModelType": "1", "COM3-AnswerTimeout": "500.0000", "555 Shutter-SupportsTriggering": "0", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "TE2000-Port": "COM4", "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Description": "NI DAC", "COM4-AnswerTimeout": "500.0000", "COM1-DelayBetweenCharsMs": "0.0000", "FilterBlock-Label": "Confocal", "445 AO-Name": "AnalogIO", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-Timing-ReadoutTimeNs": "24672000.0000", "528 AO-Demo": "No", "520 AO-Sequenceable": "1", "555-Description": "DA device that is used as a shutter", "405-DA Device": "405 Shutter", "405 AO-TriggerSequenceLength": "1000", "640 AO-MinVolts": "0.0000", "640 Shutter-Block voltage": "false", "FrameIndex": 0, "PRIME-Port": "CMOS", "555 AO-MinVolts": "0.0000", "COM3-Description": "Serial port driver (boost:asio)", "528 AO-Description": "NI DAC", "555 AO-IOChannel": "Dev1/ao6", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "555 AO-TriggerInputLine": "/Dev1/PFI0", "PRIME-ActualInterval-ms": "374.3700", "LudlController-Port": "COM9", "445 Shutter-Demo": "No", "PRIME-Trigger-Expose Out-Mux": "1", "SliceIndex": 1, "PRIME-CCDTemperatureSetPoint": "-15.0000", "405 Shutter-MinVolts": "0.0000", "PRIME-ExposeOutMode": "First Row", "AxisPositions": null, "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Volts": "0.0000", "528-Name": "DA Shutter", "555-State": "0", "XLIGHT Dichroic-Label": "QUAD", "445 AO-Sequenceable": "1", "405-Name": "DA Shutter", "405 AO-IOChannel": "Dev1/ao0", "Core-Camera": "PRIME", "Focus-Name": "Focus", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "405 Shutter-IOChannel": "Dev1/ao8", "640 AO-Demo": "No", "640 Shutter-SupportsTriggering": "0", "445 AO-Demo": "No", "555-DA Device": "555 Shutter", "470 Shutter-Description": "NI DAC", "528 AO-Volts": "0.0000", "COM1-DataBits": "8", "Vincent-D3-Address": "x", "520 AO-SupportsTriggering": "0", "405-Description": "DA device that is used as a shutter", "640 Shutter-MinVolts": "0.0000", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "405 AO-Name": "AnalogIO", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "640 AO-TriggerInputLine": "/Dev1/PFI0", "405 AO-TriggerInputLine": "/Dev1/PFI0", "640-Description": "DA device that is used as a shutter", "445 Shutter-TriggerSampleRate": "100000", "470 Shutter-TriggerSequenceLength": "1000", "COM1-StopBits": "1", "520 Shutter-SupportsTriggering": "0", "405-State": "1", "Core-Initialize": "1", "XLIGHT Emission Wheel-State": "7", "520 AO-Description": "NI DAC", "528 AO-TriggerInputLine": "/Dev1/PFI0", "445 Shutter-MaxVolts": "5.0000", "COM9-Handshaking": "Off", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "XLIGHT Spin motor-Label": "On (spinning)", "PRIME-PP 5 ENABLED": "No", "UUID": "32300dcc-fc90-4198-b342-cf2b0b1f0c97", "PRIME-TriggerMode": "Internal Trigger", "COM1-Handshaking": "Off", "PRIME-Timing-PostTriggerDelayNs": "49344000.0000", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "Focus-Position": "16479.8000", "528 Shutter-Block voltage": "false", "Channel": "P-DAPI-high", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "555 Shutter-Demo": "No", "405 Shutter-Demo": "No", "528 AO-Name": "AnalogIO", "520-State": "0", "Core-Galvo": "", "405 Shutter-TriggerSampleRate": "100000", "Focus-FocusStepSize_(nm)": "100", "PRIME-PixelType": "16bit", "COM4-DataBits": "8", "470 AO-IOChannel": "Dev1/ao2", "520 AO-MinVolts": "0.0000", "COM9-Parity": "None", "PRIME-CircularBufferEnabled": "ON", "PRIME-PP 6 Reset": "No", "640 AO-Description": "NI DAC", "Source": "PRIME", "445 AO-MinVolts": "0.0000", "PIEZO-Name": "Stage", "445 AO-Description": "NI DAC", "PRIME-CircularBufferFrameCount": "46", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "520 AO-Volts": "0.0000", "528 Shutter-TriggerSampleRate": "100000", "XPositionUm": 62321, "OpticalPath-State": "4", "PRIME-PP 3 THRESHOLD": "80", "PRIME-PVCAM Version": "3.7.1", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Gain": "1-HDR", "640 Shutter-TriggerSequenceLength": "1000", "528 AO-SupportsTriggering": "0", "445 AO-TriggerInputLine": "/Dev1/PFI0", "OpticalPath-Name": "OpticalPath", "PRIME-PP 4 ENABLED": "Yes", "PRIME-PVCAM Adapter Version": "1.3.29", "COM3-Name": "COM3", "405 AO-MaxVolts": "5.0000", "520 Shutter-Block voltage": "false", "PRIME-Timing-ClearingTimeNs": "49344000.0000", "Core-Focus": "PIEZO", "528 AO-Block voltage": "false", "TE2000-Description": "Nikon TE2000 hub", "528 AO-MinVolts": "0.0000", "COM4-Parity": "None", "Summary": { "Slices": 11, "ChContrastMin": [ 0, 0, 0, 0, 0, 0 ], "PixelAspect": 1, "SlicesFirst": true, "Interval_ms": 0, "Time": "2019-12-17 21:58:39 -0500", "Prefix": "seq_3", "InitialPositionList": [ { "Label": "Pos0", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.435], "Focus": [16480.2], "XYStage": [ 69822.40000000001, 20100.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos1", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.425], "Focus": [16480.3], "XYStage": [ 69004, 20101 ] }, "GridColumnIndex": 0 }, { "Label": "Pos2", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.44], "Focus": [16480.1], "XYStage": [ 63119.5, 20690.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos3", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.405], "Focus": [16480.2], "XYStage": [ 62321, 20690.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos4", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.415], "Focus": [16480], "XYStage": [ 56604.100000000006, 20688.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos5", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.42000000000002], "Focus": [16480.1], "XYStage": [ 56034.200000000004, 20688.800000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos6", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.455], "Focus": [16480], "XYStage": [ 50889.3, 20496.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos7", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.43], "Focus": [16480.2], "XYStage": [ 50101.600000000006, 20496.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos8", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.67000000000002], "Focus": [16479.9], "XYStage": [ 44818.600000000006, 20496.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos9", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.67000000000002], "Focus": [16480], "XYStage": [ 43897.9, 20436.800000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos10", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.675], "Focus": [16479.9], "XYStage": [ 39670.600000000006, 20664.9 ] }, "GridColumnIndex": 0 }, { "Label": "Pos11", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.69], "Focus": [16479.9], "XYStage": [ 38734, 20664.600000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos12", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.695], "Focus": [16480.1], "XYStage": [ 34037.200000000004, 20664.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos13", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.685], "Focus": [16480.1], "XYStage": [ 33152, 20664.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos14", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.69], "Focus": [16480.1], "XYStage": [ 28122.800000000003, 20664.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos15", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.675], "Focus": [16480], "XYStage": [ 27422.9, 20664.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos16", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.64000000000001], "Focus": [16480], "XYStage": [ 23005.2, 20664.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos17", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.69], "Focus": [16479.9], "XYStage": [ 21820.600000000002, 20409.600000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos18", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.41], "Focus": [16480.1], "XYStage": [ 73697.6, -13222.800000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos19", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.455], "Focus": [16480.1], "XYStage": [ 72765.8, -13223 ] }, "GridColumnIndex": 0 }, { "Label": "Pos20", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.4], "Focus": [16480.2], "XYStage": [ 67445.5, -12303.800000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos21", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.415], "Focus": [16480.2], "XYStage": [ 66613.3, -12303.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos22", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.415], "Focus": [16480.1], "XYStage": [ 61819.4, -11936.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos23", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.405], "Focus": [16480.2], "XYStage": [ 61325.700000000004, -11937.1 ] }, "GridColumnIndex": 0 }, { "Label": "Pos24", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.46], "Focus": [16480.2], "XYStage": [ 55377, -10974.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos25", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.445], "Focus": [16480.1], "XYStage": [ 54827, -11214.800000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos26", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.435], "Focus": [16480.2], "XYStage": [ 49937.100000000006, -10919.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos27", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.425], "Focus": [16480.1], "XYStage": [ 48764, -10919.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos28", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.42000000000002], "Focus": [16480.2], "XYStage": [ 43978.4, -10920.400000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos29", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.435], "Focus": [16480.2], "XYStage": [ 43106.3, -10920.400000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos30", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.405], "Focus": [16480.3], "XYStage": [ 38212.4, -10920.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos31", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.445], "Focus": [16480.2], "XYStage": [ 37254.9, -10920.400000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos32", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.44], "Focus": [16480.3], "XYStage": [ 32472.800000000003, -11294.400000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos33", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [166.95000000000002], "Focus": [16480.2], "XYStage": [ 31420.7, -11294.400000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos34", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [166.95000000000002], "Focus": [16480.2], "XYStage": [ 26881.5, -11640 ] }, "GridColumnIndex": 0 }, { "Label": "Pos35", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [166.98], "Focus": [16480.2], "XYStage": [ 25876.100000000002, -11641.1 ] }, "GridColumnIndex": 0 } ], "z-step_um": 10, "GridRow": 0, "BitDepth": 16, "Depth": 2, "Channels": 6, "Source": "Micro-Manager", "ChColors": [ -16711681, -256, -65281, -1, -1, -16776961 ], "MetadataVersion": 10, "KeepShutterOpenChannels": true, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536, 65536 ], "CustomIntervals_ms": [], "Height": 1200, "MicroManagerVersion": "1.4.23 20180219", "GridColumn": 0, "UUID": "8c8f9d20-b128-4577-9f91-89e70f22d319", "PixelType": "GRAY16", "Width": 1200, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\Yan\\20191217-YC113-S5S6", "Frames": 1, "Comment": "", "UserName": "Nikon", "PixelSize_um": 1.1, "ROI": [ 0, 0, 1200, 1200 ], "Date": "2019-12-17", "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC", "P-DAPI-high" ], "KeepShutterOpenSlices": true, "IJType": 1, "ComputerName": "ZadorConfocal2", "Positions": 36, "PositionIndex": 3, "CameraTimeout": 20000, "TimeFirst": false }, "640 AO-Name": "AnalogIO", "Vincent-D3-Command": "Close" }, "FrameKey-0-5-2": { "COM4-StopBits": "1", "528 AO-MaxVolts": "5.0000", "XLIGHT Excitation Wheel-State": "2", "470 Shutter-Name": "AnalogIO", "Core-Shutter": "405", "555 AO-Demo": "No", "XLIGHT Dichroic-State": "2", "XYStage-StepSize-Y": "0.1000", "ZPositionUm": 147.405, "XYStage-StepSize-X": "0.1000", "PRIME-FirmwareVersion": "20.28", "PRIME-ClearCycles": "2", "520-Name": "DA Shutter", "405 Shutter-Name": "AnalogIO", "PIEZO-StepSize": "0.0050", "PRIME-ClearMode": "Pre-Exposure", "470 AO-MinVolts": "0.0000", "405 AO-SupportsTriggering": "1", "CameraChannelIndex": 0, "XYStage-StartSpeed": "500.0000", "COM9-StopBits": "2", "405 AO-Description": "NI DAC", "COM4-DelayBetweenCharsMs": "0.0000", "445 AO-Block voltage": "false", "520 AO-TriggerInputLine": "/Dev1/PFI0", "555 AO-Description": "NI DAC", "520 AO-TriggerSampleRate": "100000", "COM4-Verbose": "1", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM4-Name": "COM4", "405 Shutter-Volts": "5.0000", "Vincent-D3-Port": "COM1", "PRIME-PP 4 MIN ADU AFFECTED": "200", "470 Shutter-MinVolts": "0.0000", "PRIME-PP 3 ENABLED": "Yes", "470 AO-Description": "NI DAC", "PRIME-Description": "PVCAM API device adapter", "520 Shutter-MaxVolts": "5.0000", "520 Shutter-Volts": "0.0000", "528 AO-Sequenceable": "1", "PRIME-Binning": "1x1", "555 Shutter-MaxVolts": "5.0000", "528 Shutter-TriggerSequenceLength": "1000", "405 Shutter-Block voltage": "false", "XLIGHT Spin motor-State": "1", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "Vincent-D3-Time to open (ms)": "35.0000", "Vincent-D3-Time to close (ms)": "35.0000", "445 AO-TriggerSampleRate": "100000", "640 Shutter-IOChannel": "Dev1/ao14", "555 AO-Sequenceable": "1", "520 AO-Name": "AnalogIO", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "470 AO-Block voltage": "false", "COM1-Verbose": "1", "COM9-AnswerTimeout": "10000.0000", "528 Shutter-Name": "AnalogIO", "PRIME-Trigger Timeout (secs)": "10", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "NextFrame": 0, "470 AO-TriggerSequenceLength": "1000", "PRIME-TransposeXY": "0", "445 AO-TriggerSequenceLength": "1000", "COM1-Name": "COM1", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "COM9-DataBits": "8", "COM1-AnswerTimeout": "500.0000", "Camera": "", "TE2000-Version": "V2.50-V2.50-V2.10", "528 Shutter-Volts": "0.0000", "XLIGHT Hub-Name": "XLIGHT Hub", "PIEZO-Description": "Ludl stage driver adapter", "PositionName": "Pos3", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSampleRate": "100000", "PRIME-FanSpeedSetpoint": "High", "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "405 AO-TriggerSampleRate": "100000", "470 Shutter-Block voltage": "false", "470-State": "0", "COM3-Parity": "None", "405 AO-Volts": "4.5000", "PRIME-TransposeMirrorY": "0", "PRIME-ChipName": "GS144BSI", "470 AO-Volts": "0.0000", "PRIME-TransposeMirrorX": "0", "445 Shutter-SupportsTriggering": "0", "470 Shutter-SupportsTriggering": "0", "XLIGHT Hub-Description": "XLIGHT hub", "PRIME-PreampOffLimit": "10000", "470 AO-Sequenceable": "1", "528 Shutter-MaxVolts": "5.0000", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "Core-XYStage": "XYStage", "470 AO-TriggerSampleRate": "100000", "PRIME-Timing-PreTriggerDelayNs": "24672000.0000", "528-DA Device": "528 Shutter", "Focus-Description": "Nikon TE2000 focus adapter", "FilterBlock-State": "5", "520 AO-Demo": "No", "COM3-DelayBetweenCharsMs": "0.0000", "470 Shutter-Volts": "0.0000", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "PRIME-PP 2 THRESHOLD": "125", "PRIME-PP 4 THRESHOLD": "75", "XYStage-Acceleration": "4.0000", "470 Shutter-Demo": "No", "PRIME-Name": "PMPCIECam00", "640-DA Device": "640 Shutter", "405 AO-Block voltage": "false", "ElapsedTime-ms": 165003, "PRIME-Offset": "100", "445 AO-IOChannel": "Dev1/ao1", "640 AO-MaxVolts": "5.0000", "PositionIndex": 3, "PRIME-PP 1 ENABLED": "Yes", "COM1-BaudRate": "9600", "640-State": "0", "COM3-Verbose": "1", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "LudlController-TransmissionDelay": "0", "PRIME-FullWellCapacity": "62000", "PRIME-SMARTStreamingEnabled": "No", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "640 Shutter-Description": "NI DAC", "528-Description": "DA device that is used as a shutter", "COM4-Description": "Serial port driver (boost:asio)", "405 Shutter-Description": "NI DAC", "TE2000-Name": "TE2000", "528 Shutter-Description": "NI DAC", "COM9-Description": "Serial port driver (boost:asio)", "LudlController-Name": "LudlController", "445 Shutter-TriggerSequenceLength": "1000", "PRIME-SerialNumber": "A18B203008", "445 AO-Volts": "0.0000", "PRIME-X-dimension": "1200", "PRIME-ShutterCloseDelay": "0", "XLIGHT Disk slider-State": "2", "Position": "Default", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 AO-IOChannel": "Dev1/ao5", "520 AO-IOChannel": "Dev1/ao4", "528 AO-TriggerSequenceLength": "1000", "405 Shutter-MaxVolts": "5.0000", "470-DA Device": "470 Shutter", "PRIME-FTCapable": "No", "Core-ChannelGroup": "Channels", "555 AO-SupportsTriggering": "0", "PRIME-Y-dimension": "1200", "Frame": 0, "555 Shutter-Sequenceable": "1", "PRIME-Timing-ExposureTimeNs": "9992000.0000", "Core-ImageProcessor": "", "528-State": "0", "520 AO-Block voltage": "false", "XLIGHT Excitation Wheel-Label": "QUAD", "640 Shutter-Demo": "No", "COM3-StopBits": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "520 Shutter-IOChannel": "Dev1/ao11", "Width": 1200, "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-SupportsTriggering": "0", "640 AO-TriggerSampleRate": "100000", "520-Description": "DA device that is used as a shutter", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "405 Shutter-Sequenceable": "1", "405 Shutter-SupportsTriggering": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-TriggerSampleRate": "100000", "640 Shutter-MaxVolts": "5.0000", "555 AO-MaxVolts": "5.0000", "520 AO-TriggerSequenceLength": "1000", "COM3-BaudRate": "9600", "COM9-BaudRate": "9600", "Vincent-D3-Channel #": "Ch. #1", "PRIME-PMode": "Normal", "OpticalPath-Label": "Left(100)", "BitDepth": 16, "555 AO-TriggerSequenceLength": "1000", "445 Shutter-Description": "NI DAC", "405 AO-Sequenceable": "1", "PRIME-AcquisitionMethod": "Callbacks", "555 AO-Name": "AnalogIO", "PRIME-ShutterMode": "Pre-Exposure", "COM3-Handshaking": "Off", "445 AO-SupportsTriggering": "0", "ROI": "0-0-1200-1200", "COM9-Verbose": "1", "PRIME-Color": "OFF", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "PRIME-ShutterOpenDelay": "0", "COM1-Parity": "None", "Slice": 2, "SlicePosition": -30, "COM9-DelayBetweenCharsMs": "0.0000", "555-Name": "DA Shutter", "PIEZO-LudlSingleAxisName": "B", "XLIGHT Emission Wheel-Label": "QUAD", "640 Shutter-Name": "AnalogIO", "640 AO-Sequenceable": "1", "520 Shutter-Demo": "No", "528 Shutter-Demo": "No", "470-Name": "DA Shutter", "520-DA Device": "520 Shutter", "XYStage-Description": "Ludl XY stage driver adapter", "445 Shutter-MinVolts": "0.0000", "528 AO-TriggerSampleRate": "100000", "405 AO-MinVolts": "0.0000", "405 Shutter-TriggerSequenceLength": "1000", "Height": 1200, "555 AO-Block voltage": "false", "640 Shutter-Volts": "0.0000", "PixelType": "GRAY16", "445 Shutter-Block voltage": "false", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "520 Shutter-TriggerSequenceLength": "1000", "COM9-Name": "COM9", "Core-SLM": "", "555 AO-Volts": "0.0000", "555 Shutter-Block voltage": "false", "XYStage-Speed": "1800.0000", "520 AO-MaxVolts": "5.0000", "528 Shutter-SupportsTriggering": "0", "COM1-Description": "Serial port driver (boost:asio)", "PRIME-MetadataEnabled": "No", "520 Shutter-MinVolts": "0.0000", "470 Shutter-Sequenceable": "1", "PRIME-PreampDelay": "0", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-MinVolts": "0.0000", "ChannelIndex": 5, "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Core-AutoShutter": "1", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "Exposure-ms": 300, "PRIME-PP 2 ENABLED": "Yes", "640 Shutter-TriggerSampleRate": "100000", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "470 Shutter-IOChannel": "Dev1/ao10", "405 AO-Demo": "No", "PixelSizeUm": 1.1, "470 AO-Demo": "No", "470 Shutter-MaxVolts": "5.0000", "Vincent-D3-Name": "Vincent-D3", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FileName": "img_000000000_P-DAPI-high_002.tif", "LudlController-Reset": "Operate", "PRIME-Exposure": "300.00", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "445-Name": "DA Shutter", "555 Shutter-Name": "AnalogIO", "520 Shutter-TriggerSampleRate": "100000", "445 Shutter-IOChannel": "Dev1/ao9", "PRIME-TransposeCorrection": "0", "XYStage-StepSize": "0.1000", "445-Description": "DA device that is used as a shutter", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 AO-IOChannel": "Dev1/ao7", "520 Shutter-Name": "AnalogIO", "COM3-DataBits": "8", "640 AO-Volts": "0.0000", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "555 Shutter-IOChannel": "Dev1/ao13", "WaitInterval": null, "520 Shutter-Sequenceable": "1", "YPositionUm": 20690.300000000003, "445-State": "0", "445-DA Device": "445 Shutter", "PRIME-CircularBufferAutoSize": "ON", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "555 AO-TriggerSampleRate": "100000", "445 Shutter-Volts": "0.0000", "XLIGHT Hub-Port": "COM3", "Core-AutoFocus": "", "COM4-Handshaking": "Off", "528 Shutter-MinVolts": "0.0000", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "445 Shutter-Name": "AnalogIO", "528 Shutter-Sequenceable": "1", "XYStage-TransposeMirrorX": "0", "XYStage-TransposeMirrorY": "0", "PRIME-ReadoutRate": "100MHz 16bit", "XYStage-Name": "Stage", "640 AO-SupportsTriggering": "0", "640-Name": "DA Shutter", "640 Shutter-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "640 AO-Block voltage": "false", "445 Shutter-Sequenceable": "1", "470 AO-MaxVolts": "5.0000", "COM4-BaudRate": "9600", "470-Description": "DA device that is used as a shutter", "520 Shutter-Description": "NI DAC", "PRIME-CCDTemperature": "-14.9600", "Time": "2019-12-17 21:59:12 -0500", "640 AO-TriggerSequenceLength": "1000", "470 AO-Name": "AnalogIO", "XLIGHT Disk slider-Label": "Disk Pos 40um", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "Binning": "1x1", "TE2000-ModelType": "1", "COM3-AnswerTimeout": "500.0000", "555 Shutter-SupportsTriggering": "0", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "TE2000-Port": "COM4", "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Description": "NI DAC", "COM4-AnswerTimeout": "500.0000", "COM1-DelayBetweenCharsMs": "0.0000", "FilterBlock-Label": "Confocal", "445 AO-Name": "AnalogIO", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-Timing-ReadoutTimeNs": "24672000.0000", "528 AO-Demo": "No", "520 AO-Sequenceable": "1", "555-Description": "DA device that is used as a shutter", "405-DA Device": "405 Shutter", "405 AO-TriggerSequenceLength": "1000", "640 AO-MinVolts": "0.0000", "640 Shutter-Block voltage": "false", "FrameIndex": 0, "PRIME-Port": "CMOS", "555 AO-MinVolts": "0.0000", "COM3-Description": "Serial port driver (boost:asio)", "528 AO-Description": "NI DAC", "555 AO-IOChannel": "Dev1/ao6", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "555 AO-TriggerInputLine": "/Dev1/PFI0", "PRIME-ActualInterval-ms": "374.3700", "LudlController-Port": "COM9", "445 Shutter-Demo": "No", "PRIME-Trigger-Expose Out-Mux": "1", "SliceIndex": 2, "PRIME-CCDTemperatureSetPoint": "-15.0000", "405 Shutter-MinVolts": "0.0000", "PRIME-ExposeOutMode": "First Row", "AxisPositions": null, "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Volts": "0.0000", "528-Name": "DA Shutter", "555-State": "0", "XLIGHT Dichroic-Label": "QUAD", "445 AO-Sequenceable": "1", "405-Name": "DA Shutter", "405 AO-IOChannel": "Dev1/ao0", "Core-Camera": "PRIME", "Focus-Name": "Focus", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "405 Shutter-IOChannel": "Dev1/ao8", "640 AO-Demo": "No", "640 Shutter-SupportsTriggering": "0", "445 AO-Demo": "No", "555-DA Device": "555 Shutter", "470 Shutter-Description": "NI DAC", "528 AO-Volts": "0.0000", "COM1-DataBits": "8", "Vincent-D3-Address": "x", "520 AO-SupportsTriggering": "0", "405-Description": "DA device that is used as a shutter", "640 Shutter-MinVolts": "0.0000", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "405 AO-Name": "AnalogIO", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "640 AO-TriggerInputLine": "/Dev1/PFI0", "405 AO-TriggerInputLine": "/Dev1/PFI0", "640-Description": "DA device that is used as a shutter", "445 Shutter-TriggerSampleRate": "100000", "470 Shutter-TriggerSequenceLength": "1000", "COM1-StopBits": "1", "520 Shutter-SupportsTriggering": "0", "405-State": "1", "Core-Initialize": "1", "XLIGHT Emission Wheel-State": "7", "520 AO-Description": "NI DAC", "528 AO-TriggerInputLine": "/Dev1/PFI0", "445 Shutter-MaxVolts": "5.0000", "COM9-Handshaking": "Off", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "XLIGHT Spin motor-Label": "On (spinning)", "PRIME-PP 5 ENABLED": "No", "UUID": "34c910bf-cab0-4488-b0ec-5e3d9f27b9c0", "PRIME-TriggerMode": "Internal Trigger", "COM1-Handshaking": "Off", "PRIME-Timing-PostTriggerDelayNs": "49344000.0000", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "Focus-Position": "16479.8000", "528 Shutter-Block voltage": "false", "Channel": "P-DAPI-high", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "555 Shutter-Demo": "No", "405 Shutter-Demo": "No", "528 AO-Name": "AnalogIO", "520-State": "0", "Core-Galvo": "", "405 Shutter-TriggerSampleRate": "100000", "Focus-FocusStepSize_(nm)": "100", "PRIME-PixelType": "16bit", "COM4-DataBits": "8", "470 AO-IOChannel": "Dev1/ao2", "520 AO-MinVolts": "0.0000", "COM9-Parity": "None", "PRIME-CircularBufferEnabled": "ON", "PRIME-PP 6 Reset": "No", "640 AO-Description": "NI DAC", "Source": "PRIME", "445 AO-MinVolts": "0.0000", "PIEZO-Name": "Stage", "445 AO-Description": "NI DAC", "PRIME-CircularBufferFrameCount": "46", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "520 AO-Volts": "0.0000", "528 Shutter-TriggerSampleRate": "100000", "XPositionUm": 62321, "OpticalPath-State": "4", "PRIME-PP 3 THRESHOLD": "80", "PRIME-PVCAM Version": "3.7.1", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Gain": "1-HDR", "640 Shutter-TriggerSequenceLength": "1000", "528 AO-SupportsTriggering": "0", "445 AO-TriggerInputLine": "/Dev1/PFI0", "OpticalPath-Name": "OpticalPath", "PRIME-PP 4 ENABLED": "Yes", "PRIME-PVCAM Adapter Version": "1.3.29", "COM3-Name": "COM3", "405 AO-MaxVolts": "5.0000", "520 Shutter-Block voltage": "false", "PRIME-Timing-ClearingTimeNs": "49344000.0000", "Core-Focus": "PIEZO", "528 AO-Block voltage": "false", "TE2000-Description": "Nikon TE2000 hub", "528 AO-MinVolts": "0.0000", "COM4-Parity": "None", "Summary": { "Slices": 11, "ChContrastMin": [ 0, 0, 0, 0, 0, 0 ], "PixelAspect": 1, "SlicesFirst": true, "Interval_ms": 0, "Time": "2019-12-17 21:58:39 -0500", "Prefix": "seq_3", "InitialPositionList": [ { "Label": "Pos0", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.435], "Focus": [16480.2], "XYStage": [ 69822.40000000001, 20100.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos1", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.425], "Focus": [16480.3], "XYStage": [ 69004, 20101 ] }, "GridColumnIndex": 0 }, { "Label": "Pos2", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.44], "Focus": [16480.1], "XYStage": [ 63119.5, 20690.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos3", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.405], "Focus": [16480.2], "XYStage": [ 62321, 20690.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos4", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.415], "Focus": [16480], "XYStage": [ 56604.100000000006, 20688.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos5", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.42000000000002], "Focus": [16480.1], "XYStage": [ 56034.200000000004, 20688.800000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos6", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.455], "Focus": [16480], "XYStage": [ 50889.3, 20496.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos7", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.43], "Focus": [16480.2], "XYStage": [ 50101.600000000006, 20496.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos8", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.67000000000002], "Focus": [16479.9], "XYStage": [ 44818.600000000006, 20496.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos9", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.67000000000002], "Focus": [16480], "XYStage": [ 43897.9, 20436.800000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos10", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.675], "Focus": [16479.9], "XYStage": [ 39670.600000000006, 20664.9 ] }, "GridColumnIndex": 0 }, { "Label": "Pos11", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.69], "Focus": [16479.9], "XYStage": [ 38734, 20664.600000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos12", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.695], "Focus": [16480.1], "XYStage": [ 34037.200000000004, 20664.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos13", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.685], "Focus": [16480.1], "XYStage": [ 33152, 20664.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos14", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.69], "Focus": [16480.1], "XYStage": [ 28122.800000000003, 20664.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos15", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.675], "Focus": [16480], "XYStage": [ 27422.9, 20664.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos16", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.64000000000001], "Focus": [16480], "XYStage": [ 23005.2, 20664.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos17", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.69], "Focus": [16479.9], "XYStage": [ 21820.600000000002, 20409.600000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos18", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.41], "Focus": [16480.1], "XYStage": [ 73697.6, -13222.800000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos19", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.455], "Focus": [16480.1], "XYStage": [ 72765.8, -13223 ] }, "GridColumnIndex": 0 }, { "Label": "Pos20", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.4], "Focus": [16480.2], "XYStage": [ 67445.5, -12303.800000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos21", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.415], "Focus": [16480.2], "XYStage": [ 66613.3, -12303.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos22", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.415], "Focus": [16480.1], "XYStage": [ 61819.4, -11936.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos23", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.405], "Focus": [16480.2], "XYStage": [ 61325.700000000004, -11937.1 ] }, "GridColumnIndex": 0 }, { "Label": "Pos24", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.46], "Focus": [16480.2], "XYStage": [ 55377, -10974.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos25", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.445], "Focus": [16480.1], "XYStage": [ 54827, -11214.800000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos26", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.435], "Focus": [16480.2], "XYStage": [ 49937.100000000006, -10919.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos27", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.425], "Focus": [16480.1], "XYStage": [ 48764, -10919.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos28", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.42000000000002], "Focus": [16480.2], "XYStage": [ 43978.4, -10920.400000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos29", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.435], "Focus": [16480.2], "XYStage": [ 43106.3, -10920.400000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos30", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.405], "Focus": [16480.3], "XYStage": [ 38212.4, -10920.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos31", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.445], "Focus": [16480.2], "XYStage": [ 37254.9, -10920.400000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos32", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.44], "Focus": [16480.3], "XYStage": [ 32472.800000000003, -11294.400000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos33", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [166.95000000000002], "Focus": [16480.2], "XYStage": [ 31420.7, -11294.400000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos34", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [166.95000000000002], "Focus": [16480.2], "XYStage": [ 26881.5, -11640 ] }, "GridColumnIndex": 0 }, { "Label": "Pos35", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [166.98], "Focus": [16480.2], "XYStage": [ 25876.100000000002, -11641.1 ] }, "GridColumnIndex": 0 } ], "z-step_um": 10, "GridRow": 0, "BitDepth": 16, "Depth": 2, "Channels": 6, "Source": "Micro-Manager", "ChColors": [ -16711681, -256, -65281, -1, -1, -16776961 ], "MetadataVersion": 10, "KeepShutterOpenChannels": true, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536, 65536 ], "CustomIntervals_ms": [], "Height": 1200, "MicroManagerVersion": "1.4.23 20180219", "GridColumn": 0, "UUID": "8c8f9d20-b128-4577-9f91-89e70f22d319", "PixelType": "GRAY16", "Width": 1200, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\Yan\\20191217-YC113-S5S6", "Frames": 1, "Comment": "", "UserName": "Nikon", "PixelSize_um": 1.1, "ROI": [ 0, 0, 1200, 1200 ], "Date": "2019-12-17", "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC", "P-DAPI-high" ], "KeepShutterOpenSlices": true, "IJType": 1, "ComputerName": "ZadorConfocal2", "Positions": 36, "PositionIndex": 3, "CameraTimeout": 20000, "TimeFirst": false }, "640 AO-Name": "AnalogIO", "Vincent-D3-Command": "Close" }, "FrameKey-0-5-3": { "COM4-StopBits": "1", "528 AO-MaxVolts": "5.0000", "XLIGHT Excitation Wheel-State": "2", "470 Shutter-Name": "AnalogIO", "Core-Shutter": "405", "555 AO-Demo": "No", "XLIGHT Dichroic-State": "2", "XYStage-StepSize-Y": "0.1000", "ZPositionUm": 157.405, "XYStage-StepSize-X": "0.1000", "PRIME-FirmwareVersion": "20.28", "PRIME-ClearCycles": "2", "520-Name": "DA Shutter", "405 Shutter-Name": "AnalogIO", "PIEZO-StepSize": "0.0050", "PRIME-ClearMode": "Pre-Exposure", "470 AO-MinVolts": "0.0000", "405 AO-SupportsTriggering": "1", "CameraChannelIndex": 0, "XYStage-StartSpeed": "500.0000", "COM9-StopBits": "2", "405 AO-Description": "NI DAC", "COM4-DelayBetweenCharsMs": "0.0000", "445 AO-Block voltage": "false", "520 AO-TriggerInputLine": "/Dev1/PFI0", "555 AO-Description": "NI DAC", "520 AO-TriggerSampleRate": "100000", "COM4-Verbose": "1", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM4-Name": "COM4", "405 Shutter-Volts": "5.0000", "Vincent-D3-Port": "COM1", "PRIME-PP 4 MIN ADU AFFECTED": "200", "470 Shutter-MinVolts": "0.0000", "PRIME-PP 3 ENABLED": "Yes", "470 AO-Description": "NI DAC", "PRIME-Description": "PVCAM API device adapter", "520 Shutter-MaxVolts": "5.0000", "520 Shutter-Volts": "0.0000", "528 AO-Sequenceable": "1", "PRIME-Binning": "1x1", "555 Shutter-MaxVolts": "5.0000", "528 Shutter-TriggerSequenceLength": "1000", "405 Shutter-Block voltage": "false", "XLIGHT Spin motor-State": "1", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "Vincent-D3-Time to open (ms)": "35.0000", "Vincent-D3-Time to close (ms)": "35.0000", "445 AO-TriggerSampleRate": "100000", "640 Shutter-IOChannel": "Dev1/ao14", "555 AO-Sequenceable": "1", "520 AO-Name": "AnalogIO", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "470 AO-Block voltage": "false", "COM1-Verbose": "1", "COM9-AnswerTimeout": "10000.0000", "528 Shutter-Name": "AnalogIO", "PRIME-Trigger Timeout (secs)": "10", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "NextFrame": 0, "470 AO-TriggerSequenceLength": "1000", "PRIME-TransposeXY": "0", "445 AO-TriggerSequenceLength": "1000", "COM1-Name": "COM1", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "COM9-DataBits": "8", "COM1-AnswerTimeout": "500.0000", "Camera": "", "TE2000-Version": "V2.50-V2.50-V2.10", "528 Shutter-Volts": "0.0000", "XLIGHT Hub-Name": "XLIGHT Hub", "PIEZO-Description": "Ludl stage driver adapter", "PositionName": "Pos3", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSampleRate": "100000", "PRIME-FanSpeedSetpoint": "High", "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "405 AO-TriggerSampleRate": "100000", "470 Shutter-Block voltage": "false", "470-State": "0", "COM3-Parity": "None", "405 AO-Volts": "4.5000", "PRIME-TransposeMirrorY": "0", "PRIME-ChipName": "GS144BSI", "470 AO-Volts": "0.0000", "PRIME-TransposeMirrorX": "0", "445 Shutter-SupportsTriggering": "0", "470 Shutter-SupportsTriggering": "0", "XLIGHT Hub-Description": "XLIGHT hub", "PRIME-PreampOffLimit": "10000", "470 AO-Sequenceable": "1", "528 Shutter-MaxVolts": "5.0000", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "Core-XYStage": "XYStage", "470 AO-TriggerSampleRate": "100000", "PRIME-Timing-PreTriggerDelayNs": "24672000.0000", "528-DA Device": "528 Shutter", "Focus-Description": "Nikon TE2000 focus adapter", "FilterBlock-State": "5", "520 AO-Demo": "No", "COM3-DelayBetweenCharsMs": "0.0000", "470 Shutter-Volts": "0.0000", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "PRIME-PP 2 THRESHOLD": "125", "PRIME-PP 4 THRESHOLD": "75", "XYStage-Acceleration": "4.0000", "470 Shutter-Demo": "No", "PRIME-Name": "PMPCIECam00", "640-DA Device": "640 Shutter", "405 AO-Block voltage": "false", "ElapsedTime-ms": 165546, "PRIME-Offset": "100", "445 AO-IOChannel": "Dev1/ao1", "640 AO-MaxVolts": "5.0000", "PositionIndex": 3, "PRIME-PP 1 ENABLED": "Yes", "COM1-BaudRate": "9600", "640-State": "0", "COM3-Verbose": "1", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "LudlController-TransmissionDelay": "0", "PRIME-FullWellCapacity": "62000", "PRIME-SMARTStreamingEnabled": "No", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "640 Shutter-Description": "NI DAC", "528-Description": "DA device that is used as a shutter", "COM4-Description": "Serial port driver (boost:asio)", "405 Shutter-Description": "NI DAC", "TE2000-Name": "TE2000", "528 Shutter-Description": "NI DAC", "COM9-Description": "Serial port driver (boost:asio)", "LudlController-Name": "LudlController", "445 Shutter-TriggerSequenceLength": "1000", "PRIME-SerialNumber": "A18B203008", "445 AO-Volts": "0.0000", "PRIME-X-dimension": "1200", "PRIME-ShutterCloseDelay": "0", "XLIGHT Disk slider-State": "2", "Position": "Default", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 AO-IOChannel": "Dev1/ao5", "520 AO-IOChannel": "Dev1/ao4", "528 AO-TriggerSequenceLength": "1000", "405 Shutter-MaxVolts": "5.0000", "470-DA Device": "470 Shutter", "PRIME-FTCapable": "No", "Core-ChannelGroup": "Channels", "555 AO-SupportsTriggering": "0", "PRIME-Y-dimension": "1200", "Frame": 0, "555 Shutter-Sequenceable": "1", "PRIME-Timing-ExposureTimeNs": "9992000.0000", "Core-ImageProcessor": "", "528-State": "0", "520 AO-Block voltage": "false", "XLIGHT Excitation Wheel-Label": "QUAD", "640 Shutter-Demo": "No", "COM3-StopBits": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "520 Shutter-IOChannel": "Dev1/ao11", "Width": 1200, "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-SupportsTriggering": "0", "640 AO-TriggerSampleRate": "100000", "520-Description": "DA device that is used as a shutter", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "405 Shutter-Sequenceable": "1", "405 Shutter-SupportsTriggering": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-TriggerSampleRate": "100000", "640 Shutter-MaxVolts": "5.0000", "555 AO-MaxVolts": "5.0000", "520 AO-TriggerSequenceLength": "1000", "COM3-BaudRate": "9600", "COM9-BaudRate": "9600", "Vincent-D3-Channel #": "Ch. #1", "PRIME-PMode": "Normal", "OpticalPath-Label": "Left(100)", "BitDepth": 16, "555 AO-TriggerSequenceLength": "1000", "445 Shutter-Description": "NI DAC", "405 AO-Sequenceable": "1", "PRIME-AcquisitionMethod": "Callbacks", "555 AO-Name": "AnalogIO", "PRIME-ShutterMode": "Pre-Exposure", "COM3-Handshaking": "Off", "445 AO-SupportsTriggering": "0", "ROI": "0-0-1200-1200", "COM9-Verbose": "1", "PRIME-Color": "OFF", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "PRIME-ShutterOpenDelay": "0", "COM1-Parity": "None", "Slice": 3, "SlicePosition": -20, "COM9-DelayBetweenCharsMs": "0.0000", "555-Name": "DA Shutter", "PIEZO-LudlSingleAxisName": "B", "XLIGHT Emission Wheel-Label": "QUAD", "640 Shutter-Name": "AnalogIO", "640 AO-Sequenceable": "1", "520 Shutter-Demo": "No", "528 Shutter-Demo": "No", "470-Name": "DA Shutter", "520-DA Device": "520 Shutter", "XYStage-Description": "Ludl XY stage driver adapter", "445 Shutter-MinVolts": "0.0000", "528 AO-TriggerSampleRate": "100000", "405 AO-MinVolts": "0.0000", "405 Shutter-TriggerSequenceLength": "1000", "Height": 1200, "555 AO-Block voltage": "false", "640 Shutter-Volts": "0.0000", "PixelType": "GRAY16", "445 Shutter-Block voltage": "false", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "520 Shutter-TriggerSequenceLength": "1000", "COM9-Name": "COM9", "Core-SLM": "", "555 AO-Volts": "0.0000", "555 Shutter-Block voltage": "false", "XYStage-Speed": "1800.0000", "520 AO-MaxVolts": "5.0000", "528 Shutter-SupportsTriggering": "0", "COM1-Description": "Serial port driver (boost:asio)", "PRIME-MetadataEnabled": "No", "520 Shutter-MinVolts": "0.0000", "470 Shutter-Sequenceable": "1", "PRIME-PreampDelay": "0", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-MinVolts": "0.0000", "ChannelIndex": 5, "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Core-AutoShutter": "1", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "Exposure-ms": 300, "PRIME-PP 2 ENABLED": "Yes", "640 Shutter-TriggerSampleRate": "100000", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "470 Shutter-IOChannel": "Dev1/ao10", "405 AO-Demo": "No", "PixelSizeUm": 1.1, "470 AO-Demo": "No", "470 Shutter-MaxVolts": "5.0000", "Vincent-D3-Name": "Vincent-D3", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FileName": "img_000000000_P-DAPI-high_003.tif", "LudlController-Reset": "Operate", "PRIME-Exposure": "300.00", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "445-Name": "DA Shutter", "555 Shutter-Name": "AnalogIO", "520 Shutter-TriggerSampleRate": "100000", "445 Shutter-IOChannel": "Dev1/ao9", "PRIME-TransposeCorrection": "0", "XYStage-StepSize": "0.1000", "445-Description": "DA device that is used as a shutter", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 AO-IOChannel": "Dev1/ao7", "520 Shutter-Name": "AnalogIO", "COM3-DataBits": "8", "640 AO-Volts": "0.0000", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "555 Shutter-IOChannel": "Dev1/ao13", "WaitInterval": null, "520 Shutter-Sequenceable": "1", "YPositionUm": 20690.300000000003, "445-State": "0", "445-DA Device": "445 Shutter", "PRIME-CircularBufferAutoSize": "ON", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "555 AO-TriggerSampleRate": "100000", "445 Shutter-Volts": "0.0000", "XLIGHT Hub-Port": "COM3", "Core-AutoFocus": "", "COM4-Handshaking": "Off", "528 Shutter-MinVolts": "0.0000", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "445 Shutter-Name": "AnalogIO", "528 Shutter-Sequenceable": "1", "XYStage-TransposeMirrorX": "0", "XYStage-TransposeMirrorY": "0", "PRIME-ReadoutRate": "100MHz 16bit", "XYStage-Name": "Stage", "640 AO-SupportsTriggering": "0", "640-Name": "DA Shutter", "640 Shutter-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "640 AO-Block voltage": "false", "445 Shutter-Sequenceable": "1", "470 AO-MaxVolts": "5.0000", "COM4-BaudRate": "9600", "470-Description": "DA device that is used as a shutter", "520 Shutter-Description": "NI DAC", "PRIME-CCDTemperature": "-14.9600", "Time": "2019-12-17 21:59:13 -0500", "640 AO-TriggerSequenceLength": "1000", "470 AO-Name": "AnalogIO", "XLIGHT Disk slider-Label": "Disk Pos 40um", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "Binning": "1x1", "TE2000-ModelType": "1", "COM3-AnswerTimeout": "500.0000", "555 Shutter-SupportsTriggering": "0", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "TE2000-Port": "COM4", "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Description": "NI DAC", "COM4-AnswerTimeout": "500.0000", "COM1-DelayBetweenCharsMs": "0.0000", "FilterBlock-Label": "Confocal", "445 AO-Name": "AnalogIO", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-Timing-ReadoutTimeNs": "24672000.0000", "528 AO-Demo": "No", "520 AO-Sequenceable": "1", "555-Description": "DA device that is used as a shutter", "405-DA Device": "405 Shutter", "405 AO-TriggerSequenceLength": "1000", "640 AO-MinVolts": "0.0000", "640 Shutter-Block voltage": "false", "FrameIndex": 0, "PRIME-Port": "CMOS", "555 AO-MinVolts": "0.0000", "COM3-Description": "Serial port driver (boost:asio)", "528 AO-Description": "NI DAC", "555 AO-IOChannel": "Dev1/ao6", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "555 AO-TriggerInputLine": "/Dev1/PFI0", "PRIME-ActualInterval-ms": "374.3700", "LudlController-Port": "COM9", "445 Shutter-Demo": "No", "PRIME-Trigger-Expose Out-Mux": "1", "SliceIndex": 3, "PRIME-CCDTemperatureSetPoint": "-15.0000", "405 Shutter-MinVolts": "0.0000", "PRIME-ExposeOutMode": "First Row", "AxisPositions": null, "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Volts": "0.0000", "528-Name": "DA Shutter", "555-State": "0", "XLIGHT Dichroic-Label": "QUAD", "445 AO-Sequenceable": "1", "405-Name": "DA Shutter", "405 AO-IOChannel": "Dev1/ao0", "Core-Camera": "PRIME", "Focus-Name": "Focus", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "405 Shutter-IOChannel": "Dev1/ao8", "640 AO-Demo": "No", "640 Shutter-SupportsTriggering": "0", "445 AO-Demo": "No", "555-DA Device": "555 Shutter", "470 Shutter-Description": "NI DAC", "528 AO-Volts": "0.0000", "COM1-DataBits": "8", "Vincent-D3-Address": "x", "520 AO-SupportsTriggering": "0", "405-Description": "DA device that is used as a shutter", "640 Shutter-MinVolts": "0.0000", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "405 AO-Name": "AnalogIO", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "640 AO-TriggerInputLine": "/Dev1/PFI0", "405 AO-TriggerInputLine": "/Dev1/PFI0", "640-Description": "DA device that is used as a shutter", "445 Shutter-TriggerSampleRate": "100000", "470 Shutter-TriggerSequenceLength": "1000", "COM1-StopBits": "1", "520 Shutter-SupportsTriggering": "0", "405-State": "1", "Core-Initialize": "1", "XLIGHT Emission Wheel-State": "7", "520 AO-Description": "NI DAC", "528 AO-TriggerInputLine": "/Dev1/PFI0", "445 Shutter-MaxVolts": "5.0000", "COM9-Handshaking": "Off", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "XLIGHT Spin motor-Label": "On (spinning)", "PRIME-PP 5 ENABLED": "No", "UUID": "2358942c-ab71-49ad-8e6c-410235e35c50", "PRIME-TriggerMode": "Internal Trigger", "COM1-Handshaking": "Off", "PRIME-Timing-PostTriggerDelayNs": "49344000.0000", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "Focus-Position": "16479.8000", "528 Shutter-Block voltage": "false", "Channel": "P-DAPI-high", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "555 Shutter-Demo": "No", "405 Shutter-Demo": "No", "528 AO-Name": "AnalogIO", "520-State": "0", "Core-Galvo": "", "405 Shutter-TriggerSampleRate": "100000", "Focus-FocusStepSize_(nm)": "100", "PRIME-PixelType": "16bit", "COM4-DataBits": "8", "470 AO-IOChannel": "Dev1/ao2", "520 AO-MinVolts": "0.0000", "COM9-Parity": "None", "PRIME-CircularBufferEnabled": "ON", "PRIME-PP 6 Reset": "No", "640 AO-Description": "NI DAC", "Source": "PRIME", "445 AO-MinVolts": "0.0000", "PIEZO-Name": "Stage", "445 AO-Description": "NI DAC", "PRIME-CircularBufferFrameCount": "46", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "520 AO-Volts": "0.0000", "528 Shutter-TriggerSampleRate": "100000", "XPositionUm": 62321, "OpticalPath-State": "4", "PRIME-PP 3 THRESHOLD": "80", "PRIME-PVCAM Version": "3.7.1", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Gain": "1-HDR", "640 Shutter-TriggerSequenceLength": "1000", "528 AO-SupportsTriggering": "0", "445 AO-TriggerInputLine": "/Dev1/PFI0", "OpticalPath-Name": "OpticalPath", "PRIME-PP 4 ENABLED": "Yes", "PRIME-PVCAM Adapter Version": "1.3.29", "COM3-Name": "COM3", "405 AO-MaxVolts": "5.0000", "520 Shutter-Block voltage": "false", "PRIME-Timing-ClearingTimeNs": "49344000.0000", "Core-Focus": "PIEZO", "528 AO-Block voltage": "false", "TE2000-Description": "Nikon TE2000 hub", "528 AO-MinVolts": "0.0000", "COM4-Parity": "None", "Summary": { "Slices": 11, "ChContrastMin": [ 0, 0, 0, 0, 0, 0 ], "PixelAspect": 1, "SlicesFirst": true, "Interval_ms": 0, "Time": "2019-12-17 21:58:39 -0500", "Prefix": "seq_3", "InitialPositionList": [ { "Label": "Pos0", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.435], "Focus": [16480.2], "XYStage": [ 69822.40000000001, 20100.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos1", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.425], "Focus": [16480.3], "XYStage": [ 69004, 20101 ] }, "GridColumnIndex": 0 }, { "Label": "Pos2", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.44], "Focus": [16480.1], "XYStage": [ 63119.5, 20690.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos3", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.405], "Focus": [16480.2], "XYStage": [ 62321, 20690.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos4", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.415], "Focus": [16480], "XYStage": [ 56604.100000000006, 20688.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos5", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.42000000000002], "Focus": [16480.1], "XYStage": [ 56034.200000000004, 20688.800000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos6", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.455], "Focus": [16480], "XYStage": [ 50889.3, 20496.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos7", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.43], "Focus": [16480.2], "XYStage": [ 50101.600000000006, 20496.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos8", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.67000000000002], "Focus": [16479.9], "XYStage": [ 44818.600000000006, 20496.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos9", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.67000000000002], "Focus": [16480], "XYStage": [ 43897.9, 20436.800000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos10", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.675], "Focus": [16479.9], "XYStage": [ 39670.600000000006, 20664.9 ] }, "GridColumnIndex": 0 }, { "Label": "Pos11", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.69], "Focus": [16479.9], "XYStage": [ 38734, 20664.600000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos12", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.695], "Focus": [16480.1], "XYStage": [ 34037.200000000004, 20664.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos13", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.685], "Focus": [16480.1], "XYStage": [ 33152, 20664.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos14", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.69], "Focus": [16480.1], "XYStage": [ 28122.800000000003, 20664.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos15", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.675], "Focus": [16480], "XYStage": [ 27422.9, 20664.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos16", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.64000000000001], "Focus": [16480], "XYStage": [ 23005.2, 20664.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos17", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.69], "Focus": [16479.9], "XYStage": [ 21820.600000000002, 20409.600000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos18", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.41], "Focus": [16480.1], "XYStage": [ 73697.6, -13222.800000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos19", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.455], "Focus": [16480.1], "XYStage": [ 72765.8, -13223 ] }, "GridColumnIndex": 0 }, { "Label": "Pos20", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.4], "Focus": [16480.2], "XYStage": [ 67445.5, -12303.800000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos21", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.415], "Focus": [16480.2], "XYStage": [ 66613.3, -12303.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos22", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.415], "Focus": [16480.1], "XYStage": [ 61819.4, -11936.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos23", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.405], "Focus": [16480.2], "XYStage": [ 61325.700000000004, -11937.1 ] }, "GridColumnIndex": 0 }, { "Label": "Pos24", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.46], "Focus": [16480.2], "XYStage": [ 55377, -10974.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos25", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.445], "Focus": [16480.1], "XYStage": [ 54827, -11214.800000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos26", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.435], "Focus": [16480.2], "XYStage": [ 49937.100000000006, -10919.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos27", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.425], "Focus": [16480.1], "XYStage": [ 48764, -10919.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos28", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.42000000000002], "Focus": [16480.2], "XYStage": [ 43978.4, -10920.400000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos29", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.435], "Focus": [16480.2], "XYStage": [ 43106.3, -10920.400000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos30", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.405], "Focus": [16480.3], "XYStage": [ 38212.4, -10920.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos31", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.445], "Focus": [16480.2], "XYStage": [ 37254.9, -10920.400000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos32", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.44], "Focus": [16480.3], "XYStage": [ 32472.800000000003, -11294.400000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos33", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [166.95000000000002], "Focus": [16480.2], "XYStage": [ 31420.7, -11294.400000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos34", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [166.95000000000002], "Focus": [16480.2], "XYStage": [ 26881.5, -11640 ] }, "GridColumnIndex": 0 }, { "Label": "Pos35", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [166.98], "Focus": [16480.2], "XYStage": [ 25876.100000000002, -11641.1 ] }, "GridColumnIndex": 0 } ], "z-step_um": 10, "GridRow": 0, "BitDepth": 16, "Depth": 2, "Channels": 6, "Source": "Micro-Manager", "ChColors": [ -16711681, -256, -65281, -1, -1, -16776961 ], "MetadataVersion": 10, "KeepShutterOpenChannels": true, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536, 65536 ], "CustomIntervals_ms": [], "Height": 1200, "MicroManagerVersion": "1.4.23 20180219", "GridColumn": 0, "UUID": "8c8f9d20-b128-4577-9f91-89e70f22d319", "PixelType": "GRAY16", "Width": 1200, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\Yan\\20191217-YC113-S5S6", "Frames": 1, "Comment": "", "UserName": "Nikon", "PixelSize_um": 1.1, "ROI": [ 0, 0, 1200, 1200 ], "Date": "2019-12-17", "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC", "P-DAPI-high" ], "KeepShutterOpenSlices": true, "IJType": 1, "ComputerName": "ZadorConfocal2", "Positions": 36, "PositionIndex": 3, "CameraTimeout": 20000, "TimeFirst": false }, "640 AO-Name": "AnalogIO", "Vincent-D3-Command": "Close" }, "FrameKey-0-5-4": { "COM4-StopBits": "1", "528 AO-MaxVolts": "5.0000", "XLIGHT Excitation Wheel-State": "2", "470 Shutter-Name": "AnalogIO", "Core-Shutter": "405", "555 AO-Demo": "No", "XLIGHT Dichroic-State": "2", "XYStage-StepSize-Y": "0.1000", "ZPositionUm": 167.405, "XYStage-StepSize-X": "0.1000", "PRIME-FirmwareVersion": "20.28", "PRIME-ClearCycles": "2", "520-Name": "DA Shutter", "405 Shutter-Name": "AnalogIO", "PIEZO-StepSize": "0.0050", "PRIME-ClearMode": "Pre-Exposure", "470 AO-MinVolts": "0.0000", "405 AO-SupportsTriggering": "1", "CameraChannelIndex": 0, "XYStage-StartSpeed": "500.0000", "COM9-StopBits": "2", "405 AO-Description": "NI DAC", "COM4-DelayBetweenCharsMs": "0.0000", "445 AO-Block voltage": "false", "520 AO-TriggerInputLine": "/Dev1/PFI0", "555 AO-Description": "NI DAC", "520 AO-TriggerSampleRate": "100000", "COM4-Verbose": "1", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM4-Name": "COM4", "405 Shutter-Volts": "5.0000", "Vincent-D3-Port": "COM1", "PRIME-PP 4 MIN ADU AFFECTED": "200", "470 Shutter-MinVolts": "0.0000", "PRIME-PP 3 ENABLED": "Yes", "470 AO-Description": "NI DAC", "PRIME-Description": "PVCAM API device adapter", "520 Shutter-MaxVolts": "5.0000", "520 Shutter-Volts": "0.0000", "528 AO-Sequenceable": "1", "PRIME-Binning": "1x1", "555 Shutter-MaxVolts": "5.0000", "528 Shutter-TriggerSequenceLength": "1000", "405 Shutter-Block voltage": "false", "XLIGHT Spin motor-State": "1", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "Vincent-D3-Time to open (ms)": "35.0000", "Vincent-D3-Time to close (ms)": "35.0000", "445 AO-TriggerSampleRate": "100000", "640 Shutter-IOChannel": "Dev1/ao14", "555 AO-Sequenceable": "1", "520 AO-Name": "AnalogIO", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "470 AO-Block voltage": "false", "COM1-Verbose": "1", "COM9-AnswerTimeout": "10000.0000", "528 Shutter-Name": "AnalogIO", "PRIME-Trigger Timeout (secs)": "10", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "NextFrame": 0, "470 AO-TriggerSequenceLength": "1000", "PRIME-TransposeXY": "0", "445 AO-TriggerSequenceLength": "1000", "COM1-Name": "COM1", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "COM9-DataBits": "8", "COM1-AnswerTimeout": "500.0000", "Camera": "", "TE2000-Version": "V2.50-V2.50-V2.10", "528 Shutter-Volts": "0.0000", "XLIGHT Hub-Name": "XLIGHT Hub", "PIEZO-Description": "Ludl stage driver adapter", "PositionName": "Pos3", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSampleRate": "100000", "PRIME-FanSpeedSetpoint": "High", "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "405 AO-TriggerSampleRate": "100000", "470 Shutter-Block voltage": "false", "470-State": "0", "COM3-Parity": "None", "405 AO-Volts": "4.5000", "PRIME-TransposeMirrorY": "0", "PRIME-ChipName": "GS144BSI", "470 AO-Volts": "0.0000", "PRIME-TransposeMirrorX": "0", "445 Shutter-SupportsTriggering": "0", "470 Shutter-SupportsTriggering": "0", "XLIGHT Hub-Description": "XLIGHT hub", "PRIME-PreampOffLimit": "10000", "470 AO-Sequenceable": "1", "528 Shutter-MaxVolts": "5.0000", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "Core-XYStage": "XYStage", "470 AO-TriggerSampleRate": "100000", "PRIME-Timing-PreTriggerDelayNs": "24672000.0000", "528-DA Device": "528 Shutter", "Focus-Description": "Nikon TE2000 focus adapter", "FilterBlock-State": "5", "520 AO-Demo": "No", "COM3-DelayBetweenCharsMs": "0.0000", "470 Shutter-Volts": "0.0000", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "PRIME-PP 2 THRESHOLD": "125", "PRIME-PP 4 THRESHOLD": "75", "XYStage-Acceleration": "4.0000", "470 Shutter-Demo": "No", "PRIME-Name": "PMPCIECam00", "640-DA Device": "640 Shutter", "405 AO-Block voltage": "false", "ElapsedTime-ms": 166090, "PRIME-Offset": "100", "445 AO-IOChannel": "Dev1/ao1", "640 AO-MaxVolts": "5.0000", "PositionIndex": 3, "PRIME-PP 1 ENABLED": "Yes", "COM1-BaudRate": "9600", "640-State": "0", "COM3-Verbose": "1", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "LudlController-TransmissionDelay": "0", "PRIME-FullWellCapacity": "62000", "PRIME-SMARTStreamingEnabled": "No", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "640 Shutter-Description": "NI DAC", "528-Description": "DA device that is used as a shutter", "COM4-Description": "Serial port driver (boost:asio)", "405 Shutter-Description": "NI DAC", "TE2000-Name": "TE2000", "528 Shutter-Description": "NI DAC", "COM9-Description": "Serial port driver (boost:asio)", "LudlController-Name": "LudlController", "445 Shutter-TriggerSequenceLength": "1000", "PRIME-SerialNumber": "A18B203008", "445 AO-Volts": "0.0000", "PRIME-X-dimension": "1200", "PRIME-ShutterCloseDelay": "0", "XLIGHT Disk slider-State": "2", "Position": "Default", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 AO-IOChannel": "Dev1/ao5", "520 AO-IOChannel": "Dev1/ao4", "528 AO-TriggerSequenceLength": "1000", "405 Shutter-MaxVolts": "5.0000", "470-DA Device": "470 Shutter", "PRIME-FTCapable": "No", "Core-ChannelGroup": "Channels", "555 AO-SupportsTriggering": "0", "PRIME-Y-dimension": "1200", "Frame": 0, "555 Shutter-Sequenceable": "1", "PRIME-Timing-ExposureTimeNs": "9992000.0000", "Core-ImageProcessor": "", "528-State": "0", "520 AO-Block voltage": "false", "XLIGHT Excitation Wheel-Label": "QUAD", "640 Shutter-Demo": "No", "COM3-StopBits": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "520 Shutter-IOChannel": "Dev1/ao11", "Width": 1200, "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-SupportsTriggering": "0", "640 AO-TriggerSampleRate": "100000", "520-Description": "DA device that is used as a shutter", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "405 Shutter-Sequenceable": "1", "405 Shutter-SupportsTriggering": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-TriggerSampleRate": "100000", "640 Shutter-MaxVolts": "5.0000", "555 AO-MaxVolts": "5.0000", "520 AO-TriggerSequenceLength": "1000", "COM3-BaudRate": "9600", "COM9-BaudRate": "9600", "Vincent-D3-Channel #": "Ch. #1", "PRIME-PMode": "Normal", "OpticalPath-Label": "Left(100)", "BitDepth": 16, "555 AO-TriggerSequenceLength": "1000", "445 Shutter-Description": "NI DAC", "405 AO-Sequenceable": "1", "PRIME-AcquisitionMethod": "Callbacks", "555 AO-Name": "AnalogIO", "PRIME-ShutterMode": "Pre-Exposure", "COM3-Handshaking": "Off", "445 AO-SupportsTriggering": "0", "ROI": "0-0-1200-1200", "COM9-Verbose": "1", "PRIME-Color": "OFF", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "PRIME-ShutterOpenDelay": "0", "COM1-Parity": "None", "Slice": 4, "SlicePosition": -10, "COM9-DelayBetweenCharsMs": "0.0000", "555-Name": "DA Shutter", "PIEZO-LudlSingleAxisName": "B", "XLIGHT Emission Wheel-Label": "QUAD", "640 Shutter-Name": "AnalogIO", "640 AO-Sequenceable": "1", "520 Shutter-Demo": "No", "528 Shutter-Demo": "No", "470-Name": "DA Shutter", "520-DA Device": "520 Shutter", "XYStage-Description": "Ludl XY stage driver adapter", "445 Shutter-MinVolts": "0.0000", "528 AO-TriggerSampleRate": "100000", "405 AO-MinVolts": "0.0000", "405 Shutter-TriggerSequenceLength": "1000", "Height": 1200, "555 AO-Block voltage": "false", "640 Shutter-Volts": "0.0000", "PixelType": "GRAY16", "445 Shutter-Block voltage": "false", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "520 Shutter-TriggerSequenceLength": "1000", "COM9-Name": "COM9", "Core-SLM": "", "555 AO-Volts": "0.0000", "555 Shutter-Block voltage": "false", "XYStage-Speed": "1800.0000", "520 AO-MaxVolts": "5.0000", "528 Shutter-SupportsTriggering": "0", "COM1-Description": "Serial port driver (boost:asio)", "PRIME-MetadataEnabled": "No", "520 Shutter-MinVolts": "0.0000", "470 Shutter-Sequenceable": "1", "PRIME-PreampDelay": "0", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-MinVolts": "0.0000", "ChannelIndex": 5, "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Core-AutoShutter": "1", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "Exposure-ms": 300, "PRIME-PP 2 ENABLED": "Yes", "640 Shutter-TriggerSampleRate": "100000", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "470 Shutter-IOChannel": "Dev1/ao10", "405 AO-Demo": "No", "PixelSizeUm": 1.1, "470 AO-Demo": "No", "470 Shutter-MaxVolts": "5.0000", "Vincent-D3-Name": "Vincent-D3", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FileName": "img_000000000_P-DAPI-high_004.tif", "LudlController-Reset": "Operate", "PRIME-Exposure": "300.00", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "445-Name": "DA Shutter", "555 Shutter-Name": "AnalogIO", "520 Shutter-TriggerSampleRate": "100000", "445 Shutter-IOChannel": "Dev1/ao9", "PRIME-TransposeCorrection": "0", "XYStage-StepSize": "0.1000", "445-Description": "DA device that is used as a shutter", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 AO-IOChannel": "Dev1/ao7", "520 Shutter-Name": "AnalogIO", "COM3-DataBits": "8", "640 AO-Volts": "0.0000", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "555 Shutter-IOChannel": "Dev1/ao13", "WaitInterval": null, "520 Shutter-Sequenceable": "1", "YPositionUm": 20690.300000000003, "445-State": "0", "445-DA Device": "445 Shutter", "PRIME-CircularBufferAutoSize": "ON", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "555 AO-TriggerSampleRate": "100000", "445 Shutter-Volts": "0.0000", "XLIGHT Hub-Port": "COM3", "Core-AutoFocus": "", "COM4-Handshaking": "Off", "528 Shutter-MinVolts": "0.0000", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "445 Shutter-Name": "AnalogIO", "528 Shutter-Sequenceable": "1", "XYStage-TransposeMirrorX": "0", "XYStage-TransposeMirrorY": "0", "PRIME-ReadoutRate": "100MHz 16bit", "XYStage-Name": "Stage", "640 AO-SupportsTriggering": "0", "640-Name": "DA Shutter", "640 Shutter-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "640 AO-Block voltage": "false", "445 Shutter-Sequenceable": "1", "470 AO-MaxVolts": "5.0000", "COM4-BaudRate": "9600", "470-Description": "DA device that is used as a shutter", "520 Shutter-Description": "NI DAC", "PRIME-CCDTemperature": "-14.9600", "Time": "2019-12-17 21:59:13 -0500", "640 AO-TriggerSequenceLength": "1000", "470 AO-Name": "AnalogIO", "XLIGHT Disk slider-Label": "Disk Pos 40um", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "Binning": "1x1", "TE2000-ModelType": "1", "COM3-AnswerTimeout": "500.0000", "555 Shutter-SupportsTriggering": "0", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "TE2000-Port": "COM4", "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Description": "NI DAC", "COM4-AnswerTimeout": "500.0000", "COM1-DelayBetweenCharsMs": "0.0000", "FilterBlock-Label": "Confocal", "445 AO-Name": "AnalogIO", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-Timing-ReadoutTimeNs": "24672000.0000", "528 AO-Demo": "No", "520 AO-Sequenceable": "1", "555-Description": "DA device that is used as a shutter", "405-DA Device": "405 Shutter", "405 AO-TriggerSequenceLength": "1000", "640 AO-MinVolts": "0.0000", "640 Shutter-Block voltage": "false", "FrameIndex": 0, "PRIME-Port": "CMOS", "555 AO-MinVolts": "0.0000", "COM3-Description": "Serial port driver (boost:asio)", "528 AO-Description": "NI DAC", "555 AO-IOChannel": "Dev1/ao6", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "555 AO-TriggerInputLine": "/Dev1/PFI0", "PRIME-ActualInterval-ms": "374.3700", "LudlController-Port": "COM9", "445 Shutter-Demo": "No", "PRIME-Trigger-Expose Out-Mux": "1", "SliceIndex": 4, "PRIME-CCDTemperatureSetPoint": "-15.0000", "405 Shutter-MinVolts": "0.0000", "PRIME-ExposeOutMode": "First Row", "AxisPositions": null, "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Volts": "0.0000", "528-Name": "DA Shutter", "555-State": "0", "XLIGHT Dichroic-Label": "QUAD", "445 AO-Sequenceable": "1", "405-Name": "DA Shutter", "405 AO-IOChannel": "Dev1/ao0", "Core-Camera": "PRIME", "Focus-Name": "Focus", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "405 Shutter-IOChannel": "Dev1/ao8", "640 AO-Demo": "No", "640 Shutter-SupportsTriggering": "0", "445 AO-Demo": "No", "555-DA Device": "555 Shutter", "470 Shutter-Description": "NI DAC", "528 AO-Volts": "0.0000", "COM1-DataBits": "8", "Vincent-D3-Address": "x", "520 AO-SupportsTriggering": "0", "405-Description": "DA device that is used as a shutter", "640 Shutter-MinVolts": "0.0000", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "405 AO-Name": "AnalogIO", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "640 AO-TriggerInputLine": "/Dev1/PFI0", "405 AO-TriggerInputLine": "/Dev1/PFI0", "640-Description": "DA device that is used as a shutter", "445 Shutter-TriggerSampleRate": "100000", "470 Shutter-TriggerSequenceLength": "1000", "COM1-StopBits": "1", "520 Shutter-SupportsTriggering": "0", "405-State": "1", "Core-Initialize": "1", "XLIGHT Emission Wheel-State": "7", "520 AO-Description": "NI DAC", "528 AO-TriggerInputLine": "/Dev1/PFI0", "445 Shutter-MaxVolts": "5.0000", "COM9-Handshaking": "Off", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "XLIGHT Spin motor-Label": "On (spinning)", "PRIME-PP 5 ENABLED": "No", "UUID": "c2e5afb7-49ea-41a1-94c1-8f27ecd3a2f3", "PRIME-TriggerMode": "Internal Trigger", "COM1-Handshaking": "Off", "PRIME-Timing-PostTriggerDelayNs": "49344000.0000", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "Focus-Position": "16479.8000", "528 Shutter-Block voltage": "false", "Channel": "P-DAPI-high", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "555 Shutter-Demo": "No", "405 Shutter-Demo": "No", "528 AO-Name": "AnalogIO", "520-State": "0", "Core-Galvo": "", "405 Shutter-TriggerSampleRate": "100000", "Focus-FocusStepSize_(nm)": "100", "PRIME-PixelType": "16bit", "COM4-DataBits": "8", "470 AO-IOChannel": "Dev1/ao2", "520 AO-MinVolts": "0.0000", "COM9-Parity": "None", "PRIME-CircularBufferEnabled": "ON", "PRIME-PP 6 Reset": "No", "640 AO-Description": "NI DAC", "Source": "PRIME", "445 AO-MinVolts": "0.0000", "PIEZO-Name": "Stage", "445 AO-Description": "NI DAC", "PRIME-CircularBufferFrameCount": "46", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "520 AO-Volts": "0.0000", "528 Shutter-TriggerSampleRate": "100000", "XPositionUm": 62321, "OpticalPath-State": "4", "PRIME-PP 3 THRESHOLD": "80", "PRIME-PVCAM Version": "3.7.1", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Gain": "1-HDR", "640 Shutter-TriggerSequenceLength": "1000", "528 AO-SupportsTriggering": "0", "445 AO-TriggerInputLine": "/Dev1/PFI0", "OpticalPath-Name": "OpticalPath", "PRIME-PP 4 ENABLED": "Yes", "PRIME-PVCAM Adapter Version": "1.3.29", "COM3-Name": "COM3", "405 AO-MaxVolts": "5.0000", "520 Shutter-Block voltage": "false", "PRIME-Timing-ClearingTimeNs": "49344000.0000", "Core-Focus": "PIEZO", "528 AO-Block voltage": "false", "TE2000-Description": "Nikon TE2000 hub", "528 AO-MinVolts": "0.0000", "COM4-Parity": "None", "Summary": { "Slices": 11, "ChContrastMin": [ 0, 0, 0, 0, 0, 0 ], "PixelAspect": 1, "SlicesFirst": true, "Interval_ms": 0, "Time": "2019-12-17 21:58:39 -0500", "Prefix": "seq_3", "InitialPositionList": [ { "Label": "Pos0", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.435], "Focus": [16480.2], "XYStage": [ 69822.40000000001, 20100.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos1", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.425], "Focus": [16480.3], "XYStage": [ 69004, 20101 ] }, "GridColumnIndex": 0 }, { "Label": "Pos2", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.44], "Focus": [16480.1], "XYStage": [ 63119.5, 20690.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos3", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.405], "Focus": [16480.2], "XYStage": [ 62321, 20690.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos4", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.415], "Focus": [16480], "XYStage": [ 56604.100000000006, 20688.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos5", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.42000000000002], "Focus": [16480.1], "XYStage": [ 56034.200000000004, 20688.800000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos6", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.455], "Focus": [16480], "XYStage": [ 50889.3, 20496.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos7", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.43], "Focus": [16480.2], "XYStage": [ 50101.600000000006, 20496.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos8", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.67000000000002], "Focus": [16479.9], "XYStage": [ 44818.600000000006, 20496.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos9", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.67000000000002], "Focus": [16480], "XYStage": [ 43897.9, 20436.800000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos10", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.675], "Focus": [16479.9], "XYStage": [ 39670.600000000006, 20664.9 ] }, "GridColumnIndex": 0 }, { "Label": "Pos11", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.69], "Focus": [16479.9], "XYStage": [ 38734, 20664.600000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos12", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.695], "Focus": [16480.1], "XYStage": [ 34037.200000000004, 20664.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos13", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.685], "Focus": [16480.1], "XYStage": [ 33152, 20664.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos14", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.69], "Focus": [16480.1], "XYStage": [ 28122.800000000003, 20664.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos15", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.675], "Focus": [16480], "XYStage": [ 27422.9, 20664.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos16", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.64000000000001], "Focus": [16480], "XYStage": [ 23005.2, 20664.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos17", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.69], "Focus": [16479.9], "XYStage": [ 21820.600000000002, 20409.600000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos18", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.41], "Focus": [16480.1], "XYStage": [ 73697.6, -13222.800000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos19", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.455], "Focus": [16480.1], "XYStage": [ 72765.8, -13223 ] }, "GridColumnIndex": 0 }, { "Label": "Pos20", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.4], "Focus": [16480.2], "XYStage": [ 67445.5, -12303.800000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos21", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.415], "Focus": [16480.2], "XYStage": [ 66613.3, -12303.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos22", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.415], "Focus": [16480.1], "XYStage": [ 61819.4, -11936.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos23", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.405], "Focus": [16480.2], "XYStage": [ 61325.700000000004, -11937.1 ] }, "GridColumnIndex": 0 }, { "Label": "Pos24", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.46], "Focus": [16480.2], "XYStage": [ 55377, -10974.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos25", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.445], "Focus": [16480.1], "XYStage": [ 54827, -11214.800000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos26", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.435], "Focus": [16480.2], "XYStage": [ 49937.100000000006, -10919.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos27", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.425], "Focus": [16480.1], "XYStage": [ 48764, -10919.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos28", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.42000000000002], "Focus": [16480.2], "XYStage": [ 43978.4, -10920.400000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos29", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.435], "Focus": [16480.2], "XYStage": [ 43106.3, -10920.400000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos30", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.405], "Focus": [16480.3], "XYStage": [ 38212.4, -10920.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos31", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.445], "Focus": [16480.2], "XYStage": [ 37254.9, -10920.400000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos32", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.44], "Focus": [16480.3], "XYStage": [ 32472.800000000003, -11294.400000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos33", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [166.95000000000002], "Focus": [16480.2], "XYStage": [ 31420.7, -11294.400000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos34", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [166.95000000000002], "Focus": [16480.2], "XYStage": [ 26881.5, -11640 ] }, "GridColumnIndex": 0 }, { "Label": "Pos35", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [166.98], "Focus": [16480.2], "XYStage": [ 25876.100000000002, -11641.1 ] }, "GridColumnIndex": 0 } ], "z-step_um": 10, "GridRow": 0, "BitDepth": 16, "Depth": 2, "Channels": 6, "Source": "Micro-Manager", "ChColors": [ -16711681, -256, -65281, -1, -1, -16776961 ], "MetadataVersion": 10, "KeepShutterOpenChannels": true, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536, 65536 ], "CustomIntervals_ms": [], "Height": 1200, "MicroManagerVersion": "1.4.23 20180219", "GridColumn": 0, "UUID": "8c8f9d20-b128-4577-9f91-89e70f22d319", "PixelType": "GRAY16", "Width": 1200, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\Yan\\20191217-YC113-S5S6", "Frames": 1, "Comment": "", "UserName": "Nikon", "PixelSize_um": 1.1, "ROI": [ 0, 0, 1200, 1200 ], "Date": "2019-12-17", "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC", "P-DAPI-high" ], "KeepShutterOpenSlices": true, "IJType": 1, "ComputerName": "ZadorConfocal2", "Positions": 36, "PositionIndex": 3, "CameraTimeout": 20000, "TimeFirst": false }, "640 AO-Name": "AnalogIO", "Vincent-D3-Command": "Close" }, "FrameKey-0-5-5": { "COM4-StopBits": "1", "528 AO-MaxVolts": "5.0000", "XLIGHT Excitation Wheel-State": "2", "470 Shutter-Name": "AnalogIO", "Core-Shutter": "405", "555 AO-Demo": "No", "XLIGHT Dichroic-State": "2", "XYStage-StepSize-Y": "0.1000", "ZPositionUm": 177.405, "XYStage-StepSize-X": "0.1000", "PRIME-FirmwareVersion": "20.28", "PRIME-ClearCycles": "2", "520-Name": "DA Shutter", "405 Shutter-Name": "AnalogIO", "PIEZO-StepSize": "0.0050", "PRIME-ClearMode": "Pre-Exposure", "470 AO-MinVolts": "0.0000", "405 AO-SupportsTriggering": "1", "CameraChannelIndex": 0, "XYStage-StartSpeed": "500.0000", "COM9-StopBits": "2", "405 AO-Description": "NI DAC", "COM4-DelayBetweenCharsMs": "0.0000", "445 AO-Block voltage": "false", "520 AO-TriggerInputLine": "/Dev1/PFI0", "555 AO-Description": "NI DAC", "520 AO-TriggerSampleRate": "100000", "COM4-Verbose": "1", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM4-Name": "COM4", "405 Shutter-Volts": "5.0000", "Vincent-D3-Port": "COM1", "PRIME-PP 4 MIN ADU AFFECTED": "200", "470 Shutter-MinVolts": "0.0000", "PRIME-PP 3 ENABLED": "Yes", "470 AO-Description": "NI DAC", "PRIME-Description": "PVCAM API device adapter", "520 Shutter-MaxVolts": "5.0000", "520 Shutter-Volts": "0.0000", "528 AO-Sequenceable": "1", "PRIME-Binning": "1x1", "555 Shutter-MaxVolts": "5.0000", "528 Shutter-TriggerSequenceLength": "1000", "405 Shutter-Block voltage": "false", "XLIGHT Spin motor-State": "1", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "Vincent-D3-Time to open (ms)": "35.0000", "Vincent-D3-Time to close (ms)": "35.0000", "445 AO-TriggerSampleRate": "100000", "640 Shutter-IOChannel": "Dev1/ao14", "555 AO-Sequenceable": "1", "520 AO-Name": "AnalogIO", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "470 AO-Block voltage": "false", "COM1-Verbose": "1", "COM9-AnswerTimeout": "10000.0000", "528 Shutter-Name": "AnalogIO", "PRIME-Trigger Timeout (secs)": "10", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "NextFrame": 0, "470 AO-TriggerSequenceLength": "1000", "PRIME-TransposeXY": "0", "445 AO-TriggerSequenceLength": "1000", "COM1-Name": "COM1", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "COM9-DataBits": "8", "COM1-AnswerTimeout": "500.0000", "Camera": "", "TE2000-Version": "V2.50-V2.50-V2.10", "528 Shutter-Volts": "0.0000", "XLIGHT Hub-Name": "XLIGHT Hub", "PIEZO-Description": "Ludl stage driver adapter", "PositionName": "Pos3", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSampleRate": "100000", "PRIME-FanSpeedSetpoint": "High", "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "405 AO-TriggerSampleRate": "100000", "470 Shutter-Block voltage": "false", "470-State": "0", "COM3-Parity": "None", "405 AO-Volts": "4.5000", "PRIME-TransposeMirrorY": "0", "PRIME-ChipName": "GS144BSI", "470 AO-Volts": "0.0000", "PRIME-TransposeMirrorX": "0", "445 Shutter-SupportsTriggering": "0", "470 Shutter-SupportsTriggering": "0", "XLIGHT Hub-Description": "XLIGHT hub", "PRIME-PreampOffLimit": "10000", "470 AO-Sequenceable": "1", "528 Shutter-MaxVolts": "5.0000", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "Core-XYStage": "XYStage", "470 AO-TriggerSampleRate": "100000", "PRIME-Timing-PreTriggerDelayNs": "24672000.0000", "528-DA Device": "528 Shutter", "Focus-Description": "Nikon TE2000 focus adapter", "FilterBlock-State": "5", "520 AO-Demo": "No", "COM3-DelayBetweenCharsMs": "0.0000", "470 Shutter-Volts": "0.0000", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "PRIME-PP 2 THRESHOLD": "125", "PRIME-PP 4 THRESHOLD": "75", "XYStage-Acceleration": "4.0000", "470 Shutter-Demo": "No", "PRIME-Name": "PMPCIECam00", "640-DA Device": "640 Shutter", "405 AO-Block voltage": "false", "ElapsedTime-ms": 166634, "PRIME-Offset": "100", "445 AO-IOChannel": "Dev1/ao1", "640 AO-MaxVolts": "5.0000", "PositionIndex": 3, "PRIME-PP 1 ENABLED": "Yes", "COM1-BaudRate": "9600", "640-State": "0", "COM3-Verbose": "1", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "LudlController-TransmissionDelay": "0", "PRIME-FullWellCapacity": "62000", "PRIME-SMARTStreamingEnabled": "No", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "640 Shutter-Description": "NI DAC", "528-Description": "DA device that is used as a shutter", "COM4-Description": "Serial port driver (boost:asio)", "405 Shutter-Description": "NI DAC", "TE2000-Name": "TE2000", "528 Shutter-Description": "NI DAC", "COM9-Description": "Serial port driver (boost:asio)", "LudlController-Name": "LudlController", "445 Shutter-TriggerSequenceLength": "1000", "PRIME-SerialNumber": "A18B203008", "445 AO-Volts": "0.0000", "PRIME-X-dimension": "1200", "PRIME-ShutterCloseDelay": "0", "XLIGHT Disk slider-State": "2", "Position": "Default", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 AO-IOChannel": "Dev1/ao5", "520 AO-IOChannel": "Dev1/ao4", "528 AO-TriggerSequenceLength": "1000", "405 Shutter-MaxVolts": "5.0000", "470-DA Device": "470 Shutter", "PRIME-FTCapable": "No", "Core-ChannelGroup": "Channels", "555 AO-SupportsTriggering": "0", "PRIME-Y-dimension": "1200", "Frame": 0, "555 Shutter-Sequenceable": "1", "PRIME-Timing-ExposureTimeNs": "9992000.0000", "Core-ImageProcessor": "", "528-State": "0", "520 AO-Block voltage": "false", "XLIGHT Excitation Wheel-Label": "QUAD", "640 Shutter-Demo": "No", "COM3-StopBits": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "520 Shutter-IOChannel": "Dev1/ao11", "Width": 1200, "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-SupportsTriggering": "0", "640 AO-TriggerSampleRate": "100000", "520-Description": "DA device that is used as a shutter", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "405 Shutter-Sequenceable": "1", "405 Shutter-SupportsTriggering": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-TriggerSampleRate": "100000", "640 Shutter-MaxVolts": "5.0000", "555 AO-MaxVolts": "5.0000", "520 AO-TriggerSequenceLength": "1000", "COM3-BaudRate": "9600", "COM9-BaudRate": "9600", "Vincent-D3-Channel #": "Ch. #1", "PRIME-PMode": "Normal", "OpticalPath-Label": "Left(100)", "BitDepth": 16, "555 AO-TriggerSequenceLength": "1000", "445 Shutter-Description": "NI DAC", "405 AO-Sequenceable": "1", "PRIME-AcquisitionMethod": "Callbacks", "555 AO-Name": "AnalogIO", "PRIME-ShutterMode": "Pre-Exposure", "COM3-Handshaking": "Off", "445 AO-SupportsTriggering": "0", "ROI": "0-0-1200-1200", "COM9-Verbose": "1", "PRIME-Color": "OFF", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "PRIME-ShutterOpenDelay": "0", "COM1-Parity": "None", "Slice": 5, "SlicePosition": 0, "COM9-DelayBetweenCharsMs": "0.0000", "555-Name": "DA Shutter", "PIEZO-LudlSingleAxisName": "B", "XLIGHT Emission Wheel-Label": "QUAD", "640 Shutter-Name": "AnalogIO", "640 AO-Sequenceable": "1", "520 Shutter-Demo": "No", "528 Shutter-Demo": "No", "470-Name": "DA Shutter", "520-DA Device": "520 Shutter", "XYStage-Description": "Ludl XY stage driver adapter", "445 Shutter-MinVolts": "0.0000", "528 AO-TriggerSampleRate": "100000", "405 AO-MinVolts": "0.0000", "405 Shutter-TriggerSequenceLength": "1000", "Height": 1200, "555 AO-Block voltage": "false", "640 Shutter-Volts": "0.0000", "PixelType": "GRAY16", "445 Shutter-Block voltage": "false", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "520 Shutter-TriggerSequenceLength": "1000", "COM9-Name": "COM9", "Core-SLM": "", "555 AO-Volts": "0.0000", "555 Shutter-Block voltage": "false", "XYStage-Speed": "1800.0000", "520 AO-MaxVolts": "5.0000", "528 Shutter-SupportsTriggering": "0", "COM1-Description": "Serial port driver (boost:asio)", "PRIME-MetadataEnabled": "No", "520 Shutter-MinVolts": "0.0000", "470 Shutter-Sequenceable": "1", "PRIME-PreampDelay": "0", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-MinVolts": "0.0000", "ChannelIndex": 5, "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Core-AutoShutter": "1", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "Exposure-ms": 300, "PRIME-PP 2 ENABLED": "Yes", "640 Shutter-TriggerSampleRate": "100000", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "470 Shutter-IOChannel": "Dev1/ao10", "405 AO-Demo": "No", "PixelSizeUm": 1.1, "470 AO-Demo": "No", "470 Shutter-MaxVolts": "5.0000", "Vincent-D3-Name": "Vincent-D3", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FileName": "img_000000000_P-DAPI-high_005.tif", "LudlController-Reset": "Operate", "PRIME-Exposure": "300.00", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "445-Name": "DA Shutter", "555 Shutter-Name": "AnalogIO", "520 Shutter-TriggerSampleRate": "100000", "445 Shutter-IOChannel": "Dev1/ao9", "PRIME-TransposeCorrection": "0", "XYStage-StepSize": "0.1000", "445-Description": "DA device that is used as a shutter", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 AO-IOChannel": "Dev1/ao7", "520 Shutter-Name": "AnalogIO", "COM3-DataBits": "8", "640 AO-Volts": "0.0000", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "555 Shutter-IOChannel": "Dev1/ao13", "WaitInterval": null, "520 Shutter-Sequenceable": "1", "YPositionUm": 20690.300000000003, "445-State": "0", "445-DA Device": "445 Shutter", "PRIME-CircularBufferAutoSize": "ON", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "555 AO-TriggerSampleRate": "100000", "445 Shutter-Volts": "0.0000", "XLIGHT Hub-Port": "COM3", "Core-AutoFocus": "", "COM4-Handshaking": "Off", "528 Shutter-MinVolts": "0.0000", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "445 Shutter-Name": "AnalogIO", "528 Shutter-Sequenceable": "1", "XYStage-TransposeMirrorX": "0", "XYStage-TransposeMirrorY": "0", "PRIME-ReadoutRate": "100MHz 16bit", "XYStage-Name": "Stage", "640 AO-SupportsTriggering": "0", "640-Name": "DA Shutter", "640 Shutter-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "640 AO-Block voltage": "false", "445 Shutter-Sequenceable": "1", "470 AO-MaxVolts": "5.0000", "COM4-BaudRate": "9600", "470-Description": "DA device that is used as a shutter", "520 Shutter-Description": "NI DAC", "PRIME-CCDTemperature": "-14.9600", "Time": "2019-12-17 21:59:14 -0500", "640 AO-TriggerSequenceLength": "1000", "470 AO-Name": "AnalogIO", "XLIGHT Disk slider-Label": "Disk Pos 40um", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "Binning": "1x1", "TE2000-ModelType": "1", "COM3-AnswerTimeout": "500.0000", "555 Shutter-SupportsTriggering": "0", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "TE2000-Port": "COM4", "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Description": "NI DAC", "COM4-AnswerTimeout": "500.0000", "COM1-DelayBetweenCharsMs": "0.0000", "FilterBlock-Label": "Confocal", "445 AO-Name": "AnalogIO", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-Timing-ReadoutTimeNs": "24672000.0000", "528 AO-Demo": "No", "520 AO-Sequenceable": "1", "555-Description": "DA device that is used as a shutter", "405-DA Device": "405 Shutter", "405 AO-TriggerSequenceLength": "1000", "640 AO-MinVolts": "0.0000", "640 Shutter-Block voltage": "false", "FrameIndex": 0, "PRIME-Port": "CMOS", "555 AO-MinVolts": "0.0000", "COM3-Description": "Serial port driver (boost:asio)", "528 AO-Description": "NI DAC", "555 AO-IOChannel": "Dev1/ao6", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "555 AO-TriggerInputLine": "/Dev1/PFI0", "PRIME-ActualInterval-ms": "374.3700", "LudlController-Port": "COM9", "445 Shutter-Demo": "No", "PRIME-Trigger-Expose Out-Mux": "1", "SliceIndex": 5, "PRIME-CCDTemperatureSetPoint": "-15.0000", "405 Shutter-MinVolts": "0.0000", "PRIME-ExposeOutMode": "First Row", "AxisPositions": null, "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Volts": "0.0000", "528-Name": "DA Shutter", "555-State": "0", "XLIGHT Dichroic-Label": "QUAD", "445 AO-Sequenceable": "1", "405-Name": "DA Shutter", "405 AO-IOChannel": "Dev1/ao0", "Core-Camera": "PRIME", "Focus-Name": "Focus", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "405 Shutter-IOChannel": "Dev1/ao8", "640 AO-Demo": "No", "640 Shutter-SupportsTriggering": "0", "445 AO-Demo": "No", "555-DA Device": "555 Shutter", "470 Shutter-Description": "NI DAC", "528 AO-Volts": "0.0000", "COM1-DataBits": "8", "Vincent-D3-Address": "x", "520 AO-SupportsTriggering": "0", "405-Description": "DA device that is used as a shutter", "640 Shutter-MinVolts": "0.0000", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "405 AO-Name": "AnalogIO", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "640 AO-TriggerInputLine": "/Dev1/PFI0", "405 AO-TriggerInputLine": "/Dev1/PFI0", "640-Description": "DA device that is used as a shutter", "445 Shutter-TriggerSampleRate": "100000", "470 Shutter-TriggerSequenceLength": "1000", "COM1-StopBits": "1", "520 Shutter-SupportsTriggering": "0", "405-State": "1", "Core-Initialize": "1", "XLIGHT Emission Wheel-State": "7", "520 AO-Description": "NI DAC", "528 AO-TriggerInputLine": "/Dev1/PFI0", "445 Shutter-MaxVolts": "5.0000", "COM9-Handshaking": "Off", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "XLIGHT Spin motor-Label": "On (spinning)", "PRIME-PP 5 ENABLED": "No", "UUID": "8e454719-e9d4-43ac-b837-dafccdb2577f", "PRIME-TriggerMode": "Internal Trigger", "COM1-Handshaking": "Off", "PRIME-Timing-PostTriggerDelayNs": "49344000.0000", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "Focus-Position": "16479.8000", "528 Shutter-Block voltage": "false", "Channel": "P-DAPI-high", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "555 Shutter-Demo": "No", "405 Shutter-Demo": "No", "528 AO-Name": "AnalogIO", "520-State": "0", "Core-Galvo": "", "405 Shutter-TriggerSampleRate": "100000", "Focus-FocusStepSize_(nm)": "100", "PRIME-PixelType": "16bit", "COM4-DataBits": "8", "470 AO-IOChannel": "Dev1/ao2", "520 AO-MinVolts": "0.0000", "COM9-Parity": "None", "PRIME-CircularBufferEnabled": "ON", "PRIME-PP 6 Reset": "No", "640 AO-Description": "NI DAC", "Source": "PRIME", "445 AO-MinVolts": "0.0000", "PIEZO-Name": "Stage", "445 AO-Description": "NI DAC", "PRIME-CircularBufferFrameCount": "46", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "520 AO-Volts": "0.0000", "528 Shutter-TriggerSampleRate": "100000", "XPositionUm": 62321, "OpticalPath-State": "4", "PRIME-PP 3 THRESHOLD": "80", "PRIME-PVCAM Version": "3.7.1", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Gain": "1-HDR", "640 Shutter-TriggerSequenceLength": "1000", "528 AO-SupportsTriggering": "0", "445 AO-TriggerInputLine": "/Dev1/PFI0", "OpticalPath-Name": "OpticalPath", "PRIME-PP 4 ENABLED": "Yes", "PRIME-PVCAM Adapter Version": "1.3.29", "COM3-Name": "COM3", "405 AO-MaxVolts": "5.0000", "520 Shutter-Block voltage": "false", "PRIME-Timing-ClearingTimeNs": "49344000.0000", "Core-Focus": "PIEZO", "528 AO-Block voltage": "false", "TE2000-Description": "Nikon TE2000 hub", "528 AO-MinVolts": "0.0000", "COM4-Parity": "None", "Summary": { "Slices": 11, "ChContrastMin": [ 0, 0, 0, 0, 0, 0 ], "PixelAspect": 1, "SlicesFirst": true, "Interval_ms": 0, "Time": "2019-12-17 21:58:39 -0500", "Prefix": "seq_3", "InitialPositionList": [ { "Label": "Pos0", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.435], "Focus": [16480.2], "XYStage": [ 69822.40000000001, 20100.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos1", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.425], "Focus": [16480.3], "XYStage": [ 69004, 20101 ] }, "GridColumnIndex": 0 }, { "Label": "Pos2", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.44], "Focus": [16480.1], "XYStage": [ 63119.5, 20690.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos3", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.405], "Focus": [16480.2], "XYStage": [ 62321, 20690.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos4", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.415], "Focus": [16480], "XYStage": [ 56604.100000000006, 20688.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos5", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.42000000000002], "Focus": [16480.1], "XYStage": [ 56034.200000000004, 20688.800000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos6", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.455], "Focus": [16480], "XYStage": [ 50889.3, 20496.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos7", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.43], "Focus": [16480.2], "XYStage": [ 50101.600000000006, 20496.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos8", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.67000000000002], "Focus": [16479.9], "XYStage": [ 44818.600000000006, 20496.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos9", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.67000000000002], "Focus": [16480], "XYStage": [ 43897.9, 20436.800000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos10", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.675], "Focus": [16479.9], "XYStage": [ 39670.600000000006, 20664.9 ] }, "GridColumnIndex": 0 }, { "Label": "Pos11", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.69], "Focus": [16479.9], "XYStage": [ 38734, 20664.600000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos12", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.695], "Focus": [16480.1], "XYStage": [ 34037.200000000004, 20664.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos13", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.685], "Focus": [16480.1], "XYStage": [ 33152, 20664.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos14", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.69], "Focus": [16480.1], "XYStage": [ 28122.800000000003, 20664.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos15", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.675], "Focus": [16480], "XYStage": [ 27422.9, 20664.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos16", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.64000000000001], "Focus": [16480], "XYStage": [ 23005.2, 20664.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos17", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.69], "Focus": [16479.9], "XYStage": [ 21820.600000000002, 20409.600000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos18", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.41], "Focus": [16480.1], "XYStage": [ 73697.6, -13222.800000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos19", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.455], "Focus": [16480.1], "XYStage": [ 72765.8, -13223 ] }, "GridColumnIndex": 0 }, { "Label": "Pos20", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.4], "Focus": [16480.2], "XYStage": [ 67445.5, -12303.800000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos21", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.415], "Focus": [16480.2], "XYStage": [ 66613.3, -12303.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos22", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.415], "Focus": [16480.1], "XYStage": [ 61819.4, -11936.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos23", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.405], "Focus": [16480.2], "XYStage": [ 61325.700000000004, -11937.1 ] }, "GridColumnIndex": 0 }, { "Label": "Pos24", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.46], "Focus": [16480.2], "XYStage": [ 55377, -10974.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos25", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.445], "Focus": [16480.1], "XYStage": [ 54827, -11214.800000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos26", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.435], "Focus": [16480.2], "XYStage": [ 49937.100000000006, -10919.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos27", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.425], "Focus": [16480.1], "XYStage": [ 48764, -10919.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos28", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.42000000000002], "Focus": [16480.2], "XYStage": [ 43978.4, -10920.400000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos29", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.435], "Focus": [16480.2], "XYStage": [ 43106.3, -10920.400000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos30", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.405], "Focus": [16480.3], "XYStage": [ 38212.4, -10920.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos31", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.445], "Focus": [16480.2], "XYStage": [ 37254.9, -10920.400000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos32", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.44], "Focus": [16480.3], "XYStage": [ 32472.800000000003, -11294.400000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos33", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [166.95000000000002], "Focus": [16480.2], "XYStage": [ 31420.7, -11294.400000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos34", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [166.95000000000002], "Focus": [16480.2], "XYStage": [ 26881.5, -11640 ] }, "GridColumnIndex": 0 }, { "Label": "Pos35", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [166.98], "Focus": [16480.2], "XYStage": [ 25876.100000000002, -11641.1 ] }, "GridColumnIndex": 0 } ], "z-step_um": 10, "GridRow": 0, "BitDepth": 16, "Depth": 2, "Channels": 6, "Source": "Micro-Manager", "ChColors": [ -16711681, -256, -65281, -1, -1, -16776961 ], "MetadataVersion": 10, "KeepShutterOpenChannels": true, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536, 65536 ], "CustomIntervals_ms": [], "Height": 1200, "MicroManagerVersion": "1.4.23 20180219", "GridColumn": 0, "UUID": "8c8f9d20-b128-4577-9f91-89e70f22d319", "PixelType": "GRAY16", "Width": 1200, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\Yan\\20191217-YC113-S5S6", "Frames": 1, "Comment": "", "UserName": "Nikon", "PixelSize_um": 1.1, "ROI": [ 0, 0, 1200, 1200 ], "Date": "2019-12-17", "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC", "P-DAPI-high" ], "KeepShutterOpenSlices": true, "IJType": 1, "ComputerName": "ZadorConfocal2", "Positions": 36, "PositionIndex": 3, "CameraTimeout": 20000, "TimeFirst": false }, "640 AO-Name": "AnalogIO", "Vincent-D3-Command": "Close" }, "FrameKey-0-5-6": { "COM4-StopBits": "1", "528 AO-MaxVolts": "5.0000", "XLIGHT Excitation Wheel-State": "2", "470 Shutter-Name": "AnalogIO", "Core-Shutter": "405", "555 AO-Demo": "No", "XLIGHT Dichroic-State": "2", "XYStage-StepSize-Y": "0.1000", "ZPositionUm": 187.405, "XYStage-StepSize-X": "0.1000", "PRIME-FirmwareVersion": "20.28", "PRIME-ClearCycles": "2", "520-Name": "DA Shutter", "405 Shutter-Name": "AnalogIO", "PIEZO-StepSize": "0.0050", "PRIME-ClearMode": "Pre-Exposure", "470 AO-MinVolts": "0.0000", "405 AO-SupportsTriggering": "1", "CameraChannelIndex": 0, "XYStage-StartSpeed": "500.0000", "COM9-StopBits": "2", "405 AO-Description": "NI DAC", "COM4-DelayBetweenCharsMs": "0.0000", "445 AO-Block voltage": "false", "520 AO-TriggerInputLine": "/Dev1/PFI0", "555 AO-Description": "NI DAC", "520 AO-TriggerSampleRate": "100000", "COM4-Verbose": "1", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM4-Name": "COM4", "405 Shutter-Volts": "5.0000", "Vincent-D3-Port": "COM1", "PRIME-PP 4 MIN ADU AFFECTED": "200", "470 Shutter-MinVolts": "0.0000", "PRIME-PP 3 ENABLED": "Yes", "470 AO-Description": "NI DAC", "PRIME-Description": "PVCAM API device adapter", "520 Shutter-MaxVolts": "5.0000", "520 Shutter-Volts": "0.0000", "528 AO-Sequenceable": "1", "PRIME-Binning": "1x1", "555 Shutter-MaxVolts": "5.0000", "528 Shutter-TriggerSequenceLength": "1000", "405 Shutter-Block voltage": "false", "XLIGHT Spin motor-State": "1", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "Vincent-D3-Time to open (ms)": "35.0000", "Vincent-D3-Time to close (ms)": "35.0000", "445 AO-TriggerSampleRate": "100000", "640 Shutter-IOChannel": "Dev1/ao14", "555 AO-Sequenceable": "1", "520 AO-Name": "AnalogIO", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "470 AO-Block voltage": "false", "COM1-Verbose": "1", "COM9-AnswerTimeout": "10000.0000", "528 Shutter-Name": "AnalogIO", "PRIME-Trigger Timeout (secs)": "10", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "NextFrame": 0, "470 AO-TriggerSequenceLength": "1000", "PRIME-TransposeXY": "0", "445 AO-TriggerSequenceLength": "1000", "COM1-Name": "COM1", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "COM9-DataBits": "8", "COM1-AnswerTimeout": "500.0000", "Camera": "", "TE2000-Version": "V2.50-V2.50-V2.10", "528 Shutter-Volts": "0.0000", "XLIGHT Hub-Name": "XLIGHT Hub", "PIEZO-Description": "Ludl stage driver adapter", "PositionName": "Pos3", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSampleRate": "100000", "PRIME-FanSpeedSetpoint": "High", "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "405 AO-TriggerSampleRate": "100000", "470 Shutter-Block voltage": "false", "470-State": "0", "COM3-Parity": "None", "405 AO-Volts": "4.5000", "PRIME-TransposeMirrorY": "0", "PRIME-ChipName": "GS144BSI", "470 AO-Volts": "0.0000", "PRIME-TransposeMirrorX": "0", "445 Shutter-SupportsTriggering": "0", "470 Shutter-SupportsTriggering": "0", "XLIGHT Hub-Description": "XLIGHT hub", "PRIME-PreampOffLimit": "10000", "470 AO-Sequenceable": "1", "528 Shutter-MaxVolts": "5.0000", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "Core-XYStage": "XYStage", "470 AO-TriggerSampleRate": "100000", "PRIME-Timing-PreTriggerDelayNs": "24672000.0000", "528-DA Device": "528 Shutter", "Focus-Description": "Nikon TE2000 focus adapter", "FilterBlock-State": "5", "520 AO-Demo": "No", "COM3-DelayBetweenCharsMs": "0.0000", "470 Shutter-Volts": "0.0000", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "PRIME-PP 2 THRESHOLD": "125", "PRIME-PP 4 THRESHOLD": "75", "XYStage-Acceleration": "4.0000", "470 Shutter-Demo": "No", "PRIME-Name": "PMPCIECam00", "640-DA Device": "640 Shutter", "405 AO-Block voltage": "false", "ElapsedTime-ms": 167177, "PRIME-Offset": "100", "445 AO-IOChannel": "Dev1/ao1", "640 AO-MaxVolts": "5.0000", "PositionIndex": 3, "PRIME-PP 1 ENABLED": "Yes", "COM1-BaudRate": "9600", "640-State": "0", "COM3-Verbose": "1", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "LudlController-TransmissionDelay": "0", "PRIME-FullWellCapacity": "62000", "PRIME-SMARTStreamingEnabled": "No", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "640 Shutter-Description": "NI DAC", "528-Description": "DA device that is used as a shutter", "COM4-Description": "Serial port driver (boost:asio)", "405 Shutter-Description": "NI DAC", "TE2000-Name": "TE2000", "528 Shutter-Description": "NI DAC", "COM9-Description": "Serial port driver (boost:asio)", "LudlController-Name": "LudlController", "445 Shutter-TriggerSequenceLength": "1000", "PRIME-SerialNumber": "A18B203008", "445 AO-Volts": "0.0000", "PRIME-X-dimension": "1200", "PRIME-ShutterCloseDelay": "0", "XLIGHT Disk slider-State": "2", "Position": "Default", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 AO-IOChannel": "Dev1/ao5", "520 AO-IOChannel": "Dev1/ao4", "528 AO-TriggerSequenceLength": "1000", "405 Shutter-MaxVolts": "5.0000", "470-DA Device": "470 Shutter", "PRIME-FTCapable": "No", "Core-ChannelGroup": "Channels", "555 AO-SupportsTriggering": "0", "PRIME-Y-dimension": "1200", "Frame": 0, "555 Shutter-Sequenceable": "1", "PRIME-Timing-ExposureTimeNs": "9992000.0000", "Core-ImageProcessor": "", "528-State": "0", "520 AO-Block voltage": "false", "XLIGHT Excitation Wheel-Label": "QUAD", "640 Shutter-Demo": "No", "COM3-StopBits": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "520 Shutter-IOChannel": "Dev1/ao11", "Width": 1200, "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-SupportsTriggering": "0", "640 AO-TriggerSampleRate": "100000", "520-Description": "DA device that is used as a shutter", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "405 Shutter-Sequenceable": "1", "405 Shutter-SupportsTriggering": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-TriggerSampleRate": "100000", "640 Shutter-MaxVolts": "5.0000", "555 AO-MaxVolts": "5.0000", "520 AO-TriggerSequenceLength": "1000", "COM3-BaudRate": "9600", "COM9-BaudRate": "9600", "Vincent-D3-Channel #": "Ch. #1", "PRIME-PMode": "Normal", "OpticalPath-Label": "Left(100)", "BitDepth": 16, "555 AO-TriggerSequenceLength": "1000", "445 Shutter-Description": "NI DAC", "405 AO-Sequenceable": "1", "PRIME-AcquisitionMethod": "Callbacks", "555 AO-Name": "AnalogIO", "PRIME-ShutterMode": "Pre-Exposure", "COM3-Handshaking": "Off", "445 AO-SupportsTriggering": "0", "ROI": "0-0-1200-1200", "COM9-Verbose": "1", "PRIME-Color": "OFF", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "PRIME-ShutterOpenDelay": "0", "COM1-Parity": "None", "Slice": 6, "SlicePosition": 10, "COM9-DelayBetweenCharsMs": "0.0000", "555-Name": "DA Shutter", "PIEZO-LudlSingleAxisName": "B", "XLIGHT Emission Wheel-Label": "QUAD", "640 Shutter-Name": "AnalogIO", "640 AO-Sequenceable": "1", "520 Shutter-Demo": "No", "528 Shutter-Demo": "No", "470-Name": "DA Shutter", "520-DA Device": "520 Shutter", "XYStage-Description": "Ludl XY stage driver adapter", "445 Shutter-MinVolts": "0.0000", "528 AO-TriggerSampleRate": "100000", "405 AO-MinVolts": "0.0000", "405 Shutter-TriggerSequenceLength": "1000", "Height": 1200, "555 AO-Block voltage": "false", "640 Shutter-Volts": "0.0000", "PixelType": "GRAY16", "445 Shutter-Block voltage": "false", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "520 Shutter-TriggerSequenceLength": "1000", "COM9-Name": "COM9", "Core-SLM": "", "555 AO-Volts": "0.0000", "555 Shutter-Block voltage": "false", "XYStage-Speed": "1800.0000", "520 AO-MaxVolts": "5.0000", "528 Shutter-SupportsTriggering": "0", "COM1-Description": "Serial port driver (boost:asio)", "PRIME-MetadataEnabled": "No", "520 Shutter-MinVolts": "0.0000", "470 Shutter-Sequenceable": "1", "PRIME-PreampDelay": "0", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-MinVolts": "0.0000", "ChannelIndex": 5, "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Core-AutoShutter": "1", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "Exposure-ms": 300, "PRIME-PP 2 ENABLED": "Yes", "640 Shutter-TriggerSampleRate": "100000", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "470 Shutter-IOChannel": "Dev1/ao10", "405 AO-Demo": "No", "PixelSizeUm": 1.1, "470 AO-Demo": "No", "470 Shutter-MaxVolts": "5.0000", "Vincent-D3-Name": "Vincent-D3", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FileName": "img_000000000_P-DAPI-high_006.tif", "LudlController-Reset": "Operate", "PRIME-Exposure": "300.00", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "445-Name": "DA Shutter", "555 Shutter-Name": "AnalogIO", "520 Shutter-TriggerSampleRate": "100000", "445 Shutter-IOChannel": "Dev1/ao9", "PRIME-TransposeCorrection": "0", "XYStage-StepSize": "0.1000", "445-Description": "DA device that is used as a shutter", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 AO-IOChannel": "Dev1/ao7", "520 Shutter-Name": "AnalogIO", "COM3-DataBits": "8", "640 AO-Volts": "0.0000", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "555 Shutter-IOChannel": "Dev1/ao13", "WaitInterval": null, "520 Shutter-Sequenceable": "1", "YPositionUm": 20690.300000000003, "445-State": "0", "445-DA Device": "445 Shutter", "PRIME-CircularBufferAutoSize": "ON", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "555 AO-TriggerSampleRate": "100000", "445 Shutter-Volts": "0.0000", "XLIGHT Hub-Port": "COM3", "Core-AutoFocus": "", "COM4-Handshaking": "Off", "528 Shutter-MinVolts": "0.0000", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "445 Shutter-Name": "AnalogIO", "528 Shutter-Sequenceable": "1", "XYStage-TransposeMirrorX": "0", "XYStage-TransposeMirrorY": "0", "PRIME-ReadoutRate": "100MHz 16bit", "XYStage-Name": "Stage", "640 AO-SupportsTriggering": "0", "640-Name": "DA Shutter", "640 Shutter-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "640 AO-Block voltage": "false", "445 Shutter-Sequenceable": "1", "470 AO-MaxVolts": "5.0000", "COM4-BaudRate": "9600", "470-Description": "DA device that is used as a shutter", "520 Shutter-Description": "NI DAC", "PRIME-CCDTemperature": "-14.9600", "Time": "2019-12-17 21:59:14 -0500", "640 AO-TriggerSequenceLength": "1000", "470 AO-Name": "AnalogIO", "XLIGHT Disk slider-Label": "Disk Pos 40um", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "Binning": "1x1", "TE2000-ModelType": "1", "COM3-AnswerTimeout": "500.0000", "555 Shutter-SupportsTriggering": "0", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "TE2000-Port": "COM4", "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Description": "NI DAC", "COM4-AnswerTimeout": "500.0000", "COM1-DelayBetweenCharsMs": "0.0000", "FilterBlock-Label": "Confocal", "445 AO-Name": "AnalogIO", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-Timing-ReadoutTimeNs": "24672000.0000", "528 AO-Demo": "No", "520 AO-Sequenceable": "1", "555-Description": "DA device that is used as a shutter", "405-DA Device": "405 Shutter", "405 AO-TriggerSequenceLength": "1000", "640 AO-MinVolts": "0.0000", "640 Shutter-Block voltage": "false", "FrameIndex": 0, "PRIME-Port": "CMOS", "555 AO-MinVolts": "0.0000", "COM3-Description": "Serial port driver (boost:asio)", "528 AO-Description": "NI DAC", "555 AO-IOChannel": "Dev1/ao6", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "555 AO-TriggerInputLine": "/Dev1/PFI0", "PRIME-ActualInterval-ms": "374.3700", "LudlController-Port": "COM9", "445 Shutter-Demo": "No", "PRIME-Trigger-Expose Out-Mux": "1", "SliceIndex": 6, "PRIME-CCDTemperatureSetPoint": "-15.0000", "405 Shutter-MinVolts": "0.0000", "PRIME-ExposeOutMode": "First Row", "AxisPositions": null, "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Volts": "0.0000", "528-Name": "DA Shutter", "555-State": "0", "XLIGHT Dichroic-Label": "QUAD", "445 AO-Sequenceable": "1", "405-Name": "DA Shutter", "405 AO-IOChannel": "Dev1/ao0", "Core-Camera": "PRIME", "Focus-Name": "Focus", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "405 Shutter-IOChannel": "Dev1/ao8", "640 AO-Demo": "No", "640 Shutter-SupportsTriggering": "0", "445 AO-Demo": "No", "555-DA Device": "555 Shutter", "470 Shutter-Description": "NI DAC", "528 AO-Volts": "0.0000", "COM1-DataBits": "8", "Vincent-D3-Address": "x", "520 AO-SupportsTriggering": "0", "405-Description": "DA device that is used as a shutter", "640 Shutter-MinVolts": "0.0000", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "405 AO-Name": "AnalogIO", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "640 AO-TriggerInputLine": "/Dev1/PFI0", "405 AO-TriggerInputLine": "/Dev1/PFI0", "640-Description": "DA device that is used as a shutter", "445 Shutter-TriggerSampleRate": "100000", "470 Shutter-TriggerSequenceLength": "1000", "COM1-StopBits": "1", "520 Shutter-SupportsTriggering": "0", "405-State": "1", "Core-Initialize": "1", "XLIGHT Emission Wheel-State": "7", "520 AO-Description": "NI DAC", "528 AO-TriggerInputLine": "/Dev1/PFI0", "445 Shutter-MaxVolts": "5.0000", "COM9-Handshaking": "Off", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "XLIGHT Spin motor-Label": "On (spinning)", "PRIME-PP 5 ENABLED": "No", "UUID": "5fd669bf-d939-496b-87b0-e4055d57fcf9", "PRIME-TriggerMode": "Internal Trigger", "COM1-Handshaking": "Off", "PRIME-Timing-PostTriggerDelayNs": "49344000.0000", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "Focus-Position": "16479.8000", "528 Shutter-Block voltage": "false", "Channel": "P-DAPI-high", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "555 Shutter-Demo": "No", "405 Shutter-Demo": "No", "528 AO-Name": "AnalogIO", "520-State": "0", "Core-Galvo": "", "405 Shutter-TriggerSampleRate": "100000", "Focus-FocusStepSize_(nm)": "100", "PRIME-PixelType": "16bit", "COM4-DataBits": "8", "470 AO-IOChannel": "Dev1/ao2", "520 AO-MinVolts": "0.0000", "COM9-Parity": "None", "PRIME-CircularBufferEnabled": "ON", "PRIME-PP 6 Reset": "No", "640 AO-Description": "NI DAC", "Source": "PRIME", "445 AO-MinVolts": "0.0000", "PIEZO-Name": "Stage", "445 AO-Description": "NI DAC", "PRIME-CircularBufferFrameCount": "46", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "520 AO-Volts": "0.0000", "528 Shutter-TriggerSampleRate": "100000", "XPositionUm": 62321, "OpticalPath-State": "4", "PRIME-PP 3 THRESHOLD": "80", "PRIME-PVCAM Version": "3.7.1", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Gain": "1-HDR", "640 Shutter-TriggerSequenceLength": "1000", "528 AO-SupportsTriggering": "0", "445 AO-TriggerInputLine": "/Dev1/PFI0", "OpticalPath-Name": "OpticalPath", "PRIME-PP 4 ENABLED": "Yes", "PRIME-PVCAM Adapter Version": "1.3.29", "COM3-Name": "COM3", "405 AO-MaxVolts": "5.0000", "520 Shutter-Block voltage": "false", "PRIME-Timing-ClearingTimeNs": "49344000.0000", "Core-Focus": "PIEZO", "528 AO-Block voltage": "false", "TE2000-Description": "Nikon TE2000 hub", "528 AO-MinVolts": "0.0000", "COM4-Parity": "None", "Summary": { "Slices": 11, "ChContrastMin": [ 0, 0, 0, 0, 0, 0 ], "PixelAspect": 1, "SlicesFirst": true, "Interval_ms": 0, "Time": "2019-12-17 21:58:39 -0500", "Prefix": "seq_3", "InitialPositionList": [ { "Label": "Pos0", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.435], "Focus": [16480.2], "XYStage": [ 69822.40000000001, 20100.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos1", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.425], "Focus": [16480.3], "XYStage": [ 69004, 20101 ] }, "GridColumnIndex": 0 }, { "Label": "Pos2", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.44], "Focus": [16480.1], "XYStage": [ 63119.5, 20690.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos3", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.405], "Focus": [16480.2], "XYStage": [ 62321, 20690.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos4", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.415], "Focus": [16480], "XYStage": [ 56604.100000000006, 20688.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos5", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.42000000000002], "Focus": [16480.1], "XYStage": [ 56034.200000000004, 20688.800000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos6", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.455], "Focus": [16480], "XYStage": [ 50889.3, 20496.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos7", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.43], "Focus": [16480.2], "XYStage": [ 50101.600000000006, 20496.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos8", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.67000000000002], "Focus": [16479.9], "XYStage": [ 44818.600000000006, 20496.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos9", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.67000000000002], "Focus": [16480], "XYStage": [ 43897.9, 20436.800000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos10", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.675], "Focus": [16479.9], "XYStage": [ 39670.600000000006, 20664.9 ] }, "GridColumnIndex": 0 }, { "Label": "Pos11", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.69], "Focus": [16479.9], "XYStage": [ 38734, 20664.600000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos12", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.695], "Focus": [16480.1], "XYStage": [ 34037.200000000004, 20664.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos13", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.685], "Focus": [16480.1], "XYStage": [ 33152, 20664.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos14", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.69], "Focus": [16480.1], "XYStage": [ 28122.800000000003, 20664.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos15", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.675], "Focus": [16480], "XYStage": [ 27422.9, 20664.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos16", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.64000000000001], "Focus": [16480], "XYStage": [ 23005.2, 20664.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos17", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.69], "Focus": [16479.9], "XYStage": [ 21820.600000000002, 20409.600000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos18", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.41], "Focus": [16480.1], "XYStage": [ 73697.6, -13222.800000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos19", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.455], "Focus": [16480.1], "XYStage": [ 72765.8, -13223 ] }, "GridColumnIndex": 0 }, { "Label": "Pos20", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.4], "Focus": [16480.2], "XYStage": [ 67445.5, -12303.800000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos21", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.415], "Focus": [16480.2], "XYStage": [ 66613.3, -12303.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos22", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.415], "Focus": [16480.1], "XYStage": [ 61819.4, -11936.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos23", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.405], "Focus": [16480.2], "XYStage": [ 61325.700000000004, -11937.1 ] }, "GridColumnIndex": 0 }, { "Label": "Pos24", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.46], "Focus": [16480.2], "XYStage": [ 55377, -10974.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos25", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.445], "Focus": [16480.1], "XYStage": [ 54827, -11214.800000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos26", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.435], "Focus": [16480.2], "XYStage": [ 49937.100000000006, -10919.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos27", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.425], "Focus": [16480.1], "XYStage": [ 48764, -10919.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos28", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.42000000000002], "Focus": [16480.2], "XYStage": [ 43978.4, -10920.400000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos29", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.435], "Focus": [16480.2], "XYStage": [ 43106.3, -10920.400000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos30", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.405], "Focus": [16480.3], "XYStage": [ 38212.4, -10920.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos31", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.445], "Focus": [16480.2], "XYStage": [ 37254.9, -10920.400000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos32", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.44], "Focus": [16480.3], "XYStage": [ 32472.800000000003, -11294.400000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos33", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [166.95000000000002], "Focus": [16480.2], "XYStage": [ 31420.7, -11294.400000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos34", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [166.95000000000002], "Focus": [16480.2], "XYStage": [ 26881.5, -11640 ] }, "GridColumnIndex": 0 }, { "Label": "Pos35", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [166.98], "Focus": [16480.2], "XYStage": [ 25876.100000000002, -11641.1 ] }, "GridColumnIndex": 0 } ], "z-step_um": 10, "GridRow": 0, "BitDepth": 16, "Depth": 2, "Channels": 6, "Source": "Micro-Manager", "ChColors": [ -16711681, -256, -65281, -1, -1, -16776961 ], "MetadataVersion": 10, "KeepShutterOpenChannels": true, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536, 65536 ], "CustomIntervals_ms": [], "Height": 1200, "MicroManagerVersion": "1.4.23 20180219", "GridColumn": 0, "UUID": "8c8f9d20-b128-4577-9f91-89e70f22d319", "PixelType": "GRAY16", "Width": 1200, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\Yan\\20191217-YC113-S5S6", "Frames": 1, "Comment": "", "UserName": "Nikon", "PixelSize_um": 1.1, "ROI": [ 0, 0, 1200, 1200 ], "Date": "2019-12-17", "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC", "P-DAPI-high" ], "KeepShutterOpenSlices": true, "IJType": 1, "ComputerName": "ZadorConfocal2", "Positions": 36, "PositionIndex": 3, "CameraTimeout": 20000, "TimeFirst": false }, "640 AO-Name": "AnalogIO", "Vincent-D3-Command": "Close" }, "FrameKey-0-5-7": { "COM4-StopBits": "1", "528 AO-MaxVolts": "5.0000", "XLIGHT Excitation Wheel-State": "2", "470 Shutter-Name": "AnalogIO", "Core-Shutter": "405", "555 AO-Demo": "No", "XLIGHT Dichroic-State": "2", "XYStage-StepSize-Y": "0.1000", "ZPositionUm": 197.405, "XYStage-StepSize-X": "0.1000", "PRIME-FirmwareVersion": "20.28", "PRIME-ClearCycles": "2", "520-Name": "DA Shutter", "405 Shutter-Name": "AnalogIO", "PIEZO-StepSize": "0.0050", "PRIME-ClearMode": "Pre-Exposure", "470 AO-MinVolts": "0.0000", "405 AO-SupportsTriggering": "1", "CameraChannelIndex": 0, "XYStage-StartSpeed": "500.0000", "COM9-StopBits": "2", "405 AO-Description": "NI DAC", "COM4-DelayBetweenCharsMs": "0.0000", "445 AO-Block voltage": "false", "520 AO-TriggerInputLine": "/Dev1/PFI0", "555 AO-Description": "NI DAC", "520 AO-TriggerSampleRate": "100000", "COM4-Verbose": "1", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM4-Name": "COM4", "405 Shutter-Volts": "5.0000", "Vincent-D3-Port": "COM1", "PRIME-PP 4 MIN ADU AFFECTED": "200", "470 Shutter-MinVolts": "0.0000", "PRIME-PP 3 ENABLED": "Yes", "470 AO-Description": "NI DAC", "PRIME-Description": "PVCAM API device adapter", "520 Shutter-MaxVolts": "5.0000", "520 Shutter-Volts": "0.0000", "528 AO-Sequenceable": "1", "PRIME-Binning": "1x1", "555 Shutter-MaxVolts": "5.0000", "528 Shutter-TriggerSequenceLength": "1000", "405 Shutter-Block voltage": "false", "XLIGHT Spin motor-State": "1", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "Vincent-D3-Time to open (ms)": "35.0000", "Vincent-D3-Time to close (ms)": "35.0000", "445 AO-TriggerSampleRate": "100000", "640 Shutter-IOChannel": "Dev1/ao14", "555 AO-Sequenceable": "1", "520 AO-Name": "AnalogIO", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "470 AO-Block voltage": "false", "COM1-Verbose": "1", "COM9-AnswerTimeout": "10000.0000", "528 Shutter-Name": "AnalogIO", "PRIME-Trigger Timeout (secs)": "10", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "NextFrame": 0, "470 AO-TriggerSequenceLength": "1000", "PRIME-TransposeXY": "0", "445 AO-TriggerSequenceLength": "1000", "COM1-Name": "COM1", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "COM9-DataBits": "8", "COM1-AnswerTimeout": "500.0000", "Camera": "", "TE2000-Version": "V2.50-V2.50-V2.10", "528 Shutter-Volts": "0.0000", "XLIGHT Hub-Name": "XLIGHT Hub", "PIEZO-Description": "Ludl stage driver adapter", "PositionName": "Pos3", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSampleRate": "100000", "PRIME-FanSpeedSetpoint": "High", "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "405 AO-TriggerSampleRate": "100000", "470 Shutter-Block voltage": "false", "470-State": "0", "COM3-Parity": "None", "405 AO-Volts": "4.5000", "PRIME-TransposeMirrorY": "0", "PRIME-ChipName": "GS144BSI", "470 AO-Volts": "0.0000", "PRIME-TransposeMirrorX": "0", "445 Shutter-SupportsTriggering": "0", "470 Shutter-SupportsTriggering": "0", "XLIGHT Hub-Description": "XLIGHT hub", "PRIME-PreampOffLimit": "10000", "470 AO-Sequenceable": "1", "528 Shutter-MaxVolts": "5.0000", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "Core-XYStage": "XYStage", "470 AO-TriggerSampleRate": "100000", "PRIME-Timing-PreTriggerDelayNs": "24672000.0000", "528-DA Device": "528 Shutter", "Focus-Description": "Nikon TE2000 focus adapter", "FilterBlock-State": "5", "520 AO-Demo": "No", "COM3-DelayBetweenCharsMs": "0.0000", "470 Shutter-Volts": "0.0000", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "PRIME-PP 2 THRESHOLD": "125", "PRIME-PP 4 THRESHOLD": "75", "XYStage-Acceleration": "4.0000", "470 Shutter-Demo": "No", "PRIME-Name": "PMPCIECam00", "640-DA Device": "640 Shutter", "405 AO-Block voltage": "false", "ElapsedTime-ms": 167722, "PRIME-Offset": "100", "445 AO-IOChannel": "Dev1/ao1", "640 AO-MaxVolts": "5.0000", "PositionIndex": 3, "PRIME-PP 1 ENABLED": "Yes", "COM1-BaudRate": "9600", "640-State": "0", "COM3-Verbose": "1", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "LudlController-TransmissionDelay": "0", "PRIME-FullWellCapacity": "62000", "PRIME-SMARTStreamingEnabled": "No", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "640 Shutter-Description": "NI DAC", "528-Description": "DA device that is used as a shutter", "COM4-Description": "Serial port driver (boost:asio)", "405 Shutter-Description": "NI DAC", "TE2000-Name": "TE2000", "528 Shutter-Description": "NI DAC", "COM9-Description": "Serial port driver (boost:asio)", "LudlController-Name": "LudlController", "445 Shutter-TriggerSequenceLength": "1000", "PRIME-SerialNumber": "A18B203008", "445 AO-Volts": "0.0000", "PRIME-X-dimension": "1200", "PRIME-ShutterCloseDelay": "0", "XLIGHT Disk slider-State": "2", "Position": "Default", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 AO-IOChannel": "Dev1/ao5", "520 AO-IOChannel": "Dev1/ao4", "528 AO-TriggerSequenceLength": "1000", "405 Shutter-MaxVolts": "5.0000", "470-DA Device": "470 Shutter", "PRIME-FTCapable": "No", "Core-ChannelGroup": "Channels", "555 AO-SupportsTriggering": "0", "PRIME-Y-dimension": "1200", "Frame": 0, "555 Shutter-Sequenceable": "1", "PRIME-Timing-ExposureTimeNs": "9992000.0000", "Core-ImageProcessor": "", "528-State": "0", "520 AO-Block voltage": "false", "XLIGHT Excitation Wheel-Label": "QUAD", "640 Shutter-Demo": "No", "COM3-StopBits": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "520 Shutter-IOChannel": "Dev1/ao11", "Width": 1200, "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-SupportsTriggering": "0", "640 AO-TriggerSampleRate": "100000", "520-Description": "DA device that is used as a shutter", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "405 Shutter-Sequenceable": "1", "405 Shutter-SupportsTriggering": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-TriggerSampleRate": "100000", "640 Shutter-MaxVolts": "5.0000", "555 AO-MaxVolts": "5.0000", "520 AO-TriggerSequenceLength": "1000", "COM3-BaudRate": "9600", "COM9-BaudRate": "9600", "Vincent-D3-Channel #": "Ch. #1", "PRIME-PMode": "Normal", "OpticalPath-Label": "Left(100)", "BitDepth": 16, "555 AO-TriggerSequenceLength": "1000", "445 Shutter-Description": "NI DAC", "405 AO-Sequenceable": "1", "PRIME-AcquisitionMethod": "Callbacks", "555 AO-Name": "AnalogIO", "PRIME-ShutterMode": "Pre-Exposure", "COM3-Handshaking": "Off", "445 AO-SupportsTriggering": "0", "ROI": "0-0-1200-1200", "COM9-Verbose": "1", "PRIME-Color": "OFF", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "PRIME-ShutterOpenDelay": "0", "COM1-Parity": "None", "Slice": 7, "SlicePosition": 20, "COM9-DelayBetweenCharsMs": "0.0000", "555-Name": "DA Shutter", "PIEZO-LudlSingleAxisName": "B", "XLIGHT Emission Wheel-Label": "QUAD", "640 Shutter-Name": "AnalogIO", "640 AO-Sequenceable": "1", "520 Shutter-Demo": "No", "528 Shutter-Demo": "No", "470-Name": "DA Shutter", "520-DA Device": "520 Shutter", "XYStage-Description": "Ludl XY stage driver adapter", "445 Shutter-MinVolts": "0.0000", "528 AO-TriggerSampleRate": "100000", "405 AO-MinVolts": "0.0000", "405 Shutter-TriggerSequenceLength": "1000", "Height": 1200, "555 AO-Block voltage": "false", "640 Shutter-Volts": "0.0000", "PixelType": "GRAY16", "445 Shutter-Block voltage": "false", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "520 Shutter-TriggerSequenceLength": "1000", "COM9-Name": "COM9", "Core-SLM": "", "555 AO-Volts": "0.0000", "555 Shutter-Block voltage": "false", "XYStage-Speed": "1800.0000", "520 AO-MaxVolts": "5.0000", "528 Shutter-SupportsTriggering": "0", "COM1-Description": "Serial port driver (boost:asio)", "PRIME-MetadataEnabled": "No", "520 Shutter-MinVolts": "0.0000", "470 Shutter-Sequenceable": "1", "PRIME-PreampDelay": "0", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-MinVolts": "0.0000", "ChannelIndex": 5, "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Core-AutoShutter": "1", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "Exposure-ms": 300, "PRIME-PP 2 ENABLED": "Yes", "640 Shutter-TriggerSampleRate": "100000", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "470 Shutter-IOChannel": "Dev1/ao10", "405 AO-Demo": "No", "PixelSizeUm": 1.1, "470 AO-Demo": "No", "470 Shutter-MaxVolts": "5.0000", "Vincent-D3-Name": "Vincent-D3", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FileName": "img_000000000_P-DAPI-high_007.tif", "LudlController-Reset": "Operate", "PRIME-Exposure": "300.00", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "445-Name": "DA Shutter", "555 Shutter-Name": "AnalogIO", "520 Shutter-TriggerSampleRate": "100000", "445 Shutter-IOChannel": "Dev1/ao9", "PRIME-TransposeCorrection": "0", "XYStage-StepSize": "0.1000", "445-Description": "DA device that is used as a shutter", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 AO-IOChannel": "Dev1/ao7", "520 Shutter-Name": "AnalogIO", "COM3-DataBits": "8", "640 AO-Volts": "0.0000", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "555 Shutter-IOChannel": "Dev1/ao13", "WaitInterval": null, "520 Shutter-Sequenceable": "1", "YPositionUm": 20690.300000000003, "445-State": "0", "445-DA Device": "445 Shutter", "PRIME-CircularBufferAutoSize": "ON", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "555 AO-TriggerSampleRate": "100000", "445 Shutter-Volts": "0.0000", "XLIGHT Hub-Port": "COM3", "Core-AutoFocus": "", "COM4-Handshaking": "Off", "528 Shutter-MinVolts": "0.0000", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "445 Shutter-Name": "AnalogIO", "528 Shutter-Sequenceable": "1", "XYStage-TransposeMirrorX": "0", "XYStage-TransposeMirrorY": "0", "PRIME-ReadoutRate": "100MHz 16bit", "XYStage-Name": "Stage", "640 AO-SupportsTriggering": "0", "640-Name": "DA Shutter", "640 Shutter-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "640 AO-Block voltage": "false", "445 Shutter-Sequenceable": "1", "470 AO-MaxVolts": "5.0000", "COM4-BaudRate": "9600", "470-Description": "DA device that is used as a shutter", "520 Shutter-Description": "NI DAC", "PRIME-CCDTemperature": "-14.9600", "Time": "2019-12-17 21:59:15 -0500", "640 AO-TriggerSequenceLength": "1000", "470 AO-Name": "AnalogIO", "XLIGHT Disk slider-Label": "Disk Pos 40um", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "Binning": "1x1", "TE2000-ModelType": "1", "COM3-AnswerTimeout": "500.0000", "555 Shutter-SupportsTriggering": "0", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "TE2000-Port": "COM4", "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Description": "NI DAC", "COM4-AnswerTimeout": "500.0000", "COM1-DelayBetweenCharsMs": "0.0000", "FilterBlock-Label": "Confocal", "445 AO-Name": "AnalogIO", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-Timing-ReadoutTimeNs": "24672000.0000", "528 AO-Demo": "No", "520 AO-Sequenceable": "1", "555-Description": "DA device that is used as a shutter", "405-DA Device": "405 Shutter", "405 AO-TriggerSequenceLength": "1000", "640 AO-MinVolts": "0.0000", "640 Shutter-Block voltage": "false", "FrameIndex": 0, "PRIME-Port": "CMOS", "555 AO-MinVolts": "0.0000", "COM3-Description": "Serial port driver (boost:asio)", "528 AO-Description": "NI DAC", "555 AO-IOChannel": "Dev1/ao6", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "555 AO-TriggerInputLine": "/Dev1/PFI0", "PRIME-ActualInterval-ms": "374.3700", "LudlController-Port": "COM9", "445 Shutter-Demo": "No", "PRIME-Trigger-Expose Out-Mux": "1", "SliceIndex": 7, "PRIME-CCDTemperatureSetPoint": "-15.0000", "405 Shutter-MinVolts": "0.0000", "PRIME-ExposeOutMode": "First Row", "AxisPositions": null, "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Volts": "0.0000", "528-Name": "DA Shutter", "555-State": "0", "XLIGHT Dichroic-Label": "QUAD", "445 AO-Sequenceable": "1", "405-Name": "DA Shutter", "405 AO-IOChannel": "Dev1/ao0", "Core-Camera": "PRIME", "Focus-Name": "Focus", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "405 Shutter-IOChannel": "Dev1/ao8", "640 AO-Demo": "No", "640 Shutter-SupportsTriggering": "0", "445 AO-Demo": "No", "555-DA Device": "555 Shutter", "470 Shutter-Description": "NI DAC", "528 AO-Volts": "0.0000", "COM1-DataBits": "8", "Vincent-D3-Address": "x", "520 AO-SupportsTriggering": "0", "405-Description": "DA device that is used as a shutter", "640 Shutter-MinVolts": "0.0000", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "405 AO-Name": "AnalogIO", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "640 AO-TriggerInputLine": "/Dev1/PFI0", "405 AO-TriggerInputLine": "/Dev1/PFI0", "640-Description": "DA device that is used as a shutter", "445 Shutter-TriggerSampleRate": "100000", "470 Shutter-TriggerSequenceLength": "1000", "COM1-StopBits": "1", "520 Shutter-SupportsTriggering": "0", "405-State": "1", "Core-Initialize": "1", "XLIGHT Emission Wheel-State": "7", "520 AO-Description": "NI DAC", "528 AO-TriggerInputLine": "/Dev1/PFI0", "445 Shutter-MaxVolts": "5.0000", "COM9-Handshaking": "Off", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "XLIGHT Spin motor-Label": "On (spinning)", "PRIME-PP 5 ENABLED": "No", "UUID": "4270caaf-ec61-4543-8e84-2f8a488a3b4c", "PRIME-TriggerMode": "Internal Trigger", "COM1-Handshaking": "Off", "PRIME-Timing-PostTriggerDelayNs": "49344000.0000", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "Focus-Position": "16479.8000", "528 Shutter-Block voltage": "false", "Channel": "P-DAPI-high", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "555 Shutter-Demo": "No", "405 Shutter-Demo": "No", "528 AO-Name": "AnalogIO", "520-State": "0", "Core-Galvo": "", "405 Shutter-TriggerSampleRate": "100000", "Focus-FocusStepSize_(nm)": "100", "PRIME-PixelType": "16bit", "COM4-DataBits": "8", "470 AO-IOChannel": "Dev1/ao2", "520 AO-MinVolts": "0.0000", "COM9-Parity": "None", "PRIME-CircularBufferEnabled": "ON", "PRIME-PP 6 Reset": "No", "640 AO-Description": "NI DAC", "Source": "PRIME", "445 AO-MinVolts": "0.0000", "PIEZO-Name": "Stage", "445 AO-Description": "NI DAC", "PRIME-CircularBufferFrameCount": "46", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "520 AO-Volts": "0.0000", "528 Shutter-TriggerSampleRate": "100000", "XPositionUm": 62321, "OpticalPath-State": "4", "PRIME-PP 3 THRESHOLD": "80", "PRIME-PVCAM Version": "3.7.1", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Gain": "1-HDR", "640 Shutter-TriggerSequenceLength": "1000", "528 AO-SupportsTriggering": "0", "445 AO-TriggerInputLine": "/Dev1/PFI0", "OpticalPath-Name": "OpticalPath", "PRIME-PP 4 ENABLED": "Yes", "PRIME-PVCAM Adapter Version": "1.3.29", "COM3-Name": "COM3", "405 AO-MaxVolts": "5.0000", "520 Shutter-Block voltage": "false", "PRIME-Timing-ClearingTimeNs": "49344000.0000", "Core-Focus": "PIEZO", "528 AO-Block voltage": "false", "TE2000-Description": "Nikon TE2000 hub", "528 AO-MinVolts": "0.0000", "COM4-Parity": "None", "Summary": { "Slices": 11, "ChContrastMin": [ 0, 0, 0, 0, 0, 0 ], "PixelAspect": 1, "SlicesFirst": true, "Interval_ms": 0, "Time": "2019-12-17 21:58:39 -0500", "Prefix": "seq_3", "InitialPositionList": [ { "Label": "Pos0", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.435], "Focus": [16480.2], "XYStage": [ 69822.40000000001, 20100.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos1", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.425], "Focus": [16480.3], "XYStage": [ 69004, 20101 ] }, "GridColumnIndex": 0 }, { "Label": "Pos2", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.44], "Focus": [16480.1], "XYStage": [ 63119.5, 20690.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos3", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.405], "Focus": [16480.2], "XYStage": [ 62321, 20690.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos4", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.415], "Focus": [16480], "XYStage": [ 56604.100000000006, 20688.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos5", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.42000000000002], "Focus": [16480.1], "XYStage": [ 56034.200000000004, 20688.800000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos6", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.455], "Focus": [16480], "XYStage": [ 50889.3, 20496.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos7", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.43], "Focus": [16480.2], "XYStage": [ 50101.600000000006, 20496.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos8", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.67000000000002], "Focus": [16479.9], "XYStage": [ 44818.600000000006, 20496.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos9", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.67000000000002], "Focus": [16480], "XYStage": [ 43897.9, 20436.800000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos10", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.675], "Focus": [16479.9], "XYStage": [ 39670.600000000006, 20664.9 ] }, "GridColumnIndex": 0 }, { "Label": "Pos11", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.69], "Focus": [16479.9], "XYStage": [ 38734, 20664.600000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos12", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.695], "Focus": [16480.1], "XYStage": [ 34037.200000000004, 20664.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos13", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.685], "Focus": [16480.1], "XYStage": [ 33152, 20664.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos14", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.69], "Focus": [16480.1], "XYStage": [ 28122.800000000003, 20664.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos15", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.675], "Focus": [16480], "XYStage": [ 27422.9, 20664.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos16", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.64000000000001], "Focus": [16480], "XYStage": [ 23005.2, 20664.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos17", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.69], "Focus": [16479.9], "XYStage": [ 21820.600000000002, 20409.600000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos18", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.41], "Focus": [16480.1], "XYStage": [ 73697.6, -13222.800000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos19", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.455], "Focus": [16480.1], "XYStage": [ 72765.8, -13223 ] }, "GridColumnIndex": 0 }, { "Label": "Pos20", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.4], "Focus": [16480.2], "XYStage": [ 67445.5, -12303.800000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos21", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.415], "Focus": [16480.2], "XYStage": [ 66613.3, -12303.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos22", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.415], "Focus": [16480.1], "XYStage": [ 61819.4, -11936.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos23", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.405], "Focus": [16480.2], "XYStage": [ 61325.700000000004, -11937.1 ] }, "GridColumnIndex": 0 }, { "Label": "Pos24", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.46], "Focus": [16480.2], "XYStage": [ 55377, -10974.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos25", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.445], "Focus": [16480.1], "XYStage": [ 54827, -11214.800000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos26", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.435], "Focus": [16480.2], "XYStage": [ 49937.100000000006, -10919.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos27", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.425], "Focus": [16480.1], "XYStage": [ 48764, -10919.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos28", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.42000000000002], "Focus": [16480.2], "XYStage": [ 43978.4, -10920.400000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos29", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.435], "Focus": [16480.2], "XYStage": [ 43106.3, -10920.400000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos30", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.405], "Focus": [16480.3], "XYStage": [ 38212.4, -10920.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos31", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.445], "Focus": [16480.2], "XYStage": [ 37254.9, -10920.400000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos32", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.44], "Focus": [16480.3], "XYStage": [ 32472.800000000003, -11294.400000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos33", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [166.95000000000002], "Focus": [16480.2], "XYStage": [ 31420.7, -11294.400000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos34", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [166.95000000000002], "Focus": [16480.2], "XYStage": [ 26881.5, -11640 ] }, "GridColumnIndex": 0 }, { "Label": "Pos35", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [166.98], "Focus": [16480.2], "XYStage": [ 25876.100000000002, -11641.1 ] }, "GridColumnIndex": 0 } ], "z-step_um": 10, "GridRow": 0, "BitDepth": 16, "Depth": 2, "Channels": 6, "Source": "Micro-Manager", "ChColors": [ -16711681, -256, -65281, -1, -1, -16776961 ], "MetadataVersion": 10, "KeepShutterOpenChannels": true, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536, 65536 ], "CustomIntervals_ms": [], "Height": 1200, "MicroManagerVersion": "1.4.23 20180219", "GridColumn": 0, "UUID": "8c8f9d20-b128-4577-9f91-89e70f22d319", "PixelType": "GRAY16", "Width": 1200, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\Yan\\20191217-YC113-S5S6", "Frames": 1, "Comment": "", "UserName": "Nikon", "PixelSize_um": 1.1, "ROI": [ 0, 0, 1200, 1200 ], "Date": "2019-12-17", "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC", "P-DAPI-high" ], "KeepShutterOpenSlices": true, "IJType": 1, "ComputerName": "ZadorConfocal2", "Positions": 36, "PositionIndex": 3, "CameraTimeout": 20000, "TimeFirst": false }, "640 AO-Name": "AnalogIO", "Vincent-D3-Command": "Close" }, "FrameKey-0-5-8": { "COM4-StopBits": "1", "528 AO-MaxVolts": "5.0000", "XLIGHT Excitation Wheel-State": "2", "470 Shutter-Name": "AnalogIO", "Core-Shutter": "405", "555 AO-Demo": "No", "XLIGHT Dichroic-State": "2", "XYStage-StepSize-Y": "0.1000", "ZPositionUm": 207.405, "XYStage-StepSize-X": "0.1000", "PRIME-FirmwareVersion": "20.28", "PRIME-ClearCycles": "2", "520-Name": "DA Shutter", "405 Shutter-Name": "AnalogIO", "PIEZO-StepSize": "0.0050", "PRIME-ClearMode": "Pre-Exposure", "470 AO-MinVolts": "0.0000", "405 AO-SupportsTriggering": "1", "CameraChannelIndex": 0, "XYStage-StartSpeed": "500.0000", "COM9-StopBits": "2", "405 AO-Description": "NI DAC", "COM4-DelayBetweenCharsMs": "0.0000", "445 AO-Block voltage": "false", "520 AO-TriggerInputLine": "/Dev1/PFI0", "555 AO-Description": "NI DAC", "520 AO-TriggerSampleRate": "100000", "COM4-Verbose": "1", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM4-Name": "COM4", "405 Shutter-Volts": "5.0000", "Vincent-D3-Port": "COM1", "PRIME-PP 4 MIN ADU AFFECTED": "200", "470 Shutter-MinVolts": "0.0000", "PRIME-PP 3 ENABLED": "Yes", "470 AO-Description": "NI DAC", "PRIME-Description": "PVCAM API device adapter", "520 Shutter-MaxVolts": "5.0000", "520 Shutter-Volts": "0.0000", "528 AO-Sequenceable": "1", "PRIME-Binning": "1x1", "555 Shutter-MaxVolts": "5.0000", "528 Shutter-TriggerSequenceLength": "1000", "405 Shutter-Block voltage": "false", "XLIGHT Spin motor-State": "1", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "Vincent-D3-Time to open (ms)": "35.0000", "Vincent-D3-Time to close (ms)": "35.0000", "445 AO-TriggerSampleRate": "100000", "640 Shutter-IOChannel": "Dev1/ao14", "555 AO-Sequenceable": "1", "520 AO-Name": "AnalogIO", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "470 AO-Block voltage": "false", "COM1-Verbose": "1", "COM9-AnswerTimeout": "10000.0000", "528 Shutter-Name": "AnalogIO", "PRIME-Trigger Timeout (secs)": "10", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "NextFrame": 0, "470 AO-TriggerSequenceLength": "1000", "PRIME-TransposeXY": "0", "445 AO-TriggerSequenceLength": "1000", "COM1-Name": "COM1", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "COM9-DataBits": "8", "COM1-AnswerTimeout": "500.0000", "Camera": "", "TE2000-Version": "V2.50-V2.50-V2.10", "528 Shutter-Volts": "0.0000", "XLIGHT Hub-Name": "XLIGHT Hub", "PIEZO-Description": "Ludl stage driver adapter", "PositionName": "Pos3", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSampleRate": "100000", "PRIME-FanSpeedSetpoint": "High", "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "405 AO-TriggerSampleRate": "100000", "470 Shutter-Block voltage": "false", "470-State": "0", "COM3-Parity": "None", "405 AO-Volts": "4.5000", "PRIME-TransposeMirrorY": "0", "PRIME-ChipName": "GS144BSI", "470 AO-Volts": "0.0000", "PRIME-TransposeMirrorX": "0", "445 Shutter-SupportsTriggering": "0", "470 Shutter-SupportsTriggering": "0", "XLIGHT Hub-Description": "XLIGHT hub", "PRIME-PreampOffLimit": "10000", "470 AO-Sequenceable": "1", "528 Shutter-MaxVolts": "5.0000", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "Core-XYStage": "XYStage", "470 AO-TriggerSampleRate": "100000", "PRIME-Timing-PreTriggerDelayNs": "24672000.0000", "528-DA Device": "528 Shutter", "Focus-Description": "Nikon TE2000 focus adapter", "FilterBlock-State": "5", "520 AO-Demo": "No", "COM3-DelayBetweenCharsMs": "0.0000", "470 Shutter-Volts": "0.0000", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "PRIME-PP 2 THRESHOLD": "125", "PRIME-PP 4 THRESHOLD": "75", "XYStage-Acceleration": "4.0000", "470 Shutter-Demo": "No", "PRIME-Name": "PMPCIECam00", "640-DA Device": "640 Shutter", "405 AO-Block voltage": "false", "ElapsedTime-ms": 168263, "PRIME-Offset": "100", "445 AO-IOChannel": "Dev1/ao1", "640 AO-MaxVolts": "5.0000", "PositionIndex": 3, "PRIME-PP 1 ENABLED": "Yes", "COM1-BaudRate": "9600", "640-State": "0", "COM3-Verbose": "1", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "LudlController-TransmissionDelay": "0", "PRIME-FullWellCapacity": "62000", "PRIME-SMARTStreamingEnabled": "No", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "640 Shutter-Description": "NI DAC", "528-Description": "DA device that is used as a shutter", "COM4-Description": "Serial port driver (boost:asio)", "405 Shutter-Description": "NI DAC", "TE2000-Name": "TE2000", "528 Shutter-Description": "NI DAC", "COM9-Description": "Serial port driver (boost:asio)", "LudlController-Name": "LudlController", "445 Shutter-TriggerSequenceLength": "1000", "PRIME-SerialNumber": "A18B203008", "445 AO-Volts": "0.0000", "PRIME-X-dimension": "1200", "PRIME-ShutterCloseDelay": "0", "XLIGHT Disk slider-State": "2", "Position": "Default", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 AO-IOChannel": "Dev1/ao5", "520 AO-IOChannel": "Dev1/ao4", "528 AO-TriggerSequenceLength": "1000", "405 Shutter-MaxVolts": "5.0000", "470-DA Device": "470 Shutter", "PRIME-FTCapable": "No", "Core-ChannelGroup": "Channels", "555 AO-SupportsTriggering": "0", "PRIME-Y-dimension": "1200", "Frame": 0, "555 Shutter-Sequenceable": "1", "PRIME-Timing-ExposureTimeNs": "9992000.0000", "Core-ImageProcessor": "", "528-State": "0", "520 AO-Block voltage": "false", "XLIGHT Excitation Wheel-Label": "QUAD", "640 Shutter-Demo": "No", "COM3-StopBits": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "520 Shutter-IOChannel": "Dev1/ao11", "Width": 1200, "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-SupportsTriggering": "0", "640 AO-TriggerSampleRate": "100000", "520-Description": "DA device that is used as a shutter", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "405 Shutter-Sequenceable": "1", "405 Shutter-SupportsTriggering": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-TriggerSampleRate": "100000", "640 Shutter-MaxVolts": "5.0000", "555 AO-MaxVolts": "5.0000", "520 AO-TriggerSequenceLength": "1000", "COM3-BaudRate": "9600", "COM9-BaudRate": "9600", "Vincent-D3-Channel #": "Ch. #1", "PRIME-PMode": "Normal", "OpticalPath-Label": "Left(100)", "BitDepth": 16, "555 AO-TriggerSequenceLength": "1000", "445 Shutter-Description": "NI DAC", "405 AO-Sequenceable": "1", "PRIME-AcquisitionMethod": "Callbacks", "555 AO-Name": "AnalogIO", "PRIME-ShutterMode": "Pre-Exposure", "COM3-Handshaking": "Off", "445 AO-SupportsTriggering": "0", "ROI": "0-0-1200-1200", "COM9-Verbose": "1", "PRIME-Color": "OFF", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "PRIME-ShutterOpenDelay": "0", "COM1-Parity": "None", "Slice": 8, "SlicePosition": 30, "COM9-DelayBetweenCharsMs": "0.0000", "555-Name": "DA Shutter", "PIEZO-LudlSingleAxisName": "B", "XLIGHT Emission Wheel-Label": "QUAD", "640 Shutter-Name": "AnalogIO", "640 AO-Sequenceable": "1", "520 Shutter-Demo": "No", "528 Shutter-Demo": "No", "470-Name": "DA Shutter", "520-DA Device": "520 Shutter", "XYStage-Description": "Ludl XY stage driver adapter", "445 Shutter-MinVolts": "0.0000", "528 AO-TriggerSampleRate": "100000", "405 AO-MinVolts": "0.0000", "405 Shutter-TriggerSequenceLength": "1000", "Height": 1200, "555 AO-Block voltage": "false", "640 Shutter-Volts": "0.0000", "PixelType": "GRAY16", "445 Shutter-Block voltage": "false", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "520 Shutter-TriggerSequenceLength": "1000", "COM9-Name": "COM9", "Core-SLM": "", "555 AO-Volts": "0.0000", "555 Shutter-Block voltage": "false", "XYStage-Speed": "1800.0000", "520 AO-MaxVolts": "5.0000", "528 Shutter-SupportsTriggering": "0", "COM1-Description": "Serial port driver (boost:asio)", "PRIME-MetadataEnabled": "No", "520 Shutter-MinVolts": "0.0000", "470 Shutter-Sequenceable": "1", "PRIME-PreampDelay": "0", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-MinVolts": "0.0000", "ChannelIndex": 5, "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Core-AutoShutter": "1", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "Exposure-ms": 300, "PRIME-PP 2 ENABLED": "Yes", "640 Shutter-TriggerSampleRate": "100000", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "470 Shutter-IOChannel": "Dev1/ao10", "405 AO-Demo": "No", "PixelSizeUm": 1.1, "470 AO-Demo": "No", "470 Shutter-MaxVolts": "5.0000", "Vincent-D3-Name": "Vincent-D3", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FileName": "img_000000000_P-DAPI-high_008.tif", "LudlController-Reset": "Operate", "PRIME-Exposure": "300.00", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "445-Name": "DA Shutter", "555 Shutter-Name": "AnalogIO", "520 Shutter-TriggerSampleRate": "100000", "445 Shutter-IOChannel": "Dev1/ao9", "PRIME-TransposeCorrection": "0", "XYStage-StepSize": "0.1000", "445-Description": "DA device that is used as a shutter", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 AO-IOChannel": "Dev1/ao7", "520 Shutter-Name": "AnalogIO", "COM3-DataBits": "8", "640 AO-Volts": "0.0000", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "555 Shutter-IOChannel": "Dev1/ao13", "WaitInterval": null, "520 Shutter-Sequenceable": "1", "YPositionUm": 20690.300000000003, "445-State": "0", "445-DA Device": "445 Shutter", "PRIME-CircularBufferAutoSize": "ON", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "555 AO-TriggerSampleRate": "100000", "445 Shutter-Volts": "0.0000", "XLIGHT Hub-Port": "COM3", "Core-AutoFocus": "", "COM4-Handshaking": "Off", "528 Shutter-MinVolts": "0.0000", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "445 Shutter-Name": "AnalogIO", "528 Shutter-Sequenceable": "1", "XYStage-TransposeMirrorX": "0", "XYStage-TransposeMirrorY": "0", "PRIME-ReadoutRate": "100MHz 16bit", "XYStage-Name": "Stage", "640 AO-SupportsTriggering": "0", "640-Name": "DA Shutter", "640 Shutter-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "640 AO-Block voltage": "false", "445 Shutter-Sequenceable": "1", "470 AO-MaxVolts": "5.0000", "COM4-BaudRate": "9600", "470-Description": "DA device that is used as a shutter", "520 Shutter-Description": "NI DAC", "PRIME-CCDTemperature": "-14.9600", "Time": "2019-12-17 21:59:15 -0500", "640 AO-TriggerSequenceLength": "1000", "470 AO-Name": "AnalogIO", "XLIGHT Disk slider-Label": "Disk Pos 40um", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "Binning": "1x1", "TE2000-ModelType": "1", "COM3-AnswerTimeout": "500.0000", "555 Shutter-SupportsTriggering": "0", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "TE2000-Port": "COM4", "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Description": "NI DAC", "COM4-AnswerTimeout": "500.0000", "COM1-DelayBetweenCharsMs": "0.0000", "FilterBlock-Label": "Confocal", "445 AO-Name": "AnalogIO", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-Timing-ReadoutTimeNs": "24672000.0000", "528 AO-Demo": "No", "520 AO-Sequenceable": "1", "555-Description": "DA device that is used as a shutter", "405-DA Device": "405 Shutter", "405 AO-TriggerSequenceLength": "1000", "640 AO-MinVolts": "0.0000", "640 Shutter-Block voltage": "false", "FrameIndex": 0, "PRIME-Port": "CMOS", "555 AO-MinVolts": "0.0000", "COM3-Description": "Serial port driver (boost:asio)", "528 AO-Description": "NI DAC", "555 AO-IOChannel": "Dev1/ao6", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "555 AO-TriggerInputLine": "/Dev1/PFI0", "PRIME-ActualInterval-ms": "374.3700", "LudlController-Port": "COM9", "445 Shutter-Demo": "No", "PRIME-Trigger-Expose Out-Mux": "1", "SliceIndex": 8, "PRIME-CCDTemperatureSetPoint": "-15.0000", "405 Shutter-MinVolts": "0.0000", "PRIME-ExposeOutMode": "First Row", "AxisPositions": null, "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Volts": "0.0000", "528-Name": "DA Shutter", "555-State": "0", "XLIGHT Dichroic-Label": "QUAD", "445 AO-Sequenceable": "1", "405-Name": "DA Shutter", "405 AO-IOChannel": "Dev1/ao0", "Core-Camera": "PRIME", "Focus-Name": "Focus", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "405 Shutter-IOChannel": "Dev1/ao8", "640 AO-Demo": "No", "640 Shutter-SupportsTriggering": "0", "445 AO-Demo": "No", "555-DA Device": "555 Shutter", "470 Shutter-Description": "NI DAC", "528 AO-Volts": "0.0000", "COM1-DataBits": "8", "Vincent-D3-Address": "x", "520 AO-SupportsTriggering": "0", "405-Description": "DA device that is used as a shutter", "640 Shutter-MinVolts": "0.0000", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "405 AO-Name": "AnalogIO", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "640 AO-TriggerInputLine": "/Dev1/PFI0", "405 AO-TriggerInputLine": "/Dev1/PFI0", "640-Description": "DA device that is used as a shutter", "445 Shutter-TriggerSampleRate": "100000", "470 Shutter-TriggerSequenceLength": "1000", "COM1-StopBits": "1", "520 Shutter-SupportsTriggering": "0", "405-State": "1", "Core-Initialize": "1", "XLIGHT Emission Wheel-State": "7", "520 AO-Description": "NI DAC", "528 AO-TriggerInputLine": "/Dev1/PFI0", "445 Shutter-MaxVolts": "5.0000", "COM9-Handshaking": "Off", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "XLIGHT Spin motor-Label": "On (spinning)", "PRIME-PP 5 ENABLED": "No", "UUID": "88c4a03a-28e8-4510-b049-35e8b9e81191", "PRIME-TriggerMode": "Internal Trigger", "COM1-Handshaking": "Off", "PRIME-Timing-PostTriggerDelayNs": "49344000.0000", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "Focus-Position": "16479.8000", "528 Shutter-Block voltage": "false", "Channel": "P-DAPI-high", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "555 Shutter-Demo": "No", "405 Shutter-Demo": "No", "528 AO-Name": "AnalogIO", "520-State": "0", "Core-Galvo": "", "405 Shutter-TriggerSampleRate": "100000", "Focus-FocusStepSize_(nm)": "100", "PRIME-PixelType": "16bit", "COM4-DataBits": "8", "470 AO-IOChannel": "Dev1/ao2", "520 AO-MinVolts": "0.0000", "COM9-Parity": "None", "PRIME-CircularBufferEnabled": "ON", "PRIME-PP 6 Reset": "No", "640 AO-Description": "NI DAC", "Source": "PRIME", "445 AO-MinVolts": "0.0000", "PIEZO-Name": "Stage", "445 AO-Description": "NI DAC", "PRIME-CircularBufferFrameCount": "46", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "520 AO-Volts": "0.0000", "528 Shutter-TriggerSampleRate": "100000", "XPositionUm": 62321, "OpticalPath-State": "4", "PRIME-PP 3 THRESHOLD": "80", "PRIME-PVCAM Version": "3.7.1", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Gain": "1-HDR", "640 Shutter-TriggerSequenceLength": "1000", "528 AO-SupportsTriggering": "0", "445 AO-TriggerInputLine": "/Dev1/PFI0", "OpticalPath-Name": "OpticalPath", "PRIME-PP 4 ENABLED": "Yes", "PRIME-PVCAM Adapter Version": "1.3.29", "COM3-Name": "COM3", "405 AO-MaxVolts": "5.0000", "520 Shutter-Block voltage": "false", "PRIME-Timing-ClearingTimeNs": "49344000.0000", "Core-Focus": "PIEZO", "528 AO-Block voltage": "false", "TE2000-Description": "Nikon TE2000 hub", "528 AO-MinVolts": "0.0000", "COM4-Parity": "None", "Summary": { "Slices": 11, "ChContrastMin": [ 0, 0, 0, 0, 0, 0 ], "PixelAspect": 1, "SlicesFirst": true, "Interval_ms": 0, "Time": "2019-12-17 21:58:39 -0500", "Prefix": "seq_3", "InitialPositionList": [ { "Label": "Pos0", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.435], "Focus": [16480.2], "XYStage": [ 69822.40000000001, 20100.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos1", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.425], "Focus": [16480.3], "XYStage": [ 69004, 20101 ] }, "GridColumnIndex": 0 }, { "Label": "Pos2", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.44], "Focus": [16480.1], "XYStage": [ 63119.5, 20690.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos3", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.405], "Focus": [16480.2], "XYStage": [ 62321, 20690.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos4", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.415], "Focus": [16480], "XYStage": [ 56604.100000000006, 20688.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos5", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.42000000000002], "Focus": [16480.1], "XYStage": [ 56034.200000000004, 20688.800000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos6", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.455], "Focus": [16480], "XYStage": [ 50889.3, 20496.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos7", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.43], "Focus": [16480.2], "XYStage": [ 50101.600000000006, 20496.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos8", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.67000000000002], "Focus": [16479.9], "XYStage": [ 44818.600000000006, 20496.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos9", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.67000000000002], "Focus": [16480], "XYStage": [ 43897.9, 20436.800000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos10", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.675], "Focus": [16479.9], "XYStage": [ 39670.600000000006, 20664.9 ] }, "GridColumnIndex": 0 }, { "Label": "Pos11", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.69], "Focus": [16479.9], "XYStage": [ 38734, 20664.600000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos12", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.695], "Focus": [16480.1], "XYStage": [ 34037.200000000004, 20664.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos13", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.685], "Focus": [16480.1], "XYStage": [ 33152, 20664.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos14", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.69], "Focus": [16480.1], "XYStage": [ 28122.800000000003, 20664.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos15", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.675], "Focus": [16480], "XYStage": [ 27422.9, 20664.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos16", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.64000000000001], "Focus": [16480], "XYStage": [ 23005.2, 20664.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos17", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.69], "Focus": [16479.9], "XYStage": [ 21820.600000000002, 20409.600000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos18", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.41], "Focus": [16480.1], "XYStage": [ 73697.6, -13222.800000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos19", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.455], "Focus": [16480.1], "XYStage": [ 72765.8, -13223 ] }, "GridColumnIndex": 0 }, { "Label": "Pos20", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.4], "Focus": [16480.2], "XYStage": [ 67445.5, -12303.800000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos21", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.415], "Focus": [16480.2], "XYStage": [ 66613.3, -12303.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos22", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.415], "Focus": [16480.1], "XYStage": [ 61819.4, -11936.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos23", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.405], "Focus": [16480.2], "XYStage": [ 61325.700000000004, -11937.1 ] }, "GridColumnIndex": 0 }, { "Label": "Pos24", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.46], "Focus": [16480.2], "XYStage": [ 55377, -10974.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos25", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.445], "Focus": [16480.1], "XYStage": [ 54827, -11214.800000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos26", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.435], "Focus": [16480.2], "XYStage": [ 49937.100000000006, -10919.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos27", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.425], "Focus": [16480.1], "XYStage": [ 48764, -10919.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos28", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.42000000000002], "Focus": [16480.2], "XYStage": [ 43978.4, -10920.400000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos29", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.435], "Focus": [16480.2], "XYStage": [ 43106.3, -10920.400000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos30", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.405], "Focus": [16480.3], "XYStage": [ 38212.4, -10920.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos31", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.445], "Focus": [16480.2], "XYStage": [ 37254.9, -10920.400000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos32", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.44], "Focus": [16480.3], "XYStage": [ 32472.800000000003, -11294.400000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos33", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [166.95000000000002], "Focus": [16480.2], "XYStage": [ 31420.7, -11294.400000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos34", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [166.95000000000002], "Focus": [16480.2], "XYStage": [ 26881.5, -11640 ] }, "GridColumnIndex": 0 }, { "Label": "Pos35", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [166.98], "Focus": [16480.2], "XYStage": [ 25876.100000000002, -11641.1 ] }, "GridColumnIndex": 0 } ], "z-step_um": 10, "GridRow": 0, "BitDepth": 16, "Depth": 2, "Channels": 6, "Source": "Micro-Manager", "ChColors": [ -16711681, -256, -65281, -1, -1, -16776961 ], "MetadataVersion": 10, "KeepShutterOpenChannels": true, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536, 65536 ], "CustomIntervals_ms": [], "Height": 1200, "MicroManagerVersion": "1.4.23 20180219", "GridColumn": 0, "UUID": "8c8f9d20-b128-4577-9f91-89e70f22d319", "PixelType": "GRAY16", "Width": 1200, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\Yan\\20191217-YC113-S5S6", "Frames": 1, "Comment": "", "UserName": "Nikon", "PixelSize_um": 1.1, "ROI": [ 0, 0, 1200, 1200 ], "Date": "2019-12-17", "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC", "P-DAPI-high" ], "KeepShutterOpenSlices": true, "IJType": 1, "ComputerName": "ZadorConfocal2", "Positions": 36, "PositionIndex": 3, "CameraTimeout": 20000, "TimeFirst": false }, "640 AO-Name": "AnalogIO", "Vincent-D3-Command": "Close" }, "FrameKey-0-5-9": { "COM4-StopBits": "1", "528 AO-MaxVolts": "5.0000", "XLIGHT Excitation Wheel-State": "2", "470 Shutter-Name": "AnalogIO", "Core-Shutter": "405", "555 AO-Demo": "No", "XLIGHT Dichroic-State": "2", "XYStage-StepSize-Y": "0.1000", "ZPositionUm": 217.405, "XYStage-StepSize-X": "0.1000", "PRIME-FirmwareVersion": "20.28", "PRIME-ClearCycles": "2", "520-Name": "DA Shutter", "405 Shutter-Name": "AnalogIO", "PIEZO-StepSize": "0.0050", "PRIME-ClearMode": "Pre-Exposure", "470 AO-MinVolts": "0.0000", "405 AO-SupportsTriggering": "1", "CameraChannelIndex": 0, "XYStage-StartSpeed": "500.0000", "COM9-StopBits": "2", "405 AO-Description": "NI DAC", "COM4-DelayBetweenCharsMs": "0.0000", "445 AO-Block voltage": "false", "520 AO-TriggerInputLine": "/Dev1/PFI0", "555 AO-Description": "NI DAC", "520 AO-TriggerSampleRate": "100000", "COM4-Verbose": "1", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM4-Name": "COM4", "405 Shutter-Volts": "5.0000", "Vincent-D3-Port": "COM1", "PRIME-PP 4 MIN ADU AFFECTED": "200", "470 Shutter-MinVolts": "0.0000", "PRIME-PP 3 ENABLED": "Yes", "470 AO-Description": "NI DAC", "PRIME-Description": "PVCAM API device adapter", "520 Shutter-MaxVolts": "5.0000", "520 Shutter-Volts": "0.0000", "528 AO-Sequenceable": "1", "PRIME-Binning": "1x1", "555 Shutter-MaxVolts": "5.0000", "528 Shutter-TriggerSequenceLength": "1000", "405 Shutter-Block voltage": "false", "XLIGHT Spin motor-State": "1", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "Vincent-D3-Time to open (ms)": "35.0000", "Vincent-D3-Time to close (ms)": "35.0000", "445 AO-TriggerSampleRate": "100000", "640 Shutter-IOChannel": "Dev1/ao14", "555 AO-Sequenceable": "1", "520 AO-Name": "AnalogIO", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "470 AO-Block voltage": "false", "COM1-Verbose": "1", "COM9-AnswerTimeout": "10000.0000", "528 Shutter-Name": "AnalogIO", "PRIME-Trigger Timeout (secs)": "10", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "NextFrame": 0, "470 AO-TriggerSequenceLength": "1000", "PRIME-TransposeXY": "0", "445 AO-TriggerSequenceLength": "1000", "COM1-Name": "COM1", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "COM9-DataBits": "8", "COM1-AnswerTimeout": "500.0000", "Camera": "", "TE2000-Version": "V2.50-V2.50-V2.10", "528 Shutter-Volts": "0.0000", "XLIGHT Hub-Name": "XLIGHT Hub", "PIEZO-Description": "Ludl stage driver adapter", "PositionName": "Pos3", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSampleRate": "100000", "PRIME-FanSpeedSetpoint": "High", "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "405 AO-TriggerSampleRate": "100000", "470 Shutter-Block voltage": "false", "470-State": "0", "COM3-Parity": "None", "405 AO-Volts": "4.5000", "PRIME-TransposeMirrorY": "0", "PRIME-ChipName": "GS144BSI", "470 AO-Volts": "0.0000", "PRIME-TransposeMirrorX": "0", "445 Shutter-SupportsTriggering": "0", "470 Shutter-SupportsTriggering": "0", "XLIGHT Hub-Description": "XLIGHT hub", "PRIME-PreampOffLimit": "10000", "470 AO-Sequenceable": "1", "528 Shutter-MaxVolts": "5.0000", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "Core-XYStage": "XYStage", "470 AO-TriggerSampleRate": "100000", "PRIME-Timing-PreTriggerDelayNs": "24672000.0000", "528-DA Device": "528 Shutter", "Focus-Description": "Nikon TE2000 focus adapter", "FilterBlock-State": "5", "520 AO-Demo": "No", "COM3-DelayBetweenCharsMs": "0.0000", "470 Shutter-Volts": "0.0000", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "PRIME-PP 2 THRESHOLD": "125", "PRIME-PP 4 THRESHOLD": "75", "XYStage-Acceleration": "4.0000", "470 Shutter-Demo": "No", "PRIME-Name": "PMPCIECam00", "640-DA Device": "640 Shutter", "405 AO-Block voltage": "false", "ElapsedTime-ms": 168813, "PRIME-Offset": "100", "445 AO-IOChannel": "Dev1/ao1", "640 AO-MaxVolts": "5.0000", "PositionIndex": 3, "PRIME-PP 1 ENABLED": "Yes", "COM1-BaudRate": "9600", "640-State": "0", "COM3-Verbose": "1", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "LudlController-TransmissionDelay": "0", "PRIME-FullWellCapacity": "62000", "PRIME-SMARTStreamingEnabled": "No", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "640 Shutter-Description": "NI DAC", "528-Description": "DA device that is used as a shutter", "COM4-Description": "Serial port driver (boost:asio)", "405 Shutter-Description": "NI DAC", "TE2000-Name": "TE2000", "528 Shutter-Description": "NI DAC", "COM9-Description": "Serial port driver (boost:asio)", "LudlController-Name": "LudlController", "445 Shutter-TriggerSequenceLength": "1000", "PRIME-SerialNumber": "A18B203008", "445 AO-Volts": "0.0000", "PRIME-X-dimension": "1200", "PRIME-ShutterCloseDelay": "0", "XLIGHT Disk slider-State": "2", "Position": "Default", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 AO-IOChannel": "Dev1/ao5", "520 AO-IOChannel": "Dev1/ao4", "528 AO-TriggerSequenceLength": "1000", "405 Shutter-MaxVolts": "5.0000", "470-DA Device": "470 Shutter", "PRIME-FTCapable": "No", "Core-ChannelGroup": "Channels", "555 AO-SupportsTriggering": "0", "PRIME-Y-dimension": "1200", "Frame": 0, "555 Shutter-Sequenceable": "1", "PRIME-Timing-ExposureTimeNs": "9992000.0000", "Core-ImageProcessor": "", "528-State": "0", "520 AO-Block voltage": "false", "XLIGHT Excitation Wheel-Label": "QUAD", "640 Shutter-Demo": "No", "COM3-StopBits": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "520 Shutter-IOChannel": "Dev1/ao11", "Width": 1200, "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-SupportsTriggering": "0", "640 AO-TriggerSampleRate": "100000", "520-Description": "DA device that is used as a shutter", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "405 Shutter-Sequenceable": "1", "405 Shutter-SupportsTriggering": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-TriggerSampleRate": "100000", "640 Shutter-MaxVolts": "5.0000", "555 AO-MaxVolts": "5.0000", "520 AO-TriggerSequenceLength": "1000", "COM3-BaudRate": "9600", "COM9-BaudRate": "9600", "Vincent-D3-Channel #": "Ch. #1", "PRIME-PMode": "Normal", "OpticalPath-Label": "Left(100)", "BitDepth": 16, "555 AO-TriggerSequenceLength": "1000", "445 Shutter-Description": "NI DAC", "405 AO-Sequenceable": "1", "PRIME-AcquisitionMethod": "Callbacks", "555 AO-Name": "AnalogIO", "PRIME-ShutterMode": "Pre-Exposure", "COM3-Handshaking": "Off", "445 AO-SupportsTriggering": "0", "ROI": "0-0-1200-1200", "COM9-Verbose": "1", "PRIME-Color": "OFF", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "PRIME-ShutterOpenDelay": "0", "COM1-Parity": "None", "Slice": 9, "SlicePosition": 40, "COM9-DelayBetweenCharsMs": "0.0000", "555-Name": "DA Shutter", "PIEZO-LudlSingleAxisName": "B", "XLIGHT Emission Wheel-Label": "QUAD", "640 Shutter-Name": "AnalogIO", "640 AO-Sequenceable": "1", "520 Shutter-Demo": "No", "528 Shutter-Demo": "No", "470-Name": "DA Shutter", "520-DA Device": "520 Shutter", "XYStage-Description": "Ludl XY stage driver adapter", "445 Shutter-MinVolts": "0.0000", "528 AO-TriggerSampleRate": "100000", "405 AO-MinVolts": "0.0000", "405 Shutter-TriggerSequenceLength": "1000", "Height": 1200, "555 AO-Block voltage": "false", "640 Shutter-Volts": "0.0000", "PixelType": "GRAY16", "445 Shutter-Block voltage": "false", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "520 Shutter-TriggerSequenceLength": "1000", "COM9-Name": "COM9", "Core-SLM": "", "555 AO-Volts": "0.0000", "555 Shutter-Block voltage": "false", "XYStage-Speed": "1800.0000", "520 AO-MaxVolts": "5.0000", "528 Shutter-SupportsTriggering": "0", "COM1-Description": "Serial port driver (boost:asio)", "PRIME-MetadataEnabled": "No", "520 Shutter-MinVolts": "0.0000", "470 Shutter-Sequenceable": "1", "PRIME-PreampDelay": "0", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-MinVolts": "0.0000", "ChannelIndex": 5, "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Core-AutoShutter": "1", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "Exposure-ms": 300, "PRIME-PP 2 ENABLED": "Yes", "640 Shutter-TriggerSampleRate": "100000", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "470 Shutter-IOChannel": "Dev1/ao10", "405 AO-Demo": "No", "PixelSizeUm": 1.1, "470 AO-Demo": "No", "470 Shutter-MaxVolts": "5.0000", "Vincent-D3-Name": "Vincent-D3", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FileName": "img_000000000_P-DAPI-high_009.tif", "LudlController-Reset": "Operate", "PRIME-Exposure": "300.00", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "445-Name": "DA Shutter", "555 Shutter-Name": "AnalogIO", "520 Shutter-TriggerSampleRate": "100000", "445 Shutter-IOChannel": "Dev1/ao9", "PRIME-TransposeCorrection": "0", "XYStage-StepSize": "0.1000", "445-Description": "DA device that is used as a shutter", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 AO-IOChannel": "Dev1/ao7", "520 Shutter-Name": "AnalogIO", "COM3-DataBits": "8", "640 AO-Volts": "0.0000", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "555 Shutter-IOChannel": "Dev1/ao13", "WaitInterval": null, "520 Shutter-Sequenceable": "1", "YPositionUm": 20690.300000000003, "445-State": "0", "445-DA Device": "445 Shutter", "PRIME-CircularBufferAutoSize": "ON", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "555 AO-TriggerSampleRate": "100000", "445 Shutter-Volts": "0.0000", "XLIGHT Hub-Port": "COM3", "Core-AutoFocus": "", "COM4-Handshaking": "Off", "528 Shutter-MinVolts": "0.0000", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "445 Shutter-Name": "AnalogIO", "528 Shutter-Sequenceable": "1", "XYStage-TransposeMirrorX": "0", "XYStage-TransposeMirrorY": "0", "PRIME-ReadoutRate": "100MHz 16bit", "XYStage-Name": "Stage", "640 AO-SupportsTriggering": "0", "640-Name": "DA Shutter", "640 Shutter-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "640 AO-Block voltage": "false", "445 Shutter-Sequenceable": "1", "470 AO-MaxVolts": "5.0000", "COM4-BaudRate": "9600", "470-Description": "DA device that is used as a shutter", "520 Shutter-Description": "NI DAC", "PRIME-CCDTemperature": "-14.9600", "Time": "2019-12-17 21:59:16 -0500", "640 AO-TriggerSequenceLength": "1000", "470 AO-Name": "AnalogIO", "XLIGHT Disk slider-Label": "Disk Pos 40um", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "Binning": "1x1", "TE2000-ModelType": "1", "COM3-AnswerTimeout": "500.0000", "555 Shutter-SupportsTriggering": "0", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "TE2000-Port": "COM4", "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Description": "NI DAC", "COM4-AnswerTimeout": "500.0000", "COM1-DelayBetweenCharsMs": "0.0000", "FilterBlock-Label": "Confocal", "445 AO-Name": "AnalogIO", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-Timing-ReadoutTimeNs": "24672000.0000", "528 AO-Demo": "No", "520 AO-Sequenceable": "1", "555-Description": "DA device that is used as a shutter", "405-DA Device": "405 Shutter", "405 AO-TriggerSequenceLength": "1000", "640 AO-MinVolts": "0.0000", "640 Shutter-Block voltage": "false", "FrameIndex": 0, "PRIME-Port": "CMOS", "555 AO-MinVolts": "0.0000", "COM3-Description": "Serial port driver (boost:asio)", "528 AO-Description": "NI DAC", "555 AO-IOChannel": "Dev1/ao6", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "555 AO-TriggerInputLine": "/Dev1/PFI0", "PRIME-ActualInterval-ms": "374.3700", "LudlController-Port": "COM9", "445 Shutter-Demo": "No", "PRIME-Trigger-Expose Out-Mux": "1", "SliceIndex": 9, "PRIME-CCDTemperatureSetPoint": "-15.0000", "405 Shutter-MinVolts": "0.0000", "PRIME-ExposeOutMode": "First Row", "AxisPositions": null, "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Volts": "0.0000", "528-Name": "DA Shutter", "555-State": "0", "XLIGHT Dichroic-Label": "QUAD", "445 AO-Sequenceable": "1", "405-Name": "DA Shutter", "405 AO-IOChannel": "Dev1/ao0", "Core-Camera": "PRIME", "Focus-Name": "Focus", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "405 Shutter-IOChannel": "Dev1/ao8", "640 AO-Demo": "No", "640 Shutter-SupportsTriggering": "0", "445 AO-Demo": "No", "555-DA Device": "555 Shutter", "470 Shutter-Description": "NI DAC", "528 AO-Volts": "0.0000", "COM1-DataBits": "8", "Vincent-D3-Address": "x", "520 AO-SupportsTriggering": "0", "405-Description": "DA device that is used as a shutter", "640 Shutter-MinVolts": "0.0000", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "405 AO-Name": "AnalogIO", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "640 AO-TriggerInputLine": "/Dev1/PFI0", "405 AO-TriggerInputLine": "/Dev1/PFI0", "640-Description": "DA device that is used as a shutter", "445 Shutter-TriggerSampleRate": "100000", "470 Shutter-TriggerSequenceLength": "1000", "COM1-StopBits": "1", "520 Shutter-SupportsTriggering": "0", "405-State": "1", "Core-Initialize": "1", "XLIGHT Emission Wheel-State": "7", "520 AO-Description": "NI DAC", "528 AO-TriggerInputLine": "/Dev1/PFI0", "445 Shutter-MaxVolts": "5.0000", "COM9-Handshaking": "Off", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "XLIGHT Spin motor-Label": "On (spinning)", "PRIME-PP 5 ENABLED": "No", "UUID": "5155ac3f-32a6-4558-88f5-523a86583797", "PRIME-TriggerMode": "Internal Trigger", "COM1-Handshaking": "Off", "PRIME-Timing-PostTriggerDelayNs": "49344000.0000", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "Focus-Position": "16479.8000", "528 Shutter-Block voltage": "false", "Channel": "P-DAPI-high", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "555 Shutter-Demo": "No", "405 Shutter-Demo": "No", "528 AO-Name": "AnalogIO", "520-State": "0", "Core-Galvo": "", "405 Shutter-TriggerSampleRate": "100000", "Focus-FocusStepSize_(nm)": "100", "PRIME-PixelType": "16bit", "COM4-DataBits": "8", "470 AO-IOChannel": "Dev1/ao2", "520 AO-MinVolts": "0.0000", "COM9-Parity": "None", "PRIME-CircularBufferEnabled": "ON", "PRIME-PP 6 Reset": "No", "640 AO-Description": "NI DAC", "Source": "PRIME", "445 AO-MinVolts": "0.0000", "PIEZO-Name": "Stage", "445 AO-Description": "NI DAC", "PRIME-CircularBufferFrameCount": "46", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "520 AO-Volts": "0.0000", "528 Shutter-TriggerSampleRate": "100000", "XPositionUm": 62321, "OpticalPath-State": "4", "PRIME-PP 3 THRESHOLD": "80", "PRIME-PVCAM Version": "3.7.1", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Gain": "1-HDR", "640 Shutter-TriggerSequenceLength": "1000", "528 AO-SupportsTriggering": "0", "445 AO-TriggerInputLine": "/Dev1/PFI0", "OpticalPath-Name": "OpticalPath", "PRIME-PP 4 ENABLED": "Yes", "PRIME-PVCAM Adapter Version": "1.3.29", "COM3-Name": "COM3", "405 AO-MaxVolts": "5.0000", "520 Shutter-Block voltage": "false", "PRIME-Timing-ClearingTimeNs": "49344000.0000", "Core-Focus": "PIEZO", "528 AO-Block voltage": "false", "TE2000-Description": "Nikon TE2000 hub", "528 AO-MinVolts": "0.0000", "COM4-Parity": "None", "Summary": { "Slices": 11, "ChContrastMin": [ 0, 0, 0, 0, 0, 0 ], "PixelAspect": 1, "SlicesFirst": true, "Interval_ms": 0, "Time": "2019-12-17 21:58:39 -0500", "Prefix": "seq_3", "InitialPositionList": [ { "Label": "Pos0", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.435], "Focus": [16480.2], "XYStage": [ 69822.40000000001, 20100.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos1", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.425], "Focus": [16480.3], "XYStage": [ 69004, 20101 ] }, "GridColumnIndex": 0 }, { "Label": "Pos2", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.44], "Focus": [16480.1], "XYStage": [ 63119.5, 20690.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos3", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.405], "Focus": [16480.2], "XYStage": [ 62321, 20690.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos4", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.415], "Focus": [16480], "XYStage": [ 56604.100000000006, 20688.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos5", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.42000000000002], "Focus": [16480.1], "XYStage": [ 56034.200000000004, 20688.800000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos6", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.455], "Focus": [16480], "XYStage": [ 50889.3, 20496.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos7", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.43], "Focus": [16480.2], "XYStage": [ 50101.600000000006, 20496.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos8", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.67000000000002], "Focus": [16479.9], "XYStage": [ 44818.600000000006, 20496.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos9", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.67000000000002], "Focus": [16480], "XYStage": [ 43897.9, 20436.800000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos10", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.675], "Focus": [16479.9], "XYStage": [ 39670.600000000006, 20664.9 ] }, "GridColumnIndex": 0 }, { "Label": "Pos11", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.69], "Focus": [16479.9], "XYStage": [ 38734, 20664.600000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos12", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.695], "Focus": [16480.1], "XYStage": [ 34037.200000000004, 20664.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos13", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.685], "Focus": [16480.1], "XYStage": [ 33152, 20664.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos14", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.69], "Focus": [16480.1], "XYStage": [ 28122.800000000003, 20664.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos15", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.675], "Focus": [16480], "XYStage": [ 27422.9, 20664.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos16", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.64000000000001], "Focus": [16480], "XYStage": [ 23005.2, 20664.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos17", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.69], "Focus": [16479.9], "XYStage": [ 21820.600000000002, 20409.600000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos18", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.41], "Focus": [16480.1], "XYStage": [ 73697.6, -13222.800000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos19", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.455], "Focus": [16480.1], "XYStage": [ 72765.8, -13223 ] }, "GridColumnIndex": 0 }, { "Label": "Pos20", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.4], "Focus": [16480.2], "XYStage": [ 67445.5, -12303.800000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos21", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.415], "Focus": [16480.2], "XYStage": [ 66613.3, -12303.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos22", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.415], "Focus": [16480.1], "XYStage": [ 61819.4, -11936.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos23", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.405], "Focus": [16480.2], "XYStage": [ 61325.700000000004, -11937.1 ] }, "GridColumnIndex": 0 }, { "Label": "Pos24", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.46], "Focus": [16480.2], "XYStage": [ 55377, -10974.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos25", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.445], "Focus": [16480.1], "XYStage": [ 54827, -11214.800000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos26", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.435], "Focus": [16480.2], "XYStage": [ 49937.100000000006, -10919.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos27", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.425], "Focus": [16480.1], "XYStage": [ 48764, -10919.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos28", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.42000000000002], "Focus": [16480.2], "XYStage": [ 43978.4, -10920.400000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos29", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.435], "Focus": [16480.2], "XYStage": [ 43106.3, -10920.400000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos30", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.405], "Focus": [16480.3], "XYStage": [ 38212.4, -10920.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos31", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.445], "Focus": [16480.2], "XYStage": [ 37254.9, -10920.400000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos32", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.44], "Focus": [16480.3], "XYStage": [ 32472.800000000003, -11294.400000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos33", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [166.95000000000002], "Focus": [16480.2], "XYStage": [ 31420.7, -11294.400000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos34", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [166.95000000000002], "Focus": [16480.2], "XYStage": [ 26881.5, -11640 ] }, "GridColumnIndex": 0 }, { "Label": "Pos35", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [166.98], "Focus": [16480.2], "XYStage": [ 25876.100000000002, -11641.1 ] }, "GridColumnIndex": 0 } ], "z-step_um": 10, "GridRow": 0, "BitDepth": 16, "Depth": 2, "Channels": 6, "Source": "Micro-Manager", "ChColors": [ -16711681, -256, -65281, -1, -1, -16776961 ], "MetadataVersion": 10, "KeepShutterOpenChannels": true, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536, 65536 ], "CustomIntervals_ms": [], "Height": 1200, "MicroManagerVersion": "1.4.23 20180219", "GridColumn": 0, "UUID": "8c8f9d20-b128-4577-9f91-89e70f22d319", "PixelType": "GRAY16", "Width": 1200, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\Yan\\20191217-YC113-S5S6", "Frames": 1, "Comment": "", "UserName": "Nikon", "PixelSize_um": 1.1, "ROI": [ 0, 0, 1200, 1200 ], "Date": "2019-12-17", "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC", "P-DAPI-high" ], "KeepShutterOpenSlices": true, "IJType": 1, "ComputerName": "ZadorConfocal2", "Positions": 36, "PositionIndex": 3, "CameraTimeout": 20000, "TimeFirst": false }, "640 AO-Name": "AnalogIO", "Vincent-D3-Command": "Close" }, "FrameKey-0-5-10": { "COM4-StopBits": "1", "528 AO-MaxVolts": "5.0000", "XLIGHT Excitation Wheel-State": "2", "470 Shutter-Name": "AnalogIO", "Core-Shutter": "405", "555 AO-Demo": "No", "XLIGHT Dichroic-State": "2", "XYStage-StepSize-Y": "0.1000", "ZPositionUm": 227.405, "XYStage-StepSize-X": "0.1000", "PRIME-FirmwareVersion": "20.28", "PRIME-ClearCycles": "2", "520-Name": "DA Shutter", "405 Shutter-Name": "AnalogIO", "PIEZO-StepSize": "0.0050", "PRIME-ClearMode": "Pre-Exposure", "470 AO-MinVolts": "0.0000", "405 AO-SupportsTriggering": "1", "CameraChannelIndex": 0, "XYStage-StartSpeed": "500.0000", "COM9-StopBits": "2", "405 AO-Description": "NI DAC", "COM4-DelayBetweenCharsMs": "0.0000", "445 AO-Block voltage": "false", "520 AO-TriggerInputLine": "/Dev1/PFI0", "555 AO-Description": "NI DAC", "520 AO-TriggerSampleRate": "100000", "COM4-Verbose": "1", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM4-Name": "COM4", "405 Shutter-Volts": "5.0000", "Vincent-D3-Port": "COM1", "PRIME-PP 4 MIN ADU AFFECTED": "200", "470 Shutter-MinVolts": "0.0000", "PRIME-PP 3 ENABLED": "Yes", "470 AO-Description": "NI DAC", "PRIME-Description": "PVCAM API device adapter", "520 Shutter-MaxVolts": "5.0000", "520 Shutter-Volts": "0.0000", "528 AO-Sequenceable": "1", "PRIME-Binning": "1x1", "555 Shutter-MaxVolts": "5.0000", "528 Shutter-TriggerSequenceLength": "1000", "405 Shutter-Block voltage": "false", "XLIGHT Spin motor-State": "1", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "Vincent-D3-Time to open (ms)": "35.0000", "Vincent-D3-Time to close (ms)": "35.0000", "445 AO-TriggerSampleRate": "100000", "640 Shutter-IOChannel": "Dev1/ao14", "555 AO-Sequenceable": "1", "520 AO-Name": "AnalogIO", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "470 AO-Block voltage": "false", "COM1-Verbose": "1", "COM9-AnswerTimeout": "10000.0000", "528 Shutter-Name": "AnalogIO", "PRIME-Trigger Timeout (secs)": "10", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "NextFrame": 0, "470 AO-TriggerSequenceLength": "1000", "PRIME-TransposeXY": "0", "445 AO-TriggerSequenceLength": "1000", "COM1-Name": "COM1", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "COM9-DataBits": "8", "COM1-AnswerTimeout": "500.0000", "Camera": "", "TE2000-Version": "V2.50-V2.50-V2.10", "528 Shutter-Volts": "0.0000", "XLIGHT Hub-Name": "XLIGHT Hub", "PIEZO-Description": "Ludl stage driver adapter", "PositionName": "Pos3", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSampleRate": "100000", "PRIME-FanSpeedSetpoint": "High", "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "405 AO-TriggerSampleRate": "100000", "470 Shutter-Block voltage": "false", "470-State": "0", "COM3-Parity": "None", "405 AO-Volts": "4.5000", "PRIME-TransposeMirrorY": "0", "PRIME-ChipName": "GS144BSI", "470 AO-Volts": "0.0000", "PRIME-TransposeMirrorX": "0", "445 Shutter-SupportsTriggering": "0", "470 Shutter-SupportsTriggering": "0", "XLIGHT Hub-Description": "XLIGHT hub", "PRIME-PreampOffLimit": "10000", "470 AO-Sequenceable": "1", "528 Shutter-MaxVolts": "5.0000", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "Core-XYStage": "XYStage", "470 AO-TriggerSampleRate": "100000", "PRIME-Timing-PreTriggerDelayNs": "24672000.0000", "528-DA Device": "528 Shutter", "Focus-Description": "Nikon TE2000 focus adapter", "FilterBlock-State": "5", "520 AO-Demo": "No", "COM3-DelayBetweenCharsMs": "0.0000", "470 Shutter-Volts": "0.0000", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "PRIME-PP 2 THRESHOLD": "125", "PRIME-PP 4 THRESHOLD": "75", "XYStage-Acceleration": "4.0000", "470 Shutter-Demo": "No", "PRIME-Name": "PMPCIECam00", "640-DA Device": "640 Shutter", "405 AO-Block voltage": "false", "ElapsedTime-ms": 169408, "PRIME-Offset": "100", "445 AO-IOChannel": "Dev1/ao1", "640 AO-MaxVolts": "5.0000", "PositionIndex": 3, "PRIME-PP 1 ENABLED": "Yes", "COM1-BaudRate": "9600", "640-State": "0", "COM3-Verbose": "1", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "LudlController-TransmissionDelay": "0", "PRIME-FullWellCapacity": "62000", "PRIME-SMARTStreamingEnabled": "No", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "640 Shutter-Description": "NI DAC", "528-Description": "DA device that is used as a shutter", "COM4-Description": "Serial port driver (boost:asio)", "405 Shutter-Description": "NI DAC", "TE2000-Name": "TE2000", "528 Shutter-Description": "NI DAC", "COM9-Description": "Serial port driver (boost:asio)", "LudlController-Name": "LudlController", "445 Shutter-TriggerSequenceLength": "1000", "PRIME-SerialNumber": "A18B203008", "445 AO-Volts": "0.0000", "PRIME-X-dimension": "1200", "PRIME-ShutterCloseDelay": "0", "XLIGHT Disk slider-State": "2", "Position": "Default", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 AO-IOChannel": "Dev1/ao5", "520 AO-IOChannel": "Dev1/ao4", "528 AO-TriggerSequenceLength": "1000", "405 Shutter-MaxVolts": "5.0000", "470-DA Device": "470 Shutter", "PRIME-FTCapable": "No", "Core-ChannelGroup": "Channels", "555 AO-SupportsTriggering": "0", "PRIME-Y-dimension": "1200", "Frame": 0, "555 Shutter-Sequenceable": "1", "PRIME-Timing-ExposureTimeNs": "9992000.0000", "Core-ImageProcessor": "", "528-State": "0", "520 AO-Block voltage": "false", "XLIGHT Excitation Wheel-Label": "QUAD", "640 Shutter-Demo": "No", "COM3-StopBits": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "520 Shutter-IOChannel": "Dev1/ao11", "Width": 1200, "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-SupportsTriggering": "0", "640 AO-TriggerSampleRate": "100000", "520-Description": "DA device that is used as a shutter", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "405 Shutter-Sequenceable": "1", "405 Shutter-SupportsTriggering": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-TriggerSampleRate": "100000", "640 Shutter-MaxVolts": "5.0000", "555 AO-MaxVolts": "5.0000", "520 AO-TriggerSequenceLength": "1000", "COM3-BaudRate": "9600", "COM9-BaudRate": "9600", "Vincent-D3-Channel #": "Ch. #1", "PRIME-PMode": "Normal", "OpticalPath-Label": "Left(100)", "BitDepth": 16, "555 AO-TriggerSequenceLength": "1000", "445 Shutter-Description": "NI DAC", "405 AO-Sequenceable": "1", "PRIME-AcquisitionMethod": "Callbacks", "555 AO-Name": "AnalogIO", "PRIME-ShutterMode": "Pre-Exposure", "COM3-Handshaking": "Off", "445 AO-SupportsTriggering": "0", "ROI": "0-0-1200-1200", "COM9-Verbose": "1", "PRIME-Color": "OFF", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "PRIME-ShutterOpenDelay": "0", "COM1-Parity": "None", "Slice": 10, "SlicePosition": 50, "COM9-DelayBetweenCharsMs": "0.0000", "555-Name": "DA Shutter", "PIEZO-LudlSingleAxisName": "B", "XLIGHT Emission Wheel-Label": "QUAD", "640 Shutter-Name": "AnalogIO", "640 AO-Sequenceable": "1", "520 Shutter-Demo": "No", "528 Shutter-Demo": "No", "470-Name": "DA Shutter", "520-DA Device": "520 Shutter", "XYStage-Description": "Ludl XY stage driver adapter", "445 Shutter-MinVolts": "0.0000", "528 AO-TriggerSampleRate": "100000", "405 AO-MinVolts": "0.0000", "405 Shutter-TriggerSequenceLength": "1000", "Height": 1200, "555 AO-Block voltage": "false", "640 Shutter-Volts": "0.0000", "PixelType": "GRAY16", "445 Shutter-Block voltage": "false", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "520 Shutter-TriggerSequenceLength": "1000", "COM9-Name": "COM9", "Core-SLM": "", "555 AO-Volts": "0.0000", "555 Shutter-Block voltage": "false", "XYStage-Speed": "1800.0000", "520 AO-MaxVolts": "5.0000", "528 Shutter-SupportsTriggering": "0", "COM1-Description": "Serial port driver (boost:asio)", "PRIME-MetadataEnabled": "No", "520 Shutter-MinVolts": "0.0000", "470 Shutter-Sequenceable": "1", "PRIME-PreampDelay": "0", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-MinVolts": "0.0000", "ChannelIndex": 5, "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Core-AutoShutter": "1", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "Exposure-ms": 300, "PRIME-PP 2 ENABLED": "Yes", "640 Shutter-TriggerSampleRate": "100000", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "470 Shutter-IOChannel": "Dev1/ao10", "405 AO-Demo": "No", "PixelSizeUm": 1.1, "470 AO-Demo": "No", "470 Shutter-MaxVolts": "5.0000", "Vincent-D3-Name": "Vincent-D3", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FileName": "img_000000000_P-DAPI-high_010.tif", "LudlController-Reset": "Operate", "PRIME-Exposure": "300.00", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "445-Name": "DA Shutter", "555 Shutter-Name": "AnalogIO", "520 Shutter-TriggerSampleRate": "100000", "445 Shutter-IOChannel": "Dev1/ao9", "PRIME-TransposeCorrection": "0", "XYStage-StepSize": "0.1000", "445-Description": "DA device that is used as a shutter", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 AO-IOChannel": "Dev1/ao7", "520 Shutter-Name": "AnalogIO", "COM3-DataBits": "8", "640 AO-Volts": "0.0000", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "555 Shutter-IOChannel": "Dev1/ao13", "WaitInterval": null, "520 Shutter-Sequenceable": "1", "YPositionUm": 20690.300000000003, "445-State": "0", "445-DA Device": "445 Shutter", "PRIME-CircularBufferAutoSize": "ON", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "555 AO-TriggerSampleRate": "100000", "445 Shutter-Volts": "0.0000", "XLIGHT Hub-Port": "COM3", "Core-AutoFocus": "", "COM4-Handshaking": "Off", "528 Shutter-MinVolts": "0.0000", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "445 Shutter-Name": "AnalogIO", "528 Shutter-Sequenceable": "1", "XYStage-TransposeMirrorX": "0", "XYStage-TransposeMirrorY": "0", "PRIME-ReadoutRate": "100MHz 16bit", "XYStage-Name": "Stage", "640 AO-SupportsTriggering": "0", "640-Name": "DA Shutter", "640 Shutter-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "640 AO-Block voltage": "false", "445 Shutter-Sequenceable": "1", "470 AO-MaxVolts": "5.0000", "COM4-BaudRate": "9600", "470-Description": "DA device that is used as a shutter", "520 Shutter-Description": "NI DAC", "PRIME-CCDTemperature": "-14.9600", "Time": "2019-12-17 21:59:16 -0500", "640 AO-TriggerSequenceLength": "1000", "470 AO-Name": "AnalogIO", "XLIGHT Disk slider-Label": "Disk Pos 40um", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "Binning": "1x1", "TE2000-ModelType": "1", "COM3-AnswerTimeout": "500.0000", "555 Shutter-SupportsTriggering": "0", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "TE2000-Port": "COM4", "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Description": "NI DAC", "COM4-AnswerTimeout": "500.0000", "COM1-DelayBetweenCharsMs": "0.0000", "FilterBlock-Label": "Confocal", "445 AO-Name": "AnalogIO", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-Timing-ReadoutTimeNs": "24672000.0000", "528 AO-Demo": "No", "520 AO-Sequenceable": "1", "555-Description": "DA device that is used as a shutter", "405-DA Device": "405 Shutter", "405 AO-TriggerSequenceLength": "1000", "640 AO-MinVolts": "0.0000", "640 Shutter-Block voltage": "false", "FrameIndex": 0, "PRIME-Port": "CMOS", "555 AO-MinVolts": "0.0000", "COM3-Description": "Serial port driver (boost:asio)", "528 AO-Description": "NI DAC", "555 AO-IOChannel": "Dev1/ao6", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "555 AO-TriggerInputLine": "/Dev1/PFI0", "PRIME-ActualInterval-ms": "374.3700", "LudlController-Port": "COM9", "445 Shutter-Demo": "No", "PRIME-Trigger-Expose Out-Mux": "1", "SliceIndex": 10, "PRIME-CCDTemperatureSetPoint": "-15.0000", "405 Shutter-MinVolts": "0.0000", "PRIME-ExposeOutMode": "First Row", "AxisPositions": null, "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Volts": "0.0000", "528-Name": "DA Shutter", "555-State": "0", "XLIGHT Dichroic-Label": "QUAD", "445 AO-Sequenceable": "1", "405-Name": "DA Shutter", "405 AO-IOChannel": "Dev1/ao0", "Core-Camera": "PRIME", "Focus-Name": "Focus", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "405 Shutter-IOChannel": "Dev1/ao8", "640 AO-Demo": "No", "640 Shutter-SupportsTriggering": "0", "445 AO-Demo": "No", "555-DA Device": "555 Shutter", "470 Shutter-Description": "NI DAC", "528 AO-Volts": "0.0000", "COM1-DataBits": "8", "Vincent-D3-Address": "x", "520 AO-SupportsTriggering": "0", "405-Description": "DA device that is used as a shutter", "640 Shutter-MinVolts": "0.0000", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "405 AO-Name": "AnalogIO", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "640 AO-TriggerInputLine": "/Dev1/PFI0", "405 AO-TriggerInputLine": "/Dev1/PFI0", "640-Description": "DA device that is used as a shutter", "445 Shutter-TriggerSampleRate": "100000", "470 Shutter-TriggerSequenceLength": "1000", "COM1-StopBits": "1", "520 Shutter-SupportsTriggering": "0", "405-State": "0", "Core-Initialize": "1", "XLIGHT Emission Wheel-State": "7", "520 AO-Description": "NI DAC", "528 AO-TriggerInputLine": "/Dev1/PFI0", "445 Shutter-MaxVolts": "5.0000", "COM9-Handshaking": "Off", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "XLIGHT Spin motor-Label": "On (spinning)", "PRIME-PP 5 ENABLED": "No", "UUID": "473988d7-cf5c-4cf9-b01d-2d3862eb99b9", "PRIME-TriggerMode": "Internal Trigger", "COM1-Handshaking": "Off", "PRIME-Timing-PostTriggerDelayNs": "49344000.0000", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "Focus-Position": "16479.8000", "528 Shutter-Block voltage": "false", "Channel": "P-DAPI-high", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "555 Shutter-Demo": "No", "405 Shutter-Demo": "No", "528 AO-Name": "AnalogIO", "520-State": "0", "Core-Galvo": "", "405 Shutter-TriggerSampleRate": "100000", "Focus-FocusStepSize_(nm)": "100", "PRIME-PixelType": "16bit", "COM4-DataBits": "8", "470 AO-IOChannel": "Dev1/ao2", "520 AO-MinVolts": "0.0000", "COM9-Parity": "None", "PRIME-CircularBufferEnabled": "ON", "PRIME-PP 6 Reset": "No", "640 AO-Description": "NI DAC", "Source": "PRIME", "445 AO-MinVolts": "0.0000", "PIEZO-Name": "Stage", "445 AO-Description": "NI DAC", "PRIME-CircularBufferFrameCount": "46", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "520 AO-Volts": "0.0000", "528 Shutter-TriggerSampleRate": "100000", "XPositionUm": 62321, "OpticalPath-State": "4", "PRIME-PP 3 THRESHOLD": "80", "PRIME-PVCAM Version": "3.7.1", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Gain": "1-HDR", "640 Shutter-TriggerSequenceLength": "1000", "528 AO-SupportsTriggering": "0", "445 AO-TriggerInputLine": "/Dev1/PFI0", "OpticalPath-Name": "OpticalPath", "PRIME-PP 4 ENABLED": "Yes", "PRIME-PVCAM Adapter Version": "1.3.29", "COM3-Name": "COM3", "405 AO-MaxVolts": "5.0000", "520 Shutter-Block voltage": "false", "PRIME-Timing-ClearingTimeNs": "49344000.0000", "Core-Focus": "PIEZO", "528 AO-Block voltage": "false", "TE2000-Description": "Nikon TE2000 hub", "528 AO-MinVolts": "0.0000", "COM4-Parity": "None", "Summary": { "Slices": 11, "ChContrastMin": [ 0, 0, 0, 0, 0, 0 ], "PixelAspect": 1, "SlicesFirst": true, "Interval_ms": 0, "Time": "2019-12-17 21:58:39 -0500", "Prefix": "seq_3", "InitialPositionList": [ { "Label": "Pos0", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.435], "Focus": [16480.2], "XYStage": [ 69822.40000000001, 20100.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos1", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.425], "Focus": [16480.3], "XYStage": [ 69004, 20101 ] }, "GridColumnIndex": 0 }, { "Label": "Pos2", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.44], "Focus": [16480.1], "XYStage": [ 63119.5, 20690.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos3", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.405], "Focus": [16480.2], "XYStage": [ 62321, 20690.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos4", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.415], "Focus": [16480], "XYStage": [ 56604.100000000006, 20688.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos5", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.42000000000002], "Focus": [16480.1], "XYStage": [ 56034.200000000004, 20688.800000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos6", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.455], "Focus": [16480], "XYStage": [ 50889.3, 20496.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos7", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.43], "Focus": [16480.2], "XYStage": [ 50101.600000000006, 20496.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos8", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.67000000000002], "Focus": [16479.9], "XYStage": [ 44818.600000000006, 20496.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos9", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.67000000000002], "Focus": [16480], "XYStage": [ 43897.9, 20436.800000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos10", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.675], "Focus": [16479.9], "XYStage": [ 39670.600000000006, 20664.9 ] }, "GridColumnIndex": 0 }, { "Label": "Pos11", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.69], "Focus": [16479.9], "XYStage": [ 38734, 20664.600000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos12", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.695], "Focus": [16480.1], "XYStage": [ 34037.200000000004, 20664.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos13", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.685], "Focus": [16480.1], "XYStage": [ 33152, 20664.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos14", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.69], "Focus": [16480.1], "XYStage": [ 28122.800000000003, 20664.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos15", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.675], "Focus": [16480], "XYStage": [ 27422.9, 20664.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos16", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.64000000000001], "Focus": [16480], "XYStage": [ 23005.2, 20664.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos17", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [197.69], "Focus": [16479.9], "XYStage": [ 21820.600000000002, 20409.600000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos18", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.41], "Focus": [16480.1], "XYStage": [ 73697.6, -13222.800000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos19", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.455], "Focus": [16480.1], "XYStage": [ 72765.8, -13223 ] }, "GridColumnIndex": 0 }, { "Label": "Pos20", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.4], "Focus": [16480.2], "XYStage": [ 67445.5, -12303.800000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos21", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.415], "Focus": [16480.2], "XYStage": [ 66613.3, -12303.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos22", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.415], "Focus": [16480.1], "XYStage": [ 61819.4, -11936.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos23", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.405], "Focus": [16480.2], "XYStage": [ 61325.700000000004, -11937.1 ] }, "GridColumnIndex": 0 }, { "Label": "Pos24", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.46], "Focus": [16480.2], "XYStage": [ 55377, -10974.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos25", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.445], "Focus": [16480.1], "XYStage": [ 54827, -11214.800000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos26", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.435], "Focus": [16480.2], "XYStage": [ 49937.100000000006, -10919.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos27", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.425], "Focus": [16480.1], "XYStage": [ 48764, -10919.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos28", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.42000000000002], "Focus": [16480.2], "XYStage": [ 43978.4, -10920.400000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos29", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.435], "Focus": [16480.2], "XYStage": [ 43106.3, -10920.400000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos30", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.405], "Focus": [16480.3], "XYStage": [ 38212.4, -10920.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos31", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.445], "Focus": [16480.2], "XYStage": [ 37254.9, -10920.400000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos32", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [177.44], "Focus": [16480.3], "XYStage": [ 32472.800000000003, -11294.400000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos33", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [166.95000000000002], "Focus": [16480.2], "XYStage": [ 31420.7, -11294.400000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos34", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [166.95000000000002], "Focus": [16480.2], "XYStage": [ 26881.5, -11640 ] }, "GridColumnIndex": 0 }, { "Label": "Pos35", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [166.98], "Focus": [16480.2], "XYStage": [ 25876.100000000002, -11641.1 ] }, "GridColumnIndex": 0 } ], "z-step_um": 10, "GridRow": 0, "BitDepth": 16, "Depth": 2, "Channels": 6, "Source": "Micro-Manager", "ChColors": [ -16711681, -256, -65281, -1, -1, -16776961 ], "MetadataVersion": 10, "KeepShutterOpenChannels": true, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536, 65536 ], "CustomIntervals_ms": [], "Height": 1200, "MicroManagerVersion": "1.4.23 20180219", "GridColumn": 0, "UUID": "8c8f9d20-b128-4577-9f91-89e70f22d319", "PixelType": "GRAY16", "Width": 1200, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\Yan\\20191217-YC113-S5S6", "Frames": 1, "Comment": "", "UserName": "Nikon", "PixelSize_um": 1.1, "ROI": [ 0, 0, 1200, 1200 ], "Date": "2019-12-17", "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC", "P-DAPI-high" ], "KeepShutterOpenSlices": true, "IJType": 1, "ComputerName": "ZadorConfocal2", "Positions": 36, "PositionIndex": 3, "CameraTimeout": 20000, "TimeFirst": false }, "640 AO-Name": "AnalogIO", "Vincent-D3-Command": "Close" } }