{ "Summary": { "Slices": 11, "ChContrastMin": [ 0, 0, 0, 0, 0, 0 ], "PixelAspect": 1, "SlicesFirst": true, "Interval_ms": 0, "Time": "2019-12-19 09:59:21 -0500", "Prefix": "seq_13", "InitialPositionList": [ { "Label": "Pos0", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.21], "Focus": [17741.5], "XYStage": [ 44542.200000000004, 31636.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos1", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.22], "Focus": [17741.4], "XYStage": [ 43801.100000000006, 31637.9 ] }, "GridColumnIndex": 0 }, { "Label": "Pos2", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.5], "XYStage": [ 38561.3, 32040.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos3", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.185], "Focus": [17741.3], "XYStage": [ 37698.4, 32041.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos4", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.16], "Focus": [17741.5], "XYStage": [ 32375.4, 31758.9 ] }, "GridColumnIndex": 0 }, { "Label": "Pos5", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.17000000000002], "Focus": [17741.3], "XYStage": [ 31770.7, 31758.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos6", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.4], "XYStage": [ 26536.800000000003, 31068.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos7", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.205], "Focus": [17741.4], "XYStage": [ 25420.2, 31068.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos8", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.85], "Focus": [17741.3], "XYStage": [ 21059, 31508 ] }, "GridColumnIndex": 0 }, { "Label": "Pos9", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.82], "Focus": [17741.3], "XYStage": [ 19731.9, 31506.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos10", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.79], "Focus": [17741.4], "XYStage": [ 14528.5, 32007.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos11", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.83], "Focus": [17741.2], "XYStage": [ 13830.300000000001, 32009.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos12", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.79], "Focus": [17741.3], "XYStage": [ 8775.300000000001, 32017.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos13", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.815], "Focus": [17741.3], "XYStage": [ 8109.200000000001, 32008.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos14", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.805], "Focus": [17741.3], "XYStage": [ 3483.3, 31908.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos15", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.82], "Focus": [17741.2], "XYStage": [ 2686.8, 31903.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos16", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [211.48000000000002], "Focus": [17741.1], "XYStage": [ -1978.9, 31973.600000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos17", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [211.51500000000001], "Focus": [17741.1], "XYStage": [ -2957.8, 31982.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos18", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.195], "Focus": [17741.5], "XYStage": [ 47747.4, -576.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos19", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.23000000000002], "Focus": [17741.6], "XYStage": [ 47053.9, -576.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos20", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.205], "Focus": [17741.4], "XYStage": [ 41871.200000000004, -479.8 ] }, "GridColumnIndex": 0 }, { "Label": "Pos21", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.4], "XYStage": [ 40882.600000000006, -392 ] }, "GridColumnIndex": 0 }, { "Label": "Pos22", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.24], "Focus": [17741.4], "XYStage": [ 35905.6, -135.3 ] }, "GridColumnIndex": 0 }, { "Label": "Pos23", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.195], "Focus": [17741.5], "XYStage": [ 34990.9, -132.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos24", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.46], "Focus": [17741.4], "XYStage": [ 29678.100000000002, -958.1 ] }, "GridColumnIndex": 0 }, { "Label": "Pos25", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.44], "Focus": [17741.5], "XYStage": [ 28983.600000000002, -939.4000000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos26", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.475], "Focus": [17741.5], "XYStage": [ 23019.7, -122 ] }, "GridColumnIndex": 0 }, { "Label": "Pos27", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.44], "Focus": [17741.7], "XYStage": [ 22428, -116.80000000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos28", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.46], "Focus": [17741.7], "XYStage": [ 16364.900000000001, -181.10000000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos29", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.48000000000002], "Focus": [17741.5], "XYStage": [ 15712.1, -180 ] }, "GridColumnIndex": 0 }, { "Label": "Pos30", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.45000000000002], "Focus": [17741.7], "XYStage": [ 10346.1, 77.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos31", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.49], "Focus": [17741.4], "XYStage": [ 9755.5, 85.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos32", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.475], "Focus": [17741.5], "XYStage": [ 4775.5, 293.90000000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos33", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.445], "Focus": [17741.4], "XYStage": [ 3954.9, 293.90000000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos34", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.455], "Focus": [17741.4], "XYStage": [ -1324.9, 545.6 ] }, "GridColumnIndex": 0 }, { "Label": "Pos35", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.445], "Focus": [17741.3], "XYStage": [ -2097, 576.6 ] }, "GridColumnIndex": 0 } ], "z-step_um": 10, "GridRow": 0, "BitDepth": 16, "Depth": 2, "Channels": 6, "Source": "Micro-Manager", "ChColors": [ -16711681, -256, -65281, -1, -1, -16776961 ], "MetadataVersion": 10, "KeepShutterOpenChannels": true, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536, 65536 ], "CustomIntervals_ms": [], "Height": 1200, "MicroManagerVersion": "1.4.23 20180219", "GridColumn": 0, "UUID": "18808e7c-f2e6-47b3-90d8-1d779ce87dbd", "PixelType": "GRAY16", "Width": 1200, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\Yan\\20191218-YC113-S7S8-miseq", "Frames": 1, "Comment": "", "UserName": "Nikon", "PixelSize_um": 1.1, "ROI": [ 0, 0, 1200, 1200 ], "Date": "2019-12-19", "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC", "P-DAPI-high" ], "KeepShutterOpenSlices": true, "IJType": 1, "ComputerName": "ZadorConfocal2", "Positions": 36, "PositionIndex": 34, "CameraTimeout": 20000, "TimeFirst": false }, "FrameKey-0-0-0": { "COM4-StopBits": "1", "528 AO-MaxVolts": "5.0000", "XLIGHT Excitation Wheel-State": "1", "470 Shutter-Name": "AnalogIO", "Core-Shutter": "520", "555 AO-Demo": "No", "XLIGHT Dichroic-State": "2", "XYStage-StepSize-Y": "0.1000", "ZPositionUm": 168.455, "XYStage-StepSize-X": "0.1000", "PRIME-FirmwareVersion": "20.28", "PRIME-ClearCycles": "2", "520-Name": "DA Shutter", "405 Shutter-Name": "AnalogIO", "PIEZO-StepSize": "0.0050", "PRIME-ClearMode": "Pre-Exposure", "470 AO-MinVolts": "0.0000", "405 AO-SupportsTriggering": "1", "CameraChannelIndex": 0, "XYStage-StartSpeed": "500.0000", "COM9-StopBits": "2", "405 AO-Description": "NI DAC", "COM4-DelayBetweenCharsMs": "0.0000", "445 AO-Block voltage": "false", "520 AO-TriggerInputLine": "/Dev1/PFI0", "555 AO-Description": "NI DAC", "520 AO-TriggerSampleRate": "100000", "COM4-Verbose": "1", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM4-Name": "COM4", "405 Shutter-Volts": "0.0000", "Vincent-D3-Port": "COM1", "PRIME-PP 4 MIN ADU AFFECTED": "200", "470 Shutter-MinVolts": "0.0000", "PRIME-PP 3 ENABLED": "Yes", "470 AO-Description": "NI DAC", "PRIME-Description": "PVCAM API device adapter", "520 Shutter-MaxVolts": "5.0000", "520 Shutter-Volts": "5.0000", "528 AO-Sequenceable": "1", "PRIME-Binning": "1x1", "555 Shutter-MaxVolts": "5.0000", "528 Shutter-TriggerSequenceLength": "1000", "405 Shutter-Block voltage": "false", "XLIGHT Spin motor-State": "1", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "Vincent-D3-Time to open (ms)": "35.0000", "Vincent-D3-Time to close (ms)": "35.0000", "445 AO-TriggerSampleRate": "100000", "640 Shutter-IOChannel": "Dev1/ao14", "555 AO-Sequenceable": "1", "520 AO-Name": "AnalogIO", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "470 AO-Block voltage": "false", "COM1-Verbose": "1", "COM9-AnswerTimeout": "10000.0000", "528 Shutter-Name": "AnalogIO", "PRIME-Trigger Timeout (secs)": "10", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "NextFrame": 0, "470 AO-TriggerSequenceLength": "1000", "PRIME-TransposeXY": "0", "445 AO-TriggerSequenceLength": "1000", "COM1-Name": "COM1", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "COM9-DataBits": "8", "COM1-AnswerTimeout": "500.0000", "Camera": "", "TE2000-Version": "V2.50-V2.50-V2.10", "528 Shutter-Volts": "0.0000", "XLIGHT Hub-Name": "XLIGHT Hub", "PIEZO-Description": "Ludl stage driver adapter", "PositionName": "Pos34", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSampleRate": "100000", "PRIME-FanSpeedSetpoint": "High", "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "405 AO-TriggerSampleRate": "100000", "470 Shutter-Block voltage": "false", "470-State": "0", "COM3-Parity": "None", "405 AO-Volts": "0.0000", "PRIME-TransposeMirrorY": "0", "PRIME-ChipName": "GS144BSI", "470 AO-Volts": "0.0000", "PRIME-TransposeMirrorX": "0", "445 Shutter-SupportsTriggering": "0", "470 Shutter-SupportsTriggering": "0", "XLIGHT Hub-Description": "XLIGHT hub", "PRIME-PreampOffLimit": "10000", "470 AO-Sequenceable": "1", "528 Shutter-MaxVolts": "5.0000", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "Core-XYStage": "XYStage", "470 AO-TriggerSampleRate": "100000", "PRIME-Timing-PreTriggerDelayNs": "24672000.0000", "528-DA Device": "528 Shutter", "Focus-Description": "Nikon TE2000 focus adapter", "FilterBlock-State": "5", "520 AO-Demo": "No", "COM3-DelayBetweenCharsMs": "0.0000", "470 Shutter-Volts": "0.0000", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "PRIME-PP 2 THRESHOLD": "125", "PRIME-PP 4 THRESHOLD": "75", "XYStage-Acceleration": "4.0000", "470 Shutter-Demo": "No", "PRIME-Name": "PMPCIECam00", "640-DA Device": "640 Shutter", "405 AO-Block voltage": "false", "ElapsedTime-ms": 1391060, "PRIME-Offset": "100", "445 AO-IOChannel": "Dev1/ao1", "640 AO-MaxVolts": "5.0000", "PositionIndex": 34, "PRIME-PP 1 ENABLED": "Yes", "COM1-BaudRate": "9600", "640-State": "0", "COM3-Verbose": "1", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "LudlController-TransmissionDelay": "0", "PRIME-FullWellCapacity": "62000", "PRIME-SMARTStreamingEnabled": "No", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "640 Shutter-Description": "NI DAC", "528-Description": "DA device that is used as a shutter", "COM4-Description": "Serial port driver (boost:asio)", "405 Shutter-Description": "NI DAC", "TE2000-Name": "TE2000", "528 Shutter-Description": "NI DAC", "COM9-Description": "Serial port driver (boost:asio)", "LudlController-Name": "LudlController", "445 Shutter-TriggerSequenceLength": "1000", "PRIME-SerialNumber": "A18B203008", "445 AO-Volts": "0.0000", "PRIME-X-dimension": "1200", "PRIME-ShutterCloseDelay": "0", "XLIGHT Disk slider-State": "2", "Position": "Default", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 AO-IOChannel": "Dev1/ao5", "520 AO-IOChannel": "Dev1/ao4", "528 AO-TriggerSequenceLength": "1000", "405 Shutter-MaxVolts": "5.0000", "470-DA Device": "470 Shutter", "PRIME-FTCapable": "No", "Core-ChannelGroup": "Channels", "555 AO-SupportsTriggering": "0", "PRIME-Y-dimension": "1200", "Frame": 0, "555 Shutter-Sequenceable": "1", "PRIME-Timing-ExposureTimeNs": "499998000.0000", "Core-ImageProcessor": "", "528-State": "0", "520 AO-Block voltage": "false", "XLIGHT Excitation Wheel-Label": "CFP/YFP", "640 Shutter-Demo": "No", "COM3-StopBits": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "520 Shutter-IOChannel": "Dev1/ao11", "Width": 1200, "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-SupportsTriggering": "0", "640 AO-TriggerSampleRate": "100000", "520-Description": "DA device that is used as a shutter", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "405 Shutter-Sequenceable": "1", "405 Shutter-SupportsTriggering": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-TriggerSampleRate": "100000", "640 Shutter-MaxVolts": "5.0000", "555 AO-MaxVolts": "5.0000", "520 AO-TriggerSequenceLength": "1000", "COM3-BaudRate": "9600", "COM9-BaudRate": "9600", "Vincent-D3-Channel #": "Ch. #1", "PRIME-PMode": "Normal", "OpticalPath-Label": "Left(100)", "BitDepth": 16, "555 AO-TriggerSequenceLength": "1000", "445 Shutter-Description": "NI DAC", "405 AO-Sequenceable": "1", "PRIME-AcquisitionMethod": "Callbacks", "555 AO-Name": "AnalogIO", "PRIME-ShutterMode": "Pre-Exposure", "COM3-Handshaking": "Off", "445 AO-SupportsTriggering": "0", "ROI": "0-0-1200-1200", "COM9-Verbose": "1", "PRIME-Color": "OFF", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "PRIME-ShutterOpenDelay": "0", "COM1-Parity": "None", "Slice": 0, "SlicePosition": -50, "COM9-DelayBetweenCharsMs": "0.0000", "555-Name": "DA Shutter", "PIEZO-LudlSingleAxisName": "B", "XLIGHT Emission Wheel-Label": "565/24(G)", "640 Shutter-Name": "AnalogIO", "640 AO-Sequenceable": "1", "520 Shutter-Demo": "No", "528 Shutter-Demo": "No", "470-Name": "DA Shutter", "520-DA Device": "520 Shutter", "XYStage-Description": "Ludl XY stage driver adapter", "445 Shutter-MinVolts": "0.0000", "528 AO-TriggerSampleRate": "100000", "405 AO-MinVolts": "0.0000", "405 Shutter-TriggerSequenceLength": "1000", "Height": 1200, "555 AO-Block voltage": "false", "640 Shutter-Volts": "0.0000", "PixelType": "GRAY16", "445 Shutter-Block voltage": "false", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "520 Shutter-TriggerSequenceLength": "1000", "COM9-Name": "COM9", "Core-SLM": "", "555 AO-Volts": "0.0000", "555 Shutter-Block voltage": "false", "XYStage-Speed": "1800.0000", "520 AO-MaxVolts": "5.0000", "528 Shutter-SupportsTriggering": "0", "COM1-Description": "Serial port driver (boost:asio)", "PRIME-MetadataEnabled": "No", "520 Shutter-MinVolts": "0.0000", "470 Shutter-Sequenceable": "1", "PRIME-PreampDelay": "0", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-MinVolts": "0.0000", "ChannelIndex": 0, "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Core-AutoShutter": "1", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "Exposure-ms": 320, "PRIME-PP 2 ENABLED": "Yes", "640 Shutter-TriggerSampleRate": "100000", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "470 Shutter-IOChannel": "Dev1/ao10", "405 AO-Demo": "No", "PixelSizeUm": 1.1, "470 AO-Demo": "No", "470 Shutter-MaxVolts": "5.0000", "Vincent-D3-Name": "Vincent-D3", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FileName": "img_000000000_Il-G_000.tif", "LudlController-Reset": "Operate", "PRIME-Exposure": "320.00", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "445-Name": "DA Shutter", "555 Shutter-Name": "AnalogIO", "520 Shutter-TriggerSampleRate": "100000", "445 Shutter-IOChannel": "Dev1/ao9", "PRIME-TransposeCorrection": "0", "XYStage-StepSize": "0.1000", "445-Description": "DA device that is used as a shutter", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 AO-IOChannel": "Dev1/ao7", "520 Shutter-Name": "AnalogIO", "COM3-DataBits": "8", "640 AO-Volts": "0.0000", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "555 Shutter-IOChannel": "Dev1/ao13", "WaitInterval": null, "520 Shutter-Sequenceable": "1", "YPositionUm": 545.6, "445-State": "0", "445-DA Device": "445 Shutter", "PRIME-CircularBufferAutoSize": "ON", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "555 AO-TriggerSampleRate": "100000", "445 Shutter-Volts": "0.0000", "XLIGHT Hub-Port": "COM3", "Core-AutoFocus": "", "COM4-Handshaking": "Off", "528 Shutter-MinVolts": "0.0000", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "445 Shutter-Name": "AnalogIO", "528 Shutter-Sequenceable": "1", "XYStage-TransposeMirrorX": "0", "XYStage-TransposeMirrorY": "0", "PRIME-ReadoutRate": "100MHz 16bit", "XYStage-Name": "Stage", "640 AO-SupportsTriggering": "0", "640-Name": "DA Shutter", "640 Shutter-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "640 AO-Block voltage": "false", "445 Shutter-Sequenceable": "1", "470 AO-MaxVolts": "5.0000", "COM4-BaudRate": "9600", "470-Description": "DA device that is used as a shutter", "520 Shutter-Description": "NI DAC", "PRIME-CCDTemperature": "-14.8500", "Time": "2019-12-19 09:59:21 -0500", "640 AO-TriggerSequenceLength": "1000", "470 AO-Name": "AnalogIO", "XLIGHT Disk slider-Label": "Disk Pos 40um", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "Binning": "1x1", "TE2000-ModelType": "1", "COM3-AnswerTimeout": "500.0000", "555 Shutter-SupportsTriggering": "0", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "TE2000-Port": "COM4", "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Description": "NI DAC", "COM4-AnswerTimeout": "500.0000", "COM1-DelayBetweenCharsMs": "0.0000", "FilterBlock-Label": "Confocal", "445 AO-Name": "AnalogIO", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-Timing-ReadoutTimeNs": "24672000.0000", "528 AO-Demo": "No", "520 AO-Sequenceable": "1", "555-Description": "DA device that is used as a shutter", "405-DA Device": "405 Shutter", "405 AO-TriggerSequenceLength": "1000", "640 AO-MinVolts": "0.0000", "640 Shutter-Block voltage": "false", "FrameIndex": 0, "PRIME-Port": "CMOS", "555 AO-MinVolts": "0.0000", "COM3-Description": "Serial port driver (boost:asio)", "528 AO-Description": "NI DAC", "555 AO-IOChannel": "Dev1/ao6", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "555 AO-TriggerInputLine": "/Dev1/PFI0", "PRIME-ActualInterval-ms": "390.5200", "LudlController-Port": "COM9", "445 Shutter-Demo": "No", "PRIME-Trigger-Expose Out-Mux": "1", "SliceIndex": 0, "PRIME-CCDTemperatureSetPoint": "-15.0000", "405 Shutter-MinVolts": "0.0000", "PRIME-ExposeOutMode": "First Row", "AxisPositions": null, "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Volts": "0.0000", "528-Name": "DA Shutter", "555-State": "0", "XLIGHT Dichroic-Label": "CFP/YFP", "445 AO-Sequenceable": "1", "405-Name": "DA Shutter", "405 AO-IOChannel": "Dev1/ao0", "Core-Camera": "PRIME", "Focus-Name": "Focus", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "405 Shutter-IOChannel": "Dev1/ao8", "640 AO-Demo": "No", "640 Shutter-SupportsTriggering": "0", "445 AO-Demo": "No", "555-DA Device": "555 Shutter", "470 Shutter-Description": "NI DAC", "528 AO-Volts": "0.0000", "COM1-DataBits": "8", "Vincent-D3-Address": "x", "520 AO-SupportsTriggering": "0", "405-Description": "DA device that is used as a shutter", "640 Shutter-MinVolts": "0.0000", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "405 AO-Name": "AnalogIO", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "640 AO-TriggerInputLine": "/Dev1/PFI0", "405 AO-TriggerInputLine": "/Dev1/PFI0", "640-Description": "DA device that is used as a shutter", "445 Shutter-TriggerSampleRate": "100000", "470 Shutter-TriggerSequenceLength": "1000", "COM1-StopBits": "1", "520 Shutter-SupportsTriggering": "0", "405-State": "0", "Core-Initialize": "1", "XLIGHT Emission Wheel-State": "3", "520 AO-Description": "NI DAC", "528 AO-TriggerInputLine": "/Dev1/PFI0", "445 Shutter-MaxVolts": "5.0000", "COM9-Handshaking": "Off", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "XLIGHT Spin motor-Label": "On (spinning)", "PRIME-PP 5 ENABLED": "No", "UUID": "42714352-61a0-4d4b-b09b-2a724d38830b", "PRIME-TriggerMode": "Internal Trigger", "COM1-Handshaking": "Off", "PRIME-Timing-PostTriggerDelayNs": "49344000.0000", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "Focus-Position": "17741.5000", "528 Shutter-Block voltage": "false", "Channel": "Il-G", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "555 Shutter-Demo": "No", "405 Shutter-Demo": "No", "528 AO-Name": "AnalogIO", "520-State": "1", "Core-Galvo": "", "405 Shutter-TriggerSampleRate": "100000", "Focus-FocusStepSize_(nm)": "100", "PRIME-PixelType": "16bit", "COM4-DataBits": "8", "470 AO-IOChannel": "Dev1/ao2", "520 AO-MinVolts": "0.0000", "COM9-Parity": "None", "PRIME-CircularBufferEnabled": "ON", "PRIME-PP 6 Reset": "No", "640 AO-Description": "NI DAC", "Source": "PRIME", "445 AO-MinVolts": "0.0000", "PIEZO-Name": "Stage", "445 AO-Description": "NI DAC", "PRIME-CircularBufferFrameCount": "46", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "520 AO-Volts": "4.5000", "528 Shutter-TriggerSampleRate": "100000", "XPositionUm": -1324.9, "OpticalPath-State": "4", "PRIME-PP 3 THRESHOLD": "80", "PRIME-PVCAM Version": "3.7.1", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Gain": "1-HDR", "640 Shutter-TriggerSequenceLength": "1000", "528 AO-SupportsTriggering": "0", "445 AO-TriggerInputLine": "/Dev1/PFI0", "OpticalPath-Name": "OpticalPath", "PRIME-PP 4 ENABLED": "Yes", "PRIME-PVCAM Adapter Version": "1.3.29", "COM3-Name": "COM3", "405 AO-MaxVolts": "5.0000", "520 Shutter-Block voltage": "false", "PRIME-Timing-ClearingTimeNs": "49344000.0000", "Core-Focus": "PIEZO", "528 AO-Block voltage": "false", "TE2000-Description": "Nikon TE2000 hub", "528 AO-MinVolts": "0.0000", "COM4-Parity": "None", "Summary": { "Slices": 11, "ChContrastMin": [ 0, 0, 0, 0, 0, 0 ], "PixelAspect": 1, "SlicesFirst": true, "Interval_ms": 0, "Time": "2019-12-19 09:59:21 -0500", "Prefix": "seq_13", "InitialPositionList": [ { "Label": "Pos0", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.21], "Focus": [17741.5], "XYStage": [ 44542.200000000004, 31636.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos1", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.22], "Focus": [17741.4], "XYStage": [ 43801.100000000006, 31637.9 ] }, "GridColumnIndex": 0 }, { "Label": "Pos2", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.5], "XYStage": [ 38561.3, 32040.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos3", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.185], "Focus": [17741.3], "XYStage": [ 37698.4, 32041.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos4", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.16], "Focus": [17741.5], "XYStage": [ 32375.4, 31758.9 ] }, "GridColumnIndex": 0 }, { "Label": "Pos5", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.17000000000002], "Focus": [17741.3], "XYStage": [ 31770.7, 31758.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos6", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.4], "XYStage": [ 26536.800000000003, 31068.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos7", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.205], "Focus": [17741.4], "XYStage": [ 25420.2, 31068.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos8", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.85], "Focus": [17741.3], "XYStage": [ 21059, 31508 ] }, "GridColumnIndex": 0 }, { "Label": "Pos9", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.82], "Focus": [17741.3], "XYStage": [ 19731.9, 31506.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos10", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.79], "Focus": [17741.4], "XYStage": [ 14528.5, 32007.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos11", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.83], "Focus": [17741.2], "XYStage": [ 13830.300000000001, 32009.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos12", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.79], "Focus": [17741.3], "XYStage": [ 8775.300000000001, 32017.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos13", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.815], "Focus": [17741.3], "XYStage": [ 8109.200000000001, 32008.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos14", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.805], "Focus": [17741.3], "XYStage": [ 3483.3, 31908.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos15", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.82], "Focus": [17741.2], "XYStage": [ 2686.8, 31903.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos16", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [211.48000000000002], "Focus": [17741.1], "XYStage": [ -1978.9, 31973.600000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos17", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [211.51500000000001], "Focus": [17741.1], "XYStage": [ -2957.8, 31982.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos18", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.195], "Focus": [17741.5], "XYStage": [ 47747.4, -576.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos19", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.23000000000002], "Focus": [17741.6], "XYStage": [ 47053.9, -576.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos20", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.205], "Focus": [17741.4], "XYStage": [ 41871.200000000004, -479.8 ] }, "GridColumnIndex": 0 }, { "Label": "Pos21", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.4], "XYStage": [ 40882.600000000006, -392 ] }, "GridColumnIndex": 0 }, { "Label": "Pos22", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.24], "Focus": [17741.4], "XYStage": [ 35905.6, -135.3 ] }, "GridColumnIndex": 0 }, { "Label": "Pos23", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.195], "Focus": [17741.5], "XYStage": [ 34990.9, -132.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos24", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.46], "Focus": [17741.4], "XYStage": [ 29678.100000000002, -958.1 ] }, "GridColumnIndex": 0 }, { "Label": "Pos25", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.44], "Focus": [17741.5], "XYStage": [ 28983.600000000002, -939.4000000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos26", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.475], "Focus": [17741.5], "XYStage": [ 23019.7, -122 ] }, "GridColumnIndex": 0 }, { "Label": "Pos27", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.44], "Focus": [17741.7], "XYStage": [ 22428, -116.80000000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos28", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.46], "Focus": [17741.7], "XYStage": [ 16364.900000000001, -181.10000000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos29", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.48000000000002], "Focus": [17741.5], "XYStage": [ 15712.1, -180 ] }, "GridColumnIndex": 0 }, { "Label": "Pos30", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.45000000000002], "Focus": [17741.7], "XYStage": [ 10346.1, 77.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos31", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.49], "Focus": [17741.4], "XYStage": [ 9755.5, 85.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos32", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.475], "Focus": [17741.5], "XYStage": [ 4775.5, 293.90000000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos33", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.445], "Focus": [17741.4], "XYStage": [ 3954.9, 293.90000000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos34", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.455], "Focus": [17741.4], "XYStage": [ -1324.9, 545.6 ] }, "GridColumnIndex": 0 }, { "Label": "Pos35", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.445], "Focus": [17741.3], "XYStage": [ -2097, 576.6 ] }, "GridColumnIndex": 0 } ], "z-step_um": 10, "GridRow": 0, "BitDepth": 16, "Depth": 2, "Channels": 6, "Source": "Micro-Manager", "ChColors": [ -16711681, -256, -65281, -1, -1, -16776961 ], "MetadataVersion": 10, "KeepShutterOpenChannels": true, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536, 65536 ], "CustomIntervals_ms": [], "Height": 1200, "MicroManagerVersion": "1.4.23 20180219", "GridColumn": 0, "UUID": "18808e7c-f2e6-47b3-90d8-1d779ce87dbd", "PixelType": "GRAY16", "Width": 1200, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\Yan\\20191218-YC113-S7S8-miseq", "Frames": 1, "Comment": "", "UserName": "Nikon", "PixelSize_um": 1.1, "ROI": [ 0, 0, 1200, 1200 ], "Date": "2019-12-19", "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC", "P-DAPI-high" ], "KeepShutterOpenSlices": true, "IJType": 1, "ComputerName": "ZadorConfocal2", "Positions": 36, "PositionIndex": 34, "CameraTimeout": 20000, "TimeFirst": false }, "640 AO-Name": "AnalogIO", "Vincent-D3-Command": "Close" }, "FrameKey-0-0-1": { "COM4-StopBits": "1", "528 AO-MaxVolts": "5.0000", "XLIGHT Excitation Wheel-State": "1", "470 Shutter-Name": "AnalogIO", "Core-Shutter": "520", "555 AO-Demo": "No", "XLIGHT Dichroic-State": "2", "XYStage-StepSize-Y": "0.1000", "ZPositionUm": 178.455, "XYStage-StepSize-X": "0.1000", "PRIME-FirmwareVersion": "20.28", "PRIME-ClearCycles": "2", "520-Name": "DA Shutter", "405 Shutter-Name": "AnalogIO", "PIEZO-StepSize": "0.0050", "PRIME-ClearMode": "Pre-Exposure", "470 AO-MinVolts": "0.0000", "405 AO-SupportsTriggering": "1", "CameraChannelIndex": 0, "XYStage-StartSpeed": "500.0000", "COM9-StopBits": "2", "405 AO-Description": "NI DAC", "COM4-DelayBetweenCharsMs": "0.0000", "445 AO-Block voltage": "false", "520 AO-TriggerInputLine": "/Dev1/PFI0", "555 AO-Description": "NI DAC", "520 AO-TriggerSampleRate": "100000", "COM4-Verbose": "1", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM4-Name": "COM4", "405 Shutter-Volts": "0.0000", "Vincent-D3-Port": "COM1", "PRIME-PP 4 MIN ADU AFFECTED": "200", "470 Shutter-MinVolts": "0.0000", "PRIME-PP 3 ENABLED": "Yes", "470 AO-Description": "NI DAC", "PRIME-Description": "PVCAM API device adapter", "520 Shutter-MaxVolts": "5.0000", "520 Shutter-Volts": "5.0000", "528 AO-Sequenceable": "1", "PRIME-Binning": "1x1", "555 Shutter-MaxVolts": "5.0000", "528 Shutter-TriggerSequenceLength": "1000", "405 Shutter-Block voltage": "false", "XLIGHT Spin motor-State": "1", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "Vincent-D3-Time to open (ms)": "35.0000", "Vincent-D3-Time to close (ms)": "35.0000", "445 AO-TriggerSampleRate": "100000", "640 Shutter-IOChannel": "Dev1/ao14", "555 AO-Sequenceable": "1", "520 AO-Name": "AnalogIO", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "470 AO-Block voltage": "false", "COM1-Verbose": "1", "COM9-AnswerTimeout": "10000.0000", "528 Shutter-Name": "AnalogIO", "PRIME-Trigger Timeout (secs)": "10", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "NextFrame": 0, "470 AO-TriggerSequenceLength": "1000", "PRIME-TransposeXY": "0", "445 AO-TriggerSequenceLength": "1000", "COM1-Name": "COM1", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "COM9-DataBits": "8", "COM1-AnswerTimeout": "500.0000", "Camera": "", "TE2000-Version": "V2.50-V2.50-V2.10", "528 Shutter-Volts": "0.0000", "XLIGHT Hub-Name": "XLIGHT Hub", "PIEZO-Description": "Ludl stage driver adapter", "PositionName": "Pos34", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSampleRate": "100000", "PRIME-FanSpeedSetpoint": "High", "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "405 AO-TriggerSampleRate": "100000", "470 Shutter-Block voltage": "false", "470-State": "0", "COM3-Parity": "None", "405 AO-Volts": "0.0000", "PRIME-TransposeMirrorY": "0", "PRIME-ChipName": "GS144BSI", "470 AO-Volts": "0.0000", "PRIME-TransposeMirrorX": "0", "445 Shutter-SupportsTriggering": "0", "470 Shutter-SupportsTriggering": "0", "XLIGHT Hub-Description": "XLIGHT hub", "PRIME-PreampOffLimit": "10000", "470 AO-Sequenceable": "1", "528 Shutter-MaxVolts": "5.0000", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "Core-XYStage": "XYStage", "470 AO-TriggerSampleRate": "100000", "PRIME-Timing-PreTriggerDelayNs": "24672000.0000", "528-DA Device": "528 Shutter", "Focus-Description": "Nikon TE2000 focus adapter", "FilterBlock-State": "5", "520 AO-Demo": "No", "COM3-DelayBetweenCharsMs": "0.0000", "470 Shutter-Volts": "0.0000", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "PRIME-PP 2 THRESHOLD": "125", "PRIME-PP 4 THRESHOLD": "75", "XYStage-Acceleration": "4.0000", "470 Shutter-Demo": "No", "PRIME-Name": "PMPCIECam00", "640-DA Device": "640 Shutter", "405 AO-Block voltage": "false", "ElapsedTime-ms": 1391625, "PRIME-Offset": "100", "445 AO-IOChannel": "Dev1/ao1", "640 AO-MaxVolts": "5.0000", "PositionIndex": 34, "PRIME-PP 1 ENABLED": "Yes", "COM1-BaudRate": "9600", "640-State": "0", "COM3-Verbose": "1", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "LudlController-TransmissionDelay": "0", "PRIME-FullWellCapacity": "62000", "PRIME-SMARTStreamingEnabled": "No", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "640 Shutter-Description": "NI DAC", "528-Description": "DA device that is used as a shutter", "COM4-Description": "Serial port driver (boost:asio)", "405 Shutter-Description": "NI DAC", "TE2000-Name": "TE2000", "528 Shutter-Description": "NI DAC", "COM9-Description": "Serial port driver (boost:asio)", "LudlController-Name": "LudlController", "445 Shutter-TriggerSequenceLength": "1000", "PRIME-SerialNumber": "A18B203008", "445 AO-Volts": "0.0000", "PRIME-X-dimension": "1200", "PRIME-ShutterCloseDelay": "0", "XLIGHT Disk slider-State": "2", "Position": "Default", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 AO-IOChannel": "Dev1/ao5", "520 AO-IOChannel": "Dev1/ao4", "528 AO-TriggerSequenceLength": "1000", "405 Shutter-MaxVolts": "5.0000", "470-DA Device": "470 Shutter", "PRIME-FTCapable": "No", "Core-ChannelGroup": "Channels", "555 AO-SupportsTriggering": "0", "PRIME-Y-dimension": "1200", "Frame": 0, "555 Shutter-Sequenceable": "1", "PRIME-Timing-ExposureTimeNs": "499998000.0000", "Core-ImageProcessor": "", "528-State": "0", "520 AO-Block voltage": "false", "XLIGHT Excitation Wheel-Label": "CFP/YFP", "640 Shutter-Demo": "No", "COM3-StopBits": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "520 Shutter-IOChannel": "Dev1/ao11", "Width": 1200, "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-SupportsTriggering": "0", "640 AO-TriggerSampleRate": "100000", "520-Description": "DA device that is used as a shutter", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "405 Shutter-Sequenceable": "1", "405 Shutter-SupportsTriggering": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-TriggerSampleRate": "100000", "640 Shutter-MaxVolts": "5.0000", "555 AO-MaxVolts": "5.0000", "520 AO-TriggerSequenceLength": "1000", "COM3-BaudRate": "9600", "COM9-BaudRate": "9600", "Vincent-D3-Channel #": "Ch. #1", "PRIME-PMode": "Normal", "OpticalPath-Label": "Left(100)", "BitDepth": 16, "555 AO-TriggerSequenceLength": "1000", "445 Shutter-Description": "NI DAC", "405 AO-Sequenceable": "1", "PRIME-AcquisitionMethod": "Callbacks", "555 AO-Name": "AnalogIO", "PRIME-ShutterMode": "Pre-Exposure", "COM3-Handshaking": "Off", "445 AO-SupportsTriggering": "0", "ROI": "0-0-1200-1200", "COM9-Verbose": "1", "PRIME-Color": "OFF", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "PRIME-ShutterOpenDelay": "0", "COM1-Parity": "None", "Slice": 1, "SlicePosition": -40, "COM9-DelayBetweenCharsMs": "0.0000", "555-Name": "DA Shutter", "PIEZO-LudlSingleAxisName": "B", "XLIGHT Emission Wheel-Label": "565/24(G)", "640 Shutter-Name": "AnalogIO", "640 AO-Sequenceable": "1", "520 Shutter-Demo": "No", "528 Shutter-Demo": "No", "470-Name": "DA Shutter", "520-DA Device": "520 Shutter", "XYStage-Description": "Ludl XY stage driver adapter", "445 Shutter-MinVolts": "0.0000", "528 AO-TriggerSampleRate": "100000", "405 AO-MinVolts": "0.0000", "405 Shutter-TriggerSequenceLength": "1000", "Height": 1200, "555 AO-Block voltage": "false", "640 Shutter-Volts": "0.0000", "PixelType": "GRAY16", "445 Shutter-Block voltage": "false", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "520 Shutter-TriggerSequenceLength": "1000", "COM9-Name": "COM9", "Core-SLM": "", "555 AO-Volts": "0.0000", "555 Shutter-Block voltage": "false", "XYStage-Speed": "1800.0000", "520 AO-MaxVolts": "5.0000", "528 Shutter-SupportsTriggering": "0", "COM1-Description": "Serial port driver (boost:asio)", "PRIME-MetadataEnabled": "No", "520 Shutter-MinVolts": "0.0000", "470 Shutter-Sequenceable": "1", "PRIME-PreampDelay": "0", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-MinVolts": "0.0000", "ChannelIndex": 0, "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Core-AutoShutter": "1", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "Exposure-ms": 320, "PRIME-PP 2 ENABLED": "Yes", "640 Shutter-TriggerSampleRate": "100000", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "470 Shutter-IOChannel": "Dev1/ao10", "405 AO-Demo": "No", "PixelSizeUm": 1.1, "470 AO-Demo": "No", "470 Shutter-MaxVolts": "5.0000", "Vincent-D3-Name": "Vincent-D3", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FileName": "img_000000000_Il-G_001.tif", "LudlController-Reset": "Operate", "PRIME-Exposure": "320.00", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "445-Name": "DA Shutter", "555 Shutter-Name": "AnalogIO", "520 Shutter-TriggerSampleRate": "100000", "445 Shutter-IOChannel": "Dev1/ao9", "PRIME-TransposeCorrection": "0", "XYStage-StepSize": "0.1000", "445-Description": "DA device that is used as a shutter", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 AO-IOChannel": "Dev1/ao7", "520 Shutter-Name": "AnalogIO", "COM3-DataBits": "8", "640 AO-Volts": "0.0000", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "555 Shutter-IOChannel": "Dev1/ao13", "WaitInterval": null, "520 Shutter-Sequenceable": "1", "YPositionUm": 545.6, "445-State": "0", "445-DA Device": "445 Shutter", "PRIME-CircularBufferAutoSize": "ON", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "555 AO-TriggerSampleRate": "100000", "445 Shutter-Volts": "0.0000", "XLIGHT Hub-Port": "COM3", "Core-AutoFocus": "", "COM4-Handshaking": "Off", "528 Shutter-MinVolts": "0.0000", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "445 Shutter-Name": "AnalogIO", "528 Shutter-Sequenceable": "1", "XYStage-TransposeMirrorX": "0", "XYStage-TransposeMirrorY": "0", "PRIME-ReadoutRate": "100MHz 16bit", "XYStage-Name": "Stage", "640 AO-SupportsTriggering": "0", "640-Name": "DA Shutter", "640 Shutter-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "640 AO-Block voltage": "false", "445 Shutter-Sequenceable": "1", "470 AO-MaxVolts": "5.0000", "COM4-BaudRate": "9600", "470-Description": "DA device that is used as a shutter", "520 Shutter-Description": "NI DAC", "PRIME-CCDTemperature": "-14.8500", "Time": "2019-12-19 09:59:22 -0500", "640 AO-TriggerSequenceLength": "1000", "470 AO-Name": "AnalogIO", "XLIGHT Disk slider-Label": "Disk Pos 40um", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "Binning": "1x1", "TE2000-ModelType": "1", "COM3-AnswerTimeout": "500.0000", "555 Shutter-SupportsTriggering": "0", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "TE2000-Port": "COM4", "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Description": "NI DAC", "COM4-AnswerTimeout": "500.0000", "COM1-DelayBetweenCharsMs": "0.0000", "FilterBlock-Label": "Confocal", "445 AO-Name": "AnalogIO", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-Timing-ReadoutTimeNs": "24672000.0000", "528 AO-Demo": "No", "520 AO-Sequenceable": "1", "555-Description": "DA device that is used as a shutter", "405-DA Device": "405 Shutter", "405 AO-TriggerSequenceLength": "1000", "640 AO-MinVolts": "0.0000", "640 Shutter-Block voltage": "false", "FrameIndex": 0, "PRIME-Port": "CMOS", "555 AO-MinVolts": "0.0000", "COM3-Description": "Serial port driver (boost:asio)", "528 AO-Description": "NI DAC", "555 AO-IOChannel": "Dev1/ao6", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "555 AO-TriggerInputLine": "/Dev1/PFI0", "PRIME-ActualInterval-ms": "390.5200", "LudlController-Port": "COM9", "445 Shutter-Demo": "No", "PRIME-Trigger-Expose Out-Mux": "1", "SliceIndex": 1, "PRIME-CCDTemperatureSetPoint": "-15.0000", "405 Shutter-MinVolts": "0.0000", "PRIME-ExposeOutMode": "First Row", "AxisPositions": null, "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Volts": "0.0000", "528-Name": "DA Shutter", "555-State": "0", "XLIGHT Dichroic-Label": "CFP/YFP", "445 AO-Sequenceable": "1", "405-Name": "DA Shutter", "405 AO-IOChannel": "Dev1/ao0", "Core-Camera": "PRIME", "Focus-Name": "Focus", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "405 Shutter-IOChannel": "Dev1/ao8", "640 AO-Demo": "No", "640 Shutter-SupportsTriggering": "0", "445 AO-Demo": "No", "555-DA Device": "555 Shutter", "470 Shutter-Description": "NI DAC", "528 AO-Volts": "0.0000", "COM1-DataBits": "8", "Vincent-D3-Address": "x", "520 AO-SupportsTriggering": "0", "405-Description": "DA device that is used as a shutter", "640 Shutter-MinVolts": "0.0000", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "405 AO-Name": "AnalogIO", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "640 AO-TriggerInputLine": "/Dev1/PFI0", "405 AO-TriggerInputLine": "/Dev1/PFI0", "640-Description": "DA device that is used as a shutter", "445 Shutter-TriggerSampleRate": "100000", "470 Shutter-TriggerSequenceLength": "1000", "COM1-StopBits": "1", "520 Shutter-SupportsTriggering": "0", "405-State": "0", "Core-Initialize": "1", "XLIGHT Emission Wheel-State": "3", "520 AO-Description": "NI DAC", "528 AO-TriggerInputLine": "/Dev1/PFI0", "445 Shutter-MaxVolts": "5.0000", "COM9-Handshaking": "Off", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "XLIGHT Spin motor-Label": "On (spinning)", "PRIME-PP 5 ENABLED": "No", "UUID": "77c0443b-7047-4b52-ae4b-5dc42bd0f6d3", "PRIME-TriggerMode": "Internal Trigger", "COM1-Handshaking": "Off", "PRIME-Timing-PostTriggerDelayNs": "49344000.0000", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "Focus-Position": "17741.5000", "528 Shutter-Block voltage": "false", "Channel": "Il-G", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "555 Shutter-Demo": "No", "405 Shutter-Demo": "No", "528 AO-Name": "AnalogIO", "520-State": "1", "Core-Galvo": "", "405 Shutter-TriggerSampleRate": "100000", "Focus-FocusStepSize_(nm)": "100", "PRIME-PixelType": "16bit", "COM4-DataBits": "8", "470 AO-IOChannel": "Dev1/ao2", "520 AO-MinVolts": "0.0000", "COM9-Parity": "None", "PRIME-CircularBufferEnabled": "ON", "PRIME-PP 6 Reset": "No", "640 AO-Description": "NI DAC", "Source": "PRIME", "445 AO-MinVolts": "0.0000", "PIEZO-Name": "Stage", "445 AO-Description": "NI DAC", "PRIME-CircularBufferFrameCount": "46", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "520 AO-Volts": "4.5000", "528 Shutter-TriggerSampleRate": "100000", "XPositionUm": -1324.9, "OpticalPath-State": "4", "PRIME-PP 3 THRESHOLD": "80", "PRIME-PVCAM Version": "3.7.1", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Gain": "1-HDR", "640 Shutter-TriggerSequenceLength": "1000", "528 AO-SupportsTriggering": "0", "445 AO-TriggerInputLine": "/Dev1/PFI0", "OpticalPath-Name": "OpticalPath", "PRIME-PP 4 ENABLED": "Yes", "PRIME-PVCAM Adapter Version": "1.3.29", "COM3-Name": "COM3", "405 AO-MaxVolts": "5.0000", "520 Shutter-Block voltage": "false", "PRIME-Timing-ClearingTimeNs": "49344000.0000", "Core-Focus": "PIEZO", "528 AO-Block voltage": "false", "TE2000-Description": "Nikon TE2000 hub", "528 AO-MinVolts": "0.0000", "COM4-Parity": "None", "Summary": { "Slices": 11, "ChContrastMin": [ 0, 0, 0, 0, 0, 0 ], "PixelAspect": 1, "SlicesFirst": true, "Interval_ms": 0, "Time": "2019-12-19 09:59:21 -0500", "Prefix": "seq_13", "InitialPositionList": [ { "Label": "Pos0", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.21], "Focus": [17741.5], "XYStage": [ 44542.200000000004, 31636.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos1", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.22], "Focus": [17741.4], "XYStage": [ 43801.100000000006, 31637.9 ] }, "GridColumnIndex": 0 }, { "Label": "Pos2", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.5], "XYStage": [ 38561.3, 32040.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos3", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.185], "Focus": [17741.3], "XYStage": [ 37698.4, 32041.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos4", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.16], "Focus": [17741.5], "XYStage": [ 32375.4, 31758.9 ] }, "GridColumnIndex": 0 }, { "Label": "Pos5", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.17000000000002], "Focus": [17741.3], "XYStage": [ 31770.7, 31758.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos6", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.4], "XYStage": [ 26536.800000000003, 31068.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos7", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.205], "Focus": [17741.4], "XYStage": [ 25420.2, 31068.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos8", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.85], "Focus": [17741.3], "XYStage": [ 21059, 31508 ] }, "GridColumnIndex": 0 }, { "Label": "Pos9", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.82], "Focus": [17741.3], "XYStage": [ 19731.9, 31506.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos10", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.79], "Focus": [17741.4], "XYStage": [ 14528.5, 32007.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos11", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.83], "Focus": [17741.2], "XYStage": [ 13830.300000000001, 32009.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos12", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.79], "Focus": [17741.3], "XYStage": [ 8775.300000000001, 32017.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos13", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.815], "Focus": [17741.3], "XYStage": [ 8109.200000000001, 32008.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos14", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.805], "Focus": [17741.3], "XYStage": [ 3483.3, 31908.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos15", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.82], "Focus": [17741.2], "XYStage": [ 2686.8, 31903.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos16", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [211.48000000000002], "Focus": [17741.1], "XYStage": [ -1978.9, 31973.600000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos17", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [211.51500000000001], "Focus": [17741.1], "XYStage": [ -2957.8, 31982.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos18", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.195], "Focus": [17741.5], "XYStage": [ 47747.4, -576.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos19", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.23000000000002], "Focus": [17741.6], "XYStage": [ 47053.9, -576.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos20", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.205], "Focus": [17741.4], "XYStage": [ 41871.200000000004, -479.8 ] }, "GridColumnIndex": 0 }, { "Label": "Pos21", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.4], "XYStage": [ 40882.600000000006, -392 ] }, "GridColumnIndex": 0 }, { "Label": "Pos22", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.24], "Focus": [17741.4], "XYStage": [ 35905.6, -135.3 ] }, "GridColumnIndex": 0 }, { "Label": "Pos23", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.195], "Focus": [17741.5], "XYStage": [ 34990.9, -132.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos24", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.46], "Focus": [17741.4], "XYStage": [ 29678.100000000002, -958.1 ] }, "GridColumnIndex": 0 }, { "Label": "Pos25", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.44], "Focus": [17741.5], "XYStage": [ 28983.600000000002, -939.4000000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos26", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.475], "Focus": [17741.5], "XYStage": [ 23019.7, -122 ] }, "GridColumnIndex": 0 }, { "Label": "Pos27", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.44], "Focus": [17741.7], "XYStage": [ 22428, -116.80000000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos28", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.46], "Focus": [17741.7], "XYStage": [ 16364.900000000001, -181.10000000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos29", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.48000000000002], "Focus": [17741.5], "XYStage": [ 15712.1, -180 ] }, "GridColumnIndex": 0 }, { "Label": "Pos30", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.45000000000002], "Focus": [17741.7], "XYStage": [ 10346.1, 77.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos31", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.49], "Focus": [17741.4], "XYStage": [ 9755.5, 85.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos32", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.475], "Focus": [17741.5], "XYStage": [ 4775.5, 293.90000000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos33", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.445], "Focus": [17741.4], "XYStage": [ 3954.9, 293.90000000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos34", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.455], "Focus": [17741.4], "XYStage": [ -1324.9, 545.6 ] }, "GridColumnIndex": 0 }, { "Label": "Pos35", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.445], "Focus": [17741.3], "XYStage": [ -2097, 576.6 ] }, "GridColumnIndex": 0 } ], "z-step_um": 10, "GridRow": 0, "BitDepth": 16, "Depth": 2, "Channels": 6, "Source": "Micro-Manager", "ChColors": [ -16711681, -256, -65281, -1, -1, -16776961 ], "MetadataVersion": 10, "KeepShutterOpenChannels": true, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536, 65536 ], "CustomIntervals_ms": [], "Height": 1200, "MicroManagerVersion": "1.4.23 20180219", "GridColumn": 0, "UUID": "18808e7c-f2e6-47b3-90d8-1d779ce87dbd", "PixelType": "GRAY16", "Width": 1200, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\Yan\\20191218-YC113-S7S8-miseq", "Frames": 1, "Comment": "", "UserName": "Nikon", "PixelSize_um": 1.1, "ROI": [ 0, 0, 1200, 1200 ], "Date": "2019-12-19", "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC", "P-DAPI-high" ], "KeepShutterOpenSlices": true, "IJType": 1, "ComputerName": "ZadorConfocal2", "Positions": 36, "PositionIndex": 34, "CameraTimeout": 20000, "TimeFirst": false }, "640 AO-Name": "AnalogIO", "Vincent-D3-Command": "Close" }, "FrameKey-0-0-2": { "COM4-StopBits": "1", "528 AO-MaxVolts": "5.0000", "XLIGHT Excitation Wheel-State": "1", "470 Shutter-Name": "AnalogIO", "Core-Shutter": "520", "555 AO-Demo": "No", "XLIGHT Dichroic-State": "2", "XYStage-StepSize-Y": "0.1000", "ZPositionUm": 188.455, "XYStage-StepSize-X": "0.1000", "PRIME-FirmwareVersion": "20.28", "PRIME-ClearCycles": "2", "520-Name": "DA Shutter", "405 Shutter-Name": "AnalogIO", "PIEZO-StepSize": "0.0050", "PRIME-ClearMode": "Pre-Exposure", "470 AO-MinVolts": "0.0000", "405 AO-SupportsTriggering": "1", "CameraChannelIndex": 0, "XYStage-StartSpeed": "500.0000", "COM9-StopBits": "2", "405 AO-Description": "NI DAC", "COM4-DelayBetweenCharsMs": "0.0000", "445 AO-Block voltage": "false", "520 AO-TriggerInputLine": "/Dev1/PFI0", "555 AO-Description": "NI DAC", "520 AO-TriggerSampleRate": "100000", "COM4-Verbose": "1", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM4-Name": "COM4", "405 Shutter-Volts": "0.0000", "Vincent-D3-Port": "COM1", "PRIME-PP 4 MIN ADU AFFECTED": "200", "470 Shutter-MinVolts": "0.0000", "PRIME-PP 3 ENABLED": "Yes", "470 AO-Description": "NI DAC", "PRIME-Description": "PVCAM API device adapter", "520 Shutter-MaxVolts": "5.0000", "520 Shutter-Volts": "5.0000", "528 AO-Sequenceable": "1", "PRIME-Binning": "1x1", "555 Shutter-MaxVolts": "5.0000", "528 Shutter-TriggerSequenceLength": "1000", "405 Shutter-Block voltage": "false", "XLIGHT Spin motor-State": "1", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "Vincent-D3-Time to open (ms)": "35.0000", "Vincent-D3-Time to close (ms)": "35.0000", "445 AO-TriggerSampleRate": "100000", "640 Shutter-IOChannel": "Dev1/ao14", "555 AO-Sequenceable": "1", "520 AO-Name": "AnalogIO", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "470 AO-Block voltage": "false", "COM1-Verbose": "1", "COM9-AnswerTimeout": "10000.0000", "528 Shutter-Name": "AnalogIO", "PRIME-Trigger Timeout (secs)": "10", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "NextFrame": 0, "470 AO-TriggerSequenceLength": "1000", "PRIME-TransposeXY": "0", "445 AO-TriggerSequenceLength": "1000", "COM1-Name": "COM1", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "COM9-DataBits": "8", "COM1-AnswerTimeout": "500.0000", "Camera": "", "TE2000-Version": "V2.50-V2.50-V2.10", "528 Shutter-Volts": "0.0000", "XLIGHT Hub-Name": "XLIGHT Hub", "PIEZO-Description": "Ludl stage driver adapter", "PositionName": "Pos34", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSampleRate": "100000", "PRIME-FanSpeedSetpoint": "High", "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "405 AO-TriggerSampleRate": "100000", "470 Shutter-Block voltage": "false", "470-State": "0", "COM3-Parity": "None", "405 AO-Volts": "0.0000", "PRIME-TransposeMirrorY": "0", "PRIME-ChipName": "GS144BSI", "470 AO-Volts": "0.0000", "PRIME-TransposeMirrorX": "0", "445 Shutter-SupportsTriggering": "0", "470 Shutter-SupportsTriggering": "0", "XLIGHT Hub-Description": "XLIGHT hub", "PRIME-PreampOffLimit": "10000", "470 AO-Sequenceable": "1", "528 Shutter-MaxVolts": "5.0000", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "Core-XYStage": "XYStage", "470 AO-TriggerSampleRate": "100000", "PRIME-Timing-PreTriggerDelayNs": "24672000.0000", "528-DA Device": "528 Shutter", "Focus-Description": "Nikon TE2000 focus adapter", "FilterBlock-State": "5", "520 AO-Demo": "No", "COM3-DelayBetweenCharsMs": "0.0000", "470 Shutter-Volts": "0.0000", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "PRIME-PP 2 THRESHOLD": "125", "PRIME-PP 4 THRESHOLD": "75", "XYStage-Acceleration": "4.0000", "470 Shutter-Demo": "No", "PRIME-Name": "PMPCIECam00", "640-DA Device": "640 Shutter", "405 AO-Block voltage": "false", "ElapsedTime-ms": 1392241, "PRIME-Offset": "100", "445 AO-IOChannel": "Dev1/ao1", "640 AO-MaxVolts": "5.0000", "PositionIndex": 34, "PRIME-PP 1 ENABLED": "Yes", "COM1-BaudRate": "9600", "640-State": "0", "COM3-Verbose": "1", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "LudlController-TransmissionDelay": "0", "PRIME-FullWellCapacity": "62000", "PRIME-SMARTStreamingEnabled": "No", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "640 Shutter-Description": "NI DAC", "528-Description": "DA device that is used as a shutter", "COM4-Description": "Serial port driver (boost:asio)", "405 Shutter-Description": "NI DAC", "TE2000-Name": "TE2000", "528 Shutter-Description": "NI DAC", "COM9-Description": "Serial port driver (boost:asio)", "LudlController-Name": "LudlController", "445 Shutter-TriggerSequenceLength": "1000", "PRIME-SerialNumber": "A18B203008", "445 AO-Volts": "0.0000", "PRIME-X-dimension": "1200", "PRIME-ShutterCloseDelay": "0", "XLIGHT Disk slider-State": "2", "Position": "Default", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 AO-IOChannel": "Dev1/ao5", "520 AO-IOChannel": "Dev1/ao4", "528 AO-TriggerSequenceLength": "1000", "405 Shutter-MaxVolts": "5.0000", "470-DA Device": "470 Shutter", "PRIME-FTCapable": "No", "Core-ChannelGroup": "Channels", "555 AO-SupportsTriggering": "0", "PRIME-Y-dimension": "1200", "Frame": 0, "555 Shutter-Sequenceable": "1", "PRIME-Timing-ExposureTimeNs": "499998000.0000", "Core-ImageProcessor": "", "528-State": "0", "520 AO-Block voltage": "false", "XLIGHT Excitation Wheel-Label": "CFP/YFP", "640 Shutter-Demo": "No", "COM3-StopBits": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "520 Shutter-IOChannel": "Dev1/ao11", "Width": 1200, "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-SupportsTriggering": "0", "640 AO-TriggerSampleRate": "100000", "520-Description": "DA device that is used as a shutter", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "405 Shutter-Sequenceable": "1", "405 Shutter-SupportsTriggering": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-TriggerSampleRate": "100000", "640 Shutter-MaxVolts": "5.0000", "555 AO-MaxVolts": "5.0000", "520 AO-TriggerSequenceLength": "1000", "COM3-BaudRate": "9600", "COM9-BaudRate": "9600", "Vincent-D3-Channel #": "Ch. #1", "PRIME-PMode": "Normal", "OpticalPath-Label": "Left(100)", "BitDepth": 16, "555 AO-TriggerSequenceLength": "1000", "445 Shutter-Description": "NI DAC", "405 AO-Sequenceable": "1", "PRIME-AcquisitionMethod": "Callbacks", "555 AO-Name": "AnalogIO", "PRIME-ShutterMode": "Pre-Exposure", "COM3-Handshaking": "Off", "445 AO-SupportsTriggering": "0", "ROI": "0-0-1200-1200", "COM9-Verbose": "1", "PRIME-Color": "OFF", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "PRIME-ShutterOpenDelay": "0", "COM1-Parity": "None", "Slice": 2, "SlicePosition": -30, "COM9-DelayBetweenCharsMs": "0.0000", "555-Name": "DA Shutter", "PIEZO-LudlSingleAxisName": "B", "XLIGHT Emission Wheel-Label": "565/24(G)", "640 Shutter-Name": "AnalogIO", "640 AO-Sequenceable": "1", "520 Shutter-Demo": "No", "528 Shutter-Demo": "No", "470-Name": "DA Shutter", "520-DA Device": "520 Shutter", "XYStage-Description": "Ludl XY stage driver adapter", "445 Shutter-MinVolts": "0.0000", "528 AO-TriggerSampleRate": "100000", "405 AO-MinVolts": "0.0000", "405 Shutter-TriggerSequenceLength": "1000", "Height": 1200, "555 AO-Block voltage": "false", "640 Shutter-Volts": "0.0000", "PixelType": "GRAY16", "445 Shutter-Block voltage": "false", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "520 Shutter-TriggerSequenceLength": "1000", "COM9-Name": "COM9", "Core-SLM": "", "555 AO-Volts": "0.0000", "555 Shutter-Block voltage": "false", "XYStage-Speed": "1800.0000", "520 AO-MaxVolts": "5.0000", "528 Shutter-SupportsTriggering": "0", "COM1-Description": "Serial port driver (boost:asio)", "PRIME-MetadataEnabled": "No", "520 Shutter-MinVolts": "0.0000", "470 Shutter-Sequenceable": "1", "PRIME-PreampDelay": "0", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-MinVolts": "0.0000", "ChannelIndex": 0, "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Core-AutoShutter": "1", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "Exposure-ms": 320, "PRIME-PP 2 ENABLED": "Yes", "640 Shutter-TriggerSampleRate": "100000", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "470 Shutter-IOChannel": "Dev1/ao10", "405 AO-Demo": "No", "PixelSizeUm": 1.1, "470 AO-Demo": "No", "470 Shutter-MaxVolts": "5.0000", "Vincent-D3-Name": "Vincent-D3", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FileName": "img_000000000_Il-G_002.tif", "LudlController-Reset": "Operate", "PRIME-Exposure": "320.00", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "445-Name": "DA Shutter", "555 Shutter-Name": "AnalogIO", "520 Shutter-TriggerSampleRate": "100000", "445 Shutter-IOChannel": "Dev1/ao9", "PRIME-TransposeCorrection": "0", "XYStage-StepSize": "0.1000", "445-Description": "DA device that is used as a shutter", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 AO-IOChannel": "Dev1/ao7", "520 Shutter-Name": "AnalogIO", "COM3-DataBits": "8", "640 AO-Volts": "0.0000", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "555 Shutter-IOChannel": "Dev1/ao13", "WaitInterval": null, "520 Shutter-Sequenceable": "1", "YPositionUm": 545.6, "445-State": "0", "445-DA Device": "445 Shutter", "PRIME-CircularBufferAutoSize": "ON", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "555 AO-TriggerSampleRate": "100000", "445 Shutter-Volts": "0.0000", "XLIGHT Hub-Port": "COM3", "Core-AutoFocus": "", "COM4-Handshaking": "Off", "528 Shutter-MinVolts": "0.0000", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "445 Shutter-Name": "AnalogIO", "528 Shutter-Sequenceable": "1", "XYStage-TransposeMirrorX": "0", "XYStage-TransposeMirrorY": "0", "PRIME-ReadoutRate": "100MHz 16bit", "XYStage-Name": "Stage", "640 AO-SupportsTriggering": "0", "640-Name": "DA Shutter", "640 Shutter-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "640 AO-Block voltage": "false", "445 Shutter-Sequenceable": "1", "470 AO-MaxVolts": "5.0000", "COM4-BaudRate": "9600", "470-Description": "DA device that is used as a shutter", "520 Shutter-Description": "NI DAC", "PRIME-CCDTemperature": "-14.8500", "Time": "2019-12-19 09:59:22 -0500", "640 AO-TriggerSequenceLength": "1000", "470 AO-Name": "AnalogIO", "XLIGHT Disk slider-Label": "Disk Pos 40um", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "Binning": "1x1", "TE2000-ModelType": "1", "COM3-AnswerTimeout": "500.0000", "555 Shutter-SupportsTriggering": "0", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "TE2000-Port": "COM4", "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Description": "NI DAC", "COM4-AnswerTimeout": "500.0000", "COM1-DelayBetweenCharsMs": "0.0000", "FilterBlock-Label": "Confocal", "445 AO-Name": "AnalogIO", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-Timing-ReadoutTimeNs": "24672000.0000", "528 AO-Demo": "No", "520 AO-Sequenceable": "1", "555-Description": "DA device that is used as a shutter", "405-DA Device": "405 Shutter", "405 AO-TriggerSequenceLength": "1000", "640 AO-MinVolts": "0.0000", "640 Shutter-Block voltage": "false", "FrameIndex": 0, "PRIME-Port": "CMOS", "555 AO-MinVolts": "0.0000", "COM3-Description": "Serial port driver (boost:asio)", "528 AO-Description": "NI DAC", "555 AO-IOChannel": "Dev1/ao6", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "555 AO-TriggerInputLine": "/Dev1/PFI0", "PRIME-ActualInterval-ms": "390.5200", "LudlController-Port": "COM9", "445 Shutter-Demo": "No", "PRIME-Trigger-Expose Out-Mux": "1", "SliceIndex": 2, "PRIME-CCDTemperatureSetPoint": "-15.0000", "405 Shutter-MinVolts": "0.0000", "PRIME-ExposeOutMode": "First Row", "AxisPositions": null, "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Volts": "0.0000", "528-Name": "DA Shutter", "555-State": "0", "XLIGHT Dichroic-Label": "CFP/YFP", "445 AO-Sequenceable": "1", "405-Name": "DA Shutter", "405 AO-IOChannel": "Dev1/ao0", "Core-Camera": "PRIME", "Focus-Name": "Focus", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "405 Shutter-IOChannel": "Dev1/ao8", "640 AO-Demo": "No", "640 Shutter-SupportsTriggering": "0", "445 AO-Demo": "No", "555-DA Device": "555 Shutter", "470 Shutter-Description": "NI DAC", "528 AO-Volts": "0.0000", "COM1-DataBits": "8", "Vincent-D3-Address": "x", "520 AO-SupportsTriggering": "0", "405-Description": "DA device that is used as a shutter", "640 Shutter-MinVolts": "0.0000", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "405 AO-Name": "AnalogIO", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "640 AO-TriggerInputLine": "/Dev1/PFI0", "405 AO-TriggerInputLine": "/Dev1/PFI0", "640-Description": "DA device that is used as a shutter", "445 Shutter-TriggerSampleRate": "100000", "470 Shutter-TriggerSequenceLength": "1000", "COM1-StopBits": "1", "520 Shutter-SupportsTriggering": "0", "405-State": "0", "Core-Initialize": "1", "XLIGHT Emission Wheel-State": "3", "520 AO-Description": "NI DAC", "528 AO-TriggerInputLine": "/Dev1/PFI0", "445 Shutter-MaxVolts": "5.0000", "COM9-Handshaking": "Off", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "XLIGHT Spin motor-Label": "On (spinning)", "PRIME-PP 5 ENABLED": "No", "UUID": "52fa84b2-6572-468a-aefa-97b55de0dfb8", "PRIME-TriggerMode": "Internal Trigger", "COM1-Handshaking": "Off", "PRIME-Timing-PostTriggerDelayNs": "49344000.0000", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "Focus-Position": "17741.5000", "528 Shutter-Block voltage": "false", "Channel": "Il-G", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "555 Shutter-Demo": "No", "405 Shutter-Demo": "No", "528 AO-Name": "AnalogIO", "520-State": "1", "Core-Galvo": "", "405 Shutter-TriggerSampleRate": "100000", "Focus-FocusStepSize_(nm)": "100", "PRIME-PixelType": "16bit", "COM4-DataBits": "8", "470 AO-IOChannel": "Dev1/ao2", "520 AO-MinVolts": "0.0000", "COM9-Parity": "None", "PRIME-CircularBufferEnabled": "ON", "PRIME-PP 6 Reset": "No", "640 AO-Description": "NI DAC", "Source": "PRIME", "445 AO-MinVolts": "0.0000", "PIEZO-Name": "Stage", "445 AO-Description": "NI DAC", "PRIME-CircularBufferFrameCount": "46", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "520 AO-Volts": "4.5000", "528 Shutter-TriggerSampleRate": "100000", "XPositionUm": -1324.9, "OpticalPath-State": "4", "PRIME-PP 3 THRESHOLD": "80", "PRIME-PVCAM Version": "3.7.1", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Gain": "1-HDR", "640 Shutter-TriggerSequenceLength": "1000", "528 AO-SupportsTriggering": "0", "445 AO-TriggerInputLine": "/Dev1/PFI0", "OpticalPath-Name": "OpticalPath", "PRIME-PP 4 ENABLED": "Yes", "PRIME-PVCAM Adapter Version": "1.3.29", "COM3-Name": "COM3", "405 AO-MaxVolts": "5.0000", "520 Shutter-Block voltage": "false", "PRIME-Timing-ClearingTimeNs": "49344000.0000", "Core-Focus": "PIEZO", "528 AO-Block voltage": "false", "TE2000-Description": "Nikon TE2000 hub", "528 AO-MinVolts": "0.0000", "COM4-Parity": "None", "Summary": { "Slices": 11, "ChContrastMin": [ 0, 0, 0, 0, 0, 0 ], "PixelAspect": 1, "SlicesFirst": true, "Interval_ms": 0, "Time": "2019-12-19 09:59:21 -0500", "Prefix": "seq_13", "InitialPositionList": [ { "Label": "Pos0", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.21], "Focus": [17741.5], "XYStage": [ 44542.200000000004, 31636.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos1", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.22], "Focus": [17741.4], "XYStage": [ 43801.100000000006, 31637.9 ] }, "GridColumnIndex": 0 }, { "Label": "Pos2", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.5], "XYStage": [ 38561.3, 32040.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos3", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.185], "Focus": [17741.3], "XYStage": [ 37698.4, 32041.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos4", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.16], "Focus": [17741.5], "XYStage": [ 32375.4, 31758.9 ] }, "GridColumnIndex": 0 }, { "Label": "Pos5", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.17000000000002], "Focus": [17741.3], "XYStage": [ 31770.7, 31758.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos6", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.4], "XYStage": [ 26536.800000000003, 31068.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos7", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.205], "Focus": [17741.4], "XYStage": [ 25420.2, 31068.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos8", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.85], "Focus": [17741.3], "XYStage": [ 21059, 31508 ] }, "GridColumnIndex": 0 }, { "Label": "Pos9", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.82], "Focus": [17741.3], "XYStage": [ 19731.9, 31506.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos10", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.79], "Focus": [17741.4], "XYStage": [ 14528.5, 32007.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos11", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.83], "Focus": [17741.2], "XYStage": [ 13830.300000000001, 32009.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos12", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.79], "Focus": [17741.3], "XYStage": [ 8775.300000000001, 32017.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos13", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.815], "Focus": [17741.3], "XYStage": [ 8109.200000000001, 32008.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos14", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.805], "Focus": [17741.3], "XYStage": [ 3483.3, 31908.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos15", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.82], "Focus": [17741.2], "XYStage": [ 2686.8, 31903.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos16", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [211.48000000000002], "Focus": [17741.1], "XYStage": [ -1978.9, 31973.600000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos17", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [211.51500000000001], "Focus": [17741.1], "XYStage": [ -2957.8, 31982.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos18", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.195], "Focus": [17741.5], "XYStage": [ 47747.4, -576.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos19", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.23000000000002], "Focus": [17741.6], "XYStage": [ 47053.9, -576.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos20", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.205], "Focus": [17741.4], "XYStage": [ 41871.200000000004, -479.8 ] }, "GridColumnIndex": 0 }, { "Label": "Pos21", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.4], "XYStage": [ 40882.600000000006, -392 ] }, "GridColumnIndex": 0 }, { "Label": "Pos22", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.24], "Focus": [17741.4], "XYStage": [ 35905.6, -135.3 ] }, "GridColumnIndex": 0 }, { "Label": "Pos23", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.195], "Focus": [17741.5], "XYStage": [ 34990.9, -132.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos24", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.46], "Focus": [17741.4], "XYStage": [ 29678.100000000002, -958.1 ] }, "GridColumnIndex": 0 }, { "Label": "Pos25", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.44], "Focus": [17741.5], "XYStage": [ 28983.600000000002, -939.4000000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos26", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.475], "Focus": [17741.5], "XYStage": [ 23019.7, -122 ] }, "GridColumnIndex": 0 }, { "Label": "Pos27", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.44], "Focus": [17741.7], "XYStage": [ 22428, -116.80000000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos28", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.46], "Focus": [17741.7], "XYStage": [ 16364.900000000001, -181.10000000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos29", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.48000000000002], "Focus": [17741.5], "XYStage": [ 15712.1, -180 ] }, "GridColumnIndex": 0 }, { "Label": "Pos30", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.45000000000002], "Focus": [17741.7], "XYStage": [ 10346.1, 77.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos31", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.49], "Focus": [17741.4], "XYStage": [ 9755.5, 85.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos32", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.475], "Focus": [17741.5], "XYStage": [ 4775.5, 293.90000000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos33", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.445], "Focus": [17741.4], "XYStage": [ 3954.9, 293.90000000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos34", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.455], "Focus": [17741.4], "XYStage": [ -1324.9, 545.6 ] }, "GridColumnIndex": 0 }, { "Label": "Pos35", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.445], "Focus": [17741.3], "XYStage": [ -2097, 576.6 ] }, "GridColumnIndex": 0 } ], "z-step_um": 10, "GridRow": 0, "BitDepth": 16, "Depth": 2, "Channels": 6, "Source": "Micro-Manager", "ChColors": [ -16711681, -256, -65281, -1, -1, -16776961 ], "MetadataVersion": 10, "KeepShutterOpenChannels": true, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536, 65536 ], "CustomIntervals_ms": [], "Height": 1200, "MicroManagerVersion": "1.4.23 20180219", "GridColumn": 0, "UUID": "18808e7c-f2e6-47b3-90d8-1d779ce87dbd", "PixelType": "GRAY16", "Width": 1200, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\Yan\\20191218-YC113-S7S8-miseq", "Frames": 1, "Comment": "", "UserName": "Nikon", "PixelSize_um": 1.1, "ROI": [ 0, 0, 1200, 1200 ], "Date": "2019-12-19", "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC", "P-DAPI-high" ], "KeepShutterOpenSlices": true, "IJType": 1, "ComputerName": "ZadorConfocal2", "Positions": 36, "PositionIndex": 34, "CameraTimeout": 20000, "TimeFirst": false }, "640 AO-Name": "AnalogIO", "Vincent-D3-Command": "Close" }, "FrameKey-0-0-3": { "COM4-StopBits": "1", "528 AO-MaxVolts": "5.0000", "XLIGHT Excitation Wheel-State": "1", "470 Shutter-Name": "AnalogIO", "Core-Shutter": "520", "555 AO-Demo": "No", "XLIGHT Dichroic-State": "2", "XYStage-StepSize-Y": "0.1000", "ZPositionUm": 198.455, "XYStage-StepSize-X": "0.1000", "PRIME-FirmwareVersion": "20.28", "PRIME-ClearCycles": "2", "520-Name": "DA Shutter", "405 Shutter-Name": "AnalogIO", "PIEZO-StepSize": "0.0050", "PRIME-ClearMode": "Pre-Exposure", "470 AO-MinVolts": "0.0000", "405 AO-SupportsTriggering": "1", "CameraChannelIndex": 0, "XYStage-StartSpeed": "500.0000", "COM9-StopBits": "2", "405 AO-Description": "NI DAC", "COM4-DelayBetweenCharsMs": "0.0000", "445 AO-Block voltage": "false", "520 AO-TriggerInputLine": "/Dev1/PFI0", "555 AO-Description": "NI DAC", "520 AO-TriggerSampleRate": "100000", "COM4-Verbose": "1", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM4-Name": "COM4", "405 Shutter-Volts": "0.0000", "Vincent-D3-Port": "COM1", "PRIME-PP 4 MIN ADU AFFECTED": "200", "470 Shutter-MinVolts": "0.0000", "PRIME-PP 3 ENABLED": "Yes", "470 AO-Description": "NI DAC", "PRIME-Description": "PVCAM API device adapter", "520 Shutter-MaxVolts": "5.0000", "520 Shutter-Volts": "5.0000", "528 AO-Sequenceable": "1", "PRIME-Binning": "1x1", "555 Shutter-MaxVolts": "5.0000", "528 Shutter-TriggerSequenceLength": "1000", "405 Shutter-Block voltage": "false", "XLIGHT Spin motor-State": "1", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "Vincent-D3-Time to open (ms)": "35.0000", "Vincent-D3-Time to close (ms)": "35.0000", "445 AO-TriggerSampleRate": "100000", "640 Shutter-IOChannel": "Dev1/ao14", "555 AO-Sequenceable": "1", "520 AO-Name": "AnalogIO", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "470 AO-Block voltage": "false", "COM1-Verbose": "1", "COM9-AnswerTimeout": "10000.0000", "528 Shutter-Name": "AnalogIO", "PRIME-Trigger Timeout (secs)": "10", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "NextFrame": 0, "470 AO-TriggerSequenceLength": "1000", "PRIME-TransposeXY": "0", "445 AO-TriggerSequenceLength": "1000", "COM1-Name": "COM1", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "COM9-DataBits": "8", "COM1-AnswerTimeout": "500.0000", "Camera": "", "TE2000-Version": "V2.50-V2.50-V2.10", "528 Shutter-Volts": "0.0000", "XLIGHT Hub-Name": "XLIGHT Hub", "PIEZO-Description": "Ludl stage driver adapter", "PositionName": "Pos34", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSampleRate": "100000", "PRIME-FanSpeedSetpoint": "High", "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "405 AO-TriggerSampleRate": "100000", "470 Shutter-Block voltage": "false", "470-State": "0", "COM3-Parity": "None", "405 AO-Volts": "0.0000", "PRIME-TransposeMirrorY": "0", "PRIME-ChipName": "GS144BSI", "470 AO-Volts": "0.0000", "PRIME-TransposeMirrorX": "0", "445 Shutter-SupportsTriggering": "0", "470 Shutter-SupportsTriggering": "0", "XLIGHT Hub-Description": "XLIGHT hub", "PRIME-PreampOffLimit": "10000", "470 AO-Sequenceable": "1", "528 Shutter-MaxVolts": "5.0000", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "Core-XYStage": "XYStage", "470 AO-TriggerSampleRate": "100000", "PRIME-Timing-PreTriggerDelayNs": "24672000.0000", "528-DA Device": "528 Shutter", "Focus-Description": "Nikon TE2000 focus adapter", "FilterBlock-State": "5", "520 AO-Demo": "No", "COM3-DelayBetweenCharsMs": "0.0000", "470 Shutter-Volts": "0.0000", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "PRIME-PP 2 THRESHOLD": "125", "PRIME-PP 4 THRESHOLD": "75", "XYStage-Acceleration": "4.0000", "470 Shutter-Demo": "No", "PRIME-Name": "PMPCIECam00", "640-DA Device": "640 Shutter", "405 AO-Block voltage": "false", "ElapsedTime-ms": 1392797, "PRIME-Offset": "100", "445 AO-IOChannel": "Dev1/ao1", "640 AO-MaxVolts": "5.0000", "PositionIndex": 34, "PRIME-PP 1 ENABLED": "Yes", "COM1-BaudRate": "9600", "640-State": "0", "COM3-Verbose": "1", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "LudlController-TransmissionDelay": "0", "PRIME-FullWellCapacity": "62000", "PRIME-SMARTStreamingEnabled": "No", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "640 Shutter-Description": "NI DAC", "528-Description": "DA device that is used as a shutter", "COM4-Description": "Serial port driver (boost:asio)", "405 Shutter-Description": "NI DAC", "TE2000-Name": "TE2000", "528 Shutter-Description": "NI DAC", "COM9-Description": "Serial port driver (boost:asio)", "LudlController-Name": "LudlController", "445 Shutter-TriggerSequenceLength": "1000", "PRIME-SerialNumber": "A18B203008", "445 AO-Volts": "0.0000", "PRIME-X-dimension": "1200", "PRIME-ShutterCloseDelay": "0", "XLIGHT Disk slider-State": "2", "Position": "Default", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 AO-IOChannel": "Dev1/ao5", "520 AO-IOChannel": "Dev1/ao4", "528 AO-TriggerSequenceLength": "1000", "405 Shutter-MaxVolts": "5.0000", "470-DA Device": "470 Shutter", "PRIME-FTCapable": "No", "Core-ChannelGroup": "Channels", "555 AO-SupportsTriggering": "0", "PRIME-Y-dimension": "1200", "Frame": 0, "555 Shutter-Sequenceable": "1", "PRIME-Timing-ExposureTimeNs": "499998000.0000", "Core-ImageProcessor": "", "528-State": "0", "520 AO-Block voltage": "false", "XLIGHT Excitation Wheel-Label": "CFP/YFP", "640 Shutter-Demo": "No", "COM3-StopBits": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "520 Shutter-IOChannel": "Dev1/ao11", "Width": 1200, "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-SupportsTriggering": "0", "640 AO-TriggerSampleRate": "100000", "520-Description": "DA device that is used as a shutter", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "405 Shutter-Sequenceable": "1", "405 Shutter-SupportsTriggering": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-TriggerSampleRate": "100000", "640 Shutter-MaxVolts": "5.0000", "555 AO-MaxVolts": "5.0000", "520 AO-TriggerSequenceLength": "1000", "COM3-BaudRate": "9600", "COM9-BaudRate": "9600", "Vincent-D3-Channel #": "Ch. #1", "PRIME-PMode": "Normal", "OpticalPath-Label": "Left(100)", "BitDepth": 16, "555 AO-TriggerSequenceLength": "1000", "445 Shutter-Description": "NI DAC", "405 AO-Sequenceable": "1", "PRIME-AcquisitionMethod": "Callbacks", "555 AO-Name": "AnalogIO", "PRIME-ShutterMode": "Pre-Exposure", "COM3-Handshaking": "Off", "445 AO-SupportsTriggering": "0", "ROI": "0-0-1200-1200", "COM9-Verbose": "1", "PRIME-Color": "OFF", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "PRIME-ShutterOpenDelay": "0", "COM1-Parity": "None", "Slice": 3, "SlicePosition": -20, "COM9-DelayBetweenCharsMs": "0.0000", "555-Name": "DA Shutter", "PIEZO-LudlSingleAxisName": "B", "XLIGHT Emission Wheel-Label": "565/24(G)", "640 Shutter-Name": "AnalogIO", "640 AO-Sequenceable": "1", "520 Shutter-Demo": "No", "528 Shutter-Demo": "No", "470-Name": "DA Shutter", "520-DA Device": "520 Shutter", "XYStage-Description": "Ludl XY stage driver adapter", "445 Shutter-MinVolts": "0.0000", "528 AO-TriggerSampleRate": "100000", "405 AO-MinVolts": "0.0000", "405 Shutter-TriggerSequenceLength": "1000", "Height": 1200, "555 AO-Block voltage": "false", "640 Shutter-Volts": "0.0000", "PixelType": "GRAY16", "445 Shutter-Block voltage": "false", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "520 Shutter-TriggerSequenceLength": "1000", "COM9-Name": "COM9", "Core-SLM": "", "555 AO-Volts": "0.0000", "555 Shutter-Block voltage": "false", "XYStage-Speed": "1800.0000", "520 AO-MaxVolts": "5.0000", "528 Shutter-SupportsTriggering": "0", "COM1-Description": "Serial port driver (boost:asio)", "PRIME-MetadataEnabled": "No", "520 Shutter-MinVolts": "0.0000", "470 Shutter-Sequenceable": "1", "PRIME-PreampDelay": "0", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-MinVolts": "0.0000", "ChannelIndex": 0, "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Core-AutoShutter": "1", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "Exposure-ms": 320, "PRIME-PP 2 ENABLED": "Yes", "640 Shutter-TriggerSampleRate": "100000", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "470 Shutter-IOChannel": "Dev1/ao10", "405 AO-Demo": "No", "PixelSizeUm": 1.1, "470 AO-Demo": "No", "470 Shutter-MaxVolts": "5.0000", "Vincent-D3-Name": "Vincent-D3", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FileName": "img_000000000_Il-G_003.tif", "LudlController-Reset": "Operate", "PRIME-Exposure": "320.00", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "445-Name": "DA Shutter", "555 Shutter-Name": "AnalogIO", "520 Shutter-TriggerSampleRate": "100000", "445 Shutter-IOChannel": "Dev1/ao9", "PRIME-TransposeCorrection": "0", "XYStage-StepSize": "0.1000", "445-Description": "DA device that is used as a shutter", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 AO-IOChannel": "Dev1/ao7", "520 Shutter-Name": "AnalogIO", "COM3-DataBits": "8", "640 AO-Volts": "0.0000", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "555 Shutter-IOChannel": "Dev1/ao13", "WaitInterval": null, "520 Shutter-Sequenceable": "1", "YPositionUm": 545.6, "445-State": "0", "445-DA Device": "445 Shutter", "PRIME-CircularBufferAutoSize": "ON", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "555 AO-TriggerSampleRate": "100000", "445 Shutter-Volts": "0.0000", "XLIGHT Hub-Port": "COM3", "Core-AutoFocus": "", "COM4-Handshaking": "Off", "528 Shutter-MinVolts": "0.0000", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "445 Shutter-Name": "AnalogIO", "528 Shutter-Sequenceable": "1", "XYStage-TransposeMirrorX": "0", "XYStage-TransposeMirrorY": "0", "PRIME-ReadoutRate": "100MHz 16bit", "XYStage-Name": "Stage", "640 AO-SupportsTriggering": "0", "640-Name": "DA Shutter", "640 Shutter-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "640 AO-Block voltage": "false", "445 Shutter-Sequenceable": "1", "470 AO-MaxVolts": "5.0000", "COM4-BaudRate": "9600", "470-Description": "DA device that is used as a shutter", "520 Shutter-Description": "NI DAC", "PRIME-CCDTemperature": "-14.8500", "Time": "2019-12-19 09:59:23 -0500", "640 AO-TriggerSequenceLength": "1000", "470 AO-Name": "AnalogIO", "XLIGHT Disk slider-Label": "Disk Pos 40um", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "Binning": "1x1", "TE2000-ModelType": "1", "COM3-AnswerTimeout": "500.0000", "555 Shutter-SupportsTriggering": "0", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "TE2000-Port": "COM4", "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Description": "NI DAC", "COM4-AnswerTimeout": "500.0000", "COM1-DelayBetweenCharsMs": "0.0000", "FilterBlock-Label": "Confocal", "445 AO-Name": "AnalogIO", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-Timing-ReadoutTimeNs": "24672000.0000", "528 AO-Demo": "No", "520 AO-Sequenceable": "1", "555-Description": "DA device that is used as a shutter", "405-DA Device": "405 Shutter", "405 AO-TriggerSequenceLength": "1000", "640 AO-MinVolts": "0.0000", "640 Shutter-Block voltage": "false", "FrameIndex": 0, "PRIME-Port": "CMOS", "555 AO-MinVolts": "0.0000", "COM3-Description": "Serial port driver (boost:asio)", "528 AO-Description": "NI DAC", "555 AO-IOChannel": "Dev1/ao6", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "555 AO-TriggerInputLine": "/Dev1/PFI0", "PRIME-ActualInterval-ms": "390.5200", "LudlController-Port": "COM9", "445 Shutter-Demo": "No", "PRIME-Trigger-Expose Out-Mux": "1", "SliceIndex": 3, "PRIME-CCDTemperatureSetPoint": "-15.0000", "405 Shutter-MinVolts": "0.0000", "PRIME-ExposeOutMode": "First Row", "AxisPositions": null, "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Volts": "0.0000", "528-Name": "DA Shutter", "555-State": "0", "XLIGHT Dichroic-Label": "CFP/YFP", "445 AO-Sequenceable": "1", "405-Name": "DA Shutter", "405 AO-IOChannel": "Dev1/ao0", "Core-Camera": "PRIME", "Focus-Name": "Focus", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "405 Shutter-IOChannel": "Dev1/ao8", "640 AO-Demo": "No", "640 Shutter-SupportsTriggering": "0", "445 AO-Demo": "No", "555-DA Device": "555 Shutter", "470 Shutter-Description": "NI DAC", "528 AO-Volts": "0.0000", "COM1-DataBits": "8", "Vincent-D3-Address": "x", "520 AO-SupportsTriggering": "0", "405-Description": "DA device that is used as a shutter", "640 Shutter-MinVolts": "0.0000", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "405 AO-Name": "AnalogIO", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "640 AO-TriggerInputLine": "/Dev1/PFI0", "405 AO-TriggerInputLine": "/Dev1/PFI0", "640-Description": "DA device that is used as a shutter", "445 Shutter-TriggerSampleRate": "100000", "470 Shutter-TriggerSequenceLength": "1000", "COM1-StopBits": "1", "520 Shutter-SupportsTriggering": "0", "405-State": "0", "Core-Initialize": "1", "XLIGHT Emission Wheel-State": "3", "520 AO-Description": "NI DAC", "528 AO-TriggerInputLine": "/Dev1/PFI0", "445 Shutter-MaxVolts": "5.0000", "COM9-Handshaking": "Off", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "XLIGHT Spin motor-Label": "On (spinning)", "PRIME-PP 5 ENABLED": "No", "UUID": "811f496d-9eab-4739-812d-340de0f1773b", "PRIME-TriggerMode": "Internal Trigger", "COM1-Handshaking": "Off", "PRIME-Timing-PostTriggerDelayNs": "49344000.0000", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "Focus-Position": "17741.5000", "528 Shutter-Block voltage": "false", "Channel": "Il-G", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "555 Shutter-Demo": "No", "405 Shutter-Demo": "No", "528 AO-Name": "AnalogIO", "520-State": "1", "Core-Galvo": "", "405 Shutter-TriggerSampleRate": "100000", "Focus-FocusStepSize_(nm)": "100", "PRIME-PixelType": "16bit", "COM4-DataBits": "8", "470 AO-IOChannel": "Dev1/ao2", "520 AO-MinVolts": "0.0000", "COM9-Parity": "None", "PRIME-CircularBufferEnabled": "ON", "PRIME-PP 6 Reset": "No", "640 AO-Description": "NI DAC", "Source": "PRIME", "445 AO-MinVolts": "0.0000", "PIEZO-Name": "Stage", "445 AO-Description": "NI DAC", "PRIME-CircularBufferFrameCount": "46", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "520 AO-Volts": "4.5000", "528 Shutter-TriggerSampleRate": "100000", "XPositionUm": -1324.9, "OpticalPath-State": "4", "PRIME-PP 3 THRESHOLD": "80", "PRIME-PVCAM Version": "3.7.1", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Gain": "1-HDR", "640 Shutter-TriggerSequenceLength": "1000", "528 AO-SupportsTriggering": "0", "445 AO-TriggerInputLine": "/Dev1/PFI0", "OpticalPath-Name": "OpticalPath", "PRIME-PP 4 ENABLED": "Yes", "PRIME-PVCAM Adapter Version": "1.3.29", "COM3-Name": "COM3", "405 AO-MaxVolts": "5.0000", "520 Shutter-Block voltage": "false", "PRIME-Timing-ClearingTimeNs": "49344000.0000", "Core-Focus": "PIEZO", "528 AO-Block voltage": "false", "TE2000-Description": "Nikon TE2000 hub", "528 AO-MinVolts": "0.0000", "COM4-Parity": "None", "Summary": { "Slices": 11, "ChContrastMin": [ 0, 0, 0, 0, 0, 0 ], "PixelAspect": 1, "SlicesFirst": true, "Interval_ms": 0, "Time": "2019-12-19 09:59:21 -0500", "Prefix": "seq_13", "InitialPositionList": [ { "Label": "Pos0", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.21], "Focus": [17741.5], "XYStage": [ 44542.200000000004, 31636.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos1", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.22], "Focus": [17741.4], "XYStage": [ 43801.100000000006, 31637.9 ] }, "GridColumnIndex": 0 }, { "Label": "Pos2", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.5], "XYStage": [ 38561.3, 32040.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos3", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.185], "Focus": [17741.3], "XYStage": [ 37698.4, 32041.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos4", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.16], "Focus": [17741.5], "XYStage": [ 32375.4, 31758.9 ] }, "GridColumnIndex": 0 }, { "Label": "Pos5", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.17000000000002], "Focus": [17741.3], "XYStage": [ 31770.7, 31758.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos6", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.4], "XYStage": [ 26536.800000000003, 31068.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos7", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.205], "Focus": [17741.4], "XYStage": [ 25420.2, 31068.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos8", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.85], "Focus": [17741.3], "XYStage": [ 21059, 31508 ] }, "GridColumnIndex": 0 }, { "Label": "Pos9", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.82], "Focus": [17741.3], "XYStage": [ 19731.9, 31506.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos10", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.79], "Focus": [17741.4], "XYStage": [ 14528.5, 32007.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos11", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.83], "Focus": [17741.2], "XYStage": [ 13830.300000000001, 32009.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos12", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.79], "Focus": [17741.3], "XYStage": [ 8775.300000000001, 32017.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos13", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.815], "Focus": [17741.3], "XYStage": [ 8109.200000000001, 32008.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos14", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.805], "Focus": [17741.3], "XYStage": [ 3483.3, 31908.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos15", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.82], "Focus": [17741.2], "XYStage": [ 2686.8, 31903.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos16", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [211.48000000000002], "Focus": [17741.1], "XYStage": [ -1978.9, 31973.600000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos17", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [211.51500000000001], "Focus": [17741.1], "XYStage": [ -2957.8, 31982.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos18", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.195], "Focus": [17741.5], "XYStage": [ 47747.4, -576.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos19", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.23000000000002], "Focus": [17741.6], "XYStage": [ 47053.9, -576.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos20", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.205], "Focus": [17741.4], "XYStage": [ 41871.200000000004, -479.8 ] }, "GridColumnIndex": 0 }, { "Label": "Pos21", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.4], "XYStage": [ 40882.600000000006, -392 ] }, "GridColumnIndex": 0 }, { "Label": "Pos22", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.24], "Focus": [17741.4], "XYStage": [ 35905.6, -135.3 ] }, "GridColumnIndex": 0 }, { "Label": "Pos23", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.195], "Focus": [17741.5], "XYStage": [ 34990.9, -132.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos24", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.46], "Focus": [17741.4], "XYStage": [ 29678.100000000002, -958.1 ] }, "GridColumnIndex": 0 }, { "Label": "Pos25", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.44], "Focus": [17741.5], "XYStage": [ 28983.600000000002, -939.4000000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos26", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.475], "Focus": [17741.5], "XYStage": [ 23019.7, -122 ] }, "GridColumnIndex": 0 }, { "Label": "Pos27", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.44], "Focus": [17741.7], "XYStage": [ 22428, -116.80000000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos28", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.46], "Focus": [17741.7], "XYStage": [ 16364.900000000001, -181.10000000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos29", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.48000000000002], "Focus": [17741.5], "XYStage": [ 15712.1, -180 ] }, "GridColumnIndex": 0 }, { "Label": "Pos30", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.45000000000002], "Focus": [17741.7], "XYStage": [ 10346.1, 77.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos31", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.49], "Focus": [17741.4], "XYStage": [ 9755.5, 85.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos32", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.475], "Focus": [17741.5], "XYStage": [ 4775.5, 293.90000000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos33", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.445], "Focus": [17741.4], "XYStage": [ 3954.9, 293.90000000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos34", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.455], "Focus": [17741.4], "XYStage": [ -1324.9, 545.6 ] }, "GridColumnIndex": 0 }, { "Label": "Pos35", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.445], "Focus": [17741.3], "XYStage": [ -2097, 576.6 ] }, "GridColumnIndex": 0 } ], "z-step_um": 10, "GridRow": 0, "BitDepth": 16, "Depth": 2, "Channels": 6, "Source": "Micro-Manager", "ChColors": [ -16711681, -256, -65281, -1, -1, -16776961 ], "MetadataVersion": 10, "KeepShutterOpenChannels": true, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536, 65536 ], "CustomIntervals_ms": [], "Height": 1200, "MicroManagerVersion": "1.4.23 20180219", "GridColumn": 0, "UUID": "18808e7c-f2e6-47b3-90d8-1d779ce87dbd", "PixelType": "GRAY16", "Width": 1200, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\Yan\\20191218-YC113-S7S8-miseq", "Frames": 1, "Comment": "", "UserName": "Nikon", "PixelSize_um": 1.1, "ROI": [ 0, 0, 1200, 1200 ], "Date": "2019-12-19", "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC", "P-DAPI-high" ], "KeepShutterOpenSlices": true, "IJType": 1, "ComputerName": "ZadorConfocal2", "Positions": 36, "PositionIndex": 34, "CameraTimeout": 20000, "TimeFirst": false }, "640 AO-Name": "AnalogIO", "Vincent-D3-Command": "Close" }, "FrameKey-0-0-4": { "COM4-StopBits": "1", "528 AO-MaxVolts": "5.0000", "XLIGHT Excitation Wheel-State": "1", "470 Shutter-Name": "AnalogIO", "Core-Shutter": "520", "555 AO-Demo": "No", "XLIGHT Dichroic-State": "2", "XYStage-StepSize-Y": "0.1000", "ZPositionUm": 208.455, "XYStage-StepSize-X": "0.1000", "PRIME-FirmwareVersion": "20.28", "PRIME-ClearCycles": "2", "520-Name": "DA Shutter", "405 Shutter-Name": "AnalogIO", "PIEZO-StepSize": "0.0050", "PRIME-ClearMode": "Pre-Exposure", "470 AO-MinVolts": "0.0000", "405 AO-SupportsTriggering": "1", "CameraChannelIndex": 0, "XYStage-StartSpeed": "500.0000", "COM9-StopBits": "2", "405 AO-Description": "NI DAC", "COM4-DelayBetweenCharsMs": "0.0000", "445 AO-Block voltage": "false", "520 AO-TriggerInputLine": "/Dev1/PFI0", "555 AO-Description": "NI DAC", "520 AO-TriggerSampleRate": "100000", "COM4-Verbose": "1", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM4-Name": "COM4", "405 Shutter-Volts": "0.0000", "Vincent-D3-Port": "COM1", "PRIME-PP 4 MIN ADU AFFECTED": "200", "470 Shutter-MinVolts": "0.0000", "PRIME-PP 3 ENABLED": "Yes", "470 AO-Description": "NI DAC", "PRIME-Description": "PVCAM API device adapter", "520 Shutter-MaxVolts": "5.0000", "520 Shutter-Volts": "5.0000", "528 AO-Sequenceable": "1", "PRIME-Binning": "1x1", "555 Shutter-MaxVolts": "5.0000", "528 Shutter-TriggerSequenceLength": "1000", "405 Shutter-Block voltage": "false", "XLIGHT Spin motor-State": "1", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "Vincent-D3-Time to open (ms)": "35.0000", "Vincent-D3-Time to close (ms)": "35.0000", "445 AO-TriggerSampleRate": "100000", "640 Shutter-IOChannel": "Dev1/ao14", "555 AO-Sequenceable": "1", "520 AO-Name": "AnalogIO", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "470 AO-Block voltage": "false", "COM1-Verbose": "1", "COM9-AnswerTimeout": "10000.0000", "528 Shutter-Name": "AnalogIO", "PRIME-Trigger Timeout (secs)": "10", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "NextFrame": 0, "470 AO-TriggerSequenceLength": "1000", "PRIME-TransposeXY": "0", "445 AO-TriggerSequenceLength": "1000", "COM1-Name": "COM1", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "COM9-DataBits": "8", "COM1-AnswerTimeout": "500.0000", "Camera": "", "TE2000-Version": "V2.50-V2.50-V2.10", "528 Shutter-Volts": "0.0000", "XLIGHT Hub-Name": "XLIGHT Hub", "PIEZO-Description": "Ludl stage driver adapter", "PositionName": "Pos34", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSampleRate": "100000", "PRIME-FanSpeedSetpoint": "High", "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "405 AO-TriggerSampleRate": "100000", "470 Shutter-Block voltage": "false", "470-State": "0", "COM3-Parity": "None", "405 AO-Volts": "0.0000", "PRIME-TransposeMirrorY": "0", "PRIME-ChipName": "GS144BSI", "470 AO-Volts": "0.0000", "PRIME-TransposeMirrorX": "0", "445 Shutter-SupportsTriggering": "0", "470 Shutter-SupportsTriggering": "0", "XLIGHT Hub-Description": "XLIGHT hub", "PRIME-PreampOffLimit": "10000", "470 AO-Sequenceable": "1", "528 Shutter-MaxVolts": "5.0000", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "Core-XYStage": "XYStage", "470 AO-TriggerSampleRate": "100000", "PRIME-Timing-PreTriggerDelayNs": "24672000.0000", "528-DA Device": "528 Shutter", "Focus-Description": "Nikon TE2000 focus adapter", "FilterBlock-State": "5", "520 AO-Demo": "No", "COM3-DelayBetweenCharsMs": "0.0000", "470 Shutter-Volts": "0.0000", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "PRIME-PP 2 THRESHOLD": "125", "PRIME-PP 4 THRESHOLD": "75", "XYStage-Acceleration": "4.0000", "470 Shutter-Demo": "No", "PRIME-Name": "PMPCIECam00", "640-DA Device": "640 Shutter", "405 AO-Block voltage": "false", "ElapsedTime-ms": 1393362, "PRIME-Offset": "100", "445 AO-IOChannel": "Dev1/ao1", "640 AO-MaxVolts": "5.0000", "PositionIndex": 34, "PRIME-PP 1 ENABLED": "Yes", "COM1-BaudRate": "9600", "640-State": "0", "COM3-Verbose": "1", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "LudlController-TransmissionDelay": "0", "PRIME-FullWellCapacity": "62000", "PRIME-SMARTStreamingEnabled": "No", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "640 Shutter-Description": "NI DAC", "528-Description": "DA device that is used as a shutter", "COM4-Description": "Serial port driver (boost:asio)", "405 Shutter-Description": "NI DAC", "TE2000-Name": "TE2000", "528 Shutter-Description": "NI DAC", "COM9-Description": "Serial port driver (boost:asio)", "LudlController-Name": "LudlController", "445 Shutter-TriggerSequenceLength": "1000", "PRIME-SerialNumber": "A18B203008", "445 AO-Volts": "0.0000", "PRIME-X-dimension": "1200", "PRIME-ShutterCloseDelay": "0", "XLIGHT Disk slider-State": "2", "Position": "Default", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 AO-IOChannel": "Dev1/ao5", "520 AO-IOChannel": "Dev1/ao4", "528 AO-TriggerSequenceLength": "1000", "405 Shutter-MaxVolts": "5.0000", "470-DA Device": "470 Shutter", "PRIME-FTCapable": "No", "Core-ChannelGroup": "Channels", "555 AO-SupportsTriggering": "0", "PRIME-Y-dimension": "1200", "Frame": 0, "555 Shutter-Sequenceable": "1", "PRIME-Timing-ExposureTimeNs": "499998000.0000", "Core-ImageProcessor": "", "528-State": "0", "520 AO-Block voltage": "false", "XLIGHT Excitation Wheel-Label": "CFP/YFP", "640 Shutter-Demo": "No", "COM3-StopBits": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "520 Shutter-IOChannel": "Dev1/ao11", "Width": 1200, "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-SupportsTriggering": "0", "640 AO-TriggerSampleRate": "100000", "520-Description": "DA device that is used as a shutter", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "405 Shutter-Sequenceable": "1", "405 Shutter-SupportsTriggering": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-TriggerSampleRate": "100000", "640 Shutter-MaxVolts": "5.0000", "555 AO-MaxVolts": "5.0000", "520 AO-TriggerSequenceLength": "1000", "COM3-BaudRate": "9600", "COM9-BaudRate": "9600", "Vincent-D3-Channel #": "Ch. #1", "PRIME-PMode": "Normal", "OpticalPath-Label": "Left(100)", "BitDepth": 16, "555 AO-TriggerSequenceLength": "1000", "445 Shutter-Description": "NI DAC", "405 AO-Sequenceable": "1", "PRIME-AcquisitionMethod": "Callbacks", "555 AO-Name": "AnalogIO", "PRIME-ShutterMode": "Pre-Exposure", "COM3-Handshaking": "Off", "445 AO-SupportsTriggering": "0", "ROI": "0-0-1200-1200", "COM9-Verbose": "1", "PRIME-Color": "OFF", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "PRIME-ShutterOpenDelay": "0", "COM1-Parity": "None", "Slice": 4, "SlicePosition": -10, "COM9-DelayBetweenCharsMs": "0.0000", "555-Name": "DA Shutter", "PIEZO-LudlSingleAxisName": "B", "XLIGHT Emission Wheel-Label": "565/24(G)", "640 Shutter-Name": "AnalogIO", "640 AO-Sequenceable": "1", "520 Shutter-Demo": "No", "528 Shutter-Demo": "No", "470-Name": "DA Shutter", "520-DA Device": "520 Shutter", "XYStage-Description": "Ludl XY stage driver adapter", "445 Shutter-MinVolts": "0.0000", "528 AO-TriggerSampleRate": "100000", "405 AO-MinVolts": "0.0000", "405 Shutter-TriggerSequenceLength": "1000", "Height": 1200, "555 AO-Block voltage": "false", "640 Shutter-Volts": "0.0000", "PixelType": "GRAY16", "445 Shutter-Block voltage": "false", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "520 Shutter-TriggerSequenceLength": "1000", "COM9-Name": "COM9", "Core-SLM": "", "555 AO-Volts": "0.0000", "555 Shutter-Block voltage": "false", "XYStage-Speed": "1800.0000", "520 AO-MaxVolts": "5.0000", "528 Shutter-SupportsTriggering": "0", "COM1-Description": "Serial port driver (boost:asio)", "PRIME-MetadataEnabled": "No", "520 Shutter-MinVolts": "0.0000", "470 Shutter-Sequenceable": "1", "PRIME-PreampDelay": "0", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-MinVolts": "0.0000", "ChannelIndex": 0, "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Core-AutoShutter": "1", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "Exposure-ms": 320, "PRIME-PP 2 ENABLED": "Yes", "640 Shutter-TriggerSampleRate": "100000", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "470 Shutter-IOChannel": "Dev1/ao10", "405 AO-Demo": "No", "PixelSizeUm": 1.1, "470 AO-Demo": "No", "470 Shutter-MaxVolts": "5.0000", "Vincent-D3-Name": "Vincent-D3", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FileName": "img_000000000_Il-G_004.tif", "LudlController-Reset": "Operate", "PRIME-Exposure": "320.00", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "445-Name": "DA Shutter", "555 Shutter-Name": "AnalogIO", "520 Shutter-TriggerSampleRate": "100000", "445 Shutter-IOChannel": "Dev1/ao9", "PRIME-TransposeCorrection": "0", "XYStage-StepSize": "0.1000", "445-Description": "DA device that is used as a shutter", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 AO-IOChannel": "Dev1/ao7", "520 Shutter-Name": "AnalogIO", "COM3-DataBits": "8", "640 AO-Volts": "0.0000", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "555 Shutter-IOChannel": "Dev1/ao13", "WaitInterval": null, "520 Shutter-Sequenceable": "1", "YPositionUm": 545.6, "445-State": "0", "445-DA Device": "445 Shutter", "PRIME-CircularBufferAutoSize": "ON", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "555 AO-TriggerSampleRate": "100000", "445 Shutter-Volts": "0.0000", "XLIGHT Hub-Port": "COM3", "Core-AutoFocus": "", "COM4-Handshaking": "Off", "528 Shutter-MinVolts": "0.0000", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "445 Shutter-Name": "AnalogIO", "528 Shutter-Sequenceable": "1", "XYStage-TransposeMirrorX": "0", "XYStage-TransposeMirrorY": "0", "PRIME-ReadoutRate": "100MHz 16bit", "XYStage-Name": "Stage", "640 AO-SupportsTriggering": "0", "640-Name": "DA Shutter", "640 Shutter-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "640 AO-Block voltage": "false", "445 Shutter-Sequenceable": "1", "470 AO-MaxVolts": "5.0000", "COM4-BaudRate": "9600", "470-Description": "DA device that is used as a shutter", "520 Shutter-Description": "NI DAC", "PRIME-CCDTemperature": "-14.8500", "Time": "2019-12-19 09:59:23 -0500", "640 AO-TriggerSequenceLength": "1000", "470 AO-Name": "AnalogIO", "XLIGHT Disk slider-Label": "Disk Pos 40um", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "Binning": "1x1", "TE2000-ModelType": "1", "COM3-AnswerTimeout": "500.0000", "555 Shutter-SupportsTriggering": "0", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "TE2000-Port": "COM4", "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Description": "NI DAC", "COM4-AnswerTimeout": "500.0000", "COM1-DelayBetweenCharsMs": "0.0000", "FilterBlock-Label": "Confocal", "445 AO-Name": "AnalogIO", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-Timing-ReadoutTimeNs": "24672000.0000", "528 AO-Demo": "No", "520 AO-Sequenceable": "1", "555-Description": "DA device that is used as a shutter", "405-DA Device": "405 Shutter", "405 AO-TriggerSequenceLength": "1000", "640 AO-MinVolts": "0.0000", "640 Shutter-Block voltage": "false", "FrameIndex": 0, "PRIME-Port": "CMOS", "555 AO-MinVolts": "0.0000", "COM3-Description": "Serial port driver (boost:asio)", "528 AO-Description": "NI DAC", "555 AO-IOChannel": "Dev1/ao6", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "555 AO-TriggerInputLine": "/Dev1/PFI0", "PRIME-ActualInterval-ms": "390.5200", "LudlController-Port": "COM9", "445 Shutter-Demo": "No", "PRIME-Trigger-Expose Out-Mux": "1", "SliceIndex": 4, "PRIME-CCDTemperatureSetPoint": "-15.0000", "405 Shutter-MinVolts": "0.0000", "PRIME-ExposeOutMode": "First Row", "AxisPositions": null, "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Volts": "0.0000", "528-Name": "DA Shutter", "555-State": "0", "XLIGHT Dichroic-Label": "CFP/YFP", "445 AO-Sequenceable": "1", "405-Name": "DA Shutter", "405 AO-IOChannel": "Dev1/ao0", "Core-Camera": "PRIME", "Focus-Name": "Focus", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "405 Shutter-IOChannel": "Dev1/ao8", "640 AO-Demo": "No", "640 Shutter-SupportsTriggering": "0", "445 AO-Demo": "No", "555-DA Device": "555 Shutter", "470 Shutter-Description": "NI DAC", "528 AO-Volts": "0.0000", "COM1-DataBits": "8", "Vincent-D3-Address": "x", "520 AO-SupportsTriggering": "0", "405-Description": "DA device that is used as a shutter", "640 Shutter-MinVolts": "0.0000", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "405 AO-Name": "AnalogIO", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "640 AO-TriggerInputLine": "/Dev1/PFI0", "405 AO-TriggerInputLine": "/Dev1/PFI0", "640-Description": "DA device that is used as a shutter", "445 Shutter-TriggerSampleRate": "100000", "470 Shutter-TriggerSequenceLength": "1000", "COM1-StopBits": "1", "520 Shutter-SupportsTriggering": "0", "405-State": "0", "Core-Initialize": "1", "XLIGHT Emission Wheel-State": "3", "520 AO-Description": "NI DAC", "528 AO-TriggerInputLine": "/Dev1/PFI0", "445 Shutter-MaxVolts": "5.0000", "COM9-Handshaking": "Off", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "XLIGHT Spin motor-Label": "On (spinning)", "PRIME-PP 5 ENABLED": "No", "UUID": "139f6e4a-b571-4c05-9e58-4e0906cb3912", "PRIME-TriggerMode": "Internal Trigger", "COM1-Handshaking": "Off", "PRIME-Timing-PostTriggerDelayNs": "49344000.0000", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "Focus-Position": "17741.5000", "528 Shutter-Block voltage": "false", "Channel": "Il-G", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "555 Shutter-Demo": "No", "405 Shutter-Demo": "No", "528 AO-Name": "AnalogIO", "520-State": "1", "Core-Galvo": "", "405 Shutter-TriggerSampleRate": "100000", "Focus-FocusStepSize_(nm)": "100", "PRIME-PixelType": "16bit", "COM4-DataBits": "8", "470 AO-IOChannel": "Dev1/ao2", "520 AO-MinVolts": "0.0000", "COM9-Parity": "None", "PRIME-CircularBufferEnabled": "ON", "PRIME-PP 6 Reset": "No", "640 AO-Description": "NI DAC", "Source": "PRIME", "445 AO-MinVolts": "0.0000", "PIEZO-Name": "Stage", "445 AO-Description": "NI DAC", "PRIME-CircularBufferFrameCount": "46", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "520 AO-Volts": "4.5000", "528 Shutter-TriggerSampleRate": "100000", "XPositionUm": -1324.9, "OpticalPath-State": "4", "PRIME-PP 3 THRESHOLD": "80", "PRIME-PVCAM Version": "3.7.1", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Gain": "1-HDR", "640 Shutter-TriggerSequenceLength": "1000", "528 AO-SupportsTriggering": "0", "445 AO-TriggerInputLine": "/Dev1/PFI0", "OpticalPath-Name": "OpticalPath", "PRIME-PP 4 ENABLED": "Yes", "PRIME-PVCAM Adapter Version": "1.3.29", "COM3-Name": "COM3", "405 AO-MaxVolts": "5.0000", "520 Shutter-Block voltage": "false", "PRIME-Timing-ClearingTimeNs": "49344000.0000", "Core-Focus": "PIEZO", "528 AO-Block voltage": "false", "TE2000-Description": "Nikon TE2000 hub", "528 AO-MinVolts": "0.0000", "COM4-Parity": "None", "Summary": { "Slices": 11, "ChContrastMin": [ 0, 0, 0, 0, 0, 0 ], "PixelAspect": 1, "SlicesFirst": true, "Interval_ms": 0, "Time": "2019-12-19 09:59:21 -0500", "Prefix": "seq_13", "InitialPositionList": [ { "Label": "Pos0", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.21], "Focus": [17741.5], "XYStage": [ 44542.200000000004, 31636.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos1", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.22], "Focus": [17741.4], "XYStage": [ 43801.100000000006, 31637.9 ] }, "GridColumnIndex": 0 }, { "Label": "Pos2", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.5], "XYStage": [ 38561.3, 32040.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos3", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.185], "Focus": [17741.3], "XYStage": [ 37698.4, 32041.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos4", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.16], "Focus": [17741.5], "XYStage": [ 32375.4, 31758.9 ] }, "GridColumnIndex": 0 }, { "Label": "Pos5", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.17000000000002], "Focus": [17741.3], "XYStage": [ 31770.7, 31758.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos6", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.4], "XYStage": [ 26536.800000000003, 31068.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos7", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.205], "Focus": [17741.4], "XYStage": [ 25420.2, 31068.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos8", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.85], "Focus": [17741.3], "XYStage": [ 21059, 31508 ] }, "GridColumnIndex": 0 }, { "Label": "Pos9", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.82], "Focus": [17741.3], "XYStage": [ 19731.9, 31506.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos10", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.79], "Focus": [17741.4], "XYStage": [ 14528.5, 32007.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos11", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.83], "Focus": [17741.2], "XYStage": [ 13830.300000000001, 32009.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos12", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.79], "Focus": [17741.3], "XYStage": [ 8775.300000000001, 32017.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos13", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.815], "Focus": [17741.3], "XYStage": [ 8109.200000000001, 32008.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos14", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.805], "Focus": [17741.3], "XYStage": [ 3483.3, 31908.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos15", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.82], "Focus": [17741.2], "XYStage": [ 2686.8, 31903.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos16", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [211.48000000000002], "Focus": [17741.1], "XYStage": [ -1978.9, 31973.600000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos17", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [211.51500000000001], "Focus": [17741.1], "XYStage": [ -2957.8, 31982.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos18", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.195], "Focus": [17741.5], "XYStage": [ 47747.4, -576.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos19", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.23000000000002], "Focus": [17741.6], "XYStage": [ 47053.9, -576.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos20", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.205], "Focus": [17741.4], "XYStage": [ 41871.200000000004, -479.8 ] }, "GridColumnIndex": 0 }, { "Label": "Pos21", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.4], "XYStage": [ 40882.600000000006, -392 ] }, "GridColumnIndex": 0 }, { "Label": "Pos22", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.24], "Focus": [17741.4], "XYStage": [ 35905.6, -135.3 ] }, "GridColumnIndex": 0 }, { "Label": "Pos23", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.195], "Focus": [17741.5], "XYStage": [ 34990.9, -132.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos24", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.46], "Focus": [17741.4], "XYStage": [ 29678.100000000002, -958.1 ] }, "GridColumnIndex": 0 }, { "Label": "Pos25", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.44], "Focus": [17741.5], "XYStage": [ 28983.600000000002, -939.4000000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos26", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.475], "Focus": [17741.5], "XYStage": [ 23019.7, -122 ] }, "GridColumnIndex": 0 }, { "Label": "Pos27", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.44], "Focus": [17741.7], "XYStage": [ 22428, -116.80000000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos28", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.46], "Focus": [17741.7], "XYStage": [ 16364.900000000001, -181.10000000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos29", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.48000000000002], "Focus": [17741.5], "XYStage": [ 15712.1, -180 ] }, "GridColumnIndex": 0 }, { "Label": "Pos30", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.45000000000002], "Focus": [17741.7], "XYStage": [ 10346.1, 77.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos31", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.49], "Focus": [17741.4], "XYStage": [ 9755.5, 85.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos32", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.475], "Focus": [17741.5], "XYStage": [ 4775.5, 293.90000000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos33", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.445], "Focus": [17741.4], "XYStage": [ 3954.9, 293.90000000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos34", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.455], "Focus": [17741.4], "XYStage": [ -1324.9, 545.6 ] }, "GridColumnIndex": 0 }, { "Label": "Pos35", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.445], "Focus": [17741.3], "XYStage": [ -2097, 576.6 ] }, "GridColumnIndex": 0 } ], "z-step_um": 10, "GridRow": 0, "BitDepth": 16, "Depth": 2, "Channels": 6, "Source": "Micro-Manager", "ChColors": [ -16711681, -256, -65281, -1, -1, -16776961 ], "MetadataVersion": 10, "KeepShutterOpenChannels": true, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536, 65536 ], "CustomIntervals_ms": [], "Height": 1200, "MicroManagerVersion": "1.4.23 20180219", "GridColumn": 0, "UUID": "18808e7c-f2e6-47b3-90d8-1d779ce87dbd", "PixelType": "GRAY16", "Width": 1200, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\Yan\\20191218-YC113-S7S8-miseq", "Frames": 1, "Comment": "", "UserName": "Nikon", "PixelSize_um": 1.1, "ROI": [ 0, 0, 1200, 1200 ], "Date": "2019-12-19", "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC", "P-DAPI-high" ], "KeepShutterOpenSlices": true, "IJType": 1, "ComputerName": "ZadorConfocal2", "Positions": 36, "PositionIndex": 34, "CameraTimeout": 20000, "TimeFirst": false }, "640 AO-Name": "AnalogIO", "Vincent-D3-Command": "Close" }, "FrameKey-0-0-5": { "COM4-StopBits": "1", "528 AO-MaxVolts": "5.0000", "XLIGHT Excitation Wheel-State": "1", "470 Shutter-Name": "AnalogIO", "Core-Shutter": "520", "555 AO-Demo": "No", "XLIGHT Dichroic-State": "2", "XYStage-StepSize-Y": "0.1000", "ZPositionUm": 218.455, "XYStage-StepSize-X": "0.1000", "PRIME-FirmwareVersion": "20.28", "PRIME-ClearCycles": "2", "520-Name": "DA Shutter", "405 Shutter-Name": "AnalogIO", "PIEZO-StepSize": "0.0050", "PRIME-ClearMode": "Pre-Exposure", "470 AO-MinVolts": "0.0000", "405 AO-SupportsTriggering": "1", "CameraChannelIndex": 0, "XYStage-StartSpeed": "500.0000", "COM9-StopBits": "2", "405 AO-Description": "NI DAC", "COM4-DelayBetweenCharsMs": "0.0000", "445 AO-Block voltage": "false", "520 AO-TriggerInputLine": "/Dev1/PFI0", "555 AO-Description": "NI DAC", "520 AO-TriggerSampleRate": "100000", "COM4-Verbose": "1", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM4-Name": "COM4", "405 Shutter-Volts": "0.0000", "Vincent-D3-Port": "COM1", "PRIME-PP 4 MIN ADU AFFECTED": "200", "470 Shutter-MinVolts": "0.0000", "PRIME-PP 3 ENABLED": "Yes", "470 AO-Description": "NI DAC", "PRIME-Description": "PVCAM API device adapter", "520 Shutter-MaxVolts": "5.0000", "520 Shutter-Volts": "5.0000", "528 AO-Sequenceable": "1", "PRIME-Binning": "1x1", "555 Shutter-MaxVolts": "5.0000", "528 Shutter-TriggerSequenceLength": "1000", "405 Shutter-Block voltage": "false", "XLIGHT Spin motor-State": "1", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "Vincent-D3-Time to open (ms)": "35.0000", "Vincent-D3-Time to close (ms)": "35.0000", "445 AO-TriggerSampleRate": "100000", "640 Shutter-IOChannel": "Dev1/ao14", "555 AO-Sequenceable": "1", "520 AO-Name": "AnalogIO", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "470 AO-Block voltage": "false", "COM1-Verbose": "1", "COM9-AnswerTimeout": "10000.0000", "528 Shutter-Name": "AnalogIO", "PRIME-Trigger Timeout (secs)": "10", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "NextFrame": 0, "470 AO-TriggerSequenceLength": "1000", "PRIME-TransposeXY": "0", "445 AO-TriggerSequenceLength": "1000", "COM1-Name": "COM1", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "COM9-DataBits": "8", "COM1-AnswerTimeout": "500.0000", "Camera": "", "TE2000-Version": "V2.50-V2.50-V2.10", "528 Shutter-Volts": "0.0000", "XLIGHT Hub-Name": "XLIGHT Hub", "PIEZO-Description": "Ludl stage driver adapter", "PositionName": "Pos34", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSampleRate": "100000", "PRIME-FanSpeedSetpoint": "High", "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "405 AO-TriggerSampleRate": "100000", "470 Shutter-Block voltage": "false", "470-State": "0", "COM3-Parity": "None", "405 AO-Volts": "0.0000", "PRIME-TransposeMirrorY": "0", "PRIME-ChipName": "GS144BSI", "470 AO-Volts": "0.0000", "PRIME-TransposeMirrorX": "0", "445 Shutter-SupportsTriggering": "0", "470 Shutter-SupportsTriggering": "0", "XLIGHT Hub-Description": "XLIGHT hub", "PRIME-PreampOffLimit": "10000", "470 AO-Sequenceable": "1", "528 Shutter-MaxVolts": "5.0000", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "Core-XYStage": "XYStage", "470 AO-TriggerSampleRate": "100000", "PRIME-Timing-PreTriggerDelayNs": "24672000.0000", "528-DA Device": "528 Shutter", "Focus-Description": "Nikon TE2000 focus adapter", "FilterBlock-State": "5", "520 AO-Demo": "No", "COM3-DelayBetweenCharsMs": "0.0000", "470 Shutter-Volts": "0.0000", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "PRIME-PP 2 THRESHOLD": "125", "PRIME-PP 4 THRESHOLD": "75", "XYStage-Acceleration": "4.0000", "470 Shutter-Demo": "No", "PRIME-Name": "PMPCIECam00", "640-DA Device": "640 Shutter", "405 AO-Block voltage": "false", "ElapsedTime-ms": 1393915, "PRIME-Offset": "100", "445 AO-IOChannel": "Dev1/ao1", "640 AO-MaxVolts": "5.0000", "PositionIndex": 34, "PRIME-PP 1 ENABLED": "Yes", "COM1-BaudRate": "9600", "640-State": "0", "COM3-Verbose": "1", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "LudlController-TransmissionDelay": "0", "PRIME-FullWellCapacity": "62000", "PRIME-SMARTStreamingEnabled": "No", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "640 Shutter-Description": "NI DAC", "528-Description": "DA device that is used as a shutter", "COM4-Description": "Serial port driver (boost:asio)", "405 Shutter-Description": "NI DAC", "TE2000-Name": "TE2000", "528 Shutter-Description": "NI DAC", "COM9-Description": "Serial port driver (boost:asio)", "LudlController-Name": "LudlController", "445 Shutter-TriggerSequenceLength": "1000", "PRIME-SerialNumber": "A18B203008", "445 AO-Volts": "0.0000", "PRIME-X-dimension": "1200", "PRIME-ShutterCloseDelay": "0", "XLIGHT Disk slider-State": "2", "Position": "Default", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 AO-IOChannel": "Dev1/ao5", "520 AO-IOChannel": "Dev1/ao4", "528 AO-TriggerSequenceLength": "1000", "405 Shutter-MaxVolts": "5.0000", "470-DA Device": "470 Shutter", "PRIME-FTCapable": "No", "Core-ChannelGroup": "Channels", "555 AO-SupportsTriggering": "0", "PRIME-Y-dimension": "1200", "Frame": 0, "555 Shutter-Sequenceable": "1", "PRIME-Timing-ExposureTimeNs": "499998000.0000", "Core-ImageProcessor": "", "528-State": "0", "520 AO-Block voltage": "false", "XLIGHT Excitation Wheel-Label": "CFP/YFP", "640 Shutter-Demo": "No", "COM3-StopBits": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "520 Shutter-IOChannel": "Dev1/ao11", "Width": 1200, "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-SupportsTriggering": "0", "640 AO-TriggerSampleRate": "100000", "520-Description": "DA device that is used as a shutter", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "405 Shutter-Sequenceable": "1", "405 Shutter-SupportsTriggering": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-TriggerSampleRate": "100000", "640 Shutter-MaxVolts": "5.0000", "555 AO-MaxVolts": "5.0000", "520 AO-TriggerSequenceLength": "1000", "COM3-BaudRate": "9600", "COM9-BaudRate": "9600", "Vincent-D3-Channel #": "Ch. #1", "PRIME-PMode": "Normal", "OpticalPath-Label": "Left(100)", "BitDepth": 16, "555 AO-TriggerSequenceLength": "1000", "445 Shutter-Description": "NI DAC", "405 AO-Sequenceable": "1", "PRIME-AcquisitionMethod": "Callbacks", "555 AO-Name": "AnalogIO", "PRIME-ShutterMode": "Pre-Exposure", "COM3-Handshaking": "Off", "445 AO-SupportsTriggering": "0", "ROI": "0-0-1200-1200", "COM9-Verbose": "1", "PRIME-Color": "OFF", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "PRIME-ShutterOpenDelay": "0", "COM1-Parity": "None", "Slice": 5, "SlicePosition": 0, "COM9-DelayBetweenCharsMs": "0.0000", "555-Name": "DA Shutter", "PIEZO-LudlSingleAxisName": "B", "XLIGHT Emission Wheel-Label": "565/24(G)", "640 Shutter-Name": "AnalogIO", "640 AO-Sequenceable": "1", "520 Shutter-Demo": "No", "528 Shutter-Demo": "No", "470-Name": "DA Shutter", "520-DA Device": "520 Shutter", "XYStage-Description": "Ludl XY stage driver adapter", "445 Shutter-MinVolts": "0.0000", "528 AO-TriggerSampleRate": "100000", "405 AO-MinVolts": "0.0000", "405 Shutter-TriggerSequenceLength": "1000", "Height": 1200, "555 AO-Block voltage": "false", "640 Shutter-Volts": "0.0000", "PixelType": "GRAY16", "445 Shutter-Block voltage": "false", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "520 Shutter-TriggerSequenceLength": "1000", "COM9-Name": "COM9", "Core-SLM": "", "555 AO-Volts": "0.0000", "555 Shutter-Block voltage": "false", "XYStage-Speed": "1800.0000", "520 AO-MaxVolts": "5.0000", "528 Shutter-SupportsTriggering": "0", "COM1-Description": "Serial port driver (boost:asio)", "PRIME-MetadataEnabled": "No", "520 Shutter-MinVolts": "0.0000", "470 Shutter-Sequenceable": "1", "PRIME-PreampDelay": "0", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-MinVolts": "0.0000", "ChannelIndex": 0, "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Core-AutoShutter": "1", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "Exposure-ms": 320, "PRIME-PP 2 ENABLED": "Yes", "640 Shutter-TriggerSampleRate": "100000", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "470 Shutter-IOChannel": "Dev1/ao10", "405 AO-Demo": "No", "PixelSizeUm": 1.1, "470 AO-Demo": "No", "470 Shutter-MaxVolts": "5.0000", "Vincent-D3-Name": "Vincent-D3", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FileName": "img_000000000_Il-G_005.tif", "LudlController-Reset": "Operate", "PRIME-Exposure": "320.00", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "445-Name": "DA Shutter", "555 Shutter-Name": "AnalogIO", "520 Shutter-TriggerSampleRate": "100000", "445 Shutter-IOChannel": "Dev1/ao9", "PRIME-TransposeCorrection": "0", "XYStage-StepSize": "0.1000", "445-Description": "DA device that is used as a shutter", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 AO-IOChannel": "Dev1/ao7", "520 Shutter-Name": "AnalogIO", "COM3-DataBits": "8", "640 AO-Volts": "0.0000", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "555 Shutter-IOChannel": "Dev1/ao13", "WaitInterval": null, "520 Shutter-Sequenceable": "1", "YPositionUm": 545.6, "445-State": "0", "445-DA Device": "445 Shutter", "PRIME-CircularBufferAutoSize": "ON", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "555 AO-TriggerSampleRate": "100000", "445 Shutter-Volts": "0.0000", "XLIGHT Hub-Port": "COM3", "Core-AutoFocus": "", "COM4-Handshaking": "Off", "528 Shutter-MinVolts": "0.0000", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "445 Shutter-Name": "AnalogIO", "528 Shutter-Sequenceable": "1", "XYStage-TransposeMirrorX": "0", "XYStage-TransposeMirrorY": "0", "PRIME-ReadoutRate": "100MHz 16bit", "XYStage-Name": "Stage", "640 AO-SupportsTriggering": "0", "640-Name": "DA Shutter", "640 Shutter-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "640 AO-Block voltage": "false", "445 Shutter-Sequenceable": "1", "470 AO-MaxVolts": "5.0000", "COM4-BaudRate": "9600", "470-Description": "DA device that is used as a shutter", "520 Shutter-Description": "NI DAC", "PRIME-CCDTemperature": "-14.8500", "Time": "2019-12-19 09:59:24 -0500", "640 AO-TriggerSequenceLength": "1000", "470 AO-Name": "AnalogIO", "XLIGHT Disk slider-Label": "Disk Pos 40um", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "Binning": "1x1", "TE2000-ModelType": "1", "COM3-AnswerTimeout": "500.0000", "555 Shutter-SupportsTriggering": "0", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "TE2000-Port": "COM4", "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Description": "NI DAC", "COM4-AnswerTimeout": "500.0000", "COM1-DelayBetweenCharsMs": "0.0000", "FilterBlock-Label": "Confocal", "445 AO-Name": "AnalogIO", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-Timing-ReadoutTimeNs": "24672000.0000", "528 AO-Demo": "No", "520 AO-Sequenceable": "1", "555-Description": "DA device that is used as a shutter", "405-DA Device": "405 Shutter", "405 AO-TriggerSequenceLength": "1000", "640 AO-MinVolts": "0.0000", "640 Shutter-Block voltage": "false", "FrameIndex": 0, "PRIME-Port": "CMOS", "555 AO-MinVolts": "0.0000", "COM3-Description": "Serial port driver (boost:asio)", "528 AO-Description": "NI DAC", "555 AO-IOChannel": "Dev1/ao6", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "555 AO-TriggerInputLine": "/Dev1/PFI0", "PRIME-ActualInterval-ms": "390.5200", "LudlController-Port": "COM9", "445 Shutter-Demo": "No", "PRIME-Trigger-Expose Out-Mux": "1", "SliceIndex": 5, "PRIME-CCDTemperatureSetPoint": "-15.0000", "405 Shutter-MinVolts": "0.0000", "PRIME-ExposeOutMode": "First Row", "AxisPositions": null, "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Volts": "0.0000", "528-Name": "DA Shutter", "555-State": "0", "XLIGHT Dichroic-Label": "CFP/YFP", "445 AO-Sequenceable": "1", "405-Name": "DA Shutter", "405 AO-IOChannel": "Dev1/ao0", "Core-Camera": "PRIME", "Focus-Name": "Focus", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "405 Shutter-IOChannel": "Dev1/ao8", "640 AO-Demo": "No", "640 Shutter-SupportsTriggering": "0", "445 AO-Demo": "No", "555-DA Device": "555 Shutter", "470 Shutter-Description": "NI DAC", "528 AO-Volts": "0.0000", "COM1-DataBits": "8", "Vincent-D3-Address": "x", "520 AO-SupportsTriggering": "0", "405-Description": "DA device that is used as a shutter", "640 Shutter-MinVolts": "0.0000", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "405 AO-Name": "AnalogIO", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "640 AO-TriggerInputLine": "/Dev1/PFI0", "405 AO-TriggerInputLine": "/Dev1/PFI0", "640-Description": "DA device that is used as a shutter", "445 Shutter-TriggerSampleRate": "100000", "470 Shutter-TriggerSequenceLength": "1000", "COM1-StopBits": "1", "520 Shutter-SupportsTriggering": "0", "405-State": "0", "Core-Initialize": "1", "XLIGHT Emission Wheel-State": "3", "520 AO-Description": "NI DAC", "528 AO-TriggerInputLine": "/Dev1/PFI0", "445 Shutter-MaxVolts": "5.0000", "COM9-Handshaking": "Off", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "XLIGHT Spin motor-Label": "On (spinning)", "PRIME-PP 5 ENABLED": "No", "UUID": "f2672945-9476-40c7-bfec-9796babc6cc9", "PRIME-TriggerMode": "Internal Trigger", "COM1-Handshaking": "Off", "PRIME-Timing-PostTriggerDelayNs": "49344000.0000", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "Focus-Position": "17741.5000", "528 Shutter-Block voltage": "false", "Channel": "Il-G", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "555 Shutter-Demo": "No", "405 Shutter-Demo": "No", "528 AO-Name": "AnalogIO", "520-State": "1", "Core-Galvo": "", "405 Shutter-TriggerSampleRate": "100000", "Focus-FocusStepSize_(nm)": "100", "PRIME-PixelType": "16bit", "COM4-DataBits": "8", "470 AO-IOChannel": "Dev1/ao2", "520 AO-MinVolts": "0.0000", "COM9-Parity": "None", "PRIME-CircularBufferEnabled": "ON", "PRIME-PP 6 Reset": "No", "640 AO-Description": "NI DAC", "Source": "PRIME", "445 AO-MinVolts": "0.0000", "PIEZO-Name": "Stage", "445 AO-Description": "NI DAC", "PRIME-CircularBufferFrameCount": "46", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "520 AO-Volts": "4.5000", "528 Shutter-TriggerSampleRate": "100000", "XPositionUm": -1324.9, "OpticalPath-State": "4", "PRIME-PP 3 THRESHOLD": "80", "PRIME-PVCAM Version": "3.7.1", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Gain": "1-HDR", "640 Shutter-TriggerSequenceLength": "1000", "528 AO-SupportsTriggering": "0", "445 AO-TriggerInputLine": "/Dev1/PFI0", "OpticalPath-Name": "OpticalPath", "PRIME-PP 4 ENABLED": "Yes", "PRIME-PVCAM Adapter Version": "1.3.29", "COM3-Name": "COM3", "405 AO-MaxVolts": "5.0000", "520 Shutter-Block voltage": "false", "PRIME-Timing-ClearingTimeNs": "49344000.0000", "Core-Focus": "PIEZO", "528 AO-Block voltage": "false", "TE2000-Description": "Nikon TE2000 hub", "528 AO-MinVolts": "0.0000", "COM4-Parity": "None", "Summary": { "Slices": 11, "ChContrastMin": [ 0, 0, 0, 0, 0, 0 ], "PixelAspect": 1, "SlicesFirst": true, "Interval_ms": 0, "Time": "2019-12-19 09:59:21 -0500", "Prefix": "seq_13", "InitialPositionList": [ { "Label": "Pos0", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.21], "Focus": [17741.5], "XYStage": [ 44542.200000000004, 31636.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos1", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.22], "Focus": [17741.4], "XYStage": [ 43801.100000000006, 31637.9 ] }, "GridColumnIndex": 0 }, { "Label": "Pos2", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.5], "XYStage": [ 38561.3, 32040.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos3", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.185], "Focus": [17741.3], "XYStage": [ 37698.4, 32041.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos4", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.16], "Focus": [17741.5], "XYStage": [ 32375.4, 31758.9 ] }, "GridColumnIndex": 0 }, { "Label": "Pos5", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.17000000000002], "Focus": [17741.3], "XYStage": [ 31770.7, 31758.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos6", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.4], "XYStage": [ 26536.800000000003, 31068.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos7", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.205], "Focus": [17741.4], "XYStage": [ 25420.2, 31068.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos8", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.85], "Focus": [17741.3], "XYStage": [ 21059, 31508 ] }, "GridColumnIndex": 0 }, { "Label": "Pos9", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.82], "Focus": [17741.3], "XYStage": [ 19731.9, 31506.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos10", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.79], "Focus": [17741.4], "XYStage": [ 14528.5, 32007.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos11", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.83], "Focus": [17741.2], "XYStage": [ 13830.300000000001, 32009.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos12", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.79], "Focus": [17741.3], "XYStage": [ 8775.300000000001, 32017.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos13", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.815], "Focus": [17741.3], "XYStage": [ 8109.200000000001, 32008.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos14", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.805], "Focus": [17741.3], "XYStage": [ 3483.3, 31908.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos15", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.82], "Focus": [17741.2], "XYStage": [ 2686.8, 31903.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos16", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [211.48000000000002], "Focus": [17741.1], "XYStage": [ -1978.9, 31973.600000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos17", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [211.51500000000001], "Focus": [17741.1], "XYStage": [ -2957.8, 31982.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos18", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.195], "Focus": [17741.5], "XYStage": [ 47747.4, -576.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos19", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.23000000000002], "Focus": [17741.6], "XYStage": [ 47053.9, -576.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos20", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.205], "Focus": [17741.4], "XYStage": [ 41871.200000000004, -479.8 ] }, "GridColumnIndex": 0 }, { "Label": "Pos21", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.4], "XYStage": [ 40882.600000000006, -392 ] }, "GridColumnIndex": 0 }, { "Label": "Pos22", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.24], "Focus": [17741.4], "XYStage": [ 35905.6, -135.3 ] }, "GridColumnIndex": 0 }, { "Label": "Pos23", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.195], "Focus": [17741.5], "XYStage": [ 34990.9, -132.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos24", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.46], "Focus": [17741.4], "XYStage": [ 29678.100000000002, -958.1 ] }, "GridColumnIndex": 0 }, { "Label": "Pos25", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.44], "Focus": [17741.5], "XYStage": [ 28983.600000000002, -939.4000000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos26", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.475], "Focus": [17741.5], "XYStage": [ 23019.7, -122 ] }, "GridColumnIndex": 0 }, { "Label": "Pos27", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.44], "Focus": [17741.7], "XYStage": [ 22428, -116.80000000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos28", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.46], "Focus": [17741.7], "XYStage": [ 16364.900000000001, -181.10000000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos29", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.48000000000002], "Focus": [17741.5], "XYStage": [ 15712.1, -180 ] }, "GridColumnIndex": 0 }, { "Label": "Pos30", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.45000000000002], "Focus": [17741.7], "XYStage": [ 10346.1, 77.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos31", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.49], "Focus": [17741.4], "XYStage": [ 9755.5, 85.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos32", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.475], "Focus": [17741.5], "XYStage": [ 4775.5, 293.90000000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos33", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.445], "Focus": [17741.4], "XYStage": [ 3954.9, 293.90000000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos34", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.455], "Focus": [17741.4], "XYStage": [ -1324.9, 545.6 ] }, "GridColumnIndex": 0 }, { "Label": "Pos35", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.445], "Focus": [17741.3], "XYStage": [ -2097, 576.6 ] }, "GridColumnIndex": 0 } ], "z-step_um": 10, "GridRow": 0, "BitDepth": 16, "Depth": 2, "Channels": 6, "Source": "Micro-Manager", "ChColors": [ -16711681, -256, -65281, -1, -1, -16776961 ], "MetadataVersion": 10, "KeepShutterOpenChannels": true, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536, 65536 ], "CustomIntervals_ms": [], "Height": 1200, "MicroManagerVersion": "1.4.23 20180219", "GridColumn": 0, "UUID": "18808e7c-f2e6-47b3-90d8-1d779ce87dbd", "PixelType": "GRAY16", "Width": 1200, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\Yan\\20191218-YC113-S7S8-miseq", "Frames": 1, "Comment": "", "UserName": "Nikon", "PixelSize_um": 1.1, "ROI": [ 0, 0, 1200, 1200 ], "Date": "2019-12-19", "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC", "P-DAPI-high" ], "KeepShutterOpenSlices": true, "IJType": 1, "ComputerName": "ZadorConfocal2", "Positions": 36, "PositionIndex": 34, "CameraTimeout": 20000, "TimeFirst": false }, "640 AO-Name": "AnalogIO", "Vincent-D3-Command": "Close" }, "FrameKey-0-0-6": { "COM4-StopBits": "1", "528 AO-MaxVolts": "5.0000", "XLIGHT Excitation Wheel-State": "1", "470 Shutter-Name": "AnalogIO", "Core-Shutter": "520", "555 AO-Demo": "No", "XLIGHT Dichroic-State": "2", "XYStage-StepSize-Y": "0.1000", "ZPositionUm": 228.455, "XYStage-StepSize-X": "0.1000", "PRIME-FirmwareVersion": "20.28", "PRIME-ClearCycles": "2", "520-Name": "DA Shutter", "405 Shutter-Name": "AnalogIO", "PIEZO-StepSize": "0.0050", "PRIME-ClearMode": "Pre-Exposure", "470 AO-MinVolts": "0.0000", "405 AO-SupportsTriggering": "1", "CameraChannelIndex": 0, "XYStage-StartSpeed": "500.0000", "COM9-StopBits": "2", "405 AO-Description": "NI DAC", "COM4-DelayBetweenCharsMs": "0.0000", "445 AO-Block voltage": "false", "520 AO-TriggerInputLine": "/Dev1/PFI0", "555 AO-Description": "NI DAC", "520 AO-TriggerSampleRate": "100000", "COM4-Verbose": "1", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM4-Name": "COM4", "405 Shutter-Volts": "0.0000", "Vincent-D3-Port": "COM1", "PRIME-PP 4 MIN ADU AFFECTED": "200", "470 Shutter-MinVolts": "0.0000", "PRIME-PP 3 ENABLED": "Yes", "470 AO-Description": "NI DAC", "PRIME-Description": "PVCAM API device adapter", "520 Shutter-MaxVolts": "5.0000", "520 Shutter-Volts": "5.0000", "528 AO-Sequenceable": "1", "PRIME-Binning": "1x1", "555 Shutter-MaxVolts": "5.0000", "528 Shutter-TriggerSequenceLength": "1000", "405 Shutter-Block voltage": "false", "XLIGHT Spin motor-State": "1", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "Vincent-D3-Time to open (ms)": "35.0000", "Vincent-D3-Time to close (ms)": "35.0000", "445 AO-TriggerSampleRate": "100000", "640 Shutter-IOChannel": "Dev1/ao14", "555 AO-Sequenceable": "1", "520 AO-Name": "AnalogIO", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "470 AO-Block voltage": "false", "COM1-Verbose": "1", "COM9-AnswerTimeout": "10000.0000", "528 Shutter-Name": "AnalogIO", "PRIME-Trigger Timeout (secs)": "10", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "NextFrame": 0, "470 AO-TriggerSequenceLength": "1000", "PRIME-TransposeXY": "0", "445 AO-TriggerSequenceLength": "1000", "COM1-Name": "COM1", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "COM9-DataBits": "8", "COM1-AnswerTimeout": "500.0000", "Camera": "", "TE2000-Version": "V2.50-V2.50-V2.10", "528 Shutter-Volts": "0.0000", "XLIGHT Hub-Name": "XLIGHT Hub", "PIEZO-Description": "Ludl stage driver adapter", "PositionName": "Pos34", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSampleRate": "100000", "PRIME-FanSpeedSetpoint": "High", "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "405 AO-TriggerSampleRate": "100000", "470 Shutter-Block voltage": "false", "470-State": "0", "COM3-Parity": "None", "405 AO-Volts": "0.0000", "PRIME-TransposeMirrorY": "0", "PRIME-ChipName": "GS144BSI", "470 AO-Volts": "0.0000", "PRIME-TransposeMirrorX": "0", "445 Shutter-SupportsTriggering": "0", "470 Shutter-SupportsTriggering": "0", "XLIGHT Hub-Description": "XLIGHT hub", "PRIME-PreampOffLimit": "10000", "470 AO-Sequenceable": "1", "528 Shutter-MaxVolts": "5.0000", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "Core-XYStage": "XYStage", "470 AO-TriggerSampleRate": "100000", "PRIME-Timing-PreTriggerDelayNs": "24672000.0000", "528-DA Device": "528 Shutter", "Focus-Description": "Nikon TE2000 focus adapter", "FilterBlock-State": "5", "520 AO-Demo": "No", "COM3-DelayBetweenCharsMs": "0.0000", "470 Shutter-Volts": "0.0000", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "PRIME-PP 2 THRESHOLD": "125", "PRIME-PP 4 THRESHOLD": "75", "XYStage-Acceleration": "4.0000", "470 Shutter-Demo": "No", "PRIME-Name": "PMPCIECam00", "640-DA Device": "640 Shutter", "405 AO-Block voltage": "false", "ElapsedTime-ms": 1394475, "PRIME-Offset": "100", "445 AO-IOChannel": "Dev1/ao1", "640 AO-MaxVolts": "5.0000", "PositionIndex": 34, "PRIME-PP 1 ENABLED": "Yes", "COM1-BaudRate": "9600", "640-State": "0", "COM3-Verbose": "1", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "LudlController-TransmissionDelay": "0", "PRIME-FullWellCapacity": "62000", "PRIME-SMARTStreamingEnabled": "No", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "640 Shutter-Description": "NI DAC", "528-Description": "DA device that is used as a shutter", "COM4-Description": "Serial port driver (boost:asio)", "405 Shutter-Description": "NI DAC", "TE2000-Name": "TE2000", "528 Shutter-Description": "NI DAC", "COM9-Description": "Serial port driver (boost:asio)", "LudlController-Name": "LudlController", "445 Shutter-TriggerSequenceLength": "1000", "PRIME-SerialNumber": "A18B203008", "445 AO-Volts": "0.0000", "PRIME-X-dimension": "1200", "PRIME-ShutterCloseDelay": "0", "XLIGHT Disk slider-State": "2", "Position": "Default", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 AO-IOChannel": "Dev1/ao5", "520 AO-IOChannel": "Dev1/ao4", "528 AO-TriggerSequenceLength": "1000", "405 Shutter-MaxVolts": "5.0000", "470-DA Device": "470 Shutter", "PRIME-FTCapable": "No", "Core-ChannelGroup": "Channels", "555 AO-SupportsTriggering": "0", "PRIME-Y-dimension": "1200", "Frame": 0, "555 Shutter-Sequenceable": "1", "PRIME-Timing-ExposureTimeNs": "499998000.0000", "Core-ImageProcessor": "", "528-State": "0", "520 AO-Block voltage": "false", "XLIGHT Excitation Wheel-Label": "CFP/YFP", "640 Shutter-Demo": "No", "COM3-StopBits": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "520 Shutter-IOChannel": "Dev1/ao11", "Width": 1200, "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-SupportsTriggering": "0", "640 AO-TriggerSampleRate": "100000", "520-Description": "DA device that is used as a shutter", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "405 Shutter-Sequenceable": "1", "405 Shutter-SupportsTriggering": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-TriggerSampleRate": "100000", "640 Shutter-MaxVolts": "5.0000", "555 AO-MaxVolts": "5.0000", "520 AO-TriggerSequenceLength": "1000", "COM3-BaudRate": "9600", "COM9-BaudRate": "9600", "Vincent-D3-Channel #": "Ch. #1", "PRIME-PMode": "Normal", "OpticalPath-Label": "Left(100)", "BitDepth": 16, "555 AO-TriggerSequenceLength": "1000", "445 Shutter-Description": "NI DAC", "405 AO-Sequenceable": "1", "PRIME-AcquisitionMethod": "Callbacks", "555 AO-Name": "AnalogIO", "PRIME-ShutterMode": "Pre-Exposure", "COM3-Handshaking": "Off", "445 AO-SupportsTriggering": "0", "ROI": "0-0-1200-1200", "COM9-Verbose": "1", "PRIME-Color": "OFF", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "PRIME-ShutterOpenDelay": "0", "COM1-Parity": "None", "Slice": 6, "SlicePosition": 10, "COM9-DelayBetweenCharsMs": "0.0000", "555-Name": "DA Shutter", "PIEZO-LudlSingleAxisName": "B", "XLIGHT Emission Wheel-Label": "565/24(G)", "640 Shutter-Name": "AnalogIO", "640 AO-Sequenceable": "1", "520 Shutter-Demo": "No", "528 Shutter-Demo": "No", "470-Name": "DA Shutter", "520-DA Device": "520 Shutter", "XYStage-Description": "Ludl XY stage driver adapter", "445 Shutter-MinVolts": "0.0000", "528 AO-TriggerSampleRate": "100000", "405 AO-MinVolts": "0.0000", "405 Shutter-TriggerSequenceLength": "1000", "Height": 1200, "555 AO-Block voltage": "false", "640 Shutter-Volts": "0.0000", "PixelType": "GRAY16", "445 Shutter-Block voltage": "false", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "520 Shutter-TriggerSequenceLength": "1000", "COM9-Name": "COM9", "Core-SLM": "", "555 AO-Volts": "0.0000", "555 Shutter-Block voltage": "false", "XYStage-Speed": "1800.0000", "520 AO-MaxVolts": "5.0000", "528 Shutter-SupportsTriggering": "0", "COM1-Description": "Serial port driver (boost:asio)", "PRIME-MetadataEnabled": "No", "520 Shutter-MinVolts": "0.0000", "470 Shutter-Sequenceable": "1", "PRIME-PreampDelay": "0", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-MinVolts": "0.0000", "ChannelIndex": 0, "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Core-AutoShutter": "1", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "Exposure-ms": 320, "PRIME-PP 2 ENABLED": "Yes", "640 Shutter-TriggerSampleRate": "100000", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "470 Shutter-IOChannel": "Dev1/ao10", "405 AO-Demo": "No", "PixelSizeUm": 1.1, "470 AO-Demo": "No", "470 Shutter-MaxVolts": "5.0000", "Vincent-D3-Name": "Vincent-D3", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FileName": "img_000000000_Il-G_006.tif", "LudlController-Reset": "Operate", "PRIME-Exposure": "320.00", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "445-Name": "DA Shutter", "555 Shutter-Name": "AnalogIO", "520 Shutter-TriggerSampleRate": "100000", "445 Shutter-IOChannel": "Dev1/ao9", "PRIME-TransposeCorrection": "0", "XYStage-StepSize": "0.1000", "445-Description": "DA device that is used as a shutter", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 AO-IOChannel": "Dev1/ao7", "520 Shutter-Name": "AnalogIO", "COM3-DataBits": "8", "640 AO-Volts": "0.0000", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "555 Shutter-IOChannel": "Dev1/ao13", "WaitInterval": null, "520 Shutter-Sequenceable": "1", "YPositionUm": 545.6, "445-State": "0", "445-DA Device": "445 Shutter", "PRIME-CircularBufferAutoSize": "ON", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "555 AO-TriggerSampleRate": "100000", "445 Shutter-Volts": "0.0000", "XLIGHT Hub-Port": "COM3", "Core-AutoFocus": "", "COM4-Handshaking": "Off", "528 Shutter-MinVolts": "0.0000", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "445 Shutter-Name": "AnalogIO", "528 Shutter-Sequenceable": "1", "XYStage-TransposeMirrorX": "0", "XYStage-TransposeMirrorY": "0", "PRIME-ReadoutRate": "100MHz 16bit", "XYStage-Name": "Stage", "640 AO-SupportsTriggering": "0", "640-Name": "DA Shutter", "640 Shutter-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "640 AO-Block voltage": "false", "445 Shutter-Sequenceable": "1", "470 AO-MaxVolts": "5.0000", "COM4-BaudRate": "9600", "470-Description": "DA device that is used as a shutter", "520 Shutter-Description": "NI DAC", "PRIME-CCDTemperature": "-14.8500", "Time": "2019-12-19 09:59:24 -0500", "640 AO-TriggerSequenceLength": "1000", "470 AO-Name": "AnalogIO", "XLIGHT Disk slider-Label": "Disk Pos 40um", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "Binning": "1x1", "TE2000-ModelType": "1", "COM3-AnswerTimeout": "500.0000", "555 Shutter-SupportsTriggering": "0", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "TE2000-Port": "COM4", "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Description": "NI DAC", "COM4-AnswerTimeout": "500.0000", "COM1-DelayBetweenCharsMs": "0.0000", "FilterBlock-Label": "Confocal", "445 AO-Name": "AnalogIO", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-Timing-ReadoutTimeNs": "24672000.0000", "528 AO-Demo": "No", "520 AO-Sequenceable": "1", "555-Description": "DA device that is used as a shutter", "405-DA Device": "405 Shutter", "405 AO-TriggerSequenceLength": "1000", "640 AO-MinVolts": "0.0000", "640 Shutter-Block voltage": "false", "FrameIndex": 0, "PRIME-Port": "CMOS", "555 AO-MinVolts": "0.0000", "COM3-Description": "Serial port driver (boost:asio)", "528 AO-Description": "NI DAC", "555 AO-IOChannel": "Dev1/ao6", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "555 AO-TriggerInputLine": "/Dev1/PFI0", "PRIME-ActualInterval-ms": "390.5200", "LudlController-Port": "COM9", "445 Shutter-Demo": "No", "PRIME-Trigger-Expose Out-Mux": "1", "SliceIndex": 6, "PRIME-CCDTemperatureSetPoint": "-15.0000", "405 Shutter-MinVolts": "0.0000", "PRIME-ExposeOutMode": "First Row", "AxisPositions": null, "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Volts": "0.0000", "528-Name": "DA Shutter", "555-State": "0", "XLIGHT Dichroic-Label": "CFP/YFP", "445 AO-Sequenceable": "1", "405-Name": "DA Shutter", "405 AO-IOChannel": "Dev1/ao0", "Core-Camera": "PRIME", "Focus-Name": "Focus", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "405 Shutter-IOChannel": "Dev1/ao8", "640 AO-Demo": "No", "640 Shutter-SupportsTriggering": "0", "445 AO-Demo": "No", "555-DA Device": "555 Shutter", "470 Shutter-Description": "NI DAC", "528 AO-Volts": "0.0000", "COM1-DataBits": "8", "Vincent-D3-Address": "x", "520 AO-SupportsTriggering": "0", "405-Description": "DA device that is used as a shutter", "640 Shutter-MinVolts": "0.0000", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "405 AO-Name": "AnalogIO", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "640 AO-TriggerInputLine": "/Dev1/PFI0", "405 AO-TriggerInputLine": "/Dev1/PFI0", "640-Description": "DA device that is used as a shutter", "445 Shutter-TriggerSampleRate": "100000", "470 Shutter-TriggerSequenceLength": "1000", "COM1-StopBits": "1", "520 Shutter-SupportsTriggering": "0", "405-State": "0", "Core-Initialize": "1", "XLIGHT Emission Wheel-State": "3", "520 AO-Description": "NI DAC", "528 AO-TriggerInputLine": "/Dev1/PFI0", "445 Shutter-MaxVolts": "5.0000", "COM9-Handshaking": "Off", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "XLIGHT Spin motor-Label": "On (spinning)", "PRIME-PP 5 ENABLED": "No", "UUID": "9ebd4b01-934b-4ada-b408-65cd38ee7c47", "PRIME-TriggerMode": "Internal Trigger", "COM1-Handshaking": "Off", "PRIME-Timing-PostTriggerDelayNs": "49344000.0000", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "Focus-Position": "17741.5000", "528 Shutter-Block voltage": "false", "Channel": "Il-G", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "555 Shutter-Demo": "No", "405 Shutter-Demo": "No", "528 AO-Name": "AnalogIO", "520-State": "1", "Core-Galvo": "", "405 Shutter-TriggerSampleRate": "100000", "Focus-FocusStepSize_(nm)": "100", "PRIME-PixelType": "16bit", "COM4-DataBits": "8", "470 AO-IOChannel": "Dev1/ao2", "520 AO-MinVolts": "0.0000", "COM9-Parity": "None", "PRIME-CircularBufferEnabled": "ON", "PRIME-PP 6 Reset": "No", "640 AO-Description": "NI DAC", "Source": "PRIME", "445 AO-MinVolts": "0.0000", "PIEZO-Name": "Stage", "445 AO-Description": "NI DAC", "PRIME-CircularBufferFrameCount": "46", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "520 AO-Volts": "4.5000", "528 Shutter-TriggerSampleRate": "100000", "XPositionUm": -1324.9, "OpticalPath-State": "4", "PRIME-PP 3 THRESHOLD": "80", "PRIME-PVCAM Version": "3.7.1", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Gain": "1-HDR", "640 Shutter-TriggerSequenceLength": "1000", "528 AO-SupportsTriggering": "0", "445 AO-TriggerInputLine": "/Dev1/PFI0", "OpticalPath-Name": "OpticalPath", "PRIME-PP 4 ENABLED": "Yes", "PRIME-PVCAM Adapter Version": "1.3.29", "COM3-Name": "COM3", "405 AO-MaxVolts": "5.0000", "520 Shutter-Block voltage": "false", "PRIME-Timing-ClearingTimeNs": "49344000.0000", "Core-Focus": "PIEZO", "528 AO-Block voltage": "false", "TE2000-Description": "Nikon TE2000 hub", "528 AO-MinVolts": "0.0000", "COM4-Parity": "None", "Summary": { "Slices": 11, "ChContrastMin": [ 0, 0, 0, 0, 0, 0 ], "PixelAspect": 1, "SlicesFirst": true, "Interval_ms": 0, "Time": "2019-12-19 09:59:21 -0500", "Prefix": "seq_13", "InitialPositionList": [ { "Label": "Pos0", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.21], "Focus": [17741.5], "XYStage": [ 44542.200000000004, 31636.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos1", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.22], "Focus": [17741.4], "XYStage": [ 43801.100000000006, 31637.9 ] }, "GridColumnIndex": 0 }, { "Label": "Pos2", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.5], "XYStage": [ 38561.3, 32040.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos3", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.185], "Focus": [17741.3], "XYStage": [ 37698.4, 32041.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos4", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.16], "Focus": [17741.5], "XYStage": [ 32375.4, 31758.9 ] }, "GridColumnIndex": 0 }, { "Label": "Pos5", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.17000000000002], "Focus": [17741.3], "XYStage": [ 31770.7, 31758.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos6", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.4], "XYStage": [ 26536.800000000003, 31068.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos7", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.205], "Focus": [17741.4], "XYStage": [ 25420.2, 31068.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos8", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.85], "Focus": [17741.3], "XYStage": [ 21059, 31508 ] }, "GridColumnIndex": 0 }, { "Label": "Pos9", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.82], "Focus": [17741.3], "XYStage": [ 19731.9, 31506.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos10", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.79], "Focus": [17741.4], "XYStage": [ 14528.5, 32007.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos11", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.83], "Focus": [17741.2], "XYStage": [ 13830.300000000001, 32009.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos12", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.79], "Focus": [17741.3], "XYStage": [ 8775.300000000001, 32017.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos13", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.815], "Focus": [17741.3], "XYStage": [ 8109.200000000001, 32008.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos14", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.805], "Focus": [17741.3], "XYStage": [ 3483.3, 31908.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos15", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.82], "Focus": [17741.2], "XYStage": [ 2686.8, 31903.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos16", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [211.48000000000002], "Focus": [17741.1], "XYStage": [ -1978.9, 31973.600000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos17", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [211.51500000000001], "Focus": [17741.1], "XYStage": [ -2957.8, 31982.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos18", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.195], "Focus": [17741.5], "XYStage": [ 47747.4, -576.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos19", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.23000000000002], "Focus": [17741.6], "XYStage": [ 47053.9, -576.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos20", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.205], "Focus": [17741.4], "XYStage": [ 41871.200000000004, -479.8 ] }, "GridColumnIndex": 0 }, { "Label": "Pos21", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.4], "XYStage": [ 40882.600000000006, -392 ] }, "GridColumnIndex": 0 }, { "Label": "Pos22", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.24], "Focus": [17741.4], "XYStage": [ 35905.6, -135.3 ] }, "GridColumnIndex": 0 }, { "Label": "Pos23", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.195], "Focus": [17741.5], "XYStage": [ 34990.9, -132.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos24", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.46], "Focus": [17741.4], "XYStage": [ 29678.100000000002, -958.1 ] }, "GridColumnIndex": 0 }, { "Label": "Pos25", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.44], "Focus": [17741.5], "XYStage": [ 28983.600000000002, -939.4000000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos26", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.475], "Focus": [17741.5], "XYStage": [ 23019.7, -122 ] }, "GridColumnIndex": 0 }, { "Label": "Pos27", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.44], "Focus": [17741.7], "XYStage": [ 22428, -116.80000000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos28", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.46], "Focus": [17741.7], "XYStage": [ 16364.900000000001, -181.10000000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos29", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.48000000000002], "Focus": [17741.5], "XYStage": [ 15712.1, -180 ] }, "GridColumnIndex": 0 }, { "Label": "Pos30", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.45000000000002], "Focus": [17741.7], "XYStage": [ 10346.1, 77.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos31", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.49], "Focus": [17741.4], "XYStage": [ 9755.5, 85.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos32", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.475], "Focus": [17741.5], "XYStage": [ 4775.5, 293.90000000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos33", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.445], "Focus": [17741.4], "XYStage": [ 3954.9, 293.90000000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos34", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.455], "Focus": [17741.4], "XYStage": [ -1324.9, 545.6 ] }, "GridColumnIndex": 0 }, { "Label": "Pos35", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.445], "Focus": [17741.3], "XYStage": [ -2097, 576.6 ] }, "GridColumnIndex": 0 } ], "z-step_um": 10, "GridRow": 0, "BitDepth": 16, "Depth": 2, "Channels": 6, "Source": "Micro-Manager", "ChColors": [ -16711681, -256, -65281, -1, -1, -16776961 ], "MetadataVersion": 10, "KeepShutterOpenChannels": true, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536, 65536 ], "CustomIntervals_ms": [], "Height": 1200, "MicroManagerVersion": "1.4.23 20180219", "GridColumn": 0, "UUID": "18808e7c-f2e6-47b3-90d8-1d779ce87dbd", "PixelType": "GRAY16", "Width": 1200, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\Yan\\20191218-YC113-S7S8-miseq", "Frames": 1, "Comment": "", "UserName": "Nikon", "PixelSize_um": 1.1, "ROI": [ 0, 0, 1200, 1200 ], "Date": "2019-12-19", "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC", "P-DAPI-high" ], "KeepShutterOpenSlices": true, "IJType": 1, "ComputerName": "ZadorConfocal2", "Positions": 36, "PositionIndex": 34, "CameraTimeout": 20000, "TimeFirst": false }, "640 AO-Name": "AnalogIO", "Vincent-D3-Command": "Close" }, "FrameKey-0-0-7": { "COM4-StopBits": "1", "528 AO-MaxVolts": "5.0000", "XLIGHT Excitation Wheel-State": "1", "470 Shutter-Name": "AnalogIO", "Core-Shutter": "520", "555 AO-Demo": "No", "XLIGHT Dichroic-State": "2", "XYStage-StepSize-Y": "0.1000", "ZPositionUm": 238.455, "XYStage-StepSize-X": "0.1000", "PRIME-FirmwareVersion": "20.28", "PRIME-ClearCycles": "2", "520-Name": "DA Shutter", "405 Shutter-Name": "AnalogIO", "PIEZO-StepSize": "0.0050", "PRIME-ClearMode": "Pre-Exposure", "470 AO-MinVolts": "0.0000", "405 AO-SupportsTriggering": "1", "CameraChannelIndex": 0, "XYStage-StartSpeed": "500.0000", "COM9-StopBits": "2", "405 AO-Description": "NI DAC", "COM4-DelayBetweenCharsMs": "0.0000", "445 AO-Block voltage": "false", "520 AO-TriggerInputLine": "/Dev1/PFI0", "555 AO-Description": "NI DAC", "520 AO-TriggerSampleRate": "100000", "COM4-Verbose": "1", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM4-Name": "COM4", "405 Shutter-Volts": "0.0000", "Vincent-D3-Port": "COM1", "PRIME-PP 4 MIN ADU AFFECTED": "200", "470 Shutter-MinVolts": "0.0000", "PRIME-PP 3 ENABLED": "Yes", "470 AO-Description": "NI DAC", "PRIME-Description": "PVCAM API device adapter", "520 Shutter-MaxVolts": "5.0000", "520 Shutter-Volts": "5.0000", "528 AO-Sequenceable": "1", "PRIME-Binning": "1x1", "555 Shutter-MaxVolts": "5.0000", "528 Shutter-TriggerSequenceLength": "1000", "405 Shutter-Block voltage": "false", "XLIGHT Spin motor-State": "1", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "Vincent-D3-Time to open (ms)": "35.0000", "Vincent-D3-Time to close (ms)": "35.0000", "445 AO-TriggerSampleRate": "100000", "640 Shutter-IOChannel": "Dev1/ao14", "555 AO-Sequenceable": "1", "520 AO-Name": "AnalogIO", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "470 AO-Block voltage": "false", "COM1-Verbose": "1", "COM9-AnswerTimeout": "10000.0000", "528 Shutter-Name": "AnalogIO", "PRIME-Trigger Timeout (secs)": "10", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "NextFrame": 0, "470 AO-TriggerSequenceLength": "1000", "PRIME-TransposeXY": "0", "445 AO-TriggerSequenceLength": "1000", "COM1-Name": "COM1", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "COM9-DataBits": "8", "COM1-AnswerTimeout": "500.0000", "Camera": "", "TE2000-Version": "V2.50-V2.50-V2.10", "528 Shutter-Volts": "0.0000", "XLIGHT Hub-Name": "XLIGHT Hub", "PIEZO-Description": "Ludl stage driver adapter", "PositionName": "Pos34", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSampleRate": "100000", "PRIME-FanSpeedSetpoint": "High", "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "405 AO-TriggerSampleRate": "100000", "470 Shutter-Block voltage": "false", "470-State": "0", "COM3-Parity": "None", "405 AO-Volts": "0.0000", "PRIME-TransposeMirrorY": "0", "PRIME-ChipName": "GS144BSI", "470 AO-Volts": "0.0000", "PRIME-TransposeMirrorX": "0", "445 Shutter-SupportsTriggering": "0", "470 Shutter-SupportsTriggering": "0", "XLIGHT Hub-Description": "XLIGHT hub", "PRIME-PreampOffLimit": "10000", "470 AO-Sequenceable": "1", "528 Shutter-MaxVolts": "5.0000", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "Core-XYStage": "XYStage", "470 AO-TriggerSampleRate": "100000", "PRIME-Timing-PreTriggerDelayNs": "24672000.0000", "528-DA Device": "528 Shutter", "Focus-Description": "Nikon TE2000 focus adapter", "FilterBlock-State": "5", "520 AO-Demo": "No", "COM3-DelayBetweenCharsMs": "0.0000", "470 Shutter-Volts": "0.0000", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "PRIME-PP 2 THRESHOLD": "125", "PRIME-PP 4 THRESHOLD": "75", "XYStage-Acceleration": "4.0000", "470 Shutter-Demo": "No", "PRIME-Name": "PMPCIECam00", "640-DA Device": "640 Shutter", "405 AO-Block voltage": "false", "ElapsedTime-ms": 1395038, "PRIME-Offset": "100", "445 AO-IOChannel": "Dev1/ao1", "640 AO-MaxVolts": "5.0000", "PositionIndex": 34, "PRIME-PP 1 ENABLED": "Yes", "COM1-BaudRate": "9600", "640-State": "0", "COM3-Verbose": "1", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "LudlController-TransmissionDelay": "0", "PRIME-FullWellCapacity": "62000", "PRIME-SMARTStreamingEnabled": "No", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "640 Shutter-Description": "NI DAC", "528-Description": "DA device that is used as a shutter", "COM4-Description": "Serial port driver (boost:asio)", "405 Shutter-Description": "NI DAC", "TE2000-Name": "TE2000", "528 Shutter-Description": "NI DAC", "COM9-Description": "Serial port driver (boost:asio)", "LudlController-Name": "LudlController", "445 Shutter-TriggerSequenceLength": "1000", "PRIME-SerialNumber": "A18B203008", "445 AO-Volts": "0.0000", "PRIME-X-dimension": "1200", "PRIME-ShutterCloseDelay": "0", "XLIGHT Disk slider-State": "2", "Position": "Default", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 AO-IOChannel": "Dev1/ao5", "520 AO-IOChannel": "Dev1/ao4", "528 AO-TriggerSequenceLength": "1000", "405 Shutter-MaxVolts": "5.0000", "470-DA Device": "470 Shutter", "PRIME-FTCapable": "No", "Core-ChannelGroup": "Channels", "555 AO-SupportsTriggering": "0", "PRIME-Y-dimension": "1200", "Frame": 0, "555 Shutter-Sequenceable": "1", "PRIME-Timing-ExposureTimeNs": "499998000.0000", "Core-ImageProcessor": "", "528-State": "0", "520 AO-Block voltage": "false", "XLIGHT Excitation Wheel-Label": "CFP/YFP", "640 Shutter-Demo": "No", "COM3-StopBits": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "520 Shutter-IOChannel": "Dev1/ao11", "Width": 1200, "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-SupportsTriggering": "0", "640 AO-TriggerSampleRate": "100000", "520-Description": "DA device that is used as a shutter", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "405 Shutter-Sequenceable": "1", "405 Shutter-SupportsTriggering": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-TriggerSampleRate": "100000", "640 Shutter-MaxVolts": "5.0000", "555 AO-MaxVolts": "5.0000", "520 AO-TriggerSequenceLength": "1000", "COM3-BaudRate": "9600", "COM9-BaudRate": "9600", "Vincent-D3-Channel #": "Ch. #1", "PRIME-PMode": "Normal", "OpticalPath-Label": "Left(100)", "BitDepth": 16, "555 AO-TriggerSequenceLength": "1000", "445 Shutter-Description": "NI DAC", "405 AO-Sequenceable": "1", "PRIME-AcquisitionMethod": "Callbacks", "555 AO-Name": "AnalogIO", "PRIME-ShutterMode": "Pre-Exposure", "COM3-Handshaking": "Off", "445 AO-SupportsTriggering": "0", "ROI": "0-0-1200-1200", "COM9-Verbose": "1", "PRIME-Color": "OFF", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "PRIME-ShutterOpenDelay": "0", "COM1-Parity": "None", "Slice": 7, "SlicePosition": 20, "COM9-DelayBetweenCharsMs": "0.0000", "555-Name": "DA Shutter", "PIEZO-LudlSingleAxisName": "B", "XLIGHT Emission Wheel-Label": "565/24(G)", "640 Shutter-Name": "AnalogIO", "640 AO-Sequenceable": "1", "520 Shutter-Demo": "No", "528 Shutter-Demo": "No", "470-Name": "DA Shutter", "520-DA Device": "520 Shutter", "XYStage-Description": "Ludl XY stage driver adapter", "445 Shutter-MinVolts": "0.0000", "528 AO-TriggerSampleRate": "100000", "405 AO-MinVolts": "0.0000", "405 Shutter-TriggerSequenceLength": "1000", "Height": 1200, "555 AO-Block voltage": "false", "640 Shutter-Volts": "0.0000", "PixelType": "GRAY16", "445 Shutter-Block voltage": "false", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "520 Shutter-TriggerSequenceLength": "1000", "COM9-Name": "COM9", "Core-SLM": "", "555 AO-Volts": "0.0000", "555 Shutter-Block voltage": "false", "XYStage-Speed": "1800.0000", "520 AO-MaxVolts": "5.0000", "528 Shutter-SupportsTriggering": "0", "COM1-Description": "Serial port driver (boost:asio)", "PRIME-MetadataEnabled": "No", "520 Shutter-MinVolts": "0.0000", "470 Shutter-Sequenceable": "1", "PRIME-PreampDelay": "0", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-MinVolts": "0.0000", "ChannelIndex": 0, "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Core-AutoShutter": "1", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "Exposure-ms": 320, "PRIME-PP 2 ENABLED": "Yes", "640 Shutter-TriggerSampleRate": "100000", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "470 Shutter-IOChannel": "Dev1/ao10", "405 AO-Demo": "No", "PixelSizeUm": 1.1, "470 AO-Demo": "No", "470 Shutter-MaxVolts": "5.0000", "Vincent-D3-Name": "Vincent-D3", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FileName": "img_000000000_Il-G_007.tif", "LudlController-Reset": "Operate", "PRIME-Exposure": "320.00", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "445-Name": "DA Shutter", "555 Shutter-Name": "AnalogIO", "520 Shutter-TriggerSampleRate": "100000", "445 Shutter-IOChannel": "Dev1/ao9", "PRIME-TransposeCorrection": "0", "XYStage-StepSize": "0.1000", "445-Description": "DA device that is used as a shutter", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 AO-IOChannel": "Dev1/ao7", "520 Shutter-Name": "AnalogIO", "COM3-DataBits": "8", "640 AO-Volts": "0.0000", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "555 Shutter-IOChannel": "Dev1/ao13", "WaitInterval": null, "520 Shutter-Sequenceable": "1", "YPositionUm": 545.6, "445-State": "0", "445-DA Device": "445 Shutter", "PRIME-CircularBufferAutoSize": "ON", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "555 AO-TriggerSampleRate": "100000", "445 Shutter-Volts": "0.0000", "XLIGHT Hub-Port": "COM3", "Core-AutoFocus": "", "COM4-Handshaking": "Off", "528 Shutter-MinVolts": "0.0000", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "445 Shutter-Name": "AnalogIO", "528 Shutter-Sequenceable": "1", "XYStage-TransposeMirrorX": "0", "XYStage-TransposeMirrorY": "0", "PRIME-ReadoutRate": "100MHz 16bit", "XYStage-Name": "Stage", "640 AO-SupportsTriggering": "0", "640-Name": "DA Shutter", "640 Shutter-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "640 AO-Block voltage": "false", "445 Shutter-Sequenceable": "1", "470 AO-MaxVolts": "5.0000", "COM4-BaudRate": "9600", "470-Description": "DA device that is used as a shutter", "520 Shutter-Description": "NI DAC", "PRIME-CCDTemperature": "-14.8500", "Time": "2019-12-19 09:59:25 -0500", "640 AO-TriggerSequenceLength": "1000", "470 AO-Name": "AnalogIO", "XLIGHT Disk slider-Label": "Disk Pos 40um", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "Binning": "1x1", "TE2000-ModelType": "1", "COM3-AnswerTimeout": "500.0000", "555 Shutter-SupportsTriggering": "0", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "TE2000-Port": "COM4", "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Description": "NI DAC", "COM4-AnswerTimeout": "500.0000", "COM1-DelayBetweenCharsMs": "0.0000", "FilterBlock-Label": "Confocal", "445 AO-Name": "AnalogIO", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-Timing-ReadoutTimeNs": "24672000.0000", "528 AO-Demo": "No", "520 AO-Sequenceable": "1", "555-Description": "DA device that is used as a shutter", "405-DA Device": "405 Shutter", "405 AO-TriggerSequenceLength": "1000", "640 AO-MinVolts": "0.0000", "640 Shutter-Block voltage": "false", "FrameIndex": 0, "PRIME-Port": "CMOS", "555 AO-MinVolts": "0.0000", "COM3-Description": "Serial port driver (boost:asio)", "528 AO-Description": "NI DAC", "555 AO-IOChannel": "Dev1/ao6", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "555 AO-TriggerInputLine": "/Dev1/PFI0", "PRIME-ActualInterval-ms": "390.5200", "LudlController-Port": "COM9", "445 Shutter-Demo": "No", "PRIME-Trigger-Expose Out-Mux": "1", "SliceIndex": 7, "PRIME-CCDTemperatureSetPoint": "-15.0000", "405 Shutter-MinVolts": "0.0000", "PRIME-ExposeOutMode": "First Row", "AxisPositions": null, "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Volts": "0.0000", "528-Name": "DA Shutter", "555-State": "0", "XLIGHT Dichroic-Label": "CFP/YFP", "445 AO-Sequenceable": "1", "405-Name": "DA Shutter", "405 AO-IOChannel": "Dev1/ao0", "Core-Camera": "PRIME", "Focus-Name": "Focus", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "405 Shutter-IOChannel": "Dev1/ao8", "640 AO-Demo": "No", "640 Shutter-SupportsTriggering": "0", "445 AO-Demo": "No", "555-DA Device": "555 Shutter", "470 Shutter-Description": "NI DAC", "528 AO-Volts": "0.0000", "COM1-DataBits": "8", "Vincent-D3-Address": "x", "520 AO-SupportsTriggering": "0", "405-Description": "DA device that is used as a shutter", "640 Shutter-MinVolts": "0.0000", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "405 AO-Name": "AnalogIO", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "640 AO-TriggerInputLine": "/Dev1/PFI0", "405 AO-TriggerInputLine": "/Dev1/PFI0", "640-Description": "DA device that is used as a shutter", "445 Shutter-TriggerSampleRate": "100000", "470 Shutter-TriggerSequenceLength": "1000", "COM1-StopBits": "1", "520 Shutter-SupportsTriggering": "0", "405-State": "0", "Core-Initialize": "1", "XLIGHT Emission Wheel-State": "3", "520 AO-Description": "NI DAC", "528 AO-TriggerInputLine": "/Dev1/PFI0", "445 Shutter-MaxVolts": "5.0000", "COM9-Handshaking": "Off", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "XLIGHT Spin motor-Label": "On (spinning)", "PRIME-PP 5 ENABLED": "No", "UUID": "29dda5a0-b22e-4b54-9ed6-1fbcab07acfb", "PRIME-TriggerMode": "Internal Trigger", "COM1-Handshaking": "Off", "PRIME-Timing-PostTriggerDelayNs": "49344000.0000", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "Focus-Position": "17741.5000", "528 Shutter-Block voltage": "false", "Channel": "Il-G", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "555 Shutter-Demo": "No", "405 Shutter-Demo": "No", "528 AO-Name": "AnalogIO", "520-State": "1", "Core-Galvo": "", "405 Shutter-TriggerSampleRate": "100000", "Focus-FocusStepSize_(nm)": "100", "PRIME-PixelType": "16bit", "COM4-DataBits": "8", "470 AO-IOChannel": "Dev1/ao2", "520 AO-MinVolts": "0.0000", "COM9-Parity": "None", "PRIME-CircularBufferEnabled": "ON", "PRIME-PP 6 Reset": "No", "640 AO-Description": "NI DAC", "Source": "PRIME", "445 AO-MinVolts": "0.0000", "PIEZO-Name": "Stage", "445 AO-Description": "NI DAC", "PRIME-CircularBufferFrameCount": "46", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "520 AO-Volts": "4.5000", "528 Shutter-TriggerSampleRate": "100000", "XPositionUm": -1324.9, "OpticalPath-State": "4", "PRIME-PP 3 THRESHOLD": "80", "PRIME-PVCAM Version": "3.7.1", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Gain": "1-HDR", "640 Shutter-TriggerSequenceLength": "1000", "528 AO-SupportsTriggering": "0", "445 AO-TriggerInputLine": "/Dev1/PFI0", "OpticalPath-Name": "OpticalPath", "PRIME-PP 4 ENABLED": "Yes", "PRIME-PVCAM Adapter Version": "1.3.29", "COM3-Name": "COM3", "405 AO-MaxVolts": "5.0000", "520 Shutter-Block voltage": "false", "PRIME-Timing-ClearingTimeNs": "49344000.0000", "Core-Focus": "PIEZO", "528 AO-Block voltage": "false", "TE2000-Description": "Nikon TE2000 hub", "528 AO-MinVolts": "0.0000", "COM4-Parity": "None", "Summary": { "Slices": 11, "ChContrastMin": [ 0, 0, 0, 0, 0, 0 ], "PixelAspect": 1, "SlicesFirst": true, "Interval_ms": 0, "Time": "2019-12-19 09:59:21 -0500", "Prefix": "seq_13", "InitialPositionList": [ { "Label": "Pos0", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.21], "Focus": [17741.5], "XYStage": [ 44542.200000000004, 31636.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos1", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.22], "Focus": [17741.4], "XYStage": [ 43801.100000000006, 31637.9 ] }, "GridColumnIndex": 0 }, { "Label": "Pos2", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.5], "XYStage": [ 38561.3, 32040.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos3", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.185], "Focus": [17741.3], "XYStage": [ 37698.4, 32041.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos4", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.16], "Focus": [17741.5], "XYStage": [ 32375.4, 31758.9 ] }, "GridColumnIndex": 0 }, { "Label": "Pos5", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.17000000000002], "Focus": [17741.3], "XYStage": [ 31770.7, 31758.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos6", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.4], "XYStage": [ 26536.800000000003, 31068.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos7", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.205], "Focus": [17741.4], "XYStage": [ 25420.2, 31068.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos8", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.85], "Focus": [17741.3], "XYStage": [ 21059, 31508 ] }, "GridColumnIndex": 0 }, { "Label": "Pos9", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.82], "Focus": [17741.3], "XYStage": [ 19731.9, 31506.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos10", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.79], "Focus": [17741.4], "XYStage": [ 14528.5, 32007.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos11", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.83], "Focus": [17741.2], "XYStage": [ 13830.300000000001, 32009.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos12", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.79], "Focus": [17741.3], "XYStage": [ 8775.300000000001, 32017.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos13", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.815], "Focus": [17741.3], "XYStage": [ 8109.200000000001, 32008.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos14", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.805], "Focus": [17741.3], "XYStage": [ 3483.3, 31908.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos15", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.82], "Focus": [17741.2], "XYStage": [ 2686.8, 31903.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos16", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [211.48000000000002], "Focus": [17741.1], "XYStage": [ -1978.9, 31973.600000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos17", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [211.51500000000001], "Focus": [17741.1], "XYStage": [ -2957.8, 31982.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos18", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.195], "Focus": [17741.5], "XYStage": [ 47747.4, -576.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos19", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.23000000000002], "Focus": [17741.6], "XYStage": [ 47053.9, -576.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos20", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.205], "Focus": [17741.4], "XYStage": [ 41871.200000000004, -479.8 ] }, "GridColumnIndex": 0 }, { "Label": "Pos21", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.4], "XYStage": [ 40882.600000000006, -392 ] }, "GridColumnIndex": 0 }, { "Label": "Pos22", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.24], "Focus": [17741.4], "XYStage": [ 35905.6, -135.3 ] }, "GridColumnIndex": 0 }, { "Label": "Pos23", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.195], "Focus": [17741.5], "XYStage": [ 34990.9, -132.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos24", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.46], "Focus": [17741.4], "XYStage": [ 29678.100000000002, -958.1 ] }, "GridColumnIndex": 0 }, { "Label": "Pos25", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.44], "Focus": [17741.5], "XYStage": [ 28983.600000000002, -939.4000000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos26", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.475], "Focus": [17741.5], "XYStage": [ 23019.7, -122 ] }, "GridColumnIndex": 0 }, { "Label": "Pos27", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.44], "Focus": [17741.7], "XYStage": [ 22428, -116.80000000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos28", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.46], "Focus": [17741.7], "XYStage": [ 16364.900000000001, -181.10000000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos29", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.48000000000002], "Focus": [17741.5], "XYStage": [ 15712.1, -180 ] }, "GridColumnIndex": 0 }, { "Label": "Pos30", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.45000000000002], "Focus": [17741.7], "XYStage": [ 10346.1, 77.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos31", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.49], "Focus": [17741.4], "XYStage": [ 9755.5, 85.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos32", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.475], "Focus": [17741.5], "XYStage": [ 4775.5, 293.90000000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos33", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.445], "Focus": [17741.4], "XYStage": [ 3954.9, 293.90000000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos34", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.455], "Focus": [17741.4], "XYStage": [ -1324.9, 545.6 ] }, "GridColumnIndex": 0 }, { "Label": "Pos35", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.445], "Focus": [17741.3], "XYStage": [ -2097, 576.6 ] }, "GridColumnIndex": 0 } ], "z-step_um": 10, "GridRow": 0, "BitDepth": 16, "Depth": 2, "Channels": 6, "Source": "Micro-Manager", "ChColors": [ -16711681, -256, -65281, -1, -1, -16776961 ], "MetadataVersion": 10, "KeepShutterOpenChannels": true, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536, 65536 ], "CustomIntervals_ms": [], "Height": 1200, "MicroManagerVersion": "1.4.23 20180219", "GridColumn": 0, "UUID": "18808e7c-f2e6-47b3-90d8-1d779ce87dbd", "PixelType": "GRAY16", "Width": 1200, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\Yan\\20191218-YC113-S7S8-miseq", "Frames": 1, "Comment": "", "UserName": "Nikon", "PixelSize_um": 1.1, "ROI": [ 0, 0, 1200, 1200 ], "Date": "2019-12-19", "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC", "P-DAPI-high" ], "KeepShutterOpenSlices": true, "IJType": 1, "ComputerName": "ZadorConfocal2", "Positions": 36, "PositionIndex": 34, "CameraTimeout": 20000, "TimeFirst": false }, "640 AO-Name": "AnalogIO", "Vincent-D3-Command": "Close" }, "FrameKey-0-0-8": { "COM4-StopBits": "1", "528 AO-MaxVolts": "5.0000", "XLIGHT Excitation Wheel-State": "1", "470 Shutter-Name": "AnalogIO", "Core-Shutter": "520", "555 AO-Demo": "No", "XLIGHT Dichroic-State": "2", "XYStage-StepSize-Y": "0.1000", "ZPositionUm": 248.455, "XYStage-StepSize-X": "0.1000", "PRIME-FirmwareVersion": "20.28", "PRIME-ClearCycles": "2", "520-Name": "DA Shutter", "405 Shutter-Name": "AnalogIO", "PIEZO-StepSize": "0.0050", "PRIME-ClearMode": "Pre-Exposure", "470 AO-MinVolts": "0.0000", "405 AO-SupportsTriggering": "1", "CameraChannelIndex": 0, "XYStage-StartSpeed": "500.0000", "COM9-StopBits": "2", "405 AO-Description": "NI DAC", "COM4-DelayBetweenCharsMs": "0.0000", "445 AO-Block voltage": "false", "520 AO-TriggerInputLine": "/Dev1/PFI0", "555 AO-Description": "NI DAC", "520 AO-TriggerSampleRate": "100000", "COM4-Verbose": "1", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM4-Name": "COM4", "405 Shutter-Volts": "0.0000", "Vincent-D3-Port": "COM1", "PRIME-PP 4 MIN ADU AFFECTED": "200", "470 Shutter-MinVolts": "0.0000", "PRIME-PP 3 ENABLED": "Yes", "470 AO-Description": "NI DAC", "PRIME-Description": "PVCAM API device adapter", "520 Shutter-MaxVolts": "5.0000", "520 Shutter-Volts": "5.0000", "528 AO-Sequenceable": "1", "PRIME-Binning": "1x1", "555 Shutter-MaxVolts": "5.0000", "528 Shutter-TriggerSequenceLength": "1000", "405 Shutter-Block voltage": "false", "XLIGHT Spin motor-State": "1", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "Vincent-D3-Time to open (ms)": "35.0000", "Vincent-D3-Time to close (ms)": "35.0000", "445 AO-TriggerSampleRate": "100000", "640 Shutter-IOChannel": "Dev1/ao14", "555 AO-Sequenceable": "1", "520 AO-Name": "AnalogIO", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "470 AO-Block voltage": "false", "COM1-Verbose": "1", "COM9-AnswerTimeout": "10000.0000", "528 Shutter-Name": "AnalogIO", "PRIME-Trigger Timeout (secs)": "10", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "NextFrame": 0, "470 AO-TriggerSequenceLength": "1000", "PRIME-TransposeXY": "0", "445 AO-TriggerSequenceLength": "1000", "COM1-Name": "COM1", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "COM9-DataBits": "8", "COM1-AnswerTimeout": "500.0000", "Camera": "", "TE2000-Version": "V2.50-V2.50-V2.10", "528 Shutter-Volts": "0.0000", "XLIGHT Hub-Name": "XLIGHT Hub", "PIEZO-Description": "Ludl stage driver adapter", "PositionName": "Pos34", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSampleRate": "100000", "PRIME-FanSpeedSetpoint": "High", "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "405 AO-TriggerSampleRate": "100000", "470 Shutter-Block voltage": "false", "470-State": "0", "COM3-Parity": "None", "405 AO-Volts": "0.0000", "PRIME-TransposeMirrorY": "0", "PRIME-ChipName": "GS144BSI", "470 AO-Volts": "0.0000", "PRIME-TransposeMirrorX": "0", "445 Shutter-SupportsTriggering": "0", "470 Shutter-SupportsTriggering": "0", "XLIGHT Hub-Description": "XLIGHT hub", "PRIME-PreampOffLimit": "10000", "470 AO-Sequenceable": "1", "528 Shutter-MaxVolts": "5.0000", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "Core-XYStage": "XYStage", "470 AO-TriggerSampleRate": "100000", "PRIME-Timing-PreTriggerDelayNs": "24672000.0000", "528-DA Device": "528 Shutter", "Focus-Description": "Nikon TE2000 focus adapter", "FilterBlock-State": "5", "520 AO-Demo": "No", "COM3-DelayBetweenCharsMs": "0.0000", "470 Shutter-Volts": "0.0000", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "PRIME-PP 2 THRESHOLD": "125", "PRIME-PP 4 THRESHOLD": "75", "XYStage-Acceleration": "4.0000", "470 Shutter-Demo": "No", "PRIME-Name": "PMPCIECam00", "640-DA Device": "640 Shutter", "405 AO-Block voltage": "false", "ElapsedTime-ms": 1395594, "PRIME-Offset": "100", "445 AO-IOChannel": "Dev1/ao1", "640 AO-MaxVolts": "5.0000", "PositionIndex": 34, "PRIME-PP 1 ENABLED": "Yes", "COM1-BaudRate": "9600", "640-State": "0", "COM3-Verbose": "1", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "LudlController-TransmissionDelay": "0", "PRIME-FullWellCapacity": "62000", "PRIME-SMARTStreamingEnabled": "No", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "640 Shutter-Description": "NI DAC", "528-Description": "DA device that is used as a shutter", "COM4-Description": "Serial port driver (boost:asio)", "405 Shutter-Description": "NI DAC", "TE2000-Name": "TE2000", "528 Shutter-Description": "NI DAC", "COM9-Description": "Serial port driver (boost:asio)", "LudlController-Name": "LudlController", "445 Shutter-TriggerSequenceLength": "1000", "PRIME-SerialNumber": "A18B203008", "445 AO-Volts": "0.0000", "PRIME-X-dimension": "1200", "PRIME-ShutterCloseDelay": "0", "XLIGHT Disk slider-State": "2", "Position": "Default", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 AO-IOChannel": "Dev1/ao5", "520 AO-IOChannel": "Dev1/ao4", "528 AO-TriggerSequenceLength": "1000", "405 Shutter-MaxVolts": "5.0000", "470-DA Device": "470 Shutter", "PRIME-FTCapable": "No", "Core-ChannelGroup": "Channels", "555 AO-SupportsTriggering": "0", "PRIME-Y-dimension": "1200", "Frame": 0, "555 Shutter-Sequenceable": "1", "PRIME-Timing-ExposureTimeNs": "499998000.0000", "Core-ImageProcessor": "", "528-State": "0", "520 AO-Block voltage": "false", "XLIGHT Excitation Wheel-Label": "CFP/YFP", "640 Shutter-Demo": "No", "COM3-StopBits": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "520 Shutter-IOChannel": "Dev1/ao11", "Width": 1200, "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-SupportsTriggering": "0", "640 AO-TriggerSampleRate": "100000", "520-Description": "DA device that is used as a shutter", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "405 Shutter-Sequenceable": "1", "405 Shutter-SupportsTriggering": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-TriggerSampleRate": "100000", "640 Shutter-MaxVolts": "5.0000", "555 AO-MaxVolts": "5.0000", "520 AO-TriggerSequenceLength": "1000", "COM3-BaudRate": "9600", "COM9-BaudRate": "9600", "Vincent-D3-Channel #": "Ch. #1", "PRIME-PMode": "Normal", "OpticalPath-Label": "Left(100)", "BitDepth": 16, "555 AO-TriggerSequenceLength": "1000", "445 Shutter-Description": "NI DAC", "405 AO-Sequenceable": "1", "PRIME-AcquisitionMethod": "Callbacks", "555 AO-Name": "AnalogIO", "PRIME-ShutterMode": "Pre-Exposure", "COM3-Handshaking": "Off", "445 AO-SupportsTriggering": "0", "ROI": "0-0-1200-1200", "COM9-Verbose": "1", "PRIME-Color": "OFF", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "PRIME-ShutterOpenDelay": "0", "COM1-Parity": "None", "Slice": 8, "SlicePosition": 30, "COM9-DelayBetweenCharsMs": "0.0000", "555-Name": "DA Shutter", "PIEZO-LudlSingleAxisName": "B", "XLIGHT Emission Wheel-Label": "565/24(G)", "640 Shutter-Name": "AnalogIO", "640 AO-Sequenceable": "1", "520 Shutter-Demo": "No", "528 Shutter-Demo": "No", "470-Name": "DA Shutter", "520-DA Device": "520 Shutter", "XYStage-Description": "Ludl XY stage driver adapter", "445 Shutter-MinVolts": "0.0000", "528 AO-TriggerSampleRate": "100000", "405 AO-MinVolts": "0.0000", "405 Shutter-TriggerSequenceLength": "1000", "Height": 1200, "555 AO-Block voltage": "false", "640 Shutter-Volts": "0.0000", "PixelType": "GRAY16", "445 Shutter-Block voltage": "false", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "520 Shutter-TriggerSequenceLength": "1000", "COM9-Name": "COM9", "Core-SLM": "", "555 AO-Volts": "0.0000", "555 Shutter-Block voltage": "false", "XYStage-Speed": "1800.0000", "520 AO-MaxVolts": "5.0000", "528 Shutter-SupportsTriggering": "0", "COM1-Description": "Serial port driver (boost:asio)", "PRIME-MetadataEnabled": "No", "520 Shutter-MinVolts": "0.0000", "470 Shutter-Sequenceable": "1", "PRIME-PreampDelay": "0", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-MinVolts": "0.0000", "ChannelIndex": 0, "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Core-AutoShutter": "1", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "Exposure-ms": 320, "PRIME-PP 2 ENABLED": "Yes", "640 Shutter-TriggerSampleRate": "100000", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "470 Shutter-IOChannel": "Dev1/ao10", "405 AO-Demo": "No", "PixelSizeUm": 1.1, "470 AO-Demo": "No", "470 Shutter-MaxVolts": "5.0000", "Vincent-D3-Name": "Vincent-D3", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FileName": "img_000000000_Il-G_008.tif", "LudlController-Reset": "Operate", "PRIME-Exposure": "320.00", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "445-Name": "DA Shutter", "555 Shutter-Name": "AnalogIO", "520 Shutter-TriggerSampleRate": "100000", "445 Shutter-IOChannel": "Dev1/ao9", "PRIME-TransposeCorrection": "0", "XYStage-StepSize": "0.1000", "445-Description": "DA device that is used as a shutter", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 AO-IOChannel": "Dev1/ao7", "520 Shutter-Name": "AnalogIO", "COM3-DataBits": "8", "640 AO-Volts": "0.0000", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "555 Shutter-IOChannel": "Dev1/ao13", "WaitInterval": null, "520 Shutter-Sequenceable": "1", "YPositionUm": 545.6, "445-State": "0", "445-DA Device": "445 Shutter", "PRIME-CircularBufferAutoSize": "ON", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "555 AO-TriggerSampleRate": "100000", "445 Shutter-Volts": "0.0000", "XLIGHT Hub-Port": "COM3", "Core-AutoFocus": "", "COM4-Handshaking": "Off", "528 Shutter-MinVolts": "0.0000", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "445 Shutter-Name": "AnalogIO", "528 Shutter-Sequenceable": "1", "XYStage-TransposeMirrorX": "0", "XYStage-TransposeMirrorY": "0", "PRIME-ReadoutRate": "100MHz 16bit", "XYStage-Name": "Stage", "640 AO-SupportsTriggering": "0", "640-Name": "DA Shutter", "640 Shutter-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "640 AO-Block voltage": "false", "445 Shutter-Sequenceable": "1", "470 AO-MaxVolts": "5.0000", "COM4-BaudRate": "9600", "470-Description": "DA device that is used as a shutter", "520 Shutter-Description": "NI DAC", "PRIME-CCDTemperature": "-14.8500", "Time": "2019-12-19 09:59:25 -0500", "640 AO-TriggerSequenceLength": "1000", "470 AO-Name": "AnalogIO", "XLIGHT Disk slider-Label": "Disk Pos 40um", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "Binning": "1x1", "TE2000-ModelType": "1", "COM3-AnswerTimeout": "500.0000", "555 Shutter-SupportsTriggering": "0", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "TE2000-Port": "COM4", "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Description": "NI DAC", "COM4-AnswerTimeout": "500.0000", "COM1-DelayBetweenCharsMs": "0.0000", "FilterBlock-Label": "Confocal", "445 AO-Name": "AnalogIO", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-Timing-ReadoutTimeNs": "24672000.0000", "528 AO-Demo": "No", "520 AO-Sequenceable": "1", "555-Description": "DA device that is used as a shutter", "405-DA Device": "405 Shutter", "405 AO-TriggerSequenceLength": "1000", "640 AO-MinVolts": "0.0000", "640 Shutter-Block voltage": "false", "FrameIndex": 0, "PRIME-Port": "CMOS", "555 AO-MinVolts": "0.0000", "COM3-Description": "Serial port driver (boost:asio)", "528 AO-Description": "NI DAC", "555 AO-IOChannel": "Dev1/ao6", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "555 AO-TriggerInputLine": "/Dev1/PFI0", "PRIME-ActualInterval-ms": "390.5200", "LudlController-Port": "COM9", "445 Shutter-Demo": "No", "PRIME-Trigger-Expose Out-Mux": "1", "SliceIndex": 8, "PRIME-CCDTemperatureSetPoint": "-15.0000", "405 Shutter-MinVolts": "0.0000", "PRIME-ExposeOutMode": "First Row", "AxisPositions": null, "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Volts": "0.0000", "528-Name": "DA Shutter", "555-State": "0", "XLIGHT Dichroic-Label": "CFP/YFP", "445 AO-Sequenceable": "1", "405-Name": "DA Shutter", "405 AO-IOChannel": "Dev1/ao0", "Core-Camera": "PRIME", "Focus-Name": "Focus", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "405 Shutter-IOChannel": "Dev1/ao8", "640 AO-Demo": "No", "640 Shutter-SupportsTriggering": "0", "445 AO-Demo": "No", "555-DA Device": "555 Shutter", "470 Shutter-Description": "NI DAC", "528 AO-Volts": "0.0000", "COM1-DataBits": "8", "Vincent-D3-Address": "x", "520 AO-SupportsTriggering": "0", "405-Description": "DA device that is used as a shutter", "640 Shutter-MinVolts": "0.0000", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "405 AO-Name": "AnalogIO", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "640 AO-TriggerInputLine": "/Dev1/PFI0", "405 AO-TriggerInputLine": "/Dev1/PFI0", "640-Description": "DA device that is used as a shutter", "445 Shutter-TriggerSampleRate": "100000", "470 Shutter-TriggerSequenceLength": "1000", "COM1-StopBits": "1", "520 Shutter-SupportsTriggering": "0", "405-State": "0", "Core-Initialize": "1", "XLIGHT Emission Wheel-State": "3", "520 AO-Description": "NI DAC", "528 AO-TriggerInputLine": "/Dev1/PFI0", "445 Shutter-MaxVolts": "5.0000", "COM9-Handshaking": "Off", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "XLIGHT Spin motor-Label": "On (spinning)", "PRIME-PP 5 ENABLED": "No", "UUID": "d9a07a8b-08e1-4517-919f-438da88fa029", "PRIME-TriggerMode": "Internal Trigger", "COM1-Handshaking": "Off", "PRIME-Timing-PostTriggerDelayNs": "49344000.0000", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "Focus-Position": "17741.5000", "528 Shutter-Block voltage": "false", "Channel": "Il-G", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "555 Shutter-Demo": "No", "405 Shutter-Demo": "No", "528 AO-Name": "AnalogIO", "520-State": "1", "Core-Galvo": "", "405 Shutter-TriggerSampleRate": "100000", "Focus-FocusStepSize_(nm)": "100", "PRIME-PixelType": "16bit", "COM4-DataBits": "8", "470 AO-IOChannel": "Dev1/ao2", "520 AO-MinVolts": "0.0000", "COM9-Parity": "None", "PRIME-CircularBufferEnabled": "ON", "PRIME-PP 6 Reset": "No", "640 AO-Description": "NI DAC", "Source": "PRIME", "445 AO-MinVolts": "0.0000", "PIEZO-Name": "Stage", "445 AO-Description": "NI DAC", "PRIME-CircularBufferFrameCount": "46", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "520 AO-Volts": "4.5000", "528 Shutter-TriggerSampleRate": "100000", "XPositionUm": -1324.9, "OpticalPath-State": "4", "PRIME-PP 3 THRESHOLD": "80", "PRIME-PVCAM Version": "3.7.1", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Gain": "1-HDR", "640 Shutter-TriggerSequenceLength": "1000", "528 AO-SupportsTriggering": "0", "445 AO-TriggerInputLine": "/Dev1/PFI0", "OpticalPath-Name": "OpticalPath", "PRIME-PP 4 ENABLED": "Yes", "PRIME-PVCAM Adapter Version": "1.3.29", "COM3-Name": "COM3", "405 AO-MaxVolts": "5.0000", "520 Shutter-Block voltage": "false", "PRIME-Timing-ClearingTimeNs": "49344000.0000", "Core-Focus": "PIEZO", "528 AO-Block voltage": "false", "TE2000-Description": "Nikon TE2000 hub", "528 AO-MinVolts": "0.0000", "COM4-Parity": "None", "Summary": { "Slices": 11, "ChContrastMin": [ 0, 0, 0, 0, 0, 0 ], "PixelAspect": 1, "SlicesFirst": true, "Interval_ms": 0, "Time": "2019-12-19 09:59:21 -0500", "Prefix": "seq_13", "InitialPositionList": [ { "Label": "Pos0", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.21], "Focus": [17741.5], "XYStage": [ 44542.200000000004, 31636.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos1", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.22], "Focus": [17741.4], "XYStage": [ 43801.100000000006, 31637.9 ] }, "GridColumnIndex": 0 }, { "Label": "Pos2", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.5], "XYStage": [ 38561.3, 32040.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos3", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.185], "Focus": [17741.3], "XYStage": [ 37698.4, 32041.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos4", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.16], "Focus": [17741.5], "XYStage": [ 32375.4, 31758.9 ] }, "GridColumnIndex": 0 }, { "Label": "Pos5", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.17000000000002], "Focus": [17741.3], "XYStage": [ 31770.7, 31758.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos6", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.4], "XYStage": [ 26536.800000000003, 31068.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos7", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.205], "Focus": [17741.4], "XYStage": [ 25420.2, 31068.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos8", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.85], "Focus": [17741.3], "XYStage": [ 21059, 31508 ] }, "GridColumnIndex": 0 }, { "Label": "Pos9", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.82], "Focus": [17741.3], "XYStage": [ 19731.9, 31506.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos10", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.79], "Focus": [17741.4], "XYStage": [ 14528.5, 32007.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos11", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.83], "Focus": [17741.2], "XYStage": [ 13830.300000000001, 32009.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos12", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.79], "Focus": [17741.3], "XYStage": [ 8775.300000000001, 32017.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos13", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.815], "Focus": [17741.3], "XYStage": [ 8109.200000000001, 32008.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos14", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.805], "Focus": [17741.3], "XYStage": [ 3483.3, 31908.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos15", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.82], "Focus": [17741.2], "XYStage": [ 2686.8, 31903.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos16", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [211.48000000000002], "Focus": [17741.1], "XYStage": [ -1978.9, 31973.600000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos17", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [211.51500000000001], "Focus": [17741.1], "XYStage": [ -2957.8, 31982.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos18", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.195], "Focus": [17741.5], "XYStage": [ 47747.4, -576.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos19", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.23000000000002], "Focus": [17741.6], "XYStage": [ 47053.9, -576.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos20", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.205], "Focus": [17741.4], "XYStage": [ 41871.200000000004, -479.8 ] }, "GridColumnIndex": 0 }, { "Label": "Pos21", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.4], "XYStage": [ 40882.600000000006, -392 ] }, "GridColumnIndex": 0 }, { "Label": "Pos22", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.24], "Focus": [17741.4], "XYStage": [ 35905.6, -135.3 ] }, "GridColumnIndex": 0 }, { "Label": "Pos23", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.195], "Focus": [17741.5], "XYStage": [ 34990.9, -132.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos24", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.46], "Focus": [17741.4], "XYStage": [ 29678.100000000002, -958.1 ] }, "GridColumnIndex": 0 }, { "Label": "Pos25", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.44], "Focus": [17741.5], "XYStage": [ 28983.600000000002, -939.4000000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos26", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.475], "Focus": [17741.5], "XYStage": [ 23019.7, -122 ] }, "GridColumnIndex": 0 }, { "Label": "Pos27", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.44], "Focus": [17741.7], "XYStage": [ 22428, -116.80000000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos28", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.46], "Focus": [17741.7], "XYStage": [ 16364.900000000001, -181.10000000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos29", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.48000000000002], "Focus": [17741.5], "XYStage": [ 15712.1, -180 ] }, "GridColumnIndex": 0 }, { "Label": "Pos30", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.45000000000002], "Focus": [17741.7], "XYStage": [ 10346.1, 77.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos31", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.49], "Focus": [17741.4], "XYStage": [ 9755.5, 85.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos32", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.475], "Focus": [17741.5], "XYStage": [ 4775.5, 293.90000000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos33", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.445], "Focus": [17741.4], "XYStage": [ 3954.9, 293.90000000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos34", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.455], "Focus": [17741.4], "XYStage": [ -1324.9, 545.6 ] }, "GridColumnIndex": 0 }, { "Label": "Pos35", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.445], "Focus": [17741.3], "XYStage": [ -2097, 576.6 ] }, "GridColumnIndex": 0 } ], "z-step_um": 10, "GridRow": 0, "BitDepth": 16, "Depth": 2, "Channels": 6, "Source": "Micro-Manager", "ChColors": [ -16711681, -256, -65281, -1, -1, -16776961 ], "MetadataVersion": 10, "KeepShutterOpenChannels": true, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536, 65536 ], "CustomIntervals_ms": [], "Height": 1200, "MicroManagerVersion": "1.4.23 20180219", "GridColumn": 0, "UUID": "18808e7c-f2e6-47b3-90d8-1d779ce87dbd", "PixelType": "GRAY16", "Width": 1200, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\Yan\\20191218-YC113-S7S8-miseq", "Frames": 1, "Comment": "", "UserName": "Nikon", "PixelSize_um": 1.1, "ROI": [ 0, 0, 1200, 1200 ], "Date": "2019-12-19", "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC", "P-DAPI-high" ], "KeepShutterOpenSlices": true, "IJType": 1, "ComputerName": "ZadorConfocal2", "Positions": 36, "PositionIndex": 34, "CameraTimeout": 20000, "TimeFirst": false }, "640 AO-Name": "AnalogIO", "Vincent-D3-Command": "Close" }, "FrameKey-0-0-9": { "COM4-StopBits": "1", "528 AO-MaxVolts": "5.0000", "XLIGHT Excitation Wheel-State": "1", "470 Shutter-Name": "AnalogIO", "Core-Shutter": "520", "555 AO-Demo": "No", "XLIGHT Dichroic-State": "2", "XYStage-StepSize-Y": "0.1000", "ZPositionUm": 258.45500000000004, "XYStage-StepSize-X": "0.1000", "PRIME-FirmwareVersion": "20.28", "PRIME-ClearCycles": "2", "520-Name": "DA Shutter", "405 Shutter-Name": "AnalogIO", "PIEZO-StepSize": "0.0050", "PRIME-ClearMode": "Pre-Exposure", "470 AO-MinVolts": "0.0000", "405 AO-SupportsTriggering": "1", "CameraChannelIndex": 0, "XYStage-StartSpeed": "500.0000", "COM9-StopBits": "2", "405 AO-Description": "NI DAC", "COM4-DelayBetweenCharsMs": "0.0000", "445 AO-Block voltage": "false", "520 AO-TriggerInputLine": "/Dev1/PFI0", "555 AO-Description": "NI DAC", "520 AO-TriggerSampleRate": "100000", "COM4-Verbose": "1", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM4-Name": "COM4", "405 Shutter-Volts": "0.0000", "Vincent-D3-Port": "COM1", "PRIME-PP 4 MIN ADU AFFECTED": "200", "470 Shutter-MinVolts": "0.0000", "PRIME-PP 3 ENABLED": "Yes", "470 AO-Description": "NI DAC", "PRIME-Description": "PVCAM API device adapter", "520 Shutter-MaxVolts": "5.0000", "520 Shutter-Volts": "5.0000", "528 AO-Sequenceable": "1", "PRIME-Binning": "1x1", "555 Shutter-MaxVolts": "5.0000", "528 Shutter-TriggerSequenceLength": "1000", "405 Shutter-Block voltage": "false", "XLIGHT Spin motor-State": "1", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "Vincent-D3-Time to open (ms)": "35.0000", "Vincent-D3-Time to close (ms)": "35.0000", "445 AO-TriggerSampleRate": "100000", "640 Shutter-IOChannel": "Dev1/ao14", "555 AO-Sequenceable": "1", "520 AO-Name": "AnalogIO", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "470 AO-Block voltage": "false", "COM1-Verbose": "1", "COM9-AnswerTimeout": "10000.0000", "528 Shutter-Name": "AnalogIO", "PRIME-Trigger Timeout (secs)": "10", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "NextFrame": 0, "470 AO-TriggerSequenceLength": "1000", "PRIME-TransposeXY": "0", "445 AO-TriggerSequenceLength": "1000", "COM1-Name": "COM1", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "COM9-DataBits": "8", "COM1-AnswerTimeout": "500.0000", "Camera": "", "TE2000-Version": "V2.50-V2.50-V2.10", "528 Shutter-Volts": "0.0000", "XLIGHT Hub-Name": "XLIGHT Hub", "PIEZO-Description": "Ludl stage driver adapter", "PositionName": "Pos34", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSampleRate": "100000", "PRIME-FanSpeedSetpoint": "High", "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "405 AO-TriggerSampleRate": "100000", "470 Shutter-Block voltage": "false", "470-State": "0", "COM3-Parity": "None", "405 AO-Volts": "0.0000", "PRIME-TransposeMirrorY": "0", "PRIME-ChipName": "GS144BSI", "470 AO-Volts": "0.0000", "PRIME-TransposeMirrorX": "0", "445 Shutter-SupportsTriggering": "0", "470 Shutter-SupportsTriggering": "0", "XLIGHT Hub-Description": "XLIGHT hub", "PRIME-PreampOffLimit": "10000", "470 AO-Sequenceable": "1", "528 Shutter-MaxVolts": "5.0000", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "Core-XYStage": "XYStage", "470 AO-TriggerSampleRate": "100000", "PRIME-Timing-PreTriggerDelayNs": "24672000.0000", "528-DA Device": "528 Shutter", "Focus-Description": "Nikon TE2000 focus adapter", "FilterBlock-State": "5", "520 AO-Demo": "No", "COM3-DelayBetweenCharsMs": "0.0000", "470 Shutter-Volts": "0.0000", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "PRIME-PP 2 THRESHOLD": "125", "PRIME-PP 4 THRESHOLD": "75", "XYStage-Acceleration": "4.0000", "470 Shutter-Demo": "No", "PRIME-Name": "PMPCIECam00", "640-DA Device": "640 Shutter", "405 AO-Block voltage": "false", "ElapsedTime-ms": 1396153, "PRIME-Offset": "100", "445 AO-IOChannel": "Dev1/ao1", "640 AO-MaxVolts": "5.0000", "PositionIndex": 34, "PRIME-PP 1 ENABLED": "Yes", "COM1-BaudRate": "9600", "640-State": "0", "COM3-Verbose": "1", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "LudlController-TransmissionDelay": "0", "PRIME-FullWellCapacity": "62000", "PRIME-SMARTStreamingEnabled": "No", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "640 Shutter-Description": "NI DAC", "528-Description": "DA device that is used as a shutter", "COM4-Description": "Serial port driver (boost:asio)", "405 Shutter-Description": "NI DAC", "TE2000-Name": "TE2000", "528 Shutter-Description": "NI DAC", "COM9-Description": "Serial port driver (boost:asio)", "LudlController-Name": "LudlController", "445 Shutter-TriggerSequenceLength": "1000", "PRIME-SerialNumber": "A18B203008", "445 AO-Volts": "0.0000", "PRIME-X-dimension": "1200", "PRIME-ShutterCloseDelay": "0", "XLIGHT Disk slider-State": "2", "Position": "Default", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 AO-IOChannel": "Dev1/ao5", "520 AO-IOChannel": "Dev1/ao4", "528 AO-TriggerSequenceLength": "1000", "405 Shutter-MaxVolts": "5.0000", "470-DA Device": "470 Shutter", "PRIME-FTCapable": "No", "Core-ChannelGroup": "Channels", "555 AO-SupportsTriggering": "0", "PRIME-Y-dimension": "1200", "Frame": 0, "555 Shutter-Sequenceable": "1", "PRIME-Timing-ExposureTimeNs": "499998000.0000", "Core-ImageProcessor": "", "528-State": "0", "520 AO-Block voltage": "false", "XLIGHT Excitation Wheel-Label": "CFP/YFP", "640 Shutter-Demo": "No", "COM3-StopBits": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "520 Shutter-IOChannel": "Dev1/ao11", "Width": 1200, "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-SupportsTriggering": "0", "640 AO-TriggerSampleRate": "100000", "520-Description": "DA device that is used as a shutter", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "405 Shutter-Sequenceable": "1", "405 Shutter-SupportsTriggering": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-TriggerSampleRate": "100000", "640 Shutter-MaxVolts": "5.0000", "555 AO-MaxVolts": "5.0000", "520 AO-TriggerSequenceLength": "1000", "COM3-BaudRate": "9600", "COM9-BaudRate": "9600", "Vincent-D3-Channel #": "Ch. #1", "PRIME-PMode": "Normal", "OpticalPath-Label": "Left(100)", "BitDepth": 16, "555 AO-TriggerSequenceLength": "1000", "445 Shutter-Description": "NI DAC", "405 AO-Sequenceable": "1", "PRIME-AcquisitionMethod": "Callbacks", "555 AO-Name": "AnalogIO", "PRIME-ShutterMode": "Pre-Exposure", "COM3-Handshaking": "Off", "445 AO-SupportsTriggering": "0", "ROI": "0-0-1200-1200", "COM9-Verbose": "1", "PRIME-Color": "OFF", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "PRIME-ShutterOpenDelay": "0", "COM1-Parity": "None", "Slice": 9, "SlicePosition": 40, "COM9-DelayBetweenCharsMs": "0.0000", "555-Name": "DA Shutter", "PIEZO-LudlSingleAxisName": "B", "XLIGHT Emission Wheel-Label": "565/24(G)", "640 Shutter-Name": "AnalogIO", "640 AO-Sequenceable": "1", "520 Shutter-Demo": "No", "528 Shutter-Demo": "No", "470-Name": "DA Shutter", "520-DA Device": "520 Shutter", "XYStage-Description": "Ludl XY stage driver adapter", "445 Shutter-MinVolts": "0.0000", "528 AO-TriggerSampleRate": "100000", "405 AO-MinVolts": "0.0000", "405 Shutter-TriggerSequenceLength": "1000", "Height": 1200, "555 AO-Block voltage": "false", "640 Shutter-Volts": "0.0000", "PixelType": "GRAY16", "445 Shutter-Block voltage": "false", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "520 Shutter-TriggerSequenceLength": "1000", "COM9-Name": "COM9", "Core-SLM": "", "555 AO-Volts": "0.0000", "555 Shutter-Block voltage": "false", "XYStage-Speed": "1800.0000", "520 AO-MaxVolts": "5.0000", "528 Shutter-SupportsTriggering": "0", "COM1-Description": "Serial port driver (boost:asio)", "PRIME-MetadataEnabled": "No", "520 Shutter-MinVolts": "0.0000", "470 Shutter-Sequenceable": "1", "PRIME-PreampDelay": "0", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-MinVolts": "0.0000", "ChannelIndex": 0, "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Core-AutoShutter": "1", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "Exposure-ms": 320, "PRIME-PP 2 ENABLED": "Yes", "640 Shutter-TriggerSampleRate": "100000", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "470 Shutter-IOChannel": "Dev1/ao10", "405 AO-Demo": "No", "PixelSizeUm": 1.1, "470 AO-Demo": "No", "470 Shutter-MaxVolts": "5.0000", "Vincent-D3-Name": "Vincent-D3", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FileName": "img_000000000_Il-G_009.tif", "LudlController-Reset": "Operate", "PRIME-Exposure": "320.00", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "445-Name": "DA Shutter", "555 Shutter-Name": "AnalogIO", "520 Shutter-TriggerSampleRate": "100000", "445 Shutter-IOChannel": "Dev1/ao9", "PRIME-TransposeCorrection": "0", "XYStage-StepSize": "0.1000", "445-Description": "DA device that is used as a shutter", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 AO-IOChannel": "Dev1/ao7", "520 Shutter-Name": "AnalogIO", "COM3-DataBits": "8", "640 AO-Volts": "0.0000", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "555 Shutter-IOChannel": "Dev1/ao13", "WaitInterval": null, "520 Shutter-Sequenceable": "1", "YPositionUm": 545.6, "445-State": "0", "445-DA Device": "445 Shutter", "PRIME-CircularBufferAutoSize": "ON", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "555 AO-TriggerSampleRate": "100000", "445 Shutter-Volts": "0.0000", "XLIGHT Hub-Port": "COM3", "Core-AutoFocus": "", "COM4-Handshaking": "Off", "528 Shutter-MinVolts": "0.0000", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "445 Shutter-Name": "AnalogIO", "528 Shutter-Sequenceable": "1", "XYStage-TransposeMirrorX": "0", "XYStage-TransposeMirrorY": "0", "PRIME-ReadoutRate": "100MHz 16bit", "XYStage-Name": "Stage", "640 AO-SupportsTriggering": "0", "640-Name": "DA Shutter", "640 Shutter-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "640 AO-Block voltage": "false", "445 Shutter-Sequenceable": "1", "470 AO-MaxVolts": "5.0000", "COM4-BaudRate": "9600", "470-Description": "DA device that is used as a shutter", "520 Shutter-Description": "NI DAC", "PRIME-CCDTemperature": "-14.8500", "Time": "2019-12-19 09:59:26 -0500", "640 AO-TriggerSequenceLength": "1000", "470 AO-Name": "AnalogIO", "XLIGHT Disk slider-Label": "Disk Pos 40um", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "Binning": "1x1", "TE2000-ModelType": "1", "COM3-AnswerTimeout": "500.0000", "555 Shutter-SupportsTriggering": "0", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "TE2000-Port": "COM4", "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Description": "NI DAC", "COM4-AnswerTimeout": "500.0000", "COM1-DelayBetweenCharsMs": "0.0000", "FilterBlock-Label": "Confocal", "445 AO-Name": "AnalogIO", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-Timing-ReadoutTimeNs": "24672000.0000", "528 AO-Demo": "No", "520 AO-Sequenceable": "1", "555-Description": "DA device that is used as a shutter", "405-DA Device": "405 Shutter", "405 AO-TriggerSequenceLength": "1000", "640 AO-MinVolts": "0.0000", "640 Shutter-Block voltage": "false", "FrameIndex": 0, "PRIME-Port": "CMOS", "555 AO-MinVolts": "0.0000", "COM3-Description": "Serial port driver (boost:asio)", "528 AO-Description": "NI DAC", "555 AO-IOChannel": "Dev1/ao6", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "555 AO-TriggerInputLine": "/Dev1/PFI0", "PRIME-ActualInterval-ms": "390.5200", "LudlController-Port": "COM9", "445 Shutter-Demo": "No", "PRIME-Trigger-Expose Out-Mux": "1", "SliceIndex": 9, "PRIME-CCDTemperatureSetPoint": "-15.0000", "405 Shutter-MinVolts": "0.0000", "PRIME-ExposeOutMode": "First Row", "AxisPositions": null, "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Volts": "0.0000", "528-Name": "DA Shutter", "555-State": "0", "XLIGHT Dichroic-Label": "CFP/YFP", "445 AO-Sequenceable": "1", "405-Name": "DA Shutter", "405 AO-IOChannel": "Dev1/ao0", "Core-Camera": "PRIME", "Focus-Name": "Focus", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "405 Shutter-IOChannel": "Dev1/ao8", "640 AO-Demo": "No", "640 Shutter-SupportsTriggering": "0", "445 AO-Demo": "No", "555-DA Device": "555 Shutter", "470 Shutter-Description": "NI DAC", "528 AO-Volts": "0.0000", "COM1-DataBits": "8", "Vincent-D3-Address": "x", "520 AO-SupportsTriggering": "0", "405-Description": "DA device that is used as a shutter", "640 Shutter-MinVolts": "0.0000", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "405 AO-Name": "AnalogIO", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "640 AO-TriggerInputLine": "/Dev1/PFI0", "405 AO-TriggerInputLine": "/Dev1/PFI0", "640-Description": "DA device that is used as a shutter", "445 Shutter-TriggerSampleRate": "100000", "470 Shutter-TriggerSequenceLength": "1000", "COM1-StopBits": "1", "520 Shutter-SupportsTriggering": "0", "405-State": "0", "Core-Initialize": "1", "XLIGHT Emission Wheel-State": "3", "520 AO-Description": "NI DAC", "528 AO-TriggerInputLine": "/Dev1/PFI0", "445 Shutter-MaxVolts": "5.0000", "COM9-Handshaking": "Off", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "XLIGHT Spin motor-Label": "On (spinning)", "PRIME-PP 5 ENABLED": "No", "UUID": "62b16247-12ea-4037-826f-073c3a12b0b1", "PRIME-TriggerMode": "Internal Trigger", "COM1-Handshaking": "Off", "PRIME-Timing-PostTriggerDelayNs": "49344000.0000", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "Focus-Position": "17741.5000", "528 Shutter-Block voltage": "false", "Channel": "Il-G", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "555 Shutter-Demo": "No", "405 Shutter-Demo": "No", "528 AO-Name": "AnalogIO", "520-State": "1", "Core-Galvo": "", "405 Shutter-TriggerSampleRate": "100000", "Focus-FocusStepSize_(nm)": "100", "PRIME-PixelType": "16bit", "COM4-DataBits": "8", "470 AO-IOChannel": "Dev1/ao2", "520 AO-MinVolts": "0.0000", "COM9-Parity": "None", "PRIME-CircularBufferEnabled": "ON", "PRIME-PP 6 Reset": "No", "640 AO-Description": "NI DAC", "Source": "PRIME", "445 AO-MinVolts": "0.0000", "PIEZO-Name": "Stage", "445 AO-Description": "NI DAC", "PRIME-CircularBufferFrameCount": "46", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "520 AO-Volts": "4.5000", "528 Shutter-TriggerSampleRate": "100000", "XPositionUm": -1324.9, "OpticalPath-State": "4", "PRIME-PP 3 THRESHOLD": "80", "PRIME-PVCAM Version": "3.7.1", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Gain": "1-HDR", "640 Shutter-TriggerSequenceLength": "1000", "528 AO-SupportsTriggering": "0", "445 AO-TriggerInputLine": "/Dev1/PFI0", "OpticalPath-Name": "OpticalPath", "PRIME-PP 4 ENABLED": "Yes", "PRIME-PVCAM Adapter Version": "1.3.29", "COM3-Name": "COM3", "405 AO-MaxVolts": "5.0000", "520 Shutter-Block voltage": "false", "PRIME-Timing-ClearingTimeNs": "49344000.0000", "Core-Focus": "PIEZO", "528 AO-Block voltage": "false", "TE2000-Description": "Nikon TE2000 hub", "528 AO-MinVolts": "0.0000", "COM4-Parity": "None", "Summary": { "Slices": 11, "ChContrastMin": [ 0, 0, 0, 0, 0, 0 ], "PixelAspect": 1, "SlicesFirst": true, "Interval_ms": 0, "Time": "2019-12-19 09:59:21 -0500", "Prefix": "seq_13", "InitialPositionList": [ { "Label": "Pos0", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.21], "Focus": [17741.5], "XYStage": [ 44542.200000000004, 31636.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos1", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.22], "Focus": [17741.4], "XYStage": [ 43801.100000000006, 31637.9 ] }, "GridColumnIndex": 0 }, { "Label": "Pos2", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.5], "XYStage": [ 38561.3, 32040.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos3", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.185], "Focus": [17741.3], "XYStage": [ 37698.4, 32041.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos4", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.16], "Focus": [17741.5], "XYStage": [ 32375.4, 31758.9 ] }, "GridColumnIndex": 0 }, { "Label": "Pos5", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.17000000000002], "Focus": [17741.3], "XYStage": [ 31770.7, 31758.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos6", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.4], "XYStage": [ 26536.800000000003, 31068.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos7", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.205], "Focus": [17741.4], "XYStage": [ 25420.2, 31068.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos8", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.85], "Focus": [17741.3], "XYStage": [ 21059, 31508 ] }, "GridColumnIndex": 0 }, { "Label": "Pos9", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.82], "Focus": [17741.3], "XYStage": [ 19731.9, 31506.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos10", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.79], "Focus": [17741.4], "XYStage": [ 14528.5, 32007.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos11", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.83], "Focus": [17741.2], "XYStage": [ 13830.300000000001, 32009.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos12", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.79], "Focus": [17741.3], "XYStage": [ 8775.300000000001, 32017.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos13", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.815], "Focus": [17741.3], "XYStage": [ 8109.200000000001, 32008.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos14", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.805], "Focus": [17741.3], "XYStage": [ 3483.3, 31908.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos15", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.82], "Focus": [17741.2], "XYStage": [ 2686.8, 31903.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos16", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [211.48000000000002], "Focus": [17741.1], "XYStage": [ -1978.9, 31973.600000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos17", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [211.51500000000001], "Focus": [17741.1], "XYStage": [ -2957.8, 31982.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos18", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.195], "Focus": [17741.5], "XYStage": [ 47747.4, -576.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos19", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.23000000000002], "Focus": [17741.6], "XYStage": [ 47053.9, -576.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos20", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.205], "Focus": [17741.4], "XYStage": [ 41871.200000000004, -479.8 ] }, "GridColumnIndex": 0 }, { "Label": "Pos21", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.4], "XYStage": [ 40882.600000000006, -392 ] }, "GridColumnIndex": 0 }, { "Label": "Pos22", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.24], "Focus": [17741.4], "XYStage": [ 35905.6, -135.3 ] }, "GridColumnIndex": 0 }, { "Label": "Pos23", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.195], "Focus": [17741.5], "XYStage": [ 34990.9, -132.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos24", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.46], "Focus": [17741.4], "XYStage": [ 29678.100000000002, -958.1 ] }, "GridColumnIndex": 0 }, { "Label": "Pos25", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.44], "Focus": [17741.5], "XYStage": [ 28983.600000000002, -939.4000000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos26", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.475], "Focus": [17741.5], "XYStage": [ 23019.7, -122 ] }, "GridColumnIndex": 0 }, { "Label": "Pos27", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.44], "Focus": [17741.7], "XYStage": [ 22428, -116.80000000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos28", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.46], "Focus": [17741.7], "XYStage": [ 16364.900000000001, -181.10000000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos29", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.48000000000002], "Focus": [17741.5], "XYStage": [ 15712.1, -180 ] }, "GridColumnIndex": 0 }, { "Label": "Pos30", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.45000000000002], "Focus": [17741.7], "XYStage": [ 10346.1, 77.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos31", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.49], "Focus": [17741.4], "XYStage": [ 9755.5, 85.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos32", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.475], "Focus": [17741.5], "XYStage": [ 4775.5, 293.90000000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos33", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.445], "Focus": [17741.4], "XYStage": [ 3954.9, 293.90000000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos34", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.455], "Focus": [17741.4], "XYStage": [ -1324.9, 545.6 ] }, "GridColumnIndex": 0 }, { "Label": "Pos35", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.445], "Focus": [17741.3], "XYStage": [ -2097, 576.6 ] }, "GridColumnIndex": 0 } ], "z-step_um": 10, "GridRow": 0, "BitDepth": 16, "Depth": 2, "Channels": 6, "Source": "Micro-Manager", "ChColors": [ -16711681, -256, -65281, -1, -1, -16776961 ], "MetadataVersion": 10, "KeepShutterOpenChannels": true, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536, 65536 ], "CustomIntervals_ms": [], "Height": 1200, "MicroManagerVersion": "1.4.23 20180219", "GridColumn": 0, "UUID": "18808e7c-f2e6-47b3-90d8-1d779ce87dbd", "PixelType": "GRAY16", "Width": 1200, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\Yan\\20191218-YC113-S7S8-miseq", "Frames": 1, "Comment": "", "UserName": "Nikon", "PixelSize_um": 1.1, "ROI": [ 0, 0, 1200, 1200 ], "Date": "2019-12-19", "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC", "P-DAPI-high" ], "KeepShutterOpenSlices": true, "IJType": 1, "ComputerName": "ZadorConfocal2", "Positions": 36, "PositionIndex": 34, "CameraTimeout": 20000, "TimeFirst": false }, "640 AO-Name": "AnalogIO", "Vincent-D3-Command": "Close" }, "FrameKey-0-0-10": { "COM4-StopBits": "1", "528 AO-MaxVolts": "5.0000", "XLIGHT Excitation Wheel-State": "1", "470 Shutter-Name": "AnalogIO", "Core-Shutter": "520", "555 AO-Demo": "No", "XLIGHT Dichroic-State": "2", "XYStage-StepSize-Y": "0.1000", "ZPositionUm": 268.45500000000004, "XYStage-StepSize-X": "0.1000", "PRIME-FirmwareVersion": "20.28", "PRIME-ClearCycles": "2", "520-Name": "DA Shutter", "405 Shutter-Name": "AnalogIO", "PIEZO-StepSize": "0.0050", "PRIME-ClearMode": "Pre-Exposure", "470 AO-MinVolts": "0.0000", "405 AO-SupportsTriggering": "1", "CameraChannelIndex": 0, "XYStage-StartSpeed": "500.0000", "COM9-StopBits": "2", "405 AO-Description": "NI DAC", "COM4-DelayBetweenCharsMs": "0.0000", "445 AO-Block voltage": "false", "520 AO-TriggerInputLine": "/Dev1/PFI0", "555 AO-Description": "NI DAC", "520 AO-TriggerSampleRate": "100000", "COM4-Verbose": "1", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM4-Name": "COM4", "405 Shutter-Volts": "0.0000", "Vincent-D3-Port": "COM1", "PRIME-PP 4 MIN ADU AFFECTED": "200", "470 Shutter-MinVolts": "0.0000", "PRIME-PP 3 ENABLED": "Yes", "470 AO-Description": "NI DAC", "PRIME-Description": "PVCAM API device adapter", "520 Shutter-MaxVolts": "5.0000", "520 Shutter-Volts": "5.0000", "528 AO-Sequenceable": "1", "PRIME-Binning": "1x1", "555 Shutter-MaxVolts": "5.0000", "528 Shutter-TriggerSequenceLength": "1000", "405 Shutter-Block voltage": "false", "XLIGHT Spin motor-State": "1", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "Vincent-D3-Time to open (ms)": "35.0000", "Vincent-D3-Time to close (ms)": "35.0000", "445 AO-TriggerSampleRate": "100000", "640 Shutter-IOChannel": "Dev1/ao14", "555 AO-Sequenceable": "1", "520 AO-Name": "AnalogIO", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "470 AO-Block voltage": "false", "COM1-Verbose": "1", "COM9-AnswerTimeout": "10000.0000", "528 Shutter-Name": "AnalogIO", "PRIME-Trigger Timeout (secs)": "10", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "NextFrame": 0, "470 AO-TriggerSequenceLength": "1000", "PRIME-TransposeXY": "0", "445 AO-TriggerSequenceLength": "1000", "COM1-Name": "COM1", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "COM9-DataBits": "8", "COM1-AnswerTimeout": "500.0000", "Camera": "", "TE2000-Version": "V2.50-V2.50-V2.10", "528 Shutter-Volts": "0.0000", "XLIGHT Hub-Name": "XLIGHT Hub", "PIEZO-Description": "Ludl stage driver adapter", "PositionName": "Pos34", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSampleRate": "100000", "PRIME-FanSpeedSetpoint": "High", "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "405 AO-TriggerSampleRate": "100000", "470 Shutter-Block voltage": "false", "470-State": "0", "COM3-Parity": "None", "405 AO-Volts": "0.0000", "PRIME-TransposeMirrorY": "0", "PRIME-ChipName": "GS144BSI", "470 AO-Volts": "0.0000", "PRIME-TransposeMirrorX": "0", "445 Shutter-SupportsTriggering": "0", "470 Shutter-SupportsTriggering": "0", "XLIGHT Hub-Description": "XLIGHT hub", "PRIME-PreampOffLimit": "10000", "470 AO-Sequenceable": "1", "528 Shutter-MaxVolts": "5.0000", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "Core-XYStage": "XYStage", "470 AO-TriggerSampleRate": "100000", "PRIME-Timing-PreTriggerDelayNs": "24672000.0000", "528-DA Device": "528 Shutter", "Focus-Description": "Nikon TE2000 focus adapter", "FilterBlock-State": "5", "520 AO-Demo": "No", "COM3-DelayBetweenCharsMs": "0.0000", "470 Shutter-Volts": "0.0000", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "PRIME-PP 2 THRESHOLD": "125", "PRIME-PP 4 THRESHOLD": "75", "XYStage-Acceleration": "4.0000", "470 Shutter-Demo": "No", "PRIME-Name": "PMPCIECam00", "640-DA Device": "640 Shutter", "405 AO-Block voltage": "false", "ElapsedTime-ms": 1396765, "PRIME-Offset": "100", "445 AO-IOChannel": "Dev1/ao1", "640 AO-MaxVolts": "5.0000", "PositionIndex": 34, "PRIME-PP 1 ENABLED": "Yes", "COM1-BaudRate": "9600", "640-State": "0", "COM3-Verbose": "1", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "LudlController-TransmissionDelay": "0", "PRIME-FullWellCapacity": "62000", "PRIME-SMARTStreamingEnabled": "No", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "640 Shutter-Description": "NI DAC", "528-Description": "DA device that is used as a shutter", "COM4-Description": "Serial port driver (boost:asio)", "405 Shutter-Description": "NI DAC", "TE2000-Name": "TE2000", "528 Shutter-Description": "NI DAC", "COM9-Description": "Serial port driver (boost:asio)", "LudlController-Name": "LudlController", "445 Shutter-TriggerSequenceLength": "1000", "PRIME-SerialNumber": "A18B203008", "445 AO-Volts": "0.0000", "PRIME-X-dimension": "1200", "PRIME-ShutterCloseDelay": "0", "XLIGHT Disk slider-State": "2", "Position": "Default", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 AO-IOChannel": "Dev1/ao5", "520 AO-IOChannel": "Dev1/ao4", "528 AO-TriggerSequenceLength": "1000", "405 Shutter-MaxVolts": "5.0000", "470-DA Device": "470 Shutter", "PRIME-FTCapable": "No", "Core-ChannelGroup": "Channels", "555 AO-SupportsTriggering": "0", "PRIME-Y-dimension": "1200", "Frame": 0, "555 Shutter-Sequenceable": "1", "PRIME-Timing-ExposureTimeNs": "499998000.0000", "Core-ImageProcessor": "", "528-State": "0", "520 AO-Block voltage": "false", "XLIGHT Excitation Wheel-Label": "CFP/YFP", "640 Shutter-Demo": "No", "COM3-StopBits": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "520 Shutter-IOChannel": "Dev1/ao11", "Width": 1200, "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-SupportsTriggering": "0", "640 AO-TriggerSampleRate": "100000", "520-Description": "DA device that is used as a shutter", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "405 Shutter-Sequenceable": "1", "405 Shutter-SupportsTriggering": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-TriggerSampleRate": "100000", "640 Shutter-MaxVolts": "5.0000", "555 AO-MaxVolts": "5.0000", "520 AO-TriggerSequenceLength": "1000", "COM3-BaudRate": "9600", "COM9-BaudRate": "9600", "Vincent-D3-Channel #": "Ch. #1", "PRIME-PMode": "Normal", "OpticalPath-Label": "Left(100)", "BitDepth": 16, "555 AO-TriggerSequenceLength": "1000", "445 Shutter-Description": "NI DAC", "405 AO-Sequenceable": "1", "PRIME-AcquisitionMethod": "Callbacks", "555 AO-Name": "AnalogIO", "PRIME-ShutterMode": "Pre-Exposure", "COM3-Handshaking": "Off", "445 AO-SupportsTriggering": "0", "ROI": "0-0-1200-1200", "COM9-Verbose": "1", "PRIME-Color": "OFF", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "PRIME-ShutterOpenDelay": "0", "COM1-Parity": "None", "Slice": 10, "SlicePosition": 50, "COM9-DelayBetweenCharsMs": "0.0000", "555-Name": "DA Shutter", "PIEZO-LudlSingleAxisName": "B", "XLIGHT Emission Wheel-Label": "565/24(G)", "640 Shutter-Name": "AnalogIO", "640 AO-Sequenceable": "1", "520 Shutter-Demo": "No", "528 Shutter-Demo": "No", "470-Name": "DA Shutter", "520-DA Device": "520 Shutter", "XYStage-Description": "Ludl XY stage driver adapter", "445 Shutter-MinVolts": "0.0000", "528 AO-TriggerSampleRate": "100000", "405 AO-MinVolts": "0.0000", "405 Shutter-TriggerSequenceLength": "1000", "Height": 1200, "555 AO-Block voltage": "false", "640 Shutter-Volts": "0.0000", "PixelType": "GRAY16", "445 Shutter-Block voltage": "false", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "520 Shutter-TriggerSequenceLength": "1000", "COM9-Name": "COM9", "Core-SLM": "", "555 AO-Volts": "0.0000", "555 Shutter-Block voltage": "false", "XYStage-Speed": "1800.0000", "520 AO-MaxVolts": "5.0000", "528 Shutter-SupportsTriggering": "0", "COM1-Description": "Serial port driver (boost:asio)", "PRIME-MetadataEnabled": "No", "520 Shutter-MinVolts": "0.0000", "470 Shutter-Sequenceable": "1", "PRIME-PreampDelay": "0", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-MinVolts": "0.0000", "ChannelIndex": 0, "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Core-AutoShutter": "1", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "Exposure-ms": 320, "PRIME-PP 2 ENABLED": "Yes", "640 Shutter-TriggerSampleRate": "100000", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "470 Shutter-IOChannel": "Dev1/ao10", "405 AO-Demo": "No", "PixelSizeUm": 1.1, "470 AO-Demo": "No", "470 Shutter-MaxVolts": "5.0000", "Vincent-D3-Name": "Vincent-D3", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FileName": "img_000000000_Il-G_010.tif", "LudlController-Reset": "Operate", "PRIME-Exposure": "320.00", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "445-Name": "DA Shutter", "555 Shutter-Name": "AnalogIO", "520 Shutter-TriggerSampleRate": "100000", "445 Shutter-IOChannel": "Dev1/ao9", "PRIME-TransposeCorrection": "0", "XYStage-StepSize": "0.1000", "445-Description": "DA device that is used as a shutter", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 AO-IOChannel": "Dev1/ao7", "520 Shutter-Name": "AnalogIO", "COM3-DataBits": "8", "640 AO-Volts": "0.0000", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "555 Shutter-IOChannel": "Dev1/ao13", "WaitInterval": null, "520 Shutter-Sequenceable": "1", "YPositionUm": 545.6, "445-State": "0", "445-DA Device": "445 Shutter", "PRIME-CircularBufferAutoSize": "ON", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "555 AO-TriggerSampleRate": "100000", "445 Shutter-Volts": "0.0000", "XLIGHT Hub-Port": "COM3", "Core-AutoFocus": "", "COM4-Handshaking": "Off", "528 Shutter-MinVolts": "0.0000", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "445 Shutter-Name": "AnalogIO", "528 Shutter-Sequenceable": "1", "XYStage-TransposeMirrorX": "0", "XYStage-TransposeMirrorY": "0", "PRIME-ReadoutRate": "100MHz 16bit", "XYStage-Name": "Stage", "640 AO-SupportsTriggering": "0", "640-Name": "DA Shutter", "640 Shutter-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "640 AO-Block voltage": "false", "445 Shutter-Sequenceable": "1", "470 AO-MaxVolts": "5.0000", "COM4-BaudRate": "9600", "470-Description": "DA device that is used as a shutter", "520 Shutter-Description": "NI DAC", "PRIME-CCDTemperature": "-14.8500", "Time": "2019-12-19 09:59:27 -0500", "640 AO-TriggerSequenceLength": "1000", "470 AO-Name": "AnalogIO", "XLIGHT Disk slider-Label": "Disk Pos 40um", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "Binning": "1x1", "TE2000-ModelType": "1", "COM3-AnswerTimeout": "500.0000", "555 Shutter-SupportsTriggering": "0", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "TE2000-Port": "COM4", "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Description": "NI DAC", "COM4-AnswerTimeout": "500.0000", "COM1-DelayBetweenCharsMs": "0.0000", "FilterBlock-Label": "Confocal", "445 AO-Name": "AnalogIO", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-Timing-ReadoutTimeNs": "24672000.0000", "528 AO-Demo": "No", "520 AO-Sequenceable": "1", "555-Description": "DA device that is used as a shutter", "405-DA Device": "405 Shutter", "405 AO-TriggerSequenceLength": "1000", "640 AO-MinVolts": "0.0000", "640 Shutter-Block voltage": "false", "FrameIndex": 0, "PRIME-Port": "CMOS", "555 AO-MinVolts": "0.0000", "COM3-Description": "Serial port driver (boost:asio)", "528 AO-Description": "NI DAC", "555 AO-IOChannel": "Dev1/ao6", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "555 AO-TriggerInputLine": "/Dev1/PFI0", "PRIME-ActualInterval-ms": "390.5200", "LudlController-Port": "COM9", "445 Shutter-Demo": "No", "PRIME-Trigger-Expose Out-Mux": "1", "SliceIndex": 10, "PRIME-CCDTemperatureSetPoint": "-15.0000", "405 Shutter-MinVolts": "0.0000", "PRIME-ExposeOutMode": "First Row", "AxisPositions": null, "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Volts": "0.0000", "528-Name": "DA Shutter", "555-State": "0", "XLIGHT Dichroic-Label": "CFP/YFP", "445 AO-Sequenceable": "1", "405-Name": "DA Shutter", "405 AO-IOChannel": "Dev1/ao0", "Core-Camera": "PRIME", "Focus-Name": "Focus", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "405 Shutter-IOChannel": "Dev1/ao8", "640 AO-Demo": "No", "640 Shutter-SupportsTriggering": "0", "445 AO-Demo": "No", "555-DA Device": "555 Shutter", "470 Shutter-Description": "NI DAC", "528 AO-Volts": "0.0000", "COM1-DataBits": "8", "Vincent-D3-Address": "x", "520 AO-SupportsTriggering": "0", "405-Description": "DA device that is used as a shutter", "640 Shutter-MinVolts": "0.0000", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "405 AO-Name": "AnalogIO", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "640 AO-TriggerInputLine": "/Dev1/PFI0", "405 AO-TriggerInputLine": "/Dev1/PFI0", "640-Description": "DA device that is used as a shutter", "445 Shutter-TriggerSampleRate": "100000", "470 Shutter-TriggerSequenceLength": "1000", "COM1-StopBits": "1", "520 Shutter-SupportsTriggering": "0", "405-State": "0", "Core-Initialize": "1", "XLIGHT Emission Wheel-State": "3", "520 AO-Description": "NI DAC", "528 AO-TriggerInputLine": "/Dev1/PFI0", "445 Shutter-MaxVolts": "5.0000", "COM9-Handshaking": "Off", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "XLIGHT Spin motor-Label": "On (spinning)", "PRIME-PP 5 ENABLED": "No", "UUID": "d2bc2a10-fd60-4da2-9bde-823af1913d04", "PRIME-TriggerMode": "Internal Trigger", "COM1-Handshaking": "Off", "PRIME-Timing-PostTriggerDelayNs": "49344000.0000", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "Focus-Position": "17741.5000", "528 Shutter-Block voltage": "false", "Channel": "Il-G", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "555 Shutter-Demo": "No", "405 Shutter-Demo": "No", "528 AO-Name": "AnalogIO", "520-State": "0", "Core-Galvo": "", "405 Shutter-TriggerSampleRate": "100000", "Focus-FocusStepSize_(nm)": "100", "PRIME-PixelType": "16bit", "COM4-DataBits": "8", "470 AO-IOChannel": "Dev1/ao2", "520 AO-MinVolts": "0.0000", "COM9-Parity": "None", "PRIME-CircularBufferEnabled": "ON", "PRIME-PP 6 Reset": "No", "640 AO-Description": "NI DAC", "Source": "PRIME", "445 AO-MinVolts": "0.0000", "PIEZO-Name": "Stage", "445 AO-Description": "NI DAC", "PRIME-CircularBufferFrameCount": "46", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "520 AO-Volts": "4.5000", "528 Shutter-TriggerSampleRate": "100000", "XPositionUm": -1324.9, "OpticalPath-State": "4", "PRIME-PP 3 THRESHOLD": "80", "PRIME-PVCAM Version": "3.7.1", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Gain": "1-HDR", "640 Shutter-TriggerSequenceLength": "1000", "528 AO-SupportsTriggering": "0", "445 AO-TriggerInputLine": "/Dev1/PFI0", "OpticalPath-Name": "OpticalPath", "PRIME-PP 4 ENABLED": "Yes", "PRIME-PVCAM Adapter Version": "1.3.29", "COM3-Name": "COM3", "405 AO-MaxVolts": "5.0000", "520 Shutter-Block voltage": "false", "PRIME-Timing-ClearingTimeNs": "49344000.0000", "Core-Focus": "PIEZO", "528 AO-Block voltage": "false", "TE2000-Description": "Nikon TE2000 hub", "528 AO-MinVolts": "0.0000", "COM4-Parity": "None", "Summary": { "Slices": 11, "ChContrastMin": [ 0, 0, 0, 0, 0, 0 ], "PixelAspect": 1, "SlicesFirst": true, "Interval_ms": 0, "Time": "2019-12-19 09:59:21 -0500", "Prefix": "seq_13", "InitialPositionList": [ { "Label": "Pos0", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.21], "Focus": [17741.5], "XYStage": [ 44542.200000000004, 31636.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos1", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.22], "Focus": [17741.4], "XYStage": [ 43801.100000000006, 31637.9 ] }, "GridColumnIndex": 0 }, { "Label": "Pos2", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.5], "XYStage": [ 38561.3, 32040.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos3", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.185], "Focus": [17741.3], "XYStage": [ 37698.4, 32041.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos4", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.16], "Focus": [17741.5], "XYStage": [ 32375.4, 31758.9 ] }, "GridColumnIndex": 0 }, { "Label": "Pos5", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.17000000000002], "Focus": [17741.3], "XYStage": [ 31770.7, 31758.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos6", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.4], "XYStage": [ 26536.800000000003, 31068.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos7", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.205], "Focus": [17741.4], "XYStage": [ 25420.2, 31068.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos8", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.85], "Focus": [17741.3], "XYStage": [ 21059, 31508 ] }, "GridColumnIndex": 0 }, { "Label": "Pos9", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.82], "Focus": [17741.3], "XYStage": [ 19731.9, 31506.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos10", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.79], "Focus": [17741.4], "XYStage": [ 14528.5, 32007.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos11", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.83], "Focus": [17741.2], "XYStage": [ 13830.300000000001, 32009.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos12", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.79], "Focus": [17741.3], "XYStage": [ 8775.300000000001, 32017.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos13", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.815], "Focus": [17741.3], "XYStage": [ 8109.200000000001, 32008.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos14", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.805], "Focus": [17741.3], "XYStage": [ 3483.3, 31908.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos15", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.82], "Focus": [17741.2], "XYStage": [ 2686.8, 31903.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos16", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [211.48000000000002], "Focus": [17741.1], "XYStage": [ -1978.9, 31973.600000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos17", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [211.51500000000001], "Focus": [17741.1], "XYStage": [ -2957.8, 31982.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos18", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.195], "Focus": [17741.5], "XYStage": [ 47747.4, -576.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos19", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.23000000000002], "Focus": [17741.6], "XYStage": [ 47053.9, -576.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos20", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.205], "Focus": [17741.4], "XYStage": [ 41871.200000000004, -479.8 ] }, "GridColumnIndex": 0 }, { "Label": "Pos21", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.4], "XYStage": [ 40882.600000000006, -392 ] }, "GridColumnIndex": 0 }, { "Label": "Pos22", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.24], "Focus": [17741.4], "XYStage": [ 35905.6, -135.3 ] }, "GridColumnIndex": 0 }, { "Label": "Pos23", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.195], "Focus": [17741.5], "XYStage": [ 34990.9, -132.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos24", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.46], "Focus": [17741.4], "XYStage": [ 29678.100000000002, -958.1 ] }, "GridColumnIndex": 0 }, { "Label": "Pos25", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.44], "Focus": [17741.5], "XYStage": [ 28983.600000000002, -939.4000000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos26", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.475], "Focus": [17741.5], "XYStage": [ 23019.7, -122 ] }, "GridColumnIndex": 0 }, { "Label": "Pos27", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.44], "Focus": [17741.7], "XYStage": [ 22428, -116.80000000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos28", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.46], "Focus": [17741.7], "XYStage": [ 16364.900000000001, -181.10000000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos29", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.48000000000002], "Focus": [17741.5], "XYStage": [ 15712.1, -180 ] }, "GridColumnIndex": 0 }, { "Label": "Pos30", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.45000000000002], "Focus": [17741.7], "XYStage": [ 10346.1, 77.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos31", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.49], "Focus": [17741.4], "XYStage": [ 9755.5, 85.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos32", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.475], "Focus": [17741.5], "XYStage": [ 4775.5, 293.90000000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos33", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.445], "Focus": [17741.4], "XYStage": [ 3954.9, 293.90000000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos34", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.455], "Focus": [17741.4], "XYStage": [ -1324.9, 545.6 ] }, "GridColumnIndex": 0 }, { "Label": "Pos35", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.445], "Focus": [17741.3], "XYStage": [ -2097, 576.6 ] }, "GridColumnIndex": 0 } ], "z-step_um": 10, "GridRow": 0, "BitDepth": 16, "Depth": 2, "Channels": 6, "Source": "Micro-Manager", "ChColors": [ -16711681, -256, -65281, -1, -1, -16776961 ], "MetadataVersion": 10, "KeepShutterOpenChannels": true, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536, 65536 ], "CustomIntervals_ms": [], "Height": 1200, "MicroManagerVersion": "1.4.23 20180219", "GridColumn": 0, "UUID": "18808e7c-f2e6-47b3-90d8-1d779ce87dbd", "PixelType": "GRAY16", "Width": 1200, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\Yan\\20191218-YC113-S7S8-miseq", "Frames": 1, "Comment": "", "UserName": "Nikon", "PixelSize_um": 1.1, "ROI": [ 0, 0, 1200, 1200 ], "Date": "2019-12-19", "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC", "P-DAPI-high" ], "KeepShutterOpenSlices": true, "IJType": 1, "ComputerName": "ZadorConfocal2", "Positions": 36, "PositionIndex": 34, "CameraTimeout": 20000, "TimeFirst": false }, "640 AO-Name": "AnalogIO", "Vincent-D3-Command": "Close" }, "FrameKey-0-1-0": { "COM4-StopBits": "1", "528 AO-MaxVolts": "5.0000", "XLIGHT Excitation Wheel-State": "1", "470 Shutter-Name": "AnalogIO", "Core-Shutter": "555", "555 AO-Demo": "No", "XLIGHT Dichroic-State": "2", "XYStage-StepSize-Y": "0.1000", "ZPositionUm": 168.455, "XYStage-StepSize-X": "0.1000", "PRIME-FirmwareVersion": "20.28", "PRIME-ClearCycles": "2", "520-Name": "DA Shutter", "405 Shutter-Name": "AnalogIO", "PIEZO-StepSize": "0.0050", "PRIME-ClearMode": "Pre-Exposure", "470 AO-MinVolts": "0.0000", "405 AO-SupportsTriggering": "1", "CameraChannelIndex": 0, "XYStage-StartSpeed": "500.0000", "COM9-StopBits": "2", "405 AO-Description": "NI DAC", "COM4-DelayBetweenCharsMs": "0.0000", "445 AO-Block voltage": "false", "520 AO-TriggerInputLine": "/Dev1/PFI0", "555 AO-Description": "NI DAC", "520 AO-TriggerSampleRate": "100000", "COM4-Verbose": "1", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM4-Name": "COM4", "405 Shutter-Volts": "0.0000", "Vincent-D3-Port": "COM1", "PRIME-PP 4 MIN ADU AFFECTED": "200", "470 Shutter-MinVolts": "0.0000", "PRIME-PP 3 ENABLED": "Yes", "470 AO-Description": "NI DAC", "PRIME-Description": "PVCAM API device adapter", "520 Shutter-MaxVolts": "5.0000", "520 Shutter-Volts": "0.0000", "528 AO-Sequenceable": "1", "PRIME-Binning": "1x1", "555 Shutter-MaxVolts": "5.0000", "528 Shutter-TriggerSequenceLength": "1000", "405 Shutter-Block voltage": "false", "XLIGHT Spin motor-State": "1", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "Vincent-D3-Time to open (ms)": "35.0000", "Vincent-D3-Time to close (ms)": "35.0000", "445 AO-TriggerSampleRate": "100000", "640 Shutter-IOChannel": "Dev1/ao14", "555 AO-Sequenceable": "1", "520 AO-Name": "AnalogIO", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "470 AO-Block voltage": "false", "COM1-Verbose": "1", "COM9-AnswerTimeout": "10000.0000", "528 Shutter-Name": "AnalogIO", "PRIME-Trigger Timeout (secs)": "10", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "NextFrame": 0, "470 AO-TriggerSequenceLength": "1000", "PRIME-TransposeXY": "0", "445 AO-TriggerSequenceLength": "1000", "COM1-Name": "COM1", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "COM9-DataBits": "8", "COM1-AnswerTimeout": "500.0000", "Camera": "", "TE2000-Version": "V2.50-V2.50-V2.10", "528 Shutter-Volts": "0.0000", "XLIGHT Hub-Name": "XLIGHT Hub", "PIEZO-Description": "Ludl stage driver adapter", "PositionName": "Pos34", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSampleRate": "100000", "PRIME-FanSpeedSetpoint": "High", "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "405 AO-TriggerSampleRate": "100000", "470 Shutter-Block voltage": "false", "470-State": "0", "COM3-Parity": "None", "405 AO-Volts": "0.0000", "PRIME-TransposeMirrorY": "0", "PRIME-ChipName": "GS144BSI", "470 AO-Volts": "0.0000", "PRIME-TransposeMirrorX": "0", "445 Shutter-SupportsTriggering": "0", "470 Shutter-SupportsTriggering": "0", "XLIGHT Hub-Description": "XLIGHT hub", "PRIME-PreampOffLimit": "10000", "470 AO-Sequenceable": "1", "528 Shutter-MaxVolts": "5.0000", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "Core-XYStage": "XYStage", "470 AO-TriggerSampleRate": "100000", "PRIME-Timing-PreTriggerDelayNs": "24672000.0000", "528-DA Device": "528 Shutter", "Focus-Description": "Nikon TE2000 focus adapter", "FilterBlock-State": "5", "520 AO-Demo": "No", "COM3-DelayBetweenCharsMs": "0.0000", "470 Shutter-Volts": "0.0000", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "PRIME-PP 2 THRESHOLD": "125", "PRIME-PP 4 THRESHOLD": "75", "XYStage-Acceleration": "4.0000", "470 Shutter-Demo": "No", "PRIME-Name": "PMPCIECam00", "640-DA Device": "640 Shutter", "405 AO-Block voltage": "false", "ElapsedTime-ms": 1398048, "PRIME-Offset": "100", "445 AO-IOChannel": "Dev1/ao1", "640 AO-MaxVolts": "5.0000", "PositionIndex": 34, "PRIME-PP 1 ENABLED": "Yes", "COM1-BaudRate": "9600", "640-State": "0", "COM3-Verbose": "1", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "LudlController-TransmissionDelay": "0", "PRIME-FullWellCapacity": "62000", "PRIME-SMARTStreamingEnabled": "No", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "640 Shutter-Description": "NI DAC", "528-Description": "DA device that is used as a shutter", "COM4-Description": "Serial port driver (boost:asio)", "405 Shutter-Description": "NI DAC", "TE2000-Name": "TE2000", "528 Shutter-Description": "NI DAC", "COM9-Description": "Serial port driver (boost:asio)", "LudlController-Name": "LudlController", "445 Shutter-TriggerSequenceLength": "1000", "PRIME-SerialNumber": "A18B203008", "445 AO-Volts": "0.0000", "PRIME-X-dimension": "1200", "PRIME-ShutterCloseDelay": "0", "XLIGHT Disk slider-State": "2", "Position": "Default", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 AO-IOChannel": "Dev1/ao5", "520 AO-IOChannel": "Dev1/ao4", "528 AO-TriggerSequenceLength": "1000", "405 Shutter-MaxVolts": "5.0000", "470-DA Device": "470 Shutter", "PRIME-FTCapable": "No", "Core-ChannelGroup": "Channels", "555 AO-SupportsTriggering": "0", "PRIME-Y-dimension": "1200", "Frame": 0, "555 Shutter-Sequenceable": "1", "PRIME-Timing-ExposureTimeNs": "499998000.0000", "Core-ImageProcessor": "", "528-State": "0", "520 AO-Block voltage": "false", "XLIGHT Excitation Wheel-Label": "QUAD", "640 Shutter-Demo": "No", "COM3-StopBits": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "520 Shutter-IOChannel": "Dev1/ao11", "Width": 1200, "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-SupportsTriggering": "0", "640 AO-TriggerSampleRate": "100000", "520-Description": "DA device that is used as a shutter", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "405 Shutter-Sequenceable": "1", "405 Shutter-SupportsTriggering": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-TriggerSampleRate": "100000", "640 Shutter-MaxVolts": "5.0000", "555 AO-MaxVolts": "5.0000", "520 AO-TriggerSequenceLength": "1000", "COM3-BaudRate": "9600", "COM9-BaudRate": "9600", "Vincent-D3-Channel #": "Ch. #1", "PRIME-PMode": "Normal", "OpticalPath-Label": "Left(100)", "BitDepth": 16, "555 AO-TriggerSequenceLength": "1000", "445 Shutter-Description": "NI DAC", "405 AO-Sequenceable": "1", "PRIME-AcquisitionMethod": "Callbacks", "555 AO-Name": "AnalogIO", "PRIME-ShutterMode": "Pre-Exposure", "COM3-Handshaking": "Off", "445 AO-SupportsTriggering": "0", "ROI": "0-0-1200-1200", "COM9-Verbose": "1", "PRIME-Color": "OFF", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "PRIME-ShutterOpenDelay": "0", "COM1-Parity": "None", "Slice": 0, "SlicePosition": -50, "COM9-DelayBetweenCharsMs": "0.0000", "555-Name": "DA Shutter", "PIEZO-LudlSingleAxisName": "B", "XLIGHT Emission Wheel-Label": "585/11(T)", "640 Shutter-Name": "AnalogIO", "640 AO-Sequenceable": "1", "520 Shutter-Demo": "No", "528 Shutter-Demo": "No", "470-Name": "DA Shutter", "520-DA Device": "520 Shutter", "XYStage-Description": "Ludl XY stage driver adapter", "445 Shutter-MinVolts": "0.0000", "528 AO-TriggerSampleRate": "100000", "405 AO-MinVolts": "0.0000", "405 Shutter-TriggerSequenceLength": "1000", "Height": 1200, "555 AO-Block voltage": "false", "640 Shutter-Volts": "0.0000", "PixelType": "GRAY16", "445 Shutter-Block voltage": "false", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "520 Shutter-TriggerSequenceLength": "1000", "COM9-Name": "COM9", "Core-SLM": "", "555 AO-Volts": "4.5000", "555 Shutter-Block voltage": "false", "XYStage-Speed": "1800.0000", "520 AO-MaxVolts": "5.0000", "528 Shutter-SupportsTriggering": "0", "COM1-Description": "Serial port driver (boost:asio)", "PRIME-MetadataEnabled": "No", "520 Shutter-MinVolts": "0.0000", "470 Shutter-Sequenceable": "1", "PRIME-PreampDelay": "0", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-MinVolts": "0.0000", "ChannelIndex": 1, "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Core-AutoShutter": "1", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "Exposure-ms": 160, "PRIME-PP 2 ENABLED": "Yes", "640 Shutter-TriggerSampleRate": "100000", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "470 Shutter-IOChannel": "Dev1/ao10", "405 AO-Demo": "No", "PixelSizeUm": 1.1, "470 AO-Demo": "No", "470 Shutter-MaxVolts": "5.0000", "Vincent-D3-Name": "Vincent-D3", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FileName": "img_000000000_Il-T_000.tif", "LudlController-Reset": "Operate", "PRIME-Exposure": "160.00", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "445-Name": "DA Shutter", "555 Shutter-Name": "AnalogIO", "520 Shutter-TriggerSampleRate": "100000", "445 Shutter-IOChannel": "Dev1/ao9", "PRIME-TransposeCorrection": "0", "XYStage-StepSize": "0.1000", "445-Description": "DA device that is used as a shutter", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 AO-IOChannel": "Dev1/ao7", "520 Shutter-Name": "AnalogIO", "COM3-DataBits": "8", "640 AO-Volts": "0.0000", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "555 Shutter-IOChannel": "Dev1/ao13", "WaitInterval": null, "520 Shutter-Sequenceable": "1", "YPositionUm": 545.6, "445-State": "0", "445-DA Device": "445 Shutter", "PRIME-CircularBufferAutoSize": "ON", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "555 AO-TriggerSampleRate": "100000", "445 Shutter-Volts": "0.0000", "XLIGHT Hub-Port": "COM3", "Core-AutoFocus": "", "COM4-Handshaking": "Off", "528 Shutter-MinVolts": "0.0000", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "445 Shutter-Name": "AnalogIO", "528 Shutter-Sequenceable": "1", "XYStage-TransposeMirrorX": "0", "XYStage-TransposeMirrorY": "0", "PRIME-ReadoutRate": "100MHz 16bit", "XYStage-Name": "Stage", "640 AO-SupportsTriggering": "0", "640-Name": "DA Shutter", "640 Shutter-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "640 AO-Block voltage": "false", "445 Shutter-Sequenceable": "1", "470 AO-MaxVolts": "5.0000", "COM4-BaudRate": "9600", "470-Description": "DA device that is used as a shutter", "520 Shutter-Description": "NI DAC", "PRIME-CCDTemperature": "-14.8500", "Time": "2019-12-19 09:59:28 -0500", "640 AO-TriggerSequenceLength": "1000", "470 AO-Name": "AnalogIO", "XLIGHT Disk slider-Label": "Disk Pos 40um", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "Binning": "1x1", "TE2000-ModelType": "1", "COM3-AnswerTimeout": "500.0000", "555 Shutter-SupportsTriggering": "0", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "TE2000-Port": "COM4", "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Description": "NI DAC", "COM4-AnswerTimeout": "500.0000", "COM1-DelayBetweenCharsMs": "0.0000", "FilterBlock-Label": "Confocal", "445 AO-Name": "AnalogIO", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-Timing-ReadoutTimeNs": "24672000.0000", "528 AO-Demo": "No", "520 AO-Sequenceable": "1", "555-Description": "DA device that is used as a shutter", "405-DA Device": "405 Shutter", "405 AO-TriggerSequenceLength": "1000", "640 AO-MinVolts": "0.0000", "640 Shutter-Block voltage": "false", "FrameIndex": 0, "PRIME-Port": "CMOS", "555 AO-MinVolts": "0.0000", "COM3-Description": "Serial port driver (boost:asio)", "528 AO-Description": "NI DAC", "555 AO-IOChannel": "Dev1/ao6", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "555 AO-TriggerInputLine": "/Dev1/PFI0", "PRIME-ActualInterval-ms": "390.5200", "LudlController-Port": "COM9", "445 Shutter-Demo": "No", "PRIME-Trigger-Expose Out-Mux": "1", "SliceIndex": 0, "PRIME-CCDTemperatureSetPoint": "-15.0000", "405 Shutter-MinVolts": "0.0000", "PRIME-ExposeOutMode": "First Row", "AxisPositions": null, "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Volts": "5.0000", "528-Name": "DA Shutter", "555-State": "1", "XLIGHT Dichroic-Label": "QUAD", "445 AO-Sequenceable": "1", "405-Name": "DA Shutter", "405 AO-IOChannel": "Dev1/ao0", "Core-Camera": "PRIME", "Focus-Name": "Focus", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "405 Shutter-IOChannel": "Dev1/ao8", "640 AO-Demo": "No", "640 Shutter-SupportsTriggering": "0", "445 AO-Demo": "No", "555-DA Device": "555 Shutter", "470 Shutter-Description": "NI DAC", "528 AO-Volts": "0.0000", "COM1-DataBits": "8", "Vincent-D3-Address": "x", "520 AO-SupportsTriggering": "0", "405-Description": "DA device that is used as a shutter", "640 Shutter-MinVolts": "0.0000", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "405 AO-Name": "AnalogIO", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "640 AO-TriggerInputLine": "/Dev1/PFI0", "405 AO-TriggerInputLine": "/Dev1/PFI0", "640-Description": "DA device that is used as a shutter", "445 Shutter-TriggerSampleRate": "100000", "470 Shutter-TriggerSequenceLength": "1000", "COM1-StopBits": "1", "520 Shutter-SupportsTriggering": "0", "405-State": "0", "Core-Initialize": "1", "XLIGHT Emission Wheel-State": "3", "520 AO-Description": "NI DAC", "528 AO-TriggerInputLine": "/Dev1/PFI0", "445 Shutter-MaxVolts": "5.0000", "COM9-Handshaking": "Off", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "XLIGHT Spin motor-Label": "On (spinning)", "PRIME-PP 5 ENABLED": "No", "UUID": "c616b071-7014-4dbe-b305-8fb030cea61d", "PRIME-TriggerMode": "Internal Trigger", "COM1-Handshaking": "Off", "PRIME-Timing-PostTriggerDelayNs": "49344000.0000", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "Focus-Position": "17741.5000", "528 Shutter-Block voltage": "false", "Channel": "Il-T", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "555 Shutter-Demo": "No", "405 Shutter-Demo": "No", "528 AO-Name": "AnalogIO", "520-State": "0", "Core-Galvo": "", "405 Shutter-TriggerSampleRate": "100000", "Focus-FocusStepSize_(nm)": "100", "PRIME-PixelType": "16bit", "COM4-DataBits": "8", "470 AO-IOChannel": "Dev1/ao2", "520 AO-MinVolts": "0.0000", "COM9-Parity": "None", "PRIME-CircularBufferEnabled": "ON", "PRIME-PP 6 Reset": "No", "640 AO-Description": "NI DAC", "Source": "PRIME", "445 AO-MinVolts": "0.0000", "PIEZO-Name": "Stage", "445 AO-Description": "NI DAC", "PRIME-CircularBufferFrameCount": "46", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "520 AO-Volts": "0.0000", "528 Shutter-TriggerSampleRate": "100000", "XPositionUm": -1324.9, "OpticalPath-State": "4", "PRIME-PP 3 THRESHOLD": "80", "PRIME-PVCAM Version": "3.7.1", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Gain": "1-HDR", "640 Shutter-TriggerSequenceLength": "1000", "528 AO-SupportsTriggering": "0", "445 AO-TriggerInputLine": "/Dev1/PFI0", "OpticalPath-Name": "OpticalPath", "PRIME-PP 4 ENABLED": "Yes", "PRIME-PVCAM Adapter Version": "1.3.29", "COM3-Name": "COM3", "405 AO-MaxVolts": "5.0000", "520 Shutter-Block voltage": "false", "PRIME-Timing-ClearingTimeNs": "49344000.0000", "Core-Focus": "PIEZO", "528 AO-Block voltage": "false", "TE2000-Description": "Nikon TE2000 hub", "528 AO-MinVolts": "0.0000", "COM4-Parity": "None", "Summary": { "Slices": 11, "ChContrastMin": [ 0, 0, 0, 0, 0, 0 ], "PixelAspect": 1, "SlicesFirst": true, "Interval_ms": 0, "Time": "2019-12-19 09:59:21 -0500", "Prefix": "seq_13", "InitialPositionList": [ { "Label": "Pos0", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.21], "Focus": [17741.5], "XYStage": [ 44542.200000000004, 31636.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos1", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.22], "Focus": [17741.4], "XYStage": [ 43801.100000000006, 31637.9 ] }, "GridColumnIndex": 0 }, { "Label": "Pos2", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.5], "XYStage": [ 38561.3, 32040.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos3", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.185], "Focus": [17741.3], "XYStage": [ 37698.4, 32041.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos4", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.16], "Focus": [17741.5], "XYStage": [ 32375.4, 31758.9 ] }, "GridColumnIndex": 0 }, { "Label": "Pos5", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.17000000000002], "Focus": [17741.3], "XYStage": [ 31770.7, 31758.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos6", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.4], "XYStage": [ 26536.800000000003, 31068.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos7", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.205], "Focus": [17741.4], "XYStage": [ 25420.2, 31068.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos8", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.85], "Focus": [17741.3], "XYStage": [ 21059, 31508 ] }, "GridColumnIndex": 0 }, { "Label": "Pos9", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.82], "Focus": [17741.3], "XYStage": [ 19731.9, 31506.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos10", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.79], "Focus": [17741.4], "XYStage": [ 14528.5, 32007.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos11", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.83], "Focus": [17741.2], "XYStage": [ 13830.300000000001, 32009.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos12", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.79], "Focus": [17741.3], "XYStage": [ 8775.300000000001, 32017.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos13", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.815], "Focus": [17741.3], "XYStage": [ 8109.200000000001, 32008.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos14", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.805], "Focus": [17741.3], "XYStage": [ 3483.3, 31908.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos15", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.82], "Focus": [17741.2], "XYStage": [ 2686.8, 31903.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos16", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [211.48000000000002], "Focus": [17741.1], "XYStage": [ -1978.9, 31973.600000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos17", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [211.51500000000001], "Focus": [17741.1], "XYStage": [ -2957.8, 31982.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos18", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.195], "Focus": [17741.5], "XYStage": [ 47747.4, -576.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos19", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.23000000000002], "Focus": [17741.6], "XYStage": [ 47053.9, -576.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos20", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.205], "Focus": [17741.4], "XYStage": [ 41871.200000000004, -479.8 ] }, "GridColumnIndex": 0 }, { "Label": "Pos21", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.4], "XYStage": [ 40882.600000000006, -392 ] }, "GridColumnIndex": 0 }, { "Label": "Pos22", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.24], "Focus": [17741.4], "XYStage": [ 35905.6, -135.3 ] }, "GridColumnIndex": 0 }, { "Label": "Pos23", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.195], "Focus": [17741.5], "XYStage": [ 34990.9, -132.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos24", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.46], "Focus": [17741.4], "XYStage": [ 29678.100000000002, -958.1 ] }, "GridColumnIndex": 0 }, { "Label": "Pos25", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.44], "Focus": [17741.5], "XYStage": [ 28983.600000000002, -939.4000000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos26", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.475], "Focus": [17741.5], "XYStage": [ 23019.7, -122 ] }, "GridColumnIndex": 0 }, { "Label": "Pos27", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.44], "Focus": [17741.7], "XYStage": [ 22428, -116.80000000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos28", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.46], "Focus": [17741.7], "XYStage": [ 16364.900000000001, -181.10000000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos29", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.48000000000002], "Focus": [17741.5], "XYStage": [ 15712.1, -180 ] }, "GridColumnIndex": 0 }, { "Label": "Pos30", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.45000000000002], "Focus": [17741.7], "XYStage": [ 10346.1, 77.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos31", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.49], "Focus": [17741.4], "XYStage": [ 9755.5, 85.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos32", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.475], "Focus": [17741.5], "XYStage": [ 4775.5, 293.90000000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos33", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.445], "Focus": [17741.4], "XYStage": [ 3954.9, 293.90000000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos34", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.455], "Focus": [17741.4], "XYStage": [ -1324.9, 545.6 ] }, "GridColumnIndex": 0 }, { "Label": "Pos35", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.445], "Focus": [17741.3], "XYStage": [ -2097, 576.6 ] }, "GridColumnIndex": 0 } ], "z-step_um": 10, "GridRow": 0, "BitDepth": 16, "Depth": 2, "Channels": 6, "Source": "Micro-Manager", "ChColors": [ -16711681, -256, -65281, -1, -1, -16776961 ], "MetadataVersion": 10, "KeepShutterOpenChannels": true, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536, 65536 ], "CustomIntervals_ms": [], "Height": 1200, "MicroManagerVersion": "1.4.23 20180219", "GridColumn": 0, "UUID": "18808e7c-f2e6-47b3-90d8-1d779ce87dbd", "PixelType": "GRAY16", "Width": 1200, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\Yan\\20191218-YC113-S7S8-miseq", "Frames": 1, "Comment": "", "UserName": "Nikon", "PixelSize_um": 1.1, "ROI": [ 0, 0, 1200, 1200 ], "Date": "2019-12-19", "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC", "P-DAPI-high" ], "KeepShutterOpenSlices": true, "IJType": 1, "ComputerName": "ZadorConfocal2", "Positions": 36, "PositionIndex": 34, "CameraTimeout": 20000, "TimeFirst": false }, "640 AO-Name": "AnalogIO", "Vincent-D3-Command": "Close" }, "FrameKey-0-1-1": { "COM4-StopBits": "1", "528 AO-MaxVolts": "5.0000", "XLIGHT Excitation Wheel-State": "1", "470 Shutter-Name": "AnalogIO", "Core-Shutter": "555", "555 AO-Demo": "No", "XLIGHT Dichroic-State": "2", "XYStage-StepSize-Y": "0.1000", "ZPositionUm": 178.455, "XYStage-StepSize-X": "0.1000", "PRIME-FirmwareVersion": "20.28", "PRIME-ClearCycles": "2", "520-Name": "DA Shutter", "405 Shutter-Name": "AnalogIO", "PIEZO-StepSize": "0.0050", "PRIME-ClearMode": "Pre-Exposure", "470 AO-MinVolts": "0.0000", "405 AO-SupportsTriggering": "1", "CameraChannelIndex": 0, "XYStage-StartSpeed": "500.0000", "COM9-StopBits": "2", "405 AO-Description": "NI DAC", "COM4-DelayBetweenCharsMs": "0.0000", "445 AO-Block voltage": "false", "520 AO-TriggerInputLine": "/Dev1/PFI0", "555 AO-Description": "NI DAC", "520 AO-TriggerSampleRate": "100000", "COM4-Verbose": "1", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM4-Name": "COM4", "405 Shutter-Volts": "0.0000", "Vincent-D3-Port": "COM1", "PRIME-PP 4 MIN ADU AFFECTED": "200", "470 Shutter-MinVolts": "0.0000", "PRIME-PP 3 ENABLED": "Yes", "470 AO-Description": "NI DAC", "PRIME-Description": "PVCAM API device adapter", "520 Shutter-MaxVolts": "5.0000", "520 Shutter-Volts": "0.0000", "528 AO-Sequenceable": "1", "PRIME-Binning": "1x1", "555 Shutter-MaxVolts": "5.0000", "528 Shutter-TriggerSequenceLength": "1000", "405 Shutter-Block voltage": "false", "XLIGHT Spin motor-State": "1", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "Vincent-D3-Time to open (ms)": "35.0000", "Vincent-D3-Time to close (ms)": "35.0000", "445 AO-TriggerSampleRate": "100000", "640 Shutter-IOChannel": "Dev1/ao14", "555 AO-Sequenceable": "1", "520 AO-Name": "AnalogIO", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "470 AO-Block voltage": "false", "COM1-Verbose": "1", "COM9-AnswerTimeout": "10000.0000", "528 Shutter-Name": "AnalogIO", "PRIME-Trigger Timeout (secs)": "10", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "NextFrame": 0, "470 AO-TriggerSequenceLength": "1000", "PRIME-TransposeXY": "0", "445 AO-TriggerSequenceLength": "1000", "COM1-Name": "COM1", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "COM9-DataBits": "8", "COM1-AnswerTimeout": "500.0000", "Camera": "", "TE2000-Version": "V2.50-V2.50-V2.10", "528 Shutter-Volts": "0.0000", "XLIGHT Hub-Name": "XLIGHT Hub", "PIEZO-Description": "Ludl stage driver adapter", "PositionName": "Pos34", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSampleRate": "100000", "PRIME-FanSpeedSetpoint": "High", "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "405 AO-TriggerSampleRate": "100000", "470 Shutter-Block voltage": "false", "470-State": "0", "COM3-Parity": "None", "405 AO-Volts": "0.0000", "PRIME-TransposeMirrorY": "0", "PRIME-ChipName": "GS144BSI", "470 AO-Volts": "0.0000", "PRIME-TransposeMirrorX": "0", "445 Shutter-SupportsTriggering": "0", "470 Shutter-SupportsTriggering": "0", "XLIGHT Hub-Description": "XLIGHT hub", "PRIME-PreampOffLimit": "10000", "470 AO-Sequenceable": "1", "528 Shutter-MaxVolts": "5.0000", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "Core-XYStage": "XYStage", "470 AO-TriggerSampleRate": "100000", "PRIME-Timing-PreTriggerDelayNs": "24672000.0000", "528-DA Device": "528 Shutter", "Focus-Description": "Nikon TE2000 focus adapter", "FilterBlock-State": "5", "520 AO-Demo": "No", "COM3-DelayBetweenCharsMs": "0.0000", "470 Shutter-Volts": "0.0000", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "PRIME-PP 2 THRESHOLD": "125", "PRIME-PP 4 THRESHOLD": "75", "XYStage-Acceleration": "4.0000", "470 Shutter-Demo": "No", "PRIME-Name": "PMPCIECam00", "640-DA Device": "640 Shutter", "405 AO-Block voltage": "false", "ElapsedTime-ms": 1398493, "PRIME-Offset": "100", "445 AO-IOChannel": "Dev1/ao1", "640 AO-MaxVolts": "5.0000", "PositionIndex": 34, "PRIME-PP 1 ENABLED": "Yes", "COM1-BaudRate": "9600", "640-State": "0", "COM3-Verbose": "1", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "LudlController-TransmissionDelay": "0", "PRIME-FullWellCapacity": "62000", "PRIME-SMARTStreamingEnabled": "No", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "640 Shutter-Description": "NI DAC", "528-Description": "DA device that is used as a shutter", "COM4-Description": "Serial port driver (boost:asio)", "405 Shutter-Description": "NI DAC", "TE2000-Name": "TE2000", "528 Shutter-Description": "NI DAC", "COM9-Description": "Serial port driver (boost:asio)", "LudlController-Name": "LudlController", "445 Shutter-TriggerSequenceLength": "1000", "PRIME-SerialNumber": "A18B203008", "445 AO-Volts": "0.0000", "PRIME-X-dimension": "1200", "PRIME-ShutterCloseDelay": "0", "XLIGHT Disk slider-State": "2", "Position": "Default", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 AO-IOChannel": "Dev1/ao5", "520 AO-IOChannel": "Dev1/ao4", "528 AO-TriggerSequenceLength": "1000", "405 Shutter-MaxVolts": "5.0000", "470-DA Device": "470 Shutter", "PRIME-FTCapable": "No", "Core-ChannelGroup": "Channels", "555 AO-SupportsTriggering": "0", "PRIME-Y-dimension": "1200", "Frame": 0, "555 Shutter-Sequenceable": "1", "PRIME-Timing-ExposureTimeNs": "499998000.0000", "Core-ImageProcessor": "", "528-State": "0", "520 AO-Block voltage": "false", "XLIGHT Excitation Wheel-Label": "QUAD", "640 Shutter-Demo": "No", "COM3-StopBits": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "520 Shutter-IOChannel": "Dev1/ao11", "Width": 1200, "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-SupportsTriggering": "0", "640 AO-TriggerSampleRate": "100000", "520-Description": "DA device that is used as a shutter", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "405 Shutter-Sequenceable": "1", "405 Shutter-SupportsTriggering": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-TriggerSampleRate": "100000", "640 Shutter-MaxVolts": "5.0000", "555 AO-MaxVolts": "5.0000", "520 AO-TriggerSequenceLength": "1000", "COM3-BaudRate": "9600", "COM9-BaudRate": "9600", "Vincent-D3-Channel #": "Ch. #1", "PRIME-PMode": "Normal", "OpticalPath-Label": "Left(100)", "BitDepth": 16, "555 AO-TriggerSequenceLength": "1000", "445 Shutter-Description": "NI DAC", "405 AO-Sequenceable": "1", "PRIME-AcquisitionMethod": "Callbacks", "555 AO-Name": "AnalogIO", "PRIME-ShutterMode": "Pre-Exposure", "COM3-Handshaking": "Off", "445 AO-SupportsTriggering": "0", "ROI": "0-0-1200-1200", "COM9-Verbose": "1", "PRIME-Color": "OFF", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "PRIME-ShutterOpenDelay": "0", "COM1-Parity": "None", "Slice": 1, "SlicePosition": -40, "COM9-DelayBetweenCharsMs": "0.0000", "555-Name": "DA Shutter", "PIEZO-LudlSingleAxisName": "B", "XLIGHT Emission Wheel-Label": "585/11(T)", "640 Shutter-Name": "AnalogIO", "640 AO-Sequenceable": "1", "520 Shutter-Demo": "No", "528 Shutter-Demo": "No", "470-Name": "DA Shutter", "520-DA Device": "520 Shutter", "XYStage-Description": "Ludl XY stage driver adapter", "445 Shutter-MinVolts": "0.0000", "528 AO-TriggerSampleRate": "100000", "405 AO-MinVolts": "0.0000", "405 Shutter-TriggerSequenceLength": "1000", "Height": 1200, "555 AO-Block voltage": "false", "640 Shutter-Volts": "0.0000", "PixelType": "GRAY16", "445 Shutter-Block voltage": "false", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "520 Shutter-TriggerSequenceLength": "1000", "COM9-Name": "COM9", "Core-SLM": "", "555 AO-Volts": "4.5000", "555 Shutter-Block voltage": "false", "XYStage-Speed": "1800.0000", "520 AO-MaxVolts": "5.0000", "528 Shutter-SupportsTriggering": "0", "COM1-Description": "Serial port driver (boost:asio)", "PRIME-MetadataEnabled": "No", "520 Shutter-MinVolts": "0.0000", "470 Shutter-Sequenceable": "1", "PRIME-PreampDelay": "0", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-MinVolts": "0.0000", "ChannelIndex": 1, "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Core-AutoShutter": "1", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "Exposure-ms": 160, "PRIME-PP 2 ENABLED": "Yes", "640 Shutter-TriggerSampleRate": "100000", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "470 Shutter-IOChannel": "Dev1/ao10", "405 AO-Demo": "No", "PixelSizeUm": 1.1, "470 AO-Demo": "No", "470 Shutter-MaxVolts": "5.0000", "Vincent-D3-Name": "Vincent-D3", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FileName": "img_000000000_Il-T_001.tif", "LudlController-Reset": "Operate", "PRIME-Exposure": "160.00", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "445-Name": "DA Shutter", "555 Shutter-Name": "AnalogIO", "520 Shutter-TriggerSampleRate": "100000", "445 Shutter-IOChannel": "Dev1/ao9", "PRIME-TransposeCorrection": "0", "XYStage-StepSize": "0.1000", "445-Description": "DA device that is used as a shutter", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 AO-IOChannel": "Dev1/ao7", "520 Shutter-Name": "AnalogIO", "COM3-DataBits": "8", "640 AO-Volts": "0.0000", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "555 Shutter-IOChannel": "Dev1/ao13", "WaitInterval": null, "520 Shutter-Sequenceable": "1", "YPositionUm": 545.6, "445-State": "0", "445-DA Device": "445 Shutter", "PRIME-CircularBufferAutoSize": "ON", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "555 AO-TriggerSampleRate": "100000", "445 Shutter-Volts": "0.0000", "XLIGHT Hub-Port": "COM3", "Core-AutoFocus": "", "COM4-Handshaking": "Off", "528 Shutter-MinVolts": "0.0000", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "445 Shutter-Name": "AnalogIO", "528 Shutter-Sequenceable": "1", "XYStage-TransposeMirrorX": "0", "XYStage-TransposeMirrorY": "0", "PRIME-ReadoutRate": "100MHz 16bit", "XYStage-Name": "Stage", "640 AO-SupportsTriggering": "0", "640-Name": "DA Shutter", "640 Shutter-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "640 AO-Block voltage": "false", "445 Shutter-Sequenceable": "1", "470 AO-MaxVolts": "5.0000", "COM4-BaudRate": "9600", "470-Description": "DA device that is used as a shutter", "520 Shutter-Description": "NI DAC", "PRIME-CCDTemperature": "-14.8500", "Time": "2019-12-19 09:59:28 -0500", "640 AO-TriggerSequenceLength": "1000", "470 AO-Name": "AnalogIO", "XLIGHT Disk slider-Label": "Disk Pos 40um", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "Binning": "1x1", "TE2000-ModelType": "1", "COM3-AnswerTimeout": "500.0000", "555 Shutter-SupportsTriggering": "0", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "TE2000-Port": "COM4", "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Description": "NI DAC", "COM4-AnswerTimeout": "500.0000", "COM1-DelayBetweenCharsMs": "0.0000", "FilterBlock-Label": "Confocal", "445 AO-Name": "AnalogIO", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-Timing-ReadoutTimeNs": "24672000.0000", "528 AO-Demo": "No", "520 AO-Sequenceable": "1", "555-Description": "DA device that is used as a shutter", "405-DA Device": "405 Shutter", "405 AO-TriggerSequenceLength": "1000", "640 AO-MinVolts": "0.0000", "640 Shutter-Block voltage": "false", "FrameIndex": 0, "PRIME-Port": "CMOS", "555 AO-MinVolts": "0.0000", "COM3-Description": "Serial port driver (boost:asio)", "528 AO-Description": "NI DAC", "555 AO-IOChannel": "Dev1/ao6", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "555 AO-TriggerInputLine": "/Dev1/PFI0", "PRIME-ActualInterval-ms": "390.5200", "LudlController-Port": "COM9", "445 Shutter-Demo": "No", "PRIME-Trigger-Expose Out-Mux": "1", "SliceIndex": 1, "PRIME-CCDTemperatureSetPoint": "-15.0000", "405 Shutter-MinVolts": "0.0000", "PRIME-ExposeOutMode": "First Row", "AxisPositions": null, "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Volts": "5.0000", "528-Name": "DA Shutter", "555-State": "1", "XLIGHT Dichroic-Label": "QUAD", "445 AO-Sequenceable": "1", "405-Name": "DA Shutter", "405 AO-IOChannel": "Dev1/ao0", "Core-Camera": "PRIME", "Focus-Name": "Focus", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "405 Shutter-IOChannel": "Dev1/ao8", "640 AO-Demo": "No", "640 Shutter-SupportsTriggering": "0", "445 AO-Demo": "No", "555-DA Device": "555 Shutter", "470 Shutter-Description": "NI DAC", "528 AO-Volts": "0.0000", "COM1-DataBits": "8", "Vincent-D3-Address": "x", "520 AO-SupportsTriggering": "0", "405-Description": "DA device that is used as a shutter", "640 Shutter-MinVolts": "0.0000", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "405 AO-Name": "AnalogIO", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "640 AO-TriggerInputLine": "/Dev1/PFI0", "405 AO-TriggerInputLine": "/Dev1/PFI0", "640-Description": "DA device that is used as a shutter", "445 Shutter-TriggerSampleRate": "100000", "470 Shutter-TriggerSequenceLength": "1000", "COM1-StopBits": "1", "520 Shutter-SupportsTriggering": "0", "405-State": "0", "Core-Initialize": "1", "XLIGHT Emission Wheel-State": "3", "520 AO-Description": "NI DAC", "528 AO-TriggerInputLine": "/Dev1/PFI0", "445 Shutter-MaxVolts": "5.0000", "COM9-Handshaking": "Off", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "XLIGHT Spin motor-Label": "On (spinning)", "PRIME-PP 5 ENABLED": "No", "UUID": "11856f9d-88ef-45bd-8e4f-b19ee4e34abe", "PRIME-TriggerMode": "Internal Trigger", "COM1-Handshaking": "Off", "PRIME-Timing-PostTriggerDelayNs": "49344000.0000", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "Focus-Position": "17741.5000", "528 Shutter-Block voltage": "false", "Channel": "Il-T", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "555 Shutter-Demo": "No", "405 Shutter-Demo": "No", "528 AO-Name": "AnalogIO", "520-State": "0", "Core-Galvo": "", "405 Shutter-TriggerSampleRate": "100000", "Focus-FocusStepSize_(nm)": "100", "PRIME-PixelType": "16bit", "COM4-DataBits": "8", "470 AO-IOChannel": "Dev1/ao2", "520 AO-MinVolts": "0.0000", "COM9-Parity": "None", "PRIME-CircularBufferEnabled": "ON", "PRIME-PP 6 Reset": "No", "640 AO-Description": "NI DAC", "Source": "PRIME", "445 AO-MinVolts": "0.0000", "PIEZO-Name": "Stage", "445 AO-Description": "NI DAC", "PRIME-CircularBufferFrameCount": "46", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "520 AO-Volts": "0.0000", "528 Shutter-TriggerSampleRate": "100000", "XPositionUm": -1324.9, "OpticalPath-State": "4", "PRIME-PP 3 THRESHOLD": "80", "PRIME-PVCAM Version": "3.7.1", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Gain": "1-HDR", "640 Shutter-TriggerSequenceLength": "1000", "528 AO-SupportsTriggering": "0", "445 AO-TriggerInputLine": "/Dev1/PFI0", "OpticalPath-Name": "OpticalPath", "PRIME-PP 4 ENABLED": "Yes", "PRIME-PVCAM Adapter Version": "1.3.29", "COM3-Name": "COM3", "405 AO-MaxVolts": "5.0000", "520 Shutter-Block voltage": "false", "PRIME-Timing-ClearingTimeNs": "49344000.0000", "Core-Focus": "PIEZO", "528 AO-Block voltage": "false", "TE2000-Description": "Nikon TE2000 hub", "528 AO-MinVolts": "0.0000", "COM4-Parity": "None", "Summary": { "Slices": 11, "ChContrastMin": [ 0, 0, 0, 0, 0, 0 ], "PixelAspect": 1, "SlicesFirst": true, "Interval_ms": 0, "Time": "2019-12-19 09:59:21 -0500", "Prefix": "seq_13", "InitialPositionList": [ { "Label": "Pos0", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.21], "Focus": [17741.5], "XYStage": [ 44542.200000000004, 31636.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos1", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.22], "Focus": [17741.4], "XYStage": [ 43801.100000000006, 31637.9 ] }, "GridColumnIndex": 0 }, { "Label": "Pos2", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.5], "XYStage": [ 38561.3, 32040.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos3", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.185], "Focus": [17741.3], "XYStage": [ 37698.4, 32041.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos4", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.16], "Focus": [17741.5], "XYStage": [ 32375.4, 31758.9 ] }, "GridColumnIndex": 0 }, { "Label": "Pos5", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.17000000000002], "Focus": [17741.3], "XYStage": [ 31770.7, 31758.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos6", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.4], "XYStage": [ 26536.800000000003, 31068.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos7", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.205], "Focus": [17741.4], "XYStage": [ 25420.2, 31068.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos8", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.85], "Focus": [17741.3], "XYStage": [ 21059, 31508 ] }, "GridColumnIndex": 0 }, { "Label": "Pos9", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.82], "Focus": [17741.3], "XYStage": [ 19731.9, 31506.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos10", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.79], "Focus": [17741.4], "XYStage": [ 14528.5, 32007.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos11", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.83], "Focus": [17741.2], "XYStage": [ 13830.300000000001, 32009.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos12", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.79], "Focus": [17741.3], "XYStage": [ 8775.300000000001, 32017.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos13", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.815], "Focus": [17741.3], "XYStage": [ 8109.200000000001, 32008.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos14", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.805], "Focus": [17741.3], "XYStage": [ 3483.3, 31908.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos15", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.82], "Focus": [17741.2], "XYStage": [ 2686.8, 31903.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos16", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [211.48000000000002], "Focus": [17741.1], "XYStage": [ -1978.9, 31973.600000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos17", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [211.51500000000001], "Focus": [17741.1], "XYStage": [ -2957.8, 31982.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos18", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.195], "Focus": [17741.5], "XYStage": [ 47747.4, -576.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos19", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.23000000000002], "Focus": [17741.6], "XYStage": [ 47053.9, -576.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos20", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.205], "Focus": [17741.4], "XYStage": [ 41871.200000000004, -479.8 ] }, "GridColumnIndex": 0 }, { "Label": "Pos21", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.4], "XYStage": [ 40882.600000000006, -392 ] }, "GridColumnIndex": 0 }, { "Label": "Pos22", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.24], "Focus": [17741.4], "XYStage": [ 35905.6, -135.3 ] }, "GridColumnIndex": 0 }, { "Label": "Pos23", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.195], "Focus": [17741.5], "XYStage": [ 34990.9, -132.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos24", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.46], "Focus": [17741.4], "XYStage": [ 29678.100000000002, -958.1 ] }, "GridColumnIndex": 0 }, { "Label": "Pos25", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.44], "Focus": [17741.5], "XYStage": [ 28983.600000000002, -939.4000000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos26", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.475], "Focus": [17741.5], "XYStage": [ 23019.7, -122 ] }, "GridColumnIndex": 0 }, { "Label": "Pos27", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.44], "Focus": [17741.7], "XYStage": [ 22428, -116.80000000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos28", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.46], "Focus": [17741.7], "XYStage": [ 16364.900000000001, -181.10000000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos29", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.48000000000002], "Focus": [17741.5], "XYStage": [ 15712.1, -180 ] }, "GridColumnIndex": 0 }, { "Label": "Pos30", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.45000000000002], "Focus": [17741.7], "XYStage": [ 10346.1, 77.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos31", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.49], "Focus": [17741.4], "XYStage": [ 9755.5, 85.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos32", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.475], "Focus": [17741.5], "XYStage": [ 4775.5, 293.90000000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos33", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.445], "Focus": [17741.4], "XYStage": [ 3954.9, 293.90000000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos34", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.455], "Focus": [17741.4], "XYStage": [ -1324.9, 545.6 ] }, "GridColumnIndex": 0 }, { "Label": "Pos35", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.445], "Focus": [17741.3], "XYStage": [ -2097, 576.6 ] }, "GridColumnIndex": 0 } ], "z-step_um": 10, "GridRow": 0, "BitDepth": 16, "Depth": 2, "Channels": 6, "Source": "Micro-Manager", "ChColors": [ -16711681, -256, -65281, -1, -1, -16776961 ], "MetadataVersion": 10, "KeepShutterOpenChannels": true, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536, 65536 ], "CustomIntervals_ms": [], "Height": 1200, "MicroManagerVersion": "1.4.23 20180219", "GridColumn": 0, "UUID": "18808e7c-f2e6-47b3-90d8-1d779ce87dbd", "PixelType": "GRAY16", "Width": 1200, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\Yan\\20191218-YC113-S7S8-miseq", "Frames": 1, "Comment": "", "UserName": "Nikon", "PixelSize_um": 1.1, "ROI": [ 0, 0, 1200, 1200 ], "Date": "2019-12-19", "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC", "P-DAPI-high" ], "KeepShutterOpenSlices": true, "IJType": 1, "ComputerName": "ZadorConfocal2", "Positions": 36, "PositionIndex": 34, "CameraTimeout": 20000, "TimeFirst": false }, "640 AO-Name": "AnalogIO", "Vincent-D3-Command": "Close" }, "FrameKey-0-1-2": { "COM4-StopBits": "1", "528 AO-MaxVolts": "5.0000", "XLIGHT Excitation Wheel-State": "1", "470 Shutter-Name": "AnalogIO", "Core-Shutter": "555", "555 AO-Demo": "No", "XLIGHT Dichroic-State": "2", "XYStage-StepSize-Y": "0.1000", "ZPositionUm": 188.455, "XYStage-StepSize-X": "0.1000", "PRIME-FirmwareVersion": "20.28", "PRIME-ClearCycles": "2", "520-Name": "DA Shutter", "405 Shutter-Name": "AnalogIO", "PIEZO-StepSize": "0.0050", "PRIME-ClearMode": "Pre-Exposure", "470 AO-MinVolts": "0.0000", "405 AO-SupportsTriggering": "1", "CameraChannelIndex": 0, "XYStage-StartSpeed": "500.0000", "COM9-StopBits": "2", "405 AO-Description": "NI DAC", "COM4-DelayBetweenCharsMs": "0.0000", "445 AO-Block voltage": "false", "520 AO-TriggerInputLine": "/Dev1/PFI0", "555 AO-Description": "NI DAC", "520 AO-TriggerSampleRate": "100000", "COM4-Verbose": "1", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM4-Name": "COM4", "405 Shutter-Volts": "0.0000", "Vincent-D3-Port": "COM1", "PRIME-PP 4 MIN ADU AFFECTED": "200", "470 Shutter-MinVolts": "0.0000", "PRIME-PP 3 ENABLED": "Yes", "470 AO-Description": "NI DAC", "PRIME-Description": "PVCAM API device adapter", "520 Shutter-MaxVolts": "5.0000", "520 Shutter-Volts": "0.0000", "528 AO-Sequenceable": "1", "PRIME-Binning": "1x1", "555 Shutter-MaxVolts": "5.0000", "528 Shutter-TriggerSequenceLength": "1000", "405 Shutter-Block voltage": "false", "XLIGHT Spin motor-State": "1", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "Vincent-D3-Time to open (ms)": "35.0000", "Vincent-D3-Time to close (ms)": "35.0000", "445 AO-TriggerSampleRate": "100000", "640 Shutter-IOChannel": "Dev1/ao14", "555 AO-Sequenceable": "1", "520 AO-Name": "AnalogIO", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "470 AO-Block voltage": "false", "COM1-Verbose": "1", "COM9-AnswerTimeout": "10000.0000", "528 Shutter-Name": "AnalogIO", "PRIME-Trigger Timeout (secs)": "10", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "NextFrame": 0, "470 AO-TriggerSequenceLength": "1000", "PRIME-TransposeXY": "0", "445 AO-TriggerSequenceLength": "1000", "COM1-Name": "COM1", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "COM9-DataBits": "8", "COM1-AnswerTimeout": "500.0000", "Camera": "", "TE2000-Version": "V2.50-V2.50-V2.10", "528 Shutter-Volts": "0.0000", "XLIGHT Hub-Name": "XLIGHT Hub", "PIEZO-Description": "Ludl stage driver adapter", "PositionName": "Pos34", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSampleRate": "100000", "PRIME-FanSpeedSetpoint": "High", "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "405 AO-TriggerSampleRate": "100000", "470 Shutter-Block voltage": "false", "470-State": "0", "COM3-Parity": "None", "405 AO-Volts": "0.0000", "PRIME-TransposeMirrorY": "0", "PRIME-ChipName": "GS144BSI", "470 AO-Volts": "0.0000", "PRIME-TransposeMirrorX": "0", "445 Shutter-SupportsTriggering": "0", "470 Shutter-SupportsTriggering": "0", "XLIGHT Hub-Description": "XLIGHT hub", "PRIME-PreampOffLimit": "10000", "470 AO-Sequenceable": "1", "528 Shutter-MaxVolts": "5.0000", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "Core-XYStage": "XYStage", "470 AO-TriggerSampleRate": "100000", "PRIME-Timing-PreTriggerDelayNs": "24672000.0000", "528-DA Device": "528 Shutter", "Focus-Description": "Nikon TE2000 focus adapter", "FilterBlock-State": "5", "520 AO-Demo": "No", "COM3-DelayBetweenCharsMs": "0.0000", "470 Shutter-Volts": "0.0000", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "PRIME-PP 2 THRESHOLD": "125", "PRIME-PP 4 THRESHOLD": "75", "XYStage-Acceleration": "4.0000", "470 Shutter-Demo": "No", "PRIME-Name": "PMPCIECam00", "640-DA Device": "640 Shutter", "405 AO-Block voltage": "false", "ElapsedTime-ms": 1398939, "PRIME-Offset": "100", "445 AO-IOChannel": "Dev1/ao1", "640 AO-MaxVolts": "5.0000", "PositionIndex": 34, "PRIME-PP 1 ENABLED": "Yes", "COM1-BaudRate": "9600", "640-State": "0", "COM3-Verbose": "1", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "LudlController-TransmissionDelay": "0", "PRIME-FullWellCapacity": "62000", "PRIME-SMARTStreamingEnabled": "No", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "640 Shutter-Description": "NI DAC", "528-Description": "DA device that is used as a shutter", "COM4-Description": "Serial port driver (boost:asio)", "405 Shutter-Description": "NI DAC", "TE2000-Name": "TE2000", "528 Shutter-Description": "NI DAC", "COM9-Description": "Serial port driver (boost:asio)", "LudlController-Name": "LudlController", "445 Shutter-TriggerSequenceLength": "1000", "PRIME-SerialNumber": "A18B203008", "445 AO-Volts": "0.0000", "PRIME-X-dimension": "1200", "PRIME-ShutterCloseDelay": "0", "XLIGHT Disk slider-State": "2", "Position": "Default", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 AO-IOChannel": "Dev1/ao5", "520 AO-IOChannel": "Dev1/ao4", "528 AO-TriggerSequenceLength": "1000", "405 Shutter-MaxVolts": "5.0000", "470-DA Device": "470 Shutter", "PRIME-FTCapable": "No", "Core-ChannelGroup": "Channels", "555 AO-SupportsTriggering": "0", "PRIME-Y-dimension": "1200", "Frame": 0, "555 Shutter-Sequenceable": "1", "PRIME-Timing-ExposureTimeNs": "499998000.0000", "Core-ImageProcessor": "", "528-State": "0", "520 AO-Block voltage": "false", "XLIGHT Excitation Wheel-Label": "QUAD", "640 Shutter-Demo": "No", "COM3-StopBits": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "520 Shutter-IOChannel": "Dev1/ao11", "Width": 1200, "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-SupportsTriggering": "0", "640 AO-TriggerSampleRate": "100000", "520-Description": "DA device that is used as a shutter", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "405 Shutter-Sequenceable": "1", "405 Shutter-SupportsTriggering": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-TriggerSampleRate": "100000", "640 Shutter-MaxVolts": "5.0000", "555 AO-MaxVolts": "5.0000", "520 AO-TriggerSequenceLength": "1000", "COM3-BaudRate": "9600", "COM9-BaudRate": "9600", "Vincent-D3-Channel #": "Ch. #1", "PRIME-PMode": "Normal", "OpticalPath-Label": "Left(100)", "BitDepth": 16, "555 AO-TriggerSequenceLength": "1000", "445 Shutter-Description": "NI DAC", "405 AO-Sequenceable": "1", "PRIME-AcquisitionMethod": "Callbacks", "555 AO-Name": "AnalogIO", "PRIME-ShutterMode": "Pre-Exposure", "COM3-Handshaking": "Off", "445 AO-SupportsTriggering": "0", "ROI": "0-0-1200-1200", "COM9-Verbose": "1", "PRIME-Color": "OFF", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "PRIME-ShutterOpenDelay": "0", "COM1-Parity": "None", "Slice": 2, "SlicePosition": -30, "COM9-DelayBetweenCharsMs": "0.0000", "555-Name": "DA Shutter", "PIEZO-LudlSingleAxisName": "B", "XLIGHT Emission Wheel-Label": "585/11(T)", "640 Shutter-Name": "AnalogIO", "640 AO-Sequenceable": "1", "520 Shutter-Demo": "No", "528 Shutter-Demo": "No", "470-Name": "DA Shutter", "520-DA Device": "520 Shutter", "XYStage-Description": "Ludl XY stage driver adapter", "445 Shutter-MinVolts": "0.0000", "528 AO-TriggerSampleRate": "100000", "405 AO-MinVolts": "0.0000", "405 Shutter-TriggerSequenceLength": "1000", "Height": 1200, "555 AO-Block voltage": "false", "640 Shutter-Volts": "0.0000", "PixelType": "GRAY16", "445 Shutter-Block voltage": "false", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "520 Shutter-TriggerSequenceLength": "1000", "COM9-Name": "COM9", "Core-SLM": "", "555 AO-Volts": "4.5000", "555 Shutter-Block voltage": "false", "XYStage-Speed": "1800.0000", "520 AO-MaxVolts": "5.0000", "528 Shutter-SupportsTriggering": "0", "COM1-Description": "Serial port driver (boost:asio)", "PRIME-MetadataEnabled": "No", "520 Shutter-MinVolts": "0.0000", "470 Shutter-Sequenceable": "1", "PRIME-PreampDelay": "0", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-MinVolts": "0.0000", "ChannelIndex": 1, "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Core-AutoShutter": "1", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "Exposure-ms": 160, "PRIME-PP 2 ENABLED": "Yes", "640 Shutter-TriggerSampleRate": "100000", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "470 Shutter-IOChannel": "Dev1/ao10", "405 AO-Demo": "No", "PixelSizeUm": 1.1, "470 AO-Demo": "No", "470 Shutter-MaxVolts": "5.0000", "Vincent-D3-Name": "Vincent-D3", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FileName": "img_000000000_Il-T_002.tif", "LudlController-Reset": "Operate", "PRIME-Exposure": "160.00", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "445-Name": "DA Shutter", "555 Shutter-Name": "AnalogIO", "520 Shutter-TriggerSampleRate": "100000", "445 Shutter-IOChannel": "Dev1/ao9", "PRIME-TransposeCorrection": "0", "XYStage-StepSize": "0.1000", "445-Description": "DA device that is used as a shutter", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 AO-IOChannel": "Dev1/ao7", "520 Shutter-Name": "AnalogIO", "COM3-DataBits": "8", "640 AO-Volts": "0.0000", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "555 Shutter-IOChannel": "Dev1/ao13", "WaitInterval": null, "520 Shutter-Sequenceable": "1", "YPositionUm": 545.6, "445-State": "0", "445-DA Device": "445 Shutter", "PRIME-CircularBufferAutoSize": "ON", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "555 AO-TriggerSampleRate": "100000", "445 Shutter-Volts": "0.0000", "XLIGHT Hub-Port": "COM3", "Core-AutoFocus": "", "COM4-Handshaking": "Off", "528 Shutter-MinVolts": "0.0000", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "445 Shutter-Name": "AnalogIO", "528 Shutter-Sequenceable": "1", "XYStage-TransposeMirrorX": "0", "XYStage-TransposeMirrorY": "0", "PRIME-ReadoutRate": "100MHz 16bit", "XYStage-Name": "Stage", "640 AO-SupportsTriggering": "0", "640-Name": "DA Shutter", "640 Shutter-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "640 AO-Block voltage": "false", "445 Shutter-Sequenceable": "1", "470 AO-MaxVolts": "5.0000", "COM4-BaudRate": "9600", "470-Description": "DA device that is used as a shutter", "520 Shutter-Description": "NI DAC", "PRIME-CCDTemperature": "-14.8500", "Time": "2019-12-19 09:59:29 -0500", "640 AO-TriggerSequenceLength": "1000", "470 AO-Name": "AnalogIO", "XLIGHT Disk slider-Label": "Disk Pos 40um", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "Binning": "1x1", "TE2000-ModelType": "1", "COM3-AnswerTimeout": "500.0000", "555 Shutter-SupportsTriggering": "0", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "TE2000-Port": "COM4", "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Description": "NI DAC", "COM4-AnswerTimeout": "500.0000", "COM1-DelayBetweenCharsMs": "0.0000", "FilterBlock-Label": "Confocal", "445 AO-Name": "AnalogIO", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-Timing-ReadoutTimeNs": "24672000.0000", "528 AO-Demo": "No", "520 AO-Sequenceable": "1", "555-Description": "DA device that is used as a shutter", "405-DA Device": "405 Shutter", "405 AO-TriggerSequenceLength": "1000", "640 AO-MinVolts": "0.0000", "640 Shutter-Block voltage": "false", "FrameIndex": 0, "PRIME-Port": "CMOS", "555 AO-MinVolts": "0.0000", "COM3-Description": "Serial port driver (boost:asio)", "528 AO-Description": "NI DAC", "555 AO-IOChannel": "Dev1/ao6", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "555 AO-TriggerInputLine": "/Dev1/PFI0", "PRIME-ActualInterval-ms": "390.5200", "LudlController-Port": "COM9", "445 Shutter-Demo": "No", "PRIME-Trigger-Expose Out-Mux": "1", "SliceIndex": 2, "PRIME-CCDTemperatureSetPoint": "-15.0000", "405 Shutter-MinVolts": "0.0000", "PRIME-ExposeOutMode": "First Row", "AxisPositions": null, "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Volts": "5.0000", "528-Name": "DA Shutter", "555-State": "1", "XLIGHT Dichroic-Label": "QUAD", "445 AO-Sequenceable": "1", "405-Name": "DA Shutter", "405 AO-IOChannel": "Dev1/ao0", "Core-Camera": "PRIME", "Focus-Name": "Focus", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "405 Shutter-IOChannel": "Dev1/ao8", "640 AO-Demo": "No", "640 Shutter-SupportsTriggering": "0", "445 AO-Demo": "No", "555-DA Device": "555 Shutter", "470 Shutter-Description": "NI DAC", "528 AO-Volts": "0.0000", "COM1-DataBits": "8", "Vincent-D3-Address": "x", "520 AO-SupportsTriggering": "0", "405-Description": "DA device that is used as a shutter", "640 Shutter-MinVolts": "0.0000", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "405 AO-Name": "AnalogIO", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "640 AO-TriggerInputLine": "/Dev1/PFI0", "405 AO-TriggerInputLine": "/Dev1/PFI0", "640-Description": "DA device that is used as a shutter", "445 Shutter-TriggerSampleRate": "100000", "470 Shutter-TriggerSequenceLength": "1000", "COM1-StopBits": "1", "520 Shutter-SupportsTriggering": "0", "405-State": "0", "Core-Initialize": "1", "XLIGHT Emission Wheel-State": "3", "520 AO-Description": "NI DAC", "528 AO-TriggerInputLine": "/Dev1/PFI0", "445 Shutter-MaxVolts": "5.0000", "COM9-Handshaking": "Off", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "XLIGHT Spin motor-Label": "On (spinning)", "PRIME-PP 5 ENABLED": "No", "UUID": "ffa5a07c-7b05-49fb-a698-2e1fb2bc41bd", "PRIME-TriggerMode": "Internal Trigger", "COM1-Handshaking": "Off", "PRIME-Timing-PostTriggerDelayNs": "49344000.0000", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "Focus-Position": "17741.5000", "528 Shutter-Block voltage": "false", "Channel": "Il-T", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "555 Shutter-Demo": "No", "405 Shutter-Demo": "No", "528 AO-Name": "AnalogIO", "520-State": "0", "Core-Galvo": "", "405 Shutter-TriggerSampleRate": "100000", "Focus-FocusStepSize_(nm)": "100", "PRIME-PixelType": "16bit", "COM4-DataBits": "8", "470 AO-IOChannel": "Dev1/ao2", "520 AO-MinVolts": "0.0000", "COM9-Parity": "None", "PRIME-CircularBufferEnabled": "ON", "PRIME-PP 6 Reset": "No", "640 AO-Description": "NI DAC", "Source": "PRIME", "445 AO-MinVolts": "0.0000", "PIEZO-Name": "Stage", "445 AO-Description": "NI DAC", "PRIME-CircularBufferFrameCount": "46", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "520 AO-Volts": "0.0000", "528 Shutter-TriggerSampleRate": "100000", "XPositionUm": -1324.9, "OpticalPath-State": "4", "PRIME-PP 3 THRESHOLD": "80", "PRIME-PVCAM Version": "3.7.1", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Gain": "1-HDR", "640 Shutter-TriggerSequenceLength": "1000", "528 AO-SupportsTriggering": "0", "445 AO-TriggerInputLine": "/Dev1/PFI0", "OpticalPath-Name": "OpticalPath", "PRIME-PP 4 ENABLED": "Yes", "PRIME-PVCAM Adapter Version": "1.3.29", "COM3-Name": "COM3", "405 AO-MaxVolts": "5.0000", "520 Shutter-Block voltage": "false", "PRIME-Timing-ClearingTimeNs": "49344000.0000", "Core-Focus": "PIEZO", "528 AO-Block voltage": "false", "TE2000-Description": "Nikon TE2000 hub", "528 AO-MinVolts": "0.0000", "COM4-Parity": "None", "Summary": { "Slices": 11, "ChContrastMin": [ 0, 0, 0, 0, 0, 0 ], "PixelAspect": 1, "SlicesFirst": true, "Interval_ms": 0, "Time": "2019-12-19 09:59:21 -0500", "Prefix": "seq_13", "InitialPositionList": [ { "Label": "Pos0", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.21], "Focus": [17741.5], "XYStage": [ 44542.200000000004, 31636.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos1", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.22], "Focus": [17741.4], "XYStage": [ 43801.100000000006, 31637.9 ] }, "GridColumnIndex": 0 }, { "Label": "Pos2", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.5], "XYStage": [ 38561.3, 32040.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos3", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.185], "Focus": [17741.3], "XYStage": [ 37698.4, 32041.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos4", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.16], "Focus": [17741.5], "XYStage": [ 32375.4, 31758.9 ] }, "GridColumnIndex": 0 }, { "Label": "Pos5", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.17000000000002], "Focus": [17741.3], "XYStage": [ 31770.7, 31758.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos6", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.4], "XYStage": [ 26536.800000000003, 31068.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos7", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.205], "Focus": [17741.4], "XYStage": [ 25420.2, 31068.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos8", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.85], "Focus": [17741.3], "XYStage": [ 21059, 31508 ] }, "GridColumnIndex": 0 }, { "Label": "Pos9", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.82], "Focus": [17741.3], "XYStage": [ 19731.9, 31506.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos10", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.79], "Focus": [17741.4], "XYStage": [ 14528.5, 32007.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos11", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.83], "Focus": [17741.2], "XYStage": [ 13830.300000000001, 32009.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos12", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.79], "Focus": [17741.3], "XYStage": [ 8775.300000000001, 32017.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos13", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.815], "Focus": [17741.3], "XYStage": [ 8109.200000000001, 32008.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos14", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.805], "Focus": [17741.3], "XYStage": [ 3483.3, 31908.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos15", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.82], "Focus": [17741.2], "XYStage": [ 2686.8, 31903.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos16", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [211.48000000000002], "Focus": [17741.1], "XYStage": [ -1978.9, 31973.600000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos17", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [211.51500000000001], "Focus": [17741.1], "XYStage": [ -2957.8, 31982.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos18", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.195], "Focus": [17741.5], "XYStage": [ 47747.4, -576.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos19", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.23000000000002], "Focus": [17741.6], "XYStage": [ 47053.9, -576.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos20", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.205], "Focus": [17741.4], "XYStage": [ 41871.200000000004, -479.8 ] }, "GridColumnIndex": 0 }, { "Label": "Pos21", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.4], "XYStage": [ 40882.600000000006, -392 ] }, "GridColumnIndex": 0 }, { "Label": "Pos22", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.24], "Focus": [17741.4], "XYStage": [ 35905.6, -135.3 ] }, "GridColumnIndex": 0 }, { "Label": "Pos23", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.195], "Focus": [17741.5], "XYStage": [ 34990.9, -132.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos24", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.46], "Focus": [17741.4], "XYStage": [ 29678.100000000002, -958.1 ] }, "GridColumnIndex": 0 }, { "Label": "Pos25", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.44], "Focus": [17741.5], "XYStage": [ 28983.600000000002, -939.4000000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos26", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.475], "Focus": [17741.5], "XYStage": [ 23019.7, -122 ] }, "GridColumnIndex": 0 }, { "Label": "Pos27", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.44], "Focus": [17741.7], "XYStage": [ 22428, -116.80000000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos28", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.46], "Focus": [17741.7], "XYStage": [ 16364.900000000001, -181.10000000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos29", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.48000000000002], "Focus": [17741.5], "XYStage": [ 15712.1, -180 ] }, "GridColumnIndex": 0 }, { "Label": "Pos30", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.45000000000002], "Focus": [17741.7], "XYStage": [ 10346.1, 77.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos31", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.49], "Focus": [17741.4], "XYStage": [ 9755.5, 85.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos32", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.475], "Focus": [17741.5], "XYStage": [ 4775.5, 293.90000000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos33", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.445], "Focus": [17741.4], "XYStage": [ 3954.9, 293.90000000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos34", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.455], "Focus": [17741.4], "XYStage": [ -1324.9, 545.6 ] }, "GridColumnIndex": 0 }, { "Label": "Pos35", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.445], "Focus": [17741.3], "XYStage": [ -2097, 576.6 ] }, "GridColumnIndex": 0 } ], "z-step_um": 10, "GridRow": 0, "BitDepth": 16, "Depth": 2, "Channels": 6, "Source": "Micro-Manager", "ChColors": [ -16711681, -256, -65281, -1, -1, -16776961 ], "MetadataVersion": 10, "KeepShutterOpenChannels": true, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536, 65536 ], "CustomIntervals_ms": [], "Height": 1200, "MicroManagerVersion": "1.4.23 20180219", "GridColumn": 0, "UUID": "18808e7c-f2e6-47b3-90d8-1d779ce87dbd", "PixelType": "GRAY16", "Width": 1200, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\Yan\\20191218-YC113-S7S8-miseq", "Frames": 1, "Comment": "", "UserName": "Nikon", "PixelSize_um": 1.1, "ROI": [ 0, 0, 1200, 1200 ], "Date": "2019-12-19", "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC", "P-DAPI-high" ], "KeepShutterOpenSlices": true, "IJType": 1, "ComputerName": "ZadorConfocal2", "Positions": 36, "PositionIndex": 34, "CameraTimeout": 20000, "TimeFirst": false }, "640 AO-Name": "AnalogIO", "Vincent-D3-Command": "Close" }, "FrameKey-0-1-3": { "COM4-StopBits": "1", "528 AO-MaxVolts": "5.0000", "XLIGHT Excitation Wheel-State": "1", "470 Shutter-Name": "AnalogIO", "Core-Shutter": "555", "555 AO-Demo": "No", "XLIGHT Dichroic-State": "2", "XYStage-StepSize-Y": "0.1000", "ZPositionUm": 198.455, "XYStage-StepSize-X": "0.1000", "PRIME-FirmwareVersion": "20.28", "PRIME-ClearCycles": "2", "520-Name": "DA Shutter", "405 Shutter-Name": "AnalogIO", "PIEZO-StepSize": "0.0050", "PRIME-ClearMode": "Pre-Exposure", "470 AO-MinVolts": "0.0000", "405 AO-SupportsTriggering": "1", "CameraChannelIndex": 0, "XYStage-StartSpeed": "500.0000", "COM9-StopBits": "2", "405 AO-Description": "NI DAC", "COM4-DelayBetweenCharsMs": "0.0000", "445 AO-Block voltage": "false", "520 AO-TriggerInputLine": "/Dev1/PFI0", "555 AO-Description": "NI DAC", "520 AO-TriggerSampleRate": "100000", "COM4-Verbose": "1", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM4-Name": "COM4", "405 Shutter-Volts": "0.0000", "Vincent-D3-Port": "COM1", "PRIME-PP 4 MIN ADU AFFECTED": "200", "470 Shutter-MinVolts": "0.0000", "PRIME-PP 3 ENABLED": "Yes", "470 AO-Description": "NI DAC", "PRIME-Description": "PVCAM API device adapter", "520 Shutter-MaxVolts": "5.0000", "520 Shutter-Volts": "0.0000", "528 AO-Sequenceable": "1", "PRIME-Binning": "1x1", "555 Shutter-MaxVolts": "5.0000", "528 Shutter-TriggerSequenceLength": "1000", "405 Shutter-Block voltage": "false", "XLIGHT Spin motor-State": "1", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "Vincent-D3-Time to open (ms)": "35.0000", "Vincent-D3-Time to close (ms)": "35.0000", "445 AO-TriggerSampleRate": "100000", "640 Shutter-IOChannel": "Dev1/ao14", "555 AO-Sequenceable": "1", "520 AO-Name": "AnalogIO", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "470 AO-Block voltage": "false", "COM1-Verbose": "1", "COM9-AnswerTimeout": "10000.0000", "528 Shutter-Name": "AnalogIO", "PRIME-Trigger Timeout (secs)": "10", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "NextFrame": 0, "470 AO-TriggerSequenceLength": "1000", "PRIME-TransposeXY": "0", "445 AO-TriggerSequenceLength": "1000", "COM1-Name": "COM1", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "COM9-DataBits": "8", "COM1-AnswerTimeout": "500.0000", "Camera": "", "TE2000-Version": "V2.50-V2.50-V2.10", "528 Shutter-Volts": "0.0000", "XLIGHT Hub-Name": "XLIGHT Hub", "PIEZO-Description": "Ludl stage driver adapter", "PositionName": "Pos34", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSampleRate": "100000", "PRIME-FanSpeedSetpoint": "High", "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "405 AO-TriggerSampleRate": "100000", "470 Shutter-Block voltage": "false", "470-State": "0", "COM3-Parity": "None", "405 AO-Volts": "0.0000", "PRIME-TransposeMirrorY": "0", "PRIME-ChipName": "GS144BSI", "470 AO-Volts": "0.0000", "PRIME-TransposeMirrorX": "0", "445 Shutter-SupportsTriggering": "0", "470 Shutter-SupportsTriggering": "0", "XLIGHT Hub-Description": "XLIGHT hub", "PRIME-PreampOffLimit": "10000", "470 AO-Sequenceable": "1", "528 Shutter-MaxVolts": "5.0000", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "Core-XYStage": "XYStage", "470 AO-TriggerSampleRate": "100000", "PRIME-Timing-PreTriggerDelayNs": "24672000.0000", "528-DA Device": "528 Shutter", "Focus-Description": "Nikon TE2000 focus adapter", "FilterBlock-State": "5", "520 AO-Demo": "No", "COM3-DelayBetweenCharsMs": "0.0000", "470 Shutter-Volts": "0.0000", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "PRIME-PP 2 THRESHOLD": "125", "PRIME-PP 4 THRESHOLD": "75", "XYStage-Acceleration": "4.0000", "470 Shutter-Demo": "No", "PRIME-Name": "PMPCIECam00", "640-DA Device": "640 Shutter", "405 AO-Block voltage": "false", "ElapsedTime-ms": 1399386, "PRIME-Offset": "100", "445 AO-IOChannel": "Dev1/ao1", "640 AO-MaxVolts": "5.0000", "PositionIndex": 34, "PRIME-PP 1 ENABLED": "Yes", "COM1-BaudRate": "9600", "640-State": "0", "COM3-Verbose": "1", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "LudlController-TransmissionDelay": "0", "PRIME-FullWellCapacity": "62000", "PRIME-SMARTStreamingEnabled": "No", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "640 Shutter-Description": "NI DAC", "528-Description": "DA device that is used as a shutter", "COM4-Description": "Serial port driver (boost:asio)", "405 Shutter-Description": "NI DAC", "TE2000-Name": "TE2000", "528 Shutter-Description": "NI DAC", "COM9-Description": "Serial port driver (boost:asio)", "LudlController-Name": "LudlController", "445 Shutter-TriggerSequenceLength": "1000", "PRIME-SerialNumber": "A18B203008", "445 AO-Volts": "0.0000", "PRIME-X-dimension": "1200", "PRIME-ShutterCloseDelay": "0", "XLIGHT Disk slider-State": "2", "Position": "Default", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 AO-IOChannel": "Dev1/ao5", "520 AO-IOChannel": "Dev1/ao4", "528 AO-TriggerSequenceLength": "1000", "405 Shutter-MaxVolts": "5.0000", "470-DA Device": "470 Shutter", "PRIME-FTCapable": "No", "Core-ChannelGroup": "Channels", "555 AO-SupportsTriggering": "0", "PRIME-Y-dimension": "1200", "Frame": 0, "555 Shutter-Sequenceable": "1", "PRIME-Timing-ExposureTimeNs": "499998000.0000", "Core-ImageProcessor": "", "528-State": "0", "520 AO-Block voltage": "false", "XLIGHT Excitation Wheel-Label": "QUAD", "640 Shutter-Demo": "No", "COM3-StopBits": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "520 Shutter-IOChannel": "Dev1/ao11", "Width": 1200, "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-SupportsTriggering": "0", "640 AO-TriggerSampleRate": "100000", "520-Description": "DA device that is used as a shutter", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "405 Shutter-Sequenceable": "1", "405 Shutter-SupportsTriggering": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-TriggerSampleRate": "100000", "640 Shutter-MaxVolts": "5.0000", "555 AO-MaxVolts": "5.0000", "520 AO-TriggerSequenceLength": "1000", "COM3-BaudRate": "9600", "COM9-BaudRate": "9600", "Vincent-D3-Channel #": "Ch. #1", "PRIME-PMode": "Normal", "OpticalPath-Label": "Left(100)", "BitDepth": 16, "555 AO-TriggerSequenceLength": "1000", "445 Shutter-Description": "NI DAC", "405 AO-Sequenceable": "1", "PRIME-AcquisitionMethod": "Callbacks", "555 AO-Name": "AnalogIO", "PRIME-ShutterMode": "Pre-Exposure", "COM3-Handshaking": "Off", "445 AO-SupportsTriggering": "0", "ROI": "0-0-1200-1200", "COM9-Verbose": "1", "PRIME-Color": "OFF", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "PRIME-ShutterOpenDelay": "0", "COM1-Parity": "None", "Slice": 3, "SlicePosition": -20, "COM9-DelayBetweenCharsMs": "0.0000", "555-Name": "DA Shutter", "PIEZO-LudlSingleAxisName": "B", "XLIGHT Emission Wheel-Label": "585/11(T)", "640 Shutter-Name": "AnalogIO", "640 AO-Sequenceable": "1", "520 Shutter-Demo": "No", "528 Shutter-Demo": "No", "470-Name": "DA Shutter", "520-DA Device": "520 Shutter", "XYStage-Description": "Ludl XY stage driver adapter", "445 Shutter-MinVolts": "0.0000", "528 AO-TriggerSampleRate": "100000", "405 AO-MinVolts": "0.0000", "405 Shutter-TriggerSequenceLength": "1000", "Height": 1200, "555 AO-Block voltage": "false", "640 Shutter-Volts": "0.0000", "PixelType": "GRAY16", "445 Shutter-Block voltage": "false", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "520 Shutter-TriggerSequenceLength": "1000", "COM9-Name": "COM9", "Core-SLM": "", "555 AO-Volts": "4.5000", "555 Shutter-Block voltage": "false", "XYStage-Speed": "1800.0000", "520 AO-MaxVolts": "5.0000", "528 Shutter-SupportsTriggering": "0", "COM1-Description": "Serial port driver (boost:asio)", "PRIME-MetadataEnabled": "No", "520 Shutter-MinVolts": "0.0000", "470 Shutter-Sequenceable": "1", "PRIME-PreampDelay": "0", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-MinVolts": "0.0000", "ChannelIndex": 1, "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Core-AutoShutter": "1", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "Exposure-ms": 160, "PRIME-PP 2 ENABLED": "Yes", "640 Shutter-TriggerSampleRate": "100000", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "470 Shutter-IOChannel": "Dev1/ao10", "405 AO-Demo": "No", "PixelSizeUm": 1.1, "470 AO-Demo": "No", "470 Shutter-MaxVolts": "5.0000", "Vincent-D3-Name": "Vincent-D3", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FileName": "img_000000000_Il-T_003.tif", "LudlController-Reset": "Operate", "PRIME-Exposure": "160.00", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "445-Name": "DA Shutter", "555 Shutter-Name": "AnalogIO", "520 Shutter-TriggerSampleRate": "100000", "445 Shutter-IOChannel": "Dev1/ao9", "PRIME-TransposeCorrection": "0", "XYStage-StepSize": "0.1000", "445-Description": "DA device that is used as a shutter", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 AO-IOChannel": "Dev1/ao7", "520 Shutter-Name": "AnalogIO", "COM3-DataBits": "8", "640 AO-Volts": "0.0000", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "555 Shutter-IOChannel": "Dev1/ao13", "WaitInterval": null, "520 Shutter-Sequenceable": "1", "YPositionUm": 545.6, "445-State": "0", "445-DA Device": "445 Shutter", "PRIME-CircularBufferAutoSize": "ON", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "555 AO-TriggerSampleRate": "100000", "445 Shutter-Volts": "0.0000", "XLIGHT Hub-Port": "COM3", "Core-AutoFocus": "", "COM4-Handshaking": "Off", "528 Shutter-MinVolts": "0.0000", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "445 Shutter-Name": "AnalogIO", "528 Shutter-Sequenceable": "1", "XYStage-TransposeMirrorX": "0", "XYStage-TransposeMirrorY": "0", "PRIME-ReadoutRate": "100MHz 16bit", "XYStage-Name": "Stage", "640 AO-SupportsTriggering": "0", "640-Name": "DA Shutter", "640 Shutter-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "640 AO-Block voltage": "false", "445 Shutter-Sequenceable": "1", "470 AO-MaxVolts": "5.0000", "COM4-BaudRate": "9600", "470-Description": "DA device that is used as a shutter", "520 Shutter-Description": "NI DAC", "PRIME-CCDTemperature": "-14.8500", "Time": "2019-12-19 09:59:29 -0500", "640 AO-TriggerSequenceLength": "1000", "470 AO-Name": "AnalogIO", "XLIGHT Disk slider-Label": "Disk Pos 40um", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "Binning": "1x1", "TE2000-ModelType": "1", "COM3-AnswerTimeout": "500.0000", "555 Shutter-SupportsTriggering": "0", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "TE2000-Port": "COM4", "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Description": "NI DAC", "COM4-AnswerTimeout": "500.0000", "COM1-DelayBetweenCharsMs": "0.0000", "FilterBlock-Label": "Confocal", "445 AO-Name": "AnalogIO", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-Timing-ReadoutTimeNs": "24672000.0000", "528 AO-Demo": "No", "520 AO-Sequenceable": "1", "555-Description": "DA device that is used as a shutter", "405-DA Device": "405 Shutter", "405 AO-TriggerSequenceLength": "1000", "640 AO-MinVolts": "0.0000", "640 Shutter-Block voltage": "false", "FrameIndex": 0, "PRIME-Port": "CMOS", "555 AO-MinVolts": "0.0000", "COM3-Description": "Serial port driver (boost:asio)", "528 AO-Description": "NI DAC", "555 AO-IOChannel": "Dev1/ao6", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "555 AO-TriggerInputLine": "/Dev1/PFI0", "PRIME-ActualInterval-ms": "390.5200", "LudlController-Port": "COM9", "445 Shutter-Demo": "No", "PRIME-Trigger-Expose Out-Mux": "1", "SliceIndex": 3, "PRIME-CCDTemperatureSetPoint": "-15.0000", "405 Shutter-MinVolts": "0.0000", "PRIME-ExposeOutMode": "First Row", "AxisPositions": null, "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Volts": "5.0000", "528-Name": "DA Shutter", "555-State": "1", "XLIGHT Dichroic-Label": "QUAD", "445 AO-Sequenceable": "1", "405-Name": "DA Shutter", "405 AO-IOChannel": "Dev1/ao0", "Core-Camera": "PRIME", "Focus-Name": "Focus", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "405 Shutter-IOChannel": "Dev1/ao8", "640 AO-Demo": "No", "640 Shutter-SupportsTriggering": "0", "445 AO-Demo": "No", "555-DA Device": "555 Shutter", "470 Shutter-Description": "NI DAC", "528 AO-Volts": "0.0000", "COM1-DataBits": "8", "Vincent-D3-Address": "x", "520 AO-SupportsTriggering": "0", "405-Description": "DA device that is used as a shutter", "640 Shutter-MinVolts": "0.0000", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "405 AO-Name": "AnalogIO", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "640 AO-TriggerInputLine": "/Dev1/PFI0", "405 AO-TriggerInputLine": "/Dev1/PFI0", "640-Description": "DA device that is used as a shutter", "445 Shutter-TriggerSampleRate": "100000", "470 Shutter-TriggerSequenceLength": "1000", "COM1-StopBits": "1", "520 Shutter-SupportsTriggering": "0", "405-State": "0", "Core-Initialize": "1", "XLIGHT Emission Wheel-State": "3", "520 AO-Description": "NI DAC", "528 AO-TriggerInputLine": "/Dev1/PFI0", "445 Shutter-MaxVolts": "5.0000", "COM9-Handshaking": "Off", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "XLIGHT Spin motor-Label": "On (spinning)", "PRIME-PP 5 ENABLED": "No", "UUID": "c52f29fa-4e68-4528-9490-76dece63ce56", "PRIME-TriggerMode": "Internal Trigger", "COM1-Handshaking": "Off", "PRIME-Timing-PostTriggerDelayNs": "49344000.0000", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "Focus-Position": "17741.5000", "528 Shutter-Block voltage": "false", "Channel": "Il-T", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "555 Shutter-Demo": "No", "405 Shutter-Demo": "No", "528 AO-Name": "AnalogIO", "520-State": "0", "Core-Galvo": "", "405 Shutter-TriggerSampleRate": "100000", "Focus-FocusStepSize_(nm)": "100", "PRIME-PixelType": "16bit", "COM4-DataBits": "8", "470 AO-IOChannel": "Dev1/ao2", "520 AO-MinVolts": "0.0000", "COM9-Parity": "None", "PRIME-CircularBufferEnabled": "ON", "PRIME-PP 6 Reset": "No", "640 AO-Description": "NI DAC", "Source": "PRIME", "445 AO-MinVolts": "0.0000", "PIEZO-Name": "Stage", "445 AO-Description": "NI DAC", "PRIME-CircularBufferFrameCount": "46", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "520 AO-Volts": "0.0000", "528 Shutter-TriggerSampleRate": "100000", "XPositionUm": -1324.9, "OpticalPath-State": "4", "PRIME-PP 3 THRESHOLD": "80", "PRIME-PVCAM Version": "3.7.1", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Gain": "1-HDR", "640 Shutter-TriggerSequenceLength": "1000", "528 AO-SupportsTriggering": "0", "445 AO-TriggerInputLine": "/Dev1/PFI0", "OpticalPath-Name": "OpticalPath", "PRIME-PP 4 ENABLED": "Yes", "PRIME-PVCAM Adapter Version": "1.3.29", "COM3-Name": "COM3", "405 AO-MaxVolts": "5.0000", "520 Shutter-Block voltage": "false", "PRIME-Timing-ClearingTimeNs": "49344000.0000", "Core-Focus": "PIEZO", "528 AO-Block voltage": "false", "TE2000-Description": "Nikon TE2000 hub", "528 AO-MinVolts": "0.0000", "COM4-Parity": "None", "Summary": { "Slices": 11, "ChContrastMin": [ 0, 0, 0, 0, 0, 0 ], "PixelAspect": 1, "SlicesFirst": true, "Interval_ms": 0, "Time": "2019-12-19 09:59:21 -0500", "Prefix": "seq_13", "InitialPositionList": [ { "Label": "Pos0", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.21], "Focus": [17741.5], "XYStage": [ 44542.200000000004, 31636.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos1", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.22], "Focus": [17741.4], "XYStage": [ 43801.100000000006, 31637.9 ] }, "GridColumnIndex": 0 }, { "Label": "Pos2", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.5], "XYStage": [ 38561.3, 32040.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos3", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.185], "Focus": [17741.3], "XYStage": [ 37698.4, 32041.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos4", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.16], "Focus": [17741.5], "XYStage": [ 32375.4, 31758.9 ] }, "GridColumnIndex": 0 }, { "Label": "Pos5", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.17000000000002], "Focus": [17741.3], "XYStage": [ 31770.7, 31758.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos6", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.4], "XYStage": [ 26536.800000000003, 31068.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos7", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.205], "Focus": [17741.4], "XYStage": [ 25420.2, 31068.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos8", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.85], "Focus": [17741.3], "XYStage": [ 21059, 31508 ] }, "GridColumnIndex": 0 }, { "Label": "Pos9", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.82], "Focus": [17741.3], "XYStage": [ 19731.9, 31506.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos10", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.79], "Focus": [17741.4], "XYStage": [ 14528.5, 32007.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos11", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.83], "Focus": [17741.2], "XYStage": [ 13830.300000000001, 32009.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos12", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.79], "Focus": [17741.3], "XYStage": [ 8775.300000000001, 32017.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos13", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.815], "Focus": [17741.3], "XYStage": [ 8109.200000000001, 32008.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos14", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.805], "Focus": [17741.3], "XYStage": [ 3483.3, 31908.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos15", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.82], "Focus": [17741.2], "XYStage": [ 2686.8, 31903.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos16", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [211.48000000000002], "Focus": [17741.1], "XYStage": [ -1978.9, 31973.600000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos17", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [211.51500000000001], "Focus": [17741.1], "XYStage": [ -2957.8, 31982.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos18", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.195], "Focus": [17741.5], "XYStage": [ 47747.4, -576.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos19", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.23000000000002], "Focus": [17741.6], "XYStage": [ 47053.9, -576.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos20", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.205], "Focus": [17741.4], "XYStage": [ 41871.200000000004, -479.8 ] }, "GridColumnIndex": 0 }, { "Label": "Pos21", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.4], "XYStage": [ 40882.600000000006, -392 ] }, "GridColumnIndex": 0 }, { "Label": "Pos22", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.24], "Focus": [17741.4], "XYStage": [ 35905.6, -135.3 ] }, "GridColumnIndex": 0 }, { "Label": "Pos23", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.195], "Focus": [17741.5], "XYStage": [ 34990.9, -132.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos24", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.46], "Focus": [17741.4], "XYStage": [ 29678.100000000002, -958.1 ] }, "GridColumnIndex": 0 }, { "Label": "Pos25", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.44], "Focus": [17741.5], "XYStage": [ 28983.600000000002, -939.4000000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos26", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.475], "Focus": [17741.5], "XYStage": [ 23019.7, -122 ] }, "GridColumnIndex": 0 }, { "Label": "Pos27", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.44], "Focus": [17741.7], "XYStage": [ 22428, -116.80000000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos28", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.46], "Focus": [17741.7], "XYStage": [ 16364.900000000001, -181.10000000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos29", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.48000000000002], "Focus": [17741.5], "XYStage": [ 15712.1, -180 ] }, "GridColumnIndex": 0 }, { "Label": "Pos30", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.45000000000002], "Focus": [17741.7], "XYStage": [ 10346.1, 77.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos31", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.49], "Focus": [17741.4], "XYStage": [ 9755.5, 85.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos32", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.475], "Focus": [17741.5], "XYStage": [ 4775.5, 293.90000000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos33", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.445], "Focus": [17741.4], "XYStage": [ 3954.9, 293.90000000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos34", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.455], "Focus": [17741.4], "XYStage": [ -1324.9, 545.6 ] }, "GridColumnIndex": 0 }, { "Label": "Pos35", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.445], "Focus": [17741.3], "XYStage": [ -2097, 576.6 ] }, "GridColumnIndex": 0 } ], "z-step_um": 10, "GridRow": 0, "BitDepth": 16, "Depth": 2, "Channels": 6, "Source": "Micro-Manager", "ChColors": [ -16711681, -256, -65281, -1, -1, -16776961 ], "MetadataVersion": 10, "KeepShutterOpenChannels": true, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536, 65536 ], "CustomIntervals_ms": [], "Height": 1200, "MicroManagerVersion": "1.4.23 20180219", "GridColumn": 0, "UUID": "18808e7c-f2e6-47b3-90d8-1d779ce87dbd", "PixelType": "GRAY16", "Width": 1200, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\Yan\\20191218-YC113-S7S8-miseq", "Frames": 1, "Comment": "", "UserName": "Nikon", "PixelSize_um": 1.1, "ROI": [ 0, 0, 1200, 1200 ], "Date": "2019-12-19", "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC", "P-DAPI-high" ], "KeepShutterOpenSlices": true, "IJType": 1, "ComputerName": "ZadorConfocal2", "Positions": 36, "PositionIndex": 34, "CameraTimeout": 20000, "TimeFirst": false }, "640 AO-Name": "AnalogIO", "Vincent-D3-Command": "Close" }, "FrameKey-0-1-4": { "COM4-StopBits": "1", "528 AO-MaxVolts": "5.0000", "XLIGHT Excitation Wheel-State": "1", "470 Shutter-Name": "AnalogIO", "Core-Shutter": "555", "555 AO-Demo": "No", "XLIGHT Dichroic-State": "2", "XYStage-StepSize-Y": "0.1000", "ZPositionUm": 208.455, "XYStage-StepSize-X": "0.1000", "PRIME-FirmwareVersion": "20.28", "PRIME-ClearCycles": "2", "520-Name": "DA Shutter", "405 Shutter-Name": "AnalogIO", "PIEZO-StepSize": "0.0050", "PRIME-ClearMode": "Pre-Exposure", "470 AO-MinVolts": "0.0000", "405 AO-SupportsTriggering": "1", "CameraChannelIndex": 0, "XYStage-StartSpeed": "500.0000", "COM9-StopBits": "2", "405 AO-Description": "NI DAC", "COM4-DelayBetweenCharsMs": "0.0000", "445 AO-Block voltage": "false", "520 AO-TriggerInputLine": "/Dev1/PFI0", "555 AO-Description": "NI DAC", "520 AO-TriggerSampleRate": "100000", "COM4-Verbose": "1", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM4-Name": "COM4", "405 Shutter-Volts": "0.0000", "Vincent-D3-Port": "COM1", "PRIME-PP 4 MIN ADU AFFECTED": "200", "470 Shutter-MinVolts": "0.0000", "PRIME-PP 3 ENABLED": "Yes", "470 AO-Description": "NI DAC", "PRIME-Description": "PVCAM API device adapter", "520 Shutter-MaxVolts": "5.0000", "520 Shutter-Volts": "0.0000", "528 AO-Sequenceable": "1", "PRIME-Binning": "1x1", "555 Shutter-MaxVolts": "5.0000", "528 Shutter-TriggerSequenceLength": "1000", "405 Shutter-Block voltage": "false", "XLIGHT Spin motor-State": "1", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "Vincent-D3-Time to open (ms)": "35.0000", "Vincent-D3-Time to close (ms)": "35.0000", "445 AO-TriggerSampleRate": "100000", "640 Shutter-IOChannel": "Dev1/ao14", "555 AO-Sequenceable": "1", "520 AO-Name": "AnalogIO", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "470 AO-Block voltage": "false", "COM1-Verbose": "1", "COM9-AnswerTimeout": "10000.0000", "528 Shutter-Name": "AnalogIO", "PRIME-Trigger Timeout (secs)": "10", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "NextFrame": 0, "470 AO-TriggerSequenceLength": "1000", "PRIME-TransposeXY": "0", "445 AO-TriggerSequenceLength": "1000", "COM1-Name": "COM1", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "COM9-DataBits": "8", "COM1-AnswerTimeout": "500.0000", "Camera": "", "TE2000-Version": "V2.50-V2.50-V2.10", "528 Shutter-Volts": "0.0000", "XLIGHT Hub-Name": "XLIGHT Hub", "PIEZO-Description": "Ludl stage driver adapter", "PositionName": "Pos34", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSampleRate": "100000", "PRIME-FanSpeedSetpoint": "High", "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "405 AO-TriggerSampleRate": "100000", "470 Shutter-Block voltage": "false", "470-State": "0", "COM3-Parity": "None", "405 AO-Volts": "0.0000", "PRIME-TransposeMirrorY": "0", "PRIME-ChipName": "GS144BSI", "470 AO-Volts": "0.0000", "PRIME-TransposeMirrorX": "0", "445 Shutter-SupportsTriggering": "0", "470 Shutter-SupportsTriggering": "0", "XLIGHT Hub-Description": "XLIGHT hub", "PRIME-PreampOffLimit": "10000", "470 AO-Sequenceable": "1", "528 Shutter-MaxVolts": "5.0000", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "Core-XYStage": "XYStage", "470 AO-TriggerSampleRate": "100000", "PRIME-Timing-PreTriggerDelayNs": "24672000.0000", "528-DA Device": "528 Shutter", "Focus-Description": "Nikon TE2000 focus adapter", "FilterBlock-State": "5", "520 AO-Demo": "No", "COM3-DelayBetweenCharsMs": "0.0000", "470 Shutter-Volts": "0.0000", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "PRIME-PP 2 THRESHOLD": "125", "PRIME-PP 4 THRESHOLD": "75", "XYStage-Acceleration": "4.0000", "470 Shutter-Demo": "No", "PRIME-Name": "PMPCIECam00", "640-DA Device": "640 Shutter", "405 AO-Block voltage": "false", "ElapsedTime-ms": 1399781, "PRIME-Offset": "100", "445 AO-IOChannel": "Dev1/ao1", "640 AO-MaxVolts": "5.0000", "PositionIndex": 34, "PRIME-PP 1 ENABLED": "Yes", "COM1-BaudRate": "9600", "640-State": "0", "COM3-Verbose": "1", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "LudlController-TransmissionDelay": "0", "PRIME-FullWellCapacity": "62000", "PRIME-SMARTStreamingEnabled": "No", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "640 Shutter-Description": "NI DAC", "528-Description": "DA device that is used as a shutter", "COM4-Description": "Serial port driver (boost:asio)", "405 Shutter-Description": "NI DAC", "TE2000-Name": "TE2000", "528 Shutter-Description": "NI DAC", "COM9-Description": "Serial port driver (boost:asio)", "LudlController-Name": "LudlController", "445 Shutter-TriggerSequenceLength": "1000", "PRIME-SerialNumber": "A18B203008", "445 AO-Volts": "0.0000", "PRIME-X-dimension": "1200", "PRIME-ShutterCloseDelay": "0", "XLIGHT Disk slider-State": "2", "Position": "Default", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 AO-IOChannel": "Dev1/ao5", "520 AO-IOChannel": "Dev1/ao4", "528 AO-TriggerSequenceLength": "1000", "405 Shutter-MaxVolts": "5.0000", "470-DA Device": "470 Shutter", "PRIME-FTCapable": "No", "Core-ChannelGroup": "Channels", "555 AO-SupportsTriggering": "0", "PRIME-Y-dimension": "1200", "Frame": 0, "555 Shutter-Sequenceable": "1", "PRIME-Timing-ExposureTimeNs": "499998000.0000", "Core-ImageProcessor": "", "528-State": "0", "520 AO-Block voltage": "false", "XLIGHT Excitation Wheel-Label": "QUAD", "640 Shutter-Demo": "No", "COM3-StopBits": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "520 Shutter-IOChannel": "Dev1/ao11", "Width": 1200, "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-SupportsTriggering": "0", "640 AO-TriggerSampleRate": "100000", "520-Description": "DA device that is used as a shutter", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "405 Shutter-Sequenceable": "1", "405 Shutter-SupportsTriggering": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-TriggerSampleRate": "100000", "640 Shutter-MaxVolts": "5.0000", "555 AO-MaxVolts": "5.0000", "520 AO-TriggerSequenceLength": "1000", "COM3-BaudRate": "9600", "COM9-BaudRate": "9600", "Vincent-D3-Channel #": "Ch. #1", "PRIME-PMode": "Normal", "OpticalPath-Label": "Left(100)", "BitDepth": 16, "555 AO-TriggerSequenceLength": "1000", "445 Shutter-Description": "NI DAC", "405 AO-Sequenceable": "1", "PRIME-AcquisitionMethod": "Callbacks", "555 AO-Name": "AnalogIO", "PRIME-ShutterMode": "Pre-Exposure", "COM3-Handshaking": "Off", "445 AO-SupportsTriggering": "0", "ROI": "0-0-1200-1200", "COM9-Verbose": "1", "PRIME-Color": "OFF", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "PRIME-ShutterOpenDelay": "0", "COM1-Parity": "None", "Slice": 4, "SlicePosition": -10, "COM9-DelayBetweenCharsMs": "0.0000", "555-Name": "DA Shutter", "PIEZO-LudlSingleAxisName": "B", "XLIGHT Emission Wheel-Label": "585/11(T)", "640 Shutter-Name": "AnalogIO", "640 AO-Sequenceable": "1", "520 Shutter-Demo": "No", "528 Shutter-Demo": "No", "470-Name": "DA Shutter", "520-DA Device": "520 Shutter", "XYStage-Description": "Ludl XY stage driver adapter", "445 Shutter-MinVolts": "0.0000", "528 AO-TriggerSampleRate": "100000", "405 AO-MinVolts": "0.0000", "405 Shutter-TriggerSequenceLength": "1000", "Height": 1200, "555 AO-Block voltage": "false", "640 Shutter-Volts": "0.0000", "PixelType": "GRAY16", "445 Shutter-Block voltage": "false", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "520 Shutter-TriggerSequenceLength": "1000", "COM9-Name": "COM9", "Core-SLM": "", "555 AO-Volts": "4.5000", "555 Shutter-Block voltage": "false", "XYStage-Speed": "1800.0000", "520 AO-MaxVolts": "5.0000", "528 Shutter-SupportsTriggering": "0", "COM1-Description": "Serial port driver (boost:asio)", "PRIME-MetadataEnabled": "No", "520 Shutter-MinVolts": "0.0000", "470 Shutter-Sequenceable": "1", "PRIME-PreampDelay": "0", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-MinVolts": "0.0000", "ChannelIndex": 1, "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Core-AutoShutter": "1", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "Exposure-ms": 160, "PRIME-PP 2 ENABLED": "Yes", "640 Shutter-TriggerSampleRate": "100000", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "470 Shutter-IOChannel": "Dev1/ao10", "405 AO-Demo": "No", "PixelSizeUm": 1.1, "470 AO-Demo": "No", "470 Shutter-MaxVolts": "5.0000", "Vincent-D3-Name": "Vincent-D3", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FileName": "img_000000000_Il-T_004.tif", "LudlController-Reset": "Operate", "PRIME-Exposure": "160.00", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "445-Name": "DA Shutter", "555 Shutter-Name": "AnalogIO", "520 Shutter-TriggerSampleRate": "100000", "445 Shutter-IOChannel": "Dev1/ao9", "PRIME-TransposeCorrection": "0", "XYStage-StepSize": "0.1000", "445-Description": "DA device that is used as a shutter", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 AO-IOChannel": "Dev1/ao7", "520 Shutter-Name": "AnalogIO", "COM3-DataBits": "8", "640 AO-Volts": "0.0000", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "555 Shutter-IOChannel": "Dev1/ao13", "WaitInterval": null, "520 Shutter-Sequenceable": "1", "YPositionUm": 545.6, "445-State": "0", "445-DA Device": "445 Shutter", "PRIME-CircularBufferAutoSize": "ON", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "555 AO-TriggerSampleRate": "100000", "445 Shutter-Volts": "0.0000", "XLIGHT Hub-Port": "COM3", "Core-AutoFocus": "", "COM4-Handshaking": "Off", "528 Shutter-MinVolts": "0.0000", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "445 Shutter-Name": "AnalogIO", "528 Shutter-Sequenceable": "1", "XYStage-TransposeMirrorX": "0", "XYStage-TransposeMirrorY": "0", "PRIME-ReadoutRate": "100MHz 16bit", "XYStage-Name": "Stage", "640 AO-SupportsTriggering": "0", "640-Name": "DA Shutter", "640 Shutter-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "640 AO-Block voltage": "false", "445 Shutter-Sequenceable": "1", "470 AO-MaxVolts": "5.0000", "COM4-BaudRate": "9600", "470-Description": "DA device that is used as a shutter", "520 Shutter-Description": "NI DAC", "PRIME-CCDTemperature": "-14.8500", "Time": "2019-12-19 09:59:30 -0500", "640 AO-TriggerSequenceLength": "1000", "470 AO-Name": "AnalogIO", "XLIGHT Disk slider-Label": "Disk Pos 40um", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "Binning": "1x1", "TE2000-ModelType": "1", "COM3-AnswerTimeout": "500.0000", "555 Shutter-SupportsTriggering": "0", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "TE2000-Port": "COM4", "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Description": "NI DAC", "COM4-AnswerTimeout": "500.0000", "COM1-DelayBetweenCharsMs": "0.0000", "FilterBlock-Label": "Confocal", "445 AO-Name": "AnalogIO", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-Timing-ReadoutTimeNs": "24672000.0000", "528 AO-Demo": "No", "520 AO-Sequenceable": "1", "555-Description": "DA device that is used as a shutter", "405-DA Device": "405 Shutter", "405 AO-TriggerSequenceLength": "1000", "640 AO-MinVolts": "0.0000", "640 Shutter-Block voltage": "false", "FrameIndex": 0, "PRIME-Port": "CMOS", "555 AO-MinVolts": "0.0000", "COM3-Description": "Serial port driver (boost:asio)", "528 AO-Description": "NI DAC", "555 AO-IOChannel": "Dev1/ao6", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "555 AO-TriggerInputLine": "/Dev1/PFI0", "PRIME-ActualInterval-ms": "390.5200", "LudlController-Port": "COM9", "445 Shutter-Demo": "No", "PRIME-Trigger-Expose Out-Mux": "1", "SliceIndex": 4, "PRIME-CCDTemperatureSetPoint": "-15.0000", "405 Shutter-MinVolts": "0.0000", "PRIME-ExposeOutMode": "First Row", "AxisPositions": null, "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Volts": "5.0000", "528-Name": "DA Shutter", "555-State": "1", "XLIGHT Dichroic-Label": "QUAD", "445 AO-Sequenceable": "1", "405-Name": "DA Shutter", "405 AO-IOChannel": "Dev1/ao0", "Core-Camera": "PRIME", "Focus-Name": "Focus", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "405 Shutter-IOChannel": "Dev1/ao8", "640 AO-Demo": "No", "640 Shutter-SupportsTriggering": "0", "445 AO-Demo": "No", "555-DA Device": "555 Shutter", "470 Shutter-Description": "NI DAC", "528 AO-Volts": "0.0000", "COM1-DataBits": "8", "Vincent-D3-Address": "x", "520 AO-SupportsTriggering": "0", "405-Description": "DA device that is used as a shutter", "640 Shutter-MinVolts": "0.0000", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "405 AO-Name": "AnalogIO", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "640 AO-TriggerInputLine": "/Dev1/PFI0", "405 AO-TriggerInputLine": "/Dev1/PFI0", "640-Description": "DA device that is used as a shutter", "445 Shutter-TriggerSampleRate": "100000", "470 Shutter-TriggerSequenceLength": "1000", "COM1-StopBits": "1", "520 Shutter-SupportsTriggering": "0", "405-State": "0", "Core-Initialize": "1", "XLIGHT Emission Wheel-State": "3", "520 AO-Description": "NI DAC", "528 AO-TriggerInputLine": "/Dev1/PFI0", "445 Shutter-MaxVolts": "5.0000", "COM9-Handshaking": "Off", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "XLIGHT Spin motor-Label": "On (spinning)", "PRIME-PP 5 ENABLED": "No", "UUID": "f27ccf17-4662-4ece-915a-6ff4afbbdf9a", "PRIME-TriggerMode": "Internal Trigger", "COM1-Handshaking": "Off", "PRIME-Timing-PostTriggerDelayNs": "49344000.0000", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "Focus-Position": "17741.5000", "528 Shutter-Block voltage": "false", "Channel": "Il-T", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "555 Shutter-Demo": "No", "405 Shutter-Demo": "No", "528 AO-Name": "AnalogIO", "520-State": "0", "Core-Galvo": "", "405 Shutter-TriggerSampleRate": "100000", "Focus-FocusStepSize_(nm)": "100", "PRIME-PixelType": "16bit", "COM4-DataBits": "8", "470 AO-IOChannel": "Dev1/ao2", "520 AO-MinVolts": "0.0000", "COM9-Parity": "None", "PRIME-CircularBufferEnabled": "ON", "PRIME-PP 6 Reset": "No", "640 AO-Description": "NI DAC", "Source": "PRIME", "445 AO-MinVolts": "0.0000", "PIEZO-Name": "Stage", "445 AO-Description": "NI DAC", "PRIME-CircularBufferFrameCount": "46", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "520 AO-Volts": "0.0000", "528 Shutter-TriggerSampleRate": "100000", "XPositionUm": -1324.9, "OpticalPath-State": "4", "PRIME-PP 3 THRESHOLD": "80", "PRIME-PVCAM Version": "3.7.1", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Gain": "1-HDR", "640 Shutter-TriggerSequenceLength": "1000", "528 AO-SupportsTriggering": "0", "445 AO-TriggerInputLine": "/Dev1/PFI0", "OpticalPath-Name": "OpticalPath", "PRIME-PP 4 ENABLED": "Yes", "PRIME-PVCAM Adapter Version": "1.3.29", "COM3-Name": "COM3", "405 AO-MaxVolts": "5.0000", "520 Shutter-Block voltage": "false", "PRIME-Timing-ClearingTimeNs": "49344000.0000", "Core-Focus": "PIEZO", "528 AO-Block voltage": "false", "TE2000-Description": "Nikon TE2000 hub", "528 AO-MinVolts": "0.0000", "COM4-Parity": "None", "Summary": { "Slices": 11, "ChContrastMin": [ 0, 0, 0, 0, 0, 0 ], "PixelAspect": 1, "SlicesFirst": true, "Interval_ms": 0, "Time": "2019-12-19 09:59:21 -0500", "Prefix": "seq_13", "InitialPositionList": [ { "Label": "Pos0", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.21], "Focus": [17741.5], "XYStage": [ 44542.200000000004, 31636.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos1", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.22], "Focus": [17741.4], "XYStage": [ 43801.100000000006, 31637.9 ] }, "GridColumnIndex": 0 }, { "Label": "Pos2", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.5], "XYStage": [ 38561.3, 32040.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos3", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.185], "Focus": [17741.3], "XYStage": [ 37698.4, 32041.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos4", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.16], "Focus": [17741.5], "XYStage": [ 32375.4, 31758.9 ] }, "GridColumnIndex": 0 }, { "Label": "Pos5", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.17000000000002], "Focus": [17741.3], "XYStage": [ 31770.7, 31758.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos6", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.4], "XYStage": [ 26536.800000000003, 31068.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos7", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.205], "Focus": [17741.4], "XYStage": [ 25420.2, 31068.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos8", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.85], "Focus": [17741.3], "XYStage": [ 21059, 31508 ] }, "GridColumnIndex": 0 }, { "Label": "Pos9", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.82], "Focus": [17741.3], "XYStage": [ 19731.9, 31506.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos10", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.79], "Focus": [17741.4], "XYStage": [ 14528.5, 32007.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos11", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.83], "Focus": [17741.2], "XYStage": [ 13830.300000000001, 32009.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos12", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.79], "Focus": [17741.3], "XYStage": [ 8775.300000000001, 32017.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos13", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.815], "Focus": [17741.3], "XYStage": [ 8109.200000000001, 32008.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos14", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.805], "Focus": [17741.3], "XYStage": [ 3483.3, 31908.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos15", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.82], "Focus": [17741.2], "XYStage": [ 2686.8, 31903.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos16", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [211.48000000000002], "Focus": [17741.1], "XYStage": [ -1978.9, 31973.600000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos17", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [211.51500000000001], "Focus": [17741.1], "XYStage": [ -2957.8, 31982.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos18", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.195], "Focus": [17741.5], "XYStage": [ 47747.4, -576.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos19", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.23000000000002], "Focus": [17741.6], "XYStage": [ 47053.9, -576.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos20", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.205], "Focus": [17741.4], "XYStage": [ 41871.200000000004, -479.8 ] }, "GridColumnIndex": 0 }, { "Label": "Pos21", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.4], "XYStage": [ 40882.600000000006, -392 ] }, "GridColumnIndex": 0 }, { "Label": "Pos22", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.24], "Focus": [17741.4], "XYStage": [ 35905.6, -135.3 ] }, "GridColumnIndex": 0 }, { "Label": "Pos23", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.195], "Focus": [17741.5], "XYStage": [ 34990.9, -132.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos24", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.46], "Focus": [17741.4], "XYStage": [ 29678.100000000002, -958.1 ] }, "GridColumnIndex": 0 }, { "Label": "Pos25", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.44], "Focus": [17741.5], "XYStage": [ 28983.600000000002, -939.4000000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos26", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.475], "Focus": [17741.5], "XYStage": [ 23019.7, -122 ] }, "GridColumnIndex": 0 }, { "Label": "Pos27", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.44], "Focus": [17741.7], "XYStage": [ 22428, -116.80000000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos28", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.46], "Focus": [17741.7], "XYStage": [ 16364.900000000001, -181.10000000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos29", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.48000000000002], "Focus": [17741.5], "XYStage": [ 15712.1, -180 ] }, "GridColumnIndex": 0 }, { "Label": "Pos30", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.45000000000002], "Focus": [17741.7], "XYStage": [ 10346.1, 77.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos31", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.49], "Focus": [17741.4], "XYStage": [ 9755.5, 85.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos32", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.475], "Focus": [17741.5], "XYStage": [ 4775.5, 293.90000000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos33", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.445], "Focus": [17741.4], "XYStage": [ 3954.9, 293.90000000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos34", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.455], "Focus": [17741.4], "XYStage": [ -1324.9, 545.6 ] }, "GridColumnIndex": 0 }, { "Label": "Pos35", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.445], "Focus": [17741.3], "XYStage": [ -2097, 576.6 ] }, "GridColumnIndex": 0 } ], "z-step_um": 10, "GridRow": 0, "BitDepth": 16, "Depth": 2, "Channels": 6, "Source": "Micro-Manager", "ChColors": [ -16711681, -256, -65281, -1, -1, -16776961 ], "MetadataVersion": 10, "KeepShutterOpenChannels": true, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536, 65536 ], "CustomIntervals_ms": [], "Height": 1200, "MicroManagerVersion": "1.4.23 20180219", "GridColumn": 0, "UUID": "18808e7c-f2e6-47b3-90d8-1d779ce87dbd", "PixelType": "GRAY16", "Width": 1200, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\Yan\\20191218-YC113-S7S8-miseq", "Frames": 1, "Comment": "", "UserName": "Nikon", "PixelSize_um": 1.1, "ROI": [ 0, 0, 1200, 1200 ], "Date": "2019-12-19", "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC", "P-DAPI-high" ], "KeepShutterOpenSlices": true, "IJType": 1, "ComputerName": "ZadorConfocal2", "Positions": 36, "PositionIndex": 34, "CameraTimeout": 20000, "TimeFirst": false }, "640 AO-Name": "AnalogIO", "Vincent-D3-Command": "Close" }, "FrameKey-0-1-5": { "COM4-StopBits": "1", "528 AO-MaxVolts": "5.0000", "XLIGHT Excitation Wheel-State": "1", "470 Shutter-Name": "AnalogIO", "Core-Shutter": "555", "555 AO-Demo": "No", "XLIGHT Dichroic-State": "2", "XYStage-StepSize-Y": "0.1000", "ZPositionUm": 218.455, "XYStage-StepSize-X": "0.1000", "PRIME-FirmwareVersion": "20.28", "PRIME-ClearCycles": "2", "520-Name": "DA Shutter", "405 Shutter-Name": "AnalogIO", "PIEZO-StepSize": "0.0050", "PRIME-ClearMode": "Pre-Exposure", "470 AO-MinVolts": "0.0000", "405 AO-SupportsTriggering": "1", "CameraChannelIndex": 0, "XYStage-StartSpeed": "500.0000", "COM9-StopBits": "2", "405 AO-Description": "NI DAC", "COM4-DelayBetweenCharsMs": "0.0000", "445 AO-Block voltage": "false", "520 AO-TriggerInputLine": "/Dev1/PFI0", "555 AO-Description": "NI DAC", "520 AO-TriggerSampleRate": "100000", "COM4-Verbose": "1", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM4-Name": "COM4", "405 Shutter-Volts": "0.0000", "Vincent-D3-Port": "COM1", "PRIME-PP 4 MIN ADU AFFECTED": "200", "470 Shutter-MinVolts": "0.0000", "PRIME-PP 3 ENABLED": "Yes", "470 AO-Description": "NI DAC", "PRIME-Description": "PVCAM API device adapter", "520 Shutter-MaxVolts": "5.0000", "520 Shutter-Volts": "0.0000", "528 AO-Sequenceable": "1", "PRIME-Binning": "1x1", "555 Shutter-MaxVolts": "5.0000", "528 Shutter-TriggerSequenceLength": "1000", "405 Shutter-Block voltage": "false", "XLIGHT Spin motor-State": "1", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "Vincent-D3-Time to open (ms)": "35.0000", "Vincent-D3-Time to close (ms)": "35.0000", "445 AO-TriggerSampleRate": "100000", "640 Shutter-IOChannel": "Dev1/ao14", "555 AO-Sequenceable": "1", "520 AO-Name": "AnalogIO", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "470 AO-Block voltage": "false", "COM1-Verbose": "1", "COM9-AnswerTimeout": "10000.0000", "528 Shutter-Name": "AnalogIO", "PRIME-Trigger Timeout (secs)": "10", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "NextFrame": 0, "470 AO-TriggerSequenceLength": "1000", "PRIME-TransposeXY": "0", "445 AO-TriggerSequenceLength": "1000", "COM1-Name": "COM1", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "COM9-DataBits": "8", "COM1-AnswerTimeout": "500.0000", "Camera": "", "TE2000-Version": "V2.50-V2.50-V2.10", "528 Shutter-Volts": "0.0000", "XLIGHT Hub-Name": "XLIGHT Hub", "PIEZO-Description": "Ludl stage driver adapter", "PositionName": "Pos34", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSampleRate": "100000", "PRIME-FanSpeedSetpoint": "High", "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "405 AO-TriggerSampleRate": "100000", "470 Shutter-Block voltage": "false", "470-State": "0", "COM3-Parity": "None", "405 AO-Volts": "0.0000", "PRIME-TransposeMirrorY": "0", "PRIME-ChipName": "GS144BSI", "470 AO-Volts": "0.0000", "PRIME-TransposeMirrorX": "0", "445 Shutter-SupportsTriggering": "0", "470 Shutter-SupportsTriggering": "0", "XLIGHT Hub-Description": "XLIGHT hub", "PRIME-PreampOffLimit": "10000", "470 AO-Sequenceable": "1", "528 Shutter-MaxVolts": "5.0000", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "Core-XYStage": "XYStage", "470 AO-TriggerSampleRate": "100000", "PRIME-Timing-PreTriggerDelayNs": "24672000.0000", "528-DA Device": "528 Shutter", "Focus-Description": "Nikon TE2000 focus adapter", "FilterBlock-State": "5", "520 AO-Demo": "No", "COM3-DelayBetweenCharsMs": "0.0000", "470 Shutter-Volts": "0.0000", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "PRIME-PP 2 THRESHOLD": "125", "PRIME-PP 4 THRESHOLD": "75", "XYStage-Acceleration": "4.0000", "470 Shutter-Demo": "No", "PRIME-Name": "PMPCIECam00", "640-DA Device": "640 Shutter", "405 AO-Block voltage": "false", "ElapsedTime-ms": 1400182, "PRIME-Offset": "100", "445 AO-IOChannel": "Dev1/ao1", "640 AO-MaxVolts": "5.0000", "PositionIndex": 34, "PRIME-PP 1 ENABLED": "Yes", "COM1-BaudRate": "9600", "640-State": "0", "COM3-Verbose": "1", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "LudlController-TransmissionDelay": "0", "PRIME-FullWellCapacity": "62000", "PRIME-SMARTStreamingEnabled": "No", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "640 Shutter-Description": "NI DAC", "528-Description": "DA device that is used as a shutter", "COM4-Description": "Serial port driver (boost:asio)", "405 Shutter-Description": "NI DAC", "TE2000-Name": "TE2000", "528 Shutter-Description": "NI DAC", "COM9-Description": "Serial port driver (boost:asio)", "LudlController-Name": "LudlController", "445 Shutter-TriggerSequenceLength": "1000", "PRIME-SerialNumber": "A18B203008", "445 AO-Volts": "0.0000", "PRIME-X-dimension": "1200", "PRIME-ShutterCloseDelay": "0", "XLIGHT Disk slider-State": "2", "Position": "Default", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 AO-IOChannel": "Dev1/ao5", "520 AO-IOChannel": "Dev1/ao4", "528 AO-TriggerSequenceLength": "1000", "405 Shutter-MaxVolts": "5.0000", "470-DA Device": "470 Shutter", "PRIME-FTCapable": "No", "Core-ChannelGroup": "Channels", "555 AO-SupportsTriggering": "0", "PRIME-Y-dimension": "1200", "Frame": 0, "555 Shutter-Sequenceable": "1", "PRIME-Timing-ExposureTimeNs": "499998000.0000", "Core-ImageProcessor": "", "528-State": "0", "520 AO-Block voltage": "false", "XLIGHT Excitation Wheel-Label": "QUAD", "640 Shutter-Demo": "No", "COM3-StopBits": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "520 Shutter-IOChannel": "Dev1/ao11", "Width": 1200, "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-SupportsTriggering": "0", "640 AO-TriggerSampleRate": "100000", "520-Description": "DA device that is used as a shutter", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "405 Shutter-Sequenceable": "1", "405 Shutter-SupportsTriggering": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-TriggerSampleRate": "100000", "640 Shutter-MaxVolts": "5.0000", "555 AO-MaxVolts": "5.0000", "520 AO-TriggerSequenceLength": "1000", "COM3-BaudRate": "9600", "COM9-BaudRate": "9600", "Vincent-D3-Channel #": "Ch. #1", "PRIME-PMode": "Normal", "OpticalPath-Label": "Left(100)", "BitDepth": 16, "555 AO-TriggerSequenceLength": "1000", "445 Shutter-Description": "NI DAC", "405 AO-Sequenceable": "1", "PRIME-AcquisitionMethod": "Callbacks", "555 AO-Name": "AnalogIO", "PRIME-ShutterMode": "Pre-Exposure", "COM3-Handshaking": "Off", "445 AO-SupportsTriggering": "0", "ROI": "0-0-1200-1200", "COM9-Verbose": "1", "PRIME-Color": "OFF", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "PRIME-ShutterOpenDelay": "0", "COM1-Parity": "None", "Slice": 5, "SlicePosition": 0, "COM9-DelayBetweenCharsMs": "0.0000", "555-Name": "DA Shutter", "PIEZO-LudlSingleAxisName": "B", "XLIGHT Emission Wheel-Label": "585/11(T)", "640 Shutter-Name": "AnalogIO", "640 AO-Sequenceable": "1", "520 Shutter-Demo": "No", "528 Shutter-Demo": "No", "470-Name": "DA Shutter", "520-DA Device": "520 Shutter", "XYStage-Description": "Ludl XY stage driver adapter", "445 Shutter-MinVolts": "0.0000", "528 AO-TriggerSampleRate": "100000", "405 AO-MinVolts": "0.0000", "405 Shutter-TriggerSequenceLength": "1000", "Height": 1200, "555 AO-Block voltage": "false", "640 Shutter-Volts": "0.0000", "PixelType": "GRAY16", "445 Shutter-Block voltage": "false", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "520 Shutter-TriggerSequenceLength": "1000", "COM9-Name": "COM9", "Core-SLM": "", "555 AO-Volts": "4.5000", "555 Shutter-Block voltage": "false", "XYStage-Speed": "1800.0000", "520 AO-MaxVolts": "5.0000", "528 Shutter-SupportsTriggering": "0", "COM1-Description": "Serial port driver (boost:asio)", "PRIME-MetadataEnabled": "No", "520 Shutter-MinVolts": "0.0000", "470 Shutter-Sequenceable": "1", "PRIME-PreampDelay": "0", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-MinVolts": "0.0000", "ChannelIndex": 1, "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Core-AutoShutter": "1", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "Exposure-ms": 160, "PRIME-PP 2 ENABLED": "Yes", "640 Shutter-TriggerSampleRate": "100000", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "470 Shutter-IOChannel": "Dev1/ao10", "405 AO-Demo": "No", "PixelSizeUm": 1.1, "470 AO-Demo": "No", "470 Shutter-MaxVolts": "5.0000", "Vincent-D3-Name": "Vincent-D3", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FileName": "img_000000000_Il-T_005.tif", "LudlController-Reset": "Operate", "PRIME-Exposure": "160.00", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "445-Name": "DA Shutter", "555 Shutter-Name": "AnalogIO", "520 Shutter-TriggerSampleRate": "100000", "445 Shutter-IOChannel": "Dev1/ao9", "PRIME-TransposeCorrection": "0", "XYStage-StepSize": "0.1000", "445-Description": "DA device that is used as a shutter", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 AO-IOChannel": "Dev1/ao7", "520 Shutter-Name": "AnalogIO", "COM3-DataBits": "8", "640 AO-Volts": "0.0000", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "555 Shutter-IOChannel": "Dev1/ao13", "WaitInterval": null, "520 Shutter-Sequenceable": "1", "YPositionUm": 545.6, "445-State": "0", "445-DA Device": "445 Shutter", "PRIME-CircularBufferAutoSize": "ON", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "555 AO-TriggerSampleRate": "100000", "445 Shutter-Volts": "0.0000", "XLIGHT Hub-Port": "COM3", "Core-AutoFocus": "", "COM4-Handshaking": "Off", "528 Shutter-MinVolts": "0.0000", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "445 Shutter-Name": "AnalogIO", "528 Shutter-Sequenceable": "1", "XYStage-TransposeMirrorX": "0", "XYStage-TransposeMirrorY": "0", "PRIME-ReadoutRate": "100MHz 16bit", "XYStage-Name": "Stage", "640 AO-SupportsTriggering": "0", "640-Name": "DA Shutter", "640 Shutter-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "640 AO-Block voltage": "false", "445 Shutter-Sequenceable": "1", "470 AO-MaxVolts": "5.0000", "COM4-BaudRate": "9600", "470-Description": "DA device that is used as a shutter", "520 Shutter-Description": "NI DAC", "PRIME-CCDTemperature": "-14.8500", "Time": "2019-12-19 09:59:30 -0500", "640 AO-TriggerSequenceLength": "1000", "470 AO-Name": "AnalogIO", "XLIGHT Disk slider-Label": "Disk Pos 40um", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "Binning": "1x1", "TE2000-ModelType": "1", "COM3-AnswerTimeout": "500.0000", "555 Shutter-SupportsTriggering": "0", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "TE2000-Port": "COM4", "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Description": "NI DAC", "COM4-AnswerTimeout": "500.0000", "COM1-DelayBetweenCharsMs": "0.0000", "FilterBlock-Label": "Confocal", "445 AO-Name": "AnalogIO", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-Timing-ReadoutTimeNs": "24672000.0000", "528 AO-Demo": "No", "520 AO-Sequenceable": "1", "555-Description": "DA device that is used as a shutter", "405-DA Device": "405 Shutter", "405 AO-TriggerSequenceLength": "1000", "640 AO-MinVolts": "0.0000", "640 Shutter-Block voltage": "false", "FrameIndex": 0, "PRIME-Port": "CMOS", "555 AO-MinVolts": "0.0000", "COM3-Description": "Serial port driver (boost:asio)", "528 AO-Description": "NI DAC", "555 AO-IOChannel": "Dev1/ao6", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "555 AO-TriggerInputLine": "/Dev1/PFI0", "PRIME-ActualInterval-ms": "390.5200", "LudlController-Port": "COM9", "445 Shutter-Demo": "No", "PRIME-Trigger-Expose Out-Mux": "1", "SliceIndex": 5, "PRIME-CCDTemperatureSetPoint": "-15.0000", "405 Shutter-MinVolts": "0.0000", "PRIME-ExposeOutMode": "First Row", "AxisPositions": null, "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Volts": "5.0000", "528-Name": "DA Shutter", "555-State": "1", "XLIGHT Dichroic-Label": "QUAD", "445 AO-Sequenceable": "1", "405-Name": "DA Shutter", "405 AO-IOChannel": "Dev1/ao0", "Core-Camera": "PRIME", "Focus-Name": "Focus", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "405 Shutter-IOChannel": "Dev1/ao8", "640 AO-Demo": "No", "640 Shutter-SupportsTriggering": "0", "445 AO-Demo": "No", "555-DA Device": "555 Shutter", "470 Shutter-Description": "NI DAC", "528 AO-Volts": "0.0000", "COM1-DataBits": "8", "Vincent-D3-Address": "x", "520 AO-SupportsTriggering": "0", "405-Description": "DA device that is used as a shutter", "640 Shutter-MinVolts": "0.0000", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "405 AO-Name": "AnalogIO", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "640 AO-TriggerInputLine": "/Dev1/PFI0", "405 AO-TriggerInputLine": "/Dev1/PFI0", "640-Description": "DA device that is used as a shutter", "445 Shutter-TriggerSampleRate": "100000", "470 Shutter-TriggerSequenceLength": "1000", "COM1-StopBits": "1", "520 Shutter-SupportsTriggering": "0", "405-State": "0", "Core-Initialize": "1", "XLIGHT Emission Wheel-State": "3", "520 AO-Description": "NI DAC", "528 AO-TriggerInputLine": "/Dev1/PFI0", "445 Shutter-MaxVolts": "5.0000", "COM9-Handshaking": "Off", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "XLIGHT Spin motor-Label": "On (spinning)", "PRIME-PP 5 ENABLED": "No", "UUID": "b2ee18ce-1523-47a4-9bd7-a00abdcca00d", "PRIME-TriggerMode": "Internal Trigger", "COM1-Handshaking": "Off", "PRIME-Timing-PostTriggerDelayNs": "49344000.0000", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "Focus-Position": "17741.5000", "528 Shutter-Block voltage": "false", "Channel": "Il-T", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "555 Shutter-Demo": "No", "405 Shutter-Demo": "No", "528 AO-Name": "AnalogIO", "520-State": "0", "Core-Galvo": "", "405 Shutter-TriggerSampleRate": "100000", "Focus-FocusStepSize_(nm)": "100", "PRIME-PixelType": "16bit", "COM4-DataBits": "8", "470 AO-IOChannel": "Dev1/ao2", "520 AO-MinVolts": "0.0000", "COM9-Parity": "None", "PRIME-CircularBufferEnabled": "ON", "PRIME-PP 6 Reset": "No", "640 AO-Description": "NI DAC", "Source": "PRIME", "445 AO-MinVolts": "0.0000", "PIEZO-Name": "Stage", "445 AO-Description": "NI DAC", "PRIME-CircularBufferFrameCount": "46", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "520 AO-Volts": "0.0000", "528 Shutter-TriggerSampleRate": "100000", "XPositionUm": -1324.9, "OpticalPath-State": "4", "PRIME-PP 3 THRESHOLD": "80", "PRIME-PVCAM Version": "3.7.1", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Gain": "1-HDR", "640 Shutter-TriggerSequenceLength": "1000", "528 AO-SupportsTriggering": "0", "445 AO-TriggerInputLine": "/Dev1/PFI0", "OpticalPath-Name": "OpticalPath", "PRIME-PP 4 ENABLED": "Yes", "PRIME-PVCAM Adapter Version": "1.3.29", "COM3-Name": "COM3", "405 AO-MaxVolts": "5.0000", "520 Shutter-Block voltage": "false", "PRIME-Timing-ClearingTimeNs": "49344000.0000", "Core-Focus": "PIEZO", "528 AO-Block voltage": "false", "TE2000-Description": "Nikon TE2000 hub", "528 AO-MinVolts": "0.0000", "COM4-Parity": "None", "Summary": { "Slices": 11, "ChContrastMin": [ 0, 0, 0, 0, 0, 0 ], "PixelAspect": 1, "SlicesFirst": true, "Interval_ms": 0, "Time": "2019-12-19 09:59:21 -0500", "Prefix": "seq_13", "InitialPositionList": [ { "Label": "Pos0", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.21], "Focus": [17741.5], "XYStage": [ 44542.200000000004, 31636.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos1", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.22], "Focus": [17741.4], "XYStage": [ 43801.100000000006, 31637.9 ] }, "GridColumnIndex": 0 }, { "Label": "Pos2", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.5], "XYStage": [ 38561.3, 32040.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos3", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.185], "Focus": [17741.3], "XYStage": [ 37698.4, 32041.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos4", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.16], "Focus": [17741.5], "XYStage": [ 32375.4, 31758.9 ] }, "GridColumnIndex": 0 }, { "Label": "Pos5", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.17000000000002], "Focus": [17741.3], "XYStage": [ 31770.7, 31758.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos6", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.4], "XYStage": [ 26536.800000000003, 31068.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos7", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.205], "Focus": [17741.4], "XYStage": [ 25420.2, 31068.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos8", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.85], "Focus": [17741.3], "XYStage": [ 21059, 31508 ] }, "GridColumnIndex": 0 }, { "Label": "Pos9", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.82], "Focus": [17741.3], "XYStage": [ 19731.9, 31506.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos10", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.79], "Focus": [17741.4], "XYStage": [ 14528.5, 32007.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos11", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.83], "Focus": [17741.2], "XYStage": [ 13830.300000000001, 32009.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos12", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.79], "Focus": [17741.3], "XYStage": [ 8775.300000000001, 32017.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos13", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.815], "Focus": [17741.3], "XYStage": [ 8109.200000000001, 32008.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos14", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.805], "Focus": [17741.3], "XYStage": [ 3483.3, 31908.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos15", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.82], "Focus": [17741.2], "XYStage": [ 2686.8, 31903.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos16", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [211.48000000000002], "Focus": [17741.1], "XYStage": [ -1978.9, 31973.600000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos17", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [211.51500000000001], "Focus": [17741.1], "XYStage": [ -2957.8, 31982.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos18", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.195], "Focus": [17741.5], "XYStage": [ 47747.4, -576.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos19", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.23000000000002], "Focus": [17741.6], "XYStage": [ 47053.9, -576.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos20", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.205], "Focus": [17741.4], "XYStage": [ 41871.200000000004, -479.8 ] }, "GridColumnIndex": 0 }, { "Label": "Pos21", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.4], "XYStage": [ 40882.600000000006, -392 ] }, "GridColumnIndex": 0 }, { "Label": "Pos22", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.24], "Focus": [17741.4], "XYStage": [ 35905.6, -135.3 ] }, "GridColumnIndex": 0 }, { "Label": "Pos23", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.195], "Focus": [17741.5], "XYStage": [ 34990.9, -132.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos24", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.46], "Focus": [17741.4], "XYStage": [ 29678.100000000002, -958.1 ] }, "GridColumnIndex": 0 }, { "Label": "Pos25", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.44], "Focus": [17741.5], "XYStage": [ 28983.600000000002, -939.4000000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos26", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.475], "Focus": [17741.5], "XYStage": [ 23019.7, -122 ] }, "GridColumnIndex": 0 }, { "Label": "Pos27", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.44], "Focus": [17741.7], "XYStage": [ 22428, -116.80000000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos28", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.46], "Focus": [17741.7], "XYStage": [ 16364.900000000001, -181.10000000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos29", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.48000000000002], "Focus": [17741.5], "XYStage": [ 15712.1, -180 ] }, "GridColumnIndex": 0 }, { "Label": "Pos30", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.45000000000002], "Focus": [17741.7], "XYStage": [ 10346.1, 77.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos31", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.49], "Focus": [17741.4], "XYStage": [ 9755.5, 85.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos32", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.475], "Focus": [17741.5], "XYStage": [ 4775.5, 293.90000000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos33", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.445], "Focus": [17741.4], "XYStage": [ 3954.9, 293.90000000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos34", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.455], "Focus": [17741.4], "XYStage": [ -1324.9, 545.6 ] }, "GridColumnIndex": 0 }, { "Label": "Pos35", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.445], "Focus": [17741.3], "XYStage": [ -2097, 576.6 ] }, "GridColumnIndex": 0 } ], "z-step_um": 10, "GridRow": 0, "BitDepth": 16, "Depth": 2, "Channels": 6, "Source": "Micro-Manager", "ChColors": [ -16711681, -256, -65281, -1, -1, -16776961 ], "MetadataVersion": 10, "KeepShutterOpenChannels": true, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536, 65536 ], "CustomIntervals_ms": [], "Height": 1200, "MicroManagerVersion": "1.4.23 20180219", "GridColumn": 0, "UUID": "18808e7c-f2e6-47b3-90d8-1d779ce87dbd", "PixelType": "GRAY16", "Width": 1200, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\Yan\\20191218-YC113-S7S8-miseq", "Frames": 1, "Comment": "", "UserName": "Nikon", "PixelSize_um": 1.1, "ROI": [ 0, 0, 1200, 1200 ], "Date": "2019-12-19", "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC", "P-DAPI-high" ], "KeepShutterOpenSlices": true, "IJType": 1, "ComputerName": "ZadorConfocal2", "Positions": 36, "PositionIndex": 34, "CameraTimeout": 20000, "TimeFirst": false }, "640 AO-Name": "AnalogIO", "Vincent-D3-Command": "Close" }, "FrameKey-0-1-6": { "COM4-StopBits": "1", "528 AO-MaxVolts": "5.0000", "XLIGHT Excitation Wheel-State": "1", "470 Shutter-Name": "AnalogIO", "Core-Shutter": "555", "555 AO-Demo": "No", "XLIGHT Dichroic-State": "2", "XYStage-StepSize-Y": "0.1000", "ZPositionUm": 228.455, "XYStage-StepSize-X": "0.1000", "PRIME-FirmwareVersion": "20.28", "PRIME-ClearCycles": "2", "520-Name": "DA Shutter", "405 Shutter-Name": "AnalogIO", "PIEZO-StepSize": "0.0050", "PRIME-ClearMode": "Pre-Exposure", "470 AO-MinVolts": "0.0000", "405 AO-SupportsTriggering": "1", "CameraChannelIndex": 0, "XYStage-StartSpeed": "500.0000", "COM9-StopBits": "2", "405 AO-Description": "NI DAC", "COM4-DelayBetweenCharsMs": "0.0000", "445 AO-Block voltage": "false", "520 AO-TriggerInputLine": "/Dev1/PFI0", "555 AO-Description": "NI DAC", "520 AO-TriggerSampleRate": "100000", "COM4-Verbose": "1", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM4-Name": "COM4", "405 Shutter-Volts": "0.0000", "Vincent-D3-Port": "COM1", "PRIME-PP 4 MIN ADU AFFECTED": "200", "470 Shutter-MinVolts": "0.0000", "PRIME-PP 3 ENABLED": "Yes", "470 AO-Description": "NI DAC", "PRIME-Description": "PVCAM API device adapter", "520 Shutter-MaxVolts": "5.0000", "520 Shutter-Volts": "0.0000", "528 AO-Sequenceable": "1", "PRIME-Binning": "1x1", "555 Shutter-MaxVolts": "5.0000", "528 Shutter-TriggerSequenceLength": "1000", "405 Shutter-Block voltage": "false", "XLIGHT Spin motor-State": "1", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "Vincent-D3-Time to open (ms)": "35.0000", "Vincent-D3-Time to close (ms)": "35.0000", "445 AO-TriggerSampleRate": "100000", "640 Shutter-IOChannel": "Dev1/ao14", "555 AO-Sequenceable": "1", "520 AO-Name": "AnalogIO", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "470 AO-Block voltage": "false", "COM1-Verbose": "1", "COM9-AnswerTimeout": "10000.0000", "528 Shutter-Name": "AnalogIO", "PRIME-Trigger Timeout (secs)": "10", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "NextFrame": 0, "470 AO-TriggerSequenceLength": "1000", "PRIME-TransposeXY": "0", "445 AO-TriggerSequenceLength": "1000", "COM1-Name": "COM1", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "COM9-DataBits": "8", "COM1-AnswerTimeout": "500.0000", "Camera": "", "TE2000-Version": "V2.50-V2.50-V2.10", "528 Shutter-Volts": "0.0000", "XLIGHT Hub-Name": "XLIGHT Hub", "PIEZO-Description": "Ludl stage driver adapter", "PositionName": "Pos34", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSampleRate": "100000", "PRIME-FanSpeedSetpoint": "High", "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "405 AO-TriggerSampleRate": "100000", "470 Shutter-Block voltage": "false", "470-State": "0", "COM3-Parity": "None", "405 AO-Volts": "0.0000", "PRIME-TransposeMirrorY": "0", "PRIME-ChipName": "GS144BSI", "470 AO-Volts": "0.0000", "PRIME-TransposeMirrorX": "0", "445 Shutter-SupportsTriggering": "0", "470 Shutter-SupportsTriggering": "0", "XLIGHT Hub-Description": "XLIGHT hub", "PRIME-PreampOffLimit": "10000", "470 AO-Sequenceable": "1", "528 Shutter-MaxVolts": "5.0000", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "Core-XYStage": "XYStage", "470 AO-TriggerSampleRate": "100000", "PRIME-Timing-PreTriggerDelayNs": "24672000.0000", "528-DA Device": "528 Shutter", "Focus-Description": "Nikon TE2000 focus adapter", "FilterBlock-State": "5", "520 AO-Demo": "No", "COM3-DelayBetweenCharsMs": "0.0000", "470 Shutter-Volts": "0.0000", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "PRIME-PP 2 THRESHOLD": "125", "PRIME-PP 4 THRESHOLD": "75", "XYStage-Acceleration": "4.0000", "470 Shutter-Demo": "No", "PRIME-Name": "PMPCIECam00", "640-DA Device": "640 Shutter", "405 AO-Block voltage": "false", "ElapsedTime-ms": 1400584, "PRIME-Offset": "100", "445 AO-IOChannel": "Dev1/ao1", "640 AO-MaxVolts": "5.0000", "PositionIndex": 34, "PRIME-PP 1 ENABLED": "Yes", "COM1-BaudRate": "9600", "640-State": "0", "COM3-Verbose": "1", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "LudlController-TransmissionDelay": "0", "PRIME-FullWellCapacity": "62000", "PRIME-SMARTStreamingEnabled": "No", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "640 Shutter-Description": "NI DAC", "528-Description": "DA device that is used as a shutter", "COM4-Description": "Serial port driver (boost:asio)", "405 Shutter-Description": "NI DAC", "TE2000-Name": "TE2000", "528 Shutter-Description": "NI DAC", "COM9-Description": "Serial port driver (boost:asio)", "LudlController-Name": "LudlController", "445 Shutter-TriggerSequenceLength": "1000", "PRIME-SerialNumber": "A18B203008", "445 AO-Volts": "0.0000", "PRIME-X-dimension": "1200", "PRIME-ShutterCloseDelay": "0", "XLIGHT Disk slider-State": "2", "Position": "Default", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 AO-IOChannel": "Dev1/ao5", "520 AO-IOChannel": "Dev1/ao4", "528 AO-TriggerSequenceLength": "1000", "405 Shutter-MaxVolts": "5.0000", "470-DA Device": "470 Shutter", "PRIME-FTCapable": "No", "Core-ChannelGroup": "Channels", "555 AO-SupportsTriggering": "0", "PRIME-Y-dimension": "1200", "Frame": 0, "555 Shutter-Sequenceable": "1", "PRIME-Timing-ExposureTimeNs": "499998000.0000", "Core-ImageProcessor": "", "528-State": "0", "520 AO-Block voltage": "false", "XLIGHT Excitation Wheel-Label": "QUAD", "640 Shutter-Demo": "No", "COM3-StopBits": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "520 Shutter-IOChannel": "Dev1/ao11", "Width": 1200, "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-SupportsTriggering": "0", "640 AO-TriggerSampleRate": "100000", "520-Description": "DA device that is used as a shutter", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "405 Shutter-Sequenceable": "1", "405 Shutter-SupportsTriggering": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-TriggerSampleRate": "100000", "640 Shutter-MaxVolts": "5.0000", "555 AO-MaxVolts": "5.0000", "520 AO-TriggerSequenceLength": "1000", "COM3-BaudRate": "9600", "COM9-BaudRate": "9600", "Vincent-D3-Channel #": "Ch. #1", "PRIME-PMode": "Normal", "OpticalPath-Label": "Left(100)", "BitDepth": 16, "555 AO-TriggerSequenceLength": "1000", "445 Shutter-Description": "NI DAC", "405 AO-Sequenceable": "1", "PRIME-AcquisitionMethod": "Callbacks", "555 AO-Name": "AnalogIO", "PRIME-ShutterMode": "Pre-Exposure", "COM3-Handshaking": "Off", "445 AO-SupportsTriggering": "0", "ROI": "0-0-1200-1200", "COM9-Verbose": "1", "PRIME-Color": "OFF", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "PRIME-ShutterOpenDelay": "0", "COM1-Parity": "None", "Slice": 6, "SlicePosition": 10, "COM9-DelayBetweenCharsMs": "0.0000", "555-Name": "DA Shutter", "PIEZO-LudlSingleAxisName": "B", "XLIGHT Emission Wheel-Label": "585/11(T)", "640 Shutter-Name": "AnalogIO", "640 AO-Sequenceable": "1", "520 Shutter-Demo": "No", "528 Shutter-Demo": "No", "470-Name": "DA Shutter", "520-DA Device": "520 Shutter", "XYStage-Description": "Ludl XY stage driver adapter", "445 Shutter-MinVolts": "0.0000", "528 AO-TriggerSampleRate": "100000", "405 AO-MinVolts": "0.0000", "405 Shutter-TriggerSequenceLength": "1000", "Height": 1200, "555 AO-Block voltage": "false", "640 Shutter-Volts": "0.0000", "PixelType": "GRAY16", "445 Shutter-Block voltage": "false", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "520 Shutter-TriggerSequenceLength": "1000", "COM9-Name": "COM9", "Core-SLM": "", "555 AO-Volts": "4.5000", "555 Shutter-Block voltage": "false", "XYStage-Speed": "1800.0000", "520 AO-MaxVolts": "5.0000", "528 Shutter-SupportsTriggering": "0", "COM1-Description": "Serial port driver (boost:asio)", "PRIME-MetadataEnabled": "No", "520 Shutter-MinVolts": "0.0000", "470 Shutter-Sequenceable": "1", "PRIME-PreampDelay": "0", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-MinVolts": "0.0000", "ChannelIndex": 1, "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Core-AutoShutter": "1", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "Exposure-ms": 160, "PRIME-PP 2 ENABLED": "Yes", "640 Shutter-TriggerSampleRate": "100000", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "470 Shutter-IOChannel": "Dev1/ao10", "405 AO-Demo": "No", "PixelSizeUm": 1.1, "470 AO-Demo": "No", "470 Shutter-MaxVolts": "5.0000", "Vincent-D3-Name": "Vincent-D3", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FileName": "img_000000000_Il-T_006.tif", "LudlController-Reset": "Operate", "PRIME-Exposure": "160.00", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "445-Name": "DA Shutter", "555 Shutter-Name": "AnalogIO", "520 Shutter-TriggerSampleRate": "100000", "445 Shutter-IOChannel": "Dev1/ao9", "PRIME-TransposeCorrection": "0", "XYStage-StepSize": "0.1000", "445-Description": "DA device that is used as a shutter", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 AO-IOChannel": "Dev1/ao7", "520 Shutter-Name": "AnalogIO", "COM3-DataBits": "8", "640 AO-Volts": "0.0000", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "555 Shutter-IOChannel": "Dev1/ao13", "WaitInterval": null, "520 Shutter-Sequenceable": "1", "YPositionUm": 545.6, "445-State": "0", "445-DA Device": "445 Shutter", "PRIME-CircularBufferAutoSize": "ON", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "555 AO-TriggerSampleRate": "100000", "445 Shutter-Volts": "0.0000", "XLIGHT Hub-Port": "COM3", "Core-AutoFocus": "", "COM4-Handshaking": "Off", "528 Shutter-MinVolts": "0.0000", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "445 Shutter-Name": "AnalogIO", "528 Shutter-Sequenceable": "1", "XYStage-TransposeMirrorX": "0", "XYStage-TransposeMirrorY": "0", "PRIME-ReadoutRate": "100MHz 16bit", "XYStage-Name": "Stage", "640 AO-SupportsTriggering": "0", "640-Name": "DA Shutter", "640 Shutter-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "640 AO-Block voltage": "false", "445 Shutter-Sequenceable": "1", "470 AO-MaxVolts": "5.0000", "COM4-BaudRate": "9600", "470-Description": "DA device that is used as a shutter", "520 Shutter-Description": "NI DAC", "PRIME-CCDTemperature": "-14.8500", "Time": "2019-12-19 09:59:30 -0500", "640 AO-TriggerSequenceLength": "1000", "470 AO-Name": "AnalogIO", "XLIGHT Disk slider-Label": "Disk Pos 40um", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "Binning": "1x1", "TE2000-ModelType": "1", "COM3-AnswerTimeout": "500.0000", "555 Shutter-SupportsTriggering": "0", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "TE2000-Port": "COM4", "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Description": "NI DAC", "COM4-AnswerTimeout": "500.0000", "COM1-DelayBetweenCharsMs": "0.0000", "FilterBlock-Label": "Confocal", "445 AO-Name": "AnalogIO", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-Timing-ReadoutTimeNs": "24672000.0000", "528 AO-Demo": "No", "520 AO-Sequenceable": "1", "555-Description": "DA device that is used as a shutter", "405-DA Device": "405 Shutter", "405 AO-TriggerSequenceLength": "1000", "640 AO-MinVolts": "0.0000", "640 Shutter-Block voltage": "false", "FrameIndex": 0, "PRIME-Port": "CMOS", "555 AO-MinVolts": "0.0000", "COM3-Description": "Serial port driver (boost:asio)", "528 AO-Description": "NI DAC", "555 AO-IOChannel": "Dev1/ao6", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "555 AO-TriggerInputLine": "/Dev1/PFI0", "PRIME-ActualInterval-ms": "390.5200", "LudlController-Port": "COM9", "445 Shutter-Demo": "No", "PRIME-Trigger-Expose Out-Mux": "1", "SliceIndex": 6, "PRIME-CCDTemperatureSetPoint": "-15.0000", "405 Shutter-MinVolts": "0.0000", "PRIME-ExposeOutMode": "First Row", "AxisPositions": null, "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Volts": "5.0000", "528-Name": "DA Shutter", "555-State": "1", "XLIGHT Dichroic-Label": "QUAD", "445 AO-Sequenceable": "1", "405-Name": "DA Shutter", "405 AO-IOChannel": "Dev1/ao0", "Core-Camera": "PRIME", "Focus-Name": "Focus", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "405 Shutter-IOChannel": "Dev1/ao8", "640 AO-Demo": "No", "640 Shutter-SupportsTriggering": "0", "445 AO-Demo": "No", "555-DA Device": "555 Shutter", "470 Shutter-Description": "NI DAC", "528 AO-Volts": "0.0000", "COM1-DataBits": "8", "Vincent-D3-Address": "x", "520 AO-SupportsTriggering": "0", "405-Description": "DA device that is used as a shutter", "640 Shutter-MinVolts": "0.0000", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "405 AO-Name": "AnalogIO", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "640 AO-TriggerInputLine": "/Dev1/PFI0", "405 AO-TriggerInputLine": "/Dev1/PFI0", "640-Description": "DA device that is used as a shutter", "445 Shutter-TriggerSampleRate": "100000", "470 Shutter-TriggerSequenceLength": "1000", "COM1-StopBits": "1", "520 Shutter-SupportsTriggering": "0", "405-State": "0", "Core-Initialize": "1", "XLIGHT Emission Wheel-State": "3", "520 AO-Description": "NI DAC", "528 AO-TriggerInputLine": "/Dev1/PFI0", "445 Shutter-MaxVolts": "5.0000", "COM9-Handshaking": "Off", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "XLIGHT Spin motor-Label": "On (spinning)", "PRIME-PP 5 ENABLED": "No", "UUID": "48f92296-eef0-4d4b-8698-f301d39b12d9", "PRIME-TriggerMode": "Internal Trigger", "COM1-Handshaking": "Off", "PRIME-Timing-PostTriggerDelayNs": "49344000.0000", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "Focus-Position": "17741.5000", "528 Shutter-Block voltage": "false", "Channel": "Il-T", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "555 Shutter-Demo": "No", "405 Shutter-Demo": "No", "528 AO-Name": "AnalogIO", "520-State": "0", "Core-Galvo": "", "405 Shutter-TriggerSampleRate": "100000", "Focus-FocusStepSize_(nm)": "100", "PRIME-PixelType": "16bit", "COM4-DataBits": "8", "470 AO-IOChannel": "Dev1/ao2", "520 AO-MinVolts": "0.0000", "COM9-Parity": "None", "PRIME-CircularBufferEnabled": "ON", "PRIME-PP 6 Reset": "No", "640 AO-Description": "NI DAC", "Source": "PRIME", "445 AO-MinVolts": "0.0000", "PIEZO-Name": "Stage", "445 AO-Description": "NI DAC", "PRIME-CircularBufferFrameCount": "46", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "520 AO-Volts": "0.0000", "528 Shutter-TriggerSampleRate": "100000", "XPositionUm": -1324.9, "OpticalPath-State": "4", "PRIME-PP 3 THRESHOLD": "80", "PRIME-PVCAM Version": "3.7.1", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Gain": "1-HDR", "640 Shutter-TriggerSequenceLength": "1000", "528 AO-SupportsTriggering": "0", "445 AO-TriggerInputLine": "/Dev1/PFI0", "OpticalPath-Name": "OpticalPath", "PRIME-PP 4 ENABLED": "Yes", "PRIME-PVCAM Adapter Version": "1.3.29", "COM3-Name": "COM3", "405 AO-MaxVolts": "5.0000", "520 Shutter-Block voltage": "false", "PRIME-Timing-ClearingTimeNs": "49344000.0000", "Core-Focus": "PIEZO", "528 AO-Block voltage": "false", "TE2000-Description": "Nikon TE2000 hub", "528 AO-MinVolts": "0.0000", "COM4-Parity": "None", "Summary": { "Slices": 11, "ChContrastMin": [ 0, 0, 0, 0, 0, 0 ], "PixelAspect": 1, "SlicesFirst": true, "Interval_ms": 0, "Time": "2019-12-19 09:59:21 -0500", "Prefix": "seq_13", "InitialPositionList": [ { "Label": "Pos0", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.21], "Focus": [17741.5], "XYStage": [ 44542.200000000004, 31636.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos1", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.22], "Focus": [17741.4], "XYStage": [ 43801.100000000006, 31637.9 ] }, "GridColumnIndex": 0 }, { "Label": "Pos2", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.5], "XYStage": [ 38561.3, 32040.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos3", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.185], "Focus": [17741.3], "XYStage": [ 37698.4, 32041.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos4", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.16], "Focus": [17741.5], "XYStage": [ 32375.4, 31758.9 ] }, "GridColumnIndex": 0 }, { "Label": "Pos5", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.17000000000002], "Focus": [17741.3], "XYStage": [ 31770.7, 31758.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos6", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.4], "XYStage": [ 26536.800000000003, 31068.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos7", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.205], "Focus": [17741.4], "XYStage": [ 25420.2, 31068.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos8", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.85], "Focus": [17741.3], "XYStage": [ 21059, 31508 ] }, "GridColumnIndex": 0 }, { "Label": "Pos9", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.82], "Focus": [17741.3], "XYStage": [ 19731.9, 31506.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos10", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.79], "Focus": [17741.4], "XYStage": [ 14528.5, 32007.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos11", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.83], "Focus": [17741.2], "XYStage": [ 13830.300000000001, 32009.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos12", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.79], "Focus": [17741.3], "XYStage": [ 8775.300000000001, 32017.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos13", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.815], "Focus": [17741.3], "XYStage": [ 8109.200000000001, 32008.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos14", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.805], "Focus": [17741.3], "XYStage": [ 3483.3, 31908.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos15", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.82], "Focus": [17741.2], "XYStage": [ 2686.8, 31903.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos16", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [211.48000000000002], "Focus": [17741.1], "XYStage": [ -1978.9, 31973.600000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos17", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [211.51500000000001], "Focus": [17741.1], "XYStage": [ -2957.8, 31982.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos18", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.195], "Focus": [17741.5], "XYStage": [ 47747.4, -576.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos19", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.23000000000002], "Focus": [17741.6], "XYStage": [ 47053.9, -576.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos20", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.205], "Focus": [17741.4], "XYStage": [ 41871.200000000004, -479.8 ] }, "GridColumnIndex": 0 }, { "Label": "Pos21", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.4], "XYStage": [ 40882.600000000006, -392 ] }, "GridColumnIndex": 0 }, { "Label": "Pos22", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.24], "Focus": [17741.4], "XYStage": [ 35905.6, -135.3 ] }, "GridColumnIndex": 0 }, { "Label": "Pos23", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.195], "Focus": [17741.5], "XYStage": [ 34990.9, -132.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos24", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.46], "Focus": [17741.4], "XYStage": [ 29678.100000000002, -958.1 ] }, "GridColumnIndex": 0 }, { "Label": "Pos25", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.44], "Focus": [17741.5], "XYStage": [ 28983.600000000002, -939.4000000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos26", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.475], "Focus": [17741.5], "XYStage": [ 23019.7, -122 ] }, "GridColumnIndex": 0 }, { "Label": "Pos27", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.44], "Focus": [17741.7], "XYStage": [ 22428, -116.80000000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos28", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.46], "Focus": [17741.7], "XYStage": [ 16364.900000000001, -181.10000000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos29", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.48000000000002], "Focus": [17741.5], "XYStage": [ 15712.1, -180 ] }, "GridColumnIndex": 0 }, { "Label": "Pos30", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.45000000000002], "Focus": [17741.7], "XYStage": [ 10346.1, 77.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos31", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.49], "Focus": [17741.4], "XYStage": [ 9755.5, 85.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos32", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.475], "Focus": [17741.5], "XYStage": [ 4775.5, 293.90000000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos33", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.445], "Focus": [17741.4], "XYStage": [ 3954.9, 293.90000000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos34", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.455], "Focus": [17741.4], "XYStage": [ -1324.9, 545.6 ] }, "GridColumnIndex": 0 }, { "Label": "Pos35", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.445], "Focus": [17741.3], "XYStage": [ -2097, 576.6 ] }, "GridColumnIndex": 0 } ], "z-step_um": 10, "GridRow": 0, "BitDepth": 16, "Depth": 2, "Channels": 6, "Source": "Micro-Manager", "ChColors": [ -16711681, -256, -65281, -1, -1, -16776961 ], "MetadataVersion": 10, "KeepShutterOpenChannels": true, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536, 65536 ], "CustomIntervals_ms": [], "Height": 1200, "MicroManagerVersion": "1.4.23 20180219", "GridColumn": 0, "UUID": "18808e7c-f2e6-47b3-90d8-1d779ce87dbd", "PixelType": "GRAY16", "Width": 1200, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\Yan\\20191218-YC113-S7S8-miseq", "Frames": 1, "Comment": "", "UserName": "Nikon", "PixelSize_um": 1.1, "ROI": [ 0, 0, 1200, 1200 ], "Date": "2019-12-19", "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC", "P-DAPI-high" ], "KeepShutterOpenSlices": true, "IJType": 1, "ComputerName": "ZadorConfocal2", "Positions": 36, "PositionIndex": 34, "CameraTimeout": 20000, "TimeFirst": false }, "640 AO-Name": "AnalogIO", "Vincent-D3-Command": "Close" }, "FrameKey-0-1-7": { "COM4-StopBits": "1", "528 AO-MaxVolts": "5.0000", "XLIGHT Excitation Wheel-State": "1", "470 Shutter-Name": "AnalogIO", "Core-Shutter": "555", "555 AO-Demo": "No", "XLIGHT Dichroic-State": "2", "XYStage-StepSize-Y": "0.1000", "ZPositionUm": 238.455, "XYStage-StepSize-X": "0.1000", "PRIME-FirmwareVersion": "20.28", "PRIME-ClearCycles": "2", "520-Name": "DA Shutter", "405 Shutter-Name": "AnalogIO", "PIEZO-StepSize": "0.0050", "PRIME-ClearMode": "Pre-Exposure", "470 AO-MinVolts": "0.0000", "405 AO-SupportsTriggering": "1", "CameraChannelIndex": 0, "XYStage-StartSpeed": "500.0000", "COM9-StopBits": "2", "405 AO-Description": "NI DAC", "COM4-DelayBetweenCharsMs": "0.0000", "445 AO-Block voltage": "false", "520 AO-TriggerInputLine": "/Dev1/PFI0", "555 AO-Description": "NI DAC", "520 AO-TriggerSampleRate": "100000", "COM4-Verbose": "1", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM4-Name": "COM4", "405 Shutter-Volts": "0.0000", "Vincent-D3-Port": "COM1", "PRIME-PP 4 MIN ADU AFFECTED": "200", "470 Shutter-MinVolts": "0.0000", "PRIME-PP 3 ENABLED": "Yes", "470 AO-Description": "NI DAC", "PRIME-Description": "PVCAM API device adapter", "520 Shutter-MaxVolts": "5.0000", "520 Shutter-Volts": "0.0000", "528 AO-Sequenceable": "1", "PRIME-Binning": "1x1", "555 Shutter-MaxVolts": "5.0000", "528 Shutter-TriggerSequenceLength": "1000", "405 Shutter-Block voltage": "false", "XLIGHT Spin motor-State": "1", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "Vincent-D3-Time to open (ms)": "35.0000", "Vincent-D3-Time to close (ms)": "35.0000", "445 AO-TriggerSampleRate": "100000", "640 Shutter-IOChannel": "Dev1/ao14", "555 AO-Sequenceable": "1", "520 AO-Name": "AnalogIO", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "470 AO-Block voltage": "false", "COM1-Verbose": "1", "COM9-AnswerTimeout": "10000.0000", "528 Shutter-Name": "AnalogIO", "PRIME-Trigger Timeout (secs)": "10", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "NextFrame": 0, "470 AO-TriggerSequenceLength": "1000", "PRIME-TransposeXY": "0", "445 AO-TriggerSequenceLength": "1000", "COM1-Name": "COM1", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "COM9-DataBits": "8", "COM1-AnswerTimeout": "500.0000", "Camera": "", "TE2000-Version": "V2.50-V2.50-V2.10", "528 Shutter-Volts": "0.0000", "XLIGHT Hub-Name": "XLIGHT Hub", "PIEZO-Description": "Ludl stage driver adapter", "PositionName": "Pos34", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSampleRate": "100000", "PRIME-FanSpeedSetpoint": "High", "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "405 AO-TriggerSampleRate": "100000", "470 Shutter-Block voltage": "false", "470-State": "0", "COM3-Parity": "None", "405 AO-Volts": "0.0000", "PRIME-TransposeMirrorY": "0", "PRIME-ChipName": "GS144BSI", "470 AO-Volts": "0.0000", "PRIME-TransposeMirrorX": "0", "445 Shutter-SupportsTriggering": "0", "470 Shutter-SupportsTriggering": "0", "XLIGHT Hub-Description": "XLIGHT hub", "PRIME-PreampOffLimit": "10000", "470 AO-Sequenceable": "1", "528 Shutter-MaxVolts": "5.0000", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "Core-XYStage": "XYStage", "470 AO-TriggerSampleRate": "100000", "PRIME-Timing-PreTriggerDelayNs": "24672000.0000", "528-DA Device": "528 Shutter", "Focus-Description": "Nikon TE2000 focus adapter", "FilterBlock-State": "5", "520 AO-Demo": "No", "COM3-DelayBetweenCharsMs": "0.0000", "470 Shutter-Volts": "0.0000", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "PRIME-PP 2 THRESHOLD": "125", "PRIME-PP 4 THRESHOLD": "75", "XYStage-Acceleration": "4.0000", "470 Shutter-Demo": "No", "PRIME-Name": "PMPCIECam00", "640-DA Device": "640 Shutter", "405 AO-Block voltage": "false", "ElapsedTime-ms": 1400986, "PRIME-Offset": "100", "445 AO-IOChannel": "Dev1/ao1", "640 AO-MaxVolts": "5.0000", "PositionIndex": 34, "PRIME-PP 1 ENABLED": "Yes", "COM1-BaudRate": "9600", "640-State": "0", "COM3-Verbose": "1", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "LudlController-TransmissionDelay": "0", "PRIME-FullWellCapacity": "62000", "PRIME-SMARTStreamingEnabled": "No", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "640 Shutter-Description": "NI DAC", "528-Description": "DA device that is used as a shutter", "COM4-Description": "Serial port driver (boost:asio)", "405 Shutter-Description": "NI DAC", "TE2000-Name": "TE2000", "528 Shutter-Description": "NI DAC", "COM9-Description": "Serial port driver (boost:asio)", "LudlController-Name": "LudlController", "445 Shutter-TriggerSequenceLength": "1000", "PRIME-SerialNumber": "A18B203008", "445 AO-Volts": "0.0000", "PRIME-X-dimension": "1200", "PRIME-ShutterCloseDelay": "0", "XLIGHT Disk slider-State": "2", "Position": "Default", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 AO-IOChannel": "Dev1/ao5", "520 AO-IOChannel": "Dev1/ao4", "528 AO-TriggerSequenceLength": "1000", "405 Shutter-MaxVolts": "5.0000", "470-DA Device": "470 Shutter", "PRIME-FTCapable": "No", "Core-ChannelGroup": "Channels", "555 AO-SupportsTriggering": "0", "PRIME-Y-dimension": "1200", "Frame": 0, "555 Shutter-Sequenceable": "1", "PRIME-Timing-ExposureTimeNs": "499998000.0000", "Core-ImageProcessor": "", "528-State": "0", "520 AO-Block voltage": "false", "XLIGHT Excitation Wheel-Label": "QUAD", "640 Shutter-Demo": "No", "COM3-StopBits": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "520 Shutter-IOChannel": "Dev1/ao11", "Width": 1200, "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-SupportsTriggering": "0", "640 AO-TriggerSampleRate": "100000", "520-Description": "DA device that is used as a shutter", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "405 Shutter-Sequenceable": "1", "405 Shutter-SupportsTriggering": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-TriggerSampleRate": "100000", "640 Shutter-MaxVolts": "5.0000", "555 AO-MaxVolts": "5.0000", "520 AO-TriggerSequenceLength": "1000", "COM3-BaudRate": "9600", "COM9-BaudRate": "9600", "Vincent-D3-Channel #": "Ch. #1", "PRIME-PMode": "Normal", "OpticalPath-Label": "Left(100)", "BitDepth": 16, "555 AO-TriggerSequenceLength": "1000", "445 Shutter-Description": "NI DAC", "405 AO-Sequenceable": "1", "PRIME-AcquisitionMethod": "Callbacks", "555 AO-Name": "AnalogIO", "PRIME-ShutterMode": "Pre-Exposure", "COM3-Handshaking": "Off", "445 AO-SupportsTriggering": "0", "ROI": "0-0-1200-1200", "COM9-Verbose": "1", "PRIME-Color": "OFF", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "PRIME-ShutterOpenDelay": "0", "COM1-Parity": "None", "Slice": 7, "SlicePosition": 20, "COM9-DelayBetweenCharsMs": "0.0000", "555-Name": "DA Shutter", "PIEZO-LudlSingleAxisName": "B", "XLIGHT Emission Wheel-Label": "585/11(T)", "640 Shutter-Name": "AnalogIO", "640 AO-Sequenceable": "1", "520 Shutter-Demo": "No", "528 Shutter-Demo": "No", "470-Name": "DA Shutter", "520-DA Device": "520 Shutter", "XYStage-Description": "Ludl XY stage driver adapter", "445 Shutter-MinVolts": "0.0000", "528 AO-TriggerSampleRate": "100000", "405 AO-MinVolts": "0.0000", "405 Shutter-TriggerSequenceLength": "1000", "Height": 1200, "555 AO-Block voltage": "false", "640 Shutter-Volts": "0.0000", "PixelType": "GRAY16", "445 Shutter-Block voltage": "false", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "520 Shutter-TriggerSequenceLength": "1000", "COM9-Name": "COM9", "Core-SLM": "", "555 AO-Volts": "4.5000", "555 Shutter-Block voltage": "false", "XYStage-Speed": "1800.0000", "520 AO-MaxVolts": "5.0000", "528 Shutter-SupportsTriggering": "0", "COM1-Description": "Serial port driver (boost:asio)", "PRIME-MetadataEnabled": "No", "520 Shutter-MinVolts": "0.0000", "470 Shutter-Sequenceable": "1", "PRIME-PreampDelay": "0", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-MinVolts": "0.0000", "ChannelIndex": 1, "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Core-AutoShutter": "1", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "Exposure-ms": 160, "PRIME-PP 2 ENABLED": "Yes", "640 Shutter-TriggerSampleRate": "100000", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "470 Shutter-IOChannel": "Dev1/ao10", "405 AO-Demo": "No", "PixelSizeUm": 1.1, "470 AO-Demo": "No", "470 Shutter-MaxVolts": "5.0000", "Vincent-D3-Name": "Vincent-D3", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FileName": "img_000000000_Il-T_007.tif", "LudlController-Reset": "Operate", "PRIME-Exposure": "160.00", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "445-Name": "DA Shutter", "555 Shutter-Name": "AnalogIO", "520 Shutter-TriggerSampleRate": "100000", "445 Shutter-IOChannel": "Dev1/ao9", "PRIME-TransposeCorrection": "0", "XYStage-StepSize": "0.1000", "445-Description": "DA device that is used as a shutter", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 AO-IOChannel": "Dev1/ao7", "520 Shutter-Name": "AnalogIO", "COM3-DataBits": "8", "640 AO-Volts": "0.0000", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "555 Shutter-IOChannel": "Dev1/ao13", "WaitInterval": null, "520 Shutter-Sequenceable": "1", "YPositionUm": 545.6, "445-State": "0", "445-DA Device": "445 Shutter", "PRIME-CircularBufferAutoSize": "ON", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "555 AO-TriggerSampleRate": "100000", "445 Shutter-Volts": "0.0000", "XLIGHT Hub-Port": "COM3", "Core-AutoFocus": "", "COM4-Handshaking": "Off", "528 Shutter-MinVolts": "0.0000", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "445 Shutter-Name": "AnalogIO", "528 Shutter-Sequenceable": "1", "XYStage-TransposeMirrorX": "0", "XYStage-TransposeMirrorY": "0", "PRIME-ReadoutRate": "100MHz 16bit", "XYStage-Name": "Stage", "640 AO-SupportsTriggering": "0", "640-Name": "DA Shutter", "640 Shutter-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "640 AO-Block voltage": "false", "445 Shutter-Sequenceable": "1", "470 AO-MaxVolts": "5.0000", "COM4-BaudRate": "9600", "470-Description": "DA device that is used as a shutter", "520 Shutter-Description": "NI DAC", "PRIME-CCDTemperature": "-14.8500", "Time": "2019-12-19 09:59:31 -0500", "640 AO-TriggerSequenceLength": "1000", "470 AO-Name": "AnalogIO", "XLIGHT Disk slider-Label": "Disk Pos 40um", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "Binning": "1x1", "TE2000-ModelType": "1", "COM3-AnswerTimeout": "500.0000", "555 Shutter-SupportsTriggering": "0", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "TE2000-Port": "COM4", "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Description": "NI DAC", "COM4-AnswerTimeout": "500.0000", "COM1-DelayBetweenCharsMs": "0.0000", "FilterBlock-Label": "Confocal", "445 AO-Name": "AnalogIO", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-Timing-ReadoutTimeNs": "24672000.0000", "528 AO-Demo": "No", "520 AO-Sequenceable": "1", "555-Description": "DA device that is used as a shutter", "405-DA Device": "405 Shutter", "405 AO-TriggerSequenceLength": "1000", "640 AO-MinVolts": "0.0000", "640 Shutter-Block voltage": "false", "FrameIndex": 0, "PRIME-Port": "CMOS", "555 AO-MinVolts": "0.0000", "COM3-Description": "Serial port driver (boost:asio)", "528 AO-Description": "NI DAC", "555 AO-IOChannel": "Dev1/ao6", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "555 AO-TriggerInputLine": "/Dev1/PFI0", "PRIME-ActualInterval-ms": "390.5200", "LudlController-Port": "COM9", "445 Shutter-Demo": "No", "PRIME-Trigger-Expose Out-Mux": "1", "SliceIndex": 7, "PRIME-CCDTemperatureSetPoint": "-15.0000", "405 Shutter-MinVolts": "0.0000", "PRIME-ExposeOutMode": "First Row", "AxisPositions": null, "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Volts": "5.0000", "528-Name": "DA Shutter", "555-State": "1", "XLIGHT Dichroic-Label": "QUAD", "445 AO-Sequenceable": "1", "405-Name": "DA Shutter", "405 AO-IOChannel": "Dev1/ao0", "Core-Camera": "PRIME", "Focus-Name": "Focus", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "405 Shutter-IOChannel": "Dev1/ao8", "640 AO-Demo": "No", "640 Shutter-SupportsTriggering": "0", "445 AO-Demo": "No", "555-DA Device": "555 Shutter", "470 Shutter-Description": "NI DAC", "528 AO-Volts": "0.0000", "COM1-DataBits": "8", "Vincent-D3-Address": "x", "520 AO-SupportsTriggering": "0", "405-Description": "DA device that is used as a shutter", "640 Shutter-MinVolts": "0.0000", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "405 AO-Name": "AnalogIO", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "640 AO-TriggerInputLine": "/Dev1/PFI0", "405 AO-TriggerInputLine": "/Dev1/PFI0", "640-Description": "DA device that is used as a shutter", "445 Shutter-TriggerSampleRate": "100000", "470 Shutter-TriggerSequenceLength": "1000", "COM1-StopBits": "1", "520 Shutter-SupportsTriggering": "0", "405-State": "0", "Core-Initialize": "1", "XLIGHT Emission Wheel-State": "3", "520 AO-Description": "NI DAC", "528 AO-TriggerInputLine": "/Dev1/PFI0", "445 Shutter-MaxVolts": "5.0000", "COM9-Handshaking": "Off", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "XLIGHT Spin motor-Label": "On (spinning)", "PRIME-PP 5 ENABLED": "No", "UUID": "d8f724a4-b15d-4156-9da8-b90d316cc672", "PRIME-TriggerMode": "Internal Trigger", "COM1-Handshaking": "Off", "PRIME-Timing-PostTriggerDelayNs": "49344000.0000", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "Focus-Position": "17741.5000", "528 Shutter-Block voltage": "false", "Channel": "Il-T", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "555 Shutter-Demo": "No", "405 Shutter-Demo": "No", "528 AO-Name": "AnalogIO", "520-State": "0", "Core-Galvo": "", "405 Shutter-TriggerSampleRate": "100000", "Focus-FocusStepSize_(nm)": "100", "PRIME-PixelType": "16bit", "COM4-DataBits": "8", "470 AO-IOChannel": "Dev1/ao2", "520 AO-MinVolts": "0.0000", "COM9-Parity": "None", "PRIME-CircularBufferEnabled": "ON", "PRIME-PP 6 Reset": "No", "640 AO-Description": "NI DAC", "Source": "PRIME", "445 AO-MinVolts": "0.0000", "PIEZO-Name": "Stage", "445 AO-Description": "NI DAC", "PRIME-CircularBufferFrameCount": "46", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "520 AO-Volts": "0.0000", "528 Shutter-TriggerSampleRate": "100000", "XPositionUm": -1324.9, "OpticalPath-State": "4", "PRIME-PP 3 THRESHOLD": "80", "PRIME-PVCAM Version": "3.7.1", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Gain": "1-HDR", "640 Shutter-TriggerSequenceLength": "1000", "528 AO-SupportsTriggering": "0", "445 AO-TriggerInputLine": "/Dev1/PFI0", "OpticalPath-Name": "OpticalPath", "PRIME-PP 4 ENABLED": "Yes", "PRIME-PVCAM Adapter Version": "1.3.29", "COM3-Name": "COM3", "405 AO-MaxVolts": "5.0000", "520 Shutter-Block voltage": "false", "PRIME-Timing-ClearingTimeNs": "49344000.0000", "Core-Focus": "PIEZO", "528 AO-Block voltage": "false", "TE2000-Description": "Nikon TE2000 hub", "528 AO-MinVolts": "0.0000", "COM4-Parity": "None", "Summary": { "Slices": 11, "ChContrastMin": [ 0, 0, 0, 0, 0, 0 ], "PixelAspect": 1, "SlicesFirst": true, "Interval_ms": 0, "Time": "2019-12-19 09:59:21 -0500", "Prefix": "seq_13", "InitialPositionList": [ { "Label": "Pos0", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.21], "Focus": [17741.5], "XYStage": [ 44542.200000000004, 31636.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos1", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.22], "Focus": [17741.4], "XYStage": [ 43801.100000000006, 31637.9 ] }, "GridColumnIndex": 0 }, { "Label": "Pos2", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.5], "XYStage": [ 38561.3, 32040.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos3", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.185], "Focus": [17741.3], "XYStage": [ 37698.4, 32041.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos4", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.16], "Focus": [17741.5], "XYStage": [ 32375.4, 31758.9 ] }, "GridColumnIndex": 0 }, { "Label": "Pos5", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.17000000000002], "Focus": [17741.3], "XYStage": [ 31770.7, 31758.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos6", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.4], "XYStage": [ 26536.800000000003, 31068.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos7", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.205], "Focus": [17741.4], "XYStage": [ 25420.2, 31068.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos8", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.85], "Focus": [17741.3], "XYStage": [ 21059, 31508 ] }, "GridColumnIndex": 0 }, { "Label": "Pos9", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.82], "Focus": [17741.3], "XYStage": [ 19731.9, 31506.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos10", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.79], "Focus": [17741.4], "XYStage": [ 14528.5, 32007.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos11", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.83], "Focus": [17741.2], "XYStage": [ 13830.300000000001, 32009.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos12", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.79], "Focus": [17741.3], "XYStage": [ 8775.300000000001, 32017.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos13", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.815], "Focus": [17741.3], "XYStage": [ 8109.200000000001, 32008.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos14", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.805], "Focus": [17741.3], "XYStage": [ 3483.3, 31908.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos15", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.82], "Focus": [17741.2], "XYStage": [ 2686.8, 31903.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos16", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [211.48000000000002], "Focus": [17741.1], "XYStage": [ -1978.9, 31973.600000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos17", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [211.51500000000001], "Focus": [17741.1], "XYStage": [ -2957.8, 31982.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos18", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.195], "Focus": [17741.5], "XYStage": [ 47747.4, -576.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos19", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.23000000000002], "Focus": [17741.6], "XYStage": [ 47053.9, -576.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos20", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.205], "Focus": [17741.4], "XYStage": [ 41871.200000000004, -479.8 ] }, "GridColumnIndex": 0 }, { "Label": "Pos21", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.4], "XYStage": [ 40882.600000000006, -392 ] }, "GridColumnIndex": 0 }, { "Label": "Pos22", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.24], "Focus": [17741.4], "XYStage": [ 35905.6, -135.3 ] }, "GridColumnIndex": 0 }, { "Label": "Pos23", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.195], "Focus": [17741.5], "XYStage": [ 34990.9, -132.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos24", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.46], "Focus": [17741.4], "XYStage": [ 29678.100000000002, -958.1 ] }, "GridColumnIndex": 0 }, { "Label": "Pos25", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.44], "Focus": [17741.5], "XYStage": [ 28983.600000000002, -939.4000000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos26", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.475], "Focus": [17741.5], "XYStage": [ 23019.7, -122 ] }, "GridColumnIndex": 0 }, { "Label": "Pos27", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.44], "Focus": [17741.7], "XYStage": [ 22428, -116.80000000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos28", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.46], "Focus": [17741.7], "XYStage": [ 16364.900000000001, -181.10000000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos29", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.48000000000002], "Focus": [17741.5], "XYStage": [ 15712.1, -180 ] }, "GridColumnIndex": 0 }, { "Label": "Pos30", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.45000000000002], "Focus": [17741.7], "XYStage": [ 10346.1, 77.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos31", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.49], "Focus": [17741.4], "XYStage": [ 9755.5, 85.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos32", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.475], "Focus": [17741.5], "XYStage": [ 4775.5, 293.90000000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos33", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.445], "Focus": [17741.4], "XYStage": [ 3954.9, 293.90000000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos34", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.455], "Focus": [17741.4], "XYStage": [ -1324.9, 545.6 ] }, "GridColumnIndex": 0 }, { "Label": "Pos35", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.445], "Focus": [17741.3], "XYStage": [ -2097, 576.6 ] }, "GridColumnIndex": 0 } ], "z-step_um": 10, "GridRow": 0, "BitDepth": 16, "Depth": 2, "Channels": 6, "Source": "Micro-Manager", "ChColors": [ -16711681, -256, -65281, -1, -1, -16776961 ], "MetadataVersion": 10, "KeepShutterOpenChannels": true, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536, 65536 ], "CustomIntervals_ms": [], "Height": 1200, "MicroManagerVersion": "1.4.23 20180219", "GridColumn": 0, "UUID": "18808e7c-f2e6-47b3-90d8-1d779ce87dbd", "PixelType": "GRAY16", "Width": 1200, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\Yan\\20191218-YC113-S7S8-miseq", "Frames": 1, "Comment": "", "UserName": "Nikon", "PixelSize_um": 1.1, "ROI": [ 0, 0, 1200, 1200 ], "Date": "2019-12-19", "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC", "P-DAPI-high" ], "KeepShutterOpenSlices": true, "IJType": 1, "ComputerName": "ZadorConfocal2", "Positions": 36, "PositionIndex": 34, "CameraTimeout": 20000, "TimeFirst": false }, "640 AO-Name": "AnalogIO", "Vincent-D3-Command": "Close" }, "FrameKey-0-1-8": { "COM4-StopBits": "1", "528 AO-MaxVolts": "5.0000", "XLIGHT Excitation Wheel-State": "1", "470 Shutter-Name": "AnalogIO", "Core-Shutter": "555", "555 AO-Demo": "No", "XLIGHT Dichroic-State": "2", "XYStage-StepSize-Y": "0.1000", "ZPositionUm": 248.455, "XYStage-StepSize-X": "0.1000", "PRIME-FirmwareVersion": "20.28", "PRIME-ClearCycles": "2", "520-Name": "DA Shutter", "405 Shutter-Name": "AnalogIO", "PIEZO-StepSize": "0.0050", "PRIME-ClearMode": "Pre-Exposure", "470 AO-MinVolts": "0.0000", "405 AO-SupportsTriggering": "1", "CameraChannelIndex": 0, "XYStage-StartSpeed": "500.0000", "COM9-StopBits": "2", "405 AO-Description": "NI DAC", "COM4-DelayBetweenCharsMs": "0.0000", "445 AO-Block voltage": "false", "520 AO-TriggerInputLine": "/Dev1/PFI0", "555 AO-Description": "NI DAC", "520 AO-TriggerSampleRate": "100000", "COM4-Verbose": "1", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM4-Name": "COM4", "405 Shutter-Volts": "0.0000", "Vincent-D3-Port": "COM1", "PRIME-PP 4 MIN ADU AFFECTED": "200", "470 Shutter-MinVolts": "0.0000", "PRIME-PP 3 ENABLED": "Yes", "470 AO-Description": "NI DAC", "PRIME-Description": "PVCAM API device adapter", "520 Shutter-MaxVolts": "5.0000", "520 Shutter-Volts": "0.0000", "528 AO-Sequenceable": "1", "PRIME-Binning": "1x1", "555 Shutter-MaxVolts": "5.0000", "528 Shutter-TriggerSequenceLength": "1000", "405 Shutter-Block voltage": "false", "XLIGHT Spin motor-State": "1", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "Vincent-D3-Time to open (ms)": "35.0000", "Vincent-D3-Time to close (ms)": "35.0000", "445 AO-TriggerSampleRate": "100000", "640 Shutter-IOChannel": "Dev1/ao14", "555 AO-Sequenceable": "1", "520 AO-Name": "AnalogIO", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "470 AO-Block voltage": "false", "COM1-Verbose": "1", "COM9-AnswerTimeout": "10000.0000", "528 Shutter-Name": "AnalogIO", "PRIME-Trigger Timeout (secs)": "10", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "NextFrame": 0, "470 AO-TriggerSequenceLength": "1000", "PRIME-TransposeXY": "0", "445 AO-TriggerSequenceLength": "1000", "COM1-Name": "COM1", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "COM9-DataBits": "8", "COM1-AnswerTimeout": "500.0000", "Camera": "", "TE2000-Version": "V2.50-V2.50-V2.10", "528 Shutter-Volts": "0.0000", "XLIGHT Hub-Name": "XLIGHT Hub", "PIEZO-Description": "Ludl stage driver adapter", "PositionName": "Pos34", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSampleRate": "100000", "PRIME-FanSpeedSetpoint": "High", "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "405 AO-TriggerSampleRate": "100000", "470 Shutter-Block voltage": "false", "470-State": "0", "COM3-Parity": "None", "405 AO-Volts": "0.0000", "PRIME-TransposeMirrorY": "0", "PRIME-ChipName": "GS144BSI", "470 AO-Volts": "0.0000", "PRIME-TransposeMirrorX": "0", "445 Shutter-SupportsTriggering": "0", "470 Shutter-SupportsTriggering": "0", "XLIGHT Hub-Description": "XLIGHT hub", "PRIME-PreampOffLimit": "10000", "470 AO-Sequenceable": "1", "528 Shutter-MaxVolts": "5.0000", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "Core-XYStage": "XYStage", "470 AO-TriggerSampleRate": "100000", "PRIME-Timing-PreTriggerDelayNs": "24672000.0000", "528-DA Device": "528 Shutter", "Focus-Description": "Nikon TE2000 focus adapter", "FilterBlock-State": "5", "520 AO-Demo": "No", "COM3-DelayBetweenCharsMs": "0.0000", "470 Shutter-Volts": "0.0000", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "PRIME-PP 2 THRESHOLD": "125", "PRIME-PP 4 THRESHOLD": "75", "XYStage-Acceleration": "4.0000", "470 Shutter-Demo": "No", "PRIME-Name": "PMPCIECam00", "640-DA Device": "640 Shutter", "405 AO-Block voltage": "false", "ElapsedTime-ms": 1401380, "PRIME-Offset": "100", "445 AO-IOChannel": "Dev1/ao1", "640 AO-MaxVolts": "5.0000", "PositionIndex": 34, "PRIME-PP 1 ENABLED": "Yes", "COM1-BaudRate": "9600", "640-State": "0", "COM3-Verbose": "1", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "LudlController-TransmissionDelay": "0", "PRIME-FullWellCapacity": "62000", "PRIME-SMARTStreamingEnabled": "No", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "640 Shutter-Description": "NI DAC", "528-Description": "DA device that is used as a shutter", "COM4-Description": "Serial port driver (boost:asio)", "405 Shutter-Description": "NI DAC", "TE2000-Name": "TE2000", "528 Shutter-Description": "NI DAC", "COM9-Description": "Serial port driver (boost:asio)", "LudlController-Name": "LudlController", "445 Shutter-TriggerSequenceLength": "1000", "PRIME-SerialNumber": "A18B203008", "445 AO-Volts": "0.0000", "PRIME-X-dimension": "1200", "PRIME-ShutterCloseDelay": "0", "XLIGHT Disk slider-State": "2", "Position": "Default", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 AO-IOChannel": "Dev1/ao5", "520 AO-IOChannel": "Dev1/ao4", "528 AO-TriggerSequenceLength": "1000", "405 Shutter-MaxVolts": "5.0000", "470-DA Device": "470 Shutter", "PRIME-FTCapable": "No", "Core-ChannelGroup": "Channels", "555 AO-SupportsTriggering": "0", "PRIME-Y-dimension": "1200", "Frame": 0, "555 Shutter-Sequenceable": "1", "PRIME-Timing-ExposureTimeNs": "499998000.0000", "Core-ImageProcessor": "", "528-State": "0", "520 AO-Block voltage": "false", "XLIGHT Excitation Wheel-Label": "QUAD", "640 Shutter-Demo": "No", "COM3-StopBits": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "520 Shutter-IOChannel": "Dev1/ao11", "Width": 1200, "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-SupportsTriggering": "0", "640 AO-TriggerSampleRate": "100000", "520-Description": "DA device that is used as a shutter", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "405 Shutter-Sequenceable": "1", "405 Shutter-SupportsTriggering": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-TriggerSampleRate": "100000", "640 Shutter-MaxVolts": "5.0000", "555 AO-MaxVolts": "5.0000", "520 AO-TriggerSequenceLength": "1000", "COM3-BaudRate": "9600", "COM9-BaudRate": "9600", "Vincent-D3-Channel #": "Ch. #1", "PRIME-PMode": "Normal", "OpticalPath-Label": "Left(100)", "BitDepth": 16, "555 AO-TriggerSequenceLength": "1000", "445 Shutter-Description": "NI DAC", "405 AO-Sequenceable": "1", "PRIME-AcquisitionMethod": "Callbacks", "555 AO-Name": "AnalogIO", "PRIME-ShutterMode": "Pre-Exposure", "COM3-Handshaking": "Off", "445 AO-SupportsTriggering": "0", "ROI": "0-0-1200-1200", "COM9-Verbose": "1", "PRIME-Color": "OFF", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "PRIME-ShutterOpenDelay": "0", "COM1-Parity": "None", "Slice": 8, "SlicePosition": 30, "COM9-DelayBetweenCharsMs": "0.0000", "555-Name": "DA Shutter", "PIEZO-LudlSingleAxisName": "B", "XLIGHT Emission Wheel-Label": "585/11(T)", "640 Shutter-Name": "AnalogIO", "640 AO-Sequenceable": "1", "520 Shutter-Demo": "No", "528 Shutter-Demo": "No", "470-Name": "DA Shutter", "520-DA Device": "520 Shutter", "XYStage-Description": "Ludl XY stage driver adapter", "445 Shutter-MinVolts": "0.0000", "528 AO-TriggerSampleRate": "100000", "405 AO-MinVolts": "0.0000", "405 Shutter-TriggerSequenceLength": "1000", "Height": 1200, "555 AO-Block voltage": "false", "640 Shutter-Volts": "0.0000", "PixelType": "GRAY16", "445 Shutter-Block voltage": "false", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "520 Shutter-TriggerSequenceLength": "1000", "COM9-Name": "COM9", "Core-SLM": "", "555 AO-Volts": "4.5000", "555 Shutter-Block voltage": "false", "XYStage-Speed": "1800.0000", "520 AO-MaxVolts": "5.0000", "528 Shutter-SupportsTriggering": "0", "COM1-Description": "Serial port driver (boost:asio)", "PRIME-MetadataEnabled": "No", "520 Shutter-MinVolts": "0.0000", "470 Shutter-Sequenceable": "1", "PRIME-PreampDelay": "0", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-MinVolts": "0.0000", "ChannelIndex": 1, "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Core-AutoShutter": "1", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "Exposure-ms": 160, "PRIME-PP 2 ENABLED": "Yes", "640 Shutter-TriggerSampleRate": "100000", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "470 Shutter-IOChannel": "Dev1/ao10", "405 AO-Demo": "No", "PixelSizeUm": 1.1, "470 AO-Demo": "No", "470 Shutter-MaxVolts": "5.0000", "Vincent-D3-Name": "Vincent-D3", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FileName": "img_000000000_Il-T_008.tif", "LudlController-Reset": "Operate", "PRIME-Exposure": "160.00", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "445-Name": "DA Shutter", "555 Shutter-Name": "AnalogIO", "520 Shutter-TriggerSampleRate": "100000", "445 Shutter-IOChannel": "Dev1/ao9", "PRIME-TransposeCorrection": "0", "XYStage-StepSize": "0.1000", "445-Description": "DA device that is used as a shutter", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 AO-IOChannel": "Dev1/ao7", "520 Shutter-Name": "AnalogIO", "COM3-DataBits": "8", "640 AO-Volts": "0.0000", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "555 Shutter-IOChannel": "Dev1/ao13", "WaitInterval": null, "520 Shutter-Sequenceable": "1", "YPositionUm": 545.6, "445-State": "0", "445-DA Device": "445 Shutter", "PRIME-CircularBufferAutoSize": "ON", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "555 AO-TriggerSampleRate": "100000", "445 Shutter-Volts": "0.0000", "XLIGHT Hub-Port": "COM3", "Core-AutoFocus": "", "COM4-Handshaking": "Off", "528 Shutter-MinVolts": "0.0000", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "445 Shutter-Name": "AnalogIO", "528 Shutter-Sequenceable": "1", "XYStage-TransposeMirrorX": "0", "XYStage-TransposeMirrorY": "0", "PRIME-ReadoutRate": "100MHz 16bit", "XYStage-Name": "Stage", "640 AO-SupportsTriggering": "0", "640-Name": "DA Shutter", "640 Shutter-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "640 AO-Block voltage": "false", "445 Shutter-Sequenceable": "1", "470 AO-MaxVolts": "5.0000", "COM4-BaudRate": "9600", "470-Description": "DA device that is used as a shutter", "520 Shutter-Description": "NI DAC", "PRIME-CCDTemperature": "-14.8500", "Time": "2019-12-19 09:59:31 -0500", "640 AO-TriggerSequenceLength": "1000", "470 AO-Name": "AnalogIO", "XLIGHT Disk slider-Label": "Disk Pos 40um", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "Binning": "1x1", "TE2000-ModelType": "1", "COM3-AnswerTimeout": "500.0000", "555 Shutter-SupportsTriggering": "0", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "TE2000-Port": "COM4", "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Description": "NI DAC", "COM4-AnswerTimeout": "500.0000", "COM1-DelayBetweenCharsMs": "0.0000", "FilterBlock-Label": "Confocal", "445 AO-Name": "AnalogIO", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-Timing-ReadoutTimeNs": "24672000.0000", "528 AO-Demo": "No", "520 AO-Sequenceable": "1", "555-Description": "DA device that is used as a shutter", "405-DA Device": "405 Shutter", "405 AO-TriggerSequenceLength": "1000", "640 AO-MinVolts": "0.0000", "640 Shutter-Block voltage": "false", "FrameIndex": 0, "PRIME-Port": "CMOS", "555 AO-MinVolts": "0.0000", "COM3-Description": "Serial port driver (boost:asio)", "528 AO-Description": "NI DAC", "555 AO-IOChannel": "Dev1/ao6", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "555 AO-TriggerInputLine": "/Dev1/PFI0", "PRIME-ActualInterval-ms": "390.5200", "LudlController-Port": "COM9", "445 Shutter-Demo": "No", "PRIME-Trigger-Expose Out-Mux": "1", "SliceIndex": 8, "PRIME-CCDTemperatureSetPoint": "-15.0000", "405 Shutter-MinVolts": "0.0000", "PRIME-ExposeOutMode": "First Row", "AxisPositions": null, "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Volts": "5.0000", "528-Name": "DA Shutter", "555-State": "1", "XLIGHT Dichroic-Label": "QUAD", "445 AO-Sequenceable": "1", "405-Name": "DA Shutter", "405 AO-IOChannel": "Dev1/ao0", "Core-Camera": "PRIME", "Focus-Name": "Focus", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "405 Shutter-IOChannel": "Dev1/ao8", "640 AO-Demo": "No", "640 Shutter-SupportsTriggering": "0", "445 AO-Demo": "No", "555-DA Device": "555 Shutter", "470 Shutter-Description": "NI DAC", "528 AO-Volts": "0.0000", "COM1-DataBits": "8", "Vincent-D3-Address": "x", "520 AO-SupportsTriggering": "0", "405-Description": "DA device that is used as a shutter", "640 Shutter-MinVolts": "0.0000", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "405 AO-Name": "AnalogIO", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "640 AO-TriggerInputLine": "/Dev1/PFI0", "405 AO-TriggerInputLine": "/Dev1/PFI0", "640-Description": "DA device that is used as a shutter", "445 Shutter-TriggerSampleRate": "100000", "470 Shutter-TriggerSequenceLength": "1000", "COM1-StopBits": "1", "520 Shutter-SupportsTriggering": "0", "405-State": "0", "Core-Initialize": "1", "XLIGHT Emission Wheel-State": "3", "520 AO-Description": "NI DAC", "528 AO-TriggerInputLine": "/Dev1/PFI0", "445 Shutter-MaxVolts": "5.0000", "COM9-Handshaking": "Off", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "XLIGHT Spin motor-Label": "On (spinning)", "PRIME-PP 5 ENABLED": "No", "UUID": "12f200ef-e55c-48cd-8098-99e5e2caf5ff", "PRIME-TriggerMode": "Internal Trigger", "COM1-Handshaking": "Off", "PRIME-Timing-PostTriggerDelayNs": "49344000.0000", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "Focus-Position": "17741.5000", "528 Shutter-Block voltage": "false", "Channel": "Il-T", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "555 Shutter-Demo": "No", "405 Shutter-Demo": "No", "528 AO-Name": "AnalogIO", "520-State": "0", "Core-Galvo": "", "405 Shutter-TriggerSampleRate": "100000", "Focus-FocusStepSize_(nm)": "100", "PRIME-PixelType": "16bit", "COM4-DataBits": "8", "470 AO-IOChannel": "Dev1/ao2", "520 AO-MinVolts": "0.0000", "COM9-Parity": "None", "PRIME-CircularBufferEnabled": "ON", "PRIME-PP 6 Reset": "No", "640 AO-Description": "NI DAC", "Source": "PRIME", "445 AO-MinVolts": "0.0000", "PIEZO-Name": "Stage", "445 AO-Description": "NI DAC", "PRIME-CircularBufferFrameCount": "46", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "520 AO-Volts": "0.0000", "528 Shutter-TriggerSampleRate": "100000", "XPositionUm": -1324.9, "OpticalPath-State": "4", "PRIME-PP 3 THRESHOLD": "80", "PRIME-PVCAM Version": "3.7.1", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Gain": "1-HDR", "640 Shutter-TriggerSequenceLength": "1000", "528 AO-SupportsTriggering": "0", "445 AO-TriggerInputLine": "/Dev1/PFI0", "OpticalPath-Name": "OpticalPath", "PRIME-PP 4 ENABLED": "Yes", "PRIME-PVCAM Adapter Version": "1.3.29", "COM3-Name": "COM3", "405 AO-MaxVolts": "5.0000", "520 Shutter-Block voltage": "false", "PRIME-Timing-ClearingTimeNs": "49344000.0000", "Core-Focus": "PIEZO", "528 AO-Block voltage": "false", "TE2000-Description": "Nikon TE2000 hub", "528 AO-MinVolts": "0.0000", "COM4-Parity": "None", "Summary": { "Slices": 11, "ChContrastMin": [ 0, 0, 0, 0, 0, 0 ], "PixelAspect": 1, "SlicesFirst": true, "Interval_ms": 0, "Time": "2019-12-19 09:59:21 -0500", "Prefix": "seq_13", "InitialPositionList": [ { "Label": "Pos0", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.21], "Focus": [17741.5], "XYStage": [ 44542.200000000004, 31636.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos1", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.22], "Focus": [17741.4], "XYStage": [ 43801.100000000006, 31637.9 ] }, "GridColumnIndex": 0 }, { "Label": "Pos2", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.5], "XYStage": [ 38561.3, 32040.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos3", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.185], "Focus": [17741.3], "XYStage": [ 37698.4, 32041.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos4", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.16], "Focus": [17741.5], "XYStage": [ 32375.4, 31758.9 ] }, "GridColumnIndex": 0 }, { "Label": "Pos5", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.17000000000002], "Focus": [17741.3], "XYStage": [ 31770.7, 31758.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos6", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.4], "XYStage": [ 26536.800000000003, 31068.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos7", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.205], "Focus": [17741.4], "XYStage": [ 25420.2, 31068.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos8", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.85], "Focus": [17741.3], "XYStage": [ 21059, 31508 ] }, "GridColumnIndex": 0 }, { "Label": "Pos9", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.82], "Focus": [17741.3], "XYStage": [ 19731.9, 31506.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos10", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.79], "Focus": [17741.4], "XYStage": [ 14528.5, 32007.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos11", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.83], "Focus": [17741.2], "XYStage": [ 13830.300000000001, 32009.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos12", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.79], "Focus": [17741.3], "XYStage": [ 8775.300000000001, 32017.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos13", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.815], "Focus": [17741.3], "XYStage": [ 8109.200000000001, 32008.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos14", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.805], "Focus": [17741.3], "XYStage": [ 3483.3, 31908.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos15", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.82], "Focus": [17741.2], "XYStage": [ 2686.8, 31903.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos16", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [211.48000000000002], "Focus": [17741.1], "XYStage": [ -1978.9, 31973.600000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos17", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [211.51500000000001], "Focus": [17741.1], "XYStage": [ -2957.8, 31982.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos18", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.195], "Focus": [17741.5], "XYStage": [ 47747.4, -576.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos19", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.23000000000002], "Focus": [17741.6], "XYStage": [ 47053.9, -576.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos20", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.205], "Focus": [17741.4], "XYStage": [ 41871.200000000004, -479.8 ] }, "GridColumnIndex": 0 }, { "Label": "Pos21", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.4], "XYStage": [ 40882.600000000006, -392 ] }, "GridColumnIndex": 0 }, { "Label": "Pos22", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.24], "Focus": [17741.4], "XYStage": [ 35905.6, -135.3 ] }, "GridColumnIndex": 0 }, { "Label": "Pos23", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.195], "Focus": [17741.5], "XYStage": [ 34990.9, -132.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos24", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.46], "Focus": [17741.4], "XYStage": [ 29678.100000000002, -958.1 ] }, "GridColumnIndex": 0 }, { "Label": "Pos25", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.44], "Focus": [17741.5], "XYStage": [ 28983.600000000002, -939.4000000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos26", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.475], "Focus": [17741.5], "XYStage": [ 23019.7, -122 ] }, "GridColumnIndex": 0 }, { "Label": "Pos27", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.44], "Focus": [17741.7], "XYStage": [ 22428, -116.80000000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos28", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.46], "Focus": [17741.7], "XYStage": [ 16364.900000000001, -181.10000000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos29", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.48000000000002], "Focus": [17741.5], "XYStage": [ 15712.1, -180 ] }, "GridColumnIndex": 0 }, { "Label": "Pos30", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.45000000000002], "Focus": [17741.7], "XYStage": [ 10346.1, 77.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos31", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.49], "Focus": [17741.4], "XYStage": [ 9755.5, 85.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos32", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.475], "Focus": [17741.5], "XYStage": [ 4775.5, 293.90000000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos33", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.445], "Focus": [17741.4], "XYStage": [ 3954.9, 293.90000000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos34", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.455], "Focus": [17741.4], "XYStage": [ -1324.9, 545.6 ] }, "GridColumnIndex": 0 }, { "Label": "Pos35", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.445], "Focus": [17741.3], "XYStage": [ -2097, 576.6 ] }, "GridColumnIndex": 0 } ], "z-step_um": 10, "GridRow": 0, "BitDepth": 16, "Depth": 2, "Channels": 6, "Source": "Micro-Manager", "ChColors": [ -16711681, -256, -65281, -1, -1, -16776961 ], "MetadataVersion": 10, "KeepShutterOpenChannels": true, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536, 65536 ], "CustomIntervals_ms": [], "Height": 1200, "MicroManagerVersion": "1.4.23 20180219", "GridColumn": 0, "UUID": "18808e7c-f2e6-47b3-90d8-1d779ce87dbd", "PixelType": "GRAY16", "Width": 1200, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\Yan\\20191218-YC113-S7S8-miseq", "Frames": 1, "Comment": "", "UserName": "Nikon", "PixelSize_um": 1.1, "ROI": [ 0, 0, 1200, 1200 ], "Date": "2019-12-19", "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC", "P-DAPI-high" ], "KeepShutterOpenSlices": true, "IJType": 1, "ComputerName": "ZadorConfocal2", "Positions": 36, "PositionIndex": 34, "CameraTimeout": 20000, "TimeFirst": false }, "640 AO-Name": "AnalogIO", "Vincent-D3-Command": "Close" }, "FrameKey-0-1-9": { "COM4-StopBits": "1", "528 AO-MaxVolts": "5.0000", "XLIGHT Excitation Wheel-State": "1", "470 Shutter-Name": "AnalogIO", "Core-Shutter": "555", "555 AO-Demo": "No", "XLIGHT Dichroic-State": "2", "XYStage-StepSize-Y": "0.1000", "ZPositionUm": 258.45500000000004, "XYStage-StepSize-X": "0.1000", "PRIME-FirmwareVersion": "20.28", "PRIME-ClearCycles": "2", "520-Name": "DA Shutter", "405 Shutter-Name": "AnalogIO", "PIEZO-StepSize": "0.0050", "PRIME-ClearMode": "Pre-Exposure", "470 AO-MinVolts": "0.0000", "405 AO-SupportsTriggering": "1", "CameraChannelIndex": 0, "XYStage-StartSpeed": "500.0000", "COM9-StopBits": "2", "405 AO-Description": "NI DAC", "COM4-DelayBetweenCharsMs": "0.0000", "445 AO-Block voltage": "false", "520 AO-TriggerInputLine": "/Dev1/PFI0", "555 AO-Description": "NI DAC", "520 AO-TriggerSampleRate": "100000", "COM4-Verbose": "1", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM4-Name": "COM4", "405 Shutter-Volts": "0.0000", "Vincent-D3-Port": "COM1", "PRIME-PP 4 MIN ADU AFFECTED": "200", "470 Shutter-MinVolts": "0.0000", "PRIME-PP 3 ENABLED": "Yes", "470 AO-Description": "NI DAC", "PRIME-Description": "PVCAM API device adapter", "520 Shutter-MaxVolts": "5.0000", "520 Shutter-Volts": "0.0000", "528 AO-Sequenceable": "1", "PRIME-Binning": "1x1", "555 Shutter-MaxVolts": "5.0000", "528 Shutter-TriggerSequenceLength": "1000", "405 Shutter-Block voltage": "false", "XLIGHT Spin motor-State": "1", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "Vincent-D3-Time to open (ms)": "35.0000", "Vincent-D3-Time to close (ms)": "35.0000", "445 AO-TriggerSampleRate": "100000", "640 Shutter-IOChannel": "Dev1/ao14", "555 AO-Sequenceable": "1", "520 AO-Name": "AnalogIO", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "470 AO-Block voltage": "false", "COM1-Verbose": "1", "COM9-AnswerTimeout": "10000.0000", "528 Shutter-Name": "AnalogIO", "PRIME-Trigger Timeout (secs)": "10", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "NextFrame": 0, "470 AO-TriggerSequenceLength": "1000", "PRIME-TransposeXY": "0", "445 AO-TriggerSequenceLength": "1000", "COM1-Name": "COM1", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "COM9-DataBits": "8", "COM1-AnswerTimeout": "500.0000", "Camera": "", "TE2000-Version": "V2.50-V2.50-V2.10", "528 Shutter-Volts": "0.0000", "XLIGHT Hub-Name": "XLIGHT Hub", "PIEZO-Description": "Ludl stage driver adapter", "PositionName": "Pos34", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSampleRate": "100000", "PRIME-FanSpeedSetpoint": "High", "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "405 AO-TriggerSampleRate": "100000", "470 Shutter-Block voltage": "false", "470-State": "0", "COM3-Parity": "None", "405 AO-Volts": "0.0000", "PRIME-TransposeMirrorY": "0", "PRIME-ChipName": "GS144BSI", "470 AO-Volts": "0.0000", "PRIME-TransposeMirrorX": "0", "445 Shutter-SupportsTriggering": "0", "470 Shutter-SupportsTriggering": "0", "XLIGHT Hub-Description": "XLIGHT hub", "PRIME-PreampOffLimit": "10000", "470 AO-Sequenceable": "1", "528 Shutter-MaxVolts": "5.0000", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "Core-XYStage": "XYStage", "470 AO-TriggerSampleRate": "100000", "PRIME-Timing-PreTriggerDelayNs": "24672000.0000", "528-DA Device": "528 Shutter", "Focus-Description": "Nikon TE2000 focus adapter", "FilterBlock-State": "5", "520 AO-Demo": "No", "COM3-DelayBetweenCharsMs": "0.0000", "470 Shutter-Volts": "0.0000", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "PRIME-PP 2 THRESHOLD": "125", "PRIME-PP 4 THRESHOLD": "75", "XYStage-Acceleration": "4.0000", "470 Shutter-Demo": "No", "PRIME-Name": "PMPCIECam00", "640-DA Device": "640 Shutter", "405 AO-Block voltage": "false", "ElapsedTime-ms": 1401833, "PRIME-Offset": "100", "445 AO-IOChannel": "Dev1/ao1", "640 AO-MaxVolts": "5.0000", "PositionIndex": 34, "PRIME-PP 1 ENABLED": "Yes", "COM1-BaudRate": "9600", "640-State": "0", "COM3-Verbose": "1", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "LudlController-TransmissionDelay": "0", "PRIME-FullWellCapacity": "62000", "PRIME-SMARTStreamingEnabled": "No", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "640 Shutter-Description": "NI DAC", "528-Description": "DA device that is used as a shutter", "COM4-Description": "Serial port driver (boost:asio)", "405 Shutter-Description": "NI DAC", "TE2000-Name": "TE2000", "528 Shutter-Description": "NI DAC", "COM9-Description": "Serial port driver (boost:asio)", "LudlController-Name": "LudlController", "445 Shutter-TriggerSequenceLength": "1000", "PRIME-SerialNumber": "A18B203008", "445 AO-Volts": "0.0000", "PRIME-X-dimension": "1200", "PRIME-ShutterCloseDelay": "0", "XLIGHT Disk slider-State": "2", "Position": "Default", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 AO-IOChannel": "Dev1/ao5", "520 AO-IOChannel": "Dev1/ao4", "528 AO-TriggerSequenceLength": "1000", "405 Shutter-MaxVolts": "5.0000", "470-DA Device": "470 Shutter", "PRIME-FTCapable": "No", "Core-ChannelGroup": "Channels", "555 AO-SupportsTriggering": "0", "PRIME-Y-dimension": "1200", "Frame": 0, "555 Shutter-Sequenceable": "1", "PRIME-Timing-ExposureTimeNs": "499998000.0000", "Core-ImageProcessor": "", "528-State": "0", "520 AO-Block voltage": "false", "XLIGHT Excitation Wheel-Label": "QUAD", "640 Shutter-Demo": "No", "COM3-StopBits": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "520 Shutter-IOChannel": "Dev1/ao11", "Width": 1200, "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-SupportsTriggering": "0", "640 AO-TriggerSampleRate": "100000", "520-Description": "DA device that is used as a shutter", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "405 Shutter-Sequenceable": "1", "405 Shutter-SupportsTriggering": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-TriggerSampleRate": "100000", "640 Shutter-MaxVolts": "5.0000", "555 AO-MaxVolts": "5.0000", "520 AO-TriggerSequenceLength": "1000", "COM3-BaudRate": "9600", "COM9-BaudRate": "9600", "Vincent-D3-Channel #": "Ch. #1", "PRIME-PMode": "Normal", "OpticalPath-Label": "Left(100)", "BitDepth": 16, "555 AO-TriggerSequenceLength": "1000", "445 Shutter-Description": "NI DAC", "405 AO-Sequenceable": "1", "PRIME-AcquisitionMethod": "Callbacks", "555 AO-Name": "AnalogIO", "PRIME-ShutterMode": "Pre-Exposure", "COM3-Handshaking": "Off", "445 AO-SupportsTriggering": "0", "ROI": "0-0-1200-1200", "COM9-Verbose": "1", "PRIME-Color": "OFF", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "PRIME-ShutterOpenDelay": "0", "COM1-Parity": "None", "Slice": 9, "SlicePosition": 40, "COM9-DelayBetweenCharsMs": "0.0000", "555-Name": "DA Shutter", "PIEZO-LudlSingleAxisName": "B", "XLIGHT Emission Wheel-Label": "585/11(T)", "640 Shutter-Name": "AnalogIO", "640 AO-Sequenceable": "1", "520 Shutter-Demo": "No", "528 Shutter-Demo": "No", "470-Name": "DA Shutter", "520-DA Device": "520 Shutter", "XYStage-Description": "Ludl XY stage driver adapter", "445 Shutter-MinVolts": "0.0000", "528 AO-TriggerSampleRate": "100000", "405 AO-MinVolts": "0.0000", "405 Shutter-TriggerSequenceLength": "1000", "Height": 1200, "555 AO-Block voltage": "false", "640 Shutter-Volts": "0.0000", "PixelType": "GRAY16", "445 Shutter-Block voltage": "false", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "520 Shutter-TriggerSequenceLength": "1000", "COM9-Name": "COM9", "Core-SLM": "", "555 AO-Volts": "4.5000", "555 Shutter-Block voltage": "false", "XYStage-Speed": "1800.0000", "520 AO-MaxVolts": "5.0000", "528 Shutter-SupportsTriggering": "0", "COM1-Description": "Serial port driver (boost:asio)", "PRIME-MetadataEnabled": "No", "520 Shutter-MinVolts": "0.0000", "470 Shutter-Sequenceable": "1", "PRIME-PreampDelay": "0", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-MinVolts": "0.0000", "ChannelIndex": 1, "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Core-AutoShutter": "1", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "Exposure-ms": 160, "PRIME-PP 2 ENABLED": "Yes", "640 Shutter-TriggerSampleRate": "100000", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "470 Shutter-IOChannel": "Dev1/ao10", "405 AO-Demo": "No", "PixelSizeUm": 1.1, "470 AO-Demo": "No", "470 Shutter-MaxVolts": "5.0000", "Vincent-D3-Name": "Vincent-D3", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FileName": "img_000000000_Il-T_009.tif", "LudlController-Reset": "Operate", "PRIME-Exposure": "160.00", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "445-Name": "DA Shutter", "555 Shutter-Name": "AnalogIO", "520 Shutter-TriggerSampleRate": "100000", "445 Shutter-IOChannel": "Dev1/ao9", "PRIME-TransposeCorrection": "0", "XYStage-StepSize": "0.1000", "445-Description": "DA device that is used as a shutter", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 AO-IOChannel": "Dev1/ao7", "520 Shutter-Name": "AnalogIO", "COM3-DataBits": "8", "640 AO-Volts": "0.0000", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "555 Shutter-IOChannel": "Dev1/ao13", "WaitInterval": null, "520 Shutter-Sequenceable": "1", "YPositionUm": 545.6, "445-State": "0", "445-DA Device": "445 Shutter", "PRIME-CircularBufferAutoSize": "ON", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "555 AO-TriggerSampleRate": "100000", "445 Shutter-Volts": "0.0000", "XLIGHT Hub-Port": "COM3", "Core-AutoFocus": "", "COM4-Handshaking": "Off", "528 Shutter-MinVolts": "0.0000", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "445 Shutter-Name": "AnalogIO", "528 Shutter-Sequenceable": "1", "XYStage-TransposeMirrorX": "0", "XYStage-TransposeMirrorY": "0", "PRIME-ReadoutRate": "100MHz 16bit", "XYStage-Name": "Stage", "640 AO-SupportsTriggering": "0", "640-Name": "DA Shutter", "640 Shutter-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "640 AO-Block voltage": "false", "445 Shutter-Sequenceable": "1", "470 AO-MaxVolts": "5.0000", "COM4-BaudRate": "9600", "470-Description": "DA device that is used as a shutter", "520 Shutter-Description": "NI DAC", "PRIME-CCDTemperature": "-14.8500", "Time": "2019-12-19 09:59:32 -0500", "640 AO-TriggerSequenceLength": "1000", "470 AO-Name": "AnalogIO", "XLIGHT Disk slider-Label": "Disk Pos 40um", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "Binning": "1x1", "TE2000-ModelType": "1", "COM3-AnswerTimeout": "500.0000", "555 Shutter-SupportsTriggering": "0", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "TE2000-Port": "COM4", "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Description": "NI DAC", "COM4-AnswerTimeout": "500.0000", "COM1-DelayBetweenCharsMs": "0.0000", "FilterBlock-Label": "Confocal", "445 AO-Name": "AnalogIO", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-Timing-ReadoutTimeNs": "24672000.0000", "528 AO-Demo": "No", "520 AO-Sequenceable": "1", "555-Description": "DA device that is used as a shutter", "405-DA Device": "405 Shutter", "405 AO-TriggerSequenceLength": "1000", "640 AO-MinVolts": "0.0000", "640 Shutter-Block voltage": "false", "FrameIndex": 0, "PRIME-Port": "CMOS", "555 AO-MinVolts": "0.0000", "COM3-Description": "Serial port driver (boost:asio)", "528 AO-Description": "NI DAC", "555 AO-IOChannel": "Dev1/ao6", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "555 AO-TriggerInputLine": "/Dev1/PFI0", "PRIME-ActualInterval-ms": "390.5200", "LudlController-Port": "COM9", "445 Shutter-Demo": "No", "PRIME-Trigger-Expose Out-Mux": "1", "SliceIndex": 9, "PRIME-CCDTemperatureSetPoint": "-15.0000", "405 Shutter-MinVolts": "0.0000", "PRIME-ExposeOutMode": "First Row", "AxisPositions": null, "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Volts": "5.0000", "528-Name": "DA Shutter", "555-State": "1", "XLIGHT Dichroic-Label": "QUAD", "445 AO-Sequenceable": "1", "405-Name": "DA Shutter", "405 AO-IOChannel": "Dev1/ao0", "Core-Camera": "PRIME", "Focus-Name": "Focus", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "405 Shutter-IOChannel": "Dev1/ao8", "640 AO-Demo": "No", "640 Shutter-SupportsTriggering": "0", "445 AO-Demo": "No", "555-DA Device": "555 Shutter", "470 Shutter-Description": "NI DAC", "528 AO-Volts": "0.0000", "COM1-DataBits": "8", "Vincent-D3-Address": "x", "520 AO-SupportsTriggering": "0", "405-Description": "DA device that is used as a shutter", "640 Shutter-MinVolts": "0.0000", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "405 AO-Name": "AnalogIO", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "640 AO-TriggerInputLine": "/Dev1/PFI0", "405 AO-TriggerInputLine": "/Dev1/PFI0", "640-Description": "DA device that is used as a shutter", "445 Shutter-TriggerSampleRate": "100000", "470 Shutter-TriggerSequenceLength": "1000", "COM1-StopBits": "1", "520 Shutter-SupportsTriggering": "0", "405-State": "0", "Core-Initialize": "1", "XLIGHT Emission Wheel-State": "3", "520 AO-Description": "NI DAC", "528 AO-TriggerInputLine": "/Dev1/PFI0", "445 Shutter-MaxVolts": "5.0000", "COM9-Handshaking": "Off", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "XLIGHT Spin motor-Label": "On (spinning)", "PRIME-PP 5 ENABLED": "No", "UUID": "ce580697-114c-49d6-ac6e-d60b81520aeb", "PRIME-TriggerMode": "Internal Trigger", "COM1-Handshaking": "Off", "PRIME-Timing-PostTriggerDelayNs": "49344000.0000", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "Focus-Position": "17741.5000", "528 Shutter-Block voltage": "false", "Channel": "Il-T", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "555 Shutter-Demo": "No", "405 Shutter-Demo": "No", "528 AO-Name": "AnalogIO", "520-State": "0", "Core-Galvo": "", "405 Shutter-TriggerSampleRate": "100000", "Focus-FocusStepSize_(nm)": "100", "PRIME-PixelType": "16bit", "COM4-DataBits": "8", "470 AO-IOChannel": "Dev1/ao2", "520 AO-MinVolts": "0.0000", "COM9-Parity": "None", "PRIME-CircularBufferEnabled": "ON", "PRIME-PP 6 Reset": "No", "640 AO-Description": "NI DAC", "Source": "PRIME", "445 AO-MinVolts": "0.0000", "PIEZO-Name": "Stage", "445 AO-Description": "NI DAC", "PRIME-CircularBufferFrameCount": "46", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "520 AO-Volts": "0.0000", "528 Shutter-TriggerSampleRate": "100000", "XPositionUm": -1324.9, "OpticalPath-State": "4", "PRIME-PP 3 THRESHOLD": "80", "PRIME-PVCAM Version": "3.7.1", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Gain": "1-HDR", "640 Shutter-TriggerSequenceLength": "1000", "528 AO-SupportsTriggering": "0", "445 AO-TriggerInputLine": "/Dev1/PFI0", "OpticalPath-Name": "OpticalPath", "PRIME-PP 4 ENABLED": "Yes", "PRIME-PVCAM Adapter Version": "1.3.29", "COM3-Name": "COM3", "405 AO-MaxVolts": "5.0000", "520 Shutter-Block voltage": "false", "PRIME-Timing-ClearingTimeNs": "49344000.0000", "Core-Focus": "PIEZO", "528 AO-Block voltage": "false", "TE2000-Description": "Nikon TE2000 hub", "528 AO-MinVolts": "0.0000", "COM4-Parity": "None", "Summary": { "Slices": 11, "ChContrastMin": [ 0, 0, 0, 0, 0, 0 ], "PixelAspect": 1, "SlicesFirst": true, "Interval_ms": 0, "Time": "2019-12-19 09:59:21 -0500", "Prefix": "seq_13", "InitialPositionList": [ { "Label": "Pos0", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.21], "Focus": [17741.5], "XYStage": [ 44542.200000000004, 31636.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos1", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.22], "Focus": [17741.4], "XYStage": [ 43801.100000000006, 31637.9 ] }, "GridColumnIndex": 0 }, { "Label": "Pos2", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.5], "XYStage": [ 38561.3, 32040.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos3", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.185], "Focus": [17741.3], "XYStage": [ 37698.4, 32041.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos4", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.16], "Focus": [17741.5], "XYStage": [ 32375.4, 31758.9 ] }, "GridColumnIndex": 0 }, { "Label": "Pos5", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.17000000000002], "Focus": [17741.3], "XYStage": [ 31770.7, 31758.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos6", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.4], "XYStage": [ 26536.800000000003, 31068.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos7", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.205], "Focus": [17741.4], "XYStage": [ 25420.2, 31068.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos8", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.85], "Focus": [17741.3], "XYStage": [ 21059, 31508 ] }, "GridColumnIndex": 0 }, { "Label": "Pos9", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.82], "Focus": [17741.3], "XYStage": [ 19731.9, 31506.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos10", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.79], "Focus": [17741.4], "XYStage": [ 14528.5, 32007.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos11", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.83], "Focus": [17741.2], "XYStage": [ 13830.300000000001, 32009.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos12", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.79], "Focus": [17741.3], "XYStage": [ 8775.300000000001, 32017.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos13", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.815], "Focus": [17741.3], "XYStage": [ 8109.200000000001, 32008.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos14", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.805], "Focus": [17741.3], "XYStage": [ 3483.3, 31908.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos15", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.82], "Focus": [17741.2], "XYStage": [ 2686.8, 31903.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos16", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [211.48000000000002], "Focus": [17741.1], "XYStage": [ -1978.9, 31973.600000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos17", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [211.51500000000001], "Focus": [17741.1], "XYStage": [ -2957.8, 31982.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos18", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.195], "Focus": [17741.5], "XYStage": [ 47747.4, -576.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos19", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.23000000000002], "Focus": [17741.6], "XYStage": [ 47053.9, -576.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos20", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.205], "Focus": [17741.4], "XYStage": [ 41871.200000000004, -479.8 ] }, "GridColumnIndex": 0 }, { "Label": "Pos21", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.4], "XYStage": [ 40882.600000000006, -392 ] }, "GridColumnIndex": 0 }, { "Label": "Pos22", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.24], "Focus": [17741.4], "XYStage": [ 35905.6, -135.3 ] }, "GridColumnIndex": 0 }, { "Label": "Pos23", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.195], "Focus": [17741.5], "XYStage": [ 34990.9, -132.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos24", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.46], "Focus": [17741.4], "XYStage": [ 29678.100000000002, -958.1 ] }, "GridColumnIndex": 0 }, { "Label": "Pos25", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.44], "Focus": [17741.5], "XYStage": [ 28983.600000000002, -939.4000000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos26", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.475], "Focus": [17741.5], "XYStage": [ 23019.7, -122 ] }, "GridColumnIndex": 0 }, { "Label": "Pos27", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.44], "Focus": [17741.7], "XYStage": [ 22428, -116.80000000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos28", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.46], "Focus": [17741.7], "XYStage": [ 16364.900000000001, -181.10000000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos29", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.48000000000002], "Focus": [17741.5], "XYStage": [ 15712.1, -180 ] }, "GridColumnIndex": 0 }, { "Label": "Pos30", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.45000000000002], "Focus": [17741.7], "XYStage": [ 10346.1, 77.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos31", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.49], "Focus": [17741.4], "XYStage": [ 9755.5, 85.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos32", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.475], "Focus": [17741.5], "XYStage": [ 4775.5, 293.90000000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos33", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.445], "Focus": [17741.4], "XYStage": [ 3954.9, 293.90000000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos34", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.455], "Focus": [17741.4], "XYStage": [ -1324.9, 545.6 ] }, "GridColumnIndex": 0 }, { "Label": "Pos35", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.445], "Focus": [17741.3], "XYStage": [ -2097, 576.6 ] }, "GridColumnIndex": 0 } ], "z-step_um": 10, "GridRow": 0, "BitDepth": 16, "Depth": 2, "Channels": 6, "Source": "Micro-Manager", "ChColors": [ -16711681, -256, -65281, -1, -1, -16776961 ], "MetadataVersion": 10, "KeepShutterOpenChannels": true, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536, 65536 ], "CustomIntervals_ms": [], "Height": 1200, "MicroManagerVersion": "1.4.23 20180219", "GridColumn": 0, "UUID": "18808e7c-f2e6-47b3-90d8-1d779ce87dbd", "PixelType": "GRAY16", "Width": 1200, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\Yan\\20191218-YC113-S7S8-miseq", "Frames": 1, "Comment": "", "UserName": "Nikon", "PixelSize_um": 1.1, "ROI": [ 0, 0, 1200, 1200 ], "Date": "2019-12-19", "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC", "P-DAPI-high" ], "KeepShutterOpenSlices": true, "IJType": 1, "ComputerName": "ZadorConfocal2", "Positions": 36, "PositionIndex": 34, "CameraTimeout": 20000, "TimeFirst": false }, "640 AO-Name": "AnalogIO", "Vincent-D3-Command": "Close" }, "FrameKey-0-1-10": { "COM4-StopBits": "1", "528 AO-MaxVolts": "5.0000", "XLIGHT Excitation Wheel-State": "1", "470 Shutter-Name": "AnalogIO", "Core-Shutter": "555", "555 AO-Demo": "No", "XLIGHT Dichroic-State": "2", "XYStage-StepSize-Y": "0.1000", "ZPositionUm": 268.45500000000004, "XYStage-StepSize-X": "0.1000", "PRIME-FirmwareVersion": "20.28", "PRIME-ClearCycles": "2", "520-Name": "DA Shutter", "405 Shutter-Name": "AnalogIO", "PIEZO-StepSize": "0.0050", "PRIME-ClearMode": "Pre-Exposure", "470 AO-MinVolts": "0.0000", "405 AO-SupportsTriggering": "1", "CameraChannelIndex": 0, "XYStage-StartSpeed": "500.0000", "COM9-StopBits": "2", "405 AO-Description": "NI DAC", "COM4-DelayBetweenCharsMs": "0.0000", "445 AO-Block voltage": "false", "520 AO-TriggerInputLine": "/Dev1/PFI0", "555 AO-Description": "NI DAC", "520 AO-TriggerSampleRate": "100000", "COM4-Verbose": "1", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM4-Name": "COM4", "405 Shutter-Volts": "0.0000", "Vincent-D3-Port": "COM1", "PRIME-PP 4 MIN ADU AFFECTED": "200", "470 Shutter-MinVolts": "0.0000", "PRIME-PP 3 ENABLED": "Yes", "470 AO-Description": "NI DAC", "PRIME-Description": "PVCAM API device adapter", "520 Shutter-MaxVolts": "5.0000", "520 Shutter-Volts": "0.0000", "528 AO-Sequenceable": "1", "PRIME-Binning": "1x1", "555 Shutter-MaxVolts": "5.0000", "528 Shutter-TriggerSequenceLength": "1000", "405 Shutter-Block voltage": "false", "XLIGHT Spin motor-State": "1", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "Vincent-D3-Time to open (ms)": "35.0000", "Vincent-D3-Time to close (ms)": "35.0000", "445 AO-TriggerSampleRate": "100000", "640 Shutter-IOChannel": "Dev1/ao14", "555 AO-Sequenceable": "1", "520 AO-Name": "AnalogIO", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "470 AO-Block voltage": "false", "COM1-Verbose": "1", "COM9-AnswerTimeout": "10000.0000", "528 Shutter-Name": "AnalogIO", "PRIME-Trigger Timeout (secs)": "10", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "NextFrame": 0, "470 AO-TriggerSequenceLength": "1000", "PRIME-TransposeXY": "0", "445 AO-TriggerSequenceLength": "1000", "COM1-Name": "COM1", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "COM9-DataBits": "8", "COM1-AnswerTimeout": "500.0000", "Camera": "", "TE2000-Version": "V2.50-V2.50-V2.10", "528 Shutter-Volts": "0.0000", "XLIGHT Hub-Name": "XLIGHT Hub", "PIEZO-Description": "Ludl stage driver adapter", "PositionName": "Pos34", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSampleRate": "100000", "PRIME-FanSpeedSetpoint": "High", "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "405 AO-TriggerSampleRate": "100000", "470 Shutter-Block voltage": "false", "470-State": "0", "COM3-Parity": "None", "405 AO-Volts": "0.0000", "PRIME-TransposeMirrorY": "0", "PRIME-ChipName": "GS144BSI", "470 AO-Volts": "0.0000", "PRIME-TransposeMirrorX": "0", "445 Shutter-SupportsTriggering": "0", "470 Shutter-SupportsTriggering": "0", "XLIGHT Hub-Description": "XLIGHT hub", "PRIME-PreampOffLimit": "10000", "470 AO-Sequenceable": "1", "528 Shutter-MaxVolts": "5.0000", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "Core-XYStage": "XYStage", "470 AO-TriggerSampleRate": "100000", "PRIME-Timing-PreTriggerDelayNs": "24672000.0000", "528-DA Device": "528 Shutter", "Focus-Description": "Nikon TE2000 focus adapter", "FilterBlock-State": "5", "520 AO-Demo": "No", "COM3-DelayBetweenCharsMs": "0.0000", "470 Shutter-Volts": "0.0000", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "PRIME-PP 2 THRESHOLD": "125", "PRIME-PP 4 THRESHOLD": "75", "XYStage-Acceleration": "4.0000", "470 Shutter-Demo": "No", "PRIME-Name": "PMPCIECam00", "640-DA Device": "640 Shutter", "405 AO-Block voltage": "false", "ElapsedTime-ms": 1402233, "PRIME-Offset": "100", "445 AO-IOChannel": "Dev1/ao1", "640 AO-MaxVolts": "5.0000", "PositionIndex": 34, "PRIME-PP 1 ENABLED": "Yes", "COM1-BaudRate": "9600", "640-State": "0", "COM3-Verbose": "1", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "LudlController-TransmissionDelay": "0", "PRIME-FullWellCapacity": "62000", "PRIME-SMARTStreamingEnabled": "No", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "640 Shutter-Description": "NI DAC", "528-Description": "DA device that is used as a shutter", "COM4-Description": "Serial port driver (boost:asio)", "405 Shutter-Description": "NI DAC", "TE2000-Name": "TE2000", "528 Shutter-Description": "NI DAC", "COM9-Description": "Serial port driver (boost:asio)", "LudlController-Name": "LudlController", "445 Shutter-TriggerSequenceLength": "1000", "PRIME-SerialNumber": "A18B203008", "445 AO-Volts": "0.0000", "PRIME-X-dimension": "1200", "PRIME-ShutterCloseDelay": "0", "XLIGHT Disk slider-State": "2", "Position": "Default", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 AO-IOChannel": "Dev1/ao5", "520 AO-IOChannel": "Dev1/ao4", "528 AO-TriggerSequenceLength": "1000", "405 Shutter-MaxVolts": "5.0000", "470-DA Device": "470 Shutter", "PRIME-FTCapable": "No", "Core-ChannelGroup": "Channels", "555 AO-SupportsTriggering": "0", "PRIME-Y-dimension": "1200", "Frame": 0, "555 Shutter-Sequenceable": "1", "PRIME-Timing-ExposureTimeNs": "499998000.0000", "Core-ImageProcessor": "", "528-State": "0", "520 AO-Block voltage": "false", "XLIGHT Excitation Wheel-Label": "QUAD", "640 Shutter-Demo": "No", "COM3-StopBits": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "520 Shutter-IOChannel": "Dev1/ao11", "Width": 1200, "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-SupportsTriggering": "0", "640 AO-TriggerSampleRate": "100000", "520-Description": "DA device that is used as a shutter", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "405 Shutter-Sequenceable": "1", "405 Shutter-SupportsTriggering": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-TriggerSampleRate": "100000", "640 Shutter-MaxVolts": "5.0000", "555 AO-MaxVolts": "5.0000", "520 AO-TriggerSequenceLength": "1000", "COM3-BaudRate": "9600", "COM9-BaudRate": "9600", "Vincent-D3-Channel #": "Ch. #1", "PRIME-PMode": "Normal", "OpticalPath-Label": "Left(100)", "BitDepth": 16, "555 AO-TriggerSequenceLength": "1000", "445 Shutter-Description": "NI DAC", "405 AO-Sequenceable": "1", "PRIME-AcquisitionMethod": "Callbacks", "555 AO-Name": "AnalogIO", "PRIME-ShutterMode": "Pre-Exposure", "COM3-Handshaking": "Off", "445 AO-SupportsTriggering": "0", "ROI": "0-0-1200-1200", "COM9-Verbose": "1", "PRIME-Color": "OFF", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "PRIME-ShutterOpenDelay": "0", "COM1-Parity": "None", "Slice": 10, "SlicePosition": 50, "COM9-DelayBetweenCharsMs": "0.0000", "555-Name": "DA Shutter", "PIEZO-LudlSingleAxisName": "B", "XLIGHT Emission Wheel-Label": "585/11(T)", "640 Shutter-Name": "AnalogIO", "640 AO-Sequenceable": "1", "520 Shutter-Demo": "No", "528 Shutter-Demo": "No", "470-Name": "DA Shutter", "520-DA Device": "520 Shutter", "XYStage-Description": "Ludl XY stage driver adapter", "445 Shutter-MinVolts": "0.0000", "528 AO-TriggerSampleRate": "100000", "405 AO-MinVolts": "0.0000", "405 Shutter-TriggerSequenceLength": "1000", "Height": 1200, "555 AO-Block voltage": "false", "640 Shutter-Volts": "0.0000", "PixelType": "GRAY16", "445 Shutter-Block voltage": "false", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "520 Shutter-TriggerSequenceLength": "1000", "COM9-Name": "COM9", "Core-SLM": "", "555 AO-Volts": "4.5000", "555 Shutter-Block voltage": "false", "XYStage-Speed": "1800.0000", "520 AO-MaxVolts": "5.0000", "528 Shutter-SupportsTriggering": "0", "COM1-Description": "Serial port driver (boost:asio)", "PRIME-MetadataEnabled": "No", "520 Shutter-MinVolts": "0.0000", "470 Shutter-Sequenceable": "1", "PRIME-PreampDelay": "0", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-MinVolts": "0.0000", "ChannelIndex": 1, "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Core-AutoShutter": "1", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "Exposure-ms": 160, "PRIME-PP 2 ENABLED": "Yes", "640 Shutter-TriggerSampleRate": "100000", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "470 Shutter-IOChannel": "Dev1/ao10", "405 AO-Demo": "No", "PixelSizeUm": 1.1, "470 AO-Demo": "No", "470 Shutter-MaxVolts": "5.0000", "Vincent-D3-Name": "Vincent-D3", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FileName": "img_000000000_Il-T_010.tif", "LudlController-Reset": "Operate", "PRIME-Exposure": "160.00", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "445-Name": "DA Shutter", "555 Shutter-Name": "AnalogIO", "520 Shutter-TriggerSampleRate": "100000", "445 Shutter-IOChannel": "Dev1/ao9", "PRIME-TransposeCorrection": "0", "XYStage-StepSize": "0.1000", "445-Description": "DA device that is used as a shutter", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 AO-IOChannel": "Dev1/ao7", "520 Shutter-Name": "AnalogIO", "COM3-DataBits": "8", "640 AO-Volts": "0.0000", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "555 Shutter-IOChannel": "Dev1/ao13", "WaitInterval": null, "520 Shutter-Sequenceable": "1", "YPositionUm": 545.6, "445-State": "0", "445-DA Device": "445 Shutter", "PRIME-CircularBufferAutoSize": "ON", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "555 AO-TriggerSampleRate": "100000", "445 Shutter-Volts": "0.0000", "XLIGHT Hub-Port": "COM3", "Core-AutoFocus": "", "COM4-Handshaking": "Off", "528 Shutter-MinVolts": "0.0000", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "445 Shutter-Name": "AnalogIO", "528 Shutter-Sequenceable": "1", "XYStage-TransposeMirrorX": "0", "XYStage-TransposeMirrorY": "0", "PRIME-ReadoutRate": "100MHz 16bit", "XYStage-Name": "Stage", "640 AO-SupportsTriggering": "0", "640-Name": "DA Shutter", "640 Shutter-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "640 AO-Block voltage": "false", "445 Shutter-Sequenceable": "1", "470 AO-MaxVolts": "5.0000", "COM4-BaudRate": "9600", "470-Description": "DA device that is used as a shutter", "520 Shutter-Description": "NI DAC", "PRIME-CCDTemperature": "-14.8500", "Time": "2019-12-19 09:59:32 -0500", "640 AO-TriggerSequenceLength": "1000", "470 AO-Name": "AnalogIO", "XLIGHT Disk slider-Label": "Disk Pos 40um", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "Binning": "1x1", "TE2000-ModelType": "1", "COM3-AnswerTimeout": "500.0000", "555 Shutter-SupportsTriggering": "0", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "TE2000-Port": "COM4", "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Description": "NI DAC", "COM4-AnswerTimeout": "500.0000", "COM1-DelayBetweenCharsMs": "0.0000", "FilterBlock-Label": "Confocal", "445 AO-Name": "AnalogIO", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-Timing-ReadoutTimeNs": "24672000.0000", "528 AO-Demo": "No", "520 AO-Sequenceable": "1", "555-Description": "DA device that is used as a shutter", "405-DA Device": "405 Shutter", "405 AO-TriggerSequenceLength": "1000", "640 AO-MinVolts": "0.0000", "640 Shutter-Block voltage": "false", "FrameIndex": 0, "PRIME-Port": "CMOS", "555 AO-MinVolts": "0.0000", "COM3-Description": "Serial port driver (boost:asio)", "528 AO-Description": "NI DAC", "555 AO-IOChannel": "Dev1/ao6", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "555 AO-TriggerInputLine": "/Dev1/PFI0", "PRIME-ActualInterval-ms": "390.5200", "LudlController-Port": "COM9", "445 Shutter-Demo": "No", "PRIME-Trigger-Expose Out-Mux": "1", "SliceIndex": 10, "PRIME-CCDTemperatureSetPoint": "-15.0000", "405 Shutter-MinVolts": "0.0000", "PRIME-ExposeOutMode": "First Row", "AxisPositions": null, "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Volts": "5.0000", "528-Name": "DA Shutter", "555-State": "0", "XLIGHT Dichroic-Label": "QUAD", "445 AO-Sequenceable": "1", "405-Name": "DA Shutter", "405 AO-IOChannel": "Dev1/ao0", "Core-Camera": "PRIME", "Focus-Name": "Focus", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "405 Shutter-IOChannel": "Dev1/ao8", "640 AO-Demo": "No", "640 Shutter-SupportsTriggering": "0", "445 AO-Demo": "No", "555-DA Device": "555 Shutter", "470 Shutter-Description": "NI DAC", "528 AO-Volts": "0.0000", "COM1-DataBits": "8", "Vincent-D3-Address": "x", "520 AO-SupportsTriggering": "0", "405-Description": "DA device that is used as a shutter", "640 Shutter-MinVolts": "0.0000", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "405 AO-Name": "AnalogIO", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "640 AO-TriggerInputLine": "/Dev1/PFI0", "405 AO-TriggerInputLine": "/Dev1/PFI0", "640-Description": "DA device that is used as a shutter", "445 Shutter-TriggerSampleRate": "100000", "470 Shutter-TriggerSequenceLength": "1000", "COM1-StopBits": "1", "520 Shutter-SupportsTriggering": "0", "405-State": "0", "Core-Initialize": "1", "XLIGHT Emission Wheel-State": "3", "520 AO-Description": "NI DAC", "528 AO-TriggerInputLine": "/Dev1/PFI0", "445 Shutter-MaxVolts": "5.0000", "COM9-Handshaking": "Off", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "XLIGHT Spin motor-Label": "On (spinning)", "PRIME-PP 5 ENABLED": "No", "UUID": "8f9c26a4-39db-4e26-9648-975eb733df30", "PRIME-TriggerMode": "Internal Trigger", "COM1-Handshaking": "Off", "PRIME-Timing-PostTriggerDelayNs": "49344000.0000", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "Focus-Position": "17741.5000", "528 Shutter-Block voltage": "false", "Channel": "Il-T", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "555 Shutter-Demo": "No", "405 Shutter-Demo": "No", "528 AO-Name": "AnalogIO", "520-State": "0", "Core-Galvo": "", "405 Shutter-TriggerSampleRate": "100000", "Focus-FocusStepSize_(nm)": "100", "PRIME-PixelType": "16bit", "COM4-DataBits": "8", "470 AO-IOChannel": "Dev1/ao2", "520 AO-MinVolts": "0.0000", "COM9-Parity": "None", "PRIME-CircularBufferEnabled": "ON", "PRIME-PP 6 Reset": "No", "640 AO-Description": "NI DAC", "Source": "PRIME", "445 AO-MinVolts": "0.0000", "PIEZO-Name": "Stage", "445 AO-Description": "NI DAC", "PRIME-CircularBufferFrameCount": "46", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "520 AO-Volts": "0.0000", "528 Shutter-TriggerSampleRate": "100000", "XPositionUm": -1324.9, "OpticalPath-State": "4", "PRIME-PP 3 THRESHOLD": "80", "PRIME-PVCAM Version": "3.7.1", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Gain": "1-HDR", "640 Shutter-TriggerSequenceLength": "1000", "528 AO-SupportsTriggering": "0", "445 AO-TriggerInputLine": "/Dev1/PFI0", "OpticalPath-Name": "OpticalPath", "PRIME-PP 4 ENABLED": "Yes", "PRIME-PVCAM Adapter Version": "1.3.29", "COM3-Name": "COM3", "405 AO-MaxVolts": "5.0000", "520 Shutter-Block voltage": "false", "PRIME-Timing-ClearingTimeNs": "49344000.0000", "Core-Focus": "PIEZO", "528 AO-Block voltage": "false", "TE2000-Description": "Nikon TE2000 hub", "528 AO-MinVolts": "0.0000", "COM4-Parity": "None", "Summary": { "Slices": 11, "ChContrastMin": [ 0, 0, 0, 0, 0, 0 ], "PixelAspect": 1, "SlicesFirst": true, "Interval_ms": 0, "Time": "2019-12-19 09:59:21 -0500", "Prefix": "seq_13", "InitialPositionList": [ { "Label": "Pos0", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.21], "Focus": [17741.5], "XYStage": [ 44542.200000000004, 31636.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos1", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.22], "Focus": [17741.4], "XYStage": [ 43801.100000000006, 31637.9 ] }, "GridColumnIndex": 0 }, { "Label": "Pos2", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.5], "XYStage": [ 38561.3, 32040.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos3", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.185], "Focus": [17741.3], "XYStage": [ 37698.4, 32041.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos4", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.16], "Focus": [17741.5], "XYStage": [ 32375.4, 31758.9 ] }, "GridColumnIndex": 0 }, { "Label": "Pos5", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.17000000000002], "Focus": [17741.3], "XYStage": [ 31770.7, 31758.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos6", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.4], "XYStage": [ 26536.800000000003, 31068.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos7", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.205], "Focus": [17741.4], "XYStage": [ 25420.2, 31068.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos8", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.85], "Focus": [17741.3], "XYStage": [ 21059, 31508 ] }, "GridColumnIndex": 0 }, { "Label": "Pos9", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.82], "Focus": [17741.3], "XYStage": [ 19731.9, 31506.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos10", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.79], "Focus": [17741.4], "XYStage": [ 14528.5, 32007.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos11", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.83], "Focus": [17741.2], "XYStage": [ 13830.300000000001, 32009.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos12", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.79], "Focus": [17741.3], "XYStage": [ 8775.300000000001, 32017.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos13", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.815], "Focus": [17741.3], "XYStage": [ 8109.200000000001, 32008.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos14", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.805], "Focus": [17741.3], "XYStage": [ 3483.3, 31908.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos15", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.82], "Focus": [17741.2], "XYStage": [ 2686.8, 31903.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos16", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [211.48000000000002], "Focus": [17741.1], "XYStage": [ -1978.9, 31973.600000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos17", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [211.51500000000001], "Focus": [17741.1], "XYStage": [ -2957.8, 31982.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos18", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.195], "Focus": [17741.5], "XYStage": [ 47747.4, -576.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos19", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.23000000000002], "Focus": [17741.6], "XYStage": [ 47053.9, -576.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos20", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.205], "Focus": [17741.4], "XYStage": [ 41871.200000000004, -479.8 ] }, "GridColumnIndex": 0 }, { "Label": "Pos21", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.4], "XYStage": [ 40882.600000000006, -392 ] }, "GridColumnIndex": 0 }, { "Label": "Pos22", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.24], "Focus": [17741.4], "XYStage": [ 35905.6, -135.3 ] }, "GridColumnIndex": 0 }, { "Label": "Pos23", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.195], "Focus": [17741.5], "XYStage": [ 34990.9, -132.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos24", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.46], "Focus": [17741.4], "XYStage": [ 29678.100000000002, -958.1 ] }, "GridColumnIndex": 0 }, { "Label": "Pos25", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.44], "Focus": [17741.5], "XYStage": [ 28983.600000000002, -939.4000000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos26", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.475], "Focus": [17741.5], "XYStage": [ 23019.7, -122 ] }, "GridColumnIndex": 0 }, { "Label": "Pos27", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.44], "Focus": [17741.7], "XYStage": [ 22428, -116.80000000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos28", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.46], "Focus": [17741.7], "XYStage": [ 16364.900000000001, -181.10000000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos29", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.48000000000002], "Focus": [17741.5], "XYStage": [ 15712.1, -180 ] }, "GridColumnIndex": 0 }, { "Label": "Pos30", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.45000000000002], "Focus": [17741.7], "XYStage": [ 10346.1, 77.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos31", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.49], "Focus": [17741.4], "XYStage": [ 9755.5, 85.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos32", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.475], "Focus": [17741.5], "XYStage": [ 4775.5, 293.90000000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos33", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.445], "Focus": [17741.4], "XYStage": [ 3954.9, 293.90000000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos34", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.455], "Focus": [17741.4], "XYStage": [ -1324.9, 545.6 ] }, "GridColumnIndex": 0 }, { "Label": "Pos35", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.445], "Focus": [17741.3], "XYStage": [ -2097, 576.6 ] }, "GridColumnIndex": 0 } ], "z-step_um": 10, "GridRow": 0, "BitDepth": 16, "Depth": 2, "Channels": 6, "Source": "Micro-Manager", "ChColors": [ -16711681, -256, -65281, -1, -1, -16776961 ], "MetadataVersion": 10, "KeepShutterOpenChannels": true, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536, 65536 ], "CustomIntervals_ms": [], "Height": 1200, "MicroManagerVersion": "1.4.23 20180219", "GridColumn": 0, "UUID": "18808e7c-f2e6-47b3-90d8-1d779ce87dbd", "PixelType": "GRAY16", "Width": 1200, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\Yan\\20191218-YC113-S7S8-miseq", "Frames": 1, "Comment": "", "UserName": "Nikon", "PixelSize_um": 1.1, "ROI": [ 0, 0, 1200, 1200 ], "Date": "2019-12-19", "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC", "P-DAPI-high" ], "KeepShutterOpenSlices": true, "IJType": 1, "ComputerName": "ZadorConfocal2", "Positions": 36, "PositionIndex": 34, "CameraTimeout": 20000, "TimeFirst": false }, "640 AO-Name": "AnalogIO", "Vincent-D3-Command": "Close" }, "FrameKey-0-2-0": { "COM4-StopBits": "1", "528 AO-MaxVolts": "5.0000", "XLIGHT Excitation Wheel-State": "1", "470 Shutter-Name": "AnalogIO", "Core-Shutter": "640", "555 AO-Demo": "No", "XLIGHT Dichroic-State": "2", "XYStage-StepSize-Y": "0.1000", "ZPositionUm": 168.455, "XYStage-StepSize-X": "0.1000", "PRIME-FirmwareVersion": "20.28", "PRIME-ClearCycles": "2", "520-Name": "DA Shutter", "405 Shutter-Name": "AnalogIO", "PIEZO-StepSize": "0.0050", "PRIME-ClearMode": "Pre-Exposure", "470 AO-MinVolts": "0.0000", "405 AO-SupportsTriggering": "1", "CameraChannelIndex": 0, "XYStage-StartSpeed": "500.0000", "COM9-StopBits": "2", "405 AO-Description": "NI DAC", "COM4-DelayBetweenCharsMs": "0.0000", "445 AO-Block voltage": "false", "520 AO-TriggerInputLine": "/Dev1/PFI0", "555 AO-Description": "NI DAC", "520 AO-TriggerSampleRate": "100000", "COM4-Verbose": "1", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM4-Name": "COM4", "405 Shutter-Volts": "0.0000", "Vincent-D3-Port": "COM1", "PRIME-PP 4 MIN ADU AFFECTED": "200", "470 Shutter-MinVolts": "0.0000", "PRIME-PP 3 ENABLED": "Yes", "470 AO-Description": "NI DAC", "PRIME-Description": "PVCAM API device adapter", "520 Shutter-MaxVolts": "5.0000", "520 Shutter-Volts": "0.0000", "528 AO-Sequenceable": "1", "PRIME-Binning": "1x1", "555 Shutter-MaxVolts": "5.0000", "528 Shutter-TriggerSequenceLength": "1000", "405 Shutter-Block voltage": "false", "XLIGHT Spin motor-State": "1", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "Vincent-D3-Time to open (ms)": "35.0000", "Vincent-D3-Time to close (ms)": "35.0000", "445 AO-TriggerSampleRate": "100000", "640 Shutter-IOChannel": "Dev1/ao14", "555 AO-Sequenceable": "1", "520 AO-Name": "AnalogIO", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "470 AO-Block voltage": "false", "COM1-Verbose": "1", "COM9-AnswerTimeout": "10000.0000", "528 Shutter-Name": "AnalogIO", "PRIME-Trigger Timeout (secs)": "10", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "NextFrame": 0, "470 AO-TriggerSequenceLength": "1000", "PRIME-TransposeXY": "0", "445 AO-TriggerSequenceLength": "1000", "COM1-Name": "COM1", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "COM9-DataBits": "8", "COM1-AnswerTimeout": "500.0000", "Camera": "", "TE2000-Version": "V2.50-V2.50-V2.10", "528 Shutter-Volts": "0.0000", "XLIGHT Hub-Name": "XLIGHT Hub", "PIEZO-Description": "Ludl stage driver adapter", "PositionName": "Pos34", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSampleRate": "100000", "PRIME-FanSpeedSetpoint": "High", "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "405 AO-TriggerSampleRate": "100000", "470 Shutter-Block voltage": "false", "470-State": "0", "COM3-Parity": "None", "405 AO-Volts": "0.0000", "PRIME-TransposeMirrorY": "0", "PRIME-ChipName": "GS144BSI", "470 AO-Volts": "0.0000", "PRIME-TransposeMirrorX": "0", "445 Shutter-SupportsTriggering": "0", "470 Shutter-SupportsTriggering": "0", "XLIGHT Hub-Description": "XLIGHT hub", "PRIME-PreampOffLimit": "10000", "470 AO-Sequenceable": "1", "528 Shutter-MaxVolts": "5.0000", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "Core-XYStage": "XYStage", "470 AO-TriggerSampleRate": "100000", "PRIME-Timing-PreTriggerDelayNs": "24672000.0000", "528-DA Device": "528 Shutter", "Focus-Description": "Nikon TE2000 focus adapter", "FilterBlock-State": "5", "520 AO-Demo": "No", "COM3-DelayBetweenCharsMs": "0.0000", "470 Shutter-Volts": "0.0000", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "PRIME-PP 2 THRESHOLD": "125", "PRIME-PP 4 THRESHOLD": "75", "XYStage-Acceleration": "4.0000", "470 Shutter-Demo": "No", "PRIME-Name": "PMPCIECam00", "640-DA Device": "640 Shutter", "405 AO-Block voltage": "false", "ElapsedTime-ms": 1403348, "PRIME-Offset": "100", "445 AO-IOChannel": "Dev1/ao1", "640 AO-MaxVolts": "5.0000", "PositionIndex": 34, "PRIME-PP 1 ENABLED": "Yes", "COM1-BaudRate": "9600", "640-State": "1", "COM3-Verbose": "1", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "LudlController-TransmissionDelay": "0", "PRIME-FullWellCapacity": "62000", "PRIME-SMARTStreamingEnabled": "No", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "640 Shutter-Description": "NI DAC", "528-Description": "DA device that is used as a shutter", "COM4-Description": "Serial port driver (boost:asio)", "405 Shutter-Description": "NI DAC", "TE2000-Name": "TE2000", "528 Shutter-Description": "NI DAC", "COM9-Description": "Serial port driver (boost:asio)", "LudlController-Name": "LudlController", "445 Shutter-TriggerSequenceLength": "1000", "PRIME-SerialNumber": "A18B203008", "445 AO-Volts": "0.0000", "PRIME-X-dimension": "1200", "PRIME-ShutterCloseDelay": "0", "XLIGHT Disk slider-State": "2", "Position": "Default", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 AO-IOChannel": "Dev1/ao5", "520 AO-IOChannel": "Dev1/ao4", "528 AO-TriggerSequenceLength": "1000", "405 Shutter-MaxVolts": "5.0000", "470-DA Device": "470 Shutter", "PRIME-FTCapable": "No", "Core-ChannelGroup": "Channels", "555 AO-SupportsTriggering": "0", "PRIME-Y-dimension": "1200", "Frame": 0, "555 Shutter-Sequenceable": "1", "PRIME-Timing-ExposureTimeNs": "499998000.0000", "Core-ImageProcessor": "", "528-State": "0", "520 AO-Block voltage": "false", "XLIGHT Excitation Wheel-Label": "QUAD", "640 Shutter-Demo": "No", "COM3-StopBits": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "520 Shutter-IOChannel": "Dev1/ao11", "Width": 1200, "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-SupportsTriggering": "0", "640 AO-TriggerSampleRate": "100000", "520-Description": "DA device that is used as a shutter", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "405 Shutter-Sequenceable": "1", "405 Shutter-SupportsTriggering": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-TriggerSampleRate": "100000", "640 Shutter-MaxVolts": "5.0000", "555 AO-MaxVolts": "5.0000", "520 AO-TriggerSequenceLength": "1000", "COM3-BaudRate": "9600", "COM9-BaudRate": "9600", "Vincent-D3-Channel #": "Ch. #1", "PRIME-PMode": "Normal", "OpticalPath-Label": "Left(100)", "BitDepth": 16, "555 AO-TriggerSequenceLength": "1000", "445 Shutter-Description": "NI DAC", "405 AO-Sequenceable": "1", "PRIME-AcquisitionMethod": "Callbacks", "555 AO-Name": "AnalogIO", "PRIME-ShutterMode": "Pre-Exposure", "COM3-Handshaking": "Off", "445 AO-SupportsTriggering": "0", "ROI": "0-0-1200-1200", "COM9-Verbose": "1", "PRIME-Color": "OFF", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "PRIME-ShutterOpenDelay": "0", "COM1-Parity": "None", "Slice": 0, "SlicePosition": -50, "COM9-DelayBetweenCharsMs": "0.0000", "555-Name": "DA Shutter", "PIEZO-LudlSingleAxisName": "B", "XLIGHT Emission Wheel-Label": "679/29 (A)", "640 Shutter-Name": "AnalogIO", "640 AO-Sequenceable": "1", "520 Shutter-Demo": "No", "528 Shutter-Demo": "No", "470-Name": "DA Shutter", "520-DA Device": "520 Shutter", "XYStage-Description": "Ludl XY stage driver adapter", "445 Shutter-MinVolts": "0.0000", "528 AO-TriggerSampleRate": "100000", "405 AO-MinVolts": "0.0000", "405 Shutter-TriggerSequenceLength": "1000", "Height": 1200, "555 AO-Block voltage": "false", "640 Shutter-Volts": "5.0000", "PixelType": "GRAY16", "445 Shutter-Block voltage": "false", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "520 Shutter-TriggerSequenceLength": "1000", "COM9-Name": "COM9", "Core-SLM": "", "555 AO-Volts": "0.0000", "555 Shutter-Block voltage": "false", "XYStage-Speed": "1800.0000", "520 AO-MaxVolts": "5.0000", "528 Shutter-SupportsTriggering": "0", "COM1-Description": "Serial port driver (boost:asio)", "PRIME-MetadataEnabled": "No", "520 Shutter-MinVolts": "0.0000", "470 Shutter-Sequenceable": "1", "PRIME-PreampDelay": "0", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-MinVolts": "0.0000", "ChannelIndex": 2, "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Core-AutoShutter": "1", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "Exposure-ms": 320, "PRIME-PP 2 ENABLED": "Yes", "640 Shutter-TriggerSampleRate": "100000", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "470 Shutter-IOChannel": "Dev1/ao10", "405 AO-Demo": "No", "PixelSizeUm": 1.1, "470 AO-Demo": "No", "470 Shutter-MaxVolts": "5.0000", "Vincent-D3-Name": "Vincent-D3", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FileName": "img_000000000_Il-A_000.tif", "LudlController-Reset": "Operate", "PRIME-Exposure": "320.00", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "445-Name": "DA Shutter", "555 Shutter-Name": "AnalogIO", "520 Shutter-TriggerSampleRate": "100000", "445 Shutter-IOChannel": "Dev1/ao9", "PRIME-TransposeCorrection": "0", "XYStage-StepSize": "0.1000", "445-Description": "DA device that is used as a shutter", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 AO-IOChannel": "Dev1/ao7", "520 Shutter-Name": "AnalogIO", "COM3-DataBits": "8", "640 AO-Volts": "4.5000", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "555 Shutter-IOChannel": "Dev1/ao13", "WaitInterval": null, "520 Shutter-Sequenceable": "1", "YPositionUm": 545.6, "445-State": "0", "445-DA Device": "445 Shutter", "PRIME-CircularBufferAutoSize": "ON", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "555 AO-TriggerSampleRate": "100000", "445 Shutter-Volts": "0.0000", "XLIGHT Hub-Port": "COM3", "Core-AutoFocus": "", "COM4-Handshaking": "Off", "528 Shutter-MinVolts": "0.0000", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "445 Shutter-Name": "AnalogIO", "528 Shutter-Sequenceable": "1", "XYStage-TransposeMirrorX": "0", "XYStage-TransposeMirrorY": "0", "PRIME-ReadoutRate": "100MHz 16bit", "XYStage-Name": "Stage", "640 AO-SupportsTriggering": "0", "640-Name": "DA Shutter", "640 Shutter-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "640 AO-Block voltage": "false", "445 Shutter-Sequenceable": "1", "470 AO-MaxVolts": "5.0000", "COM4-BaudRate": "9600", "470-Description": "DA device that is used as a shutter", "520 Shutter-Description": "NI DAC", "PRIME-CCDTemperature": "-14.8500", "Time": "2019-12-19 09:59:33 -0500", "640 AO-TriggerSequenceLength": "1000", "470 AO-Name": "AnalogIO", "XLIGHT Disk slider-Label": "Disk Pos 40um", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "Binning": "1x1", "TE2000-ModelType": "1", "COM3-AnswerTimeout": "500.0000", "555 Shutter-SupportsTriggering": "0", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "TE2000-Port": "COM4", "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Description": "NI DAC", "COM4-AnswerTimeout": "500.0000", "COM1-DelayBetweenCharsMs": "0.0000", "FilterBlock-Label": "Confocal", "445 AO-Name": "AnalogIO", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-Timing-ReadoutTimeNs": "24672000.0000", "528 AO-Demo": "No", "520 AO-Sequenceable": "1", "555-Description": "DA device that is used as a shutter", "405-DA Device": "405 Shutter", "405 AO-TriggerSequenceLength": "1000", "640 AO-MinVolts": "0.0000", "640 Shutter-Block voltage": "false", "FrameIndex": 0, "PRIME-Port": "CMOS", "555 AO-MinVolts": "0.0000", "COM3-Description": "Serial port driver (boost:asio)", "528 AO-Description": "NI DAC", "555 AO-IOChannel": "Dev1/ao6", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "555 AO-TriggerInputLine": "/Dev1/PFI0", "PRIME-ActualInterval-ms": "390.5200", "LudlController-Port": "COM9", "445 Shutter-Demo": "No", "PRIME-Trigger-Expose Out-Mux": "1", "SliceIndex": 0, "PRIME-CCDTemperatureSetPoint": "-15.0000", "405 Shutter-MinVolts": "0.0000", "PRIME-ExposeOutMode": "First Row", "AxisPositions": null, "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Volts": "0.0000", "528-Name": "DA Shutter", "555-State": "0", "XLIGHT Dichroic-Label": "Di03-R635-t1", "445 AO-Sequenceable": "1", "405-Name": "DA Shutter", "405 AO-IOChannel": "Dev1/ao0", "Core-Camera": "PRIME", "Focus-Name": "Focus", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "405 Shutter-IOChannel": "Dev1/ao8", "640 AO-Demo": "No", "640 Shutter-SupportsTriggering": "0", "445 AO-Demo": "No", "555-DA Device": "555 Shutter", "470 Shutter-Description": "NI DAC", "528 AO-Volts": "0.0000", "COM1-DataBits": "8", "Vincent-D3-Address": "x", "520 AO-SupportsTriggering": "0", "405-Description": "DA device that is used as a shutter", "640 Shutter-MinVolts": "0.0000", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "405 AO-Name": "AnalogIO", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "640 AO-TriggerInputLine": "/Dev1/PFI0", "405 AO-TriggerInputLine": "/Dev1/PFI0", "640-Description": "DA device that is used as a shutter", "445 Shutter-TriggerSampleRate": "100000", "470 Shutter-TriggerSequenceLength": "1000", "COM1-StopBits": "1", "520 Shutter-SupportsTriggering": "0", "405-State": "0", "Core-Initialize": "1", "XLIGHT Emission Wheel-State": "3", "520 AO-Description": "NI DAC", "528 AO-TriggerInputLine": "/Dev1/PFI0", "445 Shutter-MaxVolts": "5.0000", "COM9-Handshaking": "Off", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "XLIGHT Spin motor-Label": "On (spinning)", "PRIME-PP 5 ENABLED": "No", "UUID": "135a26d4-f1c3-4359-b9e6-7beda7223552", "PRIME-TriggerMode": "Internal Trigger", "COM1-Handshaking": "Off", "PRIME-Timing-PostTriggerDelayNs": "49344000.0000", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "Focus-Position": "17741.5000", "528 Shutter-Block voltage": "false", "Channel": "Il-A", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "555 Shutter-Demo": "No", "405 Shutter-Demo": "No", "528 AO-Name": "AnalogIO", "520-State": "0", "Core-Galvo": "", "405 Shutter-TriggerSampleRate": "100000", "Focus-FocusStepSize_(nm)": "100", "PRIME-PixelType": "16bit", "COM4-DataBits": "8", "470 AO-IOChannel": "Dev1/ao2", "520 AO-MinVolts": "0.0000", "COM9-Parity": "None", "PRIME-CircularBufferEnabled": "ON", "PRIME-PP 6 Reset": "No", "640 AO-Description": "NI DAC", "Source": "PRIME", "445 AO-MinVolts": "0.0000", "PIEZO-Name": "Stage", "445 AO-Description": "NI DAC", "PRIME-CircularBufferFrameCount": "46", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "520 AO-Volts": "0.0000", "528 Shutter-TriggerSampleRate": "100000", "XPositionUm": -1324.9, "OpticalPath-State": "4", "PRIME-PP 3 THRESHOLD": "80", "PRIME-PVCAM Version": "3.7.1", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Gain": "1-HDR", "640 Shutter-TriggerSequenceLength": "1000", "528 AO-SupportsTriggering": "0", "445 AO-TriggerInputLine": "/Dev1/PFI0", "OpticalPath-Name": "OpticalPath", "PRIME-PP 4 ENABLED": "Yes", "PRIME-PVCAM Adapter Version": "1.3.29", "COM3-Name": "COM3", "405 AO-MaxVolts": "5.0000", "520 Shutter-Block voltage": "false", "PRIME-Timing-ClearingTimeNs": "49344000.0000", "Core-Focus": "PIEZO", "528 AO-Block voltage": "false", "TE2000-Description": "Nikon TE2000 hub", "528 AO-MinVolts": "0.0000", "COM4-Parity": "None", "Summary": { "Slices": 11, "ChContrastMin": [ 0, 0, 0, 0, 0, 0 ], "PixelAspect": 1, "SlicesFirst": true, "Interval_ms": 0, "Time": "2019-12-19 09:59:21 -0500", "Prefix": "seq_13", "InitialPositionList": [ { "Label": "Pos0", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.21], "Focus": [17741.5], "XYStage": [ 44542.200000000004, 31636.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos1", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.22], "Focus": [17741.4], "XYStage": [ 43801.100000000006, 31637.9 ] }, "GridColumnIndex": 0 }, { "Label": "Pos2", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.5], "XYStage": [ 38561.3, 32040.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos3", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.185], "Focus": [17741.3], "XYStage": [ 37698.4, 32041.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos4", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.16], "Focus": [17741.5], "XYStage": [ 32375.4, 31758.9 ] }, "GridColumnIndex": 0 }, { "Label": "Pos5", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.17000000000002], "Focus": [17741.3], "XYStage": [ 31770.7, 31758.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos6", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.4], "XYStage": [ 26536.800000000003, 31068.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos7", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.205], "Focus": [17741.4], "XYStage": [ 25420.2, 31068.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos8", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.85], "Focus": [17741.3], "XYStage": [ 21059, 31508 ] }, "GridColumnIndex": 0 }, { "Label": "Pos9", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.82], "Focus": [17741.3], "XYStage": [ 19731.9, 31506.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos10", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.79], "Focus": [17741.4], "XYStage": [ 14528.5, 32007.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos11", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.83], "Focus": [17741.2], "XYStage": [ 13830.300000000001, 32009.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos12", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.79], "Focus": [17741.3], "XYStage": [ 8775.300000000001, 32017.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos13", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.815], "Focus": [17741.3], "XYStage": [ 8109.200000000001, 32008.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos14", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.805], "Focus": [17741.3], "XYStage": [ 3483.3, 31908.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos15", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.82], "Focus": [17741.2], "XYStage": [ 2686.8, 31903.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos16", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [211.48000000000002], "Focus": [17741.1], "XYStage": [ -1978.9, 31973.600000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos17", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [211.51500000000001], "Focus": [17741.1], "XYStage": [ -2957.8, 31982.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos18", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.195], "Focus": [17741.5], "XYStage": [ 47747.4, -576.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos19", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.23000000000002], "Focus": [17741.6], "XYStage": [ 47053.9, -576.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos20", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.205], "Focus": [17741.4], "XYStage": [ 41871.200000000004, -479.8 ] }, "GridColumnIndex": 0 }, { "Label": "Pos21", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.4], "XYStage": [ 40882.600000000006, -392 ] }, "GridColumnIndex": 0 }, { "Label": "Pos22", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.24], "Focus": [17741.4], "XYStage": [ 35905.6, -135.3 ] }, "GridColumnIndex": 0 }, { "Label": "Pos23", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.195], "Focus": [17741.5], "XYStage": [ 34990.9, -132.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos24", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.46], "Focus": [17741.4], "XYStage": [ 29678.100000000002, -958.1 ] }, "GridColumnIndex": 0 }, { "Label": "Pos25", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.44], "Focus": [17741.5], "XYStage": [ 28983.600000000002, -939.4000000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos26", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.475], "Focus": [17741.5], "XYStage": [ 23019.7, -122 ] }, "GridColumnIndex": 0 }, { "Label": "Pos27", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.44], "Focus": [17741.7], "XYStage": [ 22428, -116.80000000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos28", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.46], "Focus": [17741.7], "XYStage": [ 16364.900000000001, -181.10000000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos29", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.48000000000002], "Focus": [17741.5], "XYStage": [ 15712.1, -180 ] }, "GridColumnIndex": 0 }, { "Label": "Pos30", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.45000000000002], "Focus": [17741.7], "XYStage": [ 10346.1, 77.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos31", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.49], "Focus": [17741.4], "XYStage": [ 9755.5, 85.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos32", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.475], "Focus": [17741.5], "XYStage": [ 4775.5, 293.90000000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos33", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.445], "Focus": [17741.4], "XYStage": [ 3954.9, 293.90000000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos34", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.455], "Focus": [17741.4], "XYStage": [ -1324.9, 545.6 ] }, "GridColumnIndex": 0 }, { "Label": "Pos35", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.445], "Focus": [17741.3], "XYStage": [ -2097, 576.6 ] }, "GridColumnIndex": 0 } ], "z-step_um": 10, "GridRow": 0, "BitDepth": 16, "Depth": 2, "Channels": 6, "Source": "Micro-Manager", "ChColors": [ -16711681, -256, -65281, -1, -1, -16776961 ], "MetadataVersion": 10, "KeepShutterOpenChannels": true, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536, 65536 ], "CustomIntervals_ms": [], "Height": 1200, "MicroManagerVersion": "1.4.23 20180219", "GridColumn": 0, "UUID": "18808e7c-f2e6-47b3-90d8-1d779ce87dbd", "PixelType": "GRAY16", "Width": 1200, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\Yan\\20191218-YC113-S7S8-miseq", "Frames": 1, "Comment": "", "UserName": "Nikon", "PixelSize_um": 1.1, "ROI": [ 0, 0, 1200, 1200 ], "Date": "2019-12-19", "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC", "P-DAPI-high" ], "KeepShutterOpenSlices": true, "IJType": 1, "ComputerName": "ZadorConfocal2", "Positions": 36, "PositionIndex": 34, "CameraTimeout": 20000, "TimeFirst": false }, "640 AO-Name": "AnalogIO", "Vincent-D3-Command": "Close" }, "FrameKey-0-2-1": { "COM4-StopBits": "1", "528 AO-MaxVolts": "5.0000", "XLIGHT Excitation Wheel-State": "1", "470 Shutter-Name": "AnalogIO", "Core-Shutter": "640", "555 AO-Demo": "No", "XLIGHT Dichroic-State": "2", "XYStage-StepSize-Y": "0.1000", "ZPositionUm": 178.455, "XYStage-StepSize-X": "0.1000", "PRIME-FirmwareVersion": "20.28", "PRIME-ClearCycles": "2", "520-Name": "DA Shutter", "405 Shutter-Name": "AnalogIO", "PIEZO-StepSize": "0.0050", "PRIME-ClearMode": "Pre-Exposure", "470 AO-MinVolts": "0.0000", "405 AO-SupportsTriggering": "1", "CameraChannelIndex": 0, "XYStage-StartSpeed": "500.0000", "COM9-StopBits": "2", "405 AO-Description": "NI DAC", "COM4-DelayBetweenCharsMs": "0.0000", "445 AO-Block voltage": "false", "520 AO-TriggerInputLine": "/Dev1/PFI0", "555 AO-Description": "NI DAC", "520 AO-TriggerSampleRate": "100000", "COM4-Verbose": "1", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM4-Name": "COM4", "405 Shutter-Volts": "0.0000", "Vincent-D3-Port": "COM1", "PRIME-PP 4 MIN ADU AFFECTED": "200", "470 Shutter-MinVolts": "0.0000", "PRIME-PP 3 ENABLED": "Yes", "470 AO-Description": "NI DAC", "PRIME-Description": "PVCAM API device adapter", "520 Shutter-MaxVolts": "5.0000", "520 Shutter-Volts": "0.0000", "528 AO-Sequenceable": "1", "PRIME-Binning": "1x1", "555 Shutter-MaxVolts": "5.0000", "528 Shutter-TriggerSequenceLength": "1000", "405 Shutter-Block voltage": "false", "XLIGHT Spin motor-State": "1", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "Vincent-D3-Time to open (ms)": "35.0000", "Vincent-D3-Time to close (ms)": "35.0000", "445 AO-TriggerSampleRate": "100000", "640 Shutter-IOChannel": "Dev1/ao14", "555 AO-Sequenceable": "1", "520 AO-Name": "AnalogIO", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "470 AO-Block voltage": "false", "COM1-Verbose": "1", "COM9-AnswerTimeout": "10000.0000", "528 Shutter-Name": "AnalogIO", "PRIME-Trigger Timeout (secs)": "10", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "NextFrame": 0, "470 AO-TriggerSequenceLength": "1000", "PRIME-TransposeXY": "0", "445 AO-TriggerSequenceLength": "1000", "COM1-Name": "COM1", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "COM9-DataBits": "8", "COM1-AnswerTimeout": "500.0000", "Camera": "", "TE2000-Version": "V2.50-V2.50-V2.10", "528 Shutter-Volts": "0.0000", "XLIGHT Hub-Name": "XLIGHT Hub", "PIEZO-Description": "Ludl stage driver adapter", "PositionName": "Pos34", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSampleRate": "100000", "PRIME-FanSpeedSetpoint": "High", "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "405 AO-TriggerSampleRate": "100000", "470 Shutter-Block voltage": "false", "470-State": "0", "COM3-Parity": "None", "405 AO-Volts": "0.0000", "PRIME-TransposeMirrorY": "0", "PRIME-ChipName": "GS144BSI", "470 AO-Volts": "0.0000", "PRIME-TransposeMirrorX": "0", "445 Shutter-SupportsTriggering": "0", "470 Shutter-SupportsTriggering": "0", "XLIGHT Hub-Description": "XLIGHT hub", "PRIME-PreampOffLimit": "10000", "470 AO-Sequenceable": "1", "528 Shutter-MaxVolts": "5.0000", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "Core-XYStage": "XYStage", "470 AO-TriggerSampleRate": "100000", "PRIME-Timing-PreTriggerDelayNs": "24672000.0000", "528-DA Device": "528 Shutter", "Focus-Description": "Nikon TE2000 focus adapter", "FilterBlock-State": "5", "520 AO-Demo": "No", "COM3-DelayBetweenCharsMs": "0.0000", "470 Shutter-Volts": "0.0000", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "PRIME-PP 2 THRESHOLD": "125", "PRIME-PP 4 THRESHOLD": "75", "XYStage-Acceleration": "4.0000", "470 Shutter-Demo": "No", "PRIME-Name": "PMPCIECam00", "640-DA Device": "640 Shutter", "405 AO-Block voltage": "false", "ElapsedTime-ms": 1403908, "PRIME-Offset": "100", "445 AO-IOChannel": "Dev1/ao1", "640 AO-MaxVolts": "5.0000", "PositionIndex": 34, "PRIME-PP 1 ENABLED": "Yes", "COM1-BaudRate": "9600", "640-State": "1", "COM3-Verbose": "1", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "LudlController-TransmissionDelay": "0", "PRIME-FullWellCapacity": "62000", "PRIME-SMARTStreamingEnabled": "No", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "640 Shutter-Description": "NI DAC", "528-Description": "DA device that is used as a shutter", "COM4-Description": "Serial port driver (boost:asio)", "405 Shutter-Description": "NI DAC", "TE2000-Name": "TE2000", "528 Shutter-Description": "NI DAC", "COM9-Description": "Serial port driver (boost:asio)", "LudlController-Name": "LudlController", "445 Shutter-TriggerSequenceLength": "1000", "PRIME-SerialNumber": "A18B203008", "445 AO-Volts": "0.0000", "PRIME-X-dimension": "1200", "PRIME-ShutterCloseDelay": "0", "XLIGHT Disk slider-State": "2", "Position": "Default", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 AO-IOChannel": "Dev1/ao5", "520 AO-IOChannel": "Dev1/ao4", "528 AO-TriggerSequenceLength": "1000", "405 Shutter-MaxVolts": "5.0000", "470-DA Device": "470 Shutter", "PRIME-FTCapable": "No", "Core-ChannelGroup": "Channels", "555 AO-SupportsTriggering": "0", "PRIME-Y-dimension": "1200", "Frame": 0, "555 Shutter-Sequenceable": "1", "PRIME-Timing-ExposureTimeNs": "499998000.0000", "Core-ImageProcessor": "", "528-State": "0", "520 AO-Block voltage": "false", "XLIGHT Excitation Wheel-Label": "QUAD", "640 Shutter-Demo": "No", "COM3-StopBits": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "520 Shutter-IOChannel": "Dev1/ao11", "Width": 1200, "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-SupportsTriggering": "0", "640 AO-TriggerSampleRate": "100000", "520-Description": "DA device that is used as a shutter", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "405 Shutter-Sequenceable": "1", "405 Shutter-SupportsTriggering": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-TriggerSampleRate": "100000", "640 Shutter-MaxVolts": "5.0000", "555 AO-MaxVolts": "5.0000", "520 AO-TriggerSequenceLength": "1000", "COM3-BaudRate": "9600", "COM9-BaudRate": "9600", "Vincent-D3-Channel #": "Ch. #1", "PRIME-PMode": "Normal", "OpticalPath-Label": "Left(100)", "BitDepth": 16, "555 AO-TriggerSequenceLength": "1000", "445 Shutter-Description": "NI DAC", "405 AO-Sequenceable": "1", "PRIME-AcquisitionMethod": "Callbacks", "555 AO-Name": "AnalogIO", "PRIME-ShutterMode": "Pre-Exposure", "COM3-Handshaking": "Off", "445 AO-SupportsTriggering": "0", "ROI": "0-0-1200-1200", "COM9-Verbose": "1", "PRIME-Color": "OFF", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "PRIME-ShutterOpenDelay": "0", "COM1-Parity": "None", "Slice": 1, "SlicePosition": -40, "COM9-DelayBetweenCharsMs": "0.0000", "555-Name": "DA Shutter", "PIEZO-LudlSingleAxisName": "B", "XLIGHT Emission Wheel-Label": "679/29 (A)", "640 Shutter-Name": "AnalogIO", "640 AO-Sequenceable": "1", "520 Shutter-Demo": "No", "528 Shutter-Demo": "No", "470-Name": "DA Shutter", "520-DA Device": "520 Shutter", "XYStage-Description": "Ludl XY stage driver adapter", "445 Shutter-MinVolts": "0.0000", "528 AO-TriggerSampleRate": "100000", "405 AO-MinVolts": "0.0000", "405 Shutter-TriggerSequenceLength": "1000", "Height": 1200, "555 AO-Block voltage": "false", "640 Shutter-Volts": "5.0000", "PixelType": "GRAY16", "445 Shutter-Block voltage": "false", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "520 Shutter-TriggerSequenceLength": "1000", "COM9-Name": "COM9", "Core-SLM": "", "555 AO-Volts": "0.0000", "555 Shutter-Block voltage": "false", "XYStage-Speed": "1800.0000", "520 AO-MaxVolts": "5.0000", "528 Shutter-SupportsTriggering": "0", "COM1-Description": "Serial port driver (boost:asio)", "PRIME-MetadataEnabled": "No", "520 Shutter-MinVolts": "0.0000", "470 Shutter-Sequenceable": "1", "PRIME-PreampDelay": "0", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-MinVolts": "0.0000", "ChannelIndex": 2, "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Core-AutoShutter": "1", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "Exposure-ms": 320, "PRIME-PP 2 ENABLED": "Yes", "640 Shutter-TriggerSampleRate": "100000", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "470 Shutter-IOChannel": "Dev1/ao10", "405 AO-Demo": "No", "PixelSizeUm": 1.1, "470 AO-Demo": "No", "470 Shutter-MaxVolts": "5.0000", "Vincent-D3-Name": "Vincent-D3", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FileName": "img_000000000_Il-A_001.tif", "LudlController-Reset": "Operate", "PRIME-Exposure": "320.00", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "445-Name": "DA Shutter", "555 Shutter-Name": "AnalogIO", "520 Shutter-TriggerSampleRate": "100000", "445 Shutter-IOChannel": "Dev1/ao9", "PRIME-TransposeCorrection": "0", "XYStage-StepSize": "0.1000", "445-Description": "DA device that is used as a shutter", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 AO-IOChannel": "Dev1/ao7", "520 Shutter-Name": "AnalogIO", "COM3-DataBits": "8", "640 AO-Volts": "4.5000", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "555 Shutter-IOChannel": "Dev1/ao13", "WaitInterval": null, "520 Shutter-Sequenceable": "1", "YPositionUm": 545.6, "445-State": "0", "445-DA Device": "445 Shutter", "PRIME-CircularBufferAutoSize": "ON", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "555 AO-TriggerSampleRate": "100000", "445 Shutter-Volts": "0.0000", "XLIGHT Hub-Port": "COM3", "Core-AutoFocus": "", "COM4-Handshaking": "Off", "528 Shutter-MinVolts": "0.0000", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "445 Shutter-Name": "AnalogIO", "528 Shutter-Sequenceable": "1", "XYStage-TransposeMirrorX": "0", "XYStage-TransposeMirrorY": "0", "PRIME-ReadoutRate": "100MHz 16bit", "XYStage-Name": "Stage", "640 AO-SupportsTriggering": "0", "640-Name": "DA Shutter", "640 Shutter-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "640 AO-Block voltage": "false", "445 Shutter-Sequenceable": "1", "470 AO-MaxVolts": "5.0000", "COM4-BaudRate": "9600", "470-Description": "DA device that is used as a shutter", "520 Shutter-Description": "NI DAC", "PRIME-CCDTemperature": "-14.8500", "Time": "2019-12-19 09:59:34 -0500", "640 AO-TriggerSequenceLength": "1000", "470 AO-Name": "AnalogIO", "XLIGHT Disk slider-Label": "Disk Pos 40um", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "Binning": "1x1", "TE2000-ModelType": "1", "COM3-AnswerTimeout": "500.0000", "555 Shutter-SupportsTriggering": "0", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "TE2000-Port": "COM4", "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Description": "NI DAC", "COM4-AnswerTimeout": "500.0000", "COM1-DelayBetweenCharsMs": "0.0000", "FilterBlock-Label": "Confocal", "445 AO-Name": "AnalogIO", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-Timing-ReadoutTimeNs": "24672000.0000", "528 AO-Demo": "No", "520 AO-Sequenceable": "1", "555-Description": "DA device that is used as a shutter", "405-DA Device": "405 Shutter", "405 AO-TriggerSequenceLength": "1000", "640 AO-MinVolts": "0.0000", "640 Shutter-Block voltage": "false", "FrameIndex": 0, "PRIME-Port": "CMOS", "555 AO-MinVolts": "0.0000", "COM3-Description": "Serial port driver (boost:asio)", "528 AO-Description": "NI DAC", "555 AO-IOChannel": "Dev1/ao6", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "555 AO-TriggerInputLine": "/Dev1/PFI0", "PRIME-ActualInterval-ms": "390.5200", "LudlController-Port": "COM9", "445 Shutter-Demo": "No", "PRIME-Trigger-Expose Out-Mux": "1", "SliceIndex": 1, "PRIME-CCDTemperatureSetPoint": "-15.0000", "405 Shutter-MinVolts": "0.0000", "PRIME-ExposeOutMode": "First Row", "AxisPositions": null, "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Volts": "0.0000", "528-Name": "DA Shutter", "555-State": "0", "XLIGHT Dichroic-Label": "Di03-R635-t1", "445 AO-Sequenceable": "1", "405-Name": "DA Shutter", "405 AO-IOChannel": "Dev1/ao0", "Core-Camera": "PRIME", "Focus-Name": "Focus", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "405 Shutter-IOChannel": "Dev1/ao8", "640 AO-Demo": "No", "640 Shutter-SupportsTriggering": "0", "445 AO-Demo": "No", "555-DA Device": "555 Shutter", "470 Shutter-Description": "NI DAC", "528 AO-Volts": "0.0000", "COM1-DataBits": "8", "Vincent-D3-Address": "x", "520 AO-SupportsTriggering": "0", "405-Description": "DA device that is used as a shutter", "640 Shutter-MinVolts": "0.0000", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "405 AO-Name": "AnalogIO", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "640 AO-TriggerInputLine": "/Dev1/PFI0", "405 AO-TriggerInputLine": "/Dev1/PFI0", "640-Description": "DA device that is used as a shutter", "445 Shutter-TriggerSampleRate": "100000", "470 Shutter-TriggerSequenceLength": "1000", "COM1-StopBits": "1", "520 Shutter-SupportsTriggering": "0", "405-State": "0", "Core-Initialize": "1", "XLIGHT Emission Wheel-State": "3", "520 AO-Description": "NI DAC", "528 AO-TriggerInputLine": "/Dev1/PFI0", "445 Shutter-MaxVolts": "5.0000", "COM9-Handshaking": "Off", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "XLIGHT Spin motor-Label": "On (spinning)", "PRIME-PP 5 ENABLED": "No", "UUID": "464891db-3c63-4aab-a02b-e4eaccc6fb1b", "PRIME-TriggerMode": "Internal Trigger", "COM1-Handshaking": "Off", "PRIME-Timing-PostTriggerDelayNs": "49344000.0000", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "Focus-Position": "17741.5000", "528 Shutter-Block voltage": "false", "Channel": "Il-A", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "555 Shutter-Demo": "No", "405 Shutter-Demo": "No", "528 AO-Name": "AnalogIO", "520-State": "0", "Core-Galvo": "", "405 Shutter-TriggerSampleRate": "100000", "Focus-FocusStepSize_(nm)": "100", "PRIME-PixelType": "16bit", "COM4-DataBits": "8", "470 AO-IOChannel": "Dev1/ao2", "520 AO-MinVolts": "0.0000", "COM9-Parity": "None", "PRIME-CircularBufferEnabled": "ON", "PRIME-PP 6 Reset": "No", "640 AO-Description": "NI DAC", "Source": "PRIME", "445 AO-MinVolts": "0.0000", "PIEZO-Name": "Stage", "445 AO-Description": "NI DAC", "PRIME-CircularBufferFrameCount": "46", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "520 AO-Volts": "0.0000", "528 Shutter-TriggerSampleRate": "100000", "XPositionUm": -1324.9, "OpticalPath-State": "4", "PRIME-PP 3 THRESHOLD": "80", "PRIME-PVCAM Version": "3.7.1", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Gain": "1-HDR", "640 Shutter-TriggerSequenceLength": "1000", "528 AO-SupportsTriggering": "0", "445 AO-TriggerInputLine": "/Dev1/PFI0", "OpticalPath-Name": "OpticalPath", "PRIME-PP 4 ENABLED": "Yes", "PRIME-PVCAM Adapter Version": "1.3.29", "COM3-Name": "COM3", "405 AO-MaxVolts": "5.0000", "520 Shutter-Block voltage": "false", "PRIME-Timing-ClearingTimeNs": "49344000.0000", "Core-Focus": "PIEZO", "528 AO-Block voltage": "false", "TE2000-Description": "Nikon TE2000 hub", "528 AO-MinVolts": "0.0000", "COM4-Parity": "None", "Summary": { "Slices": 11, "ChContrastMin": [ 0, 0, 0, 0, 0, 0 ], "PixelAspect": 1, "SlicesFirst": true, "Interval_ms": 0, "Time": "2019-12-19 09:59:21 -0500", "Prefix": "seq_13", "InitialPositionList": [ { "Label": "Pos0", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.21], "Focus": [17741.5], "XYStage": [ 44542.200000000004, 31636.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos1", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.22], "Focus": [17741.4], "XYStage": [ 43801.100000000006, 31637.9 ] }, "GridColumnIndex": 0 }, { "Label": "Pos2", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.5], "XYStage": [ 38561.3, 32040.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos3", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.185], "Focus": [17741.3], "XYStage": [ 37698.4, 32041.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos4", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.16], "Focus": [17741.5], "XYStage": [ 32375.4, 31758.9 ] }, "GridColumnIndex": 0 }, { "Label": "Pos5", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.17000000000002], "Focus": [17741.3], "XYStage": [ 31770.7, 31758.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos6", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.4], "XYStage": [ 26536.800000000003, 31068.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos7", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.205], "Focus": [17741.4], "XYStage": [ 25420.2, 31068.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos8", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.85], "Focus": [17741.3], "XYStage": [ 21059, 31508 ] }, "GridColumnIndex": 0 }, { "Label": "Pos9", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.82], "Focus": [17741.3], "XYStage": [ 19731.9, 31506.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos10", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.79], "Focus": [17741.4], "XYStage": [ 14528.5, 32007.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos11", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.83], "Focus": [17741.2], "XYStage": [ 13830.300000000001, 32009.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos12", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.79], "Focus": [17741.3], "XYStage": [ 8775.300000000001, 32017.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos13", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.815], "Focus": [17741.3], "XYStage": [ 8109.200000000001, 32008.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos14", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.805], "Focus": [17741.3], "XYStage": [ 3483.3, 31908.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos15", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.82], "Focus": [17741.2], "XYStage": [ 2686.8, 31903.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos16", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [211.48000000000002], "Focus": [17741.1], "XYStage": [ -1978.9, 31973.600000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos17", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [211.51500000000001], "Focus": [17741.1], "XYStage": [ -2957.8, 31982.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos18", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.195], "Focus": [17741.5], "XYStage": [ 47747.4, -576.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos19", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.23000000000002], "Focus": [17741.6], "XYStage": [ 47053.9, -576.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos20", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.205], "Focus": [17741.4], "XYStage": [ 41871.200000000004, -479.8 ] }, "GridColumnIndex": 0 }, { "Label": "Pos21", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.4], "XYStage": [ 40882.600000000006, -392 ] }, "GridColumnIndex": 0 }, { "Label": "Pos22", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.24], "Focus": [17741.4], "XYStage": [ 35905.6, -135.3 ] }, "GridColumnIndex": 0 }, { "Label": "Pos23", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.195], "Focus": [17741.5], "XYStage": [ 34990.9, -132.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos24", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.46], "Focus": [17741.4], "XYStage": [ 29678.100000000002, -958.1 ] }, "GridColumnIndex": 0 }, { "Label": "Pos25", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.44], "Focus": [17741.5], "XYStage": [ 28983.600000000002, -939.4000000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos26", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.475], "Focus": [17741.5], "XYStage": [ 23019.7, -122 ] }, "GridColumnIndex": 0 }, { "Label": "Pos27", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.44], "Focus": [17741.7], "XYStage": [ 22428, -116.80000000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos28", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.46], "Focus": [17741.7], "XYStage": [ 16364.900000000001, -181.10000000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos29", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.48000000000002], "Focus": [17741.5], "XYStage": [ 15712.1, -180 ] }, "GridColumnIndex": 0 }, { "Label": "Pos30", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.45000000000002], "Focus": [17741.7], "XYStage": [ 10346.1, 77.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos31", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.49], "Focus": [17741.4], "XYStage": [ 9755.5, 85.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos32", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.475], "Focus": [17741.5], "XYStage": [ 4775.5, 293.90000000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos33", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.445], "Focus": [17741.4], "XYStage": [ 3954.9, 293.90000000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos34", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.455], "Focus": [17741.4], "XYStage": [ -1324.9, 545.6 ] }, "GridColumnIndex": 0 }, { "Label": "Pos35", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.445], "Focus": [17741.3], "XYStage": [ -2097, 576.6 ] }, "GridColumnIndex": 0 } ], "z-step_um": 10, "GridRow": 0, "BitDepth": 16, "Depth": 2, "Channels": 6, "Source": "Micro-Manager", "ChColors": [ -16711681, -256, -65281, -1, -1, -16776961 ], "MetadataVersion": 10, "KeepShutterOpenChannels": true, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536, 65536 ], "CustomIntervals_ms": [], "Height": 1200, "MicroManagerVersion": "1.4.23 20180219", "GridColumn": 0, "UUID": "18808e7c-f2e6-47b3-90d8-1d779ce87dbd", "PixelType": "GRAY16", "Width": 1200, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\Yan\\20191218-YC113-S7S8-miseq", "Frames": 1, "Comment": "", "UserName": "Nikon", "PixelSize_um": 1.1, "ROI": [ 0, 0, 1200, 1200 ], "Date": "2019-12-19", "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC", "P-DAPI-high" ], "KeepShutterOpenSlices": true, "IJType": 1, "ComputerName": "ZadorConfocal2", "Positions": 36, "PositionIndex": 34, "CameraTimeout": 20000, "TimeFirst": false }, "640 AO-Name": "AnalogIO", "Vincent-D3-Command": "Close" }, "FrameKey-0-2-2": { "COM4-StopBits": "1", "528 AO-MaxVolts": "5.0000", "XLIGHT Excitation Wheel-State": "1", "470 Shutter-Name": "AnalogIO", "Core-Shutter": "640", "555 AO-Demo": "No", "XLIGHT Dichroic-State": "2", "XYStage-StepSize-Y": "0.1000", "ZPositionUm": 188.455, "XYStage-StepSize-X": "0.1000", "PRIME-FirmwareVersion": "20.28", "PRIME-ClearCycles": "2", "520-Name": "DA Shutter", "405 Shutter-Name": "AnalogIO", "PIEZO-StepSize": "0.0050", "PRIME-ClearMode": "Pre-Exposure", "470 AO-MinVolts": "0.0000", "405 AO-SupportsTriggering": "1", "CameraChannelIndex": 0, "XYStage-StartSpeed": "500.0000", "COM9-StopBits": "2", "405 AO-Description": "NI DAC", "COM4-DelayBetweenCharsMs": "0.0000", "445 AO-Block voltage": "false", "520 AO-TriggerInputLine": "/Dev1/PFI0", "555 AO-Description": "NI DAC", "520 AO-TriggerSampleRate": "100000", "COM4-Verbose": "1", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM4-Name": "COM4", "405 Shutter-Volts": "0.0000", "Vincent-D3-Port": "COM1", "PRIME-PP 4 MIN ADU AFFECTED": "200", "470 Shutter-MinVolts": "0.0000", "PRIME-PP 3 ENABLED": "Yes", "470 AO-Description": "NI DAC", "PRIME-Description": "PVCAM API device adapter", "520 Shutter-MaxVolts": "5.0000", "520 Shutter-Volts": "0.0000", "528 AO-Sequenceable": "1", "PRIME-Binning": "1x1", "555 Shutter-MaxVolts": "5.0000", "528 Shutter-TriggerSequenceLength": "1000", "405 Shutter-Block voltage": "false", "XLIGHT Spin motor-State": "1", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "Vincent-D3-Time to open (ms)": "35.0000", "Vincent-D3-Time to close (ms)": "35.0000", "445 AO-TriggerSampleRate": "100000", "640 Shutter-IOChannel": "Dev1/ao14", "555 AO-Sequenceable": "1", "520 AO-Name": "AnalogIO", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "470 AO-Block voltage": "false", "COM1-Verbose": "1", "COM9-AnswerTimeout": "10000.0000", "528 Shutter-Name": "AnalogIO", "PRIME-Trigger Timeout (secs)": "10", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "NextFrame": 0, "470 AO-TriggerSequenceLength": "1000", "PRIME-TransposeXY": "0", "445 AO-TriggerSequenceLength": "1000", "COM1-Name": "COM1", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "COM9-DataBits": "8", "COM1-AnswerTimeout": "500.0000", "Camera": "", "TE2000-Version": "V2.50-V2.50-V2.10", "528 Shutter-Volts": "0.0000", "XLIGHT Hub-Name": "XLIGHT Hub", "PIEZO-Description": "Ludl stage driver adapter", "PositionName": "Pos34", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSampleRate": "100000", "PRIME-FanSpeedSetpoint": "High", "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "405 AO-TriggerSampleRate": "100000", "470 Shutter-Block voltage": "false", "470-State": "0", "COM3-Parity": "None", "405 AO-Volts": "0.0000", "PRIME-TransposeMirrorY": "0", "PRIME-ChipName": "GS144BSI", "470 AO-Volts": "0.0000", "PRIME-TransposeMirrorX": "0", "445 Shutter-SupportsTriggering": "0", "470 Shutter-SupportsTriggering": "0", "XLIGHT Hub-Description": "XLIGHT hub", "PRIME-PreampOffLimit": "10000", "470 AO-Sequenceable": "1", "528 Shutter-MaxVolts": "5.0000", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "Core-XYStage": "XYStage", "470 AO-TriggerSampleRate": "100000", "PRIME-Timing-PreTriggerDelayNs": "24672000.0000", "528-DA Device": "528 Shutter", "Focus-Description": "Nikon TE2000 focus adapter", "FilterBlock-State": "5", "520 AO-Demo": "No", "COM3-DelayBetweenCharsMs": "0.0000", "470 Shutter-Volts": "0.0000", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "PRIME-PP 2 THRESHOLD": "125", "PRIME-PP 4 THRESHOLD": "75", "XYStage-Acceleration": "4.0000", "470 Shutter-Demo": "No", "PRIME-Name": "PMPCIECam00", "640-DA Device": "640 Shutter", "405 AO-Block voltage": "false", "ElapsedTime-ms": 1404517, "PRIME-Offset": "100", "445 AO-IOChannel": "Dev1/ao1", "640 AO-MaxVolts": "5.0000", "PositionIndex": 34, "PRIME-PP 1 ENABLED": "Yes", "COM1-BaudRate": "9600", "640-State": "1", "COM3-Verbose": "1", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "LudlController-TransmissionDelay": "0", "PRIME-FullWellCapacity": "62000", "PRIME-SMARTStreamingEnabled": "No", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "640 Shutter-Description": "NI DAC", "528-Description": "DA device that is used as a shutter", "COM4-Description": "Serial port driver (boost:asio)", "405 Shutter-Description": "NI DAC", "TE2000-Name": "TE2000", "528 Shutter-Description": "NI DAC", "COM9-Description": "Serial port driver (boost:asio)", "LudlController-Name": "LudlController", "445 Shutter-TriggerSequenceLength": "1000", "PRIME-SerialNumber": "A18B203008", "445 AO-Volts": "0.0000", "PRIME-X-dimension": "1200", "PRIME-ShutterCloseDelay": "0", "XLIGHT Disk slider-State": "2", "Position": "Default", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 AO-IOChannel": "Dev1/ao5", "520 AO-IOChannel": "Dev1/ao4", "528 AO-TriggerSequenceLength": "1000", "405 Shutter-MaxVolts": "5.0000", "470-DA Device": "470 Shutter", "PRIME-FTCapable": "No", "Core-ChannelGroup": "Channels", "555 AO-SupportsTriggering": "0", "PRIME-Y-dimension": "1200", "Frame": 0, "555 Shutter-Sequenceable": "1", "PRIME-Timing-ExposureTimeNs": "499998000.0000", "Core-ImageProcessor": "", "528-State": "0", "520 AO-Block voltage": "false", "XLIGHT Excitation Wheel-Label": "QUAD", "640 Shutter-Demo": "No", "COM3-StopBits": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "520 Shutter-IOChannel": "Dev1/ao11", "Width": 1200, "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-SupportsTriggering": "0", "640 AO-TriggerSampleRate": "100000", "520-Description": "DA device that is used as a shutter", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "405 Shutter-Sequenceable": "1", "405 Shutter-SupportsTriggering": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-TriggerSampleRate": "100000", "640 Shutter-MaxVolts": "5.0000", "555 AO-MaxVolts": "5.0000", "520 AO-TriggerSequenceLength": "1000", "COM3-BaudRate": "9600", "COM9-BaudRate": "9600", "Vincent-D3-Channel #": "Ch. #1", "PRIME-PMode": "Normal", "OpticalPath-Label": "Left(100)", "BitDepth": 16, "555 AO-TriggerSequenceLength": "1000", "445 Shutter-Description": "NI DAC", "405 AO-Sequenceable": "1", "PRIME-AcquisitionMethod": "Callbacks", "555 AO-Name": "AnalogIO", "PRIME-ShutterMode": "Pre-Exposure", "COM3-Handshaking": "Off", "445 AO-SupportsTriggering": "0", "ROI": "0-0-1200-1200", "COM9-Verbose": "1", "PRIME-Color": "OFF", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "PRIME-ShutterOpenDelay": "0", "COM1-Parity": "None", "Slice": 2, "SlicePosition": -30, "COM9-DelayBetweenCharsMs": "0.0000", "555-Name": "DA Shutter", "PIEZO-LudlSingleAxisName": "B", "XLIGHT Emission Wheel-Label": "679/29 (A)", "640 Shutter-Name": "AnalogIO", "640 AO-Sequenceable": "1", "520 Shutter-Demo": "No", "528 Shutter-Demo": "No", "470-Name": "DA Shutter", "520-DA Device": "520 Shutter", "XYStage-Description": "Ludl XY stage driver adapter", "445 Shutter-MinVolts": "0.0000", "528 AO-TriggerSampleRate": "100000", "405 AO-MinVolts": "0.0000", "405 Shutter-TriggerSequenceLength": "1000", "Height": 1200, "555 AO-Block voltage": "false", "640 Shutter-Volts": "5.0000", "PixelType": "GRAY16", "445 Shutter-Block voltage": "false", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "520 Shutter-TriggerSequenceLength": "1000", "COM9-Name": "COM9", "Core-SLM": "", "555 AO-Volts": "0.0000", "555 Shutter-Block voltage": "false", "XYStage-Speed": "1800.0000", "520 AO-MaxVolts": "5.0000", "528 Shutter-SupportsTriggering": "0", "COM1-Description": "Serial port driver (boost:asio)", "PRIME-MetadataEnabled": "No", "520 Shutter-MinVolts": "0.0000", "470 Shutter-Sequenceable": "1", "PRIME-PreampDelay": "0", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-MinVolts": "0.0000", "ChannelIndex": 2, "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Core-AutoShutter": "1", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "Exposure-ms": 320, "PRIME-PP 2 ENABLED": "Yes", "640 Shutter-TriggerSampleRate": "100000", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "470 Shutter-IOChannel": "Dev1/ao10", "405 AO-Demo": "No", "PixelSizeUm": 1.1, "470 AO-Demo": "No", "470 Shutter-MaxVolts": "5.0000", "Vincent-D3-Name": "Vincent-D3", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FileName": "img_000000000_Il-A_002.tif", "LudlController-Reset": "Operate", "PRIME-Exposure": "320.00", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "445-Name": "DA Shutter", "555 Shutter-Name": "AnalogIO", "520 Shutter-TriggerSampleRate": "100000", "445 Shutter-IOChannel": "Dev1/ao9", "PRIME-TransposeCorrection": "0", "XYStage-StepSize": "0.1000", "445-Description": "DA device that is used as a shutter", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 AO-IOChannel": "Dev1/ao7", "520 Shutter-Name": "AnalogIO", "COM3-DataBits": "8", "640 AO-Volts": "4.5000", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "555 Shutter-IOChannel": "Dev1/ao13", "WaitInterval": null, "520 Shutter-Sequenceable": "1", "YPositionUm": 545.6, "445-State": "0", "445-DA Device": "445 Shutter", "PRIME-CircularBufferAutoSize": "ON", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "555 AO-TriggerSampleRate": "100000", "445 Shutter-Volts": "0.0000", "XLIGHT Hub-Port": "COM3", "Core-AutoFocus": "", "COM4-Handshaking": "Off", "528 Shutter-MinVolts": "0.0000", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "445 Shutter-Name": "AnalogIO", "528 Shutter-Sequenceable": "1", "XYStage-TransposeMirrorX": "0", "XYStage-TransposeMirrorY": "0", "PRIME-ReadoutRate": "100MHz 16bit", "XYStage-Name": "Stage", "640 AO-SupportsTriggering": "0", "640-Name": "DA Shutter", "640 Shutter-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "640 AO-Block voltage": "false", "445 Shutter-Sequenceable": "1", "470 AO-MaxVolts": "5.0000", "COM4-BaudRate": "9600", "470-Description": "DA device that is used as a shutter", "520 Shutter-Description": "NI DAC", "PRIME-CCDTemperature": "-14.8500", "Time": "2019-12-19 09:59:34 -0500", "640 AO-TriggerSequenceLength": "1000", "470 AO-Name": "AnalogIO", "XLIGHT Disk slider-Label": "Disk Pos 40um", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "Binning": "1x1", "TE2000-ModelType": "1", "COM3-AnswerTimeout": "500.0000", "555 Shutter-SupportsTriggering": "0", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "TE2000-Port": "COM4", "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Description": "NI DAC", "COM4-AnswerTimeout": "500.0000", "COM1-DelayBetweenCharsMs": "0.0000", "FilterBlock-Label": "Confocal", "445 AO-Name": "AnalogIO", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-Timing-ReadoutTimeNs": "24672000.0000", "528 AO-Demo": "No", "520 AO-Sequenceable": "1", "555-Description": "DA device that is used as a shutter", "405-DA Device": "405 Shutter", "405 AO-TriggerSequenceLength": "1000", "640 AO-MinVolts": "0.0000", "640 Shutter-Block voltage": "false", "FrameIndex": 0, "PRIME-Port": "CMOS", "555 AO-MinVolts": "0.0000", "COM3-Description": "Serial port driver (boost:asio)", "528 AO-Description": "NI DAC", "555 AO-IOChannel": "Dev1/ao6", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "555 AO-TriggerInputLine": "/Dev1/PFI0", "PRIME-ActualInterval-ms": "390.5200", "LudlController-Port": "COM9", "445 Shutter-Demo": "No", "PRIME-Trigger-Expose Out-Mux": "1", "SliceIndex": 2, "PRIME-CCDTemperatureSetPoint": "-15.0000", "405 Shutter-MinVolts": "0.0000", "PRIME-ExposeOutMode": "First Row", "AxisPositions": null, "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Volts": "0.0000", "528-Name": "DA Shutter", "555-State": "0", "XLIGHT Dichroic-Label": "Di03-R635-t1", "445 AO-Sequenceable": "1", "405-Name": "DA Shutter", "405 AO-IOChannel": "Dev1/ao0", "Core-Camera": "PRIME", "Focus-Name": "Focus", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "405 Shutter-IOChannel": "Dev1/ao8", "640 AO-Demo": "No", "640 Shutter-SupportsTriggering": "0", "445 AO-Demo": "No", "555-DA Device": "555 Shutter", "470 Shutter-Description": "NI DAC", "528 AO-Volts": "0.0000", "COM1-DataBits": "8", "Vincent-D3-Address": "x", "520 AO-SupportsTriggering": "0", "405-Description": "DA device that is used as a shutter", "640 Shutter-MinVolts": "0.0000", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "405 AO-Name": "AnalogIO", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "640 AO-TriggerInputLine": "/Dev1/PFI0", "405 AO-TriggerInputLine": "/Dev1/PFI0", "640-Description": "DA device that is used as a shutter", "445 Shutter-TriggerSampleRate": "100000", "470 Shutter-TriggerSequenceLength": "1000", "COM1-StopBits": "1", "520 Shutter-SupportsTriggering": "0", "405-State": "0", "Core-Initialize": "1", "XLIGHT Emission Wheel-State": "3", "520 AO-Description": "NI DAC", "528 AO-TriggerInputLine": "/Dev1/PFI0", "445 Shutter-MaxVolts": "5.0000", "COM9-Handshaking": "Off", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "XLIGHT Spin motor-Label": "On (spinning)", "PRIME-PP 5 ENABLED": "No", "UUID": "adf028a1-1f52-4665-8795-b2a8975c2ce3", "PRIME-TriggerMode": "Internal Trigger", "COM1-Handshaking": "Off", "PRIME-Timing-PostTriggerDelayNs": "49344000.0000", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "Focus-Position": "17741.5000", "528 Shutter-Block voltage": "false", "Channel": "Il-A", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "555 Shutter-Demo": "No", "405 Shutter-Demo": "No", "528 AO-Name": "AnalogIO", "520-State": "0", "Core-Galvo": "", "405 Shutter-TriggerSampleRate": "100000", "Focus-FocusStepSize_(nm)": "100", "PRIME-PixelType": "16bit", "COM4-DataBits": "8", "470 AO-IOChannel": "Dev1/ao2", "520 AO-MinVolts": "0.0000", "COM9-Parity": "None", "PRIME-CircularBufferEnabled": "ON", "PRIME-PP 6 Reset": "No", "640 AO-Description": "NI DAC", "Source": "PRIME", "445 AO-MinVolts": "0.0000", "PIEZO-Name": "Stage", "445 AO-Description": "NI DAC", "PRIME-CircularBufferFrameCount": "46", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "520 AO-Volts": "0.0000", "528 Shutter-TriggerSampleRate": "100000", "XPositionUm": -1324.9, "OpticalPath-State": "4", "PRIME-PP 3 THRESHOLD": "80", "PRIME-PVCAM Version": "3.7.1", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Gain": "1-HDR", "640 Shutter-TriggerSequenceLength": "1000", "528 AO-SupportsTriggering": "0", "445 AO-TriggerInputLine": "/Dev1/PFI0", "OpticalPath-Name": "OpticalPath", "PRIME-PP 4 ENABLED": "Yes", "PRIME-PVCAM Adapter Version": "1.3.29", "COM3-Name": "COM3", "405 AO-MaxVolts": "5.0000", "520 Shutter-Block voltage": "false", "PRIME-Timing-ClearingTimeNs": "49344000.0000", "Core-Focus": "PIEZO", "528 AO-Block voltage": "false", "TE2000-Description": "Nikon TE2000 hub", "528 AO-MinVolts": "0.0000", "COM4-Parity": "None", "Summary": { "Slices": 11, "ChContrastMin": [ 0, 0, 0, 0, 0, 0 ], "PixelAspect": 1, "SlicesFirst": true, "Interval_ms": 0, "Time": "2019-12-19 09:59:21 -0500", "Prefix": "seq_13", "InitialPositionList": [ { "Label": "Pos0", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.21], "Focus": [17741.5], "XYStage": [ 44542.200000000004, 31636.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos1", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.22], "Focus": [17741.4], "XYStage": [ 43801.100000000006, 31637.9 ] }, "GridColumnIndex": 0 }, { "Label": "Pos2", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.5], "XYStage": [ 38561.3, 32040.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos3", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.185], "Focus": [17741.3], "XYStage": [ 37698.4, 32041.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos4", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.16], "Focus": [17741.5], "XYStage": [ 32375.4, 31758.9 ] }, "GridColumnIndex": 0 }, { "Label": "Pos5", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.17000000000002], "Focus": [17741.3], "XYStage": [ 31770.7, 31758.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos6", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.4], "XYStage": [ 26536.800000000003, 31068.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos7", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.205], "Focus": [17741.4], "XYStage": [ 25420.2, 31068.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos8", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.85], "Focus": [17741.3], "XYStage": [ 21059, 31508 ] }, "GridColumnIndex": 0 }, { "Label": "Pos9", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.82], "Focus": [17741.3], "XYStage": [ 19731.9, 31506.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos10", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.79], "Focus": [17741.4], "XYStage": [ 14528.5, 32007.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos11", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.83], "Focus": [17741.2], "XYStage": [ 13830.300000000001, 32009.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos12", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.79], "Focus": [17741.3], "XYStage": [ 8775.300000000001, 32017.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos13", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.815], "Focus": [17741.3], "XYStage": [ 8109.200000000001, 32008.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos14", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.805], "Focus": [17741.3], "XYStage": [ 3483.3, 31908.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos15", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.82], "Focus": [17741.2], "XYStage": [ 2686.8, 31903.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos16", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [211.48000000000002], "Focus": [17741.1], "XYStage": [ -1978.9, 31973.600000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos17", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [211.51500000000001], "Focus": [17741.1], "XYStage": [ -2957.8, 31982.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos18", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.195], "Focus": [17741.5], "XYStage": [ 47747.4, -576.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos19", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.23000000000002], "Focus": [17741.6], "XYStage": [ 47053.9, -576.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos20", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.205], "Focus": [17741.4], "XYStage": [ 41871.200000000004, -479.8 ] }, "GridColumnIndex": 0 }, { "Label": "Pos21", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.4], "XYStage": [ 40882.600000000006, -392 ] }, "GridColumnIndex": 0 }, { "Label": "Pos22", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.24], "Focus": [17741.4], "XYStage": [ 35905.6, -135.3 ] }, "GridColumnIndex": 0 }, { "Label": "Pos23", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.195], "Focus": [17741.5], "XYStage": [ 34990.9, -132.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos24", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.46], "Focus": [17741.4], "XYStage": [ 29678.100000000002, -958.1 ] }, "GridColumnIndex": 0 }, { "Label": "Pos25", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.44], "Focus": [17741.5], "XYStage": [ 28983.600000000002, -939.4000000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos26", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.475], "Focus": [17741.5], "XYStage": [ 23019.7, -122 ] }, "GridColumnIndex": 0 }, { "Label": "Pos27", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.44], "Focus": [17741.7], "XYStage": [ 22428, -116.80000000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos28", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.46], "Focus": [17741.7], "XYStage": [ 16364.900000000001, -181.10000000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos29", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.48000000000002], "Focus": [17741.5], "XYStage": [ 15712.1, -180 ] }, "GridColumnIndex": 0 }, { "Label": "Pos30", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.45000000000002], "Focus": [17741.7], "XYStage": [ 10346.1, 77.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos31", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.49], "Focus": [17741.4], "XYStage": [ 9755.5, 85.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos32", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.475], "Focus": [17741.5], "XYStage": [ 4775.5, 293.90000000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos33", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.445], "Focus": [17741.4], "XYStage": [ 3954.9, 293.90000000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos34", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.455], "Focus": [17741.4], "XYStage": [ -1324.9, 545.6 ] }, "GridColumnIndex": 0 }, { "Label": "Pos35", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.445], "Focus": [17741.3], "XYStage": [ -2097, 576.6 ] }, "GridColumnIndex": 0 } ], "z-step_um": 10, "GridRow": 0, "BitDepth": 16, "Depth": 2, "Channels": 6, "Source": "Micro-Manager", "ChColors": [ -16711681, -256, -65281, -1, -1, -16776961 ], "MetadataVersion": 10, "KeepShutterOpenChannels": true, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536, 65536 ], "CustomIntervals_ms": [], "Height": 1200, "MicroManagerVersion": "1.4.23 20180219", "GridColumn": 0, "UUID": "18808e7c-f2e6-47b3-90d8-1d779ce87dbd", "PixelType": "GRAY16", "Width": 1200, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\Yan\\20191218-YC113-S7S8-miseq", "Frames": 1, "Comment": "", "UserName": "Nikon", "PixelSize_um": 1.1, "ROI": [ 0, 0, 1200, 1200 ], "Date": "2019-12-19", "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC", "P-DAPI-high" ], "KeepShutterOpenSlices": true, "IJType": 1, "ComputerName": "ZadorConfocal2", "Positions": 36, "PositionIndex": 34, "CameraTimeout": 20000, "TimeFirst": false }, "640 AO-Name": "AnalogIO", "Vincent-D3-Command": "Close" }, "FrameKey-0-2-3": { "COM4-StopBits": "1", "528 AO-MaxVolts": "5.0000", "XLIGHT Excitation Wheel-State": "1", "470 Shutter-Name": "AnalogIO", "Core-Shutter": "640", "555 AO-Demo": "No", "XLIGHT Dichroic-State": "2", "XYStage-StepSize-Y": "0.1000", "ZPositionUm": 198.455, "XYStage-StepSize-X": "0.1000", "PRIME-FirmwareVersion": "20.28", "PRIME-ClearCycles": "2", "520-Name": "DA Shutter", "405 Shutter-Name": "AnalogIO", "PIEZO-StepSize": "0.0050", "PRIME-ClearMode": "Pre-Exposure", "470 AO-MinVolts": "0.0000", "405 AO-SupportsTriggering": "1", "CameraChannelIndex": 0, "XYStage-StartSpeed": "500.0000", "COM9-StopBits": "2", "405 AO-Description": "NI DAC", "COM4-DelayBetweenCharsMs": "0.0000", "445 AO-Block voltage": "false", "520 AO-TriggerInputLine": "/Dev1/PFI0", "555 AO-Description": "NI DAC", "520 AO-TriggerSampleRate": "100000", "COM4-Verbose": "1", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM4-Name": "COM4", "405 Shutter-Volts": "0.0000", "Vincent-D3-Port": "COM1", "PRIME-PP 4 MIN ADU AFFECTED": "200", "470 Shutter-MinVolts": "0.0000", "PRIME-PP 3 ENABLED": "Yes", "470 AO-Description": "NI DAC", "PRIME-Description": "PVCAM API device adapter", "520 Shutter-MaxVolts": "5.0000", "520 Shutter-Volts": "0.0000", "528 AO-Sequenceable": "1", "PRIME-Binning": "1x1", "555 Shutter-MaxVolts": "5.0000", "528 Shutter-TriggerSequenceLength": "1000", "405 Shutter-Block voltage": "false", "XLIGHT Spin motor-State": "1", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "Vincent-D3-Time to open (ms)": "35.0000", "Vincent-D3-Time to close (ms)": "35.0000", "445 AO-TriggerSampleRate": "100000", "640 Shutter-IOChannel": "Dev1/ao14", "555 AO-Sequenceable": "1", "520 AO-Name": "AnalogIO", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "470 AO-Block voltage": "false", "COM1-Verbose": "1", "COM9-AnswerTimeout": "10000.0000", "528 Shutter-Name": "AnalogIO", "PRIME-Trigger Timeout (secs)": "10", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "NextFrame": 0, "470 AO-TriggerSequenceLength": "1000", "PRIME-TransposeXY": "0", "445 AO-TriggerSequenceLength": "1000", "COM1-Name": "COM1", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "COM9-DataBits": "8", "COM1-AnswerTimeout": "500.0000", "Camera": "", "TE2000-Version": "V2.50-V2.50-V2.10", "528 Shutter-Volts": "0.0000", "XLIGHT Hub-Name": "XLIGHT Hub", "PIEZO-Description": "Ludl stage driver adapter", "PositionName": "Pos34", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSampleRate": "100000", "PRIME-FanSpeedSetpoint": "High", "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "405 AO-TriggerSampleRate": "100000", "470 Shutter-Block voltage": "false", "470-State": "0", "COM3-Parity": "None", "405 AO-Volts": "0.0000", "PRIME-TransposeMirrorY": "0", "PRIME-ChipName": "GS144BSI", "470 AO-Volts": "0.0000", "PRIME-TransposeMirrorX": "0", "445 Shutter-SupportsTriggering": "0", "470 Shutter-SupportsTriggering": "0", "XLIGHT Hub-Description": "XLIGHT hub", "PRIME-PreampOffLimit": "10000", "470 AO-Sequenceable": "1", "528 Shutter-MaxVolts": "5.0000", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "Core-XYStage": "XYStage", "470 AO-TriggerSampleRate": "100000", "PRIME-Timing-PreTriggerDelayNs": "24672000.0000", "528-DA Device": "528 Shutter", "Focus-Description": "Nikon TE2000 focus adapter", "FilterBlock-State": "5", "520 AO-Demo": "No", "COM3-DelayBetweenCharsMs": "0.0000", "470 Shutter-Volts": "0.0000", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "PRIME-PP 2 THRESHOLD": "125", "PRIME-PP 4 THRESHOLD": "75", "XYStage-Acceleration": "4.0000", "470 Shutter-Demo": "No", "PRIME-Name": "PMPCIECam00", "640-DA Device": "640 Shutter", "405 AO-Block voltage": "false", "ElapsedTime-ms": 1405074, "PRIME-Offset": "100", "445 AO-IOChannel": "Dev1/ao1", "640 AO-MaxVolts": "5.0000", "PositionIndex": 34, "PRIME-PP 1 ENABLED": "Yes", "COM1-BaudRate": "9600", "640-State": "1", "COM3-Verbose": "1", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "LudlController-TransmissionDelay": "0", "PRIME-FullWellCapacity": "62000", "PRIME-SMARTStreamingEnabled": "No", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "640 Shutter-Description": "NI DAC", "528-Description": "DA device that is used as a shutter", "COM4-Description": "Serial port driver (boost:asio)", "405 Shutter-Description": "NI DAC", "TE2000-Name": "TE2000", "528 Shutter-Description": "NI DAC", "COM9-Description": "Serial port driver (boost:asio)", "LudlController-Name": "LudlController", "445 Shutter-TriggerSequenceLength": "1000", "PRIME-SerialNumber": "A18B203008", "445 AO-Volts": "0.0000", "PRIME-X-dimension": "1200", "PRIME-ShutterCloseDelay": "0", "XLIGHT Disk slider-State": "2", "Position": "Default", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 AO-IOChannel": "Dev1/ao5", "520 AO-IOChannel": "Dev1/ao4", "528 AO-TriggerSequenceLength": "1000", "405 Shutter-MaxVolts": "5.0000", "470-DA Device": "470 Shutter", "PRIME-FTCapable": "No", "Core-ChannelGroup": "Channels", "555 AO-SupportsTriggering": "0", "PRIME-Y-dimension": "1200", "Frame": 0, "555 Shutter-Sequenceable": "1", "PRIME-Timing-ExposureTimeNs": "499998000.0000", "Core-ImageProcessor": "", "528-State": "0", "520 AO-Block voltage": "false", "XLIGHT Excitation Wheel-Label": "QUAD", "640 Shutter-Demo": "No", "COM3-StopBits": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "520 Shutter-IOChannel": "Dev1/ao11", "Width": 1200, "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-SupportsTriggering": "0", "640 AO-TriggerSampleRate": "100000", "520-Description": "DA device that is used as a shutter", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "405 Shutter-Sequenceable": "1", "405 Shutter-SupportsTriggering": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-TriggerSampleRate": "100000", "640 Shutter-MaxVolts": "5.0000", "555 AO-MaxVolts": "5.0000", "520 AO-TriggerSequenceLength": "1000", "COM3-BaudRate": "9600", "COM9-BaudRate": "9600", "Vincent-D3-Channel #": "Ch. #1", "PRIME-PMode": "Normal", "OpticalPath-Label": "Left(100)", "BitDepth": 16, "555 AO-TriggerSequenceLength": "1000", "445 Shutter-Description": "NI DAC", "405 AO-Sequenceable": "1", "PRIME-AcquisitionMethod": "Callbacks", "555 AO-Name": "AnalogIO", "PRIME-ShutterMode": "Pre-Exposure", "COM3-Handshaking": "Off", "445 AO-SupportsTriggering": "0", "ROI": "0-0-1200-1200", "COM9-Verbose": "1", "PRIME-Color": "OFF", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "PRIME-ShutterOpenDelay": "0", "COM1-Parity": "None", "Slice": 3, "SlicePosition": -20, "COM9-DelayBetweenCharsMs": "0.0000", "555-Name": "DA Shutter", "PIEZO-LudlSingleAxisName": "B", "XLIGHT Emission Wheel-Label": "679/29 (A)", "640 Shutter-Name": "AnalogIO", "640 AO-Sequenceable": "1", "520 Shutter-Demo": "No", "528 Shutter-Demo": "No", "470-Name": "DA Shutter", "520-DA Device": "520 Shutter", "XYStage-Description": "Ludl XY stage driver adapter", "445 Shutter-MinVolts": "0.0000", "528 AO-TriggerSampleRate": "100000", "405 AO-MinVolts": "0.0000", "405 Shutter-TriggerSequenceLength": "1000", "Height": 1200, "555 AO-Block voltage": "false", "640 Shutter-Volts": "5.0000", "PixelType": "GRAY16", "445 Shutter-Block voltage": "false", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "520 Shutter-TriggerSequenceLength": "1000", "COM9-Name": "COM9", "Core-SLM": "", "555 AO-Volts": "0.0000", "555 Shutter-Block voltage": "false", "XYStage-Speed": "1800.0000", "520 AO-MaxVolts": "5.0000", "528 Shutter-SupportsTriggering": "0", "COM1-Description": "Serial port driver (boost:asio)", "PRIME-MetadataEnabled": "No", "520 Shutter-MinVolts": "0.0000", "470 Shutter-Sequenceable": "1", "PRIME-PreampDelay": "0", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-MinVolts": "0.0000", "ChannelIndex": 2, "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Core-AutoShutter": "1", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "Exposure-ms": 320, "PRIME-PP 2 ENABLED": "Yes", "640 Shutter-TriggerSampleRate": "100000", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "470 Shutter-IOChannel": "Dev1/ao10", "405 AO-Demo": "No", "PixelSizeUm": 1.1, "470 AO-Demo": "No", "470 Shutter-MaxVolts": "5.0000", "Vincent-D3-Name": "Vincent-D3", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FileName": "img_000000000_Il-A_003.tif", "LudlController-Reset": "Operate", "PRIME-Exposure": "320.00", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "445-Name": "DA Shutter", "555 Shutter-Name": "AnalogIO", "520 Shutter-TriggerSampleRate": "100000", "445 Shutter-IOChannel": "Dev1/ao9", "PRIME-TransposeCorrection": "0", "XYStage-StepSize": "0.1000", "445-Description": "DA device that is used as a shutter", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 AO-IOChannel": "Dev1/ao7", "520 Shutter-Name": "AnalogIO", "COM3-DataBits": "8", "640 AO-Volts": "4.5000", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "555 Shutter-IOChannel": "Dev1/ao13", "WaitInterval": null, "520 Shutter-Sequenceable": "1", "YPositionUm": 545.6, "445-State": "0", "445-DA Device": "445 Shutter", "PRIME-CircularBufferAutoSize": "ON", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "555 AO-TriggerSampleRate": "100000", "445 Shutter-Volts": "0.0000", "XLIGHT Hub-Port": "COM3", "Core-AutoFocus": "", "COM4-Handshaking": "Off", "528 Shutter-MinVolts": "0.0000", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "445 Shutter-Name": "AnalogIO", "528 Shutter-Sequenceable": "1", "XYStage-TransposeMirrorX": "0", "XYStage-TransposeMirrorY": "0", "PRIME-ReadoutRate": "100MHz 16bit", "XYStage-Name": "Stage", "640 AO-SupportsTriggering": "0", "640-Name": "DA Shutter", "640 Shutter-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "640 AO-Block voltage": "false", "445 Shutter-Sequenceable": "1", "470 AO-MaxVolts": "5.0000", "COM4-BaudRate": "9600", "470-Description": "DA device that is used as a shutter", "520 Shutter-Description": "NI DAC", "PRIME-CCDTemperature": "-14.8500", "Time": "2019-12-19 09:59:35 -0500", "640 AO-TriggerSequenceLength": "1000", "470 AO-Name": "AnalogIO", "XLIGHT Disk slider-Label": "Disk Pos 40um", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "Binning": "1x1", "TE2000-ModelType": "1", "COM3-AnswerTimeout": "500.0000", "555 Shutter-SupportsTriggering": "0", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "TE2000-Port": "COM4", "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Description": "NI DAC", "COM4-AnswerTimeout": "500.0000", "COM1-DelayBetweenCharsMs": "0.0000", "FilterBlock-Label": "Confocal", "445 AO-Name": "AnalogIO", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-Timing-ReadoutTimeNs": "24672000.0000", "528 AO-Demo": "No", "520 AO-Sequenceable": "1", "555-Description": "DA device that is used as a shutter", "405-DA Device": "405 Shutter", "405 AO-TriggerSequenceLength": "1000", "640 AO-MinVolts": "0.0000", "640 Shutter-Block voltage": "false", "FrameIndex": 0, "PRIME-Port": "CMOS", "555 AO-MinVolts": "0.0000", "COM3-Description": "Serial port driver (boost:asio)", "528 AO-Description": "NI DAC", "555 AO-IOChannel": "Dev1/ao6", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "555 AO-TriggerInputLine": "/Dev1/PFI0", "PRIME-ActualInterval-ms": "390.5200", "LudlController-Port": "COM9", "445 Shutter-Demo": "No", "PRIME-Trigger-Expose Out-Mux": "1", "SliceIndex": 3, "PRIME-CCDTemperatureSetPoint": "-15.0000", "405 Shutter-MinVolts": "0.0000", "PRIME-ExposeOutMode": "First Row", "AxisPositions": null, "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Volts": "0.0000", "528-Name": "DA Shutter", "555-State": "0", "XLIGHT Dichroic-Label": "Di03-R635-t1", "445 AO-Sequenceable": "1", "405-Name": "DA Shutter", "405 AO-IOChannel": "Dev1/ao0", "Core-Camera": "PRIME", "Focus-Name": "Focus", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "405 Shutter-IOChannel": "Dev1/ao8", "640 AO-Demo": "No", "640 Shutter-SupportsTriggering": "0", "445 AO-Demo": "No", "555-DA Device": "555 Shutter", "470 Shutter-Description": "NI DAC", "528 AO-Volts": "0.0000", "COM1-DataBits": "8", "Vincent-D3-Address": "x", "520 AO-SupportsTriggering": "0", "405-Description": "DA device that is used as a shutter", "640 Shutter-MinVolts": "0.0000", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "405 AO-Name": "AnalogIO", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "640 AO-TriggerInputLine": "/Dev1/PFI0", "405 AO-TriggerInputLine": "/Dev1/PFI0", "640-Description": "DA device that is used as a shutter", "445 Shutter-TriggerSampleRate": "100000", "470 Shutter-TriggerSequenceLength": "1000", "COM1-StopBits": "1", "520 Shutter-SupportsTriggering": "0", "405-State": "0", "Core-Initialize": "1", "XLIGHT Emission Wheel-State": "3", "520 AO-Description": "NI DAC", "528 AO-TriggerInputLine": "/Dev1/PFI0", "445 Shutter-MaxVolts": "5.0000", "COM9-Handshaking": "Off", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "XLIGHT Spin motor-Label": "On (spinning)", "PRIME-PP 5 ENABLED": "No", "UUID": "f7611a3a-bc2f-4112-b0d1-350a2b488173", "PRIME-TriggerMode": "Internal Trigger", "COM1-Handshaking": "Off", "PRIME-Timing-PostTriggerDelayNs": "49344000.0000", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "Focus-Position": "17741.5000", "528 Shutter-Block voltage": "false", "Channel": "Il-A", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "555 Shutter-Demo": "No", "405 Shutter-Demo": "No", "528 AO-Name": "AnalogIO", "520-State": "0", "Core-Galvo": "", "405 Shutter-TriggerSampleRate": "100000", "Focus-FocusStepSize_(nm)": "100", "PRIME-PixelType": "16bit", "COM4-DataBits": "8", "470 AO-IOChannel": "Dev1/ao2", "520 AO-MinVolts": "0.0000", "COM9-Parity": "None", "PRIME-CircularBufferEnabled": "ON", "PRIME-PP 6 Reset": "No", "640 AO-Description": "NI DAC", "Source": "PRIME", "445 AO-MinVolts": "0.0000", "PIEZO-Name": "Stage", "445 AO-Description": "NI DAC", "PRIME-CircularBufferFrameCount": "46", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "520 AO-Volts": "0.0000", "528 Shutter-TriggerSampleRate": "100000", "XPositionUm": -1324.9, "OpticalPath-State": "4", "PRIME-PP 3 THRESHOLD": "80", "PRIME-PVCAM Version": "3.7.1", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Gain": "1-HDR", "640 Shutter-TriggerSequenceLength": "1000", "528 AO-SupportsTriggering": "0", "445 AO-TriggerInputLine": "/Dev1/PFI0", "OpticalPath-Name": "OpticalPath", "PRIME-PP 4 ENABLED": "Yes", "PRIME-PVCAM Adapter Version": "1.3.29", "COM3-Name": "COM3", "405 AO-MaxVolts": "5.0000", "520 Shutter-Block voltage": "false", "PRIME-Timing-ClearingTimeNs": "49344000.0000", "Core-Focus": "PIEZO", "528 AO-Block voltage": "false", "TE2000-Description": "Nikon TE2000 hub", "528 AO-MinVolts": "0.0000", "COM4-Parity": "None", "Summary": { "Slices": 11, "ChContrastMin": [ 0, 0, 0, 0, 0, 0 ], "PixelAspect": 1, "SlicesFirst": true, "Interval_ms": 0, "Time": "2019-12-19 09:59:21 -0500", "Prefix": "seq_13", "InitialPositionList": [ { "Label": "Pos0", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.21], "Focus": [17741.5], "XYStage": [ 44542.200000000004, 31636.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos1", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.22], "Focus": [17741.4], "XYStage": [ 43801.100000000006, 31637.9 ] }, "GridColumnIndex": 0 }, { "Label": "Pos2", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.5], "XYStage": [ 38561.3, 32040.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos3", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.185], "Focus": [17741.3], "XYStage": [ 37698.4, 32041.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos4", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.16], "Focus": [17741.5], "XYStage": [ 32375.4, 31758.9 ] }, "GridColumnIndex": 0 }, { "Label": "Pos5", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.17000000000002], "Focus": [17741.3], "XYStage": [ 31770.7, 31758.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos6", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.4], "XYStage": [ 26536.800000000003, 31068.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos7", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.205], "Focus": [17741.4], "XYStage": [ 25420.2, 31068.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos8", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.85], "Focus": [17741.3], "XYStage": [ 21059, 31508 ] }, "GridColumnIndex": 0 }, { "Label": "Pos9", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.82], "Focus": [17741.3], "XYStage": [ 19731.9, 31506.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos10", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.79], "Focus": [17741.4], "XYStage": [ 14528.5, 32007.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos11", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.83], "Focus": [17741.2], "XYStage": [ 13830.300000000001, 32009.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos12", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.79], "Focus": [17741.3], "XYStage": [ 8775.300000000001, 32017.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos13", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.815], "Focus": [17741.3], "XYStage": [ 8109.200000000001, 32008.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos14", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.805], "Focus": [17741.3], "XYStage": [ 3483.3, 31908.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos15", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.82], "Focus": [17741.2], "XYStage": [ 2686.8, 31903.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos16", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [211.48000000000002], "Focus": [17741.1], "XYStage": [ -1978.9, 31973.600000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos17", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [211.51500000000001], "Focus": [17741.1], "XYStage": [ -2957.8, 31982.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos18", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.195], "Focus": [17741.5], "XYStage": [ 47747.4, -576.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos19", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.23000000000002], "Focus": [17741.6], "XYStage": [ 47053.9, -576.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos20", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.205], "Focus": [17741.4], "XYStage": [ 41871.200000000004, -479.8 ] }, "GridColumnIndex": 0 }, { "Label": "Pos21", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.4], "XYStage": [ 40882.600000000006, -392 ] }, "GridColumnIndex": 0 }, { "Label": "Pos22", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.24], "Focus": [17741.4], "XYStage": [ 35905.6, -135.3 ] }, "GridColumnIndex": 0 }, { "Label": "Pos23", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.195], "Focus": [17741.5], "XYStage": [ 34990.9, -132.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos24", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.46], "Focus": [17741.4], "XYStage": [ 29678.100000000002, -958.1 ] }, "GridColumnIndex": 0 }, { "Label": "Pos25", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.44], "Focus": [17741.5], "XYStage": [ 28983.600000000002, -939.4000000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos26", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.475], "Focus": [17741.5], "XYStage": [ 23019.7, -122 ] }, "GridColumnIndex": 0 }, { "Label": "Pos27", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.44], "Focus": [17741.7], "XYStage": [ 22428, -116.80000000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos28", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.46], "Focus": [17741.7], "XYStage": [ 16364.900000000001, -181.10000000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos29", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.48000000000002], "Focus": [17741.5], "XYStage": [ 15712.1, -180 ] }, "GridColumnIndex": 0 }, { "Label": "Pos30", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.45000000000002], "Focus": [17741.7], "XYStage": [ 10346.1, 77.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos31", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.49], "Focus": [17741.4], "XYStage": [ 9755.5, 85.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos32", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.475], "Focus": [17741.5], "XYStage": [ 4775.5, 293.90000000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos33", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.445], "Focus": [17741.4], "XYStage": [ 3954.9, 293.90000000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos34", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.455], "Focus": [17741.4], "XYStage": [ -1324.9, 545.6 ] }, "GridColumnIndex": 0 }, { "Label": "Pos35", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.445], "Focus": [17741.3], "XYStage": [ -2097, 576.6 ] }, "GridColumnIndex": 0 } ], "z-step_um": 10, "GridRow": 0, "BitDepth": 16, "Depth": 2, "Channels": 6, "Source": "Micro-Manager", "ChColors": [ -16711681, -256, -65281, -1, -1, -16776961 ], "MetadataVersion": 10, "KeepShutterOpenChannels": true, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536, 65536 ], "CustomIntervals_ms": [], "Height": 1200, "MicroManagerVersion": "1.4.23 20180219", "GridColumn": 0, "UUID": "18808e7c-f2e6-47b3-90d8-1d779ce87dbd", "PixelType": "GRAY16", "Width": 1200, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\Yan\\20191218-YC113-S7S8-miseq", "Frames": 1, "Comment": "", "UserName": "Nikon", "PixelSize_um": 1.1, "ROI": [ 0, 0, 1200, 1200 ], "Date": "2019-12-19", "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC", "P-DAPI-high" ], "KeepShutterOpenSlices": true, "IJType": 1, "ComputerName": "ZadorConfocal2", "Positions": 36, "PositionIndex": 34, "CameraTimeout": 20000, "TimeFirst": false }, "640 AO-Name": "AnalogIO", "Vincent-D3-Command": "Close" }, "FrameKey-0-2-4": { "COM4-StopBits": "1", "528 AO-MaxVolts": "5.0000", "XLIGHT Excitation Wheel-State": "1", "470 Shutter-Name": "AnalogIO", "Core-Shutter": "640", "555 AO-Demo": "No", "XLIGHT Dichroic-State": "2", "XYStage-StepSize-Y": "0.1000", "ZPositionUm": 208.455, "XYStage-StepSize-X": "0.1000", "PRIME-FirmwareVersion": "20.28", "PRIME-ClearCycles": "2", "520-Name": "DA Shutter", "405 Shutter-Name": "AnalogIO", "PIEZO-StepSize": "0.0050", "PRIME-ClearMode": "Pre-Exposure", "470 AO-MinVolts": "0.0000", "405 AO-SupportsTriggering": "1", "CameraChannelIndex": 0, "XYStage-StartSpeed": "500.0000", "COM9-StopBits": "2", "405 AO-Description": "NI DAC", "COM4-DelayBetweenCharsMs": "0.0000", "445 AO-Block voltage": "false", "520 AO-TriggerInputLine": "/Dev1/PFI0", "555 AO-Description": "NI DAC", "520 AO-TriggerSampleRate": "100000", "COM4-Verbose": "1", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM4-Name": "COM4", "405 Shutter-Volts": "0.0000", "Vincent-D3-Port": "COM1", "PRIME-PP 4 MIN ADU AFFECTED": "200", "470 Shutter-MinVolts": "0.0000", "PRIME-PP 3 ENABLED": "Yes", "470 AO-Description": "NI DAC", "PRIME-Description": "PVCAM API device adapter", "520 Shutter-MaxVolts": "5.0000", "520 Shutter-Volts": "0.0000", "528 AO-Sequenceable": "1", "PRIME-Binning": "1x1", "555 Shutter-MaxVolts": "5.0000", "528 Shutter-TriggerSequenceLength": "1000", "405 Shutter-Block voltage": "false", "XLIGHT Spin motor-State": "1", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "Vincent-D3-Time to open (ms)": "35.0000", "Vincent-D3-Time to close (ms)": "35.0000", "445 AO-TriggerSampleRate": "100000", "640 Shutter-IOChannel": "Dev1/ao14", "555 AO-Sequenceable": "1", "520 AO-Name": "AnalogIO", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "470 AO-Block voltage": "false", "COM1-Verbose": "1", "COM9-AnswerTimeout": "10000.0000", "528 Shutter-Name": "AnalogIO", "PRIME-Trigger Timeout (secs)": "10", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "NextFrame": 0, "470 AO-TriggerSequenceLength": "1000", "PRIME-TransposeXY": "0", "445 AO-TriggerSequenceLength": "1000", "COM1-Name": "COM1", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "COM9-DataBits": "8", "COM1-AnswerTimeout": "500.0000", "Camera": "", "TE2000-Version": "V2.50-V2.50-V2.10", "528 Shutter-Volts": "0.0000", "XLIGHT Hub-Name": "XLIGHT Hub", "PIEZO-Description": "Ludl stage driver adapter", "PositionName": "Pos34", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSampleRate": "100000", "PRIME-FanSpeedSetpoint": "High", "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "405 AO-TriggerSampleRate": "100000", "470 Shutter-Block voltage": "false", "470-State": "0", "COM3-Parity": "None", "405 AO-Volts": "0.0000", "PRIME-TransposeMirrorY": "0", "PRIME-ChipName": "GS144BSI", "470 AO-Volts": "0.0000", "PRIME-TransposeMirrorX": "0", "445 Shutter-SupportsTriggering": "0", "470 Shutter-SupportsTriggering": "0", "XLIGHT Hub-Description": "XLIGHT hub", "PRIME-PreampOffLimit": "10000", "470 AO-Sequenceable": "1", "528 Shutter-MaxVolts": "5.0000", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "Core-XYStage": "XYStage", "470 AO-TriggerSampleRate": "100000", "PRIME-Timing-PreTriggerDelayNs": "24672000.0000", "528-DA Device": "528 Shutter", "Focus-Description": "Nikon TE2000 focus adapter", "FilterBlock-State": "5", "520 AO-Demo": "No", "COM3-DelayBetweenCharsMs": "0.0000", "470 Shutter-Volts": "0.0000", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "PRIME-PP 2 THRESHOLD": "125", "PRIME-PP 4 THRESHOLD": "75", "XYStage-Acceleration": "4.0000", "470 Shutter-Demo": "No", "PRIME-Name": "PMPCIECam00", "640-DA Device": "640 Shutter", "405 AO-Block voltage": "false", "ElapsedTime-ms": 1405633, "PRIME-Offset": "100", "445 AO-IOChannel": "Dev1/ao1", "640 AO-MaxVolts": "5.0000", "PositionIndex": 34, "PRIME-PP 1 ENABLED": "Yes", "COM1-BaudRate": "9600", "640-State": "1", "COM3-Verbose": "1", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "LudlController-TransmissionDelay": "0", "PRIME-FullWellCapacity": "62000", "PRIME-SMARTStreamingEnabled": "No", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "640 Shutter-Description": "NI DAC", "528-Description": "DA device that is used as a shutter", "COM4-Description": "Serial port driver (boost:asio)", "405 Shutter-Description": "NI DAC", "TE2000-Name": "TE2000", "528 Shutter-Description": "NI DAC", "COM9-Description": "Serial port driver (boost:asio)", "LudlController-Name": "LudlController", "445 Shutter-TriggerSequenceLength": "1000", "PRIME-SerialNumber": "A18B203008", "445 AO-Volts": "0.0000", "PRIME-X-dimension": "1200", "PRIME-ShutterCloseDelay": "0", "XLIGHT Disk slider-State": "2", "Position": "Default", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 AO-IOChannel": "Dev1/ao5", "520 AO-IOChannel": "Dev1/ao4", "528 AO-TriggerSequenceLength": "1000", "405 Shutter-MaxVolts": "5.0000", "470-DA Device": "470 Shutter", "PRIME-FTCapable": "No", "Core-ChannelGroup": "Channels", "555 AO-SupportsTriggering": "0", "PRIME-Y-dimension": "1200", "Frame": 0, "555 Shutter-Sequenceable": "1", "PRIME-Timing-ExposureTimeNs": "499998000.0000", "Core-ImageProcessor": "", "528-State": "0", "520 AO-Block voltage": "false", "XLIGHT Excitation Wheel-Label": "QUAD", "640 Shutter-Demo": "No", "COM3-StopBits": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "520 Shutter-IOChannel": "Dev1/ao11", "Width": 1200, "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-SupportsTriggering": "0", "640 AO-TriggerSampleRate": "100000", "520-Description": "DA device that is used as a shutter", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "405 Shutter-Sequenceable": "1", "405 Shutter-SupportsTriggering": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-TriggerSampleRate": "100000", "640 Shutter-MaxVolts": "5.0000", "555 AO-MaxVolts": "5.0000", "520 AO-TriggerSequenceLength": "1000", "COM3-BaudRate": "9600", "COM9-BaudRate": "9600", "Vincent-D3-Channel #": "Ch. #1", "PRIME-PMode": "Normal", "OpticalPath-Label": "Left(100)", "BitDepth": 16, "555 AO-TriggerSequenceLength": "1000", "445 Shutter-Description": "NI DAC", "405 AO-Sequenceable": "1", "PRIME-AcquisitionMethod": "Callbacks", "555 AO-Name": "AnalogIO", "PRIME-ShutterMode": "Pre-Exposure", "COM3-Handshaking": "Off", "445 AO-SupportsTriggering": "0", "ROI": "0-0-1200-1200", "COM9-Verbose": "1", "PRIME-Color": "OFF", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "PRIME-ShutterOpenDelay": "0", "COM1-Parity": "None", "Slice": 4, "SlicePosition": -10, "COM9-DelayBetweenCharsMs": "0.0000", "555-Name": "DA Shutter", "PIEZO-LudlSingleAxisName": "B", "XLIGHT Emission Wheel-Label": "679/29 (A)", "640 Shutter-Name": "AnalogIO", "640 AO-Sequenceable": "1", "520 Shutter-Demo": "No", "528 Shutter-Demo": "No", "470-Name": "DA Shutter", "520-DA Device": "520 Shutter", "XYStage-Description": "Ludl XY stage driver adapter", "445 Shutter-MinVolts": "0.0000", "528 AO-TriggerSampleRate": "100000", "405 AO-MinVolts": "0.0000", "405 Shutter-TriggerSequenceLength": "1000", "Height": 1200, "555 AO-Block voltage": "false", "640 Shutter-Volts": "5.0000", "PixelType": "GRAY16", "445 Shutter-Block voltage": "false", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "520 Shutter-TriggerSequenceLength": "1000", "COM9-Name": "COM9", "Core-SLM": "", "555 AO-Volts": "0.0000", "555 Shutter-Block voltage": "false", "XYStage-Speed": "1800.0000", "520 AO-MaxVolts": "5.0000", "528 Shutter-SupportsTriggering": "0", "COM1-Description": "Serial port driver (boost:asio)", "PRIME-MetadataEnabled": "No", "520 Shutter-MinVolts": "0.0000", "470 Shutter-Sequenceable": "1", "PRIME-PreampDelay": "0", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-MinVolts": "0.0000", "ChannelIndex": 2, "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Core-AutoShutter": "1", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "Exposure-ms": 320, "PRIME-PP 2 ENABLED": "Yes", "640 Shutter-TriggerSampleRate": "100000", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "470 Shutter-IOChannel": "Dev1/ao10", "405 AO-Demo": "No", "PixelSizeUm": 1.1, "470 AO-Demo": "No", "470 Shutter-MaxVolts": "5.0000", "Vincent-D3-Name": "Vincent-D3", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FileName": "img_000000000_Il-A_004.tif", "LudlController-Reset": "Operate", "PRIME-Exposure": "320.00", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "445-Name": "DA Shutter", "555 Shutter-Name": "AnalogIO", "520 Shutter-TriggerSampleRate": "100000", "445 Shutter-IOChannel": "Dev1/ao9", "PRIME-TransposeCorrection": "0", "XYStage-StepSize": "0.1000", "445-Description": "DA device that is used as a shutter", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 AO-IOChannel": "Dev1/ao7", "520 Shutter-Name": "AnalogIO", "COM3-DataBits": "8", "640 AO-Volts": "4.5000", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "555 Shutter-IOChannel": "Dev1/ao13", "WaitInterval": null, "520 Shutter-Sequenceable": "1", "YPositionUm": 545.6, "445-State": "0", "445-DA Device": "445 Shutter", "PRIME-CircularBufferAutoSize": "ON", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "555 AO-TriggerSampleRate": "100000", "445 Shutter-Volts": "0.0000", "XLIGHT Hub-Port": "COM3", "Core-AutoFocus": "", "COM4-Handshaking": "Off", "528 Shutter-MinVolts": "0.0000", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "445 Shutter-Name": "AnalogIO", "528 Shutter-Sequenceable": "1", "XYStage-TransposeMirrorX": "0", "XYStage-TransposeMirrorY": "0", "PRIME-ReadoutRate": "100MHz 16bit", "XYStage-Name": "Stage", "640 AO-SupportsTriggering": "0", "640-Name": "DA Shutter", "640 Shutter-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "640 AO-Block voltage": "false", "445 Shutter-Sequenceable": "1", "470 AO-MaxVolts": "5.0000", "COM4-BaudRate": "9600", "470-Description": "DA device that is used as a shutter", "520 Shutter-Description": "NI DAC", "PRIME-CCDTemperature": "-14.8500", "Time": "2019-12-19 09:59:36 -0500", "640 AO-TriggerSequenceLength": "1000", "470 AO-Name": "AnalogIO", "XLIGHT Disk slider-Label": "Disk Pos 40um", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "Binning": "1x1", "TE2000-ModelType": "1", "COM3-AnswerTimeout": "500.0000", "555 Shutter-SupportsTriggering": "0", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "TE2000-Port": "COM4", "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Description": "NI DAC", "COM4-AnswerTimeout": "500.0000", "COM1-DelayBetweenCharsMs": "0.0000", "FilterBlock-Label": "Confocal", "445 AO-Name": "AnalogIO", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-Timing-ReadoutTimeNs": "24672000.0000", "528 AO-Demo": "No", "520 AO-Sequenceable": "1", "555-Description": "DA device that is used as a shutter", "405-DA Device": "405 Shutter", "405 AO-TriggerSequenceLength": "1000", "640 AO-MinVolts": "0.0000", "640 Shutter-Block voltage": "false", "FrameIndex": 0, "PRIME-Port": "CMOS", "555 AO-MinVolts": "0.0000", "COM3-Description": "Serial port driver (boost:asio)", "528 AO-Description": "NI DAC", "555 AO-IOChannel": "Dev1/ao6", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "555 AO-TriggerInputLine": "/Dev1/PFI0", "PRIME-ActualInterval-ms": "390.5200", "LudlController-Port": "COM9", "445 Shutter-Demo": "No", "PRIME-Trigger-Expose Out-Mux": "1", "SliceIndex": 4, "PRIME-CCDTemperatureSetPoint": "-15.0000", "405 Shutter-MinVolts": "0.0000", "PRIME-ExposeOutMode": "First Row", "AxisPositions": null, "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Volts": "0.0000", "528-Name": "DA Shutter", "555-State": "0", "XLIGHT Dichroic-Label": "Di03-R635-t1", "445 AO-Sequenceable": "1", "405-Name": "DA Shutter", "405 AO-IOChannel": "Dev1/ao0", "Core-Camera": "PRIME", "Focus-Name": "Focus", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "405 Shutter-IOChannel": "Dev1/ao8", "640 AO-Demo": "No", "640 Shutter-SupportsTriggering": "0", "445 AO-Demo": "No", "555-DA Device": "555 Shutter", "470 Shutter-Description": "NI DAC", "528 AO-Volts": "0.0000", "COM1-DataBits": "8", "Vincent-D3-Address": "x", "520 AO-SupportsTriggering": "0", "405-Description": "DA device that is used as a shutter", "640 Shutter-MinVolts": "0.0000", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "405 AO-Name": "AnalogIO", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "640 AO-TriggerInputLine": "/Dev1/PFI0", "405 AO-TriggerInputLine": "/Dev1/PFI0", "640-Description": "DA device that is used as a shutter", "445 Shutter-TriggerSampleRate": "100000", "470 Shutter-TriggerSequenceLength": "1000", "COM1-StopBits": "1", "520 Shutter-SupportsTriggering": "0", "405-State": "0", "Core-Initialize": "1", "XLIGHT Emission Wheel-State": "3", "520 AO-Description": "NI DAC", "528 AO-TriggerInputLine": "/Dev1/PFI0", "445 Shutter-MaxVolts": "5.0000", "COM9-Handshaking": "Off", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "XLIGHT Spin motor-Label": "On (spinning)", "PRIME-PP 5 ENABLED": "No", "UUID": "d23d28d1-3ad6-4e44-8c54-06eb244cfd71", "PRIME-TriggerMode": "Internal Trigger", "COM1-Handshaking": "Off", "PRIME-Timing-PostTriggerDelayNs": "49344000.0000", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "Focus-Position": "17741.5000", "528 Shutter-Block voltage": "false", "Channel": "Il-A", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "555 Shutter-Demo": "No", "405 Shutter-Demo": "No", "528 AO-Name": "AnalogIO", "520-State": "0", "Core-Galvo": "", "405 Shutter-TriggerSampleRate": "100000", "Focus-FocusStepSize_(nm)": "100", "PRIME-PixelType": "16bit", "COM4-DataBits": "8", "470 AO-IOChannel": "Dev1/ao2", "520 AO-MinVolts": "0.0000", "COM9-Parity": "None", "PRIME-CircularBufferEnabled": "ON", "PRIME-PP 6 Reset": "No", "640 AO-Description": "NI DAC", "Source": "PRIME", "445 AO-MinVolts": "0.0000", "PIEZO-Name": "Stage", "445 AO-Description": "NI DAC", "PRIME-CircularBufferFrameCount": "46", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "520 AO-Volts": "0.0000", "528 Shutter-TriggerSampleRate": "100000", "XPositionUm": -1324.9, "OpticalPath-State": "4", "PRIME-PP 3 THRESHOLD": "80", "PRIME-PVCAM Version": "3.7.1", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Gain": "1-HDR", "640 Shutter-TriggerSequenceLength": "1000", "528 AO-SupportsTriggering": "0", "445 AO-TriggerInputLine": "/Dev1/PFI0", "OpticalPath-Name": "OpticalPath", "PRIME-PP 4 ENABLED": "Yes", "PRIME-PVCAM Adapter Version": "1.3.29", "COM3-Name": "COM3", "405 AO-MaxVolts": "5.0000", "520 Shutter-Block voltage": "false", "PRIME-Timing-ClearingTimeNs": "49344000.0000", "Core-Focus": "PIEZO", "528 AO-Block voltage": "false", "TE2000-Description": "Nikon TE2000 hub", "528 AO-MinVolts": "0.0000", "COM4-Parity": "None", "Summary": { "Slices": 11, "ChContrastMin": [ 0, 0, 0, 0, 0, 0 ], "PixelAspect": 1, "SlicesFirst": true, "Interval_ms": 0, "Time": "2019-12-19 09:59:21 -0500", "Prefix": "seq_13", "InitialPositionList": [ { "Label": "Pos0", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.21], "Focus": [17741.5], "XYStage": [ 44542.200000000004, 31636.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos1", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.22], "Focus": [17741.4], "XYStage": [ 43801.100000000006, 31637.9 ] }, "GridColumnIndex": 0 }, { "Label": "Pos2", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.5], "XYStage": [ 38561.3, 32040.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos3", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.185], "Focus": [17741.3], "XYStage": [ 37698.4, 32041.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos4", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.16], "Focus": [17741.5], "XYStage": [ 32375.4, 31758.9 ] }, "GridColumnIndex": 0 }, { "Label": "Pos5", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.17000000000002], "Focus": [17741.3], "XYStage": [ 31770.7, 31758.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos6", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.4], "XYStage": [ 26536.800000000003, 31068.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos7", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.205], "Focus": [17741.4], "XYStage": [ 25420.2, 31068.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos8", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.85], "Focus": [17741.3], "XYStage": [ 21059, 31508 ] }, "GridColumnIndex": 0 }, { "Label": "Pos9", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.82], "Focus": [17741.3], "XYStage": [ 19731.9, 31506.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos10", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.79], "Focus": [17741.4], "XYStage": [ 14528.5, 32007.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos11", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.83], "Focus": [17741.2], "XYStage": [ 13830.300000000001, 32009.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos12", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.79], "Focus": [17741.3], "XYStage": [ 8775.300000000001, 32017.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos13", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.815], "Focus": [17741.3], "XYStage": [ 8109.200000000001, 32008.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos14", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.805], "Focus": [17741.3], "XYStage": [ 3483.3, 31908.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos15", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.82], "Focus": [17741.2], "XYStage": [ 2686.8, 31903.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos16", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [211.48000000000002], "Focus": [17741.1], "XYStage": [ -1978.9, 31973.600000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos17", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [211.51500000000001], "Focus": [17741.1], "XYStage": [ -2957.8, 31982.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos18", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.195], "Focus": [17741.5], "XYStage": [ 47747.4, -576.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos19", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.23000000000002], "Focus": [17741.6], "XYStage": [ 47053.9, -576.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos20", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.205], "Focus": [17741.4], "XYStage": [ 41871.200000000004, -479.8 ] }, "GridColumnIndex": 0 }, { "Label": "Pos21", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.4], "XYStage": [ 40882.600000000006, -392 ] }, "GridColumnIndex": 0 }, { "Label": "Pos22", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.24], "Focus": [17741.4], "XYStage": [ 35905.6, -135.3 ] }, "GridColumnIndex": 0 }, { "Label": "Pos23", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.195], "Focus": [17741.5], "XYStage": [ 34990.9, -132.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos24", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.46], "Focus": [17741.4], "XYStage": [ 29678.100000000002, -958.1 ] }, "GridColumnIndex": 0 }, { "Label": "Pos25", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.44], "Focus": [17741.5], "XYStage": [ 28983.600000000002, -939.4000000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos26", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.475], "Focus": [17741.5], "XYStage": [ 23019.7, -122 ] }, "GridColumnIndex": 0 }, { "Label": "Pos27", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.44], "Focus": [17741.7], "XYStage": [ 22428, -116.80000000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos28", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.46], "Focus": [17741.7], "XYStage": [ 16364.900000000001, -181.10000000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos29", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.48000000000002], "Focus": [17741.5], "XYStage": [ 15712.1, -180 ] }, "GridColumnIndex": 0 }, { "Label": "Pos30", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.45000000000002], "Focus": [17741.7], "XYStage": [ 10346.1, 77.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos31", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.49], "Focus": [17741.4], "XYStage": [ 9755.5, 85.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos32", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.475], "Focus": [17741.5], "XYStage": [ 4775.5, 293.90000000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos33", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.445], "Focus": [17741.4], "XYStage": [ 3954.9, 293.90000000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos34", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.455], "Focus": [17741.4], "XYStage": [ -1324.9, 545.6 ] }, "GridColumnIndex": 0 }, { "Label": "Pos35", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.445], "Focus": [17741.3], "XYStage": [ -2097, 576.6 ] }, "GridColumnIndex": 0 } ], "z-step_um": 10, "GridRow": 0, "BitDepth": 16, "Depth": 2, "Channels": 6, "Source": "Micro-Manager", "ChColors": [ -16711681, -256, -65281, -1, -1, -16776961 ], "MetadataVersion": 10, "KeepShutterOpenChannels": true, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536, 65536 ], "CustomIntervals_ms": [], "Height": 1200, "MicroManagerVersion": "1.4.23 20180219", "GridColumn": 0, "UUID": "18808e7c-f2e6-47b3-90d8-1d779ce87dbd", "PixelType": "GRAY16", "Width": 1200, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\Yan\\20191218-YC113-S7S8-miseq", "Frames": 1, "Comment": "", "UserName": "Nikon", "PixelSize_um": 1.1, "ROI": [ 0, 0, 1200, 1200 ], "Date": "2019-12-19", "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC", "P-DAPI-high" ], "KeepShutterOpenSlices": true, "IJType": 1, "ComputerName": "ZadorConfocal2", "Positions": 36, "PositionIndex": 34, "CameraTimeout": 20000, "TimeFirst": false }, "640 AO-Name": "AnalogIO", "Vincent-D3-Command": "Close" }, "FrameKey-0-2-5": { "COM4-StopBits": "1", "528 AO-MaxVolts": "5.0000", "XLIGHT Excitation Wheel-State": "1", "470 Shutter-Name": "AnalogIO", "Core-Shutter": "640", "555 AO-Demo": "No", "XLIGHT Dichroic-State": "2", "XYStage-StepSize-Y": "0.1000", "ZPositionUm": 218.455, "XYStage-StepSize-X": "0.1000", "PRIME-FirmwareVersion": "20.28", "PRIME-ClearCycles": "2", "520-Name": "DA Shutter", "405 Shutter-Name": "AnalogIO", "PIEZO-StepSize": "0.0050", "PRIME-ClearMode": "Pre-Exposure", "470 AO-MinVolts": "0.0000", "405 AO-SupportsTriggering": "1", "CameraChannelIndex": 0, "XYStage-StartSpeed": "500.0000", "COM9-StopBits": "2", "405 AO-Description": "NI DAC", "COM4-DelayBetweenCharsMs": "0.0000", "445 AO-Block voltage": "false", "520 AO-TriggerInputLine": "/Dev1/PFI0", "555 AO-Description": "NI DAC", "520 AO-TriggerSampleRate": "100000", "COM4-Verbose": "1", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM4-Name": "COM4", "405 Shutter-Volts": "0.0000", "Vincent-D3-Port": "COM1", "PRIME-PP 4 MIN ADU AFFECTED": "200", "470 Shutter-MinVolts": "0.0000", "PRIME-PP 3 ENABLED": "Yes", "470 AO-Description": "NI DAC", "PRIME-Description": "PVCAM API device adapter", "520 Shutter-MaxVolts": "5.0000", "520 Shutter-Volts": "0.0000", "528 AO-Sequenceable": "1", "PRIME-Binning": "1x1", "555 Shutter-MaxVolts": "5.0000", "528 Shutter-TriggerSequenceLength": "1000", "405 Shutter-Block voltage": "false", "XLIGHT Spin motor-State": "1", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "Vincent-D3-Time to open (ms)": "35.0000", "Vincent-D3-Time to close (ms)": "35.0000", "445 AO-TriggerSampleRate": "100000", "640 Shutter-IOChannel": "Dev1/ao14", "555 AO-Sequenceable": "1", "520 AO-Name": "AnalogIO", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "470 AO-Block voltage": "false", "COM1-Verbose": "1", "COM9-AnswerTimeout": "10000.0000", "528 Shutter-Name": "AnalogIO", "PRIME-Trigger Timeout (secs)": "10", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "NextFrame": 0, "470 AO-TriggerSequenceLength": "1000", "PRIME-TransposeXY": "0", "445 AO-TriggerSequenceLength": "1000", "COM1-Name": "COM1", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "COM9-DataBits": "8", "COM1-AnswerTimeout": "500.0000", "Camera": "", "TE2000-Version": "V2.50-V2.50-V2.10", "528 Shutter-Volts": "0.0000", "XLIGHT Hub-Name": "XLIGHT Hub", "PIEZO-Description": "Ludl stage driver adapter", "PositionName": "Pos34", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSampleRate": "100000", "PRIME-FanSpeedSetpoint": "High", "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "405 AO-TriggerSampleRate": "100000", "470 Shutter-Block voltage": "false", "470-State": "0", "COM3-Parity": "None", "405 AO-Volts": "0.0000", "PRIME-TransposeMirrorY": "0", "PRIME-ChipName": "GS144BSI", "470 AO-Volts": "0.0000", "PRIME-TransposeMirrorX": "0", "445 Shutter-SupportsTriggering": "0", "470 Shutter-SupportsTriggering": "0", "XLIGHT Hub-Description": "XLIGHT hub", "PRIME-PreampOffLimit": "10000", "470 AO-Sequenceable": "1", "528 Shutter-MaxVolts": "5.0000", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "Core-XYStage": "XYStage", "470 AO-TriggerSampleRate": "100000", "PRIME-Timing-PreTriggerDelayNs": "24672000.0000", "528-DA Device": "528 Shutter", "Focus-Description": "Nikon TE2000 focus adapter", "FilterBlock-State": "5", "520 AO-Demo": "No", "COM3-DelayBetweenCharsMs": "0.0000", "470 Shutter-Volts": "0.0000", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "PRIME-PP 2 THRESHOLD": "125", "PRIME-PP 4 THRESHOLD": "75", "XYStage-Acceleration": "4.0000", "470 Shutter-Demo": "No", "PRIME-Name": "PMPCIECam00", "640-DA Device": "640 Shutter", "405 AO-Block voltage": "false", "ElapsedTime-ms": 1406193, "PRIME-Offset": "100", "445 AO-IOChannel": "Dev1/ao1", "640 AO-MaxVolts": "5.0000", "PositionIndex": 34, "PRIME-PP 1 ENABLED": "Yes", "COM1-BaudRate": "9600", "640-State": "1", "COM3-Verbose": "1", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "LudlController-TransmissionDelay": "0", "PRIME-FullWellCapacity": "62000", "PRIME-SMARTStreamingEnabled": "No", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "640 Shutter-Description": "NI DAC", "528-Description": "DA device that is used as a shutter", "COM4-Description": "Serial port driver (boost:asio)", "405 Shutter-Description": "NI DAC", "TE2000-Name": "TE2000", "528 Shutter-Description": "NI DAC", "COM9-Description": "Serial port driver (boost:asio)", "LudlController-Name": "LudlController", "445 Shutter-TriggerSequenceLength": "1000", "PRIME-SerialNumber": "A18B203008", "445 AO-Volts": "0.0000", "PRIME-X-dimension": "1200", "PRIME-ShutterCloseDelay": "0", "XLIGHT Disk slider-State": "2", "Position": "Default", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 AO-IOChannel": "Dev1/ao5", "520 AO-IOChannel": "Dev1/ao4", "528 AO-TriggerSequenceLength": "1000", "405 Shutter-MaxVolts": "5.0000", "470-DA Device": "470 Shutter", "PRIME-FTCapable": "No", "Core-ChannelGroup": "Channels", "555 AO-SupportsTriggering": "0", "PRIME-Y-dimension": "1200", "Frame": 0, "555 Shutter-Sequenceable": "1", "PRIME-Timing-ExposureTimeNs": "499998000.0000", "Core-ImageProcessor": "", "528-State": "0", "520 AO-Block voltage": "false", "XLIGHT Excitation Wheel-Label": "QUAD", "640 Shutter-Demo": "No", "COM3-StopBits": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "520 Shutter-IOChannel": "Dev1/ao11", "Width": 1200, "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-SupportsTriggering": "0", "640 AO-TriggerSampleRate": "100000", "520-Description": "DA device that is used as a shutter", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "405 Shutter-Sequenceable": "1", "405 Shutter-SupportsTriggering": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-TriggerSampleRate": "100000", "640 Shutter-MaxVolts": "5.0000", "555 AO-MaxVolts": "5.0000", "520 AO-TriggerSequenceLength": "1000", "COM3-BaudRate": "9600", "COM9-BaudRate": "9600", "Vincent-D3-Channel #": "Ch. #1", "PRIME-PMode": "Normal", "OpticalPath-Label": "Left(100)", "BitDepth": 16, "555 AO-TriggerSequenceLength": "1000", "445 Shutter-Description": "NI DAC", "405 AO-Sequenceable": "1", "PRIME-AcquisitionMethod": "Callbacks", "555 AO-Name": "AnalogIO", "PRIME-ShutterMode": "Pre-Exposure", "COM3-Handshaking": "Off", "445 AO-SupportsTriggering": "0", "ROI": "0-0-1200-1200", "COM9-Verbose": "1", "PRIME-Color": "OFF", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "PRIME-ShutterOpenDelay": "0", "COM1-Parity": "None", "Slice": 5, "SlicePosition": 0, "COM9-DelayBetweenCharsMs": "0.0000", "555-Name": "DA Shutter", "PIEZO-LudlSingleAxisName": "B", "XLIGHT Emission Wheel-Label": "679/29 (A)", "640 Shutter-Name": "AnalogIO", "640 AO-Sequenceable": "1", "520 Shutter-Demo": "No", "528 Shutter-Demo": "No", "470-Name": "DA Shutter", "520-DA Device": "520 Shutter", "XYStage-Description": "Ludl XY stage driver adapter", "445 Shutter-MinVolts": "0.0000", "528 AO-TriggerSampleRate": "100000", "405 AO-MinVolts": "0.0000", "405 Shutter-TriggerSequenceLength": "1000", "Height": 1200, "555 AO-Block voltage": "false", "640 Shutter-Volts": "5.0000", "PixelType": "GRAY16", "445 Shutter-Block voltage": "false", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "520 Shutter-TriggerSequenceLength": "1000", "COM9-Name": "COM9", "Core-SLM": "", "555 AO-Volts": "0.0000", "555 Shutter-Block voltage": "false", "XYStage-Speed": "1800.0000", "520 AO-MaxVolts": "5.0000", "528 Shutter-SupportsTriggering": "0", "COM1-Description": "Serial port driver (boost:asio)", "PRIME-MetadataEnabled": "No", "520 Shutter-MinVolts": "0.0000", "470 Shutter-Sequenceable": "1", "PRIME-PreampDelay": "0", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-MinVolts": "0.0000", "ChannelIndex": 2, "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Core-AutoShutter": "1", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "Exposure-ms": 320, "PRIME-PP 2 ENABLED": "Yes", "640 Shutter-TriggerSampleRate": "100000", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "470 Shutter-IOChannel": "Dev1/ao10", "405 AO-Demo": "No", "PixelSizeUm": 1.1, "470 AO-Demo": "No", "470 Shutter-MaxVolts": "5.0000", "Vincent-D3-Name": "Vincent-D3", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FileName": "img_000000000_Il-A_005.tif", "LudlController-Reset": "Operate", "PRIME-Exposure": "320.00", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "445-Name": "DA Shutter", "555 Shutter-Name": "AnalogIO", "520 Shutter-TriggerSampleRate": "100000", "445 Shutter-IOChannel": "Dev1/ao9", "PRIME-TransposeCorrection": "0", "XYStage-StepSize": "0.1000", "445-Description": "DA device that is used as a shutter", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 AO-IOChannel": "Dev1/ao7", "520 Shutter-Name": "AnalogIO", "COM3-DataBits": "8", "640 AO-Volts": "4.5000", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "555 Shutter-IOChannel": "Dev1/ao13", "WaitInterval": null, "520 Shutter-Sequenceable": "1", "YPositionUm": 545.6, "445-State": "0", "445-DA Device": "445 Shutter", "PRIME-CircularBufferAutoSize": "ON", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "555 AO-TriggerSampleRate": "100000", "445 Shutter-Volts": "0.0000", "XLIGHT Hub-Port": "COM3", "Core-AutoFocus": "", "COM4-Handshaking": "Off", "528 Shutter-MinVolts": "0.0000", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "445 Shutter-Name": "AnalogIO", "528 Shutter-Sequenceable": "1", "XYStage-TransposeMirrorX": "0", "XYStage-TransposeMirrorY": "0", "PRIME-ReadoutRate": "100MHz 16bit", "XYStage-Name": "Stage", "640 AO-SupportsTriggering": "0", "640-Name": "DA Shutter", "640 Shutter-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "640 AO-Block voltage": "false", "445 Shutter-Sequenceable": "1", "470 AO-MaxVolts": "5.0000", "COM4-BaudRate": "9600", "470-Description": "DA device that is used as a shutter", "520 Shutter-Description": "NI DAC", "PRIME-CCDTemperature": "-14.8500", "Time": "2019-12-19 09:59:36 -0500", "640 AO-TriggerSequenceLength": "1000", "470 AO-Name": "AnalogIO", "XLIGHT Disk slider-Label": "Disk Pos 40um", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "Binning": "1x1", "TE2000-ModelType": "1", "COM3-AnswerTimeout": "500.0000", "555 Shutter-SupportsTriggering": "0", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "TE2000-Port": "COM4", "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Description": "NI DAC", "COM4-AnswerTimeout": "500.0000", "COM1-DelayBetweenCharsMs": "0.0000", "FilterBlock-Label": "Confocal", "445 AO-Name": "AnalogIO", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-Timing-ReadoutTimeNs": "24672000.0000", "528 AO-Demo": "No", "520 AO-Sequenceable": "1", "555-Description": "DA device that is used as a shutter", "405-DA Device": "405 Shutter", "405 AO-TriggerSequenceLength": "1000", "640 AO-MinVolts": "0.0000", "640 Shutter-Block voltage": "false", "FrameIndex": 0, "PRIME-Port": "CMOS", "555 AO-MinVolts": "0.0000", "COM3-Description": "Serial port driver (boost:asio)", "528 AO-Description": "NI DAC", "555 AO-IOChannel": "Dev1/ao6", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "555 AO-TriggerInputLine": "/Dev1/PFI0", "PRIME-ActualInterval-ms": "390.5200", "LudlController-Port": "COM9", "445 Shutter-Demo": "No", "PRIME-Trigger-Expose Out-Mux": "1", "SliceIndex": 5, "PRIME-CCDTemperatureSetPoint": "-15.0000", "405 Shutter-MinVolts": "0.0000", "PRIME-ExposeOutMode": "First Row", "AxisPositions": null, "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Volts": "0.0000", "528-Name": "DA Shutter", "555-State": "0", "XLIGHT Dichroic-Label": "Di03-R635-t1", "445 AO-Sequenceable": "1", "405-Name": "DA Shutter", "405 AO-IOChannel": "Dev1/ao0", "Core-Camera": "PRIME", "Focus-Name": "Focus", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "405 Shutter-IOChannel": "Dev1/ao8", "640 AO-Demo": "No", "640 Shutter-SupportsTriggering": "0", "445 AO-Demo": "No", "555-DA Device": "555 Shutter", "470 Shutter-Description": "NI DAC", "528 AO-Volts": "0.0000", "COM1-DataBits": "8", "Vincent-D3-Address": "x", "520 AO-SupportsTriggering": "0", "405-Description": "DA device that is used as a shutter", "640 Shutter-MinVolts": "0.0000", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "405 AO-Name": "AnalogIO", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "640 AO-TriggerInputLine": "/Dev1/PFI0", "405 AO-TriggerInputLine": "/Dev1/PFI0", "640-Description": "DA device that is used as a shutter", "445 Shutter-TriggerSampleRate": "100000", "470 Shutter-TriggerSequenceLength": "1000", "COM1-StopBits": "1", "520 Shutter-SupportsTriggering": "0", "405-State": "0", "Core-Initialize": "1", "XLIGHT Emission Wheel-State": "3", "520 AO-Description": "NI DAC", "528 AO-TriggerInputLine": "/Dev1/PFI0", "445 Shutter-MaxVolts": "5.0000", "COM9-Handshaking": "Off", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "XLIGHT Spin motor-Label": "On (spinning)", "PRIME-PP 5 ENABLED": "No", "UUID": "77edea29-088b-4114-b948-6a25242375ab", "PRIME-TriggerMode": "Internal Trigger", "COM1-Handshaking": "Off", "PRIME-Timing-PostTriggerDelayNs": "49344000.0000", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "Focus-Position": "17741.5000", "528 Shutter-Block voltage": "false", "Channel": "Il-A", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "555 Shutter-Demo": "No", "405 Shutter-Demo": "No", "528 AO-Name": "AnalogIO", "520-State": "0", "Core-Galvo": "", "405 Shutter-TriggerSampleRate": "100000", "Focus-FocusStepSize_(nm)": "100", "PRIME-PixelType": "16bit", "COM4-DataBits": "8", "470 AO-IOChannel": "Dev1/ao2", "520 AO-MinVolts": "0.0000", "COM9-Parity": "None", "PRIME-CircularBufferEnabled": "ON", "PRIME-PP 6 Reset": "No", "640 AO-Description": "NI DAC", "Source": "PRIME", "445 AO-MinVolts": "0.0000", "PIEZO-Name": "Stage", "445 AO-Description": "NI DAC", "PRIME-CircularBufferFrameCount": "46", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "520 AO-Volts": "0.0000", "528 Shutter-TriggerSampleRate": "100000", "XPositionUm": -1324.9, "OpticalPath-State": "4", "PRIME-PP 3 THRESHOLD": "80", "PRIME-PVCAM Version": "3.7.1", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Gain": "1-HDR", "640 Shutter-TriggerSequenceLength": "1000", "528 AO-SupportsTriggering": "0", "445 AO-TriggerInputLine": "/Dev1/PFI0", "OpticalPath-Name": "OpticalPath", "PRIME-PP 4 ENABLED": "Yes", "PRIME-PVCAM Adapter Version": "1.3.29", "COM3-Name": "COM3", "405 AO-MaxVolts": "5.0000", "520 Shutter-Block voltage": "false", "PRIME-Timing-ClearingTimeNs": "49344000.0000", "Core-Focus": "PIEZO", "528 AO-Block voltage": "false", "TE2000-Description": "Nikon TE2000 hub", "528 AO-MinVolts": "0.0000", "COM4-Parity": "None", "Summary": { "Slices": 11, "ChContrastMin": [ 0, 0, 0, 0, 0, 0 ], "PixelAspect": 1, "SlicesFirst": true, "Interval_ms": 0, "Time": "2019-12-19 09:59:21 -0500", "Prefix": "seq_13", "InitialPositionList": [ { "Label": "Pos0", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.21], "Focus": [17741.5], "XYStage": [ 44542.200000000004, 31636.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos1", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.22], "Focus": [17741.4], "XYStage": [ 43801.100000000006, 31637.9 ] }, "GridColumnIndex": 0 }, { "Label": "Pos2", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.5], "XYStage": [ 38561.3, 32040.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos3", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.185], "Focus": [17741.3], "XYStage": [ 37698.4, 32041.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos4", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.16], "Focus": [17741.5], "XYStage": [ 32375.4, 31758.9 ] }, "GridColumnIndex": 0 }, { "Label": "Pos5", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.17000000000002], "Focus": [17741.3], "XYStage": [ 31770.7, 31758.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos6", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.4], "XYStage": [ 26536.800000000003, 31068.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos7", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.205], "Focus": [17741.4], "XYStage": [ 25420.2, 31068.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos8", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.85], "Focus": [17741.3], "XYStage": [ 21059, 31508 ] }, "GridColumnIndex": 0 }, { "Label": "Pos9", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.82], "Focus": [17741.3], "XYStage": [ 19731.9, 31506.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos10", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.79], "Focus": [17741.4], "XYStage": [ 14528.5, 32007.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos11", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.83], "Focus": [17741.2], "XYStage": [ 13830.300000000001, 32009.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos12", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.79], "Focus": [17741.3], "XYStage": [ 8775.300000000001, 32017.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos13", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.815], "Focus": [17741.3], "XYStage": [ 8109.200000000001, 32008.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos14", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.805], "Focus": [17741.3], "XYStage": [ 3483.3, 31908.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos15", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.82], "Focus": [17741.2], "XYStage": [ 2686.8, 31903.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos16", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [211.48000000000002], "Focus": [17741.1], "XYStage": [ -1978.9, 31973.600000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos17", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [211.51500000000001], "Focus": [17741.1], "XYStage": [ -2957.8, 31982.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos18", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.195], "Focus": [17741.5], "XYStage": [ 47747.4, -576.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos19", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.23000000000002], "Focus": [17741.6], "XYStage": [ 47053.9, -576.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos20", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.205], "Focus": [17741.4], "XYStage": [ 41871.200000000004, -479.8 ] }, "GridColumnIndex": 0 }, { "Label": "Pos21", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.4], "XYStage": [ 40882.600000000006, -392 ] }, "GridColumnIndex": 0 }, { "Label": "Pos22", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.24], "Focus": [17741.4], "XYStage": [ 35905.6, -135.3 ] }, "GridColumnIndex": 0 }, { "Label": "Pos23", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.195], "Focus": [17741.5], "XYStage": [ 34990.9, -132.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos24", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.46], "Focus": [17741.4], "XYStage": [ 29678.100000000002, -958.1 ] }, "GridColumnIndex": 0 }, { "Label": "Pos25", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.44], "Focus": [17741.5], "XYStage": [ 28983.600000000002, -939.4000000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos26", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.475], "Focus": [17741.5], "XYStage": [ 23019.7, -122 ] }, "GridColumnIndex": 0 }, { "Label": "Pos27", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.44], "Focus": [17741.7], "XYStage": [ 22428, -116.80000000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos28", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.46], "Focus": [17741.7], "XYStage": [ 16364.900000000001, -181.10000000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos29", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.48000000000002], "Focus": [17741.5], "XYStage": [ 15712.1, -180 ] }, "GridColumnIndex": 0 }, { "Label": "Pos30", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.45000000000002], "Focus": [17741.7], "XYStage": [ 10346.1, 77.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos31", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.49], "Focus": [17741.4], "XYStage": [ 9755.5, 85.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos32", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.475], "Focus": [17741.5], "XYStage": [ 4775.5, 293.90000000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos33", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.445], "Focus": [17741.4], "XYStage": [ 3954.9, 293.90000000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos34", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.455], "Focus": [17741.4], "XYStage": [ -1324.9, 545.6 ] }, "GridColumnIndex": 0 }, { "Label": "Pos35", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.445], "Focus": [17741.3], "XYStage": [ -2097, 576.6 ] }, "GridColumnIndex": 0 } ], "z-step_um": 10, "GridRow": 0, "BitDepth": 16, "Depth": 2, "Channels": 6, "Source": "Micro-Manager", "ChColors": [ -16711681, -256, -65281, -1, -1, -16776961 ], "MetadataVersion": 10, "KeepShutterOpenChannels": true, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536, 65536 ], "CustomIntervals_ms": [], "Height": 1200, "MicroManagerVersion": "1.4.23 20180219", "GridColumn": 0, "UUID": "18808e7c-f2e6-47b3-90d8-1d779ce87dbd", "PixelType": "GRAY16", "Width": 1200, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\Yan\\20191218-YC113-S7S8-miseq", "Frames": 1, "Comment": "", "UserName": "Nikon", "PixelSize_um": 1.1, "ROI": [ 0, 0, 1200, 1200 ], "Date": "2019-12-19", "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC", "P-DAPI-high" ], "KeepShutterOpenSlices": true, "IJType": 1, "ComputerName": "ZadorConfocal2", "Positions": 36, "PositionIndex": 34, "CameraTimeout": 20000, "TimeFirst": false }, "640 AO-Name": "AnalogIO", "Vincent-D3-Command": "Close" }, "FrameKey-0-2-6": { "COM4-StopBits": "1", "528 AO-MaxVolts": "5.0000", "XLIGHT Excitation Wheel-State": "1", "470 Shutter-Name": "AnalogIO", "Core-Shutter": "640", "555 AO-Demo": "No", "XLIGHT Dichroic-State": "2", "XYStage-StepSize-Y": "0.1000", "ZPositionUm": 228.455, "XYStage-StepSize-X": "0.1000", "PRIME-FirmwareVersion": "20.28", "PRIME-ClearCycles": "2", "520-Name": "DA Shutter", "405 Shutter-Name": "AnalogIO", "PIEZO-StepSize": "0.0050", "PRIME-ClearMode": "Pre-Exposure", "470 AO-MinVolts": "0.0000", "405 AO-SupportsTriggering": "1", "CameraChannelIndex": 0, "XYStage-StartSpeed": "500.0000", "COM9-StopBits": "2", "405 AO-Description": "NI DAC", "COM4-DelayBetweenCharsMs": "0.0000", "445 AO-Block voltage": "false", "520 AO-TriggerInputLine": "/Dev1/PFI0", "555 AO-Description": "NI DAC", "520 AO-TriggerSampleRate": "100000", "COM4-Verbose": "1", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM4-Name": "COM4", "405 Shutter-Volts": "0.0000", "Vincent-D3-Port": "COM1", "PRIME-PP 4 MIN ADU AFFECTED": "200", "470 Shutter-MinVolts": "0.0000", "PRIME-PP 3 ENABLED": "Yes", "470 AO-Description": "NI DAC", "PRIME-Description": "PVCAM API device adapter", "520 Shutter-MaxVolts": "5.0000", "520 Shutter-Volts": "0.0000", "528 AO-Sequenceable": "1", "PRIME-Binning": "1x1", "555 Shutter-MaxVolts": "5.0000", "528 Shutter-TriggerSequenceLength": "1000", "405 Shutter-Block voltage": "false", "XLIGHT Spin motor-State": "1", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "Vincent-D3-Time to open (ms)": "35.0000", "Vincent-D3-Time to close (ms)": "35.0000", "445 AO-TriggerSampleRate": "100000", "640 Shutter-IOChannel": "Dev1/ao14", "555 AO-Sequenceable": "1", "520 AO-Name": "AnalogIO", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "470 AO-Block voltage": "false", "COM1-Verbose": "1", "COM9-AnswerTimeout": "10000.0000", "528 Shutter-Name": "AnalogIO", "PRIME-Trigger Timeout (secs)": "10", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "NextFrame": 0, "470 AO-TriggerSequenceLength": "1000", "PRIME-TransposeXY": "0", "445 AO-TriggerSequenceLength": "1000", "COM1-Name": "COM1", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "COM9-DataBits": "8", "COM1-AnswerTimeout": "500.0000", "Camera": "", "TE2000-Version": "V2.50-V2.50-V2.10", "528 Shutter-Volts": "0.0000", "XLIGHT Hub-Name": "XLIGHT Hub", "PIEZO-Description": "Ludl stage driver adapter", "PositionName": "Pos34", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSampleRate": "100000", "PRIME-FanSpeedSetpoint": "High", "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "405 AO-TriggerSampleRate": "100000", "470 Shutter-Block voltage": "false", "470-State": "0", "COM3-Parity": "None", "405 AO-Volts": "0.0000", "PRIME-TransposeMirrorY": "0", "PRIME-ChipName": "GS144BSI", "470 AO-Volts": "0.0000", "PRIME-TransposeMirrorX": "0", "445 Shutter-SupportsTriggering": "0", "470 Shutter-SupportsTriggering": "0", "XLIGHT Hub-Description": "XLIGHT hub", "PRIME-PreampOffLimit": "10000", "470 AO-Sequenceable": "1", "528 Shutter-MaxVolts": "5.0000", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "Core-XYStage": "XYStage", "470 AO-TriggerSampleRate": "100000", "PRIME-Timing-PreTriggerDelayNs": "24672000.0000", "528-DA Device": "528 Shutter", "Focus-Description": "Nikon TE2000 focus adapter", "FilterBlock-State": "5", "520 AO-Demo": "No", "COM3-DelayBetweenCharsMs": "0.0000", "470 Shutter-Volts": "0.0000", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "PRIME-PP 2 THRESHOLD": "125", "PRIME-PP 4 THRESHOLD": "75", "XYStage-Acceleration": "4.0000", "470 Shutter-Demo": "No", "PRIME-Name": "PMPCIECam00", "640-DA Device": "640 Shutter", "405 AO-Block voltage": "false", "ElapsedTime-ms": 1406753, "PRIME-Offset": "100", "445 AO-IOChannel": "Dev1/ao1", "640 AO-MaxVolts": "5.0000", "PositionIndex": 34, "PRIME-PP 1 ENABLED": "Yes", "COM1-BaudRate": "9600", "640-State": "1", "COM3-Verbose": "1", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "LudlController-TransmissionDelay": "0", "PRIME-FullWellCapacity": "62000", "PRIME-SMARTStreamingEnabled": "No", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "640 Shutter-Description": "NI DAC", "528-Description": "DA device that is used as a shutter", "COM4-Description": "Serial port driver (boost:asio)", "405 Shutter-Description": "NI DAC", "TE2000-Name": "TE2000", "528 Shutter-Description": "NI DAC", "COM9-Description": "Serial port driver (boost:asio)", "LudlController-Name": "LudlController", "445 Shutter-TriggerSequenceLength": "1000", "PRIME-SerialNumber": "A18B203008", "445 AO-Volts": "0.0000", "PRIME-X-dimension": "1200", "PRIME-ShutterCloseDelay": "0", "XLIGHT Disk slider-State": "2", "Position": "Default", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 AO-IOChannel": "Dev1/ao5", "520 AO-IOChannel": "Dev1/ao4", "528 AO-TriggerSequenceLength": "1000", "405 Shutter-MaxVolts": "5.0000", "470-DA Device": "470 Shutter", "PRIME-FTCapable": "No", "Core-ChannelGroup": "Channels", "555 AO-SupportsTriggering": "0", "PRIME-Y-dimension": "1200", "Frame": 0, "555 Shutter-Sequenceable": "1", "PRIME-Timing-ExposureTimeNs": "499998000.0000", "Core-ImageProcessor": "", "528-State": "0", "520 AO-Block voltage": "false", "XLIGHT Excitation Wheel-Label": "QUAD", "640 Shutter-Demo": "No", "COM3-StopBits": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "520 Shutter-IOChannel": "Dev1/ao11", "Width": 1200, "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-SupportsTriggering": "0", "640 AO-TriggerSampleRate": "100000", "520-Description": "DA device that is used as a shutter", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "405 Shutter-Sequenceable": "1", "405 Shutter-SupportsTriggering": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-TriggerSampleRate": "100000", "640 Shutter-MaxVolts": "5.0000", "555 AO-MaxVolts": "5.0000", "520 AO-TriggerSequenceLength": "1000", "COM3-BaudRate": "9600", "COM9-BaudRate": "9600", "Vincent-D3-Channel #": "Ch. #1", "PRIME-PMode": "Normal", "OpticalPath-Label": "Left(100)", "BitDepth": 16, "555 AO-TriggerSequenceLength": "1000", "445 Shutter-Description": "NI DAC", "405 AO-Sequenceable": "1", "PRIME-AcquisitionMethod": "Callbacks", "555 AO-Name": "AnalogIO", "PRIME-ShutterMode": "Pre-Exposure", "COM3-Handshaking": "Off", "445 AO-SupportsTriggering": "0", "ROI": "0-0-1200-1200", "COM9-Verbose": "1", "PRIME-Color": "OFF", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "PRIME-ShutterOpenDelay": "0", "COM1-Parity": "None", "Slice": 6, "SlicePosition": 10, "COM9-DelayBetweenCharsMs": "0.0000", "555-Name": "DA Shutter", "PIEZO-LudlSingleAxisName": "B", "XLIGHT Emission Wheel-Label": "679/29 (A)", "640 Shutter-Name": "AnalogIO", "640 AO-Sequenceable": "1", "520 Shutter-Demo": "No", "528 Shutter-Demo": "No", "470-Name": "DA Shutter", "520-DA Device": "520 Shutter", "XYStage-Description": "Ludl XY stage driver adapter", "445 Shutter-MinVolts": "0.0000", "528 AO-TriggerSampleRate": "100000", "405 AO-MinVolts": "0.0000", "405 Shutter-TriggerSequenceLength": "1000", "Height": 1200, "555 AO-Block voltage": "false", "640 Shutter-Volts": "5.0000", "PixelType": "GRAY16", "445 Shutter-Block voltage": "false", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "520 Shutter-TriggerSequenceLength": "1000", "COM9-Name": "COM9", "Core-SLM": "", "555 AO-Volts": "0.0000", "555 Shutter-Block voltage": "false", "XYStage-Speed": "1800.0000", "520 AO-MaxVolts": "5.0000", "528 Shutter-SupportsTriggering": "0", "COM1-Description": "Serial port driver (boost:asio)", "PRIME-MetadataEnabled": "No", "520 Shutter-MinVolts": "0.0000", "470 Shutter-Sequenceable": "1", "PRIME-PreampDelay": "0", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-MinVolts": "0.0000", "ChannelIndex": 2, "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Core-AutoShutter": "1", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "Exposure-ms": 320, "PRIME-PP 2 ENABLED": "Yes", "640 Shutter-TriggerSampleRate": "100000", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "470 Shutter-IOChannel": "Dev1/ao10", "405 AO-Demo": "No", "PixelSizeUm": 1.1, "470 AO-Demo": "No", "470 Shutter-MaxVolts": "5.0000", "Vincent-D3-Name": "Vincent-D3", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FileName": "img_000000000_Il-A_006.tif", "LudlController-Reset": "Operate", "PRIME-Exposure": "320.00", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "445-Name": "DA Shutter", "555 Shutter-Name": "AnalogIO", "520 Shutter-TriggerSampleRate": "100000", "445 Shutter-IOChannel": "Dev1/ao9", "PRIME-TransposeCorrection": "0", "XYStage-StepSize": "0.1000", "445-Description": "DA device that is used as a shutter", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 AO-IOChannel": "Dev1/ao7", "520 Shutter-Name": "AnalogIO", "COM3-DataBits": "8", "640 AO-Volts": "4.5000", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "555 Shutter-IOChannel": "Dev1/ao13", "WaitInterval": null, "520 Shutter-Sequenceable": "1", "YPositionUm": 545.6, "445-State": "0", "445-DA Device": "445 Shutter", "PRIME-CircularBufferAutoSize": "ON", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "555 AO-TriggerSampleRate": "100000", "445 Shutter-Volts": "0.0000", "XLIGHT Hub-Port": "COM3", "Core-AutoFocus": "", "COM4-Handshaking": "Off", "528 Shutter-MinVolts": "0.0000", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "445 Shutter-Name": "AnalogIO", "528 Shutter-Sequenceable": "1", "XYStage-TransposeMirrorX": "0", "XYStage-TransposeMirrorY": "0", "PRIME-ReadoutRate": "100MHz 16bit", "XYStage-Name": "Stage", "640 AO-SupportsTriggering": "0", "640-Name": "DA Shutter", "640 Shutter-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "640 AO-Block voltage": "false", "445 Shutter-Sequenceable": "1", "470 AO-MaxVolts": "5.0000", "COM4-BaudRate": "9600", "470-Description": "DA device that is used as a shutter", "520 Shutter-Description": "NI DAC", "PRIME-CCDTemperature": "-14.8500", "Time": "2019-12-19 09:59:37 -0500", "640 AO-TriggerSequenceLength": "1000", "470 AO-Name": "AnalogIO", "XLIGHT Disk slider-Label": "Disk Pos 40um", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "Binning": "1x1", "TE2000-ModelType": "1", "COM3-AnswerTimeout": "500.0000", "555 Shutter-SupportsTriggering": "0", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "TE2000-Port": "COM4", "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Description": "NI DAC", "COM4-AnswerTimeout": "500.0000", "COM1-DelayBetweenCharsMs": "0.0000", "FilterBlock-Label": "Confocal", "445 AO-Name": "AnalogIO", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-Timing-ReadoutTimeNs": "24672000.0000", "528 AO-Demo": "No", "520 AO-Sequenceable": "1", "555-Description": "DA device that is used as a shutter", "405-DA Device": "405 Shutter", "405 AO-TriggerSequenceLength": "1000", "640 AO-MinVolts": "0.0000", "640 Shutter-Block voltage": "false", "FrameIndex": 0, "PRIME-Port": "CMOS", "555 AO-MinVolts": "0.0000", "COM3-Description": "Serial port driver (boost:asio)", "528 AO-Description": "NI DAC", "555 AO-IOChannel": "Dev1/ao6", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "555 AO-TriggerInputLine": "/Dev1/PFI0", "PRIME-ActualInterval-ms": "390.5200", "LudlController-Port": "COM9", "445 Shutter-Demo": "No", "PRIME-Trigger-Expose Out-Mux": "1", "SliceIndex": 6, "PRIME-CCDTemperatureSetPoint": "-15.0000", "405 Shutter-MinVolts": "0.0000", "PRIME-ExposeOutMode": "First Row", "AxisPositions": null, "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Volts": "0.0000", "528-Name": "DA Shutter", "555-State": "0", "XLIGHT Dichroic-Label": "Di03-R635-t1", "445 AO-Sequenceable": "1", "405-Name": "DA Shutter", "405 AO-IOChannel": "Dev1/ao0", "Core-Camera": "PRIME", "Focus-Name": "Focus", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "405 Shutter-IOChannel": "Dev1/ao8", "640 AO-Demo": "No", "640 Shutter-SupportsTriggering": "0", "445 AO-Demo": "No", "555-DA Device": "555 Shutter", "470 Shutter-Description": "NI DAC", "528 AO-Volts": "0.0000", "COM1-DataBits": "8", "Vincent-D3-Address": "x", "520 AO-SupportsTriggering": "0", "405-Description": "DA device that is used as a shutter", "640 Shutter-MinVolts": "0.0000", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "405 AO-Name": "AnalogIO", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "640 AO-TriggerInputLine": "/Dev1/PFI0", "405 AO-TriggerInputLine": "/Dev1/PFI0", "640-Description": "DA device that is used as a shutter", "445 Shutter-TriggerSampleRate": "100000", "470 Shutter-TriggerSequenceLength": "1000", "COM1-StopBits": "1", "520 Shutter-SupportsTriggering": "0", "405-State": "0", "Core-Initialize": "1", "XLIGHT Emission Wheel-State": "3", "520 AO-Description": "NI DAC", "528 AO-TriggerInputLine": "/Dev1/PFI0", "445 Shutter-MaxVolts": "5.0000", "COM9-Handshaking": "Off", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "XLIGHT Spin motor-Label": "On (spinning)", "PRIME-PP 5 ENABLED": "No", "UUID": "4380be2e-d186-453f-816a-ff5bd4d514bc", "PRIME-TriggerMode": "Internal Trigger", "COM1-Handshaking": "Off", "PRIME-Timing-PostTriggerDelayNs": "49344000.0000", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "Focus-Position": "17741.5000", "528 Shutter-Block voltage": "false", "Channel": "Il-A", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "555 Shutter-Demo": "No", "405 Shutter-Demo": "No", "528 AO-Name": "AnalogIO", "520-State": "0", "Core-Galvo": "", "405 Shutter-TriggerSampleRate": "100000", "Focus-FocusStepSize_(nm)": "100", "PRIME-PixelType": "16bit", "COM4-DataBits": "8", "470 AO-IOChannel": "Dev1/ao2", "520 AO-MinVolts": "0.0000", "COM9-Parity": "None", "PRIME-CircularBufferEnabled": "ON", "PRIME-PP 6 Reset": "No", "640 AO-Description": "NI DAC", "Source": "PRIME", "445 AO-MinVolts": "0.0000", "PIEZO-Name": "Stage", "445 AO-Description": "NI DAC", "PRIME-CircularBufferFrameCount": "46", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "520 AO-Volts": "0.0000", "528 Shutter-TriggerSampleRate": "100000", "XPositionUm": -1324.9, "OpticalPath-State": "4", "PRIME-PP 3 THRESHOLD": "80", "PRIME-PVCAM Version": "3.7.1", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Gain": "1-HDR", "640 Shutter-TriggerSequenceLength": "1000", "528 AO-SupportsTriggering": "0", "445 AO-TriggerInputLine": "/Dev1/PFI0", "OpticalPath-Name": "OpticalPath", "PRIME-PP 4 ENABLED": "Yes", "PRIME-PVCAM Adapter Version": "1.3.29", "COM3-Name": "COM3", "405 AO-MaxVolts": "5.0000", "520 Shutter-Block voltage": "false", "PRIME-Timing-ClearingTimeNs": "49344000.0000", "Core-Focus": "PIEZO", "528 AO-Block voltage": "false", "TE2000-Description": "Nikon TE2000 hub", "528 AO-MinVolts": "0.0000", "COM4-Parity": "None", "Summary": { "Slices": 11, "ChContrastMin": [ 0, 0, 0, 0, 0, 0 ], "PixelAspect": 1, "SlicesFirst": true, "Interval_ms": 0, "Time": "2019-12-19 09:59:21 -0500", "Prefix": "seq_13", "InitialPositionList": [ { "Label": "Pos0", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.21], "Focus": [17741.5], "XYStage": [ 44542.200000000004, 31636.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos1", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.22], "Focus": [17741.4], "XYStage": [ 43801.100000000006, 31637.9 ] }, "GridColumnIndex": 0 }, { "Label": "Pos2", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.5], "XYStage": [ 38561.3, 32040.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos3", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.185], "Focus": [17741.3], "XYStage": [ 37698.4, 32041.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos4", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.16], "Focus": [17741.5], "XYStage": [ 32375.4, 31758.9 ] }, "GridColumnIndex": 0 }, { "Label": "Pos5", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.17000000000002], "Focus": [17741.3], "XYStage": [ 31770.7, 31758.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos6", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.4], "XYStage": [ 26536.800000000003, 31068.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos7", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.205], "Focus": [17741.4], "XYStage": [ 25420.2, 31068.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos8", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.85], "Focus": [17741.3], "XYStage": [ 21059, 31508 ] }, "GridColumnIndex": 0 }, { "Label": "Pos9", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.82], "Focus": [17741.3], "XYStage": [ 19731.9, 31506.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos10", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.79], "Focus": [17741.4], "XYStage": [ 14528.5, 32007.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos11", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.83], "Focus": [17741.2], "XYStage": [ 13830.300000000001, 32009.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos12", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.79], "Focus": [17741.3], "XYStage": [ 8775.300000000001, 32017.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos13", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.815], "Focus": [17741.3], "XYStage": [ 8109.200000000001, 32008.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos14", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.805], "Focus": [17741.3], "XYStage": [ 3483.3, 31908.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos15", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.82], "Focus": [17741.2], "XYStage": [ 2686.8, 31903.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos16", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [211.48000000000002], "Focus": [17741.1], "XYStage": [ -1978.9, 31973.600000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos17", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [211.51500000000001], "Focus": [17741.1], "XYStage": [ -2957.8, 31982.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos18", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.195], "Focus": [17741.5], "XYStage": [ 47747.4, -576.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos19", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.23000000000002], "Focus": [17741.6], "XYStage": [ 47053.9, -576.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos20", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.205], "Focus": [17741.4], "XYStage": [ 41871.200000000004, -479.8 ] }, "GridColumnIndex": 0 }, { "Label": "Pos21", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.4], "XYStage": [ 40882.600000000006, -392 ] }, "GridColumnIndex": 0 }, { "Label": "Pos22", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.24], "Focus": [17741.4], "XYStage": [ 35905.6, -135.3 ] }, "GridColumnIndex": 0 }, { "Label": "Pos23", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.195], "Focus": [17741.5], "XYStage": [ 34990.9, -132.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos24", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.46], "Focus": [17741.4], "XYStage": [ 29678.100000000002, -958.1 ] }, "GridColumnIndex": 0 }, { "Label": "Pos25", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.44], "Focus": [17741.5], "XYStage": [ 28983.600000000002, -939.4000000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos26", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.475], "Focus": [17741.5], "XYStage": [ 23019.7, -122 ] }, "GridColumnIndex": 0 }, { "Label": "Pos27", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.44], "Focus": [17741.7], "XYStage": [ 22428, -116.80000000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos28", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.46], "Focus": [17741.7], "XYStage": [ 16364.900000000001, -181.10000000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos29", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.48000000000002], "Focus": [17741.5], "XYStage": [ 15712.1, -180 ] }, "GridColumnIndex": 0 }, { "Label": "Pos30", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.45000000000002], "Focus": [17741.7], "XYStage": [ 10346.1, 77.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos31", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.49], "Focus": [17741.4], "XYStage": [ 9755.5, 85.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos32", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.475], "Focus": [17741.5], "XYStage": [ 4775.5, 293.90000000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos33", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.445], "Focus": [17741.4], "XYStage": [ 3954.9, 293.90000000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos34", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.455], "Focus": [17741.4], "XYStage": [ -1324.9, 545.6 ] }, "GridColumnIndex": 0 }, { "Label": "Pos35", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.445], "Focus": [17741.3], "XYStage": [ -2097, 576.6 ] }, "GridColumnIndex": 0 } ], "z-step_um": 10, "GridRow": 0, "BitDepth": 16, "Depth": 2, "Channels": 6, "Source": "Micro-Manager", "ChColors": [ -16711681, -256, -65281, -1, -1, -16776961 ], "MetadataVersion": 10, "KeepShutterOpenChannels": true, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536, 65536 ], "CustomIntervals_ms": [], "Height": 1200, "MicroManagerVersion": "1.4.23 20180219", "GridColumn": 0, "UUID": "18808e7c-f2e6-47b3-90d8-1d779ce87dbd", "PixelType": "GRAY16", "Width": 1200, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\Yan\\20191218-YC113-S7S8-miseq", "Frames": 1, "Comment": "", "UserName": "Nikon", "PixelSize_um": 1.1, "ROI": [ 0, 0, 1200, 1200 ], "Date": "2019-12-19", "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC", "P-DAPI-high" ], "KeepShutterOpenSlices": true, "IJType": 1, "ComputerName": "ZadorConfocal2", "Positions": 36, "PositionIndex": 34, "CameraTimeout": 20000, "TimeFirst": false }, "640 AO-Name": "AnalogIO", "Vincent-D3-Command": "Close" }, "FrameKey-0-2-7": { "COM4-StopBits": "1", "528 AO-MaxVolts": "5.0000", "XLIGHT Excitation Wheel-State": "1", "470 Shutter-Name": "AnalogIO", "Core-Shutter": "640", "555 AO-Demo": "No", "XLIGHT Dichroic-State": "2", "XYStage-StepSize-Y": "0.1000", "ZPositionUm": 238.455, "XYStage-StepSize-X": "0.1000", "PRIME-FirmwareVersion": "20.28", "PRIME-ClearCycles": "2", "520-Name": "DA Shutter", "405 Shutter-Name": "AnalogIO", "PIEZO-StepSize": "0.0050", "PRIME-ClearMode": "Pre-Exposure", "470 AO-MinVolts": "0.0000", "405 AO-SupportsTriggering": "1", "CameraChannelIndex": 0, "XYStage-StartSpeed": "500.0000", "COM9-StopBits": "2", "405 AO-Description": "NI DAC", "COM4-DelayBetweenCharsMs": "0.0000", "445 AO-Block voltage": "false", "520 AO-TriggerInputLine": "/Dev1/PFI0", "555 AO-Description": "NI DAC", "520 AO-TriggerSampleRate": "100000", "COM4-Verbose": "1", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM4-Name": "COM4", "405 Shutter-Volts": "0.0000", "Vincent-D3-Port": "COM1", "PRIME-PP 4 MIN ADU AFFECTED": "200", "470 Shutter-MinVolts": "0.0000", "PRIME-PP 3 ENABLED": "Yes", "470 AO-Description": "NI DAC", "PRIME-Description": "PVCAM API device adapter", "520 Shutter-MaxVolts": "5.0000", "520 Shutter-Volts": "0.0000", "528 AO-Sequenceable": "1", "PRIME-Binning": "1x1", "555 Shutter-MaxVolts": "5.0000", "528 Shutter-TriggerSequenceLength": "1000", "405 Shutter-Block voltage": "false", "XLIGHT Spin motor-State": "1", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "Vincent-D3-Time to open (ms)": "35.0000", "Vincent-D3-Time to close (ms)": "35.0000", "445 AO-TriggerSampleRate": "100000", "640 Shutter-IOChannel": "Dev1/ao14", "555 AO-Sequenceable": "1", "520 AO-Name": "AnalogIO", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "470 AO-Block voltage": "false", "COM1-Verbose": "1", "COM9-AnswerTimeout": "10000.0000", "528 Shutter-Name": "AnalogIO", "PRIME-Trigger Timeout (secs)": "10", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "NextFrame": 0, "470 AO-TriggerSequenceLength": "1000", "PRIME-TransposeXY": "0", "445 AO-TriggerSequenceLength": "1000", "COM1-Name": "COM1", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "COM9-DataBits": "8", "COM1-AnswerTimeout": "500.0000", "Camera": "", "TE2000-Version": "V2.50-V2.50-V2.10", "528 Shutter-Volts": "0.0000", "XLIGHT Hub-Name": "XLIGHT Hub", "PIEZO-Description": "Ludl stage driver adapter", "PositionName": "Pos34", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSampleRate": "100000", "PRIME-FanSpeedSetpoint": "High", "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "405 AO-TriggerSampleRate": "100000", "470 Shutter-Block voltage": "false", "470-State": "0", "COM3-Parity": "None", "405 AO-Volts": "0.0000", "PRIME-TransposeMirrorY": "0", "PRIME-ChipName": "GS144BSI", "470 AO-Volts": "0.0000", "PRIME-TransposeMirrorX": "0", "445 Shutter-SupportsTriggering": "0", "470 Shutter-SupportsTriggering": "0", "XLIGHT Hub-Description": "XLIGHT hub", "PRIME-PreampOffLimit": "10000", "470 AO-Sequenceable": "1", "528 Shutter-MaxVolts": "5.0000", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "Core-XYStage": "XYStage", "470 AO-TriggerSampleRate": "100000", "PRIME-Timing-PreTriggerDelayNs": "24672000.0000", "528-DA Device": "528 Shutter", "Focus-Description": "Nikon TE2000 focus adapter", "FilterBlock-State": "5", "520 AO-Demo": "No", "COM3-DelayBetweenCharsMs": "0.0000", "470 Shutter-Volts": "0.0000", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "PRIME-PP 2 THRESHOLD": "125", "PRIME-PP 4 THRESHOLD": "75", "XYStage-Acceleration": "4.0000", "470 Shutter-Demo": "No", "PRIME-Name": "PMPCIECam00", "640-DA Device": "640 Shutter", "405 AO-Block voltage": "false", "ElapsedTime-ms": 1407370, "PRIME-Offset": "100", "445 AO-IOChannel": "Dev1/ao1", "640 AO-MaxVolts": "5.0000", "PositionIndex": 34, "PRIME-PP 1 ENABLED": "Yes", "COM1-BaudRate": "9600", "640-State": "1", "COM3-Verbose": "1", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "LudlController-TransmissionDelay": "0", "PRIME-FullWellCapacity": "62000", "PRIME-SMARTStreamingEnabled": "No", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "640 Shutter-Description": "NI DAC", "528-Description": "DA device that is used as a shutter", "COM4-Description": "Serial port driver (boost:asio)", "405 Shutter-Description": "NI DAC", "TE2000-Name": "TE2000", "528 Shutter-Description": "NI DAC", "COM9-Description": "Serial port driver (boost:asio)", "LudlController-Name": "LudlController", "445 Shutter-TriggerSequenceLength": "1000", "PRIME-SerialNumber": "A18B203008", "445 AO-Volts": "0.0000", "PRIME-X-dimension": "1200", "PRIME-ShutterCloseDelay": "0", "XLIGHT Disk slider-State": "2", "Position": "Default", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 AO-IOChannel": "Dev1/ao5", "520 AO-IOChannel": "Dev1/ao4", "528 AO-TriggerSequenceLength": "1000", "405 Shutter-MaxVolts": "5.0000", "470-DA Device": "470 Shutter", "PRIME-FTCapable": "No", "Core-ChannelGroup": "Channels", "555 AO-SupportsTriggering": "0", "PRIME-Y-dimension": "1200", "Frame": 0, "555 Shutter-Sequenceable": "1", "PRIME-Timing-ExposureTimeNs": "499998000.0000", "Core-ImageProcessor": "", "528-State": "0", "520 AO-Block voltage": "false", "XLIGHT Excitation Wheel-Label": "QUAD", "640 Shutter-Demo": "No", "COM3-StopBits": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "520 Shutter-IOChannel": "Dev1/ao11", "Width": 1200, "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-SupportsTriggering": "0", "640 AO-TriggerSampleRate": "100000", "520-Description": "DA device that is used as a shutter", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "405 Shutter-Sequenceable": "1", "405 Shutter-SupportsTriggering": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-TriggerSampleRate": "100000", "640 Shutter-MaxVolts": "5.0000", "555 AO-MaxVolts": "5.0000", "520 AO-TriggerSequenceLength": "1000", "COM3-BaudRate": "9600", "COM9-BaudRate": "9600", "Vincent-D3-Channel #": "Ch. #1", "PRIME-PMode": "Normal", "OpticalPath-Label": "Left(100)", "BitDepth": 16, "555 AO-TriggerSequenceLength": "1000", "445 Shutter-Description": "NI DAC", "405 AO-Sequenceable": "1", "PRIME-AcquisitionMethod": "Callbacks", "555 AO-Name": "AnalogIO", "PRIME-ShutterMode": "Pre-Exposure", "COM3-Handshaking": "Off", "445 AO-SupportsTriggering": "0", "ROI": "0-0-1200-1200", "COM9-Verbose": "1", "PRIME-Color": "OFF", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "PRIME-ShutterOpenDelay": "0", "COM1-Parity": "None", "Slice": 7, "SlicePosition": 20, "COM9-DelayBetweenCharsMs": "0.0000", "555-Name": "DA Shutter", "PIEZO-LudlSingleAxisName": "B", "XLIGHT Emission Wheel-Label": "679/29 (A)", "640 Shutter-Name": "AnalogIO", "640 AO-Sequenceable": "1", "520 Shutter-Demo": "No", "528 Shutter-Demo": "No", "470-Name": "DA Shutter", "520-DA Device": "520 Shutter", "XYStage-Description": "Ludl XY stage driver adapter", "445 Shutter-MinVolts": "0.0000", "528 AO-TriggerSampleRate": "100000", "405 AO-MinVolts": "0.0000", "405 Shutter-TriggerSequenceLength": "1000", "Height": 1200, "555 AO-Block voltage": "false", "640 Shutter-Volts": "5.0000", "PixelType": "GRAY16", "445 Shutter-Block voltage": "false", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "520 Shutter-TriggerSequenceLength": "1000", "COM9-Name": "COM9", "Core-SLM": "", "555 AO-Volts": "0.0000", "555 Shutter-Block voltage": "false", "XYStage-Speed": "1800.0000", "520 AO-MaxVolts": "5.0000", "528 Shutter-SupportsTriggering": "0", "COM1-Description": "Serial port driver (boost:asio)", "PRIME-MetadataEnabled": "No", "520 Shutter-MinVolts": "0.0000", "470 Shutter-Sequenceable": "1", "PRIME-PreampDelay": "0", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-MinVolts": "0.0000", "ChannelIndex": 2, "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Core-AutoShutter": "1", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "Exposure-ms": 320, "PRIME-PP 2 ENABLED": "Yes", "640 Shutter-TriggerSampleRate": "100000", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "470 Shutter-IOChannel": "Dev1/ao10", "405 AO-Demo": "No", "PixelSizeUm": 1.1, "470 AO-Demo": "No", "470 Shutter-MaxVolts": "5.0000", "Vincent-D3-Name": "Vincent-D3", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FileName": "img_000000000_Il-A_007.tif", "LudlController-Reset": "Operate", "PRIME-Exposure": "320.00", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "445-Name": "DA Shutter", "555 Shutter-Name": "AnalogIO", "520 Shutter-TriggerSampleRate": "100000", "445 Shutter-IOChannel": "Dev1/ao9", "PRIME-TransposeCorrection": "0", "XYStage-StepSize": "0.1000", "445-Description": "DA device that is used as a shutter", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 AO-IOChannel": "Dev1/ao7", "520 Shutter-Name": "AnalogIO", "COM3-DataBits": "8", "640 AO-Volts": "4.5000", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "555 Shutter-IOChannel": "Dev1/ao13", "WaitInterval": null, "520 Shutter-Sequenceable": "1", "YPositionUm": 545.6, "445-State": "0", "445-DA Device": "445 Shutter", "PRIME-CircularBufferAutoSize": "ON", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "555 AO-TriggerSampleRate": "100000", "445 Shutter-Volts": "0.0000", "XLIGHT Hub-Port": "COM3", "Core-AutoFocus": "", "COM4-Handshaking": "Off", "528 Shutter-MinVolts": "0.0000", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "445 Shutter-Name": "AnalogIO", "528 Shutter-Sequenceable": "1", "XYStage-TransposeMirrorX": "0", "XYStage-TransposeMirrorY": "0", "PRIME-ReadoutRate": "100MHz 16bit", "XYStage-Name": "Stage", "640 AO-SupportsTriggering": "0", "640-Name": "DA Shutter", "640 Shutter-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "640 AO-Block voltage": "false", "445 Shutter-Sequenceable": "1", "470 AO-MaxVolts": "5.0000", "COM4-BaudRate": "9600", "470-Description": "DA device that is used as a shutter", "520 Shutter-Description": "NI DAC", "PRIME-CCDTemperature": "-14.8500", "Time": "2019-12-19 09:59:37 -0500", "640 AO-TriggerSequenceLength": "1000", "470 AO-Name": "AnalogIO", "XLIGHT Disk slider-Label": "Disk Pos 40um", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "Binning": "1x1", "TE2000-ModelType": "1", "COM3-AnswerTimeout": "500.0000", "555 Shutter-SupportsTriggering": "0", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "TE2000-Port": "COM4", "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Description": "NI DAC", "COM4-AnswerTimeout": "500.0000", "COM1-DelayBetweenCharsMs": "0.0000", "FilterBlock-Label": "Confocal", "445 AO-Name": "AnalogIO", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-Timing-ReadoutTimeNs": "24672000.0000", "528 AO-Demo": "No", "520 AO-Sequenceable": "1", "555-Description": "DA device that is used as a shutter", "405-DA Device": "405 Shutter", "405 AO-TriggerSequenceLength": "1000", "640 AO-MinVolts": "0.0000", "640 Shutter-Block voltage": "false", "FrameIndex": 0, "PRIME-Port": "CMOS", "555 AO-MinVolts": "0.0000", "COM3-Description": "Serial port driver (boost:asio)", "528 AO-Description": "NI DAC", "555 AO-IOChannel": "Dev1/ao6", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "555 AO-TriggerInputLine": "/Dev1/PFI0", "PRIME-ActualInterval-ms": "390.5200", "LudlController-Port": "COM9", "445 Shutter-Demo": "No", "PRIME-Trigger-Expose Out-Mux": "1", "SliceIndex": 7, "PRIME-CCDTemperatureSetPoint": "-15.0000", "405 Shutter-MinVolts": "0.0000", "PRIME-ExposeOutMode": "First Row", "AxisPositions": null, "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Volts": "0.0000", "528-Name": "DA Shutter", "555-State": "0", "XLIGHT Dichroic-Label": "Di03-R635-t1", "445 AO-Sequenceable": "1", "405-Name": "DA Shutter", "405 AO-IOChannel": "Dev1/ao0", "Core-Camera": "PRIME", "Focus-Name": "Focus", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "405 Shutter-IOChannel": "Dev1/ao8", "640 AO-Demo": "No", "640 Shutter-SupportsTriggering": "0", "445 AO-Demo": "No", "555-DA Device": "555 Shutter", "470 Shutter-Description": "NI DAC", "528 AO-Volts": "0.0000", "COM1-DataBits": "8", "Vincent-D3-Address": "x", "520 AO-SupportsTriggering": "0", "405-Description": "DA device that is used as a shutter", "640 Shutter-MinVolts": "0.0000", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "405 AO-Name": "AnalogIO", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "640 AO-TriggerInputLine": "/Dev1/PFI0", "405 AO-TriggerInputLine": "/Dev1/PFI0", "640-Description": "DA device that is used as a shutter", "445 Shutter-TriggerSampleRate": "100000", "470 Shutter-TriggerSequenceLength": "1000", "COM1-StopBits": "1", "520 Shutter-SupportsTriggering": "0", "405-State": "0", "Core-Initialize": "1", "XLIGHT Emission Wheel-State": "3", "520 AO-Description": "NI DAC", "528 AO-TriggerInputLine": "/Dev1/PFI0", "445 Shutter-MaxVolts": "5.0000", "COM9-Handshaking": "Off", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "XLIGHT Spin motor-Label": "On (spinning)", "PRIME-PP 5 ENABLED": "No", "UUID": "eb9c4901-3480-4625-8cca-808efe5e5071", "PRIME-TriggerMode": "Internal Trigger", "COM1-Handshaking": "Off", "PRIME-Timing-PostTriggerDelayNs": "49344000.0000", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "Focus-Position": "17741.5000", "528 Shutter-Block voltage": "false", "Channel": "Il-A", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "555 Shutter-Demo": "No", "405 Shutter-Demo": "No", "528 AO-Name": "AnalogIO", "520-State": "0", "Core-Galvo": "", "405 Shutter-TriggerSampleRate": "100000", "Focus-FocusStepSize_(nm)": "100", "PRIME-PixelType": "16bit", "COM4-DataBits": "8", "470 AO-IOChannel": "Dev1/ao2", "520 AO-MinVolts": "0.0000", "COM9-Parity": "None", "PRIME-CircularBufferEnabled": "ON", "PRIME-PP 6 Reset": "No", "640 AO-Description": "NI DAC", "Source": "PRIME", "445 AO-MinVolts": "0.0000", "PIEZO-Name": "Stage", "445 AO-Description": "NI DAC", "PRIME-CircularBufferFrameCount": "46", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "520 AO-Volts": "0.0000", "528 Shutter-TriggerSampleRate": "100000", "XPositionUm": -1324.9, "OpticalPath-State": "4", "PRIME-PP 3 THRESHOLD": "80", "PRIME-PVCAM Version": "3.7.1", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Gain": "1-HDR", "640 Shutter-TriggerSequenceLength": "1000", "528 AO-SupportsTriggering": "0", "445 AO-TriggerInputLine": "/Dev1/PFI0", "OpticalPath-Name": "OpticalPath", "PRIME-PP 4 ENABLED": "Yes", "PRIME-PVCAM Adapter Version": "1.3.29", "COM3-Name": "COM3", "405 AO-MaxVolts": "5.0000", "520 Shutter-Block voltage": "false", "PRIME-Timing-ClearingTimeNs": "49344000.0000", "Core-Focus": "PIEZO", "528 AO-Block voltage": "false", "TE2000-Description": "Nikon TE2000 hub", "528 AO-MinVolts": "0.0000", "COM4-Parity": "None", "Summary": { "Slices": 11, "ChContrastMin": [ 0, 0, 0, 0, 0, 0 ], "PixelAspect": 1, "SlicesFirst": true, "Interval_ms": 0, "Time": "2019-12-19 09:59:21 -0500", "Prefix": "seq_13", "InitialPositionList": [ { "Label": "Pos0", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.21], "Focus": [17741.5], "XYStage": [ 44542.200000000004, 31636.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos1", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.22], "Focus": [17741.4], "XYStage": [ 43801.100000000006, 31637.9 ] }, "GridColumnIndex": 0 }, { "Label": "Pos2", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.5], "XYStage": [ 38561.3, 32040.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos3", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.185], "Focus": [17741.3], "XYStage": [ 37698.4, 32041.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos4", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.16], "Focus": [17741.5], "XYStage": [ 32375.4, 31758.9 ] }, "GridColumnIndex": 0 }, { "Label": "Pos5", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.17000000000002], "Focus": [17741.3], "XYStage": [ 31770.7, 31758.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos6", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.4], "XYStage": [ 26536.800000000003, 31068.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos7", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.205], "Focus": [17741.4], "XYStage": [ 25420.2, 31068.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos8", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.85], "Focus": [17741.3], "XYStage": [ 21059, 31508 ] }, "GridColumnIndex": 0 }, { "Label": "Pos9", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.82], "Focus": [17741.3], "XYStage": [ 19731.9, 31506.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos10", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.79], "Focus": [17741.4], "XYStage": [ 14528.5, 32007.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos11", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.83], "Focus": [17741.2], "XYStage": [ 13830.300000000001, 32009.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos12", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.79], "Focus": [17741.3], "XYStage": [ 8775.300000000001, 32017.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos13", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.815], "Focus": [17741.3], "XYStage": [ 8109.200000000001, 32008.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos14", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.805], "Focus": [17741.3], "XYStage": [ 3483.3, 31908.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos15", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.82], "Focus": [17741.2], "XYStage": [ 2686.8, 31903.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos16", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [211.48000000000002], "Focus": [17741.1], "XYStage": [ -1978.9, 31973.600000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos17", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [211.51500000000001], "Focus": [17741.1], "XYStage": [ -2957.8, 31982.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos18", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.195], "Focus": [17741.5], "XYStage": [ 47747.4, -576.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos19", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.23000000000002], "Focus": [17741.6], "XYStage": [ 47053.9, -576.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos20", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.205], "Focus": [17741.4], "XYStage": [ 41871.200000000004, -479.8 ] }, "GridColumnIndex": 0 }, { "Label": "Pos21", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.4], "XYStage": [ 40882.600000000006, -392 ] }, "GridColumnIndex": 0 }, { "Label": "Pos22", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.24], "Focus": [17741.4], "XYStage": [ 35905.6, -135.3 ] }, "GridColumnIndex": 0 }, { "Label": "Pos23", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.195], "Focus": [17741.5], "XYStage": [ 34990.9, -132.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos24", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.46], "Focus": [17741.4], "XYStage": [ 29678.100000000002, -958.1 ] }, "GridColumnIndex": 0 }, { "Label": "Pos25", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.44], "Focus": [17741.5], "XYStage": [ 28983.600000000002, -939.4000000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos26", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.475], "Focus": [17741.5], "XYStage": [ 23019.7, -122 ] }, "GridColumnIndex": 0 }, { "Label": "Pos27", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.44], "Focus": [17741.7], "XYStage": [ 22428, -116.80000000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos28", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.46], "Focus": [17741.7], "XYStage": [ 16364.900000000001, -181.10000000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos29", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.48000000000002], "Focus": [17741.5], "XYStage": [ 15712.1, -180 ] }, "GridColumnIndex": 0 }, { "Label": "Pos30", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.45000000000002], "Focus": [17741.7], "XYStage": [ 10346.1, 77.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos31", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.49], "Focus": [17741.4], "XYStage": [ 9755.5, 85.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos32", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.475], "Focus": [17741.5], "XYStage": [ 4775.5, 293.90000000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos33", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.445], "Focus": [17741.4], "XYStage": [ 3954.9, 293.90000000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos34", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.455], "Focus": [17741.4], "XYStage": [ -1324.9, 545.6 ] }, "GridColumnIndex": 0 }, { "Label": "Pos35", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.445], "Focus": [17741.3], "XYStage": [ -2097, 576.6 ] }, "GridColumnIndex": 0 } ], "z-step_um": 10, "GridRow": 0, "BitDepth": 16, "Depth": 2, "Channels": 6, "Source": "Micro-Manager", "ChColors": [ -16711681, -256, -65281, -1, -1, -16776961 ], "MetadataVersion": 10, "KeepShutterOpenChannels": true, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536, 65536 ], "CustomIntervals_ms": [], "Height": 1200, "MicroManagerVersion": "1.4.23 20180219", "GridColumn": 0, "UUID": "18808e7c-f2e6-47b3-90d8-1d779ce87dbd", "PixelType": "GRAY16", "Width": 1200, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\Yan\\20191218-YC113-S7S8-miseq", "Frames": 1, "Comment": "", "UserName": "Nikon", "PixelSize_um": 1.1, "ROI": [ 0, 0, 1200, 1200 ], "Date": "2019-12-19", "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC", "P-DAPI-high" ], "KeepShutterOpenSlices": true, "IJType": 1, "ComputerName": "ZadorConfocal2", "Positions": 36, "PositionIndex": 34, "CameraTimeout": 20000, "TimeFirst": false }, "640 AO-Name": "AnalogIO", "Vincent-D3-Command": "Close" }, "FrameKey-0-2-8": { "COM4-StopBits": "1", "528 AO-MaxVolts": "5.0000", "XLIGHT Excitation Wheel-State": "1", "470 Shutter-Name": "AnalogIO", "Core-Shutter": "640", "555 AO-Demo": "No", "XLIGHT Dichroic-State": "2", "XYStage-StepSize-Y": "0.1000", "ZPositionUm": 248.455, "XYStage-StepSize-X": "0.1000", "PRIME-FirmwareVersion": "20.28", "PRIME-ClearCycles": "2", "520-Name": "DA Shutter", "405 Shutter-Name": "AnalogIO", "PIEZO-StepSize": "0.0050", "PRIME-ClearMode": "Pre-Exposure", "470 AO-MinVolts": "0.0000", "405 AO-SupportsTriggering": "1", "CameraChannelIndex": 0, "XYStage-StartSpeed": "500.0000", "COM9-StopBits": "2", "405 AO-Description": "NI DAC", "COM4-DelayBetweenCharsMs": "0.0000", "445 AO-Block voltage": "false", "520 AO-TriggerInputLine": "/Dev1/PFI0", "555 AO-Description": "NI DAC", "520 AO-TriggerSampleRate": "100000", "COM4-Verbose": "1", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM4-Name": "COM4", "405 Shutter-Volts": "0.0000", "Vincent-D3-Port": "COM1", "PRIME-PP 4 MIN ADU AFFECTED": "200", "470 Shutter-MinVolts": "0.0000", "PRIME-PP 3 ENABLED": "Yes", "470 AO-Description": "NI DAC", "PRIME-Description": "PVCAM API device adapter", "520 Shutter-MaxVolts": "5.0000", "520 Shutter-Volts": "0.0000", "528 AO-Sequenceable": "1", "PRIME-Binning": "1x1", "555 Shutter-MaxVolts": "5.0000", "528 Shutter-TriggerSequenceLength": "1000", "405 Shutter-Block voltage": "false", "XLIGHT Spin motor-State": "1", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "Vincent-D3-Time to open (ms)": "35.0000", "Vincent-D3-Time to close (ms)": "35.0000", "445 AO-TriggerSampleRate": "100000", "640 Shutter-IOChannel": "Dev1/ao14", "555 AO-Sequenceable": "1", "520 AO-Name": "AnalogIO", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "470 AO-Block voltage": "false", "COM1-Verbose": "1", "COM9-AnswerTimeout": "10000.0000", "528 Shutter-Name": "AnalogIO", "PRIME-Trigger Timeout (secs)": "10", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "NextFrame": 0, "470 AO-TriggerSequenceLength": "1000", "PRIME-TransposeXY": "0", "445 AO-TriggerSequenceLength": "1000", "COM1-Name": "COM1", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "COM9-DataBits": "8", "COM1-AnswerTimeout": "500.0000", "Camera": "", "TE2000-Version": "V2.50-V2.50-V2.10", "528 Shutter-Volts": "0.0000", "XLIGHT Hub-Name": "XLIGHT Hub", "PIEZO-Description": "Ludl stage driver adapter", "PositionName": "Pos34", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSampleRate": "100000", "PRIME-FanSpeedSetpoint": "High", "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "405 AO-TriggerSampleRate": "100000", "470 Shutter-Block voltage": "false", "470-State": "0", "COM3-Parity": "None", "405 AO-Volts": "0.0000", "PRIME-TransposeMirrorY": "0", "PRIME-ChipName": "GS144BSI", "470 AO-Volts": "0.0000", "PRIME-TransposeMirrorX": "0", "445 Shutter-SupportsTriggering": "0", "470 Shutter-SupportsTriggering": "0", "XLIGHT Hub-Description": "XLIGHT hub", "PRIME-PreampOffLimit": "10000", "470 AO-Sequenceable": "1", "528 Shutter-MaxVolts": "5.0000", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "Core-XYStage": "XYStage", "470 AO-TriggerSampleRate": "100000", "PRIME-Timing-PreTriggerDelayNs": "24672000.0000", "528-DA Device": "528 Shutter", "Focus-Description": "Nikon TE2000 focus adapter", "FilterBlock-State": "5", "520 AO-Demo": "No", "COM3-DelayBetweenCharsMs": "0.0000", "470 Shutter-Volts": "0.0000", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "PRIME-PP 2 THRESHOLD": "125", "PRIME-PP 4 THRESHOLD": "75", "XYStage-Acceleration": "4.0000", "470 Shutter-Demo": "No", "PRIME-Name": "PMPCIECam00", "640-DA Device": "640 Shutter", "405 AO-Block voltage": "false", "ElapsedTime-ms": 1407939, "PRIME-Offset": "100", "445 AO-IOChannel": "Dev1/ao1", "640 AO-MaxVolts": "5.0000", "PositionIndex": 34, "PRIME-PP 1 ENABLED": "Yes", "COM1-BaudRate": "9600", "640-State": "1", "COM3-Verbose": "1", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "LudlController-TransmissionDelay": "0", "PRIME-FullWellCapacity": "62000", "PRIME-SMARTStreamingEnabled": "No", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "640 Shutter-Description": "NI DAC", "528-Description": "DA device that is used as a shutter", "COM4-Description": "Serial port driver (boost:asio)", "405 Shutter-Description": "NI DAC", "TE2000-Name": "TE2000", "528 Shutter-Description": "NI DAC", "COM9-Description": "Serial port driver (boost:asio)", "LudlController-Name": "LudlController", "445 Shutter-TriggerSequenceLength": "1000", "PRIME-SerialNumber": "A18B203008", "445 AO-Volts": "0.0000", "PRIME-X-dimension": "1200", "PRIME-ShutterCloseDelay": "0", "XLIGHT Disk slider-State": "2", "Position": "Default", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 AO-IOChannel": "Dev1/ao5", "520 AO-IOChannel": "Dev1/ao4", "528 AO-TriggerSequenceLength": "1000", "405 Shutter-MaxVolts": "5.0000", "470-DA Device": "470 Shutter", "PRIME-FTCapable": "No", "Core-ChannelGroup": "Channels", "555 AO-SupportsTriggering": "0", "PRIME-Y-dimension": "1200", "Frame": 0, "555 Shutter-Sequenceable": "1", "PRIME-Timing-ExposureTimeNs": "499998000.0000", "Core-ImageProcessor": "", "528-State": "0", "520 AO-Block voltage": "false", "XLIGHT Excitation Wheel-Label": "QUAD", "640 Shutter-Demo": "No", "COM3-StopBits": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "520 Shutter-IOChannel": "Dev1/ao11", "Width": 1200, "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-SupportsTriggering": "0", "640 AO-TriggerSampleRate": "100000", "520-Description": "DA device that is used as a shutter", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "405 Shutter-Sequenceable": "1", "405 Shutter-SupportsTriggering": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-TriggerSampleRate": "100000", "640 Shutter-MaxVolts": "5.0000", "555 AO-MaxVolts": "5.0000", "520 AO-TriggerSequenceLength": "1000", "COM3-BaudRate": "9600", "COM9-BaudRate": "9600", "Vincent-D3-Channel #": "Ch. #1", "PRIME-PMode": "Normal", "OpticalPath-Label": "Left(100)", "BitDepth": 16, "555 AO-TriggerSequenceLength": "1000", "445 Shutter-Description": "NI DAC", "405 AO-Sequenceable": "1", "PRIME-AcquisitionMethod": "Callbacks", "555 AO-Name": "AnalogIO", "PRIME-ShutterMode": "Pre-Exposure", "COM3-Handshaking": "Off", "445 AO-SupportsTriggering": "0", "ROI": "0-0-1200-1200", "COM9-Verbose": "1", "PRIME-Color": "OFF", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "PRIME-ShutterOpenDelay": "0", "COM1-Parity": "None", "Slice": 8, "SlicePosition": 30, "COM9-DelayBetweenCharsMs": "0.0000", "555-Name": "DA Shutter", "PIEZO-LudlSingleAxisName": "B", "XLIGHT Emission Wheel-Label": "679/29 (A)", "640 Shutter-Name": "AnalogIO", "640 AO-Sequenceable": "1", "520 Shutter-Demo": "No", "528 Shutter-Demo": "No", "470-Name": "DA Shutter", "520-DA Device": "520 Shutter", "XYStage-Description": "Ludl XY stage driver adapter", "445 Shutter-MinVolts": "0.0000", "528 AO-TriggerSampleRate": "100000", "405 AO-MinVolts": "0.0000", "405 Shutter-TriggerSequenceLength": "1000", "Height": 1200, "555 AO-Block voltage": "false", "640 Shutter-Volts": "5.0000", "PixelType": "GRAY16", "445 Shutter-Block voltage": "false", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "520 Shutter-TriggerSequenceLength": "1000", "COM9-Name": "COM9", "Core-SLM": "", "555 AO-Volts": "0.0000", "555 Shutter-Block voltage": "false", "XYStage-Speed": "1800.0000", "520 AO-MaxVolts": "5.0000", "528 Shutter-SupportsTriggering": "0", "COM1-Description": "Serial port driver (boost:asio)", "PRIME-MetadataEnabled": "No", "520 Shutter-MinVolts": "0.0000", "470 Shutter-Sequenceable": "1", "PRIME-PreampDelay": "0", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-MinVolts": "0.0000", "ChannelIndex": 2, "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Core-AutoShutter": "1", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "Exposure-ms": 320, "PRIME-PP 2 ENABLED": "Yes", "640 Shutter-TriggerSampleRate": "100000", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "470 Shutter-IOChannel": "Dev1/ao10", "405 AO-Demo": "No", "PixelSizeUm": 1.1, "470 AO-Demo": "No", "470 Shutter-MaxVolts": "5.0000", "Vincent-D3-Name": "Vincent-D3", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FileName": "img_000000000_Il-A_008.tif", "LudlController-Reset": "Operate", "PRIME-Exposure": "320.00", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "445-Name": "DA Shutter", "555 Shutter-Name": "AnalogIO", "520 Shutter-TriggerSampleRate": "100000", "445 Shutter-IOChannel": "Dev1/ao9", "PRIME-TransposeCorrection": "0", "XYStage-StepSize": "0.1000", "445-Description": "DA device that is used as a shutter", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 AO-IOChannel": "Dev1/ao7", "520 Shutter-Name": "AnalogIO", "COM3-DataBits": "8", "640 AO-Volts": "4.5000", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "555 Shutter-IOChannel": "Dev1/ao13", "WaitInterval": null, "520 Shutter-Sequenceable": "1", "YPositionUm": 545.6, "445-State": "0", "445-DA Device": "445 Shutter", "PRIME-CircularBufferAutoSize": "ON", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "555 AO-TriggerSampleRate": "100000", "445 Shutter-Volts": "0.0000", "XLIGHT Hub-Port": "COM3", "Core-AutoFocus": "", "COM4-Handshaking": "Off", "528 Shutter-MinVolts": "0.0000", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "445 Shutter-Name": "AnalogIO", "528 Shutter-Sequenceable": "1", "XYStage-TransposeMirrorX": "0", "XYStage-TransposeMirrorY": "0", "PRIME-ReadoutRate": "100MHz 16bit", "XYStage-Name": "Stage", "640 AO-SupportsTriggering": "0", "640-Name": "DA Shutter", "640 Shutter-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "640 AO-Block voltage": "false", "445 Shutter-Sequenceable": "1", "470 AO-MaxVolts": "5.0000", "COM4-BaudRate": "9600", "470-Description": "DA device that is used as a shutter", "520 Shutter-Description": "NI DAC", "PRIME-CCDTemperature": "-14.8500", "Time": "2019-12-19 09:59:38 -0500", "640 AO-TriggerSequenceLength": "1000", "470 AO-Name": "AnalogIO", "XLIGHT Disk slider-Label": "Disk Pos 40um", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "Binning": "1x1", "TE2000-ModelType": "1", "COM3-AnswerTimeout": "500.0000", "555 Shutter-SupportsTriggering": "0", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "TE2000-Port": "COM4", "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Description": "NI DAC", "COM4-AnswerTimeout": "500.0000", "COM1-DelayBetweenCharsMs": "0.0000", "FilterBlock-Label": "Confocal", "445 AO-Name": "AnalogIO", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-Timing-ReadoutTimeNs": "24672000.0000", "528 AO-Demo": "No", "520 AO-Sequenceable": "1", "555-Description": "DA device that is used as a shutter", "405-DA Device": "405 Shutter", "405 AO-TriggerSequenceLength": "1000", "640 AO-MinVolts": "0.0000", "640 Shutter-Block voltage": "false", "FrameIndex": 0, "PRIME-Port": "CMOS", "555 AO-MinVolts": "0.0000", "COM3-Description": "Serial port driver (boost:asio)", "528 AO-Description": "NI DAC", "555 AO-IOChannel": "Dev1/ao6", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "555 AO-TriggerInputLine": "/Dev1/PFI0", "PRIME-ActualInterval-ms": "390.5200", "LudlController-Port": "COM9", "445 Shutter-Demo": "No", "PRIME-Trigger-Expose Out-Mux": "1", "SliceIndex": 8, "PRIME-CCDTemperatureSetPoint": "-15.0000", "405 Shutter-MinVolts": "0.0000", "PRIME-ExposeOutMode": "First Row", "AxisPositions": null, "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Volts": "0.0000", "528-Name": "DA Shutter", "555-State": "0", "XLIGHT Dichroic-Label": "Di03-R635-t1", "445 AO-Sequenceable": "1", "405-Name": "DA Shutter", "405 AO-IOChannel": "Dev1/ao0", "Core-Camera": "PRIME", "Focus-Name": "Focus", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "405 Shutter-IOChannel": "Dev1/ao8", "640 AO-Demo": "No", "640 Shutter-SupportsTriggering": "0", "445 AO-Demo": "No", "555-DA Device": "555 Shutter", "470 Shutter-Description": "NI DAC", "528 AO-Volts": "0.0000", "COM1-DataBits": "8", "Vincent-D3-Address": "x", "520 AO-SupportsTriggering": "0", "405-Description": "DA device that is used as a shutter", "640 Shutter-MinVolts": "0.0000", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "405 AO-Name": "AnalogIO", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "640 AO-TriggerInputLine": "/Dev1/PFI0", "405 AO-TriggerInputLine": "/Dev1/PFI0", "640-Description": "DA device that is used as a shutter", "445 Shutter-TriggerSampleRate": "100000", "470 Shutter-TriggerSequenceLength": "1000", "COM1-StopBits": "1", "520 Shutter-SupportsTriggering": "0", "405-State": "0", "Core-Initialize": "1", "XLIGHT Emission Wheel-State": "3", "520 AO-Description": "NI DAC", "528 AO-TriggerInputLine": "/Dev1/PFI0", "445 Shutter-MaxVolts": "5.0000", "COM9-Handshaking": "Off", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "XLIGHT Spin motor-Label": "On (spinning)", "PRIME-PP 5 ENABLED": "No", "UUID": "adfcddb8-5607-4561-84f7-55a0f5a41f03", "PRIME-TriggerMode": "Internal Trigger", "COM1-Handshaking": "Off", "PRIME-Timing-PostTriggerDelayNs": "49344000.0000", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "Focus-Position": "17741.5000", "528 Shutter-Block voltage": "false", "Channel": "Il-A", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "555 Shutter-Demo": "No", "405 Shutter-Demo": "No", "528 AO-Name": "AnalogIO", "520-State": "0", "Core-Galvo": "", "405 Shutter-TriggerSampleRate": "100000", "Focus-FocusStepSize_(nm)": "100", "PRIME-PixelType": "16bit", "COM4-DataBits": "8", "470 AO-IOChannel": "Dev1/ao2", "520 AO-MinVolts": "0.0000", "COM9-Parity": "None", "PRIME-CircularBufferEnabled": "ON", "PRIME-PP 6 Reset": "No", "640 AO-Description": "NI DAC", "Source": "PRIME", "445 AO-MinVolts": "0.0000", "PIEZO-Name": "Stage", "445 AO-Description": "NI DAC", "PRIME-CircularBufferFrameCount": "46", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "520 AO-Volts": "0.0000", "528 Shutter-TriggerSampleRate": "100000", "XPositionUm": -1324.9, "OpticalPath-State": "4", "PRIME-PP 3 THRESHOLD": "80", "PRIME-PVCAM Version": "3.7.1", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Gain": "1-HDR", "640 Shutter-TriggerSequenceLength": "1000", "528 AO-SupportsTriggering": "0", "445 AO-TriggerInputLine": "/Dev1/PFI0", "OpticalPath-Name": "OpticalPath", "PRIME-PP 4 ENABLED": "Yes", "PRIME-PVCAM Adapter Version": "1.3.29", "COM3-Name": "COM3", "405 AO-MaxVolts": "5.0000", "520 Shutter-Block voltage": "false", "PRIME-Timing-ClearingTimeNs": "49344000.0000", "Core-Focus": "PIEZO", "528 AO-Block voltage": "false", "TE2000-Description": "Nikon TE2000 hub", "528 AO-MinVolts": "0.0000", "COM4-Parity": "None", "Summary": { "Slices": 11, "ChContrastMin": [ 0, 0, 0, 0, 0, 0 ], "PixelAspect": 1, "SlicesFirst": true, "Interval_ms": 0, "Time": "2019-12-19 09:59:21 -0500", "Prefix": "seq_13", "InitialPositionList": [ { "Label": "Pos0", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.21], "Focus": [17741.5], "XYStage": [ 44542.200000000004, 31636.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos1", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.22], "Focus": [17741.4], "XYStage": [ 43801.100000000006, 31637.9 ] }, "GridColumnIndex": 0 }, { "Label": "Pos2", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.5], "XYStage": [ 38561.3, 32040.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos3", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.185], "Focus": [17741.3], "XYStage": [ 37698.4, 32041.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos4", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.16], "Focus": [17741.5], "XYStage": [ 32375.4, 31758.9 ] }, "GridColumnIndex": 0 }, { "Label": "Pos5", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.17000000000002], "Focus": [17741.3], "XYStage": [ 31770.7, 31758.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos6", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.4], "XYStage": [ 26536.800000000003, 31068.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos7", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.205], "Focus": [17741.4], "XYStage": [ 25420.2, 31068.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos8", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.85], "Focus": [17741.3], "XYStage": [ 21059, 31508 ] }, "GridColumnIndex": 0 }, { "Label": "Pos9", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.82], "Focus": [17741.3], "XYStage": [ 19731.9, 31506.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos10", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.79], "Focus": [17741.4], "XYStage": [ 14528.5, 32007.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos11", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.83], "Focus": [17741.2], "XYStage": [ 13830.300000000001, 32009.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos12", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.79], "Focus": [17741.3], "XYStage": [ 8775.300000000001, 32017.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos13", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.815], "Focus": [17741.3], "XYStage": [ 8109.200000000001, 32008.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos14", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.805], "Focus": [17741.3], "XYStage": [ 3483.3, 31908.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos15", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.82], "Focus": [17741.2], "XYStage": [ 2686.8, 31903.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos16", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [211.48000000000002], "Focus": [17741.1], "XYStage": [ -1978.9, 31973.600000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos17", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [211.51500000000001], "Focus": [17741.1], "XYStage": [ -2957.8, 31982.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos18", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.195], "Focus": [17741.5], "XYStage": [ 47747.4, -576.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos19", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.23000000000002], "Focus": [17741.6], "XYStage": [ 47053.9, -576.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos20", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.205], "Focus": [17741.4], "XYStage": [ 41871.200000000004, -479.8 ] }, "GridColumnIndex": 0 }, { "Label": "Pos21", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.4], "XYStage": [ 40882.600000000006, -392 ] }, "GridColumnIndex": 0 }, { "Label": "Pos22", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.24], "Focus": [17741.4], "XYStage": [ 35905.6, -135.3 ] }, "GridColumnIndex": 0 }, { "Label": "Pos23", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.195], "Focus": [17741.5], "XYStage": [ 34990.9, -132.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos24", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.46], "Focus": [17741.4], "XYStage": [ 29678.100000000002, -958.1 ] }, "GridColumnIndex": 0 }, { "Label": "Pos25", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.44], "Focus": [17741.5], "XYStage": [ 28983.600000000002, -939.4000000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos26", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.475], "Focus": [17741.5], "XYStage": [ 23019.7, -122 ] }, "GridColumnIndex": 0 }, { "Label": "Pos27", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.44], "Focus": [17741.7], "XYStage": [ 22428, -116.80000000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos28", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.46], "Focus": [17741.7], "XYStage": [ 16364.900000000001, -181.10000000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos29", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.48000000000002], "Focus": [17741.5], "XYStage": [ 15712.1, -180 ] }, "GridColumnIndex": 0 }, { "Label": "Pos30", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.45000000000002], "Focus": [17741.7], "XYStage": [ 10346.1, 77.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos31", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.49], "Focus": [17741.4], "XYStage": [ 9755.5, 85.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos32", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.475], "Focus": [17741.5], "XYStage": [ 4775.5, 293.90000000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos33", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.445], "Focus": [17741.4], "XYStage": [ 3954.9, 293.90000000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos34", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.455], "Focus": [17741.4], "XYStage": [ -1324.9, 545.6 ] }, "GridColumnIndex": 0 }, { "Label": "Pos35", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.445], "Focus": [17741.3], "XYStage": [ -2097, 576.6 ] }, "GridColumnIndex": 0 } ], "z-step_um": 10, "GridRow": 0, "BitDepth": 16, "Depth": 2, "Channels": 6, "Source": "Micro-Manager", "ChColors": [ -16711681, -256, -65281, -1, -1, -16776961 ], "MetadataVersion": 10, "KeepShutterOpenChannels": true, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536, 65536 ], "CustomIntervals_ms": [], "Height": 1200, "MicroManagerVersion": "1.4.23 20180219", "GridColumn": 0, "UUID": "18808e7c-f2e6-47b3-90d8-1d779ce87dbd", "PixelType": "GRAY16", "Width": 1200, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\Yan\\20191218-YC113-S7S8-miseq", "Frames": 1, "Comment": "", "UserName": "Nikon", "PixelSize_um": 1.1, "ROI": [ 0, 0, 1200, 1200 ], "Date": "2019-12-19", "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC", "P-DAPI-high" ], "KeepShutterOpenSlices": true, "IJType": 1, "ComputerName": "ZadorConfocal2", "Positions": 36, "PositionIndex": 34, "CameraTimeout": 20000, "TimeFirst": false }, "640 AO-Name": "AnalogIO", "Vincent-D3-Command": "Close" }, "FrameKey-0-2-9": { "COM4-StopBits": "1", "528 AO-MaxVolts": "5.0000", "XLIGHT Excitation Wheel-State": "1", "470 Shutter-Name": "AnalogIO", "Core-Shutter": "640", "555 AO-Demo": "No", "XLIGHT Dichroic-State": "2", "XYStage-StepSize-Y": "0.1000", "ZPositionUm": 258.45500000000004, "XYStage-StepSize-X": "0.1000", "PRIME-FirmwareVersion": "20.28", "PRIME-ClearCycles": "2", "520-Name": "DA Shutter", "405 Shutter-Name": "AnalogIO", "PIEZO-StepSize": "0.0050", "PRIME-ClearMode": "Pre-Exposure", "470 AO-MinVolts": "0.0000", "405 AO-SupportsTriggering": "1", "CameraChannelIndex": 0, "XYStage-StartSpeed": "500.0000", "COM9-StopBits": "2", "405 AO-Description": "NI DAC", "COM4-DelayBetweenCharsMs": "0.0000", "445 AO-Block voltage": "false", "520 AO-TriggerInputLine": "/Dev1/PFI0", "555 AO-Description": "NI DAC", "520 AO-TriggerSampleRate": "100000", "COM4-Verbose": "1", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM4-Name": "COM4", "405 Shutter-Volts": "0.0000", "Vincent-D3-Port": "COM1", "PRIME-PP 4 MIN ADU AFFECTED": "200", "470 Shutter-MinVolts": "0.0000", "PRIME-PP 3 ENABLED": "Yes", "470 AO-Description": "NI DAC", "PRIME-Description": "PVCAM API device adapter", "520 Shutter-MaxVolts": "5.0000", "520 Shutter-Volts": "0.0000", "528 AO-Sequenceable": "1", "PRIME-Binning": "1x1", "555 Shutter-MaxVolts": "5.0000", "528 Shutter-TriggerSequenceLength": "1000", "405 Shutter-Block voltage": "false", "XLIGHT Spin motor-State": "1", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "Vincent-D3-Time to open (ms)": "35.0000", "Vincent-D3-Time to close (ms)": "35.0000", "445 AO-TriggerSampleRate": "100000", "640 Shutter-IOChannel": "Dev1/ao14", "555 AO-Sequenceable": "1", "520 AO-Name": "AnalogIO", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "470 AO-Block voltage": "false", "COM1-Verbose": "1", "COM9-AnswerTimeout": "10000.0000", "528 Shutter-Name": "AnalogIO", "PRIME-Trigger Timeout (secs)": "10", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "NextFrame": 0, "470 AO-TriggerSequenceLength": "1000", "PRIME-TransposeXY": "0", "445 AO-TriggerSequenceLength": "1000", "COM1-Name": "COM1", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "COM9-DataBits": "8", "COM1-AnswerTimeout": "500.0000", "Camera": "", "TE2000-Version": "V2.50-V2.50-V2.10", "528 Shutter-Volts": "0.0000", "XLIGHT Hub-Name": "XLIGHT Hub", "PIEZO-Description": "Ludl stage driver adapter", "PositionName": "Pos34", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSampleRate": "100000", "PRIME-FanSpeedSetpoint": "High", "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "405 AO-TriggerSampleRate": "100000", "470 Shutter-Block voltage": "false", "470-State": "0", "COM3-Parity": "None", "405 AO-Volts": "0.0000", "PRIME-TransposeMirrorY": "0", "PRIME-ChipName": "GS144BSI", "470 AO-Volts": "0.0000", "PRIME-TransposeMirrorX": "0", "445 Shutter-SupportsTriggering": "0", "470 Shutter-SupportsTriggering": "0", "XLIGHT Hub-Description": "XLIGHT hub", "PRIME-PreampOffLimit": "10000", "470 AO-Sequenceable": "1", "528 Shutter-MaxVolts": "5.0000", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "Core-XYStage": "XYStage", "470 AO-TriggerSampleRate": "100000", "PRIME-Timing-PreTriggerDelayNs": "24672000.0000", "528-DA Device": "528 Shutter", "Focus-Description": "Nikon TE2000 focus adapter", "FilterBlock-State": "5", "520 AO-Demo": "No", "COM3-DelayBetweenCharsMs": "0.0000", "470 Shutter-Volts": "0.0000", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "PRIME-PP 2 THRESHOLD": "125", "PRIME-PP 4 THRESHOLD": "75", "XYStage-Acceleration": "4.0000", "470 Shutter-Demo": "No", "PRIME-Name": "PMPCIECam00", "640-DA Device": "640 Shutter", "405 AO-Block voltage": "false", "ElapsedTime-ms": 1408542, "PRIME-Offset": "100", "445 AO-IOChannel": "Dev1/ao1", "640 AO-MaxVolts": "5.0000", "PositionIndex": 34, "PRIME-PP 1 ENABLED": "Yes", "COM1-BaudRate": "9600", "640-State": "1", "COM3-Verbose": "1", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "LudlController-TransmissionDelay": "0", "PRIME-FullWellCapacity": "62000", "PRIME-SMARTStreamingEnabled": "No", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "640 Shutter-Description": "NI DAC", "528-Description": "DA device that is used as a shutter", "COM4-Description": "Serial port driver (boost:asio)", "405 Shutter-Description": "NI DAC", "TE2000-Name": "TE2000", "528 Shutter-Description": "NI DAC", "COM9-Description": "Serial port driver (boost:asio)", "LudlController-Name": "LudlController", "445 Shutter-TriggerSequenceLength": "1000", "PRIME-SerialNumber": "A18B203008", "445 AO-Volts": "0.0000", "PRIME-X-dimension": "1200", "PRIME-ShutterCloseDelay": "0", "XLIGHT Disk slider-State": "2", "Position": "Default", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 AO-IOChannel": "Dev1/ao5", "520 AO-IOChannel": "Dev1/ao4", "528 AO-TriggerSequenceLength": "1000", "405 Shutter-MaxVolts": "5.0000", "470-DA Device": "470 Shutter", "PRIME-FTCapable": "No", "Core-ChannelGroup": "Channels", "555 AO-SupportsTriggering": "0", "PRIME-Y-dimension": "1200", "Frame": 0, "555 Shutter-Sequenceable": "1", "PRIME-Timing-ExposureTimeNs": "499998000.0000", "Core-ImageProcessor": "", "528-State": "0", "520 AO-Block voltage": "false", "XLIGHT Excitation Wheel-Label": "QUAD", "640 Shutter-Demo": "No", "COM3-StopBits": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "520 Shutter-IOChannel": "Dev1/ao11", "Width": 1200, "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-SupportsTriggering": "0", "640 AO-TriggerSampleRate": "100000", "520-Description": "DA device that is used as a shutter", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "405 Shutter-Sequenceable": "1", "405 Shutter-SupportsTriggering": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-TriggerSampleRate": "100000", "640 Shutter-MaxVolts": "5.0000", "555 AO-MaxVolts": "5.0000", "520 AO-TriggerSequenceLength": "1000", "COM3-BaudRate": "9600", "COM9-BaudRate": "9600", "Vincent-D3-Channel #": "Ch. #1", "PRIME-PMode": "Normal", "OpticalPath-Label": "Left(100)", "BitDepth": 16, "555 AO-TriggerSequenceLength": "1000", "445 Shutter-Description": "NI DAC", "405 AO-Sequenceable": "1", "PRIME-AcquisitionMethod": "Callbacks", "555 AO-Name": "AnalogIO", "PRIME-ShutterMode": "Pre-Exposure", "COM3-Handshaking": "Off", "445 AO-SupportsTriggering": "0", "ROI": "0-0-1200-1200", "COM9-Verbose": "1", "PRIME-Color": "OFF", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "PRIME-ShutterOpenDelay": "0", "COM1-Parity": "None", "Slice": 9, "SlicePosition": 40, "COM9-DelayBetweenCharsMs": "0.0000", "555-Name": "DA Shutter", "PIEZO-LudlSingleAxisName": "B", "XLIGHT Emission Wheel-Label": "679/29 (A)", "640 Shutter-Name": "AnalogIO", "640 AO-Sequenceable": "1", "520 Shutter-Demo": "No", "528 Shutter-Demo": "No", "470-Name": "DA Shutter", "520-DA Device": "520 Shutter", "XYStage-Description": "Ludl XY stage driver adapter", "445 Shutter-MinVolts": "0.0000", "528 AO-TriggerSampleRate": "100000", "405 AO-MinVolts": "0.0000", "405 Shutter-TriggerSequenceLength": "1000", "Height": 1200, "555 AO-Block voltage": "false", "640 Shutter-Volts": "5.0000", "PixelType": "GRAY16", "445 Shutter-Block voltage": "false", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "520 Shutter-TriggerSequenceLength": "1000", "COM9-Name": "COM9", "Core-SLM": "", "555 AO-Volts": "0.0000", "555 Shutter-Block voltage": "false", "XYStage-Speed": "1800.0000", "520 AO-MaxVolts": "5.0000", "528 Shutter-SupportsTriggering": "0", "COM1-Description": "Serial port driver (boost:asio)", "PRIME-MetadataEnabled": "No", "520 Shutter-MinVolts": "0.0000", "470 Shutter-Sequenceable": "1", "PRIME-PreampDelay": "0", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-MinVolts": "0.0000", "ChannelIndex": 2, "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Core-AutoShutter": "1", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "Exposure-ms": 320, "PRIME-PP 2 ENABLED": "Yes", "640 Shutter-TriggerSampleRate": "100000", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "470 Shutter-IOChannel": "Dev1/ao10", "405 AO-Demo": "No", "PixelSizeUm": 1.1, "470 AO-Demo": "No", "470 Shutter-MaxVolts": "5.0000", "Vincent-D3-Name": "Vincent-D3", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FileName": "img_000000000_Il-A_009.tif", "LudlController-Reset": "Operate", "PRIME-Exposure": "320.00", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "445-Name": "DA Shutter", "555 Shutter-Name": "AnalogIO", "520 Shutter-TriggerSampleRate": "100000", "445 Shutter-IOChannel": "Dev1/ao9", "PRIME-TransposeCorrection": "0", "XYStage-StepSize": "0.1000", "445-Description": "DA device that is used as a shutter", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 AO-IOChannel": "Dev1/ao7", "520 Shutter-Name": "AnalogIO", "COM3-DataBits": "8", "640 AO-Volts": "4.5000", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "555 Shutter-IOChannel": "Dev1/ao13", "WaitInterval": null, "520 Shutter-Sequenceable": "1", "YPositionUm": 545.6, "445-State": "0", "445-DA Device": "445 Shutter", "PRIME-CircularBufferAutoSize": "ON", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "555 AO-TriggerSampleRate": "100000", "445 Shutter-Volts": "0.0000", "XLIGHT Hub-Port": "COM3", "Core-AutoFocus": "", "COM4-Handshaking": "Off", "528 Shutter-MinVolts": "0.0000", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "445 Shutter-Name": "AnalogIO", "528 Shutter-Sequenceable": "1", "XYStage-TransposeMirrorX": "0", "XYStage-TransposeMirrorY": "0", "PRIME-ReadoutRate": "100MHz 16bit", "XYStage-Name": "Stage", "640 AO-SupportsTriggering": "0", "640-Name": "DA Shutter", "640 Shutter-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "640 AO-Block voltage": "false", "445 Shutter-Sequenceable": "1", "470 AO-MaxVolts": "5.0000", "COM4-BaudRate": "9600", "470-Description": "DA device that is used as a shutter", "520 Shutter-Description": "NI DAC", "PRIME-CCDTemperature": "-14.8500", "Time": "2019-12-19 09:59:38 -0500", "640 AO-TriggerSequenceLength": "1000", "470 AO-Name": "AnalogIO", "XLIGHT Disk slider-Label": "Disk Pos 40um", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "Binning": "1x1", "TE2000-ModelType": "1", "COM3-AnswerTimeout": "500.0000", "555 Shutter-SupportsTriggering": "0", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "TE2000-Port": "COM4", "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Description": "NI DAC", "COM4-AnswerTimeout": "500.0000", "COM1-DelayBetweenCharsMs": "0.0000", "FilterBlock-Label": "Confocal", "445 AO-Name": "AnalogIO", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-Timing-ReadoutTimeNs": "24672000.0000", "528 AO-Demo": "No", "520 AO-Sequenceable": "1", "555-Description": "DA device that is used as a shutter", "405-DA Device": "405 Shutter", "405 AO-TriggerSequenceLength": "1000", "640 AO-MinVolts": "0.0000", "640 Shutter-Block voltage": "false", "FrameIndex": 0, "PRIME-Port": "CMOS", "555 AO-MinVolts": "0.0000", "COM3-Description": "Serial port driver (boost:asio)", "528 AO-Description": "NI DAC", "555 AO-IOChannel": "Dev1/ao6", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "555 AO-TriggerInputLine": "/Dev1/PFI0", "PRIME-ActualInterval-ms": "390.5200", "LudlController-Port": "COM9", "445 Shutter-Demo": "No", "PRIME-Trigger-Expose Out-Mux": "1", "SliceIndex": 9, "PRIME-CCDTemperatureSetPoint": "-15.0000", "405 Shutter-MinVolts": "0.0000", "PRIME-ExposeOutMode": "First Row", "AxisPositions": null, "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Volts": "0.0000", "528-Name": "DA Shutter", "555-State": "0", "XLIGHT Dichroic-Label": "Di03-R635-t1", "445 AO-Sequenceable": "1", "405-Name": "DA Shutter", "405 AO-IOChannel": "Dev1/ao0", "Core-Camera": "PRIME", "Focus-Name": "Focus", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "405 Shutter-IOChannel": "Dev1/ao8", "640 AO-Demo": "No", "640 Shutter-SupportsTriggering": "0", "445 AO-Demo": "No", "555-DA Device": "555 Shutter", "470 Shutter-Description": "NI DAC", "528 AO-Volts": "0.0000", "COM1-DataBits": "8", "Vincent-D3-Address": "x", "520 AO-SupportsTriggering": "0", "405-Description": "DA device that is used as a shutter", "640 Shutter-MinVolts": "0.0000", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "405 AO-Name": "AnalogIO", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "640 AO-TriggerInputLine": "/Dev1/PFI0", "405 AO-TriggerInputLine": "/Dev1/PFI0", "640-Description": "DA device that is used as a shutter", "445 Shutter-TriggerSampleRate": "100000", "470 Shutter-TriggerSequenceLength": "1000", "COM1-StopBits": "1", "520 Shutter-SupportsTriggering": "0", "405-State": "0", "Core-Initialize": "1", "XLIGHT Emission Wheel-State": "3", "520 AO-Description": "NI DAC", "528 AO-TriggerInputLine": "/Dev1/PFI0", "445 Shutter-MaxVolts": "5.0000", "COM9-Handshaking": "Off", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "XLIGHT Spin motor-Label": "On (spinning)", "PRIME-PP 5 ENABLED": "No", "UUID": "4ccc87c6-c180-41f0-9817-a5f326ed5d5e", "PRIME-TriggerMode": "Internal Trigger", "COM1-Handshaking": "Off", "PRIME-Timing-PostTriggerDelayNs": "49344000.0000", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "Focus-Position": "17741.5000", "528 Shutter-Block voltage": "false", "Channel": "Il-A", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "555 Shutter-Demo": "No", "405 Shutter-Demo": "No", "528 AO-Name": "AnalogIO", "520-State": "0", "Core-Galvo": "", "405 Shutter-TriggerSampleRate": "100000", "Focus-FocusStepSize_(nm)": "100", "PRIME-PixelType": "16bit", "COM4-DataBits": "8", "470 AO-IOChannel": "Dev1/ao2", "520 AO-MinVolts": "0.0000", "COM9-Parity": "None", "PRIME-CircularBufferEnabled": "ON", "PRIME-PP 6 Reset": "No", "640 AO-Description": "NI DAC", "Source": "PRIME", "445 AO-MinVolts": "0.0000", "PIEZO-Name": "Stage", "445 AO-Description": "NI DAC", "PRIME-CircularBufferFrameCount": "46", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "520 AO-Volts": "0.0000", "528 Shutter-TriggerSampleRate": "100000", "XPositionUm": -1324.9, "OpticalPath-State": "4", "PRIME-PP 3 THRESHOLD": "80", "PRIME-PVCAM Version": "3.7.1", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Gain": "1-HDR", "640 Shutter-TriggerSequenceLength": "1000", "528 AO-SupportsTriggering": "0", "445 AO-TriggerInputLine": "/Dev1/PFI0", "OpticalPath-Name": "OpticalPath", "PRIME-PP 4 ENABLED": "Yes", "PRIME-PVCAM Adapter Version": "1.3.29", "COM3-Name": "COM3", "405 AO-MaxVolts": "5.0000", "520 Shutter-Block voltage": "false", "PRIME-Timing-ClearingTimeNs": "49344000.0000", "Core-Focus": "PIEZO", "528 AO-Block voltage": "false", "TE2000-Description": "Nikon TE2000 hub", "528 AO-MinVolts": "0.0000", "COM4-Parity": "None", "Summary": { "Slices": 11, "ChContrastMin": [ 0, 0, 0, 0, 0, 0 ], "PixelAspect": 1, "SlicesFirst": true, "Interval_ms": 0, "Time": "2019-12-19 09:59:21 -0500", "Prefix": "seq_13", "InitialPositionList": [ { "Label": "Pos0", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.21], "Focus": [17741.5], "XYStage": [ 44542.200000000004, 31636.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos1", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.22], "Focus": [17741.4], "XYStage": [ 43801.100000000006, 31637.9 ] }, "GridColumnIndex": 0 }, { "Label": "Pos2", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.5], "XYStage": [ 38561.3, 32040.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos3", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.185], "Focus": [17741.3], "XYStage": [ 37698.4, 32041.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos4", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.16], "Focus": [17741.5], "XYStage": [ 32375.4, 31758.9 ] }, "GridColumnIndex": 0 }, { "Label": "Pos5", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.17000000000002], "Focus": [17741.3], "XYStage": [ 31770.7, 31758.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos6", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.4], "XYStage": [ 26536.800000000003, 31068.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos7", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.205], "Focus": [17741.4], "XYStage": [ 25420.2, 31068.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos8", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.85], "Focus": [17741.3], "XYStage": [ 21059, 31508 ] }, "GridColumnIndex": 0 }, { "Label": "Pos9", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.82], "Focus": [17741.3], "XYStage": [ 19731.9, 31506.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos10", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.79], "Focus": [17741.4], "XYStage": [ 14528.5, 32007.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos11", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.83], "Focus": [17741.2], "XYStage": [ 13830.300000000001, 32009.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos12", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.79], "Focus": [17741.3], "XYStage": [ 8775.300000000001, 32017.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos13", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.815], "Focus": [17741.3], "XYStage": [ 8109.200000000001, 32008.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos14", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.805], "Focus": [17741.3], "XYStage": [ 3483.3, 31908.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos15", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.82], "Focus": [17741.2], "XYStage": [ 2686.8, 31903.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos16", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [211.48000000000002], "Focus": [17741.1], "XYStage": [ -1978.9, 31973.600000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos17", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [211.51500000000001], "Focus": [17741.1], "XYStage": [ -2957.8, 31982.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos18", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.195], "Focus": [17741.5], "XYStage": [ 47747.4, -576.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos19", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.23000000000002], "Focus": [17741.6], "XYStage": [ 47053.9, -576.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos20", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.205], "Focus": [17741.4], "XYStage": [ 41871.200000000004, -479.8 ] }, "GridColumnIndex": 0 }, { "Label": "Pos21", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.4], "XYStage": [ 40882.600000000006, -392 ] }, "GridColumnIndex": 0 }, { "Label": "Pos22", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.24], "Focus": [17741.4], "XYStage": [ 35905.6, -135.3 ] }, "GridColumnIndex": 0 }, { "Label": "Pos23", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.195], "Focus": [17741.5], "XYStage": [ 34990.9, -132.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos24", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.46], "Focus": [17741.4], "XYStage": [ 29678.100000000002, -958.1 ] }, "GridColumnIndex": 0 }, { "Label": "Pos25", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.44], "Focus": [17741.5], "XYStage": [ 28983.600000000002, -939.4000000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos26", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.475], "Focus": [17741.5], "XYStage": [ 23019.7, -122 ] }, "GridColumnIndex": 0 }, { "Label": "Pos27", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.44], "Focus": [17741.7], "XYStage": [ 22428, -116.80000000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos28", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.46], "Focus": [17741.7], "XYStage": [ 16364.900000000001, -181.10000000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos29", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.48000000000002], "Focus": [17741.5], "XYStage": [ 15712.1, -180 ] }, "GridColumnIndex": 0 }, { "Label": "Pos30", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.45000000000002], "Focus": [17741.7], "XYStage": [ 10346.1, 77.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos31", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.49], "Focus": [17741.4], "XYStage": [ 9755.5, 85.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos32", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.475], "Focus": [17741.5], "XYStage": [ 4775.5, 293.90000000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos33", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.445], "Focus": [17741.4], "XYStage": [ 3954.9, 293.90000000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos34", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.455], "Focus": [17741.4], "XYStage": [ -1324.9, 545.6 ] }, "GridColumnIndex": 0 }, { "Label": "Pos35", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.445], "Focus": [17741.3], "XYStage": [ -2097, 576.6 ] }, "GridColumnIndex": 0 } ], "z-step_um": 10, "GridRow": 0, "BitDepth": 16, "Depth": 2, "Channels": 6, "Source": "Micro-Manager", "ChColors": [ -16711681, -256, -65281, -1, -1, -16776961 ], "MetadataVersion": 10, "KeepShutterOpenChannels": true, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536, 65536 ], "CustomIntervals_ms": [], "Height": 1200, "MicroManagerVersion": "1.4.23 20180219", "GridColumn": 0, "UUID": "18808e7c-f2e6-47b3-90d8-1d779ce87dbd", "PixelType": "GRAY16", "Width": 1200, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\Yan\\20191218-YC113-S7S8-miseq", "Frames": 1, "Comment": "", "UserName": "Nikon", "PixelSize_um": 1.1, "ROI": [ 0, 0, 1200, 1200 ], "Date": "2019-12-19", "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC", "P-DAPI-high" ], "KeepShutterOpenSlices": true, "IJType": 1, "ComputerName": "ZadorConfocal2", "Positions": 36, "PositionIndex": 34, "CameraTimeout": 20000, "TimeFirst": false }, "640 AO-Name": "AnalogIO", "Vincent-D3-Command": "Close" }, "FrameKey-0-2-10": { "COM4-StopBits": "1", "528 AO-MaxVolts": "5.0000", "XLIGHT Excitation Wheel-State": "1", "470 Shutter-Name": "AnalogIO", "Core-Shutter": "640", "555 AO-Demo": "No", "XLIGHT Dichroic-State": "2", "XYStage-StepSize-Y": "0.1000", "ZPositionUm": 268.45500000000004, "XYStage-StepSize-X": "0.1000", "PRIME-FirmwareVersion": "20.28", "PRIME-ClearCycles": "2", "520-Name": "DA Shutter", "405 Shutter-Name": "AnalogIO", "PIEZO-StepSize": "0.0050", "PRIME-ClearMode": "Pre-Exposure", "470 AO-MinVolts": "0.0000", "405 AO-SupportsTriggering": "1", "CameraChannelIndex": 0, "XYStage-StartSpeed": "500.0000", "COM9-StopBits": "2", "405 AO-Description": "NI DAC", "COM4-DelayBetweenCharsMs": "0.0000", "445 AO-Block voltage": "false", "520 AO-TriggerInputLine": "/Dev1/PFI0", "555 AO-Description": "NI DAC", "520 AO-TriggerSampleRate": "100000", "COM4-Verbose": "1", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM4-Name": "COM4", "405 Shutter-Volts": "0.0000", "Vincent-D3-Port": "COM1", "PRIME-PP 4 MIN ADU AFFECTED": "200", "470 Shutter-MinVolts": "0.0000", "PRIME-PP 3 ENABLED": "Yes", "470 AO-Description": "NI DAC", "PRIME-Description": "PVCAM API device adapter", "520 Shutter-MaxVolts": "5.0000", "520 Shutter-Volts": "0.0000", "528 AO-Sequenceable": "1", "PRIME-Binning": "1x1", "555 Shutter-MaxVolts": "5.0000", "528 Shutter-TriggerSequenceLength": "1000", "405 Shutter-Block voltage": "false", "XLIGHT Spin motor-State": "1", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "Vincent-D3-Time to open (ms)": "35.0000", "Vincent-D3-Time to close (ms)": "35.0000", "445 AO-TriggerSampleRate": "100000", "640 Shutter-IOChannel": "Dev1/ao14", "555 AO-Sequenceable": "1", "520 AO-Name": "AnalogIO", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "470 AO-Block voltage": "false", "COM1-Verbose": "1", "COM9-AnswerTimeout": "10000.0000", "528 Shutter-Name": "AnalogIO", "PRIME-Trigger Timeout (secs)": "10", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "NextFrame": 0, "470 AO-TriggerSequenceLength": "1000", "PRIME-TransposeXY": "0", "445 AO-TriggerSequenceLength": "1000", "COM1-Name": "COM1", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "COM9-DataBits": "8", "COM1-AnswerTimeout": "500.0000", "Camera": "", "TE2000-Version": "V2.50-V2.50-V2.10", "528 Shutter-Volts": "0.0000", "XLIGHT Hub-Name": "XLIGHT Hub", "PIEZO-Description": "Ludl stage driver adapter", "PositionName": "Pos34", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSampleRate": "100000", "PRIME-FanSpeedSetpoint": "High", "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "405 AO-TriggerSampleRate": "100000", "470 Shutter-Block voltage": "false", "470-State": "0", "COM3-Parity": "None", "405 AO-Volts": "0.0000", "PRIME-TransposeMirrorY": "0", "PRIME-ChipName": "GS144BSI", "470 AO-Volts": "0.0000", "PRIME-TransposeMirrorX": "0", "445 Shutter-SupportsTriggering": "0", "470 Shutter-SupportsTriggering": "0", "XLIGHT Hub-Description": "XLIGHT hub", "PRIME-PreampOffLimit": "10000", "470 AO-Sequenceable": "1", "528 Shutter-MaxVolts": "5.0000", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "Core-XYStage": "XYStage", "470 AO-TriggerSampleRate": "100000", "PRIME-Timing-PreTriggerDelayNs": "24672000.0000", "528-DA Device": "528 Shutter", "Focus-Description": "Nikon TE2000 focus adapter", "FilterBlock-State": "5", "520 AO-Demo": "No", "COM3-DelayBetweenCharsMs": "0.0000", "470 Shutter-Volts": "0.0000", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "PRIME-PP 2 THRESHOLD": "125", "PRIME-PP 4 THRESHOLD": "75", "XYStage-Acceleration": "4.0000", "470 Shutter-Demo": "No", "PRIME-Name": "PMPCIECam00", "640-DA Device": "640 Shutter", "405 AO-Block voltage": "false", "ElapsedTime-ms": 1409106, "PRIME-Offset": "100", "445 AO-IOChannel": "Dev1/ao1", "640 AO-MaxVolts": "5.0000", "PositionIndex": 34, "PRIME-PP 1 ENABLED": "Yes", "COM1-BaudRate": "9600", "640-State": "1", "COM3-Verbose": "1", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "LudlController-TransmissionDelay": "0", "PRIME-FullWellCapacity": "62000", "PRIME-SMARTStreamingEnabled": "No", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "640 Shutter-Description": "NI DAC", "528-Description": "DA device that is used as a shutter", "COM4-Description": "Serial port driver (boost:asio)", "405 Shutter-Description": "NI DAC", "TE2000-Name": "TE2000", "528 Shutter-Description": "NI DAC", "COM9-Description": "Serial port driver (boost:asio)", "LudlController-Name": "LudlController", "445 Shutter-TriggerSequenceLength": "1000", "PRIME-SerialNumber": "A18B203008", "445 AO-Volts": "0.0000", "PRIME-X-dimension": "1200", "PRIME-ShutterCloseDelay": "0", "XLIGHT Disk slider-State": "2", "Position": "Default", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 AO-IOChannel": "Dev1/ao5", "520 AO-IOChannel": "Dev1/ao4", "528 AO-TriggerSequenceLength": "1000", "405 Shutter-MaxVolts": "5.0000", "470-DA Device": "470 Shutter", "PRIME-FTCapable": "No", "Core-ChannelGroup": "Channels", "555 AO-SupportsTriggering": "0", "PRIME-Y-dimension": "1200", "Frame": 0, "555 Shutter-Sequenceable": "1", "PRIME-Timing-ExposureTimeNs": "499998000.0000", "Core-ImageProcessor": "", "528-State": "0", "520 AO-Block voltage": "false", "XLIGHT Excitation Wheel-Label": "QUAD", "640 Shutter-Demo": "No", "COM3-StopBits": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "520 Shutter-IOChannel": "Dev1/ao11", "Width": 1200, "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-SupportsTriggering": "0", "640 AO-TriggerSampleRate": "100000", "520-Description": "DA device that is used as a shutter", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "405 Shutter-Sequenceable": "1", "405 Shutter-SupportsTriggering": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-TriggerSampleRate": "100000", "640 Shutter-MaxVolts": "5.0000", "555 AO-MaxVolts": "5.0000", "520 AO-TriggerSequenceLength": "1000", "COM3-BaudRate": "9600", "COM9-BaudRate": "9600", "Vincent-D3-Channel #": "Ch. #1", "PRIME-PMode": "Normal", "OpticalPath-Label": "Left(100)", "BitDepth": 16, "555 AO-TriggerSequenceLength": "1000", "445 Shutter-Description": "NI DAC", "405 AO-Sequenceable": "1", "PRIME-AcquisitionMethod": "Callbacks", "555 AO-Name": "AnalogIO", "PRIME-ShutterMode": "Pre-Exposure", "COM3-Handshaking": "Off", "445 AO-SupportsTriggering": "0", "ROI": "0-0-1200-1200", "COM9-Verbose": "1", "PRIME-Color": "OFF", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "PRIME-ShutterOpenDelay": "0", "COM1-Parity": "None", "Slice": 10, "SlicePosition": 50, "COM9-DelayBetweenCharsMs": "0.0000", "555-Name": "DA Shutter", "PIEZO-LudlSingleAxisName": "B", "XLIGHT Emission Wheel-Label": "679/29 (A)", "640 Shutter-Name": "AnalogIO", "640 AO-Sequenceable": "1", "520 Shutter-Demo": "No", "528 Shutter-Demo": "No", "470-Name": "DA Shutter", "520-DA Device": "520 Shutter", "XYStage-Description": "Ludl XY stage driver adapter", "445 Shutter-MinVolts": "0.0000", "528 AO-TriggerSampleRate": "100000", "405 AO-MinVolts": "0.0000", "405 Shutter-TriggerSequenceLength": "1000", "Height": 1200, "555 AO-Block voltage": "false", "640 Shutter-Volts": "5.0000", "PixelType": "GRAY16", "445 Shutter-Block voltage": "false", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "520 Shutter-TriggerSequenceLength": "1000", "COM9-Name": "COM9", "Core-SLM": "", "555 AO-Volts": "0.0000", "555 Shutter-Block voltage": "false", "XYStage-Speed": "1800.0000", "520 AO-MaxVolts": "5.0000", "528 Shutter-SupportsTriggering": "0", "COM1-Description": "Serial port driver (boost:asio)", "PRIME-MetadataEnabled": "No", "520 Shutter-MinVolts": "0.0000", "470 Shutter-Sequenceable": "1", "PRIME-PreampDelay": "0", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-MinVolts": "0.0000", "ChannelIndex": 2, "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Core-AutoShutter": "1", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "Exposure-ms": 320, "PRIME-PP 2 ENABLED": "Yes", "640 Shutter-TriggerSampleRate": "100000", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "470 Shutter-IOChannel": "Dev1/ao10", "405 AO-Demo": "No", "PixelSizeUm": 1.1, "470 AO-Demo": "No", "470 Shutter-MaxVolts": "5.0000", "Vincent-D3-Name": "Vincent-D3", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FileName": "img_000000000_Il-A_010.tif", "LudlController-Reset": "Operate", "PRIME-Exposure": "320.00", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "445-Name": "DA Shutter", "555 Shutter-Name": "AnalogIO", "520 Shutter-TriggerSampleRate": "100000", "445 Shutter-IOChannel": "Dev1/ao9", "PRIME-TransposeCorrection": "0", "XYStage-StepSize": "0.1000", "445-Description": "DA device that is used as a shutter", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 AO-IOChannel": "Dev1/ao7", "520 Shutter-Name": "AnalogIO", "COM3-DataBits": "8", "640 AO-Volts": "4.5000", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "555 Shutter-IOChannel": "Dev1/ao13", "WaitInterval": null, "520 Shutter-Sequenceable": "1", "YPositionUm": 545.6, "445-State": "0", "445-DA Device": "445 Shutter", "PRIME-CircularBufferAutoSize": "ON", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "555 AO-TriggerSampleRate": "100000", "445 Shutter-Volts": "0.0000", "XLIGHT Hub-Port": "COM3", "Core-AutoFocus": "", "COM4-Handshaking": "Off", "528 Shutter-MinVolts": "0.0000", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "445 Shutter-Name": "AnalogIO", "528 Shutter-Sequenceable": "1", "XYStage-TransposeMirrorX": "0", "XYStage-TransposeMirrorY": "0", "PRIME-ReadoutRate": "100MHz 16bit", "XYStage-Name": "Stage", "640 AO-SupportsTriggering": "0", "640-Name": "DA Shutter", "640 Shutter-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "640 AO-Block voltage": "false", "445 Shutter-Sequenceable": "1", "470 AO-MaxVolts": "5.0000", "COM4-BaudRate": "9600", "470-Description": "DA device that is used as a shutter", "520 Shutter-Description": "NI DAC", "PRIME-CCDTemperature": "-14.8500", "Time": "2019-12-19 09:59:39 -0500", "640 AO-TriggerSequenceLength": "1000", "470 AO-Name": "AnalogIO", "XLIGHT Disk slider-Label": "Disk Pos 40um", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "Binning": "1x1", "TE2000-ModelType": "1", "COM3-AnswerTimeout": "500.0000", "555 Shutter-SupportsTriggering": "0", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "TE2000-Port": "COM4", "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Description": "NI DAC", "COM4-AnswerTimeout": "500.0000", "COM1-DelayBetweenCharsMs": "0.0000", "FilterBlock-Label": "Confocal", "445 AO-Name": "AnalogIO", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-Timing-ReadoutTimeNs": "24672000.0000", "528 AO-Demo": "No", "520 AO-Sequenceable": "1", "555-Description": "DA device that is used as a shutter", "405-DA Device": "405 Shutter", "405 AO-TriggerSequenceLength": "1000", "640 AO-MinVolts": "0.0000", "640 Shutter-Block voltage": "false", "FrameIndex": 0, "PRIME-Port": "CMOS", "555 AO-MinVolts": "0.0000", "COM3-Description": "Serial port driver (boost:asio)", "528 AO-Description": "NI DAC", "555 AO-IOChannel": "Dev1/ao6", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "555 AO-TriggerInputLine": "/Dev1/PFI0", "PRIME-ActualInterval-ms": "390.5200", "LudlController-Port": "COM9", "445 Shutter-Demo": "No", "PRIME-Trigger-Expose Out-Mux": "1", "SliceIndex": 10, "PRIME-CCDTemperatureSetPoint": "-15.0000", "405 Shutter-MinVolts": "0.0000", "PRIME-ExposeOutMode": "First Row", "AxisPositions": null, "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Volts": "0.0000", "528-Name": "DA Shutter", "555-State": "0", "XLIGHT Dichroic-Label": "Di03-R635-t1", "445 AO-Sequenceable": "1", "405-Name": "DA Shutter", "405 AO-IOChannel": "Dev1/ao0", "Core-Camera": "PRIME", "Focus-Name": "Focus", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "405 Shutter-IOChannel": "Dev1/ao8", "640 AO-Demo": "No", "640 Shutter-SupportsTriggering": "0", "445 AO-Demo": "No", "555-DA Device": "555 Shutter", "470 Shutter-Description": "NI DAC", "528 AO-Volts": "0.0000", "COM1-DataBits": "8", "Vincent-D3-Address": "x", "520 AO-SupportsTriggering": "0", "405-Description": "DA device that is used as a shutter", "640 Shutter-MinVolts": "0.0000", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "405 AO-Name": "AnalogIO", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "640 AO-TriggerInputLine": "/Dev1/PFI0", "405 AO-TriggerInputLine": "/Dev1/PFI0", "640-Description": "DA device that is used as a shutter", "445 Shutter-TriggerSampleRate": "100000", "470 Shutter-TriggerSequenceLength": "1000", "COM1-StopBits": "1", "520 Shutter-SupportsTriggering": "0", "405-State": "0", "Core-Initialize": "1", "XLIGHT Emission Wheel-State": "3", "520 AO-Description": "NI DAC", "528 AO-TriggerInputLine": "/Dev1/PFI0", "445 Shutter-MaxVolts": "5.0000", "COM9-Handshaking": "Off", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "XLIGHT Spin motor-Label": "On (spinning)", "PRIME-PP 5 ENABLED": "No", "UUID": "605f0037-6780-472d-9482-5a8f2c8e2fcd", "PRIME-TriggerMode": "Internal Trigger", "COM1-Handshaking": "Off", "PRIME-Timing-PostTriggerDelayNs": "49344000.0000", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "Focus-Position": "17741.5000", "528 Shutter-Block voltage": "false", "Channel": "Il-A", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "555 Shutter-Demo": "No", "405 Shutter-Demo": "No", "528 AO-Name": "AnalogIO", "520-State": "0", "Core-Galvo": "", "405 Shutter-TriggerSampleRate": "100000", "Focus-FocusStepSize_(nm)": "100", "PRIME-PixelType": "16bit", "COM4-DataBits": "8", "470 AO-IOChannel": "Dev1/ao2", "520 AO-MinVolts": "0.0000", "COM9-Parity": "None", "PRIME-CircularBufferEnabled": "ON", "PRIME-PP 6 Reset": "No", "640 AO-Description": "NI DAC", "Source": "PRIME", "445 AO-MinVolts": "0.0000", "PIEZO-Name": "Stage", "445 AO-Description": "NI DAC", "PRIME-CircularBufferFrameCount": "46", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "520 AO-Volts": "0.0000", "528 Shutter-TriggerSampleRate": "100000", "XPositionUm": -1324.9, "OpticalPath-State": "4", "PRIME-PP 3 THRESHOLD": "80", "PRIME-PVCAM Version": "3.7.1", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Gain": "1-HDR", "640 Shutter-TriggerSequenceLength": "1000", "528 AO-SupportsTriggering": "0", "445 AO-TriggerInputLine": "/Dev1/PFI0", "OpticalPath-Name": "OpticalPath", "PRIME-PP 4 ENABLED": "Yes", "PRIME-PVCAM Adapter Version": "1.3.29", "COM3-Name": "COM3", "405 AO-MaxVolts": "5.0000", "520 Shutter-Block voltage": "false", "PRIME-Timing-ClearingTimeNs": "49344000.0000", "Core-Focus": "PIEZO", "528 AO-Block voltage": "false", "TE2000-Description": "Nikon TE2000 hub", "528 AO-MinVolts": "0.0000", "COM4-Parity": "None", "Summary": { "Slices": 11, "ChContrastMin": [ 0, 0, 0, 0, 0, 0 ], "PixelAspect": 1, "SlicesFirst": true, "Interval_ms": 0, "Time": "2019-12-19 09:59:21 -0500", "Prefix": "seq_13", "InitialPositionList": [ { "Label": "Pos0", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.21], "Focus": [17741.5], "XYStage": [ 44542.200000000004, 31636.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos1", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.22], "Focus": [17741.4], "XYStage": [ 43801.100000000006, 31637.9 ] }, "GridColumnIndex": 0 }, { "Label": "Pos2", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.5], "XYStage": [ 38561.3, 32040.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos3", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.185], "Focus": [17741.3], "XYStage": [ 37698.4, 32041.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos4", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.16], "Focus": [17741.5], "XYStage": [ 32375.4, 31758.9 ] }, "GridColumnIndex": 0 }, { "Label": "Pos5", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.17000000000002], "Focus": [17741.3], "XYStage": [ 31770.7, 31758.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos6", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.4], "XYStage": [ 26536.800000000003, 31068.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos7", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.205], "Focus": [17741.4], "XYStage": [ 25420.2, 31068.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos8", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.85], "Focus": [17741.3], "XYStage": [ 21059, 31508 ] }, "GridColumnIndex": 0 }, { "Label": "Pos9", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.82], "Focus": [17741.3], "XYStage": [ 19731.9, 31506.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos10", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.79], "Focus": [17741.4], "XYStage": [ 14528.5, 32007.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos11", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.83], "Focus": [17741.2], "XYStage": [ 13830.300000000001, 32009.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos12", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.79], "Focus": [17741.3], "XYStage": [ 8775.300000000001, 32017.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos13", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.815], "Focus": [17741.3], "XYStage": [ 8109.200000000001, 32008.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos14", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.805], "Focus": [17741.3], "XYStage": [ 3483.3, 31908.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos15", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.82], "Focus": [17741.2], "XYStage": [ 2686.8, 31903.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos16", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [211.48000000000002], "Focus": [17741.1], "XYStage": [ -1978.9, 31973.600000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos17", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [211.51500000000001], "Focus": [17741.1], "XYStage": [ -2957.8, 31982.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos18", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.195], "Focus": [17741.5], "XYStage": [ 47747.4, -576.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos19", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.23000000000002], "Focus": [17741.6], "XYStage": [ 47053.9, -576.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos20", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.205], "Focus": [17741.4], "XYStage": [ 41871.200000000004, -479.8 ] }, "GridColumnIndex": 0 }, { "Label": "Pos21", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.4], "XYStage": [ 40882.600000000006, -392 ] }, "GridColumnIndex": 0 }, { "Label": "Pos22", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.24], "Focus": [17741.4], "XYStage": [ 35905.6, -135.3 ] }, "GridColumnIndex": 0 }, { "Label": "Pos23", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.195], "Focus": [17741.5], "XYStage": [ 34990.9, -132.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos24", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.46], "Focus": [17741.4], "XYStage": [ 29678.100000000002, -958.1 ] }, "GridColumnIndex": 0 }, { "Label": "Pos25", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.44], "Focus": [17741.5], "XYStage": [ 28983.600000000002, -939.4000000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos26", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.475], "Focus": [17741.5], "XYStage": [ 23019.7, -122 ] }, "GridColumnIndex": 0 }, { "Label": "Pos27", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.44], "Focus": [17741.7], "XYStage": [ 22428, -116.80000000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos28", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.46], "Focus": [17741.7], "XYStage": [ 16364.900000000001, -181.10000000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos29", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.48000000000002], "Focus": [17741.5], "XYStage": [ 15712.1, -180 ] }, "GridColumnIndex": 0 }, { "Label": "Pos30", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.45000000000002], "Focus": [17741.7], "XYStage": [ 10346.1, 77.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos31", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.49], "Focus": [17741.4], "XYStage": [ 9755.5, 85.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos32", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.475], "Focus": [17741.5], "XYStage": [ 4775.5, 293.90000000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos33", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.445], "Focus": [17741.4], "XYStage": [ 3954.9, 293.90000000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos34", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.455], "Focus": [17741.4], "XYStage": [ -1324.9, 545.6 ] }, "GridColumnIndex": 0 }, { "Label": "Pos35", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.445], "Focus": [17741.3], "XYStage": [ -2097, 576.6 ] }, "GridColumnIndex": 0 } ], "z-step_um": 10, "GridRow": 0, "BitDepth": 16, "Depth": 2, "Channels": 6, "Source": "Micro-Manager", "ChColors": [ -16711681, -256, -65281, -1, -1, -16776961 ], "MetadataVersion": 10, "KeepShutterOpenChannels": true, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536, 65536 ], "CustomIntervals_ms": [], "Height": 1200, "MicroManagerVersion": "1.4.23 20180219", "GridColumn": 0, "UUID": "18808e7c-f2e6-47b3-90d8-1d779ce87dbd", "PixelType": "GRAY16", "Width": 1200, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\Yan\\20191218-YC113-S7S8-miseq", "Frames": 1, "Comment": "", "UserName": "Nikon", "PixelSize_um": 1.1, "ROI": [ 0, 0, 1200, 1200 ], "Date": "2019-12-19", "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC", "P-DAPI-high" ], "KeepShutterOpenSlices": true, "IJType": 1, "ComputerName": "ZadorConfocal2", "Positions": 36, "PositionIndex": 34, "CameraTimeout": 20000, "TimeFirst": false }, "640 AO-Name": "AnalogIO", "Vincent-D3-Command": "Close" }, "FrameKey-0-3-0": { "COM4-StopBits": "1", "528 AO-MaxVolts": "5.0000", "XLIGHT Excitation Wheel-State": "1", "470 Shutter-Name": "AnalogIO", "Core-Shutter": "640", "555 AO-Demo": "No", "XLIGHT Dichroic-State": "2", "XYStage-StepSize-Y": "0.1000", "ZPositionUm": 168.455, "XYStage-StepSize-X": "0.1000", "PRIME-FirmwareVersion": "20.28", "PRIME-ClearCycles": "2", "520-Name": "DA Shutter", "405 Shutter-Name": "AnalogIO", "PIEZO-StepSize": "0.0050", "PRIME-ClearMode": "Pre-Exposure", "470 AO-MinVolts": "0.0000", "405 AO-SupportsTriggering": "1", "CameraChannelIndex": 0, "XYStage-StartSpeed": "500.0000", "COM9-StopBits": "2", "405 AO-Description": "NI DAC", "COM4-DelayBetweenCharsMs": "0.0000", "445 AO-Block voltage": "false", "520 AO-TriggerInputLine": "/Dev1/PFI0", "555 AO-Description": "NI DAC", "520 AO-TriggerSampleRate": "100000", "COM4-Verbose": "1", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM4-Name": "COM4", "405 Shutter-Volts": "0.0000", "Vincent-D3-Port": "COM1", "PRIME-PP 4 MIN ADU AFFECTED": "200", "470 Shutter-MinVolts": "0.0000", "PRIME-PP 3 ENABLED": "Yes", "470 AO-Description": "NI DAC", "PRIME-Description": "PVCAM API device adapter", "520 Shutter-MaxVolts": "5.0000", "520 Shutter-Volts": "0.0000", "528 AO-Sequenceable": "1", "PRIME-Binning": "1x1", "555 Shutter-MaxVolts": "5.0000", "528 Shutter-TriggerSequenceLength": "1000", "405 Shutter-Block voltage": "false", "XLIGHT Spin motor-State": "1", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "Vincent-D3-Time to open (ms)": "35.0000", "Vincent-D3-Time to close (ms)": "35.0000", "445 AO-TriggerSampleRate": "100000", "640 Shutter-IOChannel": "Dev1/ao14", "555 AO-Sequenceable": "1", "520 AO-Name": "AnalogIO", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "470 AO-Block voltage": "false", "COM1-Verbose": "1", "COM9-AnswerTimeout": "10000.0000", "528 Shutter-Name": "AnalogIO", "PRIME-Trigger Timeout (secs)": "10", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "NextFrame": 0, "470 AO-TriggerSequenceLength": "1000", "PRIME-TransposeXY": "0", "445 AO-TriggerSequenceLength": "1000", "COM1-Name": "COM1", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "COM9-DataBits": "8", "COM1-AnswerTimeout": "500.0000", "Camera": "", "TE2000-Version": "V2.50-V2.50-V2.10", "528 Shutter-Volts": "0.0000", "XLIGHT Hub-Name": "XLIGHT Hub", "PIEZO-Description": "Ludl stage driver adapter", "PositionName": "Pos34", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSampleRate": "100000", "PRIME-FanSpeedSetpoint": "High", "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "405 AO-TriggerSampleRate": "100000", "470 Shutter-Block voltage": "false", "470-State": "0", "COM3-Parity": "None", "405 AO-Volts": "0.0000", "PRIME-TransposeMirrorY": "0", "PRIME-ChipName": "GS144BSI", "470 AO-Volts": "0.0000", "PRIME-TransposeMirrorX": "0", "445 Shutter-SupportsTriggering": "0", "470 Shutter-SupportsTriggering": "0", "XLIGHT Hub-Description": "XLIGHT hub", "PRIME-PreampOffLimit": "10000", "470 AO-Sequenceable": "1", "528 Shutter-MaxVolts": "5.0000", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "Core-XYStage": "XYStage", "470 AO-TriggerSampleRate": "100000", "PRIME-Timing-PreTriggerDelayNs": "24672000.0000", "528-DA Device": "528 Shutter", "Focus-Description": "Nikon TE2000 focus adapter", "FilterBlock-State": "5", "520 AO-Demo": "No", "COM3-DelayBetweenCharsMs": "0.0000", "470 Shutter-Volts": "0.0000", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "PRIME-PP 2 THRESHOLD": "125", "PRIME-PP 4 THRESHOLD": "75", "XYStage-Acceleration": "4.0000", "470 Shutter-Demo": "No", "PRIME-Name": "PMPCIECam00", "640-DA Device": "640 Shutter", "405 AO-Block voltage": "false", "ElapsedTime-ms": 1410237, "PRIME-Offset": "100", "445 AO-IOChannel": "Dev1/ao1", "640 AO-MaxVolts": "5.0000", "PositionIndex": 34, "PRIME-PP 1 ENABLED": "Yes", "COM1-BaudRate": "9600", "640-State": "1", "COM3-Verbose": "1", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "LudlController-TransmissionDelay": "0", "PRIME-FullWellCapacity": "62000", "PRIME-SMARTStreamingEnabled": "No", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "640 Shutter-Description": "NI DAC", "528-Description": "DA device that is used as a shutter", "COM4-Description": "Serial port driver (boost:asio)", "405 Shutter-Description": "NI DAC", "TE2000-Name": "TE2000", "528 Shutter-Description": "NI DAC", "COM9-Description": "Serial port driver (boost:asio)", "LudlController-Name": "LudlController", "445 Shutter-TriggerSequenceLength": "1000", "PRIME-SerialNumber": "A18B203008", "445 AO-Volts": "0.0000", "PRIME-X-dimension": "1200", "PRIME-ShutterCloseDelay": "0", "XLIGHT Disk slider-State": "2", "Position": "Default", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 AO-IOChannel": "Dev1/ao5", "520 AO-IOChannel": "Dev1/ao4", "528 AO-TriggerSequenceLength": "1000", "405 Shutter-MaxVolts": "5.0000", "470-DA Device": "470 Shutter", "PRIME-FTCapable": "No", "Core-ChannelGroup": "Channels", "555 AO-SupportsTriggering": "0", "PRIME-Y-dimension": "1200", "Frame": 0, "555 Shutter-Sequenceable": "1", "PRIME-Timing-ExposureTimeNs": "499998000.0000", "Core-ImageProcessor": "", "528-State": "0", "520 AO-Block voltage": "false", "XLIGHT Excitation Wheel-Label": "QUAD", "640 Shutter-Demo": "No", "COM3-StopBits": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "520 Shutter-IOChannel": "Dev1/ao11", "Width": 1200, "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-SupportsTriggering": "0", "640 AO-TriggerSampleRate": "100000", "520-Description": "DA device that is used as a shutter", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "405 Shutter-Sequenceable": "1", "405 Shutter-SupportsTriggering": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-TriggerSampleRate": "100000", "640 Shutter-MaxVolts": "5.0000", "555 AO-MaxVolts": "5.0000", "520 AO-TriggerSequenceLength": "1000", "COM3-BaudRate": "9600", "COM9-BaudRate": "9600", "Vincent-D3-Channel #": "Ch. #1", "PRIME-PMode": "Normal", "OpticalPath-Label": "Left(100)", "BitDepth": 16, "555 AO-TriggerSequenceLength": "1000", "445 Shutter-Description": "NI DAC", "405 AO-Sequenceable": "1", "PRIME-AcquisitionMethod": "Callbacks", "555 AO-Name": "AnalogIO", "PRIME-ShutterMode": "Pre-Exposure", "COM3-Handshaking": "Off", "445 AO-SupportsTriggering": "0", "ROI": "0-0-1200-1200", "COM9-Verbose": "1", "PRIME-Color": "OFF", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "PRIME-ShutterOpenDelay": "0", "COM1-Parity": "None", "Slice": 0, "SlicePosition": -50, "COM9-DelayBetweenCharsMs": "0.0000", "555-Name": "DA Shutter", "PIEZO-LudlSingleAxisName": "B", "XLIGHT Emission Wheel-Label": "725/40 (C)", "640 Shutter-Name": "AnalogIO", "640 AO-Sequenceable": "1", "520 Shutter-Demo": "No", "528 Shutter-Demo": "No", "470-Name": "DA Shutter", "520-DA Device": "520 Shutter", "XYStage-Description": "Ludl XY stage driver adapter", "445 Shutter-MinVolts": "0.0000", "528 AO-TriggerSampleRate": "100000", "405 AO-MinVolts": "0.0000", "405 Shutter-TriggerSequenceLength": "1000", "Height": 1200, "555 AO-Block voltage": "false", "640 Shutter-Volts": "5.0000", "PixelType": "GRAY16", "445 Shutter-Block voltage": "false", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "520 Shutter-TriggerSequenceLength": "1000", "COM9-Name": "COM9", "Core-SLM": "", "555 AO-Volts": "0.0000", "555 Shutter-Block voltage": "false", "XYStage-Speed": "1800.0000", "520 AO-MaxVolts": "5.0000", "528 Shutter-SupportsTriggering": "0", "COM1-Description": "Serial port driver (boost:asio)", "PRIME-MetadataEnabled": "No", "520 Shutter-MinVolts": "0.0000", "470 Shutter-Sequenceable": "1", "PRIME-PreampDelay": "0", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-MinVolts": "0.0000", "ChannelIndex": 3, "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Core-AutoShutter": "1", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "Exposure-ms": 520, "PRIME-PP 2 ENABLED": "Yes", "640 Shutter-TriggerSampleRate": "100000", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "470 Shutter-IOChannel": "Dev1/ao10", "405 AO-Demo": "No", "PixelSizeUm": 1.1, "470 AO-Demo": "No", "470 Shutter-MaxVolts": "5.0000", "Vincent-D3-Name": "Vincent-D3", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FileName": "img_000000000_Il-C_000.tif", "LudlController-Reset": "Operate", "PRIME-Exposure": "520.00", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "445-Name": "DA Shutter", "555 Shutter-Name": "AnalogIO", "520 Shutter-TriggerSampleRate": "100000", "445 Shutter-IOChannel": "Dev1/ao9", "PRIME-TransposeCorrection": "0", "XYStage-StepSize": "0.1000", "445-Description": "DA device that is used as a shutter", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 AO-IOChannel": "Dev1/ao7", "520 Shutter-Name": "AnalogIO", "COM3-DataBits": "8", "640 AO-Volts": "4.5000", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "555 Shutter-IOChannel": "Dev1/ao13", "WaitInterval": null, "520 Shutter-Sequenceable": "1", "YPositionUm": 545.6, "445-State": "0", "445-DA Device": "445 Shutter", "PRIME-CircularBufferAutoSize": "ON", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "555 AO-TriggerSampleRate": "100000", "445 Shutter-Volts": "0.0000", "XLIGHT Hub-Port": "COM3", "Core-AutoFocus": "", "COM4-Handshaking": "Off", "528 Shutter-MinVolts": "0.0000", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "445 Shutter-Name": "AnalogIO", "528 Shutter-Sequenceable": "1", "XYStage-TransposeMirrorX": "0", "XYStage-TransposeMirrorY": "0", "PRIME-ReadoutRate": "100MHz 16bit", "XYStage-Name": "Stage", "640 AO-SupportsTriggering": "0", "640-Name": "DA Shutter", "640 Shutter-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "640 AO-Block voltage": "false", "445 Shutter-Sequenceable": "1", "470 AO-MaxVolts": "5.0000", "COM4-BaudRate": "9600", "470-Description": "DA device that is used as a shutter", "520 Shutter-Description": "NI DAC", "PRIME-CCDTemperature": "-14.8500", "Time": "2019-12-19 09:59:40 -0500", "640 AO-TriggerSequenceLength": "1000", "470 AO-Name": "AnalogIO", "XLIGHT Disk slider-Label": "Disk Pos 40um", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "Binning": "1x1", "TE2000-ModelType": "1", "COM3-AnswerTimeout": "500.0000", "555 Shutter-SupportsTriggering": "0", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "TE2000-Port": "COM4", "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Description": "NI DAC", "COM4-AnswerTimeout": "500.0000", "COM1-DelayBetweenCharsMs": "0.0000", "FilterBlock-Label": "Confocal", "445 AO-Name": "AnalogIO", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-Timing-ReadoutTimeNs": "24672000.0000", "528 AO-Demo": "No", "520 AO-Sequenceable": "1", "555-Description": "DA device that is used as a shutter", "405-DA Device": "405 Shutter", "405 AO-TriggerSequenceLength": "1000", "640 AO-MinVolts": "0.0000", "640 Shutter-Block voltage": "false", "FrameIndex": 0, "PRIME-Port": "CMOS", "555 AO-MinVolts": "0.0000", "COM3-Description": "Serial port driver (boost:asio)", "528 AO-Description": "NI DAC", "555 AO-IOChannel": "Dev1/ao6", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "555 AO-TriggerInputLine": "/Dev1/PFI0", "PRIME-ActualInterval-ms": "390.5200", "LudlController-Port": "COM9", "445 Shutter-Demo": "No", "PRIME-Trigger-Expose Out-Mux": "1", "SliceIndex": 0, "PRIME-CCDTemperatureSetPoint": "-15.0000", "405 Shutter-MinVolts": "0.0000", "PRIME-ExposeOutMode": "First Row", "AxisPositions": null, "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Volts": "0.0000", "528-Name": "DA Shutter", "555-State": "0", "XLIGHT Dichroic-Label": "Di03-R635-t1", "445 AO-Sequenceable": "1", "405-Name": "DA Shutter", "405 AO-IOChannel": "Dev1/ao0", "Core-Camera": "PRIME", "Focus-Name": "Focus", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "405 Shutter-IOChannel": "Dev1/ao8", "640 AO-Demo": "No", "640 Shutter-SupportsTriggering": "0", "445 AO-Demo": "No", "555-DA Device": "555 Shutter", "470 Shutter-Description": "NI DAC", "528 AO-Volts": "0.0000", "COM1-DataBits": "8", "Vincent-D3-Address": "x", "520 AO-SupportsTriggering": "0", "405-Description": "DA device that is used as a shutter", "640 Shutter-MinVolts": "0.0000", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "405 AO-Name": "AnalogIO", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "640 AO-TriggerInputLine": "/Dev1/PFI0", "405 AO-TriggerInputLine": "/Dev1/PFI0", "640-Description": "DA device that is used as a shutter", "445 Shutter-TriggerSampleRate": "100000", "470 Shutter-TriggerSequenceLength": "1000", "COM1-StopBits": "1", "520 Shutter-SupportsTriggering": "0", "405-State": "0", "Core-Initialize": "1", "XLIGHT Emission Wheel-State": "3", "520 AO-Description": "NI DAC", "528 AO-TriggerInputLine": "/Dev1/PFI0", "445 Shutter-MaxVolts": "5.0000", "COM9-Handshaking": "Off", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "XLIGHT Spin motor-Label": "On (spinning)", "PRIME-PP 5 ENABLED": "No", "UUID": "775e3376-b24d-4293-b114-4ab93dbee2fb", "PRIME-TriggerMode": "Internal Trigger", "COM1-Handshaking": "Off", "PRIME-Timing-PostTriggerDelayNs": "49344000.0000", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "Focus-Position": "17741.5000", "528 Shutter-Block voltage": "false", "Channel": "Il-C", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "555 Shutter-Demo": "No", "405 Shutter-Demo": "No", "528 AO-Name": "AnalogIO", "520-State": "0", "Core-Galvo": "", "405 Shutter-TriggerSampleRate": "100000", "Focus-FocusStepSize_(nm)": "100", "PRIME-PixelType": "16bit", "COM4-DataBits": "8", "470 AO-IOChannel": "Dev1/ao2", "520 AO-MinVolts": "0.0000", "COM9-Parity": "None", "PRIME-CircularBufferEnabled": "ON", "PRIME-PP 6 Reset": "No", "640 AO-Description": "NI DAC", "Source": "PRIME", "445 AO-MinVolts": "0.0000", "PIEZO-Name": "Stage", "445 AO-Description": "NI DAC", "PRIME-CircularBufferFrameCount": "46", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "520 AO-Volts": "0.0000", "528 Shutter-TriggerSampleRate": "100000", "XPositionUm": -1324.9, "OpticalPath-State": "4", "PRIME-PP 3 THRESHOLD": "80", "PRIME-PVCAM Version": "3.7.1", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Gain": "1-HDR", "640 Shutter-TriggerSequenceLength": "1000", "528 AO-SupportsTriggering": "0", "445 AO-TriggerInputLine": "/Dev1/PFI0", "OpticalPath-Name": "OpticalPath", "PRIME-PP 4 ENABLED": "Yes", "PRIME-PVCAM Adapter Version": "1.3.29", "COM3-Name": "COM3", "405 AO-MaxVolts": "5.0000", "520 Shutter-Block voltage": "false", "PRIME-Timing-ClearingTimeNs": "49344000.0000", "Core-Focus": "PIEZO", "528 AO-Block voltage": "false", "TE2000-Description": "Nikon TE2000 hub", "528 AO-MinVolts": "0.0000", "COM4-Parity": "None", "Summary": { "Slices": 11, "ChContrastMin": [ 0, 0, 0, 0, 0, 0 ], "PixelAspect": 1, "SlicesFirst": true, "Interval_ms": 0, "Time": "2019-12-19 09:59:21 -0500", "Prefix": "seq_13", "InitialPositionList": [ { "Label": "Pos0", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.21], "Focus": [17741.5], "XYStage": [ 44542.200000000004, 31636.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos1", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.22], "Focus": [17741.4], "XYStage": [ 43801.100000000006, 31637.9 ] }, "GridColumnIndex": 0 }, { "Label": "Pos2", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.5], "XYStage": [ 38561.3, 32040.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos3", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.185], "Focus": [17741.3], "XYStage": [ 37698.4, 32041.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos4", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.16], "Focus": [17741.5], "XYStage": [ 32375.4, 31758.9 ] }, "GridColumnIndex": 0 }, { "Label": "Pos5", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.17000000000002], "Focus": [17741.3], "XYStage": [ 31770.7, 31758.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos6", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.4], "XYStage": [ 26536.800000000003, 31068.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos7", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.205], "Focus": [17741.4], "XYStage": [ 25420.2, 31068.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos8", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.85], "Focus": [17741.3], "XYStage": [ 21059, 31508 ] }, "GridColumnIndex": 0 }, { "Label": "Pos9", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.82], "Focus": [17741.3], "XYStage": [ 19731.9, 31506.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos10", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.79], "Focus": [17741.4], "XYStage": [ 14528.5, 32007.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos11", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.83], "Focus": [17741.2], "XYStage": [ 13830.300000000001, 32009.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos12", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.79], "Focus": [17741.3], "XYStage": [ 8775.300000000001, 32017.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos13", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.815], "Focus": [17741.3], "XYStage": [ 8109.200000000001, 32008.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos14", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.805], "Focus": [17741.3], "XYStage": [ 3483.3, 31908.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos15", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.82], "Focus": [17741.2], "XYStage": [ 2686.8, 31903.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos16", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [211.48000000000002], "Focus": [17741.1], "XYStage": [ -1978.9, 31973.600000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos17", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [211.51500000000001], "Focus": [17741.1], "XYStage": [ -2957.8, 31982.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos18", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.195], "Focus": [17741.5], "XYStage": [ 47747.4, -576.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos19", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.23000000000002], "Focus": [17741.6], "XYStage": [ 47053.9, -576.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos20", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.205], "Focus": [17741.4], "XYStage": [ 41871.200000000004, -479.8 ] }, "GridColumnIndex": 0 }, { "Label": "Pos21", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.4], "XYStage": [ 40882.600000000006, -392 ] }, "GridColumnIndex": 0 }, { "Label": "Pos22", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.24], "Focus": [17741.4], "XYStage": [ 35905.6, -135.3 ] }, "GridColumnIndex": 0 }, { "Label": "Pos23", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.195], "Focus": [17741.5], "XYStage": [ 34990.9, -132.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos24", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.46], "Focus": [17741.4], "XYStage": [ 29678.100000000002, -958.1 ] }, "GridColumnIndex": 0 }, { "Label": "Pos25", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.44], "Focus": [17741.5], "XYStage": [ 28983.600000000002, -939.4000000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos26", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.475], "Focus": [17741.5], "XYStage": [ 23019.7, -122 ] }, "GridColumnIndex": 0 }, { "Label": "Pos27", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.44], "Focus": [17741.7], "XYStage": [ 22428, -116.80000000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos28", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.46], "Focus": [17741.7], "XYStage": [ 16364.900000000001, -181.10000000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos29", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.48000000000002], "Focus": [17741.5], "XYStage": [ 15712.1, -180 ] }, "GridColumnIndex": 0 }, { "Label": "Pos30", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.45000000000002], "Focus": [17741.7], "XYStage": [ 10346.1, 77.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos31", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.49], "Focus": [17741.4], "XYStage": [ 9755.5, 85.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos32", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.475], "Focus": [17741.5], "XYStage": [ 4775.5, 293.90000000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos33", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.445], "Focus": [17741.4], "XYStage": [ 3954.9, 293.90000000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos34", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.455], "Focus": [17741.4], "XYStage": [ -1324.9, 545.6 ] }, "GridColumnIndex": 0 }, { "Label": "Pos35", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.445], "Focus": [17741.3], "XYStage": [ -2097, 576.6 ] }, "GridColumnIndex": 0 } ], "z-step_um": 10, "GridRow": 0, "BitDepth": 16, "Depth": 2, "Channels": 6, "Source": "Micro-Manager", "ChColors": [ -16711681, -256, -65281, -1, -1, -16776961 ], "MetadataVersion": 10, "KeepShutterOpenChannels": true, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536, 65536 ], "CustomIntervals_ms": [], "Height": 1200, "MicroManagerVersion": "1.4.23 20180219", "GridColumn": 0, "UUID": "18808e7c-f2e6-47b3-90d8-1d779ce87dbd", "PixelType": "GRAY16", "Width": 1200, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\Yan\\20191218-YC113-S7S8-miseq", "Frames": 1, "Comment": "", "UserName": "Nikon", "PixelSize_um": 1.1, "ROI": [ 0, 0, 1200, 1200 ], "Date": "2019-12-19", "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC", "P-DAPI-high" ], "KeepShutterOpenSlices": true, "IJType": 1, "ComputerName": "ZadorConfocal2", "Positions": 36, "PositionIndex": 34, "CameraTimeout": 20000, "TimeFirst": false }, "640 AO-Name": "AnalogIO", "Vincent-D3-Command": "Close" }, "FrameKey-0-3-1": { "COM4-StopBits": "1", "528 AO-MaxVolts": "5.0000", "XLIGHT Excitation Wheel-State": "1", "470 Shutter-Name": "AnalogIO", "Core-Shutter": "640", "555 AO-Demo": "No", "XLIGHT Dichroic-State": "2", "XYStage-StepSize-Y": "0.1000", "ZPositionUm": 178.455, "XYStage-StepSize-X": "0.1000", "PRIME-FirmwareVersion": "20.28", "PRIME-ClearCycles": "2", "520-Name": "DA Shutter", "405 Shutter-Name": "AnalogIO", "PIEZO-StepSize": "0.0050", "PRIME-ClearMode": "Pre-Exposure", "470 AO-MinVolts": "0.0000", "405 AO-SupportsTriggering": "1", "CameraChannelIndex": 0, "XYStage-StartSpeed": "500.0000", "COM9-StopBits": "2", "405 AO-Description": "NI DAC", "COM4-DelayBetweenCharsMs": "0.0000", "445 AO-Block voltage": "false", "520 AO-TriggerInputLine": "/Dev1/PFI0", "555 AO-Description": "NI DAC", "520 AO-TriggerSampleRate": "100000", "COM4-Verbose": "1", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM4-Name": "COM4", "405 Shutter-Volts": "0.0000", "Vincent-D3-Port": "COM1", "PRIME-PP 4 MIN ADU AFFECTED": "200", "470 Shutter-MinVolts": "0.0000", "PRIME-PP 3 ENABLED": "Yes", "470 AO-Description": "NI DAC", "PRIME-Description": "PVCAM API device adapter", "520 Shutter-MaxVolts": "5.0000", "520 Shutter-Volts": "0.0000", "528 AO-Sequenceable": "1", "PRIME-Binning": "1x1", "555 Shutter-MaxVolts": "5.0000", "528 Shutter-TriggerSequenceLength": "1000", "405 Shutter-Block voltage": "false", "XLIGHT Spin motor-State": "1", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "Vincent-D3-Time to open (ms)": "35.0000", "Vincent-D3-Time to close (ms)": "35.0000", "445 AO-TriggerSampleRate": "100000", "640 Shutter-IOChannel": "Dev1/ao14", "555 AO-Sequenceable": "1", "520 AO-Name": "AnalogIO", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "470 AO-Block voltage": "false", "COM1-Verbose": "1", "COM9-AnswerTimeout": "10000.0000", "528 Shutter-Name": "AnalogIO", "PRIME-Trigger Timeout (secs)": "10", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "NextFrame": 0, "470 AO-TriggerSequenceLength": "1000", "PRIME-TransposeXY": "0", "445 AO-TriggerSequenceLength": "1000", "COM1-Name": "COM1", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "COM9-DataBits": "8", "COM1-AnswerTimeout": "500.0000", "Camera": "", "TE2000-Version": "V2.50-V2.50-V2.10", "528 Shutter-Volts": "0.0000", "XLIGHT Hub-Name": "XLIGHT Hub", "PIEZO-Description": "Ludl stage driver adapter", "PositionName": "Pos34", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSampleRate": "100000", "PRIME-FanSpeedSetpoint": "High", "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "405 AO-TriggerSampleRate": "100000", "470 Shutter-Block voltage": "false", "470-State": "0", "COM3-Parity": "None", "405 AO-Volts": "0.0000", "PRIME-TransposeMirrorY": "0", "PRIME-ChipName": "GS144BSI", "470 AO-Volts": "0.0000", "PRIME-TransposeMirrorX": "0", "445 Shutter-SupportsTriggering": "0", "470 Shutter-SupportsTriggering": "0", "XLIGHT Hub-Description": "XLIGHT hub", "PRIME-PreampOffLimit": "10000", "470 AO-Sequenceable": "1", "528 Shutter-MaxVolts": "5.0000", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "Core-XYStage": "XYStage", "470 AO-TriggerSampleRate": "100000", "PRIME-Timing-PreTriggerDelayNs": "24672000.0000", "528-DA Device": "528 Shutter", "Focus-Description": "Nikon TE2000 focus adapter", "FilterBlock-State": "5", "520 AO-Demo": "No", "COM3-DelayBetweenCharsMs": "0.0000", "470 Shutter-Volts": "0.0000", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "PRIME-PP 2 THRESHOLD": "125", "PRIME-PP 4 THRESHOLD": "75", "XYStage-Acceleration": "4.0000", "470 Shutter-Demo": "No", "PRIME-Name": "PMPCIECam00", "640-DA Device": "640 Shutter", "405 AO-Block voltage": "false", "ElapsedTime-ms": 1411048, "PRIME-Offset": "100", "445 AO-IOChannel": "Dev1/ao1", "640 AO-MaxVolts": "5.0000", "PositionIndex": 34, "PRIME-PP 1 ENABLED": "Yes", "COM1-BaudRate": "9600", "640-State": "1", "COM3-Verbose": "1", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "LudlController-TransmissionDelay": "0", "PRIME-FullWellCapacity": "62000", "PRIME-SMARTStreamingEnabled": "No", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "640 Shutter-Description": "NI DAC", "528-Description": "DA device that is used as a shutter", "COM4-Description": "Serial port driver (boost:asio)", "405 Shutter-Description": "NI DAC", "TE2000-Name": "TE2000", "528 Shutter-Description": "NI DAC", "COM9-Description": "Serial port driver (boost:asio)", "LudlController-Name": "LudlController", "445 Shutter-TriggerSequenceLength": "1000", "PRIME-SerialNumber": "A18B203008", "445 AO-Volts": "0.0000", "PRIME-X-dimension": "1200", "PRIME-ShutterCloseDelay": "0", "XLIGHT Disk slider-State": "2", "Position": "Default", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 AO-IOChannel": "Dev1/ao5", "520 AO-IOChannel": "Dev1/ao4", "528 AO-TriggerSequenceLength": "1000", "405 Shutter-MaxVolts": "5.0000", "470-DA Device": "470 Shutter", "PRIME-FTCapable": "No", "Core-ChannelGroup": "Channels", "555 AO-SupportsTriggering": "0", "PRIME-Y-dimension": "1200", "Frame": 0, "555 Shutter-Sequenceable": "1", "PRIME-Timing-ExposureTimeNs": "499998000.0000", "Core-ImageProcessor": "", "528-State": "0", "520 AO-Block voltage": "false", "XLIGHT Excitation Wheel-Label": "QUAD", "640 Shutter-Demo": "No", "COM3-StopBits": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "520 Shutter-IOChannel": "Dev1/ao11", "Width": 1200, "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-SupportsTriggering": "0", "640 AO-TriggerSampleRate": "100000", "520-Description": "DA device that is used as a shutter", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "405 Shutter-Sequenceable": "1", "405 Shutter-SupportsTriggering": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-TriggerSampleRate": "100000", "640 Shutter-MaxVolts": "5.0000", "555 AO-MaxVolts": "5.0000", "520 AO-TriggerSequenceLength": "1000", "COM3-BaudRate": "9600", "COM9-BaudRate": "9600", "Vincent-D3-Channel #": "Ch. #1", "PRIME-PMode": "Normal", "OpticalPath-Label": "Left(100)", "BitDepth": 16, "555 AO-TriggerSequenceLength": "1000", "445 Shutter-Description": "NI DAC", "405 AO-Sequenceable": "1", "PRIME-AcquisitionMethod": "Callbacks", "555 AO-Name": "AnalogIO", "PRIME-ShutterMode": "Pre-Exposure", "COM3-Handshaking": "Off", "445 AO-SupportsTriggering": "0", "ROI": "0-0-1200-1200", "COM9-Verbose": "1", "PRIME-Color": "OFF", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "PRIME-ShutterOpenDelay": "0", "COM1-Parity": "None", "Slice": 1, "SlicePosition": -40, "COM9-DelayBetweenCharsMs": "0.0000", "555-Name": "DA Shutter", "PIEZO-LudlSingleAxisName": "B", "XLIGHT Emission Wheel-Label": "725/40 (C)", "640 Shutter-Name": "AnalogIO", "640 AO-Sequenceable": "1", "520 Shutter-Demo": "No", "528 Shutter-Demo": "No", "470-Name": "DA Shutter", "520-DA Device": "520 Shutter", "XYStage-Description": "Ludl XY stage driver adapter", "445 Shutter-MinVolts": "0.0000", "528 AO-TriggerSampleRate": "100000", "405 AO-MinVolts": "0.0000", "405 Shutter-TriggerSequenceLength": "1000", "Height": 1200, "555 AO-Block voltage": "false", "640 Shutter-Volts": "5.0000", "PixelType": "GRAY16", "445 Shutter-Block voltage": "false", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "520 Shutter-TriggerSequenceLength": "1000", "COM9-Name": "COM9", "Core-SLM": "", "555 AO-Volts": "0.0000", "555 Shutter-Block voltage": "false", "XYStage-Speed": "1800.0000", "520 AO-MaxVolts": "5.0000", "528 Shutter-SupportsTriggering": "0", "COM1-Description": "Serial port driver (boost:asio)", "PRIME-MetadataEnabled": "No", "520 Shutter-MinVolts": "0.0000", "470 Shutter-Sequenceable": "1", "PRIME-PreampDelay": "0", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-MinVolts": "0.0000", "ChannelIndex": 3, "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Core-AutoShutter": "1", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "Exposure-ms": 520, "PRIME-PP 2 ENABLED": "Yes", "640 Shutter-TriggerSampleRate": "100000", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "470 Shutter-IOChannel": "Dev1/ao10", "405 AO-Demo": "No", "PixelSizeUm": 1.1, "470 AO-Demo": "No", "470 Shutter-MaxVolts": "5.0000", "Vincent-D3-Name": "Vincent-D3", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FileName": "img_000000000_Il-C_001.tif", "LudlController-Reset": "Operate", "PRIME-Exposure": "520.00", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "445-Name": "DA Shutter", "555 Shutter-Name": "AnalogIO", "520 Shutter-TriggerSampleRate": "100000", "445 Shutter-IOChannel": "Dev1/ao9", "PRIME-TransposeCorrection": "0", "XYStage-StepSize": "0.1000", "445-Description": "DA device that is used as a shutter", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 AO-IOChannel": "Dev1/ao7", "520 Shutter-Name": "AnalogIO", "COM3-DataBits": "8", "640 AO-Volts": "4.5000", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "555 Shutter-IOChannel": "Dev1/ao13", "WaitInterval": null, "520 Shutter-Sequenceable": "1", "YPositionUm": 545.6, "445-State": "0", "445-DA Device": "445 Shutter", "PRIME-CircularBufferAutoSize": "ON", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "555 AO-TriggerSampleRate": "100000", "445 Shutter-Volts": "0.0000", "XLIGHT Hub-Port": "COM3", "Core-AutoFocus": "", "COM4-Handshaking": "Off", "528 Shutter-MinVolts": "0.0000", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "445 Shutter-Name": "AnalogIO", "528 Shutter-Sequenceable": "1", "XYStage-TransposeMirrorX": "0", "XYStage-TransposeMirrorY": "0", "PRIME-ReadoutRate": "100MHz 16bit", "XYStage-Name": "Stage", "640 AO-SupportsTriggering": "0", "640-Name": "DA Shutter", "640 Shutter-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "640 AO-Block voltage": "false", "445 Shutter-Sequenceable": "1", "470 AO-MaxVolts": "5.0000", "COM4-BaudRate": "9600", "470-Description": "DA device that is used as a shutter", "520 Shutter-Description": "NI DAC", "PRIME-CCDTemperature": "-14.8500", "Time": "2019-12-19 09:59:41 -0500", "640 AO-TriggerSequenceLength": "1000", "470 AO-Name": "AnalogIO", "XLIGHT Disk slider-Label": "Disk Pos 40um", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "Binning": "1x1", "TE2000-ModelType": "1", "COM3-AnswerTimeout": "500.0000", "555 Shutter-SupportsTriggering": "0", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "TE2000-Port": "COM4", "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Description": "NI DAC", "COM4-AnswerTimeout": "500.0000", "COM1-DelayBetweenCharsMs": "0.0000", "FilterBlock-Label": "Confocal", "445 AO-Name": "AnalogIO", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-Timing-ReadoutTimeNs": "24672000.0000", "528 AO-Demo": "No", "520 AO-Sequenceable": "1", "555-Description": "DA device that is used as a shutter", "405-DA Device": "405 Shutter", "405 AO-TriggerSequenceLength": "1000", "640 AO-MinVolts": "0.0000", "640 Shutter-Block voltage": "false", "FrameIndex": 0, "PRIME-Port": "CMOS", "555 AO-MinVolts": "0.0000", "COM3-Description": "Serial port driver (boost:asio)", "528 AO-Description": "NI DAC", "555 AO-IOChannel": "Dev1/ao6", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "555 AO-TriggerInputLine": "/Dev1/PFI0", "PRIME-ActualInterval-ms": "390.5200", "LudlController-Port": "COM9", "445 Shutter-Demo": "No", "PRIME-Trigger-Expose Out-Mux": "1", "SliceIndex": 1, "PRIME-CCDTemperatureSetPoint": "-15.0000", "405 Shutter-MinVolts": "0.0000", "PRIME-ExposeOutMode": "First Row", "AxisPositions": null, "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Volts": "0.0000", "528-Name": "DA Shutter", "555-State": "0", "XLIGHT Dichroic-Label": "Di03-R635-t1", "445 AO-Sequenceable": "1", "405-Name": "DA Shutter", "405 AO-IOChannel": "Dev1/ao0", "Core-Camera": "PRIME", "Focus-Name": "Focus", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "405 Shutter-IOChannel": "Dev1/ao8", "640 AO-Demo": "No", "640 Shutter-SupportsTriggering": "0", "445 AO-Demo": "No", "555-DA Device": "555 Shutter", "470 Shutter-Description": "NI DAC", "528 AO-Volts": "0.0000", "COM1-DataBits": "8", "Vincent-D3-Address": "x", "520 AO-SupportsTriggering": "0", "405-Description": "DA device that is used as a shutter", "640 Shutter-MinVolts": "0.0000", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "405 AO-Name": "AnalogIO", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "640 AO-TriggerInputLine": "/Dev1/PFI0", "405 AO-TriggerInputLine": "/Dev1/PFI0", "640-Description": "DA device that is used as a shutter", "445 Shutter-TriggerSampleRate": "100000", "470 Shutter-TriggerSequenceLength": "1000", "COM1-StopBits": "1", "520 Shutter-SupportsTriggering": "0", "405-State": "0", "Core-Initialize": "1", "XLIGHT Emission Wheel-State": "3", "520 AO-Description": "NI DAC", "528 AO-TriggerInputLine": "/Dev1/PFI0", "445 Shutter-MaxVolts": "5.0000", "COM9-Handshaking": "Off", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "XLIGHT Spin motor-Label": "On (spinning)", "PRIME-PP 5 ENABLED": "No", "UUID": "1fbc96f4-ff45-4cc1-aa81-5dc6123d7b80", "PRIME-TriggerMode": "Internal Trigger", "COM1-Handshaking": "Off", "PRIME-Timing-PostTriggerDelayNs": "49344000.0000", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "Focus-Position": "17741.5000", "528 Shutter-Block voltage": "false", "Channel": "Il-C", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "555 Shutter-Demo": "No", "405 Shutter-Demo": "No", "528 AO-Name": "AnalogIO", "520-State": "0", "Core-Galvo": "", "405 Shutter-TriggerSampleRate": "100000", "Focus-FocusStepSize_(nm)": "100", "PRIME-PixelType": "16bit", "COM4-DataBits": "8", "470 AO-IOChannel": "Dev1/ao2", "520 AO-MinVolts": "0.0000", "COM9-Parity": "None", "PRIME-CircularBufferEnabled": "ON", "PRIME-PP 6 Reset": "No", "640 AO-Description": "NI DAC", "Source": "PRIME", "445 AO-MinVolts": "0.0000", "PIEZO-Name": "Stage", "445 AO-Description": "NI DAC", "PRIME-CircularBufferFrameCount": "46", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "520 AO-Volts": "0.0000", "528 Shutter-TriggerSampleRate": "100000", "XPositionUm": -1324.9, "OpticalPath-State": "4", "PRIME-PP 3 THRESHOLD": "80", "PRIME-PVCAM Version": "3.7.1", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Gain": "1-HDR", "640 Shutter-TriggerSequenceLength": "1000", "528 AO-SupportsTriggering": "0", "445 AO-TriggerInputLine": "/Dev1/PFI0", "OpticalPath-Name": "OpticalPath", "PRIME-PP 4 ENABLED": "Yes", "PRIME-PVCAM Adapter Version": "1.3.29", "COM3-Name": "COM3", "405 AO-MaxVolts": "5.0000", "520 Shutter-Block voltage": "false", "PRIME-Timing-ClearingTimeNs": "49344000.0000", "Core-Focus": "PIEZO", "528 AO-Block voltage": "false", "TE2000-Description": "Nikon TE2000 hub", "528 AO-MinVolts": "0.0000", "COM4-Parity": "None", "Summary": { "Slices": 11, "ChContrastMin": [ 0, 0, 0, 0, 0, 0 ], "PixelAspect": 1, "SlicesFirst": true, "Interval_ms": 0, "Time": "2019-12-19 09:59:21 -0500", "Prefix": "seq_13", "InitialPositionList": [ { "Label": "Pos0", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.21], "Focus": [17741.5], "XYStage": [ 44542.200000000004, 31636.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos1", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.22], "Focus": [17741.4], "XYStage": [ 43801.100000000006, 31637.9 ] }, "GridColumnIndex": 0 }, { "Label": "Pos2", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.5], "XYStage": [ 38561.3, 32040.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos3", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.185], "Focus": [17741.3], "XYStage": [ 37698.4, 32041.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos4", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.16], "Focus": [17741.5], "XYStage": [ 32375.4, 31758.9 ] }, "GridColumnIndex": 0 }, { "Label": "Pos5", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.17000000000002], "Focus": [17741.3], "XYStage": [ 31770.7, 31758.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos6", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.4], "XYStage": [ 26536.800000000003, 31068.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos7", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.205], "Focus": [17741.4], "XYStage": [ 25420.2, 31068.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos8", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.85], "Focus": [17741.3], "XYStage": [ 21059, 31508 ] }, "GridColumnIndex": 0 }, { "Label": "Pos9", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.82], "Focus": [17741.3], "XYStage": [ 19731.9, 31506.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos10", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.79], "Focus": [17741.4], "XYStage": [ 14528.5, 32007.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos11", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.83], "Focus": [17741.2], "XYStage": [ 13830.300000000001, 32009.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos12", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.79], "Focus": [17741.3], "XYStage": [ 8775.300000000001, 32017.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos13", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.815], "Focus": [17741.3], "XYStage": [ 8109.200000000001, 32008.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos14", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.805], "Focus": [17741.3], "XYStage": [ 3483.3, 31908.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos15", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.82], "Focus": [17741.2], "XYStage": [ 2686.8, 31903.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos16", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [211.48000000000002], "Focus": [17741.1], "XYStage": [ -1978.9, 31973.600000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos17", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [211.51500000000001], "Focus": [17741.1], "XYStage": [ -2957.8, 31982.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos18", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.195], "Focus": [17741.5], "XYStage": [ 47747.4, -576.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos19", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.23000000000002], "Focus": [17741.6], "XYStage": [ 47053.9, -576.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos20", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.205], "Focus": [17741.4], "XYStage": [ 41871.200000000004, -479.8 ] }, "GridColumnIndex": 0 }, { "Label": "Pos21", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.4], "XYStage": [ 40882.600000000006, -392 ] }, "GridColumnIndex": 0 }, { "Label": "Pos22", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.24], "Focus": [17741.4], "XYStage": [ 35905.6, -135.3 ] }, "GridColumnIndex": 0 }, { "Label": "Pos23", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.195], "Focus": [17741.5], "XYStage": [ 34990.9, -132.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos24", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.46], "Focus": [17741.4], "XYStage": [ 29678.100000000002, -958.1 ] }, "GridColumnIndex": 0 }, { "Label": "Pos25", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.44], "Focus": [17741.5], "XYStage": [ 28983.600000000002, -939.4000000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos26", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.475], "Focus": [17741.5], "XYStage": [ 23019.7, -122 ] }, "GridColumnIndex": 0 }, { "Label": "Pos27", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.44], "Focus": [17741.7], "XYStage": [ 22428, -116.80000000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos28", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.46], "Focus": [17741.7], "XYStage": [ 16364.900000000001, -181.10000000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos29", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.48000000000002], "Focus": [17741.5], "XYStage": [ 15712.1, -180 ] }, "GridColumnIndex": 0 }, { "Label": "Pos30", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.45000000000002], "Focus": [17741.7], "XYStage": [ 10346.1, 77.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos31", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.49], "Focus": [17741.4], "XYStage": [ 9755.5, 85.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos32", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.475], "Focus": [17741.5], "XYStage": [ 4775.5, 293.90000000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos33", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.445], "Focus": [17741.4], "XYStage": [ 3954.9, 293.90000000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos34", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.455], "Focus": [17741.4], "XYStage": [ -1324.9, 545.6 ] }, "GridColumnIndex": 0 }, { "Label": "Pos35", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.445], "Focus": [17741.3], "XYStage": [ -2097, 576.6 ] }, "GridColumnIndex": 0 } ], "z-step_um": 10, "GridRow": 0, "BitDepth": 16, "Depth": 2, "Channels": 6, "Source": "Micro-Manager", "ChColors": [ -16711681, -256, -65281, -1, -1, -16776961 ], "MetadataVersion": 10, "KeepShutterOpenChannels": true, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536, 65536 ], "CustomIntervals_ms": [], "Height": 1200, "MicroManagerVersion": "1.4.23 20180219", "GridColumn": 0, "UUID": "18808e7c-f2e6-47b3-90d8-1d779ce87dbd", "PixelType": "GRAY16", "Width": 1200, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\Yan\\20191218-YC113-S7S8-miseq", "Frames": 1, "Comment": "", "UserName": "Nikon", "PixelSize_um": 1.1, "ROI": [ 0, 0, 1200, 1200 ], "Date": "2019-12-19", "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC", "P-DAPI-high" ], "KeepShutterOpenSlices": true, "IJType": 1, "ComputerName": "ZadorConfocal2", "Positions": 36, "PositionIndex": 34, "CameraTimeout": 20000, "TimeFirst": false }, "640 AO-Name": "AnalogIO", "Vincent-D3-Command": "Close" }, "FrameKey-0-3-2": { "COM4-StopBits": "1", "528 AO-MaxVolts": "5.0000", "XLIGHT Excitation Wheel-State": "1", "470 Shutter-Name": "AnalogIO", "Core-Shutter": "640", "555 AO-Demo": "No", "XLIGHT Dichroic-State": "2", "XYStage-StepSize-Y": "0.1000", "ZPositionUm": 188.455, "XYStage-StepSize-X": "0.1000", "PRIME-FirmwareVersion": "20.28", "PRIME-ClearCycles": "2", "520-Name": "DA Shutter", "405 Shutter-Name": "AnalogIO", "PIEZO-StepSize": "0.0050", "PRIME-ClearMode": "Pre-Exposure", "470 AO-MinVolts": "0.0000", "405 AO-SupportsTriggering": "1", "CameraChannelIndex": 0, "XYStage-StartSpeed": "500.0000", "COM9-StopBits": "2", "405 AO-Description": "NI DAC", "COM4-DelayBetweenCharsMs": "0.0000", "445 AO-Block voltage": "false", "520 AO-TriggerInputLine": "/Dev1/PFI0", "555 AO-Description": "NI DAC", "520 AO-TriggerSampleRate": "100000", "COM4-Verbose": "1", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM4-Name": "COM4", "405 Shutter-Volts": "0.0000", "Vincent-D3-Port": "COM1", "PRIME-PP 4 MIN ADU AFFECTED": "200", "470 Shutter-MinVolts": "0.0000", "PRIME-PP 3 ENABLED": "Yes", "470 AO-Description": "NI DAC", "PRIME-Description": "PVCAM API device adapter", "520 Shutter-MaxVolts": "5.0000", "520 Shutter-Volts": "0.0000", "528 AO-Sequenceable": "1", "PRIME-Binning": "1x1", "555 Shutter-MaxVolts": "5.0000", "528 Shutter-TriggerSequenceLength": "1000", "405 Shutter-Block voltage": "false", "XLIGHT Spin motor-State": "1", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "Vincent-D3-Time to open (ms)": "35.0000", "Vincent-D3-Time to close (ms)": "35.0000", "445 AO-TriggerSampleRate": "100000", "640 Shutter-IOChannel": "Dev1/ao14", "555 AO-Sequenceable": "1", "520 AO-Name": "AnalogIO", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "470 AO-Block voltage": "false", "COM1-Verbose": "1", "COM9-AnswerTimeout": "10000.0000", "528 Shutter-Name": "AnalogIO", "PRIME-Trigger Timeout (secs)": "10", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "NextFrame": 0, "470 AO-TriggerSequenceLength": "1000", "PRIME-TransposeXY": "0", "445 AO-TriggerSequenceLength": "1000", "COM1-Name": "COM1", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "COM9-DataBits": "8", "COM1-AnswerTimeout": "500.0000", "Camera": "", "TE2000-Version": "V2.50-V2.50-V2.10", "528 Shutter-Volts": "0.0000", "XLIGHT Hub-Name": "XLIGHT Hub", "PIEZO-Description": "Ludl stage driver adapter", "PositionName": "Pos34", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSampleRate": "100000", "PRIME-FanSpeedSetpoint": "High", "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "405 AO-TriggerSampleRate": "100000", "470 Shutter-Block voltage": "false", "470-State": "0", "COM3-Parity": "None", "405 AO-Volts": "0.0000", "PRIME-TransposeMirrorY": "0", "PRIME-ChipName": "GS144BSI", "470 AO-Volts": "0.0000", "PRIME-TransposeMirrorX": "0", "445 Shutter-SupportsTriggering": "0", "470 Shutter-SupportsTriggering": "0", "XLIGHT Hub-Description": "XLIGHT hub", "PRIME-PreampOffLimit": "10000", "470 AO-Sequenceable": "1", "528 Shutter-MaxVolts": "5.0000", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "Core-XYStage": "XYStage", "470 AO-TriggerSampleRate": "100000", "PRIME-Timing-PreTriggerDelayNs": "24672000.0000", "528-DA Device": "528 Shutter", "Focus-Description": "Nikon TE2000 focus adapter", "FilterBlock-State": "5", "520 AO-Demo": "No", "COM3-DelayBetweenCharsMs": "0.0000", "470 Shutter-Volts": "0.0000", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "PRIME-PP 2 THRESHOLD": "125", "PRIME-PP 4 THRESHOLD": "75", "XYStage-Acceleration": "4.0000", "470 Shutter-Demo": "No", "PRIME-Name": "PMPCIECam00", "640-DA Device": "640 Shutter", "405 AO-Block voltage": "false", "ElapsedTime-ms": 1411860, "PRIME-Offset": "100", "445 AO-IOChannel": "Dev1/ao1", "640 AO-MaxVolts": "5.0000", "PositionIndex": 34, "PRIME-PP 1 ENABLED": "Yes", "COM1-BaudRate": "9600", "640-State": "1", "COM3-Verbose": "1", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "LudlController-TransmissionDelay": "0", "PRIME-FullWellCapacity": "62000", "PRIME-SMARTStreamingEnabled": "No", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "640 Shutter-Description": "NI DAC", "528-Description": "DA device that is used as a shutter", "COM4-Description": "Serial port driver (boost:asio)", "405 Shutter-Description": "NI DAC", "TE2000-Name": "TE2000", "528 Shutter-Description": "NI DAC", "COM9-Description": "Serial port driver (boost:asio)", "LudlController-Name": "LudlController", "445 Shutter-TriggerSequenceLength": "1000", "PRIME-SerialNumber": "A18B203008", "445 AO-Volts": "0.0000", "PRIME-X-dimension": "1200", "PRIME-ShutterCloseDelay": "0", "XLIGHT Disk slider-State": "2", "Position": "Default", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 AO-IOChannel": "Dev1/ao5", "520 AO-IOChannel": "Dev1/ao4", "528 AO-TriggerSequenceLength": "1000", "405 Shutter-MaxVolts": "5.0000", "470-DA Device": "470 Shutter", "PRIME-FTCapable": "No", "Core-ChannelGroup": "Channels", "555 AO-SupportsTriggering": "0", "PRIME-Y-dimension": "1200", "Frame": 0, "555 Shutter-Sequenceable": "1", "PRIME-Timing-ExposureTimeNs": "499998000.0000", "Core-ImageProcessor": "", "528-State": "0", "520 AO-Block voltage": "false", "XLIGHT Excitation Wheel-Label": "QUAD", "640 Shutter-Demo": "No", "COM3-StopBits": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "520 Shutter-IOChannel": "Dev1/ao11", "Width": 1200, "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-SupportsTriggering": "0", "640 AO-TriggerSampleRate": "100000", "520-Description": "DA device that is used as a shutter", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "405 Shutter-Sequenceable": "1", "405 Shutter-SupportsTriggering": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-TriggerSampleRate": "100000", "640 Shutter-MaxVolts": "5.0000", "555 AO-MaxVolts": "5.0000", "520 AO-TriggerSequenceLength": "1000", "COM3-BaudRate": "9600", "COM9-BaudRate": "9600", "Vincent-D3-Channel #": "Ch. #1", "PRIME-PMode": "Normal", "OpticalPath-Label": "Left(100)", "BitDepth": 16, "555 AO-TriggerSequenceLength": "1000", "445 Shutter-Description": "NI DAC", "405 AO-Sequenceable": "1", "PRIME-AcquisitionMethod": "Callbacks", "555 AO-Name": "AnalogIO", "PRIME-ShutterMode": "Pre-Exposure", "COM3-Handshaking": "Off", "445 AO-SupportsTriggering": "0", "ROI": "0-0-1200-1200", "COM9-Verbose": "1", "PRIME-Color": "OFF", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "PRIME-ShutterOpenDelay": "0", "COM1-Parity": "None", "Slice": 2, "SlicePosition": -30, "COM9-DelayBetweenCharsMs": "0.0000", "555-Name": "DA Shutter", "PIEZO-LudlSingleAxisName": "B", "XLIGHT Emission Wheel-Label": "725/40 (C)", "640 Shutter-Name": "AnalogIO", "640 AO-Sequenceable": "1", "520 Shutter-Demo": "No", "528 Shutter-Demo": "No", "470-Name": "DA Shutter", "520-DA Device": "520 Shutter", "XYStage-Description": "Ludl XY stage driver adapter", "445 Shutter-MinVolts": "0.0000", "528 AO-TriggerSampleRate": "100000", "405 AO-MinVolts": "0.0000", "405 Shutter-TriggerSequenceLength": "1000", "Height": 1200, "555 AO-Block voltage": "false", "640 Shutter-Volts": "5.0000", "PixelType": "GRAY16", "445 Shutter-Block voltage": "false", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "520 Shutter-TriggerSequenceLength": "1000", "COM9-Name": "COM9", "Core-SLM": "", "555 AO-Volts": "0.0000", "555 Shutter-Block voltage": "false", "XYStage-Speed": "1800.0000", "520 AO-MaxVolts": "5.0000", "528 Shutter-SupportsTriggering": "0", "COM1-Description": "Serial port driver (boost:asio)", "PRIME-MetadataEnabled": "No", "520 Shutter-MinVolts": "0.0000", "470 Shutter-Sequenceable": "1", "PRIME-PreampDelay": "0", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-MinVolts": "0.0000", "ChannelIndex": 3, "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Core-AutoShutter": "1", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "Exposure-ms": 520, "PRIME-PP 2 ENABLED": "Yes", "640 Shutter-TriggerSampleRate": "100000", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "470 Shutter-IOChannel": "Dev1/ao10", "405 AO-Demo": "No", "PixelSizeUm": 1.1, "470 AO-Demo": "No", "470 Shutter-MaxVolts": "5.0000", "Vincent-D3-Name": "Vincent-D3", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FileName": "img_000000000_Il-C_002.tif", "LudlController-Reset": "Operate", "PRIME-Exposure": "520.00", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "445-Name": "DA Shutter", "555 Shutter-Name": "AnalogIO", "520 Shutter-TriggerSampleRate": "100000", "445 Shutter-IOChannel": "Dev1/ao9", "PRIME-TransposeCorrection": "0", "XYStage-StepSize": "0.1000", "445-Description": "DA device that is used as a shutter", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 AO-IOChannel": "Dev1/ao7", "520 Shutter-Name": "AnalogIO", "COM3-DataBits": "8", "640 AO-Volts": "4.5000", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "555 Shutter-IOChannel": "Dev1/ao13", "WaitInterval": null, "520 Shutter-Sequenceable": "1", "YPositionUm": 545.6, "445-State": "0", "445-DA Device": "445 Shutter", "PRIME-CircularBufferAutoSize": "ON", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "555 AO-TriggerSampleRate": "100000", "445 Shutter-Volts": "0.0000", "XLIGHT Hub-Port": "COM3", "Core-AutoFocus": "", "COM4-Handshaking": "Off", "528 Shutter-MinVolts": "0.0000", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "445 Shutter-Name": "AnalogIO", "528 Shutter-Sequenceable": "1", "XYStage-TransposeMirrorX": "0", "XYStage-TransposeMirrorY": "0", "PRIME-ReadoutRate": "100MHz 16bit", "XYStage-Name": "Stage", "640 AO-SupportsTriggering": "0", "640-Name": "DA Shutter", "640 Shutter-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "640 AO-Block voltage": "false", "445 Shutter-Sequenceable": "1", "470 AO-MaxVolts": "5.0000", "COM4-BaudRate": "9600", "470-Description": "DA device that is used as a shutter", "520 Shutter-Description": "NI DAC", "PRIME-CCDTemperature": "-14.8500", "Time": "2019-12-19 09:59:42 -0500", "640 AO-TriggerSequenceLength": "1000", "470 AO-Name": "AnalogIO", "XLIGHT Disk slider-Label": "Disk Pos 40um", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "Binning": "1x1", "TE2000-ModelType": "1", "COM3-AnswerTimeout": "500.0000", "555 Shutter-SupportsTriggering": "0", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "TE2000-Port": "COM4", "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Description": "NI DAC", "COM4-AnswerTimeout": "500.0000", "COM1-DelayBetweenCharsMs": "0.0000", "FilterBlock-Label": "Confocal", "445 AO-Name": "AnalogIO", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-Timing-ReadoutTimeNs": "24672000.0000", "528 AO-Demo": "No", "520 AO-Sequenceable": "1", "555-Description": "DA device that is used as a shutter", "405-DA Device": "405 Shutter", "405 AO-TriggerSequenceLength": "1000", "640 AO-MinVolts": "0.0000", "640 Shutter-Block voltage": "false", "FrameIndex": 0, "PRIME-Port": "CMOS", "555 AO-MinVolts": "0.0000", "COM3-Description": "Serial port driver (boost:asio)", "528 AO-Description": "NI DAC", "555 AO-IOChannel": "Dev1/ao6", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "555 AO-TriggerInputLine": "/Dev1/PFI0", "PRIME-ActualInterval-ms": "390.5200", "LudlController-Port": "COM9", "445 Shutter-Demo": "No", "PRIME-Trigger-Expose Out-Mux": "1", "SliceIndex": 2, "PRIME-CCDTemperatureSetPoint": "-15.0000", "405 Shutter-MinVolts": "0.0000", "PRIME-ExposeOutMode": "First Row", "AxisPositions": null, "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Volts": "0.0000", "528-Name": "DA Shutter", "555-State": "0", "XLIGHT Dichroic-Label": "Di03-R635-t1", "445 AO-Sequenceable": "1", "405-Name": "DA Shutter", "405 AO-IOChannel": "Dev1/ao0", "Core-Camera": "PRIME", "Focus-Name": "Focus", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "405 Shutter-IOChannel": "Dev1/ao8", "640 AO-Demo": "No", "640 Shutter-SupportsTriggering": "0", "445 AO-Demo": "No", "555-DA Device": "555 Shutter", "470 Shutter-Description": "NI DAC", "528 AO-Volts": "0.0000", "COM1-DataBits": "8", "Vincent-D3-Address": "x", "520 AO-SupportsTriggering": "0", "405-Description": "DA device that is used as a shutter", "640 Shutter-MinVolts": "0.0000", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "405 AO-Name": "AnalogIO", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "640 AO-TriggerInputLine": "/Dev1/PFI0", "405 AO-TriggerInputLine": "/Dev1/PFI0", "640-Description": "DA device that is used as a shutter", "445 Shutter-TriggerSampleRate": "100000", "470 Shutter-TriggerSequenceLength": "1000", "COM1-StopBits": "1", "520 Shutter-SupportsTriggering": "0", "405-State": "0", "Core-Initialize": "1", "XLIGHT Emission Wheel-State": "3", "520 AO-Description": "NI DAC", "528 AO-TriggerInputLine": "/Dev1/PFI0", "445 Shutter-MaxVolts": "5.0000", "COM9-Handshaking": "Off", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "XLIGHT Spin motor-Label": "On (spinning)", "PRIME-PP 5 ENABLED": "No", "UUID": "57684176-b6b9-414a-8c6d-d62886948886", "PRIME-TriggerMode": "Internal Trigger", "COM1-Handshaking": "Off", "PRIME-Timing-PostTriggerDelayNs": "49344000.0000", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "Focus-Position": "17741.5000", "528 Shutter-Block voltage": "false", "Channel": "Il-C", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "555 Shutter-Demo": "No", "405 Shutter-Demo": "No", "528 AO-Name": "AnalogIO", "520-State": "0", "Core-Galvo": "", "405 Shutter-TriggerSampleRate": "100000", "Focus-FocusStepSize_(nm)": "100", "PRIME-PixelType": "16bit", "COM4-DataBits": "8", "470 AO-IOChannel": "Dev1/ao2", "520 AO-MinVolts": "0.0000", "COM9-Parity": "None", "PRIME-CircularBufferEnabled": "ON", "PRIME-PP 6 Reset": "No", "640 AO-Description": "NI DAC", "Source": "PRIME", "445 AO-MinVolts": "0.0000", "PIEZO-Name": "Stage", "445 AO-Description": "NI DAC", "PRIME-CircularBufferFrameCount": "46", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "520 AO-Volts": "0.0000", "528 Shutter-TriggerSampleRate": "100000", "XPositionUm": -1324.9, "OpticalPath-State": "4", "PRIME-PP 3 THRESHOLD": "80", "PRIME-PVCAM Version": "3.7.1", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Gain": "1-HDR", "640 Shutter-TriggerSequenceLength": "1000", "528 AO-SupportsTriggering": "0", "445 AO-TriggerInputLine": "/Dev1/PFI0", "OpticalPath-Name": "OpticalPath", "PRIME-PP 4 ENABLED": "Yes", "PRIME-PVCAM Adapter Version": "1.3.29", "COM3-Name": "COM3", "405 AO-MaxVolts": "5.0000", "520 Shutter-Block voltage": "false", "PRIME-Timing-ClearingTimeNs": "49344000.0000", "Core-Focus": "PIEZO", "528 AO-Block voltage": "false", "TE2000-Description": "Nikon TE2000 hub", "528 AO-MinVolts": "0.0000", "COM4-Parity": "None", "Summary": { "Slices": 11, "ChContrastMin": [ 0, 0, 0, 0, 0, 0 ], "PixelAspect": 1, "SlicesFirst": true, "Interval_ms": 0, "Time": "2019-12-19 09:59:21 -0500", "Prefix": "seq_13", "InitialPositionList": [ { "Label": "Pos0", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.21], "Focus": [17741.5], "XYStage": [ 44542.200000000004, 31636.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos1", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.22], "Focus": [17741.4], "XYStage": [ 43801.100000000006, 31637.9 ] }, "GridColumnIndex": 0 }, { "Label": "Pos2", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.5], "XYStage": [ 38561.3, 32040.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos3", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.185], "Focus": [17741.3], "XYStage": [ 37698.4, 32041.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos4", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.16], "Focus": [17741.5], "XYStage": [ 32375.4, 31758.9 ] }, "GridColumnIndex": 0 }, { "Label": "Pos5", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.17000000000002], "Focus": [17741.3], "XYStage": [ 31770.7, 31758.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos6", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.4], "XYStage": [ 26536.800000000003, 31068.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos7", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.205], "Focus": [17741.4], "XYStage": [ 25420.2, 31068.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos8", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.85], "Focus": [17741.3], "XYStage": [ 21059, 31508 ] }, "GridColumnIndex": 0 }, { "Label": "Pos9", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.82], "Focus": [17741.3], "XYStage": [ 19731.9, 31506.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos10", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.79], "Focus": [17741.4], "XYStage": [ 14528.5, 32007.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos11", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.83], "Focus": [17741.2], "XYStage": [ 13830.300000000001, 32009.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos12", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.79], "Focus": [17741.3], "XYStage": [ 8775.300000000001, 32017.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos13", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.815], "Focus": [17741.3], "XYStage": [ 8109.200000000001, 32008.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos14", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.805], "Focus": [17741.3], "XYStage": [ 3483.3, 31908.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos15", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.82], "Focus": [17741.2], "XYStage": [ 2686.8, 31903.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos16", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [211.48000000000002], "Focus": [17741.1], "XYStage": [ -1978.9, 31973.600000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos17", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [211.51500000000001], "Focus": [17741.1], "XYStage": [ -2957.8, 31982.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos18", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.195], "Focus": [17741.5], "XYStage": [ 47747.4, -576.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos19", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.23000000000002], "Focus": [17741.6], "XYStage": [ 47053.9, -576.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos20", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.205], "Focus": [17741.4], "XYStage": [ 41871.200000000004, -479.8 ] }, "GridColumnIndex": 0 }, { "Label": "Pos21", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.4], "XYStage": [ 40882.600000000006, -392 ] }, "GridColumnIndex": 0 }, { "Label": "Pos22", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.24], "Focus": [17741.4], "XYStage": [ 35905.6, -135.3 ] }, "GridColumnIndex": 0 }, { "Label": "Pos23", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.195], "Focus": [17741.5], "XYStage": [ 34990.9, -132.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos24", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.46], "Focus": [17741.4], "XYStage": [ 29678.100000000002, -958.1 ] }, "GridColumnIndex": 0 }, { "Label": "Pos25", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.44], "Focus": [17741.5], "XYStage": [ 28983.600000000002, -939.4000000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos26", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.475], "Focus": [17741.5], "XYStage": [ 23019.7, -122 ] }, "GridColumnIndex": 0 }, { "Label": "Pos27", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.44], "Focus": [17741.7], "XYStage": [ 22428, -116.80000000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos28", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.46], "Focus": [17741.7], "XYStage": [ 16364.900000000001, -181.10000000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos29", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.48000000000002], "Focus": [17741.5], "XYStage": [ 15712.1, -180 ] }, "GridColumnIndex": 0 }, { "Label": "Pos30", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.45000000000002], "Focus": [17741.7], "XYStage": [ 10346.1, 77.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos31", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.49], "Focus": [17741.4], "XYStage": [ 9755.5, 85.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos32", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.475], "Focus": [17741.5], "XYStage": [ 4775.5, 293.90000000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos33", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.445], "Focus": [17741.4], "XYStage": [ 3954.9, 293.90000000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos34", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.455], "Focus": [17741.4], "XYStage": [ -1324.9, 545.6 ] }, "GridColumnIndex": 0 }, { "Label": "Pos35", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.445], "Focus": [17741.3], "XYStage": [ -2097, 576.6 ] }, "GridColumnIndex": 0 } ], "z-step_um": 10, "GridRow": 0, "BitDepth": 16, "Depth": 2, "Channels": 6, "Source": "Micro-Manager", "ChColors": [ -16711681, -256, -65281, -1, -1, -16776961 ], "MetadataVersion": 10, "KeepShutterOpenChannels": true, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536, 65536 ], "CustomIntervals_ms": [], "Height": 1200, "MicroManagerVersion": "1.4.23 20180219", "GridColumn": 0, "UUID": "18808e7c-f2e6-47b3-90d8-1d779ce87dbd", "PixelType": "GRAY16", "Width": 1200, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\Yan\\20191218-YC113-S7S8-miseq", "Frames": 1, "Comment": "", "UserName": "Nikon", "PixelSize_um": 1.1, "ROI": [ 0, 0, 1200, 1200 ], "Date": "2019-12-19", "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC", "P-DAPI-high" ], "KeepShutterOpenSlices": true, "IJType": 1, "ComputerName": "ZadorConfocal2", "Positions": 36, "PositionIndex": 34, "CameraTimeout": 20000, "TimeFirst": false }, "640 AO-Name": "AnalogIO", "Vincent-D3-Command": "Close" }, "FrameKey-0-3-3": { "COM4-StopBits": "1", "528 AO-MaxVolts": "5.0000", "XLIGHT Excitation Wheel-State": "1", "470 Shutter-Name": "AnalogIO", "Core-Shutter": "640", "555 AO-Demo": "No", "XLIGHT Dichroic-State": "2", "XYStage-StepSize-Y": "0.1000", "ZPositionUm": 198.455, "XYStage-StepSize-X": "0.1000", "PRIME-FirmwareVersion": "20.28", "PRIME-ClearCycles": "2", "520-Name": "DA Shutter", "405 Shutter-Name": "AnalogIO", "PIEZO-StepSize": "0.0050", "PRIME-ClearMode": "Pre-Exposure", "470 AO-MinVolts": "0.0000", "405 AO-SupportsTriggering": "1", "CameraChannelIndex": 0, "XYStage-StartSpeed": "500.0000", "COM9-StopBits": "2", "405 AO-Description": "NI DAC", "COM4-DelayBetweenCharsMs": "0.0000", "445 AO-Block voltage": "false", "520 AO-TriggerInputLine": "/Dev1/PFI0", "555 AO-Description": "NI DAC", "520 AO-TriggerSampleRate": "100000", "COM4-Verbose": "1", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM4-Name": "COM4", "405 Shutter-Volts": "0.0000", "Vincent-D3-Port": "COM1", "PRIME-PP 4 MIN ADU AFFECTED": "200", "470 Shutter-MinVolts": "0.0000", "PRIME-PP 3 ENABLED": "Yes", "470 AO-Description": "NI DAC", "PRIME-Description": "PVCAM API device adapter", "520 Shutter-MaxVolts": "5.0000", "520 Shutter-Volts": "0.0000", "528 AO-Sequenceable": "1", "PRIME-Binning": "1x1", "555 Shutter-MaxVolts": "5.0000", "528 Shutter-TriggerSequenceLength": "1000", "405 Shutter-Block voltage": "false", "XLIGHT Spin motor-State": "1", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "Vincent-D3-Time to open (ms)": "35.0000", "Vincent-D3-Time to close (ms)": "35.0000", "445 AO-TriggerSampleRate": "100000", "640 Shutter-IOChannel": "Dev1/ao14", "555 AO-Sequenceable": "1", "520 AO-Name": "AnalogIO", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "470 AO-Block voltage": "false", "COM1-Verbose": "1", "COM9-AnswerTimeout": "10000.0000", "528 Shutter-Name": "AnalogIO", "PRIME-Trigger Timeout (secs)": "10", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "NextFrame": 0, "470 AO-TriggerSequenceLength": "1000", "PRIME-TransposeXY": "0", "445 AO-TriggerSequenceLength": "1000", "COM1-Name": "COM1", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "COM9-DataBits": "8", "COM1-AnswerTimeout": "500.0000", "Camera": "", "TE2000-Version": "V2.50-V2.50-V2.10", "528 Shutter-Volts": "0.0000", "XLIGHT Hub-Name": "XLIGHT Hub", "PIEZO-Description": "Ludl stage driver adapter", "PositionName": "Pos34", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSampleRate": "100000", "PRIME-FanSpeedSetpoint": "High", "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "405 AO-TriggerSampleRate": "100000", "470 Shutter-Block voltage": "false", "470-State": "0", "COM3-Parity": "None", "405 AO-Volts": "0.0000", "PRIME-TransposeMirrorY": "0", "PRIME-ChipName": "GS144BSI", "470 AO-Volts": "0.0000", "PRIME-TransposeMirrorX": "0", "445 Shutter-SupportsTriggering": "0", "470 Shutter-SupportsTriggering": "0", "XLIGHT Hub-Description": "XLIGHT hub", "PRIME-PreampOffLimit": "10000", "470 AO-Sequenceable": "1", "528 Shutter-MaxVolts": "5.0000", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "Core-XYStage": "XYStage", "470 AO-TriggerSampleRate": "100000", "PRIME-Timing-PreTriggerDelayNs": "24672000.0000", "528-DA Device": "528 Shutter", "Focus-Description": "Nikon TE2000 focus adapter", "FilterBlock-State": "5", "520 AO-Demo": "No", "COM3-DelayBetweenCharsMs": "0.0000", "470 Shutter-Volts": "0.0000", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "PRIME-PP 2 THRESHOLD": "125", "PRIME-PP 4 THRESHOLD": "75", "XYStage-Acceleration": "4.0000", "470 Shutter-Demo": "No", "PRIME-Name": "PMPCIECam00", "640-DA Device": "640 Shutter", "405 AO-Block voltage": "false", "ElapsedTime-ms": 1412611, "PRIME-Offset": "100", "445 AO-IOChannel": "Dev1/ao1", "640 AO-MaxVolts": "5.0000", "PositionIndex": 34, "PRIME-PP 1 ENABLED": "Yes", "COM1-BaudRate": "9600", "640-State": "1", "COM3-Verbose": "1", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "LudlController-TransmissionDelay": "0", "PRIME-FullWellCapacity": "62000", "PRIME-SMARTStreamingEnabled": "No", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "640 Shutter-Description": "NI DAC", "528-Description": "DA device that is used as a shutter", "COM4-Description": "Serial port driver (boost:asio)", "405 Shutter-Description": "NI DAC", "TE2000-Name": "TE2000", "528 Shutter-Description": "NI DAC", "COM9-Description": "Serial port driver (boost:asio)", "LudlController-Name": "LudlController", "445 Shutter-TriggerSequenceLength": "1000", "PRIME-SerialNumber": "A18B203008", "445 AO-Volts": "0.0000", "PRIME-X-dimension": "1200", "PRIME-ShutterCloseDelay": "0", "XLIGHT Disk slider-State": "2", "Position": "Default", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 AO-IOChannel": "Dev1/ao5", "520 AO-IOChannel": "Dev1/ao4", "528 AO-TriggerSequenceLength": "1000", "405 Shutter-MaxVolts": "5.0000", "470-DA Device": "470 Shutter", "PRIME-FTCapable": "No", "Core-ChannelGroup": "Channels", "555 AO-SupportsTriggering": "0", "PRIME-Y-dimension": "1200", "Frame": 0, "555 Shutter-Sequenceable": "1", "PRIME-Timing-ExposureTimeNs": "499998000.0000", "Core-ImageProcessor": "", "528-State": "0", "520 AO-Block voltage": "false", "XLIGHT Excitation Wheel-Label": "QUAD", "640 Shutter-Demo": "No", "COM3-StopBits": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "520 Shutter-IOChannel": "Dev1/ao11", "Width": 1200, "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-SupportsTriggering": "0", "640 AO-TriggerSampleRate": "100000", "520-Description": "DA device that is used as a shutter", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "405 Shutter-Sequenceable": "1", "405 Shutter-SupportsTriggering": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-TriggerSampleRate": "100000", "640 Shutter-MaxVolts": "5.0000", "555 AO-MaxVolts": "5.0000", "520 AO-TriggerSequenceLength": "1000", "COM3-BaudRate": "9600", "COM9-BaudRate": "9600", "Vincent-D3-Channel #": "Ch. #1", "PRIME-PMode": "Normal", "OpticalPath-Label": "Left(100)", "BitDepth": 16, "555 AO-TriggerSequenceLength": "1000", "445 Shutter-Description": "NI DAC", "405 AO-Sequenceable": "1", "PRIME-AcquisitionMethod": "Callbacks", "555 AO-Name": "AnalogIO", "PRIME-ShutterMode": "Pre-Exposure", "COM3-Handshaking": "Off", "445 AO-SupportsTriggering": "0", "ROI": "0-0-1200-1200", "COM9-Verbose": "1", "PRIME-Color": "OFF", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "PRIME-ShutterOpenDelay": "0", "COM1-Parity": "None", "Slice": 3, "SlicePosition": -20, "COM9-DelayBetweenCharsMs": "0.0000", "555-Name": "DA Shutter", "PIEZO-LudlSingleAxisName": "B", "XLIGHT Emission Wheel-Label": "725/40 (C)", "640 Shutter-Name": "AnalogIO", "640 AO-Sequenceable": "1", "520 Shutter-Demo": "No", "528 Shutter-Demo": "No", "470-Name": "DA Shutter", "520-DA Device": "520 Shutter", "XYStage-Description": "Ludl XY stage driver adapter", "445 Shutter-MinVolts": "0.0000", "528 AO-TriggerSampleRate": "100000", "405 AO-MinVolts": "0.0000", "405 Shutter-TriggerSequenceLength": "1000", "Height": 1200, "555 AO-Block voltage": "false", "640 Shutter-Volts": "5.0000", "PixelType": "GRAY16", "445 Shutter-Block voltage": "false", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "520 Shutter-TriggerSequenceLength": "1000", "COM9-Name": "COM9", "Core-SLM": "", "555 AO-Volts": "0.0000", "555 Shutter-Block voltage": "false", "XYStage-Speed": "1800.0000", "520 AO-MaxVolts": "5.0000", "528 Shutter-SupportsTriggering": "0", "COM1-Description": "Serial port driver (boost:asio)", "PRIME-MetadataEnabled": "No", "520 Shutter-MinVolts": "0.0000", "470 Shutter-Sequenceable": "1", "PRIME-PreampDelay": "0", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-MinVolts": "0.0000", "ChannelIndex": 3, "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Core-AutoShutter": "1", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "Exposure-ms": 520, "PRIME-PP 2 ENABLED": "Yes", "640 Shutter-TriggerSampleRate": "100000", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "470 Shutter-IOChannel": "Dev1/ao10", "405 AO-Demo": "No", "PixelSizeUm": 1.1, "470 AO-Demo": "No", "470 Shutter-MaxVolts": "5.0000", "Vincent-D3-Name": "Vincent-D3", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FileName": "img_000000000_Il-C_003.tif", "LudlController-Reset": "Operate", "PRIME-Exposure": "520.00", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "445-Name": "DA Shutter", "555 Shutter-Name": "AnalogIO", "520 Shutter-TriggerSampleRate": "100000", "445 Shutter-IOChannel": "Dev1/ao9", "PRIME-TransposeCorrection": "0", "XYStage-StepSize": "0.1000", "445-Description": "DA device that is used as a shutter", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 AO-IOChannel": "Dev1/ao7", "520 Shutter-Name": "AnalogIO", "COM3-DataBits": "8", "640 AO-Volts": "4.5000", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "555 Shutter-IOChannel": "Dev1/ao13", "WaitInterval": null, "520 Shutter-Sequenceable": "1", "YPositionUm": 545.6, "445-State": "0", "445-DA Device": "445 Shutter", "PRIME-CircularBufferAutoSize": "ON", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "555 AO-TriggerSampleRate": "100000", "445 Shutter-Volts": "0.0000", "XLIGHT Hub-Port": "COM3", "Core-AutoFocus": "", "COM4-Handshaking": "Off", "528 Shutter-MinVolts": "0.0000", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "445 Shutter-Name": "AnalogIO", "528 Shutter-Sequenceable": "1", "XYStage-TransposeMirrorX": "0", "XYStage-TransposeMirrorY": "0", "PRIME-ReadoutRate": "100MHz 16bit", "XYStage-Name": "Stage", "640 AO-SupportsTriggering": "0", "640-Name": "DA Shutter", "640 Shutter-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "640 AO-Block voltage": "false", "445 Shutter-Sequenceable": "1", "470 AO-MaxVolts": "5.0000", "COM4-BaudRate": "9600", "470-Description": "DA device that is used as a shutter", "520 Shutter-Description": "NI DAC", "PRIME-CCDTemperature": "-14.8500", "Time": "2019-12-19 09:59:42 -0500", "640 AO-TriggerSequenceLength": "1000", "470 AO-Name": "AnalogIO", "XLIGHT Disk slider-Label": "Disk Pos 40um", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "Binning": "1x1", "TE2000-ModelType": "1", "COM3-AnswerTimeout": "500.0000", "555 Shutter-SupportsTriggering": "0", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "TE2000-Port": "COM4", "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Description": "NI DAC", "COM4-AnswerTimeout": "500.0000", "COM1-DelayBetweenCharsMs": "0.0000", "FilterBlock-Label": "Confocal", "445 AO-Name": "AnalogIO", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-Timing-ReadoutTimeNs": "24672000.0000", "528 AO-Demo": "No", "520 AO-Sequenceable": "1", "555-Description": "DA device that is used as a shutter", "405-DA Device": "405 Shutter", "405 AO-TriggerSequenceLength": "1000", "640 AO-MinVolts": "0.0000", "640 Shutter-Block voltage": "false", "FrameIndex": 0, "PRIME-Port": "CMOS", "555 AO-MinVolts": "0.0000", "COM3-Description": "Serial port driver (boost:asio)", "528 AO-Description": "NI DAC", "555 AO-IOChannel": "Dev1/ao6", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "555 AO-TriggerInputLine": "/Dev1/PFI0", "PRIME-ActualInterval-ms": "390.5200", "LudlController-Port": "COM9", "445 Shutter-Demo": "No", "PRIME-Trigger-Expose Out-Mux": "1", "SliceIndex": 3, "PRIME-CCDTemperatureSetPoint": "-15.0000", "405 Shutter-MinVolts": "0.0000", "PRIME-ExposeOutMode": "First Row", "AxisPositions": null, "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Volts": "0.0000", "528-Name": "DA Shutter", "555-State": "0", "XLIGHT Dichroic-Label": "Di03-R635-t1", "445 AO-Sequenceable": "1", "405-Name": "DA Shutter", "405 AO-IOChannel": "Dev1/ao0", "Core-Camera": "PRIME", "Focus-Name": "Focus", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "405 Shutter-IOChannel": "Dev1/ao8", "640 AO-Demo": "No", "640 Shutter-SupportsTriggering": "0", "445 AO-Demo": "No", "555-DA Device": "555 Shutter", "470 Shutter-Description": "NI DAC", "528 AO-Volts": "0.0000", "COM1-DataBits": "8", "Vincent-D3-Address": "x", "520 AO-SupportsTriggering": "0", "405-Description": "DA device that is used as a shutter", "640 Shutter-MinVolts": "0.0000", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "405 AO-Name": "AnalogIO", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "640 AO-TriggerInputLine": "/Dev1/PFI0", "405 AO-TriggerInputLine": "/Dev1/PFI0", "640-Description": "DA device that is used as a shutter", "445 Shutter-TriggerSampleRate": "100000", "470 Shutter-TriggerSequenceLength": "1000", "COM1-StopBits": "1", "520 Shutter-SupportsTriggering": "0", "405-State": "0", "Core-Initialize": "1", "XLIGHT Emission Wheel-State": "3", "520 AO-Description": "NI DAC", "528 AO-TriggerInputLine": "/Dev1/PFI0", "445 Shutter-MaxVolts": "5.0000", "COM9-Handshaking": "Off", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "XLIGHT Spin motor-Label": "On (spinning)", "PRIME-PP 5 ENABLED": "No", "UUID": "b9da12ea-5fec-4104-ac22-b5868d358b40", "PRIME-TriggerMode": "Internal Trigger", "COM1-Handshaking": "Off", "PRIME-Timing-PostTriggerDelayNs": "49344000.0000", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "Focus-Position": "17741.5000", "528 Shutter-Block voltage": "false", "Channel": "Il-C", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "555 Shutter-Demo": "No", "405 Shutter-Demo": "No", "528 AO-Name": "AnalogIO", "520-State": "0", "Core-Galvo": "", "405 Shutter-TriggerSampleRate": "100000", "Focus-FocusStepSize_(nm)": "100", "PRIME-PixelType": "16bit", "COM4-DataBits": "8", "470 AO-IOChannel": "Dev1/ao2", "520 AO-MinVolts": "0.0000", "COM9-Parity": "None", "PRIME-CircularBufferEnabled": "ON", "PRIME-PP 6 Reset": "No", "640 AO-Description": "NI DAC", "Source": "PRIME", "445 AO-MinVolts": "0.0000", "PIEZO-Name": "Stage", "445 AO-Description": "NI DAC", "PRIME-CircularBufferFrameCount": "46", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "520 AO-Volts": "0.0000", "528 Shutter-TriggerSampleRate": "100000", "XPositionUm": -1324.9, "OpticalPath-State": "4", "PRIME-PP 3 THRESHOLD": "80", "PRIME-PVCAM Version": "3.7.1", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Gain": "1-HDR", "640 Shutter-TriggerSequenceLength": "1000", "528 AO-SupportsTriggering": "0", "445 AO-TriggerInputLine": "/Dev1/PFI0", "OpticalPath-Name": "OpticalPath", "PRIME-PP 4 ENABLED": "Yes", "PRIME-PVCAM Adapter Version": "1.3.29", "COM3-Name": "COM3", "405 AO-MaxVolts": "5.0000", "520 Shutter-Block voltage": "false", "PRIME-Timing-ClearingTimeNs": "49344000.0000", "Core-Focus": "PIEZO", "528 AO-Block voltage": "false", "TE2000-Description": "Nikon TE2000 hub", "528 AO-MinVolts": "0.0000", "COM4-Parity": "None", "Summary": { "Slices": 11, "ChContrastMin": [ 0, 0, 0, 0, 0, 0 ], "PixelAspect": 1, "SlicesFirst": true, "Interval_ms": 0, "Time": "2019-12-19 09:59:21 -0500", "Prefix": "seq_13", "InitialPositionList": [ { "Label": "Pos0", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.21], "Focus": [17741.5], "XYStage": [ 44542.200000000004, 31636.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos1", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.22], "Focus": [17741.4], "XYStage": [ 43801.100000000006, 31637.9 ] }, "GridColumnIndex": 0 }, { "Label": "Pos2", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.5], "XYStage": [ 38561.3, 32040.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos3", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.185], "Focus": [17741.3], "XYStage": [ 37698.4, 32041.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos4", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.16], "Focus": [17741.5], "XYStage": [ 32375.4, 31758.9 ] }, "GridColumnIndex": 0 }, { "Label": "Pos5", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.17000000000002], "Focus": [17741.3], "XYStage": [ 31770.7, 31758.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos6", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.4], "XYStage": [ 26536.800000000003, 31068.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos7", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.205], "Focus": [17741.4], "XYStage": [ 25420.2, 31068.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos8", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.85], "Focus": [17741.3], "XYStage": [ 21059, 31508 ] }, "GridColumnIndex": 0 }, { "Label": "Pos9", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.82], "Focus": [17741.3], "XYStage": [ 19731.9, 31506.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos10", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.79], "Focus": [17741.4], "XYStage": [ 14528.5, 32007.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos11", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.83], "Focus": [17741.2], "XYStage": [ 13830.300000000001, 32009.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos12", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.79], "Focus": [17741.3], "XYStage": [ 8775.300000000001, 32017.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos13", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.815], "Focus": [17741.3], "XYStage": [ 8109.200000000001, 32008.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos14", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.805], "Focus": [17741.3], "XYStage": [ 3483.3, 31908.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos15", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.82], "Focus": [17741.2], "XYStage": [ 2686.8, 31903.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos16", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [211.48000000000002], "Focus": [17741.1], "XYStage": [ -1978.9, 31973.600000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos17", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [211.51500000000001], "Focus": [17741.1], "XYStage": [ -2957.8, 31982.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos18", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.195], "Focus": [17741.5], "XYStage": [ 47747.4, -576.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos19", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.23000000000002], "Focus": [17741.6], "XYStage": [ 47053.9, -576.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos20", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.205], "Focus": [17741.4], "XYStage": [ 41871.200000000004, -479.8 ] }, "GridColumnIndex": 0 }, { "Label": "Pos21", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.4], "XYStage": [ 40882.600000000006, -392 ] }, "GridColumnIndex": 0 }, { "Label": "Pos22", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.24], "Focus": [17741.4], "XYStage": [ 35905.6, -135.3 ] }, "GridColumnIndex": 0 }, { "Label": "Pos23", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.195], "Focus": [17741.5], "XYStage": [ 34990.9, -132.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos24", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.46], "Focus": [17741.4], "XYStage": [ 29678.100000000002, -958.1 ] }, "GridColumnIndex": 0 }, { "Label": "Pos25", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.44], "Focus": [17741.5], "XYStage": [ 28983.600000000002, -939.4000000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos26", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.475], "Focus": [17741.5], "XYStage": [ 23019.7, -122 ] }, "GridColumnIndex": 0 }, { "Label": "Pos27", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.44], "Focus": [17741.7], "XYStage": [ 22428, -116.80000000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos28", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.46], "Focus": [17741.7], "XYStage": [ 16364.900000000001, -181.10000000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos29", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.48000000000002], "Focus": [17741.5], "XYStage": [ 15712.1, -180 ] }, "GridColumnIndex": 0 }, { "Label": "Pos30", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.45000000000002], "Focus": [17741.7], "XYStage": [ 10346.1, 77.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos31", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.49], "Focus": [17741.4], "XYStage": [ 9755.5, 85.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos32", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.475], "Focus": [17741.5], "XYStage": [ 4775.5, 293.90000000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos33", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.445], "Focus": [17741.4], "XYStage": [ 3954.9, 293.90000000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos34", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.455], "Focus": [17741.4], "XYStage": [ -1324.9, 545.6 ] }, "GridColumnIndex": 0 }, { "Label": "Pos35", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.445], "Focus": [17741.3], "XYStage": [ -2097, 576.6 ] }, "GridColumnIndex": 0 } ], "z-step_um": 10, "GridRow": 0, "BitDepth": 16, "Depth": 2, "Channels": 6, "Source": "Micro-Manager", "ChColors": [ -16711681, -256, -65281, -1, -1, -16776961 ], "MetadataVersion": 10, "KeepShutterOpenChannels": true, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536, 65536 ], "CustomIntervals_ms": [], "Height": 1200, "MicroManagerVersion": "1.4.23 20180219", "GridColumn": 0, "UUID": "18808e7c-f2e6-47b3-90d8-1d779ce87dbd", "PixelType": "GRAY16", "Width": 1200, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\Yan\\20191218-YC113-S7S8-miseq", "Frames": 1, "Comment": "", "UserName": "Nikon", "PixelSize_um": 1.1, "ROI": [ 0, 0, 1200, 1200 ], "Date": "2019-12-19", "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC", "P-DAPI-high" ], "KeepShutterOpenSlices": true, "IJType": 1, "ComputerName": "ZadorConfocal2", "Positions": 36, "PositionIndex": 34, "CameraTimeout": 20000, "TimeFirst": false }, "640 AO-Name": "AnalogIO", "Vincent-D3-Command": "Close" }, "FrameKey-0-3-4": { "COM4-StopBits": "1", "528 AO-MaxVolts": "5.0000", "XLIGHT Excitation Wheel-State": "1", "470 Shutter-Name": "AnalogIO", "Core-Shutter": "640", "555 AO-Demo": "No", "XLIGHT Dichroic-State": "2", "XYStage-StepSize-Y": "0.1000", "ZPositionUm": 208.455, "XYStage-StepSize-X": "0.1000", "PRIME-FirmwareVersion": "20.28", "PRIME-ClearCycles": "2", "520-Name": "DA Shutter", "405 Shutter-Name": "AnalogIO", "PIEZO-StepSize": "0.0050", "PRIME-ClearMode": "Pre-Exposure", "470 AO-MinVolts": "0.0000", "405 AO-SupportsTriggering": "1", "CameraChannelIndex": 0, "XYStage-StartSpeed": "500.0000", "COM9-StopBits": "2", "405 AO-Description": "NI DAC", "COM4-DelayBetweenCharsMs": "0.0000", "445 AO-Block voltage": "false", "520 AO-TriggerInputLine": "/Dev1/PFI0", "555 AO-Description": "NI DAC", "520 AO-TriggerSampleRate": "100000", "COM4-Verbose": "1", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM4-Name": "COM4", "405 Shutter-Volts": "0.0000", "Vincent-D3-Port": "COM1", "PRIME-PP 4 MIN ADU AFFECTED": "200", "470 Shutter-MinVolts": "0.0000", "PRIME-PP 3 ENABLED": "Yes", "470 AO-Description": "NI DAC", "PRIME-Description": "PVCAM API device adapter", "520 Shutter-MaxVolts": "5.0000", "520 Shutter-Volts": "0.0000", "528 AO-Sequenceable": "1", "PRIME-Binning": "1x1", "555 Shutter-MaxVolts": "5.0000", "528 Shutter-TriggerSequenceLength": "1000", "405 Shutter-Block voltage": "false", "XLIGHT Spin motor-State": "1", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "Vincent-D3-Time to open (ms)": "35.0000", "Vincent-D3-Time to close (ms)": "35.0000", "445 AO-TriggerSampleRate": "100000", "640 Shutter-IOChannel": "Dev1/ao14", "555 AO-Sequenceable": "1", "520 AO-Name": "AnalogIO", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "470 AO-Block voltage": "false", "COM1-Verbose": "1", "COM9-AnswerTimeout": "10000.0000", "528 Shutter-Name": "AnalogIO", "PRIME-Trigger Timeout (secs)": "10", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "NextFrame": 0, "470 AO-TriggerSequenceLength": "1000", "PRIME-TransposeXY": "0", "445 AO-TriggerSequenceLength": "1000", "COM1-Name": "COM1", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "COM9-DataBits": "8", "COM1-AnswerTimeout": "500.0000", "Camera": "", "TE2000-Version": "V2.50-V2.50-V2.10", "528 Shutter-Volts": "0.0000", "XLIGHT Hub-Name": "XLIGHT Hub", "PIEZO-Description": "Ludl stage driver adapter", "PositionName": "Pos34", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSampleRate": "100000", "PRIME-FanSpeedSetpoint": "High", "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "405 AO-TriggerSampleRate": "100000", "470 Shutter-Block voltage": "false", "470-State": "0", "COM3-Parity": "None", "405 AO-Volts": "0.0000", "PRIME-TransposeMirrorY": "0", "PRIME-ChipName": "GS144BSI", "470 AO-Volts": "0.0000", "PRIME-TransposeMirrorX": "0", "445 Shutter-SupportsTriggering": "0", "470 Shutter-SupportsTriggering": "0", "XLIGHT Hub-Description": "XLIGHT hub", "PRIME-PreampOffLimit": "10000", "470 AO-Sequenceable": "1", "528 Shutter-MaxVolts": "5.0000", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "Core-XYStage": "XYStage", "470 AO-TriggerSampleRate": "100000", "PRIME-Timing-PreTriggerDelayNs": "24672000.0000", "528-DA Device": "528 Shutter", "Focus-Description": "Nikon TE2000 focus adapter", "FilterBlock-State": "5", "520 AO-Demo": "No", "COM3-DelayBetweenCharsMs": "0.0000", "470 Shutter-Volts": "0.0000", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "PRIME-PP 2 THRESHOLD": "125", "PRIME-PP 4 THRESHOLD": "75", "XYStage-Acceleration": "4.0000", "470 Shutter-Demo": "No", "PRIME-Name": "PMPCIECam00", "640-DA Device": "640 Shutter", "405 AO-Block voltage": "false", "ElapsedTime-ms": 1413489, "PRIME-Offset": "100", "445 AO-IOChannel": "Dev1/ao1", "640 AO-MaxVolts": "5.0000", "PositionIndex": 34, "PRIME-PP 1 ENABLED": "Yes", "COM1-BaudRate": "9600", "640-State": "1", "COM3-Verbose": "1", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "LudlController-TransmissionDelay": "0", "PRIME-FullWellCapacity": "62000", "PRIME-SMARTStreamingEnabled": "No", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "640 Shutter-Description": "NI DAC", "528-Description": "DA device that is used as a shutter", "COM4-Description": "Serial port driver (boost:asio)", "405 Shutter-Description": "NI DAC", "TE2000-Name": "TE2000", "528 Shutter-Description": "NI DAC", "COM9-Description": "Serial port driver (boost:asio)", "LudlController-Name": "LudlController", "445 Shutter-TriggerSequenceLength": "1000", "PRIME-SerialNumber": "A18B203008", "445 AO-Volts": "0.0000", "PRIME-X-dimension": "1200", "PRIME-ShutterCloseDelay": "0", "XLIGHT Disk slider-State": "2", "Position": "Default", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 AO-IOChannel": "Dev1/ao5", "520 AO-IOChannel": "Dev1/ao4", "528 AO-TriggerSequenceLength": "1000", "405 Shutter-MaxVolts": "5.0000", "470-DA Device": "470 Shutter", "PRIME-FTCapable": "No", "Core-ChannelGroup": "Channels", "555 AO-SupportsTriggering": "0", "PRIME-Y-dimension": "1200", "Frame": 0, "555 Shutter-Sequenceable": "1", "PRIME-Timing-ExposureTimeNs": "499998000.0000", "Core-ImageProcessor": "", "528-State": "0", "520 AO-Block voltage": "false", "XLIGHT Excitation Wheel-Label": "QUAD", "640 Shutter-Demo": "No", "COM3-StopBits": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "520 Shutter-IOChannel": "Dev1/ao11", "Width": 1200, "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-SupportsTriggering": "0", "640 AO-TriggerSampleRate": "100000", "520-Description": "DA device that is used as a shutter", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "405 Shutter-Sequenceable": "1", "405 Shutter-SupportsTriggering": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-TriggerSampleRate": "100000", "640 Shutter-MaxVolts": "5.0000", "555 AO-MaxVolts": "5.0000", "520 AO-TriggerSequenceLength": "1000", "COM3-BaudRate": "9600", "COM9-BaudRate": "9600", "Vincent-D3-Channel #": "Ch. #1", "PRIME-PMode": "Normal", "OpticalPath-Label": "Left(100)", "BitDepth": 16, "555 AO-TriggerSequenceLength": "1000", "445 Shutter-Description": "NI DAC", "405 AO-Sequenceable": "1", "PRIME-AcquisitionMethod": "Callbacks", "555 AO-Name": "AnalogIO", "PRIME-ShutterMode": "Pre-Exposure", "COM3-Handshaking": "Off", "445 AO-SupportsTriggering": "0", "ROI": "0-0-1200-1200", "COM9-Verbose": "1", "PRIME-Color": "OFF", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "PRIME-ShutterOpenDelay": "0", "COM1-Parity": "None", "Slice": 4, "SlicePosition": -10, "COM9-DelayBetweenCharsMs": "0.0000", "555-Name": "DA Shutter", "PIEZO-LudlSingleAxisName": "B", "XLIGHT Emission Wheel-Label": "725/40 (C)", "640 Shutter-Name": "AnalogIO", "640 AO-Sequenceable": "1", "520 Shutter-Demo": "No", "528 Shutter-Demo": "No", "470-Name": "DA Shutter", "520-DA Device": "520 Shutter", "XYStage-Description": "Ludl XY stage driver adapter", "445 Shutter-MinVolts": "0.0000", "528 AO-TriggerSampleRate": "100000", "405 AO-MinVolts": "0.0000", "405 Shutter-TriggerSequenceLength": "1000", "Height": 1200, "555 AO-Block voltage": "false", "640 Shutter-Volts": "5.0000", "PixelType": "GRAY16", "445 Shutter-Block voltage": "false", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "520 Shutter-TriggerSequenceLength": "1000", "COM9-Name": "COM9", "Core-SLM": "", "555 AO-Volts": "0.0000", "555 Shutter-Block voltage": "false", "XYStage-Speed": "1800.0000", "520 AO-MaxVolts": "5.0000", "528 Shutter-SupportsTriggering": "0", "COM1-Description": "Serial port driver (boost:asio)", "PRIME-MetadataEnabled": "No", "520 Shutter-MinVolts": "0.0000", "470 Shutter-Sequenceable": "1", "PRIME-PreampDelay": "0", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-MinVolts": "0.0000", "ChannelIndex": 3, "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Core-AutoShutter": "1", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "Exposure-ms": 520, "PRIME-PP 2 ENABLED": "Yes", "640 Shutter-TriggerSampleRate": "100000", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "470 Shutter-IOChannel": "Dev1/ao10", "405 AO-Demo": "No", "PixelSizeUm": 1.1, "470 AO-Demo": "No", "470 Shutter-MaxVolts": "5.0000", "Vincent-D3-Name": "Vincent-D3", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FileName": "img_000000000_Il-C_004.tif", "LudlController-Reset": "Operate", "PRIME-Exposure": "520.00", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "445-Name": "DA Shutter", "555 Shutter-Name": "AnalogIO", "520 Shutter-TriggerSampleRate": "100000", "445 Shutter-IOChannel": "Dev1/ao9", "PRIME-TransposeCorrection": "0", "XYStage-StepSize": "0.1000", "445-Description": "DA device that is used as a shutter", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 AO-IOChannel": "Dev1/ao7", "520 Shutter-Name": "AnalogIO", "COM3-DataBits": "8", "640 AO-Volts": "4.5000", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "555 Shutter-IOChannel": "Dev1/ao13", "WaitInterval": null, "520 Shutter-Sequenceable": "1", "YPositionUm": 545.6, "445-State": "0", "445-DA Device": "445 Shutter", "PRIME-CircularBufferAutoSize": "ON", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "555 AO-TriggerSampleRate": "100000", "445 Shutter-Volts": "0.0000", "XLIGHT Hub-Port": "COM3", "Core-AutoFocus": "", "COM4-Handshaking": "Off", "528 Shutter-MinVolts": "0.0000", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "445 Shutter-Name": "AnalogIO", "528 Shutter-Sequenceable": "1", "XYStage-TransposeMirrorX": "0", "XYStage-TransposeMirrorY": "0", "PRIME-ReadoutRate": "100MHz 16bit", "XYStage-Name": "Stage", "640 AO-SupportsTriggering": "0", "640-Name": "DA Shutter", "640 Shutter-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "640 AO-Block voltage": "false", "445 Shutter-Sequenceable": "1", "470 AO-MaxVolts": "5.0000", "COM4-BaudRate": "9600", "470-Description": "DA device that is used as a shutter", "520 Shutter-Description": "NI DAC", "PRIME-CCDTemperature": "-14.8500", "Time": "2019-12-19 09:59:43 -0500", "640 AO-TriggerSequenceLength": "1000", "470 AO-Name": "AnalogIO", "XLIGHT Disk slider-Label": "Disk Pos 40um", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "Binning": "1x1", "TE2000-ModelType": "1", "COM3-AnswerTimeout": "500.0000", "555 Shutter-SupportsTriggering": "0", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "TE2000-Port": "COM4", "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Description": "NI DAC", "COM4-AnswerTimeout": "500.0000", "COM1-DelayBetweenCharsMs": "0.0000", "FilterBlock-Label": "Confocal", "445 AO-Name": "AnalogIO", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-Timing-ReadoutTimeNs": "24672000.0000", "528 AO-Demo": "No", "520 AO-Sequenceable": "1", "555-Description": "DA device that is used as a shutter", "405-DA Device": "405 Shutter", "405 AO-TriggerSequenceLength": "1000", "640 AO-MinVolts": "0.0000", "640 Shutter-Block voltage": "false", "FrameIndex": 0, "PRIME-Port": "CMOS", "555 AO-MinVolts": "0.0000", "COM3-Description": "Serial port driver (boost:asio)", "528 AO-Description": "NI DAC", "555 AO-IOChannel": "Dev1/ao6", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "555 AO-TriggerInputLine": "/Dev1/PFI0", "PRIME-ActualInterval-ms": "390.5200", "LudlController-Port": "COM9", "445 Shutter-Demo": "No", "PRIME-Trigger-Expose Out-Mux": "1", "SliceIndex": 4, "PRIME-CCDTemperatureSetPoint": "-15.0000", "405 Shutter-MinVolts": "0.0000", "PRIME-ExposeOutMode": "First Row", "AxisPositions": null, "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Volts": "0.0000", "528-Name": "DA Shutter", "555-State": "0", "XLIGHT Dichroic-Label": "Di03-R635-t1", "445 AO-Sequenceable": "1", "405-Name": "DA Shutter", "405 AO-IOChannel": "Dev1/ao0", "Core-Camera": "PRIME", "Focus-Name": "Focus", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "405 Shutter-IOChannel": "Dev1/ao8", "640 AO-Demo": "No", "640 Shutter-SupportsTriggering": "0", "445 AO-Demo": "No", "555-DA Device": "555 Shutter", "470 Shutter-Description": "NI DAC", "528 AO-Volts": "0.0000", "COM1-DataBits": "8", "Vincent-D3-Address": "x", "520 AO-SupportsTriggering": "0", "405-Description": "DA device that is used as a shutter", "640 Shutter-MinVolts": "0.0000", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "405 AO-Name": "AnalogIO", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "640 AO-TriggerInputLine": "/Dev1/PFI0", "405 AO-TriggerInputLine": "/Dev1/PFI0", "640-Description": "DA device that is used as a shutter", "445 Shutter-TriggerSampleRate": "100000", "470 Shutter-TriggerSequenceLength": "1000", "COM1-StopBits": "1", "520 Shutter-SupportsTriggering": "0", "405-State": "0", "Core-Initialize": "1", "XLIGHT Emission Wheel-State": "3", "520 AO-Description": "NI DAC", "528 AO-TriggerInputLine": "/Dev1/PFI0", "445 Shutter-MaxVolts": "5.0000", "COM9-Handshaking": "Off", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "XLIGHT Spin motor-Label": "On (spinning)", "PRIME-PP 5 ENABLED": "No", "UUID": "31167a66-d8c0-430c-83ec-99fdf1b68c2a", "PRIME-TriggerMode": "Internal Trigger", "COM1-Handshaking": "Off", "PRIME-Timing-PostTriggerDelayNs": "49344000.0000", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "Focus-Position": "17741.5000", "528 Shutter-Block voltage": "false", "Channel": "Il-C", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "555 Shutter-Demo": "No", "405 Shutter-Demo": "No", "528 AO-Name": "AnalogIO", "520-State": "0", "Core-Galvo": "", "405 Shutter-TriggerSampleRate": "100000", "Focus-FocusStepSize_(nm)": "100", "PRIME-PixelType": "16bit", "COM4-DataBits": "8", "470 AO-IOChannel": "Dev1/ao2", "520 AO-MinVolts": "0.0000", "COM9-Parity": "None", "PRIME-CircularBufferEnabled": "ON", "PRIME-PP 6 Reset": "No", "640 AO-Description": "NI DAC", "Source": "PRIME", "445 AO-MinVolts": "0.0000", "PIEZO-Name": "Stage", "445 AO-Description": "NI DAC", "PRIME-CircularBufferFrameCount": "46", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "520 AO-Volts": "0.0000", "528 Shutter-TriggerSampleRate": "100000", "XPositionUm": -1324.9, "OpticalPath-State": "4", "PRIME-PP 3 THRESHOLD": "80", "PRIME-PVCAM Version": "3.7.1", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Gain": "1-HDR", "640 Shutter-TriggerSequenceLength": "1000", "528 AO-SupportsTriggering": "0", "445 AO-TriggerInputLine": "/Dev1/PFI0", "OpticalPath-Name": "OpticalPath", "PRIME-PP 4 ENABLED": "Yes", "PRIME-PVCAM Adapter Version": "1.3.29", "COM3-Name": "COM3", "405 AO-MaxVolts": "5.0000", "520 Shutter-Block voltage": "false", "PRIME-Timing-ClearingTimeNs": "49344000.0000", "Core-Focus": "PIEZO", "528 AO-Block voltage": "false", "TE2000-Description": "Nikon TE2000 hub", "528 AO-MinVolts": "0.0000", "COM4-Parity": "None", "Summary": { "Slices": 11, "ChContrastMin": [ 0, 0, 0, 0, 0, 0 ], "PixelAspect": 1, "SlicesFirst": true, "Interval_ms": 0, "Time": "2019-12-19 09:59:21 -0500", "Prefix": "seq_13", "InitialPositionList": [ { "Label": "Pos0", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.21], "Focus": [17741.5], "XYStage": [ 44542.200000000004, 31636.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos1", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.22], "Focus": [17741.4], "XYStage": [ 43801.100000000006, 31637.9 ] }, "GridColumnIndex": 0 }, { "Label": "Pos2", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.5], "XYStage": [ 38561.3, 32040.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos3", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.185], "Focus": [17741.3], "XYStage": [ 37698.4, 32041.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos4", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.16], "Focus": [17741.5], "XYStage": [ 32375.4, 31758.9 ] }, "GridColumnIndex": 0 }, { "Label": "Pos5", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.17000000000002], "Focus": [17741.3], "XYStage": [ 31770.7, 31758.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos6", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.4], "XYStage": [ 26536.800000000003, 31068.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos7", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.205], "Focus": [17741.4], "XYStage": [ 25420.2, 31068.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos8", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.85], "Focus": [17741.3], "XYStage": [ 21059, 31508 ] }, "GridColumnIndex": 0 }, { "Label": "Pos9", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.82], "Focus": [17741.3], "XYStage": [ 19731.9, 31506.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos10", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.79], "Focus": [17741.4], "XYStage": [ 14528.5, 32007.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos11", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.83], "Focus": [17741.2], "XYStage": [ 13830.300000000001, 32009.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos12", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.79], "Focus": [17741.3], "XYStage": [ 8775.300000000001, 32017.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos13", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.815], "Focus": [17741.3], "XYStage": [ 8109.200000000001, 32008.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos14", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.805], "Focus": [17741.3], "XYStage": [ 3483.3, 31908.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos15", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.82], "Focus": [17741.2], "XYStage": [ 2686.8, 31903.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos16", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [211.48000000000002], "Focus": [17741.1], "XYStage": [ -1978.9, 31973.600000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos17", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [211.51500000000001], "Focus": [17741.1], "XYStage": [ -2957.8, 31982.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos18", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.195], "Focus": [17741.5], "XYStage": [ 47747.4, -576.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos19", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.23000000000002], "Focus": [17741.6], "XYStage": [ 47053.9, -576.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos20", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.205], "Focus": [17741.4], "XYStage": [ 41871.200000000004, -479.8 ] }, "GridColumnIndex": 0 }, { "Label": "Pos21", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.4], "XYStage": [ 40882.600000000006, -392 ] }, "GridColumnIndex": 0 }, { "Label": "Pos22", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.24], "Focus": [17741.4], "XYStage": [ 35905.6, -135.3 ] }, "GridColumnIndex": 0 }, { "Label": "Pos23", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.195], "Focus": [17741.5], "XYStage": [ 34990.9, -132.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos24", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.46], "Focus": [17741.4], "XYStage": [ 29678.100000000002, -958.1 ] }, "GridColumnIndex": 0 }, { "Label": "Pos25", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.44], "Focus": [17741.5], "XYStage": [ 28983.600000000002, -939.4000000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos26", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.475], "Focus": [17741.5], "XYStage": [ 23019.7, -122 ] }, "GridColumnIndex": 0 }, { "Label": "Pos27", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.44], "Focus": [17741.7], "XYStage": [ 22428, -116.80000000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos28", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.46], "Focus": [17741.7], "XYStage": [ 16364.900000000001, -181.10000000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos29", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.48000000000002], "Focus": [17741.5], "XYStage": [ 15712.1, -180 ] }, "GridColumnIndex": 0 }, { "Label": "Pos30", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.45000000000002], "Focus": [17741.7], "XYStage": [ 10346.1, 77.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos31", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.49], "Focus": [17741.4], "XYStage": [ 9755.5, 85.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos32", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.475], "Focus": [17741.5], "XYStage": [ 4775.5, 293.90000000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos33", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.445], "Focus": [17741.4], "XYStage": [ 3954.9, 293.90000000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos34", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.455], "Focus": [17741.4], "XYStage": [ -1324.9, 545.6 ] }, "GridColumnIndex": 0 }, { "Label": "Pos35", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.445], "Focus": [17741.3], "XYStage": [ -2097, 576.6 ] }, "GridColumnIndex": 0 } ], "z-step_um": 10, "GridRow": 0, "BitDepth": 16, "Depth": 2, "Channels": 6, "Source": "Micro-Manager", "ChColors": [ -16711681, -256, -65281, -1, -1, -16776961 ], "MetadataVersion": 10, "KeepShutterOpenChannels": true, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536, 65536 ], "CustomIntervals_ms": [], "Height": 1200, "MicroManagerVersion": "1.4.23 20180219", "GridColumn": 0, "UUID": "18808e7c-f2e6-47b3-90d8-1d779ce87dbd", "PixelType": "GRAY16", "Width": 1200, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\Yan\\20191218-YC113-S7S8-miseq", "Frames": 1, "Comment": "", "UserName": "Nikon", "PixelSize_um": 1.1, "ROI": [ 0, 0, 1200, 1200 ], "Date": "2019-12-19", "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC", "P-DAPI-high" ], "KeepShutterOpenSlices": true, "IJType": 1, "ComputerName": "ZadorConfocal2", "Positions": 36, "PositionIndex": 34, "CameraTimeout": 20000, "TimeFirst": false }, "640 AO-Name": "AnalogIO", "Vincent-D3-Command": "Close" }, "FrameKey-0-3-5": { "COM4-StopBits": "1", "528 AO-MaxVolts": "5.0000", "XLIGHT Excitation Wheel-State": "1", "470 Shutter-Name": "AnalogIO", "Core-Shutter": "640", "555 AO-Demo": "No", "XLIGHT Dichroic-State": "2", "XYStage-StepSize-Y": "0.1000", "ZPositionUm": 218.455, "XYStage-StepSize-X": "0.1000", "PRIME-FirmwareVersion": "20.28", "PRIME-ClearCycles": "2", "520-Name": "DA Shutter", "405 Shutter-Name": "AnalogIO", "PIEZO-StepSize": "0.0050", "PRIME-ClearMode": "Pre-Exposure", "470 AO-MinVolts": "0.0000", "405 AO-SupportsTriggering": "1", "CameraChannelIndex": 0, "XYStage-StartSpeed": "500.0000", "COM9-StopBits": "2", "405 AO-Description": "NI DAC", "COM4-DelayBetweenCharsMs": "0.0000", "445 AO-Block voltage": "false", "520 AO-TriggerInputLine": "/Dev1/PFI0", "555 AO-Description": "NI DAC", "520 AO-TriggerSampleRate": "100000", "COM4-Verbose": "1", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM4-Name": "COM4", "405 Shutter-Volts": "0.0000", "Vincent-D3-Port": "COM1", "PRIME-PP 4 MIN ADU AFFECTED": "200", "470 Shutter-MinVolts": "0.0000", "PRIME-PP 3 ENABLED": "Yes", "470 AO-Description": "NI DAC", "PRIME-Description": "PVCAM API device adapter", "520 Shutter-MaxVolts": "5.0000", "520 Shutter-Volts": "0.0000", "528 AO-Sequenceable": "1", "PRIME-Binning": "1x1", "555 Shutter-MaxVolts": "5.0000", "528 Shutter-TriggerSequenceLength": "1000", "405 Shutter-Block voltage": "false", "XLIGHT Spin motor-State": "1", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "Vincent-D3-Time to open (ms)": "35.0000", "Vincent-D3-Time to close (ms)": "35.0000", "445 AO-TriggerSampleRate": "100000", "640 Shutter-IOChannel": "Dev1/ao14", "555 AO-Sequenceable": "1", "520 AO-Name": "AnalogIO", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "470 AO-Block voltage": "false", "COM1-Verbose": "1", "COM9-AnswerTimeout": "10000.0000", "528 Shutter-Name": "AnalogIO", "PRIME-Trigger Timeout (secs)": "10", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "NextFrame": 0, "470 AO-TriggerSequenceLength": "1000", "PRIME-TransposeXY": "0", "445 AO-TriggerSequenceLength": "1000", "COM1-Name": "COM1", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "COM9-DataBits": "8", "COM1-AnswerTimeout": "500.0000", "Camera": "", "TE2000-Version": "V2.50-V2.50-V2.10", "528 Shutter-Volts": "0.0000", "XLIGHT Hub-Name": "XLIGHT Hub", "PIEZO-Description": "Ludl stage driver adapter", "PositionName": "Pos34", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSampleRate": "100000", "PRIME-FanSpeedSetpoint": "High", "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "405 AO-TriggerSampleRate": "100000", "470 Shutter-Block voltage": "false", "470-State": "0", "COM3-Parity": "None", "405 AO-Volts": "0.0000", "PRIME-TransposeMirrorY": "0", "PRIME-ChipName": "GS144BSI", "470 AO-Volts": "0.0000", "PRIME-TransposeMirrorX": "0", "445 Shutter-SupportsTriggering": "0", "470 Shutter-SupportsTriggering": "0", "XLIGHT Hub-Description": "XLIGHT hub", "PRIME-PreampOffLimit": "10000", "470 AO-Sequenceable": "1", "528 Shutter-MaxVolts": "5.0000", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "Core-XYStage": "XYStage", "470 AO-TriggerSampleRate": "100000", "PRIME-Timing-PreTriggerDelayNs": "24672000.0000", "528-DA Device": "528 Shutter", "Focus-Description": "Nikon TE2000 focus adapter", "FilterBlock-State": "5", "520 AO-Demo": "No", "COM3-DelayBetweenCharsMs": "0.0000", "470 Shutter-Volts": "0.0000", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "PRIME-PP 2 THRESHOLD": "125", "PRIME-PP 4 THRESHOLD": "75", "XYStage-Acceleration": "4.0000", "470 Shutter-Demo": "No", "PRIME-Name": "PMPCIECam00", "640-DA Device": "640 Shutter", "405 AO-Block voltage": "false", "ElapsedTime-ms": 1414242, "PRIME-Offset": "100", "445 AO-IOChannel": "Dev1/ao1", "640 AO-MaxVolts": "5.0000", "PositionIndex": 34, "PRIME-PP 1 ENABLED": "Yes", "COM1-BaudRate": "9600", "640-State": "1", "COM3-Verbose": "1", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "LudlController-TransmissionDelay": "0", "PRIME-FullWellCapacity": "62000", "PRIME-SMARTStreamingEnabled": "No", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "640 Shutter-Description": "NI DAC", "528-Description": "DA device that is used as a shutter", "COM4-Description": "Serial port driver (boost:asio)", "405 Shutter-Description": "NI DAC", "TE2000-Name": "TE2000", "528 Shutter-Description": "NI DAC", "COM9-Description": "Serial port driver (boost:asio)", "LudlController-Name": "LudlController", "445 Shutter-TriggerSequenceLength": "1000", "PRIME-SerialNumber": "A18B203008", "445 AO-Volts": "0.0000", "PRIME-X-dimension": "1200", "PRIME-ShutterCloseDelay": "0", "XLIGHT Disk slider-State": "2", "Position": "Default", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 AO-IOChannel": "Dev1/ao5", "520 AO-IOChannel": "Dev1/ao4", "528 AO-TriggerSequenceLength": "1000", "405 Shutter-MaxVolts": "5.0000", "470-DA Device": "470 Shutter", "PRIME-FTCapable": "No", "Core-ChannelGroup": "Channels", "555 AO-SupportsTriggering": "0", "PRIME-Y-dimension": "1200", "Frame": 0, "555 Shutter-Sequenceable": "1", "PRIME-Timing-ExposureTimeNs": "499998000.0000", "Core-ImageProcessor": "", "528-State": "0", "520 AO-Block voltage": "false", "XLIGHT Excitation Wheel-Label": "QUAD", "640 Shutter-Demo": "No", "COM3-StopBits": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "520 Shutter-IOChannel": "Dev1/ao11", "Width": 1200, "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-SupportsTriggering": "0", "640 AO-TriggerSampleRate": "100000", "520-Description": "DA device that is used as a shutter", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "405 Shutter-Sequenceable": "1", "405 Shutter-SupportsTriggering": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-TriggerSampleRate": "100000", "640 Shutter-MaxVolts": "5.0000", "555 AO-MaxVolts": "5.0000", "520 AO-TriggerSequenceLength": "1000", "COM3-BaudRate": "9600", "COM9-BaudRate": "9600", "Vincent-D3-Channel #": "Ch. #1", "PRIME-PMode": "Normal", "OpticalPath-Label": "Left(100)", "BitDepth": 16, "555 AO-TriggerSequenceLength": "1000", "445 Shutter-Description": "NI DAC", "405 AO-Sequenceable": "1", "PRIME-AcquisitionMethod": "Callbacks", "555 AO-Name": "AnalogIO", "PRIME-ShutterMode": "Pre-Exposure", "COM3-Handshaking": "Off", "445 AO-SupportsTriggering": "0", "ROI": "0-0-1200-1200", "COM9-Verbose": "1", "PRIME-Color": "OFF", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "PRIME-ShutterOpenDelay": "0", "COM1-Parity": "None", "Slice": 5, "SlicePosition": 0, "COM9-DelayBetweenCharsMs": "0.0000", "555-Name": "DA Shutter", "PIEZO-LudlSingleAxisName": "B", "XLIGHT Emission Wheel-Label": "725/40 (C)", "640 Shutter-Name": "AnalogIO", "640 AO-Sequenceable": "1", "520 Shutter-Demo": "No", "528 Shutter-Demo": "No", "470-Name": "DA Shutter", "520-DA Device": "520 Shutter", "XYStage-Description": "Ludl XY stage driver adapter", "445 Shutter-MinVolts": "0.0000", "528 AO-TriggerSampleRate": "100000", "405 AO-MinVolts": "0.0000", "405 Shutter-TriggerSequenceLength": "1000", "Height": 1200, "555 AO-Block voltage": "false", "640 Shutter-Volts": "5.0000", "PixelType": "GRAY16", "445 Shutter-Block voltage": "false", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "520 Shutter-TriggerSequenceLength": "1000", "COM9-Name": "COM9", "Core-SLM": "", "555 AO-Volts": "0.0000", "555 Shutter-Block voltage": "false", "XYStage-Speed": "1800.0000", "520 AO-MaxVolts": "5.0000", "528 Shutter-SupportsTriggering": "0", "COM1-Description": "Serial port driver (boost:asio)", "PRIME-MetadataEnabled": "No", "520 Shutter-MinVolts": "0.0000", "470 Shutter-Sequenceable": "1", "PRIME-PreampDelay": "0", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-MinVolts": "0.0000", "ChannelIndex": 3, "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Core-AutoShutter": "1", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "Exposure-ms": 520, "PRIME-PP 2 ENABLED": "Yes", "640 Shutter-TriggerSampleRate": "100000", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "470 Shutter-IOChannel": "Dev1/ao10", "405 AO-Demo": "No", "PixelSizeUm": 1.1, "470 AO-Demo": "No", "470 Shutter-MaxVolts": "5.0000", "Vincent-D3-Name": "Vincent-D3", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FileName": "img_000000000_Il-C_005.tif", "LudlController-Reset": "Operate", "PRIME-Exposure": "520.00", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "445-Name": "DA Shutter", "555 Shutter-Name": "AnalogIO", "520 Shutter-TriggerSampleRate": "100000", "445 Shutter-IOChannel": "Dev1/ao9", "PRIME-TransposeCorrection": "0", "XYStage-StepSize": "0.1000", "445-Description": "DA device that is used as a shutter", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 AO-IOChannel": "Dev1/ao7", "520 Shutter-Name": "AnalogIO", "COM3-DataBits": "8", "640 AO-Volts": "4.5000", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "555 Shutter-IOChannel": "Dev1/ao13", "WaitInterval": null, "520 Shutter-Sequenceable": "1", "YPositionUm": 545.6, "445-State": "0", "445-DA Device": "445 Shutter", "PRIME-CircularBufferAutoSize": "ON", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "555 AO-TriggerSampleRate": "100000", "445 Shutter-Volts": "0.0000", "XLIGHT Hub-Port": "COM3", "Core-AutoFocus": "", "COM4-Handshaking": "Off", "528 Shutter-MinVolts": "0.0000", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "445 Shutter-Name": "AnalogIO", "528 Shutter-Sequenceable": "1", "XYStage-TransposeMirrorX": "0", "XYStage-TransposeMirrorY": "0", "PRIME-ReadoutRate": "100MHz 16bit", "XYStage-Name": "Stage", "640 AO-SupportsTriggering": "0", "640-Name": "DA Shutter", "640 Shutter-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "640 AO-Block voltage": "false", "445 Shutter-Sequenceable": "1", "470 AO-MaxVolts": "5.0000", "COM4-BaudRate": "9600", "470-Description": "DA device that is used as a shutter", "520 Shutter-Description": "NI DAC", "PRIME-CCDTemperature": "-14.8500", "Time": "2019-12-19 09:59:44 -0500", "640 AO-TriggerSequenceLength": "1000", "470 AO-Name": "AnalogIO", "XLIGHT Disk slider-Label": "Disk Pos 40um", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "Binning": "1x1", "TE2000-ModelType": "1", "COM3-AnswerTimeout": "500.0000", "555 Shutter-SupportsTriggering": "0", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "TE2000-Port": "COM4", "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Description": "NI DAC", "COM4-AnswerTimeout": "500.0000", "COM1-DelayBetweenCharsMs": "0.0000", "FilterBlock-Label": "Confocal", "445 AO-Name": "AnalogIO", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-Timing-ReadoutTimeNs": "24672000.0000", "528 AO-Demo": "No", "520 AO-Sequenceable": "1", "555-Description": "DA device that is used as a shutter", "405-DA Device": "405 Shutter", "405 AO-TriggerSequenceLength": "1000", "640 AO-MinVolts": "0.0000", "640 Shutter-Block voltage": "false", "FrameIndex": 0, "PRIME-Port": "CMOS", "555 AO-MinVolts": "0.0000", "COM3-Description": "Serial port driver (boost:asio)", "528 AO-Description": "NI DAC", "555 AO-IOChannel": "Dev1/ao6", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "555 AO-TriggerInputLine": "/Dev1/PFI0", "PRIME-ActualInterval-ms": "390.5200", "LudlController-Port": "COM9", "445 Shutter-Demo": "No", "PRIME-Trigger-Expose Out-Mux": "1", "SliceIndex": 5, "PRIME-CCDTemperatureSetPoint": "-15.0000", "405 Shutter-MinVolts": "0.0000", "PRIME-ExposeOutMode": "First Row", "AxisPositions": null, "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Volts": "0.0000", "528-Name": "DA Shutter", "555-State": "0", "XLIGHT Dichroic-Label": "Di03-R635-t1", "445 AO-Sequenceable": "1", "405-Name": "DA Shutter", "405 AO-IOChannel": "Dev1/ao0", "Core-Camera": "PRIME", "Focus-Name": "Focus", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "405 Shutter-IOChannel": "Dev1/ao8", "640 AO-Demo": "No", "640 Shutter-SupportsTriggering": "0", "445 AO-Demo": "No", "555-DA Device": "555 Shutter", "470 Shutter-Description": "NI DAC", "528 AO-Volts": "0.0000", "COM1-DataBits": "8", "Vincent-D3-Address": "x", "520 AO-SupportsTriggering": "0", "405-Description": "DA device that is used as a shutter", "640 Shutter-MinVolts": "0.0000", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "405 AO-Name": "AnalogIO", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "640 AO-TriggerInputLine": "/Dev1/PFI0", "405 AO-TriggerInputLine": "/Dev1/PFI0", "640-Description": "DA device that is used as a shutter", "445 Shutter-TriggerSampleRate": "100000", "470 Shutter-TriggerSequenceLength": "1000", "COM1-StopBits": "1", "520 Shutter-SupportsTriggering": "0", "405-State": "0", "Core-Initialize": "1", "XLIGHT Emission Wheel-State": "3", "520 AO-Description": "NI DAC", "528 AO-TriggerInputLine": "/Dev1/PFI0", "445 Shutter-MaxVolts": "5.0000", "COM9-Handshaking": "Off", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "XLIGHT Spin motor-Label": "On (spinning)", "PRIME-PP 5 ENABLED": "No", "UUID": "ea34b530-bda5-4fc7-8004-cb8e9fc510cd", "PRIME-TriggerMode": "Internal Trigger", "COM1-Handshaking": "Off", "PRIME-Timing-PostTriggerDelayNs": "49344000.0000", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "Focus-Position": "17741.5000", "528 Shutter-Block voltage": "false", "Channel": "Il-C", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "555 Shutter-Demo": "No", "405 Shutter-Demo": "No", "528 AO-Name": "AnalogIO", "520-State": "0", "Core-Galvo": "", "405 Shutter-TriggerSampleRate": "100000", "Focus-FocusStepSize_(nm)": "100", "PRIME-PixelType": "16bit", "COM4-DataBits": "8", "470 AO-IOChannel": "Dev1/ao2", "520 AO-MinVolts": "0.0000", "COM9-Parity": "None", "PRIME-CircularBufferEnabled": "ON", "PRIME-PP 6 Reset": "No", "640 AO-Description": "NI DAC", "Source": "PRIME", "445 AO-MinVolts": "0.0000", "PIEZO-Name": "Stage", "445 AO-Description": "NI DAC", "PRIME-CircularBufferFrameCount": "46", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "520 AO-Volts": "0.0000", "528 Shutter-TriggerSampleRate": "100000", "XPositionUm": -1324.9, "OpticalPath-State": "4", "PRIME-PP 3 THRESHOLD": "80", "PRIME-PVCAM Version": "3.7.1", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Gain": "1-HDR", "640 Shutter-TriggerSequenceLength": "1000", "528 AO-SupportsTriggering": "0", "445 AO-TriggerInputLine": "/Dev1/PFI0", "OpticalPath-Name": "OpticalPath", "PRIME-PP 4 ENABLED": "Yes", "PRIME-PVCAM Adapter Version": "1.3.29", "COM3-Name": "COM3", "405 AO-MaxVolts": "5.0000", "520 Shutter-Block voltage": "false", "PRIME-Timing-ClearingTimeNs": "49344000.0000", "Core-Focus": "PIEZO", "528 AO-Block voltage": "false", "TE2000-Description": "Nikon TE2000 hub", "528 AO-MinVolts": "0.0000", "COM4-Parity": "None", "Summary": { "Slices": 11, "ChContrastMin": [ 0, 0, 0, 0, 0, 0 ], "PixelAspect": 1, "SlicesFirst": true, "Interval_ms": 0, "Time": "2019-12-19 09:59:21 -0500", "Prefix": "seq_13", "InitialPositionList": [ { "Label": "Pos0", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.21], "Focus": [17741.5], "XYStage": [ 44542.200000000004, 31636.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos1", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.22], "Focus": [17741.4], "XYStage": [ 43801.100000000006, 31637.9 ] }, "GridColumnIndex": 0 }, { "Label": "Pos2", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.5], "XYStage": [ 38561.3, 32040.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos3", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.185], "Focus": [17741.3], "XYStage": [ 37698.4, 32041.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos4", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.16], "Focus": [17741.5], "XYStage": [ 32375.4, 31758.9 ] }, "GridColumnIndex": 0 }, { "Label": "Pos5", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.17000000000002], "Focus": [17741.3], "XYStage": [ 31770.7, 31758.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos6", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.4], "XYStage": [ 26536.800000000003, 31068.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos7", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.205], "Focus": [17741.4], "XYStage": [ 25420.2, 31068.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos8", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.85], "Focus": [17741.3], "XYStage": [ 21059, 31508 ] }, "GridColumnIndex": 0 }, { "Label": "Pos9", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.82], "Focus": [17741.3], "XYStage": [ 19731.9, 31506.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos10", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.79], "Focus": [17741.4], "XYStage": [ 14528.5, 32007.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos11", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.83], "Focus": [17741.2], "XYStage": [ 13830.300000000001, 32009.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos12", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.79], "Focus": [17741.3], "XYStage": [ 8775.300000000001, 32017.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos13", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.815], "Focus": [17741.3], "XYStage": [ 8109.200000000001, 32008.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos14", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.805], "Focus": [17741.3], "XYStage": [ 3483.3, 31908.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos15", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.82], "Focus": [17741.2], "XYStage": [ 2686.8, 31903.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos16", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [211.48000000000002], "Focus": [17741.1], "XYStage": [ -1978.9, 31973.600000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos17", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [211.51500000000001], "Focus": [17741.1], "XYStage": [ -2957.8, 31982.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos18", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.195], "Focus": [17741.5], "XYStage": [ 47747.4, -576.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos19", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.23000000000002], "Focus": [17741.6], "XYStage": [ 47053.9, -576.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos20", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.205], "Focus": [17741.4], "XYStage": [ 41871.200000000004, -479.8 ] }, "GridColumnIndex": 0 }, { "Label": "Pos21", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.4], "XYStage": [ 40882.600000000006, -392 ] }, "GridColumnIndex": 0 }, { "Label": "Pos22", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.24], "Focus": [17741.4], "XYStage": [ 35905.6, -135.3 ] }, "GridColumnIndex": 0 }, { "Label": "Pos23", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.195], "Focus": [17741.5], "XYStage": [ 34990.9, -132.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos24", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.46], "Focus": [17741.4], "XYStage": [ 29678.100000000002, -958.1 ] }, "GridColumnIndex": 0 }, { "Label": "Pos25", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.44], "Focus": [17741.5], "XYStage": [ 28983.600000000002, -939.4000000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos26", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.475], "Focus": [17741.5], "XYStage": [ 23019.7, -122 ] }, "GridColumnIndex": 0 }, { "Label": "Pos27", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.44], "Focus": [17741.7], "XYStage": [ 22428, -116.80000000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos28", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.46], "Focus": [17741.7], "XYStage": [ 16364.900000000001, -181.10000000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos29", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.48000000000002], "Focus": [17741.5], "XYStage": [ 15712.1, -180 ] }, "GridColumnIndex": 0 }, { "Label": "Pos30", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.45000000000002], "Focus": [17741.7], "XYStage": [ 10346.1, 77.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos31", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.49], "Focus": [17741.4], "XYStage": [ 9755.5, 85.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos32", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.475], "Focus": [17741.5], "XYStage": [ 4775.5, 293.90000000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos33", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.445], "Focus": [17741.4], "XYStage": [ 3954.9, 293.90000000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos34", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.455], "Focus": [17741.4], "XYStage": [ -1324.9, 545.6 ] }, "GridColumnIndex": 0 }, { "Label": "Pos35", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.445], "Focus": [17741.3], "XYStage": [ -2097, 576.6 ] }, "GridColumnIndex": 0 } ], "z-step_um": 10, "GridRow": 0, "BitDepth": 16, "Depth": 2, "Channels": 6, "Source": "Micro-Manager", "ChColors": [ -16711681, -256, -65281, -1, -1, -16776961 ], "MetadataVersion": 10, "KeepShutterOpenChannels": true, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536, 65536 ], "CustomIntervals_ms": [], "Height": 1200, "MicroManagerVersion": "1.4.23 20180219", "GridColumn": 0, "UUID": "18808e7c-f2e6-47b3-90d8-1d779ce87dbd", "PixelType": "GRAY16", "Width": 1200, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\Yan\\20191218-YC113-S7S8-miseq", "Frames": 1, "Comment": "", "UserName": "Nikon", "PixelSize_um": 1.1, "ROI": [ 0, 0, 1200, 1200 ], "Date": "2019-12-19", "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC", "P-DAPI-high" ], "KeepShutterOpenSlices": true, "IJType": 1, "ComputerName": "ZadorConfocal2", "Positions": 36, "PositionIndex": 34, "CameraTimeout": 20000, "TimeFirst": false }, "640 AO-Name": "AnalogIO", "Vincent-D3-Command": "Close" }, "FrameKey-0-3-6": { "COM4-StopBits": "1", "528 AO-MaxVolts": "5.0000", "XLIGHT Excitation Wheel-State": "1", "470 Shutter-Name": "AnalogIO", "Core-Shutter": "640", "555 AO-Demo": "No", "XLIGHT Dichroic-State": "2", "XYStage-StepSize-Y": "0.1000", "ZPositionUm": 228.455, "XYStage-StepSize-X": "0.1000", "PRIME-FirmwareVersion": "20.28", "PRIME-ClearCycles": "2", "520-Name": "DA Shutter", "405 Shutter-Name": "AnalogIO", "PIEZO-StepSize": "0.0050", "PRIME-ClearMode": "Pre-Exposure", "470 AO-MinVolts": "0.0000", "405 AO-SupportsTriggering": "1", "CameraChannelIndex": 0, "XYStage-StartSpeed": "500.0000", "COM9-StopBits": "2", "405 AO-Description": "NI DAC", "COM4-DelayBetweenCharsMs": "0.0000", "445 AO-Block voltage": "false", "520 AO-TriggerInputLine": "/Dev1/PFI0", "555 AO-Description": "NI DAC", "520 AO-TriggerSampleRate": "100000", "COM4-Verbose": "1", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM4-Name": "COM4", "405 Shutter-Volts": "0.0000", "Vincent-D3-Port": "COM1", "PRIME-PP 4 MIN ADU AFFECTED": "200", "470 Shutter-MinVolts": "0.0000", "PRIME-PP 3 ENABLED": "Yes", "470 AO-Description": "NI DAC", "PRIME-Description": "PVCAM API device adapter", "520 Shutter-MaxVolts": "5.0000", "520 Shutter-Volts": "0.0000", "528 AO-Sequenceable": "1", "PRIME-Binning": "1x1", "555 Shutter-MaxVolts": "5.0000", "528 Shutter-TriggerSequenceLength": "1000", "405 Shutter-Block voltage": "false", "XLIGHT Spin motor-State": "1", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "Vincent-D3-Time to open (ms)": "35.0000", "Vincent-D3-Time to close (ms)": "35.0000", "445 AO-TriggerSampleRate": "100000", "640 Shutter-IOChannel": "Dev1/ao14", "555 AO-Sequenceable": "1", "520 AO-Name": "AnalogIO", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "470 AO-Block voltage": "false", "COM1-Verbose": "1", "COM9-AnswerTimeout": "10000.0000", "528 Shutter-Name": "AnalogIO", "PRIME-Trigger Timeout (secs)": "10", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "NextFrame": 0, "470 AO-TriggerSequenceLength": "1000", "PRIME-TransposeXY": "0", "445 AO-TriggerSequenceLength": "1000", "COM1-Name": "COM1", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "COM9-DataBits": "8", "COM1-AnswerTimeout": "500.0000", "Camera": "", "TE2000-Version": "V2.50-V2.50-V2.10", "528 Shutter-Volts": "0.0000", "XLIGHT Hub-Name": "XLIGHT Hub", "PIEZO-Description": "Ludl stage driver adapter", "PositionName": "Pos34", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSampleRate": "100000", "PRIME-FanSpeedSetpoint": "High", "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "405 AO-TriggerSampleRate": "100000", "470 Shutter-Block voltage": "false", "470-State": "0", "COM3-Parity": "None", "405 AO-Volts": "0.0000", "PRIME-TransposeMirrorY": "0", "PRIME-ChipName": "GS144BSI", "470 AO-Volts": "0.0000", "PRIME-TransposeMirrorX": "0", "445 Shutter-SupportsTriggering": "0", "470 Shutter-SupportsTriggering": "0", "XLIGHT Hub-Description": "XLIGHT hub", "PRIME-PreampOffLimit": "10000", "470 AO-Sequenceable": "1", "528 Shutter-MaxVolts": "5.0000", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "Core-XYStage": "XYStage", "470 AO-TriggerSampleRate": "100000", "PRIME-Timing-PreTriggerDelayNs": "24672000.0000", "528-DA Device": "528 Shutter", "Focus-Description": "Nikon TE2000 focus adapter", "FilterBlock-State": "5", "520 AO-Demo": "No", "COM3-DelayBetweenCharsMs": "0.0000", "470 Shutter-Volts": "0.0000", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "PRIME-PP 2 THRESHOLD": "125", "PRIME-PP 4 THRESHOLD": "75", "XYStage-Acceleration": "4.0000", "470 Shutter-Demo": "No", "PRIME-Name": "PMPCIECam00", "640-DA Device": "640 Shutter", "405 AO-Block voltage": "false", "ElapsedTime-ms": 1414997, "PRIME-Offset": "100", "445 AO-IOChannel": "Dev1/ao1", "640 AO-MaxVolts": "5.0000", "PositionIndex": 34, "PRIME-PP 1 ENABLED": "Yes", "COM1-BaudRate": "9600", "640-State": "1", "COM3-Verbose": "1", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "LudlController-TransmissionDelay": "0", "PRIME-FullWellCapacity": "62000", "PRIME-SMARTStreamingEnabled": "No", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "640 Shutter-Description": "NI DAC", "528-Description": "DA device that is used as a shutter", "COM4-Description": "Serial port driver (boost:asio)", "405 Shutter-Description": "NI DAC", "TE2000-Name": "TE2000", "528 Shutter-Description": "NI DAC", "COM9-Description": "Serial port driver (boost:asio)", "LudlController-Name": "LudlController", "445 Shutter-TriggerSequenceLength": "1000", "PRIME-SerialNumber": "A18B203008", "445 AO-Volts": "0.0000", "PRIME-X-dimension": "1200", "PRIME-ShutterCloseDelay": "0", "XLIGHT Disk slider-State": "2", "Position": "Default", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 AO-IOChannel": "Dev1/ao5", "520 AO-IOChannel": "Dev1/ao4", "528 AO-TriggerSequenceLength": "1000", "405 Shutter-MaxVolts": "5.0000", "470-DA Device": "470 Shutter", "PRIME-FTCapable": "No", "Core-ChannelGroup": "Channels", "555 AO-SupportsTriggering": "0", "PRIME-Y-dimension": "1200", "Frame": 0, "555 Shutter-Sequenceable": "1", "PRIME-Timing-ExposureTimeNs": "499998000.0000", "Core-ImageProcessor": "", "528-State": "0", "520 AO-Block voltage": "false", "XLIGHT Excitation Wheel-Label": "QUAD", "640 Shutter-Demo": "No", "COM3-StopBits": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "520 Shutter-IOChannel": "Dev1/ao11", "Width": 1200, "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-SupportsTriggering": "0", "640 AO-TriggerSampleRate": "100000", "520-Description": "DA device that is used as a shutter", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "405 Shutter-Sequenceable": "1", "405 Shutter-SupportsTriggering": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-TriggerSampleRate": "100000", "640 Shutter-MaxVolts": "5.0000", "555 AO-MaxVolts": "5.0000", "520 AO-TriggerSequenceLength": "1000", "COM3-BaudRate": "9600", "COM9-BaudRate": "9600", "Vincent-D3-Channel #": "Ch. #1", "PRIME-PMode": "Normal", "OpticalPath-Label": "Left(100)", "BitDepth": 16, "555 AO-TriggerSequenceLength": "1000", "445 Shutter-Description": "NI DAC", "405 AO-Sequenceable": "1", "PRIME-AcquisitionMethod": "Callbacks", "555 AO-Name": "AnalogIO", "PRIME-ShutterMode": "Pre-Exposure", "COM3-Handshaking": "Off", "445 AO-SupportsTriggering": "0", "ROI": "0-0-1200-1200", "COM9-Verbose": "1", "PRIME-Color": "OFF", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "PRIME-ShutterOpenDelay": "0", "COM1-Parity": "None", "Slice": 6, "SlicePosition": 10, "COM9-DelayBetweenCharsMs": "0.0000", "555-Name": "DA Shutter", "PIEZO-LudlSingleAxisName": "B", "XLIGHT Emission Wheel-Label": "725/40 (C)", "640 Shutter-Name": "AnalogIO", "640 AO-Sequenceable": "1", "520 Shutter-Demo": "No", "528 Shutter-Demo": "No", "470-Name": "DA Shutter", "520-DA Device": "520 Shutter", "XYStage-Description": "Ludl XY stage driver adapter", "445 Shutter-MinVolts": "0.0000", "528 AO-TriggerSampleRate": "100000", "405 AO-MinVolts": "0.0000", "405 Shutter-TriggerSequenceLength": "1000", "Height": 1200, "555 AO-Block voltage": "false", "640 Shutter-Volts": "5.0000", "PixelType": "GRAY16", "445 Shutter-Block voltage": "false", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "520 Shutter-TriggerSequenceLength": "1000", "COM9-Name": "COM9", "Core-SLM": "", "555 AO-Volts": "0.0000", "555 Shutter-Block voltage": "false", "XYStage-Speed": "1800.0000", "520 AO-MaxVolts": "5.0000", "528 Shutter-SupportsTriggering": "0", "COM1-Description": "Serial port driver (boost:asio)", "PRIME-MetadataEnabled": "No", "520 Shutter-MinVolts": "0.0000", "470 Shutter-Sequenceable": "1", "PRIME-PreampDelay": "0", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-MinVolts": "0.0000", "ChannelIndex": 3, "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Core-AutoShutter": "1", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "Exposure-ms": 520, "PRIME-PP 2 ENABLED": "Yes", "640 Shutter-TriggerSampleRate": "100000", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "470 Shutter-IOChannel": "Dev1/ao10", "405 AO-Demo": "No", "PixelSizeUm": 1.1, "470 AO-Demo": "No", "470 Shutter-MaxVolts": "5.0000", "Vincent-D3-Name": "Vincent-D3", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FileName": "img_000000000_Il-C_006.tif", "LudlController-Reset": "Operate", "PRIME-Exposure": "520.00", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "445-Name": "DA Shutter", "555 Shutter-Name": "AnalogIO", "520 Shutter-TriggerSampleRate": "100000", "445 Shutter-IOChannel": "Dev1/ao9", "PRIME-TransposeCorrection": "0", "XYStage-StepSize": "0.1000", "445-Description": "DA device that is used as a shutter", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 AO-IOChannel": "Dev1/ao7", "520 Shutter-Name": "AnalogIO", "COM3-DataBits": "8", "640 AO-Volts": "4.5000", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "555 Shutter-IOChannel": "Dev1/ao13", "WaitInterval": null, "520 Shutter-Sequenceable": "1", "YPositionUm": 545.6, "445-State": "0", "445-DA Device": "445 Shutter", "PRIME-CircularBufferAutoSize": "ON", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "555 AO-TriggerSampleRate": "100000", "445 Shutter-Volts": "0.0000", "XLIGHT Hub-Port": "COM3", "Core-AutoFocus": "", "COM4-Handshaking": "Off", "528 Shutter-MinVolts": "0.0000", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "445 Shutter-Name": "AnalogIO", "528 Shutter-Sequenceable": "1", "XYStage-TransposeMirrorX": "0", "XYStage-TransposeMirrorY": "0", "PRIME-ReadoutRate": "100MHz 16bit", "XYStage-Name": "Stage", "640 AO-SupportsTriggering": "0", "640-Name": "DA Shutter", "640 Shutter-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "640 AO-Block voltage": "false", "445 Shutter-Sequenceable": "1", "470 AO-MaxVolts": "5.0000", "COM4-BaudRate": "9600", "470-Description": "DA device that is used as a shutter", "520 Shutter-Description": "NI DAC", "PRIME-CCDTemperature": "-14.8500", "Time": "2019-12-19 09:59:45 -0500", "640 AO-TriggerSequenceLength": "1000", "470 AO-Name": "AnalogIO", "XLIGHT Disk slider-Label": "Disk Pos 40um", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "Binning": "1x1", "TE2000-ModelType": "1", "COM3-AnswerTimeout": "500.0000", "555 Shutter-SupportsTriggering": "0", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "TE2000-Port": "COM4", "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Description": "NI DAC", "COM4-AnswerTimeout": "500.0000", "COM1-DelayBetweenCharsMs": "0.0000", "FilterBlock-Label": "Confocal", "445 AO-Name": "AnalogIO", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-Timing-ReadoutTimeNs": "24672000.0000", "528 AO-Demo": "No", "520 AO-Sequenceable": "1", "555-Description": "DA device that is used as a shutter", "405-DA Device": "405 Shutter", "405 AO-TriggerSequenceLength": "1000", "640 AO-MinVolts": "0.0000", "640 Shutter-Block voltage": "false", "FrameIndex": 0, "PRIME-Port": "CMOS", "555 AO-MinVolts": "0.0000", "COM3-Description": "Serial port driver (boost:asio)", "528 AO-Description": "NI DAC", "555 AO-IOChannel": "Dev1/ao6", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "555 AO-TriggerInputLine": "/Dev1/PFI0", "PRIME-ActualInterval-ms": "390.5200", "LudlController-Port": "COM9", "445 Shutter-Demo": "No", "PRIME-Trigger-Expose Out-Mux": "1", "SliceIndex": 6, "PRIME-CCDTemperatureSetPoint": "-15.0000", "405 Shutter-MinVolts": "0.0000", "PRIME-ExposeOutMode": "First Row", "AxisPositions": null, "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Volts": "0.0000", "528-Name": "DA Shutter", "555-State": "0", "XLIGHT Dichroic-Label": "Di03-R635-t1", "445 AO-Sequenceable": "1", "405-Name": "DA Shutter", "405 AO-IOChannel": "Dev1/ao0", "Core-Camera": "PRIME", "Focus-Name": "Focus", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "405 Shutter-IOChannel": "Dev1/ao8", "640 AO-Demo": "No", "640 Shutter-SupportsTriggering": "0", "445 AO-Demo": "No", "555-DA Device": "555 Shutter", "470 Shutter-Description": "NI DAC", "528 AO-Volts": "0.0000", "COM1-DataBits": "8", "Vincent-D3-Address": "x", "520 AO-SupportsTriggering": "0", "405-Description": "DA device that is used as a shutter", "640 Shutter-MinVolts": "0.0000", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "405 AO-Name": "AnalogIO", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "640 AO-TriggerInputLine": "/Dev1/PFI0", "405 AO-TriggerInputLine": "/Dev1/PFI0", "640-Description": "DA device that is used as a shutter", "445 Shutter-TriggerSampleRate": "100000", "470 Shutter-TriggerSequenceLength": "1000", "COM1-StopBits": "1", "520 Shutter-SupportsTriggering": "0", "405-State": "0", "Core-Initialize": "1", "XLIGHT Emission Wheel-State": "3", "520 AO-Description": "NI DAC", "528 AO-TriggerInputLine": "/Dev1/PFI0", "445 Shutter-MaxVolts": "5.0000", "COM9-Handshaking": "Off", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "XLIGHT Spin motor-Label": "On (spinning)", "PRIME-PP 5 ENABLED": "No", "UUID": "c5fec56d-4c29-4f2d-8d64-5f9bc4c62741", "PRIME-TriggerMode": "Internal Trigger", "COM1-Handshaking": "Off", "PRIME-Timing-PostTriggerDelayNs": "49344000.0000", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "Focus-Position": "17741.5000", "528 Shutter-Block voltage": "false", "Channel": "Il-C", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "555 Shutter-Demo": "No", "405 Shutter-Demo": "No", "528 AO-Name": "AnalogIO", "520-State": "0", "Core-Galvo": "", "405 Shutter-TriggerSampleRate": "100000", "Focus-FocusStepSize_(nm)": "100", "PRIME-PixelType": "16bit", "COM4-DataBits": "8", "470 AO-IOChannel": "Dev1/ao2", "520 AO-MinVolts": "0.0000", "COM9-Parity": "None", "PRIME-CircularBufferEnabled": "ON", "PRIME-PP 6 Reset": "No", "640 AO-Description": "NI DAC", "Source": "PRIME", "445 AO-MinVolts": "0.0000", "PIEZO-Name": "Stage", "445 AO-Description": "NI DAC", "PRIME-CircularBufferFrameCount": "46", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "520 AO-Volts": "0.0000", "528 Shutter-TriggerSampleRate": "100000", "XPositionUm": -1324.9, "OpticalPath-State": "4", "PRIME-PP 3 THRESHOLD": "80", "PRIME-PVCAM Version": "3.7.1", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Gain": "1-HDR", "640 Shutter-TriggerSequenceLength": "1000", "528 AO-SupportsTriggering": "0", "445 AO-TriggerInputLine": "/Dev1/PFI0", "OpticalPath-Name": "OpticalPath", "PRIME-PP 4 ENABLED": "Yes", "PRIME-PVCAM Adapter Version": "1.3.29", "COM3-Name": "COM3", "405 AO-MaxVolts": "5.0000", "520 Shutter-Block voltage": "false", "PRIME-Timing-ClearingTimeNs": "49344000.0000", "Core-Focus": "PIEZO", "528 AO-Block voltage": "false", "TE2000-Description": "Nikon TE2000 hub", "528 AO-MinVolts": "0.0000", "COM4-Parity": "None", "Summary": { "Slices": 11, "ChContrastMin": [ 0, 0, 0, 0, 0, 0 ], "PixelAspect": 1, "SlicesFirst": true, "Interval_ms": 0, "Time": "2019-12-19 09:59:21 -0500", "Prefix": "seq_13", "InitialPositionList": [ { "Label": "Pos0", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.21], "Focus": [17741.5], "XYStage": [ 44542.200000000004, 31636.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos1", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.22], "Focus": [17741.4], "XYStage": [ 43801.100000000006, 31637.9 ] }, "GridColumnIndex": 0 }, { "Label": "Pos2", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.5], "XYStage": [ 38561.3, 32040.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos3", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.185], "Focus": [17741.3], "XYStage": [ 37698.4, 32041.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos4", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.16], "Focus": [17741.5], "XYStage": [ 32375.4, 31758.9 ] }, "GridColumnIndex": 0 }, { "Label": "Pos5", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.17000000000002], "Focus": [17741.3], "XYStage": [ 31770.7, 31758.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos6", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.4], "XYStage": [ 26536.800000000003, 31068.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos7", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.205], "Focus": [17741.4], "XYStage": [ 25420.2, 31068.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos8", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.85], "Focus": [17741.3], "XYStage": [ 21059, 31508 ] }, "GridColumnIndex": 0 }, { "Label": "Pos9", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.82], "Focus": [17741.3], "XYStage": [ 19731.9, 31506.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos10", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.79], "Focus": [17741.4], "XYStage": [ 14528.5, 32007.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos11", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.83], "Focus": [17741.2], "XYStage": [ 13830.300000000001, 32009.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos12", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.79], "Focus": [17741.3], "XYStage": [ 8775.300000000001, 32017.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos13", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.815], "Focus": [17741.3], "XYStage": [ 8109.200000000001, 32008.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos14", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.805], "Focus": [17741.3], "XYStage": [ 3483.3, 31908.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos15", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.82], "Focus": [17741.2], "XYStage": [ 2686.8, 31903.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos16", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [211.48000000000002], "Focus": [17741.1], "XYStage": [ -1978.9, 31973.600000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos17", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [211.51500000000001], "Focus": [17741.1], "XYStage": [ -2957.8, 31982.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos18", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.195], "Focus": [17741.5], "XYStage": [ 47747.4, -576.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos19", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.23000000000002], "Focus": [17741.6], "XYStage": [ 47053.9, -576.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos20", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.205], "Focus": [17741.4], "XYStage": [ 41871.200000000004, -479.8 ] }, "GridColumnIndex": 0 }, { "Label": "Pos21", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.4], "XYStage": [ 40882.600000000006, -392 ] }, "GridColumnIndex": 0 }, { "Label": "Pos22", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.24], "Focus": [17741.4], "XYStage": [ 35905.6, -135.3 ] }, "GridColumnIndex": 0 }, { "Label": "Pos23", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.195], "Focus": [17741.5], "XYStage": [ 34990.9, -132.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos24", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.46], "Focus": [17741.4], "XYStage": [ 29678.100000000002, -958.1 ] }, "GridColumnIndex": 0 }, { "Label": "Pos25", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.44], "Focus": [17741.5], "XYStage": [ 28983.600000000002, -939.4000000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos26", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.475], "Focus": [17741.5], "XYStage": [ 23019.7, -122 ] }, "GridColumnIndex": 0 }, { "Label": "Pos27", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.44], "Focus": [17741.7], "XYStage": [ 22428, -116.80000000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos28", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.46], "Focus": [17741.7], "XYStage": [ 16364.900000000001, -181.10000000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos29", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.48000000000002], "Focus": [17741.5], "XYStage": [ 15712.1, -180 ] }, "GridColumnIndex": 0 }, { "Label": "Pos30", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.45000000000002], "Focus": [17741.7], "XYStage": [ 10346.1, 77.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos31", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.49], "Focus": [17741.4], "XYStage": [ 9755.5, 85.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos32", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.475], "Focus": [17741.5], "XYStage": [ 4775.5, 293.90000000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos33", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.445], "Focus": [17741.4], "XYStage": [ 3954.9, 293.90000000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos34", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.455], "Focus": [17741.4], "XYStage": [ -1324.9, 545.6 ] }, "GridColumnIndex": 0 }, { "Label": "Pos35", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.445], "Focus": [17741.3], "XYStage": [ -2097, 576.6 ] }, "GridColumnIndex": 0 } ], "z-step_um": 10, "GridRow": 0, "BitDepth": 16, "Depth": 2, "Channels": 6, "Source": "Micro-Manager", "ChColors": [ -16711681, -256, -65281, -1, -1, -16776961 ], "MetadataVersion": 10, "KeepShutterOpenChannels": true, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536, 65536 ], "CustomIntervals_ms": [], "Height": 1200, "MicroManagerVersion": "1.4.23 20180219", "GridColumn": 0, "UUID": "18808e7c-f2e6-47b3-90d8-1d779ce87dbd", "PixelType": "GRAY16", "Width": 1200, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\Yan\\20191218-YC113-S7S8-miseq", "Frames": 1, "Comment": "", "UserName": "Nikon", "PixelSize_um": 1.1, "ROI": [ 0, 0, 1200, 1200 ], "Date": "2019-12-19", "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC", "P-DAPI-high" ], "KeepShutterOpenSlices": true, "IJType": 1, "ComputerName": "ZadorConfocal2", "Positions": 36, "PositionIndex": 34, "CameraTimeout": 20000, "TimeFirst": false }, "640 AO-Name": "AnalogIO", "Vincent-D3-Command": "Close" }, "FrameKey-0-3-7": { "COM4-StopBits": "1", "528 AO-MaxVolts": "5.0000", "XLIGHT Excitation Wheel-State": "1", "470 Shutter-Name": "AnalogIO", "Core-Shutter": "640", "555 AO-Demo": "No", "XLIGHT Dichroic-State": "2", "XYStage-StepSize-Y": "0.1000", "ZPositionUm": 238.455, "XYStage-StepSize-X": "0.1000", "PRIME-FirmwareVersion": "20.28", "PRIME-ClearCycles": "2", "520-Name": "DA Shutter", "405 Shutter-Name": "AnalogIO", "PIEZO-StepSize": "0.0050", "PRIME-ClearMode": "Pre-Exposure", "470 AO-MinVolts": "0.0000", "405 AO-SupportsTriggering": "1", "CameraChannelIndex": 0, "XYStage-StartSpeed": "500.0000", "COM9-StopBits": "2", "405 AO-Description": "NI DAC", "COM4-DelayBetweenCharsMs": "0.0000", "445 AO-Block voltage": "false", "520 AO-TriggerInputLine": "/Dev1/PFI0", "555 AO-Description": "NI DAC", "520 AO-TriggerSampleRate": "100000", "COM4-Verbose": "1", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM4-Name": "COM4", "405 Shutter-Volts": "0.0000", "Vincent-D3-Port": "COM1", "PRIME-PP 4 MIN ADU AFFECTED": "200", "470 Shutter-MinVolts": "0.0000", "PRIME-PP 3 ENABLED": "Yes", "470 AO-Description": "NI DAC", "PRIME-Description": "PVCAM API device adapter", "520 Shutter-MaxVolts": "5.0000", "520 Shutter-Volts": "0.0000", "528 AO-Sequenceable": "1", "PRIME-Binning": "1x1", "555 Shutter-MaxVolts": "5.0000", "528 Shutter-TriggerSequenceLength": "1000", "405 Shutter-Block voltage": "false", "XLIGHT Spin motor-State": "1", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "Vincent-D3-Time to open (ms)": "35.0000", "Vincent-D3-Time to close (ms)": "35.0000", "445 AO-TriggerSampleRate": "100000", "640 Shutter-IOChannel": "Dev1/ao14", "555 AO-Sequenceable": "1", "520 AO-Name": "AnalogIO", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "470 AO-Block voltage": "false", "COM1-Verbose": "1", "COM9-AnswerTimeout": "10000.0000", "528 Shutter-Name": "AnalogIO", "PRIME-Trigger Timeout (secs)": "10", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "NextFrame": 0, "470 AO-TriggerSequenceLength": "1000", "PRIME-TransposeXY": "0", "445 AO-TriggerSequenceLength": "1000", "COM1-Name": "COM1", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "COM9-DataBits": "8", "COM1-AnswerTimeout": "500.0000", "Camera": "", "TE2000-Version": "V2.50-V2.50-V2.10", "528 Shutter-Volts": "0.0000", "XLIGHT Hub-Name": "XLIGHT Hub", "PIEZO-Description": "Ludl stage driver adapter", "PositionName": "Pos34", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSampleRate": "100000", "PRIME-FanSpeedSetpoint": "High", "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "405 AO-TriggerSampleRate": "100000", "470 Shutter-Block voltage": "false", "470-State": "0", "COM3-Parity": "None", "405 AO-Volts": "0.0000", "PRIME-TransposeMirrorY": "0", "PRIME-ChipName": "GS144BSI", "470 AO-Volts": "0.0000", "PRIME-TransposeMirrorX": "0", "445 Shutter-SupportsTriggering": "0", "470 Shutter-SupportsTriggering": "0", "XLIGHT Hub-Description": "XLIGHT hub", "PRIME-PreampOffLimit": "10000", "470 AO-Sequenceable": "1", "528 Shutter-MaxVolts": "5.0000", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "Core-XYStage": "XYStage", "470 AO-TriggerSampleRate": "100000", "PRIME-Timing-PreTriggerDelayNs": "24672000.0000", "528-DA Device": "528 Shutter", "Focus-Description": "Nikon TE2000 focus adapter", "FilterBlock-State": "5", "520 AO-Demo": "No", "COM3-DelayBetweenCharsMs": "0.0000", "470 Shutter-Volts": "0.0000", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "PRIME-PP 2 THRESHOLD": "125", "PRIME-PP 4 THRESHOLD": "75", "XYStage-Acceleration": "4.0000", "470 Shutter-Demo": "No", "PRIME-Name": "PMPCIECam00", "640-DA Device": "640 Shutter", "405 AO-Block voltage": "false", "ElapsedTime-ms": 1415759, "PRIME-Offset": "100", "445 AO-IOChannel": "Dev1/ao1", "640 AO-MaxVolts": "5.0000", "PositionIndex": 34, "PRIME-PP 1 ENABLED": "Yes", "COM1-BaudRate": "9600", "640-State": "1", "COM3-Verbose": "1", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "LudlController-TransmissionDelay": "0", "PRIME-FullWellCapacity": "62000", "PRIME-SMARTStreamingEnabled": "No", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "640 Shutter-Description": "NI DAC", "528-Description": "DA device that is used as a shutter", "COM4-Description": "Serial port driver (boost:asio)", "405 Shutter-Description": "NI DAC", "TE2000-Name": "TE2000", "528 Shutter-Description": "NI DAC", "COM9-Description": "Serial port driver (boost:asio)", "LudlController-Name": "LudlController", "445 Shutter-TriggerSequenceLength": "1000", "PRIME-SerialNumber": "A18B203008", "445 AO-Volts": "0.0000", "PRIME-X-dimension": "1200", "PRIME-ShutterCloseDelay": "0", "XLIGHT Disk slider-State": "2", "Position": "Default", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 AO-IOChannel": "Dev1/ao5", "520 AO-IOChannel": "Dev1/ao4", "528 AO-TriggerSequenceLength": "1000", "405 Shutter-MaxVolts": "5.0000", "470-DA Device": "470 Shutter", "PRIME-FTCapable": "No", "Core-ChannelGroup": "Channels", "555 AO-SupportsTriggering": "0", "PRIME-Y-dimension": "1200", "Frame": 0, "555 Shutter-Sequenceable": "1", "PRIME-Timing-ExposureTimeNs": "499998000.0000", "Core-ImageProcessor": "", "528-State": "0", "520 AO-Block voltage": "false", "XLIGHT Excitation Wheel-Label": "QUAD", "640 Shutter-Demo": "No", "COM3-StopBits": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "520 Shutter-IOChannel": "Dev1/ao11", "Width": 1200, "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-SupportsTriggering": "0", "640 AO-TriggerSampleRate": "100000", "520-Description": "DA device that is used as a shutter", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "405 Shutter-Sequenceable": "1", "405 Shutter-SupportsTriggering": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-TriggerSampleRate": "100000", "640 Shutter-MaxVolts": "5.0000", "555 AO-MaxVolts": "5.0000", "520 AO-TriggerSequenceLength": "1000", "COM3-BaudRate": "9600", "COM9-BaudRate": "9600", "Vincent-D3-Channel #": "Ch. #1", "PRIME-PMode": "Normal", "OpticalPath-Label": "Left(100)", "BitDepth": 16, "555 AO-TriggerSequenceLength": "1000", "445 Shutter-Description": "NI DAC", "405 AO-Sequenceable": "1", "PRIME-AcquisitionMethod": "Callbacks", "555 AO-Name": "AnalogIO", "PRIME-ShutterMode": "Pre-Exposure", "COM3-Handshaking": "Off", "445 AO-SupportsTriggering": "0", "ROI": "0-0-1200-1200", "COM9-Verbose": "1", "PRIME-Color": "OFF", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "PRIME-ShutterOpenDelay": "0", "COM1-Parity": "None", "Slice": 7, "SlicePosition": 20, "COM9-DelayBetweenCharsMs": "0.0000", "555-Name": "DA Shutter", "PIEZO-LudlSingleAxisName": "B", "XLIGHT Emission Wheel-Label": "725/40 (C)", "640 Shutter-Name": "AnalogIO", "640 AO-Sequenceable": "1", "520 Shutter-Demo": "No", "528 Shutter-Demo": "No", "470-Name": "DA Shutter", "520-DA Device": "520 Shutter", "XYStage-Description": "Ludl XY stage driver adapter", "445 Shutter-MinVolts": "0.0000", "528 AO-TriggerSampleRate": "100000", "405 AO-MinVolts": "0.0000", "405 Shutter-TriggerSequenceLength": "1000", "Height": 1200, "555 AO-Block voltage": "false", "640 Shutter-Volts": "5.0000", "PixelType": "GRAY16", "445 Shutter-Block voltage": "false", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "520 Shutter-TriggerSequenceLength": "1000", "COM9-Name": "COM9", "Core-SLM": "", "555 AO-Volts": "0.0000", "555 Shutter-Block voltage": "false", "XYStage-Speed": "1800.0000", "520 AO-MaxVolts": "5.0000", "528 Shutter-SupportsTriggering": "0", "COM1-Description": "Serial port driver (boost:asio)", "PRIME-MetadataEnabled": "No", "520 Shutter-MinVolts": "0.0000", "470 Shutter-Sequenceable": "1", "PRIME-PreampDelay": "0", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-MinVolts": "0.0000", "ChannelIndex": 3, "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Core-AutoShutter": "1", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "Exposure-ms": 520, "PRIME-PP 2 ENABLED": "Yes", "640 Shutter-TriggerSampleRate": "100000", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "470 Shutter-IOChannel": "Dev1/ao10", "405 AO-Demo": "No", "PixelSizeUm": 1.1, "470 AO-Demo": "No", "470 Shutter-MaxVolts": "5.0000", "Vincent-D3-Name": "Vincent-D3", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FileName": "img_000000000_Il-C_007.tif", "LudlController-Reset": "Operate", "PRIME-Exposure": "520.00", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "445-Name": "DA Shutter", "555 Shutter-Name": "AnalogIO", "520 Shutter-TriggerSampleRate": "100000", "445 Shutter-IOChannel": "Dev1/ao9", "PRIME-TransposeCorrection": "0", "XYStage-StepSize": "0.1000", "445-Description": "DA device that is used as a shutter", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 AO-IOChannel": "Dev1/ao7", "520 Shutter-Name": "AnalogIO", "COM3-DataBits": "8", "640 AO-Volts": "4.5000", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "555 Shutter-IOChannel": "Dev1/ao13", "WaitInterval": null, "520 Shutter-Sequenceable": "1", "YPositionUm": 545.6, "445-State": "0", "445-DA Device": "445 Shutter", "PRIME-CircularBufferAutoSize": "ON", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "555 AO-TriggerSampleRate": "100000", "445 Shutter-Volts": "0.0000", "XLIGHT Hub-Port": "COM3", "Core-AutoFocus": "", "COM4-Handshaking": "Off", "528 Shutter-MinVolts": "0.0000", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "445 Shutter-Name": "AnalogIO", "528 Shutter-Sequenceable": "1", "XYStage-TransposeMirrorX": "0", "XYStage-TransposeMirrorY": "0", "PRIME-ReadoutRate": "100MHz 16bit", "XYStage-Name": "Stage", "640 AO-SupportsTriggering": "0", "640-Name": "DA Shutter", "640 Shutter-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "640 AO-Block voltage": "false", "445 Shutter-Sequenceable": "1", "470 AO-MaxVolts": "5.0000", "COM4-BaudRate": "9600", "470-Description": "DA device that is used as a shutter", "520 Shutter-Description": "NI DAC", "PRIME-CCDTemperature": "-14.8500", "Time": "2019-12-19 09:59:46 -0500", "640 AO-TriggerSequenceLength": "1000", "470 AO-Name": "AnalogIO", "XLIGHT Disk slider-Label": "Disk Pos 40um", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "Binning": "1x1", "TE2000-ModelType": "1", "COM3-AnswerTimeout": "500.0000", "555 Shutter-SupportsTriggering": "0", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "TE2000-Port": "COM4", "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Description": "NI DAC", "COM4-AnswerTimeout": "500.0000", "COM1-DelayBetweenCharsMs": "0.0000", "FilterBlock-Label": "Confocal", "445 AO-Name": "AnalogIO", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-Timing-ReadoutTimeNs": "24672000.0000", "528 AO-Demo": "No", "520 AO-Sequenceable": "1", "555-Description": "DA device that is used as a shutter", "405-DA Device": "405 Shutter", "405 AO-TriggerSequenceLength": "1000", "640 AO-MinVolts": "0.0000", "640 Shutter-Block voltage": "false", "FrameIndex": 0, "PRIME-Port": "CMOS", "555 AO-MinVolts": "0.0000", "COM3-Description": "Serial port driver (boost:asio)", "528 AO-Description": "NI DAC", "555 AO-IOChannel": "Dev1/ao6", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "555 AO-TriggerInputLine": "/Dev1/PFI0", "PRIME-ActualInterval-ms": "390.5200", "LudlController-Port": "COM9", "445 Shutter-Demo": "No", "PRIME-Trigger-Expose Out-Mux": "1", "SliceIndex": 7, "PRIME-CCDTemperatureSetPoint": "-15.0000", "405 Shutter-MinVolts": "0.0000", "PRIME-ExposeOutMode": "First Row", "AxisPositions": null, "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Volts": "0.0000", "528-Name": "DA Shutter", "555-State": "0", "XLIGHT Dichroic-Label": "Di03-R635-t1", "445 AO-Sequenceable": "1", "405-Name": "DA Shutter", "405 AO-IOChannel": "Dev1/ao0", "Core-Camera": "PRIME", "Focus-Name": "Focus", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "405 Shutter-IOChannel": "Dev1/ao8", "640 AO-Demo": "No", "640 Shutter-SupportsTriggering": "0", "445 AO-Demo": "No", "555-DA Device": "555 Shutter", "470 Shutter-Description": "NI DAC", "528 AO-Volts": "0.0000", "COM1-DataBits": "8", "Vincent-D3-Address": "x", "520 AO-SupportsTriggering": "0", "405-Description": "DA device that is used as a shutter", "640 Shutter-MinVolts": "0.0000", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "405 AO-Name": "AnalogIO", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "640 AO-TriggerInputLine": "/Dev1/PFI0", "405 AO-TriggerInputLine": "/Dev1/PFI0", "640-Description": "DA device that is used as a shutter", "445 Shutter-TriggerSampleRate": "100000", "470 Shutter-TriggerSequenceLength": "1000", "COM1-StopBits": "1", "520 Shutter-SupportsTriggering": "0", "405-State": "0", "Core-Initialize": "1", "XLIGHT Emission Wheel-State": "3", "520 AO-Description": "NI DAC", "528 AO-TriggerInputLine": "/Dev1/PFI0", "445 Shutter-MaxVolts": "5.0000", "COM9-Handshaking": "Off", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "XLIGHT Spin motor-Label": "On (spinning)", "PRIME-PP 5 ENABLED": "No", "UUID": "70618230-4db7-4d73-9286-e49686b27498", "PRIME-TriggerMode": "Internal Trigger", "COM1-Handshaking": "Off", "PRIME-Timing-PostTriggerDelayNs": "49344000.0000", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "Focus-Position": "17741.5000", "528 Shutter-Block voltage": "false", "Channel": "Il-C", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "555 Shutter-Demo": "No", "405 Shutter-Demo": "No", "528 AO-Name": "AnalogIO", "520-State": "0", "Core-Galvo": "", "405 Shutter-TriggerSampleRate": "100000", "Focus-FocusStepSize_(nm)": "100", "PRIME-PixelType": "16bit", "COM4-DataBits": "8", "470 AO-IOChannel": "Dev1/ao2", "520 AO-MinVolts": "0.0000", "COM9-Parity": "None", "PRIME-CircularBufferEnabled": "ON", "PRIME-PP 6 Reset": "No", "640 AO-Description": "NI DAC", "Source": "PRIME", "445 AO-MinVolts": "0.0000", "PIEZO-Name": "Stage", "445 AO-Description": "NI DAC", "PRIME-CircularBufferFrameCount": "46", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "520 AO-Volts": "0.0000", "528 Shutter-TriggerSampleRate": "100000", "XPositionUm": -1324.9, "OpticalPath-State": "4", "PRIME-PP 3 THRESHOLD": "80", "PRIME-PVCAM Version": "3.7.1", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Gain": "1-HDR", "640 Shutter-TriggerSequenceLength": "1000", "528 AO-SupportsTriggering": "0", "445 AO-TriggerInputLine": "/Dev1/PFI0", "OpticalPath-Name": "OpticalPath", "PRIME-PP 4 ENABLED": "Yes", "PRIME-PVCAM Adapter Version": "1.3.29", "COM3-Name": "COM3", "405 AO-MaxVolts": "5.0000", "520 Shutter-Block voltage": "false", "PRIME-Timing-ClearingTimeNs": "49344000.0000", "Core-Focus": "PIEZO", "528 AO-Block voltage": "false", "TE2000-Description": "Nikon TE2000 hub", "528 AO-MinVolts": "0.0000", "COM4-Parity": "None", "Summary": { "Slices": 11, "ChContrastMin": [ 0, 0, 0, 0, 0, 0 ], "PixelAspect": 1, "SlicesFirst": true, "Interval_ms": 0, "Time": "2019-12-19 09:59:21 -0500", "Prefix": "seq_13", "InitialPositionList": [ { "Label": "Pos0", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.21], "Focus": [17741.5], "XYStage": [ 44542.200000000004, 31636.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos1", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.22], "Focus": [17741.4], "XYStage": [ 43801.100000000006, 31637.9 ] }, "GridColumnIndex": 0 }, { "Label": "Pos2", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.5], "XYStage": [ 38561.3, 32040.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos3", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.185], "Focus": [17741.3], "XYStage": [ 37698.4, 32041.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos4", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.16], "Focus": [17741.5], "XYStage": [ 32375.4, 31758.9 ] }, "GridColumnIndex": 0 }, { "Label": "Pos5", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.17000000000002], "Focus": [17741.3], "XYStage": [ 31770.7, 31758.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos6", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.4], "XYStage": [ 26536.800000000003, 31068.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos7", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.205], "Focus": [17741.4], "XYStage": [ 25420.2, 31068.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos8", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.85], "Focus": [17741.3], "XYStage": [ 21059, 31508 ] }, "GridColumnIndex": 0 }, { "Label": "Pos9", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.82], "Focus": [17741.3], "XYStage": [ 19731.9, 31506.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos10", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.79], "Focus": [17741.4], "XYStage": [ 14528.5, 32007.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos11", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.83], "Focus": [17741.2], "XYStage": [ 13830.300000000001, 32009.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos12", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.79], "Focus": [17741.3], "XYStage": [ 8775.300000000001, 32017.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos13", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.815], "Focus": [17741.3], "XYStage": [ 8109.200000000001, 32008.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos14", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.805], "Focus": [17741.3], "XYStage": [ 3483.3, 31908.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos15", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.82], "Focus": [17741.2], "XYStage": [ 2686.8, 31903.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos16", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [211.48000000000002], "Focus": [17741.1], "XYStage": [ -1978.9, 31973.600000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos17", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [211.51500000000001], "Focus": [17741.1], "XYStage": [ -2957.8, 31982.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos18", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.195], "Focus": [17741.5], "XYStage": [ 47747.4, -576.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos19", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.23000000000002], "Focus": [17741.6], "XYStage": [ 47053.9, -576.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos20", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.205], "Focus": [17741.4], "XYStage": [ 41871.200000000004, -479.8 ] }, "GridColumnIndex": 0 }, { "Label": "Pos21", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.4], "XYStage": [ 40882.600000000006, -392 ] }, "GridColumnIndex": 0 }, { "Label": "Pos22", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.24], "Focus": [17741.4], "XYStage": [ 35905.6, -135.3 ] }, "GridColumnIndex": 0 }, { "Label": "Pos23", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.195], "Focus": [17741.5], "XYStage": [ 34990.9, -132.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos24", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.46], "Focus": [17741.4], "XYStage": [ 29678.100000000002, -958.1 ] }, "GridColumnIndex": 0 }, { "Label": "Pos25", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.44], "Focus": [17741.5], "XYStage": [ 28983.600000000002, -939.4000000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos26", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.475], "Focus": [17741.5], "XYStage": [ 23019.7, -122 ] }, "GridColumnIndex": 0 }, { "Label": "Pos27", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.44], "Focus": [17741.7], "XYStage": [ 22428, -116.80000000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos28", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.46], "Focus": [17741.7], "XYStage": [ 16364.900000000001, -181.10000000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos29", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.48000000000002], "Focus": [17741.5], "XYStage": [ 15712.1, -180 ] }, "GridColumnIndex": 0 }, { "Label": "Pos30", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.45000000000002], "Focus": [17741.7], "XYStage": [ 10346.1, 77.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos31", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.49], "Focus": [17741.4], "XYStage": [ 9755.5, 85.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos32", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.475], "Focus": [17741.5], "XYStage": [ 4775.5, 293.90000000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos33", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.445], "Focus": [17741.4], "XYStage": [ 3954.9, 293.90000000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos34", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.455], "Focus": [17741.4], "XYStage": [ -1324.9, 545.6 ] }, "GridColumnIndex": 0 }, { "Label": "Pos35", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.445], "Focus": [17741.3], "XYStage": [ -2097, 576.6 ] }, "GridColumnIndex": 0 } ], "z-step_um": 10, "GridRow": 0, "BitDepth": 16, "Depth": 2, "Channels": 6, "Source": "Micro-Manager", "ChColors": [ -16711681, -256, -65281, -1, -1, -16776961 ], "MetadataVersion": 10, "KeepShutterOpenChannels": true, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536, 65536 ], "CustomIntervals_ms": [], "Height": 1200, "MicroManagerVersion": "1.4.23 20180219", "GridColumn": 0, "UUID": "18808e7c-f2e6-47b3-90d8-1d779ce87dbd", "PixelType": "GRAY16", "Width": 1200, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\Yan\\20191218-YC113-S7S8-miseq", "Frames": 1, "Comment": "", "UserName": "Nikon", "PixelSize_um": 1.1, "ROI": [ 0, 0, 1200, 1200 ], "Date": "2019-12-19", "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC", "P-DAPI-high" ], "KeepShutterOpenSlices": true, "IJType": 1, "ComputerName": "ZadorConfocal2", "Positions": 36, "PositionIndex": 34, "CameraTimeout": 20000, "TimeFirst": false }, "640 AO-Name": "AnalogIO", "Vincent-D3-Command": "Close" }, "FrameKey-0-3-8": { "COM4-StopBits": "1", "528 AO-MaxVolts": "5.0000", "XLIGHT Excitation Wheel-State": "1", "470 Shutter-Name": "AnalogIO", "Core-Shutter": "640", "555 AO-Demo": "No", "XLIGHT Dichroic-State": "2", "XYStage-StepSize-Y": "0.1000", "ZPositionUm": 248.455, "XYStage-StepSize-X": "0.1000", "PRIME-FirmwareVersion": "20.28", "PRIME-ClearCycles": "2", "520-Name": "DA Shutter", "405 Shutter-Name": "AnalogIO", "PIEZO-StepSize": "0.0050", "PRIME-ClearMode": "Pre-Exposure", "470 AO-MinVolts": "0.0000", "405 AO-SupportsTriggering": "1", "CameraChannelIndex": 0, "XYStage-StartSpeed": "500.0000", "COM9-StopBits": "2", "405 AO-Description": "NI DAC", "COM4-DelayBetweenCharsMs": "0.0000", "445 AO-Block voltage": "false", "520 AO-TriggerInputLine": "/Dev1/PFI0", "555 AO-Description": "NI DAC", "520 AO-TriggerSampleRate": "100000", "COM4-Verbose": "1", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM4-Name": "COM4", "405 Shutter-Volts": "0.0000", "Vincent-D3-Port": "COM1", "PRIME-PP 4 MIN ADU AFFECTED": "200", "470 Shutter-MinVolts": "0.0000", "PRIME-PP 3 ENABLED": "Yes", "470 AO-Description": "NI DAC", "PRIME-Description": "PVCAM API device adapter", "520 Shutter-MaxVolts": "5.0000", "520 Shutter-Volts": "0.0000", "528 AO-Sequenceable": "1", "PRIME-Binning": "1x1", "555 Shutter-MaxVolts": "5.0000", "528 Shutter-TriggerSequenceLength": "1000", "405 Shutter-Block voltage": "false", "XLIGHT Spin motor-State": "1", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "Vincent-D3-Time to open (ms)": "35.0000", "Vincent-D3-Time to close (ms)": "35.0000", "445 AO-TriggerSampleRate": "100000", "640 Shutter-IOChannel": "Dev1/ao14", "555 AO-Sequenceable": "1", "520 AO-Name": "AnalogIO", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "470 AO-Block voltage": "false", "COM1-Verbose": "1", "COM9-AnswerTimeout": "10000.0000", "528 Shutter-Name": "AnalogIO", "PRIME-Trigger Timeout (secs)": "10", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "NextFrame": 0, "470 AO-TriggerSequenceLength": "1000", "PRIME-TransposeXY": "0", "445 AO-TriggerSequenceLength": "1000", "COM1-Name": "COM1", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "COM9-DataBits": "8", "COM1-AnswerTimeout": "500.0000", "Camera": "", "TE2000-Version": "V2.50-V2.50-V2.10", "528 Shutter-Volts": "0.0000", "XLIGHT Hub-Name": "XLIGHT Hub", "PIEZO-Description": "Ludl stage driver adapter", "PositionName": "Pos34", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSampleRate": "100000", "PRIME-FanSpeedSetpoint": "High", "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "405 AO-TriggerSampleRate": "100000", "470 Shutter-Block voltage": "false", "470-State": "0", "COM3-Parity": "None", "405 AO-Volts": "0.0000", "PRIME-TransposeMirrorY": "0", "PRIME-ChipName": "GS144BSI", "470 AO-Volts": "0.0000", "PRIME-TransposeMirrorX": "0", "445 Shutter-SupportsTriggering": "0", "470 Shutter-SupportsTriggering": "0", "XLIGHT Hub-Description": "XLIGHT hub", "PRIME-PreampOffLimit": "10000", "470 AO-Sequenceable": "1", "528 Shutter-MaxVolts": "5.0000", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "Core-XYStage": "XYStage", "470 AO-TriggerSampleRate": "100000", "PRIME-Timing-PreTriggerDelayNs": "24672000.0000", "528-DA Device": "528 Shutter", "Focus-Description": "Nikon TE2000 focus adapter", "FilterBlock-State": "5", "520 AO-Demo": "No", "COM3-DelayBetweenCharsMs": "0.0000", "470 Shutter-Volts": "0.0000", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "PRIME-PP 2 THRESHOLD": "125", "PRIME-PP 4 THRESHOLD": "75", "XYStage-Acceleration": "4.0000", "470 Shutter-Demo": "No", "PRIME-Name": "PMPCIECam00", "640-DA Device": "640 Shutter", "405 AO-Block voltage": "false", "ElapsedTime-ms": 1416512, "PRIME-Offset": "100", "445 AO-IOChannel": "Dev1/ao1", "640 AO-MaxVolts": "5.0000", "PositionIndex": 34, "PRIME-PP 1 ENABLED": "Yes", "COM1-BaudRate": "9600", "640-State": "1", "COM3-Verbose": "1", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "LudlController-TransmissionDelay": "0", "PRIME-FullWellCapacity": "62000", "PRIME-SMARTStreamingEnabled": "No", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "640 Shutter-Description": "NI DAC", "528-Description": "DA device that is used as a shutter", "COM4-Description": "Serial port driver (boost:asio)", "405 Shutter-Description": "NI DAC", "TE2000-Name": "TE2000", "528 Shutter-Description": "NI DAC", "COM9-Description": "Serial port driver (boost:asio)", "LudlController-Name": "LudlController", "445 Shutter-TriggerSequenceLength": "1000", "PRIME-SerialNumber": "A18B203008", "445 AO-Volts": "0.0000", "PRIME-X-dimension": "1200", "PRIME-ShutterCloseDelay": "0", "XLIGHT Disk slider-State": "2", "Position": "Default", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 AO-IOChannel": "Dev1/ao5", "520 AO-IOChannel": "Dev1/ao4", "528 AO-TriggerSequenceLength": "1000", "405 Shutter-MaxVolts": "5.0000", "470-DA Device": "470 Shutter", "PRIME-FTCapable": "No", "Core-ChannelGroup": "Channels", "555 AO-SupportsTriggering": "0", "PRIME-Y-dimension": "1200", "Frame": 0, "555 Shutter-Sequenceable": "1", "PRIME-Timing-ExposureTimeNs": "499998000.0000", "Core-ImageProcessor": "", "528-State": "0", "520 AO-Block voltage": "false", "XLIGHT Excitation Wheel-Label": "QUAD", "640 Shutter-Demo": "No", "COM3-StopBits": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "520 Shutter-IOChannel": "Dev1/ao11", "Width": 1200, "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-SupportsTriggering": "0", "640 AO-TriggerSampleRate": "100000", "520-Description": "DA device that is used as a shutter", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "405 Shutter-Sequenceable": "1", "405 Shutter-SupportsTriggering": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-TriggerSampleRate": "100000", "640 Shutter-MaxVolts": "5.0000", "555 AO-MaxVolts": "5.0000", "520 AO-TriggerSequenceLength": "1000", "COM3-BaudRate": "9600", "COM9-BaudRate": "9600", "Vincent-D3-Channel #": "Ch. #1", "PRIME-PMode": "Normal", "OpticalPath-Label": "Left(100)", "BitDepth": 16, "555 AO-TriggerSequenceLength": "1000", "445 Shutter-Description": "NI DAC", "405 AO-Sequenceable": "1", "PRIME-AcquisitionMethod": "Callbacks", "555 AO-Name": "AnalogIO", "PRIME-ShutterMode": "Pre-Exposure", "COM3-Handshaking": "Off", "445 AO-SupportsTriggering": "0", "ROI": "0-0-1200-1200", "COM9-Verbose": "1", "PRIME-Color": "OFF", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "PRIME-ShutterOpenDelay": "0", "COM1-Parity": "None", "Slice": 8, "SlicePosition": 30, "COM9-DelayBetweenCharsMs": "0.0000", "555-Name": "DA Shutter", "PIEZO-LudlSingleAxisName": "B", "XLIGHT Emission Wheel-Label": "725/40 (C)", "640 Shutter-Name": "AnalogIO", "640 AO-Sequenceable": "1", "520 Shutter-Demo": "No", "528 Shutter-Demo": "No", "470-Name": "DA Shutter", "520-DA Device": "520 Shutter", "XYStage-Description": "Ludl XY stage driver adapter", "445 Shutter-MinVolts": "0.0000", "528 AO-TriggerSampleRate": "100000", "405 AO-MinVolts": "0.0000", "405 Shutter-TriggerSequenceLength": "1000", "Height": 1200, "555 AO-Block voltage": "false", "640 Shutter-Volts": "5.0000", "PixelType": "GRAY16", "445 Shutter-Block voltage": "false", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "520 Shutter-TriggerSequenceLength": "1000", "COM9-Name": "COM9", "Core-SLM": "", "555 AO-Volts": "0.0000", "555 Shutter-Block voltage": "false", "XYStage-Speed": "1800.0000", "520 AO-MaxVolts": "5.0000", "528 Shutter-SupportsTriggering": "0", "COM1-Description": "Serial port driver (boost:asio)", "PRIME-MetadataEnabled": "No", "520 Shutter-MinVolts": "0.0000", "470 Shutter-Sequenceable": "1", "PRIME-PreampDelay": "0", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-MinVolts": "0.0000", "ChannelIndex": 3, "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Core-AutoShutter": "1", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "Exposure-ms": 520, "PRIME-PP 2 ENABLED": "Yes", "640 Shutter-TriggerSampleRate": "100000", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "470 Shutter-IOChannel": "Dev1/ao10", "405 AO-Demo": "No", "PixelSizeUm": 1.1, "470 AO-Demo": "No", "470 Shutter-MaxVolts": "5.0000", "Vincent-D3-Name": "Vincent-D3", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FileName": "img_000000000_Il-C_008.tif", "LudlController-Reset": "Operate", "PRIME-Exposure": "520.00", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "445-Name": "DA Shutter", "555 Shutter-Name": "AnalogIO", "520 Shutter-TriggerSampleRate": "100000", "445 Shutter-IOChannel": "Dev1/ao9", "PRIME-TransposeCorrection": "0", "XYStage-StepSize": "0.1000", "445-Description": "DA device that is used as a shutter", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 AO-IOChannel": "Dev1/ao7", "520 Shutter-Name": "AnalogIO", "COM3-DataBits": "8", "640 AO-Volts": "4.5000", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "555 Shutter-IOChannel": "Dev1/ao13", "WaitInterval": null, "520 Shutter-Sequenceable": "1", "YPositionUm": 545.6, "445-State": "0", "445-DA Device": "445 Shutter", "PRIME-CircularBufferAutoSize": "ON", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "555 AO-TriggerSampleRate": "100000", "445 Shutter-Volts": "0.0000", "XLIGHT Hub-Port": "COM3", "Core-AutoFocus": "", "COM4-Handshaking": "Off", "528 Shutter-MinVolts": "0.0000", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "445 Shutter-Name": "AnalogIO", "528 Shutter-Sequenceable": "1", "XYStage-TransposeMirrorX": "0", "XYStage-TransposeMirrorY": "0", "PRIME-ReadoutRate": "100MHz 16bit", "XYStage-Name": "Stage", "640 AO-SupportsTriggering": "0", "640-Name": "DA Shutter", "640 Shutter-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "640 AO-Block voltage": "false", "445 Shutter-Sequenceable": "1", "470 AO-MaxVolts": "5.0000", "COM4-BaudRate": "9600", "470-Description": "DA device that is used as a shutter", "520 Shutter-Description": "NI DAC", "PRIME-CCDTemperature": "-14.8500", "Time": "2019-12-19 09:59:46 -0500", "640 AO-TriggerSequenceLength": "1000", "470 AO-Name": "AnalogIO", "XLIGHT Disk slider-Label": "Disk Pos 40um", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "Binning": "1x1", "TE2000-ModelType": "1", "COM3-AnswerTimeout": "500.0000", "555 Shutter-SupportsTriggering": "0", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "TE2000-Port": "COM4", "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Description": "NI DAC", "COM4-AnswerTimeout": "500.0000", "COM1-DelayBetweenCharsMs": "0.0000", "FilterBlock-Label": "Confocal", "445 AO-Name": "AnalogIO", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-Timing-ReadoutTimeNs": "24672000.0000", "528 AO-Demo": "No", "520 AO-Sequenceable": "1", "555-Description": "DA device that is used as a shutter", "405-DA Device": "405 Shutter", "405 AO-TriggerSequenceLength": "1000", "640 AO-MinVolts": "0.0000", "640 Shutter-Block voltage": "false", "FrameIndex": 0, "PRIME-Port": "CMOS", "555 AO-MinVolts": "0.0000", "COM3-Description": "Serial port driver (boost:asio)", "528 AO-Description": "NI DAC", "555 AO-IOChannel": "Dev1/ao6", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "555 AO-TriggerInputLine": "/Dev1/PFI0", "PRIME-ActualInterval-ms": "390.5200", "LudlController-Port": "COM9", "445 Shutter-Demo": "No", "PRIME-Trigger-Expose Out-Mux": "1", "SliceIndex": 8, "PRIME-CCDTemperatureSetPoint": "-15.0000", "405 Shutter-MinVolts": "0.0000", "PRIME-ExposeOutMode": "First Row", "AxisPositions": null, "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Volts": "0.0000", "528-Name": "DA Shutter", "555-State": "0", "XLIGHT Dichroic-Label": "Di03-R635-t1", "445 AO-Sequenceable": "1", "405-Name": "DA Shutter", "405 AO-IOChannel": "Dev1/ao0", "Core-Camera": "PRIME", "Focus-Name": "Focus", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "405 Shutter-IOChannel": "Dev1/ao8", "640 AO-Demo": "No", "640 Shutter-SupportsTriggering": "0", "445 AO-Demo": "No", "555-DA Device": "555 Shutter", "470 Shutter-Description": "NI DAC", "528 AO-Volts": "0.0000", "COM1-DataBits": "8", "Vincent-D3-Address": "x", "520 AO-SupportsTriggering": "0", "405-Description": "DA device that is used as a shutter", "640 Shutter-MinVolts": "0.0000", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "405 AO-Name": "AnalogIO", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "640 AO-TriggerInputLine": "/Dev1/PFI0", "405 AO-TriggerInputLine": "/Dev1/PFI0", "640-Description": "DA device that is used as a shutter", "445 Shutter-TriggerSampleRate": "100000", "470 Shutter-TriggerSequenceLength": "1000", "COM1-StopBits": "1", "520 Shutter-SupportsTriggering": "0", "405-State": "0", "Core-Initialize": "1", "XLIGHT Emission Wheel-State": "3", "520 AO-Description": "NI DAC", "528 AO-TriggerInputLine": "/Dev1/PFI0", "445 Shutter-MaxVolts": "5.0000", "COM9-Handshaking": "Off", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "XLIGHT Spin motor-Label": "On (spinning)", "PRIME-PP 5 ENABLED": "No", "UUID": "81c541ae-53e8-4224-9524-409ecfca0f64", "PRIME-TriggerMode": "Internal Trigger", "COM1-Handshaking": "Off", "PRIME-Timing-PostTriggerDelayNs": "49344000.0000", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "Focus-Position": "17741.5000", "528 Shutter-Block voltage": "false", "Channel": "Il-C", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "555 Shutter-Demo": "No", "405 Shutter-Demo": "No", "528 AO-Name": "AnalogIO", "520-State": "0", "Core-Galvo": "", "405 Shutter-TriggerSampleRate": "100000", "Focus-FocusStepSize_(nm)": "100", "PRIME-PixelType": "16bit", "COM4-DataBits": "8", "470 AO-IOChannel": "Dev1/ao2", "520 AO-MinVolts": "0.0000", "COM9-Parity": "None", "PRIME-CircularBufferEnabled": "ON", "PRIME-PP 6 Reset": "No", "640 AO-Description": "NI DAC", "Source": "PRIME", "445 AO-MinVolts": "0.0000", "PIEZO-Name": "Stage", "445 AO-Description": "NI DAC", "PRIME-CircularBufferFrameCount": "46", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "520 AO-Volts": "0.0000", "528 Shutter-TriggerSampleRate": "100000", "XPositionUm": -1324.9, "OpticalPath-State": "4", "PRIME-PP 3 THRESHOLD": "80", "PRIME-PVCAM Version": "3.7.1", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Gain": "1-HDR", "640 Shutter-TriggerSequenceLength": "1000", "528 AO-SupportsTriggering": "0", "445 AO-TriggerInputLine": "/Dev1/PFI0", "OpticalPath-Name": "OpticalPath", "PRIME-PP 4 ENABLED": "Yes", "PRIME-PVCAM Adapter Version": "1.3.29", "COM3-Name": "COM3", "405 AO-MaxVolts": "5.0000", "520 Shutter-Block voltage": "false", "PRIME-Timing-ClearingTimeNs": "49344000.0000", "Core-Focus": "PIEZO", "528 AO-Block voltage": "false", "TE2000-Description": "Nikon TE2000 hub", "528 AO-MinVolts": "0.0000", "COM4-Parity": "None", "Summary": { "Slices": 11, "ChContrastMin": [ 0, 0, 0, 0, 0, 0 ], "PixelAspect": 1, "SlicesFirst": true, "Interval_ms": 0, "Time": "2019-12-19 09:59:21 -0500", "Prefix": "seq_13", "InitialPositionList": [ { "Label": "Pos0", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.21], "Focus": [17741.5], "XYStage": [ 44542.200000000004, 31636.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos1", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.22], "Focus": [17741.4], "XYStage": [ 43801.100000000006, 31637.9 ] }, "GridColumnIndex": 0 }, { "Label": "Pos2", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.5], "XYStage": [ 38561.3, 32040.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos3", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.185], "Focus": [17741.3], "XYStage": [ 37698.4, 32041.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos4", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.16], "Focus": [17741.5], "XYStage": [ 32375.4, 31758.9 ] }, "GridColumnIndex": 0 }, { "Label": "Pos5", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.17000000000002], "Focus": [17741.3], "XYStage": [ 31770.7, 31758.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos6", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.4], "XYStage": [ 26536.800000000003, 31068.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos7", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.205], "Focus": [17741.4], "XYStage": [ 25420.2, 31068.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos8", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.85], "Focus": [17741.3], "XYStage": [ 21059, 31508 ] }, "GridColumnIndex": 0 }, { "Label": "Pos9", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.82], "Focus": [17741.3], "XYStage": [ 19731.9, 31506.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos10", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.79], "Focus": [17741.4], "XYStage": [ 14528.5, 32007.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos11", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.83], "Focus": [17741.2], "XYStage": [ 13830.300000000001, 32009.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos12", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.79], "Focus": [17741.3], "XYStage": [ 8775.300000000001, 32017.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos13", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.815], "Focus": [17741.3], "XYStage": [ 8109.200000000001, 32008.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos14", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.805], "Focus": [17741.3], "XYStage": [ 3483.3, 31908.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos15", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.82], "Focus": [17741.2], "XYStage": [ 2686.8, 31903.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos16", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [211.48000000000002], "Focus": [17741.1], "XYStage": [ -1978.9, 31973.600000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos17", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [211.51500000000001], "Focus": [17741.1], "XYStage": [ -2957.8, 31982.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos18", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.195], "Focus": [17741.5], "XYStage": [ 47747.4, -576.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos19", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.23000000000002], "Focus": [17741.6], "XYStage": [ 47053.9, -576.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos20", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.205], "Focus": [17741.4], "XYStage": [ 41871.200000000004, -479.8 ] }, "GridColumnIndex": 0 }, { "Label": "Pos21", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.4], "XYStage": [ 40882.600000000006, -392 ] }, "GridColumnIndex": 0 }, { "Label": "Pos22", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.24], "Focus": [17741.4], "XYStage": [ 35905.6, -135.3 ] }, "GridColumnIndex": 0 }, { "Label": "Pos23", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.195], "Focus": [17741.5], "XYStage": [ 34990.9, -132.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos24", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.46], "Focus": [17741.4], "XYStage": [ 29678.100000000002, -958.1 ] }, "GridColumnIndex": 0 }, { "Label": "Pos25", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.44], "Focus": [17741.5], "XYStage": [ 28983.600000000002, -939.4000000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos26", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.475], "Focus": [17741.5], "XYStage": [ 23019.7, -122 ] }, "GridColumnIndex": 0 }, { "Label": "Pos27", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.44], "Focus": [17741.7], "XYStage": [ 22428, -116.80000000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos28", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.46], "Focus": [17741.7], "XYStage": [ 16364.900000000001, -181.10000000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos29", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.48000000000002], "Focus": [17741.5], "XYStage": [ 15712.1, -180 ] }, "GridColumnIndex": 0 }, { "Label": "Pos30", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.45000000000002], "Focus": [17741.7], "XYStage": [ 10346.1, 77.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos31", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.49], "Focus": [17741.4], "XYStage": [ 9755.5, 85.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos32", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.475], "Focus": [17741.5], "XYStage": [ 4775.5, 293.90000000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos33", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.445], "Focus": [17741.4], "XYStage": [ 3954.9, 293.90000000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos34", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.455], "Focus": [17741.4], "XYStage": [ -1324.9, 545.6 ] }, "GridColumnIndex": 0 }, { "Label": "Pos35", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.445], "Focus": [17741.3], "XYStage": [ -2097, 576.6 ] }, "GridColumnIndex": 0 } ], "z-step_um": 10, "GridRow": 0, "BitDepth": 16, "Depth": 2, "Channels": 6, "Source": "Micro-Manager", "ChColors": [ -16711681, -256, -65281, -1, -1, -16776961 ], "MetadataVersion": 10, "KeepShutterOpenChannels": true, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536, 65536 ], "CustomIntervals_ms": [], "Height": 1200, "MicroManagerVersion": "1.4.23 20180219", "GridColumn": 0, "UUID": "18808e7c-f2e6-47b3-90d8-1d779ce87dbd", "PixelType": "GRAY16", "Width": 1200, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\Yan\\20191218-YC113-S7S8-miseq", "Frames": 1, "Comment": "", "UserName": "Nikon", "PixelSize_um": 1.1, "ROI": [ 0, 0, 1200, 1200 ], "Date": "2019-12-19", "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC", "P-DAPI-high" ], "KeepShutterOpenSlices": true, "IJType": 1, "ComputerName": "ZadorConfocal2", "Positions": 36, "PositionIndex": 34, "CameraTimeout": 20000, "TimeFirst": false }, "640 AO-Name": "AnalogIO", "Vincent-D3-Command": "Close" }, "FrameKey-0-3-9": { "COM4-StopBits": "1", "528 AO-MaxVolts": "5.0000", "XLIGHT Excitation Wheel-State": "1", "470 Shutter-Name": "AnalogIO", "Core-Shutter": "640", "555 AO-Demo": "No", "XLIGHT Dichroic-State": "2", "XYStage-StepSize-Y": "0.1000", "ZPositionUm": 258.45500000000004, "XYStage-StepSize-X": "0.1000", "PRIME-FirmwareVersion": "20.28", "PRIME-ClearCycles": "2", "520-Name": "DA Shutter", "405 Shutter-Name": "AnalogIO", "PIEZO-StepSize": "0.0050", "PRIME-ClearMode": "Pre-Exposure", "470 AO-MinVolts": "0.0000", "405 AO-SupportsTriggering": "1", "CameraChannelIndex": 0, "XYStage-StartSpeed": "500.0000", "COM9-StopBits": "2", "405 AO-Description": "NI DAC", "COM4-DelayBetweenCharsMs": "0.0000", "445 AO-Block voltage": "false", "520 AO-TriggerInputLine": "/Dev1/PFI0", "555 AO-Description": "NI DAC", "520 AO-TriggerSampleRate": "100000", "COM4-Verbose": "1", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM4-Name": "COM4", "405 Shutter-Volts": "0.0000", "Vincent-D3-Port": "COM1", "PRIME-PP 4 MIN ADU AFFECTED": "200", "470 Shutter-MinVolts": "0.0000", "PRIME-PP 3 ENABLED": "Yes", "470 AO-Description": "NI DAC", "PRIME-Description": "PVCAM API device adapter", "520 Shutter-MaxVolts": "5.0000", "520 Shutter-Volts": "0.0000", "528 AO-Sequenceable": "1", "PRIME-Binning": "1x1", "555 Shutter-MaxVolts": "5.0000", "528 Shutter-TriggerSequenceLength": "1000", "405 Shutter-Block voltage": "false", "XLIGHT Spin motor-State": "1", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "Vincent-D3-Time to open (ms)": "35.0000", "Vincent-D3-Time to close (ms)": "35.0000", "445 AO-TriggerSampleRate": "100000", "640 Shutter-IOChannel": "Dev1/ao14", "555 AO-Sequenceable": "1", "520 AO-Name": "AnalogIO", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "470 AO-Block voltage": "false", "COM1-Verbose": "1", "COM9-AnswerTimeout": "10000.0000", "528 Shutter-Name": "AnalogIO", "PRIME-Trigger Timeout (secs)": "10", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "NextFrame": 0, "470 AO-TriggerSequenceLength": "1000", "PRIME-TransposeXY": "0", "445 AO-TriggerSequenceLength": "1000", "COM1-Name": "COM1", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "COM9-DataBits": "8", "COM1-AnswerTimeout": "500.0000", "Camera": "", "TE2000-Version": "V2.50-V2.50-V2.10", "528 Shutter-Volts": "0.0000", "XLIGHT Hub-Name": "XLIGHT Hub", "PIEZO-Description": "Ludl stage driver adapter", "PositionName": "Pos34", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSampleRate": "100000", "PRIME-FanSpeedSetpoint": "High", "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "405 AO-TriggerSampleRate": "100000", "470 Shutter-Block voltage": "false", "470-State": "0", "COM3-Parity": "None", "405 AO-Volts": "0.0000", "PRIME-TransposeMirrorY": "0", "PRIME-ChipName": "GS144BSI", "470 AO-Volts": "0.0000", "PRIME-TransposeMirrorX": "0", "445 Shutter-SupportsTriggering": "0", "470 Shutter-SupportsTriggering": "0", "XLIGHT Hub-Description": "XLIGHT hub", "PRIME-PreampOffLimit": "10000", "470 AO-Sequenceable": "1", "528 Shutter-MaxVolts": "5.0000", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "Core-XYStage": "XYStage", "470 AO-TriggerSampleRate": "100000", "PRIME-Timing-PreTriggerDelayNs": "24672000.0000", "528-DA Device": "528 Shutter", "Focus-Description": "Nikon TE2000 focus adapter", "FilterBlock-State": "5", "520 AO-Demo": "No", "COM3-DelayBetweenCharsMs": "0.0000", "470 Shutter-Volts": "0.0000", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "PRIME-PP 2 THRESHOLD": "125", "PRIME-PP 4 THRESHOLD": "75", "XYStage-Acceleration": "4.0000", "470 Shutter-Demo": "No", "PRIME-Name": "PMPCIECam00", "640-DA Device": "640 Shutter", "405 AO-Block voltage": "false", "ElapsedTime-ms": 1417268, "PRIME-Offset": "100", "445 AO-IOChannel": "Dev1/ao1", "640 AO-MaxVolts": "5.0000", "PositionIndex": 34, "PRIME-PP 1 ENABLED": "Yes", "COM1-BaudRate": "9600", "640-State": "1", "COM3-Verbose": "1", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "LudlController-TransmissionDelay": "0", "PRIME-FullWellCapacity": "62000", "PRIME-SMARTStreamingEnabled": "No", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "640 Shutter-Description": "NI DAC", "528-Description": "DA device that is used as a shutter", "COM4-Description": "Serial port driver (boost:asio)", "405 Shutter-Description": "NI DAC", "TE2000-Name": "TE2000", "528 Shutter-Description": "NI DAC", "COM9-Description": "Serial port driver (boost:asio)", "LudlController-Name": "LudlController", "445 Shutter-TriggerSequenceLength": "1000", "PRIME-SerialNumber": "A18B203008", "445 AO-Volts": "0.0000", "PRIME-X-dimension": "1200", "PRIME-ShutterCloseDelay": "0", "XLIGHT Disk slider-State": "2", "Position": "Default", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 AO-IOChannel": "Dev1/ao5", "520 AO-IOChannel": "Dev1/ao4", "528 AO-TriggerSequenceLength": "1000", "405 Shutter-MaxVolts": "5.0000", "470-DA Device": "470 Shutter", "PRIME-FTCapable": "No", "Core-ChannelGroup": "Channels", "555 AO-SupportsTriggering": "0", "PRIME-Y-dimension": "1200", "Frame": 0, "555 Shutter-Sequenceable": "1", "PRIME-Timing-ExposureTimeNs": "499998000.0000", "Core-ImageProcessor": "", "528-State": "0", "520 AO-Block voltage": "false", "XLIGHT Excitation Wheel-Label": "QUAD", "640 Shutter-Demo": "No", "COM3-StopBits": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "520 Shutter-IOChannel": "Dev1/ao11", "Width": 1200, "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-SupportsTriggering": "0", "640 AO-TriggerSampleRate": "100000", "520-Description": "DA device that is used as a shutter", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "405 Shutter-Sequenceable": "1", "405 Shutter-SupportsTriggering": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-TriggerSampleRate": "100000", "640 Shutter-MaxVolts": "5.0000", "555 AO-MaxVolts": "5.0000", "520 AO-TriggerSequenceLength": "1000", "COM3-BaudRate": "9600", "COM9-BaudRate": "9600", "Vincent-D3-Channel #": "Ch. #1", "PRIME-PMode": "Normal", "OpticalPath-Label": "Left(100)", "BitDepth": 16, "555 AO-TriggerSequenceLength": "1000", "445 Shutter-Description": "NI DAC", "405 AO-Sequenceable": "1", "PRIME-AcquisitionMethod": "Callbacks", "555 AO-Name": "AnalogIO", "PRIME-ShutterMode": "Pre-Exposure", "COM3-Handshaking": "Off", "445 AO-SupportsTriggering": "0", "ROI": "0-0-1200-1200", "COM9-Verbose": "1", "PRIME-Color": "OFF", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "PRIME-ShutterOpenDelay": "0", "COM1-Parity": "None", "Slice": 9, "SlicePosition": 40, "COM9-DelayBetweenCharsMs": "0.0000", "555-Name": "DA Shutter", "PIEZO-LudlSingleAxisName": "B", "XLIGHT Emission Wheel-Label": "725/40 (C)", "640 Shutter-Name": "AnalogIO", "640 AO-Sequenceable": "1", "520 Shutter-Demo": "No", "528 Shutter-Demo": "No", "470-Name": "DA Shutter", "520-DA Device": "520 Shutter", "XYStage-Description": "Ludl XY stage driver adapter", "445 Shutter-MinVolts": "0.0000", "528 AO-TriggerSampleRate": "100000", "405 AO-MinVolts": "0.0000", "405 Shutter-TriggerSequenceLength": "1000", "Height": 1200, "555 AO-Block voltage": "false", "640 Shutter-Volts": "5.0000", "PixelType": "GRAY16", "445 Shutter-Block voltage": "false", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "520 Shutter-TriggerSequenceLength": "1000", "COM9-Name": "COM9", "Core-SLM": "", "555 AO-Volts": "0.0000", "555 Shutter-Block voltage": "false", "XYStage-Speed": "1800.0000", "520 AO-MaxVolts": "5.0000", "528 Shutter-SupportsTriggering": "0", "COM1-Description": "Serial port driver (boost:asio)", "PRIME-MetadataEnabled": "No", "520 Shutter-MinVolts": "0.0000", "470 Shutter-Sequenceable": "1", "PRIME-PreampDelay": "0", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-MinVolts": "0.0000", "ChannelIndex": 3, "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Core-AutoShutter": "1", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "Exposure-ms": 520, "PRIME-PP 2 ENABLED": "Yes", "640 Shutter-TriggerSampleRate": "100000", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "470 Shutter-IOChannel": "Dev1/ao10", "405 AO-Demo": "No", "PixelSizeUm": 1.1, "470 AO-Demo": "No", "470 Shutter-MaxVolts": "5.0000", "Vincent-D3-Name": "Vincent-D3", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FileName": "img_000000000_Il-C_009.tif", "LudlController-Reset": "Operate", "PRIME-Exposure": "520.00", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "445-Name": "DA Shutter", "555 Shutter-Name": "AnalogIO", "520 Shutter-TriggerSampleRate": "100000", "445 Shutter-IOChannel": "Dev1/ao9", "PRIME-TransposeCorrection": "0", "XYStage-StepSize": "0.1000", "445-Description": "DA device that is used as a shutter", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 AO-IOChannel": "Dev1/ao7", "520 Shutter-Name": "AnalogIO", "COM3-DataBits": "8", "640 AO-Volts": "4.5000", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "555 Shutter-IOChannel": "Dev1/ao13", "WaitInterval": null, "520 Shutter-Sequenceable": "1", "YPositionUm": 545.6, "445-State": "0", "445-DA Device": "445 Shutter", "PRIME-CircularBufferAutoSize": "ON", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "555 AO-TriggerSampleRate": "100000", "445 Shutter-Volts": "0.0000", "XLIGHT Hub-Port": "COM3", "Core-AutoFocus": "", "COM4-Handshaking": "Off", "528 Shutter-MinVolts": "0.0000", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "445 Shutter-Name": "AnalogIO", "528 Shutter-Sequenceable": "1", "XYStage-TransposeMirrorX": "0", "XYStage-TransposeMirrorY": "0", "PRIME-ReadoutRate": "100MHz 16bit", "XYStage-Name": "Stage", "640 AO-SupportsTriggering": "0", "640-Name": "DA Shutter", "640 Shutter-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "640 AO-Block voltage": "false", "445 Shutter-Sequenceable": "1", "470 AO-MaxVolts": "5.0000", "COM4-BaudRate": "9600", "470-Description": "DA device that is used as a shutter", "520 Shutter-Description": "NI DAC", "PRIME-CCDTemperature": "-14.8500", "Time": "2019-12-19 09:59:47 -0500", "640 AO-TriggerSequenceLength": "1000", "470 AO-Name": "AnalogIO", "XLIGHT Disk slider-Label": "Disk Pos 40um", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "Binning": "1x1", "TE2000-ModelType": "1", "COM3-AnswerTimeout": "500.0000", "555 Shutter-SupportsTriggering": "0", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "TE2000-Port": "COM4", "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Description": "NI DAC", "COM4-AnswerTimeout": "500.0000", "COM1-DelayBetweenCharsMs": "0.0000", "FilterBlock-Label": "Confocal", "445 AO-Name": "AnalogIO", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-Timing-ReadoutTimeNs": "24672000.0000", "528 AO-Demo": "No", "520 AO-Sequenceable": "1", "555-Description": "DA device that is used as a shutter", "405-DA Device": "405 Shutter", "405 AO-TriggerSequenceLength": "1000", "640 AO-MinVolts": "0.0000", "640 Shutter-Block voltage": "false", "FrameIndex": 0, "PRIME-Port": "CMOS", "555 AO-MinVolts": "0.0000", "COM3-Description": "Serial port driver (boost:asio)", "528 AO-Description": "NI DAC", "555 AO-IOChannel": "Dev1/ao6", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "555 AO-TriggerInputLine": "/Dev1/PFI0", "PRIME-ActualInterval-ms": "390.5200", "LudlController-Port": "COM9", "445 Shutter-Demo": "No", "PRIME-Trigger-Expose Out-Mux": "1", "SliceIndex": 9, "PRIME-CCDTemperatureSetPoint": "-15.0000", "405 Shutter-MinVolts": "0.0000", "PRIME-ExposeOutMode": "First Row", "AxisPositions": null, "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Volts": "0.0000", "528-Name": "DA Shutter", "555-State": "0", "XLIGHT Dichroic-Label": "Di03-R635-t1", "445 AO-Sequenceable": "1", "405-Name": "DA Shutter", "405 AO-IOChannel": "Dev1/ao0", "Core-Camera": "PRIME", "Focus-Name": "Focus", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "405 Shutter-IOChannel": "Dev1/ao8", "640 AO-Demo": "No", "640 Shutter-SupportsTriggering": "0", "445 AO-Demo": "No", "555-DA Device": "555 Shutter", "470 Shutter-Description": "NI DAC", "528 AO-Volts": "0.0000", "COM1-DataBits": "8", "Vincent-D3-Address": "x", "520 AO-SupportsTriggering": "0", "405-Description": "DA device that is used as a shutter", "640 Shutter-MinVolts": "0.0000", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "405 AO-Name": "AnalogIO", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "640 AO-TriggerInputLine": "/Dev1/PFI0", "405 AO-TriggerInputLine": "/Dev1/PFI0", "640-Description": "DA device that is used as a shutter", "445 Shutter-TriggerSampleRate": "100000", "470 Shutter-TriggerSequenceLength": "1000", "COM1-StopBits": "1", "520 Shutter-SupportsTriggering": "0", "405-State": "0", "Core-Initialize": "1", "XLIGHT Emission Wheel-State": "3", "520 AO-Description": "NI DAC", "528 AO-TriggerInputLine": "/Dev1/PFI0", "445 Shutter-MaxVolts": "5.0000", "COM9-Handshaking": "Off", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "XLIGHT Spin motor-Label": "On (spinning)", "PRIME-PP 5 ENABLED": "No", "UUID": "c9cc270c-8a69-4074-ad18-56056fdb5359", "PRIME-TriggerMode": "Internal Trigger", "COM1-Handshaking": "Off", "PRIME-Timing-PostTriggerDelayNs": "49344000.0000", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "Focus-Position": "17741.5000", "528 Shutter-Block voltage": "false", "Channel": "Il-C", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "555 Shutter-Demo": "No", "405 Shutter-Demo": "No", "528 AO-Name": "AnalogIO", "520-State": "0", "Core-Galvo": "", "405 Shutter-TriggerSampleRate": "100000", "Focus-FocusStepSize_(nm)": "100", "PRIME-PixelType": "16bit", "COM4-DataBits": "8", "470 AO-IOChannel": "Dev1/ao2", "520 AO-MinVolts": "0.0000", "COM9-Parity": "None", "PRIME-CircularBufferEnabled": "ON", "PRIME-PP 6 Reset": "No", "640 AO-Description": "NI DAC", "Source": "PRIME", "445 AO-MinVolts": "0.0000", "PIEZO-Name": "Stage", "445 AO-Description": "NI DAC", "PRIME-CircularBufferFrameCount": "46", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "520 AO-Volts": "0.0000", "528 Shutter-TriggerSampleRate": "100000", "XPositionUm": -1324.9, "OpticalPath-State": "4", "PRIME-PP 3 THRESHOLD": "80", "PRIME-PVCAM Version": "3.7.1", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Gain": "1-HDR", "640 Shutter-TriggerSequenceLength": "1000", "528 AO-SupportsTriggering": "0", "445 AO-TriggerInputLine": "/Dev1/PFI0", "OpticalPath-Name": "OpticalPath", "PRIME-PP 4 ENABLED": "Yes", "PRIME-PVCAM Adapter Version": "1.3.29", "COM3-Name": "COM3", "405 AO-MaxVolts": "5.0000", "520 Shutter-Block voltage": "false", "PRIME-Timing-ClearingTimeNs": "49344000.0000", "Core-Focus": "PIEZO", "528 AO-Block voltage": "false", "TE2000-Description": "Nikon TE2000 hub", "528 AO-MinVolts": "0.0000", "COM4-Parity": "None", "Summary": { "Slices": 11, "ChContrastMin": [ 0, 0, 0, 0, 0, 0 ], "PixelAspect": 1, "SlicesFirst": true, "Interval_ms": 0, "Time": "2019-12-19 09:59:21 -0500", "Prefix": "seq_13", "InitialPositionList": [ { "Label": "Pos0", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.21], "Focus": [17741.5], "XYStage": [ 44542.200000000004, 31636.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos1", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.22], "Focus": [17741.4], "XYStage": [ 43801.100000000006, 31637.9 ] }, "GridColumnIndex": 0 }, { "Label": "Pos2", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.5], "XYStage": [ 38561.3, 32040.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos3", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.185], "Focus": [17741.3], "XYStage": [ 37698.4, 32041.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos4", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.16], "Focus": [17741.5], "XYStage": [ 32375.4, 31758.9 ] }, "GridColumnIndex": 0 }, { "Label": "Pos5", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.17000000000002], "Focus": [17741.3], "XYStage": [ 31770.7, 31758.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos6", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.4], "XYStage": [ 26536.800000000003, 31068.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos7", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.205], "Focus": [17741.4], "XYStage": [ 25420.2, 31068.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos8", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.85], "Focus": [17741.3], "XYStage": [ 21059, 31508 ] }, "GridColumnIndex": 0 }, { "Label": "Pos9", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.82], "Focus": [17741.3], "XYStage": [ 19731.9, 31506.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos10", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.79], "Focus": [17741.4], "XYStage": [ 14528.5, 32007.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos11", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.83], "Focus": [17741.2], "XYStage": [ 13830.300000000001, 32009.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos12", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.79], "Focus": [17741.3], "XYStage": [ 8775.300000000001, 32017.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos13", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.815], "Focus": [17741.3], "XYStage": [ 8109.200000000001, 32008.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos14", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.805], "Focus": [17741.3], "XYStage": [ 3483.3, 31908.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos15", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.82], "Focus": [17741.2], "XYStage": [ 2686.8, 31903.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos16", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [211.48000000000002], "Focus": [17741.1], "XYStage": [ -1978.9, 31973.600000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos17", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [211.51500000000001], "Focus": [17741.1], "XYStage": [ -2957.8, 31982.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos18", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.195], "Focus": [17741.5], "XYStage": [ 47747.4, -576.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos19", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.23000000000002], "Focus": [17741.6], "XYStage": [ 47053.9, -576.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos20", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.205], "Focus": [17741.4], "XYStage": [ 41871.200000000004, -479.8 ] }, "GridColumnIndex": 0 }, { "Label": "Pos21", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.4], "XYStage": [ 40882.600000000006, -392 ] }, "GridColumnIndex": 0 }, { "Label": "Pos22", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.24], "Focus": [17741.4], "XYStage": [ 35905.6, -135.3 ] }, "GridColumnIndex": 0 }, { "Label": "Pos23", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.195], "Focus": [17741.5], "XYStage": [ 34990.9, -132.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos24", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.46], "Focus": [17741.4], "XYStage": [ 29678.100000000002, -958.1 ] }, "GridColumnIndex": 0 }, { "Label": "Pos25", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.44], "Focus": [17741.5], "XYStage": [ 28983.600000000002, -939.4000000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos26", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.475], "Focus": [17741.5], "XYStage": [ 23019.7, -122 ] }, "GridColumnIndex": 0 }, { "Label": "Pos27", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.44], "Focus": [17741.7], "XYStage": [ 22428, -116.80000000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos28", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.46], "Focus": [17741.7], "XYStage": [ 16364.900000000001, -181.10000000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos29", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.48000000000002], "Focus": [17741.5], "XYStage": [ 15712.1, -180 ] }, "GridColumnIndex": 0 }, { "Label": "Pos30", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.45000000000002], "Focus": [17741.7], "XYStage": [ 10346.1, 77.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos31", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.49], "Focus": [17741.4], "XYStage": [ 9755.5, 85.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos32", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.475], "Focus": [17741.5], "XYStage": [ 4775.5, 293.90000000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos33", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.445], "Focus": [17741.4], "XYStage": [ 3954.9, 293.90000000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos34", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.455], "Focus": [17741.4], "XYStage": [ -1324.9, 545.6 ] }, "GridColumnIndex": 0 }, { "Label": "Pos35", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.445], "Focus": [17741.3], "XYStage": [ -2097, 576.6 ] }, "GridColumnIndex": 0 } ], "z-step_um": 10, "GridRow": 0, "BitDepth": 16, "Depth": 2, "Channels": 6, "Source": "Micro-Manager", "ChColors": [ -16711681, -256, -65281, -1, -1, -16776961 ], "MetadataVersion": 10, "KeepShutterOpenChannels": true, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536, 65536 ], "CustomIntervals_ms": [], "Height": 1200, "MicroManagerVersion": "1.4.23 20180219", "GridColumn": 0, "UUID": "18808e7c-f2e6-47b3-90d8-1d779ce87dbd", "PixelType": "GRAY16", "Width": 1200, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\Yan\\20191218-YC113-S7S8-miseq", "Frames": 1, "Comment": "", "UserName": "Nikon", "PixelSize_um": 1.1, "ROI": [ 0, 0, 1200, 1200 ], "Date": "2019-12-19", "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC", "P-DAPI-high" ], "KeepShutterOpenSlices": true, "IJType": 1, "ComputerName": "ZadorConfocal2", "Positions": 36, "PositionIndex": 34, "CameraTimeout": 20000, "TimeFirst": false }, "640 AO-Name": "AnalogIO", "Vincent-D3-Command": "Close" }, "FrameKey-0-3-10": { "COM4-StopBits": "1", "528 AO-MaxVolts": "5.0000", "XLIGHT Excitation Wheel-State": "1", "470 Shutter-Name": "AnalogIO", "Core-Shutter": "640", "555 AO-Demo": "No", "XLIGHT Dichroic-State": "2", "XYStage-StepSize-Y": "0.1000", "ZPositionUm": 268.45500000000004, "XYStage-StepSize-X": "0.1000", "PRIME-FirmwareVersion": "20.28", "PRIME-ClearCycles": "2", "520-Name": "DA Shutter", "405 Shutter-Name": "AnalogIO", "PIEZO-StepSize": "0.0050", "PRIME-ClearMode": "Pre-Exposure", "470 AO-MinVolts": "0.0000", "405 AO-SupportsTriggering": "1", "CameraChannelIndex": 0, "XYStage-StartSpeed": "500.0000", "COM9-StopBits": "2", "405 AO-Description": "NI DAC", "COM4-DelayBetweenCharsMs": "0.0000", "445 AO-Block voltage": "false", "520 AO-TriggerInputLine": "/Dev1/PFI0", "555 AO-Description": "NI DAC", "520 AO-TriggerSampleRate": "100000", "COM4-Verbose": "1", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM4-Name": "COM4", "405 Shutter-Volts": "0.0000", "Vincent-D3-Port": "COM1", "PRIME-PP 4 MIN ADU AFFECTED": "200", "470 Shutter-MinVolts": "0.0000", "PRIME-PP 3 ENABLED": "Yes", "470 AO-Description": "NI DAC", "PRIME-Description": "PVCAM API device adapter", "520 Shutter-MaxVolts": "5.0000", "520 Shutter-Volts": "0.0000", "528 AO-Sequenceable": "1", "PRIME-Binning": "1x1", "555 Shutter-MaxVolts": "5.0000", "528 Shutter-TriggerSequenceLength": "1000", "405 Shutter-Block voltage": "false", "XLIGHT Spin motor-State": "1", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "Vincent-D3-Time to open (ms)": "35.0000", "Vincent-D3-Time to close (ms)": "35.0000", "445 AO-TriggerSampleRate": "100000", "640 Shutter-IOChannel": "Dev1/ao14", "555 AO-Sequenceable": "1", "520 AO-Name": "AnalogIO", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "470 AO-Block voltage": "false", "COM1-Verbose": "1", "COM9-AnswerTimeout": "10000.0000", "528 Shutter-Name": "AnalogIO", "PRIME-Trigger Timeout (secs)": "10", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "NextFrame": 0, "470 AO-TriggerSequenceLength": "1000", "PRIME-TransposeXY": "0", "445 AO-TriggerSequenceLength": "1000", "COM1-Name": "COM1", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "COM9-DataBits": "8", "COM1-AnswerTimeout": "500.0000", "Camera": "", "TE2000-Version": "V2.50-V2.50-V2.10", "528 Shutter-Volts": "0.0000", "XLIGHT Hub-Name": "XLIGHT Hub", "PIEZO-Description": "Ludl stage driver adapter", "PositionName": "Pos34", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSampleRate": "100000", "PRIME-FanSpeedSetpoint": "High", "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "405 AO-TriggerSampleRate": "100000", "470 Shutter-Block voltage": "false", "470-State": "0", "COM3-Parity": "None", "405 AO-Volts": "0.0000", "PRIME-TransposeMirrorY": "0", "PRIME-ChipName": "GS144BSI", "470 AO-Volts": "0.0000", "PRIME-TransposeMirrorX": "0", "445 Shutter-SupportsTriggering": "0", "470 Shutter-SupportsTriggering": "0", "XLIGHT Hub-Description": "XLIGHT hub", "PRIME-PreampOffLimit": "10000", "470 AO-Sequenceable": "1", "528 Shutter-MaxVolts": "5.0000", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "Core-XYStage": "XYStage", "470 AO-TriggerSampleRate": "100000", "PRIME-Timing-PreTriggerDelayNs": "24672000.0000", "528-DA Device": "528 Shutter", "Focus-Description": "Nikon TE2000 focus adapter", "FilterBlock-State": "5", "520 AO-Demo": "No", "COM3-DelayBetweenCharsMs": "0.0000", "470 Shutter-Volts": "0.0000", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "PRIME-PP 2 THRESHOLD": "125", "PRIME-PP 4 THRESHOLD": "75", "XYStage-Acceleration": "4.0000", "470 Shutter-Demo": "No", "PRIME-Name": "PMPCIECam00", "640-DA Device": "640 Shutter", "405 AO-Block voltage": "false", "ElapsedTime-ms": 1418086, "PRIME-Offset": "100", "445 AO-IOChannel": "Dev1/ao1", "640 AO-MaxVolts": "5.0000", "PositionIndex": 34, "PRIME-PP 1 ENABLED": "Yes", "COM1-BaudRate": "9600", "640-State": "0", "COM3-Verbose": "1", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "LudlController-TransmissionDelay": "0", "PRIME-FullWellCapacity": "62000", "PRIME-SMARTStreamingEnabled": "No", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "640 Shutter-Description": "NI DAC", "528-Description": "DA device that is used as a shutter", "COM4-Description": "Serial port driver (boost:asio)", "405 Shutter-Description": "NI DAC", "TE2000-Name": "TE2000", "528 Shutter-Description": "NI DAC", "COM9-Description": "Serial port driver (boost:asio)", "LudlController-Name": "LudlController", "445 Shutter-TriggerSequenceLength": "1000", "PRIME-SerialNumber": "A18B203008", "445 AO-Volts": "0.0000", "PRIME-X-dimension": "1200", "PRIME-ShutterCloseDelay": "0", "XLIGHT Disk slider-State": "2", "Position": "Default", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 AO-IOChannel": "Dev1/ao5", "520 AO-IOChannel": "Dev1/ao4", "528 AO-TriggerSequenceLength": "1000", "405 Shutter-MaxVolts": "5.0000", "470-DA Device": "470 Shutter", "PRIME-FTCapable": "No", "Core-ChannelGroup": "Channels", "555 AO-SupportsTriggering": "0", "PRIME-Y-dimension": "1200", "Frame": 0, "555 Shutter-Sequenceable": "1", "PRIME-Timing-ExposureTimeNs": "499998000.0000", "Core-ImageProcessor": "", "528-State": "0", "520 AO-Block voltage": "false", "XLIGHT Excitation Wheel-Label": "QUAD", "640 Shutter-Demo": "No", "COM3-StopBits": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "520 Shutter-IOChannel": "Dev1/ao11", "Width": 1200, "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-SupportsTriggering": "0", "640 AO-TriggerSampleRate": "100000", "520-Description": "DA device that is used as a shutter", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "405 Shutter-Sequenceable": "1", "405 Shutter-SupportsTriggering": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-TriggerSampleRate": "100000", "640 Shutter-MaxVolts": "5.0000", "555 AO-MaxVolts": "5.0000", "520 AO-TriggerSequenceLength": "1000", "COM3-BaudRate": "9600", "COM9-BaudRate": "9600", "Vincent-D3-Channel #": "Ch. #1", "PRIME-PMode": "Normal", "OpticalPath-Label": "Left(100)", "BitDepth": 16, "555 AO-TriggerSequenceLength": "1000", "445 Shutter-Description": "NI DAC", "405 AO-Sequenceable": "1", "PRIME-AcquisitionMethod": "Callbacks", "555 AO-Name": "AnalogIO", "PRIME-ShutterMode": "Pre-Exposure", "COM3-Handshaking": "Off", "445 AO-SupportsTriggering": "0", "ROI": "0-0-1200-1200", "COM9-Verbose": "1", "PRIME-Color": "OFF", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "PRIME-ShutterOpenDelay": "0", "COM1-Parity": "None", "Slice": 10, "SlicePosition": 50, "COM9-DelayBetweenCharsMs": "0.0000", "555-Name": "DA Shutter", "PIEZO-LudlSingleAxisName": "B", "XLIGHT Emission Wheel-Label": "725/40 (C)", "640 Shutter-Name": "AnalogIO", "640 AO-Sequenceable": "1", "520 Shutter-Demo": "No", "528 Shutter-Demo": "No", "470-Name": "DA Shutter", "520-DA Device": "520 Shutter", "XYStage-Description": "Ludl XY stage driver adapter", "445 Shutter-MinVolts": "0.0000", "528 AO-TriggerSampleRate": "100000", "405 AO-MinVolts": "0.0000", "405 Shutter-TriggerSequenceLength": "1000", "Height": 1200, "555 AO-Block voltage": "false", "640 Shutter-Volts": "5.0000", "PixelType": "GRAY16", "445 Shutter-Block voltage": "false", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "520 Shutter-TriggerSequenceLength": "1000", "COM9-Name": "COM9", "Core-SLM": "", "555 AO-Volts": "0.0000", "555 Shutter-Block voltage": "false", "XYStage-Speed": "1800.0000", "520 AO-MaxVolts": "5.0000", "528 Shutter-SupportsTriggering": "0", "COM1-Description": "Serial port driver (boost:asio)", "PRIME-MetadataEnabled": "No", "520 Shutter-MinVolts": "0.0000", "470 Shutter-Sequenceable": "1", "PRIME-PreampDelay": "0", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-MinVolts": "0.0000", "ChannelIndex": 3, "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Core-AutoShutter": "1", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "Exposure-ms": 520, "PRIME-PP 2 ENABLED": "Yes", "640 Shutter-TriggerSampleRate": "100000", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "470 Shutter-IOChannel": "Dev1/ao10", "405 AO-Demo": "No", "PixelSizeUm": 1.1, "470 AO-Demo": "No", "470 Shutter-MaxVolts": "5.0000", "Vincent-D3-Name": "Vincent-D3", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FileName": "img_000000000_Il-C_010.tif", "LudlController-Reset": "Operate", "PRIME-Exposure": "520.00", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "445-Name": "DA Shutter", "555 Shutter-Name": "AnalogIO", "520 Shutter-TriggerSampleRate": "100000", "445 Shutter-IOChannel": "Dev1/ao9", "PRIME-TransposeCorrection": "0", "XYStage-StepSize": "0.1000", "445-Description": "DA device that is used as a shutter", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 AO-IOChannel": "Dev1/ao7", "520 Shutter-Name": "AnalogIO", "COM3-DataBits": "8", "640 AO-Volts": "4.5000", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "555 Shutter-IOChannel": "Dev1/ao13", "WaitInterval": null, "520 Shutter-Sequenceable": "1", "YPositionUm": 545.6, "445-State": "0", "445-DA Device": "445 Shutter", "PRIME-CircularBufferAutoSize": "ON", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "555 AO-TriggerSampleRate": "100000", "445 Shutter-Volts": "0.0000", "XLIGHT Hub-Port": "COM3", "Core-AutoFocus": "", "COM4-Handshaking": "Off", "528 Shutter-MinVolts": "0.0000", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "445 Shutter-Name": "AnalogIO", "528 Shutter-Sequenceable": "1", "XYStage-TransposeMirrorX": "0", "XYStage-TransposeMirrorY": "0", "PRIME-ReadoutRate": "100MHz 16bit", "XYStage-Name": "Stage", "640 AO-SupportsTriggering": "0", "640-Name": "DA Shutter", "640 Shutter-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "640 AO-Block voltage": "false", "445 Shutter-Sequenceable": "1", "470 AO-MaxVolts": "5.0000", "COM4-BaudRate": "9600", "470-Description": "DA device that is used as a shutter", "520 Shutter-Description": "NI DAC", "PRIME-CCDTemperature": "-14.8500", "Time": "2019-12-19 09:59:48 -0500", "640 AO-TriggerSequenceLength": "1000", "470 AO-Name": "AnalogIO", "XLIGHT Disk slider-Label": "Disk Pos 40um", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "Binning": "1x1", "TE2000-ModelType": "1", "COM3-AnswerTimeout": "500.0000", "555 Shutter-SupportsTriggering": "0", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "TE2000-Port": "COM4", "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Description": "NI DAC", "COM4-AnswerTimeout": "500.0000", "COM1-DelayBetweenCharsMs": "0.0000", "FilterBlock-Label": "Confocal", "445 AO-Name": "AnalogIO", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-Timing-ReadoutTimeNs": "24672000.0000", "528 AO-Demo": "No", "520 AO-Sequenceable": "1", "555-Description": "DA device that is used as a shutter", "405-DA Device": "405 Shutter", "405 AO-TriggerSequenceLength": "1000", "640 AO-MinVolts": "0.0000", "640 Shutter-Block voltage": "false", "FrameIndex": 0, "PRIME-Port": "CMOS", "555 AO-MinVolts": "0.0000", "COM3-Description": "Serial port driver (boost:asio)", "528 AO-Description": "NI DAC", "555 AO-IOChannel": "Dev1/ao6", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "555 AO-TriggerInputLine": "/Dev1/PFI0", "PRIME-ActualInterval-ms": "390.5200", "LudlController-Port": "COM9", "445 Shutter-Demo": "No", "PRIME-Trigger-Expose Out-Mux": "1", "SliceIndex": 10, "PRIME-CCDTemperatureSetPoint": "-15.0000", "405 Shutter-MinVolts": "0.0000", "PRIME-ExposeOutMode": "First Row", "AxisPositions": null, "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Volts": "0.0000", "528-Name": "DA Shutter", "555-State": "0", "XLIGHT Dichroic-Label": "Di03-R635-t1", "445 AO-Sequenceable": "1", "405-Name": "DA Shutter", "405 AO-IOChannel": "Dev1/ao0", "Core-Camera": "PRIME", "Focus-Name": "Focus", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "405 Shutter-IOChannel": "Dev1/ao8", "640 AO-Demo": "No", "640 Shutter-SupportsTriggering": "0", "445 AO-Demo": "No", "555-DA Device": "555 Shutter", "470 Shutter-Description": "NI DAC", "528 AO-Volts": "0.0000", "COM1-DataBits": "8", "Vincent-D3-Address": "x", "520 AO-SupportsTriggering": "0", "405-Description": "DA device that is used as a shutter", "640 Shutter-MinVolts": "0.0000", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "405 AO-Name": "AnalogIO", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "640 AO-TriggerInputLine": "/Dev1/PFI0", "405 AO-TriggerInputLine": "/Dev1/PFI0", "640-Description": "DA device that is used as a shutter", "445 Shutter-TriggerSampleRate": "100000", "470 Shutter-TriggerSequenceLength": "1000", "COM1-StopBits": "1", "520 Shutter-SupportsTriggering": "0", "405-State": "0", "Core-Initialize": "1", "XLIGHT Emission Wheel-State": "3", "520 AO-Description": "NI DAC", "528 AO-TriggerInputLine": "/Dev1/PFI0", "445 Shutter-MaxVolts": "5.0000", "COM9-Handshaking": "Off", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "XLIGHT Spin motor-Label": "On (spinning)", "PRIME-PP 5 ENABLED": "No", "UUID": "cb95be40-c6b6-47ce-99aa-25c1bc42e9a0", "PRIME-TriggerMode": "Internal Trigger", "COM1-Handshaking": "Off", "PRIME-Timing-PostTriggerDelayNs": "49344000.0000", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "Focus-Position": "17741.5000", "528 Shutter-Block voltage": "false", "Channel": "Il-C", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "555 Shutter-Demo": "No", "405 Shutter-Demo": "No", "528 AO-Name": "AnalogIO", "520-State": "0", "Core-Galvo": "", "405 Shutter-TriggerSampleRate": "100000", "Focus-FocusStepSize_(nm)": "100", "PRIME-PixelType": "16bit", "COM4-DataBits": "8", "470 AO-IOChannel": "Dev1/ao2", "520 AO-MinVolts": "0.0000", "COM9-Parity": "None", "PRIME-CircularBufferEnabled": "ON", "PRIME-PP 6 Reset": "No", "640 AO-Description": "NI DAC", "Source": "PRIME", "445 AO-MinVolts": "0.0000", "PIEZO-Name": "Stage", "445 AO-Description": "NI DAC", "PRIME-CircularBufferFrameCount": "46", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "520 AO-Volts": "0.0000", "528 Shutter-TriggerSampleRate": "100000", "XPositionUm": -1324.9, "OpticalPath-State": "4", "PRIME-PP 3 THRESHOLD": "80", "PRIME-PVCAM Version": "3.7.1", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Gain": "1-HDR", "640 Shutter-TriggerSequenceLength": "1000", "528 AO-SupportsTriggering": "0", "445 AO-TriggerInputLine": "/Dev1/PFI0", "OpticalPath-Name": "OpticalPath", "PRIME-PP 4 ENABLED": "Yes", "PRIME-PVCAM Adapter Version": "1.3.29", "COM3-Name": "COM3", "405 AO-MaxVolts": "5.0000", "520 Shutter-Block voltage": "false", "PRIME-Timing-ClearingTimeNs": "49344000.0000", "Core-Focus": "PIEZO", "528 AO-Block voltage": "false", "TE2000-Description": "Nikon TE2000 hub", "528 AO-MinVolts": "0.0000", "COM4-Parity": "None", "Summary": { "Slices": 11, "ChContrastMin": [ 0, 0, 0, 0, 0, 0 ], "PixelAspect": 1, "SlicesFirst": true, "Interval_ms": 0, "Time": "2019-12-19 09:59:21 -0500", "Prefix": "seq_13", "InitialPositionList": [ { "Label": "Pos0", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.21], "Focus": [17741.5], "XYStage": [ 44542.200000000004, 31636.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos1", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.22], "Focus": [17741.4], "XYStage": [ 43801.100000000006, 31637.9 ] }, "GridColumnIndex": 0 }, { "Label": "Pos2", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.5], "XYStage": [ 38561.3, 32040.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos3", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.185], "Focus": [17741.3], "XYStage": [ 37698.4, 32041.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos4", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.16], "Focus": [17741.5], "XYStage": [ 32375.4, 31758.9 ] }, "GridColumnIndex": 0 }, { "Label": "Pos5", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.17000000000002], "Focus": [17741.3], "XYStage": [ 31770.7, 31758.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos6", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.4], "XYStage": [ 26536.800000000003, 31068.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos7", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.205], "Focus": [17741.4], "XYStage": [ 25420.2, 31068.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos8", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.85], "Focus": [17741.3], "XYStage": [ 21059, 31508 ] }, "GridColumnIndex": 0 }, { "Label": "Pos9", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.82], "Focus": [17741.3], "XYStage": [ 19731.9, 31506.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos10", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.79], "Focus": [17741.4], "XYStage": [ 14528.5, 32007.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos11", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.83], "Focus": [17741.2], "XYStage": [ 13830.300000000001, 32009.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos12", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.79], "Focus": [17741.3], "XYStage": [ 8775.300000000001, 32017.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos13", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.815], "Focus": [17741.3], "XYStage": [ 8109.200000000001, 32008.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos14", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.805], "Focus": [17741.3], "XYStage": [ 3483.3, 31908.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos15", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.82], "Focus": [17741.2], "XYStage": [ 2686.8, 31903.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos16", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [211.48000000000002], "Focus": [17741.1], "XYStage": [ -1978.9, 31973.600000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos17", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [211.51500000000001], "Focus": [17741.1], "XYStage": [ -2957.8, 31982.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos18", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.195], "Focus": [17741.5], "XYStage": [ 47747.4, -576.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos19", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.23000000000002], "Focus": [17741.6], "XYStage": [ 47053.9, -576.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos20", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.205], "Focus": [17741.4], "XYStage": [ 41871.200000000004, -479.8 ] }, "GridColumnIndex": 0 }, { "Label": "Pos21", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.4], "XYStage": [ 40882.600000000006, -392 ] }, "GridColumnIndex": 0 }, { "Label": "Pos22", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.24], "Focus": [17741.4], "XYStage": [ 35905.6, -135.3 ] }, "GridColumnIndex": 0 }, { "Label": "Pos23", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.195], "Focus": [17741.5], "XYStage": [ 34990.9, -132.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos24", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.46], "Focus": [17741.4], "XYStage": [ 29678.100000000002, -958.1 ] }, "GridColumnIndex": 0 }, { "Label": "Pos25", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.44], "Focus": [17741.5], "XYStage": [ 28983.600000000002, -939.4000000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos26", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.475], "Focus": [17741.5], "XYStage": [ 23019.7, -122 ] }, "GridColumnIndex": 0 }, { "Label": "Pos27", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.44], "Focus": [17741.7], "XYStage": [ 22428, -116.80000000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos28", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.46], "Focus": [17741.7], "XYStage": [ 16364.900000000001, -181.10000000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos29", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.48000000000002], "Focus": [17741.5], "XYStage": [ 15712.1, -180 ] }, "GridColumnIndex": 0 }, { "Label": "Pos30", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.45000000000002], "Focus": [17741.7], "XYStage": [ 10346.1, 77.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos31", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.49], "Focus": [17741.4], "XYStage": [ 9755.5, 85.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos32", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.475], "Focus": [17741.5], "XYStage": [ 4775.5, 293.90000000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos33", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.445], "Focus": [17741.4], "XYStage": [ 3954.9, 293.90000000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos34", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.455], "Focus": [17741.4], "XYStage": [ -1324.9, 545.6 ] }, "GridColumnIndex": 0 }, { "Label": "Pos35", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.445], "Focus": [17741.3], "XYStage": [ -2097, 576.6 ] }, "GridColumnIndex": 0 } ], "z-step_um": 10, "GridRow": 0, "BitDepth": 16, "Depth": 2, "Channels": 6, "Source": "Micro-Manager", "ChColors": [ -16711681, -256, -65281, -1, -1, -16776961 ], "MetadataVersion": 10, "KeepShutterOpenChannels": true, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536, 65536 ], "CustomIntervals_ms": [], "Height": 1200, "MicroManagerVersion": "1.4.23 20180219", "GridColumn": 0, "UUID": "18808e7c-f2e6-47b3-90d8-1d779ce87dbd", "PixelType": "GRAY16", "Width": 1200, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\Yan\\20191218-YC113-S7S8-miseq", "Frames": 1, "Comment": "", "UserName": "Nikon", "PixelSize_um": 1.1, "ROI": [ 0, 0, 1200, 1200 ], "Date": "2019-12-19", "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC", "P-DAPI-high" ], "KeepShutterOpenSlices": true, "IJType": 1, "ComputerName": "ZadorConfocal2", "Positions": 36, "PositionIndex": 34, "CameraTimeout": 20000, "TimeFirst": false }, "640 AO-Name": "AnalogIO", "Vincent-D3-Command": "Close" }, "FrameKey-0-4-0": { "COM4-StopBits": "1", "528 AO-MaxVolts": "5.0000", "XLIGHT Excitation Wheel-State": "1", "470 Shutter-Name": "AnalogIO", "Core-Shutter": "Vincent-D3", "555 AO-Demo": "No", "XLIGHT Dichroic-State": "2", "XYStage-StepSize-Y": "0.1000", "ZPositionUm": 168.455, "XYStage-StepSize-X": "0.1000", "PRIME-FirmwareVersion": "20.28", "PRIME-ClearCycles": "2", "520-Name": "DA Shutter", "405 Shutter-Name": "AnalogIO", "PIEZO-StepSize": "0.0050", "PRIME-ClearMode": "Pre-Exposure", "470 AO-MinVolts": "0.0000", "405 AO-SupportsTriggering": "1", "CameraChannelIndex": 0, "XYStage-StartSpeed": "500.0000", "COM9-StopBits": "2", "405 AO-Description": "NI DAC", "COM4-DelayBetweenCharsMs": "0.0000", "445 AO-Block voltage": "false", "520 AO-TriggerInputLine": "/Dev1/PFI0", "555 AO-Description": "NI DAC", "520 AO-TriggerSampleRate": "100000", "COM4-Verbose": "1", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM4-Name": "COM4", "405 Shutter-Volts": "0.0000", "Vincent-D3-Port": "COM1", "PRIME-PP 4 MIN ADU AFFECTED": "200", "470 Shutter-MinVolts": "0.0000", "PRIME-PP 3 ENABLED": "Yes", "470 AO-Description": "NI DAC", "PRIME-Description": "PVCAM API device adapter", "520 Shutter-MaxVolts": "5.0000", "520 Shutter-Volts": "0.0000", "528 AO-Sequenceable": "1", "PRIME-Binning": "1x1", "555 Shutter-MaxVolts": "5.0000", "528 Shutter-TriggerSequenceLength": "1000", "405 Shutter-Block voltage": "false", "XLIGHT Spin motor-State": "1", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "Vincent-D3-Time to open (ms)": "35.0000", "Vincent-D3-Time to close (ms)": "35.0000", "445 AO-TriggerSampleRate": "100000", "640 Shutter-IOChannel": "Dev1/ao14", "555 AO-Sequenceable": "1", "520 AO-Name": "AnalogIO", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "470 AO-Block voltage": "false", "COM1-Verbose": "1", "COM9-AnswerTimeout": "10000.0000", "528 Shutter-Name": "AnalogIO", "PRIME-Trigger Timeout (secs)": "10", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "NextFrame": 0, "470 AO-TriggerSequenceLength": "1000", "PRIME-TransposeXY": "0", "445 AO-TriggerSequenceLength": "1000", "COM1-Name": "COM1", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "COM9-DataBits": "8", "COM1-AnswerTimeout": "500.0000", "Camera": "", "TE2000-Version": "V2.50-V2.50-V2.10", "528 Shutter-Volts": "0.0000", "XLIGHT Hub-Name": "XLIGHT Hub", "PIEZO-Description": "Ludl stage driver adapter", "PositionName": "Pos34", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSampleRate": "100000", "PRIME-FanSpeedSetpoint": "High", "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "405 AO-TriggerSampleRate": "100000", "470 Shutter-Block voltage": "false", "470-State": "0", "COM3-Parity": "None", "405 AO-Volts": "0.0000", "PRIME-TransposeMirrorY": "0", "PRIME-ChipName": "GS144BSI", "470 AO-Volts": "0.0000", "PRIME-TransposeMirrorX": "0", "445 Shutter-SupportsTriggering": "0", "470 Shutter-SupportsTriggering": "0", "XLIGHT Hub-Description": "XLIGHT hub", "PRIME-PreampOffLimit": "10000", "470 AO-Sequenceable": "1", "528 Shutter-MaxVolts": "5.0000", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "Core-XYStage": "XYStage", "470 AO-TriggerSampleRate": "100000", "PRIME-Timing-PreTriggerDelayNs": "24672000.0000", "528-DA Device": "528 Shutter", "Focus-Description": "Nikon TE2000 focus adapter", "FilterBlock-State": "5", "520 AO-Demo": "No", "COM3-DelayBetweenCharsMs": "0.0000", "470 Shutter-Volts": "0.0000", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "PRIME-PP 2 THRESHOLD": "125", "PRIME-PP 4 THRESHOLD": "75", "XYStage-Acceleration": "4.0000", "470 Shutter-Demo": "No", "PRIME-Name": "PMPCIECam00", "640-DA Device": "640 Shutter", "405 AO-Block voltage": "false", "ElapsedTime-ms": 1419063, "PRIME-Offset": "100", "445 AO-IOChannel": "Dev1/ao1", "640 AO-MaxVolts": "5.0000", "PositionIndex": 34, "PRIME-PP 1 ENABLED": "Yes", "COM1-BaudRate": "9600", "640-State": "0", "COM3-Verbose": "1", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "LudlController-TransmissionDelay": "0", "PRIME-FullWellCapacity": "62000", "PRIME-SMARTStreamingEnabled": "No", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "640 Shutter-Description": "NI DAC", "528-Description": "DA device that is used as a shutter", "COM4-Description": "Serial port driver (boost:asio)", "405 Shutter-Description": "NI DAC", "TE2000-Name": "TE2000", "528 Shutter-Description": "NI DAC", "COM9-Description": "Serial port driver (boost:asio)", "LudlController-Name": "LudlController", "445 Shutter-TriggerSequenceLength": "1000", "PRIME-SerialNumber": "A18B203008", "445 AO-Volts": "0.0000", "PRIME-X-dimension": "1200", "PRIME-ShutterCloseDelay": "0", "XLIGHT Disk slider-State": "2", "Position": "Default", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 AO-IOChannel": "Dev1/ao5", "520 AO-IOChannel": "Dev1/ao4", "528 AO-TriggerSequenceLength": "1000", "405 Shutter-MaxVolts": "5.0000", "470-DA Device": "470 Shutter", "PRIME-FTCapable": "No", "Core-ChannelGroup": "Channels", "555 AO-SupportsTriggering": "0", "PRIME-Y-dimension": "1200", "Frame": 0, "555 Shutter-Sequenceable": "1", "PRIME-Timing-ExposureTimeNs": "499998000.0000", "Core-ImageProcessor": "", "528-State": "0", "520 AO-Block voltage": "false", "XLIGHT Excitation Wheel-Label": "Excitation-2", "640 Shutter-Demo": "No", "COM3-StopBits": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "520 Shutter-IOChannel": "Dev1/ao11", "Width": 1200, "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-SupportsTriggering": "0", "640 AO-TriggerSampleRate": "100000", "520-Description": "DA device that is used as a shutter", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "405 Shutter-Sequenceable": "1", "405 Shutter-SupportsTriggering": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-TriggerSampleRate": "100000", "640 Shutter-MaxVolts": "5.0000", "555 AO-MaxVolts": "5.0000", "520 AO-TriggerSequenceLength": "1000", "COM3-BaudRate": "9600", "COM9-BaudRate": "9600", "Vincent-D3-Channel #": "Ch. #1", "PRIME-PMode": "Normal", "OpticalPath-Label": "Left(100)", "BitDepth": 16, "555 AO-TriggerSequenceLength": "1000", "445 Shutter-Description": "NI DAC", "405 AO-Sequenceable": "1", "PRIME-AcquisitionMethod": "Callbacks", "555 AO-Name": "AnalogIO", "PRIME-ShutterMode": "Pre-Exposure", "COM3-Handshaking": "Off", "445 AO-SupportsTriggering": "0", "ROI": "0-0-1200-1200", "COM9-Verbose": "1", "PRIME-Color": "OFF", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "PRIME-ShutterOpenDelay": "0", "COM1-Parity": "None", "Slice": 0, "SlicePosition": -50, "COM9-DelayBetweenCharsMs": "0.0000", "555-Name": "DA Shutter", "PIEZO-LudlSingleAxisName": "B", "XLIGHT Emission Wheel-Label": "Empty", "640 Shutter-Name": "AnalogIO", "640 AO-Sequenceable": "1", "520 Shutter-Demo": "No", "528 Shutter-Demo": "No", "470-Name": "DA Shutter", "520-DA Device": "520 Shutter", "XYStage-Description": "Ludl XY stage driver adapter", "445 Shutter-MinVolts": "0.0000", "528 AO-TriggerSampleRate": "100000", "405 AO-MinVolts": "0.0000", "405 Shutter-TriggerSequenceLength": "1000", "Height": 1200, "555 AO-Block voltage": "false", "640 Shutter-Volts": "0.0000", "PixelType": "GRAY16", "445 Shutter-Block voltage": "false", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "520 Shutter-TriggerSequenceLength": "1000", "COM9-Name": "COM9", "Core-SLM": "", "555 AO-Volts": "0.0000", "555 Shutter-Block voltage": "false", "XYStage-Speed": "1800.0000", "520 AO-MaxVolts": "5.0000", "528 Shutter-SupportsTriggering": "0", "COM1-Description": "Serial port driver (boost:asio)", "PRIME-MetadataEnabled": "No", "520 Shutter-MinVolts": "0.0000", "470 Shutter-Sequenceable": "1", "PRIME-PreampDelay": "0", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-MinVolts": "0.0000", "ChannelIndex": 4, "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Core-AutoShutter": "1", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "Exposure-ms": 20, "PRIME-PP 2 ENABLED": "Yes", "640 Shutter-TriggerSampleRate": "100000", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "470 Shutter-IOChannel": "Dev1/ao10", "405 AO-Demo": "No", "PixelSizeUm": 1.1, "470 AO-Demo": "No", "470 Shutter-MaxVolts": "5.0000", "Vincent-D3-Name": "Vincent-D3", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FileName": "img_000000000_P-DIC_000.tif", "LudlController-Reset": "Operate", "PRIME-Exposure": "20.00", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "445-Name": "DA Shutter", "555 Shutter-Name": "AnalogIO", "520 Shutter-TriggerSampleRate": "100000", "445 Shutter-IOChannel": "Dev1/ao9", "PRIME-TransposeCorrection": "0", "XYStage-StepSize": "0.1000", "445-Description": "DA device that is used as a shutter", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 AO-IOChannel": "Dev1/ao7", "520 Shutter-Name": "AnalogIO", "COM3-DataBits": "8", "640 AO-Volts": "0.0000", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "555 Shutter-IOChannel": "Dev1/ao13", "WaitInterval": null, "520 Shutter-Sequenceable": "1", "YPositionUm": 545.6, "445-State": "0", "445-DA Device": "445 Shutter", "PRIME-CircularBufferAutoSize": "ON", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "555 AO-TriggerSampleRate": "100000", "445 Shutter-Volts": "0.0000", "XLIGHT Hub-Port": "COM3", "Core-AutoFocus": "", "COM4-Handshaking": "Off", "528 Shutter-MinVolts": "0.0000", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "445 Shutter-Name": "AnalogIO", "528 Shutter-Sequenceable": "1", "XYStage-TransposeMirrorX": "0", "XYStage-TransposeMirrorY": "0", "PRIME-ReadoutRate": "100MHz 16bit", "XYStage-Name": "Stage", "640 AO-SupportsTriggering": "0", "640-Name": "DA Shutter", "640 Shutter-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "640 AO-Block voltage": "false", "445 Shutter-Sequenceable": "1", "470 AO-MaxVolts": "5.0000", "COM4-BaudRate": "9600", "470-Description": "DA device that is used as a shutter", "520 Shutter-Description": "NI DAC", "PRIME-CCDTemperature": "-14.8500", "Time": "2019-12-19 09:59:49 -0500", "640 AO-TriggerSequenceLength": "1000", "470 AO-Name": "AnalogIO", "XLIGHT Disk slider-Label": "Disk Pos 40um", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "Binning": "1x1", "TE2000-ModelType": "1", "COM3-AnswerTimeout": "500.0000", "555 Shutter-SupportsTriggering": "0", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "TE2000-Port": "COM4", "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Description": "NI DAC", "COM4-AnswerTimeout": "500.0000", "COM1-DelayBetweenCharsMs": "0.0000", "FilterBlock-Label": "Confocal", "445 AO-Name": "AnalogIO", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-Timing-ReadoutTimeNs": "24672000.0000", "528 AO-Demo": "No", "520 AO-Sequenceable": "1", "555-Description": "DA device that is used as a shutter", "405-DA Device": "405 Shutter", "405 AO-TriggerSequenceLength": "1000", "640 AO-MinVolts": "0.0000", "640 Shutter-Block voltage": "false", "FrameIndex": 0, "PRIME-Port": "CMOS", "555 AO-MinVolts": "0.0000", "COM3-Description": "Serial port driver (boost:asio)", "528 AO-Description": "NI DAC", "555 AO-IOChannel": "Dev1/ao6", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "555 AO-TriggerInputLine": "/Dev1/PFI0", "PRIME-ActualInterval-ms": "390.5200", "LudlController-Port": "COM9", "445 Shutter-Demo": "No", "PRIME-Trigger-Expose Out-Mux": "1", "SliceIndex": 0, "PRIME-CCDTemperatureSetPoint": "-15.0000", "405 Shutter-MinVolts": "0.0000", "PRIME-ExposeOutMode": "First Row", "AxisPositions": null, "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Volts": "0.0000", "528-Name": "DA Shutter", "555-State": "0", "XLIGHT Dichroic-Label": "Di03-R635-t1", "445 AO-Sequenceable": "1", "405-Name": "DA Shutter", "405 AO-IOChannel": "Dev1/ao0", "Core-Camera": "PRIME", "Focus-Name": "Focus", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "405 Shutter-IOChannel": "Dev1/ao8", "640 AO-Demo": "No", "640 Shutter-SupportsTriggering": "0", "445 AO-Demo": "No", "555-DA Device": "555 Shutter", "470 Shutter-Description": "NI DAC", "528 AO-Volts": "0.0000", "COM1-DataBits": "8", "Vincent-D3-Address": "x", "520 AO-SupportsTriggering": "0", "405-Description": "DA device that is used as a shutter", "640 Shutter-MinVolts": "0.0000", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "405 AO-Name": "AnalogIO", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "640 AO-TriggerInputLine": "/Dev1/PFI0", "405 AO-TriggerInputLine": "/Dev1/PFI0", "640-Description": "DA device that is used as a shutter", "445 Shutter-TriggerSampleRate": "100000", "470 Shutter-TriggerSequenceLength": "1000", "COM1-StopBits": "1", "520 Shutter-SupportsTriggering": "0", "405-State": "0", "Core-Initialize": "1", "XLIGHT Emission Wheel-State": "3", "520 AO-Description": "NI DAC", "528 AO-TriggerInputLine": "/Dev1/PFI0", "445 Shutter-MaxVolts": "5.0000", "COM9-Handshaking": "Off", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "XLIGHT Spin motor-Label": "On (spinning)", "PRIME-PP 5 ENABLED": "No", "UUID": "78fc97f4-59ef-44bd-a5bf-94309da0bd33", "PRIME-TriggerMode": "Internal Trigger", "COM1-Handshaking": "Off", "PRIME-Timing-PostTriggerDelayNs": "49344000.0000", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "Focus-Position": "17741.5000", "528 Shutter-Block voltage": "false", "Channel": "P-DIC", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "555 Shutter-Demo": "No", "405 Shutter-Demo": "No", "528 AO-Name": "AnalogIO", "520-State": "0", "Core-Galvo": "", "405 Shutter-TriggerSampleRate": "100000", "Focus-FocusStepSize_(nm)": "100", "PRIME-PixelType": "16bit", "COM4-DataBits": "8", "470 AO-IOChannel": "Dev1/ao2", "520 AO-MinVolts": "0.0000", "COM9-Parity": "None", "PRIME-CircularBufferEnabled": "ON", "PRIME-PP 6 Reset": "No", "640 AO-Description": "NI DAC", "Source": "PRIME", "445 AO-MinVolts": "0.0000", "PIEZO-Name": "Stage", "445 AO-Description": "NI DAC", "PRIME-CircularBufferFrameCount": "46", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "520 AO-Volts": "0.0000", "528 Shutter-TriggerSampleRate": "100000", "XPositionUm": -1324.9, "OpticalPath-State": "4", "PRIME-PP 3 THRESHOLD": "80", "PRIME-PVCAM Version": "3.7.1", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Gain": "1-HDR", "640 Shutter-TriggerSequenceLength": "1000", "528 AO-SupportsTriggering": "0", "445 AO-TriggerInputLine": "/Dev1/PFI0", "OpticalPath-Name": "OpticalPath", "PRIME-PP 4 ENABLED": "Yes", "PRIME-PVCAM Adapter Version": "1.3.29", "COM3-Name": "COM3", "405 AO-MaxVolts": "5.0000", "520 Shutter-Block voltage": "false", "PRIME-Timing-ClearingTimeNs": "49344000.0000", "Core-Focus": "PIEZO", "528 AO-Block voltage": "false", "TE2000-Description": "Nikon TE2000 hub", "528 AO-MinVolts": "0.0000", "COM4-Parity": "None", "Summary": { "Slices": 11, "ChContrastMin": [ 0, 0, 0, 0, 0, 0 ], "PixelAspect": 1, "SlicesFirst": true, "Interval_ms": 0, "Time": "2019-12-19 09:59:21 -0500", "Prefix": "seq_13", "InitialPositionList": [ { "Label": "Pos0", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.21], "Focus": [17741.5], "XYStage": [ 44542.200000000004, 31636.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos1", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.22], "Focus": [17741.4], "XYStage": [ 43801.100000000006, 31637.9 ] }, "GridColumnIndex": 0 }, { "Label": "Pos2", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.5], "XYStage": [ 38561.3, 32040.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos3", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.185], "Focus": [17741.3], "XYStage": [ 37698.4, 32041.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos4", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.16], "Focus": [17741.5], "XYStage": [ 32375.4, 31758.9 ] }, "GridColumnIndex": 0 }, { "Label": "Pos5", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.17000000000002], "Focus": [17741.3], "XYStage": [ 31770.7, 31758.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos6", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.4], "XYStage": [ 26536.800000000003, 31068.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos7", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.205], "Focus": [17741.4], "XYStage": [ 25420.2, 31068.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos8", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.85], "Focus": [17741.3], "XYStage": [ 21059, 31508 ] }, "GridColumnIndex": 0 }, { "Label": "Pos9", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.82], "Focus": [17741.3], "XYStage": [ 19731.9, 31506.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos10", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.79], "Focus": [17741.4], "XYStage": [ 14528.5, 32007.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos11", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.83], "Focus": [17741.2], "XYStage": [ 13830.300000000001, 32009.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos12", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.79], "Focus": [17741.3], "XYStage": [ 8775.300000000001, 32017.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos13", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.815], "Focus": [17741.3], "XYStage": [ 8109.200000000001, 32008.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos14", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.805], "Focus": [17741.3], "XYStage": [ 3483.3, 31908.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos15", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.82], "Focus": [17741.2], "XYStage": [ 2686.8, 31903.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos16", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [211.48000000000002], "Focus": [17741.1], "XYStage": [ -1978.9, 31973.600000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos17", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [211.51500000000001], "Focus": [17741.1], "XYStage": [ -2957.8, 31982.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos18", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.195], "Focus": [17741.5], "XYStage": [ 47747.4, -576.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos19", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.23000000000002], "Focus": [17741.6], "XYStage": [ 47053.9, -576.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos20", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.205], "Focus": [17741.4], "XYStage": [ 41871.200000000004, -479.8 ] }, "GridColumnIndex": 0 }, { "Label": "Pos21", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.4], "XYStage": [ 40882.600000000006, -392 ] }, "GridColumnIndex": 0 }, { "Label": "Pos22", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.24], "Focus": [17741.4], "XYStage": [ 35905.6, -135.3 ] }, "GridColumnIndex": 0 }, { "Label": "Pos23", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.195], "Focus": [17741.5], "XYStage": [ 34990.9, -132.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos24", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.46], "Focus": [17741.4], "XYStage": [ 29678.100000000002, -958.1 ] }, "GridColumnIndex": 0 }, { "Label": "Pos25", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.44], "Focus": [17741.5], "XYStage": [ 28983.600000000002, -939.4000000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos26", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.475], "Focus": [17741.5], "XYStage": [ 23019.7, -122 ] }, "GridColumnIndex": 0 }, { "Label": "Pos27", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.44], "Focus": [17741.7], "XYStage": [ 22428, -116.80000000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos28", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.46], "Focus": [17741.7], "XYStage": [ 16364.900000000001, -181.10000000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos29", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.48000000000002], "Focus": [17741.5], "XYStage": [ 15712.1, -180 ] }, "GridColumnIndex": 0 }, { "Label": "Pos30", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.45000000000002], "Focus": [17741.7], "XYStage": [ 10346.1, 77.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos31", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.49], "Focus": [17741.4], "XYStage": [ 9755.5, 85.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos32", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.475], "Focus": [17741.5], "XYStage": [ 4775.5, 293.90000000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos33", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.445], "Focus": [17741.4], "XYStage": [ 3954.9, 293.90000000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos34", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.455], "Focus": [17741.4], "XYStage": [ -1324.9, 545.6 ] }, "GridColumnIndex": 0 }, { "Label": "Pos35", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.445], "Focus": [17741.3], "XYStage": [ -2097, 576.6 ] }, "GridColumnIndex": 0 } ], "z-step_um": 10, "GridRow": 0, "BitDepth": 16, "Depth": 2, "Channels": 6, "Source": "Micro-Manager", "ChColors": [ -16711681, -256, -65281, -1, -1, -16776961 ], "MetadataVersion": 10, "KeepShutterOpenChannels": true, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536, 65536 ], "CustomIntervals_ms": [], "Height": 1200, "MicroManagerVersion": "1.4.23 20180219", "GridColumn": 0, "UUID": "18808e7c-f2e6-47b3-90d8-1d779ce87dbd", "PixelType": "GRAY16", "Width": 1200, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\Yan\\20191218-YC113-S7S8-miseq", "Frames": 1, "Comment": "", "UserName": "Nikon", "PixelSize_um": 1.1, "ROI": [ 0, 0, 1200, 1200 ], "Date": "2019-12-19", "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC", "P-DAPI-high" ], "KeepShutterOpenSlices": true, "IJType": 1, "ComputerName": "ZadorConfocal2", "Positions": 36, "PositionIndex": 34, "CameraTimeout": 20000, "TimeFirst": false }, "640 AO-Name": "AnalogIO", "Vincent-D3-Command": "Close" }, "FrameKey-0-4-1": { "COM4-StopBits": "1", "528 AO-MaxVolts": "5.0000", "XLIGHT Excitation Wheel-State": "1", "470 Shutter-Name": "AnalogIO", "Core-Shutter": "Vincent-D3", "555 AO-Demo": "No", "XLIGHT Dichroic-State": "2", "XYStage-StepSize-Y": "0.1000", "ZPositionUm": 178.455, "XYStage-StepSize-X": "0.1000", "PRIME-FirmwareVersion": "20.28", "PRIME-ClearCycles": "2", "520-Name": "DA Shutter", "405 Shutter-Name": "AnalogIO", "PIEZO-StepSize": "0.0050", "PRIME-ClearMode": "Pre-Exposure", "470 AO-MinVolts": "0.0000", "405 AO-SupportsTriggering": "1", "CameraChannelIndex": 0, "XYStage-StartSpeed": "500.0000", "COM9-StopBits": "2", "405 AO-Description": "NI DAC", "COM4-DelayBetweenCharsMs": "0.0000", "445 AO-Block voltage": "false", "520 AO-TriggerInputLine": "/Dev1/PFI0", "555 AO-Description": "NI DAC", "520 AO-TriggerSampleRate": "100000", "COM4-Verbose": "1", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM4-Name": "COM4", "405 Shutter-Volts": "0.0000", "Vincent-D3-Port": "COM1", "PRIME-PP 4 MIN ADU AFFECTED": "200", "470 Shutter-MinVolts": "0.0000", "PRIME-PP 3 ENABLED": "Yes", "470 AO-Description": "NI DAC", "PRIME-Description": "PVCAM API device adapter", "520 Shutter-MaxVolts": "5.0000", "520 Shutter-Volts": "0.0000", "528 AO-Sequenceable": "1", "PRIME-Binning": "1x1", "555 Shutter-MaxVolts": "5.0000", "528 Shutter-TriggerSequenceLength": "1000", "405 Shutter-Block voltage": "false", "XLIGHT Spin motor-State": "1", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "Vincent-D3-Time to open (ms)": "35.0000", "Vincent-D3-Time to close (ms)": "35.0000", "445 AO-TriggerSampleRate": "100000", "640 Shutter-IOChannel": "Dev1/ao14", "555 AO-Sequenceable": "1", "520 AO-Name": "AnalogIO", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "470 AO-Block voltage": "false", "COM1-Verbose": "1", "COM9-AnswerTimeout": "10000.0000", "528 Shutter-Name": "AnalogIO", "PRIME-Trigger Timeout (secs)": "10", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "NextFrame": 0, "470 AO-TriggerSequenceLength": "1000", "PRIME-TransposeXY": "0", "445 AO-TriggerSequenceLength": "1000", "COM1-Name": "COM1", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "COM9-DataBits": "8", "COM1-AnswerTimeout": "500.0000", "Camera": "", "TE2000-Version": "V2.50-V2.50-V2.10", "528 Shutter-Volts": "0.0000", "XLIGHT Hub-Name": "XLIGHT Hub", "PIEZO-Description": "Ludl stage driver adapter", "PositionName": "Pos34", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSampleRate": "100000", "PRIME-FanSpeedSetpoint": "High", "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "405 AO-TriggerSampleRate": "100000", "470 Shutter-Block voltage": "false", "470-State": "0", "COM3-Parity": "None", "405 AO-Volts": "0.0000", "PRIME-TransposeMirrorY": "0", "PRIME-ChipName": "GS144BSI", "470 AO-Volts": "0.0000", "PRIME-TransposeMirrorX": "0", "445 Shutter-SupportsTriggering": "0", "470 Shutter-SupportsTriggering": "0", "XLIGHT Hub-Description": "XLIGHT hub", "PRIME-PreampOffLimit": "10000", "470 AO-Sequenceable": "1", "528 Shutter-MaxVolts": "5.0000", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "Core-XYStage": "XYStage", "470 AO-TriggerSampleRate": "100000", "PRIME-Timing-PreTriggerDelayNs": "24672000.0000", "528-DA Device": "528 Shutter", "Focus-Description": "Nikon TE2000 focus adapter", "FilterBlock-State": "5", "520 AO-Demo": "No", "COM3-DelayBetweenCharsMs": "0.0000", "470 Shutter-Volts": "0.0000", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "PRIME-PP 2 THRESHOLD": "125", "PRIME-PP 4 THRESHOLD": "75", "XYStage-Acceleration": "4.0000", "470 Shutter-Demo": "No", "PRIME-Name": "PMPCIECam00", "640-DA Device": "640 Shutter", "405 AO-Block voltage": "false", "ElapsedTime-ms": 1419325, "PRIME-Offset": "100", "445 AO-IOChannel": "Dev1/ao1", "640 AO-MaxVolts": "5.0000", "PositionIndex": 34, "PRIME-PP 1 ENABLED": "Yes", "COM1-BaudRate": "9600", "640-State": "0", "COM3-Verbose": "1", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "LudlController-TransmissionDelay": "0", "PRIME-FullWellCapacity": "62000", "PRIME-SMARTStreamingEnabled": "No", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "640 Shutter-Description": "NI DAC", "528-Description": "DA device that is used as a shutter", "COM4-Description": "Serial port driver (boost:asio)", "405 Shutter-Description": "NI DAC", "TE2000-Name": "TE2000", "528 Shutter-Description": "NI DAC", "COM9-Description": "Serial port driver (boost:asio)", "LudlController-Name": "LudlController", "445 Shutter-TriggerSequenceLength": "1000", "PRIME-SerialNumber": "A18B203008", "445 AO-Volts": "0.0000", "PRIME-X-dimension": "1200", "PRIME-ShutterCloseDelay": "0", "XLIGHT Disk slider-State": "2", "Position": "Default", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 AO-IOChannel": "Dev1/ao5", "520 AO-IOChannel": "Dev1/ao4", "528 AO-TriggerSequenceLength": "1000", "405 Shutter-MaxVolts": "5.0000", "470-DA Device": "470 Shutter", "PRIME-FTCapable": "No", "Core-ChannelGroup": "Channels", "555 AO-SupportsTriggering": "0", "PRIME-Y-dimension": "1200", "Frame": 0, "555 Shutter-Sequenceable": "1", "PRIME-Timing-ExposureTimeNs": "499998000.0000", "Core-ImageProcessor": "", "528-State": "0", "520 AO-Block voltage": "false", "XLIGHT Excitation Wheel-Label": "Excitation-2", "640 Shutter-Demo": "No", "COM3-StopBits": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "520 Shutter-IOChannel": "Dev1/ao11", "Width": 1200, "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-SupportsTriggering": "0", "640 AO-TriggerSampleRate": "100000", "520-Description": "DA device that is used as a shutter", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "405 Shutter-Sequenceable": "1", "405 Shutter-SupportsTriggering": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-TriggerSampleRate": "100000", "640 Shutter-MaxVolts": "5.0000", "555 AO-MaxVolts": "5.0000", "520 AO-TriggerSequenceLength": "1000", "COM3-BaudRate": "9600", "COM9-BaudRate": "9600", "Vincent-D3-Channel #": "Ch. #1", "PRIME-PMode": "Normal", "OpticalPath-Label": "Left(100)", "BitDepth": 16, "555 AO-TriggerSequenceLength": "1000", "445 Shutter-Description": "NI DAC", "405 AO-Sequenceable": "1", "PRIME-AcquisitionMethod": "Callbacks", "555 AO-Name": "AnalogIO", "PRIME-ShutterMode": "Pre-Exposure", "COM3-Handshaking": "Off", "445 AO-SupportsTriggering": "0", "ROI": "0-0-1200-1200", "COM9-Verbose": "1", "PRIME-Color": "OFF", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "PRIME-ShutterOpenDelay": "0", "COM1-Parity": "None", "Slice": 1, "SlicePosition": -40, "COM9-DelayBetweenCharsMs": "0.0000", "555-Name": "DA Shutter", "PIEZO-LudlSingleAxisName": "B", "XLIGHT Emission Wheel-Label": "Empty", "640 Shutter-Name": "AnalogIO", "640 AO-Sequenceable": "1", "520 Shutter-Demo": "No", "528 Shutter-Demo": "No", "470-Name": "DA Shutter", "520-DA Device": "520 Shutter", "XYStage-Description": "Ludl XY stage driver adapter", "445 Shutter-MinVolts": "0.0000", "528 AO-TriggerSampleRate": "100000", "405 AO-MinVolts": "0.0000", "405 Shutter-TriggerSequenceLength": "1000", "Height": 1200, "555 AO-Block voltage": "false", "640 Shutter-Volts": "0.0000", "PixelType": "GRAY16", "445 Shutter-Block voltage": "false", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "520 Shutter-TriggerSequenceLength": "1000", "COM9-Name": "COM9", "Core-SLM": "", "555 AO-Volts": "0.0000", "555 Shutter-Block voltage": "false", "XYStage-Speed": "1800.0000", "520 AO-MaxVolts": "5.0000", "528 Shutter-SupportsTriggering": "0", "COM1-Description": "Serial port driver (boost:asio)", "PRIME-MetadataEnabled": "No", "520 Shutter-MinVolts": "0.0000", "470 Shutter-Sequenceable": "1", "PRIME-PreampDelay": "0", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-MinVolts": "0.0000", "ChannelIndex": 4, "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Core-AutoShutter": "1", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "Exposure-ms": 20, "PRIME-PP 2 ENABLED": "Yes", "640 Shutter-TriggerSampleRate": "100000", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "470 Shutter-IOChannel": "Dev1/ao10", "405 AO-Demo": "No", "PixelSizeUm": 1.1, "470 AO-Demo": "No", "470 Shutter-MaxVolts": "5.0000", "Vincent-D3-Name": "Vincent-D3", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FileName": "img_000000000_P-DIC_001.tif", "LudlController-Reset": "Operate", "PRIME-Exposure": "20.00", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "445-Name": "DA Shutter", "555 Shutter-Name": "AnalogIO", "520 Shutter-TriggerSampleRate": "100000", "445 Shutter-IOChannel": "Dev1/ao9", "PRIME-TransposeCorrection": "0", "XYStage-StepSize": "0.1000", "445-Description": "DA device that is used as a shutter", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 AO-IOChannel": "Dev1/ao7", "520 Shutter-Name": "AnalogIO", "COM3-DataBits": "8", "640 AO-Volts": "0.0000", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "555 Shutter-IOChannel": "Dev1/ao13", "WaitInterval": null, "520 Shutter-Sequenceable": "1", "YPositionUm": 545.6, "445-State": "0", "445-DA Device": "445 Shutter", "PRIME-CircularBufferAutoSize": "ON", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "555 AO-TriggerSampleRate": "100000", "445 Shutter-Volts": "0.0000", "XLIGHT Hub-Port": "COM3", "Core-AutoFocus": "", "COM4-Handshaking": "Off", "528 Shutter-MinVolts": "0.0000", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "445 Shutter-Name": "AnalogIO", "528 Shutter-Sequenceable": "1", "XYStage-TransposeMirrorX": "0", "XYStage-TransposeMirrorY": "0", "PRIME-ReadoutRate": "100MHz 16bit", "XYStage-Name": "Stage", "640 AO-SupportsTriggering": "0", "640-Name": "DA Shutter", "640 Shutter-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "640 AO-Block voltage": "false", "445 Shutter-Sequenceable": "1", "470 AO-MaxVolts": "5.0000", "COM4-BaudRate": "9600", "470-Description": "DA device that is used as a shutter", "520 Shutter-Description": "NI DAC", "PRIME-CCDTemperature": "-14.8500", "Time": "2019-12-19 09:59:49 -0500", "640 AO-TriggerSequenceLength": "1000", "470 AO-Name": "AnalogIO", "XLIGHT Disk slider-Label": "Disk Pos 40um", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "Binning": "1x1", "TE2000-ModelType": "1", "COM3-AnswerTimeout": "500.0000", "555 Shutter-SupportsTriggering": "0", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "TE2000-Port": "COM4", "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Description": "NI DAC", "COM4-AnswerTimeout": "500.0000", "COM1-DelayBetweenCharsMs": "0.0000", "FilterBlock-Label": "Confocal", "445 AO-Name": "AnalogIO", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-Timing-ReadoutTimeNs": "24672000.0000", "528 AO-Demo": "No", "520 AO-Sequenceable": "1", "555-Description": "DA device that is used as a shutter", "405-DA Device": "405 Shutter", "405 AO-TriggerSequenceLength": "1000", "640 AO-MinVolts": "0.0000", "640 Shutter-Block voltage": "false", "FrameIndex": 0, "PRIME-Port": "CMOS", "555 AO-MinVolts": "0.0000", "COM3-Description": "Serial port driver (boost:asio)", "528 AO-Description": "NI DAC", "555 AO-IOChannel": "Dev1/ao6", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "555 AO-TriggerInputLine": "/Dev1/PFI0", "PRIME-ActualInterval-ms": "390.5200", "LudlController-Port": "COM9", "445 Shutter-Demo": "No", "PRIME-Trigger-Expose Out-Mux": "1", "SliceIndex": 1, "PRIME-CCDTemperatureSetPoint": "-15.0000", "405 Shutter-MinVolts": "0.0000", "PRIME-ExposeOutMode": "First Row", "AxisPositions": null, "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Volts": "0.0000", "528-Name": "DA Shutter", "555-State": "0", "XLIGHT Dichroic-Label": "Di03-R635-t1", "445 AO-Sequenceable": "1", "405-Name": "DA Shutter", "405 AO-IOChannel": "Dev1/ao0", "Core-Camera": "PRIME", "Focus-Name": "Focus", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "405 Shutter-IOChannel": "Dev1/ao8", "640 AO-Demo": "No", "640 Shutter-SupportsTriggering": "0", "445 AO-Demo": "No", "555-DA Device": "555 Shutter", "470 Shutter-Description": "NI DAC", "528 AO-Volts": "0.0000", "COM1-DataBits": "8", "Vincent-D3-Address": "x", "520 AO-SupportsTriggering": "0", "405-Description": "DA device that is used as a shutter", "640 Shutter-MinVolts": "0.0000", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "405 AO-Name": "AnalogIO", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "640 AO-TriggerInputLine": "/Dev1/PFI0", "405 AO-TriggerInputLine": "/Dev1/PFI0", "640-Description": "DA device that is used as a shutter", "445 Shutter-TriggerSampleRate": "100000", "470 Shutter-TriggerSequenceLength": "1000", "COM1-StopBits": "1", "520 Shutter-SupportsTriggering": "0", "405-State": "0", "Core-Initialize": "1", "XLIGHT Emission Wheel-State": "3", "520 AO-Description": "NI DAC", "528 AO-TriggerInputLine": "/Dev1/PFI0", "445 Shutter-MaxVolts": "5.0000", "COM9-Handshaking": "Off", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "XLIGHT Spin motor-Label": "On (spinning)", "PRIME-PP 5 ENABLED": "No", "UUID": "44ff4a93-f7fd-44e0-9bef-c46f826caa4f", "PRIME-TriggerMode": "Internal Trigger", "COM1-Handshaking": "Off", "PRIME-Timing-PostTriggerDelayNs": "49344000.0000", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "Focus-Position": "17741.5000", "528 Shutter-Block voltage": "false", "Channel": "P-DIC", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "555 Shutter-Demo": "No", "405 Shutter-Demo": "No", "528 AO-Name": "AnalogIO", "520-State": "0", "Core-Galvo": "", "405 Shutter-TriggerSampleRate": "100000", "Focus-FocusStepSize_(nm)": "100", "PRIME-PixelType": "16bit", "COM4-DataBits": "8", "470 AO-IOChannel": "Dev1/ao2", "520 AO-MinVolts": "0.0000", "COM9-Parity": "None", "PRIME-CircularBufferEnabled": "ON", "PRIME-PP 6 Reset": "No", "640 AO-Description": "NI DAC", "Source": "PRIME", "445 AO-MinVolts": "0.0000", "PIEZO-Name": "Stage", "445 AO-Description": "NI DAC", "PRIME-CircularBufferFrameCount": "46", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "520 AO-Volts": "0.0000", "528 Shutter-TriggerSampleRate": "100000", "XPositionUm": -1324.9, "OpticalPath-State": "4", "PRIME-PP 3 THRESHOLD": "80", "PRIME-PVCAM Version": "3.7.1", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Gain": "1-HDR", "640 Shutter-TriggerSequenceLength": "1000", "528 AO-SupportsTriggering": "0", "445 AO-TriggerInputLine": "/Dev1/PFI0", "OpticalPath-Name": "OpticalPath", "PRIME-PP 4 ENABLED": "Yes", "PRIME-PVCAM Adapter Version": "1.3.29", "COM3-Name": "COM3", "405 AO-MaxVolts": "5.0000", "520 Shutter-Block voltage": "false", "PRIME-Timing-ClearingTimeNs": "49344000.0000", "Core-Focus": "PIEZO", "528 AO-Block voltage": "false", "TE2000-Description": "Nikon TE2000 hub", "528 AO-MinVolts": "0.0000", "COM4-Parity": "None", "Summary": { "Slices": 11, "ChContrastMin": [ 0, 0, 0, 0, 0, 0 ], "PixelAspect": 1, "SlicesFirst": true, "Interval_ms": 0, "Time": "2019-12-19 09:59:21 -0500", "Prefix": "seq_13", "InitialPositionList": [ { "Label": "Pos0", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.21], "Focus": [17741.5], "XYStage": [ 44542.200000000004, 31636.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos1", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.22], "Focus": [17741.4], "XYStage": [ 43801.100000000006, 31637.9 ] }, "GridColumnIndex": 0 }, { "Label": "Pos2", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.5], "XYStage": [ 38561.3, 32040.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos3", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.185], "Focus": [17741.3], "XYStage": [ 37698.4, 32041.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos4", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.16], "Focus": [17741.5], "XYStage": [ 32375.4, 31758.9 ] }, "GridColumnIndex": 0 }, { "Label": "Pos5", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.17000000000002], "Focus": [17741.3], "XYStage": [ 31770.7, 31758.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos6", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.4], "XYStage": [ 26536.800000000003, 31068.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos7", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.205], "Focus": [17741.4], "XYStage": [ 25420.2, 31068.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos8", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.85], "Focus": [17741.3], "XYStage": [ 21059, 31508 ] }, "GridColumnIndex": 0 }, { "Label": "Pos9", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.82], "Focus": [17741.3], "XYStage": [ 19731.9, 31506.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos10", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.79], "Focus": [17741.4], "XYStage": [ 14528.5, 32007.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos11", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.83], "Focus": [17741.2], "XYStage": [ 13830.300000000001, 32009.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos12", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.79], "Focus": [17741.3], "XYStage": [ 8775.300000000001, 32017.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos13", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.815], "Focus": [17741.3], "XYStage": [ 8109.200000000001, 32008.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos14", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.805], "Focus": [17741.3], "XYStage": [ 3483.3, 31908.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos15", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.82], "Focus": [17741.2], "XYStage": [ 2686.8, 31903.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos16", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [211.48000000000002], "Focus": [17741.1], "XYStage": [ -1978.9, 31973.600000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos17", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [211.51500000000001], "Focus": [17741.1], "XYStage": [ -2957.8, 31982.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos18", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.195], "Focus": [17741.5], "XYStage": [ 47747.4, -576.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos19", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.23000000000002], "Focus": [17741.6], "XYStage": [ 47053.9, -576.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos20", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.205], "Focus": [17741.4], "XYStage": [ 41871.200000000004, -479.8 ] }, "GridColumnIndex": 0 }, { "Label": "Pos21", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.4], "XYStage": [ 40882.600000000006, -392 ] }, "GridColumnIndex": 0 }, { "Label": "Pos22", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.24], "Focus": [17741.4], "XYStage": [ 35905.6, -135.3 ] }, "GridColumnIndex": 0 }, { "Label": "Pos23", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.195], "Focus": [17741.5], "XYStage": [ 34990.9, -132.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos24", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.46], "Focus": [17741.4], "XYStage": [ 29678.100000000002, -958.1 ] }, "GridColumnIndex": 0 }, { "Label": "Pos25", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.44], "Focus": [17741.5], "XYStage": [ 28983.600000000002, -939.4000000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos26", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.475], "Focus": [17741.5], "XYStage": [ 23019.7, -122 ] }, "GridColumnIndex": 0 }, { "Label": "Pos27", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.44], "Focus": [17741.7], "XYStage": [ 22428, -116.80000000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos28", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.46], "Focus": [17741.7], "XYStage": [ 16364.900000000001, -181.10000000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos29", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.48000000000002], "Focus": [17741.5], "XYStage": [ 15712.1, -180 ] }, "GridColumnIndex": 0 }, { "Label": "Pos30", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.45000000000002], "Focus": [17741.7], "XYStage": [ 10346.1, 77.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos31", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.49], "Focus": [17741.4], "XYStage": [ 9755.5, 85.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos32", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.475], "Focus": [17741.5], "XYStage": [ 4775.5, 293.90000000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos33", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.445], "Focus": [17741.4], "XYStage": [ 3954.9, 293.90000000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos34", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.455], "Focus": [17741.4], "XYStage": [ -1324.9, 545.6 ] }, "GridColumnIndex": 0 }, { "Label": "Pos35", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.445], "Focus": [17741.3], "XYStage": [ -2097, 576.6 ] }, "GridColumnIndex": 0 } ], "z-step_um": 10, "GridRow": 0, "BitDepth": 16, "Depth": 2, "Channels": 6, "Source": "Micro-Manager", "ChColors": [ -16711681, -256, -65281, -1, -1, -16776961 ], "MetadataVersion": 10, "KeepShutterOpenChannels": true, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536, 65536 ], "CustomIntervals_ms": [], "Height": 1200, "MicroManagerVersion": "1.4.23 20180219", "GridColumn": 0, "UUID": "18808e7c-f2e6-47b3-90d8-1d779ce87dbd", "PixelType": "GRAY16", "Width": 1200, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\Yan\\20191218-YC113-S7S8-miseq", "Frames": 1, "Comment": "", "UserName": "Nikon", "PixelSize_um": 1.1, "ROI": [ 0, 0, 1200, 1200 ], "Date": "2019-12-19", "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC", "P-DAPI-high" ], "KeepShutterOpenSlices": true, "IJType": 1, "ComputerName": "ZadorConfocal2", "Positions": 36, "PositionIndex": 34, "CameraTimeout": 20000, "TimeFirst": false }, "640 AO-Name": "AnalogIO", "Vincent-D3-Command": "Close" }, "FrameKey-0-4-2": { "COM4-StopBits": "1", "528 AO-MaxVolts": "5.0000", "XLIGHT Excitation Wheel-State": "1", "470 Shutter-Name": "AnalogIO", "Core-Shutter": "Vincent-D3", "555 AO-Demo": "No", "XLIGHT Dichroic-State": "2", "XYStage-StepSize-Y": "0.1000", "ZPositionUm": 188.455, "XYStage-StepSize-X": "0.1000", "PRIME-FirmwareVersion": "20.28", "PRIME-ClearCycles": "2", "520-Name": "DA Shutter", "405 Shutter-Name": "AnalogIO", "PIEZO-StepSize": "0.0050", "PRIME-ClearMode": "Pre-Exposure", "470 AO-MinVolts": "0.0000", "405 AO-SupportsTriggering": "1", "CameraChannelIndex": 0, "XYStage-StartSpeed": "500.0000", "COM9-StopBits": "2", "405 AO-Description": "NI DAC", "COM4-DelayBetweenCharsMs": "0.0000", "445 AO-Block voltage": "false", "520 AO-TriggerInputLine": "/Dev1/PFI0", "555 AO-Description": "NI DAC", "520 AO-TriggerSampleRate": "100000", "COM4-Verbose": "1", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM4-Name": "COM4", "405 Shutter-Volts": "0.0000", "Vincent-D3-Port": "COM1", "PRIME-PP 4 MIN ADU AFFECTED": "200", "470 Shutter-MinVolts": "0.0000", "PRIME-PP 3 ENABLED": "Yes", "470 AO-Description": "NI DAC", "PRIME-Description": "PVCAM API device adapter", "520 Shutter-MaxVolts": "5.0000", "520 Shutter-Volts": "0.0000", "528 AO-Sequenceable": "1", "PRIME-Binning": "1x1", "555 Shutter-MaxVolts": "5.0000", "528 Shutter-TriggerSequenceLength": "1000", "405 Shutter-Block voltage": "false", "XLIGHT Spin motor-State": "1", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "Vincent-D3-Time to open (ms)": "35.0000", "Vincent-D3-Time to close (ms)": "35.0000", "445 AO-TriggerSampleRate": "100000", "640 Shutter-IOChannel": "Dev1/ao14", "555 AO-Sequenceable": "1", "520 AO-Name": "AnalogIO", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "470 AO-Block voltage": "false", "COM1-Verbose": "1", "COM9-AnswerTimeout": "10000.0000", "528 Shutter-Name": "AnalogIO", "PRIME-Trigger Timeout (secs)": "10", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "NextFrame": 0, "470 AO-TriggerSequenceLength": "1000", "PRIME-TransposeXY": "0", "445 AO-TriggerSequenceLength": "1000", "COM1-Name": "COM1", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "COM9-DataBits": "8", "COM1-AnswerTimeout": "500.0000", "Camera": "", "TE2000-Version": "V2.50-V2.50-V2.10", "528 Shutter-Volts": "0.0000", "XLIGHT Hub-Name": "XLIGHT Hub", "PIEZO-Description": "Ludl stage driver adapter", "PositionName": "Pos34", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSampleRate": "100000", "PRIME-FanSpeedSetpoint": "High", "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "405 AO-TriggerSampleRate": "100000", "470 Shutter-Block voltage": "false", "470-State": "0", "COM3-Parity": "None", "405 AO-Volts": "0.0000", "PRIME-TransposeMirrorY": "0", "PRIME-ChipName": "GS144BSI", "470 AO-Volts": "0.0000", "PRIME-TransposeMirrorX": "0", "445 Shutter-SupportsTriggering": "0", "470 Shutter-SupportsTriggering": "0", "XLIGHT Hub-Description": "XLIGHT hub", "PRIME-PreampOffLimit": "10000", "470 AO-Sequenceable": "1", "528 Shutter-MaxVolts": "5.0000", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "Core-XYStage": "XYStage", "470 AO-TriggerSampleRate": "100000", "PRIME-Timing-PreTriggerDelayNs": "24672000.0000", "528-DA Device": "528 Shutter", "Focus-Description": "Nikon TE2000 focus adapter", "FilterBlock-State": "5", "520 AO-Demo": "No", "COM3-DelayBetweenCharsMs": "0.0000", "470 Shutter-Volts": "0.0000", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "PRIME-PP 2 THRESHOLD": "125", "PRIME-PP 4 THRESHOLD": "75", "XYStage-Acceleration": "4.0000", "470 Shutter-Demo": "No", "PRIME-Name": "PMPCIECam00", "640-DA Device": "640 Shutter", "405 AO-Block voltage": "false", "ElapsedTime-ms": 1419579, "PRIME-Offset": "100", "445 AO-IOChannel": "Dev1/ao1", "640 AO-MaxVolts": "5.0000", "PositionIndex": 34, "PRIME-PP 1 ENABLED": "Yes", "COM1-BaudRate": "9600", "640-State": "0", "COM3-Verbose": "1", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "LudlController-TransmissionDelay": "0", "PRIME-FullWellCapacity": "62000", "PRIME-SMARTStreamingEnabled": "No", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "640 Shutter-Description": "NI DAC", "528-Description": "DA device that is used as a shutter", "COM4-Description": "Serial port driver (boost:asio)", "405 Shutter-Description": "NI DAC", "TE2000-Name": "TE2000", "528 Shutter-Description": "NI DAC", "COM9-Description": "Serial port driver (boost:asio)", "LudlController-Name": "LudlController", "445 Shutter-TriggerSequenceLength": "1000", "PRIME-SerialNumber": "A18B203008", "445 AO-Volts": "0.0000", "PRIME-X-dimension": "1200", "PRIME-ShutterCloseDelay": "0", "XLIGHT Disk slider-State": "2", "Position": "Default", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 AO-IOChannel": "Dev1/ao5", "520 AO-IOChannel": "Dev1/ao4", "528 AO-TriggerSequenceLength": "1000", "405 Shutter-MaxVolts": "5.0000", "470-DA Device": "470 Shutter", "PRIME-FTCapable": "No", "Core-ChannelGroup": "Channels", "555 AO-SupportsTriggering": "0", "PRIME-Y-dimension": "1200", "Frame": 0, "555 Shutter-Sequenceable": "1", "PRIME-Timing-ExposureTimeNs": "499998000.0000", "Core-ImageProcessor": "", "528-State": "0", "520 AO-Block voltage": "false", "XLIGHT Excitation Wheel-Label": "Excitation-2", "640 Shutter-Demo": "No", "COM3-StopBits": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "520 Shutter-IOChannel": "Dev1/ao11", "Width": 1200, "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-SupportsTriggering": "0", "640 AO-TriggerSampleRate": "100000", "520-Description": "DA device that is used as a shutter", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "405 Shutter-Sequenceable": "1", "405 Shutter-SupportsTriggering": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-TriggerSampleRate": "100000", "640 Shutter-MaxVolts": "5.0000", "555 AO-MaxVolts": "5.0000", "520 AO-TriggerSequenceLength": "1000", "COM3-BaudRate": "9600", "COM9-BaudRate": "9600", "Vincent-D3-Channel #": "Ch. #1", "PRIME-PMode": "Normal", "OpticalPath-Label": "Left(100)", "BitDepth": 16, "555 AO-TriggerSequenceLength": "1000", "445 Shutter-Description": "NI DAC", "405 AO-Sequenceable": "1", "PRIME-AcquisitionMethod": "Callbacks", "555 AO-Name": "AnalogIO", "PRIME-ShutterMode": "Pre-Exposure", "COM3-Handshaking": "Off", "445 AO-SupportsTriggering": "0", "ROI": "0-0-1200-1200", "COM9-Verbose": "1", "PRIME-Color": "OFF", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "PRIME-ShutterOpenDelay": "0", "COM1-Parity": "None", "Slice": 2, "SlicePosition": -30, "COM9-DelayBetweenCharsMs": "0.0000", "555-Name": "DA Shutter", "PIEZO-LudlSingleAxisName": "B", "XLIGHT Emission Wheel-Label": "Empty", "640 Shutter-Name": "AnalogIO", "640 AO-Sequenceable": "1", "520 Shutter-Demo": "No", "528 Shutter-Demo": "No", "470-Name": "DA Shutter", "520-DA Device": "520 Shutter", "XYStage-Description": "Ludl XY stage driver adapter", "445 Shutter-MinVolts": "0.0000", "528 AO-TriggerSampleRate": "100000", "405 AO-MinVolts": "0.0000", "405 Shutter-TriggerSequenceLength": "1000", "Height": 1200, "555 AO-Block voltage": "false", "640 Shutter-Volts": "0.0000", "PixelType": "GRAY16", "445 Shutter-Block voltage": "false", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "520 Shutter-TriggerSequenceLength": "1000", "COM9-Name": "COM9", "Core-SLM": "", "555 AO-Volts": "0.0000", "555 Shutter-Block voltage": "false", "XYStage-Speed": "1800.0000", "520 AO-MaxVolts": "5.0000", "528 Shutter-SupportsTriggering": "0", "COM1-Description": "Serial port driver (boost:asio)", "PRIME-MetadataEnabled": "No", "520 Shutter-MinVolts": "0.0000", "470 Shutter-Sequenceable": "1", "PRIME-PreampDelay": "0", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-MinVolts": "0.0000", "ChannelIndex": 4, "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Core-AutoShutter": "1", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "Exposure-ms": 20, "PRIME-PP 2 ENABLED": "Yes", "640 Shutter-TriggerSampleRate": "100000", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "470 Shutter-IOChannel": "Dev1/ao10", "405 AO-Demo": "No", "PixelSizeUm": 1.1, "470 AO-Demo": "No", "470 Shutter-MaxVolts": "5.0000", "Vincent-D3-Name": "Vincent-D3", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FileName": "img_000000000_P-DIC_002.tif", "LudlController-Reset": "Operate", "PRIME-Exposure": "20.00", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "445-Name": "DA Shutter", "555 Shutter-Name": "AnalogIO", "520 Shutter-TriggerSampleRate": "100000", "445 Shutter-IOChannel": "Dev1/ao9", "PRIME-TransposeCorrection": "0", "XYStage-StepSize": "0.1000", "445-Description": "DA device that is used as a shutter", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 AO-IOChannel": "Dev1/ao7", "520 Shutter-Name": "AnalogIO", "COM3-DataBits": "8", "640 AO-Volts": "0.0000", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "555 Shutter-IOChannel": "Dev1/ao13", "WaitInterval": null, "520 Shutter-Sequenceable": "1", "YPositionUm": 545.6, "445-State": "0", "445-DA Device": "445 Shutter", "PRIME-CircularBufferAutoSize": "ON", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "555 AO-TriggerSampleRate": "100000", "445 Shutter-Volts": "0.0000", "XLIGHT Hub-Port": "COM3", "Core-AutoFocus": "", "COM4-Handshaking": "Off", "528 Shutter-MinVolts": "0.0000", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "445 Shutter-Name": "AnalogIO", "528 Shutter-Sequenceable": "1", "XYStage-TransposeMirrorX": "0", "XYStage-TransposeMirrorY": "0", "PRIME-ReadoutRate": "100MHz 16bit", "XYStage-Name": "Stage", "640 AO-SupportsTriggering": "0", "640-Name": "DA Shutter", "640 Shutter-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "640 AO-Block voltage": "false", "445 Shutter-Sequenceable": "1", "470 AO-MaxVolts": "5.0000", "COM4-BaudRate": "9600", "470-Description": "DA device that is used as a shutter", "520 Shutter-Description": "NI DAC", "PRIME-CCDTemperature": "-14.8500", "Time": "2019-12-19 09:59:49 -0500", "640 AO-TriggerSequenceLength": "1000", "470 AO-Name": "AnalogIO", "XLIGHT Disk slider-Label": "Disk Pos 40um", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "Binning": "1x1", "TE2000-ModelType": "1", "COM3-AnswerTimeout": "500.0000", "555 Shutter-SupportsTriggering": "0", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "TE2000-Port": "COM4", "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Description": "NI DAC", "COM4-AnswerTimeout": "500.0000", "COM1-DelayBetweenCharsMs": "0.0000", "FilterBlock-Label": "Confocal", "445 AO-Name": "AnalogIO", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-Timing-ReadoutTimeNs": "24672000.0000", "528 AO-Demo": "No", "520 AO-Sequenceable": "1", "555-Description": "DA device that is used as a shutter", "405-DA Device": "405 Shutter", "405 AO-TriggerSequenceLength": "1000", "640 AO-MinVolts": "0.0000", "640 Shutter-Block voltage": "false", "FrameIndex": 0, "PRIME-Port": "CMOS", "555 AO-MinVolts": "0.0000", "COM3-Description": "Serial port driver (boost:asio)", "528 AO-Description": "NI DAC", "555 AO-IOChannel": "Dev1/ao6", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "555 AO-TriggerInputLine": "/Dev1/PFI0", "PRIME-ActualInterval-ms": "390.5200", "LudlController-Port": "COM9", "445 Shutter-Demo": "No", "PRIME-Trigger-Expose Out-Mux": "1", "SliceIndex": 2, "PRIME-CCDTemperatureSetPoint": "-15.0000", "405 Shutter-MinVolts": "0.0000", "PRIME-ExposeOutMode": "First Row", "AxisPositions": null, "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Volts": "0.0000", "528-Name": "DA Shutter", "555-State": "0", "XLIGHT Dichroic-Label": "Di03-R635-t1", "445 AO-Sequenceable": "1", "405-Name": "DA Shutter", "405 AO-IOChannel": "Dev1/ao0", "Core-Camera": "PRIME", "Focus-Name": "Focus", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "405 Shutter-IOChannel": "Dev1/ao8", "640 AO-Demo": "No", "640 Shutter-SupportsTriggering": "0", "445 AO-Demo": "No", "555-DA Device": "555 Shutter", "470 Shutter-Description": "NI DAC", "528 AO-Volts": "0.0000", "COM1-DataBits": "8", "Vincent-D3-Address": "x", "520 AO-SupportsTriggering": "0", "405-Description": "DA device that is used as a shutter", "640 Shutter-MinVolts": "0.0000", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "405 AO-Name": "AnalogIO", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "640 AO-TriggerInputLine": "/Dev1/PFI0", "405 AO-TriggerInputLine": "/Dev1/PFI0", "640-Description": "DA device that is used as a shutter", "445 Shutter-TriggerSampleRate": "100000", "470 Shutter-TriggerSequenceLength": "1000", "COM1-StopBits": "1", "520 Shutter-SupportsTriggering": "0", "405-State": "0", "Core-Initialize": "1", "XLIGHT Emission Wheel-State": "3", "520 AO-Description": "NI DAC", "528 AO-TriggerInputLine": "/Dev1/PFI0", "445 Shutter-MaxVolts": "5.0000", "COM9-Handshaking": "Off", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "XLIGHT Spin motor-Label": "On (spinning)", "PRIME-PP 5 ENABLED": "No", "UUID": "a167d4d4-8a4a-4b25-a08a-bd10b67662f1", "PRIME-TriggerMode": "Internal Trigger", "COM1-Handshaking": "Off", "PRIME-Timing-PostTriggerDelayNs": "49344000.0000", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "Focus-Position": "17741.5000", "528 Shutter-Block voltage": "false", "Channel": "P-DIC", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "555 Shutter-Demo": "No", "405 Shutter-Demo": "No", "528 AO-Name": "AnalogIO", "520-State": "0", "Core-Galvo": "", "405 Shutter-TriggerSampleRate": "100000", "Focus-FocusStepSize_(nm)": "100", "PRIME-PixelType": "16bit", "COM4-DataBits": "8", "470 AO-IOChannel": "Dev1/ao2", "520 AO-MinVolts": "0.0000", "COM9-Parity": "None", "PRIME-CircularBufferEnabled": "ON", "PRIME-PP 6 Reset": "No", "640 AO-Description": "NI DAC", "Source": "PRIME", "445 AO-MinVolts": "0.0000", "PIEZO-Name": "Stage", "445 AO-Description": "NI DAC", "PRIME-CircularBufferFrameCount": "46", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "520 AO-Volts": "0.0000", "528 Shutter-TriggerSampleRate": "100000", "XPositionUm": -1324.9, "OpticalPath-State": "4", "PRIME-PP 3 THRESHOLD": "80", "PRIME-PVCAM Version": "3.7.1", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Gain": "1-HDR", "640 Shutter-TriggerSequenceLength": "1000", "528 AO-SupportsTriggering": "0", "445 AO-TriggerInputLine": "/Dev1/PFI0", "OpticalPath-Name": "OpticalPath", "PRIME-PP 4 ENABLED": "Yes", "PRIME-PVCAM Adapter Version": "1.3.29", "COM3-Name": "COM3", "405 AO-MaxVolts": "5.0000", "520 Shutter-Block voltage": "false", "PRIME-Timing-ClearingTimeNs": "49344000.0000", "Core-Focus": "PIEZO", "528 AO-Block voltage": "false", "TE2000-Description": "Nikon TE2000 hub", "528 AO-MinVolts": "0.0000", "COM4-Parity": "None", "Summary": { "Slices": 11, "ChContrastMin": [ 0, 0, 0, 0, 0, 0 ], "PixelAspect": 1, "SlicesFirst": true, "Interval_ms": 0, "Time": "2019-12-19 09:59:21 -0500", "Prefix": "seq_13", "InitialPositionList": [ { "Label": "Pos0", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.21], "Focus": [17741.5], "XYStage": [ 44542.200000000004, 31636.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos1", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.22], "Focus": [17741.4], "XYStage": [ 43801.100000000006, 31637.9 ] }, "GridColumnIndex": 0 }, { "Label": "Pos2", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.5], "XYStage": [ 38561.3, 32040.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos3", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.185], "Focus": [17741.3], "XYStage": [ 37698.4, 32041.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos4", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.16], "Focus": [17741.5], "XYStage": [ 32375.4, 31758.9 ] }, "GridColumnIndex": 0 }, { "Label": "Pos5", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.17000000000002], "Focus": [17741.3], "XYStage": [ 31770.7, 31758.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos6", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.4], "XYStage": [ 26536.800000000003, 31068.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos7", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.205], "Focus": [17741.4], "XYStage": [ 25420.2, 31068.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos8", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.85], "Focus": [17741.3], "XYStage": [ 21059, 31508 ] }, "GridColumnIndex": 0 }, { "Label": "Pos9", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.82], "Focus": [17741.3], "XYStage": [ 19731.9, 31506.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos10", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.79], "Focus": [17741.4], "XYStage": [ 14528.5, 32007.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos11", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.83], "Focus": [17741.2], "XYStage": [ 13830.300000000001, 32009.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos12", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.79], "Focus": [17741.3], "XYStage": [ 8775.300000000001, 32017.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos13", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.815], "Focus": [17741.3], "XYStage": [ 8109.200000000001, 32008.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos14", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.805], "Focus": [17741.3], "XYStage": [ 3483.3, 31908.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos15", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.82], "Focus": [17741.2], "XYStage": [ 2686.8, 31903.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos16", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [211.48000000000002], "Focus": [17741.1], "XYStage": [ -1978.9, 31973.600000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos17", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [211.51500000000001], "Focus": [17741.1], "XYStage": [ -2957.8, 31982.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos18", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.195], "Focus": [17741.5], "XYStage": [ 47747.4, -576.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos19", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.23000000000002], "Focus": [17741.6], "XYStage": [ 47053.9, -576.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos20", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.205], "Focus": [17741.4], "XYStage": [ 41871.200000000004, -479.8 ] }, "GridColumnIndex": 0 }, { "Label": "Pos21", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.4], "XYStage": [ 40882.600000000006, -392 ] }, "GridColumnIndex": 0 }, { "Label": "Pos22", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.24], "Focus": [17741.4], "XYStage": [ 35905.6, -135.3 ] }, "GridColumnIndex": 0 }, { "Label": "Pos23", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.195], "Focus": [17741.5], "XYStage": [ 34990.9, -132.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos24", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.46], "Focus": [17741.4], "XYStage": [ 29678.100000000002, -958.1 ] }, "GridColumnIndex": 0 }, { "Label": "Pos25", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.44], "Focus": [17741.5], "XYStage": [ 28983.600000000002, -939.4000000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos26", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.475], "Focus": [17741.5], "XYStage": [ 23019.7, -122 ] }, "GridColumnIndex": 0 }, { "Label": "Pos27", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.44], "Focus": [17741.7], "XYStage": [ 22428, -116.80000000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos28", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.46], "Focus": [17741.7], "XYStage": [ 16364.900000000001, -181.10000000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos29", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.48000000000002], "Focus": [17741.5], "XYStage": [ 15712.1, -180 ] }, "GridColumnIndex": 0 }, { "Label": "Pos30", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.45000000000002], "Focus": [17741.7], "XYStage": [ 10346.1, 77.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos31", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.49], "Focus": [17741.4], "XYStage": [ 9755.5, 85.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos32", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.475], "Focus": [17741.5], "XYStage": [ 4775.5, 293.90000000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos33", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.445], "Focus": [17741.4], "XYStage": [ 3954.9, 293.90000000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos34", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.455], "Focus": [17741.4], "XYStage": [ -1324.9, 545.6 ] }, "GridColumnIndex": 0 }, { "Label": "Pos35", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.445], "Focus": [17741.3], "XYStage": [ -2097, 576.6 ] }, "GridColumnIndex": 0 } ], "z-step_um": 10, "GridRow": 0, "BitDepth": 16, "Depth": 2, "Channels": 6, "Source": "Micro-Manager", "ChColors": [ -16711681, -256, -65281, -1, -1, -16776961 ], "MetadataVersion": 10, "KeepShutterOpenChannels": true, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536, 65536 ], "CustomIntervals_ms": [], "Height": 1200, "MicroManagerVersion": "1.4.23 20180219", "GridColumn": 0, "UUID": "18808e7c-f2e6-47b3-90d8-1d779ce87dbd", "PixelType": "GRAY16", "Width": 1200, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\Yan\\20191218-YC113-S7S8-miseq", "Frames": 1, "Comment": "", "UserName": "Nikon", "PixelSize_um": 1.1, "ROI": [ 0, 0, 1200, 1200 ], "Date": "2019-12-19", "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC", "P-DAPI-high" ], "KeepShutterOpenSlices": true, "IJType": 1, "ComputerName": "ZadorConfocal2", "Positions": 36, "PositionIndex": 34, "CameraTimeout": 20000, "TimeFirst": false }, "640 AO-Name": "AnalogIO", "Vincent-D3-Command": "Close" }, "FrameKey-0-4-3": { "COM4-StopBits": "1", "528 AO-MaxVolts": "5.0000", "XLIGHT Excitation Wheel-State": "1", "470 Shutter-Name": "AnalogIO", "Core-Shutter": "Vincent-D3", "555 AO-Demo": "No", "XLIGHT Dichroic-State": "2", "XYStage-StepSize-Y": "0.1000", "ZPositionUm": 198.455, "XYStage-StepSize-X": "0.1000", "PRIME-FirmwareVersion": "20.28", "PRIME-ClearCycles": "2", "520-Name": "DA Shutter", "405 Shutter-Name": "AnalogIO", "PIEZO-StepSize": "0.0050", "PRIME-ClearMode": "Pre-Exposure", "470 AO-MinVolts": "0.0000", "405 AO-SupportsTriggering": "1", "CameraChannelIndex": 0, "XYStage-StartSpeed": "500.0000", "COM9-StopBits": "2", "405 AO-Description": "NI DAC", "COM4-DelayBetweenCharsMs": "0.0000", "445 AO-Block voltage": "false", "520 AO-TriggerInputLine": "/Dev1/PFI0", "555 AO-Description": "NI DAC", "520 AO-TriggerSampleRate": "100000", "COM4-Verbose": "1", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM4-Name": "COM4", "405 Shutter-Volts": "0.0000", "Vincent-D3-Port": "COM1", "PRIME-PP 4 MIN ADU AFFECTED": "200", "470 Shutter-MinVolts": "0.0000", "PRIME-PP 3 ENABLED": "Yes", "470 AO-Description": "NI DAC", "PRIME-Description": "PVCAM API device adapter", "520 Shutter-MaxVolts": "5.0000", "520 Shutter-Volts": "0.0000", "528 AO-Sequenceable": "1", "PRIME-Binning": "1x1", "555 Shutter-MaxVolts": "5.0000", "528 Shutter-TriggerSequenceLength": "1000", "405 Shutter-Block voltage": "false", "XLIGHT Spin motor-State": "1", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "Vincent-D3-Time to open (ms)": "35.0000", "Vincent-D3-Time to close (ms)": "35.0000", "445 AO-TriggerSampleRate": "100000", "640 Shutter-IOChannel": "Dev1/ao14", "555 AO-Sequenceable": "1", "520 AO-Name": "AnalogIO", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "470 AO-Block voltage": "false", "COM1-Verbose": "1", "COM9-AnswerTimeout": "10000.0000", "528 Shutter-Name": "AnalogIO", "PRIME-Trigger Timeout (secs)": "10", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "NextFrame": 0, "470 AO-TriggerSequenceLength": "1000", "PRIME-TransposeXY": "0", "445 AO-TriggerSequenceLength": "1000", "COM1-Name": "COM1", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "COM9-DataBits": "8", "COM1-AnswerTimeout": "500.0000", "Camera": "", "TE2000-Version": "V2.50-V2.50-V2.10", "528 Shutter-Volts": "0.0000", "XLIGHT Hub-Name": "XLIGHT Hub", "PIEZO-Description": "Ludl stage driver adapter", "PositionName": "Pos34", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSampleRate": "100000", "PRIME-FanSpeedSetpoint": "High", "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "405 AO-TriggerSampleRate": "100000", "470 Shutter-Block voltage": "false", "470-State": "0", "COM3-Parity": "None", "405 AO-Volts": "0.0000", "PRIME-TransposeMirrorY": "0", "PRIME-ChipName": "GS144BSI", "470 AO-Volts": "0.0000", "PRIME-TransposeMirrorX": "0", "445 Shutter-SupportsTriggering": "0", "470 Shutter-SupportsTriggering": "0", "XLIGHT Hub-Description": "XLIGHT hub", "PRIME-PreampOffLimit": "10000", "470 AO-Sequenceable": "1", "528 Shutter-MaxVolts": "5.0000", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "Core-XYStage": "XYStage", "470 AO-TriggerSampleRate": "100000", "PRIME-Timing-PreTriggerDelayNs": "24672000.0000", "528-DA Device": "528 Shutter", "Focus-Description": "Nikon TE2000 focus adapter", "FilterBlock-State": "5", "520 AO-Demo": "No", "COM3-DelayBetweenCharsMs": "0.0000", "470 Shutter-Volts": "0.0000", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "PRIME-PP 2 THRESHOLD": "125", "PRIME-PP 4 THRESHOLD": "75", "XYStage-Acceleration": "4.0000", "470 Shutter-Demo": "No", "PRIME-Name": "PMPCIECam00", "640-DA Device": "640 Shutter", "405 AO-Block voltage": "false", "ElapsedTime-ms": 1419900, "PRIME-Offset": "100", "445 AO-IOChannel": "Dev1/ao1", "640 AO-MaxVolts": "5.0000", "PositionIndex": 34, "PRIME-PP 1 ENABLED": "Yes", "COM1-BaudRate": "9600", "640-State": "0", "COM3-Verbose": "1", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "LudlController-TransmissionDelay": "0", "PRIME-FullWellCapacity": "62000", "PRIME-SMARTStreamingEnabled": "No", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "640 Shutter-Description": "NI DAC", "528-Description": "DA device that is used as a shutter", "COM4-Description": "Serial port driver (boost:asio)", "405 Shutter-Description": "NI DAC", "TE2000-Name": "TE2000", "528 Shutter-Description": "NI DAC", "COM9-Description": "Serial port driver (boost:asio)", "LudlController-Name": "LudlController", "445 Shutter-TriggerSequenceLength": "1000", "PRIME-SerialNumber": "A18B203008", "445 AO-Volts": "0.0000", "PRIME-X-dimension": "1200", "PRIME-ShutterCloseDelay": "0", "XLIGHT Disk slider-State": "2", "Position": "Default", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 AO-IOChannel": "Dev1/ao5", "520 AO-IOChannel": "Dev1/ao4", "528 AO-TriggerSequenceLength": "1000", "405 Shutter-MaxVolts": "5.0000", "470-DA Device": "470 Shutter", "PRIME-FTCapable": "No", "Core-ChannelGroup": "Channels", "555 AO-SupportsTriggering": "0", "PRIME-Y-dimension": "1200", "Frame": 0, "555 Shutter-Sequenceable": "1", "PRIME-Timing-ExposureTimeNs": "499998000.0000", "Core-ImageProcessor": "", "528-State": "0", "520 AO-Block voltage": "false", "XLIGHT Excitation Wheel-Label": "Excitation-2", "640 Shutter-Demo": "No", "COM3-StopBits": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "520 Shutter-IOChannel": "Dev1/ao11", "Width": 1200, "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-SupportsTriggering": "0", "640 AO-TriggerSampleRate": "100000", "520-Description": "DA device that is used as a shutter", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "405 Shutter-Sequenceable": "1", "405 Shutter-SupportsTriggering": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-TriggerSampleRate": "100000", "640 Shutter-MaxVolts": "5.0000", "555 AO-MaxVolts": "5.0000", "520 AO-TriggerSequenceLength": "1000", "COM3-BaudRate": "9600", "COM9-BaudRate": "9600", "Vincent-D3-Channel #": "Ch. #1", "PRIME-PMode": "Normal", "OpticalPath-Label": "Left(100)", "BitDepth": 16, "555 AO-TriggerSequenceLength": "1000", "445 Shutter-Description": "NI DAC", "405 AO-Sequenceable": "1", "PRIME-AcquisitionMethod": "Callbacks", "555 AO-Name": "AnalogIO", "PRIME-ShutterMode": "Pre-Exposure", "COM3-Handshaking": "Off", "445 AO-SupportsTriggering": "0", "ROI": "0-0-1200-1200", "COM9-Verbose": "1", "PRIME-Color": "OFF", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "PRIME-ShutterOpenDelay": "0", "COM1-Parity": "None", "Slice": 3, "SlicePosition": -20, "COM9-DelayBetweenCharsMs": "0.0000", "555-Name": "DA Shutter", "PIEZO-LudlSingleAxisName": "B", "XLIGHT Emission Wheel-Label": "Empty", "640 Shutter-Name": "AnalogIO", "640 AO-Sequenceable": "1", "520 Shutter-Demo": "No", "528 Shutter-Demo": "No", "470-Name": "DA Shutter", "520-DA Device": "520 Shutter", "XYStage-Description": "Ludl XY stage driver adapter", "445 Shutter-MinVolts": "0.0000", "528 AO-TriggerSampleRate": "100000", "405 AO-MinVolts": "0.0000", "405 Shutter-TriggerSequenceLength": "1000", "Height": 1200, "555 AO-Block voltage": "false", "640 Shutter-Volts": "0.0000", "PixelType": "GRAY16", "445 Shutter-Block voltage": "false", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "520 Shutter-TriggerSequenceLength": "1000", "COM9-Name": "COM9", "Core-SLM": "", "555 AO-Volts": "0.0000", "555 Shutter-Block voltage": "false", "XYStage-Speed": "1800.0000", "520 AO-MaxVolts": "5.0000", "528 Shutter-SupportsTriggering": "0", "COM1-Description": "Serial port driver (boost:asio)", "PRIME-MetadataEnabled": "No", "520 Shutter-MinVolts": "0.0000", "470 Shutter-Sequenceable": "1", "PRIME-PreampDelay": "0", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-MinVolts": "0.0000", "ChannelIndex": 4, "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Core-AutoShutter": "1", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "Exposure-ms": 20, "PRIME-PP 2 ENABLED": "Yes", "640 Shutter-TriggerSampleRate": "100000", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "470 Shutter-IOChannel": "Dev1/ao10", "405 AO-Demo": "No", "PixelSizeUm": 1.1, "470 AO-Demo": "No", "470 Shutter-MaxVolts": "5.0000", "Vincent-D3-Name": "Vincent-D3", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FileName": "img_000000000_P-DIC_003.tif", "LudlController-Reset": "Operate", "PRIME-Exposure": "20.00", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "445-Name": "DA Shutter", "555 Shutter-Name": "AnalogIO", "520 Shutter-TriggerSampleRate": "100000", "445 Shutter-IOChannel": "Dev1/ao9", "PRIME-TransposeCorrection": "0", "XYStage-StepSize": "0.1000", "445-Description": "DA device that is used as a shutter", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 AO-IOChannel": "Dev1/ao7", "520 Shutter-Name": "AnalogIO", "COM3-DataBits": "8", "640 AO-Volts": "0.0000", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "555 Shutter-IOChannel": "Dev1/ao13", "WaitInterval": null, "520 Shutter-Sequenceable": "1", "YPositionUm": 545.6, "445-State": "0", "445-DA Device": "445 Shutter", "PRIME-CircularBufferAutoSize": "ON", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "555 AO-TriggerSampleRate": "100000", "445 Shutter-Volts": "0.0000", "XLIGHT Hub-Port": "COM3", "Core-AutoFocus": "", "COM4-Handshaking": "Off", "528 Shutter-MinVolts": "0.0000", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "445 Shutter-Name": "AnalogIO", "528 Shutter-Sequenceable": "1", "XYStage-TransposeMirrorX": "0", "XYStage-TransposeMirrorY": "0", "PRIME-ReadoutRate": "100MHz 16bit", "XYStage-Name": "Stage", "640 AO-SupportsTriggering": "0", "640-Name": "DA Shutter", "640 Shutter-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "640 AO-Block voltage": "false", "445 Shutter-Sequenceable": "1", "470 AO-MaxVolts": "5.0000", "COM4-BaudRate": "9600", "470-Description": "DA device that is used as a shutter", "520 Shutter-Description": "NI DAC", "PRIME-CCDTemperature": "-14.8500", "Time": "2019-12-19 09:59:50 -0500", "640 AO-TriggerSequenceLength": "1000", "470 AO-Name": "AnalogIO", "XLIGHT Disk slider-Label": "Disk Pos 40um", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "Binning": "1x1", "TE2000-ModelType": "1", "COM3-AnswerTimeout": "500.0000", "555 Shutter-SupportsTriggering": "0", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "TE2000-Port": "COM4", "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Description": "NI DAC", "COM4-AnswerTimeout": "500.0000", "COM1-DelayBetweenCharsMs": "0.0000", "FilterBlock-Label": "Confocal", "445 AO-Name": "AnalogIO", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-Timing-ReadoutTimeNs": "24672000.0000", "528 AO-Demo": "No", "520 AO-Sequenceable": "1", "555-Description": "DA device that is used as a shutter", "405-DA Device": "405 Shutter", "405 AO-TriggerSequenceLength": "1000", "640 AO-MinVolts": "0.0000", "640 Shutter-Block voltage": "false", "FrameIndex": 0, "PRIME-Port": "CMOS", "555 AO-MinVolts": "0.0000", "COM3-Description": "Serial port driver (boost:asio)", "528 AO-Description": "NI DAC", "555 AO-IOChannel": "Dev1/ao6", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "555 AO-TriggerInputLine": "/Dev1/PFI0", "PRIME-ActualInterval-ms": "390.5200", "LudlController-Port": "COM9", "445 Shutter-Demo": "No", "PRIME-Trigger-Expose Out-Mux": "1", "SliceIndex": 3, "PRIME-CCDTemperatureSetPoint": "-15.0000", "405 Shutter-MinVolts": "0.0000", "PRIME-ExposeOutMode": "First Row", "AxisPositions": null, "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Volts": "0.0000", "528-Name": "DA Shutter", "555-State": "0", "XLIGHT Dichroic-Label": "Di03-R635-t1", "445 AO-Sequenceable": "1", "405-Name": "DA Shutter", "405 AO-IOChannel": "Dev1/ao0", "Core-Camera": "PRIME", "Focus-Name": "Focus", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "405 Shutter-IOChannel": "Dev1/ao8", "640 AO-Demo": "No", "640 Shutter-SupportsTriggering": "0", "445 AO-Demo": "No", "555-DA Device": "555 Shutter", "470 Shutter-Description": "NI DAC", "528 AO-Volts": "0.0000", "COM1-DataBits": "8", "Vincent-D3-Address": "x", "520 AO-SupportsTriggering": "0", "405-Description": "DA device that is used as a shutter", "640 Shutter-MinVolts": "0.0000", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "405 AO-Name": "AnalogIO", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "640 AO-TriggerInputLine": "/Dev1/PFI0", "405 AO-TriggerInputLine": "/Dev1/PFI0", "640-Description": "DA device that is used as a shutter", "445 Shutter-TriggerSampleRate": "100000", "470 Shutter-TriggerSequenceLength": "1000", "COM1-StopBits": "1", "520 Shutter-SupportsTriggering": "0", "405-State": "0", "Core-Initialize": "1", "XLIGHT Emission Wheel-State": "3", "520 AO-Description": "NI DAC", "528 AO-TriggerInputLine": "/Dev1/PFI0", "445 Shutter-MaxVolts": "5.0000", "COM9-Handshaking": "Off", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "XLIGHT Spin motor-Label": "On (spinning)", "PRIME-PP 5 ENABLED": "No", "UUID": "344da960-0071-44b6-b3d3-846fdb249969", "PRIME-TriggerMode": "Internal Trigger", "COM1-Handshaking": "Off", "PRIME-Timing-PostTriggerDelayNs": "49344000.0000", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "Focus-Position": "17741.5000", "528 Shutter-Block voltage": "false", "Channel": "P-DIC", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "555 Shutter-Demo": "No", "405 Shutter-Demo": "No", "528 AO-Name": "AnalogIO", "520-State": "0", "Core-Galvo": "", "405 Shutter-TriggerSampleRate": "100000", "Focus-FocusStepSize_(nm)": "100", "PRIME-PixelType": "16bit", "COM4-DataBits": "8", "470 AO-IOChannel": "Dev1/ao2", "520 AO-MinVolts": "0.0000", "COM9-Parity": "None", "PRIME-CircularBufferEnabled": "ON", "PRIME-PP 6 Reset": "No", "640 AO-Description": "NI DAC", "Source": "PRIME", "445 AO-MinVolts": "0.0000", "PIEZO-Name": "Stage", "445 AO-Description": "NI DAC", "PRIME-CircularBufferFrameCount": "46", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "520 AO-Volts": "0.0000", "528 Shutter-TriggerSampleRate": "100000", "XPositionUm": -1324.9, "OpticalPath-State": "4", "PRIME-PP 3 THRESHOLD": "80", "PRIME-PVCAM Version": "3.7.1", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Gain": "1-HDR", "640 Shutter-TriggerSequenceLength": "1000", "528 AO-SupportsTriggering": "0", "445 AO-TriggerInputLine": "/Dev1/PFI0", "OpticalPath-Name": "OpticalPath", "PRIME-PP 4 ENABLED": "Yes", "PRIME-PVCAM Adapter Version": "1.3.29", "COM3-Name": "COM3", "405 AO-MaxVolts": "5.0000", "520 Shutter-Block voltage": "false", "PRIME-Timing-ClearingTimeNs": "49344000.0000", "Core-Focus": "PIEZO", "528 AO-Block voltage": "false", "TE2000-Description": "Nikon TE2000 hub", "528 AO-MinVolts": "0.0000", "COM4-Parity": "None", "Summary": { "Slices": 11, "ChContrastMin": [ 0, 0, 0, 0, 0, 0 ], "PixelAspect": 1, "SlicesFirst": true, "Interval_ms": 0, "Time": "2019-12-19 09:59:21 -0500", "Prefix": "seq_13", "InitialPositionList": [ { "Label": "Pos0", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.21], "Focus": [17741.5], "XYStage": [ 44542.200000000004, 31636.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos1", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.22], "Focus": [17741.4], "XYStage": [ 43801.100000000006, 31637.9 ] }, "GridColumnIndex": 0 }, { "Label": "Pos2", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.5], "XYStage": [ 38561.3, 32040.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos3", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.185], "Focus": [17741.3], "XYStage": [ 37698.4, 32041.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos4", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.16], "Focus": [17741.5], "XYStage": [ 32375.4, 31758.9 ] }, "GridColumnIndex": 0 }, { "Label": "Pos5", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.17000000000002], "Focus": [17741.3], "XYStage": [ 31770.7, 31758.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos6", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.4], "XYStage": [ 26536.800000000003, 31068.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos7", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.205], "Focus": [17741.4], "XYStage": [ 25420.2, 31068.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos8", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.85], "Focus": [17741.3], "XYStage": [ 21059, 31508 ] }, "GridColumnIndex": 0 }, { "Label": "Pos9", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.82], "Focus": [17741.3], "XYStage": [ 19731.9, 31506.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos10", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.79], "Focus": [17741.4], "XYStage": [ 14528.5, 32007.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos11", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.83], "Focus": [17741.2], "XYStage": [ 13830.300000000001, 32009.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos12", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.79], "Focus": [17741.3], "XYStage": [ 8775.300000000001, 32017.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos13", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.815], "Focus": [17741.3], "XYStage": [ 8109.200000000001, 32008.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos14", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.805], "Focus": [17741.3], "XYStage": [ 3483.3, 31908.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos15", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.82], "Focus": [17741.2], "XYStage": [ 2686.8, 31903.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos16", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [211.48000000000002], "Focus": [17741.1], "XYStage": [ -1978.9, 31973.600000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos17", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [211.51500000000001], "Focus": [17741.1], "XYStage": [ -2957.8, 31982.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos18", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.195], "Focus": [17741.5], "XYStage": [ 47747.4, -576.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos19", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.23000000000002], "Focus": [17741.6], "XYStage": [ 47053.9, -576.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos20", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.205], "Focus": [17741.4], "XYStage": [ 41871.200000000004, -479.8 ] }, "GridColumnIndex": 0 }, { "Label": "Pos21", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.4], "XYStage": [ 40882.600000000006, -392 ] }, "GridColumnIndex": 0 }, { "Label": "Pos22", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.24], "Focus": [17741.4], "XYStage": [ 35905.6, -135.3 ] }, "GridColumnIndex": 0 }, { "Label": "Pos23", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.195], "Focus": [17741.5], "XYStage": [ 34990.9, -132.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos24", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.46], "Focus": [17741.4], "XYStage": [ 29678.100000000002, -958.1 ] }, "GridColumnIndex": 0 }, { "Label": "Pos25", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.44], "Focus": [17741.5], "XYStage": [ 28983.600000000002, -939.4000000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos26", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.475], "Focus": [17741.5], "XYStage": [ 23019.7, -122 ] }, "GridColumnIndex": 0 }, { "Label": "Pos27", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.44], "Focus": [17741.7], "XYStage": [ 22428, -116.80000000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos28", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.46], "Focus": [17741.7], "XYStage": [ 16364.900000000001, -181.10000000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos29", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.48000000000002], "Focus": [17741.5], "XYStage": [ 15712.1, -180 ] }, "GridColumnIndex": 0 }, { "Label": "Pos30", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.45000000000002], "Focus": [17741.7], "XYStage": [ 10346.1, 77.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos31", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.49], "Focus": [17741.4], "XYStage": [ 9755.5, 85.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos32", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.475], "Focus": [17741.5], "XYStage": [ 4775.5, 293.90000000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos33", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.445], "Focus": [17741.4], "XYStage": [ 3954.9, 293.90000000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos34", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.455], "Focus": [17741.4], "XYStage": [ -1324.9, 545.6 ] }, "GridColumnIndex": 0 }, { "Label": "Pos35", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.445], "Focus": [17741.3], "XYStage": [ -2097, 576.6 ] }, "GridColumnIndex": 0 } ], "z-step_um": 10, "GridRow": 0, "BitDepth": 16, "Depth": 2, "Channels": 6, "Source": "Micro-Manager", "ChColors": [ -16711681, -256, -65281, -1, -1, -16776961 ], "MetadataVersion": 10, "KeepShutterOpenChannels": true, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536, 65536 ], "CustomIntervals_ms": [], "Height": 1200, "MicroManagerVersion": "1.4.23 20180219", "GridColumn": 0, "UUID": "18808e7c-f2e6-47b3-90d8-1d779ce87dbd", "PixelType": "GRAY16", "Width": 1200, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\Yan\\20191218-YC113-S7S8-miseq", "Frames": 1, "Comment": "", "UserName": "Nikon", "PixelSize_um": 1.1, "ROI": [ 0, 0, 1200, 1200 ], "Date": "2019-12-19", "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC", "P-DAPI-high" ], "KeepShutterOpenSlices": true, "IJType": 1, "ComputerName": "ZadorConfocal2", "Positions": 36, "PositionIndex": 34, "CameraTimeout": 20000, "TimeFirst": false }, "640 AO-Name": "AnalogIO", "Vincent-D3-Command": "Close" }, "FrameKey-0-4-4": { "COM4-StopBits": "1", "528 AO-MaxVolts": "5.0000", "XLIGHT Excitation Wheel-State": "1", "470 Shutter-Name": "AnalogIO", "Core-Shutter": "Vincent-D3", "555 AO-Demo": "No", "XLIGHT Dichroic-State": "2", "XYStage-StepSize-Y": "0.1000", "ZPositionUm": 208.455, "XYStage-StepSize-X": "0.1000", "PRIME-FirmwareVersion": "20.28", "PRIME-ClearCycles": "2", "520-Name": "DA Shutter", "405 Shutter-Name": "AnalogIO", "PIEZO-StepSize": "0.0050", "PRIME-ClearMode": "Pre-Exposure", "470 AO-MinVolts": "0.0000", "405 AO-SupportsTriggering": "1", "CameraChannelIndex": 0, "XYStage-StartSpeed": "500.0000", "COM9-StopBits": "2", "405 AO-Description": "NI DAC", "COM4-DelayBetweenCharsMs": "0.0000", "445 AO-Block voltage": "false", "520 AO-TriggerInputLine": "/Dev1/PFI0", "555 AO-Description": "NI DAC", "520 AO-TriggerSampleRate": "100000", "COM4-Verbose": "1", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM4-Name": "COM4", "405 Shutter-Volts": "0.0000", "Vincent-D3-Port": "COM1", "PRIME-PP 4 MIN ADU AFFECTED": "200", "470 Shutter-MinVolts": "0.0000", "PRIME-PP 3 ENABLED": "Yes", "470 AO-Description": "NI DAC", "PRIME-Description": "PVCAM API device adapter", "520 Shutter-MaxVolts": "5.0000", "520 Shutter-Volts": "0.0000", "528 AO-Sequenceable": "1", "PRIME-Binning": "1x1", "555 Shutter-MaxVolts": "5.0000", "528 Shutter-TriggerSequenceLength": "1000", "405 Shutter-Block voltage": "false", "XLIGHT Spin motor-State": "1", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "Vincent-D3-Time to open (ms)": "35.0000", "Vincent-D3-Time to close (ms)": "35.0000", "445 AO-TriggerSampleRate": "100000", "640 Shutter-IOChannel": "Dev1/ao14", "555 AO-Sequenceable": "1", "520 AO-Name": "AnalogIO", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "470 AO-Block voltage": "false", "COM1-Verbose": "1", "COM9-AnswerTimeout": "10000.0000", "528 Shutter-Name": "AnalogIO", "PRIME-Trigger Timeout (secs)": "10", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "NextFrame": 0, "470 AO-TriggerSequenceLength": "1000", "PRIME-TransposeXY": "0", "445 AO-TriggerSequenceLength": "1000", "COM1-Name": "COM1", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "COM9-DataBits": "8", "COM1-AnswerTimeout": "500.0000", "Camera": "", "TE2000-Version": "V2.50-V2.50-V2.10", "528 Shutter-Volts": "0.0000", "XLIGHT Hub-Name": "XLIGHT Hub", "PIEZO-Description": "Ludl stage driver adapter", "PositionName": "Pos34", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSampleRate": "100000", "PRIME-FanSpeedSetpoint": "High", "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "405 AO-TriggerSampleRate": "100000", "470 Shutter-Block voltage": "false", "470-State": "0", "COM3-Parity": "None", "405 AO-Volts": "0.0000", "PRIME-TransposeMirrorY": "0", "PRIME-ChipName": "GS144BSI", "470 AO-Volts": "0.0000", "PRIME-TransposeMirrorX": "0", "445 Shutter-SupportsTriggering": "0", "470 Shutter-SupportsTriggering": "0", "XLIGHT Hub-Description": "XLIGHT hub", "PRIME-PreampOffLimit": "10000", "470 AO-Sequenceable": "1", "528 Shutter-MaxVolts": "5.0000", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "Core-XYStage": "XYStage", "470 AO-TriggerSampleRate": "100000", "PRIME-Timing-PreTriggerDelayNs": "24672000.0000", "528-DA Device": "528 Shutter", "Focus-Description": "Nikon TE2000 focus adapter", "FilterBlock-State": "5", "520 AO-Demo": "No", "COM3-DelayBetweenCharsMs": "0.0000", "470 Shutter-Volts": "0.0000", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "PRIME-PP 2 THRESHOLD": "125", "PRIME-PP 4 THRESHOLD": "75", "XYStage-Acceleration": "4.0000", "470 Shutter-Demo": "No", "PRIME-Name": "PMPCIECam00", "640-DA Device": "640 Shutter", "405 AO-Block voltage": "false", "ElapsedTime-ms": 1420152, "PRIME-Offset": "100", "445 AO-IOChannel": "Dev1/ao1", "640 AO-MaxVolts": "5.0000", "PositionIndex": 34, "PRIME-PP 1 ENABLED": "Yes", "COM1-BaudRate": "9600", "640-State": "0", "COM3-Verbose": "1", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "LudlController-TransmissionDelay": "0", "PRIME-FullWellCapacity": "62000", "PRIME-SMARTStreamingEnabled": "No", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "640 Shutter-Description": "NI DAC", "528-Description": "DA device that is used as a shutter", "COM4-Description": "Serial port driver (boost:asio)", "405 Shutter-Description": "NI DAC", "TE2000-Name": "TE2000", "528 Shutter-Description": "NI DAC", "COM9-Description": "Serial port driver (boost:asio)", "LudlController-Name": "LudlController", "445 Shutter-TriggerSequenceLength": "1000", "PRIME-SerialNumber": "A18B203008", "445 AO-Volts": "0.0000", "PRIME-X-dimension": "1200", "PRIME-ShutterCloseDelay": "0", "XLIGHT Disk slider-State": "2", "Position": "Default", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 AO-IOChannel": "Dev1/ao5", "520 AO-IOChannel": "Dev1/ao4", "528 AO-TriggerSequenceLength": "1000", "405 Shutter-MaxVolts": "5.0000", "470-DA Device": "470 Shutter", "PRIME-FTCapable": "No", "Core-ChannelGroup": "Channels", "555 AO-SupportsTriggering": "0", "PRIME-Y-dimension": "1200", "Frame": 0, "555 Shutter-Sequenceable": "1", "PRIME-Timing-ExposureTimeNs": "499998000.0000", "Core-ImageProcessor": "", "528-State": "0", "520 AO-Block voltage": "false", "XLIGHT Excitation Wheel-Label": "Excitation-2", "640 Shutter-Demo": "No", "COM3-StopBits": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "520 Shutter-IOChannel": "Dev1/ao11", "Width": 1200, "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-SupportsTriggering": "0", "640 AO-TriggerSampleRate": "100000", "520-Description": "DA device that is used as a shutter", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "405 Shutter-Sequenceable": "1", "405 Shutter-SupportsTriggering": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-TriggerSampleRate": "100000", "640 Shutter-MaxVolts": "5.0000", "555 AO-MaxVolts": "5.0000", "520 AO-TriggerSequenceLength": "1000", "COM3-BaudRate": "9600", "COM9-BaudRate": "9600", "Vincent-D3-Channel #": "Ch. #1", "PRIME-PMode": "Normal", "OpticalPath-Label": "Left(100)", "BitDepth": 16, "555 AO-TriggerSequenceLength": "1000", "445 Shutter-Description": "NI DAC", "405 AO-Sequenceable": "1", "PRIME-AcquisitionMethod": "Callbacks", "555 AO-Name": "AnalogIO", "PRIME-ShutterMode": "Pre-Exposure", "COM3-Handshaking": "Off", "445 AO-SupportsTriggering": "0", "ROI": "0-0-1200-1200", "COM9-Verbose": "1", "PRIME-Color": "OFF", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "PRIME-ShutterOpenDelay": "0", "COM1-Parity": "None", "Slice": 4, "SlicePosition": -10, "COM9-DelayBetweenCharsMs": "0.0000", "555-Name": "DA Shutter", "PIEZO-LudlSingleAxisName": "B", "XLIGHT Emission Wheel-Label": "Empty", "640 Shutter-Name": "AnalogIO", "640 AO-Sequenceable": "1", "520 Shutter-Demo": "No", "528 Shutter-Demo": "No", "470-Name": "DA Shutter", "520-DA Device": "520 Shutter", "XYStage-Description": "Ludl XY stage driver adapter", "445 Shutter-MinVolts": "0.0000", "528 AO-TriggerSampleRate": "100000", "405 AO-MinVolts": "0.0000", "405 Shutter-TriggerSequenceLength": "1000", "Height": 1200, "555 AO-Block voltage": "false", "640 Shutter-Volts": "0.0000", "PixelType": "GRAY16", "445 Shutter-Block voltage": "false", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "520 Shutter-TriggerSequenceLength": "1000", "COM9-Name": "COM9", "Core-SLM": "", "555 AO-Volts": "0.0000", "555 Shutter-Block voltage": "false", "XYStage-Speed": "1800.0000", "520 AO-MaxVolts": "5.0000", "528 Shutter-SupportsTriggering": "0", "COM1-Description": "Serial port driver (boost:asio)", "PRIME-MetadataEnabled": "No", "520 Shutter-MinVolts": "0.0000", "470 Shutter-Sequenceable": "1", "PRIME-PreampDelay": "0", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-MinVolts": "0.0000", "ChannelIndex": 4, "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Core-AutoShutter": "1", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "Exposure-ms": 20, "PRIME-PP 2 ENABLED": "Yes", "640 Shutter-TriggerSampleRate": "100000", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "470 Shutter-IOChannel": "Dev1/ao10", "405 AO-Demo": "No", "PixelSizeUm": 1.1, "470 AO-Demo": "No", "470 Shutter-MaxVolts": "5.0000", "Vincent-D3-Name": "Vincent-D3", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FileName": "img_000000000_P-DIC_004.tif", "LudlController-Reset": "Operate", "PRIME-Exposure": "20.00", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "445-Name": "DA Shutter", "555 Shutter-Name": "AnalogIO", "520 Shutter-TriggerSampleRate": "100000", "445 Shutter-IOChannel": "Dev1/ao9", "PRIME-TransposeCorrection": "0", "XYStage-StepSize": "0.1000", "445-Description": "DA device that is used as a shutter", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 AO-IOChannel": "Dev1/ao7", "520 Shutter-Name": "AnalogIO", "COM3-DataBits": "8", "640 AO-Volts": "0.0000", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "555 Shutter-IOChannel": "Dev1/ao13", "WaitInterval": null, "520 Shutter-Sequenceable": "1", "YPositionUm": 545.6, "445-State": "0", "445-DA Device": "445 Shutter", "PRIME-CircularBufferAutoSize": "ON", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "555 AO-TriggerSampleRate": "100000", "445 Shutter-Volts": "0.0000", "XLIGHT Hub-Port": "COM3", "Core-AutoFocus": "", "COM4-Handshaking": "Off", "528 Shutter-MinVolts": "0.0000", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "445 Shutter-Name": "AnalogIO", "528 Shutter-Sequenceable": "1", "XYStage-TransposeMirrorX": "0", "XYStage-TransposeMirrorY": "0", "PRIME-ReadoutRate": "100MHz 16bit", "XYStage-Name": "Stage", "640 AO-SupportsTriggering": "0", "640-Name": "DA Shutter", "640 Shutter-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "640 AO-Block voltage": "false", "445 Shutter-Sequenceable": "1", "470 AO-MaxVolts": "5.0000", "COM4-BaudRate": "9600", "470-Description": "DA device that is used as a shutter", "520 Shutter-Description": "NI DAC", "PRIME-CCDTemperature": "-14.8500", "Time": "2019-12-19 09:59:50 -0500", "640 AO-TriggerSequenceLength": "1000", "470 AO-Name": "AnalogIO", "XLIGHT Disk slider-Label": "Disk Pos 40um", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "Binning": "1x1", "TE2000-ModelType": "1", "COM3-AnswerTimeout": "500.0000", "555 Shutter-SupportsTriggering": "0", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "TE2000-Port": "COM4", "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Description": "NI DAC", "COM4-AnswerTimeout": "500.0000", "COM1-DelayBetweenCharsMs": "0.0000", "FilterBlock-Label": "Confocal", "445 AO-Name": "AnalogIO", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-Timing-ReadoutTimeNs": "24672000.0000", "528 AO-Demo": "No", "520 AO-Sequenceable": "1", "555-Description": "DA device that is used as a shutter", "405-DA Device": "405 Shutter", "405 AO-TriggerSequenceLength": "1000", "640 AO-MinVolts": "0.0000", "640 Shutter-Block voltage": "false", "FrameIndex": 0, "PRIME-Port": "CMOS", "555 AO-MinVolts": "0.0000", "COM3-Description": "Serial port driver (boost:asio)", "528 AO-Description": "NI DAC", "555 AO-IOChannel": "Dev1/ao6", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "555 AO-TriggerInputLine": "/Dev1/PFI0", "PRIME-ActualInterval-ms": "390.5200", "LudlController-Port": "COM9", "445 Shutter-Demo": "No", "PRIME-Trigger-Expose Out-Mux": "1", "SliceIndex": 4, "PRIME-CCDTemperatureSetPoint": "-15.0000", "405 Shutter-MinVolts": "0.0000", "PRIME-ExposeOutMode": "First Row", "AxisPositions": null, "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Volts": "0.0000", "528-Name": "DA Shutter", "555-State": "0", "XLIGHT Dichroic-Label": "Di03-R635-t1", "445 AO-Sequenceable": "1", "405-Name": "DA Shutter", "405 AO-IOChannel": "Dev1/ao0", "Core-Camera": "PRIME", "Focus-Name": "Focus", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "405 Shutter-IOChannel": "Dev1/ao8", "640 AO-Demo": "No", "640 Shutter-SupportsTriggering": "0", "445 AO-Demo": "No", "555-DA Device": "555 Shutter", "470 Shutter-Description": "NI DAC", "528 AO-Volts": "0.0000", "COM1-DataBits": "8", "Vincent-D3-Address": "x", "520 AO-SupportsTriggering": "0", "405-Description": "DA device that is used as a shutter", "640 Shutter-MinVolts": "0.0000", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "405 AO-Name": "AnalogIO", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "640 AO-TriggerInputLine": "/Dev1/PFI0", "405 AO-TriggerInputLine": "/Dev1/PFI0", "640-Description": "DA device that is used as a shutter", "445 Shutter-TriggerSampleRate": "100000", "470 Shutter-TriggerSequenceLength": "1000", "COM1-StopBits": "1", "520 Shutter-SupportsTriggering": "0", "405-State": "0", "Core-Initialize": "1", "XLIGHT Emission Wheel-State": "3", "520 AO-Description": "NI DAC", "528 AO-TriggerInputLine": "/Dev1/PFI0", "445 Shutter-MaxVolts": "5.0000", "COM9-Handshaking": "Off", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "XLIGHT Spin motor-Label": "On (spinning)", "PRIME-PP 5 ENABLED": "No", "UUID": "c001a935-2477-4a6c-ba83-7e6d58477a65", "PRIME-TriggerMode": "Internal Trigger", "COM1-Handshaking": "Off", "PRIME-Timing-PostTriggerDelayNs": "49344000.0000", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "Focus-Position": "17741.5000", "528 Shutter-Block voltage": "false", "Channel": "P-DIC", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "555 Shutter-Demo": "No", "405 Shutter-Demo": "No", "528 AO-Name": "AnalogIO", "520-State": "0", "Core-Galvo": "", "405 Shutter-TriggerSampleRate": "100000", "Focus-FocusStepSize_(nm)": "100", "PRIME-PixelType": "16bit", "COM4-DataBits": "8", "470 AO-IOChannel": "Dev1/ao2", "520 AO-MinVolts": "0.0000", "COM9-Parity": "None", "PRIME-CircularBufferEnabled": "ON", "PRIME-PP 6 Reset": "No", "640 AO-Description": "NI DAC", "Source": "PRIME", "445 AO-MinVolts": "0.0000", "PIEZO-Name": "Stage", "445 AO-Description": "NI DAC", "PRIME-CircularBufferFrameCount": "46", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "520 AO-Volts": "0.0000", "528 Shutter-TriggerSampleRate": "100000", "XPositionUm": -1324.9, "OpticalPath-State": "4", "PRIME-PP 3 THRESHOLD": "80", "PRIME-PVCAM Version": "3.7.1", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Gain": "1-HDR", "640 Shutter-TriggerSequenceLength": "1000", "528 AO-SupportsTriggering": "0", "445 AO-TriggerInputLine": "/Dev1/PFI0", "OpticalPath-Name": "OpticalPath", "PRIME-PP 4 ENABLED": "Yes", "PRIME-PVCAM Adapter Version": "1.3.29", "COM3-Name": "COM3", "405 AO-MaxVolts": "5.0000", "520 Shutter-Block voltage": "false", "PRIME-Timing-ClearingTimeNs": "49344000.0000", "Core-Focus": "PIEZO", "528 AO-Block voltage": "false", "TE2000-Description": "Nikon TE2000 hub", "528 AO-MinVolts": "0.0000", "COM4-Parity": "None", "Summary": { "Slices": 11, "ChContrastMin": [ 0, 0, 0, 0, 0, 0 ], "PixelAspect": 1, "SlicesFirst": true, "Interval_ms": 0, "Time": "2019-12-19 09:59:21 -0500", "Prefix": "seq_13", "InitialPositionList": [ { "Label": "Pos0", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.21], "Focus": [17741.5], "XYStage": [ 44542.200000000004, 31636.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos1", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.22], "Focus": [17741.4], "XYStage": [ 43801.100000000006, 31637.9 ] }, "GridColumnIndex": 0 }, { "Label": "Pos2", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.5], "XYStage": [ 38561.3, 32040.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos3", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.185], "Focus": [17741.3], "XYStage": [ 37698.4, 32041.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos4", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.16], "Focus": [17741.5], "XYStage": [ 32375.4, 31758.9 ] }, "GridColumnIndex": 0 }, { "Label": "Pos5", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.17000000000002], "Focus": [17741.3], "XYStage": [ 31770.7, 31758.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos6", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.4], "XYStage": [ 26536.800000000003, 31068.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos7", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.205], "Focus": [17741.4], "XYStage": [ 25420.2, 31068.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos8", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.85], "Focus": [17741.3], "XYStage": [ 21059, 31508 ] }, "GridColumnIndex": 0 }, { "Label": "Pos9", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.82], "Focus": [17741.3], "XYStage": [ 19731.9, 31506.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos10", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.79], "Focus": [17741.4], "XYStage": [ 14528.5, 32007.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos11", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.83], "Focus": [17741.2], "XYStage": [ 13830.300000000001, 32009.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos12", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.79], "Focus": [17741.3], "XYStage": [ 8775.300000000001, 32017.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos13", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.815], "Focus": [17741.3], "XYStage": [ 8109.200000000001, 32008.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos14", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.805], "Focus": [17741.3], "XYStage": [ 3483.3, 31908.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos15", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.82], "Focus": [17741.2], "XYStage": [ 2686.8, 31903.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos16", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [211.48000000000002], "Focus": [17741.1], "XYStage": [ -1978.9, 31973.600000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos17", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [211.51500000000001], "Focus": [17741.1], "XYStage": [ -2957.8, 31982.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos18", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.195], "Focus": [17741.5], "XYStage": [ 47747.4, -576.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos19", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.23000000000002], "Focus": [17741.6], "XYStage": [ 47053.9, -576.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos20", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.205], "Focus": [17741.4], "XYStage": [ 41871.200000000004, -479.8 ] }, "GridColumnIndex": 0 }, { "Label": "Pos21", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.4], "XYStage": [ 40882.600000000006, -392 ] }, "GridColumnIndex": 0 }, { "Label": "Pos22", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.24], "Focus": [17741.4], "XYStage": [ 35905.6, -135.3 ] }, "GridColumnIndex": 0 }, { "Label": "Pos23", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.195], "Focus": [17741.5], "XYStage": [ 34990.9, -132.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos24", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.46], "Focus": [17741.4], "XYStage": [ 29678.100000000002, -958.1 ] }, "GridColumnIndex": 0 }, { "Label": "Pos25", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.44], "Focus": [17741.5], "XYStage": [ 28983.600000000002, -939.4000000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos26", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.475], "Focus": [17741.5], "XYStage": [ 23019.7, -122 ] }, "GridColumnIndex": 0 }, { "Label": "Pos27", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.44], "Focus": [17741.7], "XYStage": [ 22428, -116.80000000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos28", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.46], "Focus": [17741.7], "XYStage": [ 16364.900000000001, -181.10000000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos29", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.48000000000002], "Focus": [17741.5], "XYStage": [ 15712.1, -180 ] }, "GridColumnIndex": 0 }, { "Label": "Pos30", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.45000000000002], "Focus": [17741.7], "XYStage": [ 10346.1, 77.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos31", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.49], "Focus": [17741.4], "XYStage": [ 9755.5, 85.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos32", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.475], "Focus": [17741.5], "XYStage": [ 4775.5, 293.90000000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos33", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.445], "Focus": [17741.4], "XYStage": [ 3954.9, 293.90000000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos34", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.455], "Focus": [17741.4], "XYStage": [ -1324.9, 545.6 ] }, "GridColumnIndex": 0 }, { "Label": "Pos35", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.445], "Focus": [17741.3], "XYStage": [ -2097, 576.6 ] }, "GridColumnIndex": 0 } ], "z-step_um": 10, "GridRow": 0, "BitDepth": 16, "Depth": 2, "Channels": 6, "Source": "Micro-Manager", "ChColors": [ -16711681, -256, -65281, -1, -1, -16776961 ], "MetadataVersion": 10, "KeepShutterOpenChannels": true, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536, 65536 ], "CustomIntervals_ms": [], "Height": 1200, "MicroManagerVersion": "1.4.23 20180219", "GridColumn": 0, "UUID": "18808e7c-f2e6-47b3-90d8-1d779ce87dbd", "PixelType": "GRAY16", "Width": 1200, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\Yan\\20191218-YC113-S7S8-miseq", "Frames": 1, "Comment": "", "UserName": "Nikon", "PixelSize_um": 1.1, "ROI": [ 0, 0, 1200, 1200 ], "Date": "2019-12-19", "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC", "P-DAPI-high" ], "KeepShutterOpenSlices": true, "IJType": 1, "ComputerName": "ZadorConfocal2", "Positions": 36, "PositionIndex": 34, "CameraTimeout": 20000, "TimeFirst": false }, "640 AO-Name": "AnalogIO", "Vincent-D3-Command": "Close" }, "FrameKey-0-4-5": { "COM4-StopBits": "1", "528 AO-MaxVolts": "5.0000", "XLIGHT Excitation Wheel-State": "1", "470 Shutter-Name": "AnalogIO", "Core-Shutter": "Vincent-D3", "555 AO-Demo": "No", "XLIGHT Dichroic-State": "2", "XYStage-StepSize-Y": "0.1000", "ZPositionUm": 218.455, "XYStage-StepSize-X": "0.1000", "PRIME-FirmwareVersion": "20.28", "PRIME-ClearCycles": "2", "520-Name": "DA Shutter", "405 Shutter-Name": "AnalogIO", "PIEZO-StepSize": "0.0050", "PRIME-ClearMode": "Pre-Exposure", "470 AO-MinVolts": "0.0000", "405 AO-SupportsTriggering": "1", "CameraChannelIndex": 0, "XYStage-StartSpeed": "500.0000", "COM9-StopBits": "2", "405 AO-Description": "NI DAC", "COM4-DelayBetweenCharsMs": "0.0000", "445 AO-Block voltage": "false", "520 AO-TriggerInputLine": "/Dev1/PFI0", "555 AO-Description": "NI DAC", "520 AO-TriggerSampleRate": "100000", "COM4-Verbose": "1", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM4-Name": "COM4", "405 Shutter-Volts": "0.0000", "Vincent-D3-Port": "COM1", "PRIME-PP 4 MIN ADU AFFECTED": "200", "470 Shutter-MinVolts": "0.0000", "PRIME-PP 3 ENABLED": "Yes", "470 AO-Description": "NI DAC", "PRIME-Description": "PVCAM API device adapter", "520 Shutter-MaxVolts": "5.0000", "520 Shutter-Volts": "0.0000", "528 AO-Sequenceable": "1", "PRIME-Binning": "1x1", "555 Shutter-MaxVolts": "5.0000", "528 Shutter-TriggerSequenceLength": "1000", "405 Shutter-Block voltage": "false", "XLIGHT Spin motor-State": "1", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "Vincent-D3-Time to open (ms)": "35.0000", "Vincent-D3-Time to close (ms)": "35.0000", "445 AO-TriggerSampleRate": "100000", "640 Shutter-IOChannel": "Dev1/ao14", "555 AO-Sequenceable": "1", "520 AO-Name": "AnalogIO", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "470 AO-Block voltage": "false", "COM1-Verbose": "1", "COM9-AnswerTimeout": "10000.0000", "528 Shutter-Name": "AnalogIO", "PRIME-Trigger Timeout (secs)": "10", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "NextFrame": 0, "470 AO-TriggerSequenceLength": "1000", "PRIME-TransposeXY": "0", "445 AO-TriggerSequenceLength": "1000", "COM1-Name": "COM1", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "COM9-DataBits": "8", "COM1-AnswerTimeout": "500.0000", "Camera": "", "TE2000-Version": "V2.50-V2.50-V2.10", "528 Shutter-Volts": "0.0000", "XLIGHT Hub-Name": "XLIGHT Hub", "PIEZO-Description": "Ludl stage driver adapter", "PositionName": "Pos34", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSampleRate": "100000", "PRIME-FanSpeedSetpoint": "High", "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "405 AO-TriggerSampleRate": "100000", "470 Shutter-Block voltage": "false", "470-State": "0", "COM3-Parity": "None", "405 AO-Volts": "0.0000", "PRIME-TransposeMirrorY": "0", "PRIME-ChipName": "GS144BSI", "470 AO-Volts": "0.0000", "PRIME-TransposeMirrorX": "0", "445 Shutter-SupportsTriggering": "0", "470 Shutter-SupportsTriggering": "0", "XLIGHT Hub-Description": "XLIGHT hub", "PRIME-PreampOffLimit": "10000", "470 AO-Sequenceable": "1", "528 Shutter-MaxVolts": "5.0000", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "Core-XYStage": "XYStage", "470 AO-TriggerSampleRate": "100000", "PRIME-Timing-PreTriggerDelayNs": "24672000.0000", "528-DA Device": "528 Shutter", "Focus-Description": "Nikon TE2000 focus adapter", "FilterBlock-State": "5", "520 AO-Demo": "No", "COM3-DelayBetweenCharsMs": "0.0000", "470 Shutter-Volts": "0.0000", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "PRIME-PP 2 THRESHOLD": "125", "PRIME-PP 4 THRESHOLD": "75", "XYStage-Acceleration": "4.0000", "470 Shutter-Demo": "No", "PRIME-Name": "PMPCIECam00", "640-DA Device": "640 Shutter", "405 AO-Block voltage": "false", "ElapsedTime-ms": 1420408, "PRIME-Offset": "100", "445 AO-IOChannel": "Dev1/ao1", "640 AO-MaxVolts": "5.0000", "PositionIndex": 34, "PRIME-PP 1 ENABLED": "Yes", "COM1-BaudRate": "9600", "640-State": "0", "COM3-Verbose": "1", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "LudlController-TransmissionDelay": "0", "PRIME-FullWellCapacity": "62000", "PRIME-SMARTStreamingEnabled": "No", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "640 Shutter-Description": "NI DAC", "528-Description": "DA device that is used as a shutter", "COM4-Description": "Serial port driver (boost:asio)", "405 Shutter-Description": "NI DAC", "TE2000-Name": "TE2000", "528 Shutter-Description": "NI DAC", "COM9-Description": "Serial port driver (boost:asio)", "LudlController-Name": "LudlController", "445 Shutter-TriggerSequenceLength": "1000", "PRIME-SerialNumber": "A18B203008", "445 AO-Volts": "0.0000", "PRIME-X-dimension": "1200", "PRIME-ShutterCloseDelay": "0", "XLIGHT Disk slider-State": "2", "Position": "Default", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 AO-IOChannel": "Dev1/ao5", "520 AO-IOChannel": "Dev1/ao4", "528 AO-TriggerSequenceLength": "1000", "405 Shutter-MaxVolts": "5.0000", "470-DA Device": "470 Shutter", "PRIME-FTCapable": "No", "Core-ChannelGroup": "Channels", "555 AO-SupportsTriggering": "0", "PRIME-Y-dimension": "1200", "Frame": 0, "555 Shutter-Sequenceable": "1", "PRIME-Timing-ExposureTimeNs": "499998000.0000", "Core-ImageProcessor": "", "528-State": "0", "520 AO-Block voltage": "false", "XLIGHT Excitation Wheel-Label": "Excitation-2", "640 Shutter-Demo": "No", "COM3-StopBits": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "520 Shutter-IOChannel": "Dev1/ao11", "Width": 1200, "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-SupportsTriggering": "0", "640 AO-TriggerSampleRate": "100000", "520-Description": "DA device that is used as a shutter", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "405 Shutter-Sequenceable": "1", "405 Shutter-SupportsTriggering": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-TriggerSampleRate": "100000", "640 Shutter-MaxVolts": "5.0000", "555 AO-MaxVolts": "5.0000", "520 AO-TriggerSequenceLength": "1000", "COM3-BaudRate": "9600", "COM9-BaudRate": "9600", "Vincent-D3-Channel #": "Ch. #1", "PRIME-PMode": "Normal", "OpticalPath-Label": "Left(100)", "BitDepth": 16, "555 AO-TriggerSequenceLength": "1000", "445 Shutter-Description": "NI DAC", "405 AO-Sequenceable": "1", "PRIME-AcquisitionMethod": "Callbacks", "555 AO-Name": "AnalogIO", "PRIME-ShutterMode": "Pre-Exposure", "COM3-Handshaking": "Off", "445 AO-SupportsTriggering": "0", "ROI": "0-0-1200-1200", "COM9-Verbose": "1", "PRIME-Color": "OFF", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "PRIME-ShutterOpenDelay": "0", "COM1-Parity": "None", "Slice": 5, "SlicePosition": 0, "COM9-DelayBetweenCharsMs": "0.0000", "555-Name": "DA Shutter", "PIEZO-LudlSingleAxisName": "B", "XLIGHT Emission Wheel-Label": "Empty", "640 Shutter-Name": "AnalogIO", "640 AO-Sequenceable": "1", "520 Shutter-Demo": "No", "528 Shutter-Demo": "No", "470-Name": "DA Shutter", "520-DA Device": "520 Shutter", "XYStage-Description": "Ludl XY stage driver adapter", "445 Shutter-MinVolts": "0.0000", "528 AO-TriggerSampleRate": "100000", "405 AO-MinVolts": "0.0000", "405 Shutter-TriggerSequenceLength": "1000", "Height": 1200, "555 AO-Block voltage": "false", "640 Shutter-Volts": "0.0000", "PixelType": "GRAY16", "445 Shutter-Block voltage": "false", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "520 Shutter-TriggerSequenceLength": "1000", "COM9-Name": "COM9", "Core-SLM": "", "555 AO-Volts": "0.0000", "555 Shutter-Block voltage": "false", "XYStage-Speed": "1800.0000", "520 AO-MaxVolts": "5.0000", "528 Shutter-SupportsTriggering": "0", "COM1-Description": "Serial port driver (boost:asio)", "PRIME-MetadataEnabled": "No", "520 Shutter-MinVolts": "0.0000", "470 Shutter-Sequenceable": "1", "PRIME-PreampDelay": "0", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-MinVolts": "0.0000", "ChannelIndex": 4, "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Core-AutoShutter": "1", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "Exposure-ms": 20, "PRIME-PP 2 ENABLED": "Yes", "640 Shutter-TriggerSampleRate": "100000", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "470 Shutter-IOChannel": "Dev1/ao10", "405 AO-Demo": "No", "PixelSizeUm": 1.1, "470 AO-Demo": "No", "470 Shutter-MaxVolts": "5.0000", "Vincent-D3-Name": "Vincent-D3", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FileName": "img_000000000_P-DIC_005.tif", "LudlController-Reset": "Operate", "PRIME-Exposure": "20.00", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "445-Name": "DA Shutter", "555 Shutter-Name": "AnalogIO", "520 Shutter-TriggerSampleRate": "100000", "445 Shutter-IOChannel": "Dev1/ao9", "PRIME-TransposeCorrection": "0", "XYStage-StepSize": "0.1000", "445-Description": "DA device that is used as a shutter", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 AO-IOChannel": "Dev1/ao7", "520 Shutter-Name": "AnalogIO", "COM3-DataBits": "8", "640 AO-Volts": "0.0000", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "555 Shutter-IOChannel": "Dev1/ao13", "WaitInterval": null, "520 Shutter-Sequenceable": "1", "YPositionUm": 545.6, "445-State": "0", "445-DA Device": "445 Shutter", "PRIME-CircularBufferAutoSize": "ON", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "555 AO-TriggerSampleRate": "100000", "445 Shutter-Volts": "0.0000", "XLIGHT Hub-Port": "COM3", "Core-AutoFocus": "", "COM4-Handshaking": "Off", "528 Shutter-MinVolts": "0.0000", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "445 Shutter-Name": "AnalogIO", "528 Shutter-Sequenceable": "1", "XYStage-TransposeMirrorX": "0", "XYStage-TransposeMirrorY": "0", "PRIME-ReadoutRate": "100MHz 16bit", "XYStage-Name": "Stage", "640 AO-SupportsTriggering": "0", "640-Name": "DA Shutter", "640 Shutter-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "640 AO-Block voltage": "false", "445 Shutter-Sequenceable": "1", "470 AO-MaxVolts": "5.0000", "COM4-BaudRate": "9600", "470-Description": "DA device that is used as a shutter", "520 Shutter-Description": "NI DAC", "PRIME-CCDTemperature": "-14.8500", "Time": "2019-12-19 09:59:50 -0500", "640 AO-TriggerSequenceLength": "1000", "470 AO-Name": "AnalogIO", "XLIGHT Disk slider-Label": "Disk Pos 40um", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "Binning": "1x1", "TE2000-ModelType": "1", "COM3-AnswerTimeout": "500.0000", "555 Shutter-SupportsTriggering": "0", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "TE2000-Port": "COM4", "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Description": "NI DAC", "COM4-AnswerTimeout": "500.0000", "COM1-DelayBetweenCharsMs": "0.0000", "FilterBlock-Label": "Confocal", "445 AO-Name": "AnalogIO", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-Timing-ReadoutTimeNs": "24672000.0000", "528 AO-Demo": "No", "520 AO-Sequenceable": "1", "555-Description": "DA device that is used as a shutter", "405-DA Device": "405 Shutter", "405 AO-TriggerSequenceLength": "1000", "640 AO-MinVolts": "0.0000", "640 Shutter-Block voltage": "false", "FrameIndex": 0, "PRIME-Port": "CMOS", "555 AO-MinVolts": "0.0000", "COM3-Description": "Serial port driver (boost:asio)", "528 AO-Description": "NI DAC", "555 AO-IOChannel": "Dev1/ao6", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "555 AO-TriggerInputLine": "/Dev1/PFI0", "PRIME-ActualInterval-ms": "390.5200", "LudlController-Port": "COM9", "445 Shutter-Demo": "No", "PRIME-Trigger-Expose Out-Mux": "1", "SliceIndex": 5, "PRIME-CCDTemperatureSetPoint": "-15.0000", "405 Shutter-MinVolts": "0.0000", "PRIME-ExposeOutMode": "First Row", "AxisPositions": null, "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Volts": "0.0000", "528-Name": "DA Shutter", "555-State": "0", "XLIGHT Dichroic-Label": "Di03-R635-t1", "445 AO-Sequenceable": "1", "405-Name": "DA Shutter", "405 AO-IOChannel": "Dev1/ao0", "Core-Camera": "PRIME", "Focus-Name": "Focus", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "405 Shutter-IOChannel": "Dev1/ao8", "640 AO-Demo": "No", "640 Shutter-SupportsTriggering": "0", "445 AO-Demo": "No", "555-DA Device": "555 Shutter", "470 Shutter-Description": "NI DAC", "528 AO-Volts": "0.0000", "COM1-DataBits": "8", "Vincent-D3-Address": "x", "520 AO-SupportsTriggering": "0", "405-Description": "DA device that is used as a shutter", "640 Shutter-MinVolts": "0.0000", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "405 AO-Name": "AnalogIO", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "640 AO-TriggerInputLine": "/Dev1/PFI0", "405 AO-TriggerInputLine": "/Dev1/PFI0", "640-Description": "DA device that is used as a shutter", "445 Shutter-TriggerSampleRate": "100000", "470 Shutter-TriggerSequenceLength": "1000", "COM1-StopBits": "1", "520 Shutter-SupportsTriggering": "0", "405-State": "0", "Core-Initialize": "1", "XLIGHT Emission Wheel-State": "3", "520 AO-Description": "NI DAC", "528 AO-TriggerInputLine": "/Dev1/PFI0", "445 Shutter-MaxVolts": "5.0000", "COM9-Handshaking": "Off", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "XLIGHT Spin motor-Label": "On (spinning)", "PRIME-PP 5 ENABLED": "No", "UUID": "cd7e4150-1897-4ee6-9be4-7c64a86970be", "PRIME-TriggerMode": "Internal Trigger", "COM1-Handshaking": "Off", "PRIME-Timing-PostTriggerDelayNs": "49344000.0000", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "Focus-Position": "17741.5000", "528 Shutter-Block voltage": "false", "Channel": "P-DIC", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "555 Shutter-Demo": "No", "405 Shutter-Demo": "No", "528 AO-Name": "AnalogIO", "520-State": "0", "Core-Galvo": "", "405 Shutter-TriggerSampleRate": "100000", "Focus-FocusStepSize_(nm)": "100", "PRIME-PixelType": "16bit", "COM4-DataBits": "8", "470 AO-IOChannel": "Dev1/ao2", "520 AO-MinVolts": "0.0000", "COM9-Parity": "None", "PRIME-CircularBufferEnabled": "ON", "PRIME-PP 6 Reset": "No", "640 AO-Description": "NI DAC", "Source": "PRIME", "445 AO-MinVolts": "0.0000", "PIEZO-Name": "Stage", "445 AO-Description": "NI DAC", "PRIME-CircularBufferFrameCount": "46", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "520 AO-Volts": "0.0000", "528 Shutter-TriggerSampleRate": "100000", "XPositionUm": -1324.9, "OpticalPath-State": "4", "PRIME-PP 3 THRESHOLD": "80", "PRIME-PVCAM Version": "3.7.1", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Gain": "1-HDR", "640 Shutter-TriggerSequenceLength": "1000", "528 AO-SupportsTriggering": "0", "445 AO-TriggerInputLine": "/Dev1/PFI0", "OpticalPath-Name": "OpticalPath", "PRIME-PP 4 ENABLED": "Yes", "PRIME-PVCAM Adapter Version": "1.3.29", "COM3-Name": "COM3", "405 AO-MaxVolts": "5.0000", "520 Shutter-Block voltage": "false", "PRIME-Timing-ClearingTimeNs": "49344000.0000", "Core-Focus": "PIEZO", "528 AO-Block voltage": "false", "TE2000-Description": "Nikon TE2000 hub", "528 AO-MinVolts": "0.0000", "COM4-Parity": "None", "Summary": { "Slices": 11, "ChContrastMin": [ 0, 0, 0, 0, 0, 0 ], "PixelAspect": 1, "SlicesFirst": true, "Interval_ms": 0, "Time": "2019-12-19 09:59:21 -0500", "Prefix": "seq_13", "InitialPositionList": [ { "Label": "Pos0", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.21], "Focus": [17741.5], "XYStage": [ 44542.200000000004, 31636.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos1", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.22], "Focus": [17741.4], "XYStage": [ 43801.100000000006, 31637.9 ] }, "GridColumnIndex": 0 }, { "Label": "Pos2", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.5], "XYStage": [ 38561.3, 32040.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos3", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.185], "Focus": [17741.3], "XYStage": [ 37698.4, 32041.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos4", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.16], "Focus": [17741.5], "XYStage": [ 32375.4, 31758.9 ] }, "GridColumnIndex": 0 }, { "Label": "Pos5", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.17000000000002], "Focus": [17741.3], "XYStage": [ 31770.7, 31758.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos6", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.4], "XYStage": [ 26536.800000000003, 31068.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos7", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.205], "Focus": [17741.4], "XYStage": [ 25420.2, 31068.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos8", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.85], "Focus": [17741.3], "XYStage": [ 21059, 31508 ] }, "GridColumnIndex": 0 }, { "Label": "Pos9", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.82], "Focus": [17741.3], "XYStage": [ 19731.9, 31506.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos10", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.79], "Focus": [17741.4], "XYStage": [ 14528.5, 32007.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos11", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.83], "Focus": [17741.2], "XYStage": [ 13830.300000000001, 32009.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos12", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.79], "Focus": [17741.3], "XYStage": [ 8775.300000000001, 32017.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos13", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.815], "Focus": [17741.3], "XYStage": [ 8109.200000000001, 32008.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos14", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.805], "Focus": [17741.3], "XYStage": [ 3483.3, 31908.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos15", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.82], "Focus": [17741.2], "XYStage": [ 2686.8, 31903.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos16", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [211.48000000000002], "Focus": [17741.1], "XYStage": [ -1978.9, 31973.600000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos17", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [211.51500000000001], "Focus": [17741.1], "XYStage": [ -2957.8, 31982.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos18", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.195], "Focus": [17741.5], "XYStage": [ 47747.4, -576.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos19", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.23000000000002], "Focus": [17741.6], "XYStage": [ 47053.9, -576.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos20", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.205], "Focus": [17741.4], "XYStage": [ 41871.200000000004, -479.8 ] }, "GridColumnIndex": 0 }, { "Label": "Pos21", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.4], "XYStage": [ 40882.600000000006, -392 ] }, "GridColumnIndex": 0 }, { "Label": "Pos22", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.24], "Focus": [17741.4], "XYStage": [ 35905.6, -135.3 ] }, "GridColumnIndex": 0 }, { "Label": "Pos23", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.195], "Focus": [17741.5], "XYStage": [ 34990.9, -132.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos24", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.46], "Focus": [17741.4], "XYStage": [ 29678.100000000002, -958.1 ] }, "GridColumnIndex": 0 }, { "Label": "Pos25", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.44], "Focus": [17741.5], "XYStage": [ 28983.600000000002, -939.4000000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos26", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.475], "Focus": [17741.5], "XYStage": [ 23019.7, -122 ] }, "GridColumnIndex": 0 }, { "Label": "Pos27", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.44], "Focus": [17741.7], "XYStage": [ 22428, -116.80000000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos28", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.46], "Focus": [17741.7], "XYStage": [ 16364.900000000001, -181.10000000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos29", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.48000000000002], "Focus": [17741.5], "XYStage": [ 15712.1, -180 ] }, "GridColumnIndex": 0 }, { "Label": "Pos30", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.45000000000002], "Focus": [17741.7], "XYStage": [ 10346.1, 77.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos31", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.49], "Focus": [17741.4], "XYStage": [ 9755.5, 85.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos32", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.475], "Focus": [17741.5], "XYStage": [ 4775.5, 293.90000000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos33", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.445], "Focus": [17741.4], "XYStage": [ 3954.9, 293.90000000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos34", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.455], "Focus": [17741.4], "XYStage": [ -1324.9, 545.6 ] }, "GridColumnIndex": 0 }, { "Label": "Pos35", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.445], "Focus": [17741.3], "XYStage": [ -2097, 576.6 ] }, "GridColumnIndex": 0 } ], "z-step_um": 10, "GridRow": 0, "BitDepth": 16, "Depth": 2, "Channels": 6, "Source": "Micro-Manager", "ChColors": [ -16711681, -256, -65281, -1, -1, -16776961 ], "MetadataVersion": 10, "KeepShutterOpenChannels": true, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536, 65536 ], "CustomIntervals_ms": [], "Height": 1200, "MicroManagerVersion": "1.4.23 20180219", "GridColumn": 0, "UUID": "18808e7c-f2e6-47b3-90d8-1d779ce87dbd", "PixelType": "GRAY16", "Width": 1200, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\Yan\\20191218-YC113-S7S8-miseq", "Frames": 1, "Comment": "", "UserName": "Nikon", "PixelSize_um": 1.1, "ROI": [ 0, 0, 1200, 1200 ], "Date": "2019-12-19", "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC", "P-DAPI-high" ], "KeepShutterOpenSlices": true, "IJType": 1, "ComputerName": "ZadorConfocal2", "Positions": 36, "PositionIndex": 34, "CameraTimeout": 20000, "TimeFirst": false }, "640 AO-Name": "AnalogIO", "Vincent-D3-Command": "Close" }, "FrameKey-0-4-6": { "COM4-StopBits": "1", "528 AO-MaxVolts": "5.0000", "XLIGHT Excitation Wheel-State": "1", "470 Shutter-Name": "AnalogIO", "Core-Shutter": "Vincent-D3", "555 AO-Demo": "No", "XLIGHT Dichroic-State": "2", "XYStage-StepSize-Y": "0.1000", "ZPositionUm": 228.455, "XYStage-StepSize-X": "0.1000", "PRIME-FirmwareVersion": "20.28", "PRIME-ClearCycles": "2", "520-Name": "DA Shutter", "405 Shutter-Name": "AnalogIO", "PIEZO-StepSize": "0.0050", "PRIME-ClearMode": "Pre-Exposure", "470 AO-MinVolts": "0.0000", "405 AO-SupportsTriggering": "1", "CameraChannelIndex": 0, "XYStage-StartSpeed": "500.0000", "COM9-StopBits": "2", "405 AO-Description": "NI DAC", "COM4-DelayBetweenCharsMs": "0.0000", "445 AO-Block voltage": "false", "520 AO-TriggerInputLine": "/Dev1/PFI0", "555 AO-Description": "NI DAC", "520 AO-TriggerSampleRate": "100000", "COM4-Verbose": "1", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM4-Name": "COM4", "405 Shutter-Volts": "0.0000", "Vincent-D3-Port": "COM1", "PRIME-PP 4 MIN ADU AFFECTED": "200", "470 Shutter-MinVolts": "0.0000", "PRIME-PP 3 ENABLED": "Yes", "470 AO-Description": "NI DAC", "PRIME-Description": "PVCAM API device adapter", "520 Shutter-MaxVolts": "5.0000", "520 Shutter-Volts": "0.0000", "528 AO-Sequenceable": "1", "PRIME-Binning": "1x1", "555 Shutter-MaxVolts": "5.0000", "528 Shutter-TriggerSequenceLength": "1000", "405 Shutter-Block voltage": "false", "XLIGHT Spin motor-State": "1", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "Vincent-D3-Time to open (ms)": "35.0000", "Vincent-D3-Time to close (ms)": "35.0000", "445 AO-TriggerSampleRate": "100000", "640 Shutter-IOChannel": "Dev1/ao14", "555 AO-Sequenceable": "1", "520 AO-Name": "AnalogIO", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "470 AO-Block voltage": "false", "COM1-Verbose": "1", "COM9-AnswerTimeout": "10000.0000", "528 Shutter-Name": "AnalogIO", "PRIME-Trigger Timeout (secs)": "10", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "NextFrame": 0, "470 AO-TriggerSequenceLength": "1000", "PRIME-TransposeXY": "0", "445 AO-TriggerSequenceLength": "1000", "COM1-Name": "COM1", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "COM9-DataBits": "8", "COM1-AnswerTimeout": "500.0000", "Camera": "", "TE2000-Version": "V2.50-V2.50-V2.10", "528 Shutter-Volts": "0.0000", "XLIGHT Hub-Name": "XLIGHT Hub", "PIEZO-Description": "Ludl stage driver adapter", "PositionName": "Pos34", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSampleRate": "100000", "PRIME-FanSpeedSetpoint": "High", "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "405 AO-TriggerSampleRate": "100000", "470 Shutter-Block voltage": "false", "470-State": "0", "COM3-Parity": "None", "405 AO-Volts": "0.0000", "PRIME-TransposeMirrorY": "0", "PRIME-ChipName": "GS144BSI", "470 AO-Volts": "0.0000", "PRIME-TransposeMirrorX": "0", "445 Shutter-SupportsTriggering": "0", "470 Shutter-SupportsTriggering": "0", "XLIGHT Hub-Description": "XLIGHT hub", "PRIME-PreampOffLimit": "10000", "470 AO-Sequenceable": "1", "528 Shutter-MaxVolts": "5.0000", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "Core-XYStage": "XYStage", "470 AO-TriggerSampleRate": "100000", "PRIME-Timing-PreTriggerDelayNs": "24672000.0000", "528-DA Device": "528 Shutter", "Focus-Description": "Nikon TE2000 focus adapter", "FilterBlock-State": "5", "520 AO-Demo": "No", "COM3-DelayBetweenCharsMs": "0.0000", "470 Shutter-Volts": "0.0000", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "PRIME-PP 2 THRESHOLD": "125", "PRIME-PP 4 THRESHOLD": "75", "XYStage-Acceleration": "4.0000", "470 Shutter-Demo": "No", "PRIME-Name": "PMPCIECam00", "640-DA Device": "640 Shutter", "405 AO-Block voltage": "false", "ElapsedTime-ms": 1420663, "PRIME-Offset": "100", "445 AO-IOChannel": "Dev1/ao1", "640 AO-MaxVolts": "5.0000", "PositionIndex": 34, "PRIME-PP 1 ENABLED": "Yes", "COM1-BaudRate": "9600", "640-State": "0", "COM3-Verbose": "1", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "LudlController-TransmissionDelay": "0", "PRIME-FullWellCapacity": "62000", "PRIME-SMARTStreamingEnabled": "No", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "640 Shutter-Description": "NI DAC", "528-Description": "DA device that is used as a shutter", "COM4-Description": "Serial port driver (boost:asio)", "405 Shutter-Description": "NI DAC", "TE2000-Name": "TE2000", "528 Shutter-Description": "NI DAC", "COM9-Description": "Serial port driver (boost:asio)", "LudlController-Name": "LudlController", "445 Shutter-TriggerSequenceLength": "1000", "PRIME-SerialNumber": "A18B203008", "445 AO-Volts": "0.0000", "PRIME-X-dimension": "1200", "PRIME-ShutterCloseDelay": "0", "XLIGHT Disk slider-State": "2", "Position": "Default", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 AO-IOChannel": "Dev1/ao5", "520 AO-IOChannel": "Dev1/ao4", "528 AO-TriggerSequenceLength": "1000", "405 Shutter-MaxVolts": "5.0000", "470-DA Device": "470 Shutter", "PRIME-FTCapable": "No", "Core-ChannelGroup": "Channels", "555 AO-SupportsTriggering": "0", "PRIME-Y-dimension": "1200", "Frame": 0, "555 Shutter-Sequenceable": "1", "PRIME-Timing-ExposureTimeNs": "499998000.0000", "Core-ImageProcessor": "", "528-State": "0", "520 AO-Block voltage": "false", "XLIGHT Excitation Wheel-Label": "Excitation-2", "640 Shutter-Demo": "No", "COM3-StopBits": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "520 Shutter-IOChannel": "Dev1/ao11", "Width": 1200, "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-SupportsTriggering": "0", "640 AO-TriggerSampleRate": "100000", "520-Description": "DA device that is used as a shutter", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "405 Shutter-Sequenceable": "1", "405 Shutter-SupportsTriggering": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-TriggerSampleRate": "100000", "640 Shutter-MaxVolts": "5.0000", "555 AO-MaxVolts": "5.0000", "520 AO-TriggerSequenceLength": "1000", "COM3-BaudRate": "9600", "COM9-BaudRate": "9600", "Vincent-D3-Channel #": "Ch. #1", "PRIME-PMode": "Normal", "OpticalPath-Label": "Left(100)", "BitDepth": 16, "555 AO-TriggerSequenceLength": "1000", "445 Shutter-Description": "NI DAC", "405 AO-Sequenceable": "1", "PRIME-AcquisitionMethod": "Callbacks", "555 AO-Name": "AnalogIO", "PRIME-ShutterMode": "Pre-Exposure", "COM3-Handshaking": "Off", "445 AO-SupportsTriggering": "0", "ROI": "0-0-1200-1200", "COM9-Verbose": "1", "PRIME-Color": "OFF", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "PRIME-ShutterOpenDelay": "0", "COM1-Parity": "None", "Slice": 6, "SlicePosition": 10, "COM9-DelayBetweenCharsMs": "0.0000", "555-Name": "DA Shutter", "PIEZO-LudlSingleAxisName": "B", "XLIGHT Emission Wheel-Label": "Empty", "640 Shutter-Name": "AnalogIO", "640 AO-Sequenceable": "1", "520 Shutter-Demo": "No", "528 Shutter-Demo": "No", "470-Name": "DA Shutter", "520-DA Device": "520 Shutter", "XYStage-Description": "Ludl XY stage driver adapter", "445 Shutter-MinVolts": "0.0000", "528 AO-TriggerSampleRate": "100000", "405 AO-MinVolts": "0.0000", "405 Shutter-TriggerSequenceLength": "1000", "Height": 1200, "555 AO-Block voltage": "false", "640 Shutter-Volts": "0.0000", "PixelType": "GRAY16", "445 Shutter-Block voltage": "false", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "520 Shutter-TriggerSequenceLength": "1000", "COM9-Name": "COM9", "Core-SLM": "", "555 AO-Volts": "0.0000", "555 Shutter-Block voltage": "false", "XYStage-Speed": "1800.0000", "520 AO-MaxVolts": "5.0000", "528 Shutter-SupportsTriggering": "0", "COM1-Description": "Serial port driver (boost:asio)", "PRIME-MetadataEnabled": "No", "520 Shutter-MinVolts": "0.0000", "470 Shutter-Sequenceable": "1", "PRIME-PreampDelay": "0", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-MinVolts": "0.0000", "ChannelIndex": 4, "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Core-AutoShutter": "1", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "Exposure-ms": 20, "PRIME-PP 2 ENABLED": "Yes", "640 Shutter-TriggerSampleRate": "100000", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "470 Shutter-IOChannel": "Dev1/ao10", "405 AO-Demo": "No", "PixelSizeUm": 1.1, "470 AO-Demo": "No", "470 Shutter-MaxVolts": "5.0000", "Vincent-D3-Name": "Vincent-D3", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FileName": "img_000000000_P-DIC_006.tif", "LudlController-Reset": "Operate", "PRIME-Exposure": "20.00", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "445-Name": "DA Shutter", "555 Shutter-Name": "AnalogIO", "520 Shutter-TriggerSampleRate": "100000", "445 Shutter-IOChannel": "Dev1/ao9", "PRIME-TransposeCorrection": "0", "XYStage-StepSize": "0.1000", "445-Description": "DA device that is used as a shutter", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 AO-IOChannel": "Dev1/ao7", "520 Shutter-Name": "AnalogIO", "COM3-DataBits": "8", "640 AO-Volts": "0.0000", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "555 Shutter-IOChannel": "Dev1/ao13", "WaitInterval": null, "520 Shutter-Sequenceable": "1", "YPositionUm": 545.6, "445-State": "0", "445-DA Device": "445 Shutter", "PRIME-CircularBufferAutoSize": "ON", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "555 AO-TriggerSampleRate": "100000", "445 Shutter-Volts": "0.0000", "XLIGHT Hub-Port": "COM3", "Core-AutoFocus": "", "COM4-Handshaking": "Off", "528 Shutter-MinVolts": "0.0000", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "445 Shutter-Name": "AnalogIO", "528 Shutter-Sequenceable": "1", "XYStage-TransposeMirrorX": "0", "XYStage-TransposeMirrorY": "0", "PRIME-ReadoutRate": "100MHz 16bit", "XYStage-Name": "Stage", "640 AO-SupportsTriggering": "0", "640-Name": "DA Shutter", "640 Shutter-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "640 AO-Block voltage": "false", "445 Shutter-Sequenceable": "1", "470 AO-MaxVolts": "5.0000", "COM4-BaudRate": "9600", "470-Description": "DA device that is used as a shutter", "520 Shutter-Description": "NI DAC", "PRIME-CCDTemperature": "-14.8500", "Time": "2019-12-19 09:59:51 -0500", "640 AO-TriggerSequenceLength": "1000", "470 AO-Name": "AnalogIO", "XLIGHT Disk slider-Label": "Disk Pos 40um", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "Binning": "1x1", "TE2000-ModelType": "1", "COM3-AnswerTimeout": "500.0000", "555 Shutter-SupportsTriggering": "0", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "TE2000-Port": "COM4", "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Description": "NI DAC", "COM4-AnswerTimeout": "500.0000", "COM1-DelayBetweenCharsMs": "0.0000", "FilterBlock-Label": "Confocal", "445 AO-Name": "AnalogIO", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-Timing-ReadoutTimeNs": "24672000.0000", "528 AO-Demo": "No", "520 AO-Sequenceable": "1", "555-Description": "DA device that is used as a shutter", "405-DA Device": "405 Shutter", "405 AO-TriggerSequenceLength": "1000", "640 AO-MinVolts": "0.0000", "640 Shutter-Block voltage": "false", "FrameIndex": 0, "PRIME-Port": "CMOS", "555 AO-MinVolts": "0.0000", "COM3-Description": "Serial port driver (boost:asio)", "528 AO-Description": "NI DAC", "555 AO-IOChannel": "Dev1/ao6", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "555 AO-TriggerInputLine": "/Dev1/PFI0", "PRIME-ActualInterval-ms": "390.5200", "LudlController-Port": "COM9", "445 Shutter-Demo": "No", "PRIME-Trigger-Expose Out-Mux": "1", "SliceIndex": 6, "PRIME-CCDTemperatureSetPoint": "-15.0000", "405 Shutter-MinVolts": "0.0000", "PRIME-ExposeOutMode": "First Row", "AxisPositions": null, "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Volts": "0.0000", "528-Name": "DA Shutter", "555-State": "0", "XLIGHT Dichroic-Label": "Di03-R635-t1", "445 AO-Sequenceable": "1", "405-Name": "DA Shutter", "405 AO-IOChannel": "Dev1/ao0", "Core-Camera": "PRIME", "Focus-Name": "Focus", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "405 Shutter-IOChannel": "Dev1/ao8", "640 AO-Demo": "No", "640 Shutter-SupportsTriggering": "0", "445 AO-Demo": "No", "555-DA Device": "555 Shutter", "470 Shutter-Description": "NI DAC", "528 AO-Volts": "0.0000", "COM1-DataBits": "8", "Vincent-D3-Address": "x", "520 AO-SupportsTriggering": "0", "405-Description": "DA device that is used as a shutter", "640 Shutter-MinVolts": "0.0000", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "405 AO-Name": "AnalogIO", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "640 AO-TriggerInputLine": "/Dev1/PFI0", "405 AO-TriggerInputLine": "/Dev1/PFI0", "640-Description": "DA device that is used as a shutter", "445 Shutter-TriggerSampleRate": "100000", "470 Shutter-TriggerSequenceLength": "1000", "COM1-StopBits": "1", "520 Shutter-SupportsTriggering": "0", "405-State": "0", "Core-Initialize": "1", "XLIGHT Emission Wheel-State": "3", "520 AO-Description": "NI DAC", "528 AO-TriggerInputLine": "/Dev1/PFI0", "445 Shutter-MaxVolts": "5.0000", "COM9-Handshaking": "Off", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "XLIGHT Spin motor-Label": "On (spinning)", "PRIME-PP 5 ENABLED": "No", "UUID": "ee125ea9-caa4-4b70-a30d-f0d69c7964b9", "PRIME-TriggerMode": "Internal Trigger", "COM1-Handshaking": "Off", "PRIME-Timing-PostTriggerDelayNs": "49344000.0000", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "Focus-Position": "17741.5000", "528 Shutter-Block voltage": "false", "Channel": "P-DIC", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "555 Shutter-Demo": "No", "405 Shutter-Demo": "No", "528 AO-Name": "AnalogIO", "520-State": "0", "Core-Galvo": "", "405 Shutter-TriggerSampleRate": "100000", "Focus-FocusStepSize_(nm)": "100", "PRIME-PixelType": "16bit", "COM4-DataBits": "8", "470 AO-IOChannel": "Dev1/ao2", "520 AO-MinVolts": "0.0000", "COM9-Parity": "None", "PRIME-CircularBufferEnabled": "ON", "PRIME-PP 6 Reset": "No", "640 AO-Description": "NI DAC", "Source": "PRIME", "445 AO-MinVolts": "0.0000", "PIEZO-Name": "Stage", "445 AO-Description": "NI DAC", "PRIME-CircularBufferFrameCount": "46", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "520 AO-Volts": "0.0000", "528 Shutter-TriggerSampleRate": "100000", "XPositionUm": -1324.9, "OpticalPath-State": "4", "PRIME-PP 3 THRESHOLD": "80", "PRIME-PVCAM Version": "3.7.1", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Gain": "1-HDR", "640 Shutter-TriggerSequenceLength": "1000", "528 AO-SupportsTriggering": "0", "445 AO-TriggerInputLine": "/Dev1/PFI0", "OpticalPath-Name": "OpticalPath", "PRIME-PP 4 ENABLED": "Yes", "PRIME-PVCAM Adapter Version": "1.3.29", "COM3-Name": "COM3", "405 AO-MaxVolts": "5.0000", "520 Shutter-Block voltage": "false", "PRIME-Timing-ClearingTimeNs": "49344000.0000", "Core-Focus": "PIEZO", "528 AO-Block voltage": "false", "TE2000-Description": "Nikon TE2000 hub", "528 AO-MinVolts": "0.0000", "COM4-Parity": "None", "Summary": { "Slices": 11, "ChContrastMin": [ 0, 0, 0, 0, 0, 0 ], "PixelAspect": 1, "SlicesFirst": true, "Interval_ms": 0, "Time": "2019-12-19 09:59:21 -0500", "Prefix": "seq_13", "InitialPositionList": [ { "Label": "Pos0", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.21], "Focus": [17741.5], "XYStage": [ 44542.200000000004, 31636.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos1", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.22], "Focus": [17741.4], "XYStage": [ 43801.100000000006, 31637.9 ] }, "GridColumnIndex": 0 }, { "Label": "Pos2", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.5], "XYStage": [ 38561.3, 32040.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos3", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.185], "Focus": [17741.3], "XYStage": [ 37698.4, 32041.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos4", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.16], "Focus": [17741.5], "XYStage": [ 32375.4, 31758.9 ] }, "GridColumnIndex": 0 }, { "Label": "Pos5", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.17000000000002], "Focus": [17741.3], "XYStage": [ 31770.7, 31758.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos6", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.4], "XYStage": [ 26536.800000000003, 31068.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos7", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.205], "Focus": [17741.4], "XYStage": [ 25420.2, 31068.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos8", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.85], "Focus": [17741.3], "XYStage": [ 21059, 31508 ] }, "GridColumnIndex": 0 }, { "Label": "Pos9", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.82], "Focus": [17741.3], "XYStage": [ 19731.9, 31506.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos10", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.79], "Focus": [17741.4], "XYStage": [ 14528.5, 32007.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos11", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.83], "Focus": [17741.2], "XYStage": [ 13830.300000000001, 32009.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos12", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.79], "Focus": [17741.3], "XYStage": [ 8775.300000000001, 32017.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos13", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.815], "Focus": [17741.3], "XYStage": [ 8109.200000000001, 32008.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos14", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.805], "Focus": [17741.3], "XYStage": [ 3483.3, 31908.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos15", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.82], "Focus": [17741.2], "XYStage": [ 2686.8, 31903.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos16", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [211.48000000000002], "Focus": [17741.1], "XYStage": [ -1978.9, 31973.600000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos17", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [211.51500000000001], "Focus": [17741.1], "XYStage": [ -2957.8, 31982.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos18", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.195], "Focus": [17741.5], "XYStage": [ 47747.4, -576.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos19", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.23000000000002], "Focus": [17741.6], "XYStage": [ 47053.9, -576.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos20", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.205], "Focus": [17741.4], "XYStage": [ 41871.200000000004, -479.8 ] }, "GridColumnIndex": 0 }, { "Label": "Pos21", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.4], "XYStage": [ 40882.600000000006, -392 ] }, "GridColumnIndex": 0 }, { "Label": "Pos22", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.24], "Focus": [17741.4], "XYStage": [ 35905.6, -135.3 ] }, "GridColumnIndex": 0 }, { "Label": "Pos23", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.195], "Focus": [17741.5], "XYStage": [ 34990.9, -132.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos24", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.46], "Focus": [17741.4], "XYStage": [ 29678.100000000002, -958.1 ] }, "GridColumnIndex": 0 }, { "Label": "Pos25", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.44], "Focus": [17741.5], "XYStage": [ 28983.600000000002, -939.4000000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos26", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.475], "Focus": [17741.5], "XYStage": [ 23019.7, -122 ] }, "GridColumnIndex": 0 }, { "Label": "Pos27", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.44], "Focus": [17741.7], "XYStage": [ 22428, -116.80000000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos28", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.46], "Focus": [17741.7], "XYStage": [ 16364.900000000001, -181.10000000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos29", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.48000000000002], "Focus": [17741.5], "XYStage": [ 15712.1, -180 ] }, "GridColumnIndex": 0 }, { "Label": "Pos30", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.45000000000002], "Focus": [17741.7], "XYStage": [ 10346.1, 77.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos31", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.49], "Focus": [17741.4], "XYStage": [ 9755.5, 85.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos32", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.475], "Focus": [17741.5], "XYStage": [ 4775.5, 293.90000000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos33", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.445], "Focus": [17741.4], "XYStage": [ 3954.9, 293.90000000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos34", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.455], "Focus": [17741.4], "XYStage": [ -1324.9, 545.6 ] }, "GridColumnIndex": 0 }, { "Label": "Pos35", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.445], "Focus": [17741.3], "XYStage": [ -2097, 576.6 ] }, "GridColumnIndex": 0 } ], "z-step_um": 10, "GridRow": 0, "BitDepth": 16, "Depth": 2, "Channels": 6, "Source": "Micro-Manager", "ChColors": [ -16711681, -256, -65281, -1, -1, -16776961 ], "MetadataVersion": 10, "KeepShutterOpenChannels": true, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536, 65536 ], "CustomIntervals_ms": [], "Height": 1200, "MicroManagerVersion": "1.4.23 20180219", "GridColumn": 0, "UUID": "18808e7c-f2e6-47b3-90d8-1d779ce87dbd", "PixelType": "GRAY16", "Width": 1200, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\Yan\\20191218-YC113-S7S8-miseq", "Frames": 1, "Comment": "", "UserName": "Nikon", "PixelSize_um": 1.1, "ROI": [ 0, 0, 1200, 1200 ], "Date": "2019-12-19", "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC", "P-DAPI-high" ], "KeepShutterOpenSlices": true, "IJType": 1, "ComputerName": "ZadorConfocal2", "Positions": 36, "PositionIndex": 34, "CameraTimeout": 20000, "TimeFirst": false }, "640 AO-Name": "AnalogIO", "Vincent-D3-Command": "Close" }, "FrameKey-0-4-7": { "COM4-StopBits": "1", "528 AO-MaxVolts": "5.0000", "XLIGHT Excitation Wheel-State": "1", "470 Shutter-Name": "AnalogIO", "Core-Shutter": "Vincent-D3", "555 AO-Demo": "No", "XLIGHT Dichroic-State": "2", "XYStage-StepSize-Y": "0.1000", "ZPositionUm": 238.455, "XYStage-StepSize-X": "0.1000", "PRIME-FirmwareVersion": "20.28", "PRIME-ClearCycles": "2", "520-Name": "DA Shutter", "405 Shutter-Name": "AnalogIO", "PIEZO-StepSize": "0.0050", "PRIME-ClearMode": "Pre-Exposure", "470 AO-MinVolts": "0.0000", "405 AO-SupportsTriggering": "1", "CameraChannelIndex": 0, "XYStage-StartSpeed": "500.0000", "COM9-StopBits": "2", "405 AO-Description": "NI DAC", "COM4-DelayBetweenCharsMs": "0.0000", "445 AO-Block voltage": "false", "520 AO-TriggerInputLine": "/Dev1/PFI0", "555 AO-Description": "NI DAC", "520 AO-TriggerSampleRate": "100000", "COM4-Verbose": "1", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM4-Name": "COM4", "405 Shutter-Volts": "0.0000", "Vincent-D3-Port": "COM1", "PRIME-PP 4 MIN ADU AFFECTED": "200", "470 Shutter-MinVolts": "0.0000", "PRIME-PP 3 ENABLED": "Yes", "470 AO-Description": "NI DAC", "PRIME-Description": "PVCAM API device adapter", "520 Shutter-MaxVolts": "5.0000", "520 Shutter-Volts": "0.0000", "528 AO-Sequenceable": "1", "PRIME-Binning": "1x1", "555 Shutter-MaxVolts": "5.0000", "528 Shutter-TriggerSequenceLength": "1000", "405 Shutter-Block voltage": "false", "XLIGHT Spin motor-State": "1", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "Vincent-D3-Time to open (ms)": "35.0000", "Vincent-D3-Time to close (ms)": "35.0000", "445 AO-TriggerSampleRate": "100000", "640 Shutter-IOChannel": "Dev1/ao14", "555 AO-Sequenceable": "1", "520 AO-Name": "AnalogIO", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "470 AO-Block voltage": "false", "COM1-Verbose": "1", "COM9-AnswerTimeout": "10000.0000", "528 Shutter-Name": "AnalogIO", "PRIME-Trigger Timeout (secs)": "10", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "NextFrame": 0, "470 AO-TriggerSequenceLength": "1000", "PRIME-TransposeXY": "0", "445 AO-TriggerSequenceLength": "1000", "COM1-Name": "COM1", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "COM9-DataBits": "8", "COM1-AnswerTimeout": "500.0000", "Camera": "", "TE2000-Version": "V2.50-V2.50-V2.10", "528 Shutter-Volts": "0.0000", "XLIGHT Hub-Name": "XLIGHT Hub", "PIEZO-Description": "Ludl stage driver adapter", "PositionName": "Pos34", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSampleRate": "100000", "PRIME-FanSpeedSetpoint": "High", "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "405 AO-TriggerSampleRate": "100000", "470 Shutter-Block voltage": "false", "470-State": "0", "COM3-Parity": "None", "405 AO-Volts": "0.0000", "PRIME-TransposeMirrorY": "0", "PRIME-ChipName": "GS144BSI", "470 AO-Volts": "0.0000", "PRIME-TransposeMirrorX": "0", "445 Shutter-SupportsTriggering": "0", "470 Shutter-SupportsTriggering": "0", "XLIGHT Hub-Description": "XLIGHT hub", "PRIME-PreampOffLimit": "10000", "470 AO-Sequenceable": "1", "528 Shutter-MaxVolts": "5.0000", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "Core-XYStage": "XYStage", "470 AO-TriggerSampleRate": "100000", "PRIME-Timing-PreTriggerDelayNs": "24672000.0000", "528-DA Device": "528 Shutter", "Focus-Description": "Nikon TE2000 focus adapter", "FilterBlock-State": "5", "520 AO-Demo": "No", "COM3-DelayBetweenCharsMs": "0.0000", "470 Shutter-Volts": "0.0000", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "PRIME-PP 2 THRESHOLD": "125", "PRIME-PP 4 THRESHOLD": "75", "XYStage-Acceleration": "4.0000", "470 Shutter-Demo": "No", "PRIME-Name": "PMPCIECam00", "640-DA Device": "640 Shutter", "405 AO-Block voltage": "false", "ElapsedTime-ms": 1420921, "PRIME-Offset": "100", "445 AO-IOChannel": "Dev1/ao1", "640 AO-MaxVolts": "5.0000", "PositionIndex": 34, "PRIME-PP 1 ENABLED": "Yes", "COM1-BaudRate": "9600", "640-State": "0", "COM3-Verbose": "1", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "LudlController-TransmissionDelay": "0", "PRIME-FullWellCapacity": "62000", "PRIME-SMARTStreamingEnabled": "No", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "640 Shutter-Description": "NI DAC", "528-Description": "DA device that is used as a shutter", "COM4-Description": "Serial port driver (boost:asio)", "405 Shutter-Description": "NI DAC", "TE2000-Name": "TE2000", "528 Shutter-Description": "NI DAC", "COM9-Description": "Serial port driver (boost:asio)", "LudlController-Name": "LudlController", "445 Shutter-TriggerSequenceLength": "1000", "PRIME-SerialNumber": "A18B203008", "445 AO-Volts": "0.0000", "PRIME-X-dimension": "1200", "PRIME-ShutterCloseDelay": "0", "XLIGHT Disk slider-State": "2", "Position": "Default", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 AO-IOChannel": "Dev1/ao5", "520 AO-IOChannel": "Dev1/ao4", "528 AO-TriggerSequenceLength": "1000", "405 Shutter-MaxVolts": "5.0000", "470-DA Device": "470 Shutter", "PRIME-FTCapable": "No", "Core-ChannelGroup": "Channels", "555 AO-SupportsTriggering": "0", "PRIME-Y-dimension": "1200", "Frame": 0, "555 Shutter-Sequenceable": "1", "PRIME-Timing-ExposureTimeNs": "499998000.0000", "Core-ImageProcessor": "", "528-State": "0", "520 AO-Block voltage": "false", "XLIGHT Excitation Wheel-Label": "Excitation-2", "640 Shutter-Demo": "No", "COM3-StopBits": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "520 Shutter-IOChannel": "Dev1/ao11", "Width": 1200, "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-SupportsTriggering": "0", "640 AO-TriggerSampleRate": "100000", "520-Description": "DA device that is used as a shutter", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "405 Shutter-Sequenceable": "1", "405 Shutter-SupportsTriggering": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-TriggerSampleRate": "100000", "640 Shutter-MaxVolts": "5.0000", "555 AO-MaxVolts": "5.0000", "520 AO-TriggerSequenceLength": "1000", "COM3-BaudRate": "9600", "COM9-BaudRate": "9600", "Vincent-D3-Channel #": "Ch. #1", "PRIME-PMode": "Normal", "OpticalPath-Label": "Left(100)", "BitDepth": 16, "555 AO-TriggerSequenceLength": "1000", "445 Shutter-Description": "NI DAC", "405 AO-Sequenceable": "1", "PRIME-AcquisitionMethod": "Callbacks", "555 AO-Name": "AnalogIO", "PRIME-ShutterMode": "Pre-Exposure", "COM3-Handshaking": "Off", "445 AO-SupportsTriggering": "0", "ROI": "0-0-1200-1200", "COM9-Verbose": "1", "PRIME-Color": "OFF", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "PRIME-ShutterOpenDelay": "0", "COM1-Parity": "None", "Slice": 7, "SlicePosition": 20, "COM9-DelayBetweenCharsMs": "0.0000", "555-Name": "DA Shutter", "PIEZO-LudlSingleAxisName": "B", "XLIGHT Emission Wheel-Label": "Empty", "640 Shutter-Name": "AnalogIO", "640 AO-Sequenceable": "1", "520 Shutter-Demo": "No", "528 Shutter-Demo": "No", "470-Name": "DA Shutter", "520-DA Device": "520 Shutter", "XYStage-Description": "Ludl XY stage driver adapter", "445 Shutter-MinVolts": "0.0000", "528 AO-TriggerSampleRate": "100000", "405 AO-MinVolts": "0.0000", "405 Shutter-TriggerSequenceLength": "1000", "Height": 1200, "555 AO-Block voltage": "false", "640 Shutter-Volts": "0.0000", "PixelType": "GRAY16", "445 Shutter-Block voltage": "false", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "520 Shutter-TriggerSequenceLength": "1000", "COM9-Name": "COM9", "Core-SLM": "", "555 AO-Volts": "0.0000", "555 Shutter-Block voltage": "false", "XYStage-Speed": "1800.0000", "520 AO-MaxVolts": "5.0000", "528 Shutter-SupportsTriggering": "0", "COM1-Description": "Serial port driver (boost:asio)", "PRIME-MetadataEnabled": "No", "520 Shutter-MinVolts": "0.0000", "470 Shutter-Sequenceable": "1", "PRIME-PreampDelay": "0", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-MinVolts": "0.0000", "ChannelIndex": 4, "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Core-AutoShutter": "1", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "Exposure-ms": 20, "PRIME-PP 2 ENABLED": "Yes", "640 Shutter-TriggerSampleRate": "100000", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "470 Shutter-IOChannel": "Dev1/ao10", "405 AO-Demo": "No", "PixelSizeUm": 1.1, "470 AO-Demo": "No", "470 Shutter-MaxVolts": "5.0000", "Vincent-D3-Name": "Vincent-D3", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FileName": "img_000000000_P-DIC_007.tif", "LudlController-Reset": "Operate", "PRIME-Exposure": "20.00", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "445-Name": "DA Shutter", "555 Shutter-Name": "AnalogIO", "520 Shutter-TriggerSampleRate": "100000", "445 Shutter-IOChannel": "Dev1/ao9", "PRIME-TransposeCorrection": "0", "XYStage-StepSize": "0.1000", "445-Description": "DA device that is used as a shutter", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 AO-IOChannel": "Dev1/ao7", "520 Shutter-Name": "AnalogIO", "COM3-DataBits": "8", "640 AO-Volts": "0.0000", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "555 Shutter-IOChannel": "Dev1/ao13", "WaitInterval": null, "520 Shutter-Sequenceable": "1", "YPositionUm": 545.6, "445-State": "0", "445-DA Device": "445 Shutter", "PRIME-CircularBufferAutoSize": "ON", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "555 AO-TriggerSampleRate": "100000", "445 Shutter-Volts": "0.0000", "XLIGHT Hub-Port": "COM3", "Core-AutoFocus": "", "COM4-Handshaking": "Off", "528 Shutter-MinVolts": "0.0000", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "445 Shutter-Name": "AnalogIO", "528 Shutter-Sequenceable": "1", "XYStage-TransposeMirrorX": "0", "XYStage-TransposeMirrorY": "0", "PRIME-ReadoutRate": "100MHz 16bit", "XYStage-Name": "Stage", "640 AO-SupportsTriggering": "0", "640-Name": "DA Shutter", "640 Shutter-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "640 AO-Block voltage": "false", "445 Shutter-Sequenceable": "1", "470 AO-MaxVolts": "5.0000", "COM4-BaudRate": "9600", "470-Description": "DA device that is used as a shutter", "520 Shutter-Description": "NI DAC", "PRIME-CCDTemperature": "-14.8500", "Time": "2019-12-19 09:59:51 -0500", "640 AO-TriggerSequenceLength": "1000", "470 AO-Name": "AnalogIO", "XLIGHT Disk slider-Label": "Disk Pos 40um", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "Binning": "1x1", "TE2000-ModelType": "1", "COM3-AnswerTimeout": "500.0000", "555 Shutter-SupportsTriggering": "0", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "TE2000-Port": "COM4", "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Description": "NI DAC", "COM4-AnswerTimeout": "500.0000", "COM1-DelayBetweenCharsMs": "0.0000", "FilterBlock-Label": "Confocal", "445 AO-Name": "AnalogIO", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-Timing-ReadoutTimeNs": "24672000.0000", "528 AO-Demo": "No", "520 AO-Sequenceable": "1", "555-Description": "DA device that is used as a shutter", "405-DA Device": "405 Shutter", "405 AO-TriggerSequenceLength": "1000", "640 AO-MinVolts": "0.0000", "640 Shutter-Block voltage": "false", "FrameIndex": 0, "PRIME-Port": "CMOS", "555 AO-MinVolts": "0.0000", "COM3-Description": "Serial port driver (boost:asio)", "528 AO-Description": "NI DAC", "555 AO-IOChannel": "Dev1/ao6", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "555 AO-TriggerInputLine": "/Dev1/PFI0", "PRIME-ActualInterval-ms": "390.5200", "LudlController-Port": "COM9", "445 Shutter-Demo": "No", "PRIME-Trigger-Expose Out-Mux": "1", "SliceIndex": 7, "PRIME-CCDTemperatureSetPoint": "-15.0000", "405 Shutter-MinVolts": "0.0000", "PRIME-ExposeOutMode": "First Row", "AxisPositions": null, "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Volts": "0.0000", "528-Name": "DA Shutter", "555-State": "0", "XLIGHT Dichroic-Label": "Di03-R635-t1", "445 AO-Sequenceable": "1", "405-Name": "DA Shutter", "405 AO-IOChannel": "Dev1/ao0", "Core-Camera": "PRIME", "Focus-Name": "Focus", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "405 Shutter-IOChannel": "Dev1/ao8", "640 AO-Demo": "No", "640 Shutter-SupportsTriggering": "0", "445 AO-Demo": "No", "555-DA Device": "555 Shutter", "470 Shutter-Description": "NI DAC", "528 AO-Volts": "0.0000", "COM1-DataBits": "8", "Vincent-D3-Address": "x", "520 AO-SupportsTriggering": "0", "405-Description": "DA device that is used as a shutter", "640 Shutter-MinVolts": "0.0000", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "405 AO-Name": "AnalogIO", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "640 AO-TriggerInputLine": "/Dev1/PFI0", "405 AO-TriggerInputLine": "/Dev1/PFI0", "640-Description": "DA device that is used as a shutter", "445 Shutter-TriggerSampleRate": "100000", "470 Shutter-TriggerSequenceLength": "1000", "COM1-StopBits": "1", "520 Shutter-SupportsTriggering": "0", "405-State": "0", "Core-Initialize": "1", "XLIGHT Emission Wheel-State": "3", "520 AO-Description": "NI DAC", "528 AO-TriggerInputLine": "/Dev1/PFI0", "445 Shutter-MaxVolts": "5.0000", "COM9-Handshaking": "Off", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "XLIGHT Spin motor-Label": "On (spinning)", "PRIME-PP 5 ENABLED": "No", "UUID": "db77cff4-559c-4bb6-b539-cdf68f73a2fb", "PRIME-TriggerMode": "Internal Trigger", "COM1-Handshaking": "Off", "PRIME-Timing-PostTriggerDelayNs": "49344000.0000", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "Focus-Position": "17741.5000", "528 Shutter-Block voltage": "false", "Channel": "P-DIC", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "555 Shutter-Demo": "No", "405 Shutter-Demo": "No", "528 AO-Name": "AnalogIO", "520-State": "0", "Core-Galvo": "", "405 Shutter-TriggerSampleRate": "100000", "Focus-FocusStepSize_(nm)": "100", "PRIME-PixelType": "16bit", "COM4-DataBits": "8", "470 AO-IOChannel": "Dev1/ao2", "520 AO-MinVolts": "0.0000", "COM9-Parity": "None", "PRIME-CircularBufferEnabled": "ON", "PRIME-PP 6 Reset": "No", "640 AO-Description": "NI DAC", "Source": "PRIME", "445 AO-MinVolts": "0.0000", "PIEZO-Name": "Stage", "445 AO-Description": "NI DAC", "PRIME-CircularBufferFrameCount": "46", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "520 AO-Volts": "0.0000", "528 Shutter-TriggerSampleRate": "100000", "XPositionUm": -1324.9, "OpticalPath-State": "4", "PRIME-PP 3 THRESHOLD": "80", "PRIME-PVCAM Version": "3.7.1", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Gain": "1-HDR", "640 Shutter-TriggerSequenceLength": "1000", "528 AO-SupportsTriggering": "0", "445 AO-TriggerInputLine": "/Dev1/PFI0", "OpticalPath-Name": "OpticalPath", "PRIME-PP 4 ENABLED": "Yes", "PRIME-PVCAM Adapter Version": "1.3.29", "COM3-Name": "COM3", "405 AO-MaxVolts": "5.0000", "520 Shutter-Block voltage": "false", "PRIME-Timing-ClearingTimeNs": "49344000.0000", "Core-Focus": "PIEZO", "528 AO-Block voltage": "false", "TE2000-Description": "Nikon TE2000 hub", "528 AO-MinVolts": "0.0000", "COM4-Parity": "None", "Summary": { "Slices": 11, "ChContrastMin": [ 0, 0, 0, 0, 0, 0 ], "PixelAspect": 1, "SlicesFirst": true, "Interval_ms": 0, "Time": "2019-12-19 09:59:21 -0500", "Prefix": "seq_13", "InitialPositionList": [ { "Label": "Pos0", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.21], "Focus": [17741.5], "XYStage": [ 44542.200000000004, 31636.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos1", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.22], "Focus": [17741.4], "XYStage": [ 43801.100000000006, 31637.9 ] }, "GridColumnIndex": 0 }, { "Label": "Pos2", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.5], "XYStage": [ 38561.3, 32040.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos3", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.185], "Focus": [17741.3], "XYStage": [ 37698.4, 32041.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos4", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.16], "Focus": [17741.5], "XYStage": [ 32375.4, 31758.9 ] }, "GridColumnIndex": 0 }, { "Label": "Pos5", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.17000000000002], "Focus": [17741.3], "XYStage": [ 31770.7, 31758.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos6", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.4], "XYStage": [ 26536.800000000003, 31068.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos7", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.205], "Focus": [17741.4], "XYStage": [ 25420.2, 31068.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos8", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.85], "Focus": [17741.3], "XYStage": [ 21059, 31508 ] }, "GridColumnIndex": 0 }, { "Label": "Pos9", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.82], "Focus": [17741.3], "XYStage": [ 19731.9, 31506.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos10", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.79], "Focus": [17741.4], "XYStage": [ 14528.5, 32007.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos11", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.83], "Focus": [17741.2], "XYStage": [ 13830.300000000001, 32009.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos12", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.79], "Focus": [17741.3], "XYStage": [ 8775.300000000001, 32017.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos13", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.815], "Focus": [17741.3], "XYStage": [ 8109.200000000001, 32008.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos14", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.805], "Focus": [17741.3], "XYStage": [ 3483.3, 31908.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos15", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.82], "Focus": [17741.2], "XYStage": [ 2686.8, 31903.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos16", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [211.48000000000002], "Focus": [17741.1], "XYStage": [ -1978.9, 31973.600000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos17", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [211.51500000000001], "Focus": [17741.1], "XYStage": [ -2957.8, 31982.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos18", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.195], "Focus": [17741.5], "XYStage": [ 47747.4, -576.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos19", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.23000000000002], "Focus": [17741.6], "XYStage": [ 47053.9, -576.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos20", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.205], "Focus": [17741.4], "XYStage": [ 41871.200000000004, -479.8 ] }, "GridColumnIndex": 0 }, { "Label": "Pos21", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.4], "XYStage": [ 40882.600000000006, -392 ] }, "GridColumnIndex": 0 }, { "Label": "Pos22", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.24], "Focus": [17741.4], "XYStage": [ 35905.6, -135.3 ] }, "GridColumnIndex": 0 }, { "Label": "Pos23", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.195], "Focus": [17741.5], "XYStage": [ 34990.9, -132.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos24", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.46], "Focus": [17741.4], "XYStage": [ 29678.100000000002, -958.1 ] }, "GridColumnIndex": 0 }, { "Label": "Pos25", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.44], "Focus": [17741.5], "XYStage": [ 28983.600000000002, -939.4000000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos26", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.475], "Focus": [17741.5], "XYStage": [ 23019.7, -122 ] }, "GridColumnIndex": 0 }, { "Label": "Pos27", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.44], "Focus": [17741.7], "XYStage": [ 22428, -116.80000000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos28", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.46], "Focus": [17741.7], "XYStage": [ 16364.900000000001, -181.10000000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos29", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.48000000000002], "Focus": [17741.5], "XYStage": [ 15712.1, -180 ] }, "GridColumnIndex": 0 }, { "Label": "Pos30", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.45000000000002], "Focus": [17741.7], "XYStage": [ 10346.1, 77.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos31", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.49], "Focus": [17741.4], "XYStage": [ 9755.5, 85.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos32", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.475], "Focus": [17741.5], "XYStage": [ 4775.5, 293.90000000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos33", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.445], "Focus": [17741.4], "XYStage": [ 3954.9, 293.90000000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos34", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.455], "Focus": [17741.4], "XYStage": [ -1324.9, 545.6 ] }, "GridColumnIndex": 0 }, { "Label": "Pos35", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.445], "Focus": [17741.3], "XYStage": [ -2097, 576.6 ] }, "GridColumnIndex": 0 } ], "z-step_um": 10, "GridRow": 0, "BitDepth": 16, "Depth": 2, "Channels": 6, "Source": "Micro-Manager", "ChColors": [ -16711681, -256, -65281, -1, -1, -16776961 ], "MetadataVersion": 10, "KeepShutterOpenChannels": true, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536, 65536 ], "CustomIntervals_ms": [], "Height": 1200, "MicroManagerVersion": "1.4.23 20180219", "GridColumn": 0, "UUID": "18808e7c-f2e6-47b3-90d8-1d779ce87dbd", "PixelType": "GRAY16", "Width": 1200, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\Yan\\20191218-YC113-S7S8-miseq", "Frames": 1, "Comment": "", "UserName": "Nikon", "PixelSize_um": 1.1, "ROI": [ 0, 0, 1200, 1200 ], "Date": "2019-12-19", "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC", "P-DAPI-high" ], "KeepShutterOpenSlices": true, "IJType": 1, "ComputerName": "ZadorConfocal2", "Positions": 36, "PositionIndex": 34, "CameraTimeout": 20000, "TimeFirst": false }, "640 AO-Name": "AnalogIO", "Vincent-D3-Command": "Close" }, "FrameKey-0-4-8": { "COM4-StopBits": "1", "528 AO-MaxVolts": "5.0000", "XLIGHT Excitation Wheel-State": "1", "470 Shutter-Name": "AnalogIO", "Core-Shutter": "Vincent-D3", "555 AO-Demo": "No", "XLIGHT Dichroic-State": "2", "XYStage-StepSize-Y": "0.1000", "ZPositionUm": 248.455, "XYStage-StepSize-X": "0.1000", "PRIME-FirmwareVersion": "20.28", "PRIME-ClearCycles": "2", "520-Name": "DA Shutter", "405 Shutter-Name": "AnalogIO", "PIEZO-StepSize": "0.0050", "PRIME-ClearMode": "Pre-Exposure", "470 AO-MinVolts": "0.0000", "405 AO-SupportsTriggering": "1", "CameraChannelIndex": 0, "XYStage-StartSpeed": "500.0000", "COM9-StopBits": "2", "405 AO-Description": "NI DAC", "COM4-DelayBetweenCharsMs": "0.0000", "445 AO-Block voltage": "false", "520 AO-TriggerInputLine": "/Dev1/PFI0", "555 AO-Description": "NI DAC", "520 AO-TriggerSampleRate": "100000", "COM4-Verbose": "1", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM4-Name": "COM4", "405 Shutter-Volts": "0.0000", "Vincent-D3-Port": "COM1", "PRIME-PP 4 MIN ADU AFFECTED": "200", "470 Shutter-MinVolts": "0.0000", "PRIME-PP 3 ENABLED": "Yes", "470 AO-Description": "NI DAC", "PRIME-Description": "PVCAM API device adapter", "520 Shutter-MaxVolts": "5.0000", "520 Shutter-Volts": "0.0000", "528 AO-Sequenceable": "1", "PRIME-Binning": "1x1", "555 Shutter-MaxVolts": "5.0000", "528 Shutter-TriggerSequenceLength": "1000", "405 Shutter-Block voltage": "false", "XLIGHT Spin motor-State": "1", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "Vincent-D3-Time to open (ms)": "35.0000", "Vincent-D3-Time to close (ms)": "35.0000", "445 AO-TriggerSampleRate": "100000", "640 Shutter-IOChannel": "Dev1/ao14", "555 AO-Sequenceable": "1", "520 AO-Name": "AnalogIO", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "470 AO-Block voltage": "false", "COM1-Verbose": "1", "COM9-AnswerTimeout": "10000.0000", "528 Shutter-Name": "AnalogIO", "PRIME-Trigger Timeout (secs)": "10", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "NextFrame": 0, "470 AO-TriggerSequenceLength": "1000", "PRIME-TransposeXY": "0", "445 AO-TriggerSequenceLength": "1000", "COM1-Name": "COM1", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "COM9-DataBits": "8", "COM1-AnswerTimeout": "500.0000", "Camera": "", "TE2000-Version": "V2.50-V2.50-V2.10", "528 Shutter-Volts": "0.0000", "XLIGHT Hub-Name": "XLIGHT Hub", "PIEZO-Description": "Ludl stage driver adapter", "PositionName": "Pos34", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSampleRate": "100000", "PRIME-FanSpeedSetpoint": "High", "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "405 AO-TriggerSampleRate": "100000", "470 Shutter-Block voltage": "false", "470-State": "0", "COM3-Parity": "None", "405 AO-Volts": "0.0000", "PRIME-TransposeMirrorY": "0", "PRIME-ChipName": "GS144BSI", "470 AO-Volts": "0.0000", "PRIME-TransposeMirrorX": "0", "445 Shutter-SupportsTriggering": "0", "470 Shutter-SupportsTriggering": "0", "XLIGHT Hub-Description": "XLIGHT hub", "PRIME-PreampOffLimit": "10000", "470 AO-Sequenceable": "1", "528 Shutter-MaxVolts": "5.0000", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "Core-XYStage": "XYStage", "470 AO-TriggerSampleRate": "100000", "PRIME-Timing-PreTriggerDelayNs": "24672000.0000", "528-DA Device": "528 Shutter", "Focus-Description": "Nikon TE2000 focus adapter", "FilterBlock-State": "5", "520 AO-Demo": "No", "COM3-DelayBetweenCharsMs": "0.0000", "470 Shutter-Volts": "0.0000", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "PRIME-PP 2 THRESHOLD": "125", "PRIME-PP 4 THRESHOLD": "75", "XYStage-Acceleration": "4.0000", "470 Shutter-Demo": "No", "PRIME-Name": "PMPCIECam00", "640-DA Device": "640 Shutter", "405 AO-Block voltage": "false", "ElapsedTime-ms": 1421175, "PRIME-Offset": "100", "445 AO-IOChannel": "Dev1/ao1", "640 AO-MaxVolts": "5.0000", "PositionIndex": 34, "PRIME-PP 1 ENABLED": "Yes", "COM1-BaudRate": "9600", "640-State": "0", "COM3-Verbose": "1", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "LudlController-TransmissionDelay": "0", "PRIME-FullWellCapacity": "62000", "PRIME-SMARTStreamingEnabled": "No", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "640 Shutter-Description": "NI DAC", "528-Description": "DA device that is used as a shutter", "COM4-Description": "Serial port driver (boost:asio)", "405 Shutter-Description": "NI DAC", "TE2000-Name": "TE2000", "528 Shutter-Description": "NI DAC", "COM9-Description": "Serial port driver (boost:asio)", "LudlController-Name": "LudlController", "445 Shutter-TriggerSequenceLength": "1000", "PRIME-SerialNumber": "A18B203008", "445 AO-Volts": "0.0000", "PRIME-X-dimension": "1200", "PRIME-ShutterCloseDelay": "0", "XLIGHT Disk slider-State": "2", "Position": "Default", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 AO-IOChannel": "Dev1/ao5", "520 AO-IOChannel": "Dev1/ao4", "528 AO-TriggerSequenceLength": "1000", "405 Shutter-MaxVolts": "5.0000", "470-DA Device": "470 Shutter", "PRIME-FTCapable": "No", "Core-ChannelGroup": "Channels", "555 AO-SupportsTriggering": "0", "PRIME-Y-dimension": "1200", "Frame": 0, "555 Shutter-Sequenceable": "1", "PRIME-Timing-ExposureTimeNs": "499998000.0000", "Core-ImageProcessor": "", "528-State": "0", "520 AO-Block voltage": "false", "XLIGHT Excitation Wheel-Label": "Excitation-2", "640 Shutter-Demo": "No", "COM3-StopBits": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "520 Shutter-IOChannel": "Dev1/ao11", "Width": 1200, "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-SupportsTriggering": "0", "640 AO-TriggerSampleRate": "100000", "520-Description": "DA device that is used as a shutter", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "405 Shutter-Sequenceable": "1", "405 Shutter-SupportsTriggering": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-TriggerSampleRate": "100000", "640 Shutter-MaxVolts": "5.0000", "555 AO-MaxVolts": "5.0000", "520 AO-TriggerSequenceLength": "1000", "COM3-BaudRate": "9600", "COM9-BaudRate": "9600", "Vincent-D3-Channel #": "Ch. #1", "PRIME-PMode": "Normal", "OpticalPath-Label": "Left(100)", "BitDepth": 16, "555 AO-TriggerSequenceLength": "1000", "445 Shutter-Description": "NI DAC", "405 AO-Sequenceable": "1", "PRIME-AcquisitionMethod": "Callbacks", "555 AO-Name": "AnalogIO", "PRIME-ShutterMode": "Pre-Exposure", "COM3-Handshaking": "Off", "445 AO-SupportsTriggering": "0", "ROI": "0-0-1200-1200", "COM9-Verbose": "1", "PRIME-Color": "OFF", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "PRIME-ShutterOpenDelay": "0", "COM1-Parity": "None", "Slice": 8, "SlicePosition": 30, "COM9-DelayBetweenCharsMs": "0.0000", "555-Name": "DA Shutter", "PIEZO-LudlSingleAxisName": "B", "XLIGHT Emission Wheel-Label": "Empty", "640 Shutter-Name": "AnalogIO", "640 AO-Sequenceable": "1", "520 Shutter-Demo": "No", "528 Shutter-Demo": "No", "470-Name": "DA Shutter", "520-DA Device": "520 Shutter", "XYStage-Description": "Ludl XY stage driver adapter", "445 Shutter-MinVolts": "0.0000", "528 AO-TriggerSampleRate": "100000", "405 AO-MinVolts": "0.0000", "405 Shutter-TriggerSequenceLength": "1000", "Height": 1200, "555 AO-Block voltage": "false", "640 Shutter-Volts": "0.0000", "PixelType": "GRAY16", "445 Shutter-Block voltage": "false", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "520 Shutter-TriggerSequenceLength": "1000", "COM9-Name": "COM9", "Core-SLM": "", "555 AO-Volts": "0.0000", "555 Shutter-Block voltage": "false", "XYStage-Speed": "1800.0000", "520 AO-MaxVolts": "5.0000", "528 Shutter-SupportsTriggering": "0", "COM1-Description": "Serial port driver (boost:asio)", "PRIME-MetadataEnabled": "No", "520 Shutter-MinVolts": "0.0000", "470 Shutter-Sequenceable": "1", "PRIME-PreampDelay": "0", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-MinVolts": "0.0000", "ChannelIndex": 4, "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Core-AutoShutter": "1", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "Exposure-ms": 20, "PRIME-PP 2 ENABLED": "Yes", "640 Shutter-TriggerSampleRate": "100000", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "470 Shutter-IOChannel": "Dev1/ao10", "405 AO-Demo": "No", "PixelSizeUm": 1.1, "470 AO-Demo": "No", "470 Shutter-MaxVolts": "5.0000", "Vincent-D3-Name": "Vincent-D3", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FileName": "img_000000000_P-DIC_008.tif", "LudlController-Reset": "Operate", "PRIME-Exposure": "20.00", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "445-Name": "DA Shutter", "555 Shutter-Name": "AnalogIO", "520 Shutter-TriggerSampleRate": "100000", "445 Shutter-IOChannel": "Dev1/ao9", "PRIME-TransposeCorrection": "0", "XYStage-StepSize": "0.1000", "445-Description": "DA device that is used as a shutter", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 AO-IOChannel": "Dev1/ao7", "520 Shutter-Name": "AnalogIO", "COM3-DataBits": "8", "640 AO-Volts": "0.0000", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "555 Shutter-IOChannel": "Dev1/ao13", "WaitInterval": null, "520 Shutter-Sequenceable": "1", "YPositionUm": 545.6, "445-State": "0", "445-DA Device": "445 Shutter", "PRIME-CircularBufferAutoSize": "ON", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "555 AO-TriggerSampleRate": "100000", "445 Shutter-Volts": "0.0000", "XLIGHT Hub-Port": "COM3", "Core-AutoFocus": "", "COM4-Handshaking": "Off", "528 Shutter-MinVolts": "0.0000", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "445 Shutter-Name": "AnalogIO", "528 Shutter-Sequenceable": "1", "XYStage-TransposeMirrorX": "0", "XYStage-TransposeMirrorY": "0", "PRIME-ReadoutRate": "100MHz 16bit", "XYStage-Name": "Stage", "640 AO-SupportsTriggering": "0", "640-Name": "DA Shutter", "640 Shutter-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "640 AO-Block voltage": "false", "445 Shutter-Sequenceable": "1", "470 AO-MaxVolts": "5.0000", "COM4-BaudRate": "9600", "470-Description": "DA device that is used as a shutter", "520 Shutter-Description": "NI DAC", "PRIME-CCDTemperature": "-14.8500", "Time": "2019-12-19 09:59:51 -0500", "640 AO-TriggerSequenceLength": "1000", "470 AO-Name": "AnalogIO", "XLIGHT Disk slider-Label": "Disk Pos 40um", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "Binning": "1x1", "TE2000-ModelType": "1", "COM3-AnswerTimeout": "500.0000", "555 Shutter-SupportsTriggering": "0", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "TE2000-Port": "COM4", "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Description": "NI DAC", "COM4-AnswerTimeout": "500.0000", "COM1-DelayBetweenCharsMs": "0.0000", "FilterBlock-Label": "Confocal", "445 AO-Name": "AnalogIO", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-Timing-ReadoutTimeNs": "24672000.0000", "528 AO-Demo": "No", "520 AO-Sequenceable": "1", "555-Description": "DA device that is used as a shutter", "405-DA Device": "405 Shutter", "405 AO-TriggerSequenceLength": "1000", "640 AO-MinVolts": "0.0000", "640 Shutter-Block voltage": "false", "FrameIndex": 0, "PRIME-Port": "CMOS", "555 AO-MinVolts": "0.0000", "COM3-Description": "Serial port driver (boost:asio)", "528 AO-Description": "NI DAC", "555 AO-IOChannel": "Dev1/ao6", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "555 AO-TriggerInputLine": "/Dev1/PFI0", "PRIME-ActualInterval-ms": "390.5200", "LudlController-Port": "COM9", "445 Shutter-Demo": "No", "PRIME-Trigger-Expose Out-Mux": "1", "SliceIndex": 8, "PRIME-CCDTemperatureSetPoint": "-15.0000", "405 Shutter-MinVolts": "0.0000", "PRIME-ExposeOutMode": "First Row", "AxisPositions": null, "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Volts": "0.0000", "528-Name": "DA Shutter", "555-State": "0", "XLIGHT Dichroic-Label": "Di03-R635-t1", "445 AO-Sequenceable": "1", "405-Name": "DA Shutter", "405 AO-IOChannel": "Dev1/ao0", "Core-Camera": "PRIME", "Focus-Name": "Focus", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "405 Shutter-IOChannel": "Dev1/ao8", "640 AO-Demo": "No", "640 Shutter-SupportsTriggering": "0", "445 AO-Demo": "No", "555-DA Device": "555 Shutter", "470 Shutter-Description": "NI DAC", "528 AO-Volts": "0.0000", "COM1-DataBits": "8", "Vincent-D3-Address": "x", "520 AO-SupportsTriggering": "0", "405-Description": "DA device that is used as a shutter", "640 Shutter-MinVolts": "0.0000", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "405 AO-Name": "AnalogIO", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "640 AO-TriggerInputLine": "/Dev1/PFI0", "405 AO-TriggerInputLine": "/Dev1/PFI0", "640-Description": "DA device that is used as a shutter", "445 Shutter-TriggerSampleRate": "100000", "470 Shutter-TriggerSequenceLength": "1000", "COM1-StopBits": "1", "520 Shutter-SupportsTriggering": "0", "405-State": "0", "Core-Initialize": "1", "XLIGHT Emission Wheel-State": "3", "520 AO-Description": "NI DAC", "528 AO-TriggerInputLine": "/Dev1/PFI0", "445 Shutter-MaxVolts": "5.0000", "COM9-Handshaking": "Off", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "XLIGHT Spin motor-Label": "On (spinning)", "PRIME-PP 5 ENABLED": "No", "UUID": "e60097cc-a797-4df5-9e57-ea40bf30a656", "PRIME-TriggerMode": "Internal Trigger", "COM1-Handshaking": "Off", "PRIME-Timing-PostTriggerDelayNs": "49344000.0000", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "Focus-Position": "17741.5000", "528 Shutter-Block voltage": "false", "Channel": "P-DIC", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "555 Shutter-Demo": "No", "405 Shutter-Demo": "No", "528 AO-Name": "AnalogIO", "520-State": "0", "Core-Galvo": "", "405 Shutter-TriggerSampleRate": "100000", "Focus-FocusStepSize_(nm)": "100", "PRIME-PixelType": "16bit", "COM4-DataBits": "8", "470 AO-IOChannel": "Dev1/ao2", "520 AO-MinVolts": "0.0000", "COM9-Parity": "None", "PRIME-CircularBufferEnabled": "ON", "PRIME-PP 6 Reset": "No", "640 AO-Description": "NI DAC", "Source": "PRIME", "445 AO-MinVolts": "0.0000", "PIEZO-Name": "Stage", "445 AO-Description": "NI DAC", "PRIME-CircularBufferFrameCount": "46", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "520 AO-Volts": "0.0000", "528 Shutter-TriggerSampleRate": "100000", "XPositionUm": -1324.9, "OpticalPath-State": "4", "PRIME-PP 3 THRESHOLD": "80", "PRIME-PVCAM Version": "3.7.1", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Gain": "1-HDR", "640 Shutter-TriggerSequenceLength": "1000", "528 AO-SupportsTriggering": "0", "445 AO-TriggerInputLine": "/Dev1/PFI0", "OpticalPath-Name": "OpticalPath", "PRIME-PP 4 ENABLED": "Yes", "PRIME-PVCAM Adapter Version": "1.3.29", "COM3-Name": "COM3", "405 AO-MaxVolts": "5.0000", "520 Shutter-Block voltage": "false", "PRIME-Timing-ClearingTimeNs": "49344000.0000", "Core-Focus": "PIEZO", "528 AO-Block voltage": "false", "TE2000-Description": "Nikon TE2000 hub", "528 AO-MinVolts": "0.0000", "COM4-Parity": "None", "Summary": { "Slices": 11, "ChContrastMin": [ 0, 0, 0, 0, 0, 0 ], "PixelAspect": 1, "SlicesFirst": true, "Interval_ms": 0, "Time": "2019-12-19 09:59:21 -0500", "Prefix": "seq_13", "InitialPositionList": [ { "Label": "Pos0", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.21], "Focus": [17741.5], "XYStage": [ 44542.200000000004, 31636.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos1", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.22], "Focus": [17741.4], "XYStage": [ 43801.100000000006, 31637.9 ] }, "GridColumnIndex": 0 }, { "Label": "Pos2", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.5], "XYStage": [ 38561.3, 32040.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos3", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.185], "Focus": [17741.3], "XYStage": [ 37698.4, 32041.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos4", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.16], "Focus": [17741.5], "XYStage": [ 32375.4, 31758.9 ] }, "GridColumnIndex": 0 }, { "Label": "Pos5", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.17000000000002], "Focus": [17741.3], "XYStage": [ 31770.7, 31758.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos6", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.4], "XYStage": [ 26536.800000000003, 31068.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos7", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.205], "Focus": [17741.4], "XYStage": [ 25420.2, 31068.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos8", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.85], "Focus": [17741.3], "XYStage": [ 21059, 31508 ] }, "GridColumnIndex": 0 }, { "Label": "Pos9", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.82], "Focus": [17741.3], "XYStage": [ 19731.9, 31506.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos10", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.79], "Focus": [17741.4], "XYStage": [ 14528.5, 32007.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos11", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.83], "Focus": [17741.2], "XYStage": [ 13830.300000000001, 32009.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos12", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.79], "Focus": [17741.3], "XYStage": [ 8775.300000000001, 32017.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos13", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.815], "Focus": [17741.3], "XYStage": [ 8109.200000000001, 32008.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos14", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.805], "Focus": [17741.3], "XYStage": [ 3483.3, 31908.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos15", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.82], "Focus": [17741.2], "XYStage": [ 2686.8, 31903.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos16", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [211.48000000000002], "Focus": [17741.1], "XYStage": [ -1978.9, 31973.600000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos17", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [211.51500000000001], "Focus": [17741.1], "XYStage": [ -2957.8, 31982.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos18", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.195], "Focus": [17741.5], "XYStage": [ 47747.4, -576.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos19", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.23000000000002], "Focus": [17741.6], "XYStage": [ 47053.9, -576.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos20", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.205], "Focus": [17741.4], "XYStage": [ 41871.200000000004, -479.8 ] }, "GridColumnIndex": 0 }, { "Label": "Pos21", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.4], "XYStage": [ 40882.600000000006, -392 ] }, "GridColumnIndex": 0 }, { "Label": "Pos22", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.24], "Focus": [17741.4], "XYStage": [ 35905.6, -135.3 ] }, "GridColumnIndex": 0 }, { "Label": "Pos23", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.195], "Focus": [17741.5], "XYStage": [ 34990.9, -132.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos24", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.46], "Focus": [17741.4], "XYStage": [ 29678.100000000002, -958.1 ] }, "GridColumnIndex": 0 }, { "Label": "Pos25", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.44], "Focus": [17741.5], "XYStage": [ 28983.600000000002, -939.4000000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos26", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.475], "Focus": [17741.5], "XYStage": [ 23019.7, -122 ] }, "GridColumnIndex": 0 }, { "Label": "Pos27", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.44], "Focus": [17741.7], "XYStage": [ 22428, -116.80000000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos28", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.46], "Focus": [17741.7], "XYStage": [ 16364.900000000001, -181.10000000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos29", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.48000000000002], "Focus": [17741.5], "XYStage": [ 15712.1, -180 ] }, "GridColumnIndex": 0 }, { "Label": "Pos30", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.45000000000002], "Focus": [17741.7], "XYStage": [ 10346.1, 77.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos31", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.49], "Focus": [17741.4], "XYStage": [ 9755.5, 85.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos32", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.475], "Focus": [17741.5], "XYStage": [ 4775.5, 293.90000000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos33", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.445], "Focus": [17741.4], "XYStage": [ 3954.9, 293.90000000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos34", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.455], "Focus": [17741.4], "XYStage": [ -1324.9, 545.6 ] }, "GridColumnIndex": 0 }, { "Label": "Pos35", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.445], "Focus": [17741.3], "XYStage": [ -2097, 576.6 ] }, "GridColumnIndex": 0 } ], "z-step_um": 10, "GridRow": 0, "BitDepth": 16, "Depth": 2, "Channels": 6, "Source": "Micro-Manager", "ChColors": [ -16711681, -256, -65281, -1, -1, -16776961 ], "MetadataVersion": 10, "KeepShutterOpenChannels": true, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536, 65536 ], "CustomIntervals_ms": [], "Height": 1200, "MicroManagerVersion": "1.4.23 20180219", "GridColumn": 0, "UUID": "18808e7c-f2e6-47b3-90d8-1d779ce87dbd", "PixelType": "GRAY16", "Width": 1200, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\Yan\\20191218-YC113-S7S8-miseq", "Frames": 1, "Comment": "", "UserName": "Nikon", "PixelSize_um": 1.1, "ROI": [ 0, 0, 1200, 1200 ], "Date": "2019-12-19", "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC", "P-DAPI-high" ], "KeepShutterOpenSlices": true, "IJType": 1, "ComputerName": "ZadorConfocal2", "Positions": 36, "PositionIndex": 34, "CameraTimeout": 20000, "TimeFirst": false }, "640 AO-Name": "AnalogIO", "Vincent-D3-Command": "Close" }, "FrameKey-0-4-9": { "COM4-StopBits": "1", "528 AO-MaxVolts": "5.0000", "XLIGHT Excitation Wheel-State": "1", "470 Shutter-Name": "AnalogIO", "Core-Shutter": "Vincent-D3", "555 AO-Demo": "No", "XLIGHT Dichroic-State": "2", "XYStage-StepSize-Y": "0.1000", "ZPositionUm": 258.45500000000004, "XYStage-StepSize-X": "0.1000", "PRIME-FirmwareVersion": "20.28", "PRIME-ClearCycles": "2", "520-Name": "DA Shutter", "405 Shutter-Name": "AnalogIO", "PIEZO-StepSize": "0.0050", "PRIME-ClearMode": "Pre-Exposure", "470 AO-MinVolts": "0.0000", "405 AO-SupportsTriggering": "1", "CameraChannelIndex": 0, "XYStage-StartSpeed": "500.0000", "COM9-StopBits": "2", "405 AO-Description": "NI DAC", "COM4-DelayBetweenCharsMs": "0.0000", "445 AO-Block voltage": "false", "520 AO-TriggerInputLine": "/Dev1/PFI0", "555 AO-Description": "NI DAC", "520 AO-TriggerSampleRate": "100000", "COM4-Verbose": "1", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM4-Name": "COM4", "405 Shutter-Volts": "0.0000", "Vincent-D3-Port": "COM1", "PRIME-PP 4 MIN ADU AFFECTED": "200", "470 Shutter-MinVolts": "0.0000", "PRIME-PP 3 ENABLED": "Yes", "470 AO-Description": "NI DAC", "PRIME-Description": "PVCAM API device adapter", "520 Shutter-MaxVolts": "5.0000", "520 Shutter-Volts": "0.0000", "528 AO-Sequenceable": "1", "PRIME-Binning": "1x1", "555 Shutter-MaxVolts": "5.0000", "528 Shutter-TriggerSequenceLength": "1000", "405 Shutter-Block voltage": "false", "XLIGHT Spin motor-State": "1", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "Vincent-D3-Time to open (ms)": "35.0000", "Vincent-D3-Time to close (ms)": "35.0000", "445 AO-TriggerSampleRate": "100000", "640 Shutter-IOChannel": "Dev1/ao14", "555 AO-Sequenceable": "1", "520 AO-Name": "AnalogIO", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "470 AO-Block voltage": "false", "COM1-Verbose": "1", "COM9-AnswerTimeout": "10000.0000", "528 Shutter-Name": "AnalogIO", "PRIME-Trigger Timeout (secs)": "10", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "NextFrame": 0, "470 AO-TriggerSequenceLength": "1000", "PRIME-TransposeXY": "0", "445 AO-TriggerSequenceLength": "1000", "COM1-Name": "COM1", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "COM9-DataBits": "8", "COM1-AnswerTimeout": "500.0000", "Camera": "", "TE2000-Version": "V2.50-V2.50-V2.10", "528 Shutter-Volts": "0.0000", "XLIGHT Hub-Name": "XLIGHT Hub", "PIEZO-Description": "Ludl stage driver adapter", "PositionName": "Pos34", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSampleRate": "100000", "PRIME-FanSpeedSetpoint": "High", "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "405 AO-TriggerSampleRate": "100000", "470 Shutter-Block voltage": "false", "470-State": "0", "COM3-Parity": "None", "405 AO-Volts": "0.0000", "PRIME-TransposeMirrorY": "0", "PRIME-ChipName": "GS144BSI", "470 AO-Volts": "0.0000", "PRIME-TransposeMirrorX": "0", "445 Shutter-SupportsTriggering": "0", "470 Shutter-SupportsTriggering": "0", "XLIGHT Hub-Description": "XLIGHT hub", "PRIME-PreampOffLimit": "10000", "470 AO-Sequenceable": "1", "528 Shutter-MaxVolts": "5.0000", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "Core-XYStage": "XYStage", "470 AO-TriggerSampleRate": "100000", "PRIME-Timing-PreTriggerDelayNs": "24672000.0000", "528-DA Device": "528 Shutter", "Focus-Description": "Nikon TE2000 focus adapter", "FilterBlock-State": "5", "520 AO-Demo": "No", "COM3-DelayBetweenCharsMs": "0.0000", "470 Shutter-Volts": "0.0000", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "PRIME-PP 2 THRESHOLD": "125", "PRIME-PP 4 THRESHOLD": "75", "XYStage-Acceleration": "4.0000", "470 Shutter-Demo": "No", "PRIME-Name": "PMPCIECam00", "640-DA Device": "640 Shutter", "405 AO-Block voltage": "false", "ElapsedTime-ms": 1421431, "PRIME-Offset": "100", "445 AO-IOChannel": "Dev1/ao1", "640 AO-MaxVolts": "5.0000", "PositionIndex": 34, "PRIME-PP 1 ENABLED": "Yes", "COM1-BaudRate": "9600", "640-State": "0", "COM3-Verbose": "1", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "LudlController-TransmissionDelay": "0", "PRIME-FullWellCapacity": "62000", "PRIME-SMARTStreamingEnabled": "No", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "640 Shutter-Description": "NI DAC", "528-Description": "DA device that is used as a shutter", "COM4-Description": "Serial port driver (boost:asio)", "405 Shutter-Description": "NI DAC", "TE2000-Name": "TE2000", "528 Shutter-Description": "NI DAC", "COM9-Description": "Serial port driver (boost:asio)", "LudlController-Name": "LudlController", "445 Shutter-TriggerSequenceLength": "1000", "PRIME-SerialNumber": "A18B203008", "445 AO-Volts": "0.0000", "PRIME-X-dimension": "1200", "PRIME-ShutterCloseDelay": "0", "XLIGHT Disk slider-State": "2", "Position": "Default", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 AO-IOChannel": "Dev1/ao5", "520 AO-IOChannel": "Dev1/ao4", "528 AO-TriggerSequenceLength": "1000", "405 Shutter-MaxVolts": "5.0000", "470-DA Device": "470 Shutter", "PRIME-FTCapable": "No", "Core-ChannelGroup": "Channels", "555 AO-SupportsTriggering": "0", "PRIME-Y-dimension": "1200", "Frame": 0, "555 Shutter-Sequenceable": "1", "PRIME-Timing-ExposureTimeNs": "499998000.0000", "Core-ImageProcessor": "", "528-State": "0", "520 AO-Block voltage": "false", "XLIGHT Excitation Wheel-Label": "Excitation-2", "640 Shutter-Demo": "No", "COM3-StopBits": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "520 Shutter-IOChannel": "Dev1/ao11", "Width": 1200, "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-SupportsTriggering": "0", "640 AO-TriggerSampleRate": "100000", "520-Description": "DA device that is used as a shutter", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "405 Shutter-Sequenceable": "1", "405 Shutter-SupportsTriggering": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-TriggerSampleRate": "100000", "640 Shutter-MaxVolts": "5.0000", "555 AO-MaxVolts": "5.0000", "520 AO-TriggerSequenceLength": "1000", "COM3-BaudRate": "9600", "COM9-BaudRate": "9600", "Vincent-D3-Channel #": "Ch. #1", "PRIME-PMode": "Normal", "OpticalPath-Label": "Left(100)", "BitDepth": 16, "555 AO-TriggerSequenceLength": "1000", "445 Shutter-Description": "NI DAC", "405 AO-Sequenceable": "1", "PRIME-AcquisitionMethod": "Callbacks", "555 AO-Name": "AnalogIO", "PRIME-ShutterMode": "Pre-Exposure", "COM3-Handshaking": "Off", "445 AO-SupportsTriggering": "0", "ROI": "0-0-1200-1200", "COM9-Verbose": "1", "PRIME-Color": "OFF", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "PRIME-ShutterOpenDelay": "0", "COM1-Parity": "None", "Slice": 9, "SlicePosition": 40, "COM9-DelayBetweenCharsMs": "0.0000", "555-Name": "DA Shutter", "PIEZO-LudlSingleAxisName": "B", "XLIGHT Emission Wheel-Label": "Empty", "640 Shutter-Name": "AnalogIO", "640 AO-Sequenceable": "1", "520 Shutter-Demo": "No", "528 Shutter-Demo": "No", "470-Name": "DA Shutter", "520-DA Device": "520 Shutter", "XYStage-Description": "Ludl XY stage driver adapter", "445 Shutter-MinVolts": "0.0000", "528 AO-TriggerSampleRate": "100000", "405 AO-MinVolts": "0.0000", "405 Shutter-TriggerSequenceLength": "1000", "Height": 1200, "555 AO-Block voltage": "false", "640 Shutter-Volts": "0.0000", "PixelType": "GRAY16", "445 Shutter-Block voltage": "false", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "520 Shutter-TriggerSequenceLength": "1000", "COM9-Name": "COM9", "Core-SLM": "", "555 AO-Volts": "0.0000", "555 Shutter-Block voltage": "false", "XYStage-Speed": "1800.0000", "520 AO-MaxVolts": "5.0000", "528 Shutter-SupportsTriggering": "0", "COM1-Description": "Serial port driver (boost:asio)", "PRIME-MetadataEnabled": "No", "520 Shutter-MinVolts": "0.0000", "470 Shutter-Sequenceable": "1", "PRIME-PreampDelay": "0", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-MinVolts": "0.0000", "ChannelIndex": 4, "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Core-AutoShutter": "1", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "Exposure-ms": 20, "PRIME-PP 2 ENABLED": "Yes", "640 Shutter-TriggerSampleRate": "100000", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "470 Shutter-IOChannel": "Dev1/ao10", "405 AO-Demo": "No", "PixelSizeUm": 1.1, "470 AO-Demo": "No", "470 Shutter-MaxVolts": "5.0000", "Vincent-D3-Name": "Vincent-D3", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FileName": "img_000000000_P-DIC_009.tif", "LudlController-Reset": "Operate", "PRIME-Exposure": "20.00", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "445-Name": "DA Shutter", "555 Shutter-Name": "AnalogIO", "520 Shutter-TriggerSampleRate": "100000", "445 Shutter-IOChannel": "Dev1/ao9", "PRIME-TransposeCorrection": "0", "XYStage-StepSize": "0.1000", "445-Description": "DA device that is used as a shutter", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 AO-IOChannel": "Dev1/ao7", "520 Shutter-Name": "AnalogIO", "COM3-DataBits": "8", "640 AO-Volts": "0.0000", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "555 Shutter-IOChannel": "Dev1/ao13", "WaitInterval": null, "520 Shutter-Sequenceable": "1", "YPositionUm": 545.6, "445-State": "0", "445-DA Device": "445 Shutter", "PRIME-CircularBufferAutoSize": "ON", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "555 AO-TriggerSampleRate": "100000", "445 Shutter-Volts": "0.0000", "XLIGHT Hub-Port": "COM3", "Core-AutoFocus": "", "COM4-Handshaking": "Off", "528 Shutter-MinVolts": "0.0000", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "445 Shutter-Name": "AnalogIO", "528 Shutter-Sequenceable": "1", "XYStage-TransposeMirrorX": "0", "XYStage-TransposeMirrorY": "0", "PRIME-ReadoutRate": "100MHz 16bit", "XYStage-Name": "Stage", "640 AO-SupportsTriggering": "0", "640-Name": "DA Shutter", "640 Shutter-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "640 AO-Block voltage": "false", "445 Shutter-Sequenceable": "1", "470 AO-MaxVolts": "5.0000", "COM4-BaudRate": "9600", "470-Description": "DA device that is used as a shutter", "520 Shutter-Description": "NI DAC", "PRIME-CCDTemperature": "-14.8500", "Time": "2019-12-19 09:59:51 -0500", "640 AO-TriggerSequenceLength": "1000", "470 AO-Name": "AnalogIO", "XLIGHT Disk slider-Label": "Disk Pos 40um", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "Binning": "1x1", "TE2000-ModelType": "1", "COM3-AnswerTimeout": "500.0000", "555 Shutter-SupportsTriggering": "0", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "TE2000-Port": "COM4", "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Description": "NI DAC", "COM4-AnswerTimeout": "500.0000", "COM1-DelayBetweenCharsMs": "0.0000", "FilterBlock-Label": "Confocal", "445 AO-Name": "AnalogIO", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-Timing-ReadoutTimeNs": "24672000.0000", "528 AO-Demo": "No", "520 AO-Sequenceable": "1", "555-Description": "DA device that is used as a shutter", "405-DA Device": "405 Shutter", "405 AO-TriggerSequenceLength": "1000", "640 AO-MinVolts": "0.0000", "640 Shutter-Block voltage": "false", "FrameIndex": 0, "PRIME-Port": "CMOS", "555 AO-MinVolts": "0.0000", "COM3-Description": "Serial port driver (boost:asio)", "528 AO-Description": "NI DAC", "555 AO-IOChannel": "Dev1/ao6", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "555 AO-TriggerInputLine": "/Dev1/PFI0", "PRIME-ActualInterval-ms": "390.5200", "LudlController-Port": "COM9", "445 Shutter-Demo": "No", "PRIME-Trigger-Expose Out-Mux": "1", "SliceIndex": 9, "PRIME-CCDTemperatureSetPoint": "-15.0000", "405 Shutter-MinVolts": "0.0000", "PRIME-ExposeOutMode": "First Row", "AxisPositions": null, "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Volts": "0.0000", "528-Name": "DA Shutter", "555-State": "0", "XLIGHT Dichroic-Label": "Di03-R635-t1", "445 AO-Sequenceable": "1", "405-Name": "DA Shutter", "405 AO-IOChannel": "Dev1/ao0", "Core-Camera": "PRIME", "Focus-Name": "Focus", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "405 Shutter-IOChannel": "Dev1/ao8", "640 AO-Demo": "No", "640 Shutter-SupportsTriggering": "0", "445 AO-Demo": "No", "555-DA Device": "555 Shutter", "470 Shutter-Description": "NI DAC", "528 AO-Volts": "0.0000", "COM1-DataBits": "8", "Vincent-D3-Address": "x", "520 AO-SupportsTriggering": "0", "405-Description": "DA device that is used as a shutter", "640 Shutter-MinVolts": "0.0000", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "405 AO-Name": "AnalogIO", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "640 AO-TriggerInputLine": "/Dev1/PFI0", "405 AO-TriggerInputLine": "/Dev1/PFI0", "640-Description": "DA device that is used as a shutter", "445 Shutter-TriggerSampleRate": "100000", "470 Shutter-TriggerSequenceLength": "1000", "COM1-StopBits": "1", "520 Shutter-SupportsTriggering": "0", "405-State": "0", "Core-Initialize": "1", "XLIGHT Emission Wheel-State": "3", "520 AO-Description": "NI DAC", "528 AO-TriggerInputLine": "/Dev1/PFI0", "445 Shutter-MaxVolts": "5.0000", "COM9-Handshaking": "Off", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "XLIGHT Spin motor-Label": "On (spinning)", "PRIME-PP 5 ENABLED": "No", "UUID": "2afada7b-3935-4a83-b0f2-49efbd30dc2e", "PRIME-TriggerMode": "Internal Trigger", "COM1-Handshaking": "Off", "PRIME-Timing-PostTriggerDelayNs": "49344000.0000", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "Focus-Position": "17741.5000", "528 Shutter-Block voltage": "false", "Channel": "P-DIC", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "555 Shutter-Demo": "No", "405 Shutter-Demo": "No", "528 AO-Name": "AnalogIO", "520-State": "0", "Core-Galvo": "", "405 Shutter-TriggerSampleRate": "100000", "Focus-FocusStepSize_(nm)": "100", "PRIME-PixelType": "16bit", "COM4-DataBits": "8", "470 AO-IOChannel": "Dev1/ao2", "520 AO-MinVolts": "0.0000", "COM9-Parity": "None", "PRIME-CircularBufferEnabled": "ON", "PRIME-PP 6 Reset": "No", "640 AO-Description": "NI DAC", "Source": "PRIME", "445 AO-MinVolts": "0.0000", "PIEZO-Name": "Stage", "445 AO-Description": "NI DAC", "PRIME-CircularBufferFrameCount": "46", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "520 AO-Volts": "0.0000", "528 Shutter-TriggerSampleRate": "100000", "XPositionUm": -1324.9, "OpticalPath-State": "4", "PRIME-PP 3 THRESHOLD": "80", "PRIME-PVCAM Version": "3.7.1", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Gain": "1-HDR", "640 Shutter-TriggerSequenceLength": "1000", "528 AO-SupportsTriggering": "0", "445 AO-TriggerInputLine": "/Dev1/PFI0", "OpticalPath-Name": "OpticalPath", "PRIME-PP 4 ENABLED": "Yes", "PRIME-PVCAM Adapter Version": "1.3.29", "COM3-Name": "COM3", "405 AO-MaxVolts": "5.0000", "520 Shutter-Block voltage": "false", "PRIME-Timing-ClearingTimeNs": "49344000.0000", "Core-Focus": "PIEZO", "528 AO-Block voltage": "false", "TE2000-Description": "Nikon TE2000 hub", "528 AO-MinVolts": "0.0000", "COM4-Parity": "None", "Summary": { "Slices": 11, "ChContrastMin": [ 0, 0, 0, 0, 0, 0 ], "PixelAspect": 1, "SlicesFirst": true, "Interval_ms": 0, "Time": "2019-12-19 09:59:21 -0500", "Prefix": "seq_13", "InitialPositionList": [ { "Label": "Pos0", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.21], "Focus": [17741.5], "XYStage": [ 44542.200000000004, 31636.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos1", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.22], "Focus": [17741.4], "XYStage": [ 43801.100000000006, 31637.9 ] }, "GridColumnIndex": 0 }, { "Label": "Pos2", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.5], "XYStage": [ 38561.3, 32040.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos3", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.185], "Focus": [17741.3], "XYStage": [ 37698.4, 32041.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos4", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.16], "Focus": [17741.5], "XYStage": [ 32375.4, 31758.9 ] }, "GridColumnIndex": 0 }, { "Label": "Pos5", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.17000000000002], "Focus": [17741.3], "XYStage": [ 31770.7, 31758.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos6", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.4], "XYStage": [ 26536.800000000003, 31068.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos7", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.205], "Focus": [17741.4], "XYStage": [ 25420.2, 31068.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos8", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.85], "Focus": [17741.3], "XYStage": [ 21059, 31508 ] }, "GridColumnIndex": 0 }, { "Label": "Pos9", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.82], "Focus": [17741.3], "XYStage": [ 19731.9, 31506.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos10", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.79], "Focus": [17741.4], "XYStage": [ 14528.5, 32007.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos11", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.83], "Focus": [17741.2], "XYStage": [ 13830.300000000001, 32009.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos12", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.79], "Focus": [17741.3], "XYStage": [ 8775.300000000001, 32017.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos13", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.815], "Focus": [17741.3], "XYStage": [ 8109.200000000001, 32008.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos14", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.805], "Focus": [17741.3], "XYStage": [ 3483.3, 31908.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos15", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.82], "Focus": [17741.2], "XYStage": [ 2686.8, 31903.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos16", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [211.48000000000002], "Focus": [17741.1], "XYStage": [ -1978.9, 31973.600000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos17", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [211.51500000000001], "Focus": [17741.1], "XYStage": [ -2957.8, 31982.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos18", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.195], "Focus": [17741.5], "XYStage": [ 47747.4, -576.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos19", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.23000000000002], "Focus": [17741.6], "XYStage": [ 47053.9, -576.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos20", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.205], "Focus": [17741.4], "XYStage": [ 41871.200000000004, -479.8 ] }, "GridColumnIndex": 0 }, { "Label": "Pos21", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.4], "XYStage": [ 40882.600000000006, -392 ] }, "GridColumnIndex": 0 }, { "Label": "Pos22", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.24], "Focus": [17741.4], "XYStage": [ 35905.6, -135.3 ] }, "GridColumnIndex": 0 }, { "Label": "Pos23", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.195], "Focus": [17741.5], "XYStage": [ 34990.9, -132.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos24", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.46], "Focus": [17741.4], "XYStage": [ 29678.100000000002, -958.1 ] }, "GridColumnIndex": 0 }, { "Label": "Pos25", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.44], "Focus": [17741.5], "XYStage": [ 28983.600000000002, -939.4000000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos26", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.475], "Focus": [17741.5], "XYStage": [ 23019.7, -122 ] }, "GridColumnIndex": 0 }, { "Label": "Pos27", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.44], "Focus": [17741.7], "XYStage": [ 22428, -116.80000000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos28", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.46], "Focus": [17741.7], "XYStage": [ 16364.900000000001, -181.10000000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos29", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.48000000000002], "Focus": [17741.5], "XYStage": [ 15712.1, -180 ] }, "GridColumnIndex": 0 }, { "Label": "Pos30", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.45000000000002], "Focus": [17741.7], "XYStage": [ 10346.1, 77.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos31", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.49], "Focus": [17741.4], "XYStage": [ 9755.5, 85.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos32", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.475], "Focus": [17741.5], "XYStage": [ 4775.5, 293.90000000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos33", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.445], "Focus": [17741.4], "XYStage": [ 3954.9, 293.90000000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos34", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.455], "Focus": [17741.4], "XYStage": [ -1324.9, 545.6 ] }, "GridColumnIndex": 0 }, { "Label": "Pos35", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.445], "Focus": [17741.3], "XYStage": [ -2097, 576.6 ] }, "GridColumnIndex": 0 } ], "z-step_um": 10, "GridRow": 0, "BitDepth": 16, "Depth": 2, "Channels": 6, "Source": "Micro-Manager", "ChColors": [ -16711681, -256, -65281, -1, -1, -16776961 ], "MetadataVersion": 10, "KeepShutterOpenChannels": true, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536, 65536 ], "CustomIntervals_ms": [], "Height": 1200, "MicroManagerVersion": "1.4.23 20180219", "GridColumn": 0, "UUID": "18808e7c-f2e6-47b3-90d8-1d779ce87dbd", "PixelType": "GRAY16", "Width": 1200, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\Yan\\20191218-YC113-S7S8-miseq", "Frames": 1, "Comment": "", "UserName": "Nikon", "PixelSize_um": 1.1, "ROI": [ 0, 0, 1200, 1200 ], "Date": "2019-12-19", "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC", "P-DAPI-high" ], "KeepShutterOpenSlices": true, "IJType": 1, "ComputerName": "ZadorConfocal2", "Positions": 36, "PositionIndex": 34, "CameraTimeout": 20000, "TimeFirst": false }, "640 AO-Name": "AnalogIO", "Vincent-D3-Command": "Close" }, "FrameKey-0-4-10": { "COM4-StopBits": "1", "528 AO-MaxVolts": "5.0000", "XLIGHT Excitation Wheel-State": "1", "470 Shutter-Name": "AnalogIO", "Core-Shutter": "Vincent-D3", "555 AO-Demo": "No", "XLIGHT Dichroic-State": "2", "XYStage-StepSize-Y": "0.1000", "ZPositionUm": 268.45500000000004, "XYStage-StepSize-X": "0.1000", "PRIME-FirmwareVersion": "20.28", "PRIME-ClearCycles": "2", "520-Name": "DA Shutter", "405 Shutter-Name": "AnalogIO", "PIEZO-StepSize": "0.0050", "PRIME-ClearMode": "Pre-Exposure", "470 AO-MinVolts": "0.0000", "405 AO-SupportsTriggering": "1", "CameraChannelIndex": 0, "XYStage-StartSpeed": "500.0000", "COM9-StopBits": "2", "405 AO-Description": "NI DAC", "COM4-DelayBetweenCharsMs": "0.0000", "445 AO-Block voltage": "false", "520 AO-TriggerInputLine": "/Dev1/PFI0", "555 AO-Description": "NI DAC", "520 AO-TriggerSampleRate": "100000", "COM4-Verbose": "1", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM4-Name": "COM4", "405 Shutter-Volts": "0.0000", "Vincent-D3-Port": "COM1", "PRIME-PP 4 MIN ADU AFFECTED": "200", "470 Shutter-MinVolts": "0.0000", "PRIME-PP 3 ENABLED": "Yes", "470 AO-Description": "NI DAC", "PRIME-Description": "PVCAM API device adapter", "520 Shutter-MaxVolts": "5.0000", "520 Shutter-Volts": "0.0000", "528 AO-Sequenceable": "1", "PRIME-Binning": "1x1", "555 Shutter-MaxVolts": "5.0000", "528 Shutter-TriggerSequenceLength": "1000", "405 Shutter-Block voltage": "false", "XLIGHT Spin motor-State": "1", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "Vincent-D3-Time to open (ms)": "35.0000", "Vincent-D3-Time to close (ms)": "35.0000", "445 AO-TriggerSampleRate": "100000", "640 Shutter-IOChannel": "Dev1/ao14", "555 AO-Sequenceable": "1", "520 AO-Name": "AnalogIO", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "470 AO-Block voltage": "false", "COM1-Verbose": "1", "COM9-AnswerTimeout": "10000.0000", "528 Shutter-Name": "AnalogIO", "PRIME-Trigger Timeout (secs)": "10", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "NextFrame": 0, "470 AO-TriggerSequenceLength": "1000", "PRIME-TransposeXY": "0", "445 AO-TriggerSequenceLength": "1000", "COM1-Name": "COM1", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "COM9-DataBits": "8", "COM1-AnswerTimeout": "500.0000", "Camera": "", "TE2000-Version": "V2.50-V2.50-V2.10", "528 Shutter-Volts": "0.0000", "XLIGHT Hub-Name": "XLIGHT Hub", "PIEZO-Description": "Ludl stage driver adapter", "PositionName": "Pos34", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSampleRate": "100000", "PRIME-FanSpeedSetpoint": "High", "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "405 AO-TriggerSampleRate": "100000", "470 Shutter-Block voltage": "false", "470-State": "0", "COM3-Parity": "None", "405 AO-Volts": "0.0000", "PRIME-TransposeMirrorY": "0", "PRIME-ChipName": "GS144BSI", "470 AO-Volts": "0.0000", "PRIME-TransposeMirrorX": "0", "445 Shutter-SupportsTriggering": "0", "470 Shutter-SupportsTriggering": "0", "XLIGHT Hub-Description": "XLIGHT hub", "PRIME-PreampOffLimit": "10000", "470 AO-Sequenceable": "1", "528 Shutter-MaxVolts": "5.0000", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "Core-XYStage": "XYStage", "470 AO-TriggerSampleRate": "100000", "PRIME-Timing-PreTriggerDelayNs": "24672000.0000", "528-DA Device": "528 Shutter", "Focus-Description": "Nikon TE2000 focus adapter", "FilterBlock-State": "5", "520 AO-Demo": "No", "COM3-DelayBetweenCharsMs": "0.0000", "470 Shutter-Volts": "0.0000", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "PRIME-PP 2 THRESHOLD": "125", "PRIME-PP 4 THRESHOLD": "75", "XYStage-Acceleration": "4.0000", "470 Shutter-Demo": "No", "PRIME-Name": "PMPCIECam00", "640-DA Device": "640 Shutter", "405 AO-Block voltage": "false", "ElapsedTime-ms": 1421704, "PRIME-Offset": "100", "445 AO-IOChannel": "Dev1/ao1", "640 AO-MaxVolts": "5.0000", "PositionIndex": 34, "PRIME-PP 1 ENABLED": "Yes", "COM1-BaudRate": "9600", "640-State": "0", "COM3-Verbose": "1", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "LudlController-TransmissionDelay": "0", "PRIME-FullWellCapacity": "62000", "PRIME-SMARTStreamingEnabled": "No", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "640 Shutter-Description": "NI DAC", "528-Description": "DA device that is used as a shutter", "COM4-Description": "Serial port driver (boost:asio)", "405 Shutter-Description": "NI DAC", "TE2000-Name": "TE2000", "528 Shutter-Description": "NI DAC", "COM9-Description": "Serial port driver (boost:asio)", "LudlController-Name": "LudlController", "445 Shutter-TriggerSequenceLength": "1000", "PRIME-SerialNumber": "A18B203008", "445 AO-Volts": "0.0000", "PRIME-X-dimension": "1200", "PRIME-ShutterCloseDelay": "0", "XLIGHT Disk slider-State": "2", "Position": "Default", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 AO-IOChannel": "Dev1/ao5", "520 AO-IOChannel": "Dev1/ao4", "528 AO-TriggerSequenceLength": "1000", "405 Shutter-MaxVolts": "5.0000", "470-DA Device": "470 Shutter", "PRIME-FTCapable": "No", "Core-ChannelGroup": "Channels", "555 AO-SupportsTriggering": "0", "PRIME-Y-dimension": "1200", "Frame": 0, "555 Shutter-Sequenceable": "1", "PRIME-Timing-ExposureTimeNs": "499998000.0000", "Core-ImageProcessor": "", "528-State": "0", "520 AO-Block voltage": "false", "XLIGHT Excitation Wheel-Label": "Excitation-2", "640 Shutter-Demo": "No", "COM3-StopBits": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "520 Shutter-IOChannel": "Dev1/ao11", "Width": 1200, "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-SupportsTriggering": "0", "640 AO-TriggerSampleRate": "100000", "520-Description": "DA device that is used as a shutter", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "405 Shutter-Sequenceable": "1", "405 Shutter-SupportsTriggering": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-TriggerSampleRate": "100000", "640 Shutter-MaxVolts": "5.0000", "555 AO-MaxVolts": "5.0000", "520 AO-TriggerSequenceLength": "1000", "COM3-BaudRate": "9600", "COM9-BaudRate": "9600", "Vincent-D3-Channel #": "Ch. #1", "PRIME-PMode": "Normal", "OpticalPath-Label": "Left(100)", "BitDepth": 16, "555 AO-TriggerSequenceLength": "1000", "445 Shutter-Description": "NI DAC", "405 AO-Sequenceable": "1", "PRIME-AcquisitionMethod": "Callbacks", "555 AO-Name": "AnalogIO", "PRIME-ShutterMode": "Pre-Exposure", "COM3-Handshaking": "Off", "445 AO-SupportsTriggering": "0", "ROI": "0-0-1200-1200", "COM9-Verbose": "1", "PRIME-Color": "OFF", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "PRIME-ShutterOpenDelay": "0", "COM1-Parity": "None", "Slice": 10, "SlicePosition": 50, "COM9-DelayBetweenCharsMs": "0.0000", "555-Name": "DA Shutter", "PIEZO-LudlSingleAxisName": "B", "XLIGHT Emission Wheel-Label": "Empty", "640 Shutter-Name": "AnalogIO", "640 AO-Sequenceable": "1", "520 Shutter-Demo": "No", "528 Shutter-Demo": "No", "470-Name": "DA Shutter", "520-DA Device": "520 Shutter", "XYStage-Description": "Ludl XY stage driver adapter", "445 Shutter-MinVolts": "0.0000", "528 AO-TriggerSampleRate": "100000", "405 AO-MinVolts": "0.0000", "405 Shutter-TriggerSequenceLength": "1000", "Height": 1200, "555 AO-Block voltage": "false", "640 Shutter-Volts": "0.0000", "PixelType": "GRAY16", "445 Shutter-Block voltage": "false", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "520 Shutter-TriggerSequenceLength": "1000", "COM9-Name": "COM9", "Core-SLM": "", "555 AO-Volts": "0.0000", "555 Shutter-Block voltage": "false", "XYStage-Speed": "1800.0000", "520 AO-MaxVolts": "5.0000", "528 Shutter-SupportsTriggering": "0", "COM1-Description": "Serial port driver (boost:asio)", "PRIME-MetadataEnabled": "No", "520 Shutter-MinVolts": "0.0000", "470 Shutter-Sequenceable": "1", "PRIME-PreampDelay": "0", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-MinVolts": "0.0000", "ChannelIndex": 4, "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Core-AutoShutter": "1", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "Exposure-ms": 20, "PRIME-PP 2 ENABLED": "Yes", "640 Shutter-TriggerSampleRate": "100000", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "470 Shutter-IOChannel": "Dev1/ao10", "405 AO-Demo": "No", "PixelSizeUm": 1.1, "470 AO-Demo": "No", "470 Shutter-MaxVolts": "5.0000", "Vincent-D3-Name": "Vincent-D3", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FileName": "img_000000000_P-DIC_010.tif", "LudlController-Reset": "Operate", "PRIME-Exposure": "20.00", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "445-Name": "DA Shutter", "555 Shutter-Name": "AnalogIO", "520 Shutter-TriggerSampleRate": "100000", "445 Shutter-IOChannel": "Dev1/ao9", "PRIME-TransposeCorrection": "0", "XYStage-StepSize": "0.1000", "445-Description": "DA device that is used as a shutter", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 AO-IOChannel": "Dev1/ao7", "520 Shutter-Name": "AnalogIO", "COM3-DataBits": "8", "640 AO-Volts": "0.0000", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "555 Shutter-IOChannel": "Dev1/ao13", "WaitInterval": null, "520 Shutter-Sequenceable": "1", "YPositionUm": 545.6, "445-State": "0", "445-DA Device": "445 Shutter", "PRIME-CircularBufferAutoSize": "ON", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "555 AO-TriggerSampleRate": "100000", "445 Shutter-Volts": "0.0000", "XLIGHT Hub-Port": "COM3", "Core-AutoFocus": "", "COM4-Handshaking": "Off", "528 Shutter-MinVolts": "0.0000", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "445 Shutter-Name": "AnalogIO", "528 Shutter-Sequenceable": "1", "XYStage-TransposeMirrorX": "0", "XYStage-TransposeMirrorY": "0", "PRIME-ReadoutRate": "100MHz 16bit", "XYStage-Name": "Stage", "640 AO-SupportsTriggering": "0", "640-Name": "DA Shutter", "640 Shutter-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "640 AO-Block voltage": "false", "445 Shutter-Sequenceable": "1", "470 AO-MaxVolts": "5.0000", "COM4-BaudRate": "9600", "470-Description": "DA device that is used as a shutter", "520 Shutter-Description": "NI DAC", "PRIME-CCDTemperature": "-14.8500", "Time": "2019-12-19 09:59:52 -0500", "640 AO-TriggerSequenceLength": "1000", "470 AO-Name": "AnalogIO", "XLIGHT Disk slider-Label": "Disk Pos 40um", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "Binning": "1x1", "TE2000-ModelType": "1", "COM3-AnswerTimeout": "500.0000", "555 Shutter-SupportsTriggering": "0", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "TE2000-Port": "COM4", "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Description": "NI DAC", "COM4-AnswerTimeout": "500.0000", "COM1-DelayBetweenCharsMs": "0.0000", "FilterBlock-Label": "Confocal", "445 AO-Name": "AnalogIO", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-Timing-ReadoutTimeNs": "24672000.0000", "528 AO-Demo": "No", "520 AO-Sequenceable": "1", "555-Description": "DA device that is used as a shutter", "405-DA Device": "405 Shutter", "405 AO-TriggerSequenceLength": "1000", "640 AO-MinVolts": "0.0000", "640 Shutter-Block voltage": "false", "FrameIndex": 0, "PRIME-Port": "CMOS", "555 AO-MinVolts": "0.0000", "COM3-Description": "Serial port driver (boost:asio)", "528 AO-Description": "NI DAC", "555 AO-IOChannel": "Dev1/ao6", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "555 AO-TriggerInputLine": "/Dev1/PFI0", "PRIME-ActualInterval-ms": "390.5200", "LudlController-Port": "COM9", "445 Shutter-Demo": "No", "PRIME-Trigger-Expose Out-Mux": "1", "SliceIndex": 10, "PRIME-CCDTemperatureSetPoint": "-15.0000", "405 Shutter-MinVolts": "0.0000", "PRIME-ExposeOutMode": "First Row", "AxisPositions": null, "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Volts": "0.0000", "528-Name": "DA Shutter", "555-State": "0", "XLIGHT Dichroic-Label": "Di03-R635-t1", "445 AO-Sequenceable": "1", "405-Name": "DA Shutter", "405 AO-IOChannel": "Dev1/ao0", "Core-Camera": "PRIME", "Focus-Name": "Focus", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "405 Shutter-IOChannel": "Dev1/ao8", "640 AO-Demo": "No", "640 Shutter-SupportsTriggering": "0", "445 AO-Demo": "No", "555-DA Device": "555 Shutter", "470 Shutter-Description": "NI DAC", "528 AO-Volts": "0.0000", "COM1-DataBits": "8", "Vincent-D3-Address": "x", "520 AO-SupportsTriggering": "0", "405-Description": "DA device that is used as a shutter", "640 Shutter-MinVolts": "0.0000", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "405 AO-Name": "AnalogIO", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "640 AO-TriggerInputLine": "/Dev1/PFI0", "405 AO-TriggerInputLine": "/Dev1/PFI0", "640-Description": "DA device that is used as a shutter", "445 Shutter-TriggerSampleRate": "100000", "470 Shutter-TriggerSequenceLength": "1000", "COM1-StopBits": "1", "520 Shutter-SupportsTriggering": "0", "405-State": "0", "Core-Initialize": "1", "XLIGHT Emission Wheel-State": "3", "520 AO-Description": "NI DAC", "528 AO-TriggerInputLine": "/Dev1/PFI0", "445 Shutter-MaxVolts": "5.0000", "COM9-Handshaking": "Off", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "XLIGHT Spin motor-Label": "On (spinning)", "PRIME-PP 5 ENABLED": "No", "UUID": "bef84eed-f1b6-465f-ae6f-26ee2a52ec64", "PRIME-TriggerMode": "Internal Trigger", "COM1-Handshaking": "Off", "PRIME-Timing-PostTriggerDelayNs": "49344000.0000", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "Focus-Position": "17741.5000", "528 Shutter-Block voltage": "false", "Channel": "P-DIC", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "555 Shutter-Demo": "No", "405 Shutter-Demo": "No", "528 AO-Name": "AnalogIO", "520-State": "0", "Core-Galvo": "", "405 Shutter-TriggerSampleRate": "100000", "Focus-FocusStepSize_(nm)": "100", "PRIME-PixelType": "16bit", "COM4-DataBits": "8", "470 AO-IOChannel": "Dev1/ao2", "520 AO-MinVolts": "0.0000", "COM9-Parity": "None", "PRIME-CircularBufferEnabled": "ON", "PRIME-PP 6 Reset": "No", "640 AO-Description": "NI DAC", "Source": "PRIME", "445 AO-MinVolts": "0.0000", "PIEZO-Name": "Stage", "445 AO-Description": "NI DAC", "PRIME-CircularBufferFrameCount": "46", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "520 AO-Volts": "0.0000", "528 Shutter-TriggerSampleRate": "100000", "XPositionUm": -1324.9, "OpticalPath-State": "4", "PRIME-PP 3 THRESHOLD": "80", "PRIME-PVCAM Version": "3.7.1", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Gain": "1-HDR", "640 Shutter-TriggerSequenceLength": "1000", "528 AO-SupportsTriggering": "0", "445 AO-TriggerInputLine": "/Dev1/PFI0", "OpticalPath-Name": "OpticalPath", "PRIME-PP 4 ENABLED": "Yes", "PRIME-PVCAM Adapter Version": "1.3.29", "COM3-Name": "COM3", "405 AO-MaxVolts": "5.0000", "520 Shutter-Block voltage": "false", "PRIME-Timing-ClearingTimeNs": "49344000.0000", "Core-Focus": "PIEZO", "528 AO-Block voltage": "false", "TE2000-Description": "Nikon TE2000 hub", "528 AO-MinVolts": "0.0000", "COM4-Parity": "None", "Summary": { "Slices": 11, "ChContrastMin": [ 0, 0, 0, 0, 0, 0 ], "PixelAspect": 1, "SlicesFirst": true, "Interval_ms": 0, "Time": "2019-12-19 09:59:21 -0500", "Prefix": "seq_13", "InitialPositionList": [ { "Label": "Pos0", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.21], "Focus": [17741.5], "XYStage": [ 44542.200000000004, 31636.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos1", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.22], "Focus": [17741.4], "XYStage": [ 43801.100000000006, 31637.9 ] }, "GridColumnIndex": 0 }, { "Label": "Pos2", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.5], "XYStage": [ 38561.3, 32040.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos3", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.185], "Focus": [17741.3], "XYStage": [ 37698.4, 32041.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos4", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.16], "Focus": [17741.5], "XYStage": [ 32375.4, 31758.9 ] }, "GridColumnIndex": 0 }, { "Label": "Pos5", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.17000000000002], "Focus": [17741.3], "XYStage": [ 31770.7, 31758.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos6", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.4], "XYStage": [ 26536.800000000003, 31068.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos7", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.205], "Focus": [17741.4], "XYStage": [ 25420.2, 31068.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos8", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.85], "Focus": [17741.3], "XYStage": [ 21059, 31508 ] }, "GridColumnIndex": 0 }, { "Label": "Pos9", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.82], "Focus": [17741.3], "XYStage": [ 19731.9, 31506.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos10", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.79], "Focus": [17741.4], "XYStage": [ 14528.5, 32007.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos11", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.83], "Focus": [17741.2], "XYStage": [ 13830.300000000001, 32009.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos12", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.79], "Focus": [17741.3], "XYStage": [ 8775.300000000001, 32017.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos13", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.815], "Focus": [17741.3], "XYStage": [ 8109.200000000001, 32008.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos14", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.805], "Focus": [17741.3], "XYStage": [ 3483.3, 31908.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos15", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.82], "Focus": [17741.2], "XYStage": [ 2686.8, 31903.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos16", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [211.48000000000002], "Focus": [17741.1], "XYStage": [ -1978.9, 31973.600000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos17", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [211.51500000000001], "Focus": [17741.1], "XYStage": [ -2957.8, 31982.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos18", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.195], "Focus": [17741.5], "XYStage": [ 47747.4, -576.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos19", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.23000000000002], "Focus": [17741.6], "XYStage": [ 47053.9, -576.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos20", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.205], "Focus": [17741.4], "XYStage": [ 41871.200000000004, -479.8 ] }, "GridColumnIndex": 0 }, { "Label": "Pos21", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.4], "XYStage": [ 40882.600000000006, -392 ] }, "GridColumnIndex": 0 }, { "Label": "Pos22", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.24], "Focus": [17741.4], "XYStage": [ 35905.6, -135.3 ] }, "GridColumnIndex": 0 }, { "Label": "Pos23", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.195], "Focus": [17741.5], "XYStage": [ 34990.9, -132.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos24", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.46], "Focus": [17741.4], "XYStage": [ 29678.100000000002, -958.1 ] }, "GridColumnIndex": 0 }, { "Label": "Pos25", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.44], "Focus": [17741.5], "XYStage": [ 28983.600000000002, -939.4000000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos26", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.475], "Focus": [17741.5], "XYStage": [ 23019.7, -122 ] }, "GridColumnIndex": 0 }, { "Label": "Pos27", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.44], "Focus": [17741.7], "XYStage": [ 22428, -116.80000000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos28", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.46], "Focus": [17741.7], "XYStage": [ 16364.900000000001, -181.10000000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos29", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.48000000000002], "Focus": [17741.5], "XYStage": [ 15712.1, -180 ] }, "GridColumnIndex": 0 }, { "Label": "Pos30", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.45000000000002], "Focus": [17741.7], "XYStage": [ 10346.1, 77.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos31", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.49], "Focus": [17741.4], "XYStage": [ 9755.5, 85.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos32", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.475], "Focus": [17741.5], "XYStage": [ 4775.5, 293.90000000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos33", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.445], "Focus": [17741.4], "XYStage": [ 3954.9, 293.90000000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos34", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.455], "Focus": [17741.4], "XYStage": [ -1324.9, 545.6 ] }, "GridColumnIndex": 0 }, { "Label": "Pos35", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.445], "Focus": [17741.3], "XYStage": [ -2097, 576.6 ] }, "GridColumnIndex": 0 } ], "z-step_um": 10, "GridRow": 0, "BitDepth": 16, "Depth": 2, "Channels": 6, "Source": "Micro-Manager", "ChColors": [ -16711681, -256, -65281, -1, -1, -16776961 ], "MetadataVersion": 10, "KeepShutterOpenChannels": true, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536, 65536 ], "CustomIntervals_ms": [], "Height": 1200, "MicroManagerVersion": "1.4.23 20180219", "GridColumn": 0, "UUID": "18808e7c-f2e6-47b3-90d8-1d779ce87dbd", "PixelType": "GRAY16", "Width": 1200, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\Yan\\20191218-YC113-S7S8-miseq", "Frames": 1, "Comment": "", "UserName": "Nikon", "PixelSize_um": 1.1, "ROI": [ 0, 0, 1200, 1200 ], "Date": "2019-12-19", "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC", "P-DAPI-high" ], "KeepShutterOpenSlices": true, "IJType": 1, "ComputerName": "ZadorConfocal2", "Positions": 36, "PositionIndex": 34, "CameraTimeout": 20000, "TimeFirst": false }, "640 AO-Name": "AnalogIO", "Vincent-D3-Command": "Close" }, "FrameKey-0-5-0": { "COM4-StopBits": "1", "528 AO-MaxVolts": "5.0000", "XLIGHT Excitation Wheel-State": "1", "470 Shutter-Name": "AnalogIO", "Core-Shutter": "405", "555 AO-Demo": "No", "XLIGHT Dichroic-State": "2", "XYStage-StepSize-Y": "0.1000", "ZPositionUm": 168.455, "XYStage-StepSize-X": "0.1000", "PRIME-FirmwareVersion": "20.28", "PRIME-ClearCycles": "2", "520-Name": "DA Shutter", "405 Shutter-Name": "AnalogIO", "PIEZO-StepSize": "0.0050", "PRIME-ClearMode": "Pre-Exposure", "470 AO-MinVolts": "0.0000", "405 AO-SupportsTriggering": "1", "CameraChannelIndex": 0, "XYStage-StartSpeed": "500.0000", "COM9-StopBits": "2", "405 AO-Description": "NI DAC", "COM4-DelayBetweenCharsMs": "0.0000", "445 AO-Block voltage": "false", "520 AO-TriggerInputLine": "/Dev1/PFI0", "555 AO-Description": "NI DAC", "520 AO-TriggerSampleRate": "100000", "COM4-Verbose": "1", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM4-Name": "COM4", "405 Shutter-Volts": "5.0000", "Vincent-D3-Port": "COM1", "PRIME-PP 4 MIN ADU AFFECTED": "200", "470 Shutter-MinVolts": "0.0000", "PRIME-PP 3 ENABLED": "Yes", "470 AO-Description": "NI DAC", "PRIME-Description": "PVCAM API device adapter", "520 Shutter-MaxVolts": "5.0000", "520 Shutter-Volts": "0.0000", "528 AO-Sequenceable": "1", "PRIME-Binning": "1x1", "555 Shutter-MaxVolts": "5.0000", "528 Shutter-TriggerSequenceLength": "1000", "405 Shutter-Block voltage": "false", "XLIGHT Spin motor-State": "1", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "Vincent-D3-Time to open (ms)": "35.0000", "Vincent-D3-Time to close (ms)": "35.0000", "445 AO-TriggerSampleRate": "100000", "640 Shutter-IOChannel": "Dev1/ao14", "555 AO-Sequenceable": "1", "520 AO-Name": "AnalogIO", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "470 AO-Block voltage": "false", "COM1-Verbose": "1", "COM9-AnswerTimeout": "10000.0000", "528 Shutter-Name": "AnalogIO", "PRIME-Trigger Timeout (secs)": "10", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "NextFrame": 0, "470 AO-TriggerSequenceLength": "1000", "PRIME-TransposeXY": "0", "445 AO-TriggerSequenceLength": "1000", "COM1-Name": "COM1", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "COM9-DataBits": "8", "COM1-AnswerTimeout": "500.0000", "Camera": "", "TE2000-Version": "V2.50-V2.50-V2.10", "528 Shutter-Volts": "0.0000", "XLIGHT Hub-Name": "XLIGHT Hub", "PIEZO-Description": "Ludl stage driver adapter", "PositionName": "Pos34", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSampleRate": "100000", "PRIME-FanSpeedSetpoint": "High", "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "405 AO-TriggerSampleRate": "100000", "470 Shutter-Block voltage": "false", "470-State": "0", "COM3-Parity": "None", "405 AO-Volts": "4.5000", "PRIME-TransposeMirrorY": "0", "PRIME-ChipName": "GS144BSI", "470 AO-Volts": "0.0000", "PRIME-TransposeMirrorX": "0", "445 Shutter-SupportsTriggering": "0", "470 Shutter-SupportsTriggering": "0", "XLIGHT Hub-Description": "XLIGHT hub", "PRIME-PreampOffLimit": "10000", "470 AO-Sequenceable": "1", "528 Shutter-MaxVolts": "5.0000", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "Core-XYStage": "XYStage", "470 AO-TriggerSampleRate": "100000", "PRIME-Timing-PreTriggerDelayNs": "24672000.0000", "528-DA Device": "528 Shutter", "Focus-Description": "Nikon TE2000 focus adapter", "FilterBlock-State": "5", "520 AO-Demo": "No", "COM3-DelayBetweenCharsMs": "0.0000", "470 Shutter-Volts": "0.0000", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "PRIME-PP 2 THRESHOLD": "125", "PRIME-PP 4 THRESHOLD": "75", "XYStage-Acceleration": "4.0000", "470 Shutter-Demo": "No", "PRIME-Name": "PMPCIECam00", "640-DA Device": "640 Shutter", "405 AO-Block voltage": "false", "ElapsedTime-ms": 1423041, "PRIME-Offset": "100", "445 AO-IOChannel": "Dev1/ao1", "640 AO-MaxVolts": "5.0000", "PositionIndex": 34, "PRIME-PP 1 ENABLED": "Yes", "COM1-BaudRate": "9600", "640-State": "0", "COM3-Verbose": "1", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "LudlController-TransmissionDelay": "0", "PRIME-FullWellCapacity": "62000", "PRIME-SMARTStreamingEnabled": "No", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "640 Shutter-Description": "NI DAC", "528-Description": "DA device that is used as a shutter", "COM4-Description": "Serial port driver (boost:asio)", "405 Shutter-Description": "NI DAC", "TE2000-Name": "TE2000", "528 Shutter-Description": "NI DAC", "COM9-Description": "Serial port driver (boost:asio)", "LudlController-Name": "LudlController", "445 Shutter-TriggerSequenceLength": "1000", "PRIME-SerialNumber": "A18B203008", "445 AO-Volts": "0.0000", "PRIME-X-dimension": "1200", "PRIME-ShutterCloseDelay": "0", "XLIGHT Disk slider-State": "2", "Position": "Default", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 AO-IOChannel": "Dev1/ao5", "520 AO-IOChannel": "Dev1/ao4", "528 AO-TriggerSequenceLength": "1000", "405 Shutter-MaxVolts": "5.0000", "470-DA Device": "470 Shutter", "PRIME-FTCapable": "No", "Core-ChannelGroup": "Channels", "555 AO-SupportsTriggering": "0", "PRIME-Y-dimension": "1200", "Frame": 0, "555 Shutter-Sequenceable": "1", "PRIME-Timing-ExposureTimeNs": "499998000.0000", "Core-ImageProcessor": "", "528-State": "0", "520 AO-Block voltage": "false", "XLIGHT Excitation Wheel-Label": "QUAD", "640 Shutter-Demo": "No", "COM3-StopBits": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "520 Shutter-IOChannel": "Dev1/ao11", "Width": 1200, "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-SupportsTriggering": "0", "640 AO-TriggerSampleRate": "100000", "520-Description": "DA device that is used as a shutter", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "405 Shutter-Sequenceable": "1", "405 Shutter-SupportsTriggering": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-TriggerSampleRate": "100000", "640 Shutter-MaxVolts": "5.0000", "555 AO-MaxVolts": "5.0000", "520 AO-TriggerSequenceLength": "1000", "COM3-BaudRate": "9600", "COM9-BaudRate": "9600", "Vincent-D3-Channel #": "Ch. #1", "PRIME-PMode": "Normal", "OpticalPath-Label": "Left(100)", "BitDepth": 16, "555 AO-TriggerSequenceLength": "1000", "445 Shutter-Description": "NI DAC", "405 AO-Sequenceable": "1", "PRIME-AcquisitionMethod": "Callbacks", "555 AO-Name": "AnalogIO", "PRIME-ShutterMode": "Pre-Exposure", "COM3-Handshaking": "Off", "445 AO-SupportsTriggering": "0", "ROI": "0-0-1200-1200", "COM9-Verbose": "1", "PRIME-Color": "OFF", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "PRIME-ShutterOpenDelay": "0", "COM1-Parity": "None", "Slice": 0, "SlicePosition": -50, "COM9-DelayBetweenCharsMs": "0.0000", "555-Name": "DA Shutter", "PIEZO-LudlSingleAxisName": "B", "XLIGHT Emission Wheel-Label": "QUAD", "640 Shutter-Name": "AnalogIO", "640 AO-Sequenceable": "1", "520 Shutter-Demo": "No", "528 Shutter-Demo": "No", "470-Name": "DA Shutter", "520-DA Device": "520 Shutter", "XYStage-Description": "Ludl XY stage driver adapter", "445 Shutter-MinVolts": "0.0000", "528 AO-TriggerSampleRate": "100000", "405 AO-MinVolts": "0.0000", "405 Shutter-TriggerSequenceLength": "1000", "Height": 1200, "555 AO-Block voltage": "false", "640 Shutter-Volts": "0.0000", "PixelType": "GRAY16", "445 Shutter-Block voltage": "false", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "520 Shutter-TriggerSequenceLength": "1000", "COM9-Name": "COM9", "Core-SLM": "", "555 AO-Volts": "0.0000", "555 Shutter-Block voltage": "false", "XYStage-Speed": "1800.0000", "520 AO-MaxVolts": "5.0000", "528 Shutter-SupportsTriggering": "0", "COM1-Description": "Serial port driver (boost:asio)", "PRIME-MetadataEnabled": "No", "520 Shutter-MinVolts": "0.0000", "470 Shutter-Sequenceable": "1", "PRIME-PreampDelay": "0", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-MinVolts": "0.0000", "ChannelIndex": 5, "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Core-AutoShutter": "1", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "Exposure-ms": 300, "PRIME-PP 2 ENABLED": "Yes", "640 Shutter-TriggerSampleRate": "100000", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "470 Shutter-IOChannel": "Dev1/ao10", "405 AO-Demo": "No", "PixelSizeUm": 1.1, "470 AO-Demo": "No", "470 Shutter-MaxVolts": "5.0000", "Vincent-D3-Name": "Vincent-D3", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FileName": "img_000000000_P-DAPI-high_000.tif", "LudlController-Reset": "Operate", "PRIME-Exposure": "300.00", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "445-Name": "DA Shutter", "555 Shutter-Name": "AnalogIO", "520 Shutter-TriggerSampleRate": "100000", "445 Shutter-IOChannel": "Dev1/ao9", "PRIME-TransposeCorrection": "0", "XYStage-StepSize": "0.1000", "445-Description": "DA device that is used as a shutter", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 AO-IOChannel": "Dev1/ao7", "520 Shutter-Name": "AnalogIO", "COM3-DataBits": "8", "640 AO-Volts": "0.0000", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "555 Shutter-IOChannel": "Dev1/ao13", "WaitInterval": null, "520 Shutter-Sequenceable": "1", "YPositionUm": 545.6, "445-State": "0", "445-DA Device": "445 Shutter", "PRIME-CircularBufferAutoSize": "ON", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "555 AO-TriggerSampleRate": "100000", "445 Shutter-Volts": "0.0000", "XLIGHT Hub-Port": "COM3", "Core-AutoFocus": "", "COM4-Handshaking": "Off", "528 Shutter-MinVolts": "0.0000", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "445 Shutter-Name": "AnalogIO", "528 Shutter-Sequenceable": "1", "XYStage-TransposeMirrorX": "0", "XYStage-TransposeMirrorY": "0", "PRIME-ReadoutRate": "100MHz 16bit", "XYStage-Name": "Stage", "640 AO-SupportsTriggering": "0", "640-Name": "DA Shutter", "640 Shutter-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "640 AO-Block voltage": "false", "445 Shutter-Sequenceable": "1", "470 AO-MaxVolts": "5.0000", "COM4-BaudRate": "9600", "470-Description": "DA device that is used as a shutter", "520 Shutter-Description": "NI DAC", "PRIME-CCDTemperature": "-14.8500", "Time": "2019-12-19 09:59:53 -0500", "640 AO-TriggerSequenceLength": "1000", "470 AO-Name": "AnalogIO", "XLIGHT Disk slider-Label": "Disk Pos 40um", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "Binning": "1x1", "TE2000-ModelType": "1", "COM3-AnswerTimeout": "500.0000", "555 Shutter-SupportsTriggering": "0", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "TE2000-Port": "COM4", "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Description": "NI DAC", "COM4-AnswerTimeout": "500.0000", "COM1-DelayBetweenCharsMs": "0.0000", "FilterBlock-Label": "Confocal", "445 AO-Name": "AnalogIO", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-Timing-ReadoutTimeNs": "24672000.0000", "528 AO-Demo": "No", "520 AO-Sequenceable": "1", "555-Description": "DA device that is used as a shutter", "405-DA Device": "405 Shutter", "405 AO-TriggerSequenceLength": "1000", "640 AO-MinVolts": "0.0000", "640 Shutter-Block voltage": "false", "FrameIndex": 0, "PRIME-Port": "CMOS", "555 AO-MinVolts": "0.0000", "COM3-Description": "Serial port driver (boost:asio)", "528 AO-Description": "NI DAC", "555 AO-IOChannel": "Dev1/ao6", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "555 AO-TriggerInputLine": "/Dev1/PFI0", "PRIME-ActualInterval-ms": "390.5200", "LudlController-Port": "COM9", "445 Shutter-Demo": "No", "PRIME-Trigger-Expose Out-Mux": "1", "SliceIndex": 0, "PRIME-CCDTemperatureSetPoint": "-15.0000", "405 Shutter-MinVolts": "0.0000", "PRIME-ExposeOutMode": "First Row", "AxisPositions": null, "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Volts": "0.0000", "528-Name": "DA Shutter", "555-State": "0", "XLIGHT Dichroic-Label": "QUAD", "445 AO-Sequenceable": "1", "405-Name": "DA Shutter", "405 AO-IOChannel": "Dev1/ao0", "Core-Camera": "PRIME", "Focus-Name": "Focus", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "405 Shutter-IOChannel": "Dev1/ao8", "640 AO-Demo": "No", "640 Shutter-SupportsTriggering": "0", "445 AO-Demo": "No", "555-DA Device": "555 Shutter", "470 Shutter-Description": "NI DAC", "528 AO-Volts": "0.0000", "COM1-DataBits": "8", "Vincent-D3-Address": "x", "520 AO-SupportsTriggering": "0", "405-Description": "DA device that is used as a shutter", "640 Shutter-MinVolts": "0.0000", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "405 AO-Name": "AnalogIO", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "640 AO-TriggerInputLine": "/Dev1/PFI0", "405 AO-TriggerInputLine": "/Dev1/PFI0", "640-Description": "DA device that is used as a shutter", "445 Shutter-TriggerSampleRate": "100000", "470 Shutter-TriggerSequenceLength": "1000", "COM1-StopBits": "1", "520 Shutter-SupportsTriggering": "0", "405-State": "1", "Core-Initialize": "1", "XLIGHT Emission Wheel-State": "3", "520 AO-Description": "NI DAC", "528 AO-TriggerInputLine": "/Dev1/PFI0", "445 Shutter-MaxVolts": "5.0000", "COM9-Handshaking": "Off", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "XLIGHT Spin motor-Label": "On (spinning)", "PRIME-PP 5 ENABLED": "No", "UUID": "357c6369-7058-469e-871f-0019e0cc884f", "PRIME-TriggerMode": "Internal Trigger", "COM1-Handshaking": "Off", "PRIME-Timing-PostTriggerDelayNs": "49344000.0000", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "Focus-Position": "17741.5000", "528 Shutter-Block voltage": "false", "Channel": "P-DAPI-high", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "555 Shutter-Demo": "No", "405 Shutter-Demo": "No", "528 AO-Name": "AnalogIO", "520-State": "0", "Core-Galvo": "", "405 Shutter-TriggerSampleRate": "100000", "Focus-FocusStepSize_(nm)": "100", "PRIME-PixelType": "16bit", "COM4-DataBits": "8", "470 AO-IOChannel": "Dev1/ao2", "520 AO-MinVolts": "0.0000", "COM9-Parity": "None", "PRIME-CircularBufferEnabled": "ON", "PRIME-PP 6 Reset": "No", "640 AO-Description": "NI DAC", "Source": "PRIME", "445 AO-MinVolts": "0.0000", "PIEZO-Name": "Stage", "445 AO-Description": "NI DAC", "PRIME-CircularBufferFrameCount": "46", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "520 AO-Volts": "0.0000", "528 Shutter-TriggerSampleRate": "100000", "XPositionUm": -1324.9, "OpticalPath-State": "4", "PRIME-PP 3 THRESHOLD": "80", "PRIME-PVCAM Version": "3.7.1", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Gain": "1-HDR", "640 Shutter-TriggerSequenceLength": "1000", "528 AO-SupportsTriggering": "0", "445 AO-TriggerInputLine": "/Dev1/PFI0", "OpticalPath-Name": "OpticalPath", "PRIME-PP 4 ENABLED": "Yes", "PRIME-PVCAM Adapter Version": "1.3.29", "COM3-Name": "COM3", "405 AO-MaxVolts": "5.0000", "520 Shutter-Block voltage": "false", "PRIME-Timing-ClearingTimeNs": "49344000.0000", "Core-Focus": "PIEZO", "528 AO-Block voltage": "false", "TE2000-Description": "Nikon TE2000 hub", "528 AO-MinVolts": "0.0000", "COM4-Parity": "None", "Summary": { "Slices": 11, "ChContrastMin": [ 0, 0, 0, 0, 0, 0 ], "PixelAspect": 1, "SlicesFirst": true, "Interval_ms": 0, "Time": "2019-12-19 09:59:21 -0500", "Prefix": "seq_13", "InitialPositionList": [ { "Label": "Pos0", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.21], "Focus": [17741.5], "XYStage": [ 44542.200000000004, 31636.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos1", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.22], "Focus": [17741.4], "XYStage": [ 43801.100000000006, 31637.9 ] }, "GridColumnIndex": 0 }, { "Label": "Pos2", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.5], "XYStage": [ 38561.3, 32040.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos3", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.185], "Focus": [17741.3], "XYStage": [ 37698.4, 32041.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos4", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.16], "Focus": [17741.5], "XYStage": [ 32375.4, 31758.9 ] }, "GridColumnIndex": 0 }, { "Label": "Pos5", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.17000000000002], "Focus": [17741.3], "XYStage": [ 31770.7, 31758.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos6", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.4], "XYStage": [ 26536.800000000003, 31068.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos7", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.205], "Focus": [17741.4], "XYStage": [ 25420.2, 31068.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos8", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.85], "Focus": [17741.3], "XYStage": [ 21059, 31508 ] }, "GridColumnIndex": 0 }, { "Label": "Pos9", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.82], "Focus": [17741.3], "XYStage": [ 19731.9, 31506.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos10", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.79], "Focus": [17741.4], "XYStage": [ 14528.5, 32007.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos11", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.83], "Focus": [17741.2], "XYStage": [ 13830.300000000001, 32009.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos12", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.79], "Focus": [17741.3], "XYStage": [ 8775.300000000001, 32017.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos13", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.815], "Focus": [17741.3], "XYStage": [ 8109.200000000001, 32008.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos14", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.805], "Focus": [17741.3], "XYStage": [ 3483.3, 31908.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos15", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.82], "Focus": [17741.2], "XYStage": [ 2686.8, 31903.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos16", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [211.48000000000002], "Focus": [17741.1], "XYStage": [ -1978.9, 31973.600000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos17", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [211.51500000000001], "Focus": [17741.1], "XYStage": [ -2957.8, 31982.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos18", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.195], "Focus": [17741.5], "XYStage": [ 47747.4, -576.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos19", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.23000000000002], "Focus": [17741.6], "XYStage": [ 47053.9, -576.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos20", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.205], "Focus": [17741.4], "XYStage": [ 41871.200000000004, -479.8 ] }, "GridColumnIndex": 0 }, { "Label": "Pos21", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.4], "XYStage": [ 40882.600000000006, -392 ] }, "GridColumnIndex": 0 }, { "Label": "Pos22", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.24], "Focus": [17741.4], "XYStage": [ 35905.6, -135.3 ] }, "GridColumnIndex": 0 }, { "Label": "Pos23", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.195], "Focus": [17741.5], "XYStage": [ 34990.9, -132.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos24", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.46], "Focus": [17741.4], "XYStage": [ 29678.100000000002, -958.1 ] }, "GridColumnIndex": 0 }, { "Label": "Pos25", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.44], "Focus": [17741.5], "XYStage": [ 28983.600000000002, -939.4000000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos26", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.475], "Focus": [17741.5], "XYStage": [ 23019.7, -122 ] }, "GridColumnIndex": 0 }, { "Label": "Pos27", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.44], "Focus": [17741.7], "XYStage": [ 22428, -116.80000000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos28", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.46], "Focus": [17741.7], "XYStage": [ 16364.900000000001, -181.10000000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos29", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.48000000000002], "Focus": [17741.5], "XYStage": [ 15712.1, -180 ] }, "GridColumnIndex": 0 }, { "Label": "Pos30", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.45000000000002], "Focus": [17741.7], "XYStage": [ 10346.1, 77.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos31", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.49], "Focus": [17741.4], "XYStage": [ 9755.5, 85.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos32", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.475], "Focus": [17741.5], "XYStage": [ 4775.5, 293.90000000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos33", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.445], "Focus": [17741.4], "XYStage": [ 3954.9, 293.90000000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos34", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.455], "Focus": [17741.4], "XYStage": [ -1324.9, 545.6 ] }, "GridColumnIndex": 0 }, { "Label": "Pos35", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.445], "Focus": [17741.3], "XYStage": [ -2097, 576.6 ] }, "GridColumnIndex": 0 } ], "z-step_um": 10, "GridRow": 0, "BitDepth": 16, "Depth": 2, "Channels": 6, "Source": "Micro-Manager", "ChColors": [ -16711681, -256, -65281, -1, -1, -16776961 ], "MetadataVersion": 10, "KeepShutterOpenChannels": true, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536, 65536 ], "CustomIntervals_ms": [], "Height": 1200, "MicroManagerVersion": "1.4.23 20180219", "GridColumn": 0, "UUID": "18808e7c-f2e6-47b3-90d8-1d779ce87dbd", "PixelType": "GRAY16", "Width": 1200, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\Yan\\20191218-YC113-S7S8-miseq", "Frames": 1, "Comment": "", "UserName": "Nikon", "PixelSize_um": 1.1, "ROI": [ 0, 0, 1200, 1200 ], "Date": "2019-12-19", "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC", "P-DAPI-high" ], "KeepShutterOpenSlices": true, "IJType": 1, "ComputerName": "ZadorConfocal2", "Positions": 36, "PositionIndex": 34, "CameraTimeout": 20000, "TimeFirst": false }, "640 AO-Name": "AnalogIO", "Vincent-D3-Command": "Close" }, "FrameKey-0-5-1": { "COM4-StopBits": "1", "528 AO-MaxVolts": "5.0000", "XLIGHT Excitation Wheel-State": "1", "470 Shutter-Name": "AnalogIO", "Core-Shutter": "405", "555 AO-Demo": "No", "XLIGHT Dichroic-State": "2", "XYStage-StepSize-Y": "0.1000", "ZPositionUm": 178.455, "XYStage-StepSize-X": "0.1000", "PRIME-FirmwareVersion": "20.28", "PRIME-ClearCycles": "2", "520-Name": "DA Shutter", "405 Shutter-Name": "AnalogIO", "PIEZO-StepSize": "0.0050", "PRIME-ClearMode": "Pre-Exposure", "470 AO-MinVolts": "0.0000", "405 AO-SupportsTriggering": "1", "CameraChannelIndex": 0, "XYStage-StartSpeed": "500.0000", "COM9-StopBits": "2", "405 AO-Description": "NI DAC", "COM4-DelayBetweenCharsMs": "0.0000", "445 AO-Block voltage": "false", "520 AO-TriggerInputLine": "/Dev1/PFI0", "555 AO-Description": "NI DAC", "520 AO-TriggerSampleRate": "100000", "COM4-Verbose": "1", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM4-Name": "COM4", "405 Shutter-Volts": "5.0000", "Vincent-D3-Port": "COM1", "PRIME-PP 4 MIN ADU AFFECTED": "200", "470 Shutter-MinVolts": "0.0000", "PRIME-PP 3 ENABLED": "Yes", "470 AO-Description": "NI DAC", "PRIME-Description": "PVCAM API device adapter", "520 Shutter-MaxVolts": "5.0000", "520 Shutter-Volts": "0.0000", "528 AO-Sequenceable": "1", "PRIME-Binning": "1x1", "555 Shutter-MaxVolts": "5.0000", "528 Shutter-TriggerSequenceLength": "1000", "405 Shutter-Block voltage": "false", "XLIGHT Spin motor-State": "1", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "Vincent-D3-Time to open (ms)": "35.0000", "Vincent-D3-Time to close (ms)": "35.0000", "445 AO-TriggerSampleRate": "100000", "640 Shutter-IOChannel": "Dev1/ao14", "555 AO-Sequenceable": "1", "520 AO-Name": "AnalogIO", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "470 AO-Block voltage": "false", "COM1-Verbose": "1", "COM9-AnswerTimeout": "10000.0000", "528 Shutter-Name": "AnalogIO", "PRIME-Trigger Timeout (secs)": "10", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "NextFrame": 0, "470 AO-TriggerSequenceLength": "1000", "PRIME-TransposeXY": "0", "445 AO-TriggerSequenceLength": "1000", "COM1-Name": "COM1", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "COM9-DataBits": "8", "COM1-AnswerTimeout": "500.0000", "Camera": "", "TE2000-Version": "V2.50-V2.50-V2.10", "528 Shutter-Volts": "0.0000", "XLIGHT Hub-Name": "XLIGHT Hub", "PIEZO-Description": "Ludl stage driver adapter", "PositionName": "Pos34", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSampleRate": "100000", "PRIME-FanSpeedSetpoint": "High", "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "405 AO-TriggerSampleRate": "100000", "470 Shutter-Block voltage": "false", "470-State": "0", "COM3-Parity": "None", "405 AO-Volts": "4.5000", "PRIME-TransposeMirrorY": "0", "PRIME-ChipName": "GS144BSI", "470 AO-Volts": "0.0000", "PRIME-TransposeMirrorX": "0", "445 Shutter-SupportsTriggering": "0", "470 Shutter-SupportsTriggering": "0", "XLIGHT Hub-Description": "XLIGHT hub", "PRIME-PreampOffLimit": "10000", "470 AO-Sequenceable": "1", "528 Shutter-MaxVolts": "5.0000", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "Core-XYStage": "XYStage", "470 AO-TriggerSampleRate": "100000", "PRIME-Timing-PreTriggerDelayNs": "24672000.0000", "528-DA Device": "528 Shutter", "Focus-Description": "Nikon TE2000 focus adapter", "FilterBlock-State": "5", "520 AO-Demo": "No", "COM3-DelayBetweenCharsMs": "0.0000", "470 Shutter-Volts": "0.0000", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "PRIME-PP 2 THRESHOLD": "125", "PRIME-PP 4 THRESHOLD": "75", "XYStage-Acceleration": "4.0000", "470 Shutter-Demo": "No", "PRIME-Name": "PMPCIECam00", "640-DA Device": "640 Shutter", "405 AO-Block voltage": "false", "ElapsedTime-ms": 1423633, "PRIME-Offset": "100", "445 AO-IOChannel": "Dev1/ao1", "640 AO-MaxVolts": "5.0000", "PositionIndex": 34, "PRIME-PP 1 ENABLED": "Yes", "COM1-BaudRate": "9600", "640-State": "0", "COM3-Verbose": "1", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "LudlController-TransmissionDelay": "0", "PRIME-FullWellCapacity": "62000", "PRIME-SMARTStreamingEnabled": "No", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "640 Shutter-Description": "NI DAC", "528-Description": "DA device that is used as a shutter", "COM4-Description": "Serial port driver (boost:asio)", "405 Shutter-Description": "NI DAC", "TE2000-Name": "TE2000", "528 Shutter-Description": "NI DAC", "COM9-Description": "Serial port driver (boost:asio)", "LudlController-Name": "LudlController", "445 Shutter-TriggerSequenceLength": "1000", "PRIME-SerialNumber": "A18B203008", "445 AO-Volts": "0.0000", "PRIME-X-dimension": "1200", "PRIME-ShutterCloseDelay": "0", "XLIGHT Disk slider-State": "2", "Position": "Default", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 AO-IOChannel": "Dev1/ao5", "520 AO-IOChannel": "Dev1/ao4", "528 AO-TriggerSequenceLength": "1000", "405 Shutter-MaxVolts": "5.0000", "470-DA Device": "470 Shutter", "PRIME-FTCapable": "No", "Core-ChannelGroup": "Channels", "555 AO-SupportsTriggering": "0", "PRIME-Y-dimension": "1200", "Frame": 0, "555 Shutter-Sequenceable": "1", "PRIME-Timing-ExposureTimeNs": "499998000.0000", "Core-ImageProcessor": "", "528-State": "0", "520 AO-Block voltage": "false", "XLIGHT Excitation Wheel-Label": "QUAD", "640 Shutter-Demo": "No", "COM3-StopBits": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "520 Shutter-IOChannel": "Dev1/ao11", "Width": 1200, "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-SupportsTriggering": "0", "640 AO-TriggerSampleRate": "100000", "520-Description": "DA device that is used as a shutter", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "405 Shutter-Sequenceable": "1", "405 Shutter-SupportsTriggering": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-TriggerSampleRate": "100000", "640 Shutter-MaxVolts": "5.0000", "555 AO-MaxVolts": "5.0000", "520 AO-TriggerSequenceLength": "1000", "COM3-BaudRate": "9600", "COM9-BaudRate": "9600", "Vincent-D3-Channel #": "Ch. #1", "PRIME-PMode": "Normal", "OpticalPath-Label": "Left(100)", "BitDepth": 16, "555 AO-TriggerSequenceLength": "1000", "445 Shutter-Description": "NI DAC", "405 AO-Sequenceable": "1", "PRIME-AcquisitionMethod": "Callbacks", "555 AO-Name": "AnalogIO", "PRIME-ShutterMode": "Pre-Exposure", "COM3-Handshaking": "Off", "445 AO-SupportsTriggering": "0", "ROI": "0-0-1200-1200", "COM9-Verbose": "1", "PRIME-Color": "OFF", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "PRIME-ShutterOpenDelay": "0", "COM1-Parity": "None", "Slice": 1, "SlicePosition": -40, "COM9-DelayBetweenCharsMs": "0.0000", "555-Name": "DA Shutter", "PIEZO-LudlSingleAxisName": "B", "XLIGHT Emission Wheel-Label": "QUAD", "640 Shutter-Name": "AnalogIO", "640 AO-Sequenceable": "1", "520 Shutter-Demo": "No", "528 Shutter-Demo": "No", "470-Name": "DA Shutter", "520-DA Device": "520 Shutter", "XYStage-Description": "Ludl XY stage driver adapter", "445 Shutter-MinVolts": "0.0000", "528 AO-TriggerSampleRate": "100000", "405 AO-MinVolts": "0.0000", "405 Shutter-TriggerSequenceLength": "1000", "Height": 1200, "555 AO-Block voltage": "false", "640 Shutter-Volts": "0.0000", "PixelType": "GRAY16", "445 Shutter-Block voltage": "false", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "520 Shutter-TriggerSequenceLength": "1000", "COM9-Name": "COM9", "Core-SLM": "", "555 AO-Volts": "0.0000", "555 Shutter-Block voltage": "false", "XYStage-Speed": "1800.0000", "520 AO-MaxVolts": "5.0000", "528 Shutter-SupportsTriggering": "0", "COM1-Description": "Serial port driver (boost:asio)", "PRIME-MetadataEnabled": "No", "520 Shutter-MinVolts": "0.0000", "470 Shutter-Sequenceable": "1", "PRIME-PreampDelay": "0", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-MinVolts": "0.0000", "ChannelIndex": 5, "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Core-AutoShutter": "1", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "Exposure-ms": 300, "PRIME-PP 2 ENABLED": "Yes", "640 Shutter-TriggerSampleRate": "100000", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "470 Shutter-IOChannel": "Dev1/ao10", "405 AO-Demo": "No", "PixelSizeUm": 1.1, "470 AO-Demo": "No", "470 Shutter-MaxVolts": "5.0000", "Vincent-D3-Name": "Vincent-D3", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FileName": "img_000000000_P-DAPI-high_001.tif", "LudlController-Reset": "Operate", "PRIME-Exposure": "300.00", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "445-Name": "DA Shutter", "555 Shutter-Name": "AnalogIO", "520 Shutter-TriggerSampleRate": "100000", "445 Shutter-IOChannel": "Dev1/ao9", "PRIME-TransposeCorrection": "0", "XYStage-StepSize": "0.1000", "445-Description": "DA device that is used as a shutter", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 AO-IOChannel": "Dev1/ao7", "520 Shutter-Name": "AnalogIO", "COM3-DataBits": "8", "640 AO-Volts": "0.0000", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "555 Shutter-IOChannel": "Dev1/ao13", "WaitInterval": null, "520 Shutter-Sequenceable": "1", "YPositionUm": 545.6, "445-State": "0", "445-DA Device": "445 Shutter", "PRIME-CircularBufferAutoSize": "ON", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "555 AO-TriggerSampleRate": "100000", "445 Shutter-Volts": "0.0000", "XLIGHT Hub-Port": "COM3", "Core-AutoFocus": "", "COM4-Handshaking": "Off", "528 Shutter-MinVolts": "0.0000", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "445 Shutter-Name": "AnalogIO", "528 Shutter-Sequenceable": "1", "XYStage-TransposeMirrorX": "0", "XYStage-TransposeMirrorY": "0", "PRIME-ReadoutRate": "100MHz 16bit", "XYStage-Name": "Stage", "640 AO-SupportsTriggering": "0", "640-Name": "DA Shutter", "640 Shutter-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "640 AO-Block voltage": "false", "445 Shutter-Sequenceable": "1", "470 AO-MaxVolts": "5.0000", "COM4-BaudRate": "9600", "470-Description": "DA device that is used as a shutter", "520 Shutter-Description": "NI DAC", "PRIME-CCDTemperature": "-14.8500", "Time": "2019-12-19 09:59:54 -0500", "640 AO-TriggerSequenceLength": "1000", "470 AO-Name": "AnalogIO", "XLIGHT Disk slider-Label": "Disk Pos 40um", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "Binning": "1x1", "TE2000-ModelType": "1", "COM3-AnswerTimeout": "500.0000", "555 Shutter-SupportsTriggering": "0", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "TE2000-Port": "COM4", "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Description": "NI DAC", "COM4-AnswerTimeout": "500.0000", "COM1-DelayBetweenCharsMs": "0.0000", "FilterBlock-Label": "Confocal", "445 AO-Name": "AnalogIO", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-Timing-ReadoutTimeNs": "24672000.0000", "528 AO-Demo": "No", "520 AO-Sequenceable": "1", "555-Description": "DA device that is used as a shutter", "405-DA Device": "405 Shutter", "405 AO-TriggerSequenceLength": "1000", "640 AO-MinVolts": "0.0000", "640 Shutter-Block voltage": "false", "FrameIndex": 0, "PRIME-Port": "CMOS", "555 AO-MinVolts": "0.0000", "COM3-Description": "Serial port driver (boost:asio)", "528 AO-Description": "NI DAC", "555 AO-IOChannel": "Dev1/ao6", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "555 AO-TriggerInputLine": "/Dev1/PFI0", "PRIME-ActualInterval-ms": "390.5200", "LudlController-Port": "COM9", "445 Shutter-Demo": "No", "PRIME-Trigger-Expose Out-Mux": "1", "SliceIndex": 1, "PRIME-CCDTemperatureSetPoint": "-15.0000", "405 Shutter-MinVolts": "0.0000", "PRIME-ExposeOutMode": "First Row", "AxisPositions": null, "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Volts": "0.0000", "528-Name": "DA Shutter", "555-State": "0", "XLIGHT Dichroic-Label": "QUAD", "445 AO-Sequenceable": "1", "405-Name": "DA Shutter", "405 AO-IOChannel": "Dev1/ao0", "Core-Camera": "PRIME", "Focus-Name": "Focus", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "405 Shutter-IOChannel": "Dev1/ao8", "640 AO-Demo": "No", "640 Shutter-SupportsTriggering": "0", "445 AO-Demo": "No", "555-DA Device": "555 Shutter", "470 Shutter-Description": "NI DAC", "528 AO-Volts": "0.0000", "COM1-DataBits": "8", "Vincent-D3-Address": "x", "520 AO-SupportsTriggering": "0", "405-Description": "DA device that is used as a shutter", "640 Shutter-MinVolts": "0.0000", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "405 AO-Name": "AnalogIO", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "640 AO-TriggerInputLine": "/Dev1/PFI0", "405 AO-TriggerInputLine": "/Dev1/PFI0", "640-Description": "DA device that is used as a shutter", "445 Shutter-TriggerSampleRate": "100000", "470 Shutter-TriggerSequenceLength": "1000", "COM1-StopBits": "1", "520 Shutter-SupportsTriggering": "0", "405-State": "1", "Core-Initialize": "1", "XLIGHT Emission Wheel-State": "3", "520 AO-Description": "NI DAC", "528 AO-TriggerInputLine": "/Dev1/PFI0", "445 Shutter-MaxVolts": "5.0000", "COM9-Handshaking": "Off", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "XLIGHT Spin motor-Label": "On (spinning)", "PRIME-PP 5 ENABLED": "No", "UUID": "2eb72f85-f68e-48e7-8338-9bf7376fac56", "PRIME-TriggerMode": "Internal Trigger", "COM1-Handshaking": "Off", "PRIME-Timing-PostTriggerDelayNs": "49344000.0000", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "Focus-Position": "17741.5000", "528 Shutter-Block voltage": "false", "Channel": "P-DAPI-high", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "555 Shutter-Demo": "No", "405 Shutter-Demo": "No", "528 AO-Name": "AnalogIO", "520-State": "0", "Core-Galvo": "", "405 Shutter-TriggerSampleRate": "100000", "Focus-FocusStepSize_(nm)": "100", "PRIME-PixelType": "16bit", "COM4-DataBits": "8", "470 AO-IOChannel": "Dev1/ao2", "520 AO-MinVolts": "0.0000", "COM9-Parity": "None", "PRIME-CircularBufferEnabled": "ON", "PRIME-PP 6 Reset": "No", "640 AO-Description": "NI DAC", "Source": "PRIME", "445 AO-MinVolts": "0.0000", "PIEZO-Name": "Stage", "445 AO-Description": "NI DAC", "PRIME-CircularBufferFrameCount": "46", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "520 AO-Volts": "0.0000", "528 Shutter-TriggerSampleRate": "100000", "XPositionUm": -1324.9, "OpticalPath-State": "4", "PRIME-PP 3 THRESHOLD": "80", "PRIME-PVCAM Version": "3.7.1", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Gain": "1-HDR", "640 Shutter-TriggerSequenceLength": "1000", "528 AO-SupportsTriggering": "0", "445 AO-TriggerInputLine": "/Dev1/PFI0", "OpticalPath-Name": "OpticalPath", "PRIME-PP 4 ENABLED": "Yes", "PRIME-PVCAM Adapter Version": "1.3.29", "COM3-Name": "COM3", "405 AO-MaxVolts": "5.0000", "520 Shutter-Block voltage": "false", "PRIME-Timing-ClearingTimeNs": "49344000.0000", "Core-Focus": "PIEZO", "528 AO-Block voltage": "false", "TE2000-Description": "Nikon TE2000 hub", "528 AO-MinVolts": "0.0000", "COM4-Parity": "None", "Summary": { "Slices": 11, "ChContrastMin": [ 0, 0, 0, 0, 0, 0 ], "PixelAspect": 1, "SlicesFirst": true, "Interval_ms": 0, "Time": "2019-12-19 09:59:21 -0500", "Prefix": "seq_13", "InitialPositionList": [ { "Label": "Pos0", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.21], "Focus": [17741.5], "XYStage": [ 44542.200000000004, 31636.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos1", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.22], "Focus": [17741.4], "XYStage": [ 43801.100000000006, 31637.9 ] }, "GridColumnIndex": 0 }, { "Label": "Pos2", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.5], "XYStage": [ 38561.3, 32040.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos3", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.185], "Focus": [17741.3], "XYStage": [ 37698.4, 32041.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos4", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.16], "Focus": [17741.5], "XYStage": [ 32375.4, 31758.9 ] }, "GridColumnIndex": 0 }, { "Label": "Pos5", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.17000000000002], "Focus": [17741.3], "XYStage": [ 31770.7, 31758.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos6", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.4], "XYStage": [ 26536.800000000003, 31068.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos7", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.205], "Focus": [17741.4], "XYStage": [ 25420.2, 31068.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos8", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.85], "Focus": [17741.3], "XYStage": [ 21059, 31508 ] }, "GridColumnIndex": 0 }, { "Label": "Pos9", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.82], "Focus": [17741.3], "XYStage": [ 19731.9, 31506.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos10", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.79], "Focus": [17741.4], "XYStage": [ 14528.5, 32007.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos11", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.83], "Focus": [17741.2], "XYStage": [ 13830.300000000001, 32009.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos12", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.79], "Focus": [17741.3], "XYStage": [ 8775.300000000001, 32017.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos13", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.815], "Focus": [17741.3], "XYStage": [ 8109.200000000001, 32008.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos14", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.805], "Focus": [17741.3], "XYStage": [ 3483.3, 31908.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos15", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.82], "Focus": [17741.2], "XYStage": [ 2686.8, 31903.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos16", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [211.48000000000002], "Focus": [17741.1], "XYStage": [ -1978.9, 31973.600000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos17", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [211.51500000000001], "Focus": [17741.1], "XYStage": [ -2957.8, 31982.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos18", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.195], "Focus": [17741.5], "XYStage": [ 47747.4, -576.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos19", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.23000000000002], "Focus": [17741.6], "XYStage": [ 47053.9, -576.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos20", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.205], "Focus": [17741.4], "XYStage": [ 41871.200000000004, -479.8 ] }, "GridColumnIndex": 0 }, { "Label": "Pos21", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.4], "XYStage": [ 40882.600000000006, -392 ] }, "GridColumnIndex": 0 }, { "Label": "Pos22", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.24], "Focus": [17741.4], "XYStage": [ 35905.6, -135.3 ] }, "GridColumnIndex": 0 }, { "Label": "Pos23", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.195], "Focus": [17741.5], "XYStage": [ 34990.9, -132.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos24", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.46], "Focus": [17741.4], "XYStage": [ 29678.100000000002, -958.1 ] }, "GridColumnIndex": 0 }, { "Label": "Pos25", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.44], "Focus": [17741.5], "XYStage": [ 28983.600000000002, -939.4000000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos26", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.475], "Focus": [17741.5], "XYStage": [ 23019.7, -122 ] }, "GridColumnIndex": 0 }, { "Label": "Pos27", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.44], "Focus": [17741.7], "XYStage": [ 22428, -116.80000000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos28", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.46], "Focus": [17741.7], "XYStage": [ 16364.900000000001, -181.10000000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos29", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.48000000000002], "Focus": [17741.5], "XYStage": [ 15712.1, -180 ] }, "GridColumnIndex": 0 }, { "Label": "Pos30", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.45000000000002], "Focus": [17741.7], "XYStage": [ 10346.1, 77.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos31", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.49], "Focus": [17741.4], "XYStage": [ 9755.5, 85.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos32", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.475], "Focus": [17741.5], "XYStage": [ 4775.5, 293.90000000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos33", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.445], "Focus": [17741.4], "XYStage": [ 3954.9, 293.90000000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos34", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.455], "Focus": [17741.4], "XYStage": [ -1324.9, 545.6 ] }, "GridColumnIndex": 0 }, { "Label": "Pos35", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.445], "Focus": [17741.3], "XYStage": [ -2097, 576.6 ] }, "GridColumnIndex": 0 } ], "z-step_um": 10, "GridRow": 0, "BitDepth": 16, "Depth": 2, "Channels": 6, "Source": "Micro-Manager", "ChColors": [ -16711681, -256, -65281, -1, -1, -16776961 ], "MetadataVersion": 10, "KeepShutterOpenChannels": true, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536, 65536 ], "CustomIntervals_ms": [], "Height": 1200, "MicroManagerVersion": "1.4.23 20180219", "GridColumn": 0, "UUID": "18808e7c-f2e6-47b3-90d8-1d779ce87dbd", "PixelType": "GRAY16", "Width": 1200, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\Yan\\20191218-YC113-S7S8-miseq", "Frames": 1, "Comment": "", "UserName": "Nikon", "PixelSize_um": 1.1, "ROI": [ 0, 0, 1200, 1200 ], "Date": "2019-12-19", "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC", "P-DAPI-high" ], "KeepShutterOpenSlices": true, "IJType": 1, "ComputerName": "ZadorConfocal2", "Positions": 36, "PositionIndex": 34, "CameraTimeout": 20000, "TimeFirst": false }, "640 AO-Name": "AnalogIO", "Vincent-D3-Command": "Close" }, "FrameKey-0-5-2": { "COM4-StopBits": "1", "528 AO-MaxVolts": "5.0000", "XLIGHT Excitation Wheel-State": "1", "470 Shutter-Name": "AnalogIO", "Core-Shutter": "405", "555 AO-Demo": "No", "XLIGHT Dichroic-State": "2", "XYStage-StepSize-Y": "0.1000", "ZPositionUm": 188.455, "XYStage-StepSize-X": "0.1000", "PRIME-FirmwareVersion": "20.28", "PRIME-ClearCycles": "2", "520-Name": "DA Shutter", "405 Shutter-Name": "AnalogIO", "PIEZO-StepSize": "0.0050", "PRIME-ClearMode": "Pre-Exposure", "470 AO-MinVolts": "0.0000", "405 AO-SupportsTriggering": "1", "CameraChannelIndex": 0, "XYStage-StartSpeed": "500.0000", "COM9-StopBits": "2", "405 AO-Description": "NI DAC", "COM4-DelayBetweenCharsMs": "0.0000", "445 AO-Block voltage": "false", "520 AO-TriggerInputLine": "/Dev1/PFI0", "555 AO-Description": "NI DAC", "520 AO-TriggerSampleRate": "100000", "COM4-Verbose": "1", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM4-Name": "COM4", "405 Shutter-Volts": "5.0000", "Vincent-D3-Port": "COM1", "PRIME-PP 4 MIN ADU AFFECTED": "200", "470 Shutter-MinVolts": "0.0000", "PRIME-PP 3 ENABLED": "Yes", "470 AO-Description": "NI DAC", "PRIME-Description": "PVCAM API device adapter", "520 Shutter-MaxVolts": "5.0000", "520 Shutter-Volts": "0.0000", "528 AO-Sequenceable": "1", "PRIME-Binning": "1x1", "555 Shutter-MaxVolts": "5.0000", "528 Shutter-TriggerSequenceLength": "1000", "405 Shutter-Block voltage": "false", "XLIGHT Spin motor-State": "1", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "Vincent-D3-Time to open (ms)": "35.0000", "Vincent-D3-Time to close (ms)": "35.0000", "445 AO-TriggerSampleRate": "100000", "640 Shutter-IOChannel": "Dev1/ao14", "555 AO-Sequenceable": "1", "520 AO-Name": "AnalogIO", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "470 AO-Block voltage": "false", "COM1-Verbose": "1", "COM9-AnswerTimeout": "10000.0000", "528 Shutter-Name": "AnalogIO", "PRIME-Trigger Timeout (secs)": "10", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "NextFrame": 0, "470 AO-TriggerSequenceLength": "1000", "PRIME-TransposeXY": "0", "445 AO-TriggerSequenceLength": "1000", "COM1-Name": "COM1", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "COM9-DataBits": "8", "COM1-AnswerTimeout": "500.0000", "Camera": "", "TE2000-Version": "V2.50-V2.50-V2.10", "528 Shutter-Volts": "0.0000", "XLIGHT Hub-Name": "XLIGHT Hub", "PIEZO-Description": "Ludl stage driver adapter", "PositionName": "Pos34", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSampleRate": "100000", "PRIME-FanSpeedSetpoint": "High", "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "405 AO-TriggerSampleRate": "100000", "470 Shutter-Block voltage": "false", "470-State": "0", "COM3-Parity": "None", "405 AO-Volts": "4.5000", "PRIME-TransposeMirrorY": "0", "PRIME-ChipName": "GS144BSI", "470 AO-Volts": "0.0000", "PRIME-TransposeMirrorX": "0", "445 Shutter-SupportsTriggering": "0", "470 Shutter-SupportsTriggering": "0", "XLIGHT Hub-Description": "XLIGHT hub", "PRIME-PreampOffLimit": "10000", "470 AO-Sequenceable": "1", "528 Shutter-MaxVolts": "5.0000", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "Core-XYStage": "XYStage", "470 AO-TriggerSampleRate": "100000", "PRIME-Timing-PreTriggerDelayNs": "24672000.0000", "528-DA Device": "528 Shutter", "Focus-Description": "Nikon TE2000 focus adapter", "FilterBlock-State": "5", "520 AO-Demo": "No", "COM3-DelayBetweenCharsMs": "0.0000", "470 Shutter-Volts": "0.0000", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "PRIME-PP 2 THRESHOLD": "125", "PRIME-PP 4 THRESHOLD": "75", "XYStage-Acceleration": "4.0000", "470 Shutter-Demo": "No", "PRIME-Name": "PMPCIECam00", "640-DA Device": "640 Shutter", "405 AO-Block voltage": "false", "ElapsedTime-ms": 1424225, "PRIME-Offset": "100", "445 AO-IOChannel": "Dev1/ao1", "640 AO-MaxVolts": "5.0000", "PositionIndex": 34, "PRIME-PP 1 ENABLED": "Yes", "COM1-BaudRate": "9600", "640-State": "0", "COM3-Verbose": "1", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "LudlController-TransmissionDelay": "0", "PRIME-FullWellCapacity": "62000", "PRIME-SMARTStreamingEnabled": "No", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "640 Shutter-Description": "NI DAC", "528-Description": "DA device that is used as a shutter", "COM4-Description": "Serial port driver (boost:asio)", "405 Shutter-Description": "NI DAC", "TE2000-Name": "TE2000", "528 Shutter-Description": "NI DAC", "COM9-Description": "Serial port driver (boost:asio)", "LudlController-Name": "LudlController", "445 Shutter-TriggerSequenceLength": "1000", "PRIME-SerialNumber": "A18B203008", "445 AO-Volts": "0.0000", "PRIME-X-dimension": "1200", "PRIME-ShutterCloseDelay": "0", "XLIGHT Disk slider-State": "2", "Position": "Default", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 AO-IOChannel": "Dev1/ao5", "520 AO-IOChannel": "Dev1/ao4", "528 AO-TriggerSequenceLength": "1000", "405 Shutter-MaxVolts": "5.0000", "470-DA Device": "470 Shutter", "PRIME-FTCapable": "No", "Core-ChannelGroup": "Channels", "555 AO-SupportsTriggering": "0", "PRIME-Y-dimension": "1200", "Frame": 0, "555 Shutter-Sequenceable": "1", "PRIME-Timing-ExposureTimeNs": "499998000.0000", "Core-ImageProcessor": "", "528-State": "0", "520 AO-Block voltage": "false", "XLIGHT Excitation Wheel-Label": "QUAD", "640 Shutter-Demo": "No", "COM3-StopBits": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "520 Shutter-IOChannel": "Dev1/ao11", "Width": 1200, "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-SupportsTriggering": "0", "640 AO-TriggerSampleRate": "100000", "520-Description": "DA device that is used as a shutter", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "405 Shutter-Sequenceable": "1", "405 Shutter-SupportsTriggering": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-TriggerSampleRate": "100000", "640 Shutter-MaxVolts": "5.0000", "555 AO-MaxVolts": "5.0000", "520 AO-TriggerSequenceLength": "1000", "COM3-BaudRate": "9600", "COM9-BaudRate": "9600", "Vincent-D3-Channel #": "Ch. #1", "PRIME-PMode": "Normal", "OpticalPath-Label": "Left(100)", "BitDepth": 16, "555 AO-TriggerSequenceLength": "1000", "445 Shutter-Description": "NI DAC", "405 AO-Sequenceable": "1", "PRIME-AcquisitionMethod": "Callbacks", "555 AO-Name": "AnalogIO", "PRIME-ShutterMode": "Pre-Exposure", "COM3-Handshaking": "Off", "445 AO-SupportsTriggering": "0", "ROI": "0-0-1200-1200", "COM9-Verbose": "1", "PRIME-Color": "OFF", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "PRIME-ShutterOpenDelay": "0", "COM1-Parity": "None", "Slice": 2, "SlicePosition": -30, "COM9-DelayBetweenCharsMs": "0.0000", "555-Name": "DA Shutter", "PIEZO-LudlSingleAxisName": "B", "XLIGHT Emission Wheel-Label": "QUAD", "640 Shutter-Name": "AnalogIO", "640 AO-Sequenceable": "1", "520 Shutter-Demo": "No", "528 Shutter-Demo": "No", "470-Name": "DA Shutter", "520-DA Device": "520 Shutter", "XYStage-Description": "Ludl XY stage driver adapter", "445 Shutter-MinVolts": "0.0000", "528 AO-TriggerSampleRate": "100000", "405 AO-MinVolts": "0.0000", "405 Shutter-TriggerSequenceLength": "1000", "Height": 1200, "555 AO-Block voltage": "false", "640 Shutter-Volts": "0.0000", "PixelType": "GRAY16", "445 Shutter-Block voltage": "false", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "520 Shutter-TriggerSequenceLength": "1000", "COM9-Name": "COM9", "Core-SLM": "", "555 AO-Volts": "0.0000", "555 Shutter-Block voltage": "false", "XYStage-Speed": "1800.0000", "520 AO-MaxVolts": "5.0000", "528 Shutter-SupportsTriggering": "0", "COM1-Description": "Serial port driver (boost:asio)", "PRIME-MetadataEnabled": "No", "520 Shutter-MinVolts": "0.0000", "470 Shutter-Sequenceable": "1", "PRIME-PreampDelay": "0", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-MinVolts": "0.0000", "ChannelIndex": 5, "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Core-AutoShutter": "1", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "Exposure-ms": 300, "PRIME-PP 2 ENABLED": "Yes", "640 Shutter-TriggerSampleRate": "100000", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "470 Shutter-IOChannel": "Dev1/ao10", "405 AO-Demo": "No", "PixelSizeUm": 1.1, "470 AO-Demo": "No", "470 Shutter-MaxVolts": "5.0000", "Vincent-D3-Name": "Vincent-D3", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FileName": "img_000000000_P-DAPI-high_002.tif", "LudlController-Reset": "Operate", "PRIME-Exposure": "300.00", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "445-Name": "DA Shutter", "555 Shutter-Name": "AnalogIO", "520 Shutter-TriggerSampleRate": "100000", "445 Shutter-IOChannel": "Dev1/ao9", "PRIME-TransposeCorrection": "0", "XYStage-StepSize": "0.1000", "445-Description": "DA device that is used as a shutter", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 AO-IOChannel": "Dev1/ao7", "520 Shutter-Name": "AnalogIO", "COM3-DataBits": "8", "640 AO-Volts": "0.0000", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "555 Shutter-IOChannel": "Dev1/ao13", "WaitInterval": null, "520 Shutter-Sequenceable": "1", "YPositionUm": 545.6, "445-State": "0", "445-DA Device": "445 Shutter", "PRIME-CircularBufferAutoSize": "ON", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "555 AO-TriggerSampleRate": "100000", "445 Shutter-Volts": "0.0000", "XLIGHT Hub-Port": "COM3", "Core-AutoFocus": "", "COM4-Handshaking": "Off", "528 Shutter-MinVolts": "0.0000", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "445 Shutter-Name": "AnalogIO", "528 Shutter-Sequenceable": "1", "XYStage-TransposeMirrorX": "0", "XYStage-TransposeMirrorY": "0", "PRIME-ReadoutRate": "100MHz 16bit", "XYStage-Name": "Stage", "640 AO-SupportsTriggering": "0", "640-Name": "DA Shutter", "640 Shutter-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "640 AO-Block voltage": "false", "445 Shutter-Sequenceable": "1", "470 AO-MaxVolts": "5.0000", "COM4-BaudRate": "9600", "470-Description": "DA device that is used as a shutter", "520 Shutter-Description": "NI DAC", "PRIME-CCDTemperature": "-14.8500", "Time": "2019-12-19 09:59:54 -0500", "640 AO-TriggerSequenceLength": "1000", "470 AO-Name": "AnalogIO", "XLIGHT Disk slider-Label": "Disk Pos 40um", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "Binning": "1x1", "TE2000-ModelType": "1", "COM3-AnswerTimeout": "500.0000", "555 Shutter-SupportsTriggering": "0", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "TE2000-Port": "COM4", "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Description": "NI DAC", "COM4-AnswerTimeout": "500.0000", "COM1-DelayBetweenCharsMs": "0.0000", "FilterBlock-Label": "Confocal", "445 AO-Name": "AnalogIO", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-Timing-ReadoutTimeNs": "24672000.0000", "528 AO-Demo": "No", "520 AO-Sequenceable": "1", "555-Description": "DA device that is used as a shutter", "405-DA Device": "405 Shutter", "405 AO-TriggerSequenceLength": "1000", "640 AO-MinVolts": "0.0000", "640 Shutter-Block voltage": "false", "FrameIndex": 0, "PRIME-Port": "CMOS", "555 AO-MinVolts": "0.0000", "COM3-Description": "Serial port driver (boost:asio)", "528 AO-Description": "NI DAC", "555 AO-IOChannel": "Dev1/ao6", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "555 AO-TriggerInputLine": "/Dev1/PFI0", "PRIME-ActualInterval-ms": "390.5200", "LudlController-Port": "COM9", "445 Shutter-Demo": "No", "PRIME-Trigger-Expose Out-Mux": "1", "SliceIndex": 2, "PRIME-CCDTemperatureSetPoint": "-15.0000", "405 Shutter-MinVolts": "0.0000", "PRIME-ExposeOutMode": "First Row", "AxisPositions": null, "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Volts": "0.0000", "528-Name": "DA Shutter", "555-State": "0", "XLIGHT Dichroic-Label": "QUAD", "445 AO-Sequenceable": "1", "405-Name": "DA Shutter", "405 AO-IOChannel": "Dev1/ao0", "Core-Camera": "PRIME", "Focus-Name": "Focus", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "405 Shutter-IOChannel": "Dev1/ao8", "640 AO-Demo": "No", "640 Shutter-SupportsTriggering": "0", "445 AO-Demo": "No", "555-DA Device": "555 Shutter", "470 Shutter-Description": "NI DAC", "528 AO-Volts": "0.0000", "COM1-DataBits": "8", "Vincent-D3-Address": "x", "520 AO-SupportsTriggering": "0", "405-Description": "DA device that is used as a shutter", "640 Shutter-MinVolts": "0.0000", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "405 AO-Name": "AnalogIO", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "640 AO-TriggerInputLine": "/Dev1/PFI0", "405 AO-TriggerInputLine": "/Dev1/PFI0", "640-Description": "DA device that is used as a shutter", "445 Shutter-TriggerSampleRate": "100000", "470 Shutter-TriggerSequenceLength": "1000", "COM1-StopBits": "1", "520 Shutter-SupportsTriggering": "0", "405-State": "1", "Core-Initialize": "1", "XLIGHT Emission Wheel-State": "3", "520 AO-Description": "NI DAC", "528 AO-TriggerInputLine": "/Dev1/PFI0", "445 Shutter-MaxVolts": "5.0000", "COM9-Handshaking": "Off", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "XLIGHT Spin motor-Label": "On (spinning)", "PRIME-PP 5 ENABLED": "No", "UUID": "fc7256e9-b847-47f9-82fd-48e577c51f45", "PRIME-TriggerMode": "Internal Trigger", "COM1-Handshaking": "Off", "PRIME-Timing-PostTriggerDelayNs": "49344000.0000", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "Focus-Position": "17741.5000", "528 Shutter-Block voltage": "false", "Channel": "P-DAPI-high", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "555 Shutter-Demo": "No", "405 Shutter-Demo": "No", "528 AO-Name": "AnalogIO", "520-State": "0", "Core-Galvo": "", "405 Shutter-TriggerSampleRate": "100000", "Focus-FocusStepSize_(nm)": "100", "PRIME-PixelType": "16bit", "COM4-DataBits": "8", "470 AO-IOChannel": "Dev1/ao2", "520 AO-MinVolts": "0.0000", "COM9-Parity": "None", "PRIME-CircularBufferEnabled": "ON", "PRIME-PP 6 Reset": "No", "640 AO-Description": "NI DAC", "Source": "PRIME", "445 AO-MinVolts": "0.0000", "PIEZO-Name": "Stage", "445 AO-Description": "NI DAC", "PRIME-CircularBufferFrameCount": "46", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "520 AO-Volts": "0.0000", "528 Shutter-TriggerSampleRate": "100000", "XPositionUm": -1324.9, "OpticalPath-State": "4", "PRIME-PP 3 THRESHOLD": "80", "PRIME-PVCAM Version": "3.7.1", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Gain": "1-HDR", "640 Shutter-TriggerSequenceLength": "1000", "528 AO-SupportsTriggering": "0", "445 AO-TriggerInputLine": "/Dev1/PFI0", "OpticalPath-Name": "OpticalPath", "PRIME-PP 4 ENABLED": "Yes", "PRIME-PVCAM Adapter Version": "1.3.29", "COM3-Name": "COM3", "405 AO-MaxVolts": "5.0000", "520 Shutter-Block voltage": "false", "PRIME-Timing-ClearingTimeNs": "49344000.0000", "Core-Focus": "PIEZO", "528 AO-Block voltage": "false", "TE2000-Description": "Nikon TE2000 hub", "528 AO-MinVolts": "0.0000", "COM4-Parity": "None", "Summary": { "Slices": 11, "ChContrastMin": [ 0, 0, 0, 0, 0, 0 ], "PixelAspect": 1, "SlicesFirst": true, "Interval_ms": 0, "Time": "2019-12-19 09:59:21 -0500", "Prefix": "seq_13", "InitialPositionList": [ { "Label": "Pos0", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.21], "Focus": [17741.5], "XYStage": [ 44542.200000000004, 31636.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos1", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.22], "Focus": [17741.4], "XYStage": [ 43801.100000000006, 31637.9 ] }, "GridColumnIndex": 0 }, { "Label": "Pos2", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.5], "XYStage": [ 38561.3, 32040.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos3", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.185], "Focus": [17741.3], "XYStage": [ 37698.4, 32041.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos4", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.16], "Focus": [17741.5], "XYStage": [ 32375.4, 31758.9 ] }, "GridColumnIndex": 0 }, { "Label": "Pos5", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.17000000000002], "Focus": [17741.3], "XYStage": [ 31770.7, 31758.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos6", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.4], "XYStage": [ 26536.800000000003, 31068.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos7", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.205], "Focus": [17741.4], "XYStage": [ 25420.2, 31068.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos8", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.85], "Focus": [17741.3], "XYStage": [ 21059, 31508 ] }, "GridColumnIndex": 0 }, { "Label": "Pos9", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.82], "Focus": [17741.3], "XYStage": [ 19731.9, 31506.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos10", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.79], "Focus": [17741.4], "XYStage": [ 14528.5, 32007.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos11", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.83], "Focus": [17741.2], "XYStage": [ 13830.300000000001, 32009.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos12", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.79], "Focus": [17741.3], "XYStage": [ 8775.300000000001, 32017.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos13", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.815], "Focus": [17741.3], "XYStage": [ 8109.200000000001, 32008.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos14", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.805], "Focus": [17741.3], "XYStage": [ 3483.3, 31908.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos15", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.82], "Focus": [17741.2], "XYStage": [ 2686.8, 31903.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos16", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [211.48000000000002], "Focus": [17741.1], "XYStage": [ -1978.9, 31973.600000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos17", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [211.51500000000001], "Focus": [17741.1], "XYStage": [ -2957.8, 31982.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos18", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.195], "Focus": [17741.5], "XYStage": [ 47747.4, -576.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos19", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.23000000000002], "Focus": [17741.6], "XYStage": [ 47053.9, -576.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos20", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.205], "Focus": [17741.4], "XYStage": [ 41871.200000000004, -479.8 ] }, "GridColumnIndex": 0 }, { "Label": "Pos21", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.4], "XYStage": [ 40882.600000000006, -392 ] }, "GridColumnIndex": 0 }, { "Label": "Pos22", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.24], "Focus": [17741.4], "XYStage": [ 35905.6, -135.3 ] }, "GridColumnIndex": 0 }, { "Label": "Pos23", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.195], "Focus": [17741.5], "XYStage": [ 34990.9, -132.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos24", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.46], "Focus": [17741.4], "XYStage": [ 29678.100000000002, -958.1 ] }, "GridColumnIndex": 0 }, { "Label": "Pos25", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.44], "Focus": [17741.5], "XYStage": [ 28983.600000000002, -939.4000000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos26", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.475], "Focus": [17741.5], "XYStage": [ 23019.7, -122 ] }, "GridColumnIndex": 0 }, { "Label": "Pos27", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.44], "Focus": [17741.7], "XYStage": [ 22428, -116.80000000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos28", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.46], "Focus": [17741.7], "XYStage": [ 16364.900000000001, -181.10000000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos29", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.48000000000002], "Focus": [17741.5], "XYStage": [ 15712.1, -180 ] }, "GridColumnIndex": 0 }, { "Label": "Pos30", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.45000000000002], "Focus": [17741.7], "XYStage": [ 10346.1, 77.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos31", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.49], "Focus": [17741.4], "XYStage": [ 9755.5, 85.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos32", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.475], "Focus": [17741.5], "XYStage": [ 4775.5, 293.90000000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos33", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.445], "Focus": [17741.4], "XYStage": [ 3954.9, 293.90000000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos34", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.455], "Focus": [17741.4], "XYStage": [ -1324.9, 545.6 ] }, "GridColumnIndex": 0 }, { "Label": "Pos35", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.445], "Focus": [17741.3], "XYStage": [ -2097, 576.6 ] }, "GridColumnIndex": 0 } ], "z-step_um": 10, "GridRow": 0, "BitDepth": 16, "Depth": 2, "Channels": 6, "Source": "Micro-Manager", "ChColors": [ -16711681, -256, -65281, -1, -1, -16776961 ], "MetadataVersion": 10, "KeepShutterOpenChannels": true, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536, 65536 ], "CustomIntervals_ms": [], "Height": 1200, "MicroManagerVersion": "1.4.23 20180219", "GridColumn": 0, "UUID": "18808e7c-f2e6-47b3-90d8-1d779ce87dbd", "PixelType": "GRAY16", "Width": 1200, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\Yan\\20191218-YC113-S7S8-miseq", "Frames": 1, "Comment": "", "UserName": "Nikon", "PixelSize_um": 1.1, "ROI": [ 0, 0, 1200, 1200 ], "Date": "2019-12-19", "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC", "P-DAPI-high" ], "KeepShutterOpenSlices": true, "IJType": 1, "ComputerName": "ZadorConfocal2", "Positions": 36, "PositionIndex": 34, "CameraTimeout": 20000, "TimeFirst": false }, "640 AO-Name": "AnalogIO", "Vincent-D3-Command": "Close" }, "FrameKey-0-5-3": { "COM4-StopBits": "1", "528 AO-MaxVolts": "5.0000", "XLIGHT Excitation Wheel-State": "1", "470 Shutter-Name": "AnalogIO", "Core-Shutter": "405", "555 AO-Demo": "No", "XLIGHT Dichroic-State": "2", "XYStage-StepSize-Y": "0.1000", "ZPositionUm": 198.455, "XYStage-StepSize-X": "0.1000", "PRIME-FirmwareVersion": "20.28", "PRIME-ClearCycles": "2", "520-Name": "DA Shutter", "405 Shutter-Name": "AnalogIO", "PIEZO-StepSize": "0.0050", "PRIME-ClearMode": "Pre-Exposure", "470 AO-MinVolts": "0.0000", "405 AO-SupportsTriggering": "1", "CameraChannelIndex": 0, "XYStage-StartSpeed": "500.0000", "COM9-StopBits": "2", "405 AO-Description": "NI DAC", "COM4-DelayBetweenCharsMs": "0.0000", "445 AO-Block voltage": "false", "520 AO-TriggerInputLine": "/Dev1/PFI0", "555 AO-Description": "NI DAC", "520 AO-TriggerSampleRate": "100000", "COM4-Verbose": "1", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM4-Name": "COM4", "405 Shutter-Volts": "5.0000", "Vincent-D3-Port": "COM1", "PRIME-PP 4 MIN ADU AFFECTED": "200", "470 Shutter-MinVolts": "0.0000", "PRIME-PP 3 ENABLED": "Yes", "470 AO-Description": "NI DAC", "PRIME-Description": "PVCAM API device adapter", "520 Shutter-MaxVolts": "5.0000", "520 Shutter-Volts": "0.0000", "528 AO-Sequenceable": "1", "PRIME-Binning": "1x1", "555 Shutter-MaxVolts": "5.0000", "528 Shutter-TriggerSequenceLength": "1000", "405 Shutter-Block voltage": "false", "XLIGHT Spin motor-State": "1", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "Vincent-D3-Time to open (ms)": "35.0000", "Vincent-D3-Time to close (ms)": "35.0000", "445 AO-TriggerSampleRate": "100000", "640 Shutter-IOChannel": "Dev1/ao14", "555 AO-Sequenceable": "1", "520 AO-Name": "AnalogIO", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "470 AO-Block voltage": "false", "COM1-Verbose": "1", "COM9-AnswerTimeout": "10000.0000", "528 Shutter-Name": "AnalogIO", "PRIME-Trigger Timeout (secs)": "10", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "NextFrame": 0, "470 AO-TriggerSequenceLength": "1000", "PRIME-TransposeXY": "0", "445 AO-TriggerSequenceLength": "1000", "COM1-Name": "COM1", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "COM9-DataBits": "8", "COM1-AnswerTimeout": "500.0000", "Camera": "", "TE2000-Version": "V2.50-V2.50-V2.10", "528 Shutter-Volts": "0.0000", "XLIGHT Hub-Name": "XLIGHT Hub", "PIEZO-Description": "Ludl stage driver adapter", "PositionName": "Pos34", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSampleRate": "100000", "PRIME-FanSpeedSetpoint": "High", "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "405 AO-TriggerSampleRate": "100000", "470 Shutter-Block voltage": "false", "470-State": "0", "COM3-Parity": "None", "405 AO-Volts": "4.5000", "PRIME-TransposeMirrorY": "0", "PRIME-ChipName": "GS144BSI", "470 AO-Volts": "0.0000", "PRIME-TransposeMirrorX": "0", "445 Shutter-SupportsTriggering": "0", "470 Shutter-SupportsTriggering": "0", "XLIGHT Hub-Description": "XLIGHT hub", "PRIME-PreampOffLimit": "10000", "470 AO-Sequenceable": "1", "528 Shutter-MaxVolts": "5.0000", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "Core-XYStage": "XYStage", "470 AO-TriggerSampleRate": "100000", "PRIME-Timing-PreTriggerDelayNs": "24672000.0000", "528-DA Device": "528 Shutter", "Focus-Description": "Nikon TE2000 focus adapter", "FilterBlock-State": "5", "520 AO-Demo": "No", "COM3-DelayBetweenCharsMs": "0.0000", "470 Shutter-Volts": "0.0000", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "PRIME-PP 2 THRESHOLD": "125", "PRIME-PP 4 THRESHOLD": "75", "XYStage-Acceleration": "4.0000", "470 Shutter-Demo": "No", "PRIME-Name": "PMPCIECam00", "640-DA Device": "640 Shutter", "405 AO-Block voltage": "false", "ElapsedTime-ms": 1424816, "PRIME-Offset": "100", "445 AO-IOChannel": "Dev1/ao1", "640 AO-MaxVolts": "5.0000", "PositionIndex": 34, "PRIME-PP 1 ENABLED": "Yes", "COM1-BaudRate": "9600", "640-State": "0", "COM3-Verbose": "1", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "LudlController-TransmissionDelay": "0", "PRIME-FullWellCapacity": "62000", "PRIME-SMARTStreamingEnabled": "No", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "640 Shutter-Description": "NI DAC", "528-Description": "DA device that is used as a shutter", "COM4-Description": "Serial port driver (boost:asio)", "405 Shutter-Description": "NI DAC", "TE2000-Name": "TE2000", "528 Shutter-Description": "NI DAC", "COM9-Description": "Serial port driver (boost:asio)", "LudlController-Name": "LudlController", "445 Shutter-TriggerSequenceLength": "1000", "PRIME-SerialNumber": "A18B203008", "445 AO-Volts": "0.0000", "PRIME-X-dimension": "1200", "PRIME-ShutterCloseDelay": "0", "XLIGHT Disk slider-State": "2", "Position": "Default", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 AO-IOChannel": "Dev1/ao5", "520 AO-IOChannel": "Dev1/ao4", "528 AO-TriggerSequenceLength": "1000", "405 Shutter-MaxVolts": "5.0000", "470-DA Device": "470 Shutter", "PRIME-FTCapable": "No", "Core-ChannelGroup": "Channels", "555 AO-SupportsTriggering": "0", "PRIME-Y-dimension": "1200", "Frame": 0, "555 Shutter-Sequenceable": "1", "PRIME-Timing-ExposureTimeNs": "499998000.0000", "Core-ImageProcessor": "", "528-State": "0", "520 AO-Block voltage": "false", "XLIGHT Excitation Wheel-Label": "QUAD", "640 Shutter-Demo": "No", "COM3-StopBits": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "520 Shutter-IOChannel": "Dev1/ao11", "Width": 1200, "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-SupportsTriggering": "0", "640 AO-TriggerSampleRate": "100000", "520-Description": "DA device that is used as a shutter", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "405 Shutter-Sequenceable": "1", "405 Shutter-SupportsTriggering": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-TriggerSampleRate": "100000", "640 Shutter-MaxVolts": "5.0000", "555 AO-MaxVolts": "5.0000", "520 AO-TriggerSequenceLength": "1000", "COM3-BaudRate": "9600", "COM9-BaudRate": "9600", "Vincent-D3-Channel #": "Ch. #1", "PRIME-PMode": "Normal", "OpticalPath-Label": "Left(100)", "BitDepth": 16, "555 AO-TriggerSequenceLength": "1000", "445 Shutter-Description": "NI DAC", "405 AO-Sequenceable": "1", "PRIME-AcquisitionMethod": "Callbacks", "555 AO-Name": "AnalogIO", "PRIME-ShutterMode": "Pre-Exposure", "COM3-Handshaking": "Off", "445 AO-SupportsTriggering": "0", "ROI": "0-0-1200-1200", "COM9-Verbose": "1", "PRIME-Color": "OFF", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "PRIME-ShutterOpenDelay": "0", "COM1-Parity": "None", "Slice": 3, "SlicePosition": -20, "COM9-DelayBetweenCharsMs": "0.0000", "555-Name": "DA Shutter", "PIEZO-LudlSingleAxisName": "B", "XLIGHT Emission Wheel-Label": "QUAD", "640 Shutter-Name": "AnalogIO", "640 AO-Sequenceable": "1", "520 Shutter-Demo": "No", "528 Shutter-Demo": "No", "470-Name": "DA Shutter", "520-DA Device": "520 Shutter", "XYStage-Description": "Ludl XY stage driver adapter", "445 Shutter-MinVolts": "0.0000", "528 AO-TriggerSampleRate": "100000", "405 AO-MinVolts": "0.0000", "405 Shutter-TriggerSequenceLength": "1000", "Height": 1200, "555 AO-Block voltage": "false", "640 Shutter-Volts": "0.0000", "PixelType": "GRAY16", "445 Shutter-Block voltage": "false", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "520 Shutter-TriggerSequenceLength": "1000", "COM9-Name": "COM9", "Core-SLM": "", "555 AO-Volts": "0.0000", "555 Shutter-Block voltage": "false", "XYStage-Speed": "1800.0000", "520 AO-MaxVolts": "5.0000", "528 Shutter-SupportsTriggering": "0", "COM1-Description": "Serial port driver (boost:asio)", "PRIME-MetadataEnabled": "No", "520 Shutter-MinVolts": "0.0000", "470 Shutter-Sequenceable": "1", "PRIME-PreampDelay": "0", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-MinVolts": "0.0000", "ChannelIndex": 5, "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Core-AutoShutter": "1", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "Exposure-ms": 300, "PRIME-PP 2 ENABLED": "Yes", "640 Shutter-TriggerSampleRate": "100000", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "470 Shutter-IOChannel": "Dev1/ao10", "405 AO-Demo": "No", "PixelSizeUm": 1.1, "470 AO-Demo": "No", "470 Shutter-MaxVolts": "5.0000", "Vincent-D3-Name": "Vincent-D3", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FileName": "img_000000000_P-DAPI-high_003.tif", "LudlController-Reset": "Operate", "PRIME-Exposure": "300.00", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "445-Name": "DA Shutter", "555 Shutter-Name": "AnalogIO", "520 Shutter-TriggerSampleRate": "100000", "445 Shutter-IOChannel": "Dev1/ao9", "PRIME-TransposeCorrection": "0", "XYStage-StepSize": "0.1000", "445-Description": "DA device that is used as a shutter", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 AO-IOChannel": "Dev1/ao7", "520 Shutter-Name": "AnalogIO", "COM3-DataBits": "8", "640 AO-Volts": "0.0000", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "555 Shutter-IOChannel": "Dev1/ao13", "WaitInterval": null, "520 Shutter-Sequenceable": "1", "YPositionUm": 545.6, "445-State": "0", "445-DA Device": "445 Shutter", "PRIME-CircularBufferAutoSize": "ON", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "555 AO-TriggerSampleRate": "100000", "445 Shutter-Volts": "0.0000", "XLIGHT Hub-Port": "COM3", "Core-AutoFocus": "", "COM4-Handshaking": "Off", "528 Shutter-MinVolts": "0.0000", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "445 Shutter-Name": "AnalogIO", "528 Shutter-Sequenceable": "1", "XYStage-TransposeMirrorX": "0", "XYStage-TransposeMirrorY": "0", "PRIME-ReadoutRate": "100MHz 16bit", "XYStage-Name": "Stage", "640 AO-SupportsTriggering": "0", "640-Name": "DA Shutter", "640 Shutter-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "640 AO-Block voltage": "false", "445 Shutter-Sequenceable": "1", "470 AO-MaxVolts": "5.0000", "COM4-BaudRate": "9600", "470-Description": "DA device that is used as a shutter", "520 Shutter-Description": "NI DAC", "PRIME-CCDTemperature": "-14.8500", "Time": "2019-12-19 09:59:55 -0500", "640 AO-TriggerSequenceLength": "1000", "470 AO-Name": "AnalogIO", "XLIGHT Disk slider-Label": "Disk Pos 40um", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "Binning": "1x1", "TE2000-ModelType": "1", "COM3-AnswerTimeout": "500.0000", "555 Shutter-SupportsTriggering": "0", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "TE2000-Port": "COM4", "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Description": "NI DAC", "COM4-AnswerTimeout": "500.0000", "COM1-DelayBetweenCharsMs": "0.0000", "FilterBlock-Label": "Confocal", "445 AO-Name": "AnalogIO", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-Timing-ReadoutTimeNs": "24672000.0000", "528 AO-Demo": "No", "520 AO-Sequenceable": "1", "555-Description": "DA device that is used as a shutter", "405-DA Device": "405 Shutter", "405 AO-TriggerSequenceLength": "1000", "640 AO-MinVolts": "0.0000", "640 Shutter-Block voltage": "false", "FrameIndex": 0, "PRIME-Port": "CMOS", "555 AO-MinVolts": "0.0000", "COM3-Description": "Serial port driver (boost:asio)", "528 AO-Description": "NI DAC", "555 AO-IOChannel": "Dev1/ao6", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "555 AO-TriggerInputLine": "/Dev1/PFI0", "PRIME-ActualInterval-ms": "390.5200", "LudlController-Port": "COM9", "445 Shutter-Demo": "No", "PRIME-Trigger-Expose Out-Mux": "1", "SliceIndex": 3, "PRIME-CCDTemperatureSetPoint": "-15.0000", "405 Shutter-MinVolts": "0.0000", "PRIME-ExposeOutMode": "First Row", "AxisPositions": null, "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Volts": "0.0000", "528-Name": "DA Shutter", "555-State": "0", "XLIGHT Dichroic-Label": "QUAD", "445 AO-Sequenceable": "1", "405-Name": "DA Shutter", "405 AO-IOChannel": "Dev1/ao0", "Core-Camera": "PRIME", "Focus-Name": "Focus", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "405 Shutter-IOChannel": "Dev1/ao8", "640 AO-Demo": "No", "640 Shutter-SupportsTriggering": "0", "445 AO-Demo": "No", "555-DA Device": "555 Shutter", "470 Shutter-Description": "NI DAC", "528 AO-Volts": "0.0000", "COM1-DataBits": "8", "Vincent-D3-Address": "x", "520 AO-SupportsTriggering": "0", "405-Description": "DA device that is used as a shutter", "640 Shutter-MinVolts": "0.0000", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "405 AO-Name": "AnalogIO", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "640 AO-TriggerInputLine": "/Dev1/PFI0", "405 AO-TriggerInputLine": "/Dev1/PFI0", "640-Description": "DA device that is used as a shutter", "445 Shutter-TriggerSampleRate": "100000", "470 Shutter-TriggerSequenceLength": "1000", "COM1-StopBits": "1", "520 Shutter-SupportsTriggering": "0", "405-State": "1", "Core-Initialize": "1", "XLIGHT Emission Wheel-State": "3", "520 AO-Description": "NI DAC", "528 AO-TriggerInputLine": "/Dev1/PFI0", "445 Shutter-MaxVolts": "5.0000", "COM9-Handshaking": "Off", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "XLIGHT Spin motor-Label": "On (spinning)", "PRIME-PP 5 ENABLED": "No", "UUID": "207055dc-55f3-4e8e-b19e-9662e6ffc72a", "PRIME-TriggerMode": "Internal Trigger", "COM1-Handshaking": "Off", "PRIME-Timing-PostTriggerDelayNs": "49344000.0000", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "Focus-Position": "17741.5000", "528 Shutter-Block voltage": "false", "Channel": "P-DAPI-high", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "555 Shutter-Demo": "No", "405 Shutter-Demo": "No", "528 AO-Name": "AnalogIO", "520-State": "0", "Core-Galvo": "", "405 Shutter-TriggerSampleRate": "100000", "Focus-FocusStepSize_(nm)": "100", "PRIME-PixelType": "16bit", "COM4-DataBits": "8", "470 AO-IOChannel": "Dev1/ao2", "520 AO-MinVolts": "0.0000", "COM9-Parity": "None", "PRIME-CircularBufferEnabled": "ON", "PRIME-PP 6 Reset": "No", "640 AO-Description": "NI DAC", "Source": "PRIME", "445 AO-MinVolts": "0.0000", "PIEZO-Name": "Stage", "445 AO-Description": "NI DAC", "PRIME-CircularBufferFrameCount": "46", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "520 AO-Volts": "0.0000", "528 Shutter-TriggerSampleRate": "100000", "XPositionUm": -1324.9, "OpticalPath-State": "4", "PRIME-PP 3 THRESHOLD": "80", "PRIME-PVCAM Version": "3.7.1", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Gain": "1-HDR", "640 Shutter-TriggerSequenceLength": "1000", "528 AO-SupportsTriggering": "0", "445 AO-TriggerInputLine": "/Dev1/PFI0", "OpticalPath-Name": "OpticalPath", "PRIME-PP 4 ENABLED": "Yes", "PRIME-PVCAM Adapter Version": "1.3.29", "COM3-Name": "COM3", "405 AO-MaxVolts": "5.0000", "520 Shutter-Block voltage": "false", "PRIME-Timing-ClearingTimeNs": "49344000.0000", "Core-Focus": "PIEZO", "528 AO-Block voltage": "false", "TE2000-Description": "Nikon TE2000 hub", "528 AO-MinVolts": "0.0000", "COM4-Parity": "None", "Summary": { "Slices": 11, "ChContrastMin": [ 0, 0, 0, 0, 0, 0 ], "PixelAspect": 1, "SlicesFirst": true, "Interval_ms": 0, "Time": "2019-12-19 09:59:21 -0500", "Prefix": "seq_13", "InitialPositionList": [ { "Label": "Pos0", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.21], "Focus": [17741.5], "XYStage": [ 44542.200000000004, 31636.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos1", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.22], "Focus": [17741.4], "XYStage": [ 43801.100000000006, 31637.9 ] }, "GridColumnIndex": 0 }, { "Label": "Pos2", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.5], "XYStage": [ 38561.3, 32040.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos3", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.185], "Focus": [17741.3], "XYStage": [ 37698.4, 32041.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos4", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.16], "Focus": [17741.5], "XYStage": [ 32375.4, 31758.9 ] }, "GridColumnIndex": 0 }, { "Label": "Pos5", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.17000000000002], "Focus": [17741.3], "XYStage": [ 31770.7, 31758.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos6", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.4], "XYStage": [ 26536.800000000003, 31068.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos7", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.205], "Focus": [17741.4], "XYStage": [ 25420.2, 31068.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos8", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.85], "Focus": [17741.3], "XYStage": [ 21059, 31508 ] }, "GridColumnIndex": 0 }, { "Label": "Pos9", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.82], "Focus": [17741.3], "XYStage": [ 19731.9, 31506.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos10", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.79], "Focus": [17741.4], "XYStage": [ 14528.5, 32007.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos11", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.83], "Focus": [17741.2], "XYStage": [ 13830.300000000001, 32009.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos12", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.79], "Focus": [17741.3], "XYStage": [ 8775.300000000001, 32017.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos13", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.815], "Focus": [17741.3], "XYStage": [ 8109.200000000001, 32008.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos14", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.805], "Focus": [17741.3], "XYStage": [ 3483.3, 31908.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos15", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.82], "Focus": [17741.2], "XYStage": [ 2686.8, 31903.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos16", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [211.48000000000002], "Focus": [17741.1], "XYStage": [ -1978.9, 31973.600000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos17", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [211.51500000000001], "Focus": [17741.1], "XYStage": [ -2957.8, 31982.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos18", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.195], "Focus": [17741.5], "XYStage": [ 47747.4, -576.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos19", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.23000000000002], "Focus": [17741.6], "XYStage": [ 47053.9, -576.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos20", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.205], "Focus": [17741.4], "XYStage": [ 41871.200000000004, -479.8 ] }, "GridColumnIndex": 0 }, { "Label": "Pos21", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.4], "XYStage": [ 40882.600000000006, -392 ] }, "GridColumnIndex": 0 }, { "Label": "Pos22", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.24], "Focus": [17741.4], "XYStage": [ 35905.6, -135.3 ] }, "GridColumnIndex": 0 }, { "Label": "Pos23", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.195], "Focus": [17741.5], "XYStage": [ 34990.9, -132.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos24", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.46], "Focus": [17741.4], "XYStage": [ 29678.100000000002, -958.1 ] }, "GridColumnIndex": 0 }, { "Label": "Pos25", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.44], "Focus": [17741.5], "XYStage": [ 28983.600000000002, -939.4000000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos26", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.475], "Focus": [17741.5], "XYStage": [ 23019.7, -122 ] }, "GridColumnIndex": 0 }, { "Label": "Pos27", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.44], "Focus": [17741.7], "XYStage": [ 22428, -116.80000000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos28", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.46], "Focus": [17741.7], "XYStage": [ 16364.900000000001, -181.10000000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos29", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.48000000000002], "Focus": [17741.5], "XYStage": [ 15712.1, -180 ] }, "GridColumnIndex": 0 }, { "Label": "Pos30", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.45000000000002], "Focus": [17741.7], "XYStage": [ 10346.1, 77.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos31", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.49], "Focus": [17741.4], "XYStage": [ 9755.5, 85.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos32", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.475], "Focus": [17741.5], "XYStage": [ 4775.5, 293.90000000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos33", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.445], "Focus": [17741.4], "XYStage": [ 3954.9, 293.90000000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos34", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.455], "Focus": [17741.4], "XYStage": [ -1324.9, 545.6 ] }, "GridColumnIndex": 0 }, { "Label": "Pos35", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.445], "Focus": [17741.3], "XYStage": [ -2097, 576.6 ] }, "GridColumnIndex": 0 } ], "z-step_um": 10, "GridRow": 0, "BitDepth": 16, "Depth": 2, "Channels": 6, "Source": "Micro-Manager", "ChColors": [ -16711681, -256, -65281, -1, -1, -16776961 ], "MetadataVersion": 10, "KeepShutterOpenChannels": true, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536, 65536 ], "CustomIntervals_ms": [], "Height": 1200, "MicroManagerVersion": "1.4.23 20180219", "GridColumn": 0, "UUID": "18808e7c-f2e6-47b3-90d8-1d779ce87dbd", "PixelType": "GRAY16", "Width": 1200, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\Yan\\20191218-YC113-S7S8-miseq", "Frames": 1, "Comment": "", "UserName": "Nikon", "PixelSize_um": 1.1, "ROI": [ 0, 0, 1200, 1200 ], "Date": "2019-12-19", "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC", "P-DAPI-high" ], "KeepShutterOpenSlices": true, "IJType": 1, "ComputerName": "ZadorConfocal2", "Positions": 36, "PositionIndex": 34, "CameraTimeout": 20000, "TimeFirst": false }, "640 AO-Name": "AnalogIO", "Vincent-D3-Command": "Close" }, "FrameKey-0-5-4": { "COM4-StopBits": "1", "528 AO-MaxVolts": "5.0000", "XLIGHT Excitation Wheel-State": "1", "470 Shutter-Name": "AnalogIO", "Core-Shutter": "405", "555 AO-Demo": "No", "XLIGHT Dichroic-State": "2", "XYStage-StepSize-Y": "0.1000", "ZPositionUm": 208.455, "XYStage-StepSize-X": "0.1000", "PRIME-FirmwareVersion": "20.28", "PRIME-ClearCycles": "2", "520-Name": "DA Shutter", "405 Shutter-Name": "AnalogIO", "PIEZO-StepSize": "0.0050", "PRIME-ClearMode": "Pre-Exposure", "470 AO-MinVolts": "0.0000", "405 AO-SupportsTriggering": "1", "CameraChannelIndex": 0, "XYStage-StartSpeed": "500.0000", "COM9-StopBits": "2", "405 AO-Description": "NI DAC", "COM4-DelayBetweenCharsMs": "0.0000", "445 AO-Block voltage": "false", "520 AO-TriggerInputLine": "/Dev1/PFI0", "555 AO-Description": "NI DAC", "520 AO-TriggerSampleRate": "100000", "COM4-Verbose": "1", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM4-Name": "COM4", "405 Shutter-Volts": "5.0000", "Vincent-D3-Port": "COM1", "PRIME-PP 4 MIN ADU AFFECTED": "200", "470 Shutter-MinVolts": "0.0000", "PRIME-PP 3 ENABLED": "Yes", "470 AO-Description": "NI DAC", "PRIME-Description": "PVCAM API device adapter", "520 Shutter-MaxVolts": "5.0000", "520 Shutter-Volts": "0.0000", "528 AO-Sequenceable": "1", "PRIME-Binning": "1x1", "555 Shutter-MaxVolts": "5.0000", "528 Shutter-TriggerSequenceLength": "1000", "405 Shutter-Block voltage": "false", "XLIGHT Spin motor-State": "1", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "Vincent-D3-Time to open (ms)": "35.0000", "Vincent-D3-Time to close (ms)": "35.0000", "445 AO-TriggerSampleRate": "100000", "640 Shutter-IOChannel": "Dev1/ao14", "555 AO-Sequenceable": "1", "520 AO-Name": "AnalogIO", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "470 AO-Block voltage": "false", "COM1-Verbose": "1", "COM9-AnswerTimeout": "10000.0000", "528 Shutter-Name": "AnalogIO", "PRIME-Trigger Timeout (secs)": "10", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "NextFrame": 0, "470 AO-TriggerSequenceLength": "1000", "PRIME-TransposeXY": "0", "445 AO-TriggerSequenceLength": "1000", "COM1-Name": "COM1", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "COM9-DataBits": "8", "COM1-AnswerTimeout": "500.0000", "Camera": "", "TE2000-Version": "V2.50-V2.50-V2.10", "528 Shutter-Volts": "0.0000", "XLIGHT Hub-Name": "XLIGHT Hub", "PIEZO-Description": "Ludl stage driver adapter", "PositionName": "Pos34", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSampleRate": "100000", "PRIME-FanSpeedSetpoint": "High", "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "405 AO-TriggerSampleRate": "100000", "470 Shutter-Block voltage": "false", "470-State": "0", "COM3-Parity": "None", "405 AO-Volts": "4.5000", "PRIME-TransposeMirrorY": "0", "PRIME-ChipName": "GS144BSI", "470 AO-Volts": "0.0000", "PRIME-TransposeMirrorX": "0", "445 Shutter-SupportsTriggering": "0", "470 Shutter-SupportsTriggering": "0", "XLIGHT Hub-Description": "XLIGHT hub", "PRIME-PreampOffLimit": "10000", "470 AO-Sequenceable": "1", "528 Shutter-MaxVolts": "5.0000", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "Core-XYStage": "XYStage", "470 AO-TriggerSampleRate": "100000", "PRIME-Timing-PreTriggerDelayNs": "24672000.0000", "528-DA Device": "528 Shutter", "Focus-Description": "Nikon TE2000 focus adapter", "FilterBlock-State": "5", "520 AO-Demo": "No", "COM3-DelayBetweenCharsMs": "0.0000", "470 Shutter-Volts": "0.0000", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "PRIME-PP 2 THRESHOLD": "125", "PRIME-PP 4 THRESHOLD": "75", "XYStage-Acceleration": "4.0000", "470 Shutter-Demo": "No", "PRIME-Name": "PMPCIECam00", "640-DA Device": "640 Shutter", "405 AO-Block voltage": "false", "ElapsedTime-ms": 1425354, "PRIME-Offset": "100", "445 AO-IOChannel": "Dev1/ao1", "640 AO-MaxVolts": "5.0000", "PositionIndex": 34, "PRIME-PP 1 ENABLED": "Yes", "COM1-BaudRate": "9600", "640-State": "0", "COM3-Verbose": "1", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "LudlController-TransmissionDelay": "0", "PRIME-FullWellCapacity": "62000", "PRIME-SMARTStreamingEnabled": "No", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "640 Shutter-Description": "NI DAC", "528-Description": "DA device that is used as a shutter", "COM4-Description": "Serial port driver (boost:asio)", "405 Shutter-Description": "NI DAC", "TE2000-Name": "TE2000", "528 Shutter-Description": "NI DAC", "COM9-Description": "Serial port driver (boost:asio)", "LudlController-Name": "LudlController", "445 Shutter-TriggerSequenceLength": "1000", "PRIME-SerialNumber": "A18B203008", "445 AO-Volts": "0.0000", "PRIME-X-dimension": "1200", "PRIME-ShutterCloseDelay": "0", "XLIGHT Disk slider-State": "2", "Position": "Default", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 AO-IOChannel": "Dev1/ao5", "520 AO-IOChannel": "Dev1/ao4", "528 AO-TriggerSequenceLength": "1000", "405 Shutter-MaxVolts": "5.0000", "470-DA Device": "470 Shutter", "PRIME-FTCapable": "No", "Core-ChannelGroup": "Channels", "555 AO-SupportsTriggering": "0", "PRIME-Y-dimension": "1200", "Frame": 0, "555 Shutter-Sequenceable": "1", "PRIME-Timing-ExposureTimeNs": "499998000.0000", "Core-ImageProcessor": "", "528-State": "0", "520 AO-Block voltage": "false", "XLIGHT Excitation Wheel-Label": "QUAD", "640 Shutter-Demo": "No", "COM3-StopBits": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "520 Shutter-IOChannel": "Dev1/ao11", "Width": 1200, "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-SupportsTriggering": "0", "640 AO-TriggerSampleRate": "100000", "520-Description": "DA device that is used as a shutter", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "405 Shutter-Sequenceable": "1", "405 Shutter-SupportsTriggering": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-TriggerSampleRate": "100000", "640 Shutter-MaxVolts": "5.0000", "555 AO-MaxVolts": "5.0000", "520 AO-TriggerSequenceLength": "1000", "COM3-BaudRate": "9600", "COM9-BaudRate": "9600", "Vincent-D3-Channel #": "Ch. #1", "PRIME-PMode": "Normal", "OpticalPath-Label": "Left(100)", "BitDepth": 16, "555 AO-TriggerSequenceLength": "1000", "445 Shutter-Description": "NI DAC", "405 AO-Sequenceable": "1", "PRIME-AcquisitionMethod": "Callbacks", "555 AO-Name": "AnalogIO", "PRIME-ShutterMode": "Pre-Exposure", "COM3-Handshaking": "Off", "445 AO-SupportsTriggering": "0", "ROI": "0-0-1200-1200", "COM9-Verbose": "1", "PRIME-Color": "OFF", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "PRIME-ShutterOpenDelay": "0", "COM1-Parity": "None", "Slice": 4, "SlicePosition": -10, "COM9-DelayBetweenCharsMs": "0.0000", "555-Name": "DA Shutter", "PIEZO-LudlSingleAxisName": "B", "XLIGHT Emission Wheel-Label": "QUAD", "640 Shutter-Name": "AnalogIO", "640 AO-Sequenceable": "1", "520 Shutter-Demo": "No", "528 Shutter-Demo": "No", "470-Name": "DA Shutter", "520-DA Device": "520 Shutter", "XYStage-Description": "Ludl XY stage driver adapter", "445 Shutter-MinVolts": "0.0000", "528 AO-TriggerSampleRate": "100000", "405 AO-MinVolts": "0.0000", "405 Shutter-TriggerSequenceLength": "1000", "Height": 1200, "555 AO-Block voltage": "false", "640 Shutter-Volts": "0.0000", "PixelType": "GRAY16", "445 Shutter-Block voltage": "false", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "520 Shutter-TriggerSequenceLength": "1000", "COM9-Name": "COM9", "Core-SLM": "", "555 AO-Volts": "0.0000", "555 Shutter-Block voltage": "false", "XYStage-Speed": "1800.0000", "520 AO-MaxVolts": "5.0000", "528 Shutter-SupportsTriggering": "0", "COM1-Description": "Serial port driver (boost:asio)", "PRIME-MetadataEnabled": "No", "520 Shutter-MinVolts": "0.0000", "470 Shutter-Sequenceable": "1", "PRIME-PreampDelay": "0", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-MinVolts": "0.0000", "ChannelIndex": 5, "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Core-AutoShutter": "1", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "Exposure-ms": 300, "PRIME-PP 2 ENABLED": "Yes", "640 Shutter-TriggerSampleRate": "100000", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "470 Shutter-IOChannel": "Dev1/ao10", "405 AO-Demo": "No", "PixelSizeUm": 1.1, "470 AO-Demo": "No", "470 Shutter-MaxVolts": "5.0000", "Vincent-D3-Name": "Vincent-D3", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FileName": "img_000000000_P-DAPI-high_004.tif", "LudlController-Reset": "Operate", "PRIME-Exposure": "300.00", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "445-Name": "DA Shutter", "555 Shutter-Name": "AnalogIO", "520 Shutter-TriggerSampleRate": "100000", "445 Shutter-IOChannel": "Dev1/ao9", "PRIME-TransposeCorrection": "0", "XYStage-StepSize": "0.1000", "445-Description": "DA device that is used as a shutter", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 AO-IOChannel": "Dev1/ao7", "520 Shutter-Name": "AnalogIO", "COM3-DataBits": "8", "640 AO-Volts": "0.0000", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "555 Shutter-IOChannel": "Dev1/ao13", "WaitInterval": null, "520 Shutter-Sequenceable": "1", "YPositionUm": 545.6, "445-State": "0", "445-DA Device": "445 Shutter", "PRIME-CircularBufferAutoSize": "ON", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "555 AO-TriggerSampleRate": "100000", "445 Shutter-Volts": "0.0000", "XLIGHT Hub-Port": "COM3", "Core-AutoFocus": "", "COM4-Handshaking": "Off", "528 Shutter-MinVolts": "0.0000", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "445 Shutter-Name": "AnalogIO", "528 Shutter-Sequenceable": "1", "XYStage-TransposeMirrorX": "0", "XYStage-TransposeMirrorY": "0", "PRIME-ReadoutRate": "100MHz 16bit", "XYStage-Name": "Stage", "640 AO-SupportsTriggering": "0", "640-Name": "DA Shutter", "640 Shutter-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "640 AO-Block voltage": "false", "445 Shutter-Sequenceable": "1", "470 AO-MaxVolts": "5.0000", "COM4-BaudRate": "9600", "470-Description": "DA device that is used as a shutter", "520 Shutter-Description": "NI DAC", "PRIME-CCDTemperature": "-14.8500", "Time": "2019-12-19 09:59:55 -0500", "640 AO-TriggerSequenceLength": "1000", "470 AO-Name": "AnalogIO", "XLIGHT Disk slider-Label": "Disk Pos 40um", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "Binning": "1x1", "TE2000-ModelType": "1", "COM3-AnswerTimeout": "500.0000", "555 Shutter-SupportsTriggering": "0", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "TE2000-Port": "COM4", "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Description": "NI DAC", "COM4-AnswerTimeout": "500.0000", "COM1-DelayBetweenCharsMs": "0.0000", "FilterBlock-Label": "Confocal", "445 AO-Name": "AnalogIO", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-Timing-ReadoutTimeNs": "24672000.0000", "528 AO-Demo": "No", "520 AO-Sequenceable": "1", "555-Description": "DA device that is used as a shutter", "405-DA Device": "405 Shutter", "405 AO-TriggerSequenceLength": "1000", "640 AO-MinVolts": "0.0000", "640 Shutter-Block voltage": "false", "FrameIndex": 0, "PRIME-Port": "CMOS", "555 AO-MinVolts": "0.0000", "COM3-Description": "Serial port driver (boost:asio)", "528 AO-Description": "NI DAC", "555 AO-IOChannel": "Dev1/ao6", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "555 AO-TriggerInputLine": "/Dev1/PFI0", "PRIME-ActualInterval-ms": "390.5200", "LudlController-Port": "COM9", "445 Shutter-Demo": "No", "PRIME-Trigger-Expose Out-Mux": "1", "SliceIndex": 4, "PRIME-CCDTemperatureSetPoint": "-15.0000", "405 Shutter-MinVolts": "0.0000", "PRIME-ExposeOutMode": "First Row", "AxisPositions": null, "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Volts": "0.0000", "528-Name": "DA Shutter", "555-State": "0", "XLIGHT Dichroic-Label": "QUAD", "445 AO-Sequenceable": "1", "405-Name": "DA Shutter", "405 AO-IOChannel": "Dev1/ao0", "Core-Camera": "PRIME", "Focus-Name": "Focus", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "405 Shutter-IOChannel": "Dev1/ao8", "640 AO-Demo": "No", "640 Shutter-SupportsTriggering": "0", "445 AO-Demo": "No", "555-DA Device": "555 Shutter", "470 Shutter-Description": "NI DAC", "528 AO-Volts": "0.0000", "COM1-DataBits": "8", "Vincent-D3-Address": "x", "520 AO-SupportsTriggering": "0", "405-Description": "DA device that is used as a shutter", "640 Shutter-MinVolts": "0.0000", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "405 AO-Name": "AnalogIO", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "640 AO-TriggerInputLine": "/Dev1/PFI0", "405 AO-TriggerInputLine": "/Dev1/PFI0", "640-Description": "DA device that is used as a shutter", "445 Shutter-TriggerSampleRate": "100000", "470 Shutter-TriggerSequenceLength": "1000", "COM1-StopBits": "1", "520 Shutter-SupportsTriggering": "0", "405-State": "1", "Core-Initialize": "1", "XLIGHT Emission Wheel-State": "3", "520 AO-Description": "NI DAC", "528 AO-TriggerInputLine": "/Dev1/PFI0", "445 Shutter-MaxVolts": "5.0000", "COM9-Handshaking": "Off", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "XLIGHT Spin motor-Label": "On (spinning)", "PRIME-PP 5 ENABLED": "No", "UUID": "07d1b594-0c59-4054-8a8a-03c93247acf6", "PRIME-TriggerMode": "Internal Trigger", "COM1-Handshaking": "Off", "PRIME-Timing-PostTriggerDelayNs": "49344000.0000", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "Focus-Position": "17741.5000", "528 Shutter-Block voltage": "false", "Channel": "P-DAPI-high", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "555 Shutter-Demo": "No", "405 Shutter-Demo": "No", "528 AO-Name": "AnalogIO", "520-State": "0", "Core-Galvo": "", "405 Shutter-TriggerSampleRate": "100000", "Focus-FocusStepSize_(nm)": "100", "PRIME-PixelType": "16bit", "COM4-DataBits": "8", "470 AO-IOChannel": "Dev1/ao2", "520 AO-MinVolts": "0.0000", "COM9-Parity": "None", "PRIME-CircularBufferEnabled": "ON", "PRIME-PP 6 Reset": "No", "640 AO-Description": "NI DAC", "Source": "PRIME", "445 AO-MinVolts": "0.0000", "PIEZO-Name": "Stage", "445 AO-Description": "NI DAC", "PRIME-CircularBufferFrameCount": "46", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "520 AO-Volts": "0.0000", "528 Shutter-TriggerSampleRate": "100000", "XPositionUm": -1324.9, "OpticalPath-State": "4", "PRIME-PP 3 THRESHOLD": "80", "PRIME-PVCAM Version": "3.7.1", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Gain": "1-HDR", "640 Shutter-TriggerSequenceLength": "1000", "528 AO-SupportsTriggering": "0", "445 AO-TriggerInputLine": "/Dev1/PFI0", "OpticalPath-Name": "OpticalPath", "PRIME-PP 4 ENABLED": "Yes", "PRIME-PVCAM Adapter Version": "1.3.29", "COM3-Name": "COM3", "405 AO-MaxVolts": "5.0000", "520 Shutter-Block voltage": "false", "PRIME-Timing-ClearingTimeNs": "49344000.0000", "Core-Focus": "PIEZO", "528 AO-Block voltage": "false", "TE2000-Description": "Nikon TE2000 hub", "528 AO-MinVolts": "0.0000", "COM4-Parity": "None", "Summary": { "Slices": 11, "ChContrastMin": [ 0, 0, 0, 0, 0, 0 ], "PixelAspect": 1, "SlicesFirst": true, "Interval_ms": 0, "Time": "2019-12-19 09:59:21 -0500", "Prefix": "seq_13", "InitialPositionList": [ { "Label": "Pos0", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.21], "Focus": [17741.5], "XYStage": [ 44542.200000000004, 31636.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos1", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.22], "Focus": [17741.4], "XYStage": [ 43801.100000000006, 31637.9 ] }, "GridColumnIndex": 0 }, { "Label": "Pos2", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.5], "XYStage": [ 38561.3, 32040.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos3", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.185], "Focus": [17741.3], "XYStage": [ 37698.4, 32041.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos4", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.16], "Focus": [17741.5], "XYStage": [ 32375.4, 31758.9 ] }, "GridColumnIndex": 0 }, { "Label": "Pos5", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.17000000000002], "Focus": [17741.3], "XYStage": [ 31770.7, 31758.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos6", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.4], "XYStage": [ 26536.800000000003, 31068.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos7", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.205], "Focus": [17741.4], "XYStage": [ 25420.2, 31068.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos8", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.85], "Focus": [17741.3], "XYStage": [ 21059, 31508 ] }, "GridColumnIndex": 0 }, { "Label": "Pos9", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.82], "Focus": [17741.3], "XYStage": [ 19731.9, 31506.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos10", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.79], "Focus": [17741.4], "XYStage": [ 14528.5, 32007.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos11", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.83], "Focus": [17741.2], "XYStage": [ 13830.300000000001, 32009.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos12", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.79], "Focus": [17741.3], "XYStage": [ 8775.300000000001, 32017.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos13", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.815], "Focus": [17741.3], "XYStage": [ 8109.200000000001, 32008.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos14", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.805], "Focus": [17741.3], "XYStage": [ 3483.3, 31908.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos15", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.82], "Focus": [17741.2], "XYStage": [ 2686.8, 31903.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos16", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [211.48000000000002], "Focus": [17741.1], "XYStage": [ -1978.9, 31973.600000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos17", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [211.51500000000001], "Focus": [17741.1], "XYStage": [ -2957.8, 31982.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos18", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.195], "Focus": [17741.5], "XYStage": [ 47747.4, -576.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos19", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.23000000000002], "Focus": [17741.6], "XYStage": [ 47053.9, -576.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos20", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.205], "Focus": [17741.4], "XYStage": [ 41871.200000000004, -479.8 ] }, "GridColumnIndex": 0 }, { "Label": "Pos21", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.4], "XYStage": [ 40882.600000000006, -392 ] }, "GridColumnIndex": 0 }, { "Label": "Pos22", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.24], "Focus": [17741.4], "XYStage": [ 35905.6, -135.3 ] }, "GridColumnIndex": 0 }, { "Label": "Pos23", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.195], "Focus": [17741.5], "XYStage": [ 34990.9, -132.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos24", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.46], "Focus": [17741.4], "XYStage": [ 29678.100000000002, -958.1 ] }, "GridColumnIndex": 0 }, { "Label": "Pos25", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.44], "Focus": [17741.5], "XYStage": [ 28983.600000000002, -939.4000000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos26", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.475], "Focus": [17741.5], "XYStage": [ 23019.7, -122 ] }, "GridColumnIndex": 0 }, { "Label": "Pos27", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.44], "Focus": [17741.7], "XYStage": [ 22428, -116.80000000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos28", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.46], "Focus": [17741.7], "XYStage": [ 16364.900000000001, -181.10000000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos29", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.48000000000002], "Focus": [17741.5], "XYStage": [ 15712.1, -180 ] }, "GridColumnIndex": 0 }, { "Label": "Pos30", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.45000000000002], "Focus": [17741.7], "XYStage": [ 10346.1, 77.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos31", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.49], "Focus": [17741.4], "XYStage": [ 9755.5, 85.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos32", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.475], "Focus": [17741.5], "XYStage": [ 4775.5, 293.90000000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos33", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.445], "Focus": [17741.4], "XYStage": [ 3954.9, 293.90000000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos34", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.455], "Focus": [17741.4], "XYStage": [ -1324.9, 545.6 ] }, "GridColumnIndex": 0 }, { "Label": "Pos35", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.445], "Focus": [17741.3], "XYStage": [ -2097, 576.6 ] }, "GridColumnIndex": 0 } ], "z-step_um": 10, "GridRow": 0, "BitDepth": 16, "Depth": 2, "Channels": 6, "Source": "Micro-Manager", "ChColors": [ -16711681, -256, -65281, -1, -1, -16776961 ], "MetadataVersion": 10, "KeepShutterOpenChannels": true, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536, 65536 ], "CustomIntervals_ms": [], "Height": 1200, "MicroManagerVersion": "1.4.23 20180219", "GridColumn": 0, "UUID": "18808e7c-f2e6-47b3-90d8-1d779ce87dbd", "PixelType": "GRAY16", "Width": 1200, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\Yan\\20191218-YC113-S7S8-miseq", "Frames": 1, "Comment": "", "UserName": "Nikon", "PixelSize_um": 1.1, "ROI": [ 0, 0, 1200, 1200 ], "Date": "2019-12-19", "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC", "P-DAPI-high" ], "KeepShutterOpenSlices": true, "IJType": 1, "ComputerName": "ZadorConfocal2", "Positions": 36, "PositionIndex": 34, "CameraTimeout": 20000, "TimeFirst": false }, "640 AO-Name": "AnalogIO", "Vincent-D3-Command": "Close" }, "FrameKey-0-5-5": { "COM4-StopBits": "1", "528 AO-MaxVolts": "5.0000", "XLIGHT Excitation Wheel-State": "1", "470 Shutter-Name": "AnalogIO", "Core-Shutter": "405", "555 AO-Demo": "No", "XLIGHT Dichroic-State": "2", "XYStage-StepSize-Y": "0.1000", "ZPositionUm": 218.455, "XYStage-StepSize-X": "0.1000", "PRIME-FirmwareVersion": "20.28", "PRIME-ClearCycles": "2", "520-Name": "DA Shutter", "405 Shutter-Name": "AnalogIO", "PIEZO-StepSize": "0.0050", "PRIME-ClearMode": "Pre-Exposure", "470 AO-MinVolts": "0.0000", "405 AO-SupportsTriggering": "1", "CameraChannelIndex": 0, "XYStage-StartSpeed": "500.0000", "COM9-StopBits": "2", "405 AO-Description": "NI DAC", "COM4-DelayBetweenCharsMs": "0.0000", "445 AO-Block voltage": "false", "520 AO-TriggerInputLine": "/Dev1/PFI0", "555 AO-Description": "NI DAC", "520 AO-TriggerSampleRate": "100000", "COM4-Verbose": "1", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM4-Name": "COM4", "405 Shutter-Volts": "5.0000", "Vincent-D3-Port": "COM1", "PRIME-PP 4 MIN ADU AFFECTED": "200", "470 Shutter-MinVolts": "0.0000", "PRIME-PP 3 ENABLED": "Yes", "470 AO-Description": "NI DAC", "PRIME-Description": "PVCAM API device adapter", "520 Shutter-MaxVolts": "5.0000", "520 Shutter-Volts": "0.0000", "528 AO-Sequenceable": "1", "PRIME-Binning": "1x1", "555 Shutter-MaxVolts": "5.0000", "528 Shutter-TriggerSequenceLength": "1000", "405 Shutter-Block voltage": "false", "XLIGHT Spin motor-State": "1", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "Vincent-D3-Time to open (ms)": "35.0000", "Vincent-D3-Time to close (ms)": "35.0000", "445 AO-TriggerSampleRate": "100000", "640 Shutter-IOChannel": "Dev1/ao14", "555 AO-Sequenceable": "1", "520 AO-Name": "AnalogIO", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "470 AO-Block voltage": "false", "COM1-Verbose": "1", "COM9-AnswerTimeout": "10000.0000", "528 Shutter-Name": "AnalogIO", "PRIME-Trigger Timeout (secs)": "10", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "NextFrame": 0, "470 AO-TriggerSequenceLength": "1000", "PRIME-TransposeXY": "0", "445 AO-TriggerSequenceLength": "1000", "COM1-Name": "COM1", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "COM9-DataBits": "8", "COM1-AnswerTimeout": "500.0000", "Camera": "", "TE2000-Version": "V2.50-V2.50-V2.10", "528 Shutter-Volts": "0.0000", "XLIGHT Hub-Name": "XLIGHT Hub", "PIEZO-Description": "Ludl stage driver adapter", "PositionName": "Pos34", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSampleRate": "100000", "PRIME-FanSpeedSetpoint": "High", "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "405 AO-TriggerSampleRate": "100000", "470 Shutter-Block voltage": "false", "470-State": "0", "COM3-Parity": "None", "405 AO-Volts": "4.5000", "PRIME-TransposeMirrorY": "0", "PRIME-ChipName": "GS144BSI", "470 AO-Volts": "0.0000", "PRIME-TransposeMirrorX": "0", "445 Shutter-SupportsTriggering": "0", "470 Shutter-SupportsTriggering": "0", "XLIGHT Hub-Description": "XLIGHT hub", "PRIME-PreampOffLimit": "10000", "470 AO-Sequenceable": "1", "528 Shutter-MaxVolts": "5.0000", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "Core-XYStage": "XYStage", "470 AO-TriggerSampleRate": "100000", "PRIME-Timing-PreTriggerDelayNs": "24672000.0000", "528-DA Device": "528 Shutter", "Focus-Description": "Nikon TE2000 focus adapter", "FilterBlock-State": "5", "520 AO-Demo": "No", "COM3-DelayBetweenCharsMs": "0.0000", "470 Shutter-Volts": "0.0000", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "PRIME-PP 2 THRESHOLD": "125", "PRIME-PP 4 THRESHOLD": "75", "XYStage-Acceleration": "4.0000", "470 Shutter-Demo": "No", "PRIME-Name": "PMPCIECam00", "640-DA Device": "640 Shutter", "405 AO-Block voltage": "false", "ElapsedTime-ms": 1425898, "PRIME-Offset": "100", "445 AO-IOChannel": "Dev1/ao1", "640 AO-MaxVolts": "5.0000", "PositionIndex": 34, "PRIME-PP 1 ENABLED": "Yes", "COM1-BaudRate": "9600", "640-State": "0", "COM3-Verbose": "1", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "LudlController-TransmissionDelay": "0", "PRIME-FullWellCapacity": "62000", "PRIME-SMARTStreamingEnabled": "No", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "640 Shutter-Description": "NI DAC", "528-Description": "DA device that is used as a shutter", "COM4-Description": "Serial port driver (boost:asio)", "405 Shutter-Description": "NI DAC", "TE2000-Name": "TE2000", "528 Shutter-Description": "NI DAC", "COM9-Description": "Serial port driver (boost:asio)", "LudlController-Name": "LudlController", "445 Shutter-TriggerSequenceLength": "1000", "PRIME-SerialNumber": "A18B203008", "445 AO-Volts": "0.0000", "PRIME-X-dimension": "1200", "PRIME-ShutterCloseDelay": "0", "XLIGHT Disk slider-State": "2", "Position": "Default", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 AO-IOChannel": "Dev1/ao5", "520 AO-IOChannel": "Dev1/ao4", "528 AO-TriggerSequenceLength": "1000", "405 Shutter-MaxVolts": "5.0000", "470-DA Device": "470 Shutter", "PRIME-FTCapable": "No", "Core-ChannelGroup": "Channels", "555 AO-SupportsTriggering": "0", "PRIME-Y-dimension": "1200", "Frame": 0, "555 Shutter-Sequenceable": "1", "PRIME-Timing-ExposureTimeNs": "499998000.0000", "Core-ImageProcessor": "", "528-State": "0", "520 AO-Block voltage": "false", "XLIGHT Excitation Wheel-Label": "QUAD", "640 Shutter-Demo": "No", "COM3-StopBits": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "520 Shutter-IOChannel": "Dev1/ao11", "Width": 1200, "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-SupportsTriggering": "0", "640 AO-TriggerSampleRate": "100000", "520-Description": "DA device that is used as a shutter", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "405 Shutter-Sequenceable": "1", "405 Shutter-SupportsTriggering": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-TriggerSampleRate": "100000", "640 Shutter-MaxVolts": "5.0000", "555 AO-MaxVolts": "5.0000", "520 AO-TriggerSequenceLength": "1000", "COM3-BaudRate": "9600", "COM9-BaudRate": "9600", "Vincent-D3-Channel #": "Ch. #1", "PRIME-PMode": "Normal", "OpticalPath-Label": "Left(100)", "BitDepth": 16, "555 AO-TriggerSequenceLength": "1000", "445 Shutter-Description": "NI DAC", "405 AO-Sequenceable": "1", "PRIME-AcquisitionMethod": "Callbacks", "555 AO-Name": "AnalogIO", "PRIME-ShutterMode": "Pre-Exposure", "COM3-Handshaking": "Off", "445 AO-SupportsTriggering": "0", "ROI": "0-0-1200-1200", "COM9-Verbose": "1", "PRIME-Color": "OFF", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "PRIME-ShutterOpenDelay": "0", "COM1-Parity": "None", "Slice": 5, "SlicePosition": 0, "COM9-DelayBetweenCharsMs": "0.0000", "555-Name": "DA Shutter", "PIEZO-LudlSingleAxisName": "B", "XLIGHT Emission Wheel-Label": "QUAD", "640 Shutter-Name": "AnalogIO", "640 AO-Sequenceable": "1", "520 Shutter-Demo": "No", "528 Shutter-Demo": "No", "470-Name": "DA Shutter", "520-DA Device": "520 Shutter", "XYStage-Description": "Ludl XY stage driver adapter", "445 Shutter-MinVolts": "0.0000", "528 AO-TriggerSampleRate": "100000", "405 AO-MinVolts": "0.0000", "405 Shutter-TriggerSequenceLength": "1000", "Height": 1200, "555 AO-Block voltage": "false", "640 Shutter-Volts": "0.0000", "PixelType": "GRAY16", "445 Shutter-Block voltage": "false", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "520 Shutter-TriggerSequenceLength": "1000", "COM9-Name": "COM9", "Core-SLM": "", "555 AO-Volts": "0.0000", "555 Shutter-Block voltage": "false", "XYStage-Speed": "1800.0000", "520 AO-MaxVolts": "5.0000", "528 Shutter-SupportsTriggering": "0", "COM1-Description": "Serial port driver (boost:asio)", "PRIME-MetadataEnabled": "No", "520 Shutter-MinVolts": "0.0000", "470 Shutter-Sequenceable": "1", "PRIME-PreampDelay": "0", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-MinVolts": "0.0000", "ChannelIndex": 5, "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Core-AutoShutter": "1", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "Exposure-ms": 300, "PRIME-PP 2 ENABLED": "Yes", "640 Shutter-TriggerSampleRate": "100000", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "470 Shutter-IOChannel": "Dev1/ao10", "405 AO-Demo": "No", "PixelSizeUm": 1.1, "470 AO-Demo": "No", "470 Shutter-MaxVolts": "5.0000", "Vincent-D3-Name": "Vincent-D3", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FileName": "img_000000000_P-DAPI-high_005.tif", "LudlController-Reset": "Operate", "PRIME-Exposure": "300.00", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "445-Name": "DA Shutter", "555 Shutter-Name": "AnalogIO", "520 Shutter-TriggerSampleRate": "100000", "445 Shutter-IOChannel": "Dev1/ao9", "PRIME-TransposeCorrection": "0", "XYStage-StepSize": "0.1000", "445-Description": "DA device that is used as a shutter", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 AO-IOChannel": "Dev1/ao7", "520 Shutter-Name": "AnalogIO", "COM3-DataBits": "8", "640 AO-Volts": "0.0000", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "555 Shutter-IOChannel": "Dev1/ao13", "WaitInterval": null, "520 Shutter-Sequenceable": "1", "YPositionUm": 545.6, "445-State": "0", "445-DA Device": "445 Shutter", "PRIME-CircularBufferAutoSize": "ON", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "555 AO-TriggerSampleRate": "100000", "445 Shutter-Volts": "0.0000", "XLIGHT Hub-Port": "COM3", "Core-AutoFocus": "", "COM4-Handshaking": "Off", "528 Shutter-MinVolts": "0.0000", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "445 Shutter-Name": "AnalogIO", "528 Shutter-Sequenceable": "1", "XYStage-TransposeMirrorX": "0", "XYStage-TransposeMirrorY": "0", "PRIME-ReadoutRate": "100MHz 16bit", "XYStage-Name": "Stage", "640 AO-SupportsTriggering": "0", "640-Name": "DA Shutter", "640 Shutter-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "640 AO-Block voltage": "false", "445 Shutter-Sequenceable": "1", "470 AO-MaxVolts": "5.0000", "COM4-BaudRate": "9600", "470-Description": "DA device that is used as a shutter", "520 Shutter-Description": "NI DAC", "PRIME-CCDTemperature": "-14.8500", "Time": "2019-12-19 09:59:56 -0500", "640 AO-TriggerSequenceLength": "1000", "470 AO-Name": "AnalogIO", "XLIGHT Disk slider-Label": "Disk Pos 40um", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "Binning": "1x1", "TE2000-ModelType": "1", "COM3-AnswerTimeout": "500.0000", "555 Shutter-SupportsTriggering": "0", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "TE2000-Port": "COM4", "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Description": "NI DAC", "COM4-AnswerTimeout": "500.0000", "COM1-DelayBetweenCharsMs": "0.0000", "FilterBlock-Label": "Confocal", "445 AO-Name": "AnalogIO", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-Timing-ReadoutTimeNs": "24672000.0000", "528 AO-Demo": "No", "520 AO-Sequenceable": "1", "555-Description": "DA device that is used as a shutter", "405-DA Device": "405 Shutter", "405 AO-TriggerSequenceLength": "1000", "640 AO-MinVolts": "0.0000", "640 Shutter-Block voltage": "false", "FrameIndex": 0, "PRIME-Port": "CMOS", "555 AO-MinVolts": "0.0000", "COM3-Description": "Serial port driver (boost:asio)", "528 AO-Description": "NI DAC", "555 AO-IOChannel": "Dev1/ao6", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "555 AO-TriggerInputLine": "/Dev1/PFI0", "PRIME-ActualInterval-ms": "390.5200", "LudlController-Port": "COM9", "445 Shutter-Demo": "No", "PRIME-Trigger-Expose Out-Mux": "1", "SliceIndex": 5, "PRIME-CCDTemperatureSetPoint": "-15.0000", "405 Shutter-MinVolts": "0.0000", "PRIME-ExposeOutMode": "First Row", "AxisPositions": null, "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Volts": "0.0000", "528-Name": "DA Shutter", "555-State": "0", "XLIGHT Dichroic-Label": "QUAD", "445 AO-Sequenceable": "1", "405-Name": "DA Shutter", "405 AO-IOChannel": "Dev1/ao0", "Core-Camera": "PRIME", "Focus-Name": "Focus", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "405 Shutter-IOChannel": "Dev1/ao8", "640 AO-Demo": "No", "640 Shutter-SupportsTriggering": "0", "445 AO-Demo": "No", "555-DA Device": "555 Shutter", "470 Shutter-Description": "NI DAC", "528 AO-Volts": "0.0000", "COM1-DataBits": "8", "Vincent-D3-Address": "x", "520 AO-SupportsTriggering": "0", "405-Description": "DA device that is used as a shutter", "640 Shutter-MinVolts": "0.0000", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "405 AO-Name": "AnalogIO", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "640 AO-TriggerInputLine": "/Dev1/PFI0", "405 AO-TriggerInputLine": "/Dev1/PFI0", "640-Description": "DA device that is used as a shutter", "445 Shutter-TriggerSampleRate": "100000", "470 Shutter-TriggerSequenceLength": "1000", "COM1-StopBits": "1", "520 Shutter-SupportsTriggering": "0", "405-State": "1", "Core-Initialize": "1", "XLIGHT Emission Wheel-State": "3", "520 AO-Description": "NI DAC", "528 AO-TriggerInputLine": "/Dev1/PFI0", "445 Shutter-MaxVolts": "5.0000", "COM9-Handshaking": "Off", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "XLIGHT Spin motor-Label": "On (spinning)", "PRIME-PP 5 ENABLED": "No", "UUID": "8f6daeae-6ca6-438a-8c79-38447047f23f", "PRIME-TriggerMode": "Internal Trigger", "COM1-Handshaking": "Off", "PRIME-Timing-PostTriggerDelayNs": "49344000.0000", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "Focus-Position": "17741.5000", "528 Shutter-Block voltage": "false", "Channel": "P-DAPI-high", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "555 Shutter-Demo": "No", "405 Shutter-Demo": "No", "528 AO-Name": "AnalogIO", "520-State": "0", "Core-Galvo": "", "405 Shutter-TriggerSampleRate": "100000", "Focus-FocusStepSize_(nm)": "100", "PRIME-PixelType": "16bit", "COM4-DataBits": "8", "470 AO-IOChannel": "Dev1/ao2", "520 AO-MinVolts": "0.0000", "COM9-Parity": "None", "PRIME-CircularBufferEnabled": "ON", "PRIME-PP 6 Reset": "No", "640 AO-Description": "NI DAC", "Source": "PRIME", "445 AO-MinVolts": "0.0000", "PIEZO-Name": "Stage", "445 AO-Description": "NI DAC", "PRIME-CircularBufferFrameCount": "46", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "520 AO-Volts": "0.0000", "528 Shutter-TriggerSampleRate": "100000", "XPositionUm": -1324.9, "OpticalPath-State": "4", "PRIME-PP 3 THRESHOLD": "80", "PRIME-PVCAM Version": "3.7.1", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Gain": "1-HDR", "640 Shutter-TriggerSequenceLength": "1000", "528 AO-SupportsTriggering": "0", "445 AO-TriggerInputLine": "/Dev1/PFI0", "OpticalPath-Name": "OpticalPath", "PRIME-PP 4 ENABLED": "Yes", "PRIME-PVCAM Adapter Version": "1.3.29", "COM3-Name": "COM3", "405 AO-MaxVolts": "5.0000", "520 Shutter-Block voltage": "false", "PRIME-Timing-ClearingTimeNs": "49344000.0000", "Core-Focus": "PIEZO", "528 AO-Block voltage": "false", "TE2000-Description": "Nikon TE2000 hub", "528 AO-MinVolts": "0.0000", "COM4-Parity": "None", "Summary": { "Slices": 11, "ChContrastMin": [ 0, 0, 0, 0, 0, 0 ], "PixelAspect": 1, "SlicesFirst": true, "Interval_ms": 0, "Time": "2019-12-19 09:59:21 -0500", "Prefix": "seq_13", "InitialPositionList": [ { "Label": "Pos0", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.21], "Focus": [17741.5], "XYStage": [ 44542.200000000004, 31636.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos1", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.22], "Focus": [17741.4], "XYStage": [ 43801.100000000006, 31637.9 ] }, "GridColumnIndex": 0 }, { "Label": "Pos2", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.5], "XYStage": [ 38561.3, 32040.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos3", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.185], "Focus": [17741.3], "XYStage": [ 37698.4, 32041.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos4", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.16], "Focus": [17741.5], "XYStage": [ 32375.4, 31758.9 ] }, "GridColumnIndex": 0 }, { "Label": "Pos5", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.17000000000002], "Focus": [17741.3], "XYStage": [ 31770.7, 31758.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos6", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.4], "XYStage": [ 26536.800000000003, 31068.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos7", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.205], "Focus": [17741.4], "XYStage": [ 25420.2, 31068.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos8", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.85], "Focus": [17741.3], "XYStage": [ 21059, 31508 ] }, "GridColumnIndex": 0 }, { "Label": "Pos9", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.82], "Focus": [17741.3], "XYStage": [ 19731.9, 31506.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos10", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.79], "Focus": [17741.4], "XYStage": [ 14528.5, 32007.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos11", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.83], "Focus": [17741.2], "XYStage": [ 13830.300000000001, 32009.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos12", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.79], "Focus": [17741.3], "XYStage": [ 8775.300000000001, 32017.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos13", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.815], "Focus": [17741.3], "XYStage": [ 8109.200000000001, 32008.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos14", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.805], "Focus": [17741.3], "XYStage": [ 3483.3, 31908.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos15", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.82], "Focus": [17741.2], "XYStage": [ 2686.8, 31903.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos16", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [211.48000000000002], "Focus": [17741.1], "XYStage": [ -1978.9, 31973.600000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos17", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [211.51500000000001], "Focus": [17741.1], "XYStage": [ -2957.8, 31982.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos18", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.195], "Focus": [17741.5], "XYStage": [ 47747.4, -576.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos19", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.23000000000002], "Focus": [17741.6], "XYStage": [ 47053.9, -576.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos20", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.205], "Focus": [17741.4], "XYStage": [ 41871.200000000004, -479.8 ] }, "GridColumnIndex": 0 }, { "Label": "Pos21", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.4], "XYStage": [ 40882.600000000006, -392 ] }, "GridColumnIndex": 0 }, { "Label": "Pos22", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.24], "Focus": [17741.4], "XYStage": [ 35905.6, -135.3 ] }, "GridColumnIndex": 0 }, { "Label": "Pos23", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.195], "Focus": [17741.5], "XYStage": [ 34990.9, -132.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos24", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.46], "Focus": [17741.4], "XYStage": [ 29678.100000000002, -958.1 ] }, "GridColumnIndex": 0 }, { "Label": "Pos25", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.44], "Focus": [17741.5], "XYStage": [ 28983.600000000002, -939.4000000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos26", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.475], "Focus": [17741.5], "XYStage": [ 23019.7, -122 ] }, "GridColumnIndex": 0 }, { "Label": "Pos27", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.44], "Focus": [17741.7], "XYStage": [ 22428, -116.80000000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos28", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.46], "Focus": [17741.7], "XYStage": [ 16364.900000000001, -181.10000000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos29", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.48000000000002], "Focus": [17741.5], "XYStage": [ 15712.1, -180 ] }, "GridColumnIndex": 0 }, { "Label": "Pos30", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.45000000000002], "Focus": [17741.7], "XYStage": [ 10346.1, 77.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos31", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.49], "Focus": [17741.4], "XYStage": [ 9755.5, 85.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos32", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.475], "Focus": [17741.5], "XYStage": [ 4775.5, 293.90000000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos33", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.445], "Focus": [17741.4], "XYStage": [ 3954.9, 293.90000000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos34", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.455], "Focus": [17741.4], "XYStage": [ -1324.9, 545.6 ] }, "GridColumnIndex": 0 }, { "Label": "Pos35", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.445], "Focus": [17741.3], "XYStage": [ -2097, 576.6 ] }, "GridColumnIndex": 0 } ], "z-step_um": 10, "GridRow": 0, "BitDepth": 16, "Depth": 2, "Channels": 6, "Source": "Micro-Manager", "ChColors": [ -16711681, -256, -65281, -1, -1, -16776961 ], "MetadataVersion": 10, "KeepShutterOpenChannels": true, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536, 65536 ], "CustomIntervals_ms": [], "Height": 1200, "MicroManagerVersion": "1.4.23 20180219", "GridColumn": 0, "UUID": "18808e7c-f2e6-47b3-90d8-1d779ce87dbd", "PixelType": "GRAY16", "Width": 1200, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\Yan\\20191218-YC113-S7S8-miseq", "Frames": 1, "Comment": "", "UserName": "Nikon", "PixelSize_um": 1.1, "ROI": [ 0, 0, 1200, 1200 ], "Date": "2019-12-19", "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC", "P-DAPI-high" ], "KeepShutterOpenSlices": true, "IJType": 1, "ComputerName": "ZadorConfocal2", "Positions": 36, "PositionIndex": 34, "CameraTimeout": 20000, "TimeFirst": false }, "640 AO-Name": "AnalogIO", "Vincent-D3-Command": "Close" }, "FrameKey-0-5-6": { "COM4-StopBits": "1", "528 AO-MaxVolts": "5.0000", "XLIGHT Excitation Wheel-State": "1", "470 Shutter-Name": "AnalogIO", "Core-Shutter": "405", "555 AO-Demo": "No", "XLIGHT Dichroic-State": "2", "XYStage-StepSize-Y": "0.1000", "ZPositionUm": 228.455, "XYStage-StepSize-X": "0.1000", "PRIME-FirmwareVersion": "20.28", "PRIME-ClearCycles": "2", "520-Name": "DA Shutter", "405 Shutter-Name": "AnalogIO", "PIEZO-StepSize": "0.0050", "PRIME-ClearMode": "Pre-Exposure", "470 AO-MinVolts": "0.0000", "405 AO-SupportsTriggering": "1", "CameraChannelIndex": 0, "XYStage-StartSpeed": "500.0000", "COM9-StopBits": "2", "405 AO-Description": "NI DAC", "COM4-DelayBetweenCharsMs": "0.0000", "445 AO-Block voltage": "false", "520 AO-TriggerInputLine": "/Dev1/PFI0", "555 AO-Description": "NI DAC", "520 AO-TriggerSampleRate": "100000", "COM4-Verbose": "1", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM4-Name": "COM4", "405 Shutter-Volts": "5.0000", "Vincent-D3-Port": "COM1", "PRIME-PP 4 MIN ADU AFFECTED": "200", "470 Shutter-MinVolts": "0.0000", "PRIME-PP 3 ENABLED": "Yes", "470 AO-Description": "NI DAC", "PRIME-Description": "PVCAM API device adapter", "520 Shutter-MaxVolts": "5.0000", "520 Shutter-Volts": "0.0000", "528 AO-Sequenceable": "1", "PRIME-Binning": "1x1", "555 Shutter-MaxVolts": "5.0000", "528 Shutter-TriggerSequenceLength": "1000", "405 Shutter-Block voltage": "false", "XLIGHT Spin motor-State": "1", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "Vincent-D3-Time to open (ms)": "35.0000", "Vincent-D3-Time to close (ms)": "35.0000", "445 AO-TriggerSampleRate": "100000", "640 Shutter-IOChannel": "Dev1/ao14", "555 AO-Sequenceable": "1", "520 AO-Name": "AnalogIO", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "470 AO-Block voltage": "false", "COM1-Verbose": "1", "COM9-AnswerTimeout": "10000.0000", "528 Shutter-Name": "AnalogIO", "PRIME-Trigger Timeout (secs)": "10", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "NextFrame": 0, "470 AO-TriggerSequenceLength": "1000", "PRIME-TransposeXY": "0", "445 AO-TriggerSequenceLength": "1000", "COM1-Name": "COM1", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "COM9-DataBits": "8", "COM1-AnswerTimeout": "500.0000", "Camera": "", "TE2000-Version": "V2.50-V2.50-V2.10", "528 Shutter-Volts": "0.0000", "XLIGHT Hub-Name": "XLIGHT Hub", "PIEZO-Description": "Ludl stage driver adapter", "PositionName": "Pos34", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSampleRate": "100000", "PRIME-FanSpeedSetpoint": "High", "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "405 AO-TriggerSampleRate": "100000", "470 Shutter-Block voltage": "false", "470-State": "0", "COM3-Parity": "None", "405 AO-Volts": "4.5000", "PRIME-TransposeMirrorY": "0", "PRIME-ChipName": "GS144BSI", "470 AO-Volts": "0.0000", "PRIME-TransposeMirrorX": "0", "445 Shutter-SupportsTriggering": "0", "470 Shutter-SupportsTriggering": "0", "XLIGHT Hub-Description": "XLIGHT hub", "PRIME-PreampOffLimit": "10000", "470 AO-Sequenceable": "1", "528 Shutter-MaxVolts": "5.0000", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "Core-XYStage": "XYStage", "470 AO-TriggerSampleRate": "100000", "PRIME-Timing-PreTriggerDelayNs": "24672000.0000", "528-DA Device": "528 Shutter", "Focus-Description": "Nikon TE2000 focus adapter", "FilterBlock-State": "5", "520 AO-Demo": "No", "COM3-DelayBetweenCharsMs": "0.0000", "470 Shutter-Volts": "0.0000", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "PRIME-PP 2 THRESHOLD": "125", "PRIME-PP 4 THRESHOLD": "75", "XYStage-Acceleration": "4.0000", "470 Shutter-Demo": "No", "PRIME-Name": "PMPCIECam00", "640-DA Device": "640 Shutter", "405 AO-Block voltage": "false", "ElapsedTime-ms": 1426449, "PRIME-Offset": "100", "445 AO-IOChannel": "Dev1/ao1", "640 AO-MaxVolts": "5.0000", "PositionIndex": 34, "PRIME-PP 1 ENABLED": "Yes", "COM1-BaudRate": "9600", "640-State": "0", "COM3-Verbose": "1", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "LudlController-TransmissionDelay": "0", "PRIME-FullWellCapacity": "62000", "PRIME-SMARTStreamingEnabled": "No", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "640 Shutter-Description": "NI DAC", "528-Description": "DA device that is used as a shutter", "COM4-Description": "Serial port driver (boost:asio)", "405 Shutter-Description": "NI DAC", "TE2000-Name": "TE2000", "528 Shutter-Description": "NI DAC", "COM9-Description": "Serial port driver (boost:asio)", "LudlController-Name": "LudlController", "445 Shutter-TriggerSequenceLength": "1000", "PRIME-SerialNumber": "A18B203008", "445 AO-Volts": "0.0000", "PRIME-X-dimension": "1200", "PRIME-ShutterCloseDelay": "0", "XLIGHT Disk slider-State": "2", "Position": "Default", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 AO-IOChannel": "Dev1/ao5", "520 AO-IOChannel": "Dev1/ao4", "528 AO-TriggerSequenceLength": "1000", "405 Shutter-MaxVolts": "5.0000", "470-DA Device": "470 Shutter", "PRIME-FTCapable": "No", "Core-ChannelGroup": "Channels", "555 AO-SupportsTriggering": "0", "PRIME-Y-dimension": "1200", "Frame": 0, "555 Shutter-Sequenceable": "1", "PRIME-Timing-ExposureTimeNs": "499998000.0000", "Core-ImageProcessor": "", "528-State": "0", "520 AO-Block voltage": "false", "XLIGHT Excitation Wheel-Label": "QUAD", "640 Shutter-Demo": "No", "COM3-StopBits": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "520 Shutter-IOChannel": "Dev1/ao11", "Width": 1200, "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-SupportsTriggering": "0", "640 AO-TriggerSampleRate": "100000", "520-Description": "DA device that is used as a shutter", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "405 Shutter-Sequenceable": "1", "405 Shutter-SupportsTriggering": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-TriggerSampleRate": "100000", "640 Shutter-MaxVolts": "5.0000", "555 AO-MaxVolts": "5.0000", "520 AO-TriggerSequenceLength": "1000", "COM3-BaudRate": "9600", "COM9-BaudRate": "9600", "Vincent-D3-Channel #": "Ch. #1", "PRIME-PMode": "Normal", "OpticalPath-Label": "Left(100)", "BitDepth": 16, "555 AO-TriggerSequenceLength": "1000", "445 Shutter-Description": "NI DAC", "405 AO-Sequenceable": "1", "PRIME-AcquisitionMethod": "Callbacks", "555 AO-Name": "AnalogIO", "PRIME-ShutterMode": "Pre-Exposure", "COM3-Handshaking": "Off", "445 AO-SupportsTriggering": "0", "ROI": "0-0-1200-1200", "COM9-Verbose": "1", "PRIME-Color": "OFF", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "PRIME-ShutterOpenDelay": "0", "COM1-Parity": "None", "Slice": 6, "SlicePosition": 10, "COM9-DelayBetweenCharsMs": "0.0000", "555-Name": "DA Shutter", "PIEZO-LudlSingleAxisName": "B", "XLIGHT Emission Wheel-Label": "QUAD", "640 Shutter-Name": "AnalogIO", "640 AO-Sequenceable": "1", "520 Shutter-Demo": "No", "528 Shutter-Demo": "No", "470-Name": "DA Shutter", "520-DA Device": "520 Shutter", "XYStage-Description": "Ludl XY stage driver adapter", "445 Shutter-MinVolts": "0.0000", "528 AO-TriggerSampleRate": "100000", "405 AO-MinVolts": "0.0000", "405 Shutter-TriggerSequenceLength": "1000", "Height": 1200, "555 AO-Block voltage": "false", "640 Shutter-Volts": "0.0000", "PixelType": "GRAY16", "445 Shutter-Block voltage": "false", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "520 Shutter-TriggerSequenceLength": "1000", "COM9-Name": "COM9", "Core-SLM": "", "555 AO-Volts": "0.0000", "555 Shutter-Block voltage": "false", "XYStage-Speed": "1800.0000", "520 AO-MaxVolts": "5.0000", "528 Shutter-SupportsTriggering": "0", "COM1-Description": "Serial port driver (boost:asio)", "PRIME-MetadataEnabled": "No", "520 Shutter-MinVolts": "0.0000", "470 Shutter-Sequenceable": "1", "PRIME-PreampDelay": "0", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-MinVolts": "0.0000", "ChannelIndex": 5, "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Core-AutoShutter": "1", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "Exposure-ms": 300, "PRIME-PP 2 ENABLED": "Yes", "640 Shutter-TriggerSampleRate": "100000", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "470 Shutter-IOChannel": "Dev1/ao10", "405 AO-Demo": "No", "PixelSizeUm": 1.1, "470 AO-Demo": "No", "470 Shutter-MaxVolts": "5.0000", "Vincent-D3-Name": "Vincent-D3", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FileName": "img_000000000_P-DAPI-high_006.tif", "LudlController-Reset": "Operate", "PRIME-Exposure": "300.00", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "445-Name": "DA Shutter", "555 Shutter-Name": "AnalogIO", "520 Shutter-TriggerSampleRate": "100000", "445 Shutter-IOChannel": "Dev1/ao9", "PRIME-TransposeCorrection": "0", "XYStage-StepSize": "0.1000", "445-Description": "DA device that is used as a shutter", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 AO-IOChannel": "Dev1/ao7", "520 Shutter-Name": "AnalogIO", "COM3-DataBits": "8", "640 AO-Volts": "0.0000", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "555 Shutter-IOChannel": "Dev1/ao13", "WaitInterval": null, "520 Shutter-Sequenceable": "1", "YPositionUm": 545.6, "445-State": "0", "445-DA Device": "445 Shutter", "PRIME-CircularBufferAutoSize": "ON", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "555 AO-TriggerSampleRate": "100000", "445 Shutter-Volts": "0.0000", "XLIGHT Hub-Port": "COM3", "Core-AutoFocus": "", "COM4-Handshaking": "Off", "528 Shutter-MinVolts": "0.0000", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "445 Shutter-Name": "AnalogIO", "528 Shutter-Sequenceable": "1", "XYStage-TransposeMirrorX": "0", "XYStage-TransposeMirrorY": "0", "PRIME-ReadoutRate": "100MHz 16bit", "XYStage-Name": "Stage", "640 AO-SupportsTriggering": "0", "640-Name": "DA Shutter", "640 Shutter-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "640 AO-Block voltage": "false", "445 Shutter-Sequenceable": "1", "470 AO-MaxVolts": "5.0000", "COM4-BaudRate": "9600", "470-Description": "DA device that is used as a shutter", "520 Shutter-Description": "NI DAC", "PRIME-CCDTemperature": "-14.8500", "Time": "2019-12-19 09:59:56 -0500", "640 AO-TriggerSequenceLength": "1000", "470 AO-Name": "AnalogIO", "XLIGHT Disk slider-Label": "Disk Pos 40um", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "Binning": "1x1", "TE2000-ModelType": "1", "COM3-AnswerTimeout": "500.0000", "555 Shutter-SupportsTriggering": "0", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "TE2000-Port": "COM4", "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Description": "NI DAC", "COM4-AnswerTimeout": "500.0000", "COM1-DelayBetweenCharsMs": "0.0000", "FilterBlock-Label": "Confocal", "445 AO-Name": "AnalogIO", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-Timing-ReadoutTimeNs": "24672000.0000", "528 AO-Demo": "No", "520 AO-Sequenceable": "1", "555-Description": "DA device that is used as a shutter", "405-DA Device": "405 Shutter", "405 AO-TriggerSequenceLength": "1000", "640 AO-MinVolts": "0.0000", "640 Shutter-Block voltage": "false", "FrameIndex": 0, "PRIME-Port": "CMOS", "555 AO-MinVolts": "0.0000", "COM3-Description": "Serial port driver (boost:asio)", "528 AO-Description": "NI DAC", "555 AO-IOChannel": "Dev1/ao6", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "555 AO-TriggerInputLine": "/Dev1/PFI0", "PRIME-ActualInterval-ms": "390.5200", "LudlController-Port": "COM9", "445 Shutter-Demo": "No", "PRIME-Trigger-Expose Out-Mux": "1", "SliceIndex": 6, "PRIME-CCDTemperatureSetPoint": "-15.0000", "405 Shutter-MinVolts": "0.0000", "PRIME-ExposeOutMode": "First Row", "AxisPositions": null, "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Volts": "0.0000", "528-Name": "DA Shutter", "555-State": "0", "XLIGHT Dichroic-Label": "QUAD", "445 AO-Sequenceable": "1", "405-Name": "DA Shutter", "405 AO-IOChannel": "Dev1/ao0", "Core-Camera": "PRIME", "Focus-Name": "Focus", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "405 Shutter-IOChannel": "Dev1/ao8", "640 AO-Demo": "No", "640 Shutter-SupportsTriggering": "0", "445 AO-Demo": "No", "555-DA Device": "555 Shutter", "470 Shutter-Description": "NI DAC", "528 AO-Volts": "0.0000", "COM1-DataBits": "8", "Vincent-D3-Address": "x", "520 AO-SupportsTriggering": "0", "405-Description": "DA device that is used as a shutter", "640 Shutter-MinVolts": "0.0000", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "405 AO-Name": "AnalogIO", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "640 AO-TriggerInputLine": "/Dev1/PFI0", "405 AO-TriggerInputLine": "/Dev1/PFI0", "640-Description": "DA device that is used as a shutter", "445 Shutter-TriggerSampleRate": "100000", "470 Shutter-TriggerSequenceLength": "1000", "COM1-StopBits": "1", "520 Shutter-SupportsTriggering": "0", "405-State": "1", "Core-Initialize": "1", "XLIGHT Emission Wheel-State": "3", "520 AO-Description": "NI DAC", "528 AO-TriggerInputLine": "/Dev1/PFI0", "445 Shutter-MaxVolts": "5.0000", "COM9-Handshaking": "Off", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "XLIGHT Spin motor-Label": "On (spinning)", "PRIME-PP 5 ENABLED": "No", "UUID": "6997ef27-b828-4b63-bd3c-ee24f1d598b5", "PRIME-TriggerMode": "Internal Trigger", "COM1-Handshaking": "Off", "PRIME-Timing-PostTriggerDelayNs": "49344000.0000", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "Focus-Position": "17741.5000", "528 Shutter-Block voltage": "false", "Channel": "P-DAPI-high", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "555 Shutter-Demo": "No", "405 Shutter-Demo": "No", "528 AO-Name": "AnalogIO", "520-State": "0", "Core-Galvo": "", "405 Shutter-TriggerSampleRate": "100000", "Focus-FocusStepSize_(nm)": "100", "PRIME-PixelType": "16bit", "COM4-DataBits": "8", "470 AO-IOChannel": "Dev1/ao2", "520 AO-MinVolts": "0.0000", "COM9-Parity": "None", "PRIME-CircularBufferEnabled": "ON", "PRIME-PP 6 Reset": "No", "640 AO-Description": "NI DAC", "Source": "PRIME", "445 AO-MinVolts": "0.0000", "PIEZO-Name": "Stage", "445 AO-Description": "NI DAC", "PRIME-CircularBufferFrameCount": "46", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "520 AO-Volts": "0.0000", "528 Shutter-TriggerSampleRate": "100000", "XPositionUm": -1324.9, "OpticalPath-State": "4", "PRIME-PP 3 THRESHOLD": "80", "PRIME-PVCAM Version": "3.7.1", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Gain": "1-HDR", "640 Shutter-TriggerSequenceLength": "1000", "528 AO-SupportsTriggering": "0", "445 AO-TriggerInputLine": "/Dev1/PFI0", "OpticalPath-Name": "OpticalPath", "PRIME-PP 4 ENABLED": "Yes", "PRIME-PVCAM Adapter Version": "1.3.29", "COM3-Name": "COM3", "405 AO-MaxVolts": "5.0000", "520 Shutter-Block voltage": "false", "PRIME-Timing-ClearingTimeNs": "49344000.0000", "Core-Focus": "PIEZO", "528 AO-Block voltage": "false", "TE2000-Description": "Nikon TE2000 hub", "528 AO-MinVolts": "0.0000", "COM4-Parity": "None", "Summary": { "Slices": 11, "ChContrastMin": [ 0, 0, 0, 0, 0, 0 ], "PixelAspect": 1, "SlicesFirst": true, "Interval_ms": 0, "Time": "2019-12-19 09:59:21 -0500", "Prefix": "seq_13", "InitialPositionList": [ { "Label": "Pos0", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.21], "Focus": [17741.5], "XYStage": [ 44542.200000000004, 31636.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos1", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.22], "Focus": [17741.4], "XYStage": [ 43801.100000000006, 31637.9 ] }, "GridColumnIndex": 0 }, { "Label": "Pos2", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.5], "XYStage": [ 38561.3, 32040.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos3", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.185], "Focus": [17741.3], "XYStage": [ 37698.4, 32041.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos4", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.16], "Focus": [17741.5], "XYStage": [ 32375.4, 31758.9 ] }, "GridColumnIndex": 0 }, { "Label": "Pos5", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.17000000000002], "Focus": [17741.3], "XYStage": [ 31770.7, 31758.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos6", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.4], "XYStage": [ 26536.800000000003, 31068.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos7", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.205], "Focus": [17741.4], "XYStage": [ 25420.2, 31068.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos8", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.85], "Focus": [17741.3], "XYStage": [ 21059, 31508 ] }, "GridColumnIndex": 0 }, { "Label": "Pos9", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.82], "Focus": [17741.3], "XYStage": [ 19731.9, 31506.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos10", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.79], "Focus": [17741.4], "XYStage": [ 14528.5, 32007.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos11", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.83], "Focus": [17741.2], "XYStage": [ 13830.300000000001, 32009.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos12", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.79], "Focus": [17741.3], "XYStage": [ 8775.300000000001, 32017.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos13", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.815], "Focus": [17741.3], "XYStage": [ 8109.200000000001, 32008.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos14", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.805], "Focus": [17741.3], "XYStage": [ 3483.3, 31908.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos15", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.82], "Focus": [17741.2], "XYStage": [ 2686.8, 31903.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos16", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [211.48000000000002], "Focus": [17741.1], "XYStage": [ -1978.9, 31973.600000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos17", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [211.51500000000001], "Focus": [17741.1], "XYStage": [ -2957.8, 31982.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos18", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.195], "Focus": [17741.5], "XYStage": [ 47747.4, -576.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos19", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.23000000000002], "Focus": [17741.6], "XYStage": [ 47053.9, -576.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos20", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.205], "Focus": [17741.4], "XYStage": [ 41871.200000000004, -479.8 ] }, "GridColumnIndex": 0 }, { "Label": "Pos21", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.4], "XYStage": [ 40882.600000000006, -392 ] }, "GridColumnIndex": 0 }, { "Label": "Pos22", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.24], "Focus": [17741.4], "XYStage": [ 35905.6, -135.3 ] }, "GridColumnIndex": 0 }, { "Label": "Pos23", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.195], "Focus": [17741.5], "XYStage": [ 34990.9, -132.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos24", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.46], "Focus": [17741.4], "XYStage": [ 29678.100000000002, -958.1 ] }, "GridColumnIndex": 0 }, { "Label": "Pos25", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.44], "Focus": [17741.5], "XYStage": [ 28983.600000000002, -939.4000000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos26", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.475], "Focus": [17741.5], "XYStage": [ 23019.7, -122 ] }, "GridColumnIndex": 0 }, { "Label": "Pos27", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.44], "Focus": [17741.7], "XYStage": [ 22428, -116.80000000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos28", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.46], "Focus": [17741.7], "XYStage": [ 16364.900000000001, -181.10000000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos29", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.48000000000002], "Focus": [17741.5], "XYStage": [ 15712.1, -180 ] }, "GridColumnIndex": 0 }, { "Label": "Pos30", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.45000000000002], "Focus": [17741.7], "XYStage": [ 10346.1, 77.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos31", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.49], "Focus": [17741.4], "XYStage": [ 9755.5, 85.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos32", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.475], "Focus": [17741.5], "XYStage": [ 4775.5, 293.90000000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos33", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.445], "Focus": [17741.4], "XYStage": [ 3954.9, 293.90000000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos34", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.455], "Focus": [17741.4], "XYStage": [ -1324.9, 545.6 ] }, "GridColumnIndex": 0 }, { "Label": "Pos35", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.445], "Focus": [17741.3], "XYStage": [ -2097, 576.6 ] }, "GridColumnIndex": 0 } ], "z-step_um": 10, "GridRow": 0, "BitDepth": 16, "Depth": 2, "Channels": 6, "Source": "Micro-Manager", "ChColors": [ -16711681, -256, -65281, -1, -1, -16776961 ], "MetadataVersion": 10, "KeepShutterOpenChannels": true, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536, 65536 ], "CustomIntervals_ms": [], "Height": 1200, "MicroManagerVersion": "1.4.23 20180219", "GridColumn": 0, "UUID": "18808e7c-f2e6-47b3-90d8-1d779ce87dbd", "PixelType": "GRAY16", "Width": 1200, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\Yan\\20191218-YC113-S7S8-miseq", "Frames": 1, "Comment": "", "UserName": "Nikon", "PixelSize_um": 1.1, "ROI": [ 0, 0, 1200, 1200 ], "Date": "2019-12-19", "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC", "P-DAPI-high" ], "KeepShutterOpenSlices": true, "IJType": 1, "ComputerName": "ZadorConfocal2", "Positions": 36, "PositionIndex": 34, "CameraTimeout": 20000, "TimeFirst": false }, "640 AO-Name": "AnalogIO", "Vincent-D3-Command": "Close" }, "FrameKey-0-5-7": { "COM4-StopBits": "1", "528 AO-MaxVolts": "5.0000", "XLIGHT Excitation Wheel-State": "1", "470 Shutter-Name": "AnalogIO", "Core-Shutter": "405", "555 AO-Demo": "No", "XLIGHT Dichroic-State": "2", "XYStage-StepSize-Y": "0.1000", "ZPositionUm": 238.455, "XYStage-StepSize-X": "0.1000", "PRIME-FirmwareVersion": "20.28", "PRIME-ClearCycles": "2", "520-Name": "DA Shutter", "405 Shutter-Name": "AnalogIO", "PIEZO-StepSize": "0.0050", "PRIME-ClearMode": "Pre-Exposure", "470 AO-MinVolts": "0.0000", "405 AO-SupportsTriggering": "1", "CameraChannelIndex": 0, "XYStage-StartSpeed": "500.0000", "COM9-StopBits": "2", "405 AO-Description": "NI DAC", "COM4-DelayBetweenCharsMs": "0.0000", "445 AO-Block voltage": "false", "520 AO-TriggerInputLine": "/Dev1/PFI0", "555 AO-Description": "NI DAC", "520 AO-TriggerSampleRate": "100000", "COM4-Verbose": "1", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM4-Name": "COM4", "405 Shutter-Volts": "5.0000", "Vincent-D3-Port": "COM1", "PRIME-PP 4 MIN ADU AFFECTED": "200", "470 Shutter-MinVolts": "0.0000", "PRIME-PP 3 ENABLED": "Yes", "470 AO-Description": "NI DAC", "PRIME-Description": "PVCAM API device adapter", "520 Shutter-MaxVolts": "5.0000", "520 Shutter-Volts": "0.0000", "528 AO-Sequenceable": "1", "PRIME-Binning": "1x1", "555 Shutter-MaxVolts": "5.0000", "528 Shutter-TriggerSequenceLength": "1000", "405 Shutter-Block voltage": "false", "XLIGHT Spin motor-State": "1", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "Vincent-D3-Time to open (ms)": "35.0000", "Vincent-D3-Time to close (ms)": "35.0000", "445 AO-TriggerSampleRate": "100000", "640 Shutter-IOChannel": "Dev1/ao14", "555 AO-Sequenceable": "1", "520 AO-Name": "AnalogIO", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "470 AO-Block voltage": "false", "COM1-Verbose": "1", "COM9-AnswerTimeout": "10000.0000", "528 Shutter-Name": "AnalogIO", "PRIME-Trigger Timeout (secs)": "10", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "NextFrame": 0, "470 AO-TriggerSequenceLength": "1000", "PRIME-TransposeXY": "0", "445 AO-TriggerSequenceLength": "1000", "COM1-Name": "COM1", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "COM9-DataBits": "8", "COM1-AnswerTimeout": "500.0000", "Camera": "", "TE2000-Version": "V2.50-V2.50-V2.10", "528 Shutter-Volts": "0.0000", "XLIGHT Hub-Name": "XLIGHT Hub", "PIEZO-Description": "Ludl stage driver adapter", "PositionName": "Pos34", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSampleRate": "100000", "PRIME-FanSpeedSetpoint": "High", "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "405 AO-TriggerSampleRate": "100000", "470 Shutter-Block voltage": "false", "470-State": "0", "COM3-Parity": "None", "405 AO-Volts": "4.5000", "PRIME-TransposeMirrorY": "0", "PRIME-ChipName": "GS144BSI", "470 AO-Volts": "0.0000", "PRIME-TransposeMirrorX": "0", "445 Shutter-SupportsTriggering": "0", "470 Shutter-SupportsTriggering": "0", "XLIGHT Hub-Description": "XLIGHT hub", "PRIME-PreampOffLimit": "10000", "470 AO-Sequenceable": "1", "528 Shutter-MaxVolts": "5.0000", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "Core-XYStage": "XYStage", "470 AO-TriggerSampleRate": "100000", "PRIME-Timing-PreTriggerDelayNs": "24672000.0000", "528-DA Device": "528 Shutter", "Focus-Description": "Nikon TE2000 focus adapter", "FilterBlock-State": "5", "520 AO-Demo": "No", "COM3-DelayBetweenCharsMs": "0.0000", "470 Shutter-Volts": "0.0000", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "PRIME-PP 2 THRESHOLD": "125", "PRIME-PP 4 THRESHOLD": "75", "XYStage-Acceleration": "4.0000", "470 Shutter-Demo": "No", "PRIME-Name": "PMPCIECam00", "640-DA Device": "640 Shutter", "405 AO-Block voltage": "false", "ElapsedTime-ms": 1426985, "PRIME-Offset": "100", "445 AO-IOChannel": "Dev1/ao1", "640 AO-MaxVolts": "5.0000", "PositionIndex": 34, "PRIME-PP 1 ENABLED": "Yes", "COM1-BaudRate": "9600", "640-State": "0", "COM3-Verbose": "1", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "LudlController-TransmissionDelay": "0", "PRIME-FullWellCapacity": "62000", "PRIME-SMARTStreamingEnabled": "No", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "640 Shutter-Description": "NI DAC", "528-Description": "DA device that is used as a shutter", "COM4-Description": "Serial port driver (boost:asio)", "405 Shutter-Description": "NI DAC", "TE2000-Name": "TE2000", "528 Shutter-Description": "NI DAC", "COM9-Description": "Serial port driver (boost:asio)", "LudlController-Name": "LudlController", "445 Shutter-TriggerSequenceLength": "1000", "PRIME-SerialNumber": "A18B203008", "445 AO-Volts": "0.0000", "PRIME-X-dimension": "1200", "PRIME-ShutterCloseDelay": "0", "XLIGHT Disk slider-State": "2", "Position": "Default", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 AO-IOChannel": "Dev1/ao5", "520 AO-IOChannel": "Dev1/ao4", "528 AO-TriggerSequenceLength": "1000", "405 Shutter-MaxVolts": "5.0000", "470-DA Device": "470 Shutter", "PRIME-FTCapable": "No", "Core-ChannelGroup": "Channels", "555 AO-SupportsTriggering": "0", "PRIME-Y-dimension": "1200", "Frame": 0, "555 Shutter-Sequenceable": "1", "PRIME-Timing-ExposureTimeNs": "499998000.0000", "Core-ImageProcessor": "", "528-State": "0", "520 AO-Block voltage": "false", "XLIGHT Excitation Wheel-Label": "QUAD", "640 Shutter-Demo": "No", "COM3-StopBits": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "520 Shutter-IOChannel": "Dev1/ao11", "Width": 1200, "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-SupportsTriggering": "0", "640 AO-TriggerSampleRate": "100000", "520-Description": "DA device that is used as a shutter", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "405 Shutter-Sequenceable": "1", "405 Shutter-SupportsTriggering": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-TriggerSampleRate": "100000", "640 Shutter-MaxVolts": "5.0000", "555 AO-MaxVolts": "5.0000", "520 AO-TriggerSequenceLength": "1000", "COM3-BaudRate": "9600", "COM9-BaudRate": "9600", "Vincent-D3-Channel #": "Ch. #1", "PRIME-PMode": "Normal", "OpticalPath-Label": "Left(100)", "BitDepth": 16, "555 AO-TriggerSequenceLength": "1000", "445 Shutter-Description": "NI DAC", "405 AO-Sequenceable": "1", "PRIME-AcquisitionMethod": "Callbacks", "555 AO-Name": "AnalogIO", "PRIME-ShutterMode": "Pre-Exposure", "COM3-Handshaking": "Off", "445 AO-SupportsTriggering": "0", "ROI": "0-0-1200-1200", "COM9-Verbose": "1", "PRIME-Color": "OFF", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "PRIME-ShutterOpenDelay": "0", "COM1-Parity": "None", "Slice": 7, "SlicePosition": 20, "COM9-DelayBetweenCharsMs": "0.0000", "555-Name": "DA Shutter", "PIEZO-LudlSingleAxisName": "B", "XLIGHT Emission Wheel-Label": "QUAD", "640 Shutter-Name": "AnalogIO", "640 AO-Sequenceable": "1", "520 Shutter-Demo": "No", "528 Shutter-Demo": "No", "470-Name": "DA Shutter", "520-DA Device": "520 Shutter", "XYStage-Description": "Ludl XY stage driver adapter", "445 Shutter-MinVolts": "0.0000", "528 AO-TriggerSampleRate": "100000", "405 AO-MinVolts": "0.0000", "405 Shutter-TriggerSequenceLength": "1000", "Height": 1200, "555 AO-Block voltage": "false", "640 Shutter-Volts": "0.0000", "PixelType": "GRAY16", "445 Shutter-Block voltage": "false", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "520 Shutter-TriggerSequenceLength": "1000", "COM9-Name": "COM9", "Core-SLM": "", "555 AO-Volts": "0.0000", "555 Shutter-Block voltage": "false", "XYStage-Speed": "1800.0000", "520 AO-MaxVolts": "5.0000", "528 Shutter-SupportsTriggering": "0", "COM1-Description": "Serial port driver (boost:asio)", "PRIME-MetadataEnabled": "No", "520 Shutter-MinVolts": "0.0000", "470 Shutter-Sequenceable": "1", "PRIME-PreampDelay": "0", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-MinVolts": "0.0000", "ChannelIndex": 5, "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Core-AutoShutter": "1", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "Exposure-ms": 300, "PRIME-PP 2 ENABLED": "Yes", "640 Shutter-TriggerSampleRate": "100000", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "470 Shutter-IOChannel": "Dev1/ao10", "405 AO-Demo": "No", "PixelSizeUm": 1.1, "470 AO-Demo": "No", "470 Shutter-MaxVolts": "5.0000", "Vincent-D3-Name": "Vincent-D3", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FileName": "img_000000000_P-DAPI-high_007.tif", "LudlController-Reset": "Operate", "PRIME-Exposure": "300.00", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "445-Name": "DA Shutter", "555 Shutter-Name": "AnalogIO", "520 Shutter-TriggerSampleRate": "100000", "445 Shutter-IOChannel": "Dev1/ao9", "PRIME-TransposeCorrection": "0", "XYStage-StepSize": "0.1000", "445-Description": "DA device that is used as a shutter", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 AO-IOChannel": "Dev1/ao7", "520 Shutter-Name": "AnalogIO", "COM3-DataBits": "8", "640 AO-Volts": "0.0000", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "555 Shutter-IOChannel": "Dev1/ao13", "WaitInterval": null, "520 Shutter-Sequenceable": "1", "YPositionUm": 545.6, "445-State": "0", "445-DA Device": "445 Shutter", "PRIME-CircularBufferAutoSize": "ON", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "555 AO-TriggerSampleRate": "100000", "445 Shutter-Volts": "0.0000", "XLIGHT Hub-Port": "COM3", "Core-AutoFocus": "", "COM4-Handshaking": "Off", "528 Shutter-MinVolts": "0.0000", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "445 Shutter-Name": "AnalogIO", "528 Shutter-Sequenceable": "1", "XYStage-TransposeMirrorX": "0", "XYStage-TransposeMirrorY": "0", "PRIME-ReadoutRate": "100MHz 16bit", "XYStage-Name": "Stage", "640 AO-SupportsTriggering": "0", "640-Name": "DA Shutter", "640 Shutter-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "640 AO-Block voltage": "false", "445 Shutter-Sequenceable": "1", "470 AO-MaxVolts": "5.0000", "COM4-BaudRate": "9600", "470-Description": "DA device that is used as a shutter", "520 Shutter-Description": "NI DAC", "PRIME-CCDTemperature": "-14.8500", "Time": "2019-12-19 09:59:57 -0500", "640 AO-TriggerSequenceLength": "1000", "470 AO-Name": "AnalogIO", "XLIGHT Disk slider-Label": "Disk Pos 40um", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "Binning": "1x1", "TE2000-ModelType": "1", "COM3-AnswerTimeout": "500.0000", "555 Shutter-SupportsTriggering": "0", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "TE2000-Port": "COM4", "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Description": "NI DAC", "COM4-AnswerTimeout": "500.0000", "COM1-DelayBetweenCharsMs": "0.0000", "FilterBlock-Label": "Confocal", "445 AO-Name": "AnalogIO", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-Timing-ReadoutTimeNs": "24672000.0000", "528 AO-Demo": "No", "520 AO-Sequenceable": "1", "555-Description": "DA device that is used as a shutter", "405-DA Device": "405 Shutter", "405 AO-TriggerSequenceLength": "1000", "640 AO-MinVolts": "0.0000", "640 Shutter-Block voltage": "false", "FrameIndex": 0, "PRIME-Port": "CMOS", "555 AO-MinVolts": "0.0000", "COM3-Description": "Serial port driver (boost:asio)", "528 AO-Description": "NI DAC", "555 AO-IOChannel": "Dev1/ao6", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "555 AO-TriggerInputLine": "/Dev1/PFI0", "PRIME-ActualInterval-ms": "390.5200", "LudlController-Port": "COM9", "445 Shutter-Demo": "No", "PRIME-Trigger-Expose Out-Mux": "1", "SliceIndex": 7, "PRIME-CCDTemperatureSetPoint": "-15.0000", "405 Shutter-MinVolts": "0.0000", "PRIME-ExposeOutMode": "First Row", "AxisPositions": null, "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Volts": "0.0000", "528-Name": "DA Shutter", "555-State": "0", "XLIGHT Dichroic-Label": "QUAD", "445 AO-Sequenceable": "1", "405-Name": "DA Shutter", "405 AO-IOChannel": "Dev1/ao0", "Core-Camera": "PRIME", "Focus-Name": "Focus", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "405 Shutter-IOChannel": "Dev1/ao8", "640 AO-Demo": "No", "640 Shutter-SupportsTriggering": "0", "445 AO-Demo": "No", "555-DA Device": "555 Shutter", "470 Shutter-Description": "NI DAC", "528 AO-Volts": "0.0000", "COM1-DataBits": "8", "Vincent-D3-Address": "x", "520 AO-SupportsTriggering": "0", "405-Description": "DA device that is used as a shutter", "640 Shutter-MinVolts": "0.0000", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "405 AO-Name": "AnalogIO", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "640 AO-TriggerInputLine": "/Dev1/PFI0", "405 AO-TriggerInputLine": "/Dev1/PFI0", "640-Description": "DA device that is used as a shutter", "445 Shutter-TriggerSampleRate": "100000", "470 Shutter-TriggerSequenceLength": "1000", "COM1-StopBits": "1", "520 Shutter-SupportsTriggering": "0", "405-State": "1", "Core-Initialize": "1", "XLIGHT Emission Wheel-State": "3", "520 AO-Description": "NI DAC", "528 AO-TriggerInputLine": "/Dev1/PFI0", "445 Shutter-MaxVolts": "5.0000", "COM9-Handshaking": "Off", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "XLIGHT Spin motor-Label": "On (spinning)", "PRIME-PP 5 ENABLED": "No", "UUID": "7ce2433c-c326-42d7-9ece-511880635529", "PRIME-TriggerMode": "Internal Trigger", "COM1-Handshaking": "Off", "PRIME-Timing-PostTriggerDelayNs": "49344000.0000", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "Focus-Position": "17741.5000", "528 Shutter-Block voltage": "false", "Channel": "P-DAPI-high", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "555 Shutter-Demo": "No", "405 Shutter-Demo": "No", "528 AO-Name": "AnalogIO", "520-State": "0", "Core-Galvo": "", "405 Shutter-TriggerSampleRate": "100000", "Focus-FocusStepSize_(nm)": "100", "PRIME-PixelType": "16bit", "COM4-DataBits": "8", "470 AO-IOChannel": "Dev1/ao2", "520 AO-MinVolts": "0.0000", "COM9-Parity": "None", "PRIME-CircularBufferEnabled": "ON", "PRIME-PP 6 Reset": "No", "640 AO-Description": "NI DAC", "Source": "PRIME", "445 AO-MinVolts": "0.0000", "PIEZO-Name": "Stage", "445 AO-Description": "NI DAC", "PRIME-CircularBufferFrameCount": "46", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "520 AO-Volts": "0.0000", "528 Shutter-TriggerSampleRate": "100000", "XPositionUm": -1324.9, "OpticalPath-State": "4", "PRIME-PP 3 THRESHOLD": "80", "PRIME-PVCAM Version": "3.7.1", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Gain": "1-HDR", "640 Shutter-TriggerSequenceLength": "1000", "528 AO-SupportsTriggering": "0", "445 AO-TriggerInputLine": "/Dev1/PFI0", "OpticalPath-Name": "OpticalPath", "PRIME-PP 4 ENABLED": "Yes", "PRIME-PVCAM Adapter Version": "1.3.29", "COM3-Name": "COM3", "405 AO-MaxVolts": "5.0000", "520 Shutter-Block voltage": "false", "PRIME-Timing-ClearingTimeNs": "49344000.0000", "Core-Focus": "PIEZO", "528 AO-Block voltage": "false", "TE2000-Description": "Nikon TE2000 hub", "528 AO-MinVolts": "0.0000", "COM4-Parity": "None", "Summary": { "Slices": 11, "ChContrastMin": [ 0, 0, 0, 0, 0, 0 ], "PixelAspect": 1, "SlicesFirst": true, "Interval_ms": 0, "Time": "2019-12-19 09:59:21 -0500", "Prefix": "seq_13", "InitialPositionList": [ { "Label": "Pos0", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.21], "Focus": [17741.5], "XYStage": [ 44542.200000000004, 31636.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos1", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.22], "Focus": [17741.4], "XYStage": [ 43801.100000000006, 31637.9 ] }, "GridColumnIndex": 0 }, { "Label": "Pos2", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.5], "XYStage": [ 38561.3, 32040.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos3", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.185], "Focus": [17741.3], "XYStage": [ 37698.4, 32041.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos4", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.16], "Focus": [17741.5], "XYStage": [ 32375.4, 31758.9 ] }, "GridColumnIndex": 0 }, { "Label": "Pos5", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.17000000000002], "Focus": [17741.3], "XYStage": [ 31770.7, 31758.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos6", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.4], "XYStage": [ 26536.800000000003, 31068.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos7", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.205], "Focus": [17741.4], "XYStage": [ 25420.2, 31068.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos8", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.85], "Focus": [17741.3], "XYStage": [ 21059, 31508 ] }, "GridColumnIndex": 0 }, { "Label": "Pos9", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.82], "Focus": [17741.3], "XYStage": [ 19731.9, 31506.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos10", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.79], "Focus": [17741.4], "XYStage": [ 14528.5, 32007.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos11", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.83], "Focus": [17741.2], "XYStage": [ 13830.300000000001, 32009.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos12", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.79], "Focus": [17741.3], "XYStage": [ 8775.300000000001, 32017.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos13", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.815], "Focus": [17741.3], "XYStage": [ 8109.200000000001, 32008.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos14", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.805], "Focus": [17741.3], "XYStage": [ 3483.3, 31908.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos15", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.82], "Focus": [17741.2], "XYStage": [ 2686.8, 31903.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos16", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [211.48000000000002], "Focus": [17741.1], "XYStage": [ -1978.9, 31973.600000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos17", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [211.51500000000001], "Focus": [17741.1], "XYStage": [ -2957.8, 31982.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos18", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.195], "Focus": [17741.5], "XYStage": [ 47747.4, -576.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos19", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.23000000000002], "Focus": [17741.6], "XYStage": [ 47053.9, -576.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos20", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.205], "Focus": [17741.4], "XYStage": [ 41871.200000000004, -479.8 ] }, "GridColumnIndex": 0 }, { "Label": "Pos21", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.4], "XYStage": [ 40882.600000000006, -392 ] }, "GridColumnIndex": 0 }, { "Label": "Pos22", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.24], "Focus": [17741.4], "XYStage": [ 35905.6, -135.3 ] }, "GridColumnIndex": 0 }, { "Label": "Pos23", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.195], "Focus": [17741.5], "XYStage": [ 34990.9, -132.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos24", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.46], "Focus": [17741.4], "XYStage": [ 29678.100000000002, -958.1 ] }, "GridColumnIndex": 0 }, { "Label": "Pos25", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.44], "Focus": [17741.5], "XYStage": [ 28983.600000000002, -939.4000000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos26", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.475], "Focus": [17741.5], "XYStage": [ 23019.7, -122 ] }, "GridColumnIndex": 0 }, { "Label": "Pos27", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.44], "Focus": [17741.7], "XYStage": [ 22428, -116.80000000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos28", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.46], "Focus": [17741.7], "XYStage": [ 16364.900000000001, -181.10000000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos29", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.48000000000002], "Focus": [17741.5], "XYStage": [ 15712.1, -180 ] }, "GridColumnIndex": 0 }, { "Label": "Pos30", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.45000000000002], "Focus": [17741.7], "XYStage": [ 10346.1, 77.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos31", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.49], "Focus": [17741.4], "XYStage": [ 9755.5, 85.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos32", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.475], "Focus": [17741.5], "XYStage": [ 4775.5, 293.90000000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos33", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.445], "Focus": [17741.4], "XYStage": [ 3954.9, 293.90000000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos34", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.455], "Focus": [17741.4], "XYStage": [ -1324.9, 545.6 ] }, "GridColumnIndex": 0 }, { "Label": "Pos35", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.445], "Focus": [17741.3], "XYStage": [ -2097, 576.6 ] }, "GridColumnIndex": 0 } ], "z-step_um": 10, "GridRow": 0, "BitDepth": 16, "Depth": 2, "Channels": 6, "Source": "Micro-Manager", "ChColors": [ -16711681, -256, -65281, -1, -1, -16776961 ], "MetadataVersion": 10, "KeepShutterOpenChannels": true, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536, 65536 ], "CustomIntervals_ms": [], "Height": 1200, "MicroManagerVersion": "1.4.23 20180219", "GridColumn": 0, "UUID": "18808e7c-f2e6-47b3-90d8-1d779ce87dbd", "PixelType": "GRAY16", "Width": 1200, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\Yan\\20191218-YC113-S7S8-miseq", "Frames": 1, "Comment": "", "UserName": "Nikon", "PixelSize_um": 1.1, "ROI": [ 0, 0, 1200, 1200 ], "Date": "2019-12-19", "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC", "P-DAPI-high" ], "KeepShutterOpenSlices": true, "IJType": 1, "ComputerName": "ZadorConfocal2", "Positions": 36, "PositionIndex": 34, "CameraTimeout": 20000, "TimeFirst": false }, "640 AO-Name": "AnalogIO", "Vincent-D3-Command": "Close" }, "FrameKey-0-5-8": { "COM4-StopBits": "1", "528 AO-MaxVolts": "5.0000", "XLIGHT Excitation Wheel-State": "1", "470 Shutter-Name": "AnalogIO", "Core-Shutter": "405", "555 AO-Demo": "No", "XLIGHT Dichroic-State": "2", "XYStage-StepSize-Y": "0.1000", "ZPositionUm": 248.455, "XYStage-StepSize-X": "0.1000", "PRIME-FirmwareVersion": "20.28", "PRIME-ClearCycles": "2", "520-Name": "DA Shutter", "405 Shutter-Name": "AnalogIO", "PIEZO-StepSize": "0.0050", "PRIME-ClearMode": "Pre-Exposure", "470 AO-MinVolts": "0.0000", "405 AO-SupportsTriggering": "1", "CameraChannelIndex": 0, "XYStage-StartSpeed": "500.0000", "COM9-StopBits": "2", "405 AO-Description": "NI DAC", "COM4-DelayBetweenCharsMs": "0.0000", "445 AO-Block voltage": "false", "520 AO-TriggerInputLine": "/Dev1/PFI0", "555 AO-Description": "NI DAC", "520 AO-TriggerSampleRate": "100000", "COM4-Verbose": "1", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM4-Name": "COM4", "405 Shutter-Volts": "5.0000", "Vincent-D3-Port": "COM1", "PRIME-PP 4 MIN ADU AFFECTED": "200", "470 Shutter-MinVolts": "0.0000", "PRIME-PP 3 ENABLED": "Yes", "470 AO-Description": "NI DAC", "PRIME-Description": "PVCAM API device adapter", "520 Shutter-MaxVolts": "5.0000", "520 Shutter-Volts": "0.0000", "528 AO-Sequenceable": "1", "PRIME-Binning": "1x1", "555 Shutter-MaxVolts": "5.0000", "528 Shutter-TriggerSequenceLength": "1000", "405 Shutter-Block voltage": "false", "XLIGHT Spin motor-State": "1", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "Vincent-D3-Time to open (ms)": "35.0000", "Vincent-D3-Time to close (ms)": "35.0000", "445 AO-TriggerSampleRate": "100000", "640 Shutter-IOChannel": "Dev1/ao14", "555 AO-Sequenceable": "1", "520 AO-Name": "AnalogIO", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "470 AO-Block voltage": "false", "COM1-Verbose": "1", "COM9-AnswerTimeout": "10000.0000", "528 Shutter-Name": "AnalogIO", "PRIME-Trigger Timeout (secs)": "10", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "NextFrame": 0, "470 AO-TriggerSequenceLength": "1000", "PRIME-TransposeXY": "0", "445 AO-TriggerSequenceLength": "1000", "COM1-Name": "COM1", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "COM9-DataBits": "8", "COM1-AnswerTimeout": "500.0000", "Camera": "", "TE2000-Version": "V2.50-V2.50-V2.10", "528 Shutter-Volts": "0.0000", "XLIGHT Hub-Name": "XLIGHT Hub", "PIEZO-Description": "Ludl stage driver adapter", "PositionName": "Pos34", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSampleRate": "100000", "PRIME-FanSpeedSetpoint": "High", "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "405 AO-TriggerSampleRate": "100000", "470 Shutter-Block voltage": "false", "470-State": "0", "COM3-Parity": "None", "405 AO-Volts": "4.5000", "PRIME-TransposeMirrorY": "0", "PRIME-ChipName": "GS144BSI", "470 AO-Volts": "0.0000", "PRIME-TransposeMirrorX": "0", "445 Shutter-SupportsTriggering": "0", "470 Shutter-SupportsTriggering": "0", "XLIGHT Hub-Description": "XLIGHT hub", "PRIME-PreampOffLimit": "10000", "470 AO-Sequenceable": "1", "528 Shutter-MaxVolts": "5.0000", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "Core-XYStage": "XYStage", "470 AO-TriggerSampleRate": "100000", "PRIME-Timing-PreTriggerDelayNs": "24672000.0000", "528-DA Device": "528 Shutter", "Focus-Description": "Nikon TE2000 focus adapter", "FilterBlock-State": "5", "520 AO-Demo": "No", "COM3-DelayBetweenCharsMs": "0.0000", "470 Shutter-Volts": "0.0000", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "PRIME-PP 2 THRESHOLD": "125", "PRIME-PP 4 THRESHOLD": "75", "XYStage-Acceleration": "4.0000", "470 Shutter-Demo": "No", "PRIME-Name": "PMPCIECam00", "640-DA Device": "640 Shutter", "405 AO-Block voltage": "false", "ElapsedTime-ms": 1427539, "PRIME-Offset": "100", "445 AO-IOChannel": "Dev1/ao1", "640 AO-MaxVolts": "5.0000", "PositionIndex": 34, "PRIME-PP 1 ENABLED": "Yes", "COM1-BaudRate": "9600", "640-State": "0", "COM3-Verbose": "1", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "LudlController-TransmissionDelay": "0", "PRIME-FullWellCapacity": "62000", "PRIME-SMARTStreamingEnabled": "No", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "640 Shutter-Description": "NI DAC", "528-Description": "DA device that is used as a shutter", "COM4-Description": "Serial port driver (boost:asio)", "405 Shutter-Description": "NI DAC", "TE2000-Name": "TE2000", "528 Shutter-Description": "NI DAC", "COM9-Description": "Serial port driver (boost:asio)", "LudlController-Name": "LudlController", "445 Shutter-TriggerSequenceLength": "1000", "PRIME-SerialNumber": "A18B203008", "445 AO-Volts": "0.0000", "PRIME-X-dimension": "1200", "PRIME-ShutterCloseDelay": "0", "XLIGHT Disk slider-State": "2", "Position": "Default", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 AO-IOChannel": "Dev1/ao5", "520 AO-IOChannel": "Dev1/ao4", "528 AO-TriggerSequenceLength": "1000", "405 Shutter-MaxVolts": "5.0000", "470-DA Device": "470 Shutter", "PRIME-FTCapable": "No", "Core-ChannelGroup": "Channels", "555 AO-SupportsTriggering": "0", "PRIME-Y-dimension": "1200", "Frame": 0, "555 Shutter-Sequenceable": "1", "PRIME-Timing-ExposureTimeNs": "499998000.0000", "Core-ImageProcessor": "", "528-State": "0", "520 AO-Block voltage": "false", "XLIGHT Excitation Wheel-Label": "QUAD", "640 Shutter-Demo": "No", "COM3-StopBits": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "520 Shutter-IOChannel": "Dev1/ao11", "Width": 1200, "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-SupportsTriggering": "0", "640 AO-TriggerSampleRate": "100000", "520-Description": "DA device that is used as a shutter", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "405 Shutter-Sequenceable": "1", "405 Shutter-SupportsTriggering": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-TriggerSampleRate": "100000", "640 Shutter-MaxVolts": "5.0000", "555 AO-MaxVolts": "5.0000", "520 AO-TriggerSequenceLength": "1000", "COM3-BaudRate": "9600", "COM9-BaudRate": "9600", "Vincent-D3-Channel #": "Ch. #1", "PRIME-PMode": "Normal", "OpticalPath-Label": "Left(100)", "BitDepth": 16, "555 AO-TriggerSequenceLength": "1000", "445 Shutter-Description": "NI DAC", "405 AO-Sequenceable": "1", "PRIME-AcquisitionMethod": "Callbacks", "555 AO-Name": "AnalogIO", "PRIME-ShutterMode": "Pre-Exposure", "COM3-Handshaking": "Off", "445 AO-SupportsTriggering": "0", "ROI": "0-0-1200-1200", "COM9-Verbose": "1", "PRIME-Color": "OFF", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "PRIME-ShutterOpenDelay": "0", "COM1-Parity": "None", "Slice": 8, "SlicePosition": 30, "COM9-DelayBetweenCharsMs": "0.0000", "555-Name": "DA Shutter", "PIEZO-LudlSingleAxisName": "B", "XLIGHT Emission Wheel-Label": "QUAD", "640 Shutter-Name": "AnalogIO", "640 AO-Sequenceable": "1", "520 Shutter-Demo": "No", "528 Shutter-Demo": "No", "470-Name": "DA Shutter", "520-DA Device": "520 Shutter", "XYStage-Description": "Ludl XY stage driver adapter", "445 Shutter-MinVolts": "0.0000", "528 AO-TriggerSampleRate": "100000", "405 AO-MinVolts": "0.0000", "405 Shutter-TriggerSequenceLength": "1000", "Height": 1200, "555 AO-Block voltage": "false", "640 Shutter-Volts": "0.0000", "PixelType": "GRAY16", "445 Shutter-Block voltage": "false", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "520 Shutter-TriggerSequenceLength": "1000", "COM9-Name": "COM9", "Core-SLM": "", "555 AO-Volts": "0.0000", "555 Shutter-Block voltage": "false", "XYStage-Speed": "1800.0000", "520 AO-MaxVolts": "5.0000", "528 Shutter-SupportsTriggering": "0", "COM1-Description": "Serial port driver (boost:asio)", "PRIME-MetadataEnabled": "No", "520 Shutter-MinVolts": "0.0000", "470 Shutter-Sequenceable": "1", "PRIME-PreampDelay": "0", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-MinVolts": "0.0000", "ChannelIndex": 5, "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Core-AutoShutter": "1", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "Exposure-ms": 300, "PRIME-PP 2 ENABLED": "Yes", "640 Shutter-TriggerSampleRate": "100000", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "470 Shutter-IOChannel": "Dev1/ao10", "405 AO-Demo": "No", "PixelSizeUm": 1.1, "470 AO-Demo": "No", "470 Shutter-MaxVolts": "5.0000", "Vincent-D3-Name": "Vincent-D3", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FileName": "img_000000000_P-DAPI-high_008.tif", "LudlController-Reset": "Operate", "PRIME-Exposure": "300.00", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "445-Name": "DA Shutter", "555 Shutter-Name": "AnalogIO", "520 Shutter-TriggerSampleRate": "100000", "445 Shutter-IOChannel": "Dev1/ao9", "PRIME-TransposeCorrection": "0", "XYStage-StepSize": "0.1000", "445-Description": "DA device that is used as a shutter", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 AO-IOChannel": "Dev1/ao7", "520 Shutter-Name": "AnalogIO", "COM3-DataBits": "8", "640 AO-Volts": "0.0000", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "555 Shutter-IOChannel": "Dev1/ao13", "WaitInterval": null, "520 Shutter-Sequenceable": "1", "YPositionUm": 545.6, "445-State": "0", "445-DA Device": "445 Shutter", "PRIME-CircularBufferAutoSize": "ON", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "555 AO-TriggerSampleRate": "100000", "445 Shutter-Volts": "0.0000", "XLIGHT Hub-Port": "COM3", "Core-AutoFocus": "", "COM4-Handshaking": "Off", "528 Shutter-MinVolts": "0.0000", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "445 Shutter-Name": "AnalogIO", "528 Shutter-Sequenceable": "1", "XYStage-TransposeMirrorX": "0", "XYStage-TransposeMirrorY": "0", "PRIME-ReadoutRate": "100MHz 16bit", "XYStage-Name": "Stage", "640 AO-SupportsTriggering": "0", "640-Name": "DA Shutter", "640 Shutter-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "640 AO-Block voltage": "false", "445 Shutter-Sequenceable": "1", "470 AO-MaxVolts": "5.0000", "COM4-BaudRate": "9600", "470-Description": "DA device that is used as a shutter", "520 Shutter-Description": "NI DAC", "PRIME-CCDTemperature": "-14.8500", "Time": "2019-12-19 09:59:57 -0500", "640 AO-TriggerSequenceLength": "1000", "470 AO-Name": "AnalogIO", "XLIGHT Disk slider-Label": "Disk Pos 40um", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "Binning": "1x1", "TE2000-ModelType": "1", "COM3-AnswerTimeout": "500.0000", "555 Shutter-SupportsTriggering": "0", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "TE2000-Port": "COM4", "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Description": "NI DAC", "COM4-AnswerTimeout": "500.0000", "COM1-DelayBetweenCharsMs": "0.0000", "FilterBlock-Label": "Confocal", "445 AO-Name": "AnalogIO", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-Timing-ReadoutTimeNs": "24672000.0000", "528 AO-Demo": "No", "520 AO-Sequenceable": "1", "555-Description": "DA device that is used as a shutter", "405-DA Device": "405 Shutter", "405 AO-TriggerSequenceLength": "1000", "640 AO-MinVolts": "0.0000", "640 Shutter-Block voltage": "false", "FrameIndex": 0, "PRIME-Port": "CMOS", "555 AO-MinVolts": "0.0000", "COM3-Description": "Serial port driver (boost:asio)", "528 AO-Description": "NI DAC", "555 AO-IOChannel": "Dev1/ao6", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "555 AO-TriggerInputLine": "/Dev1/PFI0", "PRIME-ActualInterval-ms": "390.5200", "LudlController-Port": "COM9", "445 Shutter-Demo": "No", "PRIME-Trigger-Expose Out-Mux": "1", "SliceIndex": 8, "PRIME-CCDTemperatureSetPoint": "-15.0000", "405 Shutter-MinVolts": "0.0000", "PRIME-ExposeOutMode": "First Row", "AxisPositions": null, "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Volts": "0.0000", "528-Name": "DA Shutter", "555-State": "0", "XLIGHT Dichroic-Label": "QUAD", "445 AO-Sequenceable": "1", "405-Name": "DA Shutter", "405 AO-IOChannel": "Dev1/ao0", "Core-Camera": "PRIME", "Focus-Name": "Focus", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "405 Shutter-IOChannel": "Dev1/ao8", "640 AO-Demo": "No", "640 Shutter-SupportsTriggering": "0", "445 AO-Demo": "No", "555-DA Device": "555 Shutter", "470 Shutter-Description": "NI DAC", "528 AO-Volts": "0.0000", "COM1-DataBits": "8", "Vincent-D3-Address": "x", "520 AO-SupportsTriggering": "0", "405-Description": "DA device that is used as a shutter", "640 Shutter-MinVolts": "0.0000", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "405 AO-Name": "AnalogIO", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "640 AO-TriggerInputLine": "/Dev1/PFI0", "405 AO-TriggerInputLine": "/Dev1/PFI0", "640-Description": "DA device that is used as a shutter", "445 Shutter-TriggerSampleRate": "100000", "470 Shutter-TriggerSequenceLength": "1000", "COM1-StopBits": "1", "520 Shutter-SupportsTriggering": "0", "405-State": "1", "Core-Initialize": "1", "XLIGHT Emission Wheel-State": "3", "520 AO-Description": "NI DAC", "528 AO-TriggerInputLine": "/Dev1/PFI0", "445 Shutter-MaxVolts": "5.0000", "COM9-Handshaking": "Off", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "XLIGHT Spin motor-Label": "On (spinning)", "PRIME-PP 5 ENABLED": "No", "UUID": "646ef110-2d69-4c11-ace1-b89afd9e967e", "PRIME-TriggerMode": "Internal Trigger", "COM1-Handshaking": "Off", "PRIME-Timing-PostTriggerDelayNs": "49344000.0000", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "Focus-Position": "17741.5000", "528 Shutter-Block voltage": "false", "Channel": "P-DAPI-high", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "555 Shutter-Demo": "No", "405 Shutter-Demo": "No", "528 AO-Name": "AnalogIO", "520-State": "0", "Core-Galvo": "", "405 Shutter-TriggerSampleRate": "100000", "Focus-FocusStepSize_(nm)": "100", "PRIME-PixelType": "16bit", "COM4-DataBits": "8", "470 AO-IOChannel": "Dev1/ao2", "520 AO-MinVolts": "0.0000", "COM9-Parity": "None", "PRIME-CircularBufferEnabled": "ON", "PRIME-PP 6 Reset": "No", "640 AO-Description": "NI DAC", "Source": "PRIME", "445 AO-MinVolts": "0.0000", "PIEZO-Name": "Stage", "445 AO-Description": "NI DAC", "PRIME-CircularBufferFrameCount": "46", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "520 AO-Volts": "0.0000", "528 Shutter-TriggerSampleRate": "100000", "XPositionUm": -1324.9, "OpticalPath-State": "4", "PRIME-PP 3 THRESHOLD": "80", "PRIME-PVCAM Version": "3.7.1", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Gain": "1-HDR", "640 Shutter-TriggerSequenceLength": "1000", "528 AO-SupportsTriggering": "0", "445 AO-TriggerInputLine": "/Dev1/PFI0", "OpticalPath-Name": "OpticalPath", "PRIME-PP 4 ENABLED": "Yes", "PRIME-PVCAM Adapter Version": "1.3.29", "COM3-Name": "COM3", "405 AO-MaxVolts": "5.0000", "520 Shutter-Block voltage": "false", "PRIME-Timing-ClearingTimeNs": "49344000.0000", "Core-Focus": "PIEZO", "528 AO-Block voltage": "false", "TE2000-Description": "Nikon TE2000 hub", "528 AO-MinVolts": "0.0000", "COM4-Parity": "None", "Summary": { "Slices": 11, "ChContrastMin": [ 0, 0, 0, 0, 0, 0 ], "PixelAspect": 1, "SlicesFirst": true, "Interval_ms": 0, "Time": "2019-12-19 09:59:21 -0500", "Prefix": "seq_13", "InitialPositionList": [ { "Label": "Pos0", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.21], "Focus": [17741.5], "XYStage": [ 44542.200000000004, 31636.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos1", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.22], "Focus": [17741.4], "XYStage": [ 43801.100000000006, 31637.9 ] }, "GridColumnIndex": 0 }, { "Label": "Pos2", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.5], "XYStage": [ 38561.3, 32040.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos3", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.185], "Focus": [17741.3], "XYStage": [ 37698.4, 32041.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos4", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.16], "Focus": [17741.5], "XYStage": [ 32375.4, 31758.9 ] }, "GridColumnIndex": 0 }, { "Label": "Pos5", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.17000000000002], "Focus": [17741.3], "XYStage": [ 31770.7, 31758.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos6", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.4], "XYStage": [ 26536.800000000003, 31068.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos7", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.205], "Focus": [17741.4], "XYStage": [ 25420.2, 31068.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos8", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.85], "Focus": [17741.3], "XYStage": [ 21059, 31508 ] }, "GridColumnIndex": 0 }, { "Label": "Pos9", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.82], "Focus": [17741.3], "XYStage": [ 19731.9, 31506.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos10", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.79], "Focus": [17741.4], "XYStage": [ 14528.5, 32007.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos11", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.83], "Focus": [17741.2], "XYStage": [ 13830.300000000001, 32009.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos12", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.79], "Focus": [17741.3], "XYStage": [ 8775.300000000001, 32017.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos13", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.815], "Focus": [17741.3], "XYStage": [ 8109.200000000001, 32008.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos14", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.805], "Focus": [17741.3], "XYStage": [ 3483.3, 31908.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos15", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.82], "Focus": [17741.2], "XYStage": [ 2686.8, 31903.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos16", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [211.48000000000002], "Focus": [17741.1], "XYStage": [ -1978.9, 31973.600000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos17", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [211.51500000000001], "Focus": [17741.1], "XYStage": [ -2957.8, 31982.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos18", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.195], "Focus": [17741.5], "XYStage": [ 47747.4, -576.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos19", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.23000000000002], "Focus": [17741.6], "XYStage": [ 47053.9, -576.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos20", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.205], "Focus": [17741.4], "XYStage": [ 41871.200000000004, -479.8 ] }, "GridColumnIndex": 0 }, { "Label": "Pos21", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.4], "XYStage": [ 40882.600000000006, -392 ] }, "GridColumnIndex": 0 }, { "Label": "Pos22", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.24], "Focus": [17741.4], "XYStage": [ 35905.6, -135.3 ] }, "GridColumnIndex": 0 }, { "Label": "Pos23", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.195], "Focus": [17741.5], "XYStage": [ 34990.9, -132.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos24", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.46], "Focus": [17741.4], "XYStage": [ 29678.100000000002, -958.1 ] }, "GridColumnIndex": 0 }, { "Label": "Pos25", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.44], "Focus": [17741.5], "XYStage": [ 28983.600000000002, -939.4000000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos26", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.475], "Focus": [17741.5], "XYStage": [ 23019.7, -122 ] }, "GridColumnIndex": 0 }, { "Label": "Pos27", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.44], "Focus": [17741.7], "XYStage": [ 22428, -116.80000000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos28", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.46], "Focus": [17741.7], "XYStage": [ 16364.900000000001, -181.10000000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos29", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.48000000000002], "Focus": [17741.5], "XYStage": [ 15712.1, -180 ] }, "GridColumnIndex": 0 }, { "Label": "Pos30", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.45000000000002], "Focus": [17741.7], "XYStage": [ 10346.1, 77.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos31", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.49], "Focus": [17741.4], "XYStage": [ 9755.5, 85.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos32", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.475], "Focus": [17741.5], "XYStage": [ 4775.5, 293.90000000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos33", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.445], "Focus": [17741.4], "XYStage": [ 3954.9, 293.90000000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos34", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.455], "Focus": [17741.4], "XYStage": [ -1324.9, 545.6 ] }, "GridColumnIndex": 0 }, { "Label": "Pos35", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.445], "Focus": [17741.3], "XYStage": [ -2097, 576.6 ] }, "GridColumnIndex": 0 } ], "z-step_um": 10, "GridRow": 0, "BitDepth": 16, "Depth": 2, "Channels": 6, "Source": "Micro-Manager", "ChColors": [ -16711681, -256, -65281, -1, -1, -16776961 ], "MetadataVersion": 10, "KeepShutterOpenChannels": true, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536, 65536 ], "CustomIntervals_ms": [], "Height": 1200, "MicroManagerVersion": "1.4.23 20180219", "GridColumn": 0, "UUID": "18808e7c-f2e6-47b3-90d8-1d779ce87dbd", "PixelType": "GRAY16", "Width": 1200, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\Yan\\20191218-YC113-S7S8-miseq", "Frames": 1, "Comment": "", "UserName": "Nikon", "PixelSize_um": 1.1, "ROI": [ 0, 0, 1200, 1200 ], "Date": "2019-12-19", "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC", "P-DAPI-high" ], "KeepShutterOpenSlices": true, "IJType": 1, "ComputerName": "ZadorConfocal2", "Positions": 36, "PositionIndex": 34, "CameraTimeout": 20000, "TimeFirst": false }, "640 AO-Name": "AnalogIO", "Vincent-D3-Command": "Close" }, "FrameKey-0-5-9": { "COM4-StopBits": "1", "528 AO-MaxVolts": "5.0000", "XLIGHT Excitation Wheel-State": "1", "470 Shutter-Name": "AnalogIO", "Core-Shutter": "405", "555 AO-Demo": "No", "XLIGHT Dichroic-State": "2", "XYStage-StepSize-Y": "0.1000", "ZPositionUm": 258.45500000000004, "XYStage-StepSize-X": "0.1000", "PRIME-FirmwareVersion": "20.28", "PRIME-ClearCycles": "2", "520-Name": "DA Shutter", "405 Shutter-Name": "AnalogIO", "PIEZO-StepSize": "0.0050", "PRIME-ClearMode": "Pre-Exposure", "470 AO-MinVolts": "0.0000", "405 AO-SupportsTriggering": "1", "CameraChannelIndex": 0, "XYStage-StartSpeed": "500.0000", "COM9-StopBits": "2", "405 AO-Description": "NI DAC", "COM4-DelayBetweenCharsMs": "0.0000", "445 AO-Block voltage": "false", "520 AO-TriggerInputLine": "/Dev1/PFI0", "555 AO-Description": "NI DAC", "520 AO-TriggerSampleRate": "100000", "COM4-Verbose": "1", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM4-Name": "COM4", "405 Shutter-Volts": "5.0000", "Vincent-D3-Port": "COM1", "PRIME-PP 4 MIN ADU AFFECTED": "200", "470 Shutter-MinVolts": "0.0000", "PRIME-PP 3 ENABLED": "Yes", "470 AO-Description": "NI DAC", "PRIME-Description": "PVCAM API device adapter", "520 Shutter-MaxVolts": "5.0000", "520 Shutter-Volts": "0.0000", "528 AO-Sequenceable": "1", "PRIME-Binning": "1x1", "555 Shutter-MaxVolts": "5.0000", "528 Shutter-TriggerSequenceLength": "1000", "405 Shutter-Block voltage": "false", "XLIGHT Spin motor-State": "1", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "Vincent-D3-Time to open (ms)": "35.0000", "Vincent-D3-Time to close (ms)": "35.0000", "445 AO-TriggerSampleRate": "100000", "640 Shutter-IOChannel": "Dev1/ao14", "555 AO-Sequenceable": "1", "520 AO-Name": "AnalogIO", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "470 AO-Block voltage": "false", "COM1-Verbose": "1", "COM9-AnswerTimeout": "10000.0000", "528 Shutter-Name": "AnalogIO", "PRIME-Trigger Timeout (secs)": "10", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "NextFrame": 0, "470 AO-TriggerSequenceLength": "1000", "PRIME-TransposeXY": "0", "445 AO-TriggerSequenceLength": "1000", "COM1-Name": "COM1", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "COM9-DataBits": "8", "COM1-AnswerTimeout": "500.0000", "Camera": "", "TE2000-Version": "V2.50-V2.50-V2.10", "528 Shutter-Volts": "0.0000", "XLIGHT Hub-Name": "XLIGHT Hub", "PIEZO-Description": "Ludl stage driver adapter", "PositionName": "Pos34", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSampleRate": "100000", "PRIME-FanSpeedSetpoint": "High", "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "405 AO-TriggerSampleRate": "100000", "470 Shutter-Block voltage": "false", "470-State": "0", "COM3-Parity": "None", "405 AO-Volts": "4.5000", "PRIME-TransposeMirrorY": "0", "PRIME-ChipName": "GS144BSI", "470 AO-Volts": "0.0000", "PRIME-TransposeMirrorX": "0", "445 Shutter-SupportsTriggering": "0", "470 Shutter-SupportsTriggering": "0", "XLIGHT Hub-Description": "XLIGHT hub", "PRIME-PreampOffLimit": "10000", "470 AO-Sequenceable": "1", "528 Shutter-MaxVolts": "5.0000", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "Core-XYStage": "XYStage", "470 AO-TriggerSampleRate": "100000", "PRIME-Timing-PreTriggerDelayNs": "24672000.0000", "528-DA Device": "528 Shutter", "Focus-Description": "Nikon TE2000 focus adapter", "FilterBlock-State": "5", "520 AO-Demo": "No", "COM3-DelayBetweenCharsMs": "0.0000", "470 Shutter-Volts": "0.0000", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "PRIME-PP 2 THRESHOLD": "125", "PRIME-PP 4 THRESHOLD": "75", "XYStage-Acceleration": "4.0000", "470 Shutter-Demo": "No", "PRIME-Name": "PMPCIECam00", "640-DA Device": "640 Shutter", "405 AO-Block voltage": "false", "ElapsedTime-ms": 1428088, "PRIME-Offset": "100", "445 AO-IOChannel": "Dev1/ao1", "640 AO-MaxVolts": "5.0000", "PositionIndex": 34, "PRIME-PP 1 ENABLED": "Yes", "COM1-BaudRate": "9600", "640-State": "0", "COM3-Verbose": "1", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "LudlController-TransmissionDelay": "0", "PRIME-FullWellCapacity": "62000", "PRIME-SMARTStreamingEnabled": "No", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "640 Shutter-Description": "NI DAC", "528-Description": "DA device that is used as a shutter", "COM4-Description": "Serial port driver (boost:asio)", "405 Shutter-Description": "NI DAC", "TE2000-Name": "TE2000", "528 Shutter-Description": "NI DAC", "COM9-Description": "Serial port driver (boost:asio)", "LudlController-Name": "LudlController", "445 Shutter-TriggerSequenceLength": "1000", "PRIME-SerialNumber": "A18B203008", "445 AO-Volts": "0.0000", "PRIME-X-dimension": "1200", "PRIME-ShutterCloseDelay": "0", "XLIGHT Disk slider-State": "2", "Position": "Default", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 AO-IOChannel": "Dev1/ao5", "520 AO-IOChannel": "Dev1/ao4", "528 AO-TriggerSequenceLength": "1000", "405 Shutter-MaxVolts": "5.0000", "470-DA Device": "470 Shutter", "PRIME-FTCapable": "No", "Core-ChannelGroup": "Channels", "555 AO-SupportsTriggering": "0", "PRIME-Y-dimension": "1200", "Frame": 0, "555 Shutter-Sequenceable": "1", "PRIME-Timing-ExposureTimeNs": "499998000.0000", "Core-ImageProcessor": "", "528-State": "0", "520 AO-Block voltage": "false", "XLIGHT Excitation Wheel-Label": "QUAD", "640 Shutter-Demo": "No", "COM3-StopBits": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "520 Shutter-IOChannel": "Dev1/ao11", "Width": 1200, "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-SupportsTriggering": "0", "640 AO-TriggerSampleRate": "100000", "520-Description": "DA device that is used as a shutter", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "405 Shutter-Sequenceable": "1", "405 Shutter-SupportsTriggering": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-TriggerSampleRate": "100000", "640 Shutter-MaxVolts": "5.0000", "555 AO-MaxVolts": "5.0000", "520 AO-TriggerSequenceLength": "1000", "COM3-BaudRate": "9600", "COM9-BaudRate": "9600", "Vincent-D3-Channel #": "Ch. #1", "PRIME-PMode": "Normal", "OpticalPath-Label": "Left(100)", "BitDepth": 16, "555 AO-TriggerSequenceLength": "1000", "445 Shutter-Description": "NI DAC", "405 AO-Sequenceable": "1", "PRIME-AcquisitionMethod": "Callbacks", "555 AO-Name": "AnalogIO", "PRIME-ShutterMode": "Pre-Exposure", "COM3-Handshaking": "Off", "445 AO-SupportsTriggering": "0", "ROI": "0-0-1200-1200", "COM9-Verbose": "1", "PRIME-Color": "OFF", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "PRIME-ShutterOpenDelay": "0", "COM1-Parity": "None", "Slice": 9, "SlicePosition": 40, "COM9-DelayBetweenCharsMs": "0.0000", "555-Name": "DA Shutter", "PIEZO-LudlSingleAxisName": "B", "XLIGHT Emission Wheel-Label": "QUAD", "640 Shutter-Name": "AnalogIO", "640 AO-Sequenceable": "1", "520 Shutter-Demo": "No", "528 Shutter-Demo": "No", "470-Name": "DA Shutter", "520-DA Device": "520 Shutter", "XYStage-Description": "Ludl XY stage driver adapter", "445 Shutter-MinVolts": "0.0000", "528 AO-TriggerSampleRate": "100000", "405 AO-MinVolts": "0.0000", "405 Shutter-TriggerSequenceLength": "1000", "Height": 1200, "555 AO-Block voltage": "false", "640 Shutter-Volts": "0.0000", "PixelType": "GRAY16", "445 Shutter-Block voltage": "false", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "520 Shutter-TriggerSequenceLength": "1000", "COM9-Name": "COM9", "Core-SLM": "", "555 AO-Volts": "0.0000", "555 Shutter-Block voltage": "false", "XYStage-Speed": "1800.0000", "520 AO-MaxVolts": "5.0000", "528 Shutter-SupportsTriggering": "0", "COM1-Description": "Serial port driver (boost:asio)", "PRIME-MetadataEnabled": "No", "520 Shutter-MinVolts": "0.0000", "470 Shutter-Sequenceable": "1", "PRIME-PreampDelay": "0", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-MinVolts": "0.0000", "ChannelIndex": 5, "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Core-AutoShutter": "1", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "Exposure-ms": 300, "PRIME-PP 2 ENABLED": "Yes", "640 Shutter-TriggerSampleRate": "100000", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "470 Shutter-IOChannel": "Dev1/ao10", "405 AO-Demo": "No", "PixelSizeUm": 1.1, "470 AO-Demo": "No", "470 Shutter-MaxVolts": "5.0000", "Vincent-D3-Name": "Vincent-D3", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FileName": "img_000000000_P-DAPI-high_009.tif", "LudlController-Reset": "Operate", "PRIME-Exposure": "300.00", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "445-Name": "DA Shutter", "555 Shutter-Name": "AnalogIO", "520 Shutter-TriggerSampleRate": "100000", "445 Shutter-IOChannel": "Dev1/ao9", "PRIME-TransposeCorrection": "0", "XYStage-StepSize": "0.1000", "445-Description": "DA device that is used as a shutter", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 AO-IOChannel": "Dev1/ao7", "520 Shutter-Name": "AnalogIO", "COM3-DataBits": "8", "640 AO-Volts": "0.0000", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "555 Shutter-IOChannel": "Dev1/ao13", "WaitInterval": null, "520 Shutter-Sequenceable": "1", "YPositionUm": 545.6, "445-State": "0", "445-DA Device": "445 Shutter", "PRIME-CircularBufferAutoSize": "ON", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "555 AO-TriggerSampleRate": "100000", "445 Shutter-Volts": "0.0000", "XLIGHT Hub-Port": "COM3", "Core-AutoFocus": "", "COM4-Handshaking": "Off", "528 Shutter-MinVolts": "0.0000", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "445 Shutter-Name": "AnalogIO", "528 Shutter-Sequenceable": "1", "XYStage-TransposeMirrorX": "0", "XYStage-TransposeMirrorY": "0", "PRIME-ReadoutRate": "100MHz 16bit", "XYStage-Name": "Stage", "640 AO-SupportsTriggering": "0", "640-Name": "DA Shutter", "640 Shutter-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "640 AO-Block voltage": "false", "445 Shutter-Sequenceable": "1", "470 AO-MaxVolts": "5.0000", "COM4-BaudRate": "9600", "470-Description": "DA device that is used as a shutter", "520 Shutter-Description": "NI DAC", "PRIME-CCDTemperature": "-14.8500", "Time": "2019-12-19 09:59:58 -0500", "640 AO-TriggerSequenceLength": "1000", "470 AO-Name": "AnalogIO", "XLIGHT Disk slider-Label": "Disk Pos 40um", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "Binning": "1x1", "TE2000-ModelType": "1", "COM3-AnswerTimeout": "500.0000", "555 Shutter-SupportsTriggering": "0", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "TE2000-Port": "COM4", "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Description": "NI DAC", "COM4-AnswerTimeout": "500.0000", "COM1-DelayBetweenCharsMs": "0.0000", "FilterBlock-Label": "Confocal", "445 AO-Name": "AnalogIO", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-Timing-ReadoutTimeNs": "24672000.0000", "528 AO-Demo": "No", "520 AO-Sequenceable": "1", "555-Description": "DA device that is used as a shutter", "405-DA Device": "405 Shutter", "405 AO-TriggerSequenceLength": "1000", "640 AO-MinVolts": "0.0000", "640 Shutter-Block voltage": "false", "FrameIndex": 0, "PRIME-Port": "CMOS", "555 AO-MinVolts": "0.0000", "COM3-Description": "Serial port driver (boost:asio)", "528 AO-Description": "NI DAC", "555 AO-IOChannel": "Dev1/ao6", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "555 AO-TriggerInputLine": "/Dev1/PFI0", "PRIME-ActualInterval-ms": "390.5200", "LudlController-Port": "COM9", "445 Shutter-Demo": "No", "PRIME-Trigger-Expose Out-Mux": "1", "SliceIndex": 9, "PRIME-CCDTemperatureSetPoint": "-15.0000", "405 Shutter-MinVolts": "0.0000", "PRIME-ExposeOutMode": "First Row", "AxisPositions": null, "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Volts": "0.0000", "528-Name": "DA Shutter", "555-State": "0", "XLIGHT Dichroic-Label": "QUAD", "445 AO-Sequenceable": "1", "405-Name": "DA Shutter", "405 AO-IOChannel": "Dev1/ao0", "Core-Camera": "PRIME", "Focus-Name": "Focus", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "405 Shutter-IOChannel": "Dev1/ao8", "640 AO-Demo": "No", "640 Shutter-SupportsTriggering": "0", "445 AO-Demo": "No", "555-DA Device": "555 Shutter", "470 Shutter-Description": "NI DAC", "528 AO-Volts": "0.0000", "COM1-DataBits": "8", "Vincent-D3-Address": "x", "520 AO-SupportsTriggering": "0", "405-Description": "DA device that is used as a shutter", "640 Shutter-MinVolts": "0.0000", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "405 AO-Name": "AnalogIO", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "640 AO-TriggerInputLine": "/Dev1/PFI0", "405 AO-TriggerInputLine": "/Dev1/PFI0", "640-Description": "DA device that is used as a shutter", "445 Shutter-TriggerSampleRate": "100000", "470 Shutter-TriggerSequenceLength": "1000", "COM1-StopBits": "1", "520 Shutter-SupportsTriggering": "0", "405-State": "1", "Core-Initialize": "1", "XLIGHT Emission Wheel-State": "3", "520 AO-Description": "NI DAC", "528 AO-TriggerInputLine": "/Dev1/PFI0", "445 Shutter-MaxVolts": "5.0000", "COM9-Handshaking": "Off", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "XLIGHT Spin motor-Label": "On (spinning)", "PRIME-PP 5 ENABLED": "No", "UUID": "2fcc239b-0020-4f59-beff-c89293d52068", "PRIME-TriggerMode": "Internal Trigger", "COM1-Handshaking": "Off", "PRIME-Timing-PostTriggerDelayNs": "49344000.0000", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "Focus-Position": "17741.5000", "528 Shutter-Block voltage": "false", "Channel": "P-DAPI-high", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "555 Shutter-Demo": "No", "405 Shutter-Demo": "No", "528 AO-Name": "AnalogIO", "520-State": "0", "Core-Galvo": "", "405 Shutter-TriggerSampleRate": "100000", "Focus-FocusStepSize_(nm)": "100", "PRIME-PixelType": "16bit", "COM4-DataBits": "8", "470 AO-IOChannel": "Dev1/ao2", "520 AO-MinVolts": "0.0000", "COM9-Parity": "None", "PRIME-CircularBufferEnabled": "ON", "PRIME-PP 6 Reset": "No", "640 AO-Description": "NI DAC", "Source": "PRIME", "445 AO-MinVolts": "0.0000", "PIEZO-Name": "Stage", "445 AO-Description": "NI DAC", "PRIME-CircularBufferFrameCount": "46", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "520 AO-Volts": "0.0000", "528 Shutter-TriggerSampleRate": "100000", "XPositionUm": -1324.9, "OpticalPath-State": "4", "PRIME-PP 3 THRESHOLD": "80", "PRIME-PVCAM Version": "3.7.1", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Gain": "1-HDR", "640 Shutter-TriggerSequenceLength": "1000", "528 AO-SupportsTriggering": "0", "445 AO-TriggerInputLine": "/Dev1/PFI0", "OpticalPath-Name": "OpticalPath", "PRIME-PP 4 ENABLED": "Yes", "PRIME-PVCAM Adapter Version": "1.3.29", "COM3-Name": "COM3", "405 AO-MaxVolts": "5.0000", "520 Shutter-Block voltage": "false", "PRIME-Timing-ClearingTimeNs": "49344000.0000", "Core-Focus": "PIEZO", "528 AO-Block voltage": "false", "TE2000-Description": "Nikon TE2000 hub", "528 AO-MinVolts": "0.0000", "COM4-Parity": "None", "Summary": { "Slices": 11, "ChContrastMin": [ 0, 0, 0, 0, 0, 0 ], "PixelAspect": 1, "SlicesFirst": true, "Interval_ms": 0, "Time": "2019-12-19 09:59:21 -0500", "Prefix": "seq_13", "InitialPositionList": [ { "Label": "Pos0", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.21], "Focus": [17741.5], "XYStage": [ 44542.200000000004, 31636.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos1", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.22], "Focus": [17741.4], "XYStage": [ 43801.100000000006, 31637.9 ] }, "GridColumnIndex": 0 }, { "Label": "Pos2", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.5], "XYStage": [ 38561.3, 32040.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos3", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.185], "Focus": [17741.3], "XYStage": [ 37698.4, 32041.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos4", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.16], "Focus": [17741.5], "XYStage": [ 32375.4, 31758.9 ] }, "GridColumnIndex": 0 }, { "Label": "Pos5", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.17000000000002], "Focus": [17741.3], "XYStage": [ 31770.7, 31758.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos6", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.4], "XYStage": [ 26536.800000000003, 31068.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos7", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.205], "Focus": [17741.4], "XYStage": [ 25420.2, 31068.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos8", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.85], "Focus": [17741.3], "XYStage": [ 21059, 31508 ] }, "GridColumnIndex": 0 }, { "Label": "Pos9", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.82], "Focus": [17741.3], "XYStage": [ 19731.9, 31506.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos10", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.79], "Focus": [17741.4], "XYStage": [ 14528.5, 32007.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos11", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.83], "Focus": [17741.2], "XYStage": [ 13830.300000000001, 32009.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos12", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.79], "Focus": [17741.3], "XYStage": [ 8775.300000000001, 32017.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos13", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.815], "Focus": [17741.3], "XYStage": [ 8109.200000000001, 32008.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos14", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.805], "Focus": [17741.3], "XYStage": [ 3483.3, 31908.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos15", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.82], "Focus": [17741.2], "XYStage": [ 2686.8, 31903.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos16", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [211.48000000000002], "Focus": [17741.1], "XYStage": [ -1978.9, 31973.600000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos17", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [211.51500000000001], "Focus": [17741.1], "XYStage": [ -2957.8, 31982.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos18", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.195], "Focus": [17741.5], "XYStage": [ 47747.4, -576.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos19", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.23000000000002], "Focus": [17741.6], "XYStage": [ 47053.9, -576.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos20", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.205], "Focus": [17741.4], "XYStage": [ 41871.200000000004, -479.8 ] }, "GridColumnIndex": 0 }, { "Label": "Pos21", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.4], "XYStage": [ 40882.600000000006, -392 ] }, "GridColumnIndex": 0 }, { "Label": "Pos22", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.24], "Focus": [17741.4], "XYStage": [ 35905.6, -135.3 ] }, "GridColumnIndex": 0 }, { "Label": "Pos23", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.195], "Focus": [17741.5], "XYStage": [ 34990.9, -132.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos24", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.46], "Focus": [17741.4], "XYStage": [ 29678.100000000002, -958.1 ] }, "GridColumnIndex": 0 }, { "Label": "Pos25", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.44], "Focus": [17741.5], "XYStage": [ 28983.600000000002, -939.4000000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos26", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.475], "Focus": [17741.5], "XYStage": [ 23019.7, -122 ] }, "GridColumnIndex": 0 }, { "Label": "Pos27", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.44], "Focus": [17741.7], "XYStage": [ 22428, -116.80000000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos28", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.46], "Focus": [17741.7], "XYStage": [ 16364.900000000001, -181.10000000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos29", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.48000000000002], "Focus": [17741.5], "XYStage": [ 15712.1, -180 ] }, "GridColumnIndex": 0 }, { "Label": "Pos30", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.45000000000002], "Focus": [17741.7], "XYStage": [ 10346.1, 77.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos31", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.49], "Focus": [17741.4], "XYStage": [ 9755.5, 85.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos32", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.475], "Focus": [17741.5], "XYStage": [ 4775.5, 293.90000000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos33", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.445], "Focus": [17741.4], "XYStage": [ 3954.9, 293.90000000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos34", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.455], "Focus": [17741.4], "XYStage": [ -1324.9, 545.6 ] }, "GridColumnIndex": 0 }, { "Label": "Pos35", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.445], "Focus": [17741.3], "XYStage": [ -2097, 576.6 ] }, "GridColumnIndex": 0 } ], "z-step_um": 10, "GridRow": 0, "BitDepth": 16, "Depth": 2, "Channels": 6, "Source": "Micro-Manager", "ChColors": [ -16711681, -256, -65281, -1, -1, -16776961 ], "MetadataVersion": 10, "KeepShutterOpenChannels": true, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536, 65536 ], "CustomIntervals_ms": [], "Height": 1200, "MicroManagerVersion": "1.4.23 20180219", "GridColumn": 0, "UUID": "18808e7c-f2e6-47b3-90d8-1d779ce87dbd", "PixelType": "GRAY16", "Width": 1200, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\Yan\\20191218-YC113-S7S8-miseq", "Frames": 1, "Comment": "", "UserName": "Nikon", "PixelSize_um": 1.1, "ROI": [ 0, 0, 1200, 1200 ], "Date": "2019-12-19", "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC", "P-DAPI-high" ], "KeepShutterOpenSlices": true, "IJType": 1, "ComputerName": "ZadorConfocal2", "Positions": 36, "PositionIndex": 34, "CameraTimeout": 20000, "TimeFirst": false }, "640 AO-Name": "AnalogIO", "Vincent-D3-Command": "Close" }, "FrameKey-0-5-10": { "COM4-StopBits": "1", "528 AO-MaxVolts": "5.0000", "XLIGHT Excitation Wheel-State": "1", "470 Shutter-Name": "AnalogIO", "Core-Shutter": "405", "555 AO-Demo": "No", "XLIGHT Dichroic-State": "2", "XYStage-StepSize-Y": "0.1000", "ZPositionUm": 268.45500000000004, "XYStage-StepSize-X": "0.1000", "PRIME-FirmwareVersion": "20.28", "PRIME-ClearCycles": "2", "520-Name": "DA Shutter", "405 Shutter-Name": "AnalogIO", "PIEZO-StepSize": "0.0050", "PRIME-ClearMode": "Pre-Exposure", "470 AO-MinVolts": "0.0000", "405 AO-SupportsTriggering": "1", "CameraChannelIndex": 0, "XYStage-StartSpeed": "500.0000", "COM9-StopBits": "2", "405 AO-Description": "NI DAC", "COM4-DelayBetweenCharsMs": "0.0000", "445 AO-Block voltage": "false", "520 AO-TriggerInputLine": "/Dev1/PFI0", "555 AO-Description": "NI DAC", "520 AO-TriggerSampleRate": "100000", "COM4-Verbose": "1", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM4-Name": "COM4", "405 Shutter-Volts": "5.0000", "Vincent-D3-Port": "COM1", "PRIME-PP 4 MIN ADU AFFECTED": "200", "470 Shutter-MinVolts": "0.0000", "PRIME-PP 3 ENABLED": "Yes", "470 AO-Description": "NI DAC", "PRIME-Description": "PVCAM API device adapter", "520 Shutter-MaxVolts": "5.0000", "520 Shutter-Volts": "0.0000", "528 AO-Sequenceable": "1", "PRIME-Binning": "1x1", "555 Shutter-MaxVolts": "5.0000", "528 Shutter-TriggerSequenceLength": "1000", "405 Shutter-Block voltage": "false", "XLIGHT Spin motor-State": "1", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "Vincent-D3-Time to open (ms)": "35.0000", "Vincent-D3-Time to close (ms)": "35.0000", "445 AO-TriggerSampleRate": "100000", "640 Shutter-IOChannel": "Dev1/ao14", "555 AO-Sequenceable": "1", "520 AO-Name": "AnalogIO", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "470 AO-Block voltage": "false", "COM1-Verbose": "1", "COM9-AnswerTimeout": "10000.0000", "528 Shutter-Name": "AnalogIO", "PRIME-Trigger Timeout (secs)": "10", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "NextFrame": 0, "470 AO-TriggerSequenceLength": "1000", "PRIME-TransposeXY": "0", "445 AO-TriggerSequenceLength": "1000", "COM1-Name": "COM1", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "COM9-DataBits": "8", "COM1-AnswerTimeout": "500.0000", "Camera": "", "TE2000-Version": "V2.50-V2.50-V2.10", "528 Shutter-Volts": "0.0000", "XLIGHT Hub-Name": "XLIGHT Hub", "PIEZO-Description": "Ludl stage driver adapter", "PositionName": "Pos34", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSampleRate": "100000", "PRIME-FanSpeedSetpoint": "High", "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "405 AO-TriggerSampleRate": "100000", "470 Shutter-Block voltage": "false", "470-State": "0", "COM3-Parity": "None", "405 AO-Volts": "4.5000", "PRIME-TransposeMirrorY": "0", "PRIME-ChipName": "GS144BSI", "470 AO-Volts": "0.0000", "PRIME-TransposeMirrorX": "0", "445 Shutter-SupportsTriggering": "0", "470 Shutter-SupportsTriggering": "0", "XLIGHT Hub-Description": "XLIGHT hub", "PRIME-PreampOffLimit": "10000", "470 AO-Sequenceable": "1", "528 Shutter-MaxVolts": "5.0000", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "Core-XYStage": "XYStage", "470 AO-TriggerSampleRate": "100000", "PRIME-Timing-PreTriggerDelayNs": "24672000.0000", "528-DA Device": "528 Shutter", "Focus-Description": "Nikon TE2000 focus adapter", "FilterBlock-State": "5", "520 AO-Demo": "No", "COM3-DelayBetweenCharsMs": "0.0000", "470 Shutter-Volts": "0.0000", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "PRIME-PP 2 THRESHOLD": "125", "PRIME-PP 4 THRESHOLD": "75", "XYStage-Acceleration": "4.0000", "470 Shutter-Demo": "No", "PRIME-Name": "PMPCIECam00", "640-DA Device": "640 Shutter", "405 AO-Block voltage": "false", "ElapsedTime-ms": 1428645, "PRIME-Offset": "100", "445 AO-IOChannel": "Dev1/ao1", "640 AO-MaxVolts": "5.0000", "PositionIndex": 34, "PRIME-PP 1 ENABLED": "Yes", "COM1-BaudRate": "9600", "640-State": "0", "COM3-Verbose": "1", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "LudlController-TransmissionDelay": "0", "PRIME-FullWellCapacity": "62000", "PRIME-SMARTStreamingEnabled": "No", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "640 Shutter-Description": "NI DAC", "528-Description": "DA device that is used as a shutter", "COM4-Description": "Serial port driver (boost:asio)", "405 Shutter-Description": "NI DAC", "TE2000-Name": "TE2000", "528 Shutter-Description": "NI DAC", "COM9-Description": "Serial port driver (boost:asio)", "LudlController-Name": "LudlController", "445 Shutter-TriggerSequenceLength": "1000", "PRIME-SerialNumber": "A18B203008", "445 AO-Volts": "0.0000", "PRIME-X-dimension": "1200", "PRIME-ShutterCloseDelay": "0", "XLIGHT Disk slider-State": "2", "Position": "Default", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 AO-IOChannel": "Dev1/ao5", "520 AO-IOChannel": "Dev1/ao4", "528 AO-TriggerSequenceLength": "1000", "405 Shutter-MaxVolts": "5.0000", "470-DA Device": "470 Shutter", "PRIME-FTCapable": "No", "Core-ChannelGroup": "Channels", "555 AO-SupportsTriggering": "0", "PRIME-Y-dimension": "1200", "Frame": 0, "555 Shutter-Sequenceable": "1", "PRIME-Timing-ExposureTimeNs": "499998000.0000", "Core-ImageProcessor": "", "528-State": "0", "520 AO-Block voltage": "false", "XLIGHT Excitation Wheel-Label": "QUAD", "640 Shutter-Demo": "No", "COM3-StopBits": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "520 Shutter-IOChannel": "Dev1/ao11", "Width": 1200, "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-SupportsTriggering": "0", "640 AO-TriggerSampleRate": "100000", "520-Description": "DA device that is used as a shutter", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "405 Shutter-Sequenceable": "1", "405 Shutter-SupportsTriggering": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-TriggerSampleRate": "100000", "640 Shutter-MaxVolts": "5.0000", "555 AO-MaxVolts": "5.0000", "520 AO-TriggerSequenceLength": "1000", "COM3-BaudRate": "9600", "COM9-BaudRate": "9600", "Vincent-D3-Channel #": "Ch. #1", "PRIME-PMode": "Normal", "OpticalPath-Label": "Left(100)", "BitDepth": 16, "555 AO-TriggerSequenceLength": "1000", "445 Shutter-Description": "NI DAC", "405 AO-Sequenceable": "1", "PRIME-AcquisitionMethod": "Callbacks", "555 AO-Name": "AnalogIO", "PRIME-ShutterMode": "Pre-Exposure", "COM3-Handshaking": "Off", "445 AO-SupportsTriggering": "0", "ROI": "0-0-1200-1200", "COM9-Verbose": "1", "PRIME-Color": "OFF", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "PRIME-ShutterOpenDelay": "0", "COM1-Parity": "None", "Slice": 10, "SlicePosition": 50, "COM9-DelayBetweenCharsMs": "0.0000", "555-Name": "DA Shutter", "PIEZO-LudlSingleAxisName": "B", "XLIGHT Emission Wheel-Label": "QUAD", "640 Shutter-Name": "AnalogIO", "640 AO-Sequenceable": "1", "520 Shutter-Demo": "No", "528 Shutter-Demo": "No", "470-Name": "DA Shutter", "520-DA Device": "520 Shutter", "XYStage-Description": "Ludl XY stage driver adapter", "445 Shutter-MinVolts": "0.0000", "528 AO-TriggerSampleRate": "100000", "405 AO-MinVolts": "0.0000", "405 Shutter-TriggerSequenceLength": "1000", "Height": 1200, "555 AO-Block voltage": "false", "640 Shutter-Volts": "0.0000", "PixelType": "GRAY16", "445 Shutter-Block voltage": "false", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "520 Shutter-TriggerSequenceLength": "1000", "COM9-Name": "COM9", "Core-SLM": "", "555 AO-Volts": "0.0000", "555 Shutter-Block voltage": "false", "XYStage-Speed": "1800.0000", "520 AO-MaxVolts": "5.0000", "528 Shutter-SupportsTriggering": "0", "COM1-Description": "Serial port driver (boost:asio)", "PRIME-MetadataEnabled": "No", "520 Shutter-MinVolts": "0.0000", "470 Shutter-Sequenceable": "1", "PRIME-PreampDelay": "0", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-MinVolts": "0.0000", "ChannelIndex": 5, "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Core-AutoShutter": "1", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "Exposure-ms": 300, "PRIME-PP 2 ENABLED": "Yes", "640 Shutter-TriggerSampleRate": "100000", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "470 Shutter-IOChannel": "Dev1/ao10", "405 AO-Demo": "No", "PixelSizeUm": 1.1, "470 AO-Demo": "No", "470 Shutter-MaxVolts": "5.0000", "Vincent-D3-Name": "Vincent-D3", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FileName": "img_000000000_P-DAPI-high_010.tif", "LudlController-Reset": "Operate", "PRIME-Exposure": "300.00", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "445-Name": "DA Shutter", "555 Shutter-Name": "AnalogIO", "520 Shutter-TriggerSampleRate": "100000", "445 Shutter-IOChannel": "Dev1/ao9", "PRIME-TransposeCorrection": "0", "XYStage-StepSize": "0.1000", "445-Description": "DA device that is used as a shutter", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 AO-IOChannel": "Dev1/ao7", "520 Shutter-Name": "AnalogIO", "COM3-DataBits": "8", "640 AO-Volts": "0.0000", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "555 Shutter-IOChannel": "Dev1/ao13", "WaitInterval": null, "520 Shutter-Sequenceable": "1", "YPositionUm": 545.6, "445-State": "0", "445-DA Device": "445 Shutter", "PRIME-CircularBufferAutoSize": "ON", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "555 AO-TriggerSampleRate": "100000", "445 Shutter-Volts": "0.0000", "XLIGHT Hub-Port": "COM3", "Core-AutoFocus": "", "COM4-Handshaking": "Off", "528 Shutter-MinVolts": "0.0000", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "445 Shutter-Name": "AnalogIO", "528 Shutter-Sequenceable": "1", "XYStage-TransposeMirrorX": "0", "XYStage-TransposeMirrorY": "0", "PRIME-ReadoutRate": "100MHz 16bit", "XYStage-Name": "Stage", "640 AO-SupportsTriggering": "0", "640-Name": "DA Shutter", "640 Shutter-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "640 AO-Block voltage": "false", "445 Shutter-Sequenceable": "1", "470 AO-MaxVolts": "5.0000", "COM4-BaudRate": "9600", "470-Description": "DA device that is used as a shutter", "520 Shutter-Description": "NI DAC", "PRIME-CCDTemperature": "-14.8500", "Time": "2019-12-19 09:59:59 -0500", "640 AO-TriggerSequenceLength": "1000", "470 AO-Name": "AnalogIO", "XLIGHT Disk slider-Label": "Disk Pos 40um", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "Binning": "1x1", "TE2000-ModelType": "1", "COM3-AnswerTimeout": "500.0000", "555 Shutter-SupportsTriggering": "0", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "TE2000-Port": "COM4", "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Description": "NI DAC", "COM4-AnswerTimeout": "500.0000", "COM1-DelayBetweenCharsMs": "0.0000", "FilterBlock-Label": "Confocal", "445 AO-Name": "AnalogIO", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-Timing-ReadoutTimeNs": "24672000.0000", "528 AO-Demo": "No", "520 AO-Sequenceable": "1", "555-Description": "DA device that is used as a shutter", "405-DA Device": "405 Shutter", "405 AO-TriggerSequenceLength": "1000", "640 AO-MinVolts": "0.0000", "640 Shutter-Block voltage": "false", "FrameIndex": 0, "PRIME-Port": "CMOS", "555 AO-MinVolts": "0.0000", "COM3-Description": "Serial port driver (boost:asio)", "528 AO-Description": "NI DAC", "555 AO-IOChannel": "Dev1/ao6", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "555 AO-TriggerInputLine": "/Dev1/PFI0", "PRIME-ActualInterval-ms": "390.5200", "LudlController-Port": "COM9", "445 Shutter-Demo": "No", "PRIME-Trigger-Expose Out-Mux": "1", "SliceIndex": 10, "PRIME-CCDTemperatureSetPoint": "-15.0000", "405 Shutter-MinVolts": "0.0000", "PRIME-ExposeOutMode": "First Row", "AxisPositions": null, "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Volts": "0.0000", "528-Name": "DA Shutter", "555-State": "0", "XLIGHT Dichroic-Label": "QUAD", "445 AO-Sequenceable": "1", "405-Name": "DA Shutter", "405 AO-IOChannel": "Dev1/ao0", "Core-Camera": "PRIME", "Focus-Name": "Focus", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "405 Shutter-IOChannel": "Dev1/ao8", "640 AO-Demo": "No", "640 Shutter-SupportsTriggering": "0", "445 AO-Demo": "No", "555-DA Device": "555 Shutter", "470 Shutter-Description": "NI DAC", "528 AO-Volts": "0.0000", "COM1-DataBits": "8", "Vincent-D3-Address": "x", "520 AO-SupportsTriggering": "0", "405-Description": "DA device that is used as a shutter", "640 Shutter-MinVolts": "0.0000", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "405 AO-Name": "AnalogIO", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "640 AO-TriggerInputLine": "/Dev1/PFI0", "405 AO-TriggerInputLine": "/Dev1/PFI0", "640-Description": "DA device that is used as a shutter", "445 Shutter-TriggerSampleRate": "100000", "470 Shutter-TriggerSequenceLength": "1000", "COM1-StopBits": "1", "520 Shutter-SupportsTriggering": "0", "405-State": "0", "Core-Initialize": "1", "XLIGHT Emission Wheel-State": "3", "520 AO-Description": "NI DAC", "528 AO-TriggerInputLine": "/Dev1/PFI0", "445 Shutter-MaxVolts": "5.0000", "COM9-Handshaking": "Off", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "XLIGHT Spin motor-Label": "On (spinning)", "PRIME-PP 5 ENABLED": "No", "UUID": "3f1f18d2-2ffa-4a87-8d2b-5a70f7ce1746", "PRIME-TriggerMode": "Internal Trigger", "COM1-Handshaking": "Off", "PRIME-Timing-PostTriggerDelayNs": "49344000.0000", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "Focus-Position": "17741.5000", "528 Shutter-Block voltage": "false", "Channel": "P-DAPI-high", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "555 Shutter-Demo": "No", "405 Shutter-Demo": "No", "528 AO-Name": "AnalogIO", "520-State": "0", "Core-Galvo": "", "405 Shutter-TriggerSampleRate": "100000", "Focus-FocusStepSize_(nm)": "100", "PRIME-PixelType": "16bit", "COM4-DataBits": "8", "470 AO-IOChannel": "Dev1/ao2", "520 AO-MinVolts": "0.0000", "COM9-Parity": "None", "PRIME-CircularBufferEnabled": "ON", "PRIME-PP 6 Reset": "No", "640 AO-Description": "NI DAC", "Source": "PRIME", "445 AO-MinVolts": "0.0000", "PIEZO-Name": "Stage", "445 AO-Description": "NI DAC", "PRIME-CircularBufferFrameCount": "46", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "520 AO-Volts": "0.0000", "528 Shutter-TriggerSampleRate": "100000", "XPositionUm": -1324.9, "OpticalPath-State": "4", "PRIME-PP 3 THRESHOLD": "80", "PRIME-PVCAM Version": "3.7.1", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Gain": "1-HDR", "640 Shutter-TriggerSequenceLength": "1000", "528 AO-SupportsTriggering": "0", "445 AO-TriggerInputLine": "/Dev1/PFI0", "OpticalPath-Name": "OpticalPath", "PRIME-PP 4 ENABLED": "Yes", "PRIME-PVCAM Adapter Version": "1.3.29", "COM3-Name": "COM3", "405 AO-MaxVolts": "5.0000", "520 Shutter-Block voltage": "false", "PRIME-Timing-ClearingTimeNs": "49344000.0000", "Core-Focus": "PIEZO", "528 AO-Block voltage": "false", "TE2000-Description": "Nikon TE2000 hub", "528 AO-MinVolts": "0.0000", "COM4-Parity": "None", "Summary": { "Slices": 11, "ChContrastMin": [ 0, 0, 0, 0, 0, 0 ], "PixelAspect": 1, "SlicesFirst": true, "Interval_ms": 0, "Time": "2019-12-19 09:59:21 -0500", "Prefix": "seq_13", "InitialPositionList": [ { "Label": "Pos0", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.21], "Focus": [17741.5], "XYStage": [ 44542.200000000004, 31636.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos1", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.22], "Focus": [17741.4], "XYStage": [ 43801.100000000006, 31637.9 ] }, "GridColumnIndex": 0 }, { "Label": "Pos2", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.5], "XYStage": [ 38561.3, 32040.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos3", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.185], "Focus": [17741.3], "XYStage": [ 37698.4, 32041.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos4", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.16], "Focus": [17741.5], "XYStage": [ 32375.4, 31758.9 ] }, "GridColumnIndex": 0 }, { "Label": "Pos5", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.17000000000002], "Focus": [17741.3], "XYStage": [ 31770.7, 31758.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos6", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.4], "XYStage": [ 26536.800000000003, 31068.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos7", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.205], "Focus": [17741.4], "XYStage": [ 25420.2, 31068.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos8", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.85], "Focus": [17741.3], "XYStage": [ 21059, 31508 ] }, "GridColumnIndex": 0 }, { "Label": "Pos9", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.82], "Focus": [17741.3], "XYStage": [ 19731.9, 31506.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos10", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.79], "Focus": [17741.4], "XYStage": [ 14528.5, 32007.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos11", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.83], "Focus": [17741.2], "XYStage": [ 13830.300000000001, 32009.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos12", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.79], "Focus": [17741.3], "XYStage": [ 8775.300000000001, 32017.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos13", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.815], "Focus": [17741.3], "XYStage": [ 8109.200000000001, 32008.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos14", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.805], "Focus": [17741.3], "XYStage": [ 3483.3, 31908.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos15", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.82], "Focus": [17741.2], "XYStage": [ 2686.8, 31903.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos16", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [211.48000000000002], "Focus": [17741.1], "XYStage": [ -1978.9, 31973.600000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos17", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [211.51500000000001], "Focus": [17741.1], "XYStage": [ -2957.8, 31982.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos18", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.195], "Focus": [17741.5], "XYStage": [ 47747.4, -576.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos19", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.23000000000002], "Focus": [17741.6], "XYStage": [ 47053.9, -576.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos20", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.205], "Focus": [17741.4], "XYStage": [ 41871.200000000004, -479.8 ] }, "GridColumnIndex": 0 }, { "Label": "Pos21", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.4], "XYStage": [ 40882.600000000006, -392 ] }, "GridColumnIndex": 0 }, { "Label": "Pos22", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.24], "Focus": [17741.4], "XYStage": [ 35905.6, -135.3 ] }, "GridColumnIndex": 0 }, { "Label": "Pos23", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.195], "Focus": [17741.5], "XYStage": [ 34990.9, -132.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos24", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.46], "Focus": [17741.4], "XYStage": [ 29678.100000000002, -958.1 ] }, "GridColumnIndex": 0 }, { "Label": "Pos25", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.44], "Focus": [17741.5], "XYStage": [ 28983.600000000002, -939.4000000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos26", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.475], "Focus": [17741.5], "XYStage": [ 23019.7, -122 ] }, "GridColumnIndex": 0 }, { "Label": "Pos27", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.44], "Focus": [17741.7], "XYStage": [ 22428, -116.80000000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos28", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.46], "Focus": [17741.7], "XYStage": [ 16364.900000000001, -181.10000000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos29", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.48000000000002], "Focus": [17741.5], "XYStage": [ 15712.1, -180 ] }, "GridColumnIndex": 0 }, { "Label": "Pos30", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.45000000000002], "Focus": [17741.7], "XYStage": [ 10346.1, 77.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos31", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.49], "Focus": [17741.4], "XYStage": [ 9755.5, 85.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos32", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.475], "Focus": [17741.5], "XYStage": [ 4775.5, 293.90000000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos33", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.445], "Focus": [17741.4], "XYStage": [ 3954.9, 293.90000000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos34", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.455], "Focus": [17741.4], "XYStage": [ -1324.9, 545.6 ] }, "GridColumnIndex": 0 }, { "Label": "Pos35", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.445], "Focus": [17741.3], "XYStage": [ -2097, 576.6 ] }, "GridColumnIndex": 0 } ], "z-step_um": 10, "GridRow": 0, "BitDepth": 16, "Depth": 2, "Channels": 6, "Source": "Micro-Manager", "ChColors": [ -16711681, -256, -65281, -1, -1, -16776961 ], "MetadataVersion": 10, "KeepShutterOpenChannels": true, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536, 65536 ], "CustomIntervals_ms": [], "Height": 1200, "MicroManagerVersion": "1.4.23 20180219", "GridColumn": 0, "UUID": "18808e7c-f2e6-47b3-90d8-1d779ce87dbd", "PixelType": "GRAY16", "Width": 1200, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\Yan\\20191218-YC113-S7S8-miseq", "Frames": 1, "Comment": "", "UserName": "Nikon", "PixelSize_um": 1.1, "ROI": [ 0, 0, 1200, 1200 ], "Date": "2019-12-19", "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC", "P-DAPI-high" ], "KeepShutterOpenSlices": true, "IJType": 1, "ComputerName": "ZadorConfocal2", "Positions": 36, "PositionIndex": 34, "CameraTimeout": 20000, "TimeFirst": false }, "640 AO-Name": "AnalogIO", "Vincent-D3-Command": "Close" } }