{ "Summary": { "Slices": 11, "ChContrastMin": [ 0, 0, 0, 0, 0, 0 ], "PixelAspect": 1, "SlicesFirst": true, "Interval_ms": 0, "Time": "2019-12-18 21:36:42 -0500", "Prefix": "seq_4", "InitialPositionList": [ { "Label": "Pos0", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.21], "Focus": [17741.5], "XYStage": [ 44542.200000000004, 31636.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos1", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.22], "Focus": [17741.4], "XYStage": [ 43801.100000000006, 31637.9 ] }, "GridColumnIndex": 0 }, { "Label": "Pos2", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.5], "XYStage": [ 38561.3, 32040.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos3", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.185], "Focus": [17741.3], "XYStage": [ 37698.4, 32041.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos4", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.16], "Focus": [17741.5], "XYStage": [ 32375.4, 31758.9 ] }, "GridColumnIndex": 0 }, { "Label": "Pos5", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.17000000000002], "Focus": [17741.3], "XYStage": [ 31770.7, 31758.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos6", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.4], "XYStage": [ 26536.800000000003, 31068.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos7", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.205], "Focus": [17741.4], "XYStage": [ 25420.2, 31068.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos8", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.85], "Focus": [17741.3], "XYStage": [ 21059, 31508 ] }, "GridColumnIndex": 0 }, { "Label": "Pos9", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.82], "Focus": [17741.3], "XYStage": [ 19731.9, 31506.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos10", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.79], "Focus": [17741.4], "XYStage": [ 14528.5, 32007.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos11", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.83], "Focus": [17741.2], "XYStage": [ 13830.300000000001, 32009.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos12", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.79], "Focus": [17741.3], "XYStage": [ 8775.300000000001, 32017.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos13", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.815], "Focus": [17741.3], "XYStage": [ 8109.200000000001, 32008.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos14", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.805], "Focus": [17741.3], "XYStage": [ 3483.3, 31908.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos15", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.82], "Focus": [17741.2], "XYStage": [ 2686.8, 31903.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos16", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [211.48000000000002], "Focus": [17741.1], "XYStage": [ -1978.9, 31973.600000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos17", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [211.51500000000001], "Focus": [17741.1], "XYStage": [ -2957.8, 31982.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos18", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.195], "Focus": [17741.5], "XYStage": [ 47747.4, -576.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos19", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.23000000000002], "Focus": [17741.6], "XYStage": [ 47053.9, -576.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos20", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.205], "Focus": [17741.4], "XYStage": [ 41871.200000000004, -479.8 ] }, "GridColumnIndex": 0 }, { "Label": "Pos21", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.4], "XYStage": [ 40882.600000000006, -392 ] }, "GridColumnIndex": 0 }, { "Label": "Pos22", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.24], "Focus": [17741.4], "XYStage": [ 35905.6, -135.3 ] }, "GridColumnIndex": 0 }, { "Label": "Pos23", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.195], "Focus": [17741.5], "XYStage": [ 34990.9, -132.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos24", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.46], "Focus": [17741.4], "XYStage": [ 29678.100000000002, -958.1 ] }, "GridColumnIndex": 0 }, { "Label": "Pos25", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.44], "Focus": [17741.5], "XYStage": [ 28983.600000000002, -939.4000000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos26", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.475], "Focus": [17741.5], "XYStage": [ 23019.7, -122 ] }, "GridColumnIndex": 0 }, { "Label": "Pos27", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.44], "Focus": [17741.7], "XYStage": [ 22428, -116.80000000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos28", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.46], "Focus": [17741.7], "XYStage": [ 16364.900000000001, -181.10000000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos29", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.48000000000002], "Focus": [17741.5], "XYStage": [ 15712.1, -180 ] }, "GridColumnIndex": 0 }, { "Label": "Pos30", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.45000000000002], "Focus": [17741.7], "XYStage": [ 10346.1, 77.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos31", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.49], "Focus": [17741.4], "XYStage": [ 9755.5, 85.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos32", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.475], "Focus": [17741.5], "XYStage": [ 4775.5, 293.90000000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos33", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.445], "Focus": [17741.4], "XYStage": [ 3954.9, 293.90000000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos34", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.455], "Focus": [17741.4], "XYStage": [ -1324.9, 545.6 ] }, "GridColumnIndex": 0 }, { "Label": "Pos35", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.445], "Focus": [17741.3], "XYStage": [ -2097, 576.6 ] }, "GridColumnIndex": 0 } ], "z-step_um": 10, "GridRow": 0, "BitDepth": 16, "Depth": 2, "Channels": 6, "Source": "Micro-Manager", "ChColors": [ -16711681, -256, -65281, -1, -1, -16776961 ], "MetadataVersion": 10, "KeepShutterOpenChannels": true, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536, 65536 ], "CustomIntervals_ms": [], "Height": 1200, "MicroManagerVersion": "1.4.23 20180219", "GridColumn": 0, "UUID": "14a13bf7-c944-4672-b873-fcfbbb7ea13d", "PixelType": "GRAY16", "Width": 1200, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\Yan\\20191218-YC113-S7S8-miseq", "Frames": 1, "Comment": "", "UserName": "Nikon", "PixelSize_um": 1.1, "ROI": [ 0, 0, 1200, 1200 ], "Date": "2019-12-18", "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC", "P-DAPI-high" ], "KeepShutterOpenSlices": true, "IJType": 1, "ComputerName": "ZadorConfocal2", "Positions": 36, "PositionIndex": 9, "CameraTimeout": 20000, "TimeFirst": false }, "FrameKey-0-0-0": { "COM4-StopBits": "1", "528 AO-MaxVolts": "5.0000", "XLIGHT Excitation Wheel-State": "1", "470 Shutter-Name": "AnalogIO", "Core-Shutter": "520", "555 AO-Demo": "No", "XLIGHT Dichroic-State": "2", "XYStage-StepSize-Y": "0.1000", "ZPositionUm": 198.82, "XYStage-StepSize-X": "0.1000", "PRIME-FirmwareVersion": "20.28", "PRIME-ClearCycles": "2", "520-Name": "DA Shutter", "405 Shutter-Name": "AnalogIO", "PIEZO-StepSize": "0.0050", "PRIME-ClearMode": "Pre-Exposure", "470 AO-MinVolts": "0.0000", "405 AO-SupportsTriggering": "1", "CameraChannelIndex": 0, "XYStage-StartSpeed": "500.0000", "COM9-StopBits": "2", "405 AO-Description": "NI DAC", "COM4-DelayBetweenCharsMs": "0.0000", "445 AO-Block voltage": "false", "520 AO-TriggerInputLine": "/Dev1/PFI0", "555 AO-Description": "NI DAC", "520 AO-TriggerSampleRate": "100000", "COM4-Verbose": "1", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM4-Name": "COM4", "405 Shutter-Volts": "0.0000", "Vincent-D3-Port": "COM1", "PRIME-PP 4 MIN ADU AFFECTED": "200", "470 Shutter-MinVolts": "0.0000", "PRIME-PP 3 ENABLED": "Yes", "470 AO-Description": "NI DAC", "PRIME-Description": "PVCAM API device adapter", "520 Shutter-MaxVolts": "5.0000", "520 Shutter-Volts": "5.0000", "528 AO-Sequenceable": "1", "PRIME-Binning": "1x1", "555 Shutter-MaxVolts": "5.0000", "528 Shutter-TriggerSequenceLength": "1000", "405 Shutter-Block voltage": "false", "XLIGHT Spin motor-State": "1", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "Vincent-D3-Time to open (ms)": "35.0000", "Vincent-D3-Time to close (ms)": "35.0000", "445 AO-TriggerSampleRate": "100000", "640 Shutter-IOChannel": "Dev1/ao14", "555 AO-Sequenceable": "1", "520 AO-Name": "AnalogIO", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "470 AO-Block voltage": "false", "COM1-Verbose": "1", "COM9-AnswerTimeout": "10000.0000", "528 Shutter-Name": "AnalogIO", "PRIME-Trigger Timeout (secs)": "10", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "NextFrame": 0, "470 AO-TriggerSequenceLength": "1000", "PRIME-TransposeXY": "0", "445 AO-TriggerSequenceLength": "1000", "COM1-Name": "COM1", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "COM9-DataBits": "8", "COM1-AnswerTimeout": "500.0000", "Camera": "", "TE2000-Version": "V2.50-V2.50-V2.10", "528 Shutter-Volts": "0.0000", "XLIGHT Hub-Name": "XLIGHT Hub", "PIEZO-Description": "Ludl stage driver adapter", "PositionName": "Pos9", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSampleRate": "100000", "PRIME-FanSpeedSetpoint": "High", "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "405 AO-TriggerSampleRate": "100000", "470 Shutter-Block voltage": "false", "470-State": "0", "COM3-Parity": "None", "405 AO-Volts": "0.0000", "PRIME-TransposeMirrorY": "0", "PRIME-ChipName": "GS144BSI", "470 AO-Volts": "0.0000", "PRIME-TransposeMirrorX": "0", "445 Shutter-SupportsTriggering": "0", "470 Shutter-SupportsTriggering": "0", "XLIGHT Hub-Description": "XLIGHT hub", "PRIME-PreampOffLimit": "10000", "470 AO-Sequenceable": "1", "528 Shutter-MaxVolts": "5.0000", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "Core-XYStage": "XYStage", "470 AO-TriggerSampleRate": "100000", "PRIME-Timing-PreTriggerDelayNs": "24672000.0000", "528-DA Device": "528 Shutter", "Focus-Description": "Nikon TE2000 focus adapter", "FilterBlock-State": "5", "520 AO-Demo": "No", "COM3-DelayBetweenCharsMs": "0.0000", "470 Shutter-Volts": "0.0000", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "PRIME-PP 2 THRESHOLD": "125", "PRIME-PP 4 THRESHOLD": "75", "XYStage-Acceleration": "4.0000", "470 Shutter-Demo": "No", "PRIME-Name": "PMPCIECam00", "640-DA Device": "640 Shutter", "405 AO-Block voltage": "false", "ElapsedTime-ms": 374177, "PRIME-Offset": "100", "445 AO-IOChannel": "Dev1/ao1", "640 AO-MaxVolts": "5.0000", "PositionIndex": 9, "PRIME-PP 1 ENABLED": "Yes", "COM1-BaudRate": "9600", "640-State": "0", "COM3-Verbose": "1", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "LudlController-TransmissionDelay": "0", "PRIME-FullWellCapacity": "62000", "PRIME-SMARTStreamingEnabled": "No", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "640 Shutter-Description": "NI DAC", "528-Description": "DA device that is used as a shutter", "COM4-Description": "Serial port driver (boost:asio)", "405 Shutter-Description": "NI DAC", "TE2000-Name": "TE2000", "528 Shutter-Description": "NI DAC", "COM9-Description": "Serial port driver (boost:asio)", "LudlController-Name": "LudlController", "445 Shutter-TriggerSequenceLength": "1000", "PRIME-SerialNumber": "A18B203008", "445 AO-Volts": "0.0000", "PRIME-X-dimension": "1200", "PRIME-ShutterCloseDelay": "0", "XLIGHT Disk slider-State": "2", "Position": "Default", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 AO-IOChannel": "Dev1/ao5", "520 AO-IOChannel": "Dev1/ao4", "528 AO-TriggerSequenceLength": "1000", "405 Shutter-MaxVolts": "5.0000", "470-DA Device": "470 Shutter", "PRIME-FTCapable": "No", "Core-ChannelGroup": "Channels", "555 AO-SupportsTriggering": "0", "PRIME-Y-dimension": "1200", "Frame": 0, "555 Shutter-Sequenceable": "1", "PRIME-Timing-ExposureTimeNs": "499998000.0000", "Core-ImageProcessor": "", "528-State": "0", "520 AO-Block voltage": "false", "XLIGHT Excitation Wheel-Label": "CFP/YFP", "640 Shutter-Demo": "No", "COM3-StopBits": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "520 Shutter-IOChannel": "Dev1/ao11", "Width": 1200, "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-SupportsTriggering": "0", "640 AO-TriggerSampleRate": "100000", "520-Description": "DA device that is used as a shutter", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "405 Shutter-Sequenceable": "1", "405 Shutter-SupportsTriggering": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-TriggerSampleRate": "100000", "640 Shutter-MaxVolts": "5.0000", "555 AO-MaxVolts": "5.0000", "520 AO-TriggerSequenceLength": "1000", "COM3-BaudRate": "9600", "COM9-BaudRate": "9600", "Vincent-D3-Channel #": "Ch. #1", "PRIME-PMode": "Normal", "OpticalPath-Label": "Left(100)", "BitDepth": 16, "555 AO-TriggerSequenceLength": "1000", "445 Shutter-Description": "NI DAC", "405 AO-Sequenceable": "1", "PRIME-AcquisitionMethod": "Callbacks", "555 AO-Name": "AnalogIO", "PRIME-ShutterMode": "Pre-Exposure", "COM3-Handshaking": "Off", "445 AO-SupportsTriggering": "0", "ROI": "0-0-1200-1200", "COM9-Verbose": "1", "PRIME-Color": "OFF", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "PRIME-ShutterOpenDelay": "0", "COM1-Parity": "None", "Slice": 0, "SlicePosition": -50, "COM9-DelayBetweenCharsMs": "0.0000", "555-Name": "DA Shutter", "PIEZO-LudlSingleAxisName": "B", "XLIGHT Emission Wheel-Label": "565/24(G)", "640 Shutter-Name": "AnalogIO", "640 AO-Sequenceable": "1", "520 Shutter-Demo": "No", "528 Shutter-Demo": "No", "470-Name": "DA Shutter", "520-DA Device": "520 Shutter", "XYStage-Description": "Ludl XY stage driver adapter", "445 Shutter-MinVolts": "0.0000", "528 AO-TriggerSampleRate": "100000", "405 AO-MinVolts": "0.0000", "405 Shutter-TriggerSequenceLength": "1000", "Height": 1200, "555 AO-Block voltage": "false", "640 Shutter-Volts": "0.0000", "PixelType": "GRAY16", "445 Shutter-Block voltage": "false", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "520 Shutter-TriggerSequenceLength": "1000", "COM9-Name": "COM9", "Core-SLM": "", "555 AO-Volts": "0.0000", "555 Shutter-Block voltage": "false", "XYStage-Speed": "1800.0000", "520 AO-MaxVolts": "5.0000", "528 Shutter-SupportsTriggering": "0", "COM1-Description": "Serial port driver (boost:asio)", "PRIME-MetadataEnabled": "No", "520 Shutter-MinVolts": "0.0000", "470 Shutter-Sequenceable": "1", "PRIME-PreampDelay": "0", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-MinVolts": "0.0000", "ChannelIndex": 0, "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Core-AutoShutter": "1", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "Exposure-ms": 320, "PRIME-PP 2 ENABLED": "Yes", "640 Shutter-TriggerSampleRate": "100000", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "470 Shutter-IOChannel": "Dev1/ao10", "405 AO-Demo": "No", "PixelSizeUm": 1.1, "470 AO-Demo": "No", "470 Shutter-MaxVolts": "5.0000", "Vincent-D3-Name": "Vincent-D3", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FileName": "img_000000000_Il-G_000.tif", "LudlController-Reset": "Operate", "PRIME-Exposure": "320.00", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "445-Name": "DA Shutter", "555 Shutter-Name": "AnalogIO", "520 Shutter-TriggerSampleRate": "100000", "445 Shutter-IOChannel": "Dev1/ao9", "PRIME-TransposeCorrection": "0", "XYStage-StepSize": "0.1000", "445-Description": "DA device that is used as a shutter", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 AO-IOChannel": "Dev1/ao7", "520 Shutter-Name": "AnalogIO", "COM3-DataBits": "8", "640 AO-Volts": "0.0000", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "555 Shutter-IOChannel": "Dev1/ao13", "WaitInterval": null, "520 Shutter-Sequenceable": "1", "YPositionUm": 31506.2, "445-State": "0", "445-DA Device": "445 Shutter", "PRIME-CircularBufferAutoSize": "ON", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "555 AO-TriggerSampleRate": "100000", "445 Shutter-Volts": "0.0000", "XLIGHT Hub-Port": "COM3", "Core-AutoFocus": "", "COM4-Handshaking": "Off", "528 Shutter-MinVolts": "0.0000", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "445 Shutter-Name": "AnalogIO", "528 Shutter-Sequenceable": "1", "XYStage-TransposeMirrorX": "0", "XYStage-TransposeMirrorY": "0", "PRIME-ReadoutRate": "100MHz 16bit", "XYStage-Name": "Stage", "640 AO-SupportsTriggering": "0", "640-Name": "DA Shutter", "640 Shutter-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "640 AO-Block voltage": "false", "445 Shutter-Sequenceable": "1", "470 AO-MaxVolts": "5.0000", "COM4-BaudRate": "9600", "470-Description": "DA device that is used as a shutter", "520 Shutter-Description": "NI DAC", "PRIME-CCDTemperature": "-14.8500", "Time": "2019-12-18 21:36:42 -0500", "640 AO-TriggerSequenceLength": "1000", "470 AO-Name": "AnalogIO", "XLIGHT Disk slider-Label": "Disk Pos 40um", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "Binning": "1x1", "TE2000-ModelType": "1", "COM3-AnswerTimeout": "500.0000", "555 Shutter-SupportsTriggering": "0", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "TE2000-Port": "COM4", "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Description": "NI DAC", "COM4-AnswerTimeout": "500.0000", "COM1-DelayBetweenCharsMs": "0.0000", "FilterBlock-Label": "Confocal", "445 AO-Name": "AnalogIO", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-Timing-ReadoutTimeNs": "24672000.0000", "528 AO-Demo": "No", "520 AO-Sequenceable": "1", "555-Description": "DA device that is used as a shutter", "405-DA Device": "405 Shutter", "405 AO-TriggerSequenceLength": "1000", "640 AO-MinVolts": "0.0000", "640 Shutter-Block voltage": "false", "FrameIndex": 0, "PRIME-Port": "CMOS", "555 AO-MinVolts": "0.0000", "COM3-Description": "Serial port driver (boost:asio)", "528 AO-Description": "NI DAC", "555 AO-IOChannel": "Dev1/ao6", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "555 AO-TriggerInputLine": "/Dev1/PFI0", "PRIME-ActualInterval-ms": "390.5200", "LudlController-Port": "COM9", "445 Shutter-Demo": "No", "PRIME-Trigger-Expose Out-Mux": "1", "SliceIndex": 0, "PRIME-CCDTemperatureSetPoint": "-15.0000", "405 Shutter-MinVolts": "0.0000", "PRIME-ExposeOutMode": "First Row", "AxisPositions": null, "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Volts": "0.0000", "528-Name": "DA Shutter", "555-State": "0", "XLIGHT Dichroic-Label": "CFP/YFP", "445 AO-Sequenceable": "1", "405-Name": "DA Shutter", "405 AO-IOChannel": "Dev1/ao0", "Core-Camera": "PRIME", "Focus-Name": "Focus", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "405 Shutter-IOChannel": "Dev1/ao8", "640 AO-Demo": "No", "640 Shutter-SupportsTriggering": "0", "445 AO-Demo": "No", "555-DA Device": "555 Shutter", "470 Shutter-Description": "NI DAC", "528 AO-Volts": "0.0000", "COM1-DataBits": "8", "Vincent-D3-Address": "x", "520 AO-SupportsTriggering": "0", "405-Description": "DA device that is used as a shutter", "640 Shutter-MinVolts": "0.0000", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "405 AO-Name": "AnalogIO", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "640 AO-TriggerInputLine": "/Dev1/PFI0", "405 AO-TriggerInputLine": "/Dev1/PFI0", "640-Description": "DA device that is used as a shutter", "445 Shutter-TriggerSampleRate": "100000", "470 Shutter-TriggerSequenceLength": "1000", "COM1-StopBits": "1", "520 Shutter-SupportsTriggering": "0", "405-State": "0", "Core-Initialize": "1", "XLIGHT Emission Wheel-State": "3", "520 AO-Description": "NI DAC", "528 AO-TriggerInputLine": "/Dev1/PFI0", "445 Shutter-MaxVolts": "5.0000", "COM9-Handshaking": "Off", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "XLIGHT Spin motor-Label": "On (spinning)", "PRIME-PP 5 ENABLED": "No", "UUID": "9c124d35-00ea-451a-b7cd-1976544be3cc", "PRIME-TriggerMode": "Internal Trigger", "COM1-Handshaking": "Off", "PRIME-Timing-PostTriggerDelayNs": "49344000.0000", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "Focus-Position": "17741.5000", "528 Shutter-Block voltage": "false", "Channel": "Il-G", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "555 Shutter-Demo": "No", "405 Shutter-Demo": "No", "528 AO-Name": "AnalogIO", "520-State": "1", "Core-Galvo": "", "405 Shutter-TriggerSampleRate": "100000", "Focus-FocusStepSize_(nm)": "100", "PRIME-PixelType": "16bit", "COM4-DataBits": "8", "470 AO-IOChannel": "Dev1/ao2", "520 AO-MinVolts": "0.0000", "COM9-Parity": "None", "PRIME-CircularBufferEnabled": "ON", "PRIME-PP 6 Reset": "No", "640 AO-Description": "NI DAC", "Source": "PRIME", "445 AO-MinVolts": "0.0000", "PIEZO-Name": "Stage", "445 AO-Description": "NI DAC", "PRIME-CircularBufferFrameCount": "46", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "520 AO-Volts": "4.5000", "528 Shutter-TriggerSampleRate": "100000", "XPositionUm": 19731.9, "OpticalPath-State": "4", "PRIME-PP 3 THRESHOLD": "80", "PRIME-PVCAM Version": "3.7.1", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Gain": "1-HDR", "640 Shutter-TriggerSequenceLength": "1000", "528 AO-SupportsTriggering": "0", "445 AO-TriggerInputLine": "/Dev1/PFI0", "OpticalPath-Name": "OpticalPath", "PRIME-PP 4 ENABLED": "Yes", "PRIME-PVCAM Adapter Version": "1.3.29", "COM3-Name": "COM3", "405 AO-MaxVolts": "5.0000", "520 Shutter-Block voltage": "false", "PRIME-Timing-ClearingTimeNs": "49344000.0000", "Core-Focus": "PIEZO", "528 AO-Block voltage": "false", "TE2000-Description": "Nikon TE2000 hub", "528 AO-MinVolts": "0.0000", "COM4-Parity": "None", "Summary": { "Slices": 11, "ChContrastMin": [ 0, 0, 0, 0, 0, 0 ], "PixelAspect": 1, "SlicesFirst": true, "Interval_ms": 0, "Time": "2019-12-18 21:36:42 -0500", "Prefix": "seq_4", "InitialPositionList": [ { "Label": "Pos0", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.21], "Focus": [17741.5], "XYStage": [ 44542.200000000004, 31636.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos1", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.22], "Focus": [17741.4], "XYStage": [ 43801.100000000006, 31637.9 ] }, "GridColumnIndex": 0 }, { "Label": "Pos2", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.5], "XYStage": [ 38561.3, 32040.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos3", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.185], "Focus": [17741.3], "XYStage": [ 37698.4, 32041.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos4", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.16], "Focus": [17741.5], "XYStage": [ 32375.4, 31758.9 ] }, "GridColumnIndex": 0 }, { "Label": "Pos5", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.17000000000002], "Focus": [17741.3], "XYStage": [ 31770.7, 31758.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos6", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.4], "XYStage": [ 26536.800000000003, 31068.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos7", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.205], "Focus": [17741.4], "XYStage": [ 25420.2, 31068.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos8", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.85], "Focus": [17741.3], "XYStage": [ 21059, 31508 ] }, "GridColumnIndex": 0 }, { "Label": "Pos9", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.82], "Focus": [17741.3], "XYStage": [ 19731.9, 31506.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos10", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.79], "Focus": [17741.4], "XYStage": [ 14528.5, 32007.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos11", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.83], "Focus": [17741.2], "XYStage": [ 13830.300000000001, 32009.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos12", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.79], "Focus": [17741.3], "XYStage": [ 8775.300000000001, 32017.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos13", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.815], "Focus": [17741.3], "XYStage": [ 8109.200000000001, 32008.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos14", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.805], "Focus": [17741.3], "XYStage": [ 3483.3, 31908.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos15", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.82], "Focus": [17741.2], "XYStage": [ 2686.8, 31903.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos16", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [211.48000000000002], "Focus": [17741.1], "XYStage": [ -1978.9, 31973.600000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos17", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [211.51500000000001], "Focus": [17741.1], "XYStage": [ -2957.8, 31982.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos18", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.195], "Focus": [17741.5], "XYStage": [ 47747.4, -576.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos19", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.23000000000002], "Focus": [17741.6], "XYStage": [ 47053.9, -576.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos20", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.205], "Focus": [17741.4], "XYStage": [ 41871.200000000004, -479.8 ] }, "GridColumnIndex": 0 }, { "Label": "Pos21", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.4], "XYStage": [ 40882.600000000006, -392 ] }, "GridColumnIndex": 0 }, { "Label": "Pos22", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.24], "Focus": [17741.4], "XYStage": [ 35905.6, -135.3 ] }, "GridColumnIndex": 0 }, { "Label": "Pos23", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.195], "Focus": [17741.5], "XYStage": [ 34990.9, -132.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos24", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.46], "Focus": [17741.4], "XYStage": [ 29678.100000000002, -958.1 ] }, "GridColumnIndex": 0 }, { "Label": "Pos25", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.44], "Focus": [17741.5], "XYStage": [ 28983.600000000002, -939.4000000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos26", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.475], "Focus": [17741.5], "XYStage": [ 23019.7, -122 ] }, "GridColumnIndex": 0 }, { "Label": "Pos27", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.44], "Focus": [17741.7], "XYStage": [ 22428, -116.80000000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos28", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.46], "Focus": [17741.7], "XYStage": [ 16364.900000000001, -181.10000000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos29", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.48000000000002], "Focus": [17741.5], "XYStage": [ 15712.1, -180 ] }, "GridColumnIndex": 0 }, { "Label": "Pos30", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.45000000000002], "Focus": [17741.7], "XYStage": [ 10346.1, 77.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos31", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.49], "Focus": [17741.4], "XYStage": [ 9755.5, 85.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos32", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.475], "Focus": [17741.5], "XYStage": [ 4775.5, 293.90000000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos33", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.445], "Focus": [17741.4], "XYStage": [ 3954.9, 293.90000000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos34", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.455], "Focus": [17741.4], "XYStage": [ -1324.9, 545.6 ] }, "GridColumnIndex": 0 }, { "Label": "Pos35", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.445], "Focus": [17741.3], "XYStage": [ -2097, 576.6 ] }, "GridColumnIndex": 0 } ], "z-step_um": 10, "GridRow": 0, "BitDepth": 16, "Depth": 2, "Channels": 6, "Source": "Micro-Manager", "ChColors": [ -16711681, -256, -65281, -1, -1, -16776961 ], "MetadataVersion": 10, "KeepShutterOpenChannels": true, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536, 65536 ], "CustomIntervals_ms": [], "Height": 1200, "MicroManagerVersion": "1.4.23 20180219", "GridColumn": 0, "UUID": "14a13bf7-c944-4672-b873-fcfbbb7ea13d", "PixelType": "GRAY16", "Width": 1200, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\Yan\\20191218-YC113-S7S8-miseq", "Frames": 1, "Comment": "", "UserName": "Nikon", "PixelSize_um": 1.1, "ROI": [ 0, 0, 1200, 1200 ], "Date": "2019-12-18", "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC", "P-DAPI-high" ], "KeepShutterOpenSlices": true, "IJType": 1, "ComputerName": "ZadorConfocal2", "Positions": 36, "PositionIndex": 9, "CameraTimeout": 20000, "TimeFirst": false }, "640 AO-Name": "AnalogIO", "Vincent-D3-Command": "Close" }, "FrameKey-0-0-1": { "COM4-StopBits": "1", "528 AO-MaxVolts": "5.0000", "XLIGHT Excitation Wheel-State": "1", "470 Shutter-Name": "AnalogIO", "Core-Shutter": "520", "555 AO-Demo": "No", "XLIGHT Dichroic-State": "2", "XYStage-StepSize-Y": "0.1000", "ZPositionUm": 208.82, "XYStage-StepSize-X": "0.1000", "PRIME-FirmwareVersion": "20.28", "PRIME-ClearCycles": "2", "520-Name": "DA Shutter", "405 Shutter-Name": "AnalogIO", "PIEZO-StepSize": "0.0050", "PRIME-ClearMode": "Pre-Exposure", "470 AO-MinVolts": "0.0000", "405 AO-SupportsTriggering": "1", "CameraChannelIndex": 0, "XYStage-StartSpeed": "500.0000", "COM9-StopBits": "2", "405 AO-Description": "NI DAC", "COM4-DelayBetweenCharsMs": "0.0000", "445 AO-Block voltage": "false", "520 AO-TriggerInputLine": "/Dev1/PFI0", "555 AO-Description": "NI DAC", "520 AO-TriggerSampleRate": "100000", "COM4-Verbose": "1", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM4-Name": "COM4", "405 Shutter-Volts": "0.0000", "Vincent-D3-Port": "COM1", "PRIME-PP 4 MIN ADU AFFECTED": "200", "470 Shutter-MinVolts": "0.0000", "PRIME-PP 3 ENABLED": "Yes", "470 AO-Description": "NI DAC", "PRIME-Description": "PVCAM API device adapter", "520 Shutter-MaxVolts": "5.0000", "520 Shutter-Volts": "5.0000", "528 AO-Sequenceable": "1", "PRIME-Binning": "1x1", "555 Shutter-MaxVolts": "5.0000", "528 Shutter-TriggerSequenceLength": "1000", "405 Shutter-Block voltage": "false", "XLIGHT Spin motor-State": "1", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "Vincent-D3-Time to open (ms)": "35.0000", "Vincent-D3-Time to close (ms)": "35.0000", "445 AO-TriggerSampleRate": "100000", "640 Shutter-IOChannel": "Dev1/ao14", "555 AO-Sequenceable": "1", "520 AO-Name": "AnalogIO", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "470 AO-Block voltage": "false", "COM1-Verbose": "1", "COM9-AnswerTimeout": "10000.0000", "528 Shutter-Name": "AnalogIO", "PRIME-Trigger Timeout (secs)": "10", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "NextFrame": 0, "470 AO-TriggerSequenceLength": "1000", "PRIME-TransposeXY": "0", "445 AO-TriggerSequenceLength": "1000", "COM1-Name": "COM1", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "COM9-DataBits": "8", "COM1-AnswerTimeout": "500.0000", "Camera": "", "TE2000-Version": "V2.50-V2.50-V2.10", "528 Shutter-Volts": "0.0000", "XLIGHT Hub-Name": "XLIGHT Hub", "PIEZO-Description": "Ludl stage driver adapter", "PositionName": "Pos9", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSampleRate": "100000", "PRIME-FanSpeedSetpoint": "High", "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "405 AO-TriggerSampleRate": "100000", "470 Shutter-Block voltage": "false", "470-State": "0", "COM3-Parity": "None", "405 AO-Volts": "0.0000", "PRIME-TransposeMirrorY": "0", "PRIME-ChipName": "GS144BSI", "470 AO-Volts": "0.0000", "PRIME-TransposeMirrorX": "0", "445 Shutter-SupportsTriggering": "0", "470 Shutter-SupportsTriggering": "0", "XLIGHT Hub-Description": "XLIGHT hub", "PRIME-PreampOffLimit": "10000", "470 AO-Sequenceable": "1", "528 Shutter-MaxVolts": "5.0000", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "Core-XYStage": "XYStage", "470 AO-TriggerSampleRate": "100000", "PRIME-Timing-PreTriggerDelayNs": "24672000.0000", "528-DA Device": "528 Shutter", "Focus-Description": "Nikon TE2000 focus adapter", "FilterBlock-State": "5", "520 AO-Demo": "No", "COM3-DelayBetweenCharsMs": "0.0000", "470 Shutter-Volts": "0.0000", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "PRIME-PP 2 THRESHOLD": "125", "PRIME-PP 4 THRESHOLD": "75", "XYStage-Acceleration": "4.0000", "470 Shutter-Demo": "No", "PRIME-Name": "PMPCIECam00", "640-DA Device": "640 Shutter", "405 AO-Block voltage": "false", "ElapsedTime-ms": 374792, "PRIME-Offset": "100", "445 AO-IOChannel": "Dev1/ao1", "640 AO-MaxVolts": "5.0000", "PositionIndex": 9, "PRIME-PP 1 ENABLED": "Yes", "COM1-BaudRate": "9600", "640-State": "0", "COM3-Verbose": "1", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "LudlController-TransmissionDelay": "0", "PRIME-FullWellCapacity": "62000", "PRIME-SMARTStreamingEnabled": "No", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "640 Shutter-Description": "NI DAC", "528-Description": "DA device that is used as a shutter", "COM4-Description": "Serial port driver (boost:asio)", "405 Shutter-Description": "NI DAC", "TE2000-Name": "TE2000", "528 Shutter-Description": "NI DAC", "COM9-Description": "Serial port driver (boost:asio)", "LudlController-Name": "LudlController", "445 Shutter-TriggerSequenceLength": "1000", "PRIME-SerialNumber": "A18B203008", "445 AO-Volts": "0.0000", "PRIME-X-dimension": "1200", "PRIME-ShutterCloseDelay": "0", "XLIGHT Disk slider-State": "2", "Position": "Default", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 AO-IOChannel": "Dev1/ao5", "520 AO-IOChannel": "Dev1/ao4", "528 AO-TriggerSequenceLength": "1000", "405 Shutter-MaxVolts": "5.0000", "470-DA Device": "470 Shutter", "PRIME-FTCapable": "No", "Core-ChannelGroup": "Channels", "555 AO-SupportsTriggering": "0", "PRIME-Y-dimension": "1200", "Frame": 0, "555 Shutter-Sequenceable": "1", "PRIME-Timing-ExposureTimeNs": "499998000.0000", "Core-ImageProcessor": "", "528-State": "0", "520 AO-Block voltage": "false", "XLIGHT Excitation Wheel-Label": "CFP/YFP", "640 Shutter-Demo": "No", "COM3-StopBits": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "520 Shutter-IOChannel": "Dev1/ao11", "Width": 1200, "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-SupportsTriggering": "0", "640 AO-TriggerSampleRate": "100000", "520-Description": "DA device that is used as a shutter", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "405 Shutter-Sequenceable": "1", "405 Shutter-SupportsTriggering": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-TriggerSampleRate": "100000", "640 Shutter-MaxVolts": "5.0000", "555 AO-MaxVolts": "5.0000", "520 AO-TriggerSequenceLength": "1000", "COM3-BaudRate": "9600", "COM9-BaudRate": "9600", "Vincent-D3-Channel #": "Ch. #1", "PRIME-PMode": "Normal", "OpticalPath-Label": "Left(100)", "BitDepth": 16, "555 AO-TriggerSequenceLength": "1000", "445 Shutter-Description": "NI DAC", "405 AO-Sequenceable": "1", "PRIME-AcquisitionMethod": "Callbacks", "555 AO-Name": "AnalogIO", "PRIME-ShutterMode": "Pre-Exposure", "COM3-Handshaking": "Off", "445 AO-SupportsTriggering": "0", "ROI": "0-0-1200-1200", "COM9-Verbose": "1", "PRIME-Color": "OFF", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "PRIME-ShutterOpenDelay": "0", "COM1-Parity": "None", "Slice": 1, "SlicePosition": -40, "COM9-DelayBetweenCharsMs": "0.0000", "555-Name": "DA Shutter", "PIEZO-LudlSingleAxisName": "B", "XLIGHT Emission Wheel-Label": "565/24(G)", "640 Shutter-Name": "AnalogIO", "640 AO-Sequenceable": "1", "520 Shutter-Demo": "No", "528 Shutter-Demo": "No", "470-Name": "DA Shutter", "520-DA Device": "520 Shutter", "XYStage-Description": "Ludl XY stage driver adapter", "445 Shutter-MinVolts": "0.0000", "528 AO-TriggerSampleRate": "100000", "405 AO-MinVolts": "0.0000", "405 Shutter-TriggerSequenceLength": "1000", "Height": 1200, "555 AO-Block voltage": "false", "640 Shutter-Volts": "0.0000", "PixelType": "GRAY16", "445 Shutter-Block voltage": "false", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "520 Shutter-TriggerSequenceLength": "1000", "COM9-Name": "COM9", "Core-SLM": "", "555 AO-Volts": "0.0000", "555 Shutter-Block voltage": "false", "XYStage-Speed": "1800.0000", "520 AO-MaxVolts": "5.0000", "528 Shutter-SupportsTriggering": "0", "COM1-Description": "Serial port driver (boost:asio)", "PRIME-MetadataEnabled": "No", "520 Shutter-MinVolts": "0.0000", "470 Shutter-Sequenceable": "1", "PRIME-PreampDelay": "0", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-MinVolts": "0.0000", "ChannelIndex": 0, "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Core-AutoShutter": "1", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "Exposure-ms": 320, "PRIME-PP 2 ENABLED": "Yes", "640 Shutter-TriggerSampleRate": "100000", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "470 Shutter-IOChannel": "Dev1/ao10", "405 AO-Demo": "No", "PixelSizeUm": 1.1, "470 AO-Demo": "No", "470 Shutter-MaxVolts": "5.0000", "Vincent-D3-Name": "Vincent-D3", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FileName": "img_000000000_Il-G_001.tif", "LudlController-Reset": "Operate", "PRIME-Exposure": "320.00", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "445-Name": "DA Shutter", "555 Shutter-Name": "AnalogIO", "520 Shutter-TriggerSampleRate": "100000", "445 Shutter-IOChannel": "Dev1/ao9", "PRIME-TransposeCorrection": "0", "XYStage-StepSize": "0.1000", "445-Description": "DA device that is used as a shutter", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 AO-IOChannel": "Dev1/ao7", "520 Shutter-Name": "AnalogIO", "COM3-DataBits": "8", "640 AO-Volts": "0.0000", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "555 Shutter-IOChannel": "Dev1/ao13", "WaitInterval": null, "520 Shutter-Sequenceable": "1", "YPositionUm": 31506.2, "445-State": "0", "445-DA Device": "445 Shutter", "PRIME-CircularBufferAutoSize": "ON", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "555 AO-TriggerSampleRate": "100000", "445 Shutter-Volts": "0.0000", "XLIGHT Hub-Port": "COM3", "Core-AutoFocus": "", "COM4-Handshaking": "Off", "528 Shutter-MinVolts": "0.0000", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "445 Shutter-Name": "AnalogIO", "528 Shutter-Sequenceable": "1", "XYStage-TransposeMirrorX": "0", "XYStage-TransposeMirrorY": "0", "PRIME-ReadoutRate": "100MHz 16bit", "XYStage-Name": "Stage", "640 AO-SupportsTriggering": "0", "640-Name": "DA Shutter", "640 Shutter-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "640 AO-Block voltage": "false", "445 Shutter-Sequenceable": "1", "470 AO-MaxVolts": "5.0000", "COM4-BaudRate": "9600", "470-Description": "DA device that is used as a shutter", "520 Shutter-Description": "NI DAC", "PRIME-CCDTemperature": "-14.8500", "Time": "2019-12-18 21:36:42 -0500", "640 AO-TriggerSequenceLength": "1000", "470 AO-Name": "AnalogIO", "XLIGHT Disk slider-Label": "Disk Pos 40um", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "Binning": "1x1", "TE2000-ModelType": "1", "COM3-AnswerTimeout": "500.0000", "555 Shutter-SupportsTriggering": "0", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "TE2000-Port": "COM4", "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Description": "NI DAC", "COM4-AnswerTimeout": "500.0000", "COM1-DelayBetweenCharsMs": "0.0000", "FilterBlock-Label": "Confocal", "445 AO-Name": "AnalogIO", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-Timing-ReadoutTimeNs": "24672000.0000", "528 AO-Demo": "No", "520 AO-Sequenceable": "1", "555-Description": "DA device that is used as a shutter", "405-DA Device": "405 Shutter", "405 AO-TriggerSequenceLength": "1000", "640 AO-MinVolts": "0.0000", "640 Shutter-Block voltage": "false", "FrameIndex": 0, "PRIME-Port": "CMOS", "555 AO-MinVolts": "0.0000", "COM3-Description": "Serial port driver (boost:asio)", "528 AO-Description": "NI DAC", "555 AO-IOChannel": "Dev1/ao6", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "555 AO-TriggerInputLine": "/Dev1/PFI0", "PRIME-ActualInterval-ms": "390.5200", "LudlController-Port": "COM9", "445 Shutter-Demo": "No", "PRIME-Trigger-Expose Out-Mux": "1", "SliceIndex": 1, "PRIME-CCDTemperatureSetPoint": "-15.0000", "405 Shutter-MinVolts": "0.0000", "PRIME-ExposeOutMode": "First Row", "AxisPositions": null, "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Volts": "0.0000", "528-Name": "DA Shutter", "555-State": "0", "XLIGHT Dichroic-Label": "CFP/YFP", "445 AO-Sequenceable": "1", "405-Name": "DA Shutter", "405 AO-IOChannel": "Dev1/ao0", "Core-Camera": "PRIME", "Focus-Name": "Focus", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "405 Shutter-IOChannel": "Dev1/ao8", "640 AO-Demo": "No", "640 Shutter-SupportsTriggering": "0", "445 AO-Demo": "No", "555-DA Device": "555 Shutter", "470 Shutter-Description": "NI DAC", "528 AO-Volts": "0.0000", "COM1-DataBits": "8", "Vincent-D3-Address": "x", "520 AO-SupportsTriggering": "0", "405-Description": "DA device that is used as a shutter", "640 Shutter-MinVolts": "0.0000", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "405 AO-Name": "AnalogIO", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "640 AO-TriggerInputLine": "/Dev1/PFI0", "405 AO-TriggerInputLine": "/Dev1/PFI0", "640-Description": "DA device that is used as a shutter", "445 Shutter-TriggerSampleRate": "100000", "470 Shutter-TriggerSequenceLength": "1000", "COM1-StopBits": "1", "520 Shutter-SupportsTriggering": "0", "405-State": "0", "Core-Initialize": "1", "XLIGHT Emission Wheel-State": "3", "520 AO-Description": "NI DAC", "528 AO-TriggerInputLine": "/Dev1/PFI0", "445 Shutter-MaxVolts": "5.0000", "COM9-Handshaking": "Off", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "XLIGHT Spin motor-Label": "On (spinning)", "PRIME-PP 5 ENABLED": "No", "UUID": "292e45c4-d6c5-4715-a492-dff5c9bb6cc1", "PRIME-TriggerMode": "Internal Trigger", "COM1-Handshaking": "Off", "PRIME-Timing-PostTriggerDelayNs": "49344000.0000", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "Focus-Position": "17741.5000", "528 Shutter-Block voltage": "false", "Channel": "Il-G", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "555 Shutter-Demo": "No", "405 Shutter-Demo": "No", "528 AO-Name": "AnalogIO", "520-State": "1", "Core-Galvo": "", "405 Shutter-TriggerSampleRate": "100000", "Focus-FocusStepSize_(nm)": "100", "PRIME-PixelType": "16bit", "COM4-DataBits": "8", "470 AO-IOChannel": "Dev1/ao2", "520 AO-MinVolts": "0.0000", "COM9-Parity": "None", "PRIME-CircularBufferEnabled": "ON", "PRIME-PP 6 Reset": "No", "640 AO-Description": "NI DAC", "Source": "PRIME", "445 AO-MinVolts": "0.0000", "PIEZO-Name": "Stage", "445 AO-Description": "NI DAC", "PRIME-CircularBufferFrameCount": "46", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "520 AO-Volts": "4.5000", "528 Shutter-TriggerSampleRate": "100000", "XPositionUm": 19731.9, "OpticalPath-State": "4", "PRIME-PP 3 THRESHOLD": "80", "PRIME-PVCAM Version": "3.7.1", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Gain": "1-HDR", "640 Shutter-TriggerSequenceLength": "1000", "528 AO-SupportsTriggering": "0", "445 AO-TriggerInputLine": "/Dev1/PFI0", "OpticalPath-Name": "OpticalPath", "PRIME-PP 4 ENABLED": "Yes", "PRIME-PVCAM Adapter Version": "1.3.29", "COM3-Name": "COM3", "405 AO-MaxVolts": "5.0000", "520 Shutter-Block voltage": "false", "PRIME-Timing-ClearingTimeNs": "49344000.0000", "Core-Focus": "PIEZO", "528 AO-Block voltage": "false", "TE2000-Description": "Nikon TE2000 hub", "528 AO-MinVolts": "0.0000", "COM4-Parity": "None", "Summary": { "Slices": 11, "ChContrastMin": [ 0, 0, 0, 0, 0, 0 ], "PixelAspect": 1, "SlicesFirst": true, "Interval_ms": 0, "Time": "2019-12-18 21:36:42 -0500", "Prefix": "seq_4", "InitialPositionList": [ { "Label": "Pos0", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.21], "Focus": [17741.5], "XYStage": [ 44542.200000000004, 31636.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos1", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.22], "Focus": [17741.4], "XYStage": [ 43801.100000000006, 31637.9 ] }, "GridColumnIndex": 0 }, { "Label": "Pos2", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.5], "XYStage": [ 38561.3, 32040.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos3", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.185], "Focus": [17741.3], "XYStage": [ 37698.4, 32041.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos4", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.16], "Focus": [17741.5], "XYStage": [ 32375.4, 31758.9 ] }, "GridColumnIndex": 0 }, { "Label": "Pos5", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.17000000000002], "Focus": [17741.3], "XYStage": [ 31770.7, 31758.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos6", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.4], "XYStage": [ 26536.800000000003, 31068.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos7", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.205], "Focus": [17741.4], "XYStage": [ 25420.2, 31068.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos8", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.85], "Focus": [17741.3], "XYStage": [ 21059, 31508 ] }, "GridColumnIndex": 0 }, { "Label": "Pos9", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.82], "Focus": [17741.3], "XYStage": [ 19731.9, 31506.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos10", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.79], "Focus": [17741.4], "XYStage": [ 14528.5, 32007.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos11", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.83], "Focus": [17741.2], "XYStage": [ 13830.300000000001, 32009.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos12", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.79], "Focus": [17741.3], "XYStage": [ 8775.300000000001, 32017.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos13", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.815], "Focus": [17741.3], "XYStage": [ 8109.200000000001, 32008.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos14", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.805], "Focus": [17741.3], "XYStage": [ 3483.3, 31908.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos15", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.82], "Focus": [17741.2], "XYStage": [ 2686.8, 31903.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos16", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [211.48000000000002], "Focus": [17741.1], "XYStage": [ -1978.9, 31973.600000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos17", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [211.51500000000001], "Focus": [17741.1], "XYStage": [ -2957.8, 31982.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos18", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.195], "Focus": [17741.5], "XYStage": [ 47747.4, -576.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos19", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.23000000000002], "Focus": [17741.6], "XYStage": [ 47053.9, -576.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos20", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.205], "Focus": [17741.4], "XYStage": [ 41871.200000000004, -479.8 ] }, "GridColumnIndex": 0 }, { "Label": "Pos21", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.4], "XYStage": [ 40882.600000000006, -392 ] }, "GridColumnIndex": 0 }, { "Label": "Pos22", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.24], "Focus": [17741.4], "XYStage": [ 35905.6, -135.3 ] }, "GridColumnIndex": 0 }, { "Label": "Pos23", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.195], "Focus": [17741.5], "XYStage": [ 34990.9, -132.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos24", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.46], "Focus": [17741.4], "XYStage": [ 29678.100000000002, -958.1 ] }, "GridColumnIndex": 0 }, { "Label": "Pos25", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.44], "Focus": [17741.5], "XYStage": [ 28983.600000000002, -939.4000000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos26", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.475], "Focus": [17741.5], "XYStage": [ 23019.7, -122 ] }, "GridColumnIndex": 0 }, { "Label": "Pos27", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.44], "Focus": [17741.7], "XYStage": [ 22428, -116.80000000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos28", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.46], "Focus": [17741.7], "XYStage": [ 16364.900000000001, -181.10000000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos29", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.48000000000002], "Focus": [17741.5], "XYStage": [ 15712.1, -180 ] }, "GridColumnIndex": 0 }, { "Label": "Pos30", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.45000000000002], "Focus": [17741.7], "XYStage": [ 10346.1, 77.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos31", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.49], "Focus": [17741.4], "XYStage": [ 9755.5, 85.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos32", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.475], "Focus": [17741.5], "XYStage": [ 4775.5, 293.90000000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos33", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.445], "Focus": [17741.4], "XYStage": [ 3954.9, 293.90000000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos34", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.455], "Focus": [17741.4], "XYStage": [ -1324.9, 545.6 ] }, "GridColumnIndex": 0 }, { "Label": "Pos35", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.445], "Focus": [17741.3], "XYStage": [ -2097, 576.6 ] }, "GridColumnIndex": 0 } ], "z-step_um": 10, "GridRow": 0, "BitDepth": 16, "Depth": 2, "Channels": 6, "Source": "Micro-Manager", "ChColors": [ -16711681, -256, -65281, -1, -1, -16776961 ], "MetadataVersion": 10, "KeepShutterOpenChannels": true, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536, 65536 ], "CustomIntervals_ms": [], "Height": 1200, "MicroManagerVersion": "1.4.23 20180219", "GridColumn": 0, "UUID": "14a13bf7-c944-4672-b873-fcfbbb7ea13d", "PixelType": "GRAY16", "Width": 1200, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\Yan\\20191218-YC113-S7S8-miseq", "Frames": 1, "Comment": "", "UserName": "Nikon", "PixelSize_um": 1.1, "ROI": [ 0, 0, 1200, 1200 ], "Date": "2019-12-18", "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC", "P-DAPI-high" ], "KeepShutterOpenSlices": true, "IJType": 1, "ComputerName": "ZadorConfocal2", "Positions": 36, "PositionIndex": 9, "CameraTimeout": 20000, "TimeFirst": false }, "640 AO-Name": "AnalogIO", "Vincent-D3-Command": "Close" }, "FrameKey-0-0-2": { "COM4-StopBits": "1", "528 AO-MaxVolts": "5.0000", "XLIGHT Excitation Wheel-State": "1", "470 Shutter-Name": "AnalogIO", "Core-Shutter": "520", "555 AO-Demo": "No", "XLIGHT Dichroic-State": "2", "XYStage-StepSize-Y": "0.1000", "ZPositionUm": 218.82, "XYStage-StepSize-X": "0.1000", "PRIME-FirmwareVersion": "20.28", "PRIME-ClearCycles": "2", "520-Name": "DA Shutter", "405 Shutter-Name": "AnalogIO", "PIEZO-StepSize": "0.0050", "PRIME-ClearMode": "Pre-Exposure", "470 AO-MinVolts": "0.0000", "405 AO-SupportsTriggering": "1", "CameraChannelIndex": 0, "XYStage-StartSpeed": "500.0000", "COM9-StopBits": "2", "405 AO-Description": "NI DAC", "COM4-DelayBetweenCharsMs": "0.0000", "445 AO-Block voltage": "false", "520 AO-TriggerInputLine": "/Dev1/PFI0", "555 AO-Description": "NI DAC", "520 AO-TriggerSampleRate": "100000", "COM4-Verbose": "1", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM4-Name": "COM4", "405 Shutter-Volts": "0.0000", "Vincent-D3-Port": "COM1", "PRIME-PP 4 MIN ADU AFFECTED": "200", "470 Shutter-MinVolts": "0.0000", "PRIME-PP 3 ENABLED": "Yes", "470 AO-Description": "NI DAC", "PRIME-Description": "PVCAM API device adapter", "520 Shutter-MaxVolts": "5.0000", "520 Shutter-Volts": "5.0000", "528 AO-Sequenceable": "1", "PRIME-Binning": "1x1", "555 Shutter-MaxVolts": "5.0000", "528 Shutter-TriggerSequenceLength": "1000", "405 Shutter-Block voltage": "false", "XLIGHT Spin motor-State": "1", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "Vincent-D3-Time to open (ms)": "35.0000", "Vincent-D3-Time to close (ms)": "35.0000", "445 AO-TriggerSampleRate": "100000", "640 Shutter-IOChannel": "Dev1/ao14", "555 AO-Sequenceable": "1", "520 AO-Name": "AnalogIO", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "470 AO-Block voltage": "false", "COM1-Verbose": "1", "COM9-AnswerTimeout": "10000.0000", "528 Shutter-Name": "AnalogIO", "PRIME-Trigger Timeout (secs)": "10", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "NextFrame": 0, "470 AO-TriggerSequenceLength": "1000", "PRIME-TransposeXY": "0", "445 AO-TriggerSequenceLength": "1000", "COM1-Name": "COM1", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "COM9-DataBits": "8", "COM1-AnswerTimeout": "500.0000", "Camera": "", "TE2000-Version": "V2.50-V2.50-V2.10", "528 Shutter-Volts": "0.0000", "XLIGHT Hub-Name": "XLIGHT Hub", "PIEZO-Description": "Ludl stage driver adapter", "PositionName": "Pos9", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSampleRate": "100000", "PRIME-FanSpeedSetpoint": "High", "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "405 AO-TriggerSampleRate": "100000", "470 Shutter-Block voltage": "false", "470-State": "0", "COM3-Parity": "None", "405 AO-Volts": "0.0000", "PRIME-TransposeMirrorY": "0", "PRIME-ChipName": "GS144BSI", "470 AO-Volts": "0.0000", "PRIME-TransposeMirrorX": "0", "445 Shutter-SupportsTriggering": "0", "470 Shutter-SupportsTriggering": "0", "XLIGHT Hub-Description": "XLIGHT hub", "PRIME-PreampOffLimit": "10000", "470 AO-Sequenceable": "1", "528 Shutter-MaxVolts": "5.0000", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "Core-XYStage": "XYStage", "470 AO-TriggerSampleRate": "100000", "PRIME-Timing-PreTriggerDelayNs": "24672000.0000", "528-DA Device": "528 Shutter", "Focus-Description": "Nikon TE2000 focus adapter", "FilterBlock-State": "5", "520 AO-Demo": "No", "COM3-DelayBetweenCharsMs": "0.0000", "470 Shutter-Volts": "0.0000", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "PRIME-PP 2 THRESHOLD": "125", "PRIME-PP 4 THRESHOLD": "75", "XYStage-Acceleration": "4.0000", "470 Shutter-Demo": "No", "PRIME-Name": "PMPCIECam00", "640-DA Device": "640 Shutter", "405 AO-Block voltage": "false", "ElapsedTime-ms": 375404, "PRIME-Offset": "100", "445 AO-IOChannel": "Dev1/ao1", "640 AO-MaxVolts": "5.0000", "PositionIndex": 9, "PRIME-PP 1 ENABLED": "Yes", "COM1-BaudRate": "9600", "640-State": "0", "COM3-Verbose": "1", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "LudlController-TransmissionDelay": "0", "PRIME-FullWellCapacity": "62000", "PRIME-SMARTStreamingEnabled": "No", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "640 Shutter-Description": "NI DAC", "528-Description": "DA device that is used as a shutter", "COM4-Description": "Serial port driver (boost:asio)", "405 Shutter-Description": "NI DAC", "TE2000-Name": "TE2000", "528 Shutter-Description": "NI DAC", "COM9-Description": "Serial port driver (boost:asio)", "LudlController-Name": "LudlController", "445 Shutter-TriggerSequenceLength": "1000", "PRIME-SerialNumber": "A18B203008", "445 AO-Volts": "0.0000", "PRIME-X-dimension": "1200", "PRIME-ShutterCloseDelay": "0", "XLIGHT Disk slider-State": "2", "Position": "Default", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 AO-IOChannel": "Dev1/ao5", "520 AO-IOChannel": "Dev1/ao4", "528 AO-TriggerSequenceLength": "1000", "405 Shutter-MaxVolts": "5.0000", "470-DA Device": "470 Shutter", "PRIME-FTCapable": "No", "Core-ChannelGroup": "Channels", "555 AO-SupportsTriggering": "0", "PRIME-Y-dimension": "1200", "Frame": 0, "555 Shutter-Sequenceable": "1", "PRIME-Timing-ExposureTimeNs": "499998000.0000", "Core-ImageProcessor": "", "528-State": "0", "520 AO-Block voltage": "false", "XLIGHT Excitation Wheel-Label": "CFP/YFP", "640 Shutter-Demo": "No", "COM3-StopBits": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "520 Shutter-IOChannel": "Dev1/ao11", "Width": 1200, "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-SupportsTriggering": "0", "640 AO-TriggerSampleRate": "100000", "520-Description": "DA device that is used as a shutter", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "405 Shutter-Sequenceable": "1", "405 Shutter-SupportsTriggering": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-TriggerSampleRate": "100000", "640 Shutter-MaxVolts": "5.0000", "555 AO-MaxVolts": "5.0000", "520 AO-TriggerSequenceLength": "1000", "COM3-BaudRate": "9600", "COM9-BaudRate": "9600", "Vincent-D3-Channel #": "Ch. #1", "PRIME-PMode": "Normal", "OpticalPath-Label": "Left(100)", "BitDepth": 16, "555 AO-TriggerSequenceLength": "1000", "445 Shutter-Description": "NI DAC", "405 AO-Sequenceable": "1", "PRIME-AcquisitionMethod": "Callbacks", "555 AO-Name": "AnalogIO", "PRIME-ShutterMode": "Pre-Exposure", "COM3-Handshaking": "Off", "445 AO-SupportsTriggering": "0", "ROI": "0-0-1200-1200", "COM9-Verbose": "1", "PRIME-Color": "OFF", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "PRIME-ShutterOpenDelay": "0", "COM1-Parity": "None", "Slice": 2, "SlicePosition": -30, "COM9-DelayBetweenCharsMs": "0.0000", "555-Name": "DA Shutter", "PIEZO-LudlSingleAxisName": "B", "XLIGHT Emission Wheel-Label": "565/24(G)", "640 Shutter-Name": "AnalogIO", "640 AO-Sequenceable": "1", "520 Shutter-Demo": "No", "528 Shutter-Demo": "No", "470-Name": "DA Shutter", "520-DA Device": "520 Shutter", "XYStage-Description": "Ludl XY stage driver adapter", "445 Shutter-MinVolts": "0.0000", "528 AO-TriggerSampleRate": "100000", "405 AO-MinVolts": "0.0000", "405 Shutter-TriggerSequenceLength": "1000", "Height": 1200, "555 AO-Block voltage": "false", "640 Shutter-Volts": "0.0000", "PixelType": "GRAY16", "445 Shutter-Block voltage": "false", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "520 Shutter-TriggerSequenceLength": "1000", "COM9-Name": "COM9", "Core-SLM": "", "555 AO-Volts": "0.0000", "555 Shutter-Block voltage": "false", "XYStage-Speed": "1800.0000", "520 AO-MaxVolts": "5.0000", "528 Shutter-SupportsTriggering": "0", "COM1-Description": "Serial port driver (boost:asio)", "PRIME-MetadataEnabled": "No", "520 Shutter-MinVolts": "0.0000", "470 Shutter-Sequenceable": "1", "PRIME-PreampDelay": "0", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-MinVolts": "0.0000", "ChannelIndex": 0, "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Core-AutoShutter": "1", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "Exposure-ms": 320, "PRIME-PP 2 ENABLED": "Yes", "640 Shutter-TriggerSampleRate": "100000", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "470 Shutter-IOChannel": "Dev1/ao10", "405 AO-Demo": "No", "PixelSizeUm": 1.1, "470 AO-Demo": "No", "470 Shutter-MaxVolts": "5.0000", "Vincent-D3-Name": "Vincent-D3", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FileName": "img_000000000_Il-G_002.tif", "LudlController-Reset": "Operate", "PRIME-Exposure": "320.00", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "445-Name": "DA Shutter", "555 Shutter-Name": "AnalogIO", "520 Shutter-TriggerSampleRate": "100000", "445 Shutter-IOChannel": "Dev1/ao9", "PRIME-TransposeCorrection": "0", "XYStage-StepSize": "0.1000", "445-Description": "DA device that is used as a shutter", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 AO-IOChannel": "Dev1/ao7", "520 Shutter-Name": "AnalogIO", "COM3-DataBits": "8", "640 AO-Volts": "0.0000", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "555 Shutter-IOChannel": "Dev1/ao13", "WaitInterval": null, "520 Shutter-Sequenceable": "1", "YPositionUm": 31506.2, "445-State": "0", "445-DA Device": "445 Shutter", "PRIME-CircularBufferAutoSize": "ON", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "555 AO-TriggerSampleRate": "100000", "445 Shutter-Volts": "0.0000", "XLIGHT Hub-Port": "COM3", "Core-AutoFocus": "", "COM4-Handshaking": "Off", "528 Shutter-MinVolts": "0.0000", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "445 Shutter-Name": "AnalogIO", "528 Shutter-Sequenceable": "1", "XYStage-TransposeMirrorX": "0", "XYStage-TransposeMirrorY": "0", "PRIME-ReadoutRate": "100MHz 16bit", "XYStage-Name": "Stage", "640 AO-SupportsTriggering": "0", "640-Name": "DA Shutter", "640 Shutter-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "640 AO-Block voltage": "false", "445 Shutter-Sequenceable": "1", "470 AO-MaxVolts": "5.0000", "COM4-BaudRate": "9600", "470-Description": "DA device that is used as a shutter", "520 Shutter-Description": "NI DAC", "PRIME-CCDTemperature": "-14.8500", "Time": "2019-12-18 21:36:43 -0500", "640 AO-TriggerSequenceLength": "1000", "470 AO-Name": "AnalogIO", "XLIGHT Disk slider-Label": "Disk Pos 40um", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "Binning": "1x1", "TE2000-ModelType": "1", "COM3-AnswerTimeout": "500.0000", "555 Shutter-SupportsTriggering": "0", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "TE2000-Port": "COM4", "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Description": "NI DAC", "COM4-AnswerTimeout": "500.0000", "COM1-DelayBetweenCharsMs": "0.0000", "FilterBlock-Label": "Confocal", "445 AO-Name": "AnalogIO", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-Timing-ReadoutTimeNs": "24672000.0000", "528 AO-Demo": "No", "520 AO-Sequenceable": "1", "555-Description": "DA device that is used as a shutter", "405-DA Device": "405 Shutter", "405 AO-TriggerSequenceLength": "1000", "640 AO-MinVolts": "0.0000", "640 Shutter-Block voltage": "false", "FrameIndex": 0, "PRIME-Port": "CMOS", "555 AO-MinVolts": "0.0000", "COM3-Description": "Serial port driver (boost:asio)", "528 AO-Description": "NI DAC", "555 AO-IOChannel": "Dev1/ao6", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "555 AO-TriggerInputLine": "/Dev1/PFI0", "PRIME-ActualInterval-ms": "390.5200", "LudlController-Port": "COM9", "445 Shutter-Demo": "No", "PRIME-Trigger-Expose Out-Mux": "1", "SliceIndex": 2, "PRIME-CCDTemperatureSetPoint": "-15.0000", "405 Shutter-MinVolts": "0.0000", "PRIME-ExposeOutMode": "First Row", "AxisPositions": null, "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Volts": "0.0000", "528-Name": "DA Shutter", "555-State": "0", "XLIGHT Dichroic-Label": "CFP/YFP", "445 AO-Sequenceable": "1", "405-Name": "DA Shutter", "405 AO-IOChannel": "Dev1/ao0", "Core-Camera": "PRIME", "Focus-Name": "Focus", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "405 Shutter-IOChannel": "Dev1/ao8", "640 AO-Demo": "No", "640 Shutter-SupportsTriggering": "0", "445 AO-Demo": "No", "555-DA Device": "555 Shutter", "470 Shutter-Description": "NI DAC", "528 AO-Volts": "0.0000", "COM1-DataBits": "8", "Vincent-D3-Address": "x", "520 AO-SupportsTriggering": "0", "405-Description": "DA device that is used as a shutter", "640 Shutter-MinVolts": "0.0000", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "405 AO-Name": "AnalogIO", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "640 AO-TriggerInputLine": "/Dev1/PFI0", "405 AO-TriggerInputLine": "/Dev1/PFI0", "640-Description": "DA device that is used as a shutter", "445 Shutter-TriggerSampleRate": "100000", "470 Shutter-TriggerSequenceLength": "1000", "COM1-StopBits": "1", "520 Shutter-SupportsTriggering": "0", "405-State": "0", "Core-Initialize": "1", "XLIGHT Emission Wheel-State": "3", "520 AO-Description": "NI DAC", "528 AO-TriggerInputLine": "/Dev1/PFI0", "445 Shutter-MaxVolts": "5.0000", "COM9-Handshaking": "Off", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "XLIGHT Spin motor-Label": "On (spinning)", "PRIME-PP 5 ENABLED": "No", "UUID": "0f55de71-e769-4cd4-a56d-16969d390a34", "PRIME-TriggerMode": "Internal Trigger", "COM1-Handshaking": "Off", "PRIME-Timing-PostTriggerDelayNs": "49344000.0000", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "Focus-Position": "17741.5000", "528 Shutter-Block voltage": "false", "Channel": "Il-G", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "555 Shutter-Demo": "No", "405 Shutter-Demo": "No", "528 AO-Name": "AnalogIO", "520-State": "1", "Core-Galvo": "", "405 Shutter-TriggerSampleRate": "100000", "Focus-FocusStepSize_(nm)": "100", "PRIME-PixelType": "16bit", "COM4-DataBits": "8", "470 AO-IOChannel": "Dev1/ao2", "520 AO-MinVolts": "0.0000", "COM9-Parity": "None", "PRIME-CircularBufferEnabled": "ON", "PRIME-PP 6 Reset": "No", "640 AO-Description": "NI DAC", "Source": "PRIME", "445 AO-MinVolts": "0.0000", "PIEZO-Name": "Stage", "445 AO-Description": "NI DAC", "PRIME-CircularBufferFrameCount": "46", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "520 AO-Volts": "4.5000", "528 Shutter-TriggerSampleRate": "100000", "XPositionUm": 19731.9, "OpticalPath-State": "4", "PRIME-PP 3 THRESHOLD": "80", "PRIME-PVCAM Version": "3.7.1", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Gain": "1-HDR", "640 Shutter-TriggerSequenceLength": "1000", "528 AO-SupportsTriggering": "0", "445 AO-TriggerInputLine": "/Dev1/PFI0", "OpticalPath-Name": "OpticalPath", "PRIME-PP 4 ENABLED": "Yes", "PRIME-PVCAM Adapter Version": "1.3.29", "COM3-Name": "COM3", "405 AO-MaxVolts": "5.0000", "520 Shutter-Block voltage": "false", "PRIME-Timing-ClearingTimeNs": "49344000.0000", "Core-Focus": "PIEZO", "528 AO-Block voltage": "false", "TE2000-Description": "Nikon TE2000 hub", "528 AO-MinVolts": "0.0000", "COM4-Parity": "None", "Summary": { "Slices": 11, "ChContrastMin": [ 0, 0, 0, 0, 0, 0 ], "PixelAspect": 1, "SlicesFirst": true, "Interval_ms": 0, "Time": "2019-12-18 21:36:42 -0500", "Prefix": "seq_4", "InitialPositionList": [ { "Label": "Pos0", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.21], "Focus": [17741.5], "XYStage": [ 44542.200000000004, 31636.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos1", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.22], "Focus": [17741.4], "XYStage": [ 43801.100000000006, 31637.9 ] }, "GridColumnIndex": 0 }, { "Label": "Pos2", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.5], "XYStage": [ 38561.3, 32040.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos3", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.185], "Focus": [17741.3], "XYStage": [ 37698.4, 32041.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos4", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.16], "Focus": [17741.5], "XYStage": [ 32375.4, 31758.9 ] }, "GridColumnIndex": 0 }, { "Label": "Pos5", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.17000000000002], "Focus": [17741.3], "XYStage": [ 31770.7, 31758.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos6", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.4], "XYStage": [ 26536.800000000003, 31068.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos7", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.205], "Focus": [17741.4], "XYStage": [ 25420.2, 31068.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos8", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.85], "Focus": [17741.3], "XYStage": [ 21059, 31508 ] }, "GridColumnIndex": 0 }, { "Label": "Pos9", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.82], "Focus": [17741.3], "XYStage": [ 19731.9, 31506.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos10", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.79], "Focus": [17741.4], "XYStage": [ 14528.5, 32007.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos11", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.83], "Focus": [17741.2], "XYStage": [ 13830.300000000001, 32009.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos12", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.79], "Focus": [17741.3], "XYStage": [ 8775.300000000001, 32017.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos13", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.815], "Focus": [17741.3], "XYStage": [ 8109.200000000001, 32008.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos14", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.805], "Focus": [17741.3], "XYStage": [ 3483.3, 31908.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos15", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.82], "Focus": [17741.2], "XYStage": [ 2686.8, 31903.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos16", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [211.48000000000002], "Focus": [17741.1], "XYStage": [ -1978.9, 31973.600000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos17", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [211.51500000000001], "Focus": [17741.1], "XYStage": [ -2957.8, 31982.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos18", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.195], "Focus": [17741.5], "XYStage": [ 47747.4, -576.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos19", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.23000000000002], "Focus": [17741.6], "XYStage": [ 47053.9, -576.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos20", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.205], "Focus": [17741.4], "XYStage": [ 41871.200000000004, -479.8 ] }, "GridColumnIndex": 0 }, { "Label": "Pos21", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.4], "XYStage": [ 40882.600000000006, -392 ] }, "GridColumnIndex": 0 }, { "Label": "Pos22", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.24], "Focus": [17741.4], "XYStage": [ 35905.6, -135.3 ] }, "GridColumnIndex": 0 }, { "Label": "Pos23", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.195], "Focus": [17741.5], "XYStage": [ 34990.9, -132.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos24", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.46], "Focus": [17741.4], "XYStage": [ 29678.100000000002, -958.1 ] }, "GridColumnIndex": 0 }, { "Label": "Pos25", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.44], "Focus": [17741.5], "XYStage": [ 28983.600000000002, -939.4000000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos26", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.475], "Focus": [17741.5], "XYStage": [ 23019.7, -122 ] }, "GridColumnIndex": 0 }, { "Label": "Pos27", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.44], "Focus": [17741.7], "XYStage": [ 22428, -116.80000000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos28", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.46], "Focus": [17741.7], "XYStage": [ 16364.900000000001, -181.10000000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos29", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.48000000000002], "Focus": [17741.5], "XYStage": [ 15712.1, -180 ] }, "GridColumnIndex": 0 }, { "Label": "Pos30", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.45000000000002], "Focus": [17741.7], "XYStage": [ 10346.1, 77.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos31", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.49], "Focus": [17741.4], "XYStage": [ 9755.5, 85.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos32", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.475], "Focus": [17741.5], "XYStage": [ 4775.5, 293.90000000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos33", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.445], "Focus": [17741.4], "XYStage": [ 3954.9, 293.90000000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos34", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.455], "Focus": [17741.4], "XYStage": [ -1324.9, 545.6 ] }, "GridColumnIndex": 0 }, { "Label": "Pos35", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.445], "Focus": [17741.3], "XYStage": [ -2097, 576.6 ] }, "GridColumnIndex": 0 } ], "z-step_um": 10, "GridRow": 0, "BitDepth": 16, "Depth": 2, "Channels": 6, "Source": "Micro-Manager", "ChColors": [ -16711681, -256, -65281, -1, -1, -16776961 ], "MetadataVersion": 10, "KeepShutterOpenChannels": true, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536, 65536 ], "CustomIntervals_ms": [], "Height": 1200, "MicroManagerVersion": "1.4.23 20180219", "GridColumn": 0, "UUID": "14a13bf7-c944-4672-b873-fcfbbb7ea13d", "PixelType": "GRAY16", "Width": 1200, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\Yan\\20191218-YC113-S7S8-miseq", "Frames": 1, "Comment": "", "UserName": "Nikon", "PixelSize_um": 1.1, "ROI": [ 0, 0, 1200, 1200 ], "Date": "2019-12-18", "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC", "P-DAPI-high" ], "KeepShutterOpenSlices": true, "IJType": 1, "ComputerName": "ZadorConfocal2", "Positions": 36, "PositionIndex": 9, "CameraTimeout": 20000, "TimeFirst": false }, "640 AO-Name": "AnalogIO", "Vincent-D3-Command": "Close" }, "FrameKey-0-0-3": { "COM4-StopBits": "1", "528 AO-MaxVolts": "5.0000", "XLIGHT Excitation Wheel-State": "1", "470 Shutter-Name": "AnalogIO", "Core-Shutter": "520", "555 AO-Demo": "No", "XLIGHT Dichroic-State": "2", "XYStage-StepSize-Y": "0.1000", "ZPositionUm": 228.82, "XYStage-StepSize-X": "0.1000", "PRIME-FirmwareVersion": "20.28", "PRIME-ClearCycles": "2", "520-Name": "DA Shutter", "405 Shutter-Name": "AnalogIO", "PIEZO-StepSize": "0.0050", "PRIME-ClearMode": "Pre-Exposure", "470 AO-MinVolts": "0.0000", "405 AO-SupportsTriggering": "1", "CameraChannelIndex": 0, "XYStage-StartSpeed": "500.0000", "COM9-StopBits": "2", "405 AO-Description": "NI DAC", "COM4-DelayBetweenCharsMs": "0.0000", "445 AO-Block voltage": "false", "520 AO-TriggerInputLine": "/Dev1/PFI0", "555 AO-Description": "NI DAC", "520 AO-TriggerSampleRate": "100000", "COM4-Verbose": "1", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM4-Name": "COM4", "405 Shutter-Volts": "0.0000", "Vincent-D3-Port": "COM1", "PRIME-PP 4 MIN ADU AFFECTED": "200", "470 Shutter-MinVolts": "0.0000", "PRIME-PP 3 ENABLED": "Yes", "470 AO-Description": "NI DAC", "PRIME-Description": "PVCAM API device adapter", "520 Shutter-MaxVolts": "5.0000", "520 Shutter-Volts": "5.0000", "528 AO-Sequenceable": "1", "PRIME-Binning": "1x1", "555 Shutter-MaxVolts": "5.0000", "528 Shutter-TriggerSequenceLength": "1000", "405 Shutter-Block voltage": "false", "XLIGHT Spin motor-State": "1", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "Vincent-D3-Time to open (ms)": "35.0000", "Vincent-D3-Time to close (ms)": "35.0000", "445 AO-TriggerSampleRate": "100000", "640 Shutter-IOChannel": "Dev1/ao14", "555 AO-Sequenceable": "1", "520 AO-Name": "AnalogIO", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "470 AO-Block voltage": "false", "COM1-Verbose": "1", "COM9-AnswerTimeout": "10000.0000", "528 Shutter-Name": "AnalogIO", "PRIME-Trigger Timeout (secs)": "10", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "NextFrame": 0, "470 AO-TriggerSequenceLength": "1000", "PRIME-TransposeXY": "0", "445 AO-TriggerSequenceLength": "1000", "COM1-Name": "COM1", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "COM9-DataBits": "8", "COM1-AnswerTimeout": "500.0000", "Camera": "", "TE2000-Version": "V2.50-V2.50-V2.10", "528 Shutter-Volts": "0.0000", "XLIGHT Hub-Name": "XLIGHT Hub", "PIEZO-Description": "Ludl stage driver adapter", "PositionName": "Pos9", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSampleRate": "100000", "PRIME-FanSpeedSetpoint": "High", "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "405 AO-TriggerSampleRate": "100000", "470 Shutter-Block voltage": "false", "470-State": "0", "COM3-Parity": "None", "405 AO-Volts": "0.0000", "PRIME-TransposeMirrorY": "0", "PRIME-ChipName": "GS144BSI", "470 AO-Volts": "0.0000", "PRIME-TransposeMirrorX": "0", "445 Shutter-SupportsTriggering": "0", "470 Shutter-SupportsTriggering": "0", "XLIGHT Hub-Description": "XLIGHT hub", "PRIME-PreampOffLimit": "10000", "470 AO-Sequenceable": "1", "528 Shutter-MaxVolts": "5.0000", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "Core-XYStage": "XYStage", "470 AO-TriggerSampleRate": "100000", "PRIME-Timing-PreTriggerDelayNs": "24672000.0000", "528-DA Device": "528 Shutter", "Focus-Description": "Nikon TE2000 focus adapter", "FilterBlock-State": "5", "520 AO-Demo": "No", "COM3-DelayBetweenCharsMs": "0.0000", "470 Shutter-Volts": "0.0000", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "PRIME-PP 2 THRESHOLD": "125", "PRIME-PP 4 THRESHOLD": "75", "XYStage-Acceleration": "4.0000", "470 Shutter-Demo": "No", "PRIME-Name": "PMPCIECam00", "640-DA Device": "640 Shutter", "405 AO-Block voltage": "false", "ElapsedTime-ms": 376017, "PRIME-Offset": "100", "445 AO-IOChannel": "Dev1/ao1", "640 AO-MaxVolts": "5.0000", "PositionIndex": 9, "PRIME-PP 1 ENABLED": "Yes", "COM1-BaudRate": "9600", "640-State": "0", "COM3-Verbose": "1", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "LudlController-TransmissionDelay": "0", "PRIME-FullWellCapacity": "62000", "PRIME-SMARTStreamingEnabled": "No", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "640 Shutter-Description": "NI DAC", "528-Description": "DA device that is used as a shutter", "COM4-Description": "Serial port driver (boost:asio)", "405 Shutter-Description": "NI DAC", "TE2000-Name": "TE2000", "528 Shutter-Description": "NI DAC", "COM9-Description": "Serial port driver (boost:asio)", "LudlController-Name": "LudlController", "445 Shutter-TriggerSequenceLength": "1000", "PRIME-SerialNumber": "A18B203008", "445 AO-Volts": "0.0000", "PRIME-X-dimension": "1200", "PRIME-ShutterCloseDelay": "0", "XLIGHT Disk slider-State": "2", "Position": "Default", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 AO-IOChannel": "Dev1/ao5", "520 AO-IOChannel": "Dev1/ao4", "528 AO-TriggerSequenceLength": "1000", "405 Shutter-MaxVolts": "5.0000", "470-DA Device": "470 Shutter", "PRIME-FTCapable": "No", "Core-ChannelGroup": "Channels", "555 AO-SupportsTriggering": "0", "PRIME-Y-dimension": "1200", "Frame": 0, "555 Shutter-Sequenceable": "1", "PRIME-Timing-ExposureTimeNs": "499998000.0000", "Core-ImageProcessor": "", "528-State": "0", "520 AO-Block voltage": "false", "XLIGHT Excitation Wheel-Label": "CFP/YFP", "640 Shutter-Demo": "No", "COM3-StopBits": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "520 Shutter-IOChannel": "Dev1/ao11", "Width": 1200, "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-SupportsTriggering": "0", "640 AO-TriggerSampleRate": "100000", "520-Description": "DA device that is used as a shutter", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "405 Shutter-Sequenceable": "1", "405 Shutter-SupportsTriggering": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-TriggerSampleRate": "100000", "640 Shutter-MaxVolts": "5.0000", "555 AO-MaxVolts": "5.0000", "520 AO-TriggerSequenceLength": "1000", "COM3-BaudRate": "9600", "COM9-BaudRate": "9600", "Vincent-D3-Channel #": "Ch. #1", "PRIME-PMode": "Normal", "OpticalPath-Label": "Left(100)", "BitDepth": 16, "555 AO-TriggerSequenceLength": "1000", "445 Shutter-Description": "NI DAC", "405 AO-Sequenceable": "1", "PRIME-AcquisitionMethod": "Callbacks", "555 AO-Name": "AnalogIO", "PRIME-ShutterMode": "Pre-Exposure", "COM3-Handshaking": "Off", "445 AO-SupportsTriggering": "0", "ROI": "0-0-1200-1200", "COM9-Verbose": "1", "PRIME-Color": "OFF", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "PRIME-ShutterOpenDelay": "0", "COM1-Parity": "None", "Slice": 3, "SlicePosition": -20, "COM9-DelayBetweenCharsMs": "0.0000", "555-Name": "DA Shutter", "PIEZO-LudlSingleAxisName": "B", "XLIGHT Emission Wheel-Label": "565/24(G)", "640 Shutter-Name": "AnalogIO", "640 AO-Sequenceable": "1", "520 Shutter-Demo": "No", "528 Shutter-Demo": "No", "470-Name": "DA Shutter", "520-DA Device": "520 Shutter", "XYStage-Description": "Ludl XY stage driver adapter", "445 Shutter-MinVolts": "0.0000", "528 AO-TriggerSampleRate": "100000", "405 AO-MinVolts": "0.0000", "405 Shutter-TriggerSequenceLength": "1000", "Height": 1200, "555 AO-Block voltage": "false", "640 Shutter-Volts": "0.0000", "PixelType": "GRAY16", "445 Shutter-Block voltage": "false", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "520 Shutter-TriggerSequenceLength": "1000", "COM9-Name": "COM9", "Core-SLM": "", "555 AO-Volts": "0.0000", "555 Shutter-Block voltage": "false", "XYStage-Speed": "1800.0000", "520 AO-MaxVolts": "5.0000", "528 Shutter-SupportsTriggering": "0", "COM1-Description": "Serial port driver (boost:asio)", "PRIME-MetadataEnabled": "No", "520 Shutter-MinVolts": "0.0000", "470 Shutter-Sequenceable": "1", "PRIME-PreampDelay": "0", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-MinVolts": "0.0000", "ChannelIndex": 0, "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Core-AutoShutter": "1", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "Exposure-ms": 320, "PRIME-PP 2 ENABLED": "Yes", "640 Shutter-TriggerSampleRate": "100000", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "470 Shutter-IOChannel": "Dev1/ao10", "405 AO-Demo": "No", "PixelSizeUm": 1.1, "470 AO-Demo": "No", "470 Shutter-MaxVolts": "5.0000", "Vincent-D3-Name": "Vincent-D3", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FileName": "img_000000000_Il-G_003.tif", "LudlController-Reset": "Operate", "PRIME-Exposure": "320.00", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "445-Name": "DA Shutter", "555 Shutter-Name": "AnalogIO", "520 Shutter-TriggerSampleRate": "100000", "445 Shutter-IOChannel": "Dev1/ao9", "PRIME-TransposeCorrection": "0", "XYStage-StepSize": "0.1000", "445-Description": "DA device that is used as a shutter", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 AO-IOChannel": "Dev1/ao7", "520 Shutter-Name": "AnalogIO", "COM3-DataBits": "8", "640 AO-Volts": "0.0000", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "555 Shutter-IOChannel": "Dev1/ao13", "WaitInterval": null, "520 Shutter-Sequenceable": "1", "YPositionUm": 31506.2, "445-State": "0", "445-DA Device": "445 Shutter", "PRIME-CircularBufferAutoSize": "ON", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "555 AO-TriggerSampleRate": "100000", "445 Shutter-Volts": "0.0000", "XLIGHT Hub-Port": "COM3", "Core-AutoFocus": "", "COM4-Handshaking": "Off", "528 Shutter-MinVolts": "0.0000", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "445 Shutter-Name": "AnalogIO", "528 Shutter-Sequenceable": "1", "XYStage-TransposeMirrorX": "0", "XYStage-TransposeMirrorY": "0", "PRIME-ReadoutRate": "100MHz 16bit", "XYStage-Name": "Stage", "640 AO-SupportsTriggering": "0", "640-Name": "DA Shutter", "640 Shutter-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "640 AO-Block voltage": "false", "445 Shutter-Sequenceable": "1", "470 AO-MaxVolts": "5.0000", "COM4-BaudRate": "9600", "470-Description": "DA device that is used as a shutter", "520 Shutter-Description": "NI DAC", "PRIME-CCDTemperature": "-14.8500", "Time": "2019-12-18 21:36:43 -0500", "640 AO-TriggerSequenceLength": "1000", "470 AO-Name": "AnalogIO", "XLIGHT Disk slider-Label": "Disk Pos 40um", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "Binning": "1x1", "TE2000-ModelType": "1", "COM3-AnswerTimeout": "500.0000", "555 Shutter-SupportsTriggering": "0", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "TE2000-Port": "COM4", "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Description": "NI DAC", "COM4-AnswerTimeout": "500.0000", "COM1-DelayBetweenCharsMs": "0.0000", "FilterBlock-Label": "Confocal", "445 AO-Name": "AnalogIO", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-Timing-ReadoutTimeNs": "24672000.0000", "528 AO-Demo": "No", "520 AO-Sequenceable": "1", "555-Description": "DA device that is used as a shutter", "405-DA Device": "405 Shutter", "405 AO-TriggerSequenceLength": "1000", "640 AO-MinVolts": "0.0000", "640 Shutter-Block voltage": "false", "FrameIndex": 0, "PRIME-Port": "CMOS", "555 AO-MinVolts": "0.0000", "COM3-Description": "Serial port driver (boost:asio)", "528 AO-Description": "NI DAC", "555 AO-IOChannel": "Dev1/ao6", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "555 AO-TriggerInputLine": "/Dev1/PFI0", "PRIME-ActualInterval-ms": "390.5200", "LudlController-Port": "COM9", "445 Shutter-Demo": "No", "PRIME-Trigger-Expose Out-Mux": "1", "SliceIndex": 3, "PRIME-CCDTemperatureSetPoint": "-15.0000", "405 Shutter-MinVolts": "0.0000", "PRIME-ExposeOutMode": "First Row", "AxisPositions": null, "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Volts": "0.0000", "528-Name": "DA Shutter", "555-State": "0", "XLIGHT Dichroic-Label": "CFP/YFP", "445 AO-Sequenceable": "1", "405-Name": "DA Shutter", "405 AO-IOChannel": "Dev1/ao0", "Core-Camera": "PRIME", "Focus-Name": "Focus", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "405 Shutter-IOChannel": "Dev1/ao8", "640 AO-Demo": "No", "640 Shutter-SupportsTriggering": "0", "445 AO-Demo": "No", "555-DA Device": "555 Shutter", "470 Shutter-Description": "NI DAC", "528 AO-Volts": "0.0000", "COM1-DataBits": "8", "Vincent-D3-Address": "x", "520 AO-SupportsTriggering": "0", "405-Description": "DA device that is used as a shutter", "640 Shutter-MinVolts": "0.0000", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "405 AO-Name": "AnalogIO", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "640 AO-TriggerInputLine": "/Dev1/PFI0", "405 AO-TriggerInputLine": "/Dev1/PFI0", "640-Description": "DA device that is used as a shutter", "445 Shutter-TriggerSampleRate": "100000", "470 Shutter-TriggerSequenceLength": "1000", "COM1-StopBits": "1", "520 Shutter-SupportsTriggering": "0", "405-State": "0", "Core-Initialize": "1", "XLIGHT Emission Wheel-State": "3", "520 AO-Description": "NI DAC", "528 AO-TriggerInputLine": "/Dev1/PFI0", "445 Shutter-MaxVolts": "5.0000", "COM9-Handshaking": "Off", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "XLIGHT Spin motor-Label": "On (spinning)", "PRIME-PP 5 ENABLED": "No", "UUID": "d830aa8a-cb23-4b26-97f9-7dc00f70e07d", "PRIME-TriggerMode": "Internal Trigger", "COM1-Handshaking": "Off", "PRIME-Timing-PostTriggerDelayNs": "49344000.0000", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "Focus-Position": "17741.5000", "528 Shutter-Block voltage": "false", "Channel": "Il-G", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "555 Shutter-Demo": "No", "405 Shutter-Demo": "No", "528 AO-Name": "AnalogIO", "520-State": "1", "Core-Galvo": "", "405 Shutter-TriggerSampleRate": "100000", "Focus-FocusStepSize_(nm)": "100", "PRIME-PixelType": "16bit", "COM4-DataBits": "8", "470 AO-IOChannel": "Dev1/ao2", "520 AO-MinVolts": "0.0000", "COM9-Parity": "None", "PRIME-CircularBufferEnabled": "ON", "PRIME-PP 6 Reset": "No", "640 AO-Description": "NI DAC", "Source": "PRIME", "445 AO-MinVolts": "0.0000", "PIEZO-Name": "Stage", "445 AO-Description": "NI DAC", "PRIME-CircularBufferFrameCount": "46", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "520 AO-Volts": "4.5000", "528 Shutter-TriggerSampleRate": "100000", "XPositionUm": 19731.9, "OpticalPath-State": "4", "PRIME-PP 3 THRESHOLD": "80", "PRIME-PVCAM Version": "3.7.1", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Gain": "1-HDR", "640 Shutter-TriggerSequenceLength": "1000", "528 AO-SupportsTriggering": "0", "445 AO-TriggerInputLine": "/Dev1/PFI0", "OpticalPath-Name": "OpticalPath", "PRIME-PP 4 ENABLED": "Yes", "PRIME-PVCAM Adapter Version": "1.3.29", "COM3-Name": "COM3", "405 AO-MaxVolts": "5.0000", "520 Shutter-Block voltage": "false", "PRIME-Timing-ClearingTimeNs": "49344000.0000", "Core-Focus": "PIEZO", "528 AO-Block voltage": "false", "TE2000-Description": "Nikon TE2000 hub", "528 AO-MinVolts": "0.0000", "COM4-Parity": "None", "Summary": { "Slices": 11, "ChContrastMin": [ 0, 0, 0, 0, 0, 0 ], "PixelAspect": 1, "SlicesFirst": true, "Interval_ms": 0, "Time": "2019-12-18 21:36:42 -0500", "Prefix": "seq_4", "InitialPositionList": [ { "Label": "Pos0", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.21], "Focus": [17741.5], "XYStage": [ 44542.200000000004, 31636.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos1", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.22], "Focus": [17741.4], "XYStage": [ 43801.100000000006, 31637.9 ] }, "GridColumnIndex": 0 }, { "Label": "Pos2", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.5], "XYStage": [ 38561.3, 32040.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos3", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.185], "Focus": [17741.3], "XYStage": [ 37698.4, 32041.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos4", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.16], "Focus": [17741.5], "XYStage": [ 32375.4, 31758.9 ] }, "GridColumnIndex": 0 }, { "Label": "Pos5", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.17000000000002], "Focus": [17741.3], "XYStage": [ 31770.7, 31758.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos6", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.4], "XYStage": [ 26536.800000000003, 31068.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos7", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.205], "Focus": [17741.4], "XYStage": [ 25420.2, 31068.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos8", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.85], "Focus": [17741.3], "XYStage": [ 21059, 31508 ] }, "GridColumnIndex": 0 }, { "Label": "Pos9", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.82], "Focus": [17741.3], "XYStage": [ 19731.9, 31506.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos10", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.79], "Focus": [17741.4], "XYStage": [ 14528.5, 32007.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos11", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.83], "Focus": [17741.2], "XYStage": [ 13830.300000000001, 32009.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos12", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.79], "Focus": [17741.3], "XYStage": [ 8775.300000000001, 32017.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos13", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.815], "Focus": [17741.3], "XYStage": [ 8109.200000000001, 32008.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos14", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.805], "Focus": [17741.3], "XYStage": [ 3483.3, 31908.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos15", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.82], "Focus": [17741.2], "XYStage": [ 2686.8, 31903.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos16", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [211.48000000000002], "Focus": [17741.1], "XYStage": [ -1978.9, 31973.600000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos17", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [211.51500000000001], "Focus": [17741.1], "XYStage": [ -2957.8, 31982.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos18", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.195], "Focus": [17741.5], "XYStage": [ 47747.4, -576.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos19", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.23000000000002], "Focus": [17741.6], "XYStage": [ 47053.9, -576.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos20", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.205], "Focus": [17741.4], "XYStage": [ 41871.200000000004, -479.8 ] }, "GridColumnIndex": 0 }, { "Label": "Pos21", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.4], "XYStage": [ 40882.600000000006, -392 ] }, "GridColumnIndex": 0 }, { "Label": "Pos22", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.24], "Focus": [17741.4], "XYStage": [ 35905.6, -135.3 ] }, "GridColumnIndex": 0 }, { "Label": "Pos23", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.195], "Focus": [17741.5], "XYStage": [ 34990.9, -132.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos24", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.46], "Focus": [17741.4], "XYStage": [ 29678.100000000002, -958.1 ] }, "GridColumnIndex": 0 }, { "Label": "Pos25", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.44], "Focus": [17741.5], "XYStage": [ 28983.600000000002, -939.4000000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos26", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.475], "Focus": [17741.5], "XYStage": [ 23019.7, -122 ] }, "GridColumnIndex": 0 }, { "Label": "Pos27", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.44], "Focus": [17741.7], "XYStage": [ 22428, -116.80000000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos28", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.46], "Focus": [17741.7], "XYStage": [ 16364.900000000001, -181.10000000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos29", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.48000000000002], "Focus": [17741.5], "XYStage": [ 15712.1, -180 ] }, "GridColumnIndex": 0 }, { "Label": "Pos30", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.45000000000002], "Focus": [17741.7], "XYStage": [ 10346.1, 77.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos31", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.49], "Focus": [17741.4], "XYStage": [ 9755.5, 85.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos32", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.475], "Focus": [17741.5], "XYStage": [ 4775.5, 293.90000000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos33", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.445], "Focus": [17741.4], "XYStage": [ 3954.9, 293.90000000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos34", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.455], "Focus": [17741.4], "XYStage": [ -1324.9, 545.6 ] }, "GridColumnIndex": 0 }, { "Label": "Pos35", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.445], "Focus": [17741.3], "XYStage": [ -2097, 576.6 ] }, "GridColumnIndex": 0 } ], "z-step_um": 10, "GridRow": 0, "BitDepth": 16, "Depth": 2, "Channels": 6, "Source": "Micro-Manager", "ChColors": [ -16711681, -256, -65281, -1, -1, -16776961 ], "MetadataVersion": 10, "KeepShutterOpenChannels": true, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536, 65536 ], "CustomIntervals_ms": [], "Height": 1200, "MicroManagerVersion": "1.4.23 20180219", "GridColumn": 0, "UUID": "14a13bf7-c944-4672-b873-fcfbbb7ea13d", "PixelType": "GRAY16", "Width": 1200, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\Yan\\20191218-YC113-S7S8-miseq", "Frames": 1, "Comment": "", "UserName": "Nikon", "PixelSize_um": 1.1, "ROI": [ 0, 0, 1200, 1200 ], "Date": "2019-12-18", "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC", "P-DAPI-high" ], "KeepShutterOpenSlices": true, "IJType": 1, "ComputerName": "ZadorConfocal2", "Positions": 36, "PositionIndex": 9, "CameraTimeout": 20000, "TimeFirst": false }, "640 AO-Name": "AnalogIO", "Vincent-D3-Command": "Close" }, "FrameKey-0-0-4": { "COM4-StopBits": "1", "528 AO-MaxVolts": "5.0000", "XLIGHT Excitation Wheel-State": "1", "470 Shutter-Name": "AnalogIO", "Core-Shutter": "520", "555 AO-Demo": "No", "XLIGHT Dichroic-State": "2", "XYStage-StepSize-Y": "0.1000", "ZPositionUm": 238.82, "XYStage-StepSize-X": "0.1000", "PRIME-FirmwareVersion": "20.28", "PRIME-ClearCycles": "2", "520-Name": "DA Shutter", "405 Shutter-Name": "AnalogIO", "PIEZO-StepSize": "0.0050", "PRIME-ClearMode": "Pre-Exposure", "470 AO-MinVolts": "0.0000", "405 AO-SupportsTriggering": "1", "CameraChannelIndex": 0, "XYStage-StartSpeed": "500.0000", "COM9-StopBits": "2", "405 AO-Description": "NI DAC", "COM4-DelayBetweenCharsMs": "0.0000", "445 AO-Block voltage": "false", "520 AO-TriggerInputLine": "/Dev1/PFI0", "555 AO-Description": "NI DAC", "520 AO-TriggerSampleRate": "100000", "COM4-Verbose": "1", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM4-Name": "COM4", "405 Shutter-Volts": "0.0000", "Vincent-D3-Port": "COM1", "PRIME-PP 4 MIN ADU AFFECTED": "200", "470 Shutter-MinVolts": "0.0000", "PRIME-PP 3 ENABLED": "Yes", "470 AO-Description": "NI DAC", "PRIME-Description": "PVCAM API device adapter", "520 Shutter-MaxVolts": "5.0000", "520 Shutter-Volts": "5.0000", "528 AO-Sequenceable": "1", "PRIME-Binning": "1x1", "555 Shutter-MaxVolts": "5.0000", "528 Shutter-TriggerSequenceLength": "1000", "405 Shutter-Block voltage": "false", "XLIGHT Spin motor-State": "1", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "Vincent-D3-Time to open (ms)": "35.0000", "Vincent-D3-Time to close (ms)": "35.0000", "445 AO-TriggerSampleRate": "100000", "640 Shutter-IOChannel": "Dev1/ao14", "555 AO-Sequenceable": "1", "520 AO-Name": "AnalogIO", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "470 AO-Block voltage": "false", "COM1-Verbose": "1", "COM9-AnswerTimeout": "10000.0000", "528 Shutter-Name": "AnalogIO", "PRIME-Trigger Timeout (secs)": "10", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "NextFrame": 0, "470 AO-TriggerSequenceLength": "1000", "PRIME-TransposeXY": "0", "445 AO-TriggerSequenceLength": "1000", "COM1-Name": "COM1", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "COM9-DataBits": "8", "COM1-AnswerTimeout": "500.0000", "Camera": "", "TE2000-Version": "V2.50-V2.50-V2.10", "528 Shutter-Volts": "0.0000", "XLIGHT Hub-Name": "XLIGHT Hub", "PIEZO-Description": "Ludl stage driver adapter", "PositionName": "Pos9", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSampleRate": "100000", "PRIME-FanSpeedSetpoint": "High", "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "405 AO-TriggerSampleRate": "100000", "470 Shutter-Block voltage": "false", "470-State": "0", "COM3-Parity": "None", "405 AO-Volts": "0.0000", "PRIME-TransposeMirrorY": "0", "PRIME-ChipName": "GS144BSI", "470 AO-Volts": "0.0000", "PRIME-TransposeMirrorX": "0", "445 Shutter-SupportsTriggering": "0", "470 Shutter-SupportsTriggering": "0", "XLIGHT Hub-Description": "XLIGHT hub", "PRIME-PreampOffLimit": "10000", "470 AO-Sequenceable": "1", "528 Shutter-MaxVolts": "5.0000", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "Core-XYStage": "XYStage", "470 AO-TriggerSampleRate": "100000", "PRIME-Timing-PreTriggerDelayNs": "24672000.0000", "528-DA Device": "528 Shutter", "Focus-Description": "Nikon TE2000 focus adapter", "FilterBlock-State": "5", "520 AO-Demo": "No", "COM3-DelayBetweenCharsMs": "0.0000", "470 Shutter-Volts": "0.0000", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "PRIME-PP 2 THRESHOLD": "125", "PRIME-PP 4 THRESHOLD": "75", "XYStage-Acceleration": "4.0000", "470 Shutter-Demo": "No", "PRIME-Name": "PMPCIECam00", "640-DA Device": "640 Shutter", "405 AO-Block voltage": "false", "ElapsedTime-ms": 376625, "PRIME-Offset": "100", "445 AO-IOChannel": "Dev1/ao1", "640 AO-MaxVolts": "5.0000", "PositionIndex": 9, "PRIME-PP 1 ENABLED": "Yes", "COM1-BaudRate": "9600", "640-State": "0", "COM3-Verbose": "1", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "LudlController-TransmissionDelay": "0", "PRIME-FullWellCapacity": "62000", "PRIME-SMARTStreamingEnabled": "No", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "640 Shutter-Description": "NI DAC", "528-Description": "DA device that is used as a shutter", "COM4-Description": "Serial port driver (boost:asio)", "405 Shutter-Description": "NI DAC", "TE2000-Name": "TE2000", "528 Shutter-Description": "NI DAC", "COM9-Description": "Serial port driver (boost:asio)", "LudlController-Name": "LudlController", "445 Shutter-TriggerSequenceLength": "1000", "PRIME-SerialNumber": "A18B203008", "445 AO-Volts": "0.0000", "PRIME-X-dimension": "1200", "PRIME-ShutterCloseDelay": "0", "XLIGHT Disk slider-State": "2", "Position": "Default", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 AO-IOChannel": "Dev1/ao5", "520 AO-IOChannel": "Dev1/ao4", "528 AO-TriggerSequenceLength": "1000", "405 Shutter-MaxVolts": "5.0000", "470-DA Device": "470 Shutter", "PRIME-FTCapable": "No", "Core-ChannelGroup": "Channels", "555 AO-SupportsTriggering": "0", "PRIME-Y-dimension": "1200", "Frame": 0, "555 Shutter-Sequenceable": "1", "PRIME-Timing-ExposureTimeNs": "499998000.0000", "Core-ImageProcessor": "", "528-State": "0", "520 AO-Block voltage": "false", "XLIGHT Excitation Wheel-Label": "CFP/YFP", "640 Shutter-Demo": "No", "COM3-StopBits": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "520 Shutter-IOChannel": "Dev1/ao11", "Width": 1200, "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-SupportsTriggering": "0", "640 AO-TriggerSampleRate": "100000", "520-Description": "DA device that is used as a shutter", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "405 Shutter-Sequenceable": "1", "405 Shutter-SupportsTriggering": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-TriggerSampleRate": "100000", "640 Shutter-MaxVolts": "5.0000", "555 AO-MaxVolts": "5.0000", "520 AO-TriggerSequenceLength": "1000", "COM3-BaudRate": "9600", "COM9-BaudRate": "9600", "Vincent-D3-Channel #": "Ch. #1", "PRIME-PMode": "Normal", "OpticalPath-Label": "Left(100)", "BitDepth": 16, "555 AO-TriggerSequenceLength": "1000", "445 Shutter-Description": "NI DAC", "405 AO-Sequenceable": "1", "PRIME-AcquisitionMethod": "Callbacks", "555 AO-Name": "AnalogIO", "PRIME-ShutterMode": "Pre-Exposure", "COM3-Handshaking": "Off", "445 AO-SupportsTriggering": "0", "ROI": "0-0-1200-1200", "COM9-Verbose": "1", "PRIME-Color": "OFF", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "PRIME-ShutterOpenDelay": "0", "COM1-Parity": "None", "Slice": 4, "SlicePosition": -10, "COM9-DelayBetweenCharsMs": "0.0000", "555-Name": "DA Shutter", "PIEZO-LudlSingleAxisName": "B", "XLIGHT Emission Wheel-Label": "565/24(G)", "640 Shutter-Name": "AnalogIO", "640 AO-Sequenceable": "1", "520 Shutter-Demo": "No", "528 Shutter-Demo": "No", "470-Name": "DA Shutter", "520-DA Device": "520 Shutter", "XYStage-Description": "Ludl XY stage driver adapter", "445 Shutter-MinVolts": "0.0000", "528 AO-TriggerSampleRate": "100000", "405 AO-MinVolts": "0.0000", "405 Shutter-TriggerSequenceLength": "1000", "Height": 1200, "555 AO-Block voltage": "false", "640 Shutter-Volts": "0.0000", "PixelType": "GRAY16", "445 Shutter-Block voltage": "false", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "520 Shutter-TriggerSequenceLength": "1000", "COM9-Name": "COM9", "Core-SLM": "", "555 AO-Volts": "0.0000", "555 Shutter-Block voltage": "false", "XYStage-Speed": "1800.0000", "520 AO-MaxVolts": "5.0000", "528 Shutter-SupportsTriggering": "0", "COM1-Description": "Serial port driver (boost:asio)", "PRIME-MetadataEnabled": "No", "520 Shutter-MinVolts": "0.0000", "470 Shutter-Sequenceable": "1", "PRIME-PreampDelay": "0", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-MinVolts": "0.0000", "ChannelIndex": 0, "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Core-AutoShutter": "1", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "Exposure-ms": 320, "PRIME-PP 2 ENABLED": "Yes", "640 Shutter-TriggerSampleRate": "100000", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "470 Shutter-IOChannel": "Dev1/ao10", "405 AO-Demo": "No", "PixelSizeUm": 1.1, "470 AO-Demo": "No", "470 Shutter-MaxVolts": "5.0000", "Vincent-D3-Name": "Vincent-D3", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FileName": "img_000000000_Il-G_004.tif", "LudlController-Reset": "Operate", "PRIME-Exposure": "320.00", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "445-Name": "DA Shutter", "555 Shutter-Name": "AnalogIO", "520 Shutter-TriggerSampleRate": "100000", "445 Shutter-IOChannel": "Dev1/ao9", "PRIME-TransposeCorrection": "0", "XYStage-StepSize": "0.1000", "445-Description": "DA device that is used as a shutter", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 AO-IOChannel": "Dev1/ao7", "520 Shutter-Name": "AnalogIO", "COM3-DataBits": "8", "640 AO-Volts": "0.0000", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "555 Shutter-IOChannel": "Dev1/ao13", "WaitInterval": null, "520 Shutter-Sequenceable": "1", "YPositionUm": 31506.2, "445-State": "0", "445-DA Device": "445 Shutter", "PRIME-CircularBufferAutoSize": "ON", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "555 AO-TriggerSampleRate": "100000", "445 Shutter-Volts": "0.0000", "XLIGHT Hub-Port": "COM3", "Core-AutoFocus": "", "COM4-Handshaking": "Off", "528 Shutter-MinVolts": "0.0000", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "445 Shutter-Name": "AnalogIO", "528 Shutter-Sequenceable": "1", "XYStage-TransposeMirrorX": "0", "XYStage-TransposeMirrorY": "0", "PRIME-ReadoutRate": "100MHz 16bit", "XYStage-Name": "Stage", "640 AO-SupportsTriggering": "0", "640-Name": "DA Shutter", "640 Shutter-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "640 AO-Block voltage": "false", "445 Shutter-Sequenceable": "1", "470 AO-MaxVolts": "5.0000", "COM4-BaudRate": "9600", "470-Description": "DA device that is used as a shutter", "520 Shutter-Description": "NI DAC", "PRIME-CCDTemperature": "-14.8500", "Time": "2019-12-18 21:36:44 -0500", "640 AO-TriggerSequenceLength": "1000", "470 AO-Name": "AnalogIO", "XLIGHT Disk slider-Label": "Disk Pos 40um", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "Binning": "1x1", "TE2000-ModelType": "1", "COM3-AnswerTimeout": "500.0000", "555 Shutter-SupportsTriggering": "0", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "TE2000-Port": "COM4", "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Description": "NI DAC", "COM4-AnswerTimeout": "500.0000", "COM1-DelayBetweenCharsMs": "0.0000", "FilterBlock-Label": "Confocal", "445 AO-Name": "AnalogIO", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-Timing-ReadoutTimeNs": "24672000.0000", "528 AO-Demo": "No", "520 AO-Sequenceable": "1", "555-Description": "DA device that is used as a shutter", "405-DA Device": "405 Shutter", "405 AO-TriggerSequenceLength": "1000", "640 AO-MinVolts": "0.0000", "640 Shutter-Block voltage": "false", "FrameIndex": 0, "PRIME-Port": "CMOS", "555 AO-MinVolts": "0.0000", "COM3-Description": "Serial port driver (boost:asio)", "528 AO-Description": "NI DAC", "555 AO-IOChannel": "Dev1/ao6", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "555 AO-TriggerInputLine": "/Dev1/PFI0", "PRIME-ActualInterval-ms": "390.5200", "LudlController-Port": "COM9", "445 Shutter-Demo": "No", "PRIME-Trigger-Expose Out-Mux": "1", "SliceIndex": 4, "PRIME-CCDTemperatureSetPoint": "-15.0000", "405 Shutter-MinVolts": "0.0000", "PRIME-ExposeOutMode": "First Row", "AxisPositions": null, "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Volts": "0.0000", "528-Name": "DA Shutter", "555-State": "0", "XLIGHT Dichroic-Label": "CFP/YFP", "445 AO-Sequenceable": "1", "405-Name": "DA Shutter", "405 AO-IOChannel": "Dev1/ao0", "Core-Camera": "PRIME", "Focus-Name": "Focus", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "405 Shutter-IOChannel": "Dev1/ao8", "640 AO-Demo": "No", "640 Shutter-SupportsTriggering": "0", "445 AO-Demo": "No", "555-DA Device": "555 Shutter", "470 Shutter-Description": "NI DAC", "528 AO-Volts": "0.0000", "COM1-DataBits": "8", "Vincent-D3-Address": "x", "520 AO-SupportsTriggering": "0", "405-Description": "DA device that is used as a shutter", "640 Shutter-MinVolts": "0.0000", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "405 AO-Name": "AnalogIO", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "640 AO-TriggerInputLine": "/Dev1/PFI0", "405 AO-TriggerInputLine": "/Dev1/PFI0", "640-Description": "DA device that is used as a shutter", "445 Shutter-TriggerSampleRate": "100000", "470 Shutter-TriggerSequenceLength": "1000", "COM1-StopBits": "1", "520 Shutter-SupportsTriggering": "0", "405-State": "0", "Core-Initialize": "1", "XLIGHT Emission Wheel-State": "3", "520 AO-Description": "NI DAC", "528 AO-TriggerInputLine": "/Dev1/PFI0", "445 Shutter-MaxVolts": "5.0000", "COM9-Handshaking": "Off", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "XLIGHT Spin motor-Label": "On (spinning)", "PRIME-PP 5 ENABLED": "No", "UUID": "cedab12f-e264-43a4-92eb-fd841220084c", "PRIME-TriggerMode": "Internal Trigger", "COM1-Handshaking": "Off", "PRIME-Timing-PostTriggerDelayNs": "49344000.0000", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "Focus-Position": "17741.5000", "528 Shutter-Block voltage": "false", "Channel": "Il-G", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "555 Shutter-Demo": "No", "405 Shutter-Demo": "No", "528 AO-Name": "AnalogIO", "520-State": "1", "Core-Galvo": "", "405 Shutter-TriggerSampleRate": "100000", "Focus-FocusStepSize_(nm)": "100", "PRIME-PixelType": "16bit", "COM4-DataBits": "8", "470 AO-IOChannel": "Dev1/ao2", "520 AO-MinVolts": "0.0000", "COM9-Parity": "None", "PRIME-CircularBufferEnabled": "ON", "PRIME-PP 6 Reset": "No", "640 AO-Description": "NI DAC", "Source": "PRIME", "445 AO-MinVolts": "0.0000", "PIEZO-Name": "Stage", "445 AO-Description": "NI DAC", "PRIME-CircularBufferFrameCount": "46", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "520 AO-Volts": "4.5000", "528 Shutter-TriggerSampleRate": "100000", "XPositionUm": 19731.9, "OpticalPath-State": "4", "PRIME-PP 3 THRESHOLD": "80", "PRIME-PVCAM Version": "3.7.1", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Gain": "1-HDR", "640 Shutter-TriggerSequenceLength": "1000", "528 AO-SupportsTriggering": "0", "445 AO-TriggerInputLine": "/Dev1/PFI0", "OpticalPath-Name": "OpticalPath", "PRIME-PP 4 ENABLED": "Yes", "PRIME-PVCAM Adapter Version": "1.3.29", "COM3-Name": "COM3", "405 AO-MaxVolts": "5.0000", "520 Shutter-Block voltage": "false", "PRIME-Timing-ClearingTimeNs": "49344000.0000", "Core-Focus": "PIEZO", "528 AO-Block voltage": "false", "TE2000-Description": "Nikon TE2000 hub", "528 AO-MinVolts": "0.0000", "COM4-Parity": "None", "Summary": { "Slices": 11, "ChContrastMin": [ 0, 0, 0, 0, 0, 0 ], "PixelAspect": 1, "SlicesFirst": true, "Interval_ms": 0, "Time": "2019-12-18 21:36:42 -0500", "Prefix": "seq_4", "InitialPositionList": [ { "Label": "Pos0", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.21], "Focus": [17741.5], "XYStage": [ 44542.200000000004, 31636.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos1", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.22], "Focus": [17741.4], "XYStage": [ 43801.100000000006, 31637.9 ] }, "GridColumnIndex": 0 }, { "Label": "Pos2", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.5], "XYStage": [ 38561.3, 32040.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos3", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.185], "Focus": [17741.3], "XYStage": [ 37698.4, 32041.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos4", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.16], "Focus": [17741.5], "XYStage": [ 32375.4, 31758.9 ] }, "GridColumnIndex": 0 }, { "Label": "Pos5", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.17000000000002], "Focus": [17741.3], "XYStage": [ 31770.7, 31758.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos6", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.4], "XYStage": [ 26536.800000000003, 31068.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos7", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.205], "Focus": [17741.4], "XYStage": [ 25420.2, 31068.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos8", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.85], "Focus": [17741.3], "XYStage": [ 21059, 31508 ] }, "GridColumnIndex": 0 }, { "Label": "Pos9", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.82], "Focus": [17741.3], "XYStage": [ 19731.9, 31506.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos10", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.79], "Focus": [17741.4], "XYStage": [ 14528.5, 32007.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos11", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.83], "Focus": [17741.2], "XYStage": [ 13830.300000000001, 32009.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos12", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.79], "Focus": [17741.3], "XYStage": [ 8775.300000000001, 32017.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos13", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.815], "Focus": [17741.3], "XYStage": [ 8109.200000000001, 32008.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos14", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.805], "Focus": [17741.3], "XYStage": [ 3483.3, 31908.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos15", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.82], "Focus": [17741.2], "XYStage": [ 2686.8, 31903.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos16", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [211.48000000000002], "Focus": [17741.1], "XYStage": [ -1978.9, 31973.600000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos17", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [211.51500000000001], "Focus": [17741.1], "XYStage": [ -2957.8, 31982.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos18", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.195], "Focus": [17741.5], "XYStage": [ 47747.4, -576.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos19", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.23000000000002], "Focus": [17741.6], "XYStage": [ 47053.9, -576.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos20", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.205], "Focus": [17741.4], "XYStage": [ 41871.200000000004, -479.8 ] }, "GridColumnIndex": 0 }, { "Label": "Pos21", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.4], "XYStage": [ 40882.600000000006, -392 ] }, "GridColumnIndex": 0 }, { "Label": "Pos22", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.24], "Focus": [17741.4], "XYStage": [ 35905.6, -135.3 ] }, "GridColumnIndex": 0 }, { "Label": "Pos23", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.195], "Focus": [17741.5], "XYStage": [ 34990.9, -132.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos24", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.46], "Focus": [17741.4], "XYStage": [ 29678.100000000002, -958.1 ] }, "GridColumnIndex": 0 }, { "Label": "Pos25", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.44], "Focus": [17741.5], "XYStage": [ 28983.600000000002, -939.4000000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos26", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.475], "Focus": [17741.5], "XYStage": [ 23019.7, -122 ] }, "GridColumnIndex": 0 }, { "Label": "Pos27", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.44], "Focus": [17741.7], "XYStage": [ 22428, -116.80000000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos28", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.46], "Focus": [17741.7], "XYStage": [ 16364.900000000001, -181.10000000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos29", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.48000000000002], "Focus": [17741.5], "XYStage": [ 15712.1, -180 ] }, "GridColumnIndex": 0 }, { "Label": "Pos30", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.45000000000002], "Focus": [17741.7], "XYStage": [ 10346.1, 77.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos31", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.49], "Focus": [17741.4], "XYStage": [ 9755.5, 85.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos32", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.475], "Focus": [17741.5], "XYStage": [ 4775.5, 293.90000000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos33", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.445], "Focus": [17741.4], "XYStage": [ 3954.9, 293.90000000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos34", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.455], "Focus": [17741.4], "XYStage": [ -1324.9, 545.6 ] }, "GridColumnIndex": 0 }, { "Label": "Pos35", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.445], "Focus": [17741.3], "XYStage": [ -2097, 576.6 ] }, "GridColumnIndex": 0 } ], "z-step_um": 10, "GridRow": 0, "BitDepth": 16, "Depth": 2, "Channels": 6, "Source": "Micro-Manager", "ChColors": [ -16711681, -256, -65281, -1, -1, -16776961 ], "MetadataVersion": 10, "KeepShutterOpenChannels": true, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536, 65536 ], "CustomIntervals_ms": [], "Height": 1200, "MicroManagerVersion": "1.4.23 20180219", "GridColumn": 0, "UUID": "14a13bf7-c944-4672-b873-fcfbbb7ea13d", "PixelType": "GRAY16", "Width": 1200, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\Yan\\20191218-YC113-S7S8-miseq", "Frames": 1, "Comment": "", "UserName": "Nikon", "PixelSize_um": 1.1, "ROI": [ 0, 0, 1200, 1200 ], "Date": "2019-12-18", "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC", "P-DAPI-high" ], "KeepShutterOpenSlices": true, "IJType": 1, "ComputerName": "ZadorConfocal2", "Positions": 36, "PositionIndex": 9, "CameraTimeout": 20000, "TimeFirst": false }, "640 AO-Name": "AnalogIO", "Vincent-D3-Command": "Close" }, "FrameKey-0-0-5": { "COM4-StopBits": "1", "528 AO-MaxVolts": "5.0000", "XLIGHT Excitation Wheel-State": "1", "470 Shutter-Name": "AnalogIO", "Core-Shutter": "520", "555 AO-Demo": "No", "XLIGHT Dichroic-State": "2", "XYStage-StepSize-Y": "0.1000", "ZPositionUm": 248.82, "XYStage-StepSize-X": "0.1000", "PRIME-FirmwareVersion": "20.28", "PRIME-ClearCycles": "2", "520-Name": "DA Shutter", "405 Shutter-Name": "AnalogIO", "PIEZO-StepSize": "0.0050", "PRIME-ClearMode": "Pre-Exposure", "470 AO-MinVolts": "0.0000", "405 AO-SupportsTriggering": "1", "CameraChannelIndex": 0, "XYStage-StartSpeed": "500.0000", "COM9-StopBits": "2", "405 AO-Description": "NI DAC", "COM4-DelayBetweenCharsMs": "0.0000", "445 AO-Block voltage": "false", "520 AO-TriggerInputLine": "/Dev1/PFI0", "555 AO-Description": "NI DAC", "520 AO-TriggerSampleRate": "100000", "COM4-Verbose": "1", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM4-Name": "COM4", "405 Shutter-Volts": "0.0000", "Vincent-D3-Port": "COM1", "PRIME-PP 4 MIN ADU AFFECTED": "200", "470 Shutter-MinVolts": "0.0000", "PRIME-PP 3 ENABLED": "Yes", "470 AO-Description": "NI DAC", "PRIME-Description": "PVCAM API device adapter", "520 Shutter-MaxVolts": "5.0000", "520 Shutter-Volts": "5.0000", "528 AO-Sequenceable": "1", "PRIME-Binning": "1x1", "555 Shutter-MaxVolts": "5.0000", "528 Shutter-TriggerSequenceLength": "1000", "405 Shutter-Block voltage": "false", "XLIGHT Spin motor-State": "1", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "Vincent-D3-Time to open (ms)": "35.0000", "Vincent-D3-Time to close (ms)": "35.0000", "445 AO-TriggerSampleRate": "100000", "640 Shutter-IOChannel": "Dev1/ao14", "555 AO-Sequenceable": "1", "520 AO-Name": "AnalogIO", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "470 AO-Block voltage": "false", "COM1-Verbose": "1", "COM9-AnswerTimeout": "10000.0000", "528 Shutter-Name": "AnalogIO", "PRIME-Trigger Timeout (secs)": "10", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "NextFrame": 0, "470 AO-TriggerSequenceLength": "1000", "PRIME-TransposeXY": "0", "445 AO-TriggerSequenceLength": "1000", "COM1-Name": "COM1", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "COM9-DataBits": "8", "COM1-AnswerTimeout": "500.0000", "Camera": "", "TE2000-Version": "V2.50-V2.50-V2.10", "528 Shutter-Volts": "0.0000", "XLIGHT Hub-Name": "XLIGHT Hub", "PIEZO-Description": "Ludl stage driver adapter", "PositionName": "Pos9", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSampleRate": "100000", "PRIME-FanSpeedSetpoint": "High", "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "405 AO-TriggerSampleRate": "100000", "470 Shutter-Block voltage": "false", "470-State": "0", "COM3-Parity": "None", "405 AO-Volts": "0.0000", "PRIME-TransposeMirrorY": "0", "PRIME-ChipName": "GS144BSI", "470 AO-Volts": "0.0000", "PRIME-TransposeMirrorX": "0", "445 Shutter-SupportsTriggering": "0", "470 Shutter-SupportsTriggering": "0", "XLIGHT Hub-Description": "XLIGHT hub", "PRIME-PreampOffLimit": "10000", "470 AO-Sequenceable": "1", "528 Shutter-MaxVolts": "5.0000", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "Core-XYStage": "XYStage", "470 AO-TriggerSampleRate": "100000", "PRIME-Timing-PreTriggerDelayNs": "24672000.0000", "528-DA Device": "528 Shutter", "Focus-Description": "Nikon TE2000 focus adapter", "FilterBlock-State": "5", "520 AO-Demo": "No", "COM3-DelayBetweenCharsMs": "0.0000", "470 Shutter-Volts": "0.0000", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "PRIME-PP 2 THRESHOLD": "125", "PRIME-PP 4 THRESHOLD": "75", "XYStage-Acceleration": "4.0000", "470 Shutter-Demo": "No", "PRIME-Name": "PMPCIECam00", "640-DA Device": "640 Shutter", "405 AO-Block voltage": "false", "ElapsedTime-ms": 377231, "PRIME-Offset": "100", "445 AO-IOChannel": "Dev1/ao1", "640 AO-MaxVolts": "5.0000", "PositionIndex": 9, "PRIME-PP 1 ENABLED": "Yes", "COM1-BaudRate": "9600", "640-State": "0", "COM3-Verbose": "1", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "LudlController-TransmissionDelay": "0", "PRIME-FullWellCapacity": "62000", "PRIME-SMARTStreamingEnabled": "No", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "640 Shutter-Description": "NI DAC", "528-Description": "DA device that is used as a shutter", "COM4-Description": "Serial port driver (boost:asio)", "405 Shutter-Description": "NI DAC", "TE2000-Name": "TE2000", "528 Shutter-Description": "NI DAC", "COM9-Description": "Serial port driver (boost:asio)", "LudlController-Name": "LudlController", "445 Shutter-TriggerSequenceLength": "1000", "PRIME-SerialNumber": "A18B203008", "445 AO-Volts": "0.0000", "PRIME-X-dimension": "1200", "PRIME-ShutterCloseDelay": "0", "XLIGHT Disk slider-State": "2", "Position": "Default", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 AO-IOChannel": "Dev1/ao5", "520 AO-IOChannel": "Dev1/ao4", "528 AO-TriggerSequenceLength": "1000", "405 Shutter-MaxVolts": "5.0000", "470-DA Device": "470 Shutter", "PRIME-FTCapable": "No", "Core-ChannelGroup": "Channels", "555 AO-SupportsTriggering": "0", "PRIME-Y-dimension": "1200", "Frame": 0, "555 Shutter-Sequenceable": "1", "PRIME-Timing-ExposureTimeNs": "499998000.0000", "Core-ImageProcessor": "", "528-State": "0", "520 AO-Block voltage": "false", "XLIGHT Excitation Wheel-Label": "CFP/YFP", "640 Shutter-Demo": "No", "COM3-StopBits": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "520 Shutter-IOChannel": "Dev1/ao11", "Width": 1200, "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-SupportsTriggering": "0", "640 AO-TriggerSampleRate": "100000", "520-Description": "DA device that is used as a shutter", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "405 Shutter-Sequenceable": "1", "405 Shutter-SupportsTriggering": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-TriggerSampleRate": "100000", "640 Shutter-MaxVolts": "5.0000", "555 AO-MaxVolts": "5.0000", "520 AO-TriggerSequenceLength": "1000", "COM3-BaudRate": "9600", "COM9-BaudRate": "9600", "Vincent-D3-Channel #": "Ch. #1", "PRIME-PMode": "Normal", "OpticalPath-Label": "Left(100)", "BitDepth": 16, "555 AO-TriggerSequenceLength": "1000", "445 Shutter-Description": "NI DAC", "405 AO-Sequenceable": "1", "PRIME-AcquisitionMethod": "Callbacks", "555 AO-Name": "AnalogIO", "PRIME-ShutterMode": "Pre-Exposure", "COM3-Handshaking": "Off", "445 AO-SupportsTriggering": "0", "ROI": "0-0-1200-1200", "COM9-Verbose": "1", "PRIME-Color": "OFF", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "PRIME-ShutterOpenDelay": "0", "COM1-Parity": "None", "Slice": 5, "SlicePosition": 0, "COM9-DelayBetweenCharsMs": "0.0000", "555-Name": "DA Shutter", "PIEZO-LudlSingleAxisName": "B", "XLIGHT Emission Wheel-Label": "565/24(G)", "640 Shutter-Name": "AnalogIO", "640 AO-Sequenceable": "1", "520 Shutter-Demo": "No", "528 Shutter-Demo": "No", "470-Name": "DA Shutter", "520-DA Device": "520 Shutter", "XYStage-Description": "Ludl XY stage driver adapter", "445 Shutter-MinVolts": "0.0000", "528 AO-TriggerSampleRate": "100000", "405 AO-MinVolts": "0.0000", "405 Shutter-TriggerSequenceLength": "1000", "Height": 1200, "555 AO-Block voltage": "false", "640 Shutter-Volts": "0.0000", "PixelType": "GRAY16", "445 Shutter-Block voltage": "false", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "520 Shutter-TriggerSequenceLength": "1000", "COM9-Name": "COM9", "Core-SLM": "", "555 AO-Volts": "0.0000", "555 Shutter-Block voltage": "false", "XYStage-Speed": "1800.0000", "520 AO-MaxVolts": "5.0000", "528 Shutter-SupportsTriggering": "0", "COM1-Description": "Serial port driver (boost:asio)", "PRIME-MetadataEnabled": "No", "520 Shutter-MinVolts": "0.0000", "470 Shutter-Sequenceable": "1", "PRIME-PreampDelay": "0", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-MinVolts": "0.0000", "ChannelIndex": 0, "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Core-AutoShutter": "1", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "Exposure-ms": 320, "PRIME-PP 2 ENABLED": "Yes", "640 Shutter-TriggerSampleRate": "100000", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "470 Shutter-IOChannel": "Dev1/ao10", "405 AO-Demo": "No", "PixelSizeUm": 1.1, "470 AO-Demo": "No", "470 Shutter-MaxVolts": "5.0000", "Vincent-D3-Name": "Vincent-D3", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FileName": "img_000000000_Il-G_005.tif", "LudlController-Reset": "Operate", "PRIME-Exposure": "320.00", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "445-Name": "DA Shutter", "555 Shutter-Name": "AnalogIO", "520 Shutter-TriggerSampleRate": "100000", "445 Shutter-IOChannel": "Dev1/ao9", "PRIME-TransposeCorrection": "0", "XYStage-StepSize": "0.1000", "445-Description": "DA device that is used as a shutter", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 AO-IOChannel": "Dev1/ao7", "520 Shutter-Name": "AnalogIO", "COM3-DataBits": "8", "640 AO-Volts": "0.0000", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "555 Shutter-IOChannel": "Dev1/ao13", "WaitInterval": null, "520 Shutter-Sequenceable": "1", "YPositionUm": 31506.2, "445-State": "0", "445-DA Device": "445 Shutter", "PRIME-CircularBufferAutoSize": "ON", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "555 AO-TriggerSampleRate": "100000", "445 Shutter-Volts": "0.0000", "XLIGHT Hub-Port": "COM3", "Core-AutoFocus": "", "COM4-Handshaking": "Off", "528 Shutter-MinVolts": "0.0000", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "445 Shutter-Name": "AnalogIO", "528 Shutter-Sequenceable": "1", "XYStage-TransposeMirrorX": "0", "XYStage-TransposeMirrorY": "0", "PRIME-ReadoutRate": "100MHz 16bit", "XYStage-Name": "Stage", "640 AO-SupportsTriggering": "0", "640-Name": "DA Shutter", "640 Shutter-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "640 AO-Block voltage": "false", "445 Shutter-Sequenceable": "1", "470 AO-MaxVolts": "5.0000", "COM4-BaudRate": "9600", "470-Description": "DA device that is used as a shutter", "520 Shutter-Description": "NI DAC", "PRIME-CCDTemperature": "-14.8500", "Time": "2019-12-18 21:36:45 -0500", "640 AO-TriggerSequenceLength": "1000", "470 AO-Name": "AnalogIO", "XLIGHT Disk slider-Label": "Disk Pos 40um", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "Binning": "1x1", "TE2000-ModelType": "1", "COM3-AnswerTimeout": "500.0000", "555 Shutter-SupportsTriggering": "0", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "TE2000-Port": "COM4", "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Description": "NI DAC", "COM4-AnswerTimeout": "500.0000", "COM1-DelayBetweenCharsMs": "0.0000", "FilterBlock-Label": "Confocal", "445 AO-Name": "AnalogIO", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-Timing-ReadoutTimeNs": "24672000.0000", "528 AO-Demo": "No", "520 AO-Sequenceable": "1", "555-Description": "DA device that is used as a shutter", "405-DA Device": "405 Shutter", "405 AO-TriggerSequenceLength": "1000", "640 AO-MinVolts": "0.0000", "640 Shutter-Block voltage": "false", "FrameIndex": 0, "PRIME-Port": "CMOS", "555 AO-MinVolts": "0.0000", "COM3-Description": "Serial port driver (boost:asio)", "528 AO-Description": "NI DAC", "555 AO-IOChannel": "Dev1/ao6", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "555 AO-TriggerInputLine": "/Dev1/PFI0", "PRIME-ActualInterval-ms": "390.5200", "LudlController-Port": "COM9", "445 Shutter-Demo": "No", "PRIME-Trigger-Expose Out-Mux": "1", "SliceIndex": 5, "PRIME-CCDTemperatureSetPoint": "-15.0000", "405 Shutter-MinVolts": "0.0000", "PRIME-ExposeOutMode": "First Row", "AxisPositions": null, "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Volts": "0.0000", "528-Name": "DA Shutter", "555-State": "0", "XLIGHT Dichroic-Label": "CFP/YFP", "445 AO-Sequenceable": "1", "405-Name": "DA Shutter", "405 AO-IOChannel": "Dev1/ao0", "Core-Camera": "PRIME", "Focus-Name": "Focus", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "405 Shutter-IOChannel": "Dev1/ao8", "640 AO-Demo": "No", "640 Shutter-SupportsTriggering": "0", "445 AO-Demo": "No", "555-DA Device": "555 Shutter", "470 Shutter-Description": "NI DAC", "528 AO-Volts": "0.0000", "COM1-DataBits": "8", "Vincent-D3-Address": "x", "520 AO-SupportsTriggering": "0", "405-Description": "DA device that is used as a shutter", "640 Shutter-MinVolts": "0.0000", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "405 AO-Name": "AnalogIO", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "640 AO-TriggerInputLine": "/Dev1/PFI0", "405 AO-TriggerInputLine": "/Dev1/PFI0", "640-Description": "DA device that is used as a shutter", "445 Shutter-TriggerSampleRate": "100000", "470 Shutter-TriggerSequenceLength": "1000", "COM1-StopBits": "1", "520 Shutter-SupportsTriggering": "0", "405-State": "0", "Core-Initialize": "1", "XLIGHT Emission Wheel-State": "3", "520 AO-Description": "NI DAC", "528 AO-TriggerInputLine": "/Dev1/PFI0", "445 Shutter-MaxVolts": "5.0000", "COM9-Handshaking": "Off", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "XLIGHT Spin motor-Label": "On (spinning)", "PRIME-PP 5 ENABLED": "No", "UUID": "c451982c-35a3-4c58-8df1-6a162a46c7a4", "PRIME-TriggerMode": "Internal Trigger", "COM1-Handshaking": "Off", "PRIME-Timing-PostTriggerDelayNs": "49344000.0000", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "Focus-Position": "17741.5000", "528 Shutter-Block voltage": "false", "Channel": "Il-G", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "555 Shutter-Demo": "No", "405 Shutter-Demo": "No", "528 AO-Name": "AnalogIO", "520-State": "1", "Core-Galvo": "", "405 Shutter-TriggerSampleRate": "100000", "Focus-FocusStepSize_(nm)": "100", "PRIME-PixelType": "16bit", "COM4-DataBits": "8", "470 AO-IOChannel": "Dev1/ao2", "520 AO-MinVolts": "0.0000", "COM9-Parity": "None", "PRIME-CircularBufferEnabled": "ON", "PRIME-PP 6 Reset": "No", "640 AO-Description": "NI DAC", "Source": "PRIME", "445 AO-MinVolts": "0.0000", "PIEZO-Name": "Stage", "445 AO-Description": "NI DAC", "PRIME-CircularBufferFrameCount": "46", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "520 AO-Volts": "4.5000", "528 Shutter-TriggerSampleRate": "100000", "XPositionUm": 19731.9, "OpticalPath-State": "4", "PRIME-PP 3 THRESHOLD": "80", "PRIME-PVCAM Version": "3.7.1", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Gain": "1-HDR", "640 Shutter-TriggerSequenceLength": "1000", "528 AO-SupportsTriggering": "0", "445 AO-TriggerInputLine": "/Dev1/PFI0", "OpticalPath-Name": "OpticalPath", "PRIME-PP 4 ENABLED": "Yes", "PRIME-PVCAM Adapter Version": "1.3.29", "COM3-Name": "COM3", "405 AO-MaxVolts": "5.0000", "520 Shutter-Block voltage": "false", "PRIME-Timing-ClearingTimeNs": "49344000.0000", "Core-Focus": "PIEZO", "528 AO-Block voltage": "false", "TE2000-Description": "Nikon TE2000 hub", "528 AO-MinVolts": "0.0000", "COM4-Parity": "None", "Summary": { "Slices": 11, "ChContrastMin": [ 0, 0, 0, 0, 0, 0 ], "PixelAspect": 1, "SlicesFirst": true, "Interval_ms": 0, "Time": "2019-12-18 21:36:42 -0500", "Prefix": "seq_4", "InitialPositionList": [ { "Label": "Pos0", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.21], "Focus": [17741.5], "XYStage": [ 44542.200000000004, 31636.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos1", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.22], "Focus": [17741.4], "XYStage": [ 43801.100000000006, 31637.9 ] }, "GridColumnIndex": 0 }, { "Label": "Pos2", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.5], "XYStage": [ 38561.3, 32040.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos3", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.185], "Focus": [17741.3], "XYStage": [ 37698.4, 32041.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos4", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.16], "Focus": [17741.5], "XYStage": [ 32375.4, 31758.9 ] }, "GridColumnIndex": 0 }, { "Label": "Pos5", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.17000000000002], "Focus": [17741.3], "XYStage": [ 31770.7, 31758.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos6", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.4], "XYStage": [ 26536.800000000003, 31068.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos7", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.205], "Focus": [17741.4], "XYStage": [ 25420.2, 31068.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos8", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.85], "Focus": [17741.3], "XYStage": [ 21059, 31508 ] }, "GridColumnIndex": 0 }, { "Label": "Pos9", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.82], "Focus": [17741.3], "XYStage": [ 19731.9, 31506.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos10", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.79], "Focus": [17741.4], "XYStage": [ 14528.5, 32007.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos11", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.83], "Focus": [17741.2], "XYStage": [ 13830.300000000001, 32009.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos12", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.79], "Focus": [17741.3], "XYStage": [ 8775.300000000001, 32017.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos13", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.815], "Focus": [17741.3], "XYStage": [ 8109.200000000001, 32008.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos14", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.805], "Focus": [17741.3], "XYStage": [ 3483.3, 31908.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos15", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.82], "Focus": [17741.2], "XYStage": [ 2686.8, 31903.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos16", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [211.48000000000002], "Focus": [17741.1], "XYStage": [ -1978.9, 31973.600000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos17", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [211.51500000000001], "Focus": [17741.1], "XYStage": [ -2957.8, 31982.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos18", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.195], "Focus": [17741.5], "XYStage": [ 47747.4, -576.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos19", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.23000000000002], "Focus": [17741.6], "XYStage": [ 47053.9, -576.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos20", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.205], "Focus": [17741.4], "XYStage": [ 41871.200000000004, -479.8 ] }, "GridColumnIndex": 0 }, { "Label": "Pos21", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.4], "XYStage": [ 40882.600000000006, -392 ] }, "GridColumnIndex": 0 }, { "Label": "Pos22", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.24], "Focus": [17741.4], "XYStage": [ 35905.6, -135.3 ] }, "GridColumnIndex": 0 }, { "Label": "Pos23", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.195], "Focus": [17741.5], "XYStage": [ 34990.9, -132.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos24", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.46], "Focus": [17741.4], "XYStage": [ 29678.100000000002, -958.1 ] }, "GridColumnIndex": 0 }, { "Label": "Pos25", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.44], "Focus": [17741.5], "XYStage": [ 28983.600000000002, -939.4000000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos26", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.475], "Focus": [17741.5], "XYStage": [ 23019.7, -122 ] }, "GridColumnIndex": 0 }, { "Label": "Pos27", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.44], "Focus": [17741.7], "XYStage": [ 22428, -116.80000000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos28", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.46], "Focus": [17741.7], "XYStage": [ 16364.900000000001, -181.10000000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos29", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.48000000000002], "Focus": [17741.5], "XYStage": [ 15712.1, -180 ] }, "GridColumnIndex": 0 }, { "Label": "Pos30", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.45000000000002], "Focus": [17741.7], "XYStage": [ 10346.1, 77.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos31", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.49], "Focus": [17741.4], "XYStage": [ 9755.5, 85.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos32", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.475], "Focus": [17741.5], "XYStage": [ 4775.5, 293.90000000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos33", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.445], "Focus": [17741.4], "XYStage": [ 3954.9, 293.90000000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos34", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.455], "Focus": [17741.4], "XYStage": [ -1324.9, 545.6 ] }, "GridColumnIndex": 0 }, { "Label": "Pos35", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.445], "Focus": [17741.3], "XYStage": [ -2097, 576.6 ] }, "GridColumnIndex": 0 } ], "z-step_um": 10, "GridRow": 0, "BitDepth": 16, "Depth": 2, "Channels": 6, "Source": "Micro-Manager", "ChColors": [ -16711681, -256, -65281, -1, -1, -16776961 ], "MetadataVersion": 10, "KeepShutterOpenChannels": true, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536, 65536 ], "CustomIntervals_ms": [], "Height": 1200, "MicroManagerVersion": "1.4.23 20180219", "GridColumn": 0, "UUID": "14a13bf7-c944-4672-b873-fcfbbb7ea13d", "PixelType": "GRAY16", "Width": 1200, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\Yan\\20191218-YC113-S7S8-miseq", "Frames": 1, "Comment": "", "UserName": "Nikon", "PixelSize_um": 1.1, "ROI": [ 0, 0, 1200, 1200 ], "Date": "2019-12-18", "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC", "P-DAPI-high" ], "KeepShutterOpenSlices": true, "IJType": 1, "ComputerName": "ZadorConfocal2", "Positions": 36, "PositionIndex": 9, "CameraTimeout": 20000, "TimeFirst": false }, "640 AO-Name": "AnalogIO", "Vincent-D3-Command": "Close" }, "FrameKey-0-0-6": { "COM4-StopBits": "1", "528 AO-MaxVolts": "5.0000", "XLIGHT Excitation Wheel-State": "1", "470 Shutter-Name": "AnalogIO", "Core-Shutter": "520", "555 AO-Demo": "No", "XLIGHT Dichroic-State": "2", "XYStage-StepSize-Y": "0.1000", "ZPositionUm": 258.82, "XYStage-StepSize-X": "0.1000", "PRIME-FirmwareVersion": "20.28", "PRIME-ClearCycles": "2", "520-Name": "DA Shutter", "405 Shutter-Name": "AnalogIO", "PIEZO-StepSize": "0.0050", "PRIME-ClearMode": "Pre-Exposure", "470 AO-MinVolts": "0.0000", "405 AO-SupportsTriggering": "1", "CameraChannelIndex": 0, "XYStage-StartSpeed": "500.0000", "COM9-StopBits": "2", "405 AO-Description": "NI DAC", "COM4-DelayBetweenCharsMs": "0.0000", "445 AO-Block voltage": "false", "520 AO-TriggerInputLine": "/Dev1/PFI0", "555 AO-Description": "NI DAC", "520 AO-TriggerSampleRate": "100000", "COM4-Verbose": "1", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM4-Name": "COM4", "405 Shutter-Volts": "0.0000", "Vincent-D3-Port": "COM1", "PRIME-PP 4 MIN ADU AFFECTED": "200", "470 Shutter-MinVolts": "0.0000", "PRIME-PP 3 ENABLED": "Yes", "470 AO-Description": "NI DAC", "PRIME-Description": "PVCAM API device adapter", "520 Shutter-MaxVolts": "5.0000", "520 Shutter-Volts": "5.0000", "528 AO-Sequenceable": "1", "PRIME-Binning": "1x1", "555 Shutter-MaxVolts": "5.0000", "528 Shutter-TriggerSequenceLength": "1000", "405 Shutter-Block voltage": "false", "XLIGHT Spin motor-State": "1", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "Vincent-D3-Time to open (ms)": "35.0000", "Vincent-D3-Time to close (ms)": "35.0000", "445 AO-TriggerSampleRate": "100000", "640 Shutter-IOChannel": "Dev1/ao14", "555 AO-Sequenceable": "1", "520 AO-Name": "AnalogIO", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "470 AO-Block voltage": "false", "COM1-Verbose": "1", "COM9-AnswerTimeout": "10000.0000", "528 Shutter-Name": "AnalogIO", "PRIME-Trigger Timeout (secs)": "10", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "NextFrame": 0, "470 AO-TriggerSequenceLength": "1000", "PRIME-TransposeXY": "0", "445 AO-TriggerSequenceLength": "1000", "COM1-Name": "COM1", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "COM9-DataBits": "8", "COM1-AnswerTimeout": "500.0000", "Camera": "", "TE2000-Version": "V2.50-V2.50-V2.10", "528 Shutter-Volts": "0.0000", "XLIGHT Hub-Name": "XLIGHT Hub", "PIEZO-Description": "Ludl stage driver adapter", "PositionName": "Pos9", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSampleRate": "100000", "PRIME-FanSpeedSetpoint": "High", "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "405 AO-TriggerSampleRate": "100000", "470 Shutter-Block voltage": "false", "470-State": "0", "COM3-Parity": "None", "405 AO-Volts": "0.0000", "PRIME-TransposeMirrorY": "0", "PRIME-ChipName": "GS144BSI", "470 AO-Volts": "0.0000", "PRIME-TransposeMirrorX": "0", "445 Shutter-SupportsTriggering": "0", "470 Shutter-SupportsTriggering": "0", "XLIGHT Hub-Description": "XLIGHT hub", "PRIME-PreampOffLimit": "10000", "470 AO-Sequenceable": "1", "528 Shutter-MaxVolts": "5.0000", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "Core-XYStage": "XYStage", "470 AO-TriggerSampleRate": "100000", "PRIME-Timing-PreTriggerDelayNs": "24672000.0000", "528-DA Device": "528 Shutter", "Focus-Description": "Nikon TE2000 focus adapter", "FilterBlock-State": "5", "520 AO-Demo": "No", "COM3-DelayBetweenCharsMs": "0.0000", "470 Shutter-Volts": "0.0000", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "PRIME-PP 2 THRESHOLD": "125", "PRIME-PP 4 THRESHOLD": "75", "XYStage-Acceleration": "4.0000", "470 Shutter-Demo": "No", "PRIME-Name": "PMPCIECam00", "640-DA Device": "640 Shutter", "405 AO-Block voltage": "false", "ElapsedTime-ms": 377786, "PRIME-Offset": "100", "445 AO-IOChannel": "Dev1/ao1", "640 AO-MaxVolts": "5.0000", "PositionIndex": 9, "PRIME-PP 1 ENABLED": "Yes", "COM1-BaudRate": "9600", "640-State": "0", "COM3-Verbose": "1", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "LudlController-TransmissionDelay": "0", "PRIME-FullWellCapacity": "62000", "PRIME-SMARTStreamingEnabled": "No", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "640 Shutter-Description": "NI DAC", "528-Description": "DA device that is used as a shutter", "COM4-Description": "Serial port driver (boost:asio)", "405 Shutter-Description": "NI DAC", "TE2000-Name": "TE2000", "528 Shutter-Description": "NI DAC", "COM9-Description": "Serial port driver (boost:asio)", "LudlController-Name": "LudlController", "445 Shutter-TriggerSequenceLength": "1000", "PRIME-SerialNumber": "A18B203008", "445 AO-Volts": "0.0000", "PRIME-X-dimension": "1200", "PRIME-ShutterCloseDelay": "0", "XLIGHT Disk slider-State": "2", "Position": "Default", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 AO-IOChannel": "Dev1/ao5", "520 AO-IOChannel": "Dev1/ao4", "528 AO-TriggerSequenceLength": "1000", "405 Shutter-MaxVolts": "5.0000", "470-DA Device": "470 Shutter", "PRIME-FTCapable": "No", "Core-ChannelGroup": "Channels", "555 AO-SupportsTriggering": "0", "PRIME-Y-dimension": "1200", "Frame": 0, "555 Shutter-Sequenceable": "1", "PRIME-Timing-ExposureTimeNs": "499998000.0000", "Core-ImageProcessor": "", "528-State": "0", "520 AO-Block voltage": "false", "XLIGHT Excitation Wheel-Label": "CFP/YFP", "640 Shutter-Demo": "No", "COM3-StopBits": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "520 Shutter-IOChannel": "Dev1/ao11", "Width": 1200, "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-SupportsTriggering": "0", "640 AO-TriggerSampleRate": "100000", "520-Description": "DA device that is used as a shutter", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "405 Shutter-Sequenceable": "1", "405 Shutter-SupportsTriggering": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-TriggerSampleRate": "100000", "640 Shutter-MaxVolts": "5.0000", "555 AO-MaxVolts": "5.0000", "520 AO-TriggerSequenceLength": "1000", "COM3-BaudRate": "9600", "COM9-BaudRate": "9600", "Vincent-D3-Channel #": "Ch. #1", "PRIME-PMode": "Normal", "OpticalPath-Label": "Left(100)", "BitDepth": 16, "555 AO-TriggerSequenceLength": "1000", "445 Shutter-Description": "NI DAC", "405 AO-Sequenceable": "1", "PRIME-AcquisitionMethod": "Callbacks", "555 AO-Name": "AnalogIO", "PRIME-ShutterMode": "Pre-Exposure", "COM3-Handshaking": "Off", "445 AO-SupportsTriggering": "0", "ROI": "0-0-1200-1200", "COM9-Verbose": "1", "PRIME-Color": "OFF", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "PRIME-ShutterOpenDelay": "0", "COM1-Parity": "None", "Slice": 6, "SlicePosition": 10, "COM9-DelayBetweenCharsMs": "0.0000", "555-Name": "DA Shutter", "PIEZO-LudlSingleAxisName": "B", "XLIGHT Emission Wheel-Label": "565/24(G)", "640 Shutter-Name": "AnalogIO", "640 AO-Sequenceable": "1", "520 Shutter-Demo": "No", "528 Shutter-Demo": "No", "470-Name": "DA Shutter", "520-DA Device": "520 Shutter", "XYStage-Description": "Ludl XY stage driver adapter", "445 Shutter-MinVolts": "0.0000", "528 AO-TriggerSampleRate": "100000", "405 AO-MinVolts": "0.0000", "405 Shutter-TriggerSequenceLength": "1000", "Height": 1200, "555 AO-Block voltage": "false", "640 Shutter-Volts": "0.0000", "PixelType": "GRAY16", "445 Shutter-Block voltage": "false", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "520 Shutter-TriggerSequenceLength": "1000", "COM9-Name": "COM9", "Core-SLM": "", "555 AO-Volts": "0.0000", "555 Shutter-Block voltage": "false", "XYStage-Speed": "1800.0000", "520 AO-MaxVolts": "5.0000", "528 Shutter-SupportsTriggering": "0", "COM1-Description": "Serial port driver (boost:asio)", "PRIME-MetadataEnabled": "No", "520 Shutter-MinVolts": "0.0000", "470 Shutter-Sequenceable": "1", "PRIME-PreampDelay": "0", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-MinVolts": "0.0000", "ChannelIndex": 0, "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Core-AutoShutter": "1", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "Exposure-ms": 320, "PRIME-PP 2 ENABLED": "Yes", "640 Shutter-TriggerSampleRate": "100000", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "470 Shutter-IOChannel": "Dev1/ao10", "405 AO-Demo": "No", "PixelSizeUm": 1.1, "470 AO-Demo": "No", "470 Shutter-MaxVolts": "5.0000", "Vincent-D3-Name": "Vincent-D3", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FileName": "img_000000000_Il-G_006.tif", "LudlController-Reset": "Operate", "PRIME-Exposure": "320.00", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "445-Name": "DA Shutter", "555 Shutter-Name": "AnalogIO", "520 Shutter-TriggerSampleRate": "100000", "445 Shutter-IOChannel": "Dev1/ao9", "PRIME-TransposeCorrection": "0", "XYStage-StepSize": "0.1000", "445-Description": "DA device that is used as a shutter", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 AO-IOChannel": "Dev1/ao7", "520 Shutter-Name": "AnalogIO", "COM3-DataBits": "8", "640 AO-Volts": "0.0000", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "555 Shutter-IOChannel": "Dev1/ao13", "WaitInterval": null, "520 Shutter-Sequenceable": "1", "YPositionUm": 31506.2, "445-State": "0", "445-DA Device": "445 Shutter", "PRIME-CircularBufferAutoSize": "ON", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "555 AO-TriggerSampleRate": "100000", "445 Shutter-Volts": "0.0000", "XLIGHT Hub-Port": "COM3", "Core-AutoFocus": "", "COM4-Handshaking": "Off", "528 Shutter-MinVolts": "0.0000", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "445 Shutter-Name": "AnalogIO", "528 Shutter-Sequenceable": "1", "XYStage-TransposeMirrorX": "0", "XYStage-TransposeMirrorY": "0", "PRIME-ReadoutRate": "100MHz 16bit", "XYStage-Name": "Stage", "640 AO-SupportsTriggering": "0", "640-Name": "DA Shutter", "640 Shutter-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "640 AO-Block voltage": "false", "445 Shutter-Sequenceable": "1", "470 AO-MaxVolts": "5.0000", "COM4-BaudRate": "9600", "470-Description": "DA device that is used as a shutter", "520 Shutter-Description": "NI DAC", "PRIME-CCDTemperature": "-14.8500", "Time": "2019-12-18 21:36:45 -0500", "640 AO-TriggerSequenceLength": "1000", "470 AO-Name": "AnalogIO", "XLIGHT Disk slider-Label": "Disk Pos 40um", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "Binning": "1x1", "TE2000-ModelType": "1", "COM3-AnswerTimeout": "500.0000", "555 Shutter-SupportsTriggering": "0", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "TE2000-Port": "COM4", "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Description": "NI DAC", "COM4-AnswerTimeout": "500.0000", "COM1-DelayBetweenCharsMs": "0.0000", "FilterBlock-Label": "Confocal", "445 AO-Name": "AnalogIO", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-Timing-ReadoutTimeNs": "24672000.0000", "528 AO-Demo": "No", "520 AO-Sequenceable": "1", "555-Description": "DA device that is used as a shutter", "405-DA Device": "405 Shutter", "405 AO-TriggerSequenceLength": "1000", "640 AO-MinVolts": "0.0000", "640 Shutter-Block voltage": "false", "FrameIndex": 0, "PRIME-Port": "CMOS", "555 AO-MinVolts": "0.0000", "COM3-Description": "Serial port driver (boost:asio)", "528 AO-Description": "NI DAC", "555 AO-IOChannel": "Dev1/ao6", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "555 AO-TriggerInputLine": "/Dev1/PFI0", "PRIME-ActualInterval-ms": "390.5200", "LudlController-Port": "COM9", "445 Shutter-Demo": "No", "PRIME-Trigger-Expose Out-Mux": "1", "SliceIndex": 6, "PRIME-CCDTemperatureSetPoint": "-15.0000", "405 Shutter-MinVolts": "0.0000", "PRIME-ExposeOutMode": "First Row", "AxisPositions": null, "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Volts": "0.0000", "528-Name": "DA Shutter", "555-State": "0", "XLIGHT Dichroic-Label": "CFP/YFP", "445 AO-Sequenceable": "1", "405-Name": "DA Shutter", "405 AO-IOChannel": "Dev1/ao0", "Core-Camera": "PRIME", "Focus-Name": "Focus", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "405 Shutter-IOChannel": "Dev1/ao8", "640 AO-Demo": "No", "640 Shutter-SupportsTriggering": "0", "445 AO-Demo": "No", "555-DA Device": "555 Shutter", "470 Shutter-Description": "NI DAC", "528 AO-Volts": "0.0000", "COM1-DataBits": "8", "Vincent-D3-Address": "x", "520 AO-SupportsTriggering": "0", "405-Description": "DA device that is used as a shutter", "640 Shutter-MinVolts": "0.0000", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "405 AO-Name": "AnalogIO", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "640 AO-TriggerInputLine": "/Dev1/PFI0", "405 AO-TriggerInputLine": "/Dev1/PFI0", "640-Description": "DA device that is used as a shutter", "445 Shutter-TriggerSampleRate": "100000", "470 Shutter-TriggerSequenceLength": "1000", "COM1-StopBits": "1", "520 Shutter-SupportsTriggering": "0", "405-State": "0", "Core-Initialize": "1", "XLIGHT Emission Wheel-State": "3", "520 AO-Description": "NI DAC", "528 AO-TriggerInputLine": "/Dev1/PFI0", "445 Shutter-MaxVolts": "5.0000", "COM9-Handshaking": "Off", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "XLIGHT Spin motor-Label": "On (spinning)", "PRIME-PP 5 ENABLED": "No", "UUID": "88b144b5-d644-48c0-9933-4afdb591d14d", "PRIME-TriggerMode": "Internal Trigger", "COM1-Handshaking": "Off", "PRIME-Timing-PostTriggerDelayNs": "49344000.0000", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "Focus-Position": "17741.5000", "528 Shutter-Block voltage": "false", "Channel": "Il-G", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "555 Shutter-Demo": "No", "405 Shutter-Demo": "No", "528 AO-Name": "AnalogIO", "520-State": "1", "Core-Galvo": "", "405 Shutter-TriggerSampleRate": "100000", "Focus-FocusStepSize_(nm)": "100", "PRIME-PixelType": "16bit", "COM4-DataBits": "8", "470 AO-IOChannel": "Dev1/ao2", "520 AO-MinVolts": "0.0000", "COM9-Parity": "None", "PRIME-CircularBufferEnabled": "ON", "PRIME-PP 6 Reset": "No", "640 AO-Description": "NI DAC", "Source": "PRIME", "445 AO-MinVolts": "0.0000", "PIEZO-Name": "Stage", "445 AO-Description": "NI DAC", "PRIME-CircularBufferFrameCount": "46", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "520 AO-Volts": "4.5000", "528 Shutter-TriggerSampleRate": "100000", "XPositionUm": 19731.9, "OpticalPath-State": "4", "PRIME-PP 3 THRESHOLD": "80", "PRIME-PVCAM Version": "3.7.1", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Gain": "1-HDR", "640 Shutter-TriggerSequenceLength": "1000", "528 AO-SupportsTriggering": "0", "445 AO-TriggerInputLine": "/Dev1/PFI0", "OpticalPath-Name": "OpticalPath", "PRIME-PP 4 ENABLED": "Yes", "PRIME-PVCAM Adapter Version": "1.3.29", "COM3-Name": "COM3", "405 AO-MaxVolts": "5.0000", "520 Shutter-Block voltage": "false", "PRIME-Timing-ClearingTimeNs": "49344000.0000", "Core-Focus": "PIEZO", "528 AO-Block voltage": "false", "TE2000-Description": "Nikon TE2000 hub", "528 AO-MinVolts": "0.0000", "COM4-Parity": "None", "Summary": { "Slices": 11, "ChContrastMin": [ 0, 0, 0, 0, 0, 0 ], "PixelAspect": 1, "SlicesFirst": true, "Interval_ms": 0, "Time": "2019-12-18 21:36:42 -0500", "Prefix": "seq_4", "InitialPositionList": [ { "Label": "Pos0", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.21], "Focus": [17741.5], "XYStage": [ 44542.200000000004, 31636.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos1", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.22], "Focus": [17741.4], "XYStage": [ 43801.100000000006, 31637.9 ] }, "GridColumnIndex": 0 }, { "Label": "Pos2", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.5], "XYStage": [ 38561.3, 32040.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos3", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.185], "Focus": [17741.3], "XYStage": [ 37698.4, 32041.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos4", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.16], "Focus": [17741.5], "XYStage": [ 32375.4, 31758.9 ] }, "GridColumnIndex": 0 }, { "Label": "Pos5", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.17000000000002], "Focus": [17741.3], "XYStage": [ 31770.7, 31758.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos6", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.4], "XYStage": [ 26536.800000000003, 31068.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos7", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.205], "Focus": [17741.4], "XYStage": [ 25420.2, 31068.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos8", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.85], "Focus": [17741.3], "XYStage": [ 21059, 31508 ] }, "GridColumnIndex": 0 }, { "Label": "Pos9", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.82], "Focus": [17741.3], "XYStage": [ 19731.9, 31506.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos10", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.79], "Focus": [17741.4], "XYStage": [ 14528.5, 32007.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos11", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.83], "Focus": [17741.2], "XYStage": [ 13830.300000000001, 32009.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos12", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.79], "Focus": [17741.3], "XYStage": [ 8775.300000000001, 32017.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos13", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.815], "Focus": [17741.3], "XYStage": [ 8109.200000000001, 32008.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos14", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.805], "Focus": [17741.3], "XYStage": [ 3483.3, 31908.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos15", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.82], "Focus": [17741.2], "XYStage": [ 2686.8, 31903.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos16", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [211.48000000000002], "Focus": [17741.1], "XYStage": [ -1978.9, 31973.600000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos17", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [211.51500000000001], "Focus": [17741.1], "XYStage": [ -2957.8, 31982.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos18", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.195], "Focus": [17741.5], "XYStage": [ 47747.4, -576.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos19", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.23000000000002], "Focus": [17741.6], "XYStage": [ 47053.9, -576.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos20", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.205], "Focus": [17741.4], "XYStage": [ 41871.200000000004, -479.8 ] }, "GridColumnIndex": 0 }, { "Label": "Pos21", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.4], "XYStage": [ 40882.600000000006, -392 ] }, "GridColumnIndex": 0 }, { "Label": "Pos22", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.24], "Focus": [17741.4], "XYStage": [ 35905.6, -135.3 ] }, "GridColumnIndex": 0 }, { "Label": "Pos23", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.195], "Focus": [17741.5], "XYStage": [ 34990.9, -132.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos24", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.46], "Focus": [17741.4], "XYStage": [ 29678.100000000002, -958.1 ] }, "GridColumnIndex": 0 }, { "Label": "Pos25", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.44], "Focus": [17741.5], "XYStage": [ 28983.600000000002, -939.4000000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos26", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.475], "Focus": [17741.5], "XYStage": [ 23019.7, -122 ] }, "GridColumnIndex": 0 }, { "Label": "Pos27", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.44], "Focus": [17741.7], "XYStage": [ 22428, -116.80000000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos28", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.46], "Focus": [17741.7], "XYStage": [ 16364.900000000001, -181.10000000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos29", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.48000000000002], "Focus": [17741.5], "XYStage": [ 15712.1, -180 ] }, "GridColumnIndex": 0 }, { "Label": "Pos30", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.45000000000002], "Focus": [17741.7], "XYStage": [ 10346.1, 77.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos31", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.49], "Focus": [17741.4], "XYStage": [ 9755.5, 85.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos32", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.475], "Focus": [17741.5], "XYStage": [ 4775.5, 293.90000000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos33", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.445], "Focus": [17741.4], "XYStage": [ 3954.9, 293.90000000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos34", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.455], "Focus": [17741.4], "XYStage": [ -1324.9, 545.6 ] }, "GridColumnIndex": 0 }, { "Label": "Pos35", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.445], "Focus": [17741.3], "XYStage": [ -2097, 576.6 ] }, "GridColumnIndex": 0 } ], "z-step_um": 10, "GridRow": 0, "BitDepth": 16, "Depth": 2, "Channels": 6, "Source": "Micro-Manager", "ChColors": [ -16711681, -256, -65281, -1, -1, -16776961 ], "MetadataVersion": 10, "KeepShutterOpenChannels": true, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536, 65536 ], "CustomIntervals_ms": [], "Height": 1200, "MicroManagerVersion": "1.4.23 20180219", "GridColumn": 0, "UUID": "14a13bf7-c944-4672-b873-fcfbbb7ea13d", "PixelType": "GRAY16", "Width": 1200, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\Yan\\20191218-YC113-S7S8-miseq", "Frames": 1, "Comment": "", "UserName": "Nikon", "PixelSize_um": 1.1, "ROI": [ 0, 0, 1200, 1200 ], "Date": "2019-12-18", "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC", "P-DAPI-high" ], "KeepShutterOpenSlices": true, "IJType": 1, "ComputerName": "ZadorConfocal2", "Positions": 36, "PositionIndex": 9, "CameraTimeout": 20000, "TimeFirst": false }, "640 AO-Name": "AnalogIO", "Vincent-D3-Command": "Close" }, "FrameKey-0-0-7": { "COM4-StopBits": "1", "528 AO-MaxVolts": "5.0000", "XLIGHT Excitation Wheel-State": "1", "470 Shutter-Name": "AnalogIO", "Core-Shutter": "520", "555 AO-Demo": "No", "XLIGHT Dichroic-State": "2", "XYStage-StepSize-Y": "0.1000", "ZPositionUm": 268.82, "XYStage-StepSize-X": "0.1000", "PRIME-FirmwareVersion": "20.28", "PRIME-ClearCycles": "2", "520-Name": "DA Shutter", "405 Shutter-Name": "AnalogIO", "PIEZO-StepSize": "0.0050", "PRIME-ClearMode": "Pre-Exposure", "470 AO-MinVolts": "0.0000", "405 AO-SupportsTriggering": "1", "CameraChannelIndex": 0, "XYStage-StartSpeed": "500.0000", "COM9-StopBits": "2", "405 AO-Description": "NI DAC", "COM4-DelayBetweenCharsMs": "0.0000", "445 AO-Block voltage": "false", "520 AO-TriggerInputLine": "/Dev1/PFI0", "555 AO-Description": "NI DAC", "520 AO-TriggerSampleRate": "100000", "COM4-Verbose": "1", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM4-Name": "COM4", "405 Shutter-Volts": "0.0000", "Vincent-D3-Port": "COM1", "PRIME-PP 4 MIN ADU AFFECTED": "200", "470 Shutter-MinVolts": "0.0000", "PRIME-PP 3 ENABLED": "Yes", "470 AO-Description": "NI DAC", "PRIME-Description": "PVCAM API device adapter", "520 Shutter-MaxVolts": "5.0000", "520 Shutter-Volts": "5.0000", "528 AO-Sequenceable": "1", "PRIME-Binning": "1x1", "555 Shutter-MaxVolts": "5.0000", "528 Shutter-TriggerSequenceLength": "1000", "405 Shutter-Block voltage": "false", "XLIGHT Spin motor-State": "1", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "Vincent-D3-Time to open (ms)": "35.0000", "Vincent-D3-Time to close (ms)": "35.0000", "445 AO-TriggerSampleRate": "100000", "640 Shutter-IOChannel": "Dev1/ao14", "555 AO-Sequenceable": "1", "520 AO-Name": "AnalogIO", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "470 AO-Block voltage": "false", "COM1-Verbose": "1", "COM9-AnswerTimeout": "10000.0000", "528 Shutter-Name": "AnalogIO", "PRIME-Trigger Timeout (secs)": "10", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "NextFrame": 0, "470 AO-TriggerSequenceLength": "1000", "PRIME-TransposeXY": "0", "445 AO-TriggerSequenceLength": "1000", "COM1-Name": "COM1", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "COM9-DataBits": "8", "COM1-AnswerTimeout": "500.0000", "Camera": "", "TE2000-Version": "V2.50-V2.50-V2.10", "528 Shutter-Volts": "0.0000", "XLIGHT Hub-Name": "XLIGHT Hub", "PIEZO-Description": "Ludl stage driver adapter", "PositionName": "Pos9", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSampleRate": "100000", "PRIME-FanSpeedSetpoint": "High", "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "405 AO-TriggerSampleRate": "100000", "470 Shutter-Block voltage": "false", "470-State": "0", "COM3-Parity": "None", "405 AO-Volts": "0.0000", "PRIME-TransposeMirrorY": "0", "PRIME-ChipName": "GS144BSI", "470 AO-Volts": "0.0000", "PRIME-TransposeMirrorX": "0", "445 Shutter-SupportsTriggering": "0", "470 Shutter-SupportsTriggering": "0", "XLIGHT Hub-Description": "XLIGHT hub", "PRIME-PreampOffLimit": "10000", "470 AO-Sequenceable": "1", "528 Shutter-MaxVolts": "5.0000", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "Core-XYStage": "XYStage", "470 AO-TriggerSampleRate": "100000", "PRIME-Timing-PreTriggerDelayNs": "24672000.0000", "528-DA Device": "528 Shutter", "Focus-Description": "Nikon TE2000 focus adapter", "FilterBlock-State": "5", "520 AO-Demo": "No", "COM3-DelayBetweenCharsMs": "0.0000", "470 Shutter-Volts": "0.0000", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "PRIME-PP 2 THRESHOLD": "125", "PRIME-PP 4 THRESHOLD": "75", "XYStage-Acceleration": "4.0000", "470 Shutter-Demo": "No", "PRIME-Name": "PMPCIECam00", "640-DA Device": "640 Shutter", "405 AO-Block voltage": "false", "ElapsedTime-ms": 378352, "PRIME-Offset": "100", "445 AO-IOChannel": "Dev1/ao1", "640 AO-MaxVolts": "5.0000", "PositionIndex": 9, "PRIME-PP 1 ENABLED": "Yes", "COM1-BaudRate": "9600", "640-State": "0", "COM3-Verbose": "1", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "LudlController-TransmissionDelay": "0", "PRIME-FullWellCapacity": "62000", "PRIME-SMARTStreamingEnabled": "No", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "640 Shutter-Description": "NI DAC", "528-Description": "DA device that is used as a shutter", "COM4-Description": "Serial port driver (boost:asio)", "405 Shutter-Description": "NI DAC", "TE2000-Name": "TE2000", "528 Shutter-Description": "NI DAC", "COM9-Description": "Serial port driver (boost:asio)", "LudlController-Name": "LudlController", "445 Shutter-TriggerSequenceLength": "1000", "PRIME-SerialNumber": "A18B203008", "445 AO-Volts": "0.0000", "PRIME-X-dimension": "1200", "PRIME-ShutterCloseDelay": "0", "XLIGHT Disk slider-State": "2", "Position": "Default", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 AO-IOChannel": "Dev1/ao5", "520 AO-IOChannel": "Dev1/ao4", "528 AO-TriggerSequenceLength": "1000", "405 Shutter-MaxVolts": "5.0000", "470-DA Device": "470 Shutter", "PRIME-FTCapable": "No", "Core-ChannelGroup": "Channels", "555 AO-SupportsTriggering": "0", "PRIME-Y-dimension": "1200", "Frame": 0, "555 Shutter-Sequenceable": "1", "PRIME-Timing-ExposureTimeNs": "499998000.0000", "Core-ImageProcessor": "", "528-State": "0", "520 AO-Block voltage": "false", "XLIGHT Excitation Wheel-Label": "CFP/YFP", "640 Shutter-Demo": "No", "COM3-StopBits": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "520 Shutter-IOChannel": "Dev1/ao11", "Width": 1200, "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-SupportsTriggering": "0", "640 AO-TriggerSampleRate": "100000", "520-Description": "DA device that is used as a shutter", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "405 Shutter-Sequenceable": "1", "405 Shutter-SupportsTriggering": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-TriggerSampleRate": "100000", "640 Shutter-MaxVolts": "5.0000", "555 AO-MaxVolts": "5.0000", "520 AO-TriggerSequenceLength": "1000", "COM3-BaudRate": "9600", "COM9-BaudRate": "9600", "Vincent-D3-Channel #": "Ch. #1", "PRIME-PMode": "Normal", "OpticalPath-Label": "Left(100)", "BitDepth": 16, "555 AO-TriggerSequenceLength": "1000", "445 Shutter-Description": "NI DAC", "405 AO-Sequenceable": "1", "PRIME-AcquisitionMethod": "Callbacks", "555 AO-Name": "AnalogIO", "PRIME-ShutterMode": "Pre-Exposure", "COM3-Handshaking": "Off", "445 AO-SupportsTriggering": "0", "ROI": "0-0-1200-1200", "COM9-Verbose": "1", "PRIME-Color": "OFF", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "PRIME-ShutterOpenDelay": "0", "COM1-Parity": "None", "Slice": 7, "SlicePosition": 20, "COM9-DelayBetweenCharsMs": "0.0000", "555-Name": "DA Shutter", "PIEZO-LudlSingleAxisName": "B", "XLIGHT Emission Wheel-Label": "565/24(G)", "640 Shutter-Name": "AnalogIO", "640 AO-Sequenceable": "1", "520 Shutter-Demo": "No", "528 Shutter-Demo": "No", "470-Name": "DA Shutter", "520-DA Device": "520 Shutter", "XYStage-Description": "Ludl XY stage driver adapter", "445 Shutter-MinVolts": "0.0000", "528 AO-TriggerSampleRate": "100000", "405 AO-MinVolts": "0.0000", "405 Shutter-TriggerSequenceLength": "1000", "Height": 1200, "555 AO-Block voltage": "false", "640 Shutter-Volts": "0.0000", "PixelType": "GRAY16", "445 Shutter-Block voltage": "false", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "520 Shutter-TriggerSequenceLength": "1000", "COM9-Name": "COM9", "Core-SLM": "", "555 AO-Volts": "0.0000", "555 Shutter-Block voltage": "false", "XYStage-Speed": "1800.0000", "520 AO-MaxVolts": "5.0000", "528 Shutter-SupportsTriggering": "0", "COM1-Description": "Serial port driver (boost:asio)", "PRIME-MetadataEnabled": "No", "520 Shutter-MinVolts": "0.0000", "470 Shutter-Sequenceable": "1", "PRIME-PreampDelay": "0", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-MinVolts": "0.0000", "ChannelIndex": 0, "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Core-AutoShutter": "1", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "Exposure-ms": 320, "PRIME-PP 2 ENABLED": "Yes", "640 Shutter-TriggerSampleRate": "100000", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "470 Shutter-IOChannel": "Dev1/ao10", "405 AO-Demo": "No", "PixelSizeUm": 1.1, "470 AO-Demo": "No", "470 Shutter-MaxVolts": "5.0000", "Vincent-D3-Name": "Vincent-D3", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FileName": "img_000000000_Il-G_007.tif", "LudlController-Reset": "Operate", "PRIME-Exposure": "320.00", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "445-Name": "DA Shutter", "555 Shutter-Name": "AnalogIO", "520 Shutter-TriggerSampleRate": "100000", "445 Shutter-IOChannel": "Dev1/ao9", "PRIME-TransposeCorrection": "0", "XYStage-StepSize": "0.1000", "445-Description": "DA device that is used as a shutter", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 AO-IOChannel": "Dev1/ao7", "520 Shutter-Name": "AnalogIO", "COM3-DataBits": "8", "640 AO-Volts": "0.0000", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "555 Shutter-IOChannel": "Dev1/ao13", "WaitInterval": null, "520 Shutter-Sequenceable": "1", "YPositionUm": 31506.2, "445-State": "0", "445-DA Device": "445 Shutter", "PRIME-CircularBufferAutoSize": "ON", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "555 AO-TriggerSampleRate": "100000", "445 Shutter-Volts": "0.0000", "XLIGHT Hub-Port": "COM3", "Core-AutoFocus": "", "COM4-Handshaking": "Off", "528 Shutter-MinVolts": "0.0000", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "445 Shutter-Name": "AnalogIO", "528 Shutter-Sequenceable": "1", "XYStage-TransposeMirrorX": "0", "XYStage-TransposeMirrorY": "0", "PRIME-ReadoutRate": "100MHz 16bit", "XYStage-Name": "Stage", "640 AO-SupportsTriggering": "0", "640-Name": "DA Shutter", "640 Shutter-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "640 AO-Block voltage": "false", "445 Shutter-Sequenceable": "1", "470 AO-MaxVolts": "5.0000", "COM4-BaudRate": "9600", "470-Description": "DA device that is used as a shutter", "520 Shutter-Description": "NI DAC", "PRIME-CCDTemperature": "-14.8500", "Time": "2019-12-18 21:36:46 -0500", "640 AO-TriggerSequenceLength": "1000", "470 AO-Name": "AnalogIO", "XLIGHT Disk slider-Label": "Disk Pos 40um", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "Binning": "1x1", "TE2000-ModelType": "1", "COM3-AnswerTimeout": "500.0000", "555 Shutter-SupportsTriggering": "0", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "TE2000-Port": "COM4", "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Description": "NI DAC", "COM4-AnswerTimeout": "500.0000", "COM1-DelayBetweenCharsMs": "0.0000", "FilterBlock-Label": "Confocal", "445 AO-Name": "AnalogIO", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-Timing-ReadoutTimeNs": "24672000.0000", "528 AO-Demo": "No", "520 AO-Sequenceable": "1", "555-Description": "DA device that is used as a shutter", "405-DA Device": "405 Shutter", "405 AO-TriggerSequenceLength": "1000", "640 AO-MinVolts": "0.0000", "640 Shutter-Block voltage": "false", "FrameIndex": 0, "PRIME-Port": "CMOS", "555 AO-MinVolts": "0.0000", "COM3-Description": "Serial port driver (boost:asio)", "528 AO-Description": "NI DAC", "555 AO-IOChannel": "Dev1/ao6", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "555 AO-TriggerInputLine": "/Dev1/PFI0", "PRIME-ActualInterval-ms": "390.5200", "LudlController-Port": "COM9", "445 Shutter-Demo": "No", "PRIME-Trigger-Expose Out-Mux": "1", "SliceIndex": 7, "PRIME-CCDTemperatureSetPoint": "-15.0000", "405 Shutter-MinVolts": "0.0000", "PRIME-ExposeOutMode": "First Row", "AxisPositions": null, "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Volts": "0.0000", "528-Name": "DA Shutter", "555-State": "0", "XLIGHT Dichroic-Label": "CFP/YFP", "445 AO-Sequenceable": "1", "405-Name": "DA Shutter", "405 AO-IOChannel": "Dev1/ao0", "Core-Camera": "PRIME", "Focus-Name": "Focus", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "405 Shutter-IOChannel": "Dev1/ao8", "640 AO-Demo": "No", "640 Shutter-SupportsTriggering": "0", "445 AO-Demo": "No", "555-DA Device": "555 Shutter", "470 Shutter-Description": "NI DAC", "528 AO-Volts": "0.0000", "COM1-DataBits": "8", "Vincent-D3-Address": "x", "520 AO-SupportsTriggering": "0", "405-Description": "DA device that is used as a shutter", "640 Shutter-MinVolts": "0.0000", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "405 AO-Name": "AnalogIO", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "640 AO-TriggerInputLine": "/Dev1/PFI0", "405 AO-TriggerInputLine": "/Dev1/PFI0", "640-Description": "DA device that is used as a shutter", "445 Shutter-TriggerSampleRate": "100000", "470 Shutter-TriggerSequenceLength": "1000", "COM1-StopBits": "1", "520 Shutter-SupportsTriggering": "0", "405-State": "0", "Core-Initialize": "1", "XLIGHT Emission Wheel-State": "3", "520 AO-Description": "NI DAC", "528 AO-TriggerInputLine": "/Dev1/PFI0", "445 Shutter-MaxVolts": "5.0000", "COM9-Handshaking": "Off", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "XLIGHT Spin motor-Label": "On (spinning)", "PRIME-PP 5 ENABLED": "No", "UUID": "c44dc53d-d530-4c9b-ba1d-464501844e60", "PRIME-TriggerMode": "Internal Trigger", "COM1-Handshaking": "Off", "PRIME-Timing-PostTriggerDelayNs": "49344000.0000", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "Focus-Position": "17741.5000", "528 Shutter-Block voltage": "false", "Channel": "Il-G", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "555 Shutter-Demo": "No", "405 Shutter-Demo": "No", "528 AO-Name": "AnalogIO", "520-State": "1", "Core-Galvo": "", "405 Shutter-TriggerSampleRate": "100000", "Focus-FocusStepSize_(nm)": "100", "PRIME-PixelType": "16bit", "COM4-DataBits": "8", "470 AO-IOChannel": "Dev1/ao2", "520 AO-MinVolts": "0.0000", "COM9-Parity": "None", "PRIME-CircularBufferEnabled": "ON", "PRIME-PP 6 Reset": "No", "640 AO-Description": "NI DAC", "Source": "PRIME", "445 AO-MinVolts": "0.0000", "PIEZO-Name": "Stage", "445 AO-Description": "NI DAC", "PRIME-CircularBufferFrameCount": "46", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "520 AO-Volts": "4.5000", "528 Shutter-TriggerSampleRate": "100000", "XPositionUm": 19731.9, "OpticalPath-State": "4", "PRIME-PP 3 THRESHOLD": "80", "PRIME-PVCAM Version": "3.7.1", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Gain": "1-HDR", "640 Shutter-TriggerSequenceLength": "1000", "528 AO-SupportsTriggering": "0", "445 AO-TriggerInputLine": "/Dev1/PFI0", "OpticalPath-Name": "OpticalPath", "PRIME-PP 4 ENABLED": "Yes", "PRIME-PVCAM Adapter Version": "1.3.29", "COM3-Name": "COM3", "405 AO-MaxVolts": "5.0000", "520 Shutter-Block voltage": "false", "PRIME-Timing-ClearingTimeNs": "49344000.0000", "Core-Focus": "PIEZO", "528 AO-Block voltage": "false", "TE2000-Description": "Nikon TE2000 hub", "528 AO-MinVolts": "0.0000", "COM4-Parity": "None", "Summary": { "Slices": 11, "ChContrastMin": [ 0, 0, 0, 0, 0, 0 ], "PixelAspect": 1, "SlicesFirst": true, "Interval_ms": 0, "Time": "2019-12-18 21:36:42 -0500", "Prefix": "seq_4", "InitialPositionList": [ { "Label": "Pos0", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.21], "Focus": [17741.5], "XYStage": [ 44542.200000000004, 31636.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos1", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.22], "Focus": [17741.4], "XYStage": [ 43801.100000000006, 31637.9 ] }, "GridColumnIndex": 0 }, { "Label": "Pos2", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.5], "XYStage": [ 38561.3, 32040.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos3", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.185], "Focus": [17741.3], "XYStage": [ 37698.4, 32041.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos4", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.16], "Focus": [17741.5], "XYStage": [ 32375.4, 31758.9 ] }, "GridColumnIndex": 0 }, { "Label": "Pos5", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.17000000000002], "Focus": [17741.3], "XYStage": [ 31770.7, 31758.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos6", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.4], "XYStage": [ 26536.800000000003, 31068.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos7", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.205], "Focus": [17741.4], "XYStage": [ 25420.2, 31068.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos8", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.85], "Focus": [17741.3], "XYStage": [ 21059, 31508 ] }, "GridColumnIndex": 0 }, { "Label": "Pos9", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.82], "Focus": [17741.3], "XYStage": [ 19731.9, 31506.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos10", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.79], "Focus": [17741.4], "XYStage": [ 14528.5, 32007.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos11", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.83], "Focus": [17741.2], "XYStage": [ 13830.300000000001, 32009.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos12", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.79], "Focus": [17741.3], "XYStage": [ 8775.300000000001, 32017.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos13", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.815], "Focus": [17741.3], "XYStage": [ 8109.200000000001, 32008.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos14", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.805], "Focus": [17741.3], "XYStage": [ 3483.3, 31908.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos15", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.82], "Focus": [17741.2], "XYStage": [ 2686.8, 31903.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos16", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [211.48000000000002], "Focus": [17741.1], "XYStage": [ -1978.9, 31973.600000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos17", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [211.51500000000001], "Focus": [17741.1], "XYStage": [ -2957.8, 31982.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos18", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.195], "Focus": [17741.5], "XYStage": [ 47747.4, -576.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos19", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.23000000000002], "Focus": [17741.6], "XYStage": [ 47053.9, -576.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos20", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.205], "Focus": [17741.4], "XYStage": [ 41871.200000000004, -479.8 ] }, "GridColumnIndex": 0 }, { "Label": "Pos21", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.4], "XYStage": [ 40882.600000000006, -392 ] }, "GridColumnIndex": 0 }, { "Label": "Pos22", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.24], "Focus": [17741.4], "XYStage": [ 35905.6, -135.3 ] }, "GridColumnIndex": 0 }, { "Label": "Pos23", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.195], "Focus": [17741.5], "XYStage": [ 34990.9, -132.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos24", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.46], "Focus": [17741.4], "XYStage": [ 29678.100000000002, -958.1 ] }, "GridColumnIndex": 0 }, { "Label": "Pos25", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.44], "Focus": [17741.5], "XYStage": [ 28983.600000000002, -939.4000000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos26", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.475], "Focus": [17741.5], "XYStage": [ 23019.7, -122 ] }, "GridColumnIndex": 0 }, { "Label": "Pos27", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.44], "Focus": [17741.7], "XYStage": [ 22428, -116.80000000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos28", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.46], "Focus": [17741.7], "XYStage": [ 16364.900000000001, -181.10000000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos29", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.48000000000002], "Focus": [17741.5], "XYStage": [ 15712.1, -180 ] }, "GridColumnIndex": 0 }, { "Label": "Pos30", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.45000000000002], "Focus": [17741.7], "XYStage": [ 10346.1, 77.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos31", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.49], "Focus": [17741.4], "XYStage": [ 9755.5, 85.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos32", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.475], "Focus": [17741.5], "XYStage": [ 4775.5, 293.90000000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos33", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.445], "Focus": [17741.4], "XYStage": [ 3954.9, 293.90000000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos34", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.455], "Focus": [17741.4], "XYStage": [ -1324.9, 545.6 ] }, "GridColumnIndex": 0 }, { "Label": "Pos35", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.445], "Focus": [17741.3], "XYStage": [ -2097, 576.6 ] }, "GridColumnIndex": 0 } ], "z-step_um": 10, "GridRow": 0, "BitDepth": 16, "Depth": 2, "Channels": 6, "Source": "Micro-Manager", "ChColors": [ -16711681, -256, -65281, -1, -1, -16776961 ], "MetadataVersion": 10, "KeepShutterOpenChannels": true, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536, 65536 ], "CustomIntervals_ms": [], "Height": 1200, "MicroManagerVersion": "1.4.23 20180219", "GridColumn": 0, "UUID": "14a13bf7-c944-4672-b873-fcfbbb7ea13d", "PixelType": "GRAY16", "Width": 1200, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\Yan\\20191218-YC113-S7S8-miseq", "Frames": 1, "Comment": "", "UserName": "Nikon", "PixelSize_um": 1.1, "ROI": [ 0, 0, 1200, 1200 ], "Date": "2019-12-18", "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC", "P-DAPI-high" ], "KeepShutterOpenSlices": true, "IJType": 1, "ComputerName": "ZadorConfocal2", "Positions": 36, "PositionIndex": 9, "CameraTimeout": 20000, "TimeFirst": false }, "640 AO-Name": "AnalogIO", "Vincent-D3-Command": "Close" }, "FrameKey-0-0-8": { "COM4-StopBits": "1", "528 AO-MaxVolts": "5.0000", "XLIGHT Excitation Wheel-State": "1", "470 Shutter-Name": "AnalogIO", "Core-Shutter": "520", "555 AO-Demo": "No", "XLIGHT Dichroic-State": "2", "XYStage-StepSize-Y": "0.1000", "ZPositionUm": 278.82, "XYStage-StepSize-X": "0.1000", "PRIME-FirmwareVersion": "20.28", "PRIME-ClearCycles": "2", "520-Name": "DA Shutter", "405 Shutter-Name": "AnalogIO", "PIEZO-StepSize": "0.0050", "PRIME-ClearMode": "Pre-Exposure", "470 AO-MinVolts": "0.0000", "405 AO-SupportsTriggering": "1", "CameraChannelIndex": 0, "XYStage-StartSpeed": "500.0000", "COM9-StopBits": "2", "405 AO-Description": "NI DAC", "COM4-DelayBetweenCharsMs": "0.0000", "445 AO-Block voltage": "false", "520 AO-TriggerInputLine": "/Dev1/PFI0", "555 AO-Description": "NI DAC", "520 AO-TriggerSampleRate": "100000", "COM4-Verbose": "1", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM4-Name": "COM4", "405 Shutter-Volts": "0.0000", "Vincent-D3-Port": "COM1", "PRIME-PP 4 MIN ADU AFFECTED": "200", "470 Shutter-MinVolts": "0.0000", "PRIME-PP 3 ENABLED": "Yes", "470 AO-Description": "NI DAC", "PRIME-Description": "PVCAM API device adapter", "520 Shutter-MaxVolts": "5.0000", "520 Shutter-Volts": "5.0000", "528 AO-Sequenceable": "1", "PRIME-Binning": "1x1", "555 Shutter-MaxVolts": "5.0000", "528 Shutter-TriggerSequenceLength": "1000", "405 Shutter-Block voltage": "false", "XLIGHT Spin motor-State": "1", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "Vincent-D3-Time to open (ms)": "35.0000", "Vincent-D3-Time to close (ms)": "35.0000", "445 AO-TriggerSampleRate": "100000", "640 Shutter-IOChannel": "Dev1/ao14", "555 AO-Sequenceable": "1", "520 AO-Name": "AnalogIO", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "470 AO-Block voltage": "false", "COM1-Verbose": "1", "COM9-AnswerTimeout": "10000.0000", "528 Shutter-Name": "AnalogIO", "PRIME-Trigger Timeout (secs)": "10", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "NextFrame": 0, "470 AO-TriggerSequenceLength": "1000", "PRIME-TransposeXY": "0", "445 AO-TriggerSequenceLength": "1000", "COM1-Name": "COM1", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "COM9-DataBits": "8", "COM1-AnswerTimeout": "500.0000", "Camera": "", "TE2000-Version": "V2.50-V2.50-V2.10", "528 Shutter-Volts": "0.0000", "XLIGHT Hub-Name": "XLIGHT Hub", "PIEZO-Description": "Ludl stage driver adapter", "PositionName": "Pos9", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSampleRate": "100000", "PRIME-FanSpeedSetpoint": "High", "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "405 AO-TriggerSampleRate": "100000", "470 Shutter-Block voltage": "false", "470-State": "0", "COM3-Parity": "None", "405 AO-Volts": "0.0000", "PRIME-TransposeMirrorY": "0", "PRIME-ChipName": "GS144BSI", "470 AO-Volts": "0.0000", "PRIME-TransposeMirrorX": "0", "445 Shutter-SupportsTriggering": "0", "470 Shutter-SupportsTriggering": "0", "XLIGHT Hub-Description": "XLIGHT hub", "PRIME-PreampOffLimit": "10000", "470 AO-Sequenceable": "1", "528 Shutter-MaxVolts": "5.0000", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "Core-XYStage": "XYStage", "470 AO-TriggerSampleRate": "100000", "PRIME-Timing-PreTriggerDelayNs": "24672000.0000", "528-DA Device": "528 Shutter", "Focus-Description": "Nikon TE2000 focus adapter", "FilterBlock-State": "5", "520 AO-Demo": "No", "COM3-DelayBetweenCharsMs": "0.0000", "470 Shutter-Volts": "0.0000", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "PRIME-PP 2 THRESHOLD": "125", "PRIME-PP 4 THRESHOLD": "75", "XYStage-Acceleration": "4.0000", "470 Shutter-Demo": "No", "PRIME-Name": "PMPCIECam00", "640-DA Device": "640 Shutter", "405 AO-Block voltage": "false", "ElapsedTime-ms": 378957, "PRIME-Offset": "100", "445 AO-IOChannel": "Dev1/ao1", "640 AO-MaxVolts": "5.0000", "PositionIndex": 9, "PRIME-PP 1 ENABLED": "Yes", "COM1-BaudRate": "9600", "640-State": "0", "COM3-Verbose": "1", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "LudlController-TransmissionDelay": "0", "PRIME-FullWellCapacity": "62000", "PRIME-SMARTStreamingEnabled": "No", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "640 Shutter-Description": "NI DAC", "528-Description": "DA device that is used as a shutter", "COM4-Description": "Serial port driver (boost:asio)", "405 Shutter-Description": "NI DAC", "TE2000-Name": "TE2000", "528 Shutter-Description": "NI DAC", "COM9-Description": "Serial port driver (boost:asio)", "LudlController-Name": "LudlController", "445 Shutter-TriggerSequenceLength": "1000", "PRIME-SerialNumber": "A18B203008", "445 AO-Volts": "0.0000", "PRIME-X-dimension": "1200", "PRIME-ShutterCloseDelay": "0", "XLIGHT Disk slider-State": "2", "Position": "Default", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 AO-IOChannel": "Dev1/ao5", "520 AO-IOChannel": "Dev1/ao4", "528 AO-TriggerSequenceLength": "1000", "405 Shutter-MaxVolts": "5.0000", "470-DA Device": "470 Shutter", "PRIME-FTCapable": "No", "Core-ChannelGroup": "Channels", "555 AO-SupportsTriggering": "0", "PRIME-Y-dimension": "1200", "Frame": 0, "555 Shutter-Sequenceable": "1", "PRIME-Timing-ExposureTimeNs": "499998000.0000", "Core-ImageProcessor": "", "528-State": "0", "520 AO-Block voltage": "false", "XLIGHT Excitation Wheel-Label": "CFP/YFP", "640 Shutter-Demo": "No", "COM3-StopBits": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "520 Shutter-IOChannel": "Dev1/ao11", "Width": 1200, "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-SupportsTriggering": "0", "640 AO-TriggerSampleRate": "100000", "520-Description": "DA device that is used as a shutter", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "405 Shutter-Sequenceable": "1", "405 Shutter-SupportsTriggering": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-TriggerSampleRate": "100000", "640 Shutter-MaxVolts": "5.0000", "555 AO-MaxVolts": "5.0000", "520 AO-TriggerSequenceLength": "1000", "COM3-BaudRate": "9600", "COM9-BaudRate": "9600", "Vincent-D3-Channel #": "Ch. #1", "PRIME-PMode": "Normal", "OpticalPath-Label": "Left(100)", "BitDepth": 16, "555 AO-TriggerSequenceLength": "1000", "445 Shutter-Description": "NI DAC", "405 AO-Sequenceable": "1", "PRIME-AcquisitionMethod": "Callbacks", "555 AO-Name": "AnalogIO", "PRIME-ShutterMode": "Pre-Exposure", "COM3-Handshaking": "Off", "445 AO-SupportsTriggering": "0", "ROI": "0-0-1200-1200", "COM9-Verbose": "1", "PRIME-Color": "OFF", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "PRIME-ShutterOpenDelay": "0", "COM1-Parity": "None", "Slice": 8, "SlicePosition": 30, "COM9-DelayBetweenCharsMs": "0.0000", "555-Name": "DA Shutter", "PIEZO-LudlSingleAxisName": "B", "XLIGHT Emission Wheel-Label": "565/24(G)", "640 Shutter-Name": "AnalogIO", "640 AO-Sequenceable": "1", "520 Shutter-Demo": "No", "528 Shutter-Demo": "No", "470-Name": "DA Shutter", "520-DA Device": "520 Shutter", "XYStage-Description": "Ludl XY stage driver adapter", "445 Shutter-MinVolts": "0.0000", "528 AO-TriggerSampleRate": "100000", "405 AO-MinVolts": "0.0000", "405 Shutter-TriggerSequenceLength": "1000", "Height": 1200, "555 AO-Block voltage": "false", "640 Shutter-Volts": "0.0000", "PixelType": "GRAY16", "445 Shutter-Block voltage": "false", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "520 Shutter-TriggerSequenceLength": "1000", "COM9-Name": "COM9", "Core-SLM": "", "555 AO-Volts": "0.0000", "555 Shutter-Block voltage": "false", "XYStage-Speed": "1800.0000", "520 AO-MaxVolts": "5.0000", "528 Shutter-SupportsTriggering": "0", "COM1-Description": "Serial port driver (boost:asio)", "PRIME-MetadataEnabled": "No", "520 Shutter-MinVolts": "0.0000", "470 Shutter-Sequenceable": "1", "PRIME-PreampDelay": "0", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-MinVolts": "0.0000", "ChannelIndex": 0, "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Core-AutoShutter": "1", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "Exposure-ms": 320, "PRIME-PP 2 ENABLED": "Yes", "640 Shutter-TriggerSampleRate": "100000", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "470 Shutter-IOChannel": "Dev1/ao10", "405 AO-Demo": "No", "PixelSizeUm": 1.1, "470 AO-Demo": "No", "470 Shutter-MaxVolts": "5.0000", "Vincent-D3-Name": "Vincent-D3", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FileName": "img_000000000_Il-G_008.tif", "LudlController-Reset": "Operate", "PRIME-Exposure": "320.00", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "445-Name": "DA Shutter", "555 Shutter-Name": "AnalogIO", "520 Shutter-TriggerSampleRate": "100000", "445 Shutter-IOChannel": "Dev1/ao9", "PRIME-TransposeCorrection": "0", "XYStage-StepSize": "0.1000", "445-Description": "DA device that is used as a shutter", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 AO-IOChannel": "Dev1/ao7", "520 Shutter-Name": "AnalogIO", "COM3-DataBits": "8", "640 AO-Volts": "0.0000", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "555 Shutter-IOChannel": "Dev1/ao13", "WaitInterval": null, "520 Shutter-Sequenceable": "1", "YPositionUm": 31506.2, "445-State": "0", "445-DA Device": "445 Shutter", "PRIME-CircularBufferAutoSize": "ON", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "555 AO-TriggerSampleRate": "100000", "445 Shutter-Volts": "0.0000", "XLIGHT Hub-Port": "COM3", "Core-AutoFocus": "", "COM4-Handshaking": "Off", "528 Shutter-MinVolts": "0.0000", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "445 Shutter-Name": "AnalogIO", "528 Shutter-Sequenceable": "1", "XYStage-TransposeMirrorX": "0", "XYStage-TransposeMirrorY": "0", "PRIME-ReadoutRate": "100MHz 16bit", "XYStage-Name": "Stage", "640 AO-SupportsTriggering": "0", "640-Name": "DA Shutter", "640 Shutter-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "640 AO-Block voltage": "false", "445 Shutter-Sequenceable": "1", "470 AO-MaxVolts": "5.0000", "COM4-BaudRate": "9600", "470-Description": "DA device that is used as a shutter", "520 Shutter-Description": "NI DAC", "PRIME-CCDTemperature": "-14.8500", "Time": "2019-12-18 21:36:46 -0500", "640 AO-TriggerSequenceLength": "1000", "470 AO-Name": "AnalogIO", "XLIGHT Disk slider-Label": "Disk Pos 40um", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "Binning": "1x1", "TE2000-ModelType": "1", "COM3-AnswerTimeout": "500.0000", "555 Shutter-SupportsTriggering": "0", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "TE2000-Port": "COM4", "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Description": "NI DAC", "COM4-AnswerTimeout": "500.0000", "COM1-DelayBetweenCharsMs": "0.0000", "FilterBlock-Label": "Confocal", "445 AO-Name": "AnalogIO", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-Timing-ReadoutTimeNs": "24672000.0000", "528 AO-Demo": "No", "520 AO-Sequenceable": "1", "555-Description": "DA device that is used as a shutter", "405-DA Device": "405 Shutter", "405 AO-TriggerSequenceLength": "1000", "640 AO-MinVolts": "0.0000", "640 Shutter-Block voltage": "false", "FrameIndex": 0, "PRIME-Port": "CMOS", "555 AO-MinVolts": "0.0000", "COM3-Description": "Serial port driver (boost:asio)", "528 AO-Description": "NI DAC", "555 AO-IOChannel": "Dev1/ao6", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "555 AO-TriggerInputLine": "/Dev1/PFI0", "PRIME-ActualInterval-ms": "390.5200", "LudlController-Port": "COM9", "445 Shutter-Demo": "No", "PRIME-Trigger-Expose Out-Mux": "1", "SliceIndex": 8, "PRIME-CCDTemperatureSetPoint": "-15.0000", "405 Shutter-MinVolts": "0.0000", "PRIME-ExposeOutMode": "First Row", "AxisPositions": null, "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Volts": "0.0000", "528-Name": "DA Shutter", "555-State": "0", "XLIGHT Dichroic-Label": "CFP/YFP", "445 AO-Sequenceable": "1", "405-Name": "DA Shutter", "405 AO-IOChannel": "Dev1/ao0", "Core-Camera": "PRIME", "Focus-Name": "Focus", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "405 Shutter-IOChannel": "Dev1/ao8", "640 AO-Demo": "No", "640 Shutter-SupportsTriggering": "0", "445 AO-Demo": "No", "555-DA Device": "555 Shutter", "470 Shutter-Description": "NI DAC", "528 AO-Volts": "0.0000", "COM1-DataBits": "8", "Vincent-D3-Address": "x", "520 AO-SupportsTriggering": "0", "405-Description": "DA device that is used as a shutter", "640 Shutter-MinVolts": "0.0000", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "405 AO-Name": "AnalogIO", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "640 AO-TriggerInputLine": "/Dev1/PFI0", "405 AO-TriggerInputLine": "/Dev1/PFI0", "640-Description": "DA device that is used as a shutter", "445 Shutter-TriggerSampleRate": "100000", "470 Shutter-TriggerSequenceLength": "1000", "COM1-StopBits": "1", "520 Shutter-SupportsTriggering": "0", "405-State": "0", "Core-Initialize": "1", "XLIGHT Emission Wheel-State": "3", "520 AO-Description": "NI DAC", "528 AO-TriggerInputLine": "/Dev1/PFI0", "445 Shutter-MaxVolts": "5.0000", "COM9-Handshaking": "Off", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "XLIGHT Spin motor-Label": "On (spinning)", "PRIME-PP 5 ENABLED": "No", "UUID": "d5e0593c-1bc3-4019-b64e-2fc95ff359f3", "PRIME-TriggerMode": "Internal Trigger", "COM1-Handshaking": "Off", "PRIME-Timing-PostTriggerDelayNs": "49344000.0000", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "Focus-Position": "17741.5000", "528 Shutter-Block voltage": "false", "Channel": "Il-G", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "555 Shutter-Demo": "No", "405 Shutter-Demo": "No", "528 AO-Name": "AnalogIO", "520-State": "1", "Core-Galvo": "", "405 Shutter-TriggerSampleRate": "100000", "Focus-FocusStepSize_(nm)": "100", "PRIME-PixelType": "16bit", "COM4-DataBits": "8", "470 AO-IOChannel": "Dev1/ao2", "520 AO-MinVolts": "0.0000", "COM9-Parity": "None", "PRIME-CircularBufferEnabled": "ON", "PRIME-PP 6 Reset": "No", "640 AO-Description": "NI DAC", "Source": "PRIME", "445 AO-MinVolts": "0.0000", "PIEZO-Name": "Stage", "445 AO-Description": "NI DAC", "PRIME-CircularBufferFrameCount": "46", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "520 AO-Volts": "4.5000", "528 Shutter-TriggerSampleRate": "100000", "XPositionUm": 19731.9, "OpticalPath-State": "4", "PRIME-PP 3 THRESHOLD": "80", "PRIME-PVCAM Version": "3.7.1", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Gain": "1-HDR", "640 Shutter-TriggerSequenceLength": "1000", "528 AO-SupportsTriggering": "0", "445 AO-TriggerInputLine": "/Dev1/PFI0", "OpticalPath-Name": "OpticalPath", "PRIME-PP 4 ENABLED": "Yes", "PRIME-PVCAM Adapter Version": "1.3.29", "COM3-Name": "COM3", "405 AO-MaxVolts": "5.0000", "520 Shutter-Block voltage": "false", "PRIME-Timing-ClearingTimeNs": "49344000.0000", "Core-Focus": "PIEZO", "528 AO-Block voltage": "false", "TE2000-Description": "Nikon TE2000 hub", "528 AO-MinVolts": "0.0000", "COM4-Parity": "None", "Summary": { "Slices": 11, "ChContrastMin": [ 0, 0, 0, 0, 0, 0 ], "PixelAspect": 1, "SlicesFirst": true, "Interval_ms": 0, "Time": "2019-12-18 21:36:42 -0500", "Prefix": "seq_4", "InitialPositionList": [ { "Label": "Pos0", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.21], "Focus": [17741.5], "XYStage": [ 44542.200000000004, 31636.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos1", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.22], "Focus": [17741.4], "XYStage": [ 43801.100000000006, 31637.9 ] }, "GridColumnIndex": 0 }, { "Label": "Pos2", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.5], "XYStage": [ 38561.3, 32040.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos3", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.185], "Focus": [17741.3], "XYStage": [ 37698.4, 32041.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos4", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.16], "Focus": [17741.5], "XYStage": [ 32375.4, 31758.9 ] }, "GridColumnIndex": 0 }, { "Label": "Pos5", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.17000000000002], "Focus": [17741.3], "XYStage": [ 31770.7, 31758.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos6", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.4], "XYStage": [ 26536.800000000003, 31068.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos7", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.205], "Focus": [17741.4], "XYStage": [ 25420.2, 31068.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos8", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.85], "Focus": [17741.3], "XYStage": [ 21059, 31508 ] }, "GridColumnIndex": 0 }, { "Label": "Pos9", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.82], "Focus": [17741.3], "XYStage": [ 19731.9, 31506.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos10", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.79], "Focus": [17741.4], "XYStage": [ 14528.5, 32007.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos11", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.83], "Focus": [17741.2], "XYStage": [ 13830.300000000001, 32009.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos12", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.79], "Focus": [17741.3], "XYStage": [ 8775.300000000001, 32017.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos13", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.815], "Focus": [17741.3], "XYStage": [ 8109.200000000001, 32008.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos14", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.805], "Focus": [17741.3], "XYStage": [ 3483.3, 31908.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos15", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.82], "Focus": [17741.2], "XYStage": [ 2686.8, 31903.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos16", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [211.48000000000002], "Focus": [17741.1], "XYStage": [ -1978.9, 31973.600000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos17", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [211.51500000000001], "Focus": [17741.1], "XYStage": [ -2957.8, 31982.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos18", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.195], "Focus": [17741.5], "XYStage": [ 47747.4, -576.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos19", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.23000000000002], "Focus": [17741.6], "XYStage": [ 47053.9, -576.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos20", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.205], "Focus": [17741.4], "XYStage": [ 41871.200000000004, -479.8 ] }, "GridColumnIndex": 0 }, { "Label": "Pos21", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.4], "XYStage": [ 40882.600000000006, -392 ] }, "GridColumnIndex": 0 }, { "Label": "Pos22", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.24], "Focus": [17741.4], "XYStage": [ 35905.6, -135.3 ] }, "GridColumnIndex": 0 }, { "Label": "Pos23", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.195], "Focus": [17741.5], "XYStage": [ 34990.9, -132.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos24", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.46], "Focus": [17741.4], "XYStage": [ 29678.100000000002, -958.1 ] }, "GridColumnIndex": 0 }, { "Label": "Pos25", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.44], "Focus": [17741.5], "XYStage": [ 28983.600000000002, -939.4000000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos26", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.475], "Focus": [17741.5], "XYStage": [ 23019.7, -122 ] }, "GridColumnIndex": 0 }, { "Label": "Pos27", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.44], "Focus": [17741.7], "XYStage": [ 22428, -116.80000000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos28", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.46], "Focus": [17741.7], "XYStage": [ 16364.900000000001, -181.10000000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos29", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.48000000000002], "Focus": [17741.5], "XYStage": [ 15712.1, -180 ] }, "GridColumnIndex": 0 }, { "Label": "Pos30", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.45000000000002], "Focus": [17741.7], "XYStage": [ 10346.1, 77.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos31", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.49], "Focus": [17741.4], "XYStage": [ 9755.5, 85.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos32", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.475], "Focus": [17741.5], "XYStage": [ 4775.5, 293.90000000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos33", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.445], "Focus": [17741.4], "XYStage": [ 3954.9, 293.90000000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos34", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.455], "Focus": [17741.4], "XYStage": [ -1324.9, 545.6 ] }, "GridColumnIndex": 0 }, { "Label": "Pos35", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.445], "Focus": [17741.3], "XYStage": [ -2097, 576.6 ] }, "GridColumnIndex": 0 } ], "z-step_um": 10, "GridRow": 0, "BitDepth": 16, "Depth": 2, "Channels": 6, "Source": "Micro-Manager", "ChColors": [ -16711681, -256, -65281, -1, -1, -16776961 ], "MetadataVersion": 10, "KeepShutterOpenChannels": true, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536, 65536 ], "CustomIntervals_ms": [], "Height": 1200, "MicroManagerVersion": "1.4.23 20180219", "GridColumn": 0, "UUID": "14a13bf7-c944-4672-b873-fcfbbb7ea13d", "PixelType": "GRAY16", "Width": 1200, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\Yan\\20191218-YC113-S7S8-miseq", "Frames": 1, "Comment": "", "UserName": "Nikon", "PixelSize_um": 1.1, "ROI": [ 0, 0, 1200, 1200 ], "Date": "2019-12-18", "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC", "P-DAPI-high" ], "KeepShutterOpenSlices": true, "IJType": 1, "ComputerName": "ZadorConfocal2", "Positions": 36, "PositionIndex": 9, "CameraTimeout": 20000, "TimeFirst": false }, "640 AO-Name": "AnalogIO", "Vincent-D3-Command": "Close" }, "FrameKey-0-0-9": { "COM4-StopBits": "1", "528 AO-MaxVolts": "5.0000", "XLIGHT Excitation Wheel-State": "1", "470 Shutter-Name": "AnalogIO", "Core-Shutter": "520", "555 AO-Demo": "No", "XLIGHT Dichroic-State": "2", "XYStage-StepSize-Y": "0.1000", "ZPositionUm": 288.82, "XYStage-StepSize-X": "0.1000", "PRIME-FirmwareVersion": "20.28", "PRIME-ClearCycles": "2", "520-Name": "DA Shutter", "405 Shutter-Name": "AnalogIO", "PIEZO-StepSize": "0.0050", "PRIME-ClearMode": "Pre-Exposure", "470 AO-MinVolts": "0.0000", "405 AO-SupportsTriggering": "1", "CameraChannelIndex": 0, "XYStage-StartSpeed": "500.0000", "COM9-StopBits": "2", "405 AO-Description": "NI DAC", "COM4-DelayBetweenCharsMs": "0.0000", "445 AO-Block voltage": "false", "520 AO-TriggerInputLine": "/Dev1/PFI0", "555 AO-Description": "NI DAC", "520 AO-TriggerSampleRate": "100000", "COM4-Verbose": "1", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM4-Name": "COM4", "405 Shutter-Volts": "0.0000", "Vincent-D3-Port": "COM1", "PRIME-PP 4 MIN ADU AFFECTED": "200", "470 Shutter-MinVolts": "0.0000", "PRIME-PP 3 ENABLED": "Yes", "470 AO-Description": "NI DAC", "PRIME-Description": "PVCAM API device adapter", "520 Shutter-MaxVolts": "5.0000", "520 Shutter-Volts": "5.0000", "528 AO-Sequenceable": "1", "PRIME-Binning": "1x1", "555 Shutter-MaxVolts": "5.0000", "528 Shutter-TriggerSequenceLength": "1000", "405 Shutter-Block voltage": "false", "XLIGHT Spin motor-State": "1", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "Vincent-D3-Time to open (ms)": "35.0000", "Vincent-D3-Time to close (ms)": "35.0000", "445 AO-TriggerSampleRate": "100000", "640 Shutter-IOChannel": "Dev1/ao14", "555 AO-Sequenceable": "1", "520 AO-Name": "AnalogIO", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "470 AO-Block voltage": "false", "COM1-Verbose": "1", "COM9-AnswerTimeout": "10000.0000", "528 Shutter-Name": "AnalogIO", "PRIME-Trigger Timeout (secs)": "10", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "NextFrame": 0, "470 AO-TriggerSequenceLength": "1000", "PRIME-TransposeXY": "0", "445 AO-TriggerSequenceLength": "1000", "COM1-Name": "COM1", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "COM9-DataBits": "8", "COM1-AnswerTimeout": "500.0000", "Camera": "", "TE2000-Version": "V2.50-V2.50-V2.10", "528 Shutter-Volts": "0.0000", "XLIGHT Hub-Name": "XLIGHT Hub", "PIEZO-Description": "Ludl stage driver adapter", "PositionName": "Pos9", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSampleRate": "100000", "PRIME-FanSpeedSetpoint": "High", "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "405 AO-TriggerSampleRate": "100000", "470 Shutter-Block voltage": "false", "470-State": "0", "COM3-Parity": "None", "405 AO-Volts": "0.0000", "PRIME-TransposeMirrorY": "0", "PRIME-ChipName": "GS144BSI", "470 AO-Volts": "0.0000", "PRIME-TransposeMirrorX": "0", "445 Shutter-SupportsTriggering": "0", "470 Shutter-SupportsTriggering": "0", "XLIGHT Hub-Description": "XLIGHT hub", "PRIME-PreampOffLimit": "10000", "470 AO-Sequenceable": "1", "528 Shutter-MaxVolts": "5.0000", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "Core-XYStage": "XYStage", "470 AO-TriggerSampleRate": "100000", "PRIME-Timing-PreTriggerDelayNs": "24672000.0000", "528-DA Device": "528 Shutter", "Focus-Description": "Nikon TE2000 focus adapter", "FilterBlock-State": "5", "520 AO-Demo": "No", "COM3-DelayBetweenCharsMs": "0.0000", "470 Shutter-Volts": "0.0000", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "PRIME-PP 2 THRESHOLD": "125", "PRIME-PP 4 THRESHOLD": "75", "XYStage-Acceleration": "4.0000", "470 Shutter-Demo": "No", "PRIME-Name": "PMPCIECam00", "640-DA Device": "640 Shutter", "405 AO-Block voltage": "false", "ElapsedTime-ms": 379515, "PRIME-Offset": "100", "445 AO-IOChannel": "Dev1/ao1", "640 AO-MaxVolts": "5.0000", "PositionIndex": 9, "PRIME-PP 1 ENABLED": "Yes", "COM1-BaudRate": "9600", "640-State": "0", "COM3-Verbose": "1", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "LudlController-TransmissionDelay": "0", "PRIME-FullWellCapacity": "62000", "PRIME-SMARTStreamingEnabled": "No", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "640 Shutter-Description": "NI DAC", "528-Description": "DA device that is used as a shutter", "COM4-Description": "Serial port driver (boost:asio)", "405 Shutter-Description": "NI DAC", "TE2000-Name": "TE2000", "528 Shutter-Description": "NI DAC", "COM9-Description": "Serial port driver (boost:asio)", "LudlController-Name": "LudlController", "445 Shutter-TriggerSequenceLength": "1000", "PRIME-SerialNumber": "A18B203008", "445 AO-Volts": "0.0000", "PRIME-X-dimension": "1200", "PRIME-ShutterCloseDelay": "0", "XLIGHT Disk slider-State": "2", "Position": "Default", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 AO-IOChannel": "Dev1/ao5", "520 AO-IOChannel": "Dev1/ao4", "528 AO-TriggerSequenceLength": "1000", "405 Shutter-MaxVolts": "5.0000", "470-DA Device": "470 Shutter", "PRIME-FTCapable": "No", "Core-ChannelGroup": "Channels", "555 AO-SupportsTriggering": "0", "PRIME-Y-dimension": "1200", "Frame": 0, "555 Shutter-Sequenceable": "1", "PRIME-Timing-ExposureTimeNs": "499998000.0000", "Core-ImageProcessor": "", "528-State": "0", "520 AO-Block voltage": "false", "XLIGHT Excitation Wheel-Label": "CFP/YFP", "640 Shutter-Demo": "No", "COM3-StopBits": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "520 Shutter-IOChannel": "Dev1/ao11", "Width": 1200, "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-SupportsTriggering": "0", "640 AO-TriggerSampleRate": "100000", "520-Description": "DA device that is used as a shutter", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "405 Shutter-Sequenceable": "1", "405 Shutter-SupportsTriggering": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-TriggerSampleRate": "100000", "640 Shutter-MaxVolts": "5.0000", "555 AO-MaxVolts": "5.0000", "520 AO-TriggerSequenceLength": "1000", "COM3-BaudRate": "9600", "COM9-BaudRate": "9600", "Vincent-D3-Channel #": "Ch. #1", "PRIME-PMode": "Normal", "OpticalPath-Label": "Left(100)", "BitDepth": 16, "555 AO-TriggerSequenceLength": "1000", "445 Shutter-Description": "NI DAC", "405 AO-Sequenceable": "1", "PRIME-AcquisitionMethod": "Callbacks", "555 AO-Name": "AnalogIO", "PRIME-ShutterMode": "Pre-Exposure", "COM3-Handshaking": "Off", "445 AO-SupportsTriggering": "0", "ROI": "0-0-1200-1200", "COM9-Verbose": "1", "PRIME-Color": "OFF", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "PRIME-ShutterOpenDelay": "0", "COM1-Parity": "None", "Slice": 9, "SlicePosition": 40, "COM9-DelayBetweenCharsMs": "0.0000", "555-Name": "DA Shutter", "PIEZO-LudlSingleAxisName": "B", "XLIGHT Emission Wheel-Label": "565/24(G)", "640 Shutter-Name": "AnalogIO", "640 AO-Sequenceable": "1", "520 Shutter-Demo": "No", "528 Shutter-Demo": "No", "470-Name": "DA Shutter", "520-DA Device": "520 Shutter", "XYStage-Description": "Ludl XY stage driver adapter", "445 Shutter-MinVolts": "0.0000", "528 AO-TriggerSampleRate": "100000", "405 AO-MinVolts": "0.0000", "405 Shutter-TriggerSequenceLength": "1000", "Height": 1200, "555 AO-Block voltage": "false", "640 Shutter-Volts": "0.0000", "PixelType": "GRAY16", "445 Shutter-Block voltage": "false", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "520 Shutter-TriggerSequenceLength": "1000", "COM9-Name": "COM9", "Core-SLM": "", "555 AO-Volts": "0.0000", "555 Shutter-Block voltage": "false", "XYStage-Speed": "1800.0000", "520 AO-MaxVolts": "5.0000", "528 Shutter-SupportsTriggering": "0", "COM1-Description": "Serial port driver (boost:asio)", "PRIME-MetadataEnabled": "No", "520 Shutter-MinVolts": "0.0000", "470 Shutter-Sequenceable": "1", "PRIME-PreampDelay": "0", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-MinVolts": "0.0000", "ChannelIndex": 0, "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Core-AutoShutter": "1", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "Exposure-ms": 320, "PRIME-PP 2 ENABLED": "Yes", "640 Shutter-TriggerSampleRate": "100000", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "470 Shutter-IOChannel": "Dev1/ao10", "405 AO-Demo": "No", "PixelSizeUm": 1.1, "470 AO-Demo": "No", "470 Shutter-MaxVolts": "5.0000", "Vincent-D3-Name": "Vincent-D3", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FileName": "img_000000000_Il-G_009.tif", "LudlController-Reset": "Operate", "PRIME-Exposure": "320.00", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "445-Name": "DA Shutter", "555 Shutter-Name": "AnalogIO", "520 Shutter-TriggerSampleRate": "100000", "445 Shutter-IOChannel": "Dev1/ao9", "PRIME-TransposeCorrection": "0", "XYStage-StepSize": "0.1000", "445-Description": "DA device that is used as a shutter", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 AO-IOChannel": "Dev1/ao7", "520 Shutter-Name": "AnalogIO", "COM3-DataBits": "8", "640 AO-Volts": "0.0000", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "555 Shutter-IOChannel": "Dev1/ao13", "WaitInterval": null, "520 Shutter-Sequenceable": "1", "YPositionUm": 31506.2, "445-State": "0", "445-DA Device": "445 Shutter", "PRIME-CircularBufferAutoSize": "ON", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "555 AO-TriggerSampleRate": "100000", "445 Shutter-Volts": "0.0000", "XLIGHT Hub-Port": "COM3", "Core-AutoFocus": "", "COM4-Handshaking": "Off", "528 Shutter-MinVolts": "0.0000", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "445 Shutter-Name": "AnalogIO", "528 Shutter-Sequenceable": "1", "XYStage-TransposeMirrorX": "0", "XYStage-TransposeMirrorY": "0", "PRIME-ReadoutRate": "100MHz 16bit", "XYStage-Name": "Stage", "640 AO-SupportsTriggering": "0", "640-Name": "DA Shutter", "640 Shutter-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "640 AO-Block voltage": "false", "445 Shutter-Sequenceable": "1", "470 AO-MaxVolts": "5.0000", "COM4-BaudRate": "9600", "470-Description": "DA device that is used as a shutter", "520 Shutter-Description": "NI DAC", "PRIME-CCDTemperature": "-14.8500", "Time": "2019-12-18 21:36:47 -0500", "640 AO-TriggerSequenceLength": "1000", "470 AO-Name": "AnalogIO", "XLIGHT Disk slider-Label": "Disk Pos 40um", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "Binning": "1x1", "TE2000-ModelType": "1", "COM3-AnswerTimeout": "500.0000", "555 Shutter-SupportsTriggering": "0", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "TE2000-Port": "COM4", "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Description": "NI DAC", "COM4-AnswerTimeout": "500.0000", "COM1-DelayBetweenCharsMs": "0.0000", "FilterBlock-Label": "Confocal", "445 AO-Name": "AnalogIO", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-Timing-ReadoutTimeNs": "24672000.0000", "528 AO-Demo": "No", "520 AO-Sequenceable": "1", "555-Description": "DA device that is used as a shutter", "405-DA Device": "405 Shutter", "405 AO-TriggerSequenceLength": "1000", "640 AO-MinVolts": "0.0000", "640 Shutter-Block voltage": "false", "FrameIndex": 0, "PRIME-Port": "CMOS", "555 AO-MinVolts": "0.0000", "COM3-Description": "Serial port driver (boost:asio)", "528 AO-Description": "NI DAC", "555 AO-IOChannel": "Dev1/ao6", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "555 AO-TriggerInputLine": "/Dev1/PFI0", "PRIME-ActualInterval-ms": "390.5200", "LudlController-Port": "COM9", "445 Shutter-Demo": "No", "PRIME-Trigger-Expose Out-Mux": "1", "SliceIndex": 9, "PRIME-CCDTemperatureSetPoint": "-15.0000", "405 Shutter-MinVolts": "0.0000", "PRIME-ExposeOutMode": "First Row", "AxisPositions": null, "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Volts": "0.0000", "528-Name": "DA Shutter", "555-State": "0", "XLIGHT Dichroic-Label": "CFP/YFP", "445 AO-Sequenceable": "1", "405-Name": "DA Shutter", "405 AO-IOChannel": "Dev1/ao0", "Core-Camera": "PRIME", "Focus-Name": "Focus", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "405 Shutter-IOChannel": "Dev1/ao8", "640 AO-Demo": "No", "640 Shutter-SupportsTriggering": "0", "445 AO-Demo": "No", "555-DA Device": "555 Shutter", "470 Shutter-Description": "NI DAC", "528 AO-Volts": "0.0000", "COM1-DataBits": "8", "Vincent-D3-Address": "x", "520 AO-SupportsTriggering": "0", "405-Description": "DA device that is used as a shutter", "640 Shutter-MinVolts": "0.0000", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "405 AO-Name": "AnalogIO", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "640 AO-TriggerInputLine": "/Dev1/PFI0", "405 AO-TriggerInputLine": "/Dev1/PFI0", "640-Description": "DA device that is used as a shutter", "445 Shutter-TriggerSampleRate": "100000", "470 Shutter-TriggerSequenceLength": "1000", "COM1-StopBits": "1", "520 Shutter-SupportsTriggering": "0", "405-State": "0", "Core-Initialize": "1", "XLIGHT Emission Wheel-State": "3", "520 AO-Description": "NI DAC", "528 AO-TriggerInputLine": "/Dev1/PFI0", "445 Shutter-MaxVolts": "5.0000", "COM9-Handshaking": "Off", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "XLIGHT Spin motor-Label": "On (spinning)", "PRIME-PP 5 ENABLED": "No", "UUID": "35223bc4-62b2-4881-8ad7-93a6ea046c01", "PRIME-TriggerMode": "Internal Trigger", "COM1-Handshaking": "Off", "PRIME-Timing-PostTriggerDelayNs": "49344000.0000", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "Focus-Position": "17741.5000", "528 Shutter-Block voltage": "false", "Channel": "Il-G", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "555 Shutter-Demo": "No", "405 Shutter-Demo": "No", "528 AO-Name": "AnalogIO", "520-State": "1", "Core-Galvo": "", "405 Shutter-TriggerSampleRate": "100000", "Focus-FocusStepSize_(nm)": "100", "PRIME-PixelType": "16bit", "COM4-DataBits": "8", "470 AO-IOChannel": "Dev1/ao2", "520 AO-MinVolts": "0.0000", "COM9-Parity": "None", "PRIME-CircularBufferEnabled": "ON", "PRIME-PP 6 Reset": "No", "640 AO-Description": "NI DAC", "Source": "PRIME", "445 AO-MinVolts": "0.0000", "PIEZO-Name": "Stage", "445 AO-Description": "NI DAC", "PRIME-CircularBufferFrameCount": "46", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "520 AO-Volts": "4.5000", "528 Shutter-TriggerSampleRate": "100000", "XPositionUm": 19731.9, "OpticalPath-State": "4", "PRIME-PP 3 THRESHOLD": "80", "PRIME-PVCAM Version": "3.7.1", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Gain": "1-HDR", "640 Shutter-TriggerSequenceLength": "1000", "528 AO-SupportsTriggering": "0", "445 AO-TriggerInputLine": "/Dev1/PFI0", "OpticalPath-Name": "OpticalPath", "PRIME-PP 4 ENABLED": "Yes", "PRIME-PVCAM Adapter Version": "1.3.29", "COM3-Name": "COM3", "405 AO-MaxVolts": "5.0000", "520 Shutter-Block voltage": "false", "PRIME-Timing-ClearingTimeNs": "49344000.0000", "Core-Focus": "PIEZO", "528 AO-Block voltage": "false", "TE2000-Description": "Nikon TE2000 hub", "528 AO-MinVolts": "0.0000", "COM4-Parity": "None", "Summary": { "Slices": 11, "ChContrastMin": [ 0, 0, 0, 0, 0, 0 ], "PixelAspect": 1, "SlicesFirst": true, "Interval_ms": 0, "Time": "2019-12-18 21:36:42 -0500", "Prefix": "seq_4", "InitialPositionList": [ { "Label": "Pos0", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.21], "Focus": [17741.5], "XYStage": [ 44542.200000000004, 31636.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos1", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.22], "Focus": [17741.4], "XYStage": [ 43801.100000000006, 31637.9 ] }, "GridColumnIndex": 0 }, { "Label": "Pos2", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.5], "XYStage": [ 38561.3, 32040.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos3", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.185], "Focus": [17741.3], "XYStage": [ 37698.4, 32041.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos4", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.16], "Focus": [17741.5], "XYStage": [ 32375.4, 31758.9 ] }, "GridColumnIndex": 0 }, { "Label": "Pos5", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.17000000000002], "Focus": [17741.3], "XYStage": [ 31770.7, 31758.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos6", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.4], "XYStage": [ 26536.800000000003, 31068.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos7", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.205], "Focus": [17741.4], "XYStage": [ 25420.2, 31068.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos8", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.85], "Focus": [17741.3], "XYStage": [ 21059, 31508 ] }, "GridColumnIndex": 0 }, { "Label": "Pos9", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.82], "Focus": [17741.3], "XYStage": [ 19731.9, 31506.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos10", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.79], "Focus": [17741.4], "XYStage": [ 14528.5, 32007.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos11", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.83], "Focus": [17741.2], "XYStage": [ 13830.300000000001, 32009.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos12", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.79], "Focus": [17741.3], "XYStage": [ 8775.300000000001, 32017.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos13", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.815], "Focus": [17741.3], "XYStage": [ 8109.200000000001, 32008.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos14", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.805], "Focus": [17741.3], "XYStage": [ 3483.3, 31908.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos15", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.82], "Focus": [17741.2], "XYStage": [ 2686.8, 31903.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos16", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [211.48000000000002], "Focus": [17741.1], "XYStage": [ -1978.9, 31973.600000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos17", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [211.51500000000001], "Focus": [17741.1], "XYStage": [ -2957.8, 31982.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos18", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.195], "Focus": [17741.5], "XYStage": [ 47747.4, -576.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos19", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.23000000000002], "Focus": [17741.6], "XYStage": [ 47053.9, -576.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos20", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.205], "Focus": [17741.4], "XYStage": [ 41871.200000000004, -479.8 ] }, "GridColumnIndex": 0 }, { "Label": "Pos21", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.4], "XYStage": [ 40882.600000000006, -392 ] }, "GridColumnIndex": 0 }, { "Label": "Pos22", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.24], "Focus": [17741.4], "XYStage": [ 35905.6, -135.3 ] }, "GridColumnIndex": 0 }, { "Label": "Pos23", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.195], "Focus": [17741.5], "XYStage": [ 34990.9, -132.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos24", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.46], "Focus": [17741.4], "XYStage": [ 29678.100000000002, -958.1 ] }, "GridColumnIndex": 0 }, { "Label": "Pos25", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.44], "Focus": [17741.5], "XYStage": [ 28983.600000000002, -939.4000000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos26", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.475], "Focus": [17741.5], "XYStage": [ 23019.7, -122 ] }, "GridColumnIndex": 0 }, { "Label": "Pos27", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.44], "Focus": [17741.7], "XYStage": [ 22428, -116.80000000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos28", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.46], "Focus": [17741.7], "XYStage": [ 16364.900000000001, -181.10000000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos29", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.48000000000002], "Focus": [17741.5], "XYStage": [ 15712.1, -180 ] }, "GridColumnIndex": 0 }, { "Label": "Pos30", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.45000000000002], "Focus": [17741.7], "XYStage": [ 10346.1, 77.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos31", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.49], "Focus": [17741.4], "XYStage": [ 9755.5, 85.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos32", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.475], "Focus": [17741.5], "XYStage": [ 4775.5, 293.90000000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos33", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.445], "Focus": [17741.4], "XYStage": [ 3954.9, 293.90000000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos34", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.455], "Focus": [17741.4], "XYStage": [ -1324.9, 545.6 ] }, "GridColumnIndex": 0 }, { "Label": "Pos35", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.445], "Focus": [17741.3], "XYStage": [ -2097, 576.6 ] }, "GridColumnIndex": 0 } ], "z-step_um": 10, "GridRow": 0, "BitDepth": 16, "Depth": 2, "Channels": 6, "Source": "Micro-Manager", "ChColors": [ -16711681, -256, -65281, -1, -1, -16776961 ], "MetadataVersion": 10, "KeepShutterOpenChannels": true, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536, 65536 ], "CustomIntervals_ms": [], "Height": 1200, "MicroManagerVersion": "1.4.23 20180219", "GridColumn": 0, "UUID": "14a13bf7-c944-4672-b873-fcfbbb7ea13d", "PixelType": "GRAY16", "Width": 1200, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\Yan\\20191218-YC113-S7S8-miseq", "Frames": 1, "Comment": "", "UserName": "Nikon", "PixelSize_um": 1.1, "ROI": [ 0, 0, 1200, 1200 ], "Date": "2019-12-18", "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC", "P-DAPI-high" ], "KeepShutterOpenSlices": true, "IJType": 1, "ComputerName": "ZadorConfocal2", "Positions": 36, "PositionIndex": 9, "CameraTimeout": 20000, "TimeFirst": false }, "640 AO-Name": "AnalogIO", "Vincent-D3-Command": "Close" }, "FrameKey-0-0-10": { "COM4-StopBits": "1", "528 AO-MaxVolts": "5.0000", "XLIGHT Excitation Wheel-State": "1", "470 Shutter-Name": "AnalogIO", "Core-Shutter": "520", "555 AO-Demo": "No", "XLIGHT Dichroic-State": "2", "XYStage-StepSize-Y": "0.1000", "ZPositionUm": 298.82, "XYStage-StepSize-X": "0.1000", "PRIME-FirmwareVersion": "20.28", "PRIME-ClearCycles": "2", "520-Name": "DA Shutter", "405 Shutter-Name": "AnalogIO", "PIEZO-StepSize": "0.0050", "PRIME-ClearMode": "Pre-Exposure", "470 AO-MinVolts": "0.0000", "405 AO-SupportsTriggering": "1", "CameraChannelIndex": 0, "XYStage-StartSpeed": "500.0000", "COM9-StopBits": "2", "405 AO-Description": "NI DAC", "COM4-DelayBetweenCharsMs": "0.0000", "445 AO-Block voltage": "false", "520 AO-TriggerInputLine": "/Dev1/PFI0", "555 AO-Description": "NI DAC", "520 AO-TriggerSampleRate": "100000", "COM4-Verbose": "1", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM4-Name": "COM4", "405 Shutter-Volts": "0.0000", "Vincent-D3-Port": "COM1", "PRIME-PP 4 MIN ADU AFFECTED": "200", "470 Shutter-MinVolts": "0.0000", "PRIME-PP 3 ENABLED": "Yes", "470 AO-Description": "NI DAC", "PRIME-Description": "PVCAM API device adapter", "520 Shutter-MaxVolts": "5.0000", "520 Shutter-Volts": "5.0000", "528 AO-Sequenceable": "1", "PRIME-Binning": "1x1", "555 Shutter-MaxVolts": "5.0000", "528 Shutter-TriggerSequenceLength": "1000", "405 Shutter-Block voltage": "false", "XLIGHT Spin motor-State": "1", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "Vincent-D3-Time to open (ms)": "35.0000", "Vincent-D3-Time to close (ms)": "35.0000", "445 AO-TriggerSampleRate": "100000", "640 Shutter-IOChannel": "Dev1/ao14", "555 AO-Sequenceable": "1", "520 AO-Name": "AnalogIO", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "470 AO-Block voltage": "false", "COM1-Verbose": "1", "COM9-AnswerTimeout": "10000.0000", "528 Shutter-Name": "AnalogIO", "PRIME-Trigger Timeout (secs)": "10", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "NextFrame": 0, "470 AO-TriggerSequenceLength": "1000", "PRIME-TransposeXY": "0", "445 AO-TriggerSequenceLength": "1000", "COM1-Name": "COM1", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "COM9-DataBits": "8", "COM1-AnswerTimeout": "500.0000", "Camera": "", "TE2000-Version": "V2.50-V2.50-V2.10", "528 Shutter-Volts": "0.0000", "XLIGHT Hub-Name": "XLIGHT Hub", "PIEZO-Description": "Ludl stage driver adapter", "PositionName": "Pos9", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSampleRate": "100000", "PRIME-FanSpeedSetpoint": "High", "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "405 AO-TriggerSampleRate": "100000", "470 Shutter-Block voltage": "false", "470-State": "0", "COM3-Parity": "None", "405 AO-Volts": "0.0000", "PRIME-TransposeMirrorY": "0", "PRIME-ChipName": "GS144BSI", "470 AO-Volts": "0.0000", "PRIME-TransposeMirrorX": "0", "445 Shutter-SupportsTriggering": "0", "470 Shutter-SupportsTriggering": "0", "XLIGHT Hub-Description": "XLIGHT hub", "PRIME-PreampOffLimit": "10000", "470 AO-Sequenceable": "1", "528 Shutter-MaxVolts": "5.0000", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "Core-XYStage": "XYStage", "470 AO-TriggerSampleRate": "100000", "PRIME-Timing-PreTriggerDelayNs": "24672000.0000", "528-DA Device": "528 Shutter", "Focus-Description": "Nikon TE2000 focus adapter", "FilterBlock-State": "5", "520 AO-Demo": "No", "COM3-DelayBetweenCharsMs": "0.0000", "470 Shutter-Volts": "0.0000", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "PRIME-PP 2 THRESHOLD": "125", "PRIME-PP 4 THRESHOLD": "75", "XYStage-Acceleration": "4.0000", "470 Shutter-Demo": "No", "PRIME-Name": "PMPCIECam00", "640-DA Device": "640 Shutter", "405 AO-Block voltage": "false", "ElapsedTime-ms": 380131, "PRIME-Offset": "100", "445 AO-IOChannel": "Dev1/ao1", "640 AO-MaxVolts": "5.0000", "PositionIndex": 9, "PRIME-PP 1 ENABLED": "Yes", "COM1-BaudRate": "9600", "640-State": "0", "COM3-Verbose": "1", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "LudlController-TransmissionDelay": "0", "PRIME-FullWellCapacity": "62000", "PRIME-SMARTStreamingEnabled": "No", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "640 Shutter-Description": "NI DAC", "528-Description": "DA device that is used as a shutter", "COM4-Description": "Serial port driver (boost:asio)", "405 Shutter-Description": "NI DAC", "TE2000-Name": "TE2000", "528 Shutter-Description": "NI DAC", "COM9-Description": "Serial port driver (boost:asio)", "LudlController-Name": "LudlController", "445 Shutter-TriggerSequenceLength": "1000", "PRIME-SerialNumber": "A18B203008", "445 AO-Volts": "0.0000", "PRIME-X-dimension": "1200", "PRIME-ShutterCloseDelay": "0", "XLIGHT Disk slider-State": "2", "Position": "Default", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 AO-IOChannel": "Dev1/ao5", "520 AO-IOChannel": "Dev1/ao4", "528 AO-TriggerSequenceLength": "1000", "405 Shutter-MaxVolts": "5.0000", "470-DA Device": "470 Shutter", "PRIME-FTCapable": "No", "Core-ChannelGroup": "Channels", "555 AO-SupportsTriggering": "0", "PRIME-Y-dimension": "1200", "Frame": 0, "555 Shutter-Sequenceable": "1", "PRIME-Timing-ExposureTimeNs": "499998000.0000", "Core-ImageProcessor": "", "528-State": "0", "520 AO-Block voltage": "false", "XLIGHT Excitation Wheel-Label": "CFP/YFP", "640 Shutter-Demo": "No", "COM3-StopBits": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "520 Shutter-IOChannel": "Dev1/ao11", "Width": 1200, "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-SupportsTriggering": "0", "640 AO-TriggerSampleRate": "100000", "520-Description": "DA device that is used as a shutter", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "405 Shutter-Sequenceable": "1", "405 Shutter-SupportsTriggering": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-TriggerSampleRate": "100000", "640 Shutter-MaxVolts": "5.0000", "555 AO-MaxVolts": "5.0000", "520 AO-TriggerSequenceLength": "1000", "COM3-BaudRate": "9600", "COM9-BaudRate": "9600", "Vincent-D3-Channel #": "Ch. #1", "PRIME-PMode": "Normal", "OpticalPath-Label": "Left(100)", "BitDepth": 16, "555 AO-TriggerSequenceLength": "1000", "445 Shutter-Description": "NI DAC", "405 AO-Sequenceable": "1", "PRIME-AcquisitionMethod": "Callbacks", "555 AO-Name": "AnalogIO", "PRIME-ShutterMode": "Pre-Exposure", "COM3-Handshaking": "Off", "445 AO-SupportsTriggering": "0", "ROI": "0-0-1200-1200", "COM9-Verbose": "1", "PRIME-Color": "OFF", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "PRIME-ShutterOpenDelay": "0", "COM1-Parity": "None", "Slice": 10, "SlicePosition": 50, "COM9-DelayBetweenCharsMs": "0.0000", "555-Name": "DA Shutter", "PIEZO-LudlSingleAxisName": "B", "XLIGHT Emission Wheel-Label": "565/24(G)", "640 Shutter-Name": "AnalogIO", "640 AO-Sequenceable": "1", "520 Shutter-Demo": "No", "528 Shutter-Demo": "No", "470-Name": "DA Shutter", "520-DA Device": "520 Shutter", "XYStage-Description": "Ludl XY stage driver adapter", "445 Shutter-MinVolts": "0.0000", "528 AO-TriggerSampleRate": "100000", "405 AO-MinVolts": "0.0000", "405 Shutter-TriggerSequenceLength": "1000", "Height": 1200, "555 AO-Block voltage": "false", "640 Shutter-Volts": "0.0000", "PixelType": "GRAY16", "445 Shutter-Block voltage": "false", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "520 Shutter-TriggerSequenceLength": "1000", "COM9-Name": "COM9", "Core-SLM": "", "555 AO-Volts": "0.0000", "555 Shutter-Block voltage": "false", "XYStage-Speed": "1800.0000", "520 AO-MaxVolts": "5.0000", "528 Shutter-SupportsTriggering": "0", "COM1-Description": "Serial port driver (boost:asio)", "PRIME-MetadataEnabled": "No", "520 Shutter-MinVolts": "0.0000", "470 Shutter-Sequenceable": "1", "PRIME-PreampDelay": "0", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-MinVolts": "0.0000", "ChannelIndex": 0, "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Core-AutoShutter": "1", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "Exposure-ms": 320, "PRIME-PP 2 ENABLED": "Yes", "640 Shutter-TriggerSampleRate": "100000", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "470 Shutter-IOChannel": "Dev1/ao10", "405 AO-Demo": "No", "PixelSizeUm": 1.1, "470 AO-Demo": "No", "470 Shutter-MaxVolts": "5.0000", "Vincent-D3-Name": "Vincent-D3", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FileName": "img_000000000_Il-G_010.tif", "LudlController-Reset": "Operate", "PRIME-Exposure": "320.00", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "445-Name": "DA Shutter", "555 Shutter-Name": "AnalogIO", "520 Shutter-TriggerSampleRate": "100000", "445 Shutter-IOChannel": "Dev1/ao9", "PRIME-TransposeCorrection": "0", "XYStage-StepSize": "0.1000", "445-Description": "DA device that is used as a shutter", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 AO-IOChannel": "Dev1/ao7", "520 Shutter-Name": "AnalogIO", "COM3-DataBits": "8", "640 AO-Volts": "0.0000", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "555 Shutter-IOChannel": "Dev1/ao13", "WaitInterval": null, "520 Shutter-Sequenceable": "1", "YPositionUm": 31506.2, "445-State": "0", "445-DA Device": "445 Shutter", "PRIME-CircularBufferAutoSize": "ON", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "555 AO-TriggerSampleRate": "100000", "445 Shutter-Volts": "0.0000", "XLIGHT Hub-Port": "COM3", "Core-AutoFocus": "", "COM4-Handshaking": "Off", "528 Shutter-MinVolts": "0.0000", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "445 Shutter-Name": "AnalogIO", "528 Shutter-Sequenceable": "1", "XYStage-TransposeMirrorX": "0", "XYStage-TransposeMirrorY": "0", "PRIME-ReadoutRate": "100MHz 16bit", "XYStage-Name": "Stage", "640 AO-SupportsTriggering": "0", "640-Name": "DA Shutter", "640 Shutter-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "640 AO-Block voltage": "false", "445 Shutter-Sequenceable": "1", "470 AO-MaxVolts": "5.0000", "COM4-BaudRate": "9600", "470-Description": "DA device that is used as a shutter", "520 Shutter-Description": "NI DAC", "PRIME-CCDTemperature": "-14.8500", "Time": "2019-12-18 21:36:48 -0500", "640 AO-TriggerSequenceLength": "1000", "470 AO-Name": "AnalogIO", "XLIGHT Disk slider-Label": "Disk Pos 40um", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "Binning": "1x1", "TE2000-ModelType": "1", "COM3-AnswerTimeout": "500.0000", "555 Shutter-SupportsTriggering": "0", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "TE2000-Port": "COM4", "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Description": "NI DAC", "COM4-AnswerTimeout": "500.0000", "COM1-DelayBetweenCharsMs": "0.0000", "FilterBlock-Label": "Confocal", "445 AO-Name": "AnalogIO", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-Timing-ReadoutTimeNs": "24672000.0000", "528 AO-Demo": "No", "520 AO-Sequenceable": "1", "555-Description": "DA device that is used as a shutter", "405-DA Device": "405 Shutter", "405 AO-TriggerSequenceLength": "1000", "640 AO-MinVolts": "0.0000", "640 Shutter-Block voltage": "false", "FrameIndex": 0, "PRIME-Port": "CMOS", "555 AO-MinVolts": "0.0000", "COM3-Description": "Serial port driver (boost:asio)", "528 AO-Description": "NI DAC", "555 AO-IOChannel": "Dev1/ao6", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "555 AO-TriggerInputLine": "/Dev1/PFI0", "PRIME-ActualInterval-ms": "390.5200", "LudlController-Port": "COM9", "445 Shutter-Demo": "No", "PRIME-Trigger-Expose Out-Mux": "1", "SliceIndex": 10, "PRIME-CCDTemperatureSetPoint": "-15.0000", "405 Shutter-MinVolts": "0.0000", "PRIME-ExposeOutMode": "First Row", "AxisPositions": null, "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Volts": "0.0000", "528-Name": "DA Shutter", "555-State": "0", "XLIGHT Dichroic-Label": "CFP/YFP", "445 AO-Sequenceable": "1", "405-Name": "DA Shutter", "405 AO-IOChannel": "Dev1/ao0", "Core-Camera": "PRIME", "Focus-Name": "Focus", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "405 Shutter-IOChannel": "Dev1/ao8", "640 AO-Demo": "No", "640 Shutter-SupportsTriggering": "0", "445 AO-Demo": "No", "555-DA Device": "555 Shutter", "470 Shutter-Description": "NI DAC", "528 AO-Volts": "0.0000", "COM1-DataBits": "8", "Vincent-D3-Address": "x", "520 AO-SupportsTriggering": "0", "405-Description": "DA device that is used as a shutter", "640 Shutter-MinVolts": "0.0000", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "405 AO-Name": "AnalogIO", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "640 AO-TriggerInputLine": "/Dev1/PFI0", "405 AO-TriggerInputLine": "/Dev1/PFI0", "640-Description": "DA device that is used as a shutter", "445 Shutter-TriggerSampleRate": "100000", "470 Shutter-TriggerSequenceLength": "1000", "COM1-StopBits": "1", "520 Shutter-SupportsTriggering": "0", "405-State": "0", "Core-Initialize": "1", "XLIGHT Emission Wheel-State": "3", "520 AO-Description": "NI DAC", "528 AO-TriggerInputLine": "/Dev1/PFI0", "445 Shutter-MaxVolts": "5.0000", "COM9-Handshaking": "Off", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "XLIGHT Spin motor-Label": "On (spinning)", "PRIME-PP 5 ENABLED": "No", "UUID": "0a3e7d27-dfad-46b7-9d77-48eee2a48c05", "PRIME-TriggerMode": "Internal Trigger", "COM1-Handshaking": "Off", "PRIME-Timing-PostTriggerDelayNs": "49344000.0000", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "Focus-Position": "17741.5000", "528 Shutter-Block voltage": "false", "Channel": "Il-G", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "555 Shutter-Demo": "No", "405 Shutter-Demo": "No", "528 AO-Name": "AnalogIO", "520-State": "0", "Core-Galvo": "", "405 Shutter-TriggerSampleRate": "100000", "Focus-FocusStepSize_(nm)": "100", "PRIME-PixelType": "16bit", "COM4-DataBits": "8", "470 AO-IOChannel": "Dev1/ao2", "520 AO-MinVolts": "0.0000", "COM9-Parity": "None", "PRIME-CircularBufferEnabled": "ON", "PRIME-PP 6 Reset": "No", "640 AO-Description": "NI DAC", "Source": "PRIME", "445 AO-MinVolts": "0.0000", "PIEZO-Name": "Stage", "445 AO-Description": "NI DAC", "PRIME-CircularBufferFrameCount": "46", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "520 AO-Volts": "4.5000", "528 Shutter-TriggerSampleRate": "100000", "XPositionUm": 19731.9, "OpticalPath-State": "4", "PRIME-PP 3 THRESHOLD": "80", "PRIME-PVCAM Version": "3.7.1", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Gain": "1-HDR", "640 Shutter-TriggerSequenceLength": "1000", "528 AO-SupportsTriggering": "0", "445 AO-TriggerInputLine": "/Dev1/PFI0", "OpticalPath-Name": "OpticalPath", "PRIME-PP 4 ENABLED": "Yes", "PRIME-PVCAM Adapter Version": "1.3.29", "COM3-Name": "COM3", "405 AO-MaxVolts": "5.0000", "520 Shutter-Block voltage": "false", "PRIME-Timing-ClearingTimeNs": "49344000.0000", "Core-Focus": "PIEZO", "528 AO-Block voltage": "false", "TE2000-Description": "Nikon TE2000 hub", "528 AO-MinVolts": "0.0000", "COM4-Parity": "None", "Summary": { "Slices": 11, "ChContrastMin": [ 0, 0, 0, 0, 0, 0 ], "PixelAspect": 1, "SlicesFirst": true, "Interval_ms": 0, "Time": "2019-12-18 21:36:42 -0500", "Prefix": "seq_4", "InitialPositionList": [ { "Label": "Pos0", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.21], "Focus": [17741.5], "XYStage": [ 44542.200000000004, 31636.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos1", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.22], "Focus": [17741.4], "XYStage": [ 43801.100000000006, 31637.9 ] }, "GridColumnIndex": 0 }, { "Label": "Pos2", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.5], "XYStage": [ 38561.3, 32040.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos3", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.185], "Focus": [17741.3], "XYStage": [ 37698.4, 32041.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos4", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.16], "Focus": [17741.5], "XYStage": [ 32375.4, 31758.9 ] }, "GridColumnIndex": 0 }, { "Label": "Pos5", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.17000000000002], "Focus": [17741.3], "XYStage": [ 31770.7, 31758.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos6", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.4], "XYStage": [ 26536.800000000003, 31068.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos7", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.205], "Focus": [17741.4], "XYStage": [ 25420.2, 31068.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos8", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.85], "Focus": [17741.3], "XYStage": [ 21059, 31508 ] }, "GridColumnIndex": 0 }, { "Label": "Pos9", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.82], "Focus": [17741.3], "XYStage": [ 19731.9, 31506.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos10", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.79], "Focus": [17741.4], "XYStage": [ 14528.5, 32007.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos11", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.83], "Focus": [17741.2], "XYStage": [ 13830.300000000001, 32009.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos12", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.79], "Focus": [17741.3], "XYStage": [ 8775.300000000001, 32017.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos13", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.815], "Focus": [17741.3], "XYStage": [ 8109.200000000001, 32008.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos14", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.805], "Focus": [17741.3], "XYStage": [ 3483.3, 31908.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos15", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.82], "Focus": [17741.2], "XYStage": [ 2686.8, 31903.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos16", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [211.48000000000002], "Focus": [17741.1], "XYStage": [ -1978.9, 31973.600000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos17", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [211.51500000000001], "Focus": [17741.1], "XYStage": [ -2957.8, 31982.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos18", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.195], "Focus": [17741.5], "XYStage": [ 47747.4, -576.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos19", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.23000000000002], "Focus": [17741.6], "XYStage": [ 47053.9, -576.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos20", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.205], "Focus": [17741.4], "XYStage": [ 41871.200000000004, -479.8 ] }, "GridColumnIndex": 0 }, { "Label": "Pos21", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.4], "XYStage": [ 40882.600000000006, -392 ] }, "GridColumnIndex": 0 }, { "Label": "Pos22", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.24], "Focus": [17741.4], "XYStage": [ 35905.6, -135.3 ] }, "GridColumnIndex": 0 }, { "Label": "Pos23", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.195], "Focus": [17741.5], "XYStage": [ 34990.9, -132.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos24", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.46], "Focus": [17741.4], "XYStage": [ 29678.100000000002, -958.1 ] }, "GridColumnIndex": 0 }, { "Label": "Pos25", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.44], "Focus": [17741.5], "XYStage": [ 28983.600000000002, -939.4000000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos26", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.475], "Focus": [17741.5], "XYStage": [ 23019.7, -122 ] }, "GridColumnIndex": 0 }, { "Label": "Pos27", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.44], "Focus": [17741.7], "XYStage": [ 22428, -116.80000000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos28", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.46], "Focus": [17741.7], "XYStage": [ 16364.900000000001, -181.10000000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos29", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.48000000000002], "Focus": [17741.5], "XYStage": [ 15712.1, -180 ] }, "GridColumnIndex": 0 }, { "Label": "Pos30", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.45000000000002], "Focus": [17741.7], "XYStage": [ 10346.1, 77.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos31", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.49], "Focus": [17741.4], "XYStage": [ 9755.5, 85.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos32", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.475], "Focus": [17741.5], "XYStage": [ 4775.5, 293.90000000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos33", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.445], "Focus": [17741.4], "XYStage": [ 3954.9, 293.90000000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos34", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.455], "Focus": [17741.4], "XYStage": [ -1324.9, 545.6 ] }, "GridColumnIndex": 0 }, { "Label": "Pos35", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.445], "Focus": [17741.3], "XYStage": [ -2097, 576.6 ] }, "GridColumnIndex": 0 } ], "z-step_um": 10, "GridRow": 0, "BitDepth": 16, "Depth": 2, "Channels": 6, "Source": "Micro-Manager", "ChColors": [ -16711681, -256, -65281, -1, -1, -16776961 ], "MetadataVersion": 10, "KeepShutterOpenChannels": true, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536, 65536 ], "CustomIntervals_ms": [], "Height": 1200, "MicroManagerVersion": "1.4.23 20180219", "GridColumn": 0, "UUID": "14a13bf7-c944-4672-b873-fcfbbb7ea13d", "PixelType": "GRAY16", "Width": 1200, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\Yan\\20191218-YC113-S7S8-miseq", "Frames": 1, "Comment": "", "UserName": "Nikon", "PixelSize_um": 1.1, "ROI": [ 0, 0, 1200, 1200 ], "Date": "2019-12-18", "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC", "P-DAPI-high" ], "KeepShutterOpenSlices": true, "IJType": 1, "ComputerName": "ZadorConfocal2", "Positions": 36, "PositionIndex": 9, "CameraTimeout": 20000, "TimeFirst": false }, "640 AO-Name": "AnalogIO", "Vincent-D3-Command": "Close" }, "FrameKey-0-1-0": { "COM4-StopBits": "1", "528 AO-MaxVolts": "5.0000", "XLIGHT Excitation Wheel-State": "1", "470 Shutter-Name": "AnalogIO", "Core-Shutter": "555", "555 AO-Demo": "No", "XLIGHT Dichroic-State": "2", "XYStage-StepSize-Y": "0.1000", "ZPositionUm": 198.82, "XYStage-StepSize-X": "0.1000", "PRIME-FirmwareVersion": "20.28", "PRIME-ClearCycles": "2", "520-Name": "DA Shutter", "405 Shutter-Name": "AnalogIO", "PIEZO-StepSize": "0.0050", "PRIME-ClearMode": "Pre-Exposure", "470 AO-MinVolts": "0.0000", "405 AO-SupportsTriggering": "1", "CameraChannelIndex": 0, "XYStage-StartSpeed": "500.0000", "COM9-StopBits": "2", "405 AO-Description": "NI DAC", "COM4-DelayBetweenCharsMs": "0.0000", "445 AO-Block voltage": "false", "520 AO-TriggerInputLine": "/Dev1/PFI0", "555 AO-Description": "NI DAC", "520 AO-TriggerSampleRate": "100000", "COM4-Verbose": "1", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM4-Name": "COM4", "405 Shutter-Volts": "0.0000", "Vincent-D3-Port": "COM1", "PRIME-PP 4 MIN ADU AFFECTED": "200", "470 Shutter-MinVolts": "0.0000", "PRIME-PP 3 ENABLED": "Yes", "470 AO-Description": "NI DAC", "PRIME-Description": "PVCAM API device adapter", "520 Shutter-MaxVolts": "5.0000", "520 Shutter-Volts": "0.0000", "528 AO-Sequenceable": "1", "PRIME-Binning": "1x1", "555 Shutter-MaxVolts": "5.0000", "528 Shutter-TriggerSequenceLength": "1000", "405 Shutter-Block voltage": "false", "XLIGHT Spin motor-State": "1", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "Vincent-D3-Time to open (ms)": "35.0000", "Vincent-D3-Time to close (ms)": "35.0000", "445 AO-TriggerSampleRate": "100000", "640 Shutter-IOChannel": "Dev1/ao14", "555 AO-Sequenceable": "1", "520 AO-Name": "AnalogIO", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "470 AO-Block voltage": "false", "COM1-Verbose": "1", "COM9-AnswerTimeout": "10000.0000", "528 Shutter-Name": "AnalogIO", "PRIME-Trigger Timeout (secs)": "10", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "NextFrame": 0, "470 AO-TriggerSequenceLength": "1000", "PRIME-TransposeXY": "0", "445 AO-TriggerSequenceLength": "1000", "COM1-Name": "COM1", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "COM9-DataBits": "8", "COM1-AnswerTimeout": "500.0000", "Camera": "", "TE2000-Version": "V2.50-V2.50-V2.10", "528 Shutter-Volts": "0.0000", "XLIGHT Hub-Name": "XLIGHT Hub", "PIEZO-Description": "Ludl stage driver adapter", "PositionName": "Pos9", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSampleRate": "100000", "PRIME-FanSpeedSetpoint": "High", "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "405 AO-TriggerSampleRate": "100000", "470 Shutter-Block voltage": "false", "470-State": "0", "COM3-Parity": "None", "405 AO-Volts": "0.0000", "PRIME-TransposeMirrorY": "0", "PRIME-ChipName": "GS144BSI", "470 AO-Volts": "0.0000", "PRIME-TransposeMirrorX": "0", "445 Shutter-SupportsTriggering": "0", "470 Shutter-SupportsTriggering": "0", "XLIGHT Hub-Description": "XLIGHT hub", "PRIME-PreampOffLimit": "10000", "470 AO-Sequenceable": "1", "528 Shutter-MaxVolts": "5.0000", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "Core-XYStage": "XYStage", "470 AO-TriggerSampleRate": "100000", "PRIME-Timing-PreTriggerDelayNs": "24672000.0000", "528-DA Device": "528 Shutter", "Focus-Description": "Nikon TE2000 focus adapter", "FilterBlock-State": "5", "520 AO-Demo": "No", "COM3-DelayBetweenCharsMs": "0.0000", "470 Shutter-Volts": "0.0000", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "PRIME-PP 2 THRESHOLD": "125", "PRIME-PP 4 THRESHOLD": "75", "XYStage-Acceleration": "4.0000", "470 Shutter-Demo": "No", "PRIME-Name": "PMPCIECam00", "640-DA Device": "640 Shutter", "405 AO-Block voltage": "false", "ElapsedTime-ms": 381409, "PRIME-Offset": "100", "445 AO-IOChannel": "Dev1/ao1", "640 AO-MaxVolts": "5.0000", "PositionIndex": 9, "PRIME-PP 1 ENABLED": "Yes", "COM1-BaudRate": "9600", "640-State": "0", "COM3-Verbose": "1", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "LudlController-TransmissionDelay": "0", "PRIME-FullWellCapacity": "62000", "PRIME-SMARTStreamingEnabled": "No", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "640 Shutter-Description": "NI DAC", "528-Description": "DA device that is used as a shutter", "COM4-Description": "Serial port driver (boost:asio)", "405 Shutter-Description": "NI DAC", "TE2000-Name": "TE2000", "528 Shutter-Description": "NI DAC", "COM9-Description": "Serial port driver (boost:asio)", "LudlController-Name": "LudlController", "445 Shutter-TriggerSequenceLength": "1000", "PRIME-SerialNumber": "A18B203008", "445 AO-Volts": "0.0000", "PRIME-X-dimension": "1200", "PRIME-ShutterCloseDelay": "0", "XLIGHT Disk slider-State": "2", "Position": "Default", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 AO-IOChannel": "Dev1/ao5", "520 AO-IOChannel": "Dev1/ao4", "528 AO-TriggerSequenceLength": "1000", "405 Shutter-MaxVolts": "5.0000", "470-DA Device": "470 Shutter", "PRIME-FTCapable": "No", "Core-ChannelGroup": "Channels", "555 AO-SupportsTriggering": "0", "PRIME-Y-dimension": "1200", "Frame": 0, "555 Shutter-Sequenceable": "1", "PRIME-Timing-ExposureTimeNs": "499998000.0000", "Core-ImageProcessor": "", "528-State": "0", "520 AO-Block voltage": "false", "XLIGHT Excitation Wheel-Label": "QUAD", "640 Shutter-Demo": "No", "COM3-StopBits": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "520 Shutter-IOChannel": "Dev1/ao11", "Width": 1200, "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-SupportsTriggering": "0", "640 AO-TriggerSampleRate": "100000", "520-Description": "DA device that is used as a shutter", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "405 Shutter-Sequenceable": "1", "405 Shutter-SupportsTriggering": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-TriggerSampleRate": "100000", "640 Shutter-MaxVolts": "5.0000", "555 AO-MaxVolts": "5.0000", "520 AO-TriggerSequenceLength": "1000", "COM3-BaudRate": "9600", "COM9-BaudRate": "9600", "Vincent-D3-Channel #": "Ch. #1", "PRIME-PMode": "Normal", "OpticalPath-Label": "Left(100)", "BitDepth": 16, "555 AO-TriggerSequenceLength": "1000", "445 Shutter-Description": "NI DAC", "405 AO-Sequenceable": "1", "PRIME-AcquisitionMethod": "Callbacks", "555 AO-Name": "AnalogIO", "PRIME-ShutterMode": "Pre-Exposure", "COM3-Handshaking": "Off", "445 AO-SupportsTriggering": "0", "ROI": "0-0-1200-1200", "COM9-Verbose": "1", "PRIME-Color": "OFF", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "PRIME-ShutterOpenDelay": "0", "COM1-Parity": "None", "Slice": 0, "SlicePosition": -50, "COM9-DelayBetweenCharsMs": "0.0000", "555-Name": "DA Shutter", "PIEZO-LudlSingleAxisName": "B", "XLIGHT Emission Wheel-Label": "585/11(T)", "640 Shutter-Name": "AnalogIO", "640 AO-Sequenceable": "1", "520 Shutter-Demo": "No", "528 Shutter-Demo": "No", "470-Name": "DA Shutter", "520-DA Device": "520 Shutter", "XYStage-Description": "Ludl XY stage driver adapter", "445 Shutter-MinVolts": "0.0000", "528 AO-TriggerSampleRate": "100000", "405 AO-MinVolts": "0.0000", "405 Shutter-TriggerSequenceLength": "1000", "Height": 1200, "555 AO-Block voltage": "false", "640 Shutter-Volts": "0.0000", "PixelType": "GRAY16", "445 Shutter-Block voltage": "false", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "520 Shutter-TriggerSequenceLength": "1000", "COM9-Name": "COM9", "Core-SLM": "", "555 AO-Volts": "4.5000", "555 Shutter-Block voltage": "false", "XYStage-Speed": "1800.0000", "520 AO-MaxVolts": "5.0000", "528 Shutter-SupportsTriggering": "0", "COM1-Description": "Serial port driver (boost:asio)", "PRIME-MetadataEnabled": "No", "520 Shutter-MinVolts": "0.0000", "470 Shutter-Sequenceable": "1", "PRIME-PreampDelay": "0", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-MinVolts": "0.0000", "ChannelIndex": 1, "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Core-AutoShutter": "1", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "Exposure-ms": 160, "PRIME-PP 2 ENABLED": "Yes", "640 Shutter-TriggerSampleRate": "100000", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "470 Shutter-IOChannel": "Dev1/ao10", "405 AO-Demo": "No", "PixelSizeUm": 1.1, "470 AO-Demo": "No", "470 Shutter-MaxVolts": "5.0000", "Vincent-D3-Name": "Vincent-D3", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FileName": "img_000000000_Il-T_000.tif", "LudlController-Reset": "Operate", "PRIME-Exposure": "160.00", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "445-Name": "DA Shutter", "555 Shutter-Name": "AnalogIO", "520 Shutter-TriggerSampleRate": "100000", "445 Shutter-IOChannel": "Dev1/ao9", "PRIME-TransposeCorrection": "0", "XYStage-StepSize": "0.1000", "445-Description": "DA device that is used as a shutter", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 AO-IOChannel": "Dev1/ao7", "520 Shutter-Name": "AnalogIO", "COM3-DataBits": "8", "640 AO-Volts": "0.0000", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "555 Shutter-IOChannel": "Dev1/ao13", "WaitInterval": null, "520 Shutter-Sequenceable": "1", "YPositionUm": 31506.2, "445-State": "0", "445-DA Device": "445 Shutter", "PRIME-CircularBufferAutoSize": "ON", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "555 AO-TriggerSampleRate": "100000", "445 Shutter-Volts": "0.0000", "XLIGHT Hub-Port": "COM3", "Core-AutoFocus": "", "COM4-Handshaking": "Off", "528 Shutter-MinVolts": "0.0000", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "445 Shutter-Name": "AnalogIO", "528 Shutter-Sequenceable": "1", "XYStage-TransposeMirrorX": "0", "XYStage-TransposeMirrorY": "0", "PRIME-ReadoutRate": "100MHz 16bit", "XYStage-Name": "Stage", "640 AO-SupportsTriggering": "0", "640-Name": "DA Shutter", "640 Shutter-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "640 AO-Block voltage": "false", "445 Shutter-Sequenceable": "1", "470 AO-MaxVolts": "5.0000", "COM4-BaudRate": "9600", "470-Description": "DA device that is used as a shutter", "520 Shutter-Description": "NI DAC", "PRIME-CCDTemperature": "-14.8500", "Time": "2019-12-18 21:36:49 -0500", "640 AO-TriggerSequenceLength": "1000", "470 AO-Name": "AnalogIO", "XLIGHT Disk slider-Label": "Disk Pos 40um", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "Binning": "1x1", "TE2000-ModelType": "1", "COM3-AnswerTimeout": "500.0000", "555 Shutter-SupportsTriggering": "0", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "TE2000-Port": "COM4", "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Description": "NI DAC", "COM4-AnswerTimeout": "500.0000", "COM1-DelayBetweenCharsMs": "0.0000", "FilterBlock-Label": "Confocal", "445 AO-Name": "AnalogIO", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-Timing-ReadoutTimeNs": "24672000.0000", "528 AO-Demo": "No", "520 AO-Sequenceable": "1", "555-Description": "DA device that is used as a shutter", "405-DA Device": "405 Shutter", "405 AO-TriggerSequenceLength": "1000", "640 AO-MinVolts": "0.0000", "640 Shutter-Block voltage": "false", "FrameIndex": 0, "PRIME-Port": "CMOS", "555 AO-MinVolts": "0.0000", "COM3-Description": "Serial port driver (boost:asio)", "528 AO-Description": "NI DAC", "555 AO-IOChannel": "Dev1/ao6", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "555 AO-TriggerInputLine": "/Dev1/PFI0", "PRIME-ActualInterval-ms": "390.5200", "LudlController-Port": "COM9", "445 Shutter-Demo": "No", "PRIME-Trigger-Expose Out-Mux": "1", "SliceIndex": 0, "PRIME-CCDTemperatureSetPoint": "-15.0000", "405 Shutter-MinVolts": "0.0000", "PRIME-ExposeOutMode": "First Row", "AxisPositions": null, "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Volts": "5.0000", "528-Name": "DA Shutter", "555-State": "1", "XLIGHT Dichroic-Label": "QUAD", "445 AO-Sequenceable": "1", "405-Name": "DA Shutter", "405 AO-IOChannel": "Dev1/ao0", "Core-Camera": "PRIME", "Focus-Name": "Focus", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "405 Shutter-IOChannel": "Dev1/ao8", "640 AO-Demo": "No", "640 Shutter-SupportsTriggering": "0", "445 AO-Demo": "No", "555-DA Device": "555 Shutter", "470 Shutter-Description": "NI DAC", "528 AO-Volts": "0.0000", "COM1-DataBits": "8", "Vincent-D3-Address": "x", "520 AO-SupportsTriggering": "0", "405-Description": "DA device that is used as a shutter", "640 Shutter-MinVolts": "0.0000", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "405 AO-Name": "AnalogIO", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "640 AO-TriggerInputLine": "/Dev1/PFI0", "405 AO-TriggerInputLine": "/Dev1/PFI0", "640-Description": "DA device that is used as a shutter", "445 Shutter-TriggerSampleRate": "100000", "470 Shutter-TriggerSequenceLength": "1000", "COM1-StopBits": "1", "520 Shutter-SupportsTriggering": "0", "405-State": "0", "Core-Initialize": "1", "XLIGHT Emission Wheel-State": "3", "520 AO-Description": "NI DAC", "528 AO-TriggerInputLine": "/Dev1/PFI0", "445 Shutter-MaxVolts": "5.0000", "COM9-Handshaking": "Off", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "XLIGHT Spin motor-Label": "On (spinning)", "PRIME-PP 5 ENABLED": "No", "UUID": "c4dc05ea-3ff1-4848-8e9d-8cd626160b2c", "PRIME-TriggerMode": "Internal Trigger", "COM1-Handshaking": "Off", "PRIME-Timing-PostTriggerDelayNs": "49344000.0000", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "Focus-Position": "17741.5000", "528 Shutter-Block voltage": "false", "Channel": "Il-T", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "555 Shutter-Demo": "No", "405 Shutter-Demo": "No", "528 AO-Name": "AnalogIO", "520-State": "0", "Core-Galvo": "", "405 Shutter-TriggerSampleRate": "100000", "Focus-FocusStepSize_(nm)": "100", "PRIME-PixelType": "16bit", "COM4-DataBits": "8", "470 AO-IOChannel": "Dev1/ao2", "520 AO-MinVolts": "0.0000", "COM9-Parity": "None", "PRIME-CircularBufferEnabled": "ON", "PRIME-PP 6 Reset": "No", "640 AO-Description": "NI DAC", "Source": "PRIME", "445 AO-MinVolts": "0.0000", "PIEZO-Name": "Stage", "445 AO-Description": "NI DAC", "PRIME-CircularBufferFrameCount": "46", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "520 AO-Volts": "0.0000", "528 Shutter-TriggerSampleRate": "100000", "XPositionUm": 19731.9, "OpticalPath-State": "4", "PRIME-PP 3 THRESHOLD": "80", "PRIME-PVCAM Version": "3.7.1", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Gain": "1-HDR", "640 Shutter-TriggerSequenceLength": "1000", "528 AO-SupportsTriggering": "0", "445 AO-TriggerInputLine": "/Dev1/PFI0", "OpticalPath-Name": "OpticalPath", "PRIME-PP 4 ENABLED": "Yes", "PRIME-PVCAM Adapter Version": "1.3.29", "COM3-Name": "COM3", "405 AO-MaxVolts": "5.0000", "520 Shutter-Block voltage": "false", "PRIME-Timing-ClearingTimeNs": "49344000.0000", "Core-Focus": "PIEZO", "528 AO-Block voltage": "false", "TE2000-Description": "Nikon TE2000 hub", "528 AO-MinVolts": "0.0000", "COM4-Parity": "None", "Summary": { "Slices": 11, "ChContrastMin": [ 0, 0, 0, 0, 0, 0 ], "PixelAspect": 1, "SlicesFirst": true, "Interval_ms": 0, "Time": "2019-12-18 21:36:42 -0500", "Prefix": "seq_4", "InitialPositionList": [ { "Label": "Pos0", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.21], "Focus": [17741.5], "XYStage": [ 44542.200000000004, 31636.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos1", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.22], "Focus": [17741.4], "XYStage": [ 43801.100000000006, 31637.9 ] }, "GridColumnIndex": 0 }, { "Label": "Pos2", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.5], "XYStage": [ 38561.3, 32040.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos3", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.185], "Focus": [17741.3], "XYStage": [ 37698.4, 32041.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos4", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.16], "Focus": [17741.5], "XYStage": [ 32375.4, 31758.9 ] }, "GridColumnIndex": 0 }, { "Label": "Pos5", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.17000000000002], "Focus": [17741.3], "XYStage": [ 31770.7, 31758.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos6", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.4], "XYStage": [ 26536.800000000003, 31068.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos7", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.205], "Focus": [17741.4], "XYStage": [ 25420.2, 31068.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos8", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.85], "Focus": [17741.3], "XYStage": [ 21059, 31508 ] }, "GridColumnIndex": 0 }, { "Label": "Pos9", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.82], "Focus": [17741.3], "XYStage": [ 19731.9, 31506.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos10", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.79], "Focus": [17741.4], "XYStage": [ 14528.5, 32007.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos11", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.83], "Focus": [17741.2], "XYStage": [ 13830.300000000001, 32009.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos12", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.79], "Focus": [17741.3], "XYStage": [ 8775.300000000001, 32017.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos13", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.815], "Focus": [17741.3], "XYStage": [ 8109.200000000001, 32008.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos14", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.805], "Focus": [17741.3], "XYStage": [ 3483.3, 31908.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos15", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.82], "Focus": [17741.2], "XYStage": [ 2686.8, 31903.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos16", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [211.48000000000002], "Focus": [17741.1], "XYStage": [ -1978.9, 31973.600000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos17", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [211.51500000000001], "Focus": [17741.1], "XYStage": [ -2957.8, 31982.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos18", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.195], "Focus": [17741.5], "XYStage": [ 47747.4, -576.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos19", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.23000000000002], "Focus": [17741.6], "XYStage": [ 47053.9, -576.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos20", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.205], "Focus": [17741.4], "XYStage": [ 41871.200000000004, -479.8 ] }, "GridColumnIndex": 0 }, { "Label": "Pos21", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.4], "XYStage": [ 40882.600000000006, -392 ] }, "GridColumnIndex": 0 }, { "Label": "Pos22", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.24], "Focus": [17741.4], "XYStage": [ 35905.6, -135.3 ] }, "GridColumnIndex": 0 }, { "Label": "Pos23", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.195], "Focus": [17741.5], "XYStage": [ 34990.9, -132.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos24", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.46], "Focus": [17741.4], "XYStage": [ 29678.100000000002, -958.1 ] }, "GridColumnIndex": 0 }, { "Label": "Pos25", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.44], "Focus": [17741.5], "XYStage": [ 28983.600000000002, -939.4000000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos26", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.475], "Focus": [17741.5], "XYStage": [ 23019.7, -122 ] }, "GridColumnIndex": 0 }, { "Label": "Pos27", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.44], "Focus": [17741.7], "XYStage": [ 22428, -116.80000000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos28", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.46], "Focus": [17741.7], "XYStage": [ 16364.900000000001, -181.10000000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos29", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.48000000000002], "Focus": [17741.5], "XYStage": [ 15712.1, -180 ] }, "GridColumnIndex": 0 }, { "Label": "Pos30", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.45000000000002], "Focus": [17741.7], "XYStage": [ 10346.1, 77.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos31", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.49], "Focus": [17741.4], "XYStage": [ 9755.5, 85.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos32", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.475], "Focus": [17741.5], "XYStage": [ 4775.5, 293.90000000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos33", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.445], "Focus": [17741.4], "XYStage": [ 3954.9, 293.90000000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos34", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.455], "Focus": [17741.4], "XYStage": [ -1324.9, 545.6 ] }, "GridColumnIndex": 0 }, { "Label": "Pos35", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.445], "Focus": [17741.3], "XYStage": [ -2097, 576.6 ] }, "GridColumnIndex": 0 } ], "z-step_um": 10, "GridRow": 0, "BitDepth": 16, "Depth": 2, "Channels": 6, "Source": "Micro-Manager", "ChColors": [ -16711681, -256, -65281, -1, -1, -16776961 ], "MetadataVersion": 10, "KeepShutterOpenChannels": true, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536, 65536 ], "CustomIntervals_ms": [], "Height": 1200, "MicroManagerVersion": "1.4.23 20180219", "GridColumn": 0, "UUID": "14a13bf7-c944-4672-b873-fcfbbb7ea13d", "PixelType": "GRAY16", "Width": 1200, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\Yan\\20191218-YC113-S7S8-miseq", "Frames": 1, "Comment": "", "UserName": "Nikon", "PixelSize_um": 1.1, "ROI": [ 0, 0, 1200, 1200 ], "Date": "2019-12-18", "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC", "P-DAPI-high" ], "KeepShutterOpenSlices": true, "IJType": 1, "ComputerName": "ZadorConfocal2", "Positions": 36, "PositionIndex": 9, "CameraTimeout": 20000, "TimeFirst": false }, "640 AO-Name": "AnalogIO", "Vincent-D3-Command": "Close" }, "FrameKey-0-1-1": { "COM4-StopBits": "1", "528 AO-MaxVolts": "5.0000", "XLIGHT Excitation Wheel-State": "1", "470 Shutter-Name": "AnalogIO", "Core-Shutter": "555", "555 AO-Demo": "No", "XLIGHT Dichroic-State": "2", "XYStage-StepSize-Y": "0.1000", "ZPositionUm": 208.82, "XYStage-StepSize-X": "0.1000", "PRIME-FirmwareVersion": "20.28", "PRIME-ClearCycles": "2", "520-Name": "DA Shutter", "405 Shutter-Name": "AnalogIO", "PIEZO-StepSize": "0.0050", "PRIME-ClearMode": "Pre-Exposure", "470 AO-MinVolts": "0.0000", "405 AO-SupportsTriggering": "1", "CameraChannelIndex": 0, "XYStage-StartSpeed": "500.0000", "COM9-StopBits": "2", "405 AO-Description": "NI DAC", "COM4-DelayBetweenCharsMs": "0.0000", "445 AO-Block voltage": "false", "520 AO-TriggerInputLine": "/Dev1/PFI0", "555 AO-Description": "NI DAC", "520 AO-TriggerSampleRate": "100000", "COM4-Verbose": "1", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM4-Name": "COM4", "405 Shutter-Volts": "0.0000", "Vincent-D3-Port": "COM1", "PRIME-PP 4 MIN ADU AFFECTED": "200", "470 Shutter-MinVolts": "0.0000", "PRIME-PP 3 ENABLED": "Yes", "470 AO-Description": "NI DAC", "PRIME-Description": "PVCAM API device adapter", "520 Shutter-MaxVolts": "5.0000", "520 Shutter-Volts": "0.0000", "528 AO-Sequenceable": "1", "PRIME-Binning": "1x1", "555 Shutter-MaxVolts": "5.0000", "528 Shutter-TriggerSequenceLength": "1000", "405 Shutter-Block voltage": "false", "XLIGHT Spin motor-State": "1", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "Vincent-D3-Time to open (ms)": "35.0000", "Vincent-D3-Time to close (ms)": "35.0000", "445 AO-TriggerSampleRate": "100000", "640 Shutter-IOChannel": "Dev1/ao14", "555 AO-Sequenceable": "1", "520 AO-Name": "AnalogIO", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "470 AO-Block voltage": "false", "COM1-Verbose": "1", "COM9-AnswerTimeout": "10000.0000", "528 Shutter-Name": "AnalogIO", "PRIME-Trigger Timeout (secs)": "10", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "NextFrame": 0, "470 AO-TriggerSequenceLength": "1000", "PRIME-TransposeXY": "0", "445 AO-TriggerSequenceLength": "1000", "COM1-Name": "COM1", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "COM9-DataBits": "8", "COM1-AnswerTimeout": "500.0000", "Camera": "", "TE2000-Version": "V2.50-V2.50-V2.10", "528 Shutter-Volts": "0.0000", "XLIGHT Hub-Name": "XLIGHT Hub", "PIEZO-Description": "Ludl stage driver adapter", "PositionName": "Pos9", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSampleRate": "100000", "PRIME-FanSpeedSetpoint": "High", "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "405 AO-TriggerSampleRate": "100000", "470 Shutter-Block voltage": "false", "470-State": "0", "COM3-Parity": "None", "405 AO-Volts": "0.0000", "PRIME-TransposeMirrorY": "0", "PRIME-ChipName": "GS144BSI", "470 AO-Volts": "0.0000", "PRIME-TransposeMirrorX": "0", "445 Shutter-SupportsTriggering": "0", "470 Shutter-SupportsTriggering": "0", "XLIGHT Hub-Description": "XLIGHT hub", "PRIME-PreampOffLimit": "10000", "470 AO-Sequenceable": "1", "528 Shutter-MaxVolts": "5.0000", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "Core-XYStage": "XYStage", "470 AO-TriggerSampleRate": "100000", "PRIME-Timing-PreTriggerDelayNs": "24672000.0000", "528-DA Device": "528 Shutter", "Focus-Description": "Nikon TE2000 focus adapter", "FilterBlock-State": "5", "520 AO-Demo": "No", "COM3-DelayBetweenCharsMs": "0.0000", "470 Shutter-Volts": "0.0000", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "PRIME-PP 2 THRESHOLD": "125", "PRIME-PP 4 THRESHOLD": "75", "XYStage-Acceleration": "4.0000", "470 Shutter-Demo": "No", "PRIME-Name": "PMPCIECam00", "640-DA Device": "640 Shutter", "405 AO-Block voltage": "false", "ElapsedTime-ms": 381852, "PRIME-Offset": "100", "445 AO-IOChannel": "Dev1/ao1", "640 AO-MaxVolts": "5.0000", "PositionIndex": 9, "PRIME-PP 1 ENABLED": "Yes", "COM1-BaudRate": "9600", "640-State": "0", "COM3-Verbose": "1", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "LudlController-TransmissionDelay": "0", "PRIME-FullWellCapacity": "62000", "PRIME-SMARTStreamingEnabled": "No", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "640 Shutter-Description": "NI DAC", "528-Description": "DA device that is used as a shutter", "COM4-Description": "Serial port driver (boost:asio)", "405 Shutter-Description": "NI DAC", "TE2000-Name": "TE2000", "528 Shutter-Description": "NI DAC", "COM9-Description": "Serial port driver (boost:asio)", "LudlController-Name": "LudlController", "445 Shutter-TriggerSequenceLength": "1000", "PRIME-SerialNumber": "A18B203008", "445 AO-Volts": "0.0000", "PRIME-X-dimension": "1200", "PRIME-ShutterCloseDelay": "0", "XLIGHT Disk slider-State": "2", "Position": "Default", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 AO-IOChannel": "Dev1/ao5", "520 AO-IOChannel": "Dev1/ao4", "528 AO-TriggerSequenceLength": "1000", "405 Shutter-MaxVolts": "5.0000", "470-DA Device": "470 Shutter", "PRIME-FTCapable": "No", "Core-ChannelGroup": "Channels", "555 AO-SupportsTriggering": "0", "PRIME-Y-dimension": "1200", "Frame": 0, "555 Shutter-Sequenceable": "1", "PRIME-Timing-ExposureTimeNs": "499998000.0000", "Core-ImageProcessor": "", "528-State": "0", "520 AO-Block voltage": "false", "XLIGHT Excitation Wheel-Label": "QUAD", "640 Shutter-Demo": "No", "COM3-StopBits": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "520 Shutter-IOChannel": "Dev1/ao11", "Width": 1200, "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-SupportsTriggering": "0", "640 AO-TriggerSampleRate": "100000", "520-Description": "DA device that is used as a shutter", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "405 Shutter-Sequenceable": "1", "405 Shutter-SupportsTriggering": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-TriggerSampleRate": "100000", "640 Shutter-MaxVolts": "5.0000", "555 AO-MaxVolts": "5.0000", "520 AO-TriggerSequenceLength": "1000", "COM3-BaudRate": "9600", "COM9-BaudRate": "9600", "Vincent-D3-Channel #": "Ch. #1", "PRIME-PMode": "Normal", "OpticalPath-Label": "Left(100)", "BitDepth": 16, "555 AO-TriggerSequenceLength": "1000", "445 Shutter-Description": "NI DAC", "405 AO-Sequenceable": "1", "PRIME-AcquisitionMethod": "Callbacks", "555 AO-Name": "AnalogIO", "PRIME-ShutterMode": "Pre-Exposure", "COM3-Handshaking": "Off", "445 AO-SupportsTriggering": "0", "ROI": "0-0-1200-1200", "COM9-Verbose": "1", "PRIME-Color": "OFF", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "PRIME-ShutterOpenDelay": "0", "COM1-Parity": "None", "Slice": 1, "SlicePosition": -40, "COM9-DelayBetweenCharsMs": "0.0000", "555-Name": "DA Shutter", "PIEZO-LudlSingleAxisName": "B", "XLIGHT Emission Wheel-Label": "585/11(T)", "640 Shutter-Name": "AnalogIO", "640 AO-Sequenceable": "1", "520 Shutter-Demo": "No", "528 Shutter-Demo": "No", "470-Name": "DA Shutter", "520-DA Device": "520 Shutter", "XYStage-Description": "Ludl XY stage driver adapter", "445 Shutter-MinVolts": "0.0000", "528 AO-TriggerSampleRate": "100000", "405 AO-MinVolts": "0.0000", "405 Shutter-TriggerSequenceLength": "1000", "Height": 1200, "555 AO-Block voltage": "false", "640 Shutter-Volts": "0.0000", "PixelType": "GRAY16", "445 Shutter-Block voltage": "false", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "520 Shutter-TriggerSequenceLength": "1000", "COM9-Name": "COM9", "Core-SLM": "", "555 AO-Volts": "4.5000", "555 Shutter-Block voltage": "false", "XYStage-Speed": "1800.0000", "520 AO-MaxVolts": "5.0000", "528 Shutter-SupportsTriggering": "0", "COM1-Description": "Serial port driver (boost:asio)", "PRIME-MetadataEnabled": "No", "520 Shutter-MinVolts": "0.0000", "470 Shutter-Sequenceable": "1", "PRIME-PreampDelay": "0", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-MinVolts": "0.0000", "ChannelIndex": 1, "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Core-AutoShutter": "1", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "Exposure-ms": 160, "PRIME-PP 2 ENABLED": "Yes", "640 Shutter-TriggerSampleRate": "100000", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "470 Shutter-IOChannel": "Dev1/ao10", "405 AO-Demo": "No", "PixelSizeUm": 1.1, "470 AO-Demo": "No", "470 Shutter-MaxVolts": "5.0000", "Vincent-D3-Name": "Vincent-D3", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FileName": "img_000000000_Il-T_001.tif", "LudlController-Reset": "Operate", "PRIME-Exposure": "160.00", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "445-Name": "DA Shutter", "555 Shutter-Name": "AnalogIO", "520 Shutter-TriggerSampleRate": "100000", "445 Shutter-IOChannel": "Dev1/ao9", "PRIME-TransposeCorrection": "0", "XYStage-StepSize": "0.1000", "445-Description": "DA device that is used as a shutter", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 AO-IOChannel": "Dev1/ao7", "520 Shutter-Name": "AnalogIO", "COM3-DataBits": "8", "640 AO-Volts": "0.0000", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "555 Shutter-IOChannel": "Dev1/ao13", "WaitInterval": null, "520 Shutter-Sequenceable": "1", "YPositionUm": 31506.2, "445-State": "0", "445-DA Device": "445 Shutter", "PRIME-CircularBufferAutoSize": "ON", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "555 AO-TriggerSampleRate": "100000", "445 Shutter-Volts": "0.0000", "XLIGHT Hub-Port": "COM3", "Core-AutoFocus": "", "COM4-Handshaking": "Off", "528 Shutter-MinVolts": "0.0000", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "445 Shutter-Name": "AnalogIO", "528 Shutter-Sequenceable": "1", "XYStage-TransposeMirrorX": "0", "XYStage-TransposeMirrorY": "0", "PRIME-ReadoutRate": "100MHz 16bit", "XYStage-Name": "Stage", "640 AO-SupportsTriggering": "0", "640-Name": "DA Shutter", "640 Shutter-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "640 AO-Block voltage": "false", "445 Shutter-Sequenceable": "1", "470 AO-MaxVolts": "5.0000", "COM4-BaudRate": "9600", "470-Description": "DA device that is used as a shutter", "520 Shutter-Description": "NI DAC", "PRIME-CCDTemperature": "-14.8500", "Time": "2019-12-18 21:36:49 -0500", "640 AO-TriggerSequenceLength": "1000", "470 AO-Name": "AnalogIO", "XLIGHT Disk slider-Label": "Disk Pos 40um", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "Binning": "1x1", "TE2000-ModelType": "1", "COM3-AnswerTimeout": "500.0000", "555 Shutter-SupportsTriggering": "0", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "TE2000-Port": "COM4", "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Description": "NI DAC", "COM4-AnswerTimeout": "500.0000", "COM1-DelayBetweenCharsMs": "0.0000", "FilterBlock-Label": "Confocal", "445 AO-Name": "AnalogIO", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-Timing-ReadoutTimeNs": "24672000.0000", "528 AO-Demo": "No", "520 AO-Sequenceable": "1", "555-Description": "DA device that is used as a shutter", "405-DA Device": "405 Shutter", "405 AO-TriggerSequenceLength": "1000", "640 AO-MinVolts": "0.0000", "640 Shutter-Block voltage": "false", "FrameIndex": 0, "PRIME-Port": "CMOS", "555 AO-MinVolts": "0.0000", "COM3-Description": "Serial port driver (boost:asio)", "528 AO-Description": "NI DAC", "555 AO-IOChannel": "Dev1/ao6", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "555 AO-TriggerInputLine": "/Dev1/PFI0", "PRIME-ActualInterval-ms": "390.5200", "LudlController-Port": "COM9", "445 Shutter-Demo": "No", "PRIME-Trigger-Expose Out-Mux": "1", "SliceIndex": 1, "PRIME-CCDTemperatureSetPoint": "-15.0000", "405 Shutter-MinVolts": "0.0000", "PRIME-ExposeOutMode": "First Row", "AxisPositions": null, "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Volts": "5.0000", "528-Name": "DA Shutter", "555-State": "1", "XLIGHT Dichroic-Label": "QUAD", "445 AO-Sequenceable": "1", "405-Name": "DA Shutter", "405 AO-IOChannel": "Dev1/ao0", "Core-Camera": "PRIME", "Focus-Name": "Focus", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "405 Shutter-IOChannel": "Dev1/ao8", "640 AO-Demo": "No", "640 Shutter-SupportsTriggering": "0", "445 AO-Demo": "No", "555-DA Device": "555 Shutter", "470 Shutter-Description": "NI DAC", "528 AO-Volts": "0.0000", "COM1-DataBits": "8", "Vincent-D3-Address": "x", "520 AO-SupportsTriggering": "0", "405-Description": "DA device that is used as a shutter", "640 Shutter-MinVolts": "0.0000", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "405 AO-Name": "AnalogIO", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "640 AO-TriggerInputLine": "/Dev1/PFI0", "405 AO-TriggerInputLine": "/Dev1/PFI0", "640-Description": "DA device that is used as a shutter", "445 Shutter-TriggerSampleRate": "100000", "470 Shutter-TriggerSequenceLength": "1000", "COM1-StopBits": "1", "520 Shutter-SupportsTriggering": "0", "405-State": "0", "Core-Initialize": "1", "XLIGHT Emission Wheel-State": "3", "520 AO-Description": "NI DAC", "528 AO-TriggerInputLine": "/Dev1/PFI0", "445 Shutter-MaxVolts": "5.0000", "COM9-Handshaking": "Off", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "XLIGHT Spin motor-Label": "On (spinning)", "PRIME-PP 5 ENABLED": "No", "UUID": "e3f30e34-fe0b-49a8-b2d6-91046b919be6", "PRIME-TriggerMode": "Internal Trigger", "COM1-Handshaking": "Off", "PRIME-Timing-PostTriggerDelayNs": "49344000.0000", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "Focus-Position": "17741.5000", "528 Shutter-Block voltage": "false", "Channel": "Il-T", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "555 Shutter-Demo": "No", "405 Shutter-Demo": "No", "528 AO-Name": "AnalogIO", "520-State": "0", "Core-Galvo": "", "405 Shutter-TriggerSampleRate": "100000", "Focus-FocusStepSize_(nm)": "100", "PRIME-PixelType": "16bit", "COM4-DataBits": "8", "470 AO-IOChannel": "Dev1/ao2", "520 AO-MinVolts": "0.0000", "COM9-Parity": "None", "PRIME-CircularBufferEnabled": "ON", "PRIME-PP 6 Reset": "No", "640 AO-Description": "NI DAC", "Source": "PRIME", "445 AO-MinVolts": "0.0000", "PIEZO-Name": "Stage", "445 AO-Description": "NI DAC", "PRIME-CircularBufferFrameCount": "46", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "520 AO-Volts": "0.0000", "528 Shutter-TriggerSampleRate": "100000", "XPositionUm": 19731.9, "OpticalPath-State": "4", "PRIME-PP 3 THRESHOLD": "80", "PRIME-PVCAM Version": "3.7.1", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Gain": "1-HDR", "640 Shutter-TriggerSequenceLength": "1000", "528 AO-SupportsTriggering": "0", "445 AO-TriggerInputLine": "/Dev1/PFI0", "OpticalPath-Name": "OpticalPath", "PRIME-PP 4 ENABLED": "Yes", "PRIME-PVCAM Adapter Version": "1.3.29", "COM3-Name": "COM3", "405 AO-MaxVolts": "5.0000", "520 Shutter-Block voltage": "false", "PRIME-Timing-ClearingTimeNs": "49344000.0000", "Core-Focus": "PIEZO", "528 AO-Block voltage": "false", "TE2000-Description": "Nikon TE2000 hub", "528 AO-MinVolts": "0.0000", "COM4-Parity": "None", "Summary": { "Slices": 11, "ChContrastMin": [ 0, 0, 0, 0, 0, 0 ], "PixelAspect": 1, "SlicesFirst": true, "Interval_ms": 0, "Time": "2019-12-18 21:36:42 -0500", "Prefix": "seq_4", "InitialPositionList": [ { "Label": "Pos0", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.21], "Focus": [17741.5], "XYStage": [ 44542.200000000004, 31636.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos1", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.22], "Focus": [17741.4], "XYStage": [ 43801.100000000006, 31637.9 ] }, "GridColumnIndex": 0 }, { "Label": "Pos2", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.5], "XYStage": [ 38561.3, 32040.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos3", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.185], "Focus": [17741.3], "XYStage": [ 37698.4, 32041.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos4", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.16], "Focus": [17741.5], "XYStage": [ 32375.4, 31758.9 ] }, "GridColumnIndex": 0 }, { "Label": "Pos5", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.17000000000002], "Focus": [17741.3], "XYStage": [ 31770.7, 31758.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos6", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.4], "XYStage": [ 26536.800000000003, 31068.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos7", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.205], "Focus": [17741.4], "XYStage": [ 25420.2, 31068.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos8", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.85], "Focus": [17741.3], "XYStage": [ 21059, 31508 ] }, "GridColumnIndex": 0 }, { "Label": "Pos9", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.82], "Focus": [17741.3], "XYStage": [ 19731.9, 31506.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos10", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.79], "Focus": [17741.4], "XYStage": [ 14528.5, 32007.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos11", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.83], "Focus": [17741.2], "XYStage": [ 13830.300000000001, 32009.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos12", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.79], "Focus": [17741.3], "XYStage": [ 8775.300000000001, 32017.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos13", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.815], "Focus": [17741.3], "XYStage": [ 8109.200000000001, 32008.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos14", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.805], "Focus": [17741.3], "XYStage": [ 3483.3, 31908.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos15", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.82], "Focus": [17741.2], "XYStage": [ 2686.8, 31903.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos16", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [211.48000000000002], "Focus": [17741.1], "XYStage": [ -1978.9, 31973.600000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos17", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [211.51500000000001], "Focus": [17741.1], "XYStage": [ -2957.8, 31982.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos18", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.195], "Focus": [17741.5], "XYStage": [ 47747.4, -576.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos19", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.23000000000002], "Focus": [17741.6], "XYStage": [ 47053.9, -576.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos20", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.205], "Focus": [17741.4], "XYStage": [ 41871.200000000004, -479.8 ] }, "GridColumnIndex": 0 }, { "Label": "Pos21", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.4], "XYStage": [ 40882.600000000006, -392 ] }, "GridColumnIndex": 0 }, { "Label": "Pos22", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.24], "Focus": [17741.4], "XYStage": [ 35905.6, -135.3 ] }, "GridColumnIndex": 0 }, { "Label": "Pos23", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.195], "Focus": [17741.5], "XYStage": [ 34990.9, -132.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos24", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.46], "Focus": [17741.4], "XYStage": [ 29678.100000000002, -958.1 ] }, "GridColumnIndex": 0 }, { "Label": "Pos25", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.44], "Focus": [17741.5], "XYStage": [ 28983.600000000002, -939.4000000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos26", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.475], "Focus": [17741.5], "XYStage": [ 23019.7, -122 ] }, "GridColumnIndex": 0 }, { "Label": "Pos27", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.44], "Focus": [17741.7], "XYStage": [ 22428, -116.80000000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos28", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.46], "Focus": [17741.7], "XYStage": [ 16364.900000000001, -181.10000000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos29", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.48000000000002], "Focus": [17741.5], "XYStage": [ 15712.1, -180 ] }, "GridColumnIndex": 0 }, { "Label": "Pos30", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.45000000000002], "Focus": [17741.7], "XYStage": [ 10346.1, 77.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos31", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.49], "Focus": [17741.4], "XYStage": [ 9755.5, 85.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos32", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.475], "Focus": [17741.5], "XYStage": [ 4775.5, 293.90000000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos33", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.445], "Focus": [17741.4], "XYStage": [ 3954.9, 293.90000000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos34", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.455], "Focus": [17741.4], "XYStage": [ -1324.9, 545.6 ] }, "GridColumnIndex": 0 }, { "Label": "Pos35", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.445], "Focus": [17741.3], "XYStage": [ -2097, 576.6 ] }, "GridColumnIndex": 0 } ], "z-step_um": 10, "GridRow": 0, "BitDepth": 16, "Depth": 2, "Channels": 6, "Source": "Micro-Manager", "ChColors": [ -16711681, -256, -65281, -1, -1, -16776961 ], "MetadataVersion": 10, "KeepShutterOpenChannels": true, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536, 65536 ], "CustomIntervals_ms": [], "Height": 1200, "MicroManagerVersion": "1.4.23 20180219", "GridColumn": 0, "UUID": "14a13bf7-c944-4672-b873-fcfbbb7ea13d", "PixelType": "GRAY16", "Width": 1200, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\Yan\\20191218-YC113-S7S8-miseq", "Frames": 1, "Comment": "", "UserName": "Nikon", "PixelSize_um": 1.1, "ROI": [ 0, 0, 1200, 1200 ], "Date": "2019-12-18", "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC", "P-DAPI-high" ], "KeepShutterOpenSlices": true, "IJType": 1, "ComputerName": "ZadorConfocal2", "Positions": 36, "PositionIndex": 9, "CameraTimeout": 20000, "TimeFirst": false }, "640 AO-Name": "AnalogIO", "Vincent-D3-Command": "Close" }, "FrameKey-0-1-2": { "COM4-StopBits": "1", "528 AO-MaxVolts": "5.0000", "XLIGHT Excitation Wheel-State": "1", "470 Shutter-Name": "AnalogIO", "Core-Shutter": "555", "555 AO-Demo": "No", "XLIGHT Dichroic-State": "2", "XYStage-StepSize-Y": "0.1000", "ZPositionUm": 218.82, "XYStage-StepSize-X": "0.1000", "PRIME-FirmwareVersion": "20.28", "PRIME-ClearCycles": "2", "520-Name": "DA Shutter", "405 Shutter-Name": "AnalogIO", "PIEZO-StepSize": "0.0050", "PRIME-ClearMode": "Pre-Exposure", "470 AO-MinVolts": "0.0000", "405 AO-SupportsTriggering": "1", "CameraChannelIndex": 0, "XYStage-StartSpeed": "500.0000", "COM9-StopBits": "2", "405 AO-Description": "NI DAC", "COM4-DelayBetweenCharsMs": "0.0000", "445 AO-Block voltage": "false", "520 AO-TriggerInputLine": "/Dev1/PFI0", "555 AO-Description": "NI DAC", "520 AO-TriggerSampleRate": "100000", "COM4-Verbose": "1", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM4-Name": "COM4", "405 Shutter-Volts": "0.0000", "Vincent-D3-Port": "COM1", "PRIME-PP 4 MIN ADU AFFECTED": "200", "470 Shutter-MinVolts": "0.0000", "PRIME-PP 3 ENABLED": "Yes", "470 AO-Description": "NI DAC", "PRIME-Description": "PVCAM API device adapter", "520 Shutter-MaxVolts": "5.0000", "520 Shutter-Volts": "0.0000", "528 AO-Sequenceable": "1", "PRIME-Binning": "1x1", "555 Shutter-MaxVolts": "5.0000", "528 Shutter-TriggerSequenceLength": "1000", "405 Shutter-Block voltage": "false", "XLIGHT Spin motor-State": "1", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "Vincent-D3-Time to open (ms)": "35.0000", "Vincent-D3-Time to close (ms)": "35.0000", "445 AO-TriggerSampleRate": "100000", "640 Shutter-IOChannel": "Dev1/ao14", "555 AO-Sequenceable": "1", "520 AO-Name": "AnalogIO", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "470 AO-Block voltage": "false", "COM1-Verbose": "1", "COM9-AnswerTimeout": "10000.0000", "528 Shutter-Name": "AnalogIO", "PRIME-Trigger Timeout (secs)": "10", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "NextFrame": 0, "470 AO-TriggerSequenceLength": "1000", "PRIME-TransposeXY": "0", "445 AO-TriggerSequenceLength": "1000", "COM1-Name": "COM1", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "COM9-DataBits": "8", "COM1-AnswerTimeout": "500.0000", "Camera": "", "TE2000-Version": "V2.50-V2.50-V2.10", "528 Shutter-Volts": "0.0000", "XLIGHT Hub-Name": "XLIGHT Hub", "PIEZO-Description": "Ludl stage driver adapter", "PositionName": "Pos9", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSampleRate": "100000", "PRIME-FanSpeedSetpoint": "High", "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "405 AO-TriggerSampleRate": "100000", "470 Shutter-Block voltage": "false", "470-State": "0", "COM3-Parity": "None", "405 AO-Volts": "0.0000", "PRIME-TransposeMirrorY": "0", "PRIME-ChipName": "GS144BSI", "470 AO-Volts": "0.0000", "PRIME-TransposeMirrorX": "0", "445 Shutter-SupportsTriggering": "0", "470 Shutter-SupportsTriggering": "0", "XLIGHT Hub-Description": "XLIGHT hub", "PRIME-PreampOffLimit": "10000", "470 AO-Sequenceable": "1", "528 Shutter-MaxVolts": "5.0000", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "Core-XYStage": "XYStage", "470 AO-TriggerSampleRate": "100000", "PRIME-Timing-PreTriggerDelayNs": "24672000.0000", "528-DA Device": "528 Shutter", "Focus-Description": "Nikon TE2000 focus adapter", "FilterBlock-State": "5", "520 AO-Demo": "No", "COM3-DelayBetweenCharsMs": "0.0000", "470 Shutter-Volts": "0.0000", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "PRIME-PP 2 THRESHOLD": "125", "PRIME-PP 4 THRESHOLD": "75", "XYStage-Acceleration": "4.0000", "470 Shutter-Demo": "No", "PRIME-Name": "PMPCIECam00", "640-DA Device": "640 Shutter", "405 AO-Block voltage": "false", "ElapsedTime-ms": 382299, "PRIME-Offset": "100", "445 AO-IOChannel": "Dev1/ao1", "640 AO-MaxVolts": "5.0000", "PositionIndex": 9, "PRIME-PP 1 ENABLED": "Yes", "COM1-BaudRate": "9600", "640-State": "0", "COM3-Verbose": "1", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "LudlController-TransmissionDelay": "0", "PRIME-FullWellCapacity": "62000", "PRIME-SMARTStreamingEnabled": "No", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "640 Shutter-Description": "NI DAC", "528-Description": "DA device that is used as a shutter", "COM4-Description": "Serial port driver (boost:asio)", "405 Shutter-Description": "NI DAC", "TE2000-Name": "TE2000", "528 Shutter-Description": "NI DAC", "COM9-Description": "Serial port driver (boost:asio)", "LudlController-Name": "LudlController", "445 Shutter-TriggerSequenceLength": "1000", "PRIME-SerialNumber": "A18B203008", "445 AO-Volts": "0.0000", "PRIME-X-dimension": "1200", "PRIME-ShutterCloseDelay": "0", "XLIGHT Disk slider-State": "2", "Position": "Default", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 AO-IOChannel": "Dev1/ao5", "520 AO-IOChannel": "Dev1/ao4", "528 AO-TriggerSequenceLength": "1000", "405 Shutter-MaxVolts": "5.0000", "470-DA Device": "470 Shutter", "PRIME-FTCapable": "No", "Core-ChannelGroup": "Channels", "555 AO-SupportsTriggering": "0", "PRIME-Y-dimension": "1200", "Frame": 0, "555 Shutter-Sequenceable": "1", "PRIME-Timing-ExposureTimeNs": "499998000.0000", "Core-ImageProcessor": "", "528-State": "0", "520 AO-Block voltage": "false", "XLIGHT Excitation Wheel-Label": "QUAD", "640 Shutter-Demo": "No", "COM3-StopBits": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "520 Shutter-IOChannel": "Dev1/ao11", "Width": 1200, "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-SupportsTriggering": "0", "640 AO-TriggerSampleRate": "100000", "520-Description": "DA device that is used as a shutter", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "405 Shutter-Sequenceable": "1", "405 Shutter-SupportsTriggering": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-TriggerSampleRate": "100000", "640 Shutter-MaxVolts": "5.0000", "555 AO-MaxVolts": "5.0000", "520 AO-TriggerSequenceLength": "1000", "COM3-BaudRate": "9600", "COM9-BaudRate": "9600", "Vincent-D3-Channel #": "Ch. #1", "PRIME-PMode": "Normal", "OpticalPath-Label": "Left(100)", "BitDepth": 16, "555 AO-TriggerSequenceLength": "1000", "445 Shutter-Description": "NI DAC", "405 AO-Sequenceable": "1", "PRIME-AcquisitionMethod": "Callbacks", "555 AO-Name": "AnalogIO", "PRIME-ShutterMode": "Pre-Exposure", "COM3-Handshaking": "Off", "445 AO-SupportsTriggering": "0", "ROI": "0-0-1200-1200", "COM9-Verbose": "1", "PRIME-Color": "OFF", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "PRIME-ShutterOpenDelay": "0", "COM1-Parity": "None", "Slice": 2, "SlicePosition": -30, "COM9-DelayBetweenCharsMs": "0.0000", "555-Name": "DA Shutter", "PIEZO-LudlSingleAxisName": "B", "XLIGHT Emission Wheel-Label": "585/11(T)", "640 Shutter-Name": "AnalogIO", "640 AO-Sequenceable": "1", "520 Shutter-Demo": "No", "528 Shutter-Demo": "No", "470-Name": "DA Shutter", "520-DA Device": "520 Shutter", "XYStage-Description": "Ludl XY stage driver adapter", "445 Shutter-MinVolts": "0.0000", "528 AO-TriggerSampleRate": "100000", "405 AO-MinVolts": "0.0000", "405 Shutter-TriggerSequenceLength": "1000", "Height": 1200, "555 AO-Block voltage": "false", "640 Shutter-Volts": "0.0000", "PixelType": "GRAY16", "445 Shutter-Block voltage": "false", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "520 Shutter-TriggerSequenceLength": "1000", "COM9-Name": "COM9", "Core-SLM": "", "555 AO-Volts": "4.5000", "555 Shutter-Block voltage": "false", "XYStage-Speed": "1800.0000", "520 AO-MaxVolts": "5.0000", "528 Shutter-SupportsTriggering": "0", "COM1-Description": "Serial port driver (boost:asio)", "PRIME-MetadataEnabled": "No", "520 Shutter-MinVolts": "0.0000", "470 Shutter-Sequenceable": "1", "PRIME-PreampDelay": "0", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-MinVolts": "0.0000", "ChannelIndex": 1, "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Core-AutoShutter": "1", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "Exposure-ms": 160, "PRIME-PP 2 ENABLED": "Yes", "640 Shutter-TriggerSampleRate": "100000", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "470 Shutter-IOChannel": "Dev1/ao10", "405 AO-Demo": "No", "PixelSizeUm": 1.1, "470 AO-Demo": "No", "470 Shutter-MaxVolts": "5.0000", "Vincent-D3-Name": "Vincent-D3", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FileName": "img_000000000_Il-T_002.tif", "LudlController-Reset": "Operate", "PRIME-Exposure": "160.00", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "445-Name": "DA Shutter", "555 Shutter-Name": "AnalogIO", "520 Shutter-TriggerSampleRate": "100000", "445 Shutter-IOChannel": "Dev1/ao9", "PRIME-TransposeCorrection": "0", "XYStage-StepSize": "0.1000", "445-Description": "DA device that is used as a shutter", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 AO-IOChannel": "Dev1/ao7", "520 Shutter-Name": "AnalogIO", "COM3-DataBits": "8", "640 AO-Volts": "0.0000", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "555 Shutter-IOChannel": "Dev1/ao13", "WaitInterval": null, "520 Shutter-Sequenceable": "1", "YPositionUm": 31506.2, "445-State": "0", "445-DA Device": "445 Shutter", "PRIME-CircularBufferAutoSize": "ON", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "555 AO-TriggerSampleRate": "100000", "445 Shutter-Volts": "0.0000", "XLIGHT Hub-Port": "COM3", "Core-AutoFocus": "", "COM4-Handshaking": "Off", "528 Shutter-MinVolts": "0.0000", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "445 Shutter-Name": "AnalogIO", "528 Shutter-Sequenceable": "1", "XYStage-TransposeMirrorX": "0", "XYStage-TransposeMirrorY": "0", "PRIME-ReadoutRate": "100MHz 16bit", "XYStage-Name": "Stage", "640 AO-SupportsTriggering": "0", "640-Name": "DA Shutter", "640 Shutter-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "640 AO-Block voltage": "false", "445 Shutter-Sequenceable": "1", "470 AO-MaxVolts": "5.0000", "COM4-BaudRate": "9600", "470-Description": "DA device that is used as a shutter", "520 Shutter-Description": "NI DAC", "PRIME-CCDTemperature": "-14.8500", "Time": "2019-12-18 21:36:50 -0500", "640 AO-TriggerSequenceLength": "1000", "470 AO-Name": "AnalogIO", "XLIGHT Disk slider-Label": "Disk Pos 40um", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "Binning": "1x1", "TE2000-ModelType": "1", "COM3-AnswerTimeout": "500.0000", "555 Shutter-SupportsTriggering": "0", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "TE2000-Port": "COM4", "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Description": "NI DAC", "COM4-AnswerTimeout": "500.0000", "COM1-DelayBetweenCharsMs": "0.0000", "FilterBlock-Label": "Confocal", "445 AO-Name": "AnalogIO", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-Timing-ReadoutTimeNs": "24672000.0000", "528 AO-Demo": "No", "520 AO-Sequenceable": "1", "555-Description": "DA device that is used as a shutter", "405-DA Device": "405 Shutter", "405 AO-TriggerSequenceLength": "1000", "640 AO-MinVolts": "0.0000", "640 Shutter-Block voltage": "false", "FrameIndex": 0, "PRIME-Port": "CMOS", "555 AO-MinVolts": "0.0000", "COM3-Description": "Serial port driver (boost:asio)", "528 AO-Description": "NI DAC", "555 AO-IOChannel": "Dev1/ao6", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "555 AO-TriggerInputLine": "/Dev1/PFI0", "PRIME-ActualInterval-ms": "390.5200", "LudlController-Port": "COM9", "445 Shutter-Demo": "No", "PRIME-Trigger-Expose Out-Mux": "1", "SliceIndex": 2, "PRIME-CCDTemperatureSetPoint": "-15.0000", "405 Shutter-MinVolts": "0.0000", "PRIME-ExposeOutMode": "First Row", "AxisPositions": null, "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Volts": "5.0000", "528-Name": "DA Shutter", "555-State": "1", "XLIGHT Dichroic-Label": "QUAD", "445 AO-Sequenceable": "1", "405-Name": "DA Shutter", "405 AO-IOChannel": "Dev1/ao0", "Core-Camera": "PRIME", "Focus-Name": "Focus", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "405 Shutter-IOChannel": "Dev1/ao8", "640 AO-Demo": "No", "640 Shutter-SupportsTriggering": "0", "445 AO-Demo": "No", "555-DA Device": "555 Shutter", "470 Shutter-Description": "NI DAC", "528 AO-Volts": "0.0000", "COM1-DataBits": "8", "Vincent-D3-Address": "x", "520 AO-SupportsTriggering": "0", "405-Description": "DA device that is used as a shutter", "640 Shutter-MinVolts": "0.0000", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "405 AO-Name": "AnalogIO", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "640 AO-TriggerInputLine": "/Dev1/PFI0", "405 AO-TriggerInputLine": "/Dev1/PFI0", "640-Description": "DA device that is used as a shutter", "445 Shutter-TriggerSampleRate": "100000", "470 Shutter-TriggerSequenceLength": "1000", "COM1-StopBits": "1", "520 Shutter-SupportsTriggering": "0", "405-State": "0", "Core-Initialize": "1", "XLIGHT Emission Wheel-State": "3", "520 AO-Description": "NI DAC", "528 AO-TriggerInputLine": "/Dev1/PFI0", "445 Shutter-MaxVolts": "5.0000", "COM9-Handshaking": "Off", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "XLIGHT Spin motor-Label": "On (spinning)", "PRIME-PP 5 ENABLED": "No", "UUID": "03982b12-e280-49ec-a096-84ead8ca07f5", "PRIME-TriggerMode": "Internal Trigger", "COM1-Handshaking": "Off", "PRIME-Timing-PostTriggerDelayNs": "49344000.0000", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "Focus-Position": "17741.5000", "528 Shutter-Block voltage": "false", "Channel": "Il-T", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "555 Shutter-Demo": "No", "405 Shutter-Demo": "No", "528 AO-Name": "AnalogIO", "520-State": "0", "Core-Galvo": "", "405 Shutter-TriggerSampleRate": "100000", "Focus-FocusStepSize_(nm)": "100", "PRIME-PixelType": "16bit", "COM4-DataBits": "8", "470 AO-IOChannel": "Dev1/ao2", "520 AO-MinVolts": "0.0000", "COM9-Parity": "None", "PRIME-CircularBufferEnabled": "ON", "PRIME-PP 6 Reset": "No", "640 AO-Description": "NI DAC", "Source": "PRIME", "445 AO-MinVolts": "0.0000", "PIEZO-Name": "Stage", "445 AO-Description": "NI DAC", "PRIME-CircularBufferFrameCount": "46", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "520 AO-Volts": "0.0000", "528 Shutter-TriggerSampleRate": "100000", "XPositionUm": 19731.9, "OpticalPath-State": "4", "PRIME-PP 3 THRESHOLD": "80", "PRIME-PVCAM Version": "3.7.1", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Gain": "1-HDR", "640 Shutter-TriggerSequenceLength": "1000", "528 AO-SupportsTriggering": "0", "445 AO-TriggerInputLine": "/Dev1/PFI0", "OpticalPath-Name": "OpticalPath", "PRIME-PP 4 ENABLED": "Yes", "PRIME-PVCAM Adapter Version": "1.3.29", "COM3-Name": "COM3", "405 AO-MaxVolts": "5.0000", "520 Shutter-Block voltage": "false", "PRIME-Timing-ClearingTimeNs": "49344000.0000", "Core-Focus": "PIEZO", "528 AO-Block voltage": "false", "TE2000-Description": "Nikon TE2000 hub", "528 AO-MinVolts": "0.0000", "COM4-Parity": "None", "Summary": { "Slices": 11, "ChContrastMin": [ 0, 0, 0, 0, 0, 0 ], "PixelAspect": 1, "SlicesFirst": true, "Interval_ms": 0, "Time": "2019-12-18 21:36:42 -0500", "Prefix": "seq_4", "InitialPositionList": [ { "Label": "Pos0", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.21], "Focus": [17741.5], "XYStage": [ 44542.200000000004, 31636.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos1", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.22], "Focus": [17741.4], "XYStage": [ 43801.100000000006, 31637.9 ] }, "GridColumnIndex": 0 }, { "Label": "Pos2", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.5], "XYStage": [ 38561.3, 32040.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos3", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.185], "Focus": [17741.3], "XYStage": [ 37698.4, 32041.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos4", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.16], "Focus": [17741.5], "XYStage": [ 32375.4, 31758.9 ] }, "GridColumnIndex": 0 }, { "Label": "Pos5", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.17000000000002], "Focus": [17741.3], "XYStage": [ 31770.7, 31758.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos6", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.4], "XYStage": [ 26536.800000000003, 31068.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos7", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.205], "Focus": [17741.4], "XYStage": [ 25420.2, 31068.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos8", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.85], "Focus": [17741.3], "XYStage": [ 21059, 31508 ] }, "GridColumnIndex": 0 }, { "Label": "Pos9", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.82], "Focus": [17741.3], "XYStage": [ 19731.9, 31506.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos10", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.79], "Focus": [17741.4], "XYStage": [ 14528.5, 32007.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos11", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.83], "Focus": [17741.2], "XYStage": [ 13830.300000000001, 32009.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos12", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.79], "Focus": [17741.3], "XYStage": [ 8775.300000000001, 32017.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos13", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.815], "Focus": [17741.3], "XYStage": [ 8109.200000000001, 32008.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos14", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.805], "Focus": [17741.3], "XYStage": [ 3483.3, 31908.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos15", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.82], "Focus": [17741.2], "XYStage": [ 2686.8, 31903.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos16", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [211.48000000000002], "Focus": [17741.1], "XYStage": [ -1978.9, 31973.600000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos17", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [211.51500000000001], "Focus": [17741.1], "XYStage": [ -2957.8, 31982.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos18", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.195], "Focus": [17741.5], "XYStage": [ 47747.4, -576.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos19", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.23000000000002], "Focus": [17741.6], "XYStage": [ 47053.9, -576.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos20", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.205], "Focus": [17741.4], "XYStage": [ 41871.200000000004, -479.8 ] }, "GridColumnIndex": 0 }, { "Label": "Pos21", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.4], "XYStage": [ 40882.600000000006, -392 ] }, "GridColumnIndex": 0 }, { "Label": "Pos22", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.24], "Focus": [17741.4], "XYStage": [ 35905.6, -135.3 ] }, "GridColumnIndex": 0 }, { "Label": "Pos23", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.195], "Focus": [17741.5], "XYStage": [ 34990.9, -132.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos24", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.46], "Focus": [17741.4], "XYStage": [ 29678.100000000002, -958.1 ] }, "GridColumnIndex": 0 }, { "Label": "Pos25", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.44], "Focus": [17741.5], "XYStage": [ 28983.600000000002, -939.4000000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos26", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.475], "Focus": [17741.5], "XYStage": [ 23019.7, -122 ] }, "GridColumnIndex": 0 }, { "Label": "Pos27", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.44], "Focus": [17741.7], "XYStage": [ 22428, -116.80000000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos28", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.46], "Focus": [17741.7], "XYStage": [ 16364.900000000001, -181.10000000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos29", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.48000000000002], "Focus": [17741.5], "XYStage": [ 15712.1, -180 ] }, "GridColumnIndex": 0 }, { "Label": "Pos30", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.45000000000002], "Focus": [17741.7], "XYStage": [ 10346.1, 77.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos31", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.49], "Focus": [17741.4], "XYStage": [ 9755.5, 85.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos32", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.475], "Focus": [17741.5], "XYStage": [ 4775.5, 293.90000000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos33", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.445], "Focus": [17741.4], "XYStage": [ 3954.9, 293.90000000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos34", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.455], "Focus": [17741.4], "XYStage": [ -1324.9, 545.6 ] }, "GridColumnIndex": 0 }, { "Label": "Pos35", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.445], "Focus": [17741.3], "XYStage": [ -2097, 576.6 ] }, "GridColumnIndex": 0 } ], "z-step_um": 10, "GridRow": 0, "BitDepth": 16, "Depth": 2, "Channels": 6, "Source": "Micro-Manager", "ChColors": [ -16711681, -256, -65281, -1, -1, -16776961 ], "MetadataVersion": 10, "KeepShutterOpenChannels": true, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536, 65536 ], "CustomIntervals_ms": [], "Height": 1200, "MicroManagerVersion": "1.4.23 20180219", "GridColumn": 0, "UUID": "14a13bf7-c944-4672-b873-fcfbbb7ea13d", "PixelType": "GRAY16", "Width": 1200, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\Yan\\20191218-YC113-S7S8-miseq", "Frames": 1, "Comment": "", "UserName": "Nikon", "PixelSize_um": 1.1, "ROI": [ 0, 0, 1200, 1200 ], "Date": "2019-12-18", "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC", "P-DAPI-high" ], "KeepShutterOpenSlices": true, "IJType": 1, "ComputerName": "ZadorConfocal2", "Positions": 36, "PositionIndex": 9, "CameraTimeout": 20000, "TimeFirst": false }, "640 AO-Name": "AnalogIO", "Vincent-D3-Command": "Close" }, "FrameKey-0-1-3": { "COM4-StopBits": "1", "528 AO-MaxVolts": "5.0000", "XLIGHT Excitation Wheel-State": "1", "470 Shutter-Name": "AnalogIO", "Core-Shutter": "555", "555 AO-Demo": "No", "XLIGHT Dichroic-State": "2", "XYStage-StepSize-Y": "0.1000", "ZPositionUm": 228.82, "XYStage-StepSize-X": "0.1000", "PRIME-FirmwareVersion": "20.28", "PRIME-ClearCycles": "2", "520-Name": "DA Shutter", "405 Shutter-Name": "AnalogIO", "PIEZO-StepSize": "0.0050", "PRIME-ClearMode": "Pre-Exposure", "470 AO-MinVolts": "0.0000", "405 AO-SupportsTriggering": "1", "CameraChannelIndex": 0, "XYStage-StartSpeed": "500.0000", "COM9-StopBits": "2", "405 AO-Description": "NI DAC", "COM4-DelayBetweenCharsMs": "0.0000", "445 AO-Block voltage": "false", "520 AO-TriggerInputLine": "/Dev1/PFI0", "555 AO-Description": "NI DAC", "520 AO-TriggerSampleRate": "100000", "COM4-Verbose": "1", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM4-Name": "COM4", "405 Shutter-Volts": "0.0000", "Vincent-D3-Port": "COM1", "PRIME-PP 4 MIN ADU AFFECTED": "200", "470 Shutter-MinVolts": "0.0000", "PRIME-PP 3 ENABLED": "Yes", "470 AO-Description": "NI DAC", "PRIME-Description": "PVCAM API device adapter", "520 Shutter-MaxVolts": "5.0000", "520 Shutter-Volts": "0.0000", "528 AO-Sequenceable": "1", "PRIME-Binning": "1x1", "555 Shutter-MaxVolts": "5.0000", "528 Shutter-TriggerSequenceLength": "1000", "405 Shutter-Block voltage": "false", "XLIGHT Spin motor-State": "1", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "Vincent-D3-Time to open (ms)": "35.0000", "Vincent-D3-Time to close (ms)": "35.0000", "445 AO-TriggerSampleRate": "100000", "640 Shutter-IOChannel": "Dev1/ao14", "555 AO-Sequenceable": "1", "520 AO-Name": "AnalogIO", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "470 AO-Block voltage": "false", "COM1-Verbose": "1", "COM9-AnswerTimeout": "10000.0000", "528 Shutter-Name": "AnalogIO", "PRIME-Trigger Timeout (secs)": "10", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "NextFrame": 0, "470 AO-TriggerSequenceLength": "1000", "PRIME-TransposeXY": "0", "445 AO-TriggerSequenceLength": "1000", "COM1-Name": "COM1", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "COM9-DataBits": "8", "COM1-AnswerTimeout": "500.0000", "Camera": "", "TE2000-Version": "V2.50-V2.50-V2.10", "528 Shutter-Volts": "0.0000", "XLIGHT Hub-Name": "XLIGHT Hub", "PIEZO-Description": "Ludl stage driver adapter", "PositionName": "Pos9", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSampleRate": "100000", "PRIME-FanSpeedSetpoint": "High", "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "405 AO-TriggerSampleRate": "100000", "470 Shutter-Block voltage": "false", "470-State": "0", "COM3-Parity": "None", "405 AO-Volts": "0.0000", "PRIME-TransposeMirrorY": "0", "PRIME-ChipName": "GS144BSI", "470 AO-Volts": "0.0000", "PRIME-TransposeMirrorX": "0", "445 Shutter-SupportsTriggering": "0", "470 Shutter-SupportsTriggering": "0", "XLIGHT Hub-Description": "XLIGHT hub", "PRIME-PreampOffLimit": "10000", "470 AO-Sequenceable": "1", "528 Shutter-MaxVolts": "5.0000", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "Core-XYStage": "XYStage", "470 AO-TriggerSampleRate": "100000", "PRIME-Timing-PreTriggerDelayNs": "24672000.0000", "528-DA Device": "528 Shutter", "Focus-Description": "Nikon TE2000 focus adapter", "FilterBlock-State": "5", "520 AO-Demo": "No", "COM3-DelayBetweenCharsMs": "0.0000", "470 Shutter-Volts": "0.0000", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "PRIME-PP 2 THRESHOLD": "125", "PRIME-PP 4 THRESHOLD": "75", "XYStage-Acceleration": "4.0000", "470 Shutter-Demo": "No", "PRIME-Name": "PMPCIECam00", "640-DA Device": "640 Shutter", "405 AO-Block voltage": "false", "ElapsedTime-ms": 382747, "PRIME-Offset": "100", "445 AO-IOChannel": "Dev1/ao1", "640 AO-MaxVolts": "5.0000", "PositionIndex": 9, "PRIME-PP 1 ENABLED": "Yes", "COM1-BaudRate": "9600", "640-State": "0", "COM3-Verbose": "1", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "LudlController-TransmissionDelay": "0", "PRIME-FullWellCapacity": "62000", "PRIME-SMARTStreamingEnabled": "No", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "640 Shutter-Description": "NI DAC", "528-Description": "DA device that is used as a shutter", "COM4-Description": "Serial port driver (boost:asio)", "405 Shutter-Description": "NI DAC", "TE2000-Name": "TE2000", "528 Shutter-Description": "NI DAC", "COM9-Description": "Serial port driver (boost:asio)", "LudlController-Name": "LudlController", "445 Shutter-TriggerSequenceLength": "1000", "PRIME-SerialNumber": "A18B203008", "445 AO-Volts": "0.0000", "PRIME-X-dimension": "1200", "PRIME-ShutterCloseDelay": "0", "XLIGHT Disk slider-State": "2", "Position": "Default", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 AO-IOChannel": "Dev1/ao5", "520 AO-IOChannel": "Dev1/ao4", "528 AO-TriggerSequenceLength": "1000", "405 Shutter-MaxVolts": "5.0000", "470-DA Device": "470 Shutter", "PRIME-FTCapable": "No", "Core-ChannelGroup": "Channels", "555 AO-SupportsTriggering": "0", "PRIME-Y-dimension": "1200", "Frame": 0, "555 Shutter-Sequenceable": "1", "PRIME-Timing-ExposureTimeNs": "499998000.0000", "Core-ImageProcessor": "", "528-State": "0", "520 AO-Block voltage": "false", "XLIGHT Excitation Wheel-Label": "QUAD", "640 Shutter-Demo": "No", "COM3-StopBits": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "520 Shutter-IOChannel": "Dev1/ao11", "Width": 1200, "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-SupportsTriggering": "0", "640 AO-TriggerSampleRate": "100000", "520-Description": "DA device that is used as a shutter", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "405 Shutter-Sequenceable": "1", "405 Shutter-SupportsTriggering": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-TriggerSampleRate": "100000", "640 Shutter-MaxVolts": "5.0000", "555 AO-MaxVolts": "5.0000", "520 AO-TriggerSequenceLength": "1000", "COM3-BaudRate": "9600", "COM9-BaudRate": "9600", "Vincent-D3-Channel #": "Ch. #1", "PRIME-PMode": "Normal", "OpticalPath-Label": "Left(100)", "BitDepth": 16, "555 AO-TriggerSequenceLength": "1000", "445 Shutter-Description": "NI DAC", "405 AO-Sequenceable": "1", "PRIME-AcquisitionMethod": "Callbacks", "555 AO-Name": "AnalogIO", "PRIME-ShutterMode": "Pre-Exposure", "COM3-Handshaking": "Off", "445 AO-SupportsTriggering": "0", "ROI": "0-0-1200-1200", "COM9-Verbose": "1", "PRIME-Color": "OFF", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "PRIME-ShutterOpenDelay": "0", "COM1-Parity": "None", "Slice": 3, "SlicePosition": -20, "COM9-DelayBetweenCharsMs": "0.0000", "555-Name": "DA Shutter", "PIEZO-LudlSingleAxisName": "B", "XLIGHT Emission Wheel-Label": "585/11(T)", "640 Shutter-Name": "AnalogIO", "640 AO-Sequenceable": "1", "520 Shutter-Demo": "No", "528 Shutter-Demo": "No", "470-Name": "DA Shutter", "520-DA Device": "520 Shutter", "XYStage-Description": "Ludl XY stage driver adapter", "445 Shutter-MinVolts": "0.0000", "528 AO-TriggerSampleRate": "100000", "405 AO-MinVolts": "0.0000", "405 Shutter-TriggerSequenceLength": "1000", "Height": 1200, "555 AO-Block voltage": "false", "640 Shutter-Volts": "0.0000", "PixelType": "GRAY16", "445 Shutter-Block voltage": "false", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "520 Shutter-TriggerSequenceLength": "1000", "COM9-Name": "COM9", "Core-SLM": "", "555 AO-Volts": "4.5000", "555 Shutter-Block voltage": "false", "XYStage-Speed": "1800.0000", "520 AO-MaxVolts": "5.0000", "528 Shutter-SupportsTriggering": "0", "COM1-Description": "Serial port driver (boost:asio)", "PRIME-MetadataEnabled": "No", "520 Shutter-MinVolts": "0.0000", "470 Shutter-Sequenceable": "1", "PRIME-PreampDelay": "0", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-MinVolts": "0.0000", "ChannelIndex": 1, "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Core-AutoShutter": "1", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "Exposure-ms": 160, "PRIME-PP 2 ENABLED": "Yes", "640 Shutter-TriggerSampleRate": "100000", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "470 Shutter-IOChannel": "Dev1/ao10", "405 AO-Demo": "No", "PixelSizeUm": 1.1, "470 AO-Demo": "No", "470 Shutter-MaxVolts": "5.0000", "Vincent-D3-Name": "Vincent-D3", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FileName": "img_000000000_Il-T_003.tif", "LudlController-Reset": "Operate", "PRIME-Exposure": "160.00", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "445-Name": "DA Shutter", "555 Shutter-Name": "AnalogIO", "520 Shutter-TriggerSampleRate": "100000", "445 Shutter-IOChannel": "Dev1/ao9", "PRIME-TransposeCorrection": "0", "XYStage-StepSize": "0.1000", "445-Description": "DA device that is used as a shutter", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 AO-IOChannel": "Dev1/ao7", "520 Shutter-Name": "AnalogIO", "COM3-DataBits": "8", "640 AO-Volts": "0.0000", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "555 Shutter-IOChannel": "Dev1/ao13", "WaitInterval": null, "520 Shutter-Sequenceable": "1", "YPositionUm": 31506.2, "445-State": "0", "445-DA Device": "445 Shutter", "PRIME-CircularBufferAutoSize": "ON", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "555 AO-TriggerSampleRate": "100000", "445 Shutter-Volts": "0.0000", "XLIGHT Hub-Port": "COM3", "Core-AutoFocus": "", "COM4-Handshaking": "Off", "528 Shutter-MinVolts": "0.0000", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "445 Shutter-Name": "AnalogIO", "528 Shutter-Sequenceable": "1", "XYStage-TransposeMirrorX": "0", "XYStage-TransposeMirrorY": "0", "PRIME-ReadoutRate": "100MHz 16bit", "XYStage-Name": "Stage", "640 AO-SupportsTriggering": "0", "640-Name": "DA Shutter", "640 Shutter-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "640 AO-Block voltage": "false", "445 Shutter-Sequenceable": "1", "470 AO-MaxVolts": "5.0000", "COM4-BaudRate": "9600", "470-Description": "DA device that is used as a shutter", "520 Shutter-Description": "NI DAC", "PRIME-CCDTemperature": "-14.8500", "Time": "2019-12-18 21:36:50 -0500", "640 AO-TriggerSequenceLength": "1000", "470 AO-Name": "AnalogIO", "XLIGHT Disk slider-Label": "Disk Pos 40um", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "Binning": "1x1", "TE2000-ModelType": "1", "COM3-AnswerTimeout": "500.0000", "555 Shutter-SupportsTriggering": "0", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "TE2000-Port": "COM4", "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Description": "NI DAC", "COM4-AnswerTimeout": "500.0000", "COM1-DelayBetweenCharsMs": "0.0000", "FilterBlock-Label": "Confocal", "445 AO-Name": "AnalogIO", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-Timing-ReadoutTimeNs": "24672000.0000", "528 AO-Demo": "No", "520 AO-Sequenceable": "1", "555-Description": "DA device that is used as a shutter", "405-DA Device": "405 Shutter", "405 AO-TriggerSequenceLength": "1000", "640 AO-MinVolts": "0.0000", "640 Shutter-Block voltage": "false", "FrameIndex": 0, "PRIME-Port": "CMOS", "555 AO-MinVolts": "0.0000", "COM3-Description": "Serial port driver (boost:asio)", "528 AO-Description": "NI DAC", "555 AO-IOChannel": "Dev1/ao6", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "555 AO-TriggerInputLine": "/Dev1/PFI0", "PRIME-ActualInterval-ms": "390.5200", "LudlController-Port": "COM9", "445 Shutter-Demo": "No", "PRIME-Trigger-Expose Out-Mux": "1", "SliceIndex": 3, "PRIME-CCDTemperatureSetPoint": "-15.0000", "405 Shutter-MinVolts": "0.0000", "PRIME-ExposeOutMode": "First Row", "AxisPositions": null, "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Volts": "5.0000", "528-Name": "DA Shutter", "555-State": "1", "XLIGHT Dichroic-Label": "QUAD", "445 AO-Sequenceable": "1", "405-Name": "DA Shutter", "405 AO-IOChannel": "Dev1/ao0", "Core-Camera": "PRIME", "Focus-Name": "Focus", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "405 Shutter-IOChannel": "Dev1/ao8", "640 AO-Demo": "No", "640 Shutter-SupportsTriggering": "0", "445 AO-Demo": "No", "555-DA Device": "555 Shutter", "470 Shutter-Description": "NI DAC", "528 AO-Volts": "0.0000", "COM1-DataBits": "8", "Vincent-D3-Address": "x", "520 AO-SupportsTriggering": "0", "405-Description": "DA device that is used as a shutter", "640 Shutter-MinVolts": "0.0000", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "405 AO-Name": "AnalogIO", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "640 AO-TriggerInputLine": "/Dev1/PFI0", "405 AO-TriggerInputLine": "/Dev1/PFI0", "640-Description": "DA device that is used as a shutter", "445 Shutter-TriggerSampleRate": "100000", "470 Shutter-TriggerSequenceLength": "1000", "COM1-StopBits": "1", "520 Shutter-SupportsTriggering": "0", "405-State": "0", "Core-Initialize": "1", "XLIGHT Emission Wheel-State": "3", "520 AO-Description": "NI DAC", "528 AO-TriggerInputLine": "/Dev1/PFI0", "445 Shutter-MaxVolts": "5.0000", "COM9-Handshaking": "Off", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "XLIGHT Spin motor-Label": "On (spinning)", "PRIME-PP 5 ENABLED": "No", "UUID": "5d1caa5f-2ac7-45d0-9791-3870b2286e73", "PRIME-TriggerMode": "Internal Trigger", "COM1-Handshaking": "Off", "PRIME-Timing-PostTriggerDelayNs": "49344000.0000", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "Focus-Position": "17741.5000", "528 Shutter-Block voltage": "false", "Channel": "Il-T", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "555 Shutter-Demo": "No", "405 Shutter-Demo": "No", "528 AO-Name": "AnalogIO", "520-State": "0", "Core-Galvo": "", "405 Shutter-TriggerSampleRate": "100000", "Focus-FocusStepSize_(nm)": "100", "PRIME-PixelType": "16bit", "COM4-DataBits": "8", "470 AO-IOChannel": "Dev1/ao2", "520 AO-MinVolts": "0.0000", "COM9-Parity": "None", "PRIME-CircularBufferEnabled": "ON", "PRIME-PP 6 Reset": "No", "640 AO-Description": "NI DAC", "Source": "PRIME", "445 AO-MinVolts": "0.0000", "PIEZO-Name": "Stage", "445 AO-Description": "NI DAC", "PRIME-CircularBufferFrameCount": "46", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "520 AO-Volts": "0.0000", "528 Shutter-TriggerSampleRate": "100000", "XPositionUm": 19731.9, "OpticalPath-State": "4", "PRIME-PP 3 THRESHOLD": "80", "PRIME-PVCAM Version": "3.7.1", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Gain": "1-HDR", "640 Shutter-TriggerSequenceLength": "1000", "528 AO-SupportsTriggering": "0", "445 AO-TriggerInputLine": "/Dev1/PFI0", "OpticalPath-Name": "OpticalPath", "PRIME-PP 4 ENABLED": "Yes", "PRIME-PVCAM Adapter Version": "1.3.29", "COM3-Name": "COM3", "405 AO-MaxVolts": "5.0000", "520 Shutter-Block voltage": "false", "PRIME-Timing-ClearingTimeNs": "49344000.0000", "Core-Focus": "PIEZO", "528 AO-Block voltage": "false", "TE2000-Description": "Nikon TE2000 hub", "528 AO-MinVolts": "0.0000", "COM4-Parity": "None", "Summary": { "Slices": 11, "ChContrastMin": [ 0, 0, 0, 0, 0, 0 ], "PixelAspect": 1, "SlicesFirst": true, "Interval_ms": 0, "Time": "2019-12-18 21:36:42 -0500", "Prefix": "seq_4", "InitialPositionList": [ { "Label": "Pos0", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.21], "Focus": [17741.5], "XYStage": [ 44542.200000000004, 31636.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos1", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.22], "Focus": [17741.4], "XYStage": [ 43801.100000000006, 31637.9 ] }, "GridColumnIndex": 0 }, { "Label": "Pos2", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.5], "XYStage": [ 38561.3, 32040.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos3", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.185], "Focus": [17741.3], "XYStage": [ 37698.4, 32041.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos4", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.16], "Focus": [17741.5], "XYStage": [ 32375.4, 31758.9 ] }, "GridColumnIndex": 0 }, { "Label": "Pos5", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.17000000000002], "Focus": [17741.3], "XYStage": [ 31770.7, 31758.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos6", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.4], "XYStage": [ 26536.800000000003, 31068.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos7", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.205], "Focus": [17741.4], "XYStage": [ 25420.2, 31068.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos8", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.85], "Focus": [17741.3], "XYStage": [ 21059, 31508 ] }, "GridColumnIndex": 0 }, { "Label": "Pos9", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.82], "Focus": [17741.3], "XYStage": [ 19731.9, 31506.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos10", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.79], "Focus": [17741.4], "XYStage": [ 14528.5, 32007.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos11", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.83], "Focus": [17741.2], "XYStage": [ 13830.300000000001, 32009.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos12", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.79], "Focus": [17741.3], "XYStage": [ 8775.300000000001, 32017.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos13", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.815], "Focus": [17741.3], "XYStage": [ 8109.200000000001, 32008.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos14", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.805], "Focus": [17741.3], "XYStage": [ 3483.3, 31908.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos15", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.82], "Focus": [17741.2], "XYStage": [ 2686.8, 31903.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos16", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [211.48000000000002], "Focus": [17741.1], "XYStage": [ -1978.9, 31973.600000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos17", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [211.51500000000001], "Focus": [17741.1], "XYStage": [ -2957.8, 31982.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos18", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.195], "Focus": [17741.5], "XYStage": [ 47747.4, -576.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos19", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.23000000000002], "Focus": [17741.6], "XYStage": [ 47053.9, -576.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos20", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.205], "Focus": [17741.4], "XYStage": [ 41871.200000000004, -479.8 ] }, "GridColumnIndex": 0 }, { "Label": "Pos21", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.4], "XYStage": [ 40882.600000000006, -392 ] }, "GridColumnIndex": 0 }, { "Label": "Pos22", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.24], "Focus": [17741.4], "XYStage": [ 35905.6, -135.3 ] }, "GridColumnIndex": 0 }, { "Label": "Pos23", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.195], "Focus": [17741.5], "XYStage": [ 34990.9, -132.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos24", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.46], "Focus": [17741.4], "XYStage": [ 29678.100000000002, -958.1 ] }, "GridColumnIndex": 0 }, { "Label": "Pos25", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.44], "Focus": [17741.5], "XYStage": [ 28983.600000000002, -939.4000000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos26", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.475], "Focus": [17741.5], "XYStage": [ 23019.7, -122 ] }, "GridColumnIndex": 0 }, { "Label": "Pos27", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.44], "Focus": [17741.7], "XYStage": [ 22428, -116.80000000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos28", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.46], "Focus": [17741.7], "XYStage": [ 16364.900000000001, -181.10000000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos29", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.48000000000002], "Focus": [17741.5], "XYStage": [ 15712.1, -180 ] }, "GridColumnIndex": 0 }, { "Label": "Pos30", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.45000000000002], "Focus": [17741.7], "XYStage": [ 10346.1, 77.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos31", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.49], "Focus": [17741.4], "XYStage": [ 9755.5, 85.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos32", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.475], "Focus": [17741.5], "XYStage": [ 4775.5, 293.90000000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos33", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.445], "Focus": [17741.4], "XYStage": [ 3954.9, 293.90000000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos34", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.455], "Focus": [17741.4], "XYStage": [ -1324.9, 545.6 ] }, "GridColumnIndex": 0 }, { "Label": "Pos35", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.445], "Focus": [17741.3], "XYStage": [ -2097, 576.6 ] }, "GridColumnIndex": 0 } ], "z-step_um": 10, "GridRow": 0, "BitDepth": 16, "Depth": 2, "Channels": 6, "Source": "Micro-Manager", "ChColors": [ -16711681, -256, -65281, -1, -1, -16776961 ], "MetadataVersion": 10, "KeepShutterOpenChannels": true, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536, 65536 ], "CustomIntervals_ms": [], "Height": 1200, "MicroManagerVersion": "1.4.23 20180219", "GridColumn": 0, "UUID": "14a13bf7-c944-4672-b873-fcfbbb7ea13d", "PixelType": "GRAY16", "Width": 1200, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\Yan\\20191218-YC113-S7S8-miseq", "Frames": 1, "Comment": "", "UserName": "Nikon", "PixelSize_um": 1.1, "ROI": [ 0, 0, 1200, 1200 ], "Date": "2019-12-18", "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC", "P-DAPI-high" ], "KeepShutterOpenSlices": true, "IJType": 1, "ComputerName": "ZadorConfocal2", "Positions": 36, "PositionIndex": 9, "CameraTimeout": 20000, "TimeFirst": false }, "640 AO-Name": "AnalogIO", "Vincent-D3-Command": "Close" }, "FrameKey-0-1-4": { "COM4-StopBits": "1", "528 AO-MaxVolts": "5.0000", "XLIGHT Excitation Wheel-State": "1", "470 Shutter-Name": "AnalogIO", "Core-Shutter": "555", "555 AO-Demo": "No", "XLIGHT Dichroic-State": "2", "XYStage-StepSize-Y": "0.1000", "ZPositionUm": 238.82, "XYStage-StepSize-X": "0.1000", "PRIME-FirmwareVersion": "20.28", "PRIME-ClearCycles": "2", "520-Name": "DA Shutter", "405 Shutter-Name": "AnalogIO", "PIEZO-StepSize": "0.0050", "PRIME-ClearMode": "Pre-Exposure", "470 AO-MinVolts": "0.0000", "405 AO-SupportsTriggering": "1", "CameraChannelIndex": 0, "XYStage-StartSpeed": "500.0000", "COM9-StopBits": "2", "405 AO-Description": "NI DAC", "COM4-DelayBetweenCharsMs": "0.0000", "445 AO-Block voltage": "false", "520 AO-TriggerInputLine": "/Dev1/PFI0", "555 AO-Description": "NI DAC", "520 AO-TriggerSampleRate": "100000", "COM4-Verbose": "1", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM4-Name": "COM4", "405 Shutter-Volts": "0.0000", "Vincent-D3-Port": "COM1", "PRIME-PP 4 MIN ADU AFFECTED": "200", "470 Shutter-MinVolts": "0.0000", "PRIME-PP 3 ENABLED": "Yes", "470 AO-Description": "NI DAC", "PRIME-Description": "PVCAM API device adapter", "520 Shutter-MaxVolts": "5.0000", "520 Shutter-Volts": "0.0000", "528 AO-Sequenceable": "1", "PRIME-Binning": "1x1", "555 Shutter-MaxVolts": "5.0000", "528 Shutter-TriggerSequenceLength": "1000", "405 Shutter-Block voltage": "false", "XLIGHT Spin motor-State": "1", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "Vincent-D3-Time to open (ms)": "35.0000", "Vincent-D3-Time to close (ms)": "35.0000", "445 AO-TriggerSampleRate": "100000", "640 Shutter-IOChannel": "Dev1/ao14", "555 AO-Sequenceable": "1", "520 AO-Name": "AnalogIO", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "470 AO-Block voltage": "false", "COM1-Verbose": "1", "COM9-AnswerTimeout": "10000.0000", "528 Shutter-Name": "AnalogIO", "PRIME-Trigger Timeout (secs)": "10", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "NextFrame": 0, "470 AO-TriggerSequenceLength": "1000", "PRIME-TransposeXY": "0", "445 AO-TriggerSequenceLength": "1000", "COM1-Name": "COM1", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "COM9-DataBits": "8", "COM1-AnswerTimeout": "500.0000", "Camera": "", "TE2000-Version": "V2.50-V2.50-V2.10", "528 Shutter-Volts": "0.0000", "XLIGHT Hub-Name": "XLIGHT Hub", "PIEZO-Description": "Ludl stage driver adapter", "PositionName": "Pos9", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSampleRate": "100000", "PRIME-FanSpeedSetpoint": "High", "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "405 AO-TriggerSampleRate": "100000", "470 Shutter-Block voltage": "false", "470-State": "0", "COM3-Parity": "None", "405 AO-Volts": "0.0000", "PRIME-TransposeMirrorY": "0", "PRIME-ChipName": "GS144BSI", "470 AO-Volts": "0.0000", "PRIME-TransposeMirrorX": "0", "445 Shutter-SupportsTriggering": "0", "470 Shutter-SupportsTriggering": "0", "XLIGHT Hub-Description": "XLIGHT hub", "PRIME-PreampOffLimit": "10000", "470 AO-Sequenceable": "1", "528 Shutter-MaxVolts": "5.0000", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "Core-XYStage": "XYStage", "470 AO-TriggerSampleRate": "100000", "PRIME-Timing-PreTriggerDelayNs": "24672000.0000", "528-DA Device": "528 Shutter", "Focus-Description": "Nikon TE2000 focus adapter", "FilterBlock-State": "5", "520 AO-Demo": "No", "COM3-DelayBetweenCharsMs": "0.0000", "470 Shutter-Volts": "0.0000", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "PRIME-PP 2 THRESHOLD": "125", "PRIME-PP 4 THRESHOLD": "75", "XYStage-Acceleration": "4.0000", "470 Shutter-Demo": "No", "PRIME-Name": "PMPCIECam00", "640-DA Device": "640 Shutter", "405 AO-Block voltage": "false", "ElapsedTime-ms": 383194, "PRIME-Offset": "100", "445 AO-IOChannel": "Dev1/ao1", "640 AO-MaxVolts": "5.0000", "PositionIndex": 9, "PRIME-PP 1 ENABLED": "Yes", "COM1-BaudRate": "9600", "640-State": "0", "COM3-Verbose": "1", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "LudlController-TransmissionDelay": "0", "PRIME-FullWellCapacity": "62000", "PRIME-SMARTStreamingEnabled": "No", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "640 Shutter-Description": "NI DAC", "528-Description": "DA device that is used as a shutter", "COM4-Description": "Serial port driver (boost:asio)", "405 Shutter-Description": "NI DAC", "TE2000-Name": "TE2000", "528 Shutter-Description": "NI DAC", "COM9-Description": "Serial port driver (boost:asio)", "LudlController-Name": "LudlController", "445 Shutter-TriggerSequenceLength": "1000", "PRIME-SerialNumber": "A18B203008", "445 AO-Volts": "0.0000", "PRIME-X-dimension": "1200", "PRIME-ShutterCloseDelay": "0", "XLIGHT Disk slider-State": "2", "Position": "Default", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 AO-IOChannel": "Dev1/ao5", "520 AO-IOChannel": "Dev1/ao4", "528 AO-TriggerSequenceLength": "1000", "405 Shutter-MaxVolts": "5.0000", "470-DA Device": "470 Shutter", "PRIME-FTCapable": "No", "Core-ChannelGroup": "Channels", "555 AO-SupportsTriggering": "0", "PRIME-Y-dimension": "1200", "Frame": 0, "555 Shutter-Sequenceable": "1", "PRIME-Timing-ExposureTimeNs": "499998000.0000", "Core-ImageProcessor": "", "528-State": "0", "520 AO-Block voltage": "false", "XLIGHT Excitation Wheel-Label": "QUAD", "640 Shutter-Demo": "No", "COM3-StopBits": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "520 Shutter-IOChannel": "Dev1/ao11", "Width": 1200, "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-SupportsTriggering": "0", "640 AO-TriggerSampleRate": "100000", "520-Description": "DA device that is used as a shutter", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "405 Shutter-Sequenceable": "1", "405 Shutter-SupportsTriggering": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-TriggerSampleRate": "100000", "640 Shutter-MaxVolts": "5.0000", "555 AO-MaxVolts": "5.0000", "520 AO-TriggerSequenceLength": "1000", "COM3-BaudRate": "9600", "COM9-BaudRate": "9600", "Vincent-D3-Channel #": "Ch. #1", "PRIME-PMode": "Normal", "OpticalPath-Label": "Left(100)", "BitDepth": 16, "555 AO-TriggerSequenceLength": "1000", "445 Shutter-Description": "NI DAC", "405 AO-Sequenceable": "1", "PRIME-AcquisitionMethod": "Callbacks", "555 AO-Name": "AnalogIO", "PRIME-ShutterMode": "Pre-Exposure", "COM3-Handshaking": "Off", "445 AO-SupportsTriggering": "0", "ROI": "0-0-1200-1200", "COM9-Verbose": "1", "PRIME-Color": "OFF", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "PRIME-ShutterOpenDelay": "0", "COM1-Parity": "None", "Slice": 4, "SlicePosition": -10, "COM9-DelayBetweenCharsMs": "0.0000", "555-Name": "DA Shutter", "PIEZO-LudlSingleAxisName": "B", "XLIGHT Emission Wheel-Label": "585/11(T)", "640 Shutter-Name": "AnalogIO", "640 AO-Sequenceable": "1", "520 Shutter-Demo": "No", "528 Shutter-Demo": "No", "470-Name": "DA Shutter", "520-DA Device": "520 Shutter", "XYStage-Description": "Ludl XY stage driver adapter", "445 Shutter-MinVolts": "0.0000", "528 AO-TriggerSampleRate": "100000", "405 AO-MinVolts": "0.0000", "405 Shutter-TriggerSequenceLength": "1000", "Height": 1200, "555 AO-Block voltage": "false", "640 Shutter-Volts": "0.0000", "PixelType": "GRAY16", "445 Shutter-Block voltage": "false", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "520 Shutter-TriggerSequenceLength": "1000", "COM9-Name": "COM9", "Core-SLM": "", "555 AO-Volts": "4.5000", "555 Shutter-Block voltage": "false", "XYStage-Speed": "1800.0000", "520 AO-MaxVolts": "5.0000", "528 Shutter-SupportsTriggering": "0", "COM1-Description": "Serial port driver (boost:asio)", "PRIME-MetadataEnabled": "No", "520 Shutter-MinVolts": "0.0000", "470 Shutter-Sequenceable": "1", "PRIME-PreampDelay": "0", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-MinVolts": "0.0000", "ChannelIndex": 1, "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Core-AutoShutter": "1", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "Exposure-ms": 160, "PRIME-PP 2 ENABLED": "Yes", "640 Shutter-TriggerSampleRate": "100000", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "470 Shutter-IOChannel": "Dev1/ao10", "405 AO-Demo": "No", "PixelSizeUm": 1.1, "470 AO-Demo": "No", "470 Shutter-MaxVolts": "5.0000", "Vincent-D3-Name": "Vincent-D3", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FileName": "img_000000000_Il-T_004.tif", "LudlController-Reset": "Operate", "PRIME-Exposure": "160.00", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "445-Name": "DA Shutter", "555 Shutter-Name": "AnalogIO", "520 Shutter-TriggerSampleRate": "100000", "445 Shutter-IOChannel": "Dev1/ao9", "PRIME-TransposeCorrection": "0", "XYStage-StepSize": "0.1000", "445-Description": "DA device that is used as a shutter", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 AO-IOChannel": "Dev1/ao7", "520 Shutter-Name": "AnalogIO", "COM3-DataBits": "8", "640 AO-Volts": "0.0000", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "555 Shutter-IOChannel": "Dev1/ao13", "WaitInterval": null, "520 Shutter-Sequenceable": "1", "YPositionUm": 31506.2, "445-State": "0", "445-DA Device": "445 Shutter", "PRIME-CircularBufferAutoSize": "ON", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "555 AO-TriggerSampleRate": "100000", "445 Shutter-Volts": "0.0000", "XLIGHT Hub-Port": "COM3", "Core-AutoFocus": "", "COM4-Handshaking": "Off", "528 Shutter-MinVolts": "0.0000", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "445 Shutter-Name": "AnalogIO", "528 Shutter-Sequenceable": "1", "XYStage-TransposeMirrorX": "0", "XYStage-TransposeMirrorY": "0", "PRIME-ReadoutRate": "100MHz 16bit", "XYStage-Name": "Stage", "640 AO-SupportsTriggering": "0", "640-Name": "DA Shutter", "640 Shutter-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "640 AO-Block voltage": "false", "445 Shutter-Sequenceable": "1", "470 AO-MaxVolts": "5.0000", "COM4-BaudRate": "9600", "470-Description": "DA device that is used as a shutter", "520 Shutter-Description": "NI DAC", "PRIME-CCDTemperature": "-14.8500", "Time": "2019-12-18 21:36:51 -0500", "640 AO-TriggerSequenceLength": "1000", "470 AO-Name": "AnalogIO", "XLIGHT Disk slider-Label": "Disk Pos 40um", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "Binning": "1x1", "TE2000-ModelType": "1", "COM3-AnswerTimeout": "500.0000", "555 Shutter-SupportsTriggering": "0", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "TE2000-Port": "COM4", "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Description": "NI DAC", "COM4-AnswerTimeout": "500.0000", "COM1-DelayBetweenCharsMs": "0.0000", "FilterBlock-Label": "Confocal", "445 AO-Name": "AnalogIO", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-Timing-ReadoutTimeNs": "24672000.0000", "528 AO-Demo": "No", "520 AO-Sequenceable": "1", "555-Description": "DA device that is used as a shutter", "405-DA Device": "405 Shutter", "405 AO-TriggerSequenceLength": "1000", "640 AO-MinVolts": "0.0000", "640 Shutter-Block voltage": "false", "FrameIndex": 0, "PRIME-Port": "CMOS", "555 AO-MinVolts": "0.0000", "COM3-Description": "Serial port driver (boost:asio)", "528 AO-Description": "NI DAC", "555 AO-IOChannel": "Dev1/ao6", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "555 AO-TriggerInputLine": "/Dev1/PFI0", "PRIME-ActualInterval-ms": "390.5200", "LudlController-Port": "COM9", "445 Shutter-Demo": "No", "PRIME-Trigger-Expose Out-Mux": "1", "SliceIndex": 4, "PRIME-CCDTemperatureSetPoint": "-15.0000", "405 Shutter-MinVolts": "0.0000", "PRIME-ExposeOutMode": "First Row", "AxisPositions": null, "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Volts": "5.0000", "528-Name": "DA Shutter", "555-State": "1", "XLIGHT Dichroic-Label": "QUAD", "445 AO-Sequenceable": "1", "405-Name": "DA Shutter", "405 AO-IOChannel": "Dev1/ao0", "Core-Camera": "PRIME", "Focus-Name": "Focus", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "405 Shutter-IOChannel": "Dev1/ao8", "640 AO-Demo": "No", "640 Shutter-SupportsTriggering": "0", "445 AO-Demo": "No", "555-DA Device": "555 Shutter", "470 Shutter-Description": "NI DAC", "528 AO-Volts": "0.0000", "COM1-DataBits": "8", "Vincent-D3-Address": "x", "520 AO-SupportsTriggering": "0", "405-Description": "DA device that is used as a shutter", "640 Shutter-MinVolts": "0.0000", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "405 AO-Name": "AnalogIO", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "640 AO-TriggerInputLine": "/Dev1/PFI0", "405 AO-TriggerInputLine": "/Dev1/PFI0", "640-Description": "DA device that is used as a shutter", "445 Shutter-TriggerSampleRate": "100000", "470 Shutter-TriggerSequenceLength": "1000", "COM1-StopBits": "1", "520 Shutter-SupportsTriggering": "0", "405-State": "0", "Core-Initialize": "1", "XLIGHT Emission Wheel-State": "3", "520 AO-Description": "NI DAC", "528 AO-TriggerInputLine": "/Dev1/PFI0", "445 Shutter-MaxVolts": "5.0000", "COM9-Handshaking": "Off", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "XLIGHT Spin motor-Label": "On (spinning)", "PRIME-PP 5 ENABLED": "No", "UUID": "f65cb27a-7c06-4624-ae38-f77207d11dbc", "PRIME-TriggerMode": "Internal Trigger", "COM1-Handshaking": "Off", "PRIME-Timing-PostTriggerDelayNs": "49344000.0000", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "Focus-Position": "17741.5000", "528 Shutter-Block voltage": "false", "Channel": "Il-T", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "555 Shutter-Demo": "No", "405 Shutter-Demo": "No", "528 AO-Name": "AnalogIO", "520-State": "0", "Core-Galvo": "", "405 Shutter-TriggerSampleRate": "100000", "Focus-FocusStepSize_(nm)": "100", "PRIME-PixelType": "16bit", "COM4-DataBits": "8", "470 AO-IOChannel": "Dev1/ao2", "520 AO-MinVolts": "0.0000", "COM9-Parity": "None", "PRIME-CircularBufferEnabled": "ON", "PRIME-PP 6 Reset": "No", "640 AO-Description": "NI DAC", "Source": "PRIME", "445 AO-MinVolts": "0.0000", "PIEZO-Name": "Stage", "445 AO-Description": "NI DAC", "PRIME-CircularBufferFrameCount": "46", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "520 AO-Volts": "0.0000", "528 Shutter-TriggerSampleRate": "100000", "XPositionUm": 19731.9, "OpticalPath-State": "4", "PRIME-PP 3 THRESHOLD": "80", "PRIME-PVCAM Version": "3.7.1", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Gain": "1-HDR", "640 Shutter-TriggerSequenceLength": "1000", "528 AO-SupportsTriggering": "0", "445 AO-TriggerInputLine": "/Dev1/PFI0", "OpticalPath-Name": "OpticalPath", "PRIME-PP 4 ENABLED": "Yes", "PRIME-PVCAM Adapter Version": "1.3.29", "COM3-Name": "COM3", "405 AO-MaxVolts": "5.0000", "520 Shutter-Block voltage": "false", "PRIME-Timing-ClearingTimeNs": "49344000.0000", "Core-Focus": "PIEZO", "528 AO-Block voltage": "false", "TE2000-Description": "Nikon TE2000 hub", "528 AO-MinVolts": "0.0000", "COM4-Parity": "None", "Summary": { "Slices": 11, "ChContrastMin": [ 0, 0, 0, 0, 0, 0 ], "PixelAspect": 1, "SlicesFirst": true, "Interval_ms": 0, "Time": "2019-12-18 21:36:42 -0500", "Prefix": "seq_4", "InitialPositionList": [ { "Label": "Pos0", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.21], "Focus": [17741.5], "XYStage": [ 44542.200000000004, 31636.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos1", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.22], "Focus": [17741.4], "XYStage": [ 43801.100000000006, 31637.9 ] }, "GridColumnIndex": 0 }, { "Label": "Pos2", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.5], "XYStage": [ 38561.3, 32040.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos3", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.185], "Focus": [17741.3], "XYStage": [ 37698.4, 32041.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos4", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.16], "Focus": [17741.5], "XYStage": [ 32375.4, 31758.9 ] }, "GridColumnIndex": 0 }, { "Label": "Pos5", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.17000000000002], "Focus": [17741.3], "XYStage": [ 31770.7, 31758.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos6", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.4], "XYStage": [ 26536.800000000003, 31068.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos7", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.205], "Focus": [17741.4], "XYStage": [ 25420.2, 31068.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos8", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.85], "Focus": [17741.3], "XYStage": [ 21059, 31508 ] }, "GridColumnIndex": 0 }, { "Label": "Pos9", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.82], "Focus": [17741.3], "XYStage": [ 19731.9, 31506.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos10", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.79], "Focus": [17741.4], "XYStage": [ 14528.5, 32007.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos11", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.83], "Focus": [17741.2], "XYStage": [ 13830.300000000001, 32009.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos12", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.79], "Focus": [17741.3], "XYStage": [ 8775.300000000001, 32017.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos13", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.815], "Focus": [17741.3], "XYStage": [ 8109.200000000001, 32008.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos14", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.805], "Focus": [17741.3], "XYStage": [ 3483.3, 31908.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos15", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.82], "Focus": [17741.2], "XYStage": [ 2686.8, 31903.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos16", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [211.48000000000002], "Focus": [17741.1], "XYStage": [ -1978.9, 31973.600000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos17", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [211.51500000000001], "Focus": [17741.1], "XYStage": [ -2957.8, 31982.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos18", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.195], "Focus": [17741.5], "XYStage": [ 47747.4, -576.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos19", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.23000000000002], "Focus": [17741.6], "XYStage": [ 47053.9, -576.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos20", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.205], "Focus": [17741.4], "XYStage": [ 41871.200000000004, -479.8 ] }, "GridColumnIndex": 0 }, { "Label": "Pos21", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.4], "XYStage": [ 40882.600000000006, -392 ] }, "GridColumnIndex": 0 }, { "Label": "Pos22", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.24], "Focus": [17741.4], "XYStage": [ 35905.6, -135.3 ] }, "GridColumnIndex": 0 }, { "Label": "Pos23", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.195], "Focus": [17741.5], "XYStage": [ 34990.9, -132.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos24", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.46], "Focus": [17741.4], "XYStage": [ 29678.100000000002, -958.1 ] }, "GridColumnIndex": 0 }, { "Label": "Pos25", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.44], "Focus": [17741.5], "XYStage": [ 28983.600000000002, -939.4000000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos26", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.475], "Focus": [17741.5], "XYStage": [ 23019.7, -122 ] }, "GridColumnIndex": 0 }, { "Label": "Pos27", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.44], "Focus": [17741.7], "XYStage": [ 22428, -116.80000000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos28", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.46], "Focus": [17741.7], "XYStage": [ 16364.900000000001, -181.10000000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos29", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.48000000000002], "Focus": [17741.5], "XYStage": [ 15712.1, -180 ] }, "GridColumnIndex": 0 }, { "Label": "Pos30", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.45000000000002], "Focus": [17741.7], "XYStage": [ 10346.1, 77.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos31", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.49], "Focus": [17741.4], "XYStage": [ 9755.5, 85.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos32", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.475], "Focus": [17741.5], "XYStage": [ 4775.5, 293.90000000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos33", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.445], "Focus": [17741.4], "XYStage": [ 3954.9, 293.90000000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos34", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.455], "Focus": [17741.4], "XYStage": [ -1324.9, 545.6 ] }, "GridColumnIndex": 0 }, { "Label": "Pos35", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.445], "Focus": [17741.3], "XYStage": [ -2097, 576.6 ] }, "GridColumnIndex": 0 } ], "z-step_um": 10, "GridRow": 0, "BitDepth": 16, "Depth": 2, "Channels": 6, "Source": "Micro-Manager", "ChColors": [ -16711681, -256, -65281, -1, -1, -16776961 ], "MetadataVersion": 10, "KeepShutterOpenChannels": true, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536, 65536 ], "CustomIntervals_ms": [], "Height": 1200, "MicroManagerVersion": "1.4.23 20180219", "GridColumn": 0, "UUID": "14a13bf7-c944-4672-b873-fcfbbb7ea13d", "PixelType": "GRAY16", "Width": 1200, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\Yan\\20191218-YC113-S7S8-miseq", "Frames": 1, "Comment": "", "UserName": "Nikon", "PixelSize_um": 1.1, "ROI": [ 0, 0, 1200, 1200 ], "Date": "2019-12-18", "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC", "P-DAPI-high" ], "KeepShutterOpenSlices": true, "IJType": 1, "ComputerName": "ZadorConfocal2", "Positions": 36, "PositionIndex": 9, "CameraTimeout": 20000, "TimeFirst": false }, "640 AO-Name": "AnalogIO", "Vincent-D3-Command": "Close" }, "FrameKey-0-1-5": { "COM4-StopBits": "1", "528 AO-MaxVolts": "5.0000", "XLIGHT Excitation Wheel-State": "1", "470 Shutter-Name": "AnalogIO", "Core-Shutter": "555", "555 AO-Demo": "No", "XLIGHT Dichroic-State": "2", "XYStage-StepSize-Y": "0.1000", "ZPositionUm": 248.82, "XYStage-StepSize-X": "0.1000", "PRIME-FirmwareVersion": "20.28", "PRIME-ClearCycles": "2", "520-Name": "DA Shutter", "405 Shutter-Name": "AnalogIO", "PIEZO-StepSize": "0.0050", "PRIME-ClearMode": "Pre-Exposure", "470 AO-MinVolts": "0.0000", "405 AO-SupportsTriggering": "1", "CameraChannelIndex": 0, "XYStage-StartSpeed": "500.0000", "COM9-StopBits": "2", "405 AO-Description": "NI DAC", "COM4-DelayBetweenCharsMs": "0.0000", "445 AO-Block voltage": "false", "520 AO-TriggerInputLine": "/Dev1/PFI0", "555 AO-Description": "NI DAC", "520 AO-TriggerSampleRate": "100000", "COM4-Verbose": "1", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM4-Name": "COM4", "405 Shutter-Volts": "0.0000", "Vincent-D3-Port": "COM1", "PRIME-PP 4 MIN ADU AFFECTED": "200", "470 Shutter-MinVolts": "0.0000", "PRIME-PP 3 ENABLED": "Yes", "470 AO-Description": "NI DAC", "PRIME-Description": "PVCAM API device adapter", "520 Shutter-MaxVolts": "5.0000", "520 Shutter-Volts": "0.0000", "528 AO-Sequenceable": "1", "PRIME-Binning": "1x1", "555 Shutter-MaxVolts": "5.0000", "528 Shutter-TriggerSequenceLength": "1000", "405 Shutter-Block voltage": "false", "XLIGHT Spin motor-State": "1", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "Vincent-D3-Time to open (ms)": "35.0000", "Vincent-D3-Time to close (ms)": "35.0000", "445 AO-TriggerSampleRate": "100000", "640 Shutter-IOChannel": "Dev1/ao14", "555 AO-Sequenceable": "1", "520 AO-Name": "AnalogIO", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "470 AO-Block voltage": "false", "COM1-Verbose": "1", "COM9-AnswerTimeout": "10000.0000", "528 Shutter-Name": "AnalogIO", "PRIME-Trigger Timeout (secs)": "10", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "NextFrame": 0, "470 AO-TriggerSequenceLength": "1000", "PRIME-TransposeXY": "0", "445 AO-TriggerSequenceLength": "1000", "COM1-Name": "COM1", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "COM9-DataBits": "8", "COM1-AnswerTimeout": "500.0000", "Camera": "", "TE2000-Version": "V2.50-V2.50-V2.10", "528 Shutter-Volts": "0.0000", "XLIGHT Hub-Name": "XLIGHT Hub", "PIEZO-Description": "Ludl stage driver adapter", "PositionName": "Pos9", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSampleRate": "100000", "PRIME-FanSpeedSetpoint": "High", "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "405 AO-TriggerSampleRate": "100000", "470 Shutter-Block voltage": "false", "470-State": "0", "COM3-Parity": "None", "405 AO-Volts": "0.0000", "PRIME-TransposeMirrorY": "0", "PRIME-ChipName": "GS144BSI", "470 AO-Volts": "0.0000", "PRIME-TransposeMirrorX": "0", "445 Shutter-SupportsTriggering": "0", "470 Shutter-SupportsTriggering": "0", "XLIGHT Hub-Description": "XLIGHT hub", "PRIME-PreampOffLimit": "10000", "470 AO-Sequenceable": "1", "528 Shutter-MaxVolts": "5.0000", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "Core-XYStage": "XYStage", "470 AO-TriggerSampleRate": "100000", "PRIME-Timing-PreTriggerDelayNs": "24672000.0000", "528-DA Device": "528 Shutter", "Focus-Description": "Nikon TE2000 focus adapter", "FilterBlock-State": "5", "520 AO-Demo": "No", "COM3-DelayBetweenCharsMs": "0.0000", "470 Shutter-Volts": "0.0000", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "PRIME-PP 2 THRESHOLD": "125", "PRIME-PP 4 THRESHOLD": "75", "XYStage-Acceleration": "4.0000", "470 Shutter-Demo": "No", "PRIME-Name": "PMPCIECam00", "640-DA Device": "640 Shutter", "405 AO-Block voltage": "false", "ElapsedTime-ms": 383590, "PRIME-Offset": "100", "445 AO-IOChannel": "Dev1/ao1", "640 AO-MaxVolts": "5.0000", "PositionIndex": 9, "PRIME-PP 1 ENABLED": "Yes", "COM1-BaudRate": "9600", "640-State": "0", "COM3-Verbose": "1", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "LudlController-TransmissionDelay": "0", "PRIME-FullWellCapacity": "62000", "PRIME-SMARTStreamingEnabled": "No", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "640 Shutter-Description": "NI DAC", "528-Description": "DA device that is used as a shutter", "COM4-Description": "Serial port driver (boost:asio)", "405 Shutter-Description": "NI DAC", "TE2000-Name": "TE2000", "528 Shutter-Description": "NI DAC", "COM9-Description": "Serial port driver (boost:asio)", "LudlController-Name": "LudlController", "445 Shutter-TriggerSequenceLength": "1000", "PRIME-SerialNumber": "A18B203008", "445 AO-Volts": "0.0000", "PRIME-X-dimension": "1200", "PRIME-ShutterCloseDelay": "0", "XLIGHT Disk slider-State": "2", "Position": "Default", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 AO-IOChannel": "Dev1/ao5", "520 AO-IOChannel": "Dev1/ao4", "528 AO-TriggerSequenceLength": "1000", "405 Shutter-MaxVolts": "5.0000", "470-DA Device": "470 Shutter", "PRIME-FTCapable": "No", "Core-ChannelGroup": "Channels", "555 AO-SupportsTriggering": "0", "PRIME-Y-dimension": "1200", "Frame": 0, "555 Shutter-Sequenceable": "1", "PRIME-Timing-ExposureTimeNs": "499998000.0000", "Core-ImageProcessor": "", "528-State": "0", "520 AO-Block voltage": "false", "XLIGHT Excitation Wheel-Label": "QUAD", "640 Shutter-Demo": "No", "COM3-StopBits": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "520 Shutter-IOChannel": "Dev1/ao11", "Width": 1200, "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-SupportsTriggering": "0", "640 AO-TriggerSampleRate": "100000", "520-Description": "DA device that is used as a shutter", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "405 Shutter-Sequenceable": "1", "405 Shutter-SupportsTriggering": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-TriggerSampleRate": "100000", "640 Shutter-MaxVolts": "5.0000", "555 AO-MaxVolts": "5.0000", "520 AO-TriggerSequenceLength": "1000", "COM3-BaudRate": "9600", "COM9-BaudRate": "9600", "Vincent-D3-Channel #": "Ch. #1", "PRIME-PMode": "Normal", "OpticalPath-Label": "Left(100)", "BitDepth": 16, "555 AO-TriggerSequenceLength": "1000", "445 Shutter-Description": "NI DAC", "405 AO-Sequenceable": "1", "PRIME-AcquisitionMethod": "Callbacks", "555 AO-Name": "AnalogIO", "PRIME-ShutterMode": "Pre-Exposure", "COM3-Handshaking": "Off", "445 AO-SupportsTriggering": "0", "ROI": "0-0-1200-1200", "COM9-Verbose": "1", "PRIME-Color": "OFF", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "PRIME-ShutterOpenDelay": "0", "COM1-Parity": "None", "Slice": 5, "SlicePosition": 0, "COM9-DelayBetweenCharsMs": "0.0000", "555-Name": "DA Shutter", "PIEZO-LudlSingleAxisName": "B", "XLIGHT Emission Wheel-Label": "585/11(T)", "640 Shutter-Name": "AnalogIO", "640 AO-Sequenceable": "1", "520 Shutter-Demo": "No", "528 Shutter-Demo": "No", "470-Name": "DA Shutter", "520-DA Device": "520 Shutter", "XYStage-Description": "Ludl XY stage driver adapter", "445 Shutter-MinVolts": "0.0000", "528 AO-TriggerSampleRate": "100000", "405 AO-MinVolts": "0.0000", "405 Shutter-TriggerSequenceLength": "1000", "Height": 1200, "555 AO-Block voltage": "false", "640 Shutter-Volts": "0.0000", "PixelType": "GRAY16", "445 Shutter-Block voltage": "false", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "520 Shutter-TriggerSequenceLength": "1000", "COM9-Name": "COM9", "Core-SLM": "", "555 AO-Volts": "4.5000", "555 Shutter-Block voltage": "false", "XYStage-Speed": "1800.0000", "520 AO-MaxVolts": "5.0000", "528 Shutter-SupportsTriggering": "0", "COM1-Description": "Serial port driver (boost:asio)", "PRIME-MetadataEnabled": "No", "520 Shutter-MinVolts": "0.0000", "470 Shutter-Sequenceable": "1", "PRIME-PreampDelay": "0", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-MinVolts": "0.0000", "ChannelIndex": 1, "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Core-AutoShutter": "1", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "Exposure-ms": 160, "PRIME-PP 2 ENABLED": "Yes", "640 Shutter-TriggerSampleRate": "100000", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "470 Shutter-IOChannel": "Dev1/ao10", "405 AO-Demo": "No", "PixelSizeUm": 1.1, "470 AO-Demo": "No", "470 Shutter-MaxVolts": "5.0000", "Vincent-D3-Name": "Vincent-D3", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FileName": "img_000000000_Il-T_005.tif", "LudlController-Reset": "Operate", "PRIME-Exposure": "160.00", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "445-Name": "DA Shutter", "555 Shutter-Name": "AnalogIO", "520 Shutter-TriggerSampleRate": "100000", "445 Shutter-IOChannel": "Dev1/ao9", "PRIME-TransposeCorrection": "0", "XYStage-StepSize": "0.1000", "445-Description": "DA device that is used as a shutter", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 AO-IOChannel": "Dev1/ao7", "520 Shutter-Name": "AnalogIO", "COM3-DataBits": "8", "640 AO-Volts": "0.0000", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "555 Shutter-IOChannel": "Dev1/ao13", "WaitInterval": null, "520 Shutter-Sequenceable": "1", "YPositionUm": 31506.2, "445-State": "0", "445-DA Device": "445 Shutter", "PRIME-CircularBufferAutoSize": "ON", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "555 AO-TriggerSampleRate": "100000", "445 Shutter-Volts": "0.0000", "XLIGHT Hub-Port": "COM3", "Core-AutoFocus": "", "COM4-Handshaking": "Off", "528 Shutter-MinVolts": "0.0000", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "445 Shutter-Name": "AnalogIO", "528 Shutter-Sequenceable": "1", "XYStage-TransposeMirrorX": "0", "XYStage-TransposeMirrorY": "0", "PRIME-ReadoutRate": "100MHz 16bit", "XYStage-Name": "Stage", "640 AO-SupportsTriggering": "0", "640-Name": "DA Shutter", "640 Shutter-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "640 AO-Block voltage": "false", "445 Shutter-Sequenceable": "1", "470 AO-MaxVolts": "5.0000", "COM4-BaudRate": "9600", "470-Description": "DA device that is used as a shutter", "520 Shutter-Description": "NI DAC", "PRIME-CCDTemperature": "-14.8500", "Time": "2019-12-18 21:36:51 -0500", "640 AO-TriggerSequenceLength": "1000", "470 AO-Name": "AnalogIO", "XLIGHT Disk slider-Label": "Disk Pos 40um", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "Binning": "1x1", "TE2000-ModelType": "1", "COM3-AnswerTimeout": "500.0000", "555 Shutter-SupportsTriggering": "0", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "TE2000-Port": "COM4", "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Description": "NI DAC", "COM4-AnswerTimeout": "500.0000", "COM1-DelayBetweenCharsMs": "0.0000", "FilterBlock-Label": "Confocal", "445 AO-Name": "AnalogIO", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-Timing-ReadoutTimeNs": "24672000.0000", "528 AO-Demo": "No", "520 AO-Sequenceable": "1", "555-Description": "DA device that is used as a shutter", "405-DA Device": "405 Shutter", "405 AO-TriggerSequenceLength": "1000", "640 AO-MinVolts": "0.0000", "640 Shutter-Block voltage": "false", "FrameIndex": 0, "PRIME-Port": "CMOS", "555 AO-MinVolts": "0.0000", "COM3-Description": "Serial port driver (boost:asio)", "528 AO-Description": "NI DAC", "555 AO-IOChannel": "Dev1/ao6", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "555 AO-TriggerInputLine": "/Dev1/PFI0", "PRIME-ActualInterval-ms": "390.5200", "LudlController-Port": "COM9", "445 Shutter-Demo": "No", "PRIME-Trigger-Expose Out-Mux": "1", "SliceIndex": 5, "PRIME-CCDTemperatureSetPoint": "-15.0000", "405 Shutter-MinVolts": "0.0000", "PRIME-ExposeOutMode": "First Row", "AxisPositions": null, "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Volts": "5.0000", "528-Name": "DA Shutter", "555-State": "1", "XLIGHT Dichroic-Label": "QUAD", "445 AO-Sequenceable": "1", "405-Name": "DA Shutter", "405 AO-IOChannel": "Dev1/ao0", "Core-Camera": "PRIME", "Focus-Name": "Focus", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "405 Shutter-IOChannel": "Dev1/ao8", "640 AO-Demo": "No", "640 Shutter-SupportsTriggering": "0", "445 AO-Demo": "No", "555-DA Device": "555 Shutter", "470 Shutter-Description": "NI DAC", "528 AO-Volts": "0.0000", "COM1-DataBits": "8", "Vincent-D3-Address": "x", "520 AO-SupportsTriggering": "0", "405-Description": "DA device that is used as a shutter", "640 Shutter-MinVolts": "0.0000", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "405 AO-Name": "AnalogIO", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "640 AO-TriggerInputLine": "/Dev1/PFI0", "405 AO-TriggerInputLine": "/Dev1/PFI0", "640-Description": "DA device that is used as a shutter", "445 Shutter-TriggerSampleRate": "100000", "470 Shutter-TriggerSequenceLength": "1000", "COM1-StopBits": "1", "520 Shutter-SupportsTriggering": "0", "405-State": "0", "Core-Initialize": "1", "XLIGHT Emission Wheel-State": "3", "520 AO-Description": "NI DAC", "528 AO-TriggerInputLine": "/Dev1/PFI0", "445 Shutter-MaxVolts": "5.0000", "COM9-Handshaking": "Off", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "XLIGHT Spin motor-Label": "On (spinning)", "PRIME-PP 5 ENABLED": "No", "UUID": "8122767c-3f25-44e8-b92f-4cea3d242c2c", "PRIME-TriggerMode": "Internal Trigger", "COM1-Handshaking": "Off", "PRIME-Timing-PostTriggerDelayNs": "49344000.0000", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "Focus-Position": "17741.5000", "528 Shutter-Block voltage": "false", "Channel": "Il-T", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "555 Shutter-Demo": "No", "405 Shutter-Demo": "No", "528 AO-Name": "AnalogIO", "520-State": "0", "Core-Galvo": "", "405 Shutter-TriggerSampleRate": "100000", "Focus-FocusStepSize_(nm)": "100", "PRIME-PixelType": "16bit", "COM4-DataBits": "8", "470 AO-IOChannel": "Dev1/ao2", "520 AO-MinVolts": "0.0000", "COM9-Parity": "None", "PRIME-CircularBufferEnabled": "ON", "PRIME-PP 6 Reset": "No", "640 AO-Description": "NI DAC", "Source": "PRIME", "445 AO-MinVolts": "0.0000", "PIEZO-Name": "Stage", "445 AO-Description": "NI DAC", "PRIME-CircularBufferFrameCount": "46", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "520 AO-Volts": "0.0000", "528 Shutter-TriggerSampleRate": "100000", "XPositionUm": 19731.9, "OpticalPath-State": "4", "PRIME-PP 3 THRESHOLD": "80", "PRIME-PVCAM Version": "3.7.1", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Gain": "1-HDR", "640 Shutter-TriggerSequenceLength": "1000", "528 AO-SupportsTriggering": "0", "445 AO-TriggerInputLine": "/Dev1/PFI0", "OpticalPath-Name": "OpticalPath", "PRIME-PP 4 ENABLED": "Yes", "PRIME-PVCAM Adapter Version": "1.3.29", "COM3-Name": "COM3", "405 AO-MaxVolts": "5.0000", "520 Shutter-Block voltage": "false", "PRIME-Timing-ClearingTimeNs": "49344000.0000", "Core-Focus": "PIEZO", "528 AO-Block voltage": "false", "TE2000-Description": "Nikon TE2000 hub", "528 AO-MinVolts": "0.0000", "COM4-Parity": "None", "Summary": { "Slices": 11, "ChContrastMin": [ 0, 0, 0, 0, 0, 0 ], "PixelAspect": 1, "SlicesFirst": true, "Interval_ms": 0, "Time": "2019-12-18 21:36:42 -0500", "Prefix": "seq_4", "InitialPositionList": [ { "Label": "Pos0", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.21], "Focus": [17741.5], "XYStage": [ 44542.200000000004, 31636.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos1", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.22], "Focus": [17741.4], "XYStage": [ 43801.100000000006, 31637.9 ] }, "GridColumnIndex": 0 }, { "Label": "Pos2", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.5], "XYStage": [ 38561.3, 32040.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos3", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.185], "Focus": [17741.3], "XYStage": [ 37698.4, 32041.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos4", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.16], "Focus": [17741.5], "XYStage": [ 32375.4, 31758.9 ] }, "GridColumnIndex": 0 }, { "Label": "Pos5", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.17000000000002], "Focus": [17741.3], "XYStage": [ 31770.7, 31758.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos6", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.4], "XYStage": [ 26536.800000000003, 31068.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos7", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.205], "Focus": [17741.4], "XYStage": [ 25420.2, 31068.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos8", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.85], "Focus": [17741.3], "XYStage": [ 21059, 31508 ] }, "GridColumnIndex": 0 }, { "Label": "Pos9", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.82], "Focus": [17741.3], "XYStage": [ 19731.9, 31506.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos10", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.79], "Focus": [17741.4], "XYStage": [ 14528.5, 32007.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos11", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.83], "Focus": [17741.2], "XYStage": [ 13830.300000000001, 32009.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos12", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.79], "Focus": [17741.3], "XYStage": [ 8775.300000000001, 32017.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos13", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.815], "Focus": [17741.3], "XYStage": [ 8109.200000000001, 32008.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos14", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.805], "Focus": [17741.3], "XYStage": [ 3483.3, 31908.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos15", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.82], "Focus": [17741.2], "XYStage": [ 2686.8, 31903.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos16", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [211.48000000000002], "Focus": [17741.1], "XYStage": [ -1978.9, 31973.600000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos17", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [211.51500000000001], "Focus": [17741.1], "XYStage": [ -2957.8, 31982.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos18", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.195], "Focus": [17741.5], "XYStage": [ 47747.4, -576.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos19", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.23000000000002], "Focus": [17741.6], "XYStage": [ 47053.9, -576.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos20", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.205], "Focus": [17741.4], "XYStage": [ 41871.200000000004, -479.8 ] }, "GridColumnIndex": 0 }, { "Label": "Pos21", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.4], "XYStage": [ 40882.600000000006, -392 ] }, "GridColumnIndex": 0 }, { "Label": "Pos22", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.24], "Focus": [17741.4], "XYStage": [ 35905.6, -135.3 ] }, "GridColumnIndex": 0 }, { "Label": "Pos23", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.195], "Focus": [17741.5], "XYStage": [ 34990.9, -132.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos24", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.46], "Focus": [17741.4], "XYStage": [ 29678.100000000002, -958.1 ] }, "GridColumnIndex": 0 }, { "Label": "Pos25", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.44], "Focus": [17741.5], "XYStage": [ 28983.600000000002, -939.4000000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos26", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.475], "Focus": [17741.5], "XYStage": [ 23019.7, -122 ] }, "GridColumnIndex": 0 }, { "Label": "Pos27", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.44], "Focus": [17741.7], "XYStage": [ 22428, -116.80000000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos28", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.46], "Focus": [17741.7], "XYStage": [ 16364.900000000001, -181.10000000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos29", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.48000000000002], "Focus": [17741.5], "XYStage": [ 15712.1, -180 ] }, "GridColumnIndex": 0 }, { "Label": "Pos30", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.45000000000002], "Focus": [17741.7], "XYStage": [ 10346.1, 77.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos31", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.49], "Focus": [17741.4], "XYStage": [ 9755.5, 85.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos32", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.475], "Focus": [17741.5], "XYStage": [ 4775.5, 293.90000000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos33", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.445], "Focus": [17741.4], "XYStage": [ 3954.9, 293.90000000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos34", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.455], "Focus": [17741.4], "XYStage": [ -1324.9, 545.6 ] }, "GridColumnIndex": 0 }, { "Label": "Pos35", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.445], "Focus": [17741.3], "XYStage": [ -2097, 576.6 ] }, "GridColumnIndex": 0 } ], "z-step_um": 10, "GridRow": 0, "BitDepth": 16, "Depth": 2, "Channels": 6, "Source": "Micro-Manager", "ChColors": [ -16711681, -256, -65281, -1, -1, -16776961 ], "MetadataVersion": 10, "KeepShutterOpenChannels": true, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536, 65536 ], "CustomIntervals_ms": [], "Height": 1200, "MicroManagerVersion": "1.4.23 20180219", "GridColumn": 0, "UUID": "14a13bf7-c944-4672-b873-fcfbbb7ea13d", "PixelType": "GRAY16", "Width": 1200, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\Yan\\20191218-YC113-S7S8-miseq", "Frames": 1, "Comment": "", "UserName": "Nikon", "PixelSize_um": 1.1, "ROI": [ 0, 0, 1200, 1200 ], "Date": "2019-12-18", "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC", "P-DAPI-high" ], "KeepShutterOpenSlices": true, "IJType": 1, "ComputerName": "ZadorConfocal2", "Positions": 36, "PositionIndex": 9, "CameraTimeout": 20000, "TimeFirst": false }, "640 AO-Name": "AnalogIO", "Vincent-D3-Command": "Close" }, "FrameKey-0-1-6": { "COM4-StopBits": "1", "528 AO-MaxVolts": "5.0000", "XLIGHT Excitation Wheel-State": "1", "470 Shutter-Name": "AnalogIO", "Core-Shutter": "555", "555 AO-Demo": "No", "XLIGHT Dichroic-State": "2", "XYStage-StepSize-Y": "0.1000", "ZPositionUm": 258.82, "XYStage-StepSize-X": "0.1000", "PRIME-FirmwareVersion": "20.28", "PRIME-ClearCycles": "2", "520-Name": "DA Shutter", "405 Shutter-Name": "AnalogIO", "PIEZO-StepSize": "0.0050", "PRIME-ClearMode": "Pre-Exposure", "470 AO-MinVolts": "0.0000", "405 AO-SupportsTriggering": "1", "CameraChannelIndex": 0, "XYStage-StartSpeed": "500.0000", "COM9-StopBits": "2", "405 AO-Description": "NI DAC", "COM4-DelayBetweenCharsMs": "0.0000", "445 AO-Block voltage": "false", "520 AO-TriggerInputLine": "/Dev1/PFI0", "555 AO-Description": "NI DAC", "520 AO-TriggerSampleRate": "100000", "COM4-Verbose": "1", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM4-Name": "COM4", "405 Shutter-Volts": "0.0000", "Vincent-D3-Port": "COM1", "PRIME-PP 4 MIN ADU AFFECTED": "200", "470 Shutter-MinVolts": "0.0000", "PRIME-PP 3 ENABLED": "Yes", "470 AO-Description": "NI DAC", "PRIME-Description": "PVCAM API device adapter", "520 Shutter-MaxVolts": "5.0000", "520 Shutter-Volts": "0.0000", "528 AO-Sequenceable": "1", "PRIME-Binning": "1x1", "555 Shutter-MaxVolts": "5.0000", "528 Shutter-TriggerSequenceLength": "1000", "405 Shutter-Block voltage": "false", "XLIGHT Spin motor-State": "1", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "Vincent-D3-Time to open (ms)": "35.0000", "Vincent-D3-Time to close (ms)": "35.0000", "445 AO-TriggerSampleRate": "100000", "640 Shutter-IOChannel": "Dev1/ao14", "555 AO-Sequenceable": "1", "520 AO-Name": "AnalogIO", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "470 AO-Block voltage": "false", "COM1-Verbose": "1", "COM9-AnswerTimeout": "10000.0000", "528 Shutter-Name": "AnalogIO", "PRIME-Trigger Timeout (secs)": "10", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "NextFrame": 0, "470 AO-TriggerSequenceLength": "1000", "PRIME-TransposeXY": "0", "445 AO-TriggerSequenceLength": "1000", "COM1-Name": "COM1", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "COM9-DataBits": "8", "COM1-AnswerTimeout": "500.0000", "Camera": "", "TE2000-Version": "V2.50-V2.50-V2.10", "528 Shutter-Volts": "0.0000", "XLIGHT Hub-Name": "XLIGHT Hub", "PIEZO-Description": "Ludl stage driver adapter", "PositionName": "Pos9", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSampleRate": "100000", "PRIME-FanSpeedSetpoint": "High", "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "405 AO-TriggerSampleRate": "100000", "470 Shutter-Block voltage": "false", "470-State": "0", "COM3-Parity": "None", "405 AO-Volts": "0.0000", "PRIME-TransposeMirrorY": "0", "PRIME-ChipName": "GS144BSI", "470 AO-Volts": "0.0000", "PRIME-TransposeMirrorX": "0", "445 Shutter-SupportsTriggering": "0", "470 Shutter-SupportsTriggering": "0", "XLIGHT Hub-Description": "XLIGHT hub", "PRIME-PreampOffLimit": "10000", "470 AO-Sequenceable": "1", "528 Shutter-MaxVolts": "5.0000", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "Core-XYStage": "XYStage", "470 AO-TriggerSampleRate": "100000", "PRIME-Timing-PreTriggerDelayNs": "24672000.0000", "528-DA Device": "528 Shutter", "Focus-Description": "Nikon TE2000 focus adapter", "FilterBlock-State": "5", "520 AO-Demo": "No", "COM3-DelayBetweenCharsMs": "0.0000", "470 Shutter-Volts": "0.0000", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "PRIME-PP 2 THRESHOLD": "125", "PRIME-PP 4 THRESHOLD": "75", "XYStage-Acceleration": "4.0000", "470 Shutter-Demo": "No", "PRIME-Name": "PMPCIECam00", "640-DA Device": "640 Shutter", "405 AO-Block voltage": "false", "ElapsedTime-ms": 384042, "PRIME-Offset": "100", "445 AO-IOChannel": "Dev1/ao1", "640 AO-MaxVolts": "5.0000", "PositionIndex": 9, "PRIME-PP 1 ENABLED": "Yes", "COM1-BaudRate": "9600", "640-State": "0", "COM3-Verbose": "1", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "LudlController-TransmissionDelay": "0", "PRIME-FullWellCapacity": "62000", "PRIME-SMARTStreamingEnabled": "No", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "640 Shutter-Description": "NI DAC", "528-Description": "DA device that is used as a shutter", "COM4-Description": "Serial port driver (boost:asio)", "405 Shutter-Description": "NI DAC", "TE2000-Name": "TE2000", "528 Shutter-Description": "NI DAC", "COM9-Description": "Serial port driver (boost:asio)", "LudlController-Name": "LudlController", "445 Shutter-TriggerSequenceLength": "1000", "PRIME-SerialNumber": "A18B203008", "445 AO-Volts": "0.0000", "PRIME-X-dimension": "1200", "PRIME-ShutterCloseDelay": "0", "XLIGHT Disk slider-State": "2", "Position": "Default", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 AO-IOChannel": "Dev1/ao5", "520 AO-IOChannel": "Dev1/ao4", "528 AO-TriggerSequenceLength": "1000", "405 Shutter-MaxVolts": "5.0000", "470-DA Device": "470 Shutter", "PRIME-FTCapable": "No", "Core-ChannelGroup": "Channels", "555 AO-SupportsTriggering": "0", "PRIME-Y-dimension": "1200", "Frame": 0, "555 Shutter-Sequenceable": "1", "PRIME-Timing-ExposureTimeNs": "499998000.0000", "Core-ImageProcessor": "", "528-State": "0", "520 AO-Block voltage": "false", "XLIGHT Excitation Wheel-Label": "QUAD", "640 Shutter-Demo": "No", "COM3-StopBits": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "520 Shutter-IOChannel": "Dev1/ao11", "Width": 1200, "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-SupportsTriggering": "0", "640 AO-TriggerSampleRate": "100000", "520-Description": "DA device that is used as a shutter", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "405 Shutter-Sequenceable": "1", "405 Shutter-SupportsTriggering": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-TriggerSampleRate": "100000", "640 Shutter-MaxVolts": "5.0000", "555 AO-MaxVolts": "5.0000", "520 AO-TriggerSequenceLength": "1000", "COM3-BaudRate": "9600", "COM9-BaudRate": "9600", "Vincent-D3-Channel #": "Ch. #1", "PRIME-PMode": "Normal", "OpticalPath-Label": "Left(100)", "BitDepth": 16, "555 AO-TriggerSequenceLength": "1000", "445 Shutter-Description": "NI DAC", "405 AO-Sequenceable": "1", "PRIME-AcquisitionMethod": "Callbacks", "555 AO-Name": "AnalogIO", "PRIME-ShutterMode": "Pre-Exposure", "COM3-Handshaking": "Off", "445 AO-SupportsTriggering": "0", "ROI": "0-0-1200-1200", "COM9-Verbose": "1", "PRIME-Color": "OFF", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "PRIME-ShutterOpenDelay": "0", "COM1-Parity": "None", "Slice": 6, "SlicePosition": 10, "COM9-DelayBetweenCharsMs": "0.0000", "555-Name": "DA Shutter", "PIEZO-LudlSingleAxisName": "B", "XLIGHT Emission Wheel-Label": "585/11(T)", "640 Shutter-Name": "AnalogIO", "640 AO-Sequenceable": "1", "520 Shutter-Demo": "No", "528 Shutter-Demo": "No", "470-Name": "DA Shutter", "520-DA Device": "520 Shutter", "XYStage-Description": "Ludl XY stage driver adapter", "445 Shutter-MinVolts": "0.0000", "528 AO-TriggerSampleRate": "100000", "405 AO-MinVolts": "0.0000", "405 Shutter-TriggerSequenceLength": "1000", "Height": 1200, "555 AO-Block voltage": "false", "640 Shutter-Volts": "0.0000", "PixelType": "GRAY16", "445 Shutter-Block voltage": "false", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "520 Shutter-TriggerSequenceLength": "1000", "COM9-Name": "COM9", "Core-SLM": "", "555 AO-Volts": "4.5000", "555 Shutter-Block voltage": "false", "XYStage-Speed": "1800.0000", "520 AO-MaxVolts": "5.0000", "528 Shutter-SupportsTriggering": "0", "COM1-Description": "Serial port driver (boost:asio)", "PRIME-MetadataEnabled": "No", "520 Shutter-MinVolts": "0.0000", "470 Shutter-Sequenceable": "1", "PRIME-PreampDelay": "0", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-MinVolts": "0.0000", "ChannelIndex": 1, "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Core-AutoShutter": "1", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "Exposure-ms": 160, "PRIME-PP 2 ENABLED": "Yes", "640 Shutter-TriggerSampleRate": "100000", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "470 Shutter-IOChannel": "Dev1/ao10", "405 AO-Demo": "No", "PixelSizeUm": 1.1, "470 AO-Demo": "No", "470 Shutter-MaxVolts": "5.0000", "Vincent-D3-Name": "Vincent-D3", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FileName": "img_000000000_Il-T_006.tif", "LudlController-Reset": "Operate", "PRIME-Exposure": "160.00", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "445-Name": "DA Shutter", "555 Shutter-Name": "AnalogIO", "520 Shutter-TriggerSampleRate": "100000", "445 Shutter-IOChannel": "Dev1/ao9", "PRIME-TransposeCorrection": "0", "XYStage-StepSize": "0.1000", "445-Description": "DA device that is used as a shutter", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 AO-IOChannel": "Dev1/ao7", "520 Shutter-Name": "AnalogIO", "COM3-DataBits": "8", "640 AO-Volts": "0.0000", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "555 Shutter-IOChannel": "Dev1/ao13", "WaitInterval": null, "520 Shutter-Sequenceable": "1", "YPositionUm": 31506.2, "445-State": "0", "445-DA Device": "445 Shutter", "PRIME-CircularBufferAutoSize": "ON", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "555 AO-TriggerSampleRate": "100000", "445 Shutter-Volts": "0.0000", "XLIGHT Hub-Port": "COM3", "Core-AutoFocus": "", "COM4-Handshaking": "Off", "528 Shutter-MinVolts": "0.0000", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "445 Shutter-Name": "AnalogIO", "528 Shutter-Sequenceable": "1", "XYStage-TransposeMirrorX": "0", "XYStage-TransposeMirrorY": "0", "PRIME-ReadoutRate": "100MHz 16bit", "XYStage-Name": "Stage", "640 AO-SupportsTriggering": "0", "640-Name": "DA Shutter", "640 Shutter-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "640 AO-Block voltage": "false", "445 Shutter-Sequenceable": "1", "470 AO-MaxVolts": "5.0000", "COM4-BaudRate": "9600", "470-Description": "DA device that is used as a shutter", "520 Shutter-Description": "NI DAC", "PRIME-CCDTemperature": "-14.8500", "Time": "2019-12-18 21:36:51 -0500", "640 AO-TriggerSequenceLength": "1000", "470 AO-Name": "AnalogIO", "XLIGHT Disk slider-Label": "Disk Pos 40um", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "Binning": "1x1", "TE2000-ModelType": "1", "COM3-AnswerTimeout": "500.0000", "555 Shutter-SupportsTriggering": "0", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "TE2000-Port": "COM4", "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Description": "NI DAC", "COM4-AnswerTimeout": "500.0000", "COM1-DelayBetweenCharsMs": "0.0000", "FilterBlock-Label": "Confocal", "445 AO-Name": "AnalogIO", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-Timing-ReadoutTimeNs": "24672000.0000", "528 AO-Demo": "No", "520 AO-Sequenceable": "1", "555-Description": "DA device that is used as a shutter", "405-DA Device": "405 Shutter", "405 AO-TriggerSequenceLength": "1000", "640 AO-MinVolts": "0.0000", "640 Shutter-Block voltage": "false", "FrameIndex": 0, "PRIME-Port": "CMOS", "555 AO-MinVolts": "0.0000", "COM3-Description": "Serial port driver (boost:asio)", "528 AO-Description": "NI DAC", "555 AO-IOChannel": "Dev1/ao6", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "555 AO-TriggerInputLine": "/Dev1/PFI0", "PRIME-ActualInterval-ms": "390.5200", "LudlController-Port": "COM9", "445 Shutter-Demo": "No", "PRIME-Trigger-Expose Out-Mux": "1", "SliceIndex": 6, "PRIME-CCDTemperatureSetPoint": "-15.0000", "405 Shutter-MinVolts": "0.0000", "PRIME-ExposeOutMode": "First Row", "AxisPositions": null, "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Volts": "5.0000", "528-Name": "DA Shutter", "555-State": "1", "XLIGHT Dichroic-Label": "QUAD", "445 AO-Sequenceable": "1", "405-Name": "DA Shutter", "405 AO-IOChannel": "Dev1/ao0", "Core-Camera": "PRIME", "Focus-Name": "Focus", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "405 Shutter-IOChannel": "Dev1/ao8", "640 AO-Demo": "No", "640 Shutter-SupportsTriggering": "0", "445 AO-Demo": "No", "555-DA Device": "555 Shutter", "470 Shutter-Description": "NI DAC", "528 AO-Volts": "0.0000", "COM1-DataBits": "8", "Vincent-D3-Address": "x", "520 AO-SupportsTriggering": "0", "405-Description": "DA device that is used as a shutter", "640 Shutter-MinVolts": "0.0000", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "405 AO-Name": "AnalogIO", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "640 AO-TriggerInputLine": "/Dev1/PFI0", "405 AO-TriggerInputLine": "/Dev1/PFI0", "640-Description": "DA device that is used as a shutter", "445 Shutter-TriggerSampleRate": "100000", "470 Shutter-TriggerSequenceLength": "1000", "COM1-StopBits": "1", "520 Shutter-SupportsTriggering": "0", "405-State": "0", "Core-Initialize": "1", "XLIGHT Emission Wheel-State": "3", "520 AO-Description": "NI DAC", "528 AO-TriggerInputLine": "/Dev1/PFI0", "445 Shutter-MaxVolts": "5.0000", "COM9-Handshaking": "Off", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "XLIGHT Spin motor-Label": "On (spinning)", "PRIME-PP 5 ENABLED": "No", "UUID": "35e53d93-0b6a-4f3c-9e36-424502fd5a4c", "PRIME-TriggerMode": "Internal Trigger", "COM1-Handshaking": "Off", "PRIME-Timing-PostTriggerDelayNs": "49344000.0000", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "Focus-Position": "17741.5000", "528 Shutter-Block voltage": "false", "Channel": "Il-T", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "555 Shutter-Demo": "No", "405 Shutter-Demo": "No", "528 AO-Name": "AnalogIO", "520-State": "0", "Core-Galvo": "", "405 Shutter-TriggerSampleRate": "100000", "Focus-FocusStepSize_(nm)": "100", "PRIME-PixelType": "16bit", "COM4-DataBits": "8", "470 AO-IOChannel": "Dev1/ao2", "520 AO-MinVolts": "0.0000", "COM9-Parity": "None", "PRIME-CircularBufferEnabled": "ON", "PRIME-PP 6 Reset": "No", "640 AO-Description": "NI DAC", "Source": "PRIME", "445 AO-MinVolts": "0.0000", "PIEZO-Name": "Stage", "445 AO-Description": "NI DAC", "PRIME-CircularBufferFrameCount": "46", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "520 AO-Volts": "0.0000", "528 Shutter-TriggerSampleRate": "100000", "XPositionUm": 19731.9, "OpticalPath-State": "4", "PRIME-PP 3 THRESHOLD": "80", "PRIME-PVCAM Version": "3.7.1", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Gain": "1-HDR", "640 Shutter-TriggerSequenceLength": "1000", "528 AO-SupportsTriggering": "0", "445 AO-TriggerInputLine": "/Dev1/PFI0", "OpticalPath-Name": "OpticalPath", "PRIME-PP 4 ENABLED": "Yes", "PRIME-PVCAM Adapter Version": "1.3.29", "COM3-Name": "COM3", "405 AO-MaxVolts": "5.0000", "520 Shutter-Block voltage": "false", "PRIME-Timing-ClearingTimeNs": "49344000.0000", "Core-Focus": "PIEZO", "528 AO-Block voltage": "false", "TE2000-Description": "Nikon TE2000 hub", "528 AO-MinVolts": "0.0000", "COM4-Parity": "None", "Summary": { "Slices": 11, "ChContrastMin": [ 0, 0, 0, 0, 0, 0 ], "PixelAspect": 1, "SlicesFirst": true, "Interval_ms": 0, "Time": "2019-12-18 21:36:42 -0500", "Prefix": "seq_4", "InitialPositionList": [ { "Label": "Pos0", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.21], "Focus": [17741.5], "XYStage": [ 44542.200000000004, 31636.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos1", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.22], "Focus": [17741.4], "XYStage": [ 43801.100000000006, 31637.9 ] }, "GridColumnIndex": 0 }, { "Label": "Pos2", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.5], "XYStage": [ 38561.3, 32040.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos3", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.185], "Focus": [17741.3], "XYStage": [ 37698.4, 32041.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos4", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.16], "Focus": [17741.5], "XYStage": [ 32375.4, 31758.9 ] }, "GridColumnIndex": 0 }, { "Label": "Pos5", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.17000000000002], "Focus": [17741.3], "XYStage": [ 31770.7, 31758.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos6", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.4], "XYStage": [ 26536.800000000003, 31068.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos7", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.205], "Focus": [17741.4], "XYStage": [ 25420.2, 31068.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos8", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.85], "Focus": [17741.3], "XYStage": [ 21059, 31508 ] }, "GridColumnIndex": 0 }, { "Label": "Pos9", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.82], "Focus": [17741.3], "XYStage": [ 19731.9, 31506.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos10", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.79], "Focus": [17741.4], "XYStage": [ 14528.5, 32007.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos11", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.83], "Focus": [17741.2], "XYStage": [ 13830.300000000001, 32009.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos12", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.79], "Focus": [17741.3], "XYStage": [ 8775.300000000001, 32017.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos13", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.815], "Focus": [17741.3], "XYStage": [ 8109.200000000001, 32008.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos14", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.805], "Focus": [17741.3], "XYStage": [ 3483.3, 31908.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos15", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.82], "Focus": [17741.2], "XYStage": [ 2686.8, 31903.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos16", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [211.48000000000002], "Focus": [17741.1], "XYStage": [ -1978.9, 31973.600000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos17", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [211.51500000000001], "Focus": [17741.1], "XYStage": [ -2957.8, 31982.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos18", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.195], "Focus": [17741.5], "XYStage": [ 47747.4, -576.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos19", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.23000000000002], "Focus": [17741.6], "XYStage": [ 47053.9, -576.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos20", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.205], "Focus": [17741.4], "XYStage": [ 41871.200000000004, -479.8 ] }, "GridColumnIndex": 0 }, { "Label": "Pos21", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.4], "XYStage": [ 40882.600000000006, -392 ] }, "GridColumnIndex": 0 }, { "Label": "Pos22", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.24], "Focus": [17741.4], "XYStage": [ 35905.6, -135.3 ] }, "GridColumnIndex": 0 }, { "Label": "Pos23", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.195], "Focus": [17741.5], "XYStage": [ 34990.9, -132.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos24", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.46], "Focus": [17741.4], "XYStage": [ 29678.100000000002, -958.1 ] }, "GridColumnIndex": 0 }, { "Label": "Pos25", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.44], "Focus": [17741.5], "XYStage": [ 28983.600000000002, -939.4000000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos26", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.475], "Focus": [17741.5], "XYStage": [ 23019.7, -122 ] }, "GridColumnIndex": 0 }, { "Label": "Pos27", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.44], "Focus": [17741.7], "XYStage": [ 22428, -116.80000000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos28", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.46], "Focus": [17741.7], "XYStage": [ 16364.900000000001, -181.10000000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos29", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.48000000000002], "Focus": [17741.5], "XYStage": [ 15712.1, -180 ] }, "GridColumnIndex": 0 }, { "Label": "Pos30", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.45000000000002], "Focus": [17741.7], "XYStage": [ 10346.1, 77.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos31", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.49], "Focus": [17741.4], "XYStage": [ 9755.5, 85.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos32", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.475], "Focus": [17741.5], "XYStage": [ 4775.5, 293.90000000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos33", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.445], "Focus": [17741.4], "XYStage": [ 3954.9, 293.90000000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos34", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.455], "Focus": [17741.4], "XYStage": [ -1324.9, 545.6 ] }, "GridColumnIndex": 0 }, { "Label": "Pos35", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.445], "Focus": [17741.3], "XYStage": [ -2097, 576.6 ] }, "GridColumnIndex": 0 } ], "z-step_um": 10, "GridRow": 0, "BitDepth": 16, "Depth": 2, "Channels": 6, "Source": "Micro-Manager", "ChColors": [ -16711681, -256, -65281, -1, -1, -16776961 ], "MetadataVersion": 10, "KeepShutterOpenChannels": true, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536, 65536 ], "CustomIntervals_ms": [], "Height": 1200, "MicroManagerVersion": "1.4.23 20180219", "GridColumn": 0, "UUID": "14a13bf7-c944-4672-b873-fcfbbb7ea13d", "PixelType": "GRAY16", "Width": 1200, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\Yan\\20191218-YC113-S7S8-miseq", "Frames": 1, "Comment": "", "UserName": "Nikon", "PixelSize_um": 1.1, "ROI": [ 0, 0, 1200, 1200 ], "Date": "2019-12-18", "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC", "P-DAPI-high" ], "KeepShutterOpenSlices": true, "IJType": 1, "ComputerName": "ZadorConfocal2", "Positions": 36, "PositionIndex": 9, "CameraTimeout": 20000, "TimeFirst": false }, "640 AO-Name": "AnalogIO", "Vincent-D3-Command": "Close" }, "FrameKey-0-1-7": { "COM4-StopBits": "1", "528 AO-MaxVolts": "5.0000", "XLIGHT Excitation Wheel-State": "1", "470 Shutter-Name": "AnalogIO", "Core-Shutter": "555", "555 AO-Demo": "No", "XLIGHT Dichroic-State": "2", "XYStage-StepSize-Y": "0.1000", "ZPositionUm": 268.82, "XYStage-StepSize-X": "0.1000", "PRIME-FirmwareVersion": "20.28", "PRIME-ClearCycles": "2", "520-Name": "DA Shutter", "405 Shutter-Name": "AnalogIO", "PIEZO-StepSize": "0.0050", "PRIME-ClearMode": "Pre-Exposure", "470 AO-MinVolts": "0.0000", "405 AO-SupportsTriggering": "1", "CameraChannelIndex": 0, "XYStage-StartSpeed": "500.0000", "COM9-StopBits": "2", "405 AO-Description": "NI DAC", "COM4-DelayBetweenCharsMs": "0.0000", "445 AO-Block voltage": "false", "520 AO-TriggerInputLine": "/Dev1/PFI0", "555 AO-Description": "NI DAC", "520 AO-TriggerSampleRate": "100000", "COM4-Verbose": "1", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM4-Name": "COM4", "405 Shutter-Volts": "0.0000", "Vincent-D3-Port": "COM1", "PRIME-PP 4 MIN ADU AFFECTED": "200", "470 Shutter-MinVolts": "0.0000", "PRIME-PP 3 ENABLED": "Yes", "470 AO-Description": "NI DAC", "PRIME-Description": "PVCAM API device adapter", "520 Shutter-MaxVolts": "5.0000", "520 Shutter-Volts": "0.0000", "528 AO-Sequenceable": "1", "PRIME-Binning": "1x1", "555 Shutter-MaxVolts": "5.0000", "528 Shutter-TriggerSequenceLength": "1000", "405 Shutter-Block voltage": "false", "XLIGHT Spin motor-State": "1", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "Vincent-D3-Time to open (ms)": "35.0000", "Vincent-D3-Time to close (ms)": "35.0000", "445 AO-TriggerSampleRate": "100000", "640 Shutter-IOChannel": "Dev1/ao14", "555 AO-Sequenceable": "1", "520 AO-Name": "AnalogIO", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "470 AO-Block voltage": "false", "COM1-Verbose": "1", "COM9-AnswerTimeout": "10000.0000", "528 Shutter-Name": "AnalogIO", "PRIME-Trigger Timeout (secs)": "10", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "NextFrame": 0, "470 AO-TriggerSequenceLength": "1000", "PRIME-TransposeXY": "0", "445 AO-TriggerSequenceLength": "1000", "COM1-Name": "COM1", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "COM9-DataBits": "8", "COM1-AnswerTimeout": "500.0000", "Camera": "", "TE2000-Version": "V2.50-V2.50-V2.10", "528 Shutter-Volts": "0.0000", "XLIGHT Hub-Name": "XLIGHT Hub", "PIEZO-Description": "Ludl stage driver adapter", "PositionName": "Pos9", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSampleRate": "100000", "PRIME-FanSpeedSetpoint": "High", "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "405 AO-TriggerSampleRate": "100000", "470 Shutter-Block voltage": "false", "470-State": "0", "COM3-Parity": "None", "405 AO-Volts": "0.0000", "PRIME-TransposeMirrorY": "0", "PRIME-ChipName": "GS144BSI", "470 AO-Volts": "0.0000", "PRIME-TransposeMirrorX": "0", "445 Shutter-SupportsTriggering": "0", "470 Shutter-SupportsTriggering": "0", "XLIGHT Hub-Description": "XLIGHT hub", "PRIME-PreampOffLimit": "10000", "470 AO-Sequenceable": "1", "528 Shutter-MaxVolts": "5.0000", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "Core-XYStage": "XYStage", "470 AO-TriggerSampleRate": "100000", "PRIME-Timing-PreTriggerDelayNs": "24672000.0000", "528-DA Device": "528 Shutter", "Focus-Description": "Nikon TE2000 focus adapter", "FilterBlock-State": "5", "520 AO-Demo": "No", "COM3-DelayBetweenCharsMs": "0.0000", "470 Shutter-Volts": "0.0000", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "PRIME-PP 2 THRESHOLD": "125", "PRIME-PP 4 THRESHOLD": "75", "XYStage-Acceleration": "4.0000", "470 Shutter-Demo": "No", "PRIME-Name": "PMPCIECam00", "640-DA Device": "640 Shutter", "405 AO-Block voltage": "false", "ElapsedTime-ms": 384442, "PRIME-Offset": "100", "445 AO-IOChannel": "Dev1/ao1", "640 AO-MaxVolts": "5.0000", "PositionIndex": 9, "PRIME-PP 1 ENABLED": "Yes", "COM1-BaudRate": "9600", "640-State": "0", "COM3-Verbose": "1", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "LudlController-TransmissionDelay": "0", "PRIME-FullWellCapacity": "62000", "PRIME-SMARTStreamingEnabled": "No", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "640 Shutter-Description": "NI DAC", "528-Description": "DA device that is used as a shutter", "COM4-Description": "Serial port driver (boost:asio)", "405 Shutter-Description": "NI DAC", "TE2000-Name": "TE2000", "528 Shutter-Description": "NI DAC", "COM9-Description": "Serial port driver (boost:asio)", "LudlController-Name": "LudlController", "445 Shutter-TriggerSequenceLength": "1000", "PRIME-SerialNumber": "A18B203008", "445 AO-Volts": "0.0000", "PRIME-X-dimension": "1200", "PRIME-ShutterCloseDelay": "0", "XLIGHT Disk slider-State": "2", "Position": "Default", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 AO-IOChannel": "Dev1/ao5", "520 AO-IOChannel": "Dev1/ao4", "528 AO-TriggerSequenceLength": "1000", "405 Shutter-MaxVolts": "5.0000", "470-DA Device": "470 Shutter", "PRIME-FTCapable": "No", "Core-ChannelGroup": "Channels", "555 AO-SupportsTriggering": "0", "PRIME-Y-dimension": "1200", "Frame": 0, "555 Shutter-Sequenceable": "1", "PRIME-Timing-ExposureTimeNs": "499998000.0000", "Core-ImageProcessor": "", "528-State": "0", "520 AO-Block voltage": "false", "XLIGHT Excitation Wheel-Label": "QUAD", "640 Shutter-Demo": "No", "COM3-StopBits": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "520 Shutter-IOChannel": "Dev1/ao11", "Width": 1200, "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-SupportsTriggering": "0", "640 AO-TriggerSampleRate": "100000", "520-Description": "DA device that is used as a shutter", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "405 Shutter-Sequenceable": "1", "405 Shutter-SupportsTriggering": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-TriggerSampleRate": "100000", "640 Shutter-MaxVolts": "5.0000", "555 AO-MaxVolts": "5.0000", "520 AO-TriggerSequenceLength": "1000", "COM3-BaudRate": "9600", "COM9-BaudRate": "9600", "Vincent-D3-Channel #": "Ch. #1", "PRIME-PMode": "Normal", "OpticalPath-Label": "Left(100)", "BitDepth": 16, "555 AO-TriggerSequenceLength": "1000", "445 Shutter-Description": "NI DAC", "405 AO-Sequenceable": "1", "PRIME-AcquisitionMethod": "Callbacks", "555 AO-Name": "AnalogIO", "PRIME-ShutterMode": "Pre-Exposure", "COM3-Handshaking": "Off", "445 AO-SupportsTriggering": "0", "ROI": "0-0-1200-1200", "COM9-Verbose": "1", "PRIME-Color": "OFF", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "PRIME-ShutterOpenDelay": "0", "COM1-Parity": "None", "Slice": 7, "SlicePosition": 20, "COM9-DelayBetweenCharsMs": "0.0000", "555-Name": "DA Shutter", "PIEZO-LudlSingleAxisName": "B", "XLIGHT Emission Wheel-Label": "585/11(T)", "640 Shutter-Name": "AnalogIO", "640 AO-Sequenceable": "1", "520 Shutter-Demo": "No", "528 Shutter-Demo": "No", "470-Name": "DA Shutter", "520-DA Device": "520 Shutter", "XYStage-Description": "Ludl XY stage driver adapter", "445 Shutter-MinVolts": "0.0000", "528 AO-TriggerSampleRate": "100000", "405 AO-MinVolts": "0.0000", "405 Shutter-TriggerSequenceLength": "1000", "Height": 1200, "555 AO-Block voltage": "false", "640 Shutter-Volts": "0.0000", "PixelType": "GRAY16", "445 Shutter-Block voltage": "false", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "520 Shutter-TriggerSequenceLength": "1000", "COM9-Name": "COM9", "Core-SLM": "", "555 AO-Volts": "4.5000", "555 Shutter-Block voltage": "false", "XYStage-Speed": "1800.0000", "520 AO-MaxVolts": "5.0000", "528 Shutter-SupportsTriggering": "0", "COM1-Description": "Serial port driver (boost:asio)", "PRIME-MetadataEnabled": "No", "520 Shutter-MinVolts": "0.0000", "470 Shutter-Sequenceable": "1", "PRIME-PreampDelay": "0", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-MinVolts": "0.0000", "ChannelIndex": 1, "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Core-AutoShutter": "1", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "Exposure-ms": 160, "PRIME-PP 2 ENABLED": "Yes", "640 Shutter-TriggerSampleRate": "100000", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "470 Shutter-IOChannel": "Dev1/ao10", "405 AO-Demo": "No", "PixelSizeUm": 1.1, "470 AO-Demo": "No", "470 Shutter-MaxVolts": "5.0000", "Vincent-D3-Name": "Vincent-D3", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FileName": "img_000000000_Il-T_007.tif", "LudlController-Reset": "Operate", "PRIME-Exposure": "160.00", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "445-Name": "DA Shutter", "555 Shutter-Name": "AnalogIO", "520 Shutter-TriggerSampleRate": "100000", "445 Shutter-IOChannel": "Dev1/ao9", "PRIME-TransposeCorrection": "0", "XYStage-StepSize": "0.1000", "445-Description": "DA device that is used as a shutter", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 AO-IOChannel": "Dev1/ao7", "520 Shutter-Name": "AnalogIO", "COM3-DataBits": "8", "640 AO-Volts": "0.0000", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "555 Shutter-IOChannel": "Dev1/ao13", "WaitInterval": null, "520 Shutter-Sequenceable": "1", "YPositionUm": 31506.2, "445-State": "0", "445-DA Device": "445 Shutter", "PRIME-CircularBufferAutoSize": "ON", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "555 AO-TriggerSampleRate": "100000", "445 Shutter-Volts": "0.0000", "XLIGHT Hub-Port": "COM3", "Core-AutoFocus": "", "COM4-Handshaking": "Off", "528 Shutter-MinVolts": "0.0000", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "445 Shutter-Name": "AnalogIO", "528 Shutter-Sequenceable": "1", "XYStage-TransposeMirrorX": "0", "XYStage-TransposeMirrorY": "0", "PRIME-ReadoutRate": "100MHz 16bit", "XYStage-Name": "Stage", "640 AO-SupportsTriggering": "0", "640-Name": "DA Shutter", "640 Shutter-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "640 AO-Block voltage": "false", "445 Shutter-Sequenceable": "1", "470 AO-MaxVolts": "5.0000", "COM4-BaudRate": "9600", "470-Description": "DA device that is used as a shutter", "520 Shutter-Description": "NI DAC", "PRIME-CCDTemperature": "-14.8500", "Time": "2019-12-18 21:36:52 -0500", "640 AO-TriggerSequenceLength": "1000", "470 AO-Name": "AnalogIO", "XLIGHT Disk slider-Label": "Disk Pos 40um", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "Binning": "1x1", "TE2000-ModelType": "1", "COM3-AnswerTimeout": "500.0000", "555 Shutter-SupportsTriggering": "0", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "TE2000-Port": "COM4", "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Description": "NI DAC", "COM4-AnswerTimeout": "500.0000", "COM1-DelayBetweenCharsMs": "0.0000", "FilterBlock-Label": "Confocal", "445 AO-Name": "AnalogIO", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-Timing-ReadoutTimeNs": "24672000.0000", "528 AO-Demo": "No", "520 AO-Sequenceable": "1", "555-Description": "DA device that is used as a shutter", "405-DA Device": "405 Shutter", "405 AO-TriggerSequenceLength": "1000", "640 AO-MinVolts": "0.0000", "640 Shutter-Block voltage": "false", "FrameIndex": 0, "PRIME-Port": "CMOS", "555 AO-MinVolts": "0.0000", "COM3-Description": "Serial port driver (boost:asio)", "528 AO-Description": "NI DAC", "555 AO-IOChannel": "Dev1/ao6", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "555 AO-TriggerInputLine": "/Dev1/PFI0", "PRIME-ActualInterval-ms": "390.5200", "LudlController-Port": "COM9", "445 Shutter-Demo": "No", "PRIME-Trigger-Expose Out-Mux": "1", "SliceIndex": 7, "PRIME-CCDTemperatureSetPoint": "-15.0000", "405 Shutter-MinVolts": "0.0000", "PRIME-ExposeOutMode": "First Row", "AxisPositions": null, "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Volts": "5.0000", "528-Name": "DA Shutter", "555-State": "1", "XLIGHT Dichroic-Label": "QUAD", "445 AO-Sequenceable": "1", "405-Name": "DA Shutter", "405 AO-IOChannel": "Dev1/ao0", "Core-Camera": "PRIME", "Focus-Name": "Focus", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "405 Shutter-IOChannel": "Dev1/ao8", "640 AO-Demo": "No", "640 Shutter-SupportsTriggering": "0", "445 AO-Demo": "No", "555-DA Device": "555 Shutter", "470 Shutter-Description": "NI DAC", "528 AO-Volts": "0.0000", "COM1-DataBits": "8", "Vincent-D3-Address": "x", "520 AO-SupportsTriggering": "0", "405-Description": "DA device that is used as a shutter", "640 Shutter-MinVolts": "0.0000", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "405 AO-Name": "AnalogIO", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "640 AO-TriggerInputLine": "/Dev1/PFI0", "405 AO-TriggerInputLine": "/Dev1/PFI0", "640-Description": "DA device that is used as a shutter", "445 Shutter-TriggerSampleRate": "100000", "470 Shutter-TriggerSequenceLength": "1000", "COM1-StopBits": "1", "520 Shutter-SupportsTriggering": "0", "405-State": "0", "Core-Initialize": "1", "XLIGHT Emission Wheel-State": "3", "520 AO-Description": "NI DAC", "528 AO-TriggerInputLine": "/Dev1/PFI0", "445 Shutter-MaxVolts": "5.0000", "COM9-Handshaking": "Off", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "XLIGHT Spin motor-Label": "On (spinning)", "PRIME-PP 5 ENABLED": "No", "UUID": "d69745b1-31e4-4442-8207-b13feeb7c446", "PRIME-TriggerMode": "Internal Trigger", "COM1-Handshaking": "Off", "PRIME-Timing-PostTriggerDelayNs": "49344000.0000", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "Focus-Position": "17741.5000", "528 Shutter-Block voltage": "false", "Channel": "Il-T", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "555 Shutter-Demo": "No", "405 Shutter-Demo": "No", "528 AO-Name": "AnalogIO", "520-State": "0", "Core-Galvo": "", "405 Shutter-TriggerSampleRate": "100000", "Focus-FocusStepSize_(nm)": "100", "PRIME-PixelType": "16bit", "COM4-DataBits": "8", "470 AO-IOChannel": "Dev1/ao2", "520 AO-MinVolts": "0.0000", "COM9-Parity": "None", "PRIME-CircularBufferEnabled": "ON", "PRIME-PP 6 Reset": "No", "640 AO-Description": "NI DAC", "Source": "PRIME", "445 AO-MinVolts": "0.0000", "PIEZO-Name": "Stage", "445 AO-Description": "NI DAC", "PRIME-CircularBufferFrameCount": "46", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "520 AO-Volts": "0.0000", "528 Shutter-TriggerSampleRate": "100000", "XPositionUm": 19731.9, "OpticalPath-State": "4", "PRIME-PP 3 THRESHOLD": "80", "PRIME-PVCAM Version": "3.7.1", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Gain": "1-HDR", "640 Shutter-TriggerSequenceLength": "1000", "528 AO-SupportsTriggering": "0", "445 AO-TriggerInputLine": "/Dev1/PFI0", "OpticalPath-Name": "OpticalPath", "PRIME-PP 4 ENABLED": "Yes", "PRIME-PVCAM Adapter Version": "1.3.29", "COM3-Name": "COM3", "405 AO-MaxVolts": "5.0000", "520 Shutter-Block voltage": "false", "PRIME-Timing-ClearingTimeNs": "49344000.0000", "Core-Focus": "PIEZO", "528 AO-Block voltage": "false", "TE2000-Description": "Nikon TE2000 hub", "528 AO-MinVolts": "0.0000", "COM4-Parity": "None", "Summary": { "Slices": 11, "ChContrastMin": [ 0, 0, 0, 0, 0, 0 ], "PixelAspect": 1, "SlicesFirst": true, "Interval_ms": 0, "Time": "2019-12-18 21:36:42 -0500", "Prefix": "seq_4", "InitialPositionList": [ { "Label": "Pos0", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.21], "Focus": [17741.5], "XYStage": [ 44542.200000000004, 31636.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos1", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.22], "Focus": [17741.4], "XYStage": [ 43801.100000000006, 31637.9 ] }, "GridColumnIndex": 0 }, { "Label": "Pos2", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.5], "XYStage": [ 38561.3, 32040.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos3", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.185], "Focus": [17741.3], "XYStage": [ 37698.4, 32041.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos4", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.16], "Focus": [17741.5], "XYStage": [ 32375.4, 31758.9 ] }, "GridColumnIndex": 0 }, { "Label": "Pos5", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.17000000000002], "Focus": [17741.3], "XYStage": [ 31770.7, 31758.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos6", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.4], "XYStage": [ 26536.800000000003, 31068.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos7", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.205], "Focus": [17741.4], "XYStage": [ 25420.2, 31068.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos8", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.85], "Focus": [17741.3], "XYStage": [ 21059, 31508 ] }, "GridColumnIndex": 0 }, { "Label": "Pos9", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.82], "Focus": [17741.3], "XYStage": [ 19731.9, 31506.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos10", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.79], "Focus": [17741.4], "XYStage": [ 14528.5, 32007.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos11", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.83], "Focus": [17741.2], "XYStage": [ 13830.300000000001, 32009.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos12", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.79], "Focus": [17741.3], "XYStage": [ 8775.300000000001, 32017.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos13", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.815], "Focus": [17741.3], "XYStage": [ 8109.200000000001, 32008.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos14", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.805], "Focus": [17741.3], "XYStage": [ 3483.3, 31908.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos15", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.82], "Focus": [17741.2], "XYStage": [ 2686.8, 31903.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos16", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [211.48000000000002], "Focus": [17741.1], "XYStage": [ -1978.9, 31973.600000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos17", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [211.51500000000001], "Focus": [17741.1], "XYStage": [ -2957.8, 31982.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos18", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.195], "Focus": [17741.5], "XYStage": [ 47747.4, -576.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos19", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.23000000000002], "Focus": [17741.6], "XYStage": [ 47053.9, -576.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos20", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.205], "Focus": [17741.4], "XYStage": [ 41871.200000000004, -479.8 ] }, "GridColumnIndex": 0 }, { "Label": "Pos21", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.4], "XYStage": [ 40882.600000000006, -392 ] }, "GridColumnIndex": 0 }, { "Label": "Pos22", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.24], "Focus": [17741.4], "XYStage": [ 35905.6, -135.3 ] }, "GridColumnIndex": 0 }, { "Label": "Pos23", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.195], "Focus": [17741.5], "XYStage": [ 34990.9, -132.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos24", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.46], "Focus": [17741.4], "XYStage": [ 29678.100000000002, -958.1 ] }, "GridColumnIndex": 0 }, { "Label": "Pos25", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.44], "Focus": [17741.5], "XYStage": [ 28983.600000000002, -939.4000000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos26", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.475], "Focus": [17741.5], "XYStage": [ 23019.7, -122 ] }, "GridColumnIndex": 0 }, { "Label": "Pos27", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.44], "Focus": [17741.7], "XYStage": [ 22428, -116.80000000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos28", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.46], "Focus": [17741.7], "XYStage": [ 16364.900000000001, -181.10000000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos29", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.48000000000002], "Focus": [17741.5], "XYStage": [ 15712.1, -180 ] }, "GridColumnIndex": 0 }, { "Label": "Pos30", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.45000000000002], "Focus": [17741.7], "XYStage": [ 10346.1, 77.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos31", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.49], "Focus": [17741.4], "XYStage": [ 9755.5, 85.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos32", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.475], "Focus": [17741.5], "XYStage": [ 4775.5, 293.90000000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos33", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.445], "Focus": [17741.4], "XYStage": [ 3954.9, 293.90000000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos34", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.455], "Focus": [17741.4], "XYStage": [ -1324.9, 545.6 ] }, "GridColumnIndex": 0 }, { "Label": "Pos35", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.445], "Focus": [17741.3], "XYStage": [ -2097, 576.6 ] }, "GridColumnIndex": 0 } ], "z-step_um": 10, "GridRow": 0, "BitDepth": 16, "Depth": 2, "Channels": 6, "Source": "Micro-Manager", "ChColors": [ -16711681, -256, -65281, -1, -1, -16776961 ], "MetadataVersion": 10, "KeepShutterOpenChannels": true, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536, 65536 ], "CustomIntervals_ms": [], "Height": 1200, "MicroManagerVersion": "1.4.23 20180219", "GridColumn": 0, "UUID": "14a13bf7-c944-4672-b873-fcfbbb7ea13d", "PixelType": "GRAY16", "Width": 1200, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\Yan\\20191218-YC113-S7S8-miseq", "Frames": 1, "Comment": "", "UserName": "Nikon", "PixelSize_um": 1.1, "ROI": [ 0, 0, 1200, 1200 ], "Date": "2019-12-18", "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC", "P-DAPI-high" ], "KeepShutterOpenSlices": true, "IJType": 1, "ComputerName": "ZadorConfocal2", "Positions": 36, "PositionIndex": 9, "CameraTimeout": 20000, "TimeFirst": false }, "640 AO-Name": "AnalogIO", "Vincent-D3-Command": "Close" }, "FrameKey-0-1-8": { "COM4-StopBits": "1", "528 AO-MaxVolts": "5.0000", "XLIGHT Excitation Wheel-State": "1", "470 Shutter-Name": "AnalogIO", "Core-Shutter": "555", "555 AO-Demo": "No", "XLIGHT Dichroic-State": "2", "XYStage-StepSize-Y": "0.1000", "ZPositionUm": 278.82, "XYStage-StepSize-X": "0.1000", "PRIME-FirmwareVersion": "20.28", "PRIME-ClearCycles": "2", "520-Name": "DA Shutter", "405 Shutter-Name": "AnalogIO", "PIEZO-StepSize": "0.0050", "PRIME-ClearMode": "Pre-Exposure", "470 AO-MinVolts": "0.0000", "405 AO-SupportsTriggering": "1", "CameraChannelIndex": 0, "XYStage-StartSpeed": "500.0000", "COM9-StopBits": "2", "405 AO-Description": "NI DAC", "COM4-DelayBetweenCharsMs": "0.0000", "445 AO-Block voltage": "false", "520 AO-TriggerInputLine": "/Dev1/PFI0", "555 AO-Description": "NI DAC", "520 AO-TriggerSampleRate": "100000", "COM4-Verbose": "1", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM4-Name": "COM4", "405 Shutter-Volts": "0.0000", "Vincent-D3-Port": "COM1", "PRIME-PP 4 MIN ADU AFFECTED": "200", "470 Shutter-MinVolts": "0.0000", "PRIME-PP 3 ENABLED": "Yes", "470 AO-Description": "NI DAC", "PRIME-Description": "PVCAM API device adapter", "520 Shutter-MaxVolts": "5.0000", "520 Shutter-Volts": "0.0000", "528 AO-Sequenceable": "1", "PRIME-Binning": "1x1", "555 Shutter-MaxVolts": "5.0000", "528 Shutter-TriggerSequenceLength": "1000", "405 Shutter-Block voltage": "false", "XLIGHT Spin motor-State": "1", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "Vincent-D3-Time to open (ms)": "35.0000", "Vincent-D3-Time to close (ms)": "35.0000", "445 AO-TriggerSampleRate": "100000", "640 Shutter-IOChannel": "Dev1/ao14", "555 AO-Sequenceable": "1", "520 AO-Name": "AnalogIO", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "470 AO-Block voltage": "false", "COM1-Verbose": "1", "COM9-AnswerTimeout": "10000.0000", "528 Shutter-Name": "AnalogIO", "PRIME-Trigger Timeout (secs)": "10", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "NextFrame": 0, "470 AO-TriggerSequenceLength": "1000", "PRIME-TransposeXY": "0", "445 AO-TriggerSequenceLength": "1000", "COM1-Name": "COM1", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "COM9-DataBits": "8", "COM1-AnswerTimeout": "500.0000", "Camera": "", "TE2000-Version": "V2.50-V2.50-V2.10", "528 Shutter-Volts": "0.0000", "XLIGHT Hub-Name": "XLIGHT Hub", "PIEZO-Description": "Ludl stage driver adapter", "PositionName": "Pos9", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSampleRate": "100000", "PRIME-FanSpeedSetpoint": "High", "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "405 AO-TriggerSampleRate": "100000", "470 Shutter-Block voltage": "false", "470-State": "0", "COM3-Parity": "None", "405 AO-Volts": "0.0000", "PRIME-TransposeMirrorY": "0", "PRIME-ChipName": "GS144BSI", "470 AO-Volts": "0.0000", "PRIME-TransposeMirrorX": "0", "445 Shutter-SupportsTriggering": "0", "470 Shutter-SupportsTriggering": "0", "XLIGHT Hub-Description": "XLIGHT hub", "PRIME-PreampOffLimit": "10000", "470 AO-Sequenceable": "1", "528 Shutter-MaxVolts": "5.0000", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "Core-XYStage": "XYStage", "470 AO-TriggerSampleRate": "100000", "PRIME-Timing-PreTriggerDelayNs": "24672000.0000", "528-DA Device": "528 Shutter", "Focus-Description": "Nikon TE2000 focus adapter", "FilterBlock-State": "5", "520 AO-Demo": "No", "COM3-DelayBetweenCharsMs": "0.0000", "470 Shutter-Volts": "0.0000", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "PRIME-PP 2 THRESHOLD": "125", "PRIME-PP 4 THRESHOLD": "75", "XYStage-Acceleration": "4.0000", "470 Shutter-Demo": "No", "PRIME-Name": "PMPCIECam00", "640-DA Device": "640 Shutter", "405 AO-Block voltage": "false", "ElapsedTime-ms": 384837, "PRIME-Offset": "100", "445 AO-IOChannel": "Dev1/ao1", "640 AO-MaxVolts": "5.0000", "PositionIndex": 9, "PRIME-PP 1 ENABLED": "Yes", "COM1-BaudRate": "9600", "640-State": "0", "COM3-Verbose": "1", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "LudlController-TransmissionDelay": "0", "PRIME-FullWellCapacity": "62000", "PRIME-SMARTStreamingEnabled": "No", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "640 Shutter-Description": "NI DAC", "528-Description": "DA device that is used as a shutter", "COM4-Description": "Serial port driver (boost:asio)", "405 Shutter-Description": "NI DAC", "TE2000-Name": "TE2000", "528 Shutter-Description": "NI DAC", "COM9-Description": "Serial port driver (boost:asio)", "LudlController-Name": "LudlController", "445 Shutter-TriggerSequenceLength": "1000", "PRIME-SerialNumber": "A18B203008", "445 AO-Volts": "0.0000", "PRIME-X-dimension": "1200", "PRIME-ShutterCloseDelay": "0", "XLIGHT Disk slider-State": "2", "Position": "Default", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 AO-IOChannel": "Dev1/ao5", "520 AO-IOChannel": "Dev1/ao4", "528 AO-TriggerSequenceLength": "1000", "405 Shutter-MaxVolts": "5.0000", "470-DA Device": "470 Shutter", "PRIME-FTCapable": "No", "Core-ChannelGroup": "Channels", "555 AO-SupportsTriggering": "0", "PRIME-Y-dimension": "1200", "Frame": 0, "555 Shutter-Sequenceable": "1", "PRIME-Timing-ExposureTimeNs": "499998000.0000", "Core-ImageProcessor": "", "528-State": "0", "520 AO-Block voltage": "false", "XLIGHT Excitation Wheel-Label": "QUAD", "640 Shutter-Demo": "No", "COM3-StopBits": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "520 Shutter-IOChannel": "Dev1/ao11", "Width": 1200, "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-SupportsTriggering": "0", "640 AO-TriggerSampleRate": "100000", "520-Description": "DA device that is used as a shutter", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "405 Shutter-Sequenceable": "1", "405 Shutter-SupportsTriggering": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-TriggerSampleRate": "100000", "640 Shutter-MaxVolts": "5.0000", "555 AO-MaxVolts": "5.0000", "520 AO-TriggerSequenceLength": "1000", "COM3-BaudRate": "9600", "COM9-BaudRate": "9600", "Vincent-D3-Channel #": "Ch. #1", "PRIME-PMode": "Normal", "OpticalPath-Label": "Left(100)", "BitDepth": 16, "555 AO-TriggerSequenceLength": "1000", "445 Shutter-Description": "NI DAC", "405 AO-Sequenceable": "1", "PRIME-AcquisitionMethod": "Callbacks", "555 AO-Name": "AnalogIO", "PRIME-ShutterMode": "Pre-Exposure", "COM3-Handshaking": "Off", "445 AO-SupportsTriggering": "0", "ROI": "0-0-1200-1200", "COM9-Verbose": "1", "PRIME-Color": "OFF", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "PRIME-ShutterOpenDelay": "0", "COM1-Parity": "None", "Slice": 8, "SlicePosition": 30, "COM9-DelayBetweenCharsMs": "0.0000", "555-Name": "DA Shutter", "PIEZO-LudlSingleAxisName": "B", "XLIGHT Emission Wheel-Label": "585/11(T)", "640 Shutter-Name": "AnalogIO", "640 AO-Sequenceable": "1", "520 Shutter-Demo": "No", "528 Shutter-Demo": "No", "470-Name": "DA Shutter", "520-DA Device": "520 Shutter", "XYStage-Description": "Ludl XY stage driver adapter", "445 Shutter-MinVolts": "0.0000", "528 AO-TriggerSampleRate": "100000", "405 AO-MinVolts": "0.0000", "405 Shutter-TriggerSequenceLength": "1000", "Height": 1200, "555 AO-Block voltage": "false", "640 Shutter-Volts": "0.0000", "PixelType": "GRAY16", "445 Shutter-Block voltage": "false", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "520 Shutter-TriggerSequenceLength": "1000", "COM9-Name": "COM9", "Core-SLM": "", "555 AO-Volts": "4.5000", "555 Shutter-Block voltage": "false", "XYStage-Speed": "1800.0000", "520 AO-MaxVolts": "5.0000", "528 Shutter-SupportsTriggering": "0", "COM1-Description": "Serial port driver (boost:asio)", "PRIME-MetadataEnabled": "No", "520 Shutter-MinVolts": "0.0000", "470 Shutter-Sequenceable": "1", "PRIME-PreampDelay": "0", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-MinVolts": "0.0000", "ChannelIndex": 1, "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Core-AutoShutter": "1", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "Exposure-ms": 160, "PRIME-PP 2 ENABLED": "Yes", "640 Shutter-TriggerSampleRate": "100000", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "470 Shutter-IOChannel": "Dev1/ao10", "405 AO-Demo": "No", "PixelSizeUm": 1.1, "470 AO-Demo": "No", "470 Shutter-MaxVolts": "5.0000", "Vincent-D3-Name": "Vincent-D3", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FileName": "img_000000000_Il-T_008.tif", "LudlController-Reset": "Operate", "PRIME-Exposure": "160.00", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "445-Name": "DA Shutter", "555 Shutter-Name": "AnalogIO", "520 Shutter-TriggerSampleRate": "100000", "445 Shutter-IOChannel": "Dev1/ao9", "PRIME-TransposeCorrection": "0", "XYStage-StepSize": "0.1000", "445-Description": "DA device that is used as a shutter", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 AO-IOChannel": "Dev1/ao7", "520 Shutter-Name": "AnalogIO", "COM3-DataBits": "8", "640 AO-Volts": "0.0000", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "555 Shutter-IOChannel": "Dev1/ao13", "WaitInterval": null, "520 Shutter-Sequenceable": "1", "YPositionUm": 31506.2, "445-State": "0", "445-DA Device": "445 Shutter", "PRIME-CircularBufferAutoSize": "ON", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "555 AO-TriggerSampleRate": "100000", "445 Shutter-Volts": "0.0000", "XLIGHT Hub-Port": "COM3", "Core-AutoFocus": "", "COM4-Handshaking": "Off", "528 Shutter-MinVolts": "0.0000", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "445 Shutter-Name": "AnalogIO", "528 Shutter-Sequenceable": "1", "XYStage-TransposeMirrorX": "0", "XYStage-TransposeMirrorY": "0", "PRIME-ReadoutRate": "100MHz 16bit", "XYStage-Name": "Stage", "640 AO-SupportsTriggering": "0", "640-Name": "DA Shutter", "640 Shutter-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "640 AO-Block voltage": "false", "445 Shutter-Sequenceable": "1", "470 AO-MaxVolts": "5.0000", "COM4-BaudRate": "9600", "470-Description": "DA device that is used as a shutter", "520 Shutter-Description": "NI DAC", "PRIME-CCDTemperature": "-14.8500", "Time": "2019-12-18 21:36:52 -0500", "640 AO-TriggerSequenceLength": "1000", "470 AO-Name": "AnalogIO", "XLIGHT Disk slider-Label": "Disk Pos 40um", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "Binning": "1x1", "TE2000-ModelType": "1", "COM3-AnswerTimeout": "500.0000", "555 Shutter-SupportsTriggering": "0", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "TE2000-Port": "COM4", "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Description": "NI DAC", "COM4-AnswerTimeout": "500.0000", "COM1-DelayBetweenCharsMs": "0.0000", "FilterBlock-Label": "Confocal", "445 AO-Name": "AnalogIO", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-Timing-ReadoutTimeNs": "24672000.0000", "528 AO-Demo": "No", "520 AO-Sequenceable": "1", "555-Description": "DA device that is used as a shutter", "405-DA Device": "405 Shutter", "405 AO-TriggerSequenceLength": "1000", "640 AO-MinVolts": "0.0000", "640 Shutter-Block voltage": "false", "FrameIndex": 0, "PRIME-Port": "CMOS", "555 AO-MinVolts": "0.0000", "COM3-Description": "Serial port driver (boost:asio)", "528 AO-Description": "NI DAC", "555 AO-IOChannel": "Dev1/ao6", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "555 AO-TriggerInputLine": "/Dev1/PFI0", "PRIME-ActualInterval-ms": "390.5200", "LudlController-Port": "COM9", "445 Shutter-Demo": "No", "PRIME-Trigger-Expose Out-Mux": "1", "SliceIndex": 8, "PRIME-CCDTemperatureSetPoint": "-15.0000", "405 Shutter-MinVolts": "0.0000", "PRIME-ExposeOutMode": "First Row", "AxisPositions": null, "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Volts": "5.0000", "528-Name": "DA Shutter", "555-State": "1", "XLIGHT Dichroic-Label": "QUAD", "445 AO-Sequenceable": "1", "405-Name": "DA Shutter", "405 AO-IOChannel": "Dev1/ao0", "Core-Camera": "PRIME", "Focus-Name": "Focus", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "405 Shutter-IOChannel": "Dev1/ao8", "640 AO-Demo": "No", "640 Shutter-SupportsTriggering": "0", "445 AO-Demo": "No", "555-DA Device": "555 Shutter", "470 Shutter-Description": "NI DAC", "528 AO-Volts": "0.0000", "COM1-DataBits": "8", "Vincent-D3-Address": "x", "520 AO-SupportsTriggering": "0", "405-Description": "DA device that is used as a shutter", "640 Shutter-MinVolts": "0.0000", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "405 AO-Name": "AnalogIO", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "640 AO-TriggerInputLine": "/Dev1/PFI0", "405 AO-TriggerInputLine": "/Dev1/PFI0", "640-Description": "DA device that is used as a shutter", "445 Shutter-TriggerSampleRate": "100000", "470 Shutter-TriggerSequenceLength": "1000", "COM1-StopBits": "1", "520 Shutter-SupportsTriggering": "0", "405-State": "0", "Core-Initialize": "1", "XLIGHT Emission Wheel-State": "3", "520 AO-Description": "NI DAC", "528 AO-TriggerInputLine": "/Dev1/PFI0", "445 Shutter-MaxVolts": "5.0000", "COM9-Handshaking": "Off", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "XLIGHT Spin motor-Label": "On (spinning)", "PRIME-PP 5 ENABLED": "No", "UUID": "3f5698e4-a58f-46b4-a7b5-bd681544a018", "PRIME-TriggerMode": "Internal Trigger", "COM1-Handshaking": "Off", "PRIME-Timing-PostTriggerDelayNs": "49344000.0000", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "Focus-Position": "17741.5000", "528 Shutter-Block voltage": "false", "Channel": "Il-T", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "555 Shutter-Demo": "No", "405 Shutter-Demo": "No", "528 AO-Name": "AnalogIO", "520-State": "0", "Core-Galvo": "", "405 Shutter-TriggerSampleRate": "100000", "Focus-FocusStepSize_(nm)": "100", "PRIME-PixelType": "16bit", "COM4-DataBits": "8", "470 AO-IOChannel": "Dev1/ao2", "520 AO-MinVolts": "0.0000", "COM9-Parity": "None", "PRIME-CircularBufferEnabled": "ON", "PRIME-PP 6 Reset": "No", "640 AO-Description": "NI DAC", "Source": "PRIME", "445 AO-MinVolts": "0.0000", "PIEZO-Name": "Stage", "445 AO-Description": "NI DAC", "PRIME-CircularBufferFrameCount": "46", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "520 AO-Volts": "0.0000", "528 Shutter-TriggerSampleRate": "100000", "XPositionUm": 19731.9, "OpticalPath-State": "4", "PRIME-PP 3 THRESHOLD": "80", "PRIME-PVCAM Version": "3.7.1", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Gain": "1-HDR", "640 Shutter-TriggerSequenceLength": "1000", "528 AO-SupportsTriggering": "0", "445 AO-TriggerInputLine": "/Dev1/PFI0", "OpticalPath-Name": "OpticalPath", "PRIME-PP 4 ENABLED": "Yes", "PRIME-PVCAM Adapter Version": "1.3.29", "COM3-Name": "COM3", "405 AO-MaxVolts": "5.0000", "520 Shutter-Block voltage": "false", "PRIME-Timing-ClearingTimeNs": "49344000.0000", "Core-Focus": "PIEZO", "528 AO-Block voltage": "false", "TE2000-Description": "Nikon TE2000 hub", "528 AO-MinVolts": "0.0000", "COM4-Parity": "None", "Summary": { "Slices": 11, "ChContrastMin": [ 0, 0, 0, 0, 0, 0 ], "PixelAspect": 1, "SlicesFirst": true, "Interval_ms": 0, "Time": "2019-12-18 21:36:42 -0500", "Prefix": "seq_4", "InitialPositionList": [ { "Label": "Pos0", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.21], "Focus": [17741.5], "XYStage": [ 44542.200000000004, 31636.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos1", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.22], "Focus": [17741.4], "XYStage": [ 43801.100000000006, 31637.9 ] }, "GridColumnIndex": 0 }, { "Label": "Pos2", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.5], "XYStage": [ 38561.3, 32040.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos3", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.185], "Focus": [17741.3], "XYStage": [ 37698.4, 32041.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos4", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.16], "Focus": [17741.5], "XYStage": [ 32375.4, 31758.9 ] }, "GridColumnIndex": 0 }, { "Label": "Pos5", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.17000000000002], "Focus": [17741.3], "XYStage": [ 31770.7, 31758.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos6", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.4], "XYStage": [ 26536.800000000003, 31068.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos7", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.205], "Focus": [17741.4], "XYStage": [ 25420.2, 31068.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos8", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.85], "Focus": [17741.3], "XYStage": [ 21059, 31508 ] }, "GridColumnIndex": 0 }, { "Label": "Pos9", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.82], "Focus": [17741.3], "XYStage": [ 19731.9, 31506.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos10", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.79], "Focus": [17741.4], "XYStage": [ 14528.5, 32007.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos11", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.83], "Focus": [17741.2], "XYStage": [ 13830.300000000001, 32009.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos12", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.79], "Focus": [17741.3], "XYStage": [ 8775.300000000001, 32017.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos13", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.815], "Focus": [17741.3], "XYStage": [ 8109.200000000001, 32008.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos14", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.805], "Focus": [17741.3], "XYStage": [ 3483.3, 31908.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos15", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.82], "Focus": [17741.2], "XYStage": [ 2686.8, 31903.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos16", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [211.48000000000002], "Focus": [17741.1], "XYStage": [ -1978.9, 31973.600000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos17", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [211.51500000000001], "Focus": [17741.1], "XYStage": [ -2957.8, 31982.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos18", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.195], "Focus": [17741.5], "XYStage": [ 47747.4, -576.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos19", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.23000000000002], "Focus": [17741.6], "XYStage": [ 47053.9, -576.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos20", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.205], "Focus": [17741.4], "XYStage": [ 41871.200000000004, -479.8 ] }, "GridColumnIndex": 0 }, { "Label": "Pos21", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.4], "XYStage": [ 40882.600000000006, -392 ] }, "GridColumnIndex": 0 }, { "Label": "Pos22", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.24], "Focus": [17741.4], "XYStage": [ 35905.6, -135.3 ] }, "GridColumnIndex": 0 }, { "Label": "Pos23", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.195], "Focus": [17741.5], "XYStage": [ 34990.9, -132.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos24", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.46], "Focus": [17741.4], "XYStage": [ 29678.100000000002, -958.1 ] }, "GridColumnIndex": 0 }, { "Label": "Pos25", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.44], "Focus": [17741.5], "XYStage": [ 28983.600000000002, -939.4000000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos26", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.475], "Focus": [17741.5], "XYStage": [ 23019.7, -122 ] }, "GridColumnIndex": 0 }, { "Label": "Pos27", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.44], "Focus": [17741.7], "XYStage": [ 22428, -116.80000000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos28", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.46], "Focus": [17741.7], "XYStage": [ 16364.900000000001, -181.10000000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos29", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.48000000000002], "Focus": [17741.5], "XYStage": [ 15712.1, -180 ] }, "GridColumnIndex": 0 }, { "Label": "Pos30", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.45000000000002], "Focus": [17741.7], "XYStage": [ 10346.1, 77.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos31", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.49], "Focus": [17741.4], "XYStage": [ 9755.5, 85.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos32", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.475], "Focus": [17741.5], "XYStage": [ 4775.5, 293.90000000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos33", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.445], "Focus": [17741.4], "XYStage": [ 3954.9, 293.90000000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos34", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.455], "Focus": [17741.4], "XYStage": [ -1324.9, 545.6 ] }, "GridColumnIndex": 0 }, { "Label": "Pos35", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.445], "Focus": [17741.3], "XYStage": [ -2097, 576.6 ] }, "GridColumnIndex": 0 } ], "z-step_um": 10, "GridRow": 0, "BitDepth": 16, "Depth": 2, "Channels": 6, "Source": "Micro-Manager", "ChColors": [ -16711681, -256, -65281, -1, -1, -16776961 ], "MetadataVersion": 10, "KeepShutterOpenChannels": true, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536, 65536 ], "CustomIntervals_ms": [], "Height": 1200, "MicroManagerVersion": "1.4.23 20180219", "GridColumn": 0, "UUID": "14a13bf7-c944-4672-b873-fcfbbb7ea13d", "PixelType": "GRAY16", "Width": 1200, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\Yan\\20191218-YC113-S7S8-miseq", "Frames": 1, "Comment": "", "UserName": "Nikon", "PixelSize_um": 1.1, "ROI": [ 0, 0, 1200, 1200 ], "Date": "2019-12-18", "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC", "P-DAPI-high" ], "KeepShutterOpenSlices": true, "IJType": 1, "ComputerName": "ZadorConfocal2", "Positions": 36, "PositionIndex": 9, "CameraTimeout": 20000, "TimeFirst": false }, "640 AO-Name": "AnalogIO", "Vincent-D3-Command": "Close" }, "FrameKey-0-1-9": { "COM4-StopBits": "1", "528 AO-MaxVolts": "5.0000", "XLIGHT Excitation Wheel-State": "1", "470 Shutter-Name": "AnalogIO", "Core-Shutter": "555", "555 AO-Demo": "No", "XLIGHT Dichroic-State": "2", "XYStage-StepSize-Y": "0.1000", "ZPositionUm": 288.82, "XYStage-StepSize-X": "0.1000", "PRIME-FirmwareVersion": "20.28", "PRIME-ClearCycles": "2", "520-Name": "DA Shutter", "405 Shutter-Name": "AnalogIO", "PIEZO-StepSize": "0.0050", "PRIME-ClearMode": "Pre-Exposure", "470 AO-MinVolts": "0.0000", "405 AO-SupportsTriggering": "1", "CameraChannelIndex": 0, "XYStage-StartSpeed": "500.0000", "COM9-StopBits": "2", "405 AO-Description": "NI DAC", "COM4-DelayBetweenCharsMs": "0.0000", "445 AO-Block voltage": "false", "520 AO-TriggerInputLine": "/Dev1/PFI0", "555 AO-Description": "NI DAC", "520 AO-TriggerSampleRate": "100000", "COM4-Verbose": "1", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM4-Name": "COM4", "405 Shutter-Volts": "0.0000", "Vincent-D3-Port": "COM1", "PRIME-PP 4 MIN ADU AFFECTED": "200", "470 Shutter-MinVolts": "0.0000", "PRIME-PP 3 ENABLED": "Yes", "470 AO-Description": "NI DAC", "PRIME-Description": "PVCAM API device adapter", "520 Shutter-MaxVolts": "5.0000", "520 Shutter-Volts": "0.0000", "528 AO-Sequenceable": "1", "PRIME-Binning": "1x1", "555 Shutter-MaxVolts": "5.0000", "528 Shutter-TriggerSequenceLength": "1000", "405 Shutter-Block voltage": "false", "XLIGHT Spin motor-State": "1", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "Vincent-D3-Time to open (ms)": "35.0000", "Vincent-D3-Time to close (ms)": "35.0000", "445 AO-TriggerSampleRate": "100000", "640 Shutter-IOChannel": "Dev1/ao14", "555 AO-Sequenceable": "1", "520 AO-Name": "AnalogIO", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "470 AO-Block voltage": "false", "COM1-Verbose": "1", "COM9-AnswerTimeout": "10000.0000", "528 Shutter-Name": "AnalogIO", "PRIME-Trigger Timeout (secs)": "10", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "NextFrame": 0, "470 AO-TriggerSequenceLength": "1000", "PRIME-TransposeXY": "0", "445 AO-TriggerSequenceLength": "1000", "COM1-Name": "COM1", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "COM9-DataBits": "8", "COM1-AnswerTimeout": "500.0000", "Camera": "", "TE2000-Version": "V2.50-V2.50-V2.10", "528 Shutter-Volts": "0.0000", "XLIGHT Hub-Name": "XLIGHT Hub", "PIEZO-Description": "Ludl stage driver adapter", "PositionName": "Pos9", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSampleRate": "100000", "PRIME-FanSpeedSetpoint": "High", "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "405 AO-TriggerSampleRate": "100000", "470 Shutter-Block voltage": "false", "470-State": "0", "COM3-Parity": "None", "405 AO-Volts": "0.0000", "PRIME-TransposeMirrorY": "0", "PRIME-ChipName": "GS144BSI", "470 AO-Volts": "0.0000", "PRIME-TransposeMirrorX": "0", "445 Shutter-SupportsTriggering": "0", "470 Shutter-SupportsTriggering": "0", "XLIGHT Hub-Description": "XLIGHT hub", "PRIME-PreampOffLimit": "10000", "470 AO-Sequenceable": "1", "528 Shutter-MaxVolts": "5.0000", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "Core-XYStage": "XYStage", "470 AO-TriggerSampleRate": "100000", "PRIME-Timing-PreTriggerDelayNs": "24672000.0000", "528-DA Device": "528 Shutter", "Focus-Description": "Nikon TE2000 focus adapter", "FilterBlock-State": "5", "520 AO-Demo": "No", "COM3-DelayBetweenCharsMs": "0.0000", "470 Shutter-Volts": "0.0000", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "PRIME-PP 2 THRESHOLD": "125", "PRIME-PP 4 THRESHOLD": "75", "XYStage-Acceleration": "4.0000", "470 Shutter-Demo": "No", "PRIME-Name": "PMPCIECam00", "640-DA Device": "640 Shutter", "405 AO-Block voltage": "false", "ElapsedTime-ms": 385242, "PRIME-Offset": "100", "445 AO-IOChannel": "Dev1/ao1", "640 AO-MaxVolts": "5.0000", "PositionIndex": 9, "PRIME-PP 1 ENABLED": "Yes", "COM1-BaudRate": "9600", "640-State": "0", "COM3-Verbose": "1", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "LudlController-TransmissionDelay": "0", "PRIME-FullWellCapacity": "62000", "PRIME-SMARTStreamingEnabled": "No", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "640 Shutter-Description": "NI DAC", "528-Description": "DA device that is used as a shutter", "COM4-Description": "Serial port driver (boost:asio)", "405 Shutter-Description": "NI DAC", "TE2000-Name": "TE2000", "528 Shutter-Description": "NI DAC", "COM9-Description": "Serial port driver (boost:asio)", "LudlController-Name": "LudlController", "445 Shutter-TriggerSequenceLength": "1000", "PRIME-SerialNumber": "A18B203008", "445 AO-Volts": "0.0000", "PRIME-X-dimension": "1200", "PRIME-ShutterCloseDelay": "0", "XLIGHT Disk slider-State": "2", "Position": "Default", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 AO-IOChannel": "Dev1/ao5", "520 AO-IOChannel": "Dev1/ao4", "528 AO-TriggerSequenceLength": "1000", "405 Shutter-MaxVolts": "5.0000", "470-DA Device": "470 Shutter", "PRIME-FTCapable": "No", "Core-ChannelGroup": "Channels", "555 AO-SupportsTriggering": "0", "PRIME-Y-dimension": "1200", "Frame": 0, "555 Shutter-Sequenceable": "1", "PRIME-Timing-ExposureTimeNs": "499998000.0000", "Core-ImageProcessor": "", "528-State": "0", "520 AO-Block voltage": "false", "XLIGHT Excitation Wheel-Label": "QUAD", "640 Shutter-Demo": "No", "COM3-StopBits": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "520 Shutter-IOChannel": "Dev1/ao11", "Width": 1200, "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-SupportsTriggering": "0", "640 AO-TriggerSampleRate": "100000", "520-Description": "DA device that is used as a shutter", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "405 Shutter-Sequenceable": "1", "405 Shutter-SupportsTriggering": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-TriggerSampleRate": "100000", "640 Shutter-MaxVolts": "5.0000", "555 AO-MaxVolts": "5.0000", "520 AO-TriggerSequenceLength": "1000", "COM3-BaudRate": "9600", "COM9-BaudRate": "9600", "Vincent-D3-Channel #": "Ch. #1", "PRIME-PMode": "Normal", "OpticalPath-Label": "Left(100)", "BitDepth": 16, "555 AO-TriggerSequenceLength": "1000", "445 Shutter-Description": "NI DAC", "405 AO-Sequenceable": "1", "PRIME-AcquisitionMethod": "Callbacks", "555 AO-Name": "AnalogIO", "PRIME-ShutterMode": "Pre-Exposure", "COM3-Handshaking": "Off", "445 AO-SupportsTriggering": "0", "ROI": "0-0-1200-1200", "COM9-Verbose": "1", "PRIME-Color": "OFF", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "PRIME-ShutterOpenDelay": "0", "COM1-Parity": "None", "Slice": 9, "SlicePosition": 40, "COM9-DelayBetweenCharsMs": "0.0000", "555-Name": "DA Shutter", "PIEZO-LudlSingleAxisName": "B", "XLIGHT Emission Wheel-Label": "585/11(T)", "640 Shutter-Name": "AnalogIO", "640 AO-Sequenceable": "1", "520 Shutter-Demo": "No", "528 Shutter-Demo": "No", "470-Name": "DA Shutter", "520-DA Device": "520 Shutter", "XYStage-Description": "Ludl XY stage driver adapter", "445 Shutter-MinVolts": "0.0000", "528 AO-TriggerSampleRate": "100000", "405 AO-MinVolts": "0.0000", "405 Shutter-TriggerSequenceLength": "1000", "Height": 1200, "555 AO-Block voltage": "false", "640 Shutter-Volts": "0.0000", "PixelType": "GRAY16", "445 Shutter-Block voltage": "false", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "520 Shutter-TriggerSequenceLength": "1000", "COM9-Name": "COM9", "Core-SLM": "", "555 AO-Volts": "4.5000", "555 Shutter-Block voltage": "false", "XYStage-Speed": "1800.0000", "520 AO-MaxVolts": "5.0000", "528 Shutter-SupportsTriggering": "0", "COM1-Description": "Serial port driver (boost:asio)", "PRIME-MetadataEnabled": "No", "520 Shutter-MinVolts": "0.0000", "470 Shutter-Sequenceable": "1", "PRIME-PreampDelay": "0", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-MinVolts": "0.0000", "ChannelIndex": 1, "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Core-AutoShutter": "1", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "Exposure-ms": 160, "PRIME-PP 2 ENABLED": "Yes", "640 Shutter-TriggerSampleRate": "100000", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "470 Shutter-IOChannel": "Dev1/ao10", "405 AO-Demo": "No", "PixelSizeUm": 1.1, "470 AO-Demo": "No", "470 Shutter-MaxVolts": "5.0000", "Vincent-D3-Name": "Vincent-D3", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FileName": "img_000000000_Il-T_009.tif", "LudlController-Reset": "Operate", "PRIME-Exposure": "160.00", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "445-Name": "DA Shutter", "555 Shutter-Name": "AnalogIO", "520 Shutter-TriggerSampleRate": "100000", "445 Shutter-IOChannel": "Dev1/ao9", "PRIME-TransposeCorrection": "0", "XYStage-StepSize": "0.1000", "445-Description": "DA device that is used as a shutter", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 AO-IOChannel": "Dev1/ao7", "520 Shutter-Name": "AnalogIO", "COM3-DataBits": "8", "640 AO-Volts": "0.0000", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "555 Shutter-IOChannel": "Dev1/ao13", "WaitInterval": null, "520 Shutter-Sequenceable": "1", "YPositionUm": 31506.2, "445-State": "0", "445-DA Device": "445 Shutter", "PRIME-CircularBufferAutoSize": "ON", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "555 AO-TriggerSampleRate": "100000", "445 Shutter-Volts": "0.0000", "XLIGHT Hub-Port": "COM3", "Core-AutoFocus": "", "COM4-Handshaking": "Off", "528 Shutter-MinVolts": "0.0000", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "445 Shutter-Name": "AnalogIO", "528 Shutter-Sequenceable": "1", "XYStage-TransposeMirrorX": "0", "XYStage-TransposeMirrorY": "0", "PRIME-ReadoutRate": "100MHz 16bit", "XYStage-Name": "Stage", "640 AO-SupportsTriggering": "0", "640-Name": "DA Shutter", "640 Shutter-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "640 AO-Block voltage": "false", "445 Shutter-Sequenceable": "1", "470 AO-MaxVolts": "5.0000", "COM4-BaudRate": "9600", "470-Description": "DA device that is used as a shutter", "520 Shutter-Description": "NI DAC", "PRIME-CCDTemperature": "-14.8500", "Time": "2019-12-18 21:36:53 -0500", "640 AO-TriggerSequenceLength": "1000", "470 AO-Name": "AnalogIO", "XLIGHT Disk slider-Label": "Disk Pos 40um", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "Binning": "1x1", "TE2000-ModelType": "1", "COM3-AnswerTimeout": "500.0000", "555 Shutter-SupportsTriggering": "0", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "TE2000-Port": "COM4", "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Description": "NI DAC", "COM4-AnswerTimeout": "500.0000", "COM1-DelayBetweenCharsMs": "0.0000", "FilterBlock-Label": "Confocal", "445 AO-Name": "AnalogIO", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-Timing-ReadoutTimeNs": "24672000.0000", "528 AO-Demo": "No", "520 AO-Sequenceable": "1", "555-Description": "DA device that is used as a shutter", "405-DA Device": "405 Shutter", "405 AO-TriggerSequenceLength": "1000", "640 AO-MinVolts": "0.0000", "640 Shutter-Block voltage": "false", "FrameIndex": 0, "PRIME-Port": "CMOS", "555 AO-MinVolts": "0.0000", "COM3-Description": "Serial port driver (boost:asio)", "528 AO-Description": "NI DAC", "555 AO-IOChannel": "Dev1/ao6", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "555 AO-TriggerInputLine": "/Dev1/PFI0", "PRIME-ActualInterval-ms": "390.5200", "LudlController-Port": "COM9", "445 Shutter-Demo": "No", "PRIME-Trigger-Expose Out-Mux": "1", "SliceIndex": 9, "PRIME-CCDTemperatureSetPoint": "-15.0000", "405 Shutter-MinVolts": "0.0000", "PRIME-ExposeOutMode": "First Row", "AxisPositions": null, "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Volts": "5.0000", "528-Name": "DA Shutter", "555-State": "1", "XLIGHT Dichroic-Label": "QUAD", "445 AO-Sequenceable": "1", "405-Name": "DA Shutter", "405 AO-IOChannel": "Dev1/ao0", "Core-Camera": "PRIME", "Focus-Name": "Focus", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "405 Shutter-IOChannel": "Dev1/ao8", "640 AO-Demo": "No", "640 Shutter-SupportsTriggering": "0", "445 AO-Demo": "No", "555-DA Device": "555 Shutter", "470 Shutter-Description": "NI DAC", "528 AO-Volts": "0.0000", "COM1-DataBits": "8", "Vincent-D3-Address": "x", "520 AO-SupportsTriggering": "0", "405-Description": "DA device that is used as a shutter", "640 Shutter-MinVolts": "0.0000", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "405 AO-Name": "AnalogIO", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "640 AO-TriggerInputLine": "/Dev1/PFI0", "405 AO-TriggerInputLine": "/Dev1/PFI0", "640-Description": "DA device that is used as a shutter", "445 Shutter-TriggerSampleRate": "100000", "470 Shutter-TriggerSequenceLength": "1000", "COM1-StopBits": "1", "520 Shutter-SupportsTriggering": "0", "405-State": "0", "Core-Initialize": "1", "XLIGHT Emission Wheel-State": "3", "520 AO-Description": "NI DAC", "528 AO-TriggerInputLine": "/Dev1/PFI0", "445 Shutter-MaxVolts": "5.0000", "COM9-Handshaking": "Off", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "XLIGHT Spin motor-Label": "On (spinning)", "PRIME-PP 5 ENABLED": "No", "UUID": "25c410f1-1851-41d7-adf6-11713e66f0b8", "PRIME-TriggerMode": "Internal Trigger", "COM1-Handshaking": "Off", "PRIME-Timing-PostTriggerDelayNs": "49344000.0000", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "Focus-Position": "17741.5000", "528 Shutter-Block voltage": "false", "Channel": "Il-T", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "555 Shutter-Demo": "No", "405 Shutter-Demo": "No", "528 AO-Name": "AnalogIO", "520-State": "0", "Core-Galvo": "", "405 Shutter-TriggerSampleRate": "100000", "Focus-FocusStepSize_(nm)": "100", "PRIME-PixelType": "16bit", "COM4-DataBits": "8", "470 AO-IOChannel": "Dev1/ao2", "520 AO-MinVolts": "0.0000", "COM9-Parity": "None", "PRIME-CircularBufferEnabled": "ON", "PRIME-PP 6 Reset": "No", "640 AO-Description": "NI DAC", "Source": "PRIME", "445 AO-MinVolts": "0.0000", "PIEZO-Name": "Stage", "445 AO-Description": "NI DAC", "PRIME-CircularBufferFrameCount": "46", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "520 AO-Volts": "0.0000", "528 Shutter-TriggerSampleRate": "100000", "XPositionUm": 19731.9, "OpticalPath-State": "4", "PRIME-PP 3 THRESHOLD": "80", "PRIME-PVCAM Version": "3.7.1", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Gain": "1-HDR", "640 Shutter-TriggerSequenceLength": "1000", "528 AO-SupportsTriggering": "0", "445 AO-TriggerInputLine": "/Dev1/PFI0", "OpticalPath-Name": "OpticalPath", "PRIME-PP 4 ENABLED": "Yes", "PRIME-PVCAM Adapter Version": "1.3.29", "COM3-Name": "COM3", "405 AO-MaxVolts": "5.0000", "520 Shutter-Block voltage": "false", "PRIME-Timing-ClearingTimeNs": "49344000.0000", "Core-Focus": "PIEZO", "528 AO-Block voltage": "false", "TE2000-Description": "Nikon TE2000 hub", "528 AO-MinVolts": "0.0000", "COM4-Parity": "None", "Summary": { "Slices": 11, "ChContrastMin": [ 0, 0, 0, 0, 0, 0 ], "PixelAspect": 1, "SlicesFirst": true, "Interval_ms": 0, "Time": "2019-12-18 21:36:42 -0500", "Prefix": "seq_4", "InitialPositionList": [ { "Label": "Pos0", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.21], "Focus": [17741.5], "XYStage": [ 44542.200000000004, 31636.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos1", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.22], "Focus": [17741.4], "XYStage": [ 43801.100000000006, 31637.9 ] }, "GridColumnIndex": 0 }, { "Label": "Pos2", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.5], "XYStage": [ 38561.3, 32040.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos3", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.185], "Focus": [17741.3], "XYStage": [ 37698.4, 32041.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos4", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.16], "Focus": [17741.5], "XYStage": [ 32375.4, 31758.9 ] }, "GridColumnIndex": 0 }, { "Label": "Pos5", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.17000000000002], "Focus": [17741.3], "XYStage": [ 31770.7, 31758.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos6", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.4], "XYStage": [ 26536.800000000003, 31068.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos7", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.205], "Focus": [17741.4], "XYStage": [ 25420.2, 31068.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos8", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.85], "Focus": [17741.3], "XYStage": [ 21059, 31508 ] }, "GridColumnIndex": 0 }, { "Label": "Pos9", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.82], "Focus": [17741.3], "XYStage": [ 19731.9, 31506.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos10", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.79], "Focus": [17741.4], "XYStage": [ 14528.5, 32007.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos11", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.83], "Focus": [17741.2], "XYStage": [ 13830.300000000001, 32009.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos12", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.79], "Focus": [17741.3], "XYStage": [ 8775.300000000001, 32017.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos13", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.815], "Focus": [17741.3], "XYStage": [ 8109.200000000001, 32008.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos14", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.805], "Focus": [17741.3], "XYStage": [ 3483.3, 31908.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos15", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.82], "Focus": [17741.2], "XYStage": [ 2686.8, 31903.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos16", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [211.48000000000002], "Focus": [17741.1], "XYStage": [ -1978.9, 31973.600000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos17", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [211.51500000000001], "Focus": [17741.1], "XYStage": [ -2957.8, 31982.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos18", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.195], "Focus": [17741.5], "XYStage": [ 47747.4, -576.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos19", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.23000000000002], "Focus": [17741.6], "XYStage": [ 47053.9, -576.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos20", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.205], "Focus": [17741.4], "XYStage": [ 41871.200000000004, -479.8 ] }, "GridColumnIndex": 0 }, { "Label": "Pos21", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.4], "XYStage": [ 40882.600000000006, -392 ] }, "GridColumnIndex": 0 }, { "Label": "Pos22", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.24], "Focus": [17741.4], "XYStage": [ 35905.6, -135.3 ] }, "GridColumnIndex": 0 }, { "Label": "Pos23", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.195], "Focus": [17741.5], "XYStage": [ 34990.9, -132.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos24", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.46], "Focus": [17741.4], "XYStage": [ 29678.100000000002, -958.1 ] }, "GridColumnIndex": 0 }, { "Label": "Pos25", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.44], "Focus": [17741.5], "XYStage": [ 28983.600000000002, -939.4000000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos26", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.475], "Focus": [17741.5], "XYStage": [ 23019.7, -122 ] }, "GridColumnIndex": 0 }, { "Label": "Pos27", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.44], "Focus": [17741.7], "XYStage": [ 22428, -116.80000000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos28", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.46], "Focus": [17741.7], "XYStage": [ 16364.900000000001, -181.10000000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos29", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.48000000000002], "Focus": [17741.5], "XYStage": [ 15712.1, -180 ] }, "GridColumnIndex": 0 }, { "Label": "Pos30", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.45000000000002], "Focus": [17741.7], "XYStage": [ 10346.1, 77.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos31", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.49], "Focus": [17741.4], "XYStage": [ 9755.5, 85.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos32", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.475], "Focus": [17741.5], "XYStage": [ 4775.5, 293.90000000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos33", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.445], "Focus": [17741.4], "XYStage": [ 3954.9, 293.90000000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos34", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.455], "Focus": [17741.4], "XYStage": [ -1324.9, 545.6 ] }, "GridColumnIndex": 0 }, { "Label": "Pos35", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.445], "Focus": [17741.3], "XYStage": [ -2097, 576.6 ] }, "GridColumnIndex": 0 } ], "z-step_um": 10, "GridRow": 0, "BitDepth": 16, "Depth": 2, "Channels": 6, "Source": "Micro-Manager", "ChColors": [ -16711681, -256, -65281, -1, -1, -16776961 ], "MetadataVersion": 10, "KeepShutterOpenChannels": true, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536, 65536 ], "CustomIntervals_ms": [], "Height": 1200, "MicroManagerVersion": "1.4.23 20180219", "GridColumn": 0, "UUID": "14a13bf7-c944-4672-b873-fcfbbb7ea13d", "PixelType": "GRAY16", "Width": 1200, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\Yan\\20191218-YC113-S7S8-miseq", "Frames": 1, "Comment": "", "UserName": "Nikon", "PixelSize_um": 1.1, "ROI": [ 0, 0, 1200, 1200 ], "Date": "2019-12-18", "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC", "P-DAPI-high" ], "KeepShutterOpenSlices": true, "IJType": 1, "ComputerName": "ZadorConfocal2", "Positions": 36, "PositionIndex": 9, "CameraTimeout": 20000, "TimeFirst": false }, "640 AO-Name": "AnalogIO", "Vincent-D3-Command": "Close" }, "FrameKey-0-1-10": { "COM4-StopBits": "1", "528 AO-MaxVolts": "5.0000", "XLIGHT Excitation Wheel-State": "1", "470 Shutter-Name": "AnalogIO", "Core-Shutter": "555", "555 AO-Demo": "No", "XLIGHT Dichroic-State": "2", "XYStage-StepSize-Y": "0.1000", "ZPositionUm": 298.82, "XYStage-StepSize-X": "0.1000", "PRIME-FirmwareVersion": "20.28", "PRIME-ClearCycles": "2", "520-Name": "DA Shutter", "405 Shutter-Name": "AnalogIO", "PIEZO-StepSize": "0.0050", "PRIME-ClearMode": "Pre-Exposure", "470 AO-MinVolts": "0.0000", "405 AO-SupportsTriggering": "1", "CameraChannelIndex": 0, "XYStage-StartSpeed": "500.0000", "COM9-StopBits": "2", "405 AO-Description": "NI DAC", "COM4-DelayBetweenCharsMs": "0.0000", "445 AO-Block voltage": "false", "520 AO-TriggerInputLine": "/Dev1/PFI0", "555 AO-Description": "NI DAC", "520 AO-TriggerSampleRate": "100000", "COM4-Verbose": "1", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM4-Name": "COM4", "405 Shutter-Volts": "0.0000", "Vincent-D3-Port": "COM1", "PRIME-PP 4 MIN ADU AFFECTED": "200", "470 Shutter-MinVolts": "0.0000", "PRIME-PP 3 ENABLED": "Yes", "470 AO-Description": "NI DAC", "PRIME-Description": "PVCAM API device adapter", "520 Shutter-MaxVolts": "5.0000", "520 Shutter-Volts": "0.0000", "528 AO-Sequenceable": "1", "PRIME-Binning": "1x1", "555 Shutter-MaxVolts": "5.0000", "528 Shutter-TriggerSequenceLength": "1000", "405 Shutter-Block voltage": "false", "XLIGHT Spin motor-State": "1", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "Vincent-D3-Time to open (ms)": "35.0000", "Vincent-D3-Time to close (ms)": "35.0000", "445 AO-TriggerSampleRate": "100000", "640 Shutter-IOChannel": "Dev1/ao14", "555 AO-Sequenceable": "1", "520 AO-Name": "AnalogIO", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "470 AO-Block voltage": "false", "COM1-Verbose": "1", "COM9-AnswerTimeout": "10000.0000", "528 Shutter-Name": "AnalogIO", "PRIME-Trigger Timeout (secs)": "10", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "NextFrame": 0, "470 AO-TriggerSequenceLength": "1000", "PRIME-TransposeXY": "0", "445 AO-TriggerSequenceLength": "1000", "COM1-Name": "COM1", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "COM9-DataBits": "8", "COM1-AnswerTimeout": "500.0000", "Camera": "", "TE2000-Version": "V2.50-V2.50-V2.10", "528 Shutter-Volts": "0.0000", "XLIGHT Hub-Name": "XLIGHT Hub", "PIEZO-Description": "Ludl stage driver adapter", "PositionName": "Pos9", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSampleRate": "100000", "PRIME-FanSpeedSetpoint": "High", "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "405 AO-TriggerSampleRate": "100000", "470 Shutter-Block voltage": "false", "470-State": "0", "COM3-Parity": "None", "405 AO-Volts": "0.0000", "PRIME-TransposeMirrorY": "0", "PRIME-ChipName": "GS144BSI", "470 AO-Volts": "0.0000", "PRIME-TransposeMirrorX": "0", "445 Shutter-SupportsTriggering": "0", "470 Shutter-SupportsTriggering": "0", "XLIGHT Hub-Description": "XLIGHT hub", "PRIME-PreampOffLimit": "10000", "470 AO-Sequenceable": "1", "528 Shutter-MaxVolts": "5.0000", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "Core-XYStage": "XYStage", "470 AO-TriggerSampleRate": "100000", "PRIME-Timing-PreTriggerDelayNs": "24672000.0000", "528-DA Device": "528 Shutter", "Focus-Description": "Nikon TE2000 focus adapter", "FilterBlock-State": "5", "520 AO-Demo": "No", "COM3-DelayBetweenCharsMs": "0.0000", "470 Shutter-Volts": "0.0000", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "PRIME-PP 2 THRESHOLD": "125", "PRIME-PP 4 THRESHOLD": "75", "XYStage-Acceleration": "4.0000", "470 Shutter-Demo": "No", "PRIME-Name": "PMPCIECam00", "640-DA Device": "640 Shutter", "405 AO-Block voltage": "false", "ElapsedTime-ms": 385692, "PRIME-Offset": "100", "445 AO-IOChannel": "Dev1/ao1", "640 AO-MaxVolts": "5.0000", "PositionIndex": 9, "PRIME-PP 1 ENABLED": "Yes", "COM1-BaudRate": "9600", "640-State": "0", "COM3-Verbose": "1", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "LudlController-TransmissionDelay": "0", "PRIME-FullWellCapacity": "62000", "PRIME-SMARTStreamingEnabled": "No", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "640 Shutter-Description": "NI DAC", "528-Description": "DA device that is used as a shutter", "COM4-Description": "Serial port driver (boost:asio)", "405 Shutter-Description": "NI DAC", "TE2000-Name": "TE2000", "528 Shutter-Description": "NI DAC", "COM9-Description": "Serial port driver (boost:asio)", "LudlController-Name": "LudlController", "445 Shutter-TriggerSequenceLength": "1000", "PRIME-SerialNumber": "A18B203008", "445 AO-Volts": "0.0000", "PRIME-X-dimension": "1200", "PRIME-ShutterCloseDelay": "0", "XLIGHT Disk slider-State": "2", "Position": "Default", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 AO-IOChannel": "Dev1/ao5", "520 AO-IOChannel": "Dev1/ao4", "528 AO-TriggerSequenceLength": "1000", "405 Shutter-MaxVolts": "5.0000", "470-DA Device": "470 Shutter", "PRIME-FTCapable": "No", "Core-ChannelGroup": "Channels", "555 AO-SupportsTriggering": "0", "PRIME-Y-dimension": "1200", "Frame": 0, "555 Shutter-Sequenceable": "1", "PRIME-Timing-ExposureTimeNs": "499998000.0000", "Core-ImageProcessor": "", "528-State": "0", "520 AO-Block voltage": "false", "XLIGHT Excitation Wheel-Label": "QUAD", "640 Shutter-Demo": "No", "COM3-StopBits": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "520 Shutter-IOChannel": "Dev1/ao11", "Width": 1200, "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-SupportsTriggering": "0", "640 AO-TriggerSampleRate": "100000", "520-Description": "DA device that is used as a shutter", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "405 Shutter-Sequenceable": "1", "405 Shutter-SupportsTriggering": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-TriggerSampleRate": "100000", "640 Shutter-MaxVolts": "5.0000", "555 AO-MaxVolts": "5.0000", "520 AO-TriggerSequenceLength": "1000", "COM3-BaudRate": "9600", "COM9-BaudRate": "9600", "Vincent-D3-Channel #": "Ch. #1", "PRIME-PMode": "Normal", "OpticalPath-Label": "Left(100)", "BitDepth": 16, "555 AO-TriggerSequenceLength": "1000", "445 Shutter-Description": "NI DAC", "405 AO-Sequenceable": "1", "PRIME-AcquisitionMethod": "Callbacks", "555 AO-Name": "AnalogIO", "PRIME-ShutterMode": "Pre-Exposure", "COM3-Handshaking": "Off", "445 AO-SupportsTriggering": "0", "ROI": "0-0-1200-1200", "COM9-Verbose": "1", "PRIME-Color": "OFF", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "PRIME-ShutterOpenDelay": "0", "COM1-Parity": "None", "Slice": 10, "SlicePosition": 50, "COM9-DelayBetweenCharsMs": "0.0000", "555-Name": "DA Shutter", "PIEZO-LudlSingleAxisName": "B", "XLIGHT Emission Wheel-Label": "585/11(T)", "640 Shutter-Name": "AnalogIO", "640 AO-Sequenceable": "1", "520 Shutter-Demo": "No", "528 Shutter-Demo": "No", "470-Name": "DA Shutter", "520-DA Device": "520 Shutter", "XYStage-Description": "Ludl XY stage driver adapter", "445 Shutter-MinVolts": "0.0000", "528 AO-TriggerSampleRate": "100000", "405 AO-MinVolts": "0.0000", "405 Shutter-TriggerSequenceLength": "1000", "Height": 1200, "555 AO-Block voltage": "false", "640 Shutter-Volts": "0.0000", "PixelType": "GRAY16", "445 Shutter-Block voltage": "false", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "520 Shutter-TriggerSequenceLength": "1000", "COM9-Name": "COM9", "Core-SLM": "", "555 AO-Volts": "4.5000", "555 Shutter-Block voltage": "false", "XYStage-Speed": "1800.0000", "520 AO-MaxVolts": "5.0000", "528 Shutter-SupportsTriggering": "0", "COM1-Description": "Serial port driver (boost:asio)", "PRIME-MetadataEnabled": "No", "520 Shutter-MinVolts": "0.0000", "470 Shutter-Sequenceable": "1", "PRIME-PreampDelay": "0", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-MinVolts": "0.0000", "ChannelIndex": 1, "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Core-AutoShutter": "1", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "Exposure-ms": 160, "PRIME-PP 2 ENABLED": "Yes", "640 Shutter-TriggerSampleRate": "100000", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "470 Shutter-IOChannel": "Dev1/ao10", "405 AO-Demo": "No", "PixelSizeUm": 1.1, "470 AO-Demo": "No", "470 Shutter-MaxVolts": "5.0000", "Vincent-D3-Name": "Vincent-D3", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FileName": "img_000000000_Il-T_010.tif", "LudlController-Reset": "Operate", "PRIME-Exposure": "160.00", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "445-Name": "DA Shutter", "555 Shutter-Name": "AnalogIO", "520 Shutter-TriggerSampleRate": "100000", "445 Shutter-IOChannel": "Dev1/ao9", "PRIME-TransposeCorrection": "0", "XYStage-StepSize": "0.1000", "445-Description": "DA device that is used as a shutter", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 AO-IOChannel": "Dev1/ao7", "520 Shutter-Name": "AnalogIO", "COM3-DataBits": "8", "640 AO-Volts": "0.0000", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "555 Shutter-IOChannel": "Dev1/ao13", "WaitInterval": null, "520 Shutter-Sequenceable": "1", "YPositionUm": 31506.2, "445-State": "0", "445-DA Device": "445 Shutter", "PRIME-CircularBufferAutoSize": "ON", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "555 AO-TriggerSampleRate": "100000", "445 Shutter-Volts": "0.0000", "XLIGHT Hub-Port": "COM3", "Core-AutoFocus": "", "COM4-Handshaking": "Off", "528 Shutter-MinVolts": "0.0000", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "445 Shutter-Name": "AnalogIO", "528 Shutter-Sequenceable": "1", "XYStage-TransposeMirrorX": "0", "XYStage-TransposeMirrorY": "0", "PRIME-ReadoutRate": "100MHz 16bit", "XYStage-Name": "Stage", "640 AO-SupportsTriggering": "0", "640-Name": "DA Shutter", "640 Shutter-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "640 AO-Block voltage": "false", "445 Shutter-Sequenceable": "1", "470 AO-MaxVolts": "5.0000", "COM4-BaudRate": "9600", "470-Description": "DA device that is used as a shutter", "520 Shutter-Description": "NI DAC", "PRIME-CCDTemperature": "-14.8500", "Time": "2019-12-18 21:36:53 -0500", "640 AO-TriggerSequenceLength": "1000", "470 AO-Name": "AnalogIO", "XLIGHT Disk slider-Label": "Disk Pos 40um", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "Binning": "1x1", "TE2000-ModelType": "1", "COM3-AnswerTimeout": "500.0000", "555 Shutter-SupportsTriggering": "0", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "TE2000-Port": "COM4", "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Description": "NI DAC", "COM4-AnswerTimeout": "500.0000", "COM1-DelayBetweenCharsMs": "0.0000", "FilterBlock-Label": "Confocal", "445 AO-Name": "AnalogIO", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-Timing-ReadoutTimeNs": "24672000.0000", "528 AO-Demo": "No", "520 AO-Sequenceable": "1", "555-Description": "DA device that is used as a shutter", "405-DA Device": "405 Shutter", "405 AO-TriggerSequenceLength": "1000", "640 AO-MinVolts": "0.0000", "640 Shutter-Block voltage": "false", "FrameIndex": 0, "PRIME-Port": "CMOS", "555 AO-MinVolts": "0.0000", "COM3-Description": "Serial port driver (boost:asio)", "528 AO-Description": "NI DAC", "555 AO-IOChannel": "Dev1/ao6", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "555 AO-TriggerInputLine": "/Dev1/PFI0", "PRIME-ActualInterval-ms": "390.5200", "LudlController-Port": "COM9", "445 Shutter-Demo": "No", "PRIME-Trigger-Expose Out-Mux": "1", "SliceIndex": 10, "PRIME-CCDTemperatureSetPoint": "-15.0000", "405 Shutter-MinVolts": "0.0000", "PRIME-ExposeOutMode": "First Row", "AxisPositions": null, "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Volts": "5.0000", "528-Name": "DA Shutter", "555-State": "0", "XLIGHT Dichroic-Label": "QUAD", "445 AO-Sequenceable": "1", "405-Name": "DA Shutter", "405 AO-IOChannel": "Dev1/ao0", "Core-Camera": "PRIME", "Focus-Name": "Focus", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "405 Shutter-IOChannel": "Dev1/ao8", "640 AO-Demo": "No", "640 Shutter-SupportsTriggering": "0", "445 AO-Demo": "No", "555-DA Device": "555 Shutter", "470 Shutter-Description": "NI DAC", "528 AO-Volts": "0.0000", "COM1-DataBits": "8", "Vincent-D3-Address": "x", "520 AO-SupportsTriggering": "0", "405-Description": "DA device that is used as a shutter", "640 Shutter-MinVolts": "0.0000", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "405 AO-Name": "AnalogIO", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "640 AO-TriggerInputLine": "/Dev1/PFI0", "405 AO-TriggerInputLine": "/Dev1/PFI0", "640-Description": "DA device that is used as a shutter", "445 Shutter-TriggerSampleRate": "100000", "470 Shutter-TriggerSequenceLength": "1000", "COM1-StopBits": "1", "520 Shutter-SupportsTriggering": "0", "405-State": "0", "Core-Initialize": "1", "XLIGHT Emission Wheel-State": "3", "520 AO-Description": "NI DAC", "528 AO-TriggerInputLine": "/Dev1/PFI0", "445 Shutter-MaxVolts": "5.0000", "COM9-Handshaking": "Off", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "XLIGHT Spin motor-Label": "On (spinning)", "PRIME-PP 5 ENABLED": "No", "UUID": "841bd1cb-564d-4ea7-81c0-aa3bdb1d99a9", "PRIME-TriggerMode": "Internal Trigger", "COM1-Handshaking": "Off", "PRIME-Timing-PostTriggerDelayNs": "49344000.0000", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "Focus-Position": "17741.5000", "528 Shutter-Block voltage": "false", "Channel": "Il-T", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "555 Shutter-Demo": "No", "405 Shutter-Demo": "No", "528 AO-Name": "AnalogIO", "520-State": "0", "Core-Galvo": "", "405 Shutter-TriggerSampleRate": "100000", "Focus-FocusStepSize_(nm)": "100", "PRIME-PixelType": "16bit", "COM4-DataBits": "8", "470 AO-IOChannel": "Dev1/ao2", "520 AO-MinVolts": "0.0000", "COM9-Parity": "None", "PRIME-CircularBufferEnabled": "ON", "PRIME-PP 6 Reset": "No", "640 AO-Description": "NI DAC", "Source": "PRIME", "445 AO-MinVolts": "0.0000", "PIEZO-Name": "Stage", "445 AO-Description": "NI DAC", "PRIME-CircularBufferFrameCount": "46", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "520 AO-Volts": "0.0000", "528 Shutter-TriggerSampleRate": "100000", "XPositionUm": 19731.9, "OpticalPath-State": "4", "PRIME-PP 3 THRESHOLD": "80", "PRIME-PVCAM Version": "3.7.1", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Gain": "1-HDR", "640 Shutter-TriggerSequenceLength": "1000", "528 AO-SupportsTriggering": "0", "445 AO-TriggerInputLine": "/Dev1/PFI0", "OpticalPath-Name": "OpticalPath", "PRIME-PP 4 ENABLED": "Yes", "PRIME-PVCAM Adapter Version": "1.3.29", "COM3-Name": "COM3", "405 AO-MaxVolts": "5.0000", "520 Shutter-Block voltage": "false", "PRIME-Timing-ClearingTimeNs": "49344000.0000", "Core-Focus": "PIEZO", "528 AO-Block voltage": "false", "TE2000-Description": "Nikon TE2000 hub", "528 AO-MinVolts": "0.0000", "COM4-Parity": "None", "Summary": { "Slices": 11, "ChContrastMin": [ 0, 0, 0, 0, 0, 0 ], "PixelAspect": 1, "SlicesFirst": true, "Interval_ms": 0, "Time": "2019-12-18 21:36:42 -0500", "Prefix": "seq_4", "InitialPositionList": [ { "Label": "Pos0", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.21], "Focus": [17741.5], "XYStage": [ 44542.200000000004, 31636.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos1", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.22], "Focus": [17741.4], "XYStage": [ 43801.100000000006, 31637.9 ] }, "GridColumnIndex": 0 }, { "Label": "Pos2", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.5], "XYStage": [ 38561.3, 32040.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos3", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.185], "Focus": [17741.3], "XYStage": [ 37698.4, 32041.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos4", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.16], "Focus": [17741.5], "XYStage": [ 32375.4, 31758.9 ] }, "GridColumnIndex": 0 }, { "Label": "Pos5", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.17000000000002], "Focus": [17741.3], "XYStage": [ 31770.7, 31758.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos6", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.4], "XYStage": [ 26536.800000000003, 31068.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos7", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.205], "Focus": [17741.4], "XYStage": [ 25420.2, 31068.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos8", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.85], "Focus": [17741.3], "XYStage": [ 21059, 31508 ] }, "GridColumnIndex": 0 }, { "Label": "Pos9", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.82], "Focus": [17741.3], "XYStage": [ 19731.9, 31506.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos10", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.79], "Focus": [17741.4], "XYStage": [ 14528.5, 32007.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos11", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.83], "Focus": [17741.2], "XYStage": [ 13830.300000000001, 32009.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos12", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.79], "Focus": [17741.3], "XYStage": [ 8775.300000000001, 32017.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos13", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.815], "Focus": [17741.3], "XYStage": [ 8109.200000000001, 32008.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos14", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.805], "Focus": [17741.3], "XYStage": [ 3483.3, 31908.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos15", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.82], "Focus": [17741.2], "XYStage": [ 2686.8, 31903.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos16", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [211.48000000000002], "Focus": [17741.1], "XYStage": [ -1978.9, 31973.600000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos17", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [211.51500000000001], "Focus": [17741.1], "XYStage": [ -2957.8, 31982.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos18", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.195], "Focus": [17741.5], "XYStage": [ 47747.4, -576.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos19", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.23000000000002], "Focus": [17741.6], "XYStage": [ 47053.9, -576.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos20", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.205], "Focus": [17741.4], "XYStage": [ 41871.200000000004, -479.8 ] }, "GridColumnIndex": 0 }, { "Label": "Pos21", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.4], "XYStage": [ 40882.600000000006, -392 ] }, "GridColumnIndex": 0 }, { "Label": "Pos22", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.24], "Focus": [17741.4], "XYStage": [ 35905.6, -135.3 ] }, "GridColumnIndex": 0 }, { "Label": "Pos23", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.195], "Focus": [17741.5], "XYStage": [ 34990.9, -132.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos24", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.46], "Focus": [17741.4], "XYStage": [ 29678.100000000002, -958.1 ] }, "GridColumnIndex": 0 }, { "Label": "Pos25", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.44], "Focus": [17741.5], "XYStage": [ 28983.600000000002, -939.4000000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos26", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.475], "Focus": [17741.5], "XYStage": [ 23019.7, -122 ] }, "GridColumnIndex": 0 }, { "Label": "Pos27", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.44], "Focus": [17741.7], "XYStage": [ 22428, -116.80000000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos28", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.46], "Focus": [17741.7], "XYStage": [ 16364.900000000001, -181.10000000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos29", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.48000000000002], "Focus": [17741.5], "XYStage": [ 15712.1, -180 ] }, "GridColumnIndex": 0 }, { "Label": "Pos30", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.45000000000002], "Focus": [17741.7], "XYStage": [ 10346.1, 77.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos31", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.49], "Focus": [17741.4], "XYStage": [ 9755.5, 85.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos32", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.475], "Focus": [17741.5], "XYStage": [ 4775.5, 293.90000000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos33", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.445], "Focus": [17741.4], "XYStage": [ 3954.9, 293.90000000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos34", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.455], "Focus": [17741.4], "XYStage": [ -1324.9, 545.6 ] }, "GridColumnIndex": 0 }, { "Label": "Pos35", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.445], "Focus": [17741.3], "XYStage": [ -2097, 576.6 ] }, "GridColumnIndex": 0 } ], "z-step_um": 10, "GridRow": 0, "BitDepth": 16, "Depth": 2, "Channels": 6, "Source": "Micro-Manager", "ChColors": [ -16711681, -256, -65281, -1, -1, -16776961 ], "MetadataVersion": 10, "KeepShutterOpenChannels": true, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536, 65536 ], "CustomIntervals_ms": [], "Height": 1200, "MicroManagerVersion": "1.4.23 20180219", "GridColumn": 0, "UUID": "14a13bf7-c944-4672-b873-fcfbbb7ea13d", "PixelType": "GRAY16", "Width": 1200, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\Yan\\20191218-YC113-S7S8-miseq", "Frames": 1, "Comment": "", "UserName": "Nikon", "PixelSize_um": 1.1, "ROI": [ 0, 0, 1200, 1200 ], "Date": "2019-12-18", "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC", "P-DAPI-high" ], "KeepShutterOpenSlices": true, "IJType": 1, "ComputerName": "ZadorConfocal2", "Positions": 36, "PositionIndex": 9, "CameraTimeout": 20000, "TimeFirst": false }, "640 AO-Name": "AnalogIO", "Vincent-D3-Command": "Close" }, "FrameKey-0-2-0": { "COM4-StopBits": "1", "528 AO-MaxVolts": "5.0000", "XLIGHT Excitation Wheel-State": "1", "470 Shutter-Name": "AnalogIO", "Core-Shutter": "640", "555 AO-Demo": "No", "XLIGHT Dichroic-State": "2", "XYStage-StepSize-Y": "0.1000", "ZPositionUm": 198.82, "XYStage-StepSize-X": "0.1000", "PRIME-FirmwareVersion": "20.28", "PRIME-ClearCycles": "2", "520-Name": "DA Shutter", "405 Shutter-Name": "AnalogIO", "PIEZO-StepSize": "0.0050", "PRIME-ClearMode": "Pre-Exposure", "470 AO-MinVolts": "0.0000", "405 AO-SupportsTriggering": "1", "CameraChannelIndex": 0, "XYStage-StartSpeed": "500.0000", "COM9-StopBits": "2", "405 AO-Description": "NI DAC", "COM4-DelayBetweenCharsMs": "0.0000", "445 AO-Block voltage": "false", "520 AO-TriggerInputLine": "/Dev1/PFI0", "555 AO-Description": "NI DAC", "520 AO-TriggerSampleRate": "100000", "COM4-Verbose": "1", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM4-Name": "COM4", "405 Shutter-Volts": "0.0000", "Vincent-D3-Port": "COM1", "PRIME-PP 4 MIN ADU AFFECTED": "200", "470 Shutter-MinVolts": "0.0000", "PRIME-PP 3 ENABLED": "Yes", "470 AO-Description": "NI DAC", "PRIME-Description": "PVCAM API device adapter", "520 Shutter-MaxVolts": "5.0000", "520 Shutter-Volts": "0.0000", "528 AO-Sequenceable": "1", "PRIME-Binning": "1x1", "555 Shutter-MaxVolts": "5.0000", "528 Shutter-TriggerSequenceLength": "1000", "405 Shutter-Block voltage": "false", "XLIGHT Spin motor-State": "1", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "Vincent-D3-Time to open (ms)": "35.0000", "Vincent-D3-Time to close (ms)": "35.0000", "445 AO-TriggerSampleRate": "100000", "640 Shutter-IOChannel": "Dev1/ao14", "555 AO-Sequenceable": "1", "520 AO-Name": "AnalogIO", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "470 AO-Block voltage": "false", "COM1-Verbose": "1", "COM9-AnswerTimeout": "10000.0000", "528 Shutter-Name": "AnalogIO", "PRIME-Trigger Timeout (secs)": "10", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "NextFrame": 0, "470 AO-TriggerSequenceLength": "1000", "PRIME-TransposeXY": "0", "445 AO-TriggerSequenceLength": "1000", "COM1-Name": "COM1", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "COM9-DataBits": "8", "COM1-AnswerTimeout": "500.0000", "Camera": "", "TE2000-Version": "V2.50-V2.50-V2.10", "528 Shutter-Volts": "0.0000", "XLIGHT Hub-Name": "XLIGHT Hub", "PIEZO-Description": "Ludl stage driver adapter", "PositionName": "Pos9", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSampleRate": "100000", "PRIME-FanSpeedSetpoint": "High", "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "405 AO-TriggerSampleRate": "100000", "470 Shutter-Block voltage": "false", "470-State": "0", "COM3-Parity": "None", "405 AO-Volts": "0.0000", "PRIME-TransposeMirrorY": "0", "PRIME-ChipName": "GS144BSI", "470 AO-Volts": "0.0000", "PRIME-TransposeMirrorX": "0", "445 Shutter-SupportsTriggering": "0", "470 Shutter-SupportsTriggering": "0", "XLIGHT Hub-Description": "XLIGHT hub", "PRIME-PreampOffLimit": "10000", "470 AO-Sequenceable": "1", "528 Shutter-MaxVolts": "5.0000", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "Core-XYStage": "XYStage", "470 AO-TriggerSampleRate": "100000", "PRIME-Timing-PreTriggerDelayNs": "24672000.0000", "528-DA Device": "528 Shutter", "Focus-Description": "Nikon TE2000 focus adapter", "FilterBlock-State": "5", "520 AO-Demo": "No", "COM3-DelayBetweenCharsMs": "0.0000", "470 Shutter-Volts": "0.0000", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "PRIME-PP 2 THRESHOLD": "125", "PRIME-PP 4 THRESHOLD": "75", "XYStage-Acceleration": "4.0000", "470 Shutter-Demo": "No", "PRIME-Name": "PMPCIECam00", "640-DA Device": "640 Shutter", "405 AO-Block voltage": "false", "ElapsedTime-ms": 386814, "PRIME-Offset": "100", "445 AO-IOChannel": "Dev1/ao1", "640 AO-MaxVolts": "5.0000", "PositionIndex": 9, "PRIME-PP 1 ENABLED": "Yes", "COM1-BaudRate": "9600", "640-State": "1", "COM3-Verbose": "1", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "LudlController-TransmissionDelay": "0", "PRIME-FullWellCapacity": "62000", "PRIME-SMARTStreamingEnabled": "No", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "640 Shutter-Description": "NI DAC", "528-Description": "DA device that is used as a shutter", "COM4-Description": "Serial port driver (boost:asio)", "405 Shutter-Description": "NI DAC", "TE2000-Name": "TE2000", "528 Shutter-Description": "NI DAC", "COM9-Description": "Serial port driver (boost:asio)", "LudlController-Name": "LudlController", "445 Shutter-TriggerSequenceLength": "1000", "PRIME-SerialNumber": "A18B203008", "445 AO-Volts": "0.0000", "PRIME-X-dimension": "1200", "PRIME-ShutterCloseDelay": "0", "XLIGHT Disk slider-State": "2", "Position": "Default", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 AO-IOChannel": "Dev1/ao5", "520 AO-IOChannel": "Dev1/ao4", "528 AO-TriggerSequenceLength": "1000", "405 Shutter-MaxVolts": "5.0000", "470-DA Device": "470 Shutter", "PRIME-FTCapable": "No", "Core-ChannelGroup": "Channels", "555 AO-SupportsTriggering": "0", "PRIME-Y-dimension": "1200", "Frame": 0, "555 Shutter-Sequenceable": "1", "PRIME-Timing-ExposureTimeNs": "499998000.0000", "Core-ImageProcessor": "", "528-State": "0", "520 AO-Block voltage": "false", "XLIGHT Excitation Wheel-Label": "QUAD", "640 Shutter-Demo": "No", "COM3-StopBits": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "520 Shutter-IOChannel": "Dev1/ao11", "Width": 1200, "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-SupportsTriggering": "0", "640 AO-TriggerSampleRate": "100000", "520-Description": "DA device that is used as a shutter", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "405 Shutter-Sequenceable": "1", "405 Shutter-SupportsTriggering": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-TriggerSampleRate": "100000", "640 Shutter-MaxVolts": "5.0000", "555 AO-MaxVolts": "5.0000", "520 AO-TriggerSequenceLength": "1000", "COM3-BaudRate": "9600", "COM9-BaudRate": "9600", "Vincent-D3-Channel #": "Ch. #1", "PRIME-PMode": "Normal", "OpticalPath-Label": "Left(100)", "BitDepth": 16, "555 AO-TriggerSequenceLength": "1000", "445 Shutter-Description": "NI DAC", "405 AO-Sequenceable": "1", "PRIME-AcquisitionMethod": "Callbacks", "555 AO-Name": "AnalogIO", "PRIME-ShutterMode": "Pre-Exposure", "COM3-Handshaking": "Off", "445 AO-SupportsTriggering": "0", "ROI": "0-0-1200-1200", "COM9-Verbose": "1", "PRIME-Color": "OFF", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "PRIME-ShutterOpenDelay": "0", "COM1-Parity": "None", "Slice": 0, "SlicePosition": -50, "COM9-DelayBetweenCharsMs": "0.0000", "555-Name": "DA Shutter", "PIEZO-LudlSingleAxisName": "B", "XLIGHT Emission Wheel-Label": "679/29 (A)", "640 Shutter-Name": "AnalogIO", "640 AO-Sequenceable": "1", "520 Shutter-Demo": "No", "528 Shutter-Demo": "No", "470-Name": "DA Shutter", "520-DA Device": "520 Shutter", "XYStage-Description": "Ludl XY stage driver adapter", "445 Shutter-MinVolts": "0.0000", "528 AO-TriggerSampleRate": "100000", "405 AO-MinVolts": "0.0000", "405 Shutter-TriggerSequenceLength": "1000", "Height": 1200, "555 AO-Block voltage": "false", "640 Shutter-Volts": "5.0000", "PixelType": "GRAY16", "445 Shutter-Block voltage": "false", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "520 Shutter-TriggerSequenceLength": "1000", "COM9-Name": "COM9", "Core-SLM": "", "555 AO-Volts": "0.0000", "555 Shutter-Block voltage": "false", "XYStage-Speed": "1800.0000", "520 AO-MaxVolts": "5.0000", "528 Shutter-SupportsTriggering": "0", "COM1-Description": "Serial port driver (boost:asio)", "PRIME-MetadataEnabled": "No", "520 Shutter-MinVolts": "0.0000", "470 Shutter-Sequenceable": "1", "PRIME-PreampDelay": "0", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-MinVolts": "0.0000", "ChannelIndex": 2, "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Core-AutoShutter": "1", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "Exposure-ms": 320, "PRIME-PP 2 ENABLED": "Yes", "640 Shutter-TriggerSampleRate": "100000", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "470 Shutter-IOChannel": "Dev1/ao10", "405 AO-Demo": "No", "PixelSizeUm": 1.1, "470 AO-Demo": "No", "470 Shutter-MaxVolts": "5.0000", "Vincent-D3-Name": "Vincent-D3", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FileName": "img_000000000_Il-A_000.tif", "LudlController-Reset": "Operate", "PRIME-Exposure": "320.00", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "445-Name": "DA Shutter", "555 Shutter-Name": "AnalogIO", "520 Shutter-TriggerSampleRate": "100000", "445 Shutter-IOChannel": "Dev1/ao9", "PRIME-TransposeCorrection": "0", "XYStage-StepSize": "0.1000", "445-Description": "DA device that is used as a shutter", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 AO-IOChannel": "Dev1/ao7", "520 Shutter-Name": "AnalogIO", "COM3-DataBits": "8", "640 AO-Volts": "4.5000", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "555 Shutter-IOChannel": "Dev1/ao13", "WaitInterval": null, "520 Shutter-Sequenceable": "1", "YPositionUm": 31506.2, "445-State": "0", "445-DA Device": "445 Shutter", "PRIME-CircularBufferAutoSize": "ON", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "555 AO-TriggerSampleRate": "100000", "445 Shutter-Volts": "0.0000", "XLIGHT Hub-Port": "COM3", "Core-AutoFocus": "", "COM4-Handshaking": "Off", "528 Shutter-MinVolts": "0.0000", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "445 Shutter-Name": "AnalogIO", "528 Shutter-Sequenceable": "1", "XYStage-TransposeMirrorX": "0", "XYStage-TransposeMirrorY": "0", "PRIME-ReadoutRate": "100MHz 16bit", "XYStage-Name": "Stage", "640 AO-SupportsTriggering": "0", "640-Name": "DA Shutter", "640 Shutter-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "640 AO-Block voltage": "false", "445 Shutter-Sequenceable": "1", "470 AO-MaxVolts": "5.0000", "COM4-BaudRate": "9600", "470-Description": "DA device that is used as a shutter", "520 Shutter-Description": "NI DAC", "PRIME-CCDTemperature": "-14.8500", "Time": "2019-12-18 21:36:54 -0500", "640 AO-TriggerSequenceLength": "1000", "470 AO-Name": "AnalogIO", "XLIGHT Disk slider-Label": "Disk Pos 40um", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "Binning": "1x1", "TE2000-ModelType": "1", "COM3-AnswerTimeout": "500.0000", "555 Shutter-SupportsTriggering": "0", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "TE2000-Port": "COM4", "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Description": "NI DAC", "COM4-AnswerTimeout": "500.0000", "COM1-DelayBetweenCharsMs": "0.0000", "FilterBlock-Label": "Confocal", "445 AO-Name": "AnalogIO", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-Timing-ReadoutTimeNs": "24672000.0000", "528 AO-Demo": "No", "520 AO-Sequenceable": "1", "555-Description": "DA device that is used as a shutter", "405-DA Device": "405 Shutter", "405 AO-TriggerSequenceLength": "1000", "640 AO-MinVolts": "0.0000", "640 Shutter-Block voltage": "false", "FrameIndex": 0, "PRIME-Port": "CMOS", "555 AO-MinVolts": "0.0000", "COM3-Description": "Serial port driver (boost:asio)", "528 AO-Description": "NI DAC", "555 AO-IOChannel": "Dev1/ao6", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "555 AO-TriggerInputLine": "/Dev1/PFI0", "PRIME-ActualInterval-ms": "390.5200", "LudlController-Port": "COM9", "445 Shutter-Demo": "No", "PRIME-Trigger-Expose Out-Mux": "1", "SliceIndex": 0, "PRIME-CCDTemperatureSetPoint": "-15.0000", "405 Shutter-MinVolts": "0.0000", "PRIME-ExposeOutMode": "First Row", "AxisPositions": null, "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Volts": "0.0000", "528-Name": "DA Shutter", "555-State": "0", "XLIGHT Dichroic-Label": "Di03-R635-t1", "445 AO-Sequenceable": "1", "405-Name": "DA Shutter", "405 AO-IOChannel": "Dev1/ao0", "Core-Camera": "PRIME", "Focus-Name": "Focus", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "405 Shutter-IOChannel": "Dev1/ao8", "640 AO-Demo": "No", "640 Shutter-SupportsTriggering": "0", "445 AO-Demo": "No", "555-DA Device": "555 Shutter", "470 Shutter-Description": "NI DAC", "528 AO-Volts": "0.0000", "COM1-DataBits": "8", "Vincent-D3-Address": "x", "520 AO-SupportsTriggering": "0", "405-Description": "DA device that is used as a shutter", "640 Shutter-MinVolts": "0.0000", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "405 AO-Name": "AnalogIO", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "640 AO-TriggerInputLine": "/Dev1/PFI0", "405 AO-TriggerInputLine": "/Dev1/PFI0", "640-Description": "DA device that is used as a shutter", "445 Shutter-TriggerSampleRate": "100000", "470 Shutter-TriggerSequenceLength": "1000", "COM1-StopBits": "1", "520 Shutter-SupportsTriggering": "0", "405-State": "0", "Core-Initialize": "1", "XLIGHT Emission Wheel-State": "3", "520 AO-Description": "NI DAC", "528 AO-TriggerInputLine": "/Dev1/PFI0", "445 Shutter-MaxVolts": "5.0000", "COM9-Handshaking": "Off", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "XLIGHT Spin motor-Label": "On (spinning)", "PRIME-PP 5 ENABLED": "No", "UUID": "34cc6e30-7d67-411d-9650-5accb9a695fd", "PRIME-TriggerMode": "Internal Trigger", "COM1-Handshaking": "Off", "PRIME-Timing-PostTriggerDelayNs": "49344000.0000", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "Focus-Position": "17741.5000", "528 Shutter-Block voltage": "false", "Channel": "Il-A", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "555 Shutter-Demo": "No", "405 Shutter-Demo": "No", "528 AO-Name": "AnalogIO", "520-State": "0", "Core-Galvo": "", "405 Shutter-TriggerSampleRate": "100000", "Focus-FocusStepSize_(nm)": "100", "PRIME-PixelType": "16bit", "COM4-DataBits": "8", "470 AO-IOChannel": "Dev1/ao2", "520 AO-MinVolts": "0.0000", "COM9-Parity": "None", "PRIME-CircularBufferEnabled": "ON", "PRIME-PP 6 Reset": "No", "640 AO-Description": "NI DAC", "Source": "PRIME", "445 AO-MinVolts": "0.0000", "PIEZO-Name": "Stage", "445 AO-Description": "NI DAC", "PRIME-CircularBufferFrameCount": "46", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "520 AO-Volts": "0.0000", "528 Shutter-TriggerSampleRate": "100000", "XPositionUm": 19731.9, "OpticalPath-State": "4", "PRIME-PP 3 THRESHOLD": "80", "PRIME-PVCAM Version": "3.7.1", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Gain": "1-HDR", "640 Shutter-TriggerSequenceLength": "1000", "528 AO-SupportsTriggering": "0", "445 AO-TriggerInputLine": "/Dev1/PFI0", "OpticalPath-Name": "OpticalPath", "PRIME-PP 4 ENABLED": "Yes", "PRIME-PVCAM Adapter Version": "1.3.29", "COM3-Name": "COM3", "405 AO-MaxVolts": "5.0000", "520 Shutter-Block voltage": "false", "PRIME-Timing-ClearingTimeNs": "49344000.0000", "Core-Focus": "PIEZO", "528 AO-Block voltage": "false", "TE2000-Description": "Nikon TE2000 hub", "528 AO-MinVolts": "0.0000", "COM4-Parity": "None", "Summary": { "Slices": 11, "ChContrastMin": [ 0, 0, 0, 0, 0, 0 ], "PixelAspect": 1, "SlicesFirst": true, "Interval_ms": 0, "Time": "2019-12-18 21:36:42 -0500", "Prefix": "seq_4", "InitialPositionList": [ { "Label": "Pos0", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.21], "Focus": [17741.5], "XYStage": [ 44542.200000000004, 31636.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos1", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.22], "Focus": [17741.4], "XYStage": [ 43801.100000000006, 31637.9 ] }, "GridColumnIndex": 0 }, { "Label": "Pos2", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.5], "XYStage": [ 38561.3, 32040.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos3", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.185], "Focus": [17741.3], "XYStage": [ 37698.4, 32041.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos4", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.16], "Focus": [17741.5], "XYStage": [ 32375.4, 31758.9 ] }, "GridColumnIndex": 0 }, { "Label": "Pos5", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.17000000000002], "Focus": [17741.3], "XYStage": [ 31770.7, 31758.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos6", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.4], "XYStage": [ 26536.800000000003, 31068.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos7", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.205], "Focus": [17741.4], "XYStage": [ 25420.2, 31068.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos8", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.85], "Focus": [17741.3], "XYStage": [ 21059, 31508 ] }, "GridColumnIndex": 0 }, { "Label": "Pos9", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.82], "Focus": [17741.3], "XYStage": [ 19731.9, 31506.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos10", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.79], "Focus": [17741.4], "XYStage": [ 14528.5, 32007.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos11", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.83], "Focus": [17741.2], "XYStage": [ 13830.300000000001, 32009.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos12", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.79], "Focus": [17741.3], "XYStage": [ 8775.300000000001, 32017.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos13", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.815], "Focus": [17741.3], "XYStage": [ 8109.200000000001, 32008.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos14", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.805], "Focus": [17741.3], "XYStage": [ 3483.3, 31908.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos15", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.82], "Focus": [17741.2], "XYStage": [ 2686.8, 31903.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos16", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [211.48000000000002], "Focus": [17741.1], "XYStage": [ -1978.9, 31973.600000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos17", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [211.51500000000001], "Focus": [17741.1], "XYStage": [ -2957.8, 31982.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos18", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.195], "Focus": [17741.5], "XYStage": [ 47747.4, -576.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos19", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.23000000000002], "Focus": [17741.6], "XYStage": [ 47053.9, -576.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos20", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.205], "Focus": [17741.4], "XYStage": [ 41871.200000000004, -479.8 ] }, "GridColumnIndex": 0 }, { "Label": "Pos21", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.4], "XYStage": [ 40882.600000000006, -392 ] }, "GridColumnIndex": 0 }, { "Label": "Pos22", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.24], "Focus": [17741.4], "XYStage": [ 35905.6, -135.3 ] }, "GridColumnIndex": 0 }, { "Label": "Pos23", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.195], "Focus": [17741.5], "XYStage": [ 34990.9, -132.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos24", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.46], "Focus": [17741.4], "XYStage": [ 29678.100000000002, -958.1 ] }, "GridColumnIndex": 0 }, { "Label": "Pos25", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.44], "Focus": [17741.5], "XYStage": [ 28983.600000000002, -939.4000000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos26", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.475], "Focus": [17741.5], "XYStage": [ 23019.7, -122 ] }, "GridColumnIndex": 0 }, { "Label": "Pos27", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.44], "Focus": [17741.7], "XYStage": [ 22428, -116.80000000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos28", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.46], "Focus": [17741.7], "XYStage": [ 16364.900000000001, -181.10000000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos29", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.48000000000002], "Focus": [17741.5], "XYStage": [ 15712.1, -180 ] }, "GridColumnIndex": 0 }, { "Label": "Pos30", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.45000000000002], "Focus": [17741.7], "XYStage": [ 10346.1, 77.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos31", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.49], "Focus": [17741.4], "XYStage": [ 9755.5, 85.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos32", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.475], "Focus": [17741.5], "XYStage": [ 4775.5, 293.90000000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos33", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.445], "Focus": [17741.4], "XYStage": [ 3954.9, 293.90000000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos34", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.455], "Focus": [17741.4], "XYStage": [ -1324.9, 545.6 ] }, "GridColumnIndex": 0 }, { "Label": "Pos35", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.445], "Focus": [17741.3], "XYStage": [ -2097, 576.6 ] }, "GridColumnIndex": 0 } ], "z-step_um": 10, "GridRow": 0, "BitDepth": 16, "Depth": 2, "Channels": 6, "Source": "Micro-Manager", "ChColors": [ -16711681, -256, -65281, -1, -1, -16776961 ], "MetadataVersion": 10, "KeepShutterOpenChannels": true, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536, 65536 ], "CustomIntervals_ms": [], "Height": 1200, "MicroManagerVersion": "1.4.23 20180219", "GridColumn": 0, "UUID": "14a13bf7-c944-4672-b873-fcfbbb7ea13d", "PixelType": "GRAY16", "Width": 1200, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\Yan\\20191218-YC113-S7S8-miseq", "Frames": 1, "Comment": "", "UserName": "Nikon", "PixelSize_um": 1.1, "ROI": [ 0, 0, 1200, 1200 ], "Date": "2019-12-18", "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC", "P-DAPI-high" ], "KeepShutterOpenSlices": true, "IJType": 1, "ComputerName": "ZadorConfocal2", "Positions": 36, "PositionIndex": 9, "CameraTimeout": 20000, "TimeFirst": false }, "640 AO-Name": "AnalogIO", "Vincent-D3-Command": "Close" }, "FrameKey-0-2-1": { "COM4-StopBits": "1", "528 AO-MaxVolts": "5.0000", "XLIGHT Excitation Wheel-State": "1", "470 Shutter-Name": "AnalogIO", "Core-Shutter": "640", "555 AO-Demo": "No", "XLIGHT Dichroic-State": "2", "XYStage-StepSize-Y": "0.1000", "ZPositionUm": 208.82, "XYStage-StepSize-X": "0.1000", "PRIME-FirmwareVersion": "20.28", "PRIME-ClearCycles": "2", "520-Name": "DA Shutter", "405 Shutter-Name": "AnalogIO", "PIEZO-StepSize": "0.0050", "PRIME-ClearMode": "Pre-Exposure", "470 AO-MinVolts": "0.0000", "405 AO-SupportsTriggering": "1", "CameraChannelIndex": 0, "XYStage-StartSpeed": "500.0000", "COM9-StopBits": "2", "405 AO-Description": "NI DAC", "COM4-DelayBetweenCharsMs": "0.0000", "445 AO-Block voltage": "false", "520 AO-TriggerInputLine": "/Dev1/PFI0", "555 AO-Description": "NI DAC", "520 AO-TriggerSampleRate": "100000", "COM4-Verbose": "1", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM4-Name": "COM4", "405 Shutter-Volts": "0.0000", "Vincent-D3-Port": "COM1", "PRIME-PP 4 MIN ADU AFFECTED": "200", "470 Shutter-MinVolts": "0.0000", "PRIME-PP 3 ENABLED": "Yes", "470 AO-Description": "NI DAC", "PRIME-Description": "PVCAM API device adapter", "520 Shutter-MaxVolts": "5.0000", "520 Shutter-Volts": "0.0000", "528 AO-Sequenceable": "1", "PRIME-Binning": "1x1", "555 Shutter-MaxVolts": "5.0000", "528 Shutter-TriggerSequenceLength": "1000", "405 Shutter-Block voltage": "false", "XLIGHT Spin motor-State": "1", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "Vincent-D3-Time to open (ms)": "35.0000", "Vincent-D3-Time to close (ms)": "35.0000", "445 AO-TriggerSampleRate": "100000", "640 Shutter-IOChannel": "Dev1/ao14", "555 AO-Sequenceable": "1", "520 AO-Name": "AnalogIO", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "470 AO-Block voltage": "false", "COM1-Verbose": "1", "COM9-AnswerTimeout": "10000.0000", "528 Shutter-Name": "AnalogIO", "PRIME-Trigger Timeout (secs)": "10", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "NextFrame": 0, "470 AO-TriggerSequenceLength": "1000", "PRIME-TransposeXY": "0", "445 AO-TriggerSequenceLength": "1000", "COM1-Name": "COM1", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "COM9-DataBits": "8", "COM1-AnswerTimeout": "500.0000", "Camera": "", "TE2000-Version": "V2.50-V2.50-V2.10", "528 Shutter-Volts": "0.0000", "XLIGHT Hub-Name": "XLIGHT Hub", "PIEZO-Description": "Ludl stage driver adapter", "PositionName": "Pos9", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSampleRate": "100000", "PRIME-FanSpeedSetpoint": "High", "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "405 AO-TriggerSampleRate": "100000", "470 Shutter-Block voltage": "false", "470-State": "0", "COM3-Parity": "None", "405 AO-Volts": "0.0000", "PRIME-TransposeMirrorY": "0", "PRIME-ChipName": "GS144BSI", "470 AO-Volts": "0.0000", "PRIME-TransposeMirrorX": "0", "445 Shutter-SupportsTriggering": "0", "470 Shutter-SupportsTriggering": "0", "XLIGHT Hub-Description": "XLIGHT hub", "PRIME-PreampOffLimit": "10000", "470 AO-Sequenceable": "1", "528 Shutter-MaxVolts": "5.0000", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "Core-XYStage": "XYStage", "470 AO-TriggerSampleRate": "100000", "PRIME-Timing-PreTriggerDelayNs": "24672000.0000", "528-DA Device": "528 Shutter", "Focus-Description": "Nikon TE2000 focus adapter", "FilterBlock-State": "5", "520 AO-Demo": "No", "COM3-DelayBetweenCharsMs": "0.0000", "470 Shutter-Volts": "0.0000", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "PRIME-PP 2 THRESHOLD": "125", "PRIME-PP 4 THRESHOLD": "75", "XYStage-Acceleration": "4.0000", "470 Shutter-Demo": "No", "PRIME-Name": "PMPCIECam00", "640-DA Device": "640 Shutter", "405 AO-Block voltage": "false", "ElapsedTime-ms": 387431, "PRIME-Offset": "100", "445 AO-IOChannel": "Dev1/ao1", "640 AO-MaxVolts": "5.0000", "PositionIndex": 9, "PRIME-PP 1 ENABLED": "Yes", "COM1-BaudRate": "9600", "640-State": "1", "COM3-Verbose": "1", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "LudlController-TransmissionDelay": "0", "PRIME-FullWellCapacity": "62000", "PRIME-SMARTStreamingEnabled": "No", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "640 Shutter-Description": "NI DAC", "528-Description": "DA device that is used as a shutter", "COM4-Description": "Serial port driver (boost:asio)", "405 Shutter-Description": "NI DAC", "TE2000-Name": "TE2000", "528 Shutter-Description": "NI DAC", "COM9-Description": "Serial port driver (boost:asio)", "LudlController-Name": "LudlController", "445 Shutter-TriggerSequenceLength": "1000", "PRIME-SerialNumber": "A18B203008", "445 AO-Volts": "0.0000", "PRIME-X-dimension": "1200", "PRIME-ShutterCloseDelay": "0", "XLIGHT Disk slider-State": "2", "Position": "Default", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 AO-IOChannel": "Dev1/ao5", "520 AO-IOChannel": "Dev1/ao4", "528 AO-TriggerSequenceLength": "1000", "405 Shutter-MaxVolts": "5.0000", "470-DA Device": "470 Shutter", "PRIME-FTCapable": "No", "Core-ChannelGroup": "Channels", "555 AO-SupportsTriggering": "0", "PRIME-Y-dimension": "1200", "Frame": 0, "555 Shutter-Sequenceable": "1", "PRIME-Timing-ExposureTimeNs": "499998000.0000", "Core-ImageProcessor": "", "528-State": "0", "520 AO-Block voltage": "false", "XLIGHT Excitation Wheel-Label": "QUAD", "640 Shutter-Demo": "No", "COM3-StopBits": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "520 Shutter-IOChannel": "Dev1/ao11", "Width": 1200, "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-SupportsTriggering": "0", "640 AO-TriggerSampleRate": "100000", "520-Description": "DA device that is used as a shutter", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "405 Shutter-Sequenceable": "1", "405 Shutter-SupportsTriggering": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-TriggerSampleRate": "100000", "640 Shutter-MaxVolts": "5.0000", "555 AO-MaxVolts": "5.0000", "520 AO-TriggerSequenceLength": "1000", "COM3-BaudRate": "9600", "COM9-BaudRate": "9600", "Vincent-D3-Channel #": "Ch. #1", "PRIME-PMode": "Normal", "OpticalPath-Label": "Left(100)", "BitDepth": 16, "555 AO-TriggerSequenceLength": "1000", "445 Shutter-Description": "NI DAC", "405 AO-Sequenceable": "1", "PRIME-AcquisitionMethod": "Callbacks", "555 AO-Name": "AnalogIO", "PRIME-ShutterMode": "Pre-Exposure", "COM3-Handshaking": "Off", "445 AO-SupportsTriggering": "0", "ROI": "0-0-1200-1200", "COM9-Verbose": "1", "PRIME-Color": "OFF", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "PRIME-ShutterOpenDelay": "0", "COM1-Parity": "None", "Slice": 1, "SlicePosition": -40, "COM9-DelayBetweenCharsMs": "0.0000", "555-Name": "DA Shutter", "PIEZO-LudlSingleAxisName": "B", "XLIGHT Emission Wheel-Label": "679/29 (A)", "640 Shutter-Name": "AnalogIO", "640 AO-Sequenceable": "1", "520 Shutter-Demo": "No", "528 Shutter-Demo": "No", "470-Name": "DA Shutter", "520-DA Device": "520 Shutter", "XYStage-Description": "Ludl XY stage driver adapter", "445 Shutter-MinVolts": "0.0000", "528 AO-TriggerSampleRate": "100000", "405 AO-MinVolts": "0.0000", "405 Shutter-TriggerSequenceLength": "1000", "Height": 1200, "555 AO-Block voltage": "false", "640 Shutter-Volts": "5.0000", "PixelType": "GRAY16", "445 Shutter-Block voltage": "false", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "520 Shutter-TriggerSequenceLength": "1000", "COM9-Name": "COM9", "Core-SLM": "", "555 AO-Volts": "0.0000", "555 Shutter-Block voltage": "false", "XYStage-Speed": "1800.0000", "520 AO-MaxVolts": "5.0000", "528 Shutter-SupportsTriggering": "0", "COM1-Description": "Serial port driver (boost:asio)", "PRIME-MetadataEnabled": "No", "520 Shutter-MinVolts": "0.0000", "470 Shutter-Sequenceable": "1", "PRIME-PreampDelay": "0", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-MinVolts": "0.0000", "ChannelIndex": 2, "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Core-AutoShutter": "1", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "Exposure-ms": 320, "PRIME-PP 2 ENABLED": "Yes", "640 Shutter-TriggerSampleRate": "100000", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "470 Shutter-IOChannel": "Dev1/ao10", "405 AO-Demo": "No", "PixelSizeUm": 1.1, "470 AO-Demo": "No", "470 Shutter-MaxVolts": "5.0000", "Vincent-D3-Name": "Vincent-D3", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FileName": "img_000000000_Il-A_001.tif", "LudlController-Reset": "Operate", "PRIME-Exposure": "320.00", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "445-Name": "DA Shutter", "555 Shutter-Name": "AnalogIO", "520 Shutter-TriggerSampleRate": "100000", "445 Shutter-IOChannel": "Dev1/ao9", "PRIME-TransposeCorrection": "0", "XYStage-StepSize": "0.1000", "445-Description": "DA device that is used as a shutter", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 AO-IOChannel": "Dev1/ao7", "520 Shutter-Name": "AnalogIO", "COM3-DataBits": "8", "640 AO-Volts": "4.5000", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "555 Shutter-IOChannel": "Dev1/ao13", "WaitInterval": null, "520 Shutter-Sequenceable": "1", "YPositionUm": 31506.2, "445-State": "0", "445-DA Device": "445 Shutter", "PRIME-CircularBufferAutoSize": "ON", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "555 AO-TriggerSampleRate": "100000", "445 Shutter-Volts": "0.0000", "XLIGHT Hub-Port": "COM3", "Core-AutoFocus": "", "COM4-Handshaking": "Off", "528 Shutter-MinVolts": "0.0000", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "445 Shutter-Name": "AnalogIO", "528 Shutter-Sequenceable": "1", "XYStage-TransposeMirrorX": "0", "XYStage-TransposeMirrorY": "0", "PRIME-ReadoutRate": "100MHz 16bit", "XYStage-Name": "Stage", "640 AO-SupportsTriggering": "0", "640-Name": "DA Shutter", "640 Shutter-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "640 AO-Block voltage": "false", "445 Shutter-Sequenceable": "1", "470 AO-MaxVolts": "5.0000", "COM4-BaudRate": "9600", "470-Description": "DA device that is used as a shutter", "520 Shutter-Description": "NI DAC", "PRIME-CCDTemperature": "-14.8500", "Time": "2019-12-18 21:36:55 -0500", "640 AO-TriggerSequenceLength": "1000", "470 AO-Name": "AnalogIO", "XLIGHT Disk slider-Label": "Disk Pos 40um", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "Binning": "1x1", "TE2000-ModelType": "1", "COM3-AnswerTimeout": "500.0000", "555 Shutter-SupportsTriggering": "0", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "TE2000-Port": "COM4", "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Description": "NI DAC", "COM4-AnswerTimeout": "500.0000", "COM1-DelayBetweenCharsMs": "0.0000", "FilterBlock-Label": "Confocal", "445 AO-Name": "AnalogIO", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-Timing-ReadoutTimeNs": "24672000.0000", "528 AO-Demo": "No", "520 AO-Sequenceable": "1", "555-Description": "DA device that is used as a shutter", "405-DA Device": "405 Shutter", "405 AO-TriggerSequenceLength": "1000", "640 AO-MinVolts": "0.0000", "640 Shutter-Block voltage": "false", "FrameIndex": 0, "PRIME-Port": "CMOS", "555 AO-MinVolts": "0.0000", "COM3-Description": "Serial port driver (boost:asio)", "528 AO-Description": "NI DAC", "555 AO-IOChannel": "Dev1/ao6", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "555 AO-TriggerInputLine": "/Dev1/PFI0", "PRIME-ActualInterval-ms": "390.5200", "LudlController-Port": "COM9", "445 Shutter-Demo": "No", "PRIME-Trigger-Expose Out-Mux": "1", "SliceIndex": 1, "PRIME-CCDTemperatureSetPoint": "-15.0000", "405 Shutter-MinVolts": "0.0000", "PRIME-ExposeOutMode": "First Row", "AxisPositions": null, "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Volts": "0.0000", "528-Name": "DA Shutter", "555-State": "0", "XLIGHT Dichroic-Label": "Di03-R635-t1", "445 AO-Sequenceable": "1", "405-Name": "DA Shutter", "405 AO-IOChannel": "Dev1/ao0", "Core-Camera": "PRIME", "Focus-Name": "Focus", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "405 Shutter-IOChannel": "Dev1/ao8", "640 AO-Demo": "No", "640 Shutter-SupportsTriggering": "0", "445 AO-Demo": "No", "555-DA Device": "555 Shutter", "470 Shutter-Description": "NI DAC", "528 AO-Volts": "0.0000", "COM1-DataBits": "8", "Vincent-D3-Address": "x", "520 AO-SupportsTriggering": "0", "405-Description": "DA device that is used as a shutter", "640 Shutter-MinVolts": "0.0000", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "405 AO-Name": "AnalogIO", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "640 AO-TriggerInputLine": "/Dev1/PFI0", "405 AO-TriggerInputLine": "/Dev1/PFI0", "640-Description": "DA device that is used as a shutter", "445 Shutter-TriggerSampleRate": "100000", "470 Shutter-TriggerSequenceLength": "1000", "COM1-StopBits": "1", "520 Shutter-SupportsTriggering": "0", "405-State": "0", "Core-Initialize": "1", "XLIGHT Emission Wheel-State": "3", "520 AO-Description": "NI DAC", "528 AO-TriggerInputLine": "/Dev1/PFI0", "445 Shutter-MaxVolts": "5.0000", "COM9-Handshaking": "Off", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "XLIGHT Spin motor-Label": "On (spinning)", "PRIME-PP 5 ENABLED": "No", "UUID": "6d864512-88d9-4a6a-adfc-26672b6cb0d9", "PRIME-TriggerMode": "Internal Trigger", "COM1-Handshaking": "Off", "PRIME-Timing-PostTriggerDelayNs": "49344000.0000", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "Focus-Position": "17741.5000", "528 Shutter-Block voltage": "false", "Channel": "Il-A", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "555 Shutter-Demo": "No", "405 Shutter-Demo": "No", "528 AO-Name": "AnalogIO", "520-State": "0", "Core-Galvo": "", "405 Shutter-TriggerSampleRate": "100000", "Focus-FocusStepSize_(nm)": "100", "PRIME-PixelType": "16bit", "COM4-DataBits": "8", "470 AO-IOChannel": "Dev1/ao2", "520 AO-MinVolts": "0.0000", "COM9-Parity": "None", "PRIME-CircularBufferEnabled": "ON", "PRIME-PP 6 Reset": "No", "640 AO-Description": "NI DAC", "Source": "PRIME", "445 AO-MinVolts": "0.0000", "PIEZO-Name": "Stage", "445 AO-Description": "NI DAC", "PRIME-CircularBufferFrameCount": "46", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "520 AO-Volts": "0.0000", "528 Shutter-TriggerSampleRate": "100000", "XPositionUm": 19731.9, "OpticalPath-State": "4", "PRIME-PP 3 THRESHOLD": "80", "PRIME-PVCAM Version": "3.7.1", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Gain": "1-HDR", "640 Shutter-TriggerSequenceLength": "1000", "528 AO-SupportsTriggering": "0", "445 AO-TriggerInputLine": "/Dev1/PFI0", "OpticalPath-Name": "OpticalPath", "PRIME-PP 4 ENABLED": "Yes", "PRIME-PVCAM Adapter Version": "1.3.29", "COM3-Name": "COM3", "405 AO-MaxVolts": "5.0000", "520 Shutter-Block voltage": "false", "PRIME-Timing-ClearingTimeNs": "49344000.0000", "Core-Focus": "PIEZO", "528 AO-Block voltage": "false", "TE2000-Description": "Nikon TE2000 hub", "528 AO-MinVolts": "0.0000", "COM4-Parity": "None", "Summary": { "Slices": 11, "ChContrastMin": [ 0, 0, 0, 0, 0, 0 ], "PixelAspect": 1, "SlicesFirst": true, "Interval_ms": 0, "Time": "2019-12-18 21:36:42 -0500", "Prefix": "seq_4", "InitialPositionList": [ { "Label": "Pos0", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.21], "Focus": [17741.5], "XYStage": [ 44542.200000000004, 31636.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos1", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.22], "Focus": [17741.4], "XYStage": [ 43801.100000000006, 31637.9 ] }, "GridColumnIndex": 0 }, { "Label": "Pos2", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.5], "XYStage": [ 38561.3, 32040.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos3", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.185], "Focus": [17741.3], "XYStage": [ 37698.4, 32041.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos4", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.16], "Focus": [17741.5], "XYStage": [ 32375.4, 31758.9 ] }, "GridColumnIndex": 0 }, { "Label": "Pos5", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.17000000000002], "Focus": [17741.3], "XYStage": [ 31770.7, 31758.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos6", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.4], "XYStage": [ 26536.800000000003, 31068.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos7", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.205], "Focus": [17741.4], "XYStage": [ 25420.2, 31068.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos8", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.85], "Focus": [17741.3], "XYStage": [ 21059, 31508 ] }, "GridColumnIndex": 0 }, { "Label": "Pos9", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.82], "Focus": [17741.3], "XYStage": [ 19731.9, 31506.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos10", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.79], "Focus": [17741.4], "XYStage": [ 14528.5, 32007.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos11", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.83], "Focus": [17741.2], "XYStage": [ 13830.300000000001, 32009.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos12", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.79], "Focus": [17741.3], "XYStage": [ 8775.300000000001, 32017.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos13", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.815], "Focus": [17741.3], "XYStage": [ 8109.200000000001, 32008.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos14", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.805], "Focus": [17741.3], "XYStage": [ 3483.3, 31908.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos15", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.82], "Focus": [17741.2], "XYStage": [ 2686.8, 31903.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos16", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [211.48000000000002], "Focus": [17741.1], "XYStage": [ -1978.9, 31973.600000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos17", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [211.51500000000001], "Focus": [17741.1], "XYStage": [ -2957.8, 31982.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos18", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.195], "Focus": [17741.5], "XYStage": [ 47747.4, -576.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos19", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.23000000000002], "Focus": [17741.6], "XYStage": [ 47053.9, -576.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos20", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.205], "Focus": [17741.4], "XYStage": [ 41871.200000000004, -479.8 ] }, "GridColumnIndex": 0 }, { "Label": "Pos21", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.4], "XYStage": [ 40882.600000000006, -392 ] }, "GridColumnIndex": 0 }, { "Label": "Pos22", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.24], "Focus": [17741.4], "XYStage": [ 35905.6, -135.3 ] }, "GridColumnIndex": 0 }, { "Label": "Pos23", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.195], "Focus": [17741.5], "XYStage": [ 34990.9, -132.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos24", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.46], "Focus": [17741.4], "XYStage": [ 29678.100000000002, -958.1 ] }, "GridColumnIndex": 0 }, { "Label": "Pos25", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.44], "Focus": [17741.5], "XYStage": [ 28983.600000000002, -939.4000000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos26", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.475], "Focus": [17741.5], "XYStage": [ 23019.7, -122 ] }, "GridColumnIndex": 0 }, { "Label": "Pos27", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.44], "Focus": [17741.7], "XYStage": [ 22428, -116.80000000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos28", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.46], "Focus": [17741.7], "XYStage": [ 16364.900000000001, -181.10000000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos29", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.48000000000002], "Focus": [17741.5], "XYStage": [ 15712.1, -180 ] }, "GridColumnIndex": 0 }, { "Label": "Pos30", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.45000000000002], "Focus": [17741.7], "XYStage": [ 10346.1, 77.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos31", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.49], "Focus": [17741.4], "XYStage": [ 9755.5, 85.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos32", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.475], "Focus": [17741.5], "XYStage": [ 4775.5, 293.90000000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos33", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.445], "Focus": [17741.4], "XYStage": [ 3954.9, 293.90000000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos34", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.455], "Focus": [17741.4], "XYStage": [ -1324.9, 545.6 ] }, "GridColumnIndex": 0 }, { "Label": "Pos35", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.445], "Focus": [17741.3], "XYStage": [ -2097, 576.6 ] }, "GridColumnIndex": 0 } ], "z-step_um": 10, "GridRow": 0, "BitDepth": 16, "Depth": 2, "Channels": 6, "Source": "Micro-Manager", "ChColors": [ -16711681, -256, -65281, -1, -1, -16776961 ], "MetadataVersion": 10, "KeepShutterOpenChannels": true, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536, 65536 ], "CustomIntervals_ms": [], "Height": 1200, "MicroManagerVersion": "1.4.23 20180219", "GridColumn": 0, "UUID": "14a13bf7-c944-4672-b873-fcfbbb7ea13d", "PixelType": "GRAY16", "Width": 1200, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\Yan\\20191218-YC113-S7S8-miseq", "Frames": 1, "Comment": "", "UserName": "Nikon", "PixelSize_um": 1.1, "ROI": [ 0, 0, 1200, 1200 ], "Date": "2019-12-18", "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC", "P-DAPI-high" ], "KeepShutterOpenSlices": true, "IJType": 1, "ComputerName": "ZadorConfocal2", "Positions": 36, "PositionIndex": 9, "CameraTimeout": 20000, "TimeFirst": false }, "640 AO-Name": "AnalogIO", "Vincent-D3-Command": "Close" }, "FrameKey-0-2-2": { "COM4-StopBits": "1", "528 AO-MaxVolts": "5.0000", "XLIGHT Excitation Wheel-State": "1", "470 Shutter-Name": "AnalogIO", "Core-Shutter": "640", "555 AO-Demo": "No", "XLIGHT Dichroic-State": "2", "XYStage-StepSize-Y": "0.1000", "ZPositionUm": 218.82, "XYStage-StepSize-X": "0.1000", "PRIME-FirmwareVersion": "20.28", "PRIME-ClearCycles": "2", "520-Name": "DA Shutter", "405 Shutter-Name": "AnalogIO", "PIEZO-StepSize": "0.0050", "PRIME-ClearMode": "Pre-Exposure", "470 AO-MinVolts": "0.0000", "405 AO-SupportsTriggering": "1", "CameraChannelIndex": 0, "XYStage-StartSpeed": "500.0000", "COM9-StopBits": "2", "405 AO-Description": "NI DAC", "COM4-DelayBetweenCharsMs": "0.0000", "445 AO-Block voltage": "false", "520 AO-TriggerInputLine": "/Dev1/PFI0", "555 AO-Description": "NI DAC", "520 AO-TriggerSampleRate": "100000", "COM4-Verbose": "1", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM4-Name": "COM4", "405 Shutter-Volts": "0.0000", "Vincent-D3-Port": "COM1", "PRIME-PP 4 MIN ADU AFFECTED": "200", "470 Shutter-MinVolts": "0.0000", "PRIME-PP 3 ENABLED": "Yes", "470 AO-Description": "NI DAC", "PRIME-Description": "PVCAM API device adapter", "520 Shutter-MaxVolts": "5.0000", "520 Shutter-Volts": "0.0000", "528 AO-Sequenceable": "1", "PRIME-Binning": "1x1", "555 Shutter-MaxVolts": "5.0000", "528 Shutter-TriggerSequenceLength": "1000", "405 Shutter-Block voltage": "false", "XLIGHT Spin motor-State": "1", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "Vincent-D3-Time to open (ms)": "35.0000", "Vincent-D3-Time to close (ms)": "35.0000", "445 AO-TriggerSampleRate": "100000", "640 Shutter-IOChannel": "Dev1/ao14", "555 AO-Sequenceable": "1", "520 AO-Name": "AnalogIO", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "470 AO-Block voltage": "false", "COM1-Verbose": "1", "COM9-AnswerTimeout": "10000.0000", "528 Shutter-Name": "AnalogIO", "PRIME-Trigger Timeout (secs)": "10", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "NextFrame": 0, "470 AO-TriggerSequenceLength": "1000", "PRIME-TransposeXY": "0", "445 AO-TriggerSequenceLength": "1000", "COM1-Name": "COM1", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "COM9-DataBits": "8", "COM1-AnswerTimeout": "500.0000", "Camera": "", "TE2000-Version": "V2.50-V2.50-V2.10", "528 Shutter-Volts": "0.0000", "XLIGHT Hub-Name": "XLIGHT Hub", "PIEZO-Description": "Ludl stage driver adapter", "PositionName": "Pos9", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSampleRate": "100000", "PRIME-FanSpeedSetpoint": "High", "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "405 AO-TriggerSampleRate": "100000", "470 Shutter-Block voltage": "false", "470-State": "0", "COM3-Parity": "None", "405 AO-Volts": "0.0000", "PRIME-TransposeMirrorY": "0", "PRIME-ChipName": "GS144BSI", "470 AO-Volts": "0.0000", "PRIME-TransposeMirrorX": "0", "445 Shutter-SupportsTriggering": "0", "470 Shutter-SupportsTriggering": "0", "XLIGHT Hub-Description": "XLIGHT hub", "PRIME-PreampOffLimit": "10000", "470 AO-Sequenceable": "1", "528 Shutter-MaxVolts": "5.0000", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "Core-XYStage": "XYStage", "470 AO-TriggerSampleRate": "100000", "PRIME-Timing-PreTriggerDelayNs": "24672000.0000", "528-DA Device": "528 Shutter", "Focus-Description": "Nikon TE2000 focus adapter", "FilterBlock-State": "5", "520 AO-Demo": "No", "COM3-DelayBetweenCharsMs": "0.0000", "470 Shutter-Volts": "0.0000", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "PRIME-PP 2 THRESHOLD": "125", "PRIME-PP 4 THRESHOLD": "75", "XYStage-Acceleration": "4.0000", "470 Shutter-Demo": "No", "PRIME-Name": "PMPCIECam00", "640-DA Device": "640 Shutter", "405 AO-Block voltage": "false", "ElapsedTime-ms": 388033, "PRIME-Offset": "100", "445 AO-IOChannel": "Dev1/ao1", "640 AO-MaxVolts": "5.0000", "PositionIndex": 9, "PRIME-PP 1 ENABLED": "Yes", "COM1-BaudRate": "9600", "640-State": "1", "COM3-Verbose": "1", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "LudlController-TransmissionDelay": "0", "PRIME-FullWellCapacity": "62000", "PRIME-SMARTStreamingEnabled": "No", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "640 Shutter-Description": "NI DAC", "528-Description": "DA device that is used as a shutter", "COM4-Description": "Serial port driver (boost:asio)", "405 Shutter-Description": "NI DAC", "TE2000-Name": "TE2000", "528 Shutter-Description": "NI DAC", "COM9-Description": "Serial port driver (boost:asio)", "LudlController-Name": "LudlController", "445 Shutter-TriggerSequenceLength": "1000", "PRIME-SerialNumber": "A18B203008", "445 AO-Volts": "0.0000", "PRIME-X-dimension": "1200", "PRIME-ShutterCloseDelay": "0", "XLIGHT Disk slider-State": "2", "Position": "Default", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 AO-IOChannel": "Dev1/ao5", "520 AO-IOChannel": "Dev1/ao4", "528 AO-TriggerSequenceLength": "1000", "405 Shutter-MaxVolts": "5.0000", "470-DA Device": "470 Shutter", "PRIME-FTCapable": "No", "Core-ChannelGroup": "Channels", "555 AO-SupportsTriggering": "0", "PRIME-Y-dimension": "1200", "Frame": 0, "555 Shutter-Sequenceable": "1", "PRIME-Timing-ExposureTimeNs": "499998000.0000", "Core-ImageProcessor": "", "528-State": "0", "520 AO-Block voltage": "false", "XLIGHT Excitation Wheel-Label": "QUAD", "640 Shutter-Demo": "No", "COM3-StopBits": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "520 Shutter-IOChannel": "Dev1/ao11", "Width": 1200, "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-SupportsTriggering": "0", "640 AO-TriggerSampleRate": "100000", "520-Description": "DA device that is used as a shutter", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "405 Shutter-Sequenceable": "1", "405 Shutter-SupportsTriggering": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-TriggerSampleRate": "100000", "640 Shutter-MaxVolts": "5.0000", "555 AO-MaxVolts": "5.0000", "520 AO-TriggerSequenceLength": "1000", "COM3-BaudRate": "9600", "COM9-BaudRate": "9600", "Vincent-D3-Channel #": "Ch. #1", "PRIME-PMode": "Normal", "OpticalPath-Label": "Left(100)", "BitDepth": 16, "555 AO-TriggerSequenceLength": "1000", "445 Shutter-Description": "NI DAC", "405 AO-Sequenceable": "1", "PRIME-AcquisitionMethod": "Callbacks", "555 AO-Name": "AnalogIO", "PRIME-ShutterMode": "Pre-Exposure", "COM3-Handshaking": "Off", "445 AO-SupportsTriggering": "0", "ROI": "0-0-1200-1200", "COM9-Verbose": "1", "PRIME-Color": "OFF", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "PRIME-ShutterOpenDelay": "0", "COM1-Parity": "None", "Slice": 2, "SlicePosition": -30, "COM9-DelayBetweenCharsMs": "0.0000", "555-Name": "DA Shutter", "PIEZO-LudlSingleAxisName": "B", "XLIGHT Emission Wheel-Label": "679/29 (A)", "640 Shutter-Name": "AnalogIO", "640 AO-Sequenceable": "1", "520 Shutter-Demo": "No", "528 Shutter-Demo": "No", "470-Name": "DA Shutter", "520-DA Device": "520 Shutter", "XYStage-Description": "Ludl XY stage driver adapter", "445 Shutter-MinVolts": "0.0000", "528 AO-TriggerSampleRate": "100000", "405 AO-MinVolts": "0.0000", "405 Shutter-TriggerSequenceLength": "1000", "Height": 1200, "555 AO-Block voltage": "false", "640 Shutter-Volts": "5.0000", "PixelType": "GRAY16", "445 Shutter-Block voltage": "false", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "520 Shutter-TriggerSequenceLength": "1000", "COM9-Name": "COM9", "Core-SLM": "", "555 AO-Volts": "0.0000", "555 Shutter-Block voltage": "false", "XYStage-Speed": "1800.0000", "520 AO-MaxVolts": "5.0000", "528 Shutter-SupportsTriggering": "0", "COM1-Description": "Serial port driver (boost:asio)", "PRIME-MetadataEnabled": "No", "520 Shutter-MinVolts": "0.0000", "470 Shutter-Sequenceable": "1", "PRIME-PreampDelay": "0", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-MinVolts": "0.0000", "ChannelIndex": 2, "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Core-AutoShutter": "1", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "Exposure-ms": 320, "PRIME-PP 2 ENABLED": "Yes", "640 Shutter-TriggerSampleRate": "100000", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "470 Shutter-IOChannel": "Dev1/ao10", "405 AO-Demo": "No", "PixelSizeUm": 1.1, "470 AO-Demo": "No", "470 Shutter-MaxVolts": "5.0000", "Vincent-D3-Name": "Vincent-D3", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FileName": "img_000000000_Il-A_002.tif", "LudlController-Reset": "Operate", "PRIME-Exposure": "320.00", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "445-Name": "DA Shutter", "555 Shutter-Name": "AnalogIO", "520 Shutter-TriggerSampleRate": "100000", "445 Shutter-IOChannel": "Dev1/ao9", "PRIME-TransposeCorrection": "0", "XYStage-StepSize": "0.1000", "445-Description": "DA device that is used as a shutter", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 AO-IOChannel": "Dev1/ao7", "520 Shutter-Name": "AnalogIO", "COM3-DataBits": "8", "640 AO-Volts": "4.5000", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "555 Shutter-IOChannel": "Dev1/ao13", "WaitInterval": null, "520 Shutter-Sequenceable": "1", "YPositionUm": 31506.2, "445-State": "0", "445-DA Device": "445 Shutter", "PRIME-CircularBufferAutoSize": "ON", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "555 AO-TriggerSampleRate": "100000", "445 Shutter-Volts": "0.0000", "XLIGHT Hub-Port": "COM3", "Core-AutoFocus": "", "COM4-Handshaking": "Off", "528 Shutter-MinVolts": "0.0000", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "445 Shutter-Name": "AnalogIO", "528 Shutter-Sequenceable": "1", "XYStage-TransposeMirrorX": "0", "XYStage-TransposeMirrorY": "0", "PRIME-ReadoutRate": "100MHz 16bit", "XYStage-Name": "Stage", "640 AO-SupportsTriggering": "0", "640-Name": "DA Shutter", "640 Shutter-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "640 AO-Block voltage": "false", "445 Shutter-Sequenceable": "1", "470 AO-MaxVolts": "5.0000", "COM4-BaudRate": "9600", "470-Description": "DA device that is used as a shutter", "520 Shutter-Description": "NI DAC", "PRIME-CCDTemperature": "-14.8500", "Time": "2019-12-18 21:36:55 -0500", "640 AO-TriggerSequenceLength": "1000", "470 AO-Name": "AnalogIO", "XLIGHT Disk slider-Label": "Disk Pos 40um", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "Binning": "1x1", "TE2000-ModelType": "1", "COM3-AnswerTimeout": "500.0000", "555 Shutter-SupportsTriggering": "0", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "TE2000-Port": "COM4", "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Description": "NI DAC", "COM4-AnswerTimeout": "500.0000", "COM1-DelayBetweenCharsMs": "0.0000", "FilterBlock-Label": "Confocal", "445 AO-Name": "AnalogIO", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-Timing-ReadoutTimeNs": "24672000.0000", "528 AO-Demo": "No", "520 AO-Sequenceable": "1", "555-Description": "DA device that is used as a shutter", "405-DA Device": "405 Shutter", "405 AO-TriggerSequenceLength": "1000", "640 AO-MinVolts": "0.0000", "640 Shutter-Block voltage": "false", "FrameIndex": 0, "PRIME-Port": "CMOS", "555 AO-MinVolts": "0.0000", "COM3-Description": "Serial port driver (boost:asio)", "528 AO-Description": "NI DAC", "555 AO-IOChannel": "Dev1/ao6", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "555 AO-TriggerInputLine": "/Dev1/PFI0", "PRIME-ActualInterval-ms": "390.5200", "LudlController-Port": "COM9", "445 Shutter-Demo": "No", "PRIME-Trigger-Expose Out-Mux": "1", "SliceIndex": 2, "PRIME-CCDTemperatureSetPoint": "-15.0000", "405 Shutter-MinVolts": "0.0000", "PRIME-ExposeOutMode": "First Row", "AxisPositions": null, "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Volts": "0.0000", "528-Name": "DA Shutter", "555-State": "0", "XLIGHT Dichroic-Label": "Di03-R635-t1", "445 AO-Sequenceable": "1", "405-Name": "DA Shutter", "405 AO-IOChannel": "Dev1/ao0", "Core-Camera": "PRIME", "Focus-Name": "Focus", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "405 Shutter-IOChannel": "Dev1/ao8", "640 AO-Demo": "No", "640 Shutter-SupportsTriggering": "0", "445 AO-Demo": "No", "555-DA Device": "555 Shutter", "470 Shutter-Description": "NI DAC", "528 AO-Volts": "0.0000", "COM1-DataBits": "8", "Vincent-D3-Address": "x", "520 AO-SupportsTriggering": "0", "405-Description": "DA device that is used as a shutter", "640 Shutter-MinVolts": "0.0000", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "405 AO-Name": "AnalogIO", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "640 AO-TriggerInputLine": "/Dev1/PFI0", "405 AO-TriggerInputLine": "/Dev1/PFI0", "640-Description": "DA device that is used as a shutter", "445 Shutter-TriggerSampleRate": "100000", "470 Shutter-TriggerSequenceLength": "1000", "COM1-StopBits": "1", "520 Shutter-SupportsTriggering": "0", "405-State": "0", "Core-Initialize": "1", "XLIGHT Emission Wheel-State": "3", "520 AO-Description": "NI DAC", "528 AO-TriggerInputLine": "/Dev1/PFI0", "445 Shutter-MaxVolts": "5.0000", "COM9-Handshaking": "Off", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "XLIGHT Spin motor-Label": "On (spinning)", "PRIME-PP 5 ENABLED": "No", "UUID": "bad5be23-e547-44e0-95d7-129e10d7262d", "PRIME-TriggerMode": "Internal Trigger", "COM1-Handshaking": "Off", "PRIME-Timing-PostTriggerDelayNs": "49344000.0000", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "Focus-Position": "17741.5000", "528 Shutter-Block voltage": "false", "Channel": "Il-A", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "555 Shutter-Demo": "No", "405 Shutter-Demo": "No", "528 AO-Name": "AnalogIO", "520-State": "0", "Core-Galvo": "", "405 Shutter-TriggerSampleRate": "100000", "Focus-FocusStepSize_(nm)": "100", "PRIME-PixelType": "16bit", "COM4-DataBits": "8", "470 AO-IOChannel": "Dev1/ao2", "520 AO-MinVolts": "0.0000", "COM9-Parity": "None", "PRIME-CircularBufferEnabled": "ON", "PRIME-PP 6 Reset": "No", "640 AO-Description": "NI DAC", "Source": "PRIME", "445 AO-MinVolts": "0.0000", "PIEZO-Name": "Stage", "445 AO-Description": "NI DAC", "PRIME-CircularBufferFrameCount": "46", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "520 AO-Volts": "0.0000", "528 Shutter-TriggerSampleRate": "100000", "XPositionUm": 19731.9, "OpticalPath-State": "4", "PRIME-PP 3 THRESHOLD": "80", "PRIME-PVCAM Version": "3.7.1", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Gain": "1-HDR", "640 Shutter-TriggerSequenceLength": "1000", "528 AO-SupportsTriggering": "0", "445 AO-TriggerInputLine": "/Dev1/PFI0", "OpticalPath-Name": "OpticalPath", "PRIME-PP 4 ENABLED": "Yes", "PRIME-PVCAM Adapter Version": "1.3.29", "COM3-Name": "COM3", "405 AO-MaxVolts": "5.0000", "520 Shutter-Block voltage": "false", "PRIME-Timing-ClearingTimeNs": "49344000.0000", "Core-Focus": "PIEZO", "528 AO-Block voltage": "false", "TE2000-Description": "Nikon TE2000 hub", "528 AO-MinVolts": "0.0000", "COM4-Parity": "None", "Summary": { "Slices": 11, "ChContrastMin": [ 0, 0, 0, 0, 0, 0 ], "PixelAspect": 1, "SlicesFirst": true, "Interval_ms": 0, "Time": "2019-12-18 21:36:42 -0500", "Prefix": "seq_4", "InitialPositionList": [ { "Label": "Pos0", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.21], "Focus": [17741.5], "XYStage": [ 44542.200000000004, 31636.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos1", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.22], "Focus": [17741.4], "XYStage": [ 43801.100000000006, 31637.9 ] }, "GridColumnIndex": 0 }, { "Label": "Pos2", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.5], "XYStage": [ 38561.3, 32040.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos3", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.185], "Focus": [17741.3], "XYStage": [ 37698.4, 32041.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos4", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.16], "Focus": [17741.5], "XYStage": [ 32375.4, 31758.9 ] }, "GridColumnIndex": 0 }, { "Label": "Pos5", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.17000000000002], "Focus": [17741.3], "XYStage": [ 31770.7, 31758.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos6", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.4], "XYStage": [ 26536.800000000003, 31068.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos7", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.205], "Focus": [17741.4], "XYStage": [ 25420.2, 31068.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos8", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.85], "Focus": [17741.3], "XYStage": [ 21059, 31508 ] }, "GridColumnIndex": 0 }, { "Label": "Pos9", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.82], "Focus": [17741.3], "XYStage": [ 19731.9, 31506.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos10", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.79], "Focus": [17741.4], "XYStage": [ 14528.5, 32007.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos11", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.83], "Focus": [17741.2], "XYStage": [ 13830.300000000001, 32009.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos12", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.79], "Focus": [17741.3], "XYStage": [ 8775.300000000001, 32017.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos13", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.815], "Focus": [17741.3], "XYStage": [ 8109.200000000001, 32008.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos14", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.805], "Focus": [17741.3], "XYStage": [ 3483.3, 31908.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos15", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.82], "Focus": [17741.2], "XYStage": [ 2686.8, 31903.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos16", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [211.48000000000002], "Focus": [17741.1], "XYStage": [ -1978.9, 31973.600000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos17", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [211.51500000000001], "Focus": [17741.1], "XYStage": [ -2957.8, 31982.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos18", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.195], "Focus": [17741.5], "XYStage": [ 47747.4, -576.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos19", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.23000000000002], "Focus": [17741.6], "XYStage": [ 47053.9, -576.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos20", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.205], "Focus": [17741.4], "XYStage": [ 41871.200000000004, -479.8 ] }, "GridColumnIndex": 0 }, { "Label": "Pos21", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.4], "XYStage": [ 40882.600000000006, -392 ] }, "GridColumnIndex": 0 }, { "Label": "Pos22", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.24], "Focus": [17741.4], "XYStage": [ 35905.6, -135.3 ] }, "GridColumnIndex": 0 }, { "Label": "Pos23", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.195], "Focus": [17741.5], "XYStage": [ 34990.9, -132.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos24", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.46], "Focus": [17741.4], "XYStage": [ 29678.100000000002, -958.1 ] }, "GridColumnIndex": 0 }, { "Label": "Pos25", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.44], "Focus": [17741.5], "XYStage": [ 28983.600000000002, -939.4000000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos26", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.475], "Focus": [17741.5], "XYStage": [ 23019.7, -122 ] }, "GridColumnIndex": 0 }, { "Label": "Pos27", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.44], "Focus": [17741.7], "XYStage": [ 22428, -116.80000000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos28", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.46], "Focus": [17741.7], "XYStage": [ 16364.900000000001, -181.10000000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos29", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.48000000000002], "Focus": [17741.5], "XYStage": [ 15712.1, -180 ] }, "GridColumnIndex": 0 }, { "Label": "Pos30", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.45000000000002], "Focus": [17741.7], "XYStage": [ 10346.1, 77.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos31", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.49], "Focus": [17741.4], "XYStage": [ 9755.5, 85.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos32", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.475], "Focus": [17741.5], "XYStage": [ 4775.5, 293.90000000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos33", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.445], "Focus": [17741.4], "XYStage": [ 3954.9, 293.90000000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos34", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.455], "Focus": [17741.4], "XYStage": [ -1324.9, 545.6 ] }, "GridColumnIndex": 0 }, { "Label": "Pos35", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.445], "Focus": [17741.3], "XYStage": [ -2097, 576.6 ] }, "GridColumnIndex": 0 } ], "z-step_um": 10, "GridRow": 0, "BitDepth": 16, "Depth": 2, "Channels": 6, "Source": "Micro-Manager", "ChColors": [ -16711681, -256, -65281, -1, -1, -16776961 ], "MetadataVersion": 10, "KeepShutterOpenChannels": true, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536, 65536 ], "CustomIntervals_ms": [], "Height": 1200, "MicroManagerVersion": "1.4.23 20180219", "GridColumn": 0, "UUID": "14a13bf7-c944-4672-b873-fcfbbb7ea13d", "PixelType": "GRAY16", "Width": 1200, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\Yan\\20191218-YC113-S7S8-miseq", "Frames": 1, "Comment": "", "UserName": "Nikon", "PixelSize_um": 1.1, "ROI": [ 0, 0, 1200, 1200 ], "Date": "2019-12-18", "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC", "P-DAPI-high" ], "KeepShutterOpenSlices": true, "IJType": 1, "ComputerName": "ZadorConfocal2", "Positions": 36, "PositionIndex": 9, "CameraTimeout": 20000, "TimeFirst": false }, "640 AO-Name": "AnalogIO", "Vincent-D3-Command": "Close" }, "FrameKey-0-2-3": { "COM4-StopBits": "1", "528 AO-MaxVolts": "5.0000", "XLIGHT Excitation Wheel-State": "1", "470 Shutter-Name": "AnalogIO", "Core-Shutter": "640", "555 AO-Demo": "No", "XLIGHT Dichroic-State": "2", "XYStage-StepSize-Y": "0.1000", "ZPositionUm": 228.82, "XYStage-StepSize-X": "0.1000", "PRIME-FirmwareVersion": "20.28", "PRIME-ClearCycles": "2", "520-Name": "DA Shutter", "405 Shutter-Name": "AnalogIO", "PIEZO-StepSize": "0.0050", "PRIME-ClearMode": "Pre-Exposure", "470 AO-MinVolts": "0.0000", "405 AO-SupportsTriggering": "1", "CameraChannelIndex": 0, "XYStage-StartSpeed": "500.0000", "COM9-StopBits": "2", "405 AO-Description": "NI DAC", "COM4-DelayBetweenCharsMs": "0.0000", "445 AO-Block voltage": "false", "520 AO-TriggerInputLine": "/Dev1/PFI0", "555 AO-Description": "NI DAC", "520 AO-TriggerSampleRate": "100000", "COM4-Verbose": "1", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM4-Name": "COM4", "405 Shutter-Volts": "0.0000", "Vincent-D3-Port": "COM1", "PRIME-PP 4 MIN ADU AFFECTED": "200", "470 Shutter-MinVolts": "0.0000", "PRIME-PP 3 ENABLED": "Yes", "470 AO-Description": "NI DAC", "PRIME-Description": "PVCAM API device adapter", "520 Shutter-MaxVolts": "5.0000", "520 Shutter-Volts": "0.0000", "528 AO-Sequenceable": "1", "PRIME-Binning": "1x1", "555 Shutter-MaxVolts": "5.0000", "528 Shutter-TriggerSequenceLength": "1000", "405 Shutter-Block voltage": "false", "XLIGHT Spin motor-State": "1", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "Vincent-D3-Time to open (ms)": "35.0000", "Vincent-D3-Time to close (ms)": "35.0000", "445 AO-TriggerSampleRate": "100000", "640 Shutter-IOChannel": "Dev1/ao14", "555 AO-Sequenceable": "1", "520 AO-Name": "AnalogIO", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "470 AO-Block voltage": "false", "COM1-Verbose": "1", "COM9-AnswerTimeout": "10000.0000", "528 Shutter-Name": "AnalogIO", "PRIME-Trigger Timeout (secs)": "10", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "NextFrame": 0, "470 AO-TriggerSequenceLength": "1000", "PRIME-TransposeXY": "0", "445 AO-TriggerSequenceLength": "1000", "COM1-Name": "COM1", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "COM9-DataBits": "8", "COM1-AnswerTimeout": "500.0000", "Camera": "", "TE2000-Version": "V2.50-V2.50-V2.10", "528 Shutter-Volts": "0.0000", "XLIGHT Hub-Name": "XLIGHT Hub", "PIEZO-Description": "Ludl stage driver adapter", "PositionName": "Pos9", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSampleRate": "100000", "PRIME-FanSpeedSetpoint": "High", "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "405 AO-TriggerSampleRate": "100000", "470 Shutter-Block voltage": "false", "470-State": "0", "COM3-Parity": "None", "405 AO-Volts": "0.0000", "PRIME-TransposeMirrorY": "0", "PRIME-ChipName": "GS144BSI", "470 AO-Volts": "0.0000", "PRIME-TransposeMirrorX": "0", "445 Shutter-SupportsTriggering": "0", "470 Shutter-SupportsTriggering": "0", "XLIGHT Hub-Description": "XLIGHT hub", "PRIME-PreampOffLimit": "10000", "470 AO-Sequenceable": "1", "528 Shutter-MaxVolts": "5.0000", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "Core-XYStage": "XYStage", "470 AO-TriggerSampleRate": "100000", "PRIME-Timing-PreTriggerDelayNs": "24672000.0000", "528-DA Device": "528 Shutter", "Focus-Description": "Nikon TE2000 focus adapter", "FilterBlock-State": "5", "520 AO-Demo": "No", "COM3-DelayBetweenCharsMs": "0.0000", "470 Shutter-Volts": "0.0000", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "PRIME-PP 2 THRESHOLD": "125", "PRIME-PP 4 THRESHOLD": "75", "XYStage-Acceleration": "4.0000", "470 Shutter-Demo": "No", "PRIME-Name": "PMPCIECam00", "640-DA Device": "640 Shutter", "405 AO-Block voltage": "false", "ElapsedTime-ms": 388604, "PRIME-Offset": "100", "445 AO-IOChannel": "Dev1/ao1", "640 AO-MaxVolts": "5.0000", "PositionIndex": 9, "PRIME-PP 1 ENABLED": "Yes", "COM1-BaudRate": "9600", "640-State": "1", "COM3-Verbose": "1", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "LudlController-TransmissionDelay": "0", "PRIME-FullWellCapacity": "62000", "PRIME-SMARTStreamingEnabled": "No", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "640 Shutter-Description": "NI DAC", "528-Description": "DA device that is used as a shutter", "COM4-Description": "Serial port driver (boost:asio)", "405 Shutter-Description": "NI DAC", "TE2000-Name": "TE2000", "528 Shutter-Description": "NI DAC", "COM9-Description": "Serial port driver (boost:asio)", "LudlController-Name": "LudlController", "445 Shutter-TriggerSequenceLength": "1000", "PRIME-SerialNumber": "A18B203008", "445 AO-Volts": "0.0000", "PRIME-X-dimension": "1200", "PRIME-ShutterCloseDelay": "0", "XLIGHT Disk slider-State": "2", "Position": "Default", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 AO-IOChannel": "Dev1/ao5", "520 AO-IOChannel": "Dev1/ao4", "528 AO-TriggerSequenceLength": "1000", "405 Shutter-MaxVolts": "5.0000", "470-DA Device": "470 Shutter", "PRIME-FTCapable": "No", "Core-ChannelGroup": "Channels", "555 AO-SupportsTriggering": "0", "PRIME-Y-dimension": "1200", "Frame": 0, "555 Shutter-Sequenceable": "1", "PRIME-Timing-ExposureTimeNs": "499998000.0000", "Core-ImageProcessor": "", "528-State": "0", "520 AO-Block voltage": "false", "XLIGHT Excitation Wheel-Label": "QUAD", "640 Shutter-Demo": "No", "COM3-StopBits": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "520 Shutter-IOChannel": "Dev1/ao11", "Width": 1200, "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-SupportsTriggering": "0", "640 AO-TriggerSampleRate": "100000", "520-Description": "DA device that is used as a shutter", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "405 Shutter-Sequenceable": "1", "405 Shutter-SupportsTriggering": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-TriggerSampleRate": "100000", "640 Shutter-MaxVolts": "5.0000", "555 AO-MaxVolts": "5.0000", "520 AO-TriggerSequenceLength": "1000", "COM3-BaudRate": "9600", "COM9-BaudRate": "9600", "Vincent-D3-Channel #": "Ch. #1", "PRIME-PMode": "Normal", "OpticalPath-Label": "Left(100)", "BitDepth": 16, "555 AO-TriggerSequenceLength": "1000", "445 Shutter-Description": "NI DAC", "405 AO-Sequenceable": "1", "PRIME-AcquisitionMethod": "Callbacks", "555 AO-Name": "AnalogIO", "PRIME-ShutterMode": "Pre-Exposure", "COM3-Handshaking": "Off", "445 AO-SupportsTriggering": "0", "ROI": "0-0-1200-1200", "COM9-Verbose": "1", "PRIME-Color": "OFF", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "PRIME-ShutterOpenDelay": "0", "COM1-Parity": "None", "Slice": 3, "SlicePosition": -20, "COM9-DelayBetweenCharsMs": "0.0000", "555-Name": "DA Shutter", "PIEZO-LudlSingleAxisName": "B", "XLIGHT Emission Wheel-Label": "679/29 (A)", "640 Shutter-Name": "AnalogIO", "640 AO-Sequenceable": "1", "520 Shutter-Demo": "No", "528 Shutter-Demo": "No", "470-Name": "DA Shutter", "520-DA Device": "520 Shutter", "XYStage-Description": "Ludl XY stage driver adapter", "445 Shutter-MinVolts": "0.0000", "528 AO-TriggerSampleRate": "100000", "405 AO-MinVolts": "0.0000", "405 Shutter-TriggerSequenceLength": "1000", "Height": 1200, "555 AO-Block voltage": "false", "640 Shutter-Volts": "5.0000", "PixelType": "GRAY16", "445 Shutter-Block voltage": "false", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "520 Shutter-TriggerSequenceLength": "1000", "COM9-Name": "COM9", "Core-SLM": "", "555 AO-Volts": "0.0000", "555 Shutter-Block voltage": "false", "XYStage-Speed": "1800.0000", "520 AO-MaxVolts": "5.0000", "528 Shutter-SupportsTriggering": "0", "COM1-Description": "Serial port driver (boost:asio)", "PRIME-MetadataEnabled": "No", "520 Shutter-MinVolts": "0.0000", "470 Shutter-Sequenceable": "1", "PRIME-PreampDelay": "0", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-MinVolts": "0.0000", "ChannelIndex": 2, "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Core-AutoShutter": "1", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "Exposure-ms": 320, "PRIME-PP 2 ENABLED": "Yes", "640 Shutter-TriggerSampleRate": "100000", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "470 Shutter-IOChannel": "Dev1/ao10", "405 AO-Demo": "No", "PixelSizeUm": 1.1, "470 AO-Demo": "No", "470 Shutter-MaxVolts": "5.0000", "Vincent-D3-Name": "Vincent-D3", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FileName": "img_000000000_Il-A_003.tif", "LudlController-Reset": "Operate", "PRIME-Exposure": "320.00", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "445-Name": "DA Shutter", "555 Shutter-Name": "AnalogIO", "520 Shutter-TriggerSampleRate": "100000", "445 Shutter-IOChannel": "Dev1/ao9", "PRIME-TransposeCorrection": "0", "XYStage-StepSize": "0.1000", "445-Description": "DA device that is used as a shutter", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 AO-IOChannel": "Dev1/ao7", "520 Shutter-Name": "AnalogIO", "COM3-DataBits": "8", "640 AO-Volts": "4.5000", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "555 Shutter-IOChannel": "Dev1/ao13", "WaitInterval": null, "520 Shutter-Sequenceable": "1", "YPositionUm": 31506.2, "445-State": "0", "445-DA Device": "445 Shutter", "PRIME-CircularBufferAutoSize": "ON", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "555 AO-TriggerSampleRate": "100000", "445 Shutter-Volts": "0.0000", "XLIGHT Hub-Port": "COM3", "Core-AutoFocus": "", "COM4-Handshaking": "Off", "528 Shutter-MinVolts": "0.0000", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "445 Shutter-Name": "AnalogIO", "528 Shutter-Sequenceable": "1", "XYStage-TransposeMirrorX": "0", "XYStage-TransposeMirrorY": "0", "PRIME-ReadoutRate": "100MHz 16bit", "XYStage-Name": "Stage", "640 AO-SupportsTriggering": "0", "640-Name": "DA Shutter", "640 Shutter-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "640 AO-Block voltage": "false", "445 Shutter-Sequenceable": "1", "470 AO-MaxVolts": "5.0000", "COM4-BaudRate": "9600", "470-Description": "DA device that is used as a shutter", "520 Shutter-Description": "NI DAC", "PRIME-CCDTemperature": "-14.8500", "Time": "2019-12-18 21:36:56 -0500", "640 AO-TriggerSequenceLength": "1000", "470 AO-Name": "AnalogIO", "XLIGHT Disk slider-Label": "Disk Pos 40um", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "Binning": "1x1", "TE2000-ModelType": "1", "COM3-AnswerTimeout": "500.0000", "555 Shutter-SupportsTriggering": "0", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "TE2000-Port": "COM4", "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Description": "NI DAC", "COM4-AnswerTimeout": "500.0000", "COM1-DelayBetweenCharsMs": "0.0000", "FilterBlock-Label": "Confocal", "445 AO-Name": "AnalogIO", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-Timing-ReadoutTimeNs": "24672000.0000", "528 AO-Demo": "No", "520 AO-Sequenceable": "1", "555-Description": "DA device that is used as a shutter", "405-DA Device": "405 Shutter", "405 AO-TriggerSequenceLength": "1000", "640 AO-MinVolts": "0.0000", "640 Shutter-Block voltage": "false", "FrameIndex": 0, "PRIME-Port": "CMOS", "555 AO-MinVolts": "0.0000", "COM3-Description": "Serial port driver (boost:asio)", "528 AO-Description": "NI DAC", "555 AO-IOChannel": "Dev1/ao6", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "555 AO-TriggerInputLine": "/Dev1/PFI0", "PRIME-ActualInterval-ms": "390.5200", "LudlController-Port": "COM9", "445 Shutter-Demo": "No", "PRIME-Trigger-Expose Out-Mux": "1", "SliceIndex": 3, "PRIME-CCDTemperatureSetPoint": "-15.0000", "405 Shutter-MinVolts": "0.0000", "PRIME-ExposeOutMode": "First Row", "AxisPositions": null, "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Volts": "0.0000", "528-Name": "DA Shutter", "555-State": "0", "XLIGHT Dichroic-Label": "Di03-R635-t1", "445 AO-Sequenceable": "1", "405-Name": "DA Shutter", "405 AO-IOChannel": "Dev1/ao0", "Core-Camera": "PRIME", "Focus-Name": "Focus", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "405 Shutter-IOChannel": "Dev1/ao8", "640 AO-Demo": "No", "640 Shutter-SupportsTriggering": "0", "445 AO-Demo": "No", "555-DA Device": "555 Shutter", "470 Shutter-Description": "NI DAC", "528 AO-Volts": "0.0000", "COM1-DataBits": "8", "Vincent-D3-Address": "x", "520 AO-SupportsTriggering": "0", "405-Description": "DA device that is used as a shutter", "640 Shutter-MinVolts": "0.0000", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "405 AO-Name": "AnalogIO", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "640 AO-TriggerInputLine": "/Dev1/PFI0", "405 AO-TriggerInputLine": "/Dev1/PFI0", "640-Description": "DA device that is used as a shutter", "445 Shutter-TriggerSampleRate": "100000", "470 Shutter-TriggerSequenceLength": "1000", "COM1-StopBits": "1", "520 Shutter-SupportsTriggering": "0", "405-State": "0", "Core-Initialize": "1", "XLIGHT Emission Wheel-State": "3", "520 AO-Description": "NI DAC", "528 AO-TriggerInputLine": "/Dev1/PFI0", "445 Shutter-MaxVolts": "5.0000", "COM9-Handshaking": "Off", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "XLIGHT Spin motor-Label": "On (spinning)", "PRIME-PP 5 ENABLED": "No", "UUID": "998df038-53eb-4c57-89f4-8c386b247a42", "PRIME-TriggerMode": "Internal Trigger", "COM1-Handshaking": "Off", "PRIME-Timing-PostTriggerDelayNs": "49344000.0000", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "Focus-Position": "17741.5000", "528 Shutter-Block voltage": "false", "Channel": "Il-A", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "555 Shutter-Demo": "No", "405 Shutter-Demo": "No", "528 AO-Name": "AnalogIO", "520-State": "0", "Core-Galvo": "", "405 Shutter-TriggerSampleRate": "100000", "Focus-FocusStepSize_(nm)": "100", "PRIME-PixelType": "16bit", "COM4-DataBits": "8", "470 AO-IOChannel": "Dev1/ao2", "520 AO-MinVolts": "0.0000", "COM9-Parity": "None", "PRIME-CircularBufferEnabled": "ON", "PRIME-PP 6 Reset": "No", "640 AO-Description": "NI DAC", "Source": "PRIME", "445 AO-MinVolts": "0.0000", "PIEZO-Name": "Stage", "445 AO-Description": "NI DAC", "PRIME-CircularBufferFrameCount": "46", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "520 AO-Volts": "0.0000", "528 Shutter-TriggerSampleRate": "100000", "XPositionUm": 19731.9, "OpticalPath-State": "4", "PRIME-PP 3 THRESHOLD": "80", "PRIME-PVCAM Version": "3.7.1", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Gain": "1-HDR", "640 Shutter-TriggerSequenceLength": "1000", "528 AO-SupportsTriggering": "0", "445 AO-TriggerInputLine": "/Dev1/PFI0", "OpticalPath-Name": "OpticalPath", "PRIME-PP 4 ENABLED": "Yes", "PRIME-PVCAM Adapter Version": "1.3.29", "COM3-Name": "COM3", "405 AO-MaxVolts": "5.0000", "520 Shutter-Block voltage": "false", "PRIME-Timing-ClearingTimeNs": "49344000.0000", "Core-Focus": "PIEZO", "528 AO-Block voltage": "false", "TE2000-Description": "Nikon TE2000 hub", "528 AO-MinVolts": "0.0000", "COM4-Parity": "None", "Summary": { "Slices": 11, "ChContrastMin": [ 0, 0, 0, 0, 0, 0 ], "PixelAspect": 1, "SlicesFirst": true, "Interval_ms": 0, "Time": "2019-12-18 21:36:42 -0500", "Prefix": "seq_4", "InitialPositionList": [ { "Label": "Pos0", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.21], "Focus": [17741.5], "XYStage": [ 44542.200000000004, 31636.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos1", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.22], "Focus": [17741.4], "XYStage": [ 43801.100000000006, 31637.9 ] }, "GridColumnIndex": 0 }, { "Label": "Pos2", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.5], "XYStage": [ 38561.3, 32040.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos3", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.185], "Focus": [17741.3], "XYStage": [ 37698.4, 32041.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos4", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.16], "Focus": [17741.5], "XYStage": [ 32375.4, 31758.9 ] }, "GridColumnIndex": 0 }, { "Label": "Pos5", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.17000000000002], "Focus": [17741.3], "XYStage": [ 31770.7, 31758.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos6", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.4], "XYStage": [ 26536.800000000003, 31068.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos7", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.205], "Focus": [17741.4], "XYStage": [ 25420.2, 31068.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos8", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.85], "Focus": [17741.3], "XYStage": [ 21059, 31508 ] }, "GridColumnIndex": 0 }, { "Label": "Pos9", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.82], "Focus": [17741.3], "XYStage": [ 19731.9, 31506.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos10", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.79], "Focus": [17741.4], "XYStage": [ 14528.5, 32007.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos11", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.83], "Focus": [17741.2], "XYStage": [ 13830.300000000001, 32009.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos12", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.79], "Focus": [17741.3], "XYStage": [ 8775.300000000001, 32017.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos13", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.815], "Focus": [17741.3], "XYStage": [ 8109.200000000001, 32008.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos14", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.805], "Focus": [17741.3], "XYStage": [ 3483.3, 31908.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos15", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.82], "Focus": [17741.2], "XYStage": [ 2686.8, 31903.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos16", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [211.48000000000002], "Focus": [17741.1], "XYStage": [ -1978.9, 31973.600000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos17", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [211.51500000000001], "Focus": [17741.1], "XYStage": [ -2957.8, 31982.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos18", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.195], "Focus": [17741.5], "XYStage": [ 47747.4, -576.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos19", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.23000000000002], "Focus": [17741.6], "XYStage": [ 47053.9, -576.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos20", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.205], "Focus": [17741.4], "XYStage": [ 41871.200000000004, -479.8 ] }, "GridColumnIndex": 0 }, { "Label": "Pos21", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.4], "XYStage": [ 40882.600000000006, -392 ] }, "GridColumnIndex": 0 }, { "Label": "Pos22", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.24], "Focus": [17741.4], "XYStage": [ 35905.6, -135.3 ] }, "GridColumnIndex": 0 }, { "Label": "Pos23", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.195], "Focus": [17741.5], "XYStage": [ 34990.9, -132.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos24", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.46], "Focus": [17741.4], "XYStage": [ 29678.100000000002, -958.1 ] }, "GridColumnIndex": 0 }, { "Label": "Pos25", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.44], "Focus": [17741.5], "XYStage": [ 28983.600000000002, -939.4000000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos26", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.475], "Focus": [17741.5], "XYStage": [ 23019.7, -122 ] }, "GridColumnIndex": 0 }, { "Label": "Pos27", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.44], "Focus": [17741.7], "XYStage": [ 22428, -116.80000000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos28", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.46], "Focus": [17741.7], "XYStage": [ 16364.900000000001, -181.10000000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos29", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.48000000000002], "Focus": [17741.5], "XYStage": [ 15712.1, -180 ] }, "GridColumnIndex": 0 }, { "Label": "Pos30", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.45000000000002], "Focus": [17741.7], "XYStage": [ 10346.1, 77.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos31", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.49], "Focus": [17741.4], "XYStage": [ 9755.5, 85.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos32", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.475], "Focus": [17741.5], "XYStage": [ 4775.5, 293.90000000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos33", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.445], "Focus": [17741.4], "XYStage": [ 3954.9, 293.90000000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos34", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.455], "Focus": [17741.4], "XYStage": [ -1324.9, 545.6 ] }, "GridColumnIndex": 0 }, { "Label": "Pos35", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.445], "Focus": [17741.3], "XYStage": [ -2097, 576.6 ] }, "GridColumnIndex": 0 } ], "z-step_um": 10, "GridRow": 0, "BitDepth": 16, "Depth": 2, "Channels": 6, "Source": "Micro-Manager", "ChColors": [ -16711681, -256, -65281, -1, -1, -16776961 ], "MetadataVersion": 10, "KeepShutterOpenChannels": true, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536, 65536 ], "CustomIntervals_ms": [], "Height": 1200, "MicroManagerVersion": "1.4.23 20180219", "GridColumn": 0, "UUID": "14a13bf7-c944-4672-b873-fcfbbb7ea13d", "PixelType": "GRAY16", "Width": 1200, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\Yan\\20191218-YC113-S7S8-miseq", "Frames": 1, "Comment": "", "UserName": "Nikon", "PixelSize_um": 1.1, "ROI": [ 0, 0, 1200, 1200 ], "Date": "2019-12-18", "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC", "P-DAPI-high" ], "KeepShutterOpenSlices": true, "IJType": 1, "ComputerName": "ZadorConfocal2", "Positions": 36, "PositionIndex": 9, "CameraTimeout": 20000, "TimeFirst": false }, "640 AO-Name": "AnalogIO", "Vincent-D3-Command": "Close" }, "FrameKey-0-2-4": { "COM4-StopBits": "1", "528 AO-MaxVolts": "5.0000", "XLIGHT Excitation Wheel-State": "1", "470 Shutter-Name": "AnalogIO", "Core-Shutter": "640", "555 AO-Demo": "No", "XLIGHT Dichroic-State": "2", "XYStage-StepSize-Y": "0.1000", "ZPositionUm": 238.82, "XYStage-StepSize-X": "0.1000", "PRIME-FirmwareVersion": "20.28", "PRIME-ClearCycles": "2", "520-Name": "DA Shutter", "405 Shutter-Name": "AnalogIO", "PIEZO-StepSize": "0.0050", "PRIME-ClearMode": "Pre-Exposure", "470 AO-MinVolts": "0.0000", "405 AO-SupportsTriggering": "1", "CameraChannelIndex": 0, "XYStage-StartSpeed": "500.0000", "COM9-StopBits": "2", "405 AO-Description": "NI DAC", "COM4-DelayBetweenCharsMs": "0.0000", "445 AO-Block voltage": "false", "520 AO-TriggerInputLine": "/Dev1/PFI0", "555 AO-Description": "NI DAC", "520 AO-TriggerSampleRate": "100000", "COM4-Verbose": "1", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM4-Name": "COM4", "405 Shutter-Volts": "0.0000", "Vincent-D3-Port": "COM1", "PRIME-PP 4 MIN ADU AFFECTED": "200", "470 Shutter-MinVolts": "0.0000", "PRIME-PP 3 ENABLED": "Yes", "470 AO-Description": "NI DAC", "PRIME-Description": "PVCAM API device adapter", "520 Shutter-MaxVolts": "5.0000", "520 Shutter-Volts": "0.0000", "528 AO-Sequenceable": "1", "PRIME-Binning": "1x1", "555 Shutter-MaxVolts": "5.0000", "528 Shutter-TriggerSequenceLength": "1000", "405 Shutter-Block voltage": "false", "XLIGHT Spin motor-State": "1", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "Vincent-D3-Time to open (ms)": "35.0000", "Vincent-D3-Time to close (ms)": "35.0000", "445 AO-TriggerSampleRate": "100000", "640 Shutter-IOChannel": "Dev1/ao14", "555 AO-Sequenceable": "1", "520 AO-Name": "AnalogIO", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "470 AO-Block voltage": "false", "COM1-Verbose": "1", "COM9-AnswerTimeout": "10000.0000", "528 Shutter-Name": "AnalogIO", "PRIME-Trigger Timeout (secs)": "10", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "NextFrame": 0, "470 AO-TriggerSequenceLength": "1000", "PRIME-TransposeXY": "0", "445 AO-TriggerSequenceLength": "1000", "COM1-Name": "COM1", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "COM9-DataBits": "8", "COM1-AnswerTimeout": "500.0000", "Camera": "", "TE2000-Version": "V2.50-V2.50-V2.10", "528 Shutter-Volts": "0.0000", "XLIGHT Hub-Name": "XLIGHT Hub", "PIEZO-Description": "Ludl stage driver adapter", "PositionName": "Pos9", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSampleRate": "100000", "PRIME-FanSpeedSetpoint": "High", "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "405 AO-TriggerSampleRate": "100000", "470 Shutter-Block voltage": "false", "470-State": "0", "COM3-Parity": "None", "405 AO-Volts": "0.0000", "PRIME-TransposeMirrorY": "0", "PRIME-ChipName": "GS144BSI", "470 AO-Volts": "0.0000", "PRIME-TransposeMirrorX": "0", "445 Shutter-SupportsTriggering": "0", "470 Shutter-SupportsTriggering": "0", "XLIGHT Hub-Description": "XLIGHT hub", "PRIME-PreampOffLimit": "10000", "470 AO-Sequenceable": "1", "528 Shutter-MaxVolts": "5.0000", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "Core-XYStage": "XYStage", "470 AO-TriggerSampleRate": "100000", "PRIME-Timing-PreTriggerDelayNs": "24672000.0000", "528-DA Device": "528 Shutter", "Focus-Description": "Nikon TE2000 focus adapter", "FilterBlock-State": "5", "520 AO-Demo": "No", "COM3-DelayBetweenCharsMs": "0.0000", "470 Shutter-Volts": "0.0000", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "PRIME-PP 2 THRESHOLD": "125", "PRIME-PP 4 THRESHOLD": "75", "XYStage-Acceleration": "4.0000", "470 Shutter-Demo": "No", "PRIME-Name": "PMPCIECam00", "640-DA Device": "640 Shutter", "405 AO-Block voltage": "false", "ElapsedTime-ms": 389210, "PRIME-Offset": "100", "445 AO-IOChannel": "Dev1/ao1", "640 AO-MaxVolts": "5.0000", "PositionIndex": 9, "PRIME-PP 1 ENABLED": "Yes", "COM1-BaudRate": "9600", "640-State": "1", "COM3-Verbose": "1", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "LudlController-TransmissionDelay": "0", "PRIME-FullWellCapacity": "62000", "PRIME-SMARTStreamingEnabled": "No", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "640 Shutter-Description": "NI DAC", "528-Description": "DA device that is used as a shutter", "COM4-Description": "Serial port driver (boost:asio)", "405 Shutter-Description": "NI DAC", "TE2000-Name": "TE2000", "528 Shutter-Description": "NI DAC", "COM9-Description": "Serial port driver (boost:asio)", "LudlController-Name": "LudlController", "445 Shutter-TriggerSequenceLength": "1000", "PRIME-SerialNumber": "A18B203008", "445 AO-Volts": "0.0000", "PRIME-X-dimension": "1200", "PRIME-ShutterCloseDelay": "0", "XLIGHT Disk slider-State": "2", "Position": "Default", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 AO-IOChannel": "Dev1/ao5", "520 AO-IOChannel": "Dev1/ao4", "528 AO-TriggerSequenceLength": "1000", "405 Shutter-MaxVolts": "5.0000", "470-DA Device": "470 Shutter", "PRIME-FTCapable": "No", "Core-ChannelGroup": "Channels", "555 AO-SupportsTriggering": "0", "PRIME-Y-dimension": "1200", "Frame": 0, "555 Shutter-Sequenceable": "1", "PRIME-Timing-ExposureTimeNs": "499998000.0000", "Core-ImageProcessor": "", "528-State": "0", "520 AO-Block voltage": "false", "XLIGHT Excitation Wheel-Label": "QUAD", "640 Shutter-Demo": "No", "COM3-StopBits": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "520 Shutter-IOChannel": "Dev1/ao11", "Width": 1200, "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-SupportsTriggering": "0", "640 AO-TriggerSampleRate": "100000", "520-Description": "DA device that is used as a shutter", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "405 Shutter-Sequenceable": "1", "405 Shutter-SupportsTriggering": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-TriggerSampleRate": "100000", "640 Shutter-MaxVolts": "5.0000", "555 AO-MaxVolts": "5.0000", "520 AO-TriggerSequenceLength": "1000", "COM3-BaudRate": "9600", "COM9-BaudRate": "9600", "Vincent-D3-Channel #": "Ch. #1", "PRIME-PMode": "Normal", "OpticalPath-Label": "Left(100)", "BitDepth": 16, "555 AO-TriggerSequenceLength": "1000", "445 Shutter-Description": "NI DAC", "405 AO-Sequenceable": "1", "PRIME-AcquisitionMethod": "Callbacks", "555 AO-Name": "AnalogIO", "PRIME-ShutterMode": "Pre-Exposure", "COM3-Handshaking": "Off", "445 AO-SupportsTriggering": "0", "ROI": "0-0-1200-1200", "COM9-Verbose": "1", "PRIME-Color": "OFF", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "PRIME-ShutterOpenDelay": "0", "COM1-Parity": "None", "Slice": 4, "SlicePosition": -10, "COM9-DelayBetweenCharsMs": "0.0000", "555-Name": "DA Shutter", "PIEZO-LudlSingleAxisName": "B", "XLIGHT Emission Wheel-Label": "679/29 (A)", "640 Shutter-Name": "AnalogIO", "640 AO-Sequenceable": "1", "520 Shutter-Demo": "No", "528 Shutter-Demo": "No", "470-Name": "DA Shutter", "520-DA Device": "520 Shutter", "XYStage-Description": "Ludl XY stage driver adapter", "445 Shutter-MinVolts": "0.0000", "528 AO-TriggerSampleRate": "100000", "405 AO-MinVolts": "0.0000", "405 Shutter-TriggerSequenceLength": "1000", "Height": 1200, "555 AO-Block voltage": "false", "640 Shutter-Volts": "5.0000", "PixelType": "GRAY16", "445 Shutter-Block voltage": "false", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "520 Shutter-TriggerSequenceLength": "1000", "COM9-Name": "COM9", "Core-SLM": "", "555 AO-Volts": "0.0000", "555 Shutter-Block voltage": "false", "XYStage-Speed": "1800.0000", "520 AO-MaxVolts": "5.0000", "528 Shutter-SupportsTriggering": "0", "COM1-Description": "Serial port driver (boost:asio)", "PRIME-MetadataEnabled": "No", "520 Shutter-MinVolts": "0.0000", "470 Shutter-Sequenceable": "1", "PRIME-PreampDelay": "0", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-MinVolts": "0.0000", "ChannelIndex": 2, "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Core-AutoShutter": "1", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "Exposure-ms": 320, "PRIME-PP 2 ENABLED": "Yes", "640 Shutter-TriggerSampleRate": "100000", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "470 Shutter-IOChannel": "Dev1/ao10", "405 AO-Demo": "No", "PixelSizeUm": 1.1, "470 AO-Demo": "No", "470 Shutter-MaxVolts": "5.0000", "Vincent-D3-Name": "Vincent-D3", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FileName": "img_000000000_Il-A_004.tif", "LudlController-Reset": "Operate", "PRIME-Exposure": "320.00", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "445-Name": "DA Shutter", "555 Shutter-Name": "AnalogIO", "520 Shutter-TriggerSampleRate": "100000", "445 Shutter-IOChannel": "Dev1/ao9", "PRIME-TransposeCorrection": "0", "XYStage-StepSize": "0.1000", "445-Description": "DA device that is used as a shutter", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 AO-IOChannel": "Dev1/ao7", "520 Shutter-Name": "AnalogIO", "COM3-DataBits": "8", "640 AO-Volts": "4.5000", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "555 Shutter-IOChannel": "Dev1/ao13", "WaitInterval": null, "520 Shutter-Sequenceable": "1", "YPositionUm": 31506.2, "445-State": "0", "445-DA Device": "445 Shutter", "PRIME-CircularBufferAutoSize": "ON", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "555 AO-TriggerSampleRate": "100000", "445 Shutter-Volts": "0.0000", "XLIGHT Hub-Port": "COM3", "Core-AutoFocus": "", "COM4-Handshaking": "Off", "528 Shutter-MinVolts": "0.0000", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "445 Shutter-Name": "AnalogIO", "528 Shutter-Sequenceable": "1", "XYStage-TransposeMirrorX": "0", "XYStage-TransposeMirrorY": "0", "PRIME-ReadoutRate": "100MHz 16bit", "XYStage-Name": "Stage", "640 AO-SupportsTriggering": "0", "640-Name": "DA Shutter", "640 Shutter-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "640 AO-Block voltage": "false", "445 Shutter-Sequenceable": "1", "470 AO-MaxVolts": "5.0000", "COM4-BaudRate": "9600", "470-Description": "DA device that is used as a shutter", "520 Shutter-Description": "NI DAC", "PRIME-CCDTemperature": "-14.8500", "Time": "2019-12-18 21:36:57 -0500", "640 AO-TriggerSequenceLength": "1000", "470 AO-Name": "AnalogIO", "XLIGHT Disk slider-Label": "Disk Pos 40um", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "Binning": "1x1", "TE2000-ModelType": "1", "COM3-AnswerTimeout": "500.0000", "555 Shutter-SupportsTriggering": "0", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "TE2000-Port": "COM4", "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Description": "NI DAC", "COM4-AnswerTimeout": "500.0000", "COM1-DelayBetweenCharsMs": "0.0000", "FilterBlock-Label": "Confocal", "445 AO-Name": "AnalogIO", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-Timing-ReadoutTimeNs": "24672000.0000", "528 AO-Demo": "No", "520 AO-Sequenceable": "1", "555-Description": "DA device that is used as a shutter", "405-DA Device": "405 Shutter", "405 AO-TriggerSequenceLength": "1000", "640 AO-MinVolts": "0.0000", "640 Shutter-Block voltage": "false", "FrameIndex": 0, "PRIME-Port": "CMOS", "555 AO-MinVolts": "0.0000", "COM3-Description": "Serial port driver (boost:asio)", "528 AO-Description": "NI DAC", "555 AO-IOChannel": "Dev1/ao6", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "555 AO-TriggerInputLine": "/Dev1/PFI0", "PRIME-ActualInterval-ms": "390.5200", "LudlController-Port": "COM9", "445 Shutter-Demo": "No", "PRIME-Trigger-Expose Out-Mux": "1", "SliceIndex": 4, "PRIME-CCDTemperatureSetPoint": "-15.0000", "405 Shutter-MinVolts": "0.0000", "PRIME-ExposeOutMode": "First Row", "AxisPositions": null, "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Volts": "0.0000", "528-Name": "DA Shutter", "555-State": "0", "XLIGHT Dichroic-Label": "Di03-R635-t1", "445 AO-Sequenceable": "1", "405-Name": "DA Shutter", "405 AO-IOChannel": "Dev1/ao0", "Core-Camera": "PRIME", "Focus-Name": "Focus", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "405 Shutter-IOChannel": "Dev1/ao8", "640 AO-Demo": "No", "640 Shutter-SupportsTriggering": "0", "445 AO-Demo": "No", "555-DA Device": "555 Shutter", "470 Shutter-Description": "NI DAC", "528 AO-Volts": "0.0000", "COM1-DataBits": "8", "Vincent-D3-Address": "x", "520 AO-SupportsTriggering": "0", "405-Description": "DA device that is used as a shutter", "640 Shutter-MinVolts": "0.0000", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "405 AO-Name": "AnalogIO", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "640 AO-TriggerInputLine": "/Dev1/PFI0", "405 AO-TriggerInputLine": "/Dev1/PFI0", "640-Description": "DA device that is used as a shutter", "445 Shutter-TriggerSampleRate": "100000", "470 Shutter-TriggerSequenceLength": "1000", "COM1-StopBits": "1", "520 Shutter-SupportsTriggering": "0", "405-State": "0", "Core-Initialize": "1", "XLIGHT Emission Wheel-State": "3", "520 AO-Description": "NI DAC", "528 AO-TriggerInputLine": "/Dev1/PFI0", "445 Shutter-MaxVolts": "5.0000", "COM9-Handshaking": "Off", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "XLIGHT Spin motor-Label": "On (spinning)", "PRIME-PP 5 ENABLED": "No", "UUID": "4bb595be-cd8c-4adf-b281-9c9a9dc52761", "PRIME-TriggerMode": "Internal Trigger", "COM1-Handshaking": "Off", "PRIME-Timing-PostTriggerDelayNs": "49344000.0000", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "Focus-Position": "17741.5000", "528 Shutter-Block voltage": "false", "Channel": "Il-A", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "555 Shutter-Demo": "No", "405 Shutter-Demo": "No", "528 AO-Name": "AnalogIO", "520-State": "0", "Core-Galvo": "", "405 Shutter-TriggerSampleRate": "100000", "Focus-FocusStepSize_(nm)": "100", "PRIME-PixelType": "16bit", "COM4-DataBits": "8", "470 AO-IOChannel": "Dev1/ao2", "520 AO-MinVolts": "0.0000", "COM9-Parity": "None", "PRIME-CircularBufferEnabled": "ON", "PRIME-PP 6 Reset": "No", "640 AO-Description": "NI DAC", "Source": "PRIME", "445 AO-MinVolts": "0.0000", "PIEZO-Name": "Stage", "445 AO-Description": "NI DAC", "PRIME-CircularBufferFrameCount": "46", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "520 AO-Volts": "0.0000", "528 Shutter-TriggerSampleRate": "100000", "XPositionUm": 19731.9, "OpticalPath-State": "4", "PRIME-PP 3 THRESHOLD": "80", "PRIME-PVCAM Version": "3.7.1", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Gain": "1-HDR", "640 Shutter-TriggerSequenceLength": "1000", "528 AO-SupportsTriggering": "0", "445 AO-TriggerInputLine": "/Dev1/PFI0", "OpticalPath-Name": "OpticalPath", "PRIME-PP 4 ENABLED": "Yes", "PRIME-PVCAM Adapter Version": "1.3.29", "COM3-Name": "COM3", "405 AO-MaxVolts": "5.0000", "520 Shutter-Block voltage": "false", "PRIME-Timing-ClearingTimeNs": "49344000.0000", "Core-Focus": "PIEZO", "528 AO-Block voltage": "false", "TE2000-Description": "Nikon TE2000 hub", "528 AO-MinVolts": "0.0000", "COM4-Parity": "None", "Summary": { "Slices": 11, "ChContrastMin": [ 0, 0, 0, 0, 0, 0 ], "PixelAspect": 1, "SlicesFirst": true, "Interval_ms": 0, "Time": "2019-12-18 21:36:42 -0500", "Prefix": "seq_4", "InitialPositionList": [ { "Label": "Pos0", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.21], "Focus": [17741.5], "XYStage": [ 44542.200000000004, 31636.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos1", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.22], "Focus": [17741.4], "XYStage": [ 43801.100000000006, 31637.9 ] }, "GridColumnIndex": 0 }, { "Label": "Pos2", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.5], "XYStage": [ 38561.3, 32040.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos3", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.185], "Focus": [17741.3], "XYStage": [ 37698.4, 32041.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos4", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.16], "Focus": [17741.5], "XYStage": [ 32375.4, 31758.9 ] }, "GridColumnIndex": 0 }, { "Label": "Pos5", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.17000000000002], "Focus": [17741.3], "XYStage": [ 31770.7, 31758.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos6", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.4], "XYStage": [ 26536.800000000003, 31068.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos7", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.205], "Focus": [17741.4], "XYStage": [ 25420.2, 31068.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos8", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.85], "Focus": [17741.3], "XYStage": [ 21059, 31508 ] }, "GridColumnIndex": 0 }, { "Label": "Pos9", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.82], "Focus": [17741.3], "XYStage": [ 19731.9, 31506.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos10", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.79], "Focus": [17741.4], "XYStage": [ 14528.5, 32007.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos11", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.83], "Focus": [17741.2], "XYStage": [ 13830.300000000001, 32009.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos12", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.79], "Focus": [17741.3], "XYStage": [ 8775.300000000001, 32017.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos13", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.815], "Focus": [17741.3], "XYStage": [ 8109.200000000001, 32008.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos14", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.805], "Focus": [17741.3], "XYStage": [ 3483.3, 31908.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos15", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.82], "Focus": [17741.2], "XYStage": [ 2686.8, 31903.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos16", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [211.48000000000002], "Focus": [17741.1], "XYStage": [ -1978.9, 31973.600000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos17", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [211.51500000000001], "Focus": [17741.1], "XYStage": [ -2957.8, 31982.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos18", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.195], "Focus": [17741.5], "XYStage": [ 47747.4, -576.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos19", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.23000000000002], "Focus": [17741.6], "XYStage": [ 47053.9, -576.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos20", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.205], "Focus": [17741.4], "XYStage": [ 41871.200000000004, -479.8 ] }, "GridColumnIndex": 0 }, { "Label": "Pos21", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.4], "XYStage": [ 40882.600000000006, -392 ] }, "GridColumnIndex": 0 }, { "Label": "Pos22", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.24], "Focus": [17741.4], "XYStage": [ 35905.6, -135.3 ] }, "GridColumnIndex": 0 }, { "Label": "Pos23", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.195], "Focus": [17741.5], "XYStage": [ 34990.9, -132.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos24", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.46], "Focus": [17741.4], "XYStage": [ 29678.100000000002, -958.1 ] }, "GridColumnIndex": 0 }, { "Label": "Pos25", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.44], "Focus": [17741.5], "XYStage": [ 28983.600000000002, -939.4000000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos26", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.475], "Focus": [17741.5], "XYStage": [ 23019.7, -122 ] }, "GridColumnIndex": 0 }, { "Label": "Pos27", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.44], "Focus": [17741.7], "XYStage": [ 22428, -116.80000000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos28", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.46], "Focus": [17741.7], "XYStage": [ 16364.900000000001, -181.10000000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos29", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.48000000000002], "Focus": [17741.5], "XYStage": [ 15712.1, -180 ] }, "GridColumnIndex": 0 }, { "Label": "Pos30", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.45000000000002], "Focus": [17741.7], "XYStage": [ 10346.1, 77.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos31", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.49], "Focus": [17741.4], "XYStage": [ 9755.5, 85.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos32", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.475], "Focus": [17741.5], "XYStage": [ 4775.5, 293.90000000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos33", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.445], "Focus": [17741.4], "XYStage": [ 3954.9, 293.90000000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos34", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.455], "Focus": [17741.4], "XYStage": [ -1324.9, 545.6 ] }, "GridColumnIndex": 0 }, { "Label": "Pos35", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.445], "Focus": [17741.3], "XYStage": [ -2097, 576.6 ] }, "GridColumnIndex": 0 } ], "z-step_um": 10, "GridRow": 0, "BitDepth": 16, "Depth": 2, "Channels": 6, "Source": "Micro-Manager", "ChColors": [ -16711681, -256, -65281, -1, -1, -16776961 ], "MetadataVersion": 10, "KeepShutterOpenChannels": true, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536, 65536 ], "CustomIntervals_ms": [], "Height": 1200, "MicroManagerVersion": "1.4.23 20180219", "GridColumn": 0, "UUID": "14a13bf7-c944-4672-b873-fcfbbb7ea13d", "PixelType": "GRAY16", "Width": 1200, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\Yan\\20191218-YC113-S7S8-miseq", "Frames": 1, "Comment": "", "UserName": "Nikon", "PixelSize_um": 1.1, "ROI": [ 0, 0, 1200, 1200 ], "Date": "2019-12-18", "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC", "P-DAPI-high" ], "KeepShutterOpenSlices": true, "IJType": 1, "ComputerName": "ZadorConfocal2", "Positions": 36, "PositionIndex": 9, "CameraTimeout": 20000, "TimeFirst": false }, "640 AO-Name": "AnalogIO", "Vincent-D3-Command": "Close" }, "FrameKey-0-2-5": { "COM4-StopBits": "1", "528 AO-MaxVolts": "5.0000", "XLIGHT Excitation Wheel-State": "1", "470 Shutter-Name": "AnalogIO", "Core-Shutter": "640", "555 AO-Demo": "No", "XLIGHT Dichroic-State": "2", "XYStage-StepSize-Y": "0.1000", "ZPositionUm": 248.82, "XYStage-StepSize-X": "0.1000", "PRIME-FirmwareVersion": "20.28", "PRIME-ClearCycles": "2", "520-Name": "DA Shutter", "405 Shutter-Name": "AnalogIO", "PIEZO-StepSize": "0.0050", "PRIME-ClearMode": "Pre-Exposure", "470 AO-MinVolts": "0.0000", "405 AO-SupportsTriggering": "1", "CameraChannelIndex": 0, "XYStage-StartSpeed": "500.0000", "COM9-StopBits": "2", "405 AO-Description": "NI DAC", "COM4-DelayBetweenCharsMs": "0.0000", "445 AO-Block voltage": "false", "520 AO-TriggerInputLine": "/Dev1/PFI0", "555 AO-Description": "NI DAC", "520 AO-TriggerSampleRate": "100000", "COM4-Verbose": "1", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM4-Name": "COM4", "405 Shutter-Volts": "0.0000", "Vincent-D3-Port": "COM1", "PRIME-PP 4 MIN ADU AFFECTED": "200", "470 Shutter-MinVolts": "0.0000", "PRIME-PP 3 ENABLED": "Yes", "470 AO-Description": "NI DAC", "PRIME-Description": "PVCAM API device adapter", "520 Shutter-MaxVolts": "5.0000", "520 Shutter-Volts": "0.0000", "528 AO-Sequenceable": "1", "PRIME-Binning": "1x1", "555 Shutter-MaxVolts": "5.0000", "528 Shutter-TriggerSequenceLength": "1000", "405 Shutter-Block voltage": "false", "XLIGHT Spin motor-State": "1", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "Vincent-D3-Time to open (ms)": "35.0000", "Vincent-D3-Time to close (ms)": "35.0000", "445 AO-TriggerSampleRate": "100000", "640 Shutter-IOChannel": "Dev1/ao14", "555 AO-Sequenceable": "1", "520 AO-Name": "AnalogIO", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "470 AO-Block voltage": "false", "COM1-Verbose": "1", "COM9-AnswerTimeout": "10000.0000", "528 Shutter-Name": "AnalogIO", "PRIME-Trigger Timeout (secs)": "10", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "NextFrame": 0, "470 AO-TriggerSequenceLength": "1000", "PRIME-TransposeXY": "0", "445 AO-TriggerSequenceLength": "1000", "COM1-Name": "COM1", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "COM9-DataBits": "8", "COM1-AnswerTimeout": "500.0000", "Camera": "", "TE2000-Version": "V2.50-V2.50-V2.10", "528 Shutter-Volts": "0.0000", "XLIGHT Hub-Name": "XLIGHT Hub", "PIEZO-Description": "Ludl stage driver adapter", "PositionName": "Pos9", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSampleRate": "100000", "PRIME-FanSpeedSetpoint": "High", "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "405 AO-TriggerSampleRate": "100000", "470 Shutter-Block voltage": "false", "470-State": "0", "COM3-Parity": "None", "405 AO-Volts": "0.0000", "PRIME-TransposeMirrorY": "0", "PRIME-ChipName": "GS144BSI", "470 AO-Volts": "0.0000", "PRIME-TransposeMirrorX": "0", "445 Shutter-SupportsTriggering": "0", "470 Shutter-SupportsTriggering": "0", "XLIGHT Hub-Description": "XLIGHT hub", "PRIME-PreampOffLimit": "10000", "470 AO-Sequenceable": "1", "528 Shutter-MaxVolts": "5.0000", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "Core-XYStage": "XYStage", "470 AO-TriggerSampleRate": "100000", "PRIME-Timing-PreTriggerDelayNs": "24672000.0000", "528-DA Device": "528 Shutter", "Focus-Description": "Nikon TE2000 focus adapter", "FilterBlock-State": "5", "520 AO-Demo": "No", "COM3-DelayBetweenCharsMs": "0.0000", "470 Shutter-Volts": "0.0000", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "PRIME-PP 2 THRESHOLD": "125", "PRIME-PP 4 THRESHOLD": "75", "XYStage-Acceleration": "4.0000", "470 Shutter-Demo": "No", "PRIME-Name": "PMPCIECam00", "640-DA Device": "640 Shutter", "405 AO-Block voltage": "false", "ElapsedTime-ms": 389759, "PRIME-Offset": "100", "445 AO-IOChannel": "Dev1/ao1", "640 AO-MaxVolts": "5.0000", "PositionIndex": 9, "PRIME-PP 1 ENABLED": "Yes", "COM1-BaudRate": "9600", "640-State": "1", "COM3-Verbose": "1", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "LudlController-TransmissionDelay": "0", "PRIME-FullWellCapacity": "62000", "PRIME-SMARTStreamingEnabled": "No", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "640 Shutter-Description": "NI DAC", "528-Description": "DA device that is used as a shutter", "COM4-Description": "Serial port driver (boost:asio)", "405 Shutter-Description": "NI DAC", "TE2000-Name": "TE2000", "528 Shutter-Description": "NI DAC", "COM9-Description": "Serial port driver (boost:asio)", "LudlController-Name": "LudlController", "445 Shutter-TriggerSequenceLength": "1000", "PRIME-SerialNumber": "A18B203008", "445 AO-Volts": "0.0000", "PRIME-X-dimension": "1200", "PRIME-ShutterCloseDelay": "0", "XLIGHT Disk slider-State": "2", "Position": "Default", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 AO-IOChannel": "Dev1/ao5", "520 AO-IOChannel": "Dev1/ao4", "528 AO-TriggerSequenceLength": "1000", "405 Shutter-MaxVolts": "5.0000", "470-DA Device": "470 Shutter", "PRIME-FTCapable": "No", "Core-ChannelGroup": "Channels", "555 AO-SupportsTriggering": "0", "PRIME-Y-dimension": "1200", "Frame": 0, "555 Shutter-Sequenceable": "1", "PRIME-Timing-ExposureTimeNs": "499998000.0000", "Core-ImageProcessor": "", "528-State": "0", "520 AO-Block voltage": "false", "XLIGHT Excitation Wheel-Label": "QUAD", "640 Shutter-Demo": "No", "COM3-StopBits": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "520 Shutter-IOChannel": "Dev1/ao11", "Width": 1200, "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-SupportsTriggering": "0", "640 AO-TriggerSampleRate": "100000", "520-Description": "DA device that is used as a shutter", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "405 Shutter-Sequenceable": "1", "405 Shutter-SupportsTriggering": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-TriggerSampleRate": "100000", "640 Shutter-MaxVolts": "5.0000", "555 AO-MaxVolts": "5.0000", "520 AO-TriggerSequenceLength": "1000", "COM3-BaudRate": "9600", "COM9-BaudRate": "9600", "Vincent-D3-Channel #": "Ch. #1", "PRIME-PMode": "Normal", "OpticalPath-Label": "Left(100)", "BitDepth": 16, "555 AO-TriggerSequenceLength": "1000", "445 Shutter-Description": "NI DAC", "405 AO-Sequenceable": "1", "PRIME-AcquisitionMethod": "Callbacks", "555 AO-Name": "AnalogIO", "PRIME-ShutterMode": "Pre-Exposure", "COM3-Handshaking": "Off", "445 AO-SupportsTriggering": "0", "ROI": "0-0-1200-1200", "COM9-Verbose": "1", "PRIME-Color": "OFF", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "PRIME-ShutterOpenDelay": "0", "COM1-Parity": "None", "Slice": 5, "SlicePosition": 0, "COM9-DelayBetweenCharsMs": "0.0000", "555-Name": "DA Shutter", "PIEZO-LudlSingleAxisName": "B", "XLIGHT Emission Wheel-Label": "679/29 (A)", "640 Shutter-Name": "AnalogIO", "640 AO-Sequenceable": "1", "520 Shutter-Demo": "No", "528 Shutter-Demo": "No", "470-Name": "DA Shutter", "520-DA Device": "520 Shutter", "XYStage-Description": "Ludl XY stage driver adapter", "445 Shutter-MinVolts": "0.0000", "528 AO-TriggerSampleRate": "100000", "405 AO-MinVolts": "0.0000", "405 Shutter-TriggerSequenceLength": "1000", "Height": 1200, "555 AO-Block voltage": "false", "640 Shutter-Volts": "5.0000", "PixelType": "GRAY16", "445 Shutter-Block voltage": "false", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "520 Shutter-TriggerSequenceLength": "1000", "COM9-Name": "COM9", "Core-SLM": "", "555 AO-Volts": "0.0000", "555 Shutter-Block voltage": "false", "XYStage-Speed": "1800.0000", "520 AO-MaxVolts": "5.0000", "528 Shutter-SupportsTriggering": "0", "COM1-Description": "Serial port driver (boost:asio)", "PRIME-MetadataEnabled": "No", "520 Shutter-MinVolts": "0.0000", "470 Shutter-Sequenceable": "1", "PRIME-PreampDelay": "0", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-MinVolts": "0.0000", "ChannelIndex": 2, "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Core-AutoShutter": "1", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "Exposure-ms": 320, "PRIME-PP 2 ENABLED": "Yes", "640 Shutter-TriggerSampleRate": "100000", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "470 Shutter-IOChannel": "Dev1/ao10", "405 AO-Demo": "No", "PixelSizeUm": 1.1, "470 AO-Demo": "No", "470 Shutter-MaxVolts": "5.0000", "Vincent-D3-Name": "Vincent-D3", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FileName": "img_000000000_Il-A_005.tif", "LudlController-Reset": "Operate", "PRIME-Exposure": "320.00", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "445-Name": "DA Shutter", "555 Shutter-Name": "AnalogIO", "520 Shutter-TriggerSampleRate": "100000", "445 Shutter-IOChannel": "Dev1/ao9", "PRIME-TransposeCorrection": "0", "XYStage-StepSize": "0.1000", "445-Description": "DA device that is used as a shutter", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 AO-IOChannel": "Dev1/ao7", "520 Shutter-Name": "AnalogIO", "COM3-DataBits": "8", "640 AO-Volts": "4.5000", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "555 Shutter-IOChannel": "Dev1/ao13", "WaitInterval": null, "520 Shutter-Sequenceable": "1", "YPositionUm": 31506.2, "445-State": "0", "445-DA Device": "445 Shutter", "PRIME-CircularBufferAutoSize": "ON", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "555 AO-TriggerSampleRate": "100000", "445 Shutter-Volts": "0.0000", "XLIGHT Hub-Port": "COM3", "Core-AutoFocus": "", "COM4-Handshaking": "Off", "528 Shutter-MinVolts": "0.0000", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "445 Shutter-Name": "AnalogIO", "528 Shutter-Sequenceable": "1", "XYStage-TransposeMirrorX": "0", "XYStage-TransposeMirrorY": "0", "PRIME-ReadoutRate": "100MHz 16bit", "XYStage-Name": "Stage", "640 AO-SupportsTriggering": "0", "640-Name": "DA Shutter", "640 Shutter-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "640 AO-Block voltage": "false", "445 Shutter-Sequenceable": "1", "470 AO-MaxVolts": "5.0000", "COM4-BaudRate": "9600", "470-Description": "DA device that is used as a shutter", "520 Shutter-Description": "NI DAC", "PRIME-CCDTemperature": "-14.8500", "Time": "2019-12-18 21:36:57 -0500", "640 AO-TriggerSequenceLength": "1000", "470 AO-Name": "AnalogIO", "XLIGHT Disk slider-Label": "Disk Pos 40um", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "Binning": "1x1", "TE2000-ModelType": "1", "COM3-AnswerTimeout": "500.0000", "555 Shutter-SupportsTriggering": "0", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "TE2000-Port": "COM4", "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Description": "NI DAC", "COM4-AnswerTimeout": "500.0000", "COM1-DelayBetweenCharsMs": "0.0000", "FilterBlock-Label": "Confocal", "445 AO-Name": "AnalogIO", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-Timing-ReadoutTimeNs": "24672000.0000", "528 AO-Demo": "No", "520 AO-Sequenceable": "1", "555-Description": "DA device that is used as a shutter", "405-DA Device": "405 Shutter", "405 AO-TriggerSequenceLength": "1000", "640 AO-MinVolts": "0.0000", "640 Shutter-Block voltage": "false", "FrameIndex": 0, "PRIME-Port": "CMOS", "555 AO-MinVolts": "0.0000", "COM3-Description": "Serial port driver (boost:asio)", "528 AO-Description": "NI DAC", "555 AO-IOChannel": "Dev1/ao6", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "555 AO-TriggerInputLine": "/Dev1/PFI0", "PRIME-ActualInterval-ms": "390.5200", "LudlController-Port": "COM9", "445 Shutter-Demo": "No", "PRIME-Trigger-Expose Out-Mux": "1", "SliceIndex": 5, "PRIME-CCDTemperatureSetPoint": "-15.0000", "405 Shutter-MinVolts": "0.0000", "PRIME-ExposeOutMode": "First Row", "AxisPositions": null, "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Volts": "0.0000", "528-Name": "DA Shutter", "555-State": "0", "XLIGHT Dichroic-Label": "Di03-R635-t1", "445 AO-Sequenceable": "1", "405-Name": "DA Shutter", "405 AO-IOChannel": "Dev1/ao0", "Core-Camera": "PRIME", "Focus-Name": "Focus", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "405 Shutter-IOChannel": "Dev1/ao8", "640 AO-Demo": "No", "640 Shutter-SupportsTriggering": "0", "445 AO-Demo": "No", "555-DA Device": "555 Shutter", "470 Shutter-Description": "NI DAC", "528 AO-Volts": "0.0000", "COM1-DataBits": "8", "Vincent-D3-Address": "x", "520 AO-SupportsTriggering": "0", "405-Description": "DA device that is used as a shutter", "640 Shutter-MinVolts": "0.0000", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "405 AO-Name": "AnalogIO", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "640 AO-TriggerInputLine": "/Dev1/PFI0", "405 AO-TriggerInputLine": "/Dev1/PFI0", "640-Description": "DA device that is used as a shutter", "445 Shutter-TriggerSampleRate": "100000", "470 Shutter-TriggerSequenceLength": "1000", "COM1-StopBits": "1", "520 Shutter-SupportsTriggering": "0", "405-State": "0", "Core-Initialize": "1", "XLIGHT Emission Wheel-State": "3", "520 AO-Description": "NI DAC", "528 AO-TriggerInputLine": "/Dev1/PFI0", "445 Shutter-MaxVolts": "5.0000", "COM9-Handshaking": "Off", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "XLIGHT Spin motor-Label": "On (spinning)", "PRIME-PP 5 ENABLED": "No", "UUID": "90fb178a-d40c-40aa-bfa4-6675b1ef9904", "PRIME-TriggerMode": "Internal Trigger", "COM1-Handshaking": "Off", "PRIME-Timing-PostTriggerDelayNs": "49344000.0000", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "Focus-Position": "17741.5000", "528 Shutter-Block voltage": "false", "Channel": "Il-A", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "555 Shutter-Demo": "No", "405 Shutter-Demo": "No", "528 AO-Name": "AnalogIO", "520-State": "0", "Core-Galvo": "", "405 Shutter-TriggerSampleRate": "100000", "Focus-FocusStepSize_(nm)": "100", "PRIME-PixelType": "16bit", "COM4-DataBits": "8", "470 AO-IOChannel": "Dev1/ao2", "520 AO-MinVolts": "0.0000", "COM9-Parity": "None", "PRIME-CircularBufferEnabled": "ON", "PRIME-PP 6 Reset": "No", "640 AO-Description": "NI DAC", "Source": "PRIME", "445 AO-MinVolts": "0.0000", "PIEZO-Name": "Stage", "445 AO-Description": "NI DAC", "PRIME-CircularBufferFrameCount": "46", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "520 AO-Volts": "0.0000", "528 Shutter-TriggerSampleRate": "100000", "XPositionUm": 19731.9, "OpticalPath-State": "4", "PRIME-PP 3 THRESHOLD": "80", "PRIME-PVCAM Version": "3.7.1", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Gain": "1-HDR", "640 Shutter-TriggerSequenceLength": "1000", "528 AO-SupportsTriggering": "0", "445 AO-TriggerInputLine": "/Dev1/PFI0", "OpticalPath-Name": "OpticalPath", "PRIME-PP 4 ENABLED": "Yes", "PRIME-PVCAM Adapter Version": "1.3.29", "COM3-Name": "COM3", "405 AO-MaxVolts": "5.0000", "520 Shutter-Block voltage": "false", "PRIME-Timing-ClearingTimeNs": "49344000.0000", "Core-Focus": "PIEZO", "528 AO-Block voltage": "false", "TE2000-Description": "Nikon TE2000 hub", "528 AO-MinVolts": "0.0000", "COM4-Parity": "None", "Summary": { "Slices": 11, "ChContrastMin": [ 0, 0, 0, 0, 0, 0 ], "PixelAspect": 1, "SlicesFirst": true, "Interval_ms": 0, "Time": "2019-12-18 21:36:42 -0500", "Prefix": "seq_4", "InitialPositionList": [ { "Label": "Pos0", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.21], "Focus": [17741.5], "XYStage": [ 44542.200000000004, 31636.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos1", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.22], "Focus": [17741.4], "XYStage": [ 43801.100000000006, 31637.9 ] }, "GridColumnIndex": 0 }, { "Label": "Pos2", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.5], "XYStage": [ 38561.3, 32040.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos3", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.185], "Focus": [17741.3], "XYStage": [ 37698.4, 32041.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos4", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.16], "Focus": [17741.5], "XYStage": [ 32375.4, 31758.9 ] }, "GridColumnIndex": 0 }, { "Label": "Pos5", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.17000000000002], "Focus": [17741.3], "XYStage": [ 31770.7, 31758.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos6", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.4], "XYStage": [ 26536.800000000003, 31068.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos7", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.205], "Focus": [17741.4], "XYStage": [ 25420.2, 31068.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos8", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.85], "Focus": [17741.3], "XYStage": [ 21059, 31508 ] }, "GridColumnIndex": 0 }, { "Label": "Pos9", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.82], "Focus": [17741.3], "XYStage": [ 19731.9, 31506.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos10", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.79], "Focus": [17741.4], "XYStage": [ 14528.5, 32007.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos11", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.83], "Focus": [17741.2], "XYStage": [ 13830.300000000001, 32009.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos12", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.79], "Focus": [17741.3], "XYStage": [ 8775.300000000001, 32017.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos13", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.815], "Focus": [17741.3], "XYStage": [ 8109.200000000001, 32008.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos14", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.805], "Focus": [17741.3], "XYStage": [ 3483.3, 31908.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos15", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.82], "Focus": [17741.2], "XYStage": [ 2686.8, 31903.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos16", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [211.48000000000002], "Focus": [17741.1], "XYStage": [ -1978.9, 31973.600000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos17", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [211.51500000000001], "Focus": [17741.1], "XYStage": [ -2957.8, 31982.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos18", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.195], "Focus": [17741.5], "XYStage": [ 47747.4, -576.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos19", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.23000000000002], "Focus": [17741.6], "XYStage": [ 47053.9, -576.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos20", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.205], "Focus": [17741.4], "XYStage": [ 41871.200000000004, -479.8 ] }, "GridColumnIndex": 0 }, { "Label": "Pos21", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.4], "XYStage": [ 40882.600000000006, -392 ] }, "GridColumnIndex": 0 }, { "Label": "Pos22", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.24], "Focus": [17741.4], "XYStage": [ 35905.6, -135.3 ] }, "GridColumnIndex": 0 }, { "Label": "Pos23", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.195], "Focus": [17741.5], "XYStage": [ 34990.9, -132.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos24", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.46], "Focus": [17741.4], "XYStage": [ 29678.100000000002, -958.1 ] }, "GridColumnIndex": 0 }, { "Label": "Pos25", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.44], "Focus": [17741.5], "XYStage": [ 28983.600000000002, -939.4000000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos26", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.475], "Focus": [17741.5], "XYStage": [ 23019.7, -122 ] }, "GridColumnIndex": 0 }, { "Label": "Pos27", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.44], "Focus": [17741.7], "XYStage": [ 22428, -116.80000000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos28", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.46], "Focus": [17741.7], "XYStage": [ 16364.900000000001, -181.10000000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos29", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.48000000000002], "Focus": [17741.5], "XYStage": [ 15712.1, -180 ] }, "GridColumnIndex": 0 }, { "Label": "Pos30", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.45000000000002], "Focus": [17741.7], "XYStage": [ 10346.1, 77.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos31", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.49], "Focus": [17741.4], "XYStage": [ 9755.5, 85.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos32", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.475], "Focus": [17741.5], "XYStage": [ 4775.5, 293.90000000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos33", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.445], "Focus": [17741.4], "XYStage": [ 3954.9, 293.90000000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos34", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.455], "Focus": [17741.4], "XYStage": [ -1324.9, 545.6 ] }, "GridColumnIndex": 0 }, { "Label": "Pos35", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.445], "Focus": [17741.3], "XYStage": [ -2097, 576.6 ] }, "GridColumnIndex": 0 } ], "z-step_um": 10, "GridRow": 0, "BitDepth": 16, "Depth": 2, "Channels": 6, "Source": "Micro-Manager", "ChColors": [ -16711681, -256, -65281, -1, -1, -16776961 ], "MetadataVersion": 10, "KeepShutterOpenChannels": true, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536, 65536 ], "CustomIntervals_ms": [], "Height": 1200, "MicroManagerVersion": "1.4.23 20180219", "GridColumn": 0, "UUID": "14a13bf7-c944-4672-b873-fcfbbb7ea13d", "PixelType": "GRAY16", "Width": 1200, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\Yan\\20191218-YC113-S7S8-miseq", "Frames": 1, "Comment": "", "UserName": "Nikon", "PixelSize_um": 1.1, "ROI": [ 0, 0, 1200, 1200 ], "Date": "2019-12-18", "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC", "P-DAPI-high" ], "KeepShutterOpenSlices": true, "IJType": 1, "ComputerName": "ZadorConfocal2", "Positions": 36, "PositionIndex": 9, "CameraTimeout": 20000, "TimeFirst": false }, "640 AO-Name": "AnalogIO", "Vincent-D3-Command": "Close" }, "FrameKey-0-2-6": { "COM4-StopBits": "1", "528 AO-MaxVolts": "5.0000", "XLIGHT Excitation Wheel-State": "1", "470 Shutter-Name": "AnalogIO", "Core-Shutter": "640", "555 AO-Demo": "No", "XLIGHT Dichroic-State": "2", "XYStage-StepSize-Y": "0.1000", "ZPositionUm": 258.82, "XYStage-StepSize-X": "0.1000", "PRIME-FirmwareVersion": "20.28", "PRIME-ClearCycles": "2", "520-Name": "DA Shutter", "405 Shutter-Name": "AnalogIO", "PIEZO-StepSize": "0.0050", "PRIME-ClearMode": "Pre-Exposure", "470 AO-MinVolts": "0.0000", "405 AO-SupportsTriggering": "1", "CameraChannelIndex": 0, "XYStage-StartSpeed": "500.0000", "COM9-StopBits": "2", "405 AO-Description": "NI DAC", "COM4-DelayBetweenCharsMs": "0.0000", "445 AO-Block voltage": "false", "520 AO-TriggerInputLine": "/Dev1/PFI0", "555 AO-Description": "NI DAC", "520 AO-TriggerSampleRate": "100000", "COM4-Verbose": "1", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM4-Name": "COM4", "405 Shutter-Volts": "0.0000", "Vincent-D3-Port": "COM1", "PRIME-PP 4 MIN ADU AFFECTED": "200", "470 Shutter-MinVolts": "0.0000", "PRIME-PP 3 ENABLED": "Yes", "470 AO-Description": "NI DAC", "PRIME-Description": "PVCAM API device adapter", "520 Shutter-MaxVolts": "5.0000", "520 Shutter-Volts": "0.0000", "528 AO-Sequenceable": "1", "PRIME-Binning": "1x1", "555 Shutter-MaxVolts": "5.0000", "528 Shutter-TriggerSequenceLength": "1000", "405 Shutter-Block voltage": "false", "XLIGHT Spin motor-State": "1", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "Vincent-D3-Time to open (ms)": "35.0000", "Vincent-D3-Time to close (ms)": "35.0000", "445 AO-TriggerSampleRate": "100000", "640 Shutter-IOChannel": "Dev1/ao14", "555 AO-Sequenceable": "1", "520 AO-Name": "AnalogIO", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "470 AO-Block voltage": "false", "COM1-Verbose": "1", "COM9-AnswerTimeout": "10000.0000", "528 Shutter-Name": "AnalogIO", "PRIME-Trigger Timeout (secs)": "10", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "NextFrame": 0, "470 AO-TriggerSequenceLength": "1000", "PRIME-TransposeXY": "0", "445 AO-TriggerSequenceLength": "1000", "COM1-Name": "COM1", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "COM9-DataBits": "8", "COM1-AnswerTimeout": "500.0000", "Camera": "", "TE2000-Version": "V2.50-V2.50-V2.10", "528 Shutter-Volts": "0.0000", "XLIGHT Hub-Name": "XLIGHT Hub", "PIEZO-Description": "Ludl stage driver adapter", "PositionName": "Pos9", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSampleRate": "100000", "PRIME-FanSpeedSetpoint": "High", "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "405 AO-TriggerSampleRate": "100000", "470 Shutter-Block voltage": "false", "470-State": "0", "COM3-Parity": "None", "405 AO-Volts": "0.0000", "PRIME-TransposeMirrorY": "0", "PRIME-ChipName": "GS144BSI", "470 AO-Volts": "0.0000", "PRIME-TransposeMirrorX": "0", "445 Shutter-SupportsTriggering": "0", "470 Shutter-SupportsTriggering": "0", "XLIGHT Hub-Description": "XLIGHT hub", "PRIME-PreampOffLimit": "10000", "470 AO-Sequenceable": "1", "528 Shutter-MaxVolts": "5.0000", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "Core-XYStage": "XYStage", "470 AO-TriggerSampleRate": "100000", "PRIME-Timing-PreTriggerDelayNs": "24672000.0000", "528-DA Device": "528 Shutter", "Focus-Description": "Nikon TE2000 focus adapter", "FilterBlock-State": "5", "520 AO-Demo": "No", "COM3-DelayBetweenCharsMs": "0.0000", "470 Shutter-Volts": "0.0000", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "PRIME-PP 2 THRESHOLD": "125", "PRIME-PP 4 THRESHOLD": "75", "XYStage-Acceleration": "4.0000", "470 Shutter-Demo": "No", "PRIME-Name": "PMPCIECam00", "640-DA Device": "640 Shutter", "405 AO-Block voltage": "false", "ElapsedTime-ms": 390321, "PRIME-Offset": "100", "445 AO-IOChannel": "Dev1/ao1", "640 AO-MaxVolts": "5.0000", "PositionIndex": 9, "PRIME-PP 1 ENABLED": "Yes", "COM1-BaudRate": "9600", "640-State": "1", "COM3-Verbose": "1", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "LudlController-TransmissionDelay": "0", "PRIME-FullWellCapacity": "62000", "PRIME-SMARTStreamingEnabled": "No", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "640 Shutter-Description": "NI DAC", "528-Description": "DA device that is used as a shutter", "COM4-Description": "Serial port driver (boost:asio)", "405 Shutter-Description": "NI DAC", "TE2000-Name": "TE2000", "528 Shutter-Description": "NI DAC", "COM9-Description": "Serial port driver (boost:asio)", "LudlController-Name": "LudlController", "445 Shutter-TriggerSequenceLength": "1000", "PRIME-SerialNumber": "A18B203008", "445 AO-Volts": "0.0000", "PRIME-X-dimension": "1200", "PRIME-ShutterCloseDelay": "0", "XLIGHT Disk slider-State": "2", "Position": "Default", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 AO-IOChannel": "Dev1/ao5", "520 AO-IOChannel": "Dev1/ao4", "528 AO-TriggerSequenceLength": "1000", "405 Shutter-MaxVolts": "5.0000", "470-DA Device": "470 Shutter", "PRIME-FTCapable": "No", "Core-ChannelGroup": "Channels", "555 AO-SupportsTriggering": "0", "PRIME-Y-dimension": "1200", "Frame": 0, "555 Shutter-Sequenceable": "1", "PRIME-Timing-ExposureTimeNs": "499998000.0000", "Core-ImageProcessor": "", "528-State": "0", "520 AO-Block voltage": "false", "XLIGHT Excitation Wheel-Label": "QUAD", "640 Shutter-Demo": "No", "COM3-StopBits": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "520 Shutter-IOChannel": "Dev1/ao11", "Width": 1200, "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-SupportsTriggering": "0", "640 AO-TriggerSampleRate": "100000", "520-Description": "DA device that is used as a shutter", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "405 Shutter-Sequenceable": "1", "405 Shutter-SupportsTriggering": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-TriggerSampleRate": "100000", "640 Shutter-MaxVolts": "5.0000", "555 AO-MaxVolts": "5.0000", "520 AO-TriggerSequenceLength": "1000", "COM3-BaudRate": "9600", "COM9-BaudRate": "9600", "Vincent-D3-Channel #": "Ch. #1", "PRIME-PMode": "Normal", "OpticalPath-Label": "Left(100)", "BitDepth": 16, "555 AO-TriggerSequenceLength": "1000", "445 Shutter-Description": "NI DAC", "405 AO-Sequenceable": "1", "PRIME-AcquisitionMethod": "Callbacks", "555 AO-Name": "AnalogIO", "PRIME-ShutterMode": "Pre-Exposure", "COM3-Handshaking": "Off", "445 AO-SupportsTriggering": "0", "ROI": "0-0-1200-1200", "COM9-Verbose": "1", "PRIME-Color": "OFF", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "PRIME-ShutterOpenDelay": "0", "COM1-Parity": "None", "Slice": 6, "SlicePosition": 10, "COM9-DelayBetweenCharsMs": "0.0000", "555-Name": "DA Shutter", "PIEZO-LudlSingleAxisName": "B", "XLIGHT Emission Wheel-Label": "679/29 (A)", "640 Shutter-Name": "AnalogIO", "640 AO-Sequenceable": "1", "520 Shutter-Demo": "No", "528 Shutter-Demo": "No", "470-Name": "DA Shutter", "520-DA Device": "520 Shutter", "XYStage-Description": "Ludl XY stage driver adapter", "445 Shutter-MinVolts": "0.0000", "528 AO-TriggerSampleRate": "100000", "405 AO-MinVolts": "0.0000", "405 Shutter-TriggerSequenceLength": "1000", "Height": 1200, "555 AO-Block voltage": "false", "640 Shutter-Volts": "5.0000", "PixelType": "GRAY16", "445 Shutter-Block voltage": "false", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "520 Shutter-TriggerSequenceLength": "1000", "COM9-Name": "COM9", "Core-SLM": "", "555 AO-Volts": "0.0000", "555 Shutter-Block voltage": "false", "XYStage-Speed": "1800.0000", "520 AO-MaxVolts": "5.0000", "528 Shutter-SupportsTriggering": "0", "COM1-Description": "Serial port driver (boost:asio)", "PRIME-MetadataEnabled": "No", "520 Shutter-MinVolts": "0.0000", "470 Shutter-Sequenceable": "1", "PRIME-PreampDelay": "0", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-MinVolts": "0.0000", "ChannelIndex": 2, "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Core-AutoShutter": "1", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "Exposure-ms": 320, "PRIME-PP 2 ENABLED": "Yes", "640 Shutter-TriggerSampleRate": "100000", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "470 Shutter-IOChannel": "Dev1/ao10", "405 AO-Demo": "No", "PixelSizeUm": 1.1, "470 AO-Demo": "No", "470 Shutter-MaxVolts": "5.0000", "Vincent-D3-Name": "Vincent-D3", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FileName": "img_000000000_Il-A_006.tif", "LudlController-Reset": "Operate", "PRIME-Exposure": "320.00", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "445-Name": "DA Shutter", "555 Shutter-Name": "AnalogIO", "520 Shutter-TriggerSampleRate": "100000", "445 Shutter-IOChannel": "Dev1/ao9", "PRIME-TransposeCorrection": "0", "XYStage-StepSize": "0.1000", "445-Description": "DA device that is used as a shutter", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 AO-IOChannel": "Dev1/ao7", "520 Shutter-Name": "AnalogIO", "COM3-DataBits": "8", "640 AO-Volts": "4.5000", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "555 Shutter-IOChannel": "Dev1/ao13", "WaitInterval": null, "520 Shutter-Sequenceable": "1", "YPositionUm": 31506.2, "445-State": "0", "445-DA Device": "445 Shutter", "PRIME-CircularBufferAutoSize": "ON", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "555 AO-TriggerSampleRate": "100000", "445 Shutter-Volts": "0.0000", "XLIGHT Hub-Port": "COM3", "Core-AutoFocus": "", "COM4-Handshaking": "Off", "528 Shutter-MinVolts": "0.0000", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "445 Shutter-Name": "AnalogIO", "528 Shutter-Sequenceable": "1", "XYStage-TransposeMirrorX": "0", "XYStage-TransposeMirrorY": "0", "PRIME-ReadoutRate": "100MHz 16bit", "XYStage-Name": "Stage", "640 AO-SupportsTriggering": "0", "640-Name": "DA Shutter", "640 Shutter-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "640 AO-Block voltage": "false", "445 Shutter-Sequenceable": "1", "470 AO-MaxVolts": "5.0000", "COM4-BaudRate": "9600", "470-Description": "DA device that is used as a shutter", "520 Shutter-Description": "NI DAC", "PRIME-CCDTemperature": "-14.8500", "Time": "2019-12-18 21:36:58 -0500", "640 AO-TriggerSequenceLength": "1000", "470 AO-Name": "AnalogIO", "XLIGHT Disk slider-Label": "Disk Pos 40um", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "Binning": "1x1", "TE2000-ModelType": "1", "COM3-AnswerTimeout": "500.0000", "555 Shutter-SupportsTriggering": "0", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "TE2000-Port": "COM4", "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Description": "NI DAC", "COM4-AnswerTimeout": "500.0000", "COM1-DelayBetweenCharsMs": "0.0000", "FilterBlock-Label": "Confocal", "445 AO-Name": "AnalogIO", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-Timing-ReadoutTimeNs": "24672000.0000", "528 AO-Demo": "No", "520 AO-Sequenceable": "1", "555-Description": "DA device that is used as a shutter", "405-DA Device": "405 Shutter", "405 AO-TriggerSequenceLength": "1000", "640 AO-MinVolts": "0.0000", "640 Shutter-Block voltage": "false", "FrameIndex": 0, "PRIME-Port": "CMOS", "555 AO-MinVolts": "0.0000", "COM3-Description": "Serial port driver (boost:asio)", "528 AO-Description": "NI DAC", "555 AO-IOChannel": "Dev1/ao6", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "555 AO-TriggerInputLine": "/Dev1/PFI0", "PRIME-ActualInterval-ms": "390.5200", "LudlController-Port": "COM9", "445 Shutter-Demo": "No", "PRIME-Trigger-Expose Out-Mux": "1", "SliceIndex": 6, "PRIME-CCDTemperatureSetPoint": "-15.0000", "405 Shutter-MinVolts": "0.0000", "PRIME-ExposeOutMode": "First Row", "AxisPositions": null, "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Volts": "0.0000", "528-Name": "DA Shutter", "555-State": "0", "XLIGHT Dichroic-Label": "Di03-R635-t1", "445 AO-Sequenceable": "1", "405-Name": "DA Shutter", "405 AO-IOChannel": "Dev1/ao0", "Core-Camera": "PRIME", "Focus-Name": "Focus", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "405 Shutter-IOChannel": "Dev1/ao8", "640 AO-Demo": "No", "640 Shutter-SupportsTriggering": "0", "445 AO-Demo": "No", "555-DA Device": "555 Shutter", "470 Shutter-Description": "NI DAC", "528 AO-Volts": "0.0000", "COM1-DataBits": "8", "Vincent-D3-Address": "x", "520 AO-SupportsTriggering": "0", "405-Description": "DA device that is used as a shutter", "640 Shutter-MinVolts": "0.0000", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "405 AO-Name": "AnalogIO", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "640 AO-TriggerInputLine": "/Dev1/PFI0", "405 AO-TriggerInputLine": "/Dev1/PFI0", "640-Description": "DA device that is used as a shutter", "445 Shutter-TriggerSampleRate": "100000", "470 Shutter-TriggerSequenceLength": "1000", "COM1-StopBits": "1", "520 Shutter-SupportsTriggering": "0", "405-State": "0", "Core-Initialize": "1", "XLIGHT Emission Wheel-State": "3", "520 AO-Description": "NI DAC", "528 AO-TriggerInputLine": "/Dev1/PFI0", "445 Shutter-MaxVolts": "5.0000", "COM9-Handshaking": "Off", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "XLIGHT Spin motor-Label": "On (spinning)", "PRIME-PP 5 ENABLED": "No", "UUID": "e111cf27-40a4-42c9-923d-86b3675f5645", "PRIME-TriggerMode": "Internal Trigger", "COM1-Handshaking": "Off", "PRIME-Timing-PostTriggerDelayNs": "49344000.0000", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "Focus-Position": "17741.5000", "528 Shutter-Block voltage": "false", "Channel": "Il-A", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "555 Shutter-Demo": "No", "405 Shutter-Demo": "No", "528 AO-Name": "AnalogIO", "520-State": "0", "Core-Galvo": "", "405 Shutter-TriggerSampleRate": "100000", "Focus-FocusStepSize_(nm)": "100", "PRIME-PixelType": "16bit", "COM4-DataBits": "8", "470 AO-IOChannel": "Dev1/ao2", "520 AO-MinVolts": "0.0000", "COM9-Parity": "None", "PRIME-CircularBufferEnabled": "ON", "PRIME-PP 6 Reset": "No", "640 AO-Description": "NI DAC", "Source": "PRIME", "445 AO-MinVolts": "0.0000", "PIEZO-Name": "Stage", "445 AO-Description": "NI DAC", "PRIME-CircularBufferFrameCount": "46", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "520 AO-Volts": "0.0000", "528 Shutter-TriggerSampleRate": "100000", "XPositionUm": 19731.9, "OpticalPath-State": "4", "PRIME-PP 3 THRESHOLD": "80", "PRIME-PVCAM Version": "3.7.1", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Gain": "1-HDR", "640 Shutter-TriggerSequenceLength": "1000", "528 AO-SupportsTriggering": "0", "445 AO-TriggerInputLine": "/Dev1/PFI0", "OpticalPath-Name": "OpticalPath", "PRIME-PP 4 ENABLED": "Yes", "PRIME-PVCAM Adapter Version": "1.3.29", "COM3-Name": "COM3", "405 AO-MaxVolts": "5.0000", "520 Shutter-Block voltage": "false", "PRIME-Timing-ClearingTimeNs": "49344000.0000", "Core-Focus": "PIEZO", "528 AO-Block voltage": "false", "TE2000-Description": "Nikon TE2000 hub", "528 AO-MinVolts": "0.0000", "COM4-Parity": "None", "Summary": { "Slices": 11, "ChContrastMin": [ 0, 0, 0, 0, 0, 0 ], "PixelAspect": 1, "SlicesFirst": true, "Interval_ms": 0, "Time": "2019-12-18 21:36:42 -0500", "Prefix": "seq_4", "InitialPositionList": [ { "Label": "Pos0", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.21], "Focus": [17741.5], "XYStage": [ 44542.200000000004, 31636.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos1", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.22], "Focus": [17741.4], "XYStage": [ 43801.100000000006, 31637.9 ] }, "GridColumnIndex": 0 }, { "Label": "Pos2", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.5], "XYStage": [ 38561.3, 32040.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos3", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.185], "Focus": [17741.3], "XYStage": [ 37698.4, 32041.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos4", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.16], "Focus": [17741.5], "XYStage": [ 32375.4, 31758.9 ] }, "GridColumnIndex": 0 }, { "Label": "Pos5", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.17000000000002], "Focus": [17741.3], "XYStage": [ 31770.7, 31758.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos6", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.4], "XYStage": [ 26536.800000000003, 31068.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos7", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.205], "Focus": [17741.4], "XYStage": [ 25420.2, 31068.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos8", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.85], "Focus": [17741.3], "XYStage": [ 21059, 31508 ] }, "GridColumnIndex": 0 }, { "Label": "Pos9", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.82], "Focus": [17741.3], "XYStage": [ 19731.9, 31506.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos10", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.79], "Focus": [17741.4], "XYStage": [ 14528.5, 32007.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos11", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.83], "Focus": [17741.2], "XYStage": [ 13830.300000000001, 32009.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos12", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.79], "Focus": [17741.3], "XYStage": [ 8775.300000000001, 32017.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos13", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.815], "Focus": [17741.3], "XYStage": [ 8109.200000000001, 32008.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos14", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.805], "Focus": [17741.3], "XYStage": [ 3483.3, 31908.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos15", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.82], "Focus": [17741.2], "XYStage": [ 2686.8, 31903.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos16", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [211.48000000000002], "Focus": [17741.1], "XYStage": [ -1978.9, 31973.600000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos17", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [211.51500000000001], "Focus": [17741.1], "XYStage": [ -2957.8, 31982.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos18", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.195], "Focus": [17741.5], "XYStage": [ 47747.4, -576.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos19", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.23000000000002], "Focus": [17741.6], "XYStage": [ 47053.9, -576.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos20", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.205], "Focus": [17741.4], "XYStage": [ 41871.200000000004, -479.8 ] }, "GridColumnIndex": 0 }, { "Label": "Pos21", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.4], "XYStage": [ 40882.600000000006, -392 ] }, "GridColumnIndex": 0 }, { "Label": "Pos22", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.24], "Focus": [17741.4], "XYStage": [ 35905.6, -135.3 ] }, "GridColumnIndex": 0 }, { "Label": "Pos23", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.195], "Focus": [17741.5], "XYStage": [ 34990.9, -132.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos24", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.46], "Focus": [17741.4], "XYStage": [ 29678.100000000002, -958.1 ] }, "GridColumnIndex": 0 }, { "Label": "Pos25", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.44], "Focus": [17741.5], "XYStage": [ 28983.600000000002, -939.4000000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos26", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.475], "Focus": [17741.5], "XYStage": [ 23019.7, -122 ] }, "GridColumnIndex": 0 }, { "Label": "Pos27", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.44], "Focus": [17741.7], "XYStage": [ 22428, -116.80000000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos28", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.46], "Focus": [17741.7], "XYStage": [ 16364.900000000001, -181.10000000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos29", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.48000000000002], "Focus": [17741.5], "XYStage": [ 15712.1, -180 ] }, "GridColumnIndex": 0 }, { "Label": "Pos30", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.45000000000002], "Focus": [17741.7], "XYStage": [ 10346.1, 77.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos31", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.49], "Focus": [17741.4], "XYStage": [ 9755.5, 85.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos32", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.475], "Focus": [17741.5], "XYStage": [ 4775.5, 293.90000000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos33", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.445], "Focus": [17741.4], "XYStage": [ 3954.9, 293.90000000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos34", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.455], "Focus": [17741.4], "XYStage": [ -1324.9, 545.6 ] }, "GridColumnIndex": 0 }, { "Label": "Pos35", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.445], "Focus": [17741.3], "XYStage": [ -2097, 576.6 ] }, "GridColumnIndex": 0 } ], "z-step_um": 10, "GridRow": 0, "BitDepth": 16, "Depth": 2, "Channels": 6, "Source": "Micro-Manager", "ChColors": [ -16711681, -256, -65281, -1, -1, -16776961 ], "MetadataVersion": 10, "KeepShutterOpenChannels": true, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536, 65536 ], "CustomIntervals_ms": [], "Height": 1200, "MicroManagerVersion": "1.4.23 20180219", "GridColumn": 0, "UUID": "14a13bf7-c944-4672-b873-fcfbbb7ea13d", "PixelType": "GRAY16", "Width": 1200, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\Yan\\20191218-YC113-S7S8-miseq", "Frames": 1, "Comment": "", "UserName": "Nikon", "PixelSize_um": 1.1, "ROI": [ 0, 0, 1200, 1200 ], "Date": "2019-12-18", "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC", "P-DAPI-high" ], "KeepShutterOpenSlices": true, "IJType": 1, "ComputerName": "ZadorConfocal2", "Positions": 36, "PositionIndex": 9, "CameraTimeout": 20000, "TimeFirst": false }, "640 AO-Name": "AnalogIO", "Vincent-D3-Command": "Close" }, "FrameKey-0-2-7": { "COM4-StopBits": "1", "528 AO-MaxVolts": "5.0000", "XLIGHT Excitation Wheel-State": "1", "470 Shutter-Name": "AnalogIO", "Core-Shutter": "640", "555 AO-Demo": "No", "XLIGHT Dichroic-State": "2", "XYStage-StepSize-Y": "0.1000", "ZPositionUm": 268.82, "XYStage-StepSize-X": "0.1000", "PRIME-FirmwareVersion": "20.28", "PRIME-ClearCycles": "2", "520-Name": "DA Shutter", "405 Shutter-Name": "AnalogIO", "PIEZO-StepSize": "0.0050", "PRIME-ClearMode": "Pre-Exposure", "470 AO-MinVolts": "0.0000", "405 AO-SupportsTriggering": "1", "CameraChannelIndex": 0, "XYStage-StartSpeed": "500.0000", "COM9-StopBits": "2", "405 AO-Description": "NI DAC", "COM4-DelayBetweenCharsMs": "0.0000", "445 AO-Block voltage": "false", "520 AO-TriggerInputLine": "/Dev1/PFI0", "555 AO-Description": "NI DAC", "520 AO-TriggerSampleRate": "100000", "COM4-Verbose": "1", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM4-Name": "COM4", "405 Shutter-Volts": "0.0000", "Vincent-D3-Port": "COM1", "PRIME-PP 4 MIN ADU AFFECTED": "200", "470 Shutter-MinVolts": "0.0000", "PRIME-PP 3 ENABLED": "Yes", "470 AO-Description": "NI DAC", "PRIME-Description": "PVCAM API device adapter", "520 Shutter-MaxVolts": "5.0000", "520 Shutter-Volts": "0.0000", "528 AO-Sequenceable": "1", "PRIME-Binning": "1x1", "555 Shutter-MaxVolts": "5.0000", "528 Shutter-TriggerSequenceLength": "1000", "405 Shutter-Block voltage": "false", "XLIGHT Spin motor-State": "1", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "Vincent-D3-Time to open (ms)": "35.0000", "Vincent-D3-Time to close (ms)": "35.0000", "445 AO-TriggerSampleRate": "100000", "640 Shutter-IOChannel": "Dev1/ao14", "555 AO-Sequenceable": "1", "520 AO-Name": "AnalogIO", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "470 AO-Block voltage": "false", "COM1-Verbose": "1", "COM9-AnswerTimeout": "10000.0000", "528 Shutter-Name": "AnalogIO", "PRIME-Trigger Timeout (secs)": "10", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "NextFrame": 0, "470 AO-TriggerSequenceLength": "1000", "PRIME-TransposeXY": "0", "445 AO-TriggerSequenceLength": "1000", "COM1-Name": "COM1", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "COM9-DataBits": "8", "COM1-AnswerTimeout": "500.0000", "Camera": "", "TE2000-Version": "V2.50-V2.50-V2.10", "528 Shutter-Volts": "0.0000", "XLIGHT Hub-Name": "XLIGHT Hub", "PIEZO-Description": "Ludl stage driver adapter", "PositionName": "Pos9", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSampleRate": "100000", "PRIME-FanSpeedSetpoint": "High", "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "405 AO-TriggerSampleRate": "100000", "470 Shutter-Block voltage": "false", "470-State": "0", "COM3-Parity": "None", "405 AO-Volts": "0.0000", "PRIME-TransposeMirrorY": "0", "PRIME-ChipName": "GS144BSI", "470 AO-Volts": "0.0000", "PRIME-TransposeMirrorX": "0", "445 Shutter-SupportsTriggering": "0", "470 Shutter-SupportsTriggering": "0", "XLIGHT Hub-Description": "XLIGHT hub", "PRIME-PreampOffLimit": "10000", "470 AO-Sequenceable": "1", "528 Shutter-MaxVolts": "5.0000", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "Core-XYStage": "XYStage", "470 AO-TriggerSampleRate": "100000", "PRIME-Timing-PreTriggerDelayNs": "24672000.0000", "528-DA Device": "528 Shutter", "Focus-Description": "Nikon TE2000 focus adapter", "FilterBlock-State": "5", "520 AO-Demo": "No", "COM3-DelayBetweenCharsMs": "0.0000", "470 Shutter-Volts": "0.0000", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "PRIME-PP 2 THRESHOLD": "125", "PRIME-PP 4 THRESHOLD": "75", "XYStage-Acceleration": "4.0000", "470 Shutter-Demo": "No", "PRIME-Name": "PMPCIECam00", "640-DA Device": "640 Shutter", "405 AO-Block voltage": "false", "ElapsedTime-ms": 390927, "PRIME-Offset": "100", "445 AO-IOChannel": "Dev1/ao1", "640 AO-MaxVolts": "5.0000", "PositionIndex": 9, "PRIME-PP 1 ENABLED": "Yes", "COM1-BaudRate": "9600", "640-State": "1", "COM3-Verbose": "1", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "LudlController-TransmissionDelay": "0", "PRIME-FullWellCapacity": "62000", "PRIME-SMARTStreamingEnabled": "No", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "640 Shutter-Description": "NI DAC", "528-Description": "DA device that is used as a shutter", "COM4-Description": "Serial port driver (boost:asio)", "405 Shutter-Description": "NI DAC", "TE2000-Name": "TE2000", "528 Shutter-Description": "NI DAC", "COM9-Description": "Serial port driver (boost:asio)", "LudlController-Name": "LudlController", "445 Shutter-TriggerSequenceLength": "1000", "PRIME-SerialNumber": "A18B203008", "445 AO-Volts": "0.0000", "PRIME-X-dimension": "1200", "PRIME-ShutterCloseDelay": "0", "XLIGHT Disk slider-State": "2", "Position": "Default", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 AO-IOChannel": "Dev1/ao5", "520 AO-IOChannel": "Dev1/ao4", "528 AO-TriggerSequenceLength": "1000", "405 Shutter-MaxVolts": "5.0000", "470-DA Device": "470 Shutter", "PRIME-FTCapable": "No", "Core-ChannelGroup": "Channels", "555 AO-SupportsTriggering": "0", "PRIME-Y-dimension": "1200", "Frame": 0, "555 Shutter-Sequenceable": "1", "PRIME-Timing-ExposureTimeNs": "499998000.0000", "Core-ImageProcessor": "", "528-State": "0", "520 AO-Block voltage": "false", "XLIGHT Excitation Wheel-Label": "QUAD", "640 Shutter-Demo": "No", "COM3-StopBits": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "520 Shutter-IOChannel": "Dev1/ao11", "Width": 1200, "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-SupportsTriggering": "0", "640 AO-TriggerSampleRate": "100000", "520-Description": "DA device that is used as a shutter", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "405 Shutter-Sequenceable": "1", "405 Shutter-SupportsTriggering": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-TriggerSampleRate": "100000", "640 Shutter-MaxVolts": "5.0000", "555 AO-MaxVolts": "5.0000", "520 AO-TriggerSequenceLength": "1000", "COM3-BaudRate": "9600", "COM9-BaudRate": "9600", "Vincent-D3-Channel #": "Ch. #1", "PRIME-PMode": "Normal", "OpticalPath-Label": "Left(100)", "BitDepth": 16, "555 AO-TriggerSequenceLength": "1000", "445 Shutter-Description": "NI DAC", "405 AO-Sequenceable": "1", "PRIME-AcquisitionMethod": "Callbacks", "555 AO-Name": "AnalogIO", "PRIME-ShutterMode": "Pre-Exposure", "COM3-Handshaking": "Off", "445 AO-SupportsTriggering": "0", "ROI": "0-0-1200-1200", "COM9-Verbose": "1", "PRIME-Color": "OFF", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "PRIME-ShutterOpenDelay": "0", "COM1-Parity": "None", "Slice": 7, "SlicePosition": 20, "COM9-DelayBetweenCharsMs": "0.0000", "555-Name": "DA Shutter", "PIEZO-LudlSingleAxisName": "B", "XLIGHT Emission Wheel-Label": "679/29 (A)", "640 Shutter-Name": "AnalogIO", "640 AO-Sequenceable": "1", "520 Shutter-Demo": "No", "528 Shutter-Demo": "No", "470-Name": "DA Shutter", "520-DA Device": "520 Shutter", "XYStage-Description": "Ludl XY stage driver adapter", "445 Shutter-MinVolts": "0.0000", "528 AO-TriggerSampleRate": "100000", "405 AO-MinVolts": "0.0000", "405 Shutter-TriggerSequenceLength": "1000", "Height": 1200, "555 AO-Block voltage": "false", "640 Shutter-Volts": "5.0000", "PixelType": "GRAY16", "445 Shutter-Block voltage": "false", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "520 Shutter-TriggerSequenceLength": "1000", "COM9-Name": "COM9", "Core-SLM": "", "555 AO-Volts": "0.0000", "555 Shutter-Block voltage": "false", "XYStage-Speed": "1800.0000", "520 AO-MaxVolts": "5.0000", "528 Shutter-SupportsTriggering": "0", "COM1-Description": "Serial port driver (boost:asio)", "PRIME-MetadataEnabled": "No", "520 Shutter-MinVolts": "0.0000", "470 Shutter-Sequenceable": "1", "PRIME-PreampDelay": "0", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-MinVolts": "0.0000", "ChannelIndex": 2, "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Core-AutoShutter": "1", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "Exposure-ms": 320, "PRIME-PP 2 ENABLED": "Yes", "640 Shutter-TriggerSampleRate": "100000", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "470 Shutter-IOChannel": "Dev1/ao10", "405 AO-Demo": "No", "PixelSizeUm": 1.1, "470 AO-Demo": "No", "470 Shutter-MaxVolts": "5.0000", "Vincent-D3-Name": "Vincent-D3", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FileName": "img_000000000_Il-A_007.tif", "LudlController-Reset": "Operate", "PRIME-Exposure": "320.00", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "445-Name": "DA Shutter", "555 Shutter-Name": "AnalogIO", "520 Shutter-TriggerSampleRate": "100000", "445 Shutter-IOChannel": "Dev1/ao9", "PRIME-TransposeCorrection": "0", "XYStage-StepSize": "0.1000", "445-Description": "DA device that is used as a shutter", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 AO-IOChannel": "Dev1/ao7", "520 Shutter-Name": "AnalogIO", "COM3-DataBits": "8", "640 AO-Volts": "4.5000", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "555 Shutter-IOChannel": "Dev1/ao13", "WaitInterval": null, "520 Shutter-Sequenceable": "1", "YPositionUm": 31506.2, "445-State": "0", "445-DA Device": "445 Shutter", "PRIME-CircularBufferAutoSize": "ON", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "555 AO-TriggerSampleRate": "100000", "445 Shutter-Volts": "0.0000", "XLIGHT Hub-Port": "COM3", "Core-AutoFocus": "", "COM4-Handshaking": "Off", "528 Shutter-MinVolts": "0.0000", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "445 Shutter-Name": "AnalogIO", "528 Shutter-Sequenceable": "1", "XYStage-TransposeMirrorX": "0", "XYStage-TransposeMirrorY": "0", "PRIME-ReadoutRate": "100MHz 16bit", "XYStage-Name": "Stage", "640 AO-SupportsTriggering": "0", "640-Name": "DA Shutter", "640 Shutter-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "640 AO-Block voltage": "false", "445 Shutter-Sequenceable": "1", "470 AO-MaxVolts": "5.0000", "COM4-BaudRate": "9600", "470-Description": "DA device that is used as a shutter", "520 Shutter-Description": "NI DAC", "PRIME-CCDTemperature": "-14.8500", "Time": "2019-12-18 21:36:58 -0500", "640 AO-TriggerSequenceLength": "1000", "470 AO-Name": "AnalogIO", "XLIGHT Disk slider-Label": "Disk Pos 40um", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "Binning": "1x1", "TE2000-ModelType": "1", "COM3-AnswerTimeout": "500.0000", "555 Shutter-SupportsTriggering": "0", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "TE2000-Port": "COM4", "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Description": "NI DAC", "COM4-AnswerTimeout": "500.0000", "COM1-DelayBetweenCharsMs": "0.0000", "FilterBlock-Label": "Confocal", "445 AO-Name": "AnalogIO", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-Timing-ReadoutTimeNs": "24672000.0000", "528 AO-Demo": "No", "520 AO-Sequenceable": "1", "555-Description": "DA device that is used as a shutter", "405-DA Device": "405 Shutter", "405 AO-TriggerSequenceLength": "1000", "640 AO-MinVolts": "0.0000", "640 Shutter-Block voltage": "false", "FrameIndex": 0, "PRIME-Port": "CMOS", "555 AO-MinVolts": "0.0000", "COM3-Description": "Serial port driver (boost:asio)", "528 AO-Description": "NI DAC", "555 AO-IOChannel": "Dev1/ao6", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "555 AO-TriggerInputLine": "/Dev1/PFI0", "PRIME-ActualInterval-ms": "390.5200", "LudlController-Port": "COM9", "445 Shutter-Demo": "No", "PRIME-Trigger-Expose Out-Mux": "1", "SliceIndex": 7, "PRIME-CCDTemperatureSetPoint": "-15.0000", "405 Shutter-MinVolts": "0.0000", "PRIME-ExposeOutMode": "First Row", "AxisPositions": null, "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Volts": "0.0000", "528-Name": "DA Shutter", "555-State": "0", "XLIGHT Dichroic-Label": "Di03-R635-t1", "445 AO-Sequenceable": "1", "405-Name": "DA Shutter", "405 AO-IOChannel": "Dev1/ao0", "Core-Camera": "PRIME", "Focus-Name": "Focus", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "405 Shutter-IOChannel": "Dev1/ao8", "640 AO-Demo": "No", "640 Shutter-SupportsTriggering": "0", "445 AO-Demo": "No", "555-DA Device": "555 Shutter", "470 Shutter-Description": "NI DAC", "528 AO-Volts": "0.0000", "COM1-DataBits": "8", "Vincent-D3-Address": "x", "520 AO-SupportsTriggering": "0", "405-Description": "DA device that is used as a shutter", "640 Shutter-MinVolts": "0.0000", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "405 AO-Name": "AnalogIO", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "640 AO-TriggerInputLine": "/Dev1/PFI0", "405 AO-TriggerInputLine": "/Dev1/PFI0", "640-Description": "DA device that is used as a shutter", "445 Shutter-TriggerSampleRate": "100000", "470 Shutter-TriggerSequenceLength": "1000", "COM1-StopBits": "1", "520 Shutter-SupportsTriggering": "0", "405-State": "0", "Core-Initialize": "1", "XLIGHT Emission Wheel-State": "3", "520 AO-Description": "NI DAC", "528 AO-TriggerInputLine": "/Dev1/PFI0", "445 Shutter-MaxVolts": "5.0000", "COM9-Handshaking": "Off", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "XLIGHT Spin motor-Label": "On (spinning)", "PRIME-PP 5 ENABLED": "No", "UUID": "d7a02bcd-a0ca-4590-9b0e-7b8cf9c30a02", "PRIME-TriggerMode": "Internal Trigger", "COM1-Handshaking": "Off", "PRIME-Timing-PostTriggerDelayNs": "49344000.0000", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "Focus-Position": "17741.5000", "528 Shutter-Block voltage": "false", "Channel": "Il-A", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "555 Shutter-Demo": "No", "405 Shutter-Demo": "No", "528 AO-Name": "AnalogIO", "520-State": "0", "Core-Galvo": "", "405 Shutter-TriggerSampleRate": "100000", "Focus-FocusStepSize_(nm)": "100", "PRIME-PixelType": "16bit", "COM4-DataBits": "8", "470 AO-IOChannel": "Dev1/ao2", "520 AO-MinVolts": "0.0000", "COM9-Parity": "None", "PRIME-CircularBufferEnabled": "ON", "PRIME-PP 6 Reset": "No", "640 AO-Description": "NI DAC", "Source": "PRIME", "445 AO-MinVolts": "0.0000", "PIEZO-Name": "Stage", "445 AO-Description": "NI DAC", "PRIME-CircularBufferFrameCount": "46", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "520 AO-Volts": "0.0000", "528 Shutter-TriggerSampleRate": "100000", "XPositionUm": 19731.9, "OpticalPath-State": "4", "PRIME-PP 3 THRESHOLD": "80", "PRIME-PVCAM Version": "3.7.1", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Gain": "1-HDR", "640 Shutter-TriggerSequenceLength": "1000", "528 AO-SupportsTriggering": "0", "445 AO-TriggerInputLine": "/Dev1/PFI0", "OpticalPath-Name": "OpticalPath", "PRIME-PP 4 ENABLED": "Yes", "PRIME-PVCAM Adapter Version": "1.3.29", "COM3-Name": "COM3", "405 AO-MaxVolts": "5.0000", "520 Shutter-Block voltage": "false", "PRIME-Timing-ClearingTimeNs": "49344000.0000", "Core-Focus": "PIEZO", "528 AO-Block voltage": "false", "TE2000-Description": "Nikon TE2000 hub", "528 AO-MinVolts": "0.0000", "COM4-Parity": "None", "Summary": { "Slices": 11, "ChContrastMin": [ 0, 0, 0, 0, 0, 0 ], "PixelAspect": 1, "SlicesFirst": true, "Interval_ms": 0, "Time": "2019-12-18 21:36:42 -0500", "Prefix": "seq_4", "InitialPositionList": [ { "Label": "Pos0", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.21], "Focus": [17741.5], "XYStage": [ 44542.200000000004, 31636.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos1", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.22], "Focus": [17741.4], "XYStage": [ 43801.100000000006, 31637.9 ] }, "GridColumnIndex": 0 }, { "Label": "Pos2", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.5], "XYStage": [ 38561.3, 32040.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos3", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.185], "Focus": [17741.3], "XYStage": [ 37698.4, 32041.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos4", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.16], "Focus": [17741.5], "XYStage": [ 32375.4, 31758.9 ] }, "GridColumnIndex": 0 }, { "Label": "Pos5", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.17000000000002], "Focus": [17741.3], "XYStage": [ 31770.7, 31758.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos6", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.4], "XYStage": [ 26536.800000000003, 31068.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos7", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.205], "Focus": [17741.4], "XYStage": [ 25420.2, 31068.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos8", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.85], "Focus": [17741.3], "XYStage": [ 21059, 31508 ] }, "GridColumnIndex": 0 }, { "Label": "Pos9", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.82], "Focus": [17741.3], "XYStage": [ 19731.9, 31506.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos10", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.79], "Focus": [17741.4], "XYStage": [ 14528.5, 32007.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos11", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.83], "Focus": [17741.2], "XYStage": [ 13830.300000000001, 32009.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos12", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.79], "Focus": [17741.3], "XYStage": [ 8775.300000000001, 32017.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos13", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.815], "Focus": [17741.3], "XYStage": [ 8109.200000000001, 32008.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos14", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.805], "Focus": [17741.3], "XYStage": [ 3483.3, 31908.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos15", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.82], "Focus": [17741.2], "XYStage": [ 2686.8, 31903.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos16", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [211.48000000000002], "Focus": [17741.1], "XYStage": [ -1978.9, 31973.600000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos17", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [211.51500000000001], "Focus": [17741.1], "XYStage": [ -2957.8, 31982.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos18", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.195], "Focus": [17741.5], "XYStage": [ 47747.4, -576.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos19", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.23000000000002], "Focus": [17741.6], "XYStage": [ 47053.9, -576.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos20", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.205], "Focus": [17741.4], "XYStage": [ 41871.200000000004, -479.8 ] }, "GridColumnIndex": 0 }, { "Label": "Pos21", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.4], "XYStage": [ 40882.600000000006, -392 ] }, "GridColumnIndex": 0 }, { "Label": "Pos22", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.24], "Focus": [17741.4], "XYStage": [ 35905.6, -135.3 ] }, "GridColumnIndex": 0 }, { "Label": "Pos23", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.195], "Focus": [17741.5], "XYStage": [ 34990.9, -132.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos24", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.46], "Focus": [17741.4], "XYStage": [ 29678.100000000002, -958.1 ] }, "GridColumnIndex": 0 }, { "Label": "Pos25", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.44], "Focus": [17741.5], "XYStage": [ 28983.600000000002, -939.4000000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos26", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.475], "Focus": [17741.5], "XYStage": [ 23019.7, -122 ] }, "GridColumnIndex": 0 }, { "Label": "Pos27", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.44], "Focus": [17741.7], "XYStage": [ 22428, -116.80000000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos28", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.46], "Focus": [17741.7], "XYStage": [ 16364.900000000001, -181.10000000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos29", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.48000000000002], "Focus": [17741.5], "XYStage": [ 15712.1, -180 ] }, "GridColumnIndex": 0 }, { "Label": "Pos30", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.45000000000002], "Focus": [17741.7], "XYStage": [ 10346.1, 77.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos31", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.49], "Focus": [17741.4], "XYStage": [ 9755.5, 85.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos32", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.475], "Focus": [17741.5], "XYStage": [ 4775.5, 293.90000000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos33", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.445], "Focus": [17741.4], "XYStage": [ 3954.9, 293.90000000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos34", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.455], "Focus": [17741.4], "XYStage": [ -1324.9, 545.6 ] }, "GridColumnIndex": 0 }, { "Label": "Pos35", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.445], "Focus": [17741.3], "XYStage": [ -2097, 576.6 ] }, "GridColumnIndex": 0 } ], "z-step_um": 10, "GridRow": 0, "BitDepth": 16, "Depth": 2, "Channels": 6, "Source": "Micro-Manager", "ChColors": [ -16711681, -256, -65281, -1, -1, -16776961 ], "MetadataVersion": 10, "KeepShutterOpenChannels": true, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536, 65536 ], "CustomIntervals_ms": [], "Height": 1200, "MicroManagerVersion": "1.4.23 20180219", "GridColumn": 0, "UUID": "14a13bf7-c944-4672-b873-fcfbbb7ea13d", "PixelType": "GRAY16", "Width": 1200, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\Yan\\20191218-YC113-S7S8-miseq", "Frames": 1, "Comment": "", "UserName": "Nikon", "PixelSize_um": 1.1, "ROI": [ 0, 0, 1200, 1200 ], "Date": "2019-12-18", "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC", "P-DAPI-high" ], "KeepShutterOpenSlices": true, "IJType": 1, "ComputerName": "ZadorConfocal2", "Positions": 36, "PositionIndex": 9, "CameraTimeout": 20000, "TimeFirst": false }, "640 AO-Name": "AnalogIO", "Vincent-D3-Command": "Close" }, "FrameKey-0-2-8": { "COM4-StopBits": "1", "528 AO-MaxVolts": "5.0000", "XLIGHT Excitation Wheel-State": "1", "470 Shutter-Name": "AnalogIO", "Core-Shutter": "640", "555 AO-Demo": "No", "XLIGHT Dichroic-State": "2", "XYStage-StepSize-Y": "0.1000", "ZPositionUm": 278.82, "XYStage-StepSize-X": "0.1000", "PRIME-FirmwareVersion": "20.28", "PRIME-ClearCycles": "2", "520-Name": "DA Shutter", "405 Shutter-Name": "AnalogIO", "PIEZO-StepSize": "0.0050", "PRIME-ClearMode": "Pre-Exposure", "470 AO-MinVolts": "0.0000", "405 AO-SupportsTriggering": "1", "CameraChannelIndex": 0, "XYStage-StartSpeed": "500.0000", "COM9-StopBits": "2", "405 AO-Description": "NI DAC", "COM4-DelayBetweenCharsMs": "0.0000", "445 AO-Block voltage": "false", "520 AO-TriggerInputLine": "/Dev1/PFI0", "555 AO-Description": "NI DAC", "520 AO-TriggerSampleRate": "100000", "COM4-Verbose": "1", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM4-Name": "COM4", "405 Shutter-Volts": "0.0000", "Vincent-D3-Port": "COM1", "PRIME-PP 4 MIN ADU AFFECTED": "200", "470 Shutter-MinVolts": "0.0000", "PRIME-PP 3 ENABLED": "Yes", "470 AO-Description": "NI DAC", "PRIME-Description": "PVCAM API device adapter", "520 Shutter-MaxVolts": "5.0000", "520 Shutter-Volts": "0.0000", "528 AO-Sequenceable": "1", "PRIME-Binning": "1x1", "555 Shutter-MaxVolts": "5.0000", "528 Shutter-TriggerSequenceLength": "1000", "405 Shutter-Block voltage": "false", "XLIGHT Spin motor-State": "1", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "Vincent-D3-Time to open (ms)": "35.0000", "Vincent-D3-Time to close (ms)": "35.0000", "445 AO-TriggerSampleRate": "100000", "640 Shutter-IOChannel": "Dev1/ao14", "555 AO-Sequenceable": "1", "520 AO-Name": "AnalogIO", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "470 AO-Block voltage": "false", "COM1-Verbose": "1", "COM9-AnswerTimeout": "10000.0000", "528 Shutter-Name": "AnalogIO", "PRIME-Trigger Timeout (secs)": "10", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "NextFrame": 0, "470 AO-TriggerSequenceLength": "1000", "PRIME-TransposeXY": "0", "445 AO-TriggerSequenceLength": "1000", "COM1-Name": "COM1", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "COM9-DataBits": "8", "COM1-AnswerTimeout": "500.0000", "Camera": "", "TE2000-Version": "V2.50-V2.50-V2.10", "528 Shutter-Volts": "0.0000", "XLIGHT Hub-Name": "XLIGHT Hub", "PIEZO-Description": "Ludl stage driver adapter", "PositionName": "Pos9", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSampleRate": "100000", "PRIME-FanSpeedSetpoint": "High", "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "405 AO-TriggerSampleRate": "100000", "470 Shutter-Block voltage": "false", "470-State": "0", "COM3-Parity": "None", "405 AO-Volts": "0.0000", "PRIME-TransposeMirrorY": "0", "PRIME-ChipName": "GS144BSI", "470 AO-Volts": "0.0000", "PRIME-TransposeMirrorX": "0", "445 Shutter-SupportsTriggering": "0", "470 Shutter-SupportsTriggering": "0", "XLIGHT Hub-Description": "XLIGHT hub", "PRIME-PreampOffLimit": "10000", "470 AO-Sequenceable": "1", "528 Shutter-MaxVolts": "5.0000", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "Core-XYStage": "XYStage", "470 AO-TriggerSampleRate": "100000", "PRIME-Timing-PreTriggerDelayNs": "24672000.0000", "528-DA Device": "528 Shutter", "Focus-Description": "Nikon TE2000 focus adapter", "FilterBlock-State": "5", "520 AO-Demo": "No", "COM3-DelayBetweenCharsMs": "0.0000", "470 Shutter-Volts": "0.0000", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "PRIME-PP 2 THRESHOLD": "125", "PRIME-PP 4 THRESHOLD": "75", "XYStage-Acceleration": "4.0000", "470 Shutter-Demo": "No", "PRIME-Name": "PMPCIECam00", "640-DA Device": "640 Shutter", "405 AO-Block voltage": "false", "ElapsedTime-ms": 391494, "PRIME-Offset": "100", "445 AO-IOChannel": "Dev1/ao1", "640 AO-MaxVolts": "5.0000", "PositionIndex": 9, "PRIME-PP 1 ENABLED": "Yes", "COM1-BaudRate": "9600", "640-State": "1", "COM3-Verbose": "1", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "LudlController-TransmissionDelay": "0", "PRIME-FullWellCapacity": "62000", "PRIME-SMARTStreamingEnabled": "No", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "640 Shutter-Description": "NI DAC", "528-Description": "DA device that is used as a shutter", "COM4-Description": "Serial port driver (boost:asio)", "405 Shutter-Description": "NI DAC", "TE2000-Name": "TE2000", "528 Shutter-Description": "NI DAC", "COM9-Description": "Serial port driver (boost:asio)", "LudlController-Name": "LudlController", "445 Shutter-TriggerSequenceLength": "1000", "PRIME-SerialNumber": "A18B203008", "445 AO-Volts": "0.0000", "PRIME-X-dimension": "1200", "PRIME-ShutterCloseDelay": "0", "XLIGHT Disk slider-State": "2", "Position": "Default", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 AO-IOChannel": "Dev1/ao5", "520 AO-IOChannel": "Dev1/ao4", "528 AO-TriggerSequenceLength": "1000", "405 Shutter-MaxVolts": "5.0000", "470-DA Device": "470 Shutter", "PRIME-FTCapable": "No", "Core-ChannelGroup": "Channels", "555 AO-SupportsTriggering": "0", "PRIME-Y-dimension": "1200", "Frame": 0, "555 Shutter-Sequenceable": "1", "PRIME-Timing-ExposureTimeNs": "499998000.0000", "Core-ImageProcessor": "", "528-State": "0", "520 AO-Block voltage": "false", "XLIGHT Excitation Wheel-Label": "QUAD", "640 Shutter-Demo": "No", "COM3-StopBits": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "520 Shutter-IOChannel": "Dev1/ao11", "Width": 1200, "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-SupportsTriggering": "0", "640 AO-TriggerSampleRate": "100000", "520-Description": "DA device that is used as a shutter", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "405 Shutter-Sequenceable": "1", "405 Shutter-SupportsTriggering": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-TriggerSampleRate": "100000", "640 Shutter-MaxVolts": "5.0000", "555 AO-MaxVolts": "5.0000", "520 AO-TriggerSequenceLength": "1000", "COM3-BaudRate": "9600", "COM9-BaudRate": "9600", "Vincent-D3-Channel #": "Ch. #1", "PRIME-PMode": "Normal", "OpticalPath-Label": "Left(100)", "BitDepth": 16, "555 AO-TriggerSequenceLength": "1000", "445 Shutter-Description": "NI DAC", "405 AO-Sequenceable": "1", "PRIME-AcquisitionMethod": "Callbacks", "555 AO-Name": "AnalogIO", "PRIME-ShutterMode": "Pre-Exposure", "COM3-Handshaking": "Off", "445 AO-SupportsTriggering": "0", "ROI": "0-0-1200-1200", "COM9-Verbose": "1", "PRIME-Color": "OFF", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "PRIME-ShutterOpenDelay": "0", "COM1-Parity": "None", "Slice": 8, "SlicePosition": 30, "COM9-DelayBetweenCharsMs": "0.0000", "555-Name": "DA Shutter", "PIEZO-LudlSingleAxisName": "B", "XLIGHT Emission Wheel-Label": "679/29 (A)", "640 Shutter-Name": "AnalogIO", "640 AO-Sequenceable": "1", "520 Shutter-Demo": "No", "528 Shutter-Demo": "No", "470-Name": "DA Shutter", "520-DA Device": "520 Shutter", "XYStage-Description": "Ludl XY stage driver adapter", "445 Shutter-MinVolts": "0.0000", "528 AO-TriggerSampleRate": "100000", "405 AO-MinVolts": "0.0000", "405 Shutter-TriggerSequenceLength": "1000", "Height": 1200, "555 AO-Block voltage": "false", "640 Shutter-Volts": "5.0000", "PixelType": "GRAY16", "445 Shutter-Block voltage": "false", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "520 Shutter-TriggerSequenceLength": "1000", "COM9-Name": "COM9", "Core-SLM": "", "555 AO-Volts": "0.0000", "555 Shutter-Block voltage": "false", "XYStage-Speed": "1800.0000", "520 AO-MaxVolts": "5.0000", "528 Shutter-SupportsTriggering": "0", "COM1-Description": "Serial port driver (boost:asio)", "PRIME-MetadataEnabled": "No", "520 Shutter-MinVolts": "0.0000", "470 Shutter-Sequenceable": "1", "PRIME-PreampDelay": "0", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-MinVolts": "0.0000", "ChannelIndex": 2, "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Core-AutoShutter": "1", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "Exposure-ms": 320, "PRIME-PP 2 ENABLED": "Yes", "640 Shutter-TriggerSampleRate": "100000", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "470 Shutter-IOChannel": "Dev1/ao10", "405 AO-Demo": "No", "PixelSizeUm": 1.1, "470 AO-Demo": "No", "470 Shutter-MaxVolts": "5.0000", "Vincent-D3-Name": "Vincent-D3", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FileName": "img_000000000_Il-A_008.tif", "LudlController-Reset": "Operate", "PRIME-Exposure": "320.00", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "445-Name": "DA Shutter", "555 Shutter-Name": "AnalogIO", "520 Shutter-TriggerSampleRate": "100000", "445 Shutter-IOChannel": "Dev1/ao9", "PRIME-TransposeCorrection": "0", "XYStage-StepSize": "0.1000", "445-Description": "DA device that is used as a shutter", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 AO-IOChannel": "Dev1/ao7", "520 Shutter-Name": "AnalogIO", "COM3-DataBits": "8", "640 AO-Volts": "4.5000", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "555 Shutter-IOChannel": "Dev1/ao13", "WaitInterval": null, "520 Shutter-Sequenceable": "1", "YPositionUm": 31506.2, "445-State": "0", "445-DA Device": "445 Shutter", "PRIME-CircularBufferAutoSize": "ON", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "555 AO-TriggerSampleRate": "100000", "445 Shutter-Volts": "0.0000", "XLIGHT Hub-Port": "COM3", "Core-AutoFocus": "", "COM4-Handshaking": "Off", "528 Shutter-MinVolts": "0.0000", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "445 Shutter-Name": "AnalogIO", "528 Shutter-Sequenceable": "1", "XYStage-TransposeMirrorX": "0", "XYStage-TransposeMirrorY": "0", "PRIME-ReadoutRate": "100MHz 16bit", "XYStage-Name": "Stage", "640 AO-SupportsTriggering": "0", "640-Name": "DA Shutter", "640 Shutter-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "640 AO-Block voltage": "false", "445 Shutter-Sequenceable": "1", "470 AO-MaxVolts": "5.0000", "COM4-BaudRate": "9600", "470-Description": "DA device that is used as a shutter", "520 Shutter-Description": "NI DAC", "PRIME-CCDTemperature": "-14.8500", "Time": "2019-12-18 21:36:59 -0500", "640 AO-TriggerSequenceLength": "1000", "470 AO-Name": "AnalogIO", "XLIGHT Disk slider-Label": "Disk Pos 40um", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "Binning": "1x1", "TE2000-ModelType": "1", "COM3-AnswerTimeout": "500.0000", "555 Shutter-SupportsTriggering": "0", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "TE2000-Port": "COM4", "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Description": "NI DAC", "COM4-AnswerTimeout": "500.0000", "COM1-DelayBetweenCharsMs": "0.0000", "FilterBlock-Label": "Confocal", "445 AO-Name": "AnalogIO", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-Timing-ReadoutTimeNs": "24672000.0000", "528 AO-Demo": "No", "520 AO-Sequenceable": "1", "555-Description": "DA device that is used as a shutter", "405-DA Device": "405 Shutter", "405 AO-TriggerSequenceLength": "1000", "640 AO-MinVolts": "0.0000", "640 Shutter-Block voltage": "false", "FrameIndex": 0, "PRIME-Port": "CMOS", "555 AO-MinVolts": "0.0000", "COM3-Description": "Serial port driver (boost:asio)", "528 AO-Description": "NI DAC", "555 AO-IOChannel": "Dev1/ao6", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "555 AO-TriggerInputLine": "/Dev1/PFI0", "PRIME-ActualInterval-ms": "390.5200", "LudlController-Port": "COM9", "445 Shutter-Demo": "No", "PRIME-Trigger-Expose Out-Mux": "1", "SliceIndex": 8, "PRIME-CCDTemperatureSetPoint": "-15.0000", "405 Shutter-MinVolts": "0.0000", "PRIME-ExposeOutMode": "First Row", "AxisPositions": null, "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Volts": "0.0000", "528-Name": "DA Shutter", "555-State": "0", "XLIGHT Dichroic-Label": "Di03-R635-t1", "445 AO-Sequenceable": "1", "405-Name": "DA Shutter", "405 AO-IOChannel": "Dev1/ao0", "Core-Camera": "PRIME", "Focus-Name": "Focus", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "405 Shutter-IOChannel": "Dev1/ao8", "640 AO-Demo": "No", "640 Shutter-SupportsTriggering": "0", "445 AO-Demo": "No", "555-DA Device": "555 Shutter", "470 Shutter-Description": "NI DAC", "528 AO-Volts": "0.0000", "COM1-DataBits": "8", "Vincent-D3-Address": "x", "520 AO-SupportsTriggering": "0", "405-Description": "DA device that is used as a shutter", "640 Shutter-MinVolts": "0.0000", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "405 AO-Name": "AnalogIO", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "640 AO-TriggerInputLine": "/Dev1/PFI0", "405 AO-TriggerInputLine": "/Dev1/PFI0", "640-Description": "DA device that is used as a shutter", "445 Shutter-TriggerSampleRate": "100000", "470 Shutter-TriggerSequenceLength": "1000", "COM1-StopBits": "1", "520 Shutter-SupportsTriggering": "0", "405-State": "0", "Core-Initialize": "1", "XLIGHT Emission Wheel-State": "3", "520 AO-Description": "NI DAC", "528 AO-TriggerInputLine": "/Dev1/PFI0", "445 Shutter-MaxVolts": "5.0000", "COM9-Handshaking": "Off", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "XLIGHT Spin motor-Label": "On (spinning)", "PRIME-PP 5 ENABLED": "No", "UUID": "c2f476e4-bd29-48b7-a7f4-aa2b1ac35b02", "PRIME-TriggerMode": "Internal Trigger", "COM1-Handshaking": "Off", "PRIME-Timing-PostTriggerDelayNs": "49344000.0000", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "Focus-Position": "17741.5000", "528 Shutter-Block voltage": "false", "Channel": "Il-A", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "555 Shutter-Demo": "No", "405 Shutter-Demo": "No", "528 AO-Name": "AnalogIO", "520-State": "0", "Core-Galvo": "", "405 Shutter-TriggerSampleRate": "100000", "Focus-FocusStepSize_(nm)": "100", "PRIME-PixelType": "16bit", "COM4-DataBits": "8", "470 AO-IOChannel": "Dev1/ao2", "520 AO-MinVolts": "0.0000", "COM9-Parity": "None", "PRIME-CircularBufferEnabled": "ON", "PRIME-PP 6 Reset": "No", "640 AO-Description": "NI DAC", "Source": "PRIME", "445 AO-MinVolts": "0.0000", "PIEZO-Name": "Stage", "445 AO-Description": "NI DAC", "PRIME-CircularBufferFrameCount": "46", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "520 AO-Volts": "0.0000", "528 Shutter-TriggerSampleRate": "100000", "XPositionUm": 19731.9, "OpticalPath-State": "4", "PRIME-PP 3 THRESHOLD": "80", "PRIME-PVCAM Version": "3.7.1", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Gain": "1-HDR", "640 Shutter-TriggerSequenceLength": "1000", "528 AO-SupportsTriggering": "0", "445 AO-TriggerInputLine": "/Dev1/PFI0", "OpticalPath-Name": "OpticalPath", "PRIME-PP 4 ENABLED": "Yes", "PRIME-PVCAM Adapter Version": "1.3.29", "COM3-Name": "COM3", "405 AO-MaxVolts": "5.0000", "520 Shutter-Block voltage": "false", "PRIME-Timing-ClearingTimeNs": "49344000.0000", "Core-Focus": "PIEZO", "528 AO-Block voltage": "false", "TE2000-Description": "Nikon TE2000 hub", "528 AO-MinVolts": "0.0000", "COM4-Parity": "None", "Summary": { "Slices": 11, "ChContrastMin": [ 0, 0, 0, 0, 0, 0 ], "PixelAspect": 1, "SlicesFirst": true, "Interval_ms": 0, "Time": "2019-12-18 21:36:42 -0500", "Prefix": "seq_4", "InitialPositionList": [ { "Label": "Pos0", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.21], "Focus": [17741.5], "XYStage": [ 44542.200000000004, 31636.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos1", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.22], "Focus": [17741.4], "XYStage": [ 43801.100000000006, 31637.9 ] }, "GridColumnIndex": 0 }, { "Label": "Pos2", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.5], "XYStage": [ 38561.3, 32040.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos3", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.185], "Focus": [17741.3], "XYStage": [ 37698.4, 32041.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos4", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.16], "Focus": [17741.5], "XYStage": [ 32375.4, 31758.9 ] }, "GridColumnIndex": 0 }, { "Label": "Pos5", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.17000000000002], "Focus": [17741.3], "XYStage": [ 31770.7, 31758.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos6", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.4], "XYStage": [ 26536.800000000003, 31068.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos7", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.205], "Focus": [17741.4], "XYStage": [ 25420.2, 31068.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos8", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.85], "Focus": [17741.3], "XYStage": [ 21059, 31508 ] }, "GridColumnIndex": 0 }, { "Label": "Pos9", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.82], "Focus": [17741.3], "XYStage": [ 19731.9, 31506.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos10", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.79], "Focus": [17741.4], "XYStage": [ 14528.5, 32007.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos11", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.83], "Focus": [17741.2], "XYStage": [ 13830.300000000001, 32009.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos12", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.79], "Focus": [17741.3], "XYStage": [ 8775.300000000001, 32017.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos13", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.815], "Focus": [17741.3], "XYStage": [ 8109.200000000001, 32008.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos14", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.805], "Focus": [17741.3], "XYStage": [ 3483.3, 31908.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos15", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.82], "Focus": [17741.2], "XYStage": [ 2686.8, 31903.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos16", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [211.48000000000002], "Focus": [17741.1], "XYStage": [ -1978.9, 31973.600000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos17", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [211.51500000000001], "Focus": [17741.1], "XYStage": [ -2957.8, 31982.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos18", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.195], "Focus": [17741.5], "XYStage": [ 47747.4, -576.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos19", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.23000000000002], "Focus": [17741.6], "XYStage": [ 47053.9, -576.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos20", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.205], "Focus": [17741.4], "XYStage": [ 41871.200000000004, -479.8 ] }, "GridColumnIndex": 0 }, { "Label": "Pos21", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.4], "XYStage": [ 40882.600000000006, -392 ] }, "GridColumnIndex": 0 }, { "Label": "Pos22", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.24], "Focus": [17741.4], "XYStage": [ 35905.6, -135.3 ] }, "GridColumnIndex": 0 }, { "Label": "Pos23", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.195], "Focus": [17741.5], "XYStage": [ 34990.9, -132.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos24", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.46], "Focus": [17741.4], "XYStage": [ 29678.100000000002, -958.1 ] }, "GridColumnIndex": 0 }, { "Label": "Pos25", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.44], "Focus": [17741.5], "XYStage": [ 28983.600000000002, -939.4000000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos26", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.475], "Focus": [17741.5], "XYStage": [ 23019.7, -122 ] }, "GridColumnIndex": 0 }, { "Label": "Pos27", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.44], "Focus": [17741.7], "XYStage": [ 22428, -116.80000000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos28", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.46], "Focus": [17741.7], "XYStage": [ 16364.900000000001, -181.10000000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos29", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.48000000000002], "Focus": [17741.5], "XYStage": [ 15712.1, -180 ] }, "GridColumnIndex": 0 }, { "Label": "Pos30", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.45000000000002], "Focus": [17741.7], "XYStage": [ 10346.1, 77.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos31", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.49], "Focus": [17741.4], "XYStage": [ 9755.5, 85.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos32", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.475], "Focus": [17741.5], "XYStage": [ 4775.5, 293.90000000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos33", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.445], "Focus": [17741.4], "XYStage": [ 3954.9, 293.90000000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos34", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.455], "Focus": [17741.4], "XYStage": [ -1324.9, 545.6 ] }, "GridColumnIndex": 0 }, { "Label": "Pos35", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.445], "Focus": [17741.3], "XYStage": [ -2097, 576.6 ] }, "GridColumnIndex": 0 } ], "z-step_um": 10, "GridRow": 0, "BitDepth": 16, "Depth": 2, "Channels": 6, "Source": "Micro-Manager", "ChColors": [ -16711681, -256, -65281, -1, -1, -16776961 ], "MetadataVersion": 10, "KeepShutterOpenChannels": true, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536, 65536 ], "CustomIntervals_ms": [], "Height": 1200, "MicroManagerVersion": "1.4.23 20180219", "GridColumn": 0, "UUID": "14a13bf7-c944-4672-b873-fcfbbb7ea13d", "PixelType": "GRAY16", "Width": 1200, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\Yan\\20191218-YC113-S7S8-miseq", "Frames": 1, "Comment": "", "UserName": "Nikon", "PixelSize_um": 1.1, "ROI": [ 0, 0, 1200, 1200 ], "Date": "2019-12-18", "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC", "P-DAPI-high" ], "KeepShutterOpenSlices": true, "IJType": 1, "ComputerName": "ZadorConfocal2", "Positions": 36, "PositionIndex": 9, "CameraTimeout": 20000, "TimeFirst": false }, "640 AO-Name": "AnalogIO", "Vincent-D3-Command": "Close" }, "FrameKey-0-2-9": { "COM4-StopBits": "1", "528 AO-MaxVolts": "5.0000", "XLIGHT Excitation Wheel-State": "1", "470 Shutter-Name": "AnalogIO", "Core-Shutter": "640", "555 AO-Demo": "No", "XLIGHT Dichroic-State": "2", "XYStage-StepSize-Y": "0.1000", "ZPositionUm": 288.82, "XYStage-StepSize-X": "0.1000", "PRIME-FirmwareVersion": "20.28", "PRIME-ClearCycles": "2", "520-Name": "DA Shutter", "405 Shutter-Name": "AnalogIO", "PIEZO-StepSize": "0.0050", "PRIME-ClearMode": "Pre-Exposure", "470 AO-MinVolts": "0.0000", "405 AO-SupportsTriggering": "1", "CameraChannelIndex": 0, "XYStage-StartSpeed": "500.0000", "COM9-StopBits": "2", "405 AO-Description": "NI DAC", "COM4-DelayBetweenCharsMs": "0.0000", "445 AO-Block voltage": "false", "520 AO-TriggerInputLine": "/Dev1/PFI0", "555 AO-Description": "NI DAC", "520 AO-TriggerSampleRate": "100000", "COM4-Verbose": "1", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM4-Name": "COM4", "405 Shutter-Volts": "0.0000", "Vincent-D3-Port": "COM1", "PRIME-PP 4 MIN ADU AFFECTED": "200", "470 Shutter-MinVolts": "0.0000", "PRIME-PP 3 ENABLED": "Yes", "470 AO-Description": "NI DAC", "PRIME-Description": "PVCAM API device adapter", "520 Shutter-MaxVolts": "5.0000", "520 Shutter-Volts": "0.0000", "528 AO-Sequenceable": "1", "PRIME-Binning": "1x1", "555 Shutter-MaxVolts": "5.0000", "528 Shutter-TriggerSequenceLength": "1000", "405 Shutter-Block voltage": "false", "XLIGHT Spin motor-State": "1", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "Vincent-D3-Time to open (ms)": "35.0000", "Vincent-D3-Time to close (ms)": "35.0000", "445 AO-TriggerSampleRate": "100000", "640 Shutter-IOChannel": "Dev1/ao14", "555 AO-Sequenceable": "1", "520 AO-Name": "AnalogIO", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "470 AO-Block voltage": "false", "COM1-Verbose": "1", "COM9-AnswerTimeout": "10000.0000", "528 Shutter-Name": "AnalogIO", "PRIME-Trigger Timeout (secs)": "10", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "NextFrame": 0, "470 AO-TriggerSequenceLength": "1000", "PRIME-TransposeXY": "0", "445 AO-TriggerSequenceLength": "1000", "COM1-Name": "COM1", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "COM9-DataBits": "8", "COM1-AnswerTimeout": "500.0000", "Camera": "", "TE2000-Version": "V2.50-V2.50-V2.10", "528 Shutter-Volts": "0.0000", "XLIGHT Hub-Name": "XLIGHT Hub", "PIEZO-Description": "Ludl stage driver adapter", "PositionName": "Pos9", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSampleRate": "100000", "PRIME-FanSpeedSetpoint": "High", "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "405 AO-TriggerSampleRate": "100000", "470 Shutter-Block voltage": "false", "470-State": "0", "COM3-Parity": "None", "405 AO-Volts": "0.0000", "PRIME-TransposeMirrorY": "0", "PRIME-ChipName": "GS144BSI", "470 AO-Volts": "0.0000", "PRIME-TransposeMirrorX": "0", "445 Shutter-SupportsTriggering": "0", "470 Shutter-SupportsTriggering": "0", "XLIGHT Hub-Description": "XLIGHT hub", "PRIME-PreampOffLimit": "10000", "470 AO-Sequenceable": "1", "528 Shutter-MaxVolts": "5.0000", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "Core-XYStage": "XYStage", "470 AO-TriggerSampleRate": "100000", "PRIME-Timing-PreTriggerDelayNs": "24672000.0000", "528-DA Device": "528 Shutter", "Focus-Description": "Nikon TE2000 focus adapter", "FilterBlock-State": "5", "520 AO-Demo": "No", "COM3-DelayBetweenCharsMs": "0.0000", "470 Shutter-Volts": "0.0000", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "PRIME-PP 2 THRESHOLD": "125", "PRIME-PP 4 THRESHOLD": "75", "XYStage-Acceleration": "4.0000", "470 Shutter-Demo": "No", "PRIME-Name": "PMPCIECam00", "640-DA Device": "640 Shutter", "405 AO-Block voltage": "false", "ElapsedTime-ms": 392046, "PRIME-Offset": "100", "445 AO-IOChannel": "Dev1/ao1", "640 AO-MaxVolts": "5.0000", "PositionIndex": 9, "PRIME-PP 1 ENABLED": "Yes", "COM1-BaudRate": "9600", "640-State": "1", "COM3-Verbose": "1", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "LudlController-TransmissionDelay": "0", "PRIME-FullWellCapacity": "62000", "PRIME-SMARTStreamingEnabled": "No", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "640 Shutter-Description": "NI DAC", "528-Description": "DA device that is used as a shutter", "COM4-Description": "Serial port driver (boost:asio)", "405 Shutter-Description": "NI DAC", "TE2000-Name": "TE2000", "528 Shutter-Description": "NI DAC", "COM9-Description": "Serial port driver (boost:asio)", "LudlController-Name": "LudlController", "445 Shutter-TriggerSequenceLength": "1000", "PRIME-SerialNumber": "A18B203008", "445 AO-Volts": "0.0000", "PRIME-X-dimension": "1200", "PRIME-ShutterCloseDelay": "0", "XLIGHT Disk slider-State": "2", "Position": "Default", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 AO-IOChannel": "Dev1/ao5", "520 AO-IOChannel": "Dev1/ao4", "528 AO-TriggerSequenceLength": "1000", "405 Shutter-MaxVolts": "5.0000", "470-DA Device": "470 Shutter", "PRIME-FTCapable": "No", "Core-ChannelGroup": "Channels", "555 AO-SupportsTriggering": "0", "PRIME-Y-dimension": "1200", "Frame": 0, "555 Shutter-Sequenceable": "1", "PRIME-Timing-ExposureTimeNs": "499998000.0000", "Core-ImageProcessor": "", "528-State": "0", "520 AO-Block voltage": "false", "XLIGHT Excitation Wheel-Label": "QUAD", "640 Shutter-Demo": "No", "COM3-StopBits": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "520 Shutter-IOChannel": "Dev1/ao11", "Width": 1200, "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-SupportsTriggering": "0", "640 AO-TriggerSampleRate": "100000", "520-Description": "DA device that is used as a shutter", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "405 Shutter-Sequenceable": "1", "405 Shutter-SupportsTriggering": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-TriggerSampleRate": "100000", "640 Shutter-MaxVolts": "5.0000", "555 AO-MaxVolts": "5.0000", "520 AO-TriggerSequenceLength": "1000", "COM3-BaudRate": "9600", "COM9-BaudRate": "9600", "Vincent-D3-Channel #": "Ch. #1", "PRIME-PMode": "Normal", "OpticalPath-Label": "Left(100)", "BitDepth": 16, "555 AO-TriggerSequenceLength": "1000", "445 Shutter-Description": "NI DAC", "405 AO-Sequenceable": "1", "PRIME-AcquisitionMethod": "Callbacks", "555 AO-Name": "AnalogIO", "PRIME-ShutterMode": "Pre-Exposure", "COM3-Handshaking": "Off", "445 AO-SupportsTriggering": "0", "ROI": "0-0-1200-1200", "COM9-Verbose": "1", "PRIME-Color": "OFF", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "PRIME-ShutterOpenDelay": "0", "COM1-Parity": "None", "Slice": 9, "SlicePosition": 40, "COM9-DelayBetweenCharsMs": "0.0000", "555-Name": "DA Shutter", "PIEZO-LudlSingleAxisName": "B", "XLIGHT Emission Wheel-Label": "679/29 (A)", "640 Shutter-Name": "AnalogIO", "640 AO-Sequenceable": "1", "520 Shutter-Demo": "No", "528 Shutter-Demo": "No", "470-Name": "DA Shutter", "520-DA Device": "520 Shutter", "XYStage-Description": "Ludl XY stage driver adapter", "445 Shutter-MinVolts": "0.0000", "528 AO-TriggerSampleRate": "100000", "405 AO-MinVolts": "0.0000", "405 Shutter-TriggerSequenceLength": "1000", "Height": 1200, "555 AO-Block voltage": "false", "640 Shutter-Volts": "5.0000", "PixelType": "GRAY16", "445 Shutter-Block voltage": "false", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "520 Shutter-TriggerSequenceLength": "1000", "COM9-Name": "COM9", "Core-SLM": "", "555 AO-Volts": "0.0000", "555 Shutter-Block voltage": "false", "XYStage-Speed": "1800.0000", "520 AO-MaxVolts": "5.0000", "528 Shutter-SupportsTriggering": "0", "COM1-Description": "Serial port driver (boost:asio)", "PRIME-MetadataEnabled": "No", "520 Shutter-MinVolts": "0.0000", "470 Shutter-Sequenceable": "1", "PRIME-PreampDelay": "0", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-MinVolts": "0.0000", "ChannelIndex": 2, "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Core-AutoShutter": "1", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "Exposure-ms": 320, "PRIME-PP 2 ENABLED": "Yes", "640 Shutter-TriggerSampleRate": "100000", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "470 Shutter-IOChannel": "Dev1/ao10", "405 AO-Demo": "No", "PixelSizeUm": 1.1, "470 AO-Demo": "No", "470 Shutter-MaxVolts": "5.0000", "Vincent-D3-Name": "Vincent-D3", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FileName": "img_000000000_Il-A_009.tif", "LudlController-Reset": "Operate", "PRIME-Exposure": "320.00", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "445-Name": "DA Shutter", "555 Shutter-Name": "AnalogIO", "520 Shutter-TriggerSampleRate": "100000", "445 Shutter-IOChannel": "Dev1/ao9", "PRIME-TransposeCorrection": "0", "XYStage-StepSize": "0.1000", "445-Description": "DA device that is used as a shutter", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 AO-IOChannel": "Dev1/ao7", "520 Shutter-Name": "AnalogIO", "COM3-DataBits": "8", "640 AO-Volts": "4.5000", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "555 Shutter-IOChannel": "Dev1/ao13", "WaitInterval": null, "520 Shutter-Sequenceable": "1", "YPositionUm": 31506.2, "445-State": "0", "445-DA Device": "445 Shutter", "PRIME-CircularBufferAutoSize": "ON", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "555 AO-TriggerSampleRate": "100000", "445 Shutter-Volts": "0.0000", "XLIGHT Hub-Port": "COM3", "Core-AutoFocus": "", "COM4-Handshaking": "Off", "528 Shutter-MinVolts": "0.0000", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "445 Shutter-Name": "AnalogIO", "528 Shutter-Sequenceable": "1", "XYStage-TransposeMirrorX": "0", "XYStage-TransposeMirrorY": "0", "PRIME-ReadoutRate": "100MHz 16bit", "XYStage-Name": "Stage", "640 AO-SupportsTriggering": "0", "640-Name": "DA Shutter", "640 Shutter-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "640 AO-Block voltage": "false", "445 Shutter-Sequenceable": "1", "470 AO-MaxVolts": "5.0000", "COM4-BaudRate": "9600", "470-Description": "DA device that is used as a shutter", "520 Shutter-Description": "NI DAC", "PRIME-CCDTemperature": "-14.8500", "Time": "2019-12-18 21:36:59 -0500", "640 AO-TriggerSequenceLength": "1000", "470 AO-Name": "AnalogIO", "XLIGHT Disk slider-Label": "Disk Pos 40um", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "Binning": "1x1", "TE2000-ModelType": "1", "COM3-AnswerTimeout": "500.0000", "555 Shutter-SupportsTriggering": "0", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "TE2000-Port": "COM4", "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Description": "NI DAC", "COM4-AnswerTimeout": "500.0000", "COM1-DelayBetweenCharsMs": "0.0000", "FilterBlock-Label": "Confocal", "445 AO-Name": "AnalogIO", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-Timing-ReadoutTimeNs": "24672000.0000", "528 AO-Demo": "No", "520 AO-Sequenceable": "1", "555-Description": "DA device that is used as a shutter", "405-DA Device": "405 Shutter", "405 AO-TriggerSequenceLength": "1000", "640 AO-MinVolts": "0.0000", "640 Shutter-Block voltage": "false", "FrameIndex": 0, "PRIME-Port": "CMOS", "555 AO-MinVolts": "0.0000", "COM3-Description": "Serial port driver (boost:asio)", "528 AO-Description": "NI DAC", "555 AO-IOChannel": "Dev1/ao6", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "555 AO-TriggerInputLine": "/Dev1/PFI0", "PRIME-ActualInterval-ms": "390.5200", "LudlController-Port": "COM9", "445 Shutter-Demo": "No", "PRIME-Trigger-Expose Out-Mux": "1", "SliceIndex": 9, "PRIME-CCDTemperatureSetPoint": "-15.0000", "405 Shutter-MinVolts": "0.0000", "PRIME-ExposeOutMode": "First Row", "AxisPositions": null, "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Volts": "0.0000", "528-Name": "DA Shutter", "555-State": "0", "XLIGHT Dichroic-Label": "Di03-R635-t1", "445 AO-Sequenceable": "1", "405-Name": "DA Shutter", "405 AO-IOChannel": "Dev1/ao0", "Core-Camera": "PRIME", "Focus-Name": "Focus", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "405 Shutter-IOChannel": "Dev1/ao8", "640 AO-Demo": "No", "640 Shutter-SupportsTriggering": "0", "445 AO-Demo": "No", "555-DA Device": "555 Shutter", "470 Shutter-Description": "NI DAC", "528 AO-Volts": "0.0000", "COM1-DataBits": "8", "Vincent-D3-Address": "x", "520 AO-SupportsTriggering": "0", "405-Description": "DA device that is used as a shutter", "640 Shutter-MinVolts": "0.0000", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "405 AO-Name": "AnalogIO", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "640 AO-TriggerInputLine": "/Dev1/PFI0", "405 AO-TriggerInputLine": "/Dev1/PFI0", "640-Description": "DA device that is used as a shutter", "445 Shutter-TriggerSampleRate": "100000", "470 Shutter-TriggerSequenceLength": "1000", "COM1-StopBits": "1", "520 Shutter-SupportsTriggering": "0", "405-State": "0", "Core-Initialize": "1", "XLIGHT Emission Wheel-State": "3", "520 AO-Description": "NI DAC", "528 AO-TriggerInputLine": "/Dev1/PFI0", "445 Shutter-MaxVolts": "5.0000", "COM9-Handshaking": "Off", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "XLIGHT Spin motor-Label": "On (spinning)", "PRIME-PP 5 ENABLED": "No", "UUID": "4b0cd9f0-f475-43bc-816f-cba9abc0e809", "PRIME-TriggerMode": "Internal Trigger", "COM1-Handshaking": "Off", "PRIME-Timing-PostTriggerDelayNs": "49344000.0000", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "Focus-Position": "17741.5000", "528 Shutter-Block voltage": "false", "Channel": "Il-A", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "555 Shutter-Demo": "No", "405 Shutter-Demo": "No", "528 AO-Name": "AnalogIO", "520-State": "0", "Core-Galvo": "", "405 Shutter-TriggerSampleRate": "100000", "Focus-FocusStepSize_(nm)": "100", "PRIME-PixelType": "16bit", "COM4-DataBits": "8", "470 AO-IOChannel": "Dev1/ao2", "520 AO-MinVolts": "0.0000", "COM9-Parity": "None", "PRIME-CircularBufferEnabled": "ON", "PRIME-PP 6 Reset": "No", "640 AO-Description": "NI DAC", "Source": "PRIME", "445 AO-MinVolts": "0.0000", "PIEZO-Name": "Stage", "445 AO-Description": "NI DAC", "PRIME-CircularBufferFrameCount": "46", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "520 AO-Volts": "0.0000", "528 Shutter-TriggerSampleRate": "100000", "XPositionUm": 19731.9, "OpticalPath-State": "4", "PRIME-PP 3 THRESHOLD": "80", "PRIME-PVCAM Version": "3.7.1", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Gain": "1-HDR", "640 Shutter-TriggerSequenceLength": "1000", "528 AO-SupportsTriggering": "0", "445 AO-TriggerInputLine": "/Dev1/PFI0", "OpticalPath-Name": "OpticalPath", "PRIME-PP 4 ENABLED": "Yes", "PRIME-PVCAM Adapter Version": "1.3.29", "COM3-Name": "COM3", "405 AO-MaxVolts": "5.0000", "520 Shutter-Block voltage": "false", "PRIME-Timing-ClearingTimeNs": "49344000.0000", "Core-Focus": "PIEZO", "528 AO-Block voltage": "false", "TE2000-Description": "Nikon TE2000 hub", "528 AO-MinVolts": "0.0000", "COM4-Parity": "None", "Summary": { "Slices": 11, "ChContrastMin": [ 0, 0, 0, 0, 0, 0 ], "PixelAspect": 1, "SlicesFirst": true, "Interval_ms": 0, "Time": "2019-12-18 21:36:42 -0500", "Prefix": "seq_4", "InitialPositionList": [ { "Label": "Pos0", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.21], "Focus": [17741.5], "XYStage": [ 44542.200000000004, 31636.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos1", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.22], "Focus": [17741.4], "XYStage": [ 43801.100000000006, 31637.9 ] }, "GridColumnIndex": 0 }, { "Label": "Pos2", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.5], "XYStage": [ 38561.3, 32040.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos3", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.185], "Focus": [17741.3], "XYStage": [ 37698.4, 32041.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos4", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.16], "Focus": [17741.5], "XYStage": [ 32375.4, 31758.9 ] }, "GridColumnIndex": 0 }, { "Label": "Pos5", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.17000000000002], "Focus": [17741.3], "XYStage": [ 31770.7, 31758.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos6", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.4], "XYStage": [ 26536.800000000003, 31068.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos7", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.205], "Focus": [17741.4], "XYStage": [ 25420.2, 31068.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos8", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.85], "Focus": [17741.3], "XYStage": [ 21059, 31508 ] }, "GridColumnIndex": 0 }, { "Label": "Pos9", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.82], "Focus": [17741.3], "XYStage": [ 19731.9, 31506.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos10", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.79], "Focus": [17741.4], "XYStage": [ 14528.5, 32007.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos11", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.83], "Focus": [17741.2], "XYStage": [ 13830.300000000001, 32009.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos12", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.79], "Focus": [17741.3], "XYStage": [ 8775.300000000001, 32017.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos13", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.815], "Focus": [17741.3], "XYStage": [ 8109.200000000001, 32008.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos14", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.805], "Focus": [17741.3], "XYStage": [ 3483.3, 31908.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos15", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.82], "Focus": [17741.2], "XYStage": [ 2686.8, 31903.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos16", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [211.48000000000002], "Focus": [17741.1], "XYStage": [ -1978.9, 31973.600000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos17", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [211.51500000000001], "Focus": [17741.1], "XYStage": [ -2957.8, 31982.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos18", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.195], "Focus": [17741.5], "XYStage": [ 47747.4, -576.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos19", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.23000000000002], "Focus": [17741.6], "XYStage": [ 47053.9, -576.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos20", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.205], "Focus": [17741.4], "XYStage": [ 41871.200000000004, -479.8 ] }, "GridColumnIndex": 0 }, { "Label": "Pos21", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.4], "XYStage": [ 40882.600000000006, -392 ] }, "GridColumnIndex": 0 }, { "Label": "Pos22", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.24], "Focus": [17741.4], "XYStage": [ 35905.6, -135.3 ] }, "GridColumnIndex": 0 }, { "Label": "Pos23", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.195], "Focus": [17741.5], "XYStage": [ 34990.9, -132.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos24", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.46], "Focus": [17741.4], "XYStage": [ 29678.100000000002, -958.1 ] }, "GridColumnIndex": 0 }, { "Label": "Pos25", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.44], "Focus": [17741.5], "XYStage": [ 28983.600000000002, -939.4000000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos26", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.475], "Focus": [17741.5], "XYStage": [ 23019.7, -122 ] }, "GridColumnIndex": 0 }, { "Label": "Pos27", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.44], "Focus": [17741.7], "XYStage": [ 22428, -116.80000000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos28", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.46], "Focus": [17741.7], "XYStage": [ 16364.900000000001, -181.10000000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos29", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.48000000000002], "Focus": [17741.5], "XYStage": [ 15712.1, -180 ] }, "GridColumnIndex": 0 }, { "Label": "Pos30", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.45000000000002], "Focus": [17741.7], "XYStage": [ 10346.1, 77.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos31", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.49], "Focus": [17741.4], "XYStage": [ 9755.5, 85.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos32", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.475], "Focus": [17741.5], "XYStage": [ 4775.5, 293.90000000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos33", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.445], "Focus": [17741.4], "XYStage": [ 3954.9, 293.90000000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos34", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.455], "Focus": [17741.4], "XYStage": [ -1324.9, 545.6 ] }, "GridColumnIndex": 0 }, { "Label": "Pos35", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.445], "Focus": [17741.3], "XYStage": [ -2097, 576.6 ] }, "GridColumnIndex": 0 } ], "z-step_um": 10, "GridRow": 0, "BitDepth": 16, "Depth": 2, "Channels": 6, "Source": "Micro-Manager", "ChColors": [ -16711681, -256, -65281, -1, -1, -16776961 ], "MetadataVersion": 10, "KeepShutterOpenChannels": true, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536, 65536 ], "CustomIntervals_ms": [], "Height": 1200, "MicroManagerVersion": "1.4.23 20180219", "GridColumn": 0, "UUID": "14a13bf7-c944-4672-b873-fcfbbb7ea13d", "PixelType": "GRAY16", "Width": 1200, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\Yan\\20191218-YC113-S7S8-miseq", "Frames": 1, "Comment": "", "UserName": "Nikon", "PixelSize_um": 1.1, "ROI": [ 0, 0, 1200, 1200 ], "Date": "2019-12-18", "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC", "P-DAPI-high" ], "KeepShutterOpenSlices": true, "IJType": 1, "ComputerName": "ZadorConfocal2", "Positions": 36, "PositionIndex": 9, "CameraTimeout": 20000, "TimeFirst": false }, "640 AO-Name": "AnalogIO", "Vincent-D3-Command": "Close" }, "FrameKey-0-2-10": { "COM4-StopBits": "1", "528 AO-MaxVolts": "5.0000", "XLIGHT Excitation Wheel-State": "1", "470 Shutter-Name": "AnalogIO", "Core-Shutter": "640", "555 AO-Demo": "No", "XLIGHT Dichroic-State": "2", "XYStage-StepSize-Y": "0.1000", "ZPositionUm": 298.82, "XYStage-StepSize-X": "0.1000", "PRIME-FirmwareVersion": "20.28", "PRIME-ClearCycles": "2", "520-Name": "DA Shutter", "405 Shutter-Name": "AnalogIO", "PIEZO-StepSize": "0.0050", "PRIME-ClearMode": "Pre-Exposure", "470 AO-MinVolts": "0.0000", "405 AO-SupportsTriggering": "1", "CameraChannelIndex": 0, "XYStage-StartSpeed": "500.0000", "COM9-StopBits": "2", "405 AO-Description": "NI DAC", "COM4-DelayBetweenCharsMs": "0.0000", "445 AO-Block voltage": "false", "520 AO-TriggerInputLine": "/Dev1/PFI0", "555 AO-Description": "NI DAC", "520 AO-TriggerSampleRate": "100000", "COM4-Verbose": "1", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM4-Name": "COM4", "405 Shutter-Volts": "0.0000", "Vincent-D3-Port": "COM1", "PRIME-PP 4 MIN ADU AFFECTED": "200", "470 Shutter-MinVolts": "0.0000", "PRIME-PP 3 ENABLED": "Yes", "470 AO-Description": "NI DAC", "PRIME-Description": "PVCAM API device adapter", "520 Shutter-MaxVolts": "5.0000", "520 Shutter-Volts": "0.0000", "528 AO-Sequenceable": "1", "PRIME-Binning": "1x1", "555 Shutter-MaxVolts": "5.0000", "528 Shutter-TriggerSequenceLength": "1000", "405 Shutter-Block voltage": "false", "XLIGHT Spin motor-State": "1", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "Vincent-D3-Time to open (ms)": "35.0000", "Vincent-D3-Time to close (ms)": "35.0000", "445 AO-TriggerSampleRate": "100000", "640 Shutter-IOChannel": "Dev1/ao14", "555 AO-Sequenceable": "1", "520 AO-Name": "AnalogIO", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "470 AO-Block voltage": "false", "COM1-Verbose": "1", "COM9-AnswerTimeout": "10000.0000", "528 Shutter-Name": "AnalogIO", "PRIME-Trigger Timeout (secs)": "10", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "NextFrame": 0, "470 AO-TriggerSequenceLength": "1000", "PRIME-TransposeXY": "0", "445 AO-TriggerSequenceLength": "1000", "COM1-Name": "COM1", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "COM9-DataBits": "8", "COM1-AnswerTimeout": "500.0000", "Camera": "", "TE2000-Version": "V2.50-V2.50-V2.10", "528 Shutter-Volts": "0.0000", "XLIGHT Hub-Name": "XLIGHT Hub", "PIEZO-Description": "Ludl stage driver adapter", "PositionName": "Pos9", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSampleRate": "100000", "PRIME-FanSpeedSetpoint": "High", "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "405 AO-TriggerSampleRate": "100000", "470 Shutter-Block voltage": "false", "470-State": "0", "COM3-Parity": "None", "405 AO-Volts": "0.0000", "PRIME-TransposeMirrorY": "0", "PRIME-ChipName": "GS144BSI", "470 AO-Volts": "0.0000", "PRIME-TransposeMirrorX": "0", "445 Shutter-SupportsTriggering": "0", "470 Shutter-SupportsTriggering": "0", "XLIGHT Hub-Description": "XLIGHT hub", "PRIME-PreampOffLimit": "10000", "470 AO-Sequenceable": "1", "528 Shutter-MaxVolts": "5.0000", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "Core-XYStage": "XYStage", "470 AO-TriggerSampleRate": "100000", "PRIME-Timing-PreTriggerDelayNs": "24672000.0000", "528-DA Device": "528 Shutter", "Focus-Description": "Nikon TE2000 focus adapter", "FilterBlock-State": "5", "520 AO-Demo": "No", "COM3-DelayBetweenCharsMs": "0.0000", "470 Shutter-Volts": "0.0000", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "PRIME-PP 2 THRESHOLD": "125", "PRIME-PP 4 THRESHOLD": "75", "XYStage-Acceleration": "4.0000", "470 Shutter-Demo": "No", "PRIME-Name": "PMPCIECam00", "640-DA Device": "640 Shutter", "405 AO-Block voltage": "false", "ElapsedTime-ms": 392658, "PRIME-Offset": "100", "445 AO-IOChannel": "Dev1/ao1", "640 AO-MaxVolts": "5.0000", "PositionIndex": 9, "PRIME-PP 1 ENABLED": "Yes", "COM1-BaudRate": "9600", "640-State": "1", "COM3-Verbose": "1", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "LudlController-TransmissionDelay": "0", "PRIME-FullWellCapacity": "62000", "PRIME-SMARTStreamingEnabled": "No", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "640 Shutter-Description": "NI DAC", "528-Description": "DA device that is used as a shutter", "COM4-Description": "Serial port driver (boost:asio)", "405 Shutter-Description": "NI DAC", "TE2000-Name": "TE2000", "528 Shutter-Description": "NI DAC", "COM9-Description": "Serial port driver (boost:asio)", "LudlController-Name": "LudlController", "445 Shutter-TriggerSequenceLength": "1000", "PRIME-SerialNumber": "A18B203008", "445 AO-Volts": "0.0000", "PRIME-X-dimension": "1200", "PRIME-ShutterCloseDelay": "0", "XLIGHT Disk slider-State": "2", "Position": "Default", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 AO-IOChannel": "Dev1/ao5", "520 AO-IOChannel": "Dev1/ao4", "528 AO-TriggerSequenceLength": "1000", "405 Shutter-MaxVolts": "5.0000", "470-DA Device": "470 Shutter", "PRIME-FTCapable": "No", "Core-ChannelGroup": "Channels", "555 AO-SupportsTriggering": "0", "PRIME-Y-dimension": "1200", "Frame": 0, "555 Shutter-Sequenceable": "1", "PRIME-Timing-ExposureTimeNs": "499998000.0000", "Core-ImageProcessor": "", "528-State": "0", "520 AO-Block voltage": "false", "XLIGHT Excitation Wheel-Label": "QUAD", "640 Shutter-Demo": "No", "COM3-StopBits": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "520 Shutter-IOChannel": "Dev1/ao11", "Width": 1200, "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-SupportsTriggering": "0", "640 AO-TriggerSampleRate": "100000", "520-Description": "DA device that is used as a shutter", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "405 Shutter-Sequenceable": "1", "405 Shutter-SupportsTriggering": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-TriggerSampleRate": "100000", "640 Shutter-MaxVolts": "5.0000", "555 AO-MaxVolts": "5.0000", "520 AO-TriggerSequenceLength": "1000", "COM3-BaudRate": "9600", "COM9-BaudRate": "9600", "Vincent-D3-Channel #": "Ch. #1", "PRIME-PMode": "Normal", "OpticalPath-Label": "Left(100)", "BitDepth": 16, "555 AO-TriggerSequenceLength": "1000", "445 Shutter-Description": "NI DAC", "405 AO-Sequenceable": "1", "PRIME-AcquisitionMethod": "Callbacks", "555 AO-Name": "AnalogIO", "PRIME-ShutterMode": "Pre-Exposure", "COM3-Handshaking": "Off", "445 AO-SupportsTriggering": "0", "ROI": "0-0-1200-1200", "COM9-Verbose": "1", "PRIME-Color": "OFF", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "PRIME-ShutterOpenDelay": "0", "COM1-Parity": "None", "Slice": 10, "SlicePosition": 50, "COM9-DelayBetweenCharsMs": "0.0000", "555-Name": "DA Shutter", "PIEZO-LudlSingleAxisName": "B", "XLIGHT Emission Wheel-Label": "679/29 (A)", "640 Shutter-Name": "AnalogIO", "640 AO-Sequenceable": "1", "520 Shutter-Demo": "No", "528 Shutter-Demo": "No", "470-Name": "DA Shutter", "520-DA Device": "520 Shutter", "XYStage-Description": "Ludl XY stage driver adapter", "445 Shutter-MinVolts": "0.0000", "528 AO-TriggerSampleRate": "100000", "405 AO-MinVolts": "0.0000", "405 Shutter-TriggerSequenceLength": "1000", "Height": 1200, "555 AO-Block voltage": "false", "640 Shutter-Volts": "5.0000", "PixelType": "GRAY16", "445 Shutter-Block voltage": "false", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "520 Shutter-TriggerSequenceLength": "1000", "COM9-Name": "COM9", "Core-SLM": "", "555 AO-Volts": "0.0000", "555 Shutter-Block voltage": "false", "XYStage-Speed": "1800.0000", "520 AO-MaxVolts": "5.0000", "528 Shutter-SupportsTriggering": "0", "COM1-Description": "Serial port driver (boost:asio)", "PRIME-MetadataEnabled": "No", "520 Shutter-MinVolts": "0.0000", "470 Shutter-Sequenceable": "1", "PRIME-PreampDelay": "0", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-MinVolts": "0.0000", "ChannelIndex": 2, "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Core-AutoShutter": "1", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "Exposure-ms": 320, "PRIME-PP 2 ENABLED": "Yes", "640 Shutter-TriggerSampleRate": "100000", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "470 Shutter-IOChannel": "Dev1/ao10", "405 AO-Demo": "No", "PixelSizeUm": 1.1, "470 AO-Demo": "No", "470 Shutter-MaxVolts": "5.0000", "Vincent-D3-Name": "Vincent-D3", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FileName": "img_000000000_Il-A_010.tif", "LudlController-Reset": "Operate", "PRIME-Exposure": "320.00", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "445-Name": "DA Shutter", "555 Shutter-Name": "AnalogIO", "520 Shutter-TriggerSampleRate": "100000", "445 Shutter-IOChannel": "Dev1/ao9", "PRIME-TransposeCorrection": "0", "XYStage-StepSize": "0.1000", "445-Description": "DA device that is used as a shutter", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 AO-IOChannel": "Dev1/ao7", "520 Shutter-Name": "AnalogIO", "COM3-DataBits": "8", "640 AO-Volts": "4.5000", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "555 Shutter-IOChannel": "Dev1/ao13", "WaitInterval": null, "520 Shutter-Sequenceable": "1", "YPositionUm": 31506.2, "445-State": "0", "445-DA Device": "445 Shutter", "PRIME-CircularBufferAutoSize": "ON", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "555 AO-TriggerSampleRate": "100000", "445 Shutter-Volts": "0.0000", "XLIGHT Hub-Port": "COM3", "Core-AutoFocus": "", "COM4-Handshaking": "Off", "528 Shutter-MinVolts": "0.0000", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "445 Shutter-Name": "AnalogIO", "528 Shutter-Sequenceable": "1", "XYStage-TransposeMirrorX": "0", "XYStage-TransposeMirrorY": "0", "PRIME-ReadoutRate": "100MHz 16bit", "XYStage-Name": "Stage", "640 AO-SupportsTriggering": "0", "640-Name": "DA Shutter", "640 Shutter-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "640 AO-Block voltage": "false", "445 Shutter-Sequenceable": "1", "470 AO-MaxVolts": "5.0000", "COM4-BaudRate": "9600", "470-Description": "DA device that is used as a shutter", "520 Shutter-Description": "NI DAC", "PRIME-CCDTemperature": "-14.8500", "Time": "2019-12-18 21:37:00 -0500", "640 AO-TriggerSequenceLength": "1000", "470 AO-Name": "AnalogIO", "XLIGHT Disk slider-Label": "Disk Pos 40um", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "Binning": "1x1", "TE2000-ModelType": "1", "COM3-AnswerTimeout": "500.0000", "555 Shutter-SupportsTriggering": "0", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "TE2000-Port": "COM4", "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Description": "NI DAC", "COM4-AnswerTimeout": "500.0000", "COM1-DelayBetweenCharsMs": "0.0000", "FilterBlock-Label": "Confocal", "445 AO-Name": "AnalogIO", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-Timing-ReadoutTimeNs": "24672000.0000", "528 AO-Demo": "No", "520 AO-Sequenceable": "1", "555-Description": "DA device that is used as a shutter", "405-DA Device": "405 Shutter", "405 AO-TriggerSequenceLength": "1000", "640 AO-MinVolts": "0.0000", "640 Shutter-Block voltage": "false", "FrameIndex": 0, "PRIME-Port": "CMOS", "555 AO-MinVolts": "0.0000", "COM3-Description": "Serial port driver (boost:asio)", "528 AO-Description": "NI DAC", "555 AO-IOChannel": "Dev1/ao6", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "555 AO-TriggerInputLine": "/Dev1/PFI0", "PRIME-ActualInterval-ms": "390.5200", "LudlController-Port": "COM9", "445 Shutter-Demo": "No", "PRIME-Trigger-Expose Out-Mux": "1", "SliceIndex": 10, "PRIME-CCDTemperatureSetPoint": "-15.0000", "405 Shutter-MinVolts": "0.0000", "PRIME-ExposeOutMode": "First Row", "AxisPositions": null, "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Volts": "0.0000", "528-Name": "DA Shutter", "555-State": "0", "XLIGHT Dichroic-Label": "Di03-R635-t1", "445 AO-Sequenceable": "1", "405-Name": "DA Shutter", "405 AO-IOChannel": "Dev1/ao0", "Core-Camera": "PRIME", "Focus-Name": "Focus", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "405 Shutter-IOChannel": "Dev1/ao8", "640 AO-Demo": "No", "640 Shutter-SupportsTriggering": "0", "445 AO-Demo": "No", "555-DA Device": "555 Shutter", "470 Shutter-Description": "NI DAC", "528 AO-Volts": "0.0000", "COM1-DataBits": "8", "Vincent-D3-Address": "x", "520 AO-SupportsTriggering": "0", "405-Description": "DA device that is used as a shutter", "640 Shutter-MinVolts": "0.0000", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "405 AO-Name": "AnalogIO", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "640 AO-TriggerInputLine": "/Dev1/PFI0", "405 AO-TriggerInputLine": "/Dev1/PFI0", "640-Description": "DA device that is used as a shutter", "445 Shutter-TriggerSampleRate": "100000", "470 Shutter-TriggerSequenceLength": "1000", "COM1-StopBits": "1", "520 Shutter-SupportsTriggering": "0", "405-State": "0", "Core-Initialize": "1", "XLIGHT Emission Wheel-State": "3", "520 AO-Description": "NI DAC", "528 AO-TriggerInputLine": "/Dev1/PFI0", "445 Shutter-MaxVolts": "5.0000", "COM9-Handshaking": "Off", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "XLIGHT Spin motor-Label": "On (spinning)", "PRIME-PP 5 ENABLED": "No", "UUID": "81c5d53c-b748-4aee-84b9-f3db115bcb3a", "PRIME-TriggerMode": "Internal Trigger", "COM1-Handshaking": "Off", "PRIME-Timing-PostTriggerDelayNs": "49344000.0000", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "Focus-Position": "17741.5000", "528 Shutter-Block voltage": "false", "Channel": "Il-A", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "555 Shutter-Demo": "No", "405 Shutter-Demo": "No", "528 AO-Name": "AnalogIO", "520-State": "0", "Core-Galvo": "", "405 Shutter-TriggerSampleRate": "100000", "Focus-FocusStepSize_(nm)": "100", "PRIME-PixelType": "16bit", "COM4-DataBits": "8", "470 AO-IOChannel": "Dev1/ao2", "520 AO-MinVolts": "0.0000", "COM9-Parity": "None", "PRIME-CircularBufferEnabled": "ON", "PRIME-PP 6 Reset": "No", "640 AO-Description": "NI DAC", "Source": "PRIME", "445 AO-MinVolts": "0.0000", "PIEZO-Name": "Stage", "445 AO-Description": "NI DAC", "PRIME-CircularBufferFrameCount": "46", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "520 AO-Volts": "0.0000", "528 Shutter-TriggerSampleRate": "100000", "XPositionUm": 19731.9, "OpticalPath-State": "4", "PRIME-PP 3 THRESHOLD": "80", "PRIME-PVCAM Version": "3.7.1", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Gain": "1-HDR", "640 Shutter-TriggerSequenceLength": "1000", "528 AO-SupportsTriggering": "0", "445 AO-TriggerInputLine": "/Dev1/PFI0", "OpticalPath-Name": "OpticalPath", "PRIME-PP 4 ENABLED": "Yes", "PRIME-PVCAM Adapter Version": "1.3.29", "COM3-Name": "COM3", "405 AO-MaxVolts": "5.0000", "520 Shutter-Block voltage": "false", "PRIME-Timing-ClearingTimeNs": "49344000.0000", "Core-Focus": "PIEZO", "528 AO-Block voltage": "false", "TE2000-Description": "Nikon TE2000 hub", "528 AO-MinVolts": "0.0000", "COM4-Parity": "None", "Summary": { "Slices": 11, "ChContrastMin": [ 0, 0, 0, 0, 0, 0 ], "PixelAspect": 1, "SlicesFirst": true, "Interval_ms": 0, "Time": "2019-12-18 21:36:42 -0500", "Prefix": "seq_4", "InitialPositionList": [ { "Label": "Pos0", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.21], "Focus": [17741.5], "XYStage": [ 44542.200000000004, 31636.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos1", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.22], "Focus": [17741.4], "XYStage": [ 43801.100000000006, 31637.9 ] }, "GridColumnIndex": 0 }, { "Label": "Pos2", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.5], "XYStage": [ 38561.3, 32040.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos3", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.185], "Focus": [17741.3], "XYStage": [ 37698.4, 32041.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos4", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.16], "Focus": [17741.5], "XYStage": [ 32375.4, 31758.9 ] }, "GridColumnIndex": 0 }, { "Label": "Pos5", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.17000000000002], "Focus": [17741.3], "XYStage": [ 31770.7, 31758.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos6", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.4], "XYStage": [ 26536.800000000003, 31068.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos7", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.205], "Focus": [17741.4], "XYStage": [ 25420.2, 31068.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos8", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.85], "Focus": [17741.3], "XYStage": [ 21059, 31508 ] }, "GridColumnIndex": 0 }, { "Label": "Pos9", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.82], "Focus": [17741.3], "XYStage": [ 19731.9, 31506.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos10", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.79], "Focus": [17741.4], "XYStage": [ 14528.5, 32007.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos11", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.83], "Focus": [17741.2], "XYStage": [ 13830.300000000001, 32009.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos12", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.79], "Focus": [17741.3], "XYStage": [ 8775.300000000001, 32017.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos13", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.815], "Focus": [17741.3], "XYStage": [ 8109.200000000001, 32008.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos14", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.805], "Focus": [17741.3], "XYStage": [ 3483.3, 31908.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos15", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.82], "Focus": [17741.2], "XYStage": [ 2686.8, 31903.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos16", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [211.48000000000002], "Focus": [17741.1], "XYStage": [ -1978.9, 31973.600000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos17", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [211.51500000000001], "Focus": [17741.1], "XYStage": [ -2957.8, 31982.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos18", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.195], "Focus": [17741.5], "XYStage": [ 47747.4, -576.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos19", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.23000000000002], "Focus": [17741.6], "XYStage": [ 47053.9, -576.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos20", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.205], "Focus": [17741.4], "XYStage": [ 41871.200000000004, -479.8 ] }, "GridColumnIndex": 0 }, { "Label": "Pos21", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.4], "XYStage": [ 40882.600000000006, -392 ] }, "GridColumnIndex": 0 }, { "Label": "Pos22", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.24], "Focus": [17741.4], "XYStage": [ 35905.6, -135.3 ] }, "GridColumnIndex": 0 }, { "Label": "Pos23", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.195], "Focus": [17741.5], "XYStage": [ 34990.9, -132.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos24", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.46], "Focus": [17741.4], "XYStage": [ 29678.100000000002, -958.1 ] }, "GridColumnIndex": 0 }, { "Label": "Pos25", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.44], "Focus": [17741.5], "XYStage": [ 28983.600000000002, -939.4000000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos26", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.475], "Focus": [17741.5], "XYStage": [ 23019.7, -122 ] }, "GridColumnIndex": 0 }, { "Label": "Pos27", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.44], "Focus": [17741.7], "XYStage": [ 22428, -116.80000000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos28", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.46], "Focus": [17741.7], "XYStage": [ 16364.900000000001, -181.10000000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos29", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.48000000000002], "Focus": [17741.5], "XYStage": [ 15712.1, -180 ] }, "GridColumnIndex": 0 }, { "Label": "Pos30", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.45000000000002], "Focus": [17741.7], "XYStage": [ 10346.1, 77.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos31", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.49], "Focus": [17741.4], "XYStage": [ 9755.5, 85.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos32", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.475], "Focus": [17741.5], "XYStage": [ 4775.5, 293.90000000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos33", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.445], "Focus": [17741.4], "XYStage": [ 3954.9, 293.90000000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos34", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.455], "Focus": [17741.4], "XYStage": [ -1324.9, 545.6 ] }, "GridColumnIndex": 0 }, { "Label": "Pos35", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.445], "Focus": [17741.3], "XYStage": [ -2097, 576.6 ] }, "GridColumnIndex": 0 } ], "z-step_um": 10, "GridRow": 0, "BitDepth": 16, "Depth": 2, "Channels": 6, "Source": "Micro-Manager", "ChColors": [ -16711681, -256, -65281, -1, -1, -16776961 ], "MetadataVersion": 10, "KeepShutterOpenChannels": true, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536, 65536 ], "CustomIntervals_ms": [], "Height": 1200, "MicroManagerVersion": "1.4.23 20180219", "GridColumn": 0, "UUID": "14a13bf7-c944-4672-b873-fcfbbb7ea13d", "PixelType": "GRAY16", "Width": 1200, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\Yan\\20191218-YC113-S7S8-miseq", "Frames": 1, "Comment": "", "UserName": "Nikon", "PixelSize_um": 1.1, "ROI": [ 0, 0, 1200, 1200 ], "Date": "2019-12-18", "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC", "P-DAPI-high" ], "KeepShutterOpenSlices": true, "IJType": 1, "ComputerName": "ZadorConfocal2", "Positions": 36, "PositionIndex": 9, "CameraTimeout": 20000, "TimeFirst": false }, "640 AO-Name": "AnalogIO", "Vincent-D3-Command": "Close" }, "FrameKey-0-3-0": { "COM4-StopBits": "1", "528 AO-MaxVolts": "5.0000", "XLIGHT Excitation Wheel-State": "1", "470 Shutter-Name": "AnalogIO", "Core-Shutter": "640", "555 AO-Demo": "No", "XLIGHT Dichroic-State": "2", "XYStage-StepSize-Y": "0.1000", "ZPositionUm": 198.82, "XYStage-StepSize-X": "0.1000", "PRIME-FirmwareVersion": "20.28", "PRIME-ClearCycles": "2", "520-Name": "DA Shutter", "405 Shutter-Name": "AnalogIO", "PIEZO-StepSize": "0.0050", "PRIME-ClearMode": "Pre-Exposure", "470 AO-MinVolts": "0.0000", "405 AO-SupportsTriggering": "1", "CameraChannelIndex": 0, "XYStage-StartSpeed": "500.0000", "COM9-StopBits": "2", "405 AO-Description": "NI DAC", "COM4-DelayBetweenCharsMs": "0.0000", "445 AO-Block voltage": "false", "520 AO-TriggerInputLine": "/Dev1/PFI0", "555 AO-Description": "NI DAC", "520 AO-TriggerSampleRate": "100000", "COM4-Verbose": "1", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM4-Name": "COM4", "405 Shutter-Volts": "0.0000", "Vincent-D3-Port": "COM1", "PRIME-PP 4 MIN ADU AFFECTED": "200", "470 Shutter-MinVolts": "0.0000", "PRIME-PP 3 ENABLED": "Yes", "470 AO-Description": "NI DAC", "PRIME-Description": "PVCAM API device adapter", "520 Shutter-MaxVolts": "5.0000", "520 Shutter-Volts": "0.0000", "528 AO-Sequenceable": "1", "PRIME-Binning": "1x1", "555 Shutter-MaxVolts": "5.0000", "528 Shutter-TriggerSequenceLength": "1000", "405 Shutter-Block voltage": "false", "XLIGHT Spin motor-State": "1", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "Vincent-D3-Time to open (ms)": "35.0000", "Vincent-D3-Time to close (ms)": "35.0000", "445 AO-TriggerSampleRate": "100000", "640 Shutter-IOChannel": "Dev1/ao14", "555 AO-Sequenceable": "1", "520 AO-Name": "AnalogIO", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "470 AO-Block voltage": "false", "COM1-Verbose": "1", "COM9-AnswerTimeout": "10000.0000", "528 Shutter-Name": "AnalogIO", "PRIME-Trigger Timeout (secs)": "10", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "NextFrame": 0, "470 AO-TriggerSequenceLength": "1000", "PRIME-TransposeXY": "0", "445 AO-TriggerSequenceLength": "1000", "COM1-Name": "COM1", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "COM9-DataBits": "8", "COM1-AnswerTimeout": "500.0000", "Camera": "", "TE2000-Version": "V2.50-V2.50-V2.10", "528 Shutter-Volts": "0.0000", "XLIGHT Hub-Name": "XLIGHT Hub", "PIEZO-Description": "Ludl stage driver adapter", "PositionName": "Pos9", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSampleRate": "100000", "PRIME-FanSpeedSetpoint": "High", "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "405 AO-TriggerSampleRate": "100000", "470 Shutter-Block voltage": "false", "470-State": "0", "COM3-Parity": "None", "405 AO-Volts": "0.0000", "PRIME-TransposeMirrorY": "0", "PRIME-ChipName": "GS144BSI", "470 AO-Volts": "0.0000", "PRIME-TransposeMirrorX": "0", "445 Shutter-SupportsTriggering": "0", "470 Shutter-SupportsTriggering": "0", "XLIGHT Hub-Description": "XLIGHT hub", "PRIME-PreampOffLimit": "10000", "470 AO-Sequenceable": "1", "528 Shutter-MaxVolts": "5.0000", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "Core-XYStage": "XYStage", "470 AO-TriggerSampleRate": "100000", "PRIME-Timing-PreTriggerDelayNs": "24672000.0000", "528-DA Device": "528 Shutter", "Focus-Description": "Nikon TE2000 focus adapter", "FilterBlock-State": "5", "520 AO-Demo": "No", "COM3-DelayBetweenCharsMs": "0.0000", "470 Shutter-Volts": "0.0000", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "PRIME-PP 2 THRESHOLD": "125", "PRIME-PP 4 THRESHOLD": "75", "XYStage-Acceleration": "4.0000", "470 Shutter-Demo": "No", "PRIME-Name": "PMPCIECam00", "640-DA Device": "640 Shutter", "405 AO-Block voltage": "false", "ElapsedTime-ms": 393733, "PRIME-Offset": "100", "445 AO-IOChannel": "Dev1/ao1", "640 AO-MaxVolts": "5.0000", "PositionIndex": 9, "PRIME-PP 1 ENABLED": "Yes", "COM1-BaudRate": "9600", "640-State": "1", "COM3-Verbose": "1", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "LudlController-TransmissionDelay": "0", "PRIME-FullWellCapacity": "62000", "PRIME-SMARTStreamingEnabled": "No", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "640 Shutter-Description": "NI DAC", "528-Description": "DA device that is used as a shutter", "COM4-Description": "Serial port driver (boost:asio)", "405 Shutter-Description": "NI DAC", "TE2000-Name": "TE2000", "528 Shutter-Description": "NI DAC", "COM9-Description": "Serial port driver (boost:asio)", "LudlController-Name": "LudlController", "445 Shutter-TriggerSequenceLength": "1000", "PRIME-SerialNumber": "A18B203008", "445 AO-Volts": "0.0000", "PRIME-X-dimension": "1200", "PRIME-ShutterCloseDelay": "0", "XLIGHT Disk slider-State": "2", "Position": "Default", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 AO-IOChannel": "Dev1/ao5", "520 AO-IOChannel": "Dev1/ao4", "528 AO-TriggerSequenceLength": "1000", "405 Shutter-MaxVolts": "5.0000", "470-DA Device": "470 Shutter", "PRIME-FTCapable": "No", "Core-ChannelGroup": "Channels", "555 AO-SupportsTriggering": "0", "PRIME-Y-dimension": "1200", "Frame": 0, "555 Shutter-Sequenceable": "1", "PRIME-Timing-ExposureTimeNs": "499998000.0000", "Core-ImageProcessor": "", "528-State": "0", "520 AO-Block voltage": "false", "XLIGHT Excitation Wheel-Label": "QUAD", "640 Shutter-Demo": "No", "COM3-StopBits": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "520 Shutter-IOChannel": "Dev1/ao11", "Width": 1200, "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-SupportsTriggering": "0", "640 AO-TriggerSampleRate": "100000", "520-Description": "DA device that is used as a shutter", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "405 Shutter-Sequenceable": "1", "405 Shutter-SupportsTriggering": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-TriggerSampleRate": "100000", "640 Shutter-MaxVolts": "5.0000", "555 AO-MaxVolts": "5.0000", "520 AO-TriggerSequenceLength": "1000", "COM3-BaudRate": "9600", "COM9-BaudRate": "9600", "Vincent-D3-Channel #": "Ch. #1", "PRIME-PMode": "Normal", "OpticalPath-Label": "Left(100)", "BitDepth": 16, "555 AO-TriggerSequenceLength": "1000", "445 Shutter-Description": "NI DAC", "405 AO-Sequenceable": "1", "PRIME-AcquisitionMethod": "Callbacks", "555 AO-Name": "AnalogIO", "PRIME-ShutterMode": "Pre-Exposure", "COM3-Handshaking": "Off", "445 AO-SupportsTriggering": "0", "ROI": "0-0-1200-1200", "COM9-Verbose": "1", "PRIME-Color": "OFF", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "PRIME-ShutterOpenDelay": "0", "COM1-Parity": "None", "Slice": 0, "SlicePosition": -50, "COM9-DelayBetweenCharsMs": "0.0000", "555-Name": "DA Shutter", "PIEZO-LudlSingleAxisName": "B", "XLIGHT Emission Wheel-Label": "725/40 (C)", "640 Shutter-Name": "AnalogIO", "640 AO-Sequenceable": "1", "520 Shutter-Demo": "No", "528 Shutter-Demo": "No", "470-Name": "DA Shutter", "520-DA Device": "520 Shutter", "XYStage-Description": "Ludl XY stage driver adapter", "445 Shutter-MinVolts": "0.0000", "528 AO-TriggerSampleRate": "100000", "405 AO-MinVolts": "0.0000", "405 Shutter-TriggerSequenceLength": "1000", "Height": 1200, "555 AO-Block voltage": "false", "640 Shutter-Volts": "5.0000", "PixelType": "GRAY16", "445 Shutter-Block voltage": "false", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "520 Shutter-TriggerSequenceLength": "1000", "COM9-Name": "COM9", "Core-SLM": "", "555 AO-Volts": "0.0000", "555 Shutter-Block voltage": "false", "XYStage-Speed": "1800.0000", "520 AO-MaxVolts": "5.0000", "528 Shutter-SupportsTriggering": "0", "COM1-Description": "Serial port driver (boost:asio)", "PRIME-MetadataEnabled": "No", "520 Shutter-MinVolts": "0.0000", "470 Shutter-Sequenceable": "1", "PRIME-PreampDelay": "0", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-MinVolts": "0.0000", "ChannelIndex": 3, "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Core-AutoShutter": "1", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "Exposure-ms": 520, "PRIME-PP 2 ENABLED": "Yes", "640 Shutter-TriggerSampleRate": "100000", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "470 Shutter-IOChannel": "Dev1/ao10", "405 AO-Demo": "No", "PixelSizeUm": 1.1, "470 AO-Demo": "No", "470 Shutter-MaxVolts": "5.0000", "Vincent-D3-Name": "Vincent-D3", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FileName": "img_000000000_Il-C_000.tif", "LudlController-Reset": "Operate", "PRIME-Exposure": "520.00", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "445-Name": "DA Shutter", "555 Shutter-Name": "AnalogIO", "520 Shutter-TriggerSampleRate": "100000", "445 Shutter-IOChannel": "Dev1/ao9", "PRIME-TransposeCorrection": "0", "XYStage-StepSize": "0.1000", "445-Description": "DA device that is used as a shutter", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 AO-IOChannel": "Dev1/ao7", "520 Shutter-Name": "AnalogIO", "COM3-DataBits": "8", "640 AO-Volts": "4.5000", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "555 Shutter-IOChannel": "Dev1/ao13", "WaitInterval": null, "520 Shutter-Sequenceable": "1", "YPositionUm": 31506.2, "445-State": "0", "445-DA Device": "445 Shutter", "PRIME-CircularBufferAutoSize": "ON", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "555 AO-TriggerSampleRate": "100000", "445 Shutter-Volts": "0.0000", "XLIGHT Hub-Port": "COM3", "Core-AutoFocus": "", "COM4-Handshaking": "Off", "528 Shutter-MinVolts": "0.0000", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "445 Shutter-Name": "AnalogIO", "528 Shutter-Sequenceable": "1", "XYStage-TransposeMirrorX": "0", "XYStage-TransposeMirrorY": "0", "PRIME-ReadoutRate": "100MHz 16bit", "XYStage-Name": "Stage", "640 AO-SupportsTriggering": "0", "640-Name": "DA Shutter", "640 Shutter-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "640 AO-Block voltage": "false", "445 Shutter-Sequenceable": "1", "470 AO-MaxVolts": "5.0000", "COM4-BaudRate": "9600", "470-Description": "DA device that is used as a shutter", "520 Shutter-Description": "NI DAC", "PRIME-CCDTemperature": "-14.8500", "Time": "2019-12-18 21:37:01 -0500", "640 AO-TriggerSequenceLength": "1000", "470 AO-Name": "AnalogIO", "XLIGHT Disk slider-Label": "Disk Pos 40um", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "Binning": "1x1", "TE2000-ModelType": "1", "COM3-AnswerTimeout": "500.0000", "555 Shutter-SupportsTriggering": "0", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "TE2000-Port": "COM4", "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Description": "NI DAC", "COM4-AnswerTimeout": "500.0000", "COM1-DelayBetweenCharsMs": "0.0000", "FilterBlock-Label": "Confocal", "445 AO-Name": "AnalogIO", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-Timing-ReadoutTimeNs": "24672000.0000", "528 AO-Demo": "No", "520 AO-Sequenceable": "1", "555-Description": "DA device that is used as a shutter", "405-DA Device": "405 Shutter", "405 AO-TriggerSequenceLength": "1000", "640 AO-MinVolts": "0.0000", "640 Shutter-Block voltage": "false", "FrameIndex": 0, "PRIME-Port": "CMOS", "555 AO-MinVolts": "0.0000", "COM3-Description": "Serial port driver (boost:asio)", "528 AO-Description": "NI DAC", "555 AO-IOChannel": "Dev1/ao6", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "555 AO-TriggerInputLine": "/Dev1/PFI0", "PRIME-ActualInterval-ms": "390.5200", "LudlController-Port": "COM9", "445 Shutter-Demo": "No", "PRIME-Trigger-Expose Out-Mux": "1", "SliceIndex": 0, "PRIME-CCDTemperatureSetPoint": "-15.0000", "405 Shutter-MinVolts": "0.0000", "PRIME-ExposeOutMode": "First Row", "AxisPositions": null, "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Volts": "0.0000", "528-Name": "DA Shutter", "555-State": "0", "XLIGHT Dichroic-Label": "Di03-R635-t1", "445 AO-Sequenceable": "1", "405-Name": "DA Shutter", "405 AO-IOChannel": "Dev1/ao0", "Core-Camera": "PRIME", "Focus-Name": "Focus", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "405 Shutter-IOChannel": "Dev1/ao8", "640 AO-Demo": "No", "640 Shutter-SupportsTriggering": "0", "445 AO-Demo": "No", "555-DA Device": "555 Shutter", "470 Shutter-Description": "NI DAC", "528 AO-Volts": "0.0000", "COM1-DataBits": "8", "Vincent-D3-Address": "x", "520 AO-SupportsTriggering": "0", "405-Description": "DA device that is used as a shutter", "640 Shutter-MinVolts": "0.0000", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "405 AO-Name": "AnalogIO", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "640 AO-TriggerInputLine": "/Dev1/PFI0", "405 AO-TriggerInputLine": "/Dev1/PFI0", "640-Description": "DA device that is used as a shutter", "445 Shutter-TriggerSampleRate": "100000", "470 Shutter-TriggerSequenceLength": "1000", "COM1-StopBits": "1", "520 Shutter-SupportsTriggering": "0", "405-State": "0", "Core-Initialize": "1", "XLIGHT Emission Wheel-State": "3", "520 AO-Description": "NI DAC", "528 AO-TriggerInputLine": "/Dev1/PFI0", "445 Shutter-MaxVolts": "5.0000", "COM9-Handshaking": "Off", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "XLIGHT Spin motor-Label": "On (spinning)", "PRIME-PP 5 ENABLED": "No", "UUID": "14cf9c5a-e892-456c-bd69-46a2daf9d618", "PRIME-TriggerMode": "Internal Trigger", "COM1-Handshaking": "Off", "PRIME-Timing-PostTriggerDelayNs": "49344000.0000", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "Focus-Position": "17741.5000", "528 Shutter-Block voltage": "false", "Channel": "Il-C", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "555 Shutter-Demo": "No", "405 Shutter-Demo": "No", "528 AO-Name": "AnalogIO", "520-State": "0", "Core-Galvo": "", "405 Shutter-TriggerSampleRate": "100000", "Focus-FocusStepSize_(nm)": "100", "PRIME-PixelType": "16bit", "COM4-DataBits": "8", "470 AO-IOChannel": "Dev1/ao2", "520 AO-MinVolts": "0.0000", "COM9-Parity": "None", "PRIME-CircularBufferEnabled": "ON", "PRIME-PP 6 Reset": "No", "640 AO-Description": "NI DAC", "Source": "PRIME", "445 AO-MinVolts": "0.0000", "PIEZO-Name": "Stage", "445 AO-Description": "NI DAC", "PRIME-CircularBufferFrameCount": "46", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "520 AO-Volts": "0.0000", "528 Shutter-TriggerSampleRate": "100000", "XPositionUm": 19731.9, "OpticalPath-State": "4", "PRIME-PP 3 THRESHOLD": "80", "PRIME-PVCAM Version": "3.7.1", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Gain": "1-HDR", "640 Shutter-TriggerSequenceLength": "1000", "528 AO-SupportsTriggering": "0", "445 AO-TriggerInputLine": "/Dev1/PFI0", "OpticalPath-Name": "OpticalPath", "PRIME-PP 4 ENABLED": "Yes", "PRIME-PVCAM Adapter Version": "1.3.29", "COM3-Name": "COM3", "405 AO-MaxVolts": "5.0000", "520 Shutter-Block voltage": "false", "PRIME-Timing-ClearingTimeNs": "49344000.0000", "Core-Focus": "PIEZO", "528 AO-Block voltage": "false", "TE2000-Description": "Nikon TE2000 hub", "528 AO-MinVolts": "0.0000", "COM4-Parity": "None", "Summary": { "Slices": 11, "ChContrastMin": [ 0, 0, 0, 0, 0, 0 ], "PixelAspect": 1, "SlicesFirst": true, "Interval_ms": 0, "Time": "2019-12-18 21:36:42 -0500", "Prefix": "seq_4", "InitialPositionList": [ { "Label": "Pos0", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.21], "Focus": [17741.5], "XYStage": [ 44542.200000000004, 31636.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos1", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.22], "Focus": [17741.4], "XYStage": [ 43801.100000000006, 31637.9 ] }, "GridColumnIndex": 0 }, { "Label": "Pos2", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.5], "XYStage": [ 38561.3, 32040.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos3", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.185], "Focus": [17741.3], "XYStage": [ 37698.4, 32041.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos4", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.16], "Focus": [17741.5], "XYStage": [ 32375.4, 31758.9 ] }, "GridColumnIndex": 0 }, { "Label": "Pos5", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.17000000000002], "Focus": [17741.3], "XYStage": [ 31770.7, 31758.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos6", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.4], "XYStage": [ 26536.800000000003, 31068.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos7", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.205], "Focus": [17741.4], "XYStage": [ 25420.2, 31068.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos8", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.85], "Focus": [17741.3], "XYStage": [ 21059, 31508 ] }, "GridColumnIndex": 0 }, { "Label": "Pos9", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.82], "Focus": [17741.3], "XYStage": [ 19731.9, 31506.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos10", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.79], "Focus": [17741.4], "XYStage": [ 14528.5, 32007.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos11", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.83], "Focus": [17741.2], "XYStage": [ 13830.300000000001, 32009.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos12", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.79], "Focus": [17741.3], "XYStage": [ 8775.300000000001, 32017.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos13", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.815], "Focus": [17741.3], "XYStage": [ 8109.200000000001, 32008.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos14", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.805], "Focus": [17741.3], "XYStage": [ 3483.3, 31908.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos15", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.82], "Focus": [17741.2], "XYStage": [ 2686.8, 31903.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos16", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [211.48000000000002], "Focus": [17741.1], "XYStage": [ -1978.9, 31973.600000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos17", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [211.51500000000001], "Focus": [17741.1], "XYStage": [ -2957.8, 31982.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos18", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.195], "Focus": [17741.5], "XYStage": [ 47747.4, -576.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos19", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.23000000000002], "Focus": [17741.6], "XYStage": [ 47053.9, -576.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos20", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.205], "Focus": [17741.4], "XYStage": [ 41871.200000000004, -479.8 ] }, "GridColumnIndex": 0 }, { "Label": "Pos21", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.4], "XYStage": [ 40882.600000000006, -392 ] }, "GridColumnIndex": 0 }, { "Label": "Pos22", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.24], "Focus": [17741.4], "XYStage": [ 35905.6, -135.3 ] }, "GridColumnIndex": 0 }, { "Label": "Pos23", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.195], "Focus": [17741.5], "XYStage": [ 34990.9, -132.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos24", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.46], "Focus": [17741.4], "XYStage": [ 29678.100000000002, -958.1 ] }, "GridColumnIndex": 0 }, { "Label": "Pos25", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.44], "Focus": [17741.5], "XYStage": [ 28983.600000000002, -939.4000000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos26", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.475], "Focus": [17741.5], "XYStage": [ 23019.7, -122 ] }, "GridColumnIndex": 0 }, { "Label": "Pos27", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.44], "Focus": [17741.7], "XYStage": [ 22428, -116.80000000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos28", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.46], "Focus": [17741.7], "XYStage": [ 16364.900000000001, -181.10000000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos29", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.48000000000002], "Focus": [17741.5], "XYStage": [ 15712.1, -180 ] }, "GridColumnIndex": 0 }, { "Label": "Pos30", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.45000000000002], "Focus": [17741.7], "XYStage": [ 10346.1, 77.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos31", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.49], "Focus": [17741.4], "XYStage": [ 9755.5, 85.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos32", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.475], "Focus": [17741.5], "XYStage": [ 4775.5, 293.90000000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos33", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.445], "Focus": [17741.4], "XYStage": [ 3954.9, 293.90000000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos34", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.455], "Focus": [17741.4], "XYStage": [ -1324.9, 545.6 ] }, "GridColumnIndex": 0 }, { "Label": "Pos35", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.445], "Focus": [17741.3], "XYStage": [ -2097, 576.6 ] }, "GridColumnIndex": 0 } ], "z-step_um": 10, "GridRow": 0, "BitDepth": 16, "Depth": 2, "Channels": 6, "Source": "Micro-Manager", "ChColors": [ -16711681, -256, -65281, -1, -1, -16776961 ], "MetadataVersion": 10, "KeepShutterOpenChannels": true, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536, 65536 ], "CustomIntervals_ms": [], "Height": 1200, "MicroManagerVersion": "1.4.23 20180219", "GridColumn": 0, "UUID": "14a13bf7-c944-4672-b873-fcfbbb7ea13d", "PixelType": "GRAY16", "Width": 1200, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\Yan\\20191218-YC113-S7S8-miseq", "Frames": 1, "Comment": "", "UserName": "Nikon", "PixelSize_um": 1.1, "ROI": [ 0, 0, 1200, 1200 ], "Date": "2019-12-18", "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC", "P-DAPI-high" ], "KeepShutterOpenSlices": true, "IJType": 1, "ComputerName": "ZadorConfocal2", "Positions": 36, "PositionIndex": 9, "CameraTimeout": 20000, "TimeFirst": false }, "640 AO-Name": "AnalogIO", "Vincent-D3-Command": "Close" }, "FrameKey-0-3-1": { "COM4-StopBits": "1", "528 AO-MaxVolts": "5.0000", "XLIGHT Excitation Wheel-State": "1", "470 Shutter-Name": "AnalogIO", "Core-Shutter": "640", "555 AO-Demo": "No", "XLIGHT Dichroic-State": "2", "XYStage-StepSize-Y": "0.1000", "ZPositionUm": 208.82, "XYStage-StepSize-X": "0.1000", "PRIME-FirmwareVersion": "20.28", "PRIME-ClearCycles": "2", "520-Name": "DA Shutter", "405 Shutter-Name": "AnalogIO", "PIEZO-StepSize": "0.0050", "PRIME-ClearMode": "Pre-Exposure", "470 AO-MinVolts": "0.0000", "405 AO-SupportsTriggering": "1", "CameraChannelIndex": 0, "XYStage-StartSpeed": "500.0000", "COM9-StopBits": "2", "405 AO-Description": "NI DAC", "COM4-DelayBetweenCharsMs": "0.0000", "445 AO-Block voltage": "false", "520 AO-TriggerInputLine": "/Dev1/PFI0", "555 AO-Description": "NI DAC", "520 AO-TriggerSampleRate": "100000", "COM4-Verbose": "1", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM4-Name": "COM4", "405 Shutter-Volts": "0.0000", "Vincent-D3-Port": "COM1", "PRIME-PP 4 MIN ADU AFFECTED": "200", "470 Shutter-MinVolts": "0.0000", "PRIME-PP 3 ENABLED": "Yes", "470 AO-Description": "NI DAC", "PRIME-Description": "PVCAM API device adapter", "520 Shutter-MaxVolts": "5.0000", "520 Shutter-Volts": "0.0000", "528 AO-Sequenceable": "1", "PRIME-Binning": "1x1", "555 Shutter-MaxVolts": "5.0000", "528 Shutter-TriggerSequenceLength": "1000", "405 Shutter-Block voltage": "false", "XLIGHT Spin motor-State": "1", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "Vincent-D3-Time to open (ms)": "35.0000", "Vincent-D3-Time to close (ms)": "35.0000", "445 AO-TriggerSampleRate": "100000", "640 Shutter-IOChannel": "Dev1/ao14", "555 AO-Sequenceable": "1", "520 AO-Name": "AnalogIO", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "470 AO-Block voltage": "false", "COM1-Verbose": "1", "COM9-AnswerTimeout": "10000.0000", "528 Shutter-Name": "AnalogIO", "PRIME-Trigger Timeout (secs)": "10", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "NextFrame": 0, "470 AO-TriggerSequenceLength": "1000", "PRIME-TransposeXY": "0", "445 AO-TriggerSequenceLength": "1000", "COM1-Name": "COM1", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "COM9-DataBits": "8", "COM1-AnswerTimeout": "500.0000", "Camera": "", "TE2000-Version": "V2.50-V2.50-V2.10", "528 Shutter-Volts": "0.0000", "XLIGHT Hub-Name": "XLIGHT Hub", "PIEZO-Description": "Ludl stage driver adapter", "PositionName": "Pos9", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSampleRate": "100000", "PRIME-FanSpeedSetpoint": "High", "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "405 AO-TriggerSampleRate": "100000", "470 Shutter-Block voltage": "false", "470-State": "0", "COM3-Parity": "None", "405 AO-Volts": "0.0000", "PRIME-TransposeMirrorY": "0", "PRIME-ChipName": "GS144BSI", "470 AO-Volts": "0.0000", "PRIME-TransposeMirrorX": "0", "445 Shutter-SupportsTriggering": "0", "470 Shutter-SupportsTriggering": "0", "XLIGHT Hub-Description": "XLIGHT hub", "PRIME-PreampOffLimit": "10000", "470 AO-Sequenceable": "1", "528 Shutter-MaxVolts": "5.0000", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "Core-XYStage": "XYStage", "470 AO-TriggerSampleRate": "100000", "PRIME-Timing-PreTriggerDelayNs": "24672000.0000", "528-DA Device": "528 Shutter", "Focus-Description": "Nikon TE2000 focus adapter", "FilterBlock-State": "5", "520 AO-Demo": "No", "COM3-DelayBetweenCharsMs": "0.0000", "470 Shutter-Volts": "0.0000", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "PRIME-PP 2 THRESHOLD": "125", "PRIME-PP 4 THRESHOLD": "75", "XYStage-Acceleration": "4.0000", "470 Shutter-Demo": "No", "PRIME-Name": "PMPCIECam00", "640-DA Device": "640 Shutter", "405 AO-Block voltage": "false", "ElapsedTime-ms": 394536, "PRIME-Offset": "100", "445 AO-IOChannel": "Dev1/ao1", "640 AO-MaxVolts": "5.0000", "PositionIndex": 9, "PRIME-PP 1 ENABLED": "Yes", "COM1-BaudRate": "9600", "640-State": "1", "COM3-Verbose": "1", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "LudlController-TransmissionDelay": "0", "PRIME-FullWellCapacity": "62000", "PRIME-SMARTStreamingEnabled": "No", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "640 Shutter-Description": "NI DAC", "528-Description": "DA device that is used as a shutter", "COM4-Description": "Serial port driver (boost:asio)", "405 Shutter-Description": "NI DAC", "TE2000-Name": "TE2000", "528 Shutter-Description": "NI DAC", "COM9-Description": "Serial port driver (boost:asio)", "LudlController-Name": "LudlController", "445 Shutter-TriggerSequenceLength": "1000", "PRIME-SerialNumber": "A18B203008", "445 AO-Volts": "0.0000", "PRIME-X-dimension": "1200", "PRIME-ShutterCloseDelay": "0", "XLIGHT Disk slider-State": "2", "Position": "Default", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 AO-IOChannel": "Dev1/ao5", "520 AO-IOChannel": "Dev1/ao4", "528 AO-TriggerSequenceLength": "1000", "405 Shutter-MaxVolts": "5.0000", "470-DA Device": "470 Shutter", "PRIME-FTCapable": "No", "Core-ChannelGroup": "Channels", "555 AO-SupportsTriggering": "0", "PRIME-Y-dimension": "1200", "Frame": 0, "555 Shutter-Sequenceable": "1", "PRIME-Timing-ExposureTimeNs": "499998000.0000", "Core-ImageProcessor": "", "528-State": "0", "520 AO-Block voltage": "false", "XLIGHT Excitation Wheel-Label": "QUAD", "640 Shutter-Demo": "No", "COM3-StopBits": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "520 Shutter-IOChannel": "Dev1/ao11", "Width": 1200, "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-SupportsTriggering": "0", "640 AO-TriggerSampleRate": "100000", "520-Description": "DA device that is used as a shutter", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "405 Shutter-Sequenceable": "1", "405 Shutter-SupportsTriggering": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-TriggerSampleRate": "100000", "640 Shutter-MaxVolts": "5.0000", "555 AO-MaxVolts": "5.0000", "520 AO-TriggerSequenceLength": "1000", "COM3-BaudRate": "9600", "COM9-BaudRate": "9600", "Vincent-D3-Channel #": "Ch. #1", "PRIME-PMode": "Normal", "OpticalPath-Label": "Left(100)", "BitDepth": 16, "555 AO-TriggerSequenceLength": "1000", "445 Shutter-Description": "NI DAC", "405 AO-Sequenceable": "1", "PRIME-AcquisitionMethod": "Callbacks", "555 AO-Name": "AnalogIO", "PRIME-ShutterMode": "Pre-Exposure", "COM3-Handshaking": "Off", "445 AO-SupportsTriggering": "0", "ROI": "0-0-1200-1200", "COM9-Verbose": "1", "PRIME-Color": "OFF", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "PRIME-ShutterOpenDelay": "0", "COM1-Parity": "None", "Slice": 1, "SlicePosition": -40, "COM9-DelayBetweenCharsMs": "0.0000", "555-Name": "DA Shutter", "PIEZO-LudlSingleAxisName": "B", "XLIGHT Emission Wheel-Label": "725/40 (C)", "640 Shutter-Name": "AnalogIO", "640 AO-Sequenceable": "1", "520 Shutter-Demo": "No", "528 Shutter-Demo": "No", "470-Name": "DA Shutter", "520-DA Device": "520 Shutter", "XYStage-Description": "Ludl XY stage driver adapter", "445 Shutter-MinVolts": "0.0000", "528 AO-TriggerSampleRate": "100000", "405 AO-MinVolts": "0.0000", "405 Shutter-TriggerSequenceLength": "1000", "Height": 1200, "555 AO-Block voltage": "false", "640 Shutter-Volts": "5.0000", "PixelType": "GRAY16", "445 Shutter-Block voltage": "false", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "520 Shutter-TriggerSequenceLength": "1000", "COM9-Name": "COM9", "Core-SLM": "", "555 AO-Volts": "0.0000", "555 Shutter-Block voltage": "false", "XYStage-Speed": "1800.0000", "520 AO-MaxVolts": "5.0000", "528 Shutter-SupportsTriggering": "0", "COM1-Description": "Serial port driver (boost:asio)", "PRIME-MetadataEnabled": "No", "520 Shutter-MinVolts": "0.0000", "470 Shutter-Sequenceable": "1", "PRIME-PreampDelay": "0", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-MinVolts": "0.0000", "ChannelIndex": 3, "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Core-AutoShutter": "1", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "Exposure-ms": 520, "PRIME-PP 2 ENABLED": "Yes", "640 Shutter-TriggerSampleRate": "100000", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "470 Shutter-IOChannel": "Dev1/ao10", "405 AO-Demo": "No", "PixelSizeUm": 1.1, "470 AO-Demo": "No", "470 Shutter-MaxVolts": "5.0000", "Vincent-D3-Name": "Vincent-D3", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FileName": "img_000000000_Il-C_001.tif", "LudlController-Reset": "Operate", "PRIME-Exposure": "520.00", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "445-Name": "DA Shutter", "555 Shutter-Name": "AnalogIO", "520 Shutter-TriggerSampleRate": "100000", "445 Shutter-IOChannel": "Dev1/ao9", "PRIME-TransposeCorrection": "0", "XYStage-StepSize": "0.1000", "445-Description": "DA device that is used as a shutter", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 AO-IOChannel": "Dev1/ao7", "520 Shutter-Name": "AnalogIO", "COM3-DataBits": "8", "640 AO-Volts": "4.5000", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "555 Shutter-IOChannel": "Dev1/ao13", "WaitInterval": null, "520 Shutter-Sequenceable": "1", "YPositionUm": 31506.2, "445-State": "0", "445-DA Device": "445 Shutter", "PRIME-CircularBufferAutoSize": "ON", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "555 AO-TriggerSampleRate": "100000", "445 Shutter-Volts": "0.0000", "XLIGHT Hub-Port": "COM3", "Core-AutoFocus": "", "COM4-Handshaking": "Off", "528 Shutter-MinVolts": "0.0000", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "445 Shutter-Name": "AnalogIO", "528 Shutter-Sequenceable": "1", "XYStage-TransposeMirrorX": "0", "XYStage-TransposeMirrorY": "0", "PRIME-ReadoutRate": "100MHz 16bit", "XYStage-Name": "Stage", "640 AO-SupportsTriggering": "0", "640-Name": "DA Shutter", "640 Shutter-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "640 AO-Block voltage": "false", "445 Shutter-Sequenceable": "1", "470 AO-MaxVolts": "5.0000", "COM4-BaudRate": "9600", "470-Description": "DA device that is used as a shutter", "520 Shutter-Description": "NI DAC", "PRIME-CCDTemperature": "-14.8500", "Time": "2019-12-18 21:37:02 -0500", "640 AO-TriggerSequenceLength": "1000", "470 AO-Name": "AnalogIO", "XLIGHT Disk slider-Label": "Disk Pos 40um", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "Binning": "1x1", "TE2000-ModelType": "1", "COM3-AnswerTimeout": "500.0000", "555 Shutter-SupportsTriggering": "0", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "TE2000-Port": "COM4", "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Description": "NI DAC", "COM4-AnswerTimeout": "500.0000", "COM1-DelayBetweenCharsMs": "0.0000", "FilterBlock-Label": "Confocal", "445 AO-Name": "AnalogIO", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-Timing-ReadoutTimeNs": "24672000.0000", "528 AO-Demo": "No", "520 AO-Sequenceable": "1", "555-Description": "DA device that is used as a shutter", "405-DA Device": "405 Shutter", "405 AO-TriggerSequenceLength": "1000", "640 AO-MinVolts": "0.0000", "640 Shutter-Block voltage": "false", "FrameIndex": 0, "PRIME-Port": "CMOS", "555 AO-MinVolts": "0.0000", "COM3-Description": "Serial port driver (boost:asio)", "528 AO-Description": "NI DAC", "555 AO-IOChannel": "Dev1/ao6", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "555 AO-TriggerInputLine": "/Dev1/PFI0", "PRIME-ActualInterval-ms": "390.5200", "LudlController-Port": "COM9", "445 Shutter-Demo": "No", "PRIME-Trigger-Expose Out-Mux": "1", "SliceIndex": 1, "PRIME-CCDTemperatureSetPoint": "-15.0000", "405 Shutter-MinVolts": "0.0000", "PRIME-ExposeOutMode": "First Row", "AxisPositions": null, "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Volts": "0.0000", "528-Name": "DA Shutter", "555-State": "0", "XLIGHT Dichroic-Label": "Di03-R635-t1", "445 AO-Sequenceable": "1", "405-Name": "DA Shutter", "405 AO-IOChannel": "Dev1/ao0", "Core-Camera": "PRIME", "Focus-Name": "Focus", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "405 Shutter-IOChannel": "Dev1/ao8", "640 AO-Demo": "No", "640 Shutter-SupportsTriggering": "0", "445 AO-Demo": "No", "555-DA Device": "555 Shutter", "470 Shutter-Description": "NI DAC", "528 AO-Volts": "0.0000", "COM1-DataBits": "8", "Vincent-D3-Address": "x", "520 AO-SupportsTriggering": "0", "405-Description": "DA device that is used as a shutter", "640 Shutter-MinVolts": "0.0000", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "405 AO-Name": "AnalogIO", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "640 AO-TriggerInputLine": "/Dev1/PFI0", "405 AO-TriggerInputLine": "/Dev1/PFI0", "640-Description": "DA device that is used as a shutter", "445 Shutter-TriggerSampleRate": "100000", "470 Shutter-TriggerSequenceLength": "1000", "COM1-StopBits": "1", "520 Shutter-SupportsTriggering": "0", "405-State": "0", "Core-Initialize": "1", "XLIGHT Emission Wheel-State": "3", "520 AO-Description": "NI DAC", "528 AO-TriggerInputLine": "/Dev1/PFI0", "445 Shutter-MaxVolts": "5.0000", "COM9-Handshaking": "Off", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "XLIGHT Spin motor-Label": "On (spinning)", "PRIME-PP 5 ENABLED": "No", "UUID": "fabe0902-b4da-4d47-81fc-ea3e8ba799da", "PRIME-TriggerMode": "Internal Trigger", "COM1-Handshaking": "Off", "PRIME-Timing-PostTriggerDelayNs": "49344000.0000", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "Focus-Position": "17741.5000", "528 Shutter-Block voltage": "false", "Channel": "Il-C", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "555 Shutter-Demo": "No", "405 Shutter-Demo": "No", "528 AO-Name": "AnalogIO", "520-State": "0", "Core-Galvo": "", "405 Shutter-TriggerSampleRate": "100000", "Focus-FocusStepSize_(nm)": "100", "PRIME-PixelType": "16bit", "COM4-DataBits": "8", "470 AO-IOChannel": "Dev1/ao2", "520 AO-MinVolts": "0.0000", "COM9-Parity": "None", "PRIME-CircularBufferEnabled": "ON", "PRIME-PP 6 Reset": "No", "640 AO-Description": "NI DAC", "Source": "PRIME", "445 AO-MinVolts": "0.0000", "PIEZO-Name": "Stage", "445 AO-Description": "NI DAC", "PRIME-CircularBufferFrameCount": "46", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "520 AO-Volts": "0.0000", "528 Shutter-TriggerSampleRate": "100000", "XPositionUm": 19731.9, "OpticalPath-State": "4", "PRIME-PP 3 THRESHOLD": "80", "PRIME-PVCAM Version": "3.7.1", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Gain": "1-HDR", "640 Shutter-TriggerSequenceLength": "1000", "528 AO-SupportsTriggering": "0", "445 AO-TriggerInputLine": "/Dev1/PFI0", "OpticalPath-Name": "OpticalPath", "PRIME-PP 4 ENABLED": "Yes", "PRIME-PVCAM Adapter Version": "1.3.29", "COM3-Name": "COM3", "405 AO-MaxVolts": "5.0000", "520 Shutter-Block voltage": "false", "PRIME-Timing-ClearingTimeNs": "49344000.0000", "Core-Focus": "PIEZO", "528 AO-Block voltage": "false", "TE2000-Description": "Nikon TE2000 hub", "528 AO-MinVolts": "0.0000", "COM4-Parity": "None", "Summary": { "Slices": 11, "ChContrastMin": [ 0, 0, 0, 0, 0, 0 ], "PixelAspect": 1, "SlicesFirst": true, "Interval_ms": 0, "Time": "2019-12-18 21:36:42 -0500", "Prefix": "seq_4", "InitialPositionList": [ { "Label": "Pos0", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.21], "Focus": [17741.5], "XYStage": [ 44542.200000000004, 31636.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos1", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.22], "Focus": [17741.4], "XYStage": [ 43801.100000000006, 31637.9 ] }, "GridColumnIndex": 0 }, { "Label": "Pos2", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.5], "XYStage": [ 38561.3, 32040.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos3", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.185], "Focus": [17741.3], "XYStage": [ 37698.4, 32041.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos4", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.16], "Focus": [17741.5], "XYStage": [ 32375.4, 31758.9 ] }, "GridColumnIndex": 0 }, { "Label": "Pos5", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.17000000000002], "Focus": [17741.3], "XYStage": [ 31770.7, 31758.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos6", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.4], "XYStage": [ 26536.800000000003, 31068.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos7", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.205], "Focus": [17741.4], "XYStage": [ 25420.2, 31068.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos8", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.85], "Focus": [17741.3], "XYStage": [ 21059, 31508 ] }, "GridColumnIndex": 0 }, { "Label": "Pos9", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.82], "Focus": [17741.3], "XYStage": [ 19731.9, 31506.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos10", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.79], "Focus": [17741.4], "XYStage": [ 14528.5, 32007.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos11", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.83], "Focus": [17741.2], "XYStage": [ 13830.300000000001, 32009.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos12", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.79], "Focus": [17741.3], "XYStage": [ 8775.300000000001, 32017.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos13", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.815], "Focus": [17741.3], "XYStage": [ 8109.200000000001, 32008.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos14", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.805], "Focus": [17741.3], "XYStage": [ 3483.3, 31908.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos15", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.82], "Focus": [17741.2], "XYStage": [ 2686.8, 31903.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos16", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [211.48000000000002], "Focus": [17741.1], "XYStage": [ -1978.9, 31973.600000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos17", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [211.51500000000001], "Focus": [17741.1], "XYStage": [ -2957.8, 31982.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos18", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.195], "Focus": [17741.5], "XYStage": [ 47747.4, -576.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos19", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.23000000000002], "Focus": [17741.6], "XYStage": [ 47053.9, -576.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos20", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.205], "Focus": [17741.4], "XYStage": [ 41871.200000000004, -479.8 ] }, "GridColumnIndex": 0 }, { "Label": "Pos21", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.4], "XYStage": [ 40882.600000000006, -392 ] }, "GridColumnIndex": 0 }, { "Label": "Pos22", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.24], "Focus": [17741.4], "XYStage": [ 35905.6, -135.3 ] }, "GridColumnIndex": 0 }, { "Label": "Pos23", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.195], "Focus": [17741.5], "XYStage": [ 34990.9, -132.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos24", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.46], "Focus": [17741.4], "XYStage": [ 29678.100000000002, -958.1 ] }, "GridColumnIndex": 0 }, { "Label": "Pos25", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.44], "Focus": [17741.5], "XYStage": [ 28983.600000000002, -939.4000000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos26", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.475], "Focus": [17741.5], "XYStage": [ 23019.7, -122 ] }, "GridColumnIndex": 0 }, { "Label": "Pos27", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.44], "Focus": [17741.7], "XYStage": [ 22428, -116.80000000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos28", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.46], "Focus": [17741.7], "XYStage": [ 16364.900000000001, -181.10000000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos29", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.48000000000002], "Focus": [17741.5], "XYStage": [ 15712.1, -180 ] }, "GridColumnIndex": 0 }, { "Label": "Pos30", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.45000000000002], "Focus": [17741.7], "XYStage": [ 10346.1, 77.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos31", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.49], "Focus": [17741.4], "XYStage": [ 9755.5, 85.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos32", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.475], "Focus": [17741.5], "XYStage": [ 4775.5, 293.90000000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos33", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.445], "Focus": [17741.4], "XYStage": [ 3954.9, 293.90000000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos34", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.455], "Focus": [17741.4], "XYStage": [ -1324.9, 545.6 ] }, "GridColumnIndex": 0 }, { "Label": "Pos35", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.445], "Focus": [17741.3], "XYStage": [ -2097, 576.6 ] }, "GridColumnIndex": 0 } ], "z-step_um": 10, "GridRow": 0, "BitDepth": 16, "Depth": 2, "Channels": 6, "Source": "Micro-Manager", "ChColors": [ -16711681, -256, -65281, -1, -1, -16776961 ], "MetadataVersion": 10, "KeepShutterOpenChannels": true, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536, 65536 ], "CustomIntervals_ms": [], "Height": 1200, "MicroManagerVersion": "1.4.23 20180219", "GridColumn": 0, "UUID": "14a13bf7-c944-4672-b873-fcfbbb7ea13d", "PixelType": "GRAY16", "Width": 1200, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\Yan\\20191218-YC113-S7S8-miseq", "Frames": 1, "Comment": "", "UserName": "Nikon", "PixelSize_um": 1.1, "ROI": [ 0, 0, 1200, 1200 ], "Date": "2019-12-18", "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC", "P-DAPI-high" ], "KeepShutterOpenSlices": true, "IJType": 1, "ComputerName": "ZadorConfocal2", "Positions": 36, "PositionIndex": 9, "CameraTimeout": 20000, "TimeFirst": false }, "640 AO-Name": "AnalogIO", "Vincent-D3-Command": "Close" }, "FrameKey-0-3-2": { "COM4-StopBits": "1", "528 AO-MaxVolts": "5.0000", "XLIGHT Excitation Wheel-State": "1", "470 Shutter-Name": "AnalogIO", "Core-Shutter": "640", "555 AO-Demo": "No", "XLIGHT Dichroic-State": "2", "XYStage-StepSize-Y": "0.1000", "ZPositionUm": 218.82, "XYStage-StepSize-X": "0.1000", "PRIME-FirmwareVersion": "20.28", "PRIME-ClearCycles": "2", "520-Name": "DA Shutter", "405 Shutter-Name": "AnalogIO", "PIEZO-StepSize": "0.0050", "PRIME-ClearMode": "Pre-Exposure", "470 AO-MinVolts": "0.0000", "405 AO-SupportsTriggering": "1", "CameraChannelIndex": 0, "XYStage-StartSpeed": "500.0000", "COM9-StopBits": "2", "405 AO-Description": "NI DAC", "COM4-DelayBetweenCharsMs": "0.0000", "445 AO-Block voltage": "false", "520 AO-TriggerInputLine": "/Dev1/PFI0", "555 AO-Description": "NI DAC", "520 AO-TriggerSampleRate": "100000", "COM4-Verbose": "1", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM4-Name": "COM4", "405 Shutter-Volts": "0.0000", "Vincent-D3-Port": "COM1", "PRIME-PP 4 MIN ADU AFFECTED": "200", "470 Shutter-MinVolts": "0.0000", "PRIME-PP 3 ENABLED": "Yes", "470 AO-Description": "NI DAC", "PRIME-Description": "PVCAM API device adapter", "520 Shutter-MaxVolts": "5.0000", "520 Shutter-Volts": "0.0000", "528 AO-Sequenceable": "1", "PRIME-Binning": "1x1", "555 Shutter-MaxVolts": "5.0000", "528 Shutter-TriggerSequenceLength": "1000", "405 Shutter-Block voltage": "false", "XLIGHT Spin motor-State": "1", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "Vincent-D3-Time to open (ms)": "35.0000", "Vincent-D3-Time to close (ms)": "35.0000", "445 AO-TriggerSampleRate": "100000", "640 Shutter-IOChannel": "Dev1/ao14", "555 AO-Sequenceable": "1", "520 AO-Name": "AnalogIO", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "470 AO-Block voltage": "false", "COM1-Verbose": "1", "COM9-AnswerTimeout": "10000.0000", "528 Shutter-Name": "AnalogIO", "PRIME-Trigger Timeout (secs)": "10", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "NextFrame": 0, "470 AO-TriggerSequenceLength": "1000", "PRIME-TransposeXY": "0", "445 AO-TriggerSequenceLength": "1000", "COM1-Name": "COM1", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "COM9-DataBits": "8", "COM1-AnswerTimeout": "500.0000", "Camera": "", "TE2000-Version": "V2.50-V2.50-V2.10", "528 Shutter-Volts": "0.0000", "XLIGHT Hub-Name": "XLIGHT Hub", "PIEZO-Description": "Ludl stage driver adapter", "PositionName": "Pos9", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSampleRate": "100000", "PRIME-FanSpeedSetpoint": "High", "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "405 AO-TriggerSampleRate": "100000", "470 Shutter-Block voltage": "false", "470-State": "0", "COM3-Parity": "None", "405 AO-Volts": "0.0000", "PRIME-TransposeMirrorY": "0", "PRIME-ChipName": "GS144BSI", "470 AO-Volts": "0.0000", "PRIME-TransposeMirrorX": "0", "445 Shutter-SupportsTriggering": "0", "470 Shutter-SupportsTriggering": "0", "XLIGHT Hub-Description": "XLIGHT hub", "PRIME-PreampOffLimit": "10000", "470 AO-Sequenceable": "1", "528 Shutter-MaxVolts": "5.0000", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "Core-XYStage": "XYStage", "470 AO-TriggerSampleRate": "100000", "PRIME-Timing-PreTriggerDelayNs": "24672000.0000", "528-DA Device": "528 Shutter", "Focus-Description": "Nikon TE2000 focus adapter", "FilterBlock-State": "5", "520 AO-Demo": "No", "COM3-DelayBetweenCharsMs": "0.0000", "470 Shutter-Volts": "0.0000", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "PRIME-PP 2 THRESHOLD": "125", "PRIME-PP 4 THRESHOLD": "75", "XYStage-Acceleration": "4.0000", "470 Shutter-Demo": "No", "PRIME-Name": "PMPCIECam00", "640-DA Device": "640 Shutter", "405 AO-Block voltage": "false", "ElapsedTime-ms": 395300, "PRIME-Offset": "100", "445 AO-IOChannel": "Dev1/ao1", "640 AO-MaxVolts": "5.0000", "PositionIndex": 9, "PRIME-PP 1 ENABLED": "Yes", "COM1-BaudRate": "9600", "640-State": "1", "COM3-Verbose": "1", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "LudlController-TransmissionDelay": "0", "PRIME-FullWellCapacity": "62000", "PRIME-SMARTStreamingEnabled": "No", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "640 Shutter-Description": "NI DAC", "528-Description": "DA device that is used as a shutter", "COM4-Description": "Serial port driver (boost:asio)", "405 Shutter-Description": "NI DAC", "TE2000-Name": "TE2000", "528 Shutter-Description": "NI DAC", "COM9-Description": "Serial port driver (boost:asio)", "LudlController-Name": "LudlController", "445 Shutter-TriggerSequenceLength": "1000", "PRIME-SerialNumber": "A18B203008", "445 AO-Volts": "0.0000", "PRIME-X-dimension": "1200", "PRIME-ShutterCloseDelay": "0", "XLIGHT Disk slider-State": "2", "Position": "Default", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 AO-IOChannel": "Dev1/ao5", "520 AO-IOChannel": "Dev1/ao4", "528 AO-TriggerSequenceLength": "1000", "405 Shutter-MaxVolts": "5.0000", "470-DA Device": "470 Shutter", "PRIME-FTCapable": "No", "Core-ChannelGroup": "Channels", "555 AO-SupportsTriggering": "0", "PRIME-Y-dimension": "1200", "Frame": 0, "555 Shutter-Sequenceable": "1", "PRIME-Timing-ExposureTimeNs": "499998000.0000", "Core-ImageProcessor": "", "528-State": "0", "520 AO-Block voltage": "false", "XLIGHT Excitation Wheel-Label": "QUAD", "640 Shutter-Demo": "No", "COM3-StopBits": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "520 Shutter-IOChannel": "Dev1/ao11", "Width": 1200, "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-SupportsTriggering": "0", "640 AO-TriggerSampleRate": "100000", "520-Description": "DA device that is used as a shutter", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "405 Shutter-Sequenceable": "1", "405 Shutter-SupportsTriggering": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-TriggerSampleRate": "100000", "640 Shutter-MaxVolts": "5.0000", "555 AO-MaxVolts": "5.0000", "520 AO-TriggerSequenceLength": "1000", "COM3-BaudRate": "9600", "COM9-BaudRate": "9600", "Vincent-D3-Channel #": "Ch. #1", "PRIME-PMode": "Normal", "OpticalPath-Label": "Left(100)", "BitDepth": 16, "555 AO-TriggerSequenceLength": "1000", "445 Shutter-Description": "NI DAC", "405 AO-Sequenceable": "1", "PRIME-AcquisitionMethod": "Callbacks", "555 AO-Name": "AnalogIO", "PRIME-ShutterMode": "Pre-Exposure", "COM3-Handshaking": "Off", "445 AO-SupportsTriggering": "0", "ROI": "0-0-1200-1200", "COM9-Verbose": "1", "PRIME-Color": "OFF", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "PRIME-ShutterOpenDelay": "0", "COM1-Parity": "None", "Slice": 2, "SlicePosition": -30, "COM9-DelayBetweenCharsMs": "0.0000", "555-Name": "DA Shutter", "PIEZO-LudlSingleAxisName": "B", "XLIGHT Emission Wheel-Label": "725/40 (C)", "640 Shutter-Name": "AnalogIO", "640 AO-Sequenceable": "1", "520 Shutter-Demo": "No", "528 Shutter-Demo": "No", "470-Name": "DA Shutter", "520-DA Device": "520 Shutter", "XYStage-Description": "Ludl XY stage driver adapter", "445 Shutter-MinVolts": "0.0000", "528 AO-TriggerSampleRate": "100000", "405 AO-MinVolts": "0.0000", "405 Shutter-TriggerSequenceLength": "1000", "Height": 1200, "555 AO-Block voltage": "false", "640 Shutter-Volts": "5.0000", "PixelType": "GRAY16", "445 Shutter-Block voltage": "false", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "520 Shutter-TriggerSequenceLength": "1000", "COM9-Name": "COM9", "Core-SLM": "", "555 AO-Volts": "0.0000", "555 Shutter-Block voltage": "false", "XYStage-Speed": "1800.0000", "520 AO-MaxVolts": "5.0000", "528 Shutter-SupportsTriggering": "0", "COM1-Description": "Serial port driver (boost:asio)", "PRIME-MetadataEnabled": "No", "520 Shutter-MinVolts": "0.0000", "470 Shutter-Sequenceable": "1", "PRIME-PreampDelay": "0", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-MinVolts": "0.0000", "ChannelIndex": 3, "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Core-AutoShutter": "1", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "Exposure-ms": 520, "PRIME-PP 2 ENABLED": "Yes", "640 Shutter-TriggerSampleRate": "100000", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "470 Shutter-IOChannel": "Dev1/ao10", "405 AO-Demo": "No", "PixelSizeUm": 1.1, "470 AO-Demo": "No", "470 Shutter-MaxVolts": "5.0000", "Vincent-D3-Name": "Vincent-D3", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FileName": "img_000000000_Il-C_002.tif", "LudlController-Reset": "Operate", "PRIME-Exposure": "520.00", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "445-Name": "DA Shutter", "555 Shutter-Name": "AnalogIO", "520 Shutter-TriggerSampleRate": "100000", "445 Shutter-IOChannel": "Dev1/ao9", "PRIME-TransposeCorrection": "0", "XYStage-StepSize": "0.1000", "445-Description": "DA device that is used as a shutter", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 AO-IOChannel": "Dev1/ao7", "520 Shutter-Name": "AnalogIO", "COM3-DataBits": "8", "640 AO-Volts": "4.5000", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "555 Shutter-IOChannel": "Dev1/ao13", "WaitInterval": null, "520 Shutter-Sequenceable": "1", "YPositionUm": 31506.2, "445-State": "0", "445-DA Device": "445 Shutter", "PRIME-CircularBufferAutoSize": "ON", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "555 AO-TriggerSampleRate": "100000", "445 Shutter-Volts": "0.0000", "XLIGHT Hub-Port": "COM3", "Core-AutoFocus": "", "COM4-Handshaking": "Off", "528 Shutter-MinVolts": "0.0000", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "445 Shutter-Name": "AnalogIO", "528 Shutter-Sequenceable": "1", "XYStage-TransposeMirrorX": "0", "XYStage-TransposeMirrorY": "0", "PRIME-ReadoutRate": "100MHz 16bit", "XYStage-Name": "Stage", "640 AO-SupportsTriggering": "0", "640-Name": "DA Shutter", "640 Shutter-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "640 AO-Block voltage": "false", "445 Shutter-Sequenceable": "1", "470 AO-MaxVolts": "5.0000", "COM4-BaudRate": "9600", "470-Description": "DA device that is used as a shutter", "520 Shutter-Description": "NI DAC", "PRIME-CCDTemperature": "-14.8500", "Time": "2019-12-18 21:37:03 -0500", "640 AO-TriggerSequenceLength": "1000", "470 AO-Name": "AnalogIO", "XLIGHT Disk slider-Label": "Disk Pos 40um", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "Binning": "1x1", "TE2000-ModelType": "1", "COM3-AnswerTimeout": "500.0000", "555 Shutter-SupportsTriggering": "0", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "TE2000-Port": "COM4", "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Description": "NI DAC", "COM4-AnswerTimeout": "500.0000", "COM1-DelayBetweenCharsMs": "0.0000", "FilterBlock-Label": "Confocal", "445 AO-Name": "AnalogIO", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-Timing-ReadoutTimeNs": "24672000.0000", "528 AO-Demo": "No", "520 AO-Sequenceable": "1", "555-Description": "DA device that is used as a shutter", "405-DA Device": "405 Shutter", "405 AO-TriggerSequenceLength": "1000", "640 AO-MinVolts": "0.0000", "640 Shutter-Block voltage": "false", "FrameIndex": 0, "PRIME-Port": "CMOS", "555 AO-MinVolts": "0.0000", "COM3-Description": "Serial port driver (boost:asio)", "528 AO-Description": "NI DAC", "555 AO-IOChannel": "Dev1/ao6", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "555 AO-TriggerInputLine": "/Dev1/PFI0", "PRIME-ActualInterval-ms": "390.5200", "LudlController-Port": "COM9", "445 Shutter-Demo": "No", "PRIME-Trigger-Expose Out-Mux": "1", "SliceIndex": 2, "PRIME-CCDTemperatureSetPoint": "-15.0000", "405 Shutter-MinVolts": "0.0000", "PRIME-ExposeOutMode": "First Row", "AxisPositions": null, "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Volts": "0.0000", "528-Name": "DA Shutter", "555-State": "0", "XLIGHT Dichroic-Label": "Di03-R635-t1", "445 AO-Sequenceable": "1", "405-Name": "DA Shutter", "405 AO-IOChannel": "Dev1/ao0", "Core-Camera": "PRIME", "Focus-Name": "Focus", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "405 Shutter-IOChannel": "Dev1/ao8", "640 AO-Demo": "No", "640 Shutter-SupportsTriggering": "0", "445 AO-Demo": "No", "555-DA Device": "555 Shutter", "470 Shutter-Description": "NI DAC", "528 AO-Volts": "0.0000", "COM1-DataBits": "8", "Vincent-D3-Address": "x", "520 AO-SupportsTriggering": "0", "405-Description": "DA device that is used as a shutter", "640 Shutter-MinVolts": "0.0000", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "405 AO-Name": "AnalogIO", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "640 AO-TriggerInputLine": "/Dev1/PFI0", "405 AO-TriggerInputLine": "/Dev1/PFI0", "640-Description": "DA device that is used as a shutter", "445 Shutter-TriggerSampleRate": "100000", "470 Shutter-TriggerSequenceLength": "1000", "COM1-StopBits": "1", "520 Shutter-SupportsTriggering": "0", "405-State": "0", "Core-Initialize": "1", "XLIGHT Emission Wheel-State": "3", "520 AO-Description": "NI DAC", "528 AO-TriggerInputLine": "/Dev1/PFI0", "445 Shutter-MaxVolts": "5.0000", "COM9-Handshaking": "Off", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "XLIGHT Spin motor-Label": "On (spinning)", "PRIME-PP 5 ENABLED": "No", "UUID": "4d8bb8c0-23d6-4c6b-91aa-606aafc9f542", "PRIME-TriggerMode": "Internal Trigger", "COM1-Handshaking": "Off", "PRIME-Timing-PostTriggerDelayNs": "49344000.0000", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "Focus-Position": "17741.5000", "528 Shutter-Block voltage": "false", "Channel": "Il-C", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "555 Shutter-Demo": "No", "405 Shutter-Demo": "No", "528 AO-Name": "AnalogIO", "520-State": "0", "Core-Galvo": "", "405 Shutter-TriggerSampleRate": "100000", "Focus-FocusStepSize_(nm)": "100", "PRIME-PixelType": "16bit", "COM4-DataBits": "8", "470 AO-IOChannel": "Dev1/ao2", "520 AO-MinVolts": "0.0000", "COM9-Parity": "None", "PRIME-CircularBufferEnabled": "ON", "PRIME-PP 6 Reset": "No", "640 AO-Description": "NI DAC", "Source": "PRIME", "445 AO-MinVolts": "0.0000", "PIEZO-Name": "Stage", "445 AO-Description": "NI DAC", "PRIME-CircularBufferFrameCount": "46", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "520 AO-Volts": "0.0000", "528 Shutter-TriggerSampleRate": "100000", "XPositionUm": 19731.9, "OpticalPath-State": "4", "PRIME-PP 3 THRESHOLD": "80", "PRIME-PVCAM Version": "3.7.1", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Gain": "1-HDR", "640 Shutter-TriggerSequenceLength": "1000", "528 AO-SupportsTriggering": "0", "445 AO-TriggerInputLine": "/Dev1/PFI0", "OpticalPath-Name": "OpticalPath", "PRIME-PP 4 ENABLED": "Yes", "PRIME-PVCAM Adapter Version": "1.3.29", "COM3-Name": "COM3", "405 AO-MaxVolts": "5.0000", "520 Shutter-Block voltage": "false", "PRIME-Timing-ClearingTimeNs": "49344000.0000", "Core-Focus": "PIEZO", "528 AO-Block voltage": "false", "TE2000-Description": "Nikon TE2000 hub", "528 AO-MinVolts": "0.0000", "COM4-Parity": "None", "Summary": { "Slices": 11, "ChContrastMin": [ 0, 0, 0, 0, 0, 0 ], "PixelAspect": 1, "SlicesFirst": true, "Interval_ms": 0, "Time": "2019-12-18 21:36:42 -0500", "Prefix": "seq_4", "InitialPositionList": [ { "Label": "Pos0", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.21], "Focus": [17741.5], "XYStage": [ 44542.200000000004, 31636.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos1", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.22], "Focus": [17741.4], "XYStage": [ 43801.100000000006, 31637.9 ] }, "GridColumnIndex": 0 }, { "Label": "Pos2", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.5], "XYStage": [ 38561.3, 32040.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos3", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.185], "Focus": [17741.3], "XYStage": [ 37698.4, 32041.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos4", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.16], "Focus": [17741.5], "XYStage": [ 32375.4, 31758.9 ] }, "GridColumnIndex": 0 }, { "Label": "Pos5", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.17000000000002], "Focus": [17741.3], "XYStage": [ 31770.7, 31758.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos6", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.4], "XYStage": [ 26536.800000000003, 31068.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos7", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.205], "Focus": [17741.4], "XYStage": [ 25420.2, 31068.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos8", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.85], "Focus": [17741.3], "XYStage": [ 21059, 31508 ] }, "GridColumnIndex": 0 }, { "Label": "Pos9", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.82], "Focus": [17741.3], "XYStage": [ 19731.9, 31506.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos10", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.79], "Focus": [17741.4], "XYStage": [ 14528.5, 32007.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos11", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.83], "Focus": [17741.2], "XYStage": [ 13830.300000000001, 32009.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos12", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.79], "Focus": [17741.3], "XYStage": [ 8775.300000000001, 32017.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos13", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.815], "Focus": [17741.3], "XYStage": [ 8109.200000000001, 32008.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos14", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.805], "Focus": [17741.3], "XYStage": [ 3483.3, 31908.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos15", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.82], "Focus": [17741.2], "XYStage": [ 2686.8, 31903.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos16", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [211.48000000000002], "Focus": [17741.1], "XYStage": [ -1978.9, 31973.600000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos17", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [211.51500000000001], "Focus": [17741.1], "XYStage": [ -2957.8, 31982.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos18", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.195], "Focus": [17741.5], "XYStage": [ 47747.4, -576.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos19", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.23000000000002], "Focus": [17741.6], "XYStage": [ 47053.9, -576.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos20", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.205], "Focus": [17741.4], "XYStage": [ 41871.200000000004, -479.8 ] }, "GridColumnIndex": 0 }, { "Label": "Pos21", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.4], "XYStage": [ 40882.600000000006, -392 ] }, "GridColumnIndex": 0 }, { "Label": "Pos22", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.24], "Focus": [17741.4], "XYStage": [ 35905.6, -135.3 ] }, "GridColumnIndex": 0 }, { "Label": "Pos23", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.195], "Focus": [17741.5], "XYStage": [ 34990.9, -132.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos24", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.46], "Focus": [17741.4], "XYStage": [ 29678.100000000002, -958.1 ] }, "GridColumnIndex": 0 }, { "Label": "Pos25", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.44], "Focus": [17741.5], "XYStage": [ 28983.600000000002, -939.4000000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos26", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.475], "Focus": [17741.5], "XYStage": [ 23019.7, -122 ] }, "GridColumnIndex": 0 }, { "Label": "Pos27", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.44], "Focus": [17741.7], "XYStage": [ 22428, -116.80000000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos28", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.46], "Focus": [17741.7], "XYStage": [ 16364.900000000001, -181.10000000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos29", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.48000000000002], "Focus": [17741.5], "XYStage": [ 15712.1, -180 ] }, "GridColumnIndex": 0 }, { "Label": "Pos30", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.45000000000002], "Focus": [17741.7], "XYStage": [ 10346.1, 77.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos31", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.49], "Focus": [17741.4], "XYStage": [ 9755.5, 85.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos32", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.475], "Focus": [17741.5], "XYStage": [ 4775.5, 293.90000000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos33", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.445], "Focus": [17741.4], "XYStage": [ 3954.9, 293.90000000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos34", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.455], "Focus": [17741.4], "XYStage": [ -1324.9, 545.6 ] }, "GridColumnIndex": 0 }, { "Label": "Pos35", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.445], "Focus": [17741.3], "XYStage": [ -2097, 576.6 ] }, "GridColumnIndex": 0 } ], "z-step_um": 10, "GridRow": 0, "BitDepth": 16, "Depth": 2, "Channels": 6, "Source": "Micro-Manager", "ChColors": [ -16711681, -256, -65281, -1, -1, -16776961 ], "MetadataVersion": 10, "KeepShutterOpenChannels": true, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536, 65536 ], "CustomIntervals_ms": [], "Height": 1200, "MicroManagerVersion": "1.4.23 20180219", "GridColumn": 0, "UUID": "14a13bf7-c944-4672-b873-fcfbbb7ea13d", "PixelType": "GRAY16", "Width": 1200, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\Yan\\20191218-YC113-S7S8-miseq", "Frames": 1, "Comment": "", "UserName": "Nikon", "PixelSize_um": 1.1, "ROI": [ 0, 0, 1200, 1200 ], "Date": "2019-12-18", "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC", "P-DAPI-high" ], "KeepShutterOpenSlices": true, "IJType": 1, "ComputerName": "ZadorConfocal2", "Positions": 36, "PositionIndex": 9, "CameraTimeout": 20000, "TimeFirst": false }, "640 AO-Name": "AnalogIO", "Vincent-D3-Command": "Close" }, "FrameKey-0-3-3": { "COM4-StopBits": "1", "528 AO-MaxVolts": "5.0000", "XLIGHT Excitation Wheel-State": "1", "470 Shutter-Name": "AnalogIO", "Core-Shutter": "640", "555 AO-Demo": "No", "XLIGHT Dichroic-State": "2", "XYStage-StepSize-Y": "0.1000", "ZPositionUm": 228.82, "XYStage-StepSize-X": "0.1000", "PRIME-FirmwareVersion": "20.28", "PRIME-ClearCycles": "2", "520-Name": "DA Shutter", "405 Shutter-Name": "AnalogIO", "PIEZO-StepSize": "0.0050", "PRIME-ClearMode": "Pre-Exposure", "470 AO-MinVolts": "0.0000", "405 AO-SupportsTriggering": "1", "CameraChannelIndex": 0, "XYStage-StartSpeed": "500.0000", "COM9-StopBits": "2", "405 AO-Description": "NI DAC", "COM4-DelayBetweenCharsMs": "0.0000", "445 AO-Block voltage": "false", "520 AO-TriggerInputLine": "/Dev1/PFI0", "555 AO-Description": "NI DAC", "520 AO-TriggerSampleRate": "100000", "COM4-Verbose": "1", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM4-Name": "COM4", "405 Shutter-Volts": "0.0000", "Vincent-D3-Port": "COM1", "PRIME-PP 4 MIN ADU AFFECTED": "200", "470 Shutter-MinVolts": "0.0000", "PRIME-PP 3 ENABLED": "Yes", "470 AO-Description": "NI DAC", "PRIME-Description": "PVCAM API device adapter", "520 Shutter-MaxVolts": "5.0000", "520 Shutter-Volts": "0.0000", "528 AO-Sequenceable": "1", "PRIME-Binning": "1x1", "555 Shutter-MaxVolts": "5.0000", "528 Shutter-TriggerSequenceLength": "1000", "405 Shutter-Block voltage": "false", "XLIGHT Spin motor-State": "1", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "Vincent-D3-Time to open (ms)": "35.0000", "Vincent-D3-Time to close (ms)": "35.0000", "445 AO-TriggerSampleRate": "100000", "640 Shutter-IOChannel": "Dev1/ao14", "555 AO-Sequenceable": "1", "520 AO-Name": "AnalogIO", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "470 AO-Block voltage": "false", "COM1-Verbose": "1", "COM9-AnswerTimeout": "10000.0000", "528 Shutter-Name": "AnalogIO", "PRIME-Trigger Timeout (secs)": "10", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "NextFrame": 0, "470 AO-TriggerSequenceLength": "1000", "PRIME-TransposeXY": "0", "445 AO-TriggerSequenceLength": "1000", "COM1-Name": "COM1", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "COM9-DataBits": "8", "COM1-AnswerTimeout": "500.0000", "Camera": "", "TE2000-Version": "V2.50-V2.50-V2.10", "528 Shutter-Volts": "0.0000", "XLIGHT Hub-Name": "XLIGHT Hub", "PIEZO-Description": "Ludl stage driver adapter", "PositionName": "Pos9", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSampleRate": "100000", "PRIME-FanSpeedSetpoint": "High", "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "405 AO-TriggerSampleRate": "100000", "470 Shutter-Block voltage": "false", "470-State": "0", "COM3-Parity": "None", "405 AO-Volts": "0.0000", "PRIME-TransposeMirrorY": "0", "PRIME-ChipName": "GS144BSI", "470 AO-Volts": "0.0000", "PRIME-TransposeMirrorX": "0", "445 Shutter-SupportsTriggering": "0", "470 Shutter-SupportsTriggering": "0", "XLIGHT Hub-Description": "XLIGHT hub", "PRIME-PreampOffLimit": "10000", "470 AO-Sequenceable": "1", "528 Shutter-MaxVolts": "5.0000", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "Core-XYStage": "XYStage", "470 AO-TriggerSampleRate": "100000", "PRIME-Timing-PreTriggerDelayNs": "24672000.0000", "528-DA Device": "528 Shutter", "Focus-Description": "Nikon TE2000 focus adapter", "FilterBlock-State": "5", "520 AO-Demo": "No", "COM3-DelayBetweenCharsMs": "0.0000", "470 Shutter-Volts": "0.0000", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "PRIME-PP 2 THRESHOLD": "125", "PRIME-PP 4 THRESHOLD": "75", "XYStage-Acceleration": "4.0000", "470 Shutter-Demo": "No", "PRIME-Name": "PMPCIECam00", "640-DA Device": "640 Shutter", "405 AO-Block voltage": "false", "ElapsedTime-ms": 396103, "PRIME-Offset": "100", "445 AO-IOChannel": "Dev1/ao1", "640 AO-MaxVolts": "5.0000", "PositionIndex": 9, "PRIME-PP 1 ENABLED": "Yes", "COM1-BaudRate": "9600", "640-State": "1", "COM3-Verbose": "1", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "LudlController-TransmissionDelay": "0", "PRIME-FullWellCapacity": "62000", "PRIME-SMARTStreamingEnabled": "No", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "640 Shutter-Description": "NI DAC", "528-Description": "DA device that is used as a shutter", "COM4-Description": "Serial port driver (boost:asio)", "405 Shutter-Description": "NI DAC", "TE2000-Name": "TE2000", "528 Shutter-Description": "NI DAC", "COM9-Description": "Serial port driver (boost:asio)", "LudlController-Name": "LudlController", "445 Shutter-TriggerSequenceLength": "1000", "PRIME-SerialNumber": "A18B203008", "445 AO-Volts": "0.0000", "PRIME-X-dimension": "1200", "PRIME-ShutterCloseDelay": "0", "XLIGHT Disk slider-State": "2", "Position": "Default", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 AO-IOChannel": "Dev1/ao5", "520 AO-IOChannel": "Dev1/ao4", "528 AO-TriggerSequenceLength": "1000", "405 Shutter-MaxVolts": "5.0000", "470-DA Device": "470 Shutter", "PRIME-FTCapable": "No", "Core-ChannelGroup": "Channels", "555 AO-SupportsTriggering": "0", "PRIME-Y-dimension": "1200", "Frame": 0, "555 Shutter-Sequenceable": "1", "PRIME-Timing-ExposureTimeNs": "499998000.0000", "Core-ImageProcessor": "", "528-State": "0", "520 AO-Block voltage": "false", "XLIGHT Excitation Wheel-Label": "QUAD", "640 Shutter-Demo": "No", "COM3-StopBits": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "520 Shutter-IOChannel": "Dev1/ao11", "Width": 1200, "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-SupportsTriggering": "0", "640 AO-TriggerSampleRate": "100000", "520-Description": "DA device that is used as a shutter", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "405 Shutter-Sequenceable": "1", "405 Shutter-SupportsTriggering": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-TriggerSampleRate": "100000", "640 Shutter-MaxVolts": "5.0000", "555 AO-MaxVolts": "5.0000", "520 AO-TriggerSequenceLength": "1000", "COM3-BaudRate": "9600", "COM9-BaudRate": "9600", "Vincent-D3-Channel #": "Ch. #1", "PRIME-PMode": "Normal", "OpticalPath-Label": "Left(100)", "BitDepth": 16, "555 AO-TriggerSequenceLength": "1000", "445 Shutter-Description": "NI DAC", "405 AO-Sequenceable": "1", "PRIME-AcquisitionMethod": "Callbacks", "555 AO-Name": "AnalogIO", "PRIME-ShutterMode": "Pre-Exposure", "COM3-Handshaking": "Off", "445 AO-SupportsTriggering": "0", "ROI": "0-0-1200-1200", "COM9-Verbose": "1", "PRIME-Color": "OFF", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "PRIME-ShutterOpenDelay": "0", "COM1-Parity": "None", "Slice": 3, "SlicePosition": -20, "COM9-DelayBetweenCharsMs": "0.0000", "555-Name": "DA Shutter", "PIEZO-LudlSingleAxisName": "B", "XLIGHT Emission Wheel-Label": "725/40 (C)", "640 Shutter-Name": "AnalogIO", "640 AO-Sequenceable": "1", "520 Shutter-Demo": "No", "528 Shutter-Demo": "No", "470-Name": "DA Shutter", "520-DA Device": "520 Shutter", "XYStage-Description": "Ludl XY stage driver adapter", "445 Shutter-MinVolts": "0.0000", "528 AO-TriggerSampleRate": "100000", "405 AO-MinVolts": "0.0000", "405 Shutter-TriggerSequenceLength": "1000", "Height": 1200, "555 AO-Block voltage": "false", "640 Shutter-Volts": "5.0000", "PixelType": "GRAY16", "445 Shutter-Block voltage": "false", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "520 Shutter-TriggerSequenceLength": "1000", "COM9-Name": "COM9", "Core-SLM": "", "555 AO-Volts": "0.0000", "555 Shutter-Block voltage": "false", "XYStage-Speed": "1800.0000", "520 AO-MaxVolts": "5.0000", "528 Shutter-SupportsTriggering": "0", "COM1-Description": "Serial port driver (boost:asio)", "PRIME-MetadataEnabled": "No", "520 Shutter-MinVolts": "0.0000", "470 Shutter-Sequenceable": "1", "PRIME-PreampDelay": "0", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-MinVolts": "0.0000", "ChannelIndex": 3, "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Core-AutoShutter": "1", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "Exposure-ms": 520, "PRIME-PP 2 ENABLED": "Yes", "640 Shutter-TriggerSampleRate": "100000", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "470 Shutter-IOChannel": "Dev1/ao10", "405 AO-Demo": "No", "PixelSizeUm": 1.1, "470 AO-Demo": "No", "470 Shutter-MaxVolts": "5.0000", "Vincent-D3-Name": "Vincent-D3", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FileName": "img_000000000_Il-C_003.tif", "LudlController-Reset": "Operate", "PRIME-Exposure": "520.00", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "445-Name": "DA Shutter", "555 Shutter-Name": "AnalogIO", "520 Shutter-TriggerSampleRate": "100000", "445 Shutter-IOChannel": "Dev1/ao9", "PRIME-TransposeCorrection": "0", "XYStage-StepSize": "0.1000", "445-Description": "DA device that is used as a shutter", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 AO-IOChannel": "Dev1/ao7", "520 Shutter-Name": "AnalogIO", "COM3-DataBits": "8", "640 AO-Volts": "4.5000", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "555 Shutter-IOChannel": "Dev1/ao13", "WaitInterval": null, "520 Shutter-Sequenceable": "1", "YPositionUm": 31506.2, "445-State": "0", "445-DA Device": "445 Shutter", "PRIME-CircularBufferAutoSize": "ON", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "555 AO-TriggerSampleRate": "100000", "445 Shutter-Volts": "0.0000", "XLIGHT Hub-Port": "COM3", "Core-AutoFocus": "", "COM4-Handshaking": "Off", "528 Shutter-MinVolts": "0.0000", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "445 Shutter-Name": "AnalogIO", "528 Shutter-Sequenceable": "1", "XYStage-TransposeMirrorX": "0", "XYStage-TransposeMirrorY": "0", "PRIME-ReadoutRate": "100MHz 16bit", "XYStage-Name": "Stage", "640 AO-SupportsTriggering": "0", "640-Name": "DA Shutter", "640 Shutter-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "640 AO-Block voltage": "false", "445 Shutter-Sequenceable": "1", "470 AO-MaxVolts": "5.0000", "COM4-BaudRate": "9600", "470-Description": "DA device that is used as a shutter", "520 Shutter-Description": "NI DAC", "PRIME-CCDTemperature": "-14.8500", "Time": "2019-12-18 21:37:04 -0500", "640 AO-TriggerSequenceLength": "1000", "470 AO-Name": "AnalogIO", "XLIGHT Disk slider-Label": "Disk Pos 40um", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "Binning": "1x1", "TE2000-ModelType": "1", "COM3-AnswerTimeout": "500.0000", "555 Shutter-SupportsTriggering": "0", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "TE2000-Port": "COM4", "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Description": "NI DAC", "COM4-AnswerTimeout": "500.0000", "COM1-DelayBetweenCharsMs": "0.0000", "FilterBlock-Label": "Confocal", "445 AO-Name": "AnalogIO", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-Timing-ReadoutTimeNs": "24672000.0000", "528 AO-Demo": "No", "520 AO-Sequenceable": "1", "555-Description": "DA device that is used as a shutter", "405-DA Device": "405 Shutter", "405 AO-TriggerSequenceLength": "1000", "640 AO-MinVolts": "0.0000", "640 Shutter-Block voltage": "false", "FrameIndex": 0, "PRIME-Port": "CMOS", "555 AO-MinVolts": "0.0000", "COM3-Description": "Serial port driver (boost:asio)", "528 AO-Description": "NI DAC", "555 AO-IOChannel": "Dev1/ao6", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "555 AO-TriggerInputLine": "/Dev1/PFI0", "PRIME-ActualInterval-ms": "390.5200", "LudlController-Port": "COM9", "445 Shutter-Demo": "No", "PRIME-Trigger-Expose Out-Mux": "1", "SliceIndex": 3, "PRIME-CCDTemperatureSetPoint": "-15.0000", "405 Shutter-MinVolts": "0.0000", "PRIME-ExposeOutMode": "First Row", "AxisPositions": null, "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Volts": "0.0000", "528-Name": "DA Shutter", "555-State": "0", "XLIGHT Dichroic-Label": "Di03-R635-t1", "445 AO-Sequenceable": "1", "405-Name": "DA Shutter", "405 AO-IOChannel": "Dev1/ao0", "Core-Camera": "PRIME", "Focus-Name": "Focus", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "405 Shutter-IOChannel": "Dev1/ao8", "640 AO-Demo": "No", "640 Shutter-SupportsTriggering": "0", "445 AO-Demo": "No", "555-DA Device": "555 Shutter", "470 Shutter-Description": "NI DAC", "528 AO-Volts": "0.0000", "COM1-DataBits": "8", "Vincent-D3-Address": "x", "520 AO-SupportsTriggering": "0", "405-Description": "DA device that is used as a shutter", "640 Shutter-MinVolts": "0.0000", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "405 AO-Name": "AnalogIO", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "640 AO-TriggerInputLine": "/Dev1/PFI0", "405 AO-TriggerInputLine": "/Dev1/PFI0", "640-Description": "DA device that is used as a shutter", "445 Shutter-TriggerSampleRate": "100000", "470 Shutter-TriggerSequenceLength": "1000", "COM1-StopBits": "1", "520 Shutter-SupportsTriggering": "0", "405-State": "0", "Core-Initialize": "1", "XLIGHT Emission Wheel-State": "3", "520 AO-Description": "NI DAC", "528 AO-TriggerInputLine": "/Dev1/PFI0", "445 Shutter-MaxVolts": "5.0000", "COM9-Handshaking": "Off", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "XLIGHT Spin motor-Label": "On (spinning)", "PRIME-PP 5 ENABLED": "No", "UUID": "c6086b83-ae8e-4074-9fef-f6563a6d4465", "PRIME-TriggerMode": "Internal Trigger", "COM1-Handshaking": "Off", "PRIME-Timing-PostTriggerDelayNs": "49344000.0000", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "Focus-Position": "17741.5000", "528 Shutter-Block voltage": "false", "Channel": "Il-C", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "555 Shutter-Demo": "No", "405 Shutter-Demo": "No", "528 AO-Name": "AnalogIO", "520-State": "0", "Core-Galvo": "", "405 Shutter-TriggerSampleRate": "100000", "Focus-FocusStepSize_(nm)": "100", "PRIME-PixelType": "16bit", "COM4-DataBits": "8", "470 AO-IOChannel": "Dev1/ao2", "520 AO-MinVolts": "0.0000", "COM9-Parity": "None", "PRIME-CircularBufferEnabled": "ON", "PRIME-PP 6 Reset": "No", "640 AO-Description": "NI DAC", "Source": "PRIME", "445 AO-MinVolts": "0.0000", "PIEZO-Name": "Stage", "445 AO-Description": "NI DAC", "PRIME-CircularBufferFrameCount": "46", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "520 AO-Volts": "0.0000", "528 Shutter-TriggerSampleRate": "100000", "XPositionUm": 19731.9, "OpticalPath-State": "4", "PRIME-PP 3 THRESHOLD": "80", "PRIME-PVCAM Version": "3.7.1", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Gain": "1-HDR", "640 Shutter-TriggerSequenceLength": "1000", "528 AO-SupportsTriggering": "0", "445 AO-TriggerInputLine": "/Dev1/PFI0", "OpticalPath-Name": "OpticalPath", "PRIME-PP 4 ENABLED": "Yes", "PRIME-PVCAM Adapter Version": "1.3.29", "COM3-Name": "COM3", "405 AO-MaxVolts": "5.0000", "520 Shutter-Block voltage": "false", "PRIME-Timing-ClearingTimeNs": "49344000.0000", "Core-Focus": "PIEZO", "528 AO-Block voltage": "false", "TE2000-Description": "Nikon TE2000 hub", "528 AO-MinVolts": "0.0000", "COM4-Parity": "None", "Summary": { "Slices": 11, "ChContrastMin": [ 0, 0, 0, 0, 0, 0 ], "PixelAspect": 1, "SlicesFirst": true, "Interval_ms": 0, "Time": "2019-12-18 21:36:42 -0500", "Prefix": "seq_4", "InitialPositionList": [ { "Label": "Pos0", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.21], "Focus": [17741.5], "XYStage": [ 44542.200000000004, 31636.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos1", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.22], "Focus": [17741.4], "XYStage": [ 43801.100000000006, 31637.9 ] }, "GridColumnIndex": 0 }, { "Label": "Pos2", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.5], "XYStage": [ 38561.3, 32040.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos3", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.185], "Focus": [17741.3], "XYStage": [ 37698.4, 32041.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos4", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.16], "Focus": [17741.5], "XYStage": [ 32375.4, 31758.9 ] }, "GridColumnIndex": 0 }, { "Label": "Pos5", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.17000000000002], "Focus": [17741.3], "XYStage": [ 31770.7, 31758.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos6", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.4], "XYStage": [ 26536.800000000003, 31068.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos7", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.205], "Focus": [17741.4], "XYStage": [ 25420.2, 31068.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos8", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.85], "Focus": [17741.3], "XYStage": [ 21059, 31508 ] }, "GridColumnIndex": 0 }, { "Label": "Pos9", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.82], "Focus": [17741.3], "XYStage": [ 19731.9, 31506.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos10", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.79], "Focus": [17741.4], "XYStage": [ 14528.5, 32007.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos11", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.83], "Focus": [17741.2], "XYStage": [ 13830.300000000001, 32009.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos12", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.79], "Focus": [17741.3], "XYStage": [ 8775.300000000001, 32017.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos13", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.815], "Focus": [17741.3], "XYStage": [ 8109.200000000001, 32008.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos14", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.805], "Focus": [17741.3], "XYStage": [ 3483.3, 31908.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos15", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.82], "Focus": [17741.2], "XYStage": [ 2686.8, 31903.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos16", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [211.48000000000002], "Focus": [17741.1], "XYStage": [ -1978.9, 31973.600000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos17", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [211.51500000000001], "Focus": [17741.1], "XYStage": [ -2957.8, 31982.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos18", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.195], "Focus": [17741.5], "XYStage": [ 47747.4, -576.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos19", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.23000000000002], "Focus": [17741.6], "XYStage": [ 47053.9, -576.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos20", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.205], "Focus": [17741.4], "XYStage": [ 41871.200000000004, -479.8 ] }, "GridColumnIndex": 0 }, { "Label": "Pos21", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.4], "XYStage": [ 40882.600000000006, -392 ] }, "GridColumnIndex": 0 }, { "Label": "Pos22", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.24], "Focus": [17741.4], "XYStage": [ 35905.6, -135.3 ] }, "GridColumnIndex": 0 }, { "Label": "Pos23", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.195], "Focus": [17741.5], "XYStage": [ 34990.9, -132.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos24", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.46], "Focus": [17741.4], "XYStage": [ 29678.100000000002, -958.1 ] }, "GridColumnIndex": 0 }, { "Label": "Pos25", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.44], "Focus": [17741.5], "XYStage": [ 28983.600000000002, -939.4000000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos26", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.475], "Focus": [17741.5], "XYStage": [ 23019.7, -122 ] }, "GridColumnIndex": 0 }, { "Label": "Pos27", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.44], "Focus": [17741.7], "XYStage": [ 22428, -116.80000000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos28", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.46], "Focus": [17741.7], "XYStage": [ 16364.900000000001, -181.10000000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos29", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.48000000000002], "Focus": [17741.5], "XYStage": [ 15712.1, -180 ] }, "GridColumnIndex": 0 }, { "Label": "Pos30", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.45000000000002], "Focus": [17741.7], "XYStage": [ 10346.1, 77.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos31", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.49], "Focus": [17741.4], "XYStage": [ 9755.5, 85.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos32", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.475], "Focus": [17741.5], "XYStage": [ 4775.5, 293.90000000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos33", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.445], "Focus": [17741.4], "XYStage": [ 3954.9, 293.90000000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos34", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.455], "Focus": [17741.4], "XYStage": [ -1324.9, 545.6 ] }, "GridColumnIndex": 0 }, { "Label": "Pos35", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.445], "Focus": [17741.3], "XYStage": [ -2097, 576.6 ] }, "GridColumnIndex": 0 } ], "z-step_um": 10, "GridRow": 0, "BitDepth": 16, "Depth": 2, "Channels": 6, "Source": "Micro-Manager", "ChColors": [ -16711681, -256, -65281, -1, -1, -16776961 ], "MetadataVersion": 10, "KeepShutterOpenChannels": true, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536, 65536 ], "CustomIntervals_ms": [], "Height": 1200, "MicroManagerVersion": "1.4.23 20180219", "GridColumn": 0, "UUID": "14a13bf7-c944-4672-b873-fcfbbb7ea13d", "PixelType": "GRAY16", "Width": 1200, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\Yan\\20191218-YC113-S7S8-miseq", "Frames": 1, "Comment": "", "UserName": "Nikon", "PixelSize_um": 1.1, "ROI": [ 0, 0, 1200, 1200 ], "Date": "2019-12-18", "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC", "P-DAPI-high" ], "KeepShutterOpenSlices": true, "IJType": 1, "ComputerName": "ZadorConfocal2", "Positions": 36, "PositionIndex": 9, "CameraTimeout": 20000, "TimeFirst": false }, "640 AO-Name": "AnalogIO", "Vincent-D3-Command": "Close" }, "FrameKey-0-3-4": { "COM4-StopBits": "1", "528 AO-MaxVolts": "5.0000", "XLIGHT Excitation Wheel-State": "1", "470 Shutter-Name": "AnalogIO", "Core-Shutter": "640", "555 AO-Demo": "No", "XLIGHT Dichroic-State": "2", "XYStage-StepSize-Y": "0.1000", "ZPositionUm": 238.82, "XYStage-StepSize-X": "0.1000", "PRIME-FirmwareVersion": "20.28", "PRIME-ClearCycles": "2", "520-Name": "DA Shutter", "405 Shutter-Name": "AnalogIO", "PIEZO-StepSize": "0.0050", "PRIME-ClearMode": "Pre-Exposure", "470 AO-MinVolts": "0.0000", "405 AO-SupportsTriggering": "1", "CameraChannelIndex": 0, "XYStage-StartSpeed": "500.0000", "COM9-StopBits": "2", "405 AO-Description": "NI DAC", "COM4-DelayBetweenCharsMs": "0.0000", "445 AO-Block voltage": "false", "520 AO-TriggerInputLine": "/Dev1/PFI0", "555 AO-Description": "NI DAC", "520 AO-TriggerSampleRate": "100000", "COM4-Verbose": "1", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM4-Name": "COM4", "405 Shutter-Volts": "0.0000", "Vincent-D3-Port": "COM1", "PRIME-PP 4 MIN ADU AFFECTED": "200", "470 Shutter-MinVolts": "0.0000", "PRIME-PP 3 ENABLED": "Yes", "470 AO-Description": "NI DAC", "PRIME-Description": "PVCAM API device adapter", "520 Shutter-MaxVolts": "5.0000", "520 Shutter-Volts": "0.0000", "528 AO-Sequenceable": "1", "PRIME-Binning": "1x1", "555 Shutter-MaxVolts": "5.0000", "528 Shutter-TriggerSequenceLength": "1000", "405 Shutter-Block voltage": "false", "XLIGHT Spin motor-State": "1", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "Vincent-D3-Time to open (ms)": "35.0000", "Vincent-D3-Time to close (ms)": "35.0000", "445 AO-TriggerSampleRate": "100000", "640 Shutter-IOChannel": "Dev1/ao14", "555 AO-Sequenceable": "1", "520 AO-Name": "AnalogIO", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "470 AO-Block voltage": "false", "COM1-Verbose": "1", "COM9-AnswerTimeout": "10000.0000", "528 Shutter-Name": "AnalogIO", "PRIME-Trigger Timeout (secs)": "10", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "NextFrame": 0, "470 AO-TriggerSequenceLength": "1000", "PRIME-TransposeXY": "0", "445 AO-TriggerSequenceLength": "1000", "COM1-Name": "COM1", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "COM9-DataBits": "8", "COM1-AnswerTimeout": "500.0000", "Camera": "", "TE2000-Version": "V2.50-V2.50-V2.10", "528 Shutter-Volts": "0.0000", "XLIGHT Hub-Name": "XLIGHT Hub", "PIEZO-Description": "Ludl stage driver adapter", "PositionName": "Pos9", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSampleRate": "100000", "PRIME-FanSpeedSetpoint": "High", "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "405 AO-TriggerSampleRate": "100000", "470 Shutter-Block voltage": "false", "470-State": "0", "COM3-Parity": "None", "405 AO-Volts": "0.0000", "PRIME-TransposeMirrorY": "0", "PRIME-ChipName": "GS144BSI", "470 AO-Volts": "0.0000", "PRIME-TransposeMirrorX": "0", "445 Shutter-SupportsTriggering": "0", "470 Shutter-SupportsTriggering": "0", "XLIGHT Hub-Description": "XLIGHT hub", "PRIME-PreampOffLimit": "10000", "470 AO-Sequenceable": "1", "528 Shutter-MaxVolts": "5.0000", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "Core-XYStage": "XYStage", "470 AO-TriggerSampleRate": "100000", "PRIME-Timing-PreTriggerDelayNs": "24672000.0000", "528-DA Device": "528 Shutter", "Focus-Description": "Nikon TE2000 focus adapter", "FilterBlock-State": "5", "520 AO-Demo": "No", "COM3-DelayBetweenCharsMs": "0.0000", "470 Shutter-Volts": "0.0000", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "PRIME-PP 2 THRESHOLD": "125", "PRIME-PP 4 THRESHOLD": "75", "XYStage-Acceleration": "4.0000", "470 Shutter-Demo": "No", "PRIME-Name": "PMPCIECam00", "640-DA Device": "640 Shutter", "405 AO-Block voltage": "false", "ElapsedTime-ms": 396865, "PRIME-Offset": "100", "445 AO-IOChannel": "Dev1/ao1", "640 AO-MaxVolts": "5.0000", "PositionIndex": 9, "PRIME-PP 1 ENABLED": "Yes", "COM1-BaudRate": "9600", "640-State": "1", "COM3-Verbose": "1", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "LudlController-TransmissionDelay": "0", "PRIME-FullWellCapacity": "62000", "PRIME-SMARTStreamingEnabled": "No", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "640 Shutter-Description": "NI DAC", "528-Description": "DA device that is used as a shutter", "COM4-Description": "Serial port driver (boost:asio)", "405 Shutter-Description": "NI DAC", "TE2000-Name": "TE2000", "528 Shutter-Description": "NI DAC", "COM9-Description": "Serial port driver (boost:asio)", "LudlController-Name": "LudlController", "445 Shutter-TriggerSequenceLength": "1000", "PRIME-SerialNumber": "A18B203008", "445 AO-Volts": "0.0000", "PRIME-X-dimension": "1200", "PRIME-ShutterCloseDelay": "0", "XLIGHT Disk slider-State": "2", "Position": "Default", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 AO-IOChannel": "Dev1/ao5", "520 AO-IOChannel": "Dev1/ao4", "528 AO-TriggerSequenceLength": "1000", "405 Shutter-MaxVolts": "5.0000", "470-DA Device": "470 Shutter", "PRIME-FTCapable": "No", "Core-ChannelGroup": "Channels", "555 AO-SupportsTriggering": "0", "PRIME-Y-dimension": "1200", "Frame": 0, "555 Shutter-Sequenceable": "1", "PRIME-Timing-ExposureTimeNs": "499998000.0000", "Core-ImageProcessor": "", "528-State": "0", "520 AO-Block voltage": "false", "XLIGHT Excitation Wheel-Label": "QUAD", "640 Shutter-Demo": "No", "COM3-StopBits": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "520 Shutter-IOChannel": "Dev1/ao11", "Width": 1200, "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-SupportsTriggering": "0", "640 AO-TriggerSampleRate": "100000", "520-Description": "DA device that is used as a shutter", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "405 Shutter-Sequenceable": "1", "405 Shutter-SupportsTriggering": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-TriggerSampleRate": "100000", "640 Shutter-MaxVolts": "5.0000", "555 AO-MaxVolts": "5.0000", "520 AO-TriggerSequenceLength": "1000", "COM3-BaudRate": "9600", "COM9-BaudRate": "9600", "Vincent-D3-Channel #": "Ch. #1", "PRIME-PMode": "Normal", "OpticalPath-Label": "Left(100)", "BitDepth": 16, "555 AO-TriggerSequenceLength": "1000", "445 Shutter-Description": "NI DAC", "405 AO-Sequenceable": "1", "PRIME-AcquisitionMethod": "Callbacks", "555 AO-Name": "AnalogIO", "PRIME-ShutterMode": "Pre-Exposure", "COM3-Handshaking": "Off", "445 AO-SupportsTriggering": "0", "ROI": "0-0-1200-1200", "COM9-Verbose": "1", "PRIME-Color": "OFF", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "PRIME-ShutterOpenDelay": "0", "COM1-Parity": "None", "Slice": 4, "SlicePosition": -10, "COM9-DelayBetweenCharsMs": "0.0000", "555-Name": "DA Shutter", "PIEZO-LudlSingleAxisName": "B", "XLIGHT Emission Wheel-Label": "725/40 (C)", "640 Shutter-Name": "AnalogIO", "640 AO-Sequenceable": "1", "520 Shutter-Demo": "No", "528 Shutter-Demo": "No", "470-Name": "DA Shutter", "520-DA Device": "520 Shutter", "XYStage-Description": "Ludl XY stage driver adapter", "445 Shutter-MinVolts": "0.0000", "528 AO-TriggerSampleRate": "100000", "405 AO-MinVolts": "0.0000", "405 Shutter-TriggerSequenceLength": "1000", "Height": 1200, "555 AO-Block voltage": "false", "640 Shutter-Volts": "5.0000", "PixelType": "GRAY16", "445 Shutter-Block voltage": "false", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "520 Shutter-TriggerSequenceLength": "1000", "COM9-Name": "COM9", "Core-SLM": "", "555 AO-Volts": "0.0000", "555 Shutter-Block voltage": "false", "XYStage-Speed": "1800.0000", "520 AO-MaxVolts": "5.0000", "528 Shutter-SupportsTriggering": "0", "COM1-Description": "Serial port driver (boost:asio)", "PRIME-MetadataEnabled": "No", "520 Shutter-MinVolts": "0.0000", "470 Shutter-Sequenceable": "1", "PRIME-PreampDelay": "0", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-MinVolts": "0.0000", "ChannelIndex": 3, "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Core-AutoShutter": "1", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "Exposure-ms": 520, "PRIME-PP 2 ENABLED": "Yes", "640 Shutter-TriggerSampleRate": "100000", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "470 Shutter-IOChannel": "Dev1/ao10", "405 AO-Demo": "No", "PixelSizeUm": 1.1, "470 AO-Demo": "No", "470 Shutter-MaxVolts": "5.0000", "Vincent-D3-Name": "Vincent-D3", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FileName": "img_000000000_Il-C_004.tif", "LudlController-Reset": "Operate", "PRIME-Exposure": "520.00", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "445-Name": "DA Shutter", "555 Shutter-Name": "AnalogIO", "520 Shutter-TriggerSampleRate": "100000", "445 Shutter-IOChannel": "Dev1/ao9", "PRIME-TransposeCorrection": "0", "XYStage-StepSize": "0.1000", "445-Description": "DA device that is used as a shutter", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 AO-IOChannel": "Dev1/ao7", "520 Shutter-Name": "AnalogIO", "COM3-DataBits": "8", "640 AO-Volts": "4.5000", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "555 Shutter-IOChannel": "Dev1/ao13", "WaitInterval": null, "520 Shutter-Sequenceable": "1", "YPositionUm": 31506.2, "445-State": "0", "445-DA Device": "445 Shutter", "PRIME-CircularBufferAutoSize": "ON", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "555 AO-TriggerSampleRate": "100000", "445 Shutter-Volts": "0.0000", "XLIGHT Hub-Port": "COM3", "Core-AutoFocus": "", "COM4-Handshaking": "Off", "528 Shutter-MinVolts": "0.0000", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "445 Shutter-Name": "AnalogIO", "528 Shutter-Sequenceable": "1", "XYStage-TransposeMirrorX": "0", "XYStage-TransposeMirrorY": "0", "PRIME-ReadoutRate": "100MHz 16bit", "XYStage-Name": "Stage", "640 AO-SupportsTriggering": "0", "640-Name": "DA Shutter", "640 Shutter-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "640 AO-Block voltage": "false", "445 Shutter-Sequenceable": "1", "470 AO-MaxVolts": "5.0000", "COM4-BaudRate": "9600", "470-Description": "DA device that is used as a shutter", "520 Shutter-Description": "NI DAC", "PRIME-CCDTemperature": "-14.8500", "Time": "2019-12-18 21:37:04 -0500", "640 AO-TriggerSequenceLength": "1000", "470 AO-Name": "AnalogIO", "XLIGHT Disk slider-Label": "Disk Pos 40um", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "Binning": "1x1", "TE2000-ModelType": "1", "COM3-AnswerTimeout": "500.0000", "555 Shutter-SupportsTriggering": "0", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "TE2000-Port": "COM4", "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Description": "NI DAC", "COM4-AnswerTimeout": "500.0000", "COM1-DelayBetweenCharsMs": "0.0000", "FilterBlock-Label": "Confocal", "445 AO-Name": "AnalogIO", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-Timing-ReadoutTimeNs": "24672000.0000", "528 AO-Demo": "No", "520 AO-Sequenceable": "1", "555-Description": "DA device that is used as a shutter", "405-DA Device": "405 Shutter", "405 AO-TriggerSequenceLength": "1000", "640 AO-MinVolts": "0.0000", "640 Shutter-Block voltage": "false", "FrameIndex": 0, "PRIME-Port": "CMOS", "555 AO-MinVolts": "0.0000", "COM3-Description": "Serial port driver (boost:asio)", "528 AO-Description": "NI DAC", "555 AO-IOChannel": "Dev1/ao6", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "555 AO-TriggerInputLine": "/Dev1/PFI0", "PRIME-ActualInterval-ms": "390.5200", "LudlController-Port": "COM9", "445 Shutter-Demo": "No", "PRIME-Trigger-Expose Out-Mux": "1", "SliceIndex": 4, "PRIME-CCDTemperatureSetPoint": "-15.0000", "405 Shutter-MinVolts": "0.0000", "PRIME-ExposeOutMode": "First Row", "AxisPositions": null, "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Volts": "0.0000", "528-Name": "DA Shutter", "555-State": "0", "XLIGHT Dichroic-Label": "Di03-R635-t1", "445 AO-Sequenceable": "1", "405-Name": "DA Shutter", "405 AO-IOChannel": "Dev1/ao0", "Core-Camera": "PRIME", "Focus-Name": "Focus", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "405 Shutter-IOChannel": "Dev1/ao8", "640 AO-Demo": "No", "640 Shutter-SupportsTriggering": "0", "445 AO-Demo": "No", "555-DA Device": "555 Shutter", "470 Shutter-Description": "NI DAC", "528 AO-Volts": "0.0000", "COM1-DataBits": "8", "Vincent-D3-Address": "x", "520 AO-SupportsTriggering": "0", "405-Description": "DA device that is used as a shutter", "640 Shutter-MinVolts": "0.0000", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "405 AO-Name": "AnalogIO", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "640 AO-TriggerInputLine": "/Dev1/PFI0", "405 AO-TriggerInputLine": "/Dev1/PFI0", "640-Description": "DA device that is used as a shutter", "445 Shutter-TriggerSampleRate": "100000", "470 Shutter-TriggerSequenceLength": "1000", "COM1-StopBits": "1", "520 Shutter-SupportsTriggering": "0", "405-State": "0", "Core-Initialize": "1", "XLIGHT Emission Wheel-State": "3", "520 AO-Description": "NI DAC", "528 AO-TriggerInputLine": "/Dev1/PFI0", "445 Shutter-MaxVolts": "5.0000", "COM9-Handshaking": "Off", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "XLIGHT Spin motor-Label": "On (spinning)", "PRIME-PP 5 ENABLED": "No", "UUID": "d70c46c0-7943-4567-9d36-21e8098d65db", "PRIME-TriggerMode": "Internal Trigger", "COM1-Handshaking": "Off", "PRIME-Timing-PostTriggerDelayNs": "49344000.0000", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "Focus-Position": "17741.5000", "528 Shutter-Block voltage": "false", "Channel": "Il-C", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "555 Shutter-Demo": "No", "405 Shutter-Demo": "No", "528 AO-Name": "AnalogIO", "520-State": "0", "Core-Galvo": "", "405 Shutter-TriggerSampleRate": "100000", "Focus-FocusStepSize_(nm)": "100", "PRIME-PixelType": "16bit", "COM4-DataBits": "8", "470 AO-IOChannel": "Dev1/ao2", "520 AO-MinVolts": "0.0000", "COM9-Parity": "None", "PRIME-CircularBufferEnabled": "ON", "PRIME-PP 6 Reset": "No", "640 AO-Description": "NI DAC", "Source": "PRIME", "445 AO-MinVolts": "0.0000", "PIEZO-Name": "Stage", "445 AO-Description": "NI DAC", "PRIME-CircularBufferFrameCount": "46", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "520 AO-Volts": "0.0000", "528 Shutter-TriggerSampleRate": "100000", "XPositionUm": 19731.9, "OpticalPath-State": "4", "PRIME-PP 3 THRESHOLD": "80", "PRIME-PVCAM Version": "3.7.1", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Gain": "1-HDR", "640 Shutter-TriggerSequenceLength": "1000", "528 AO-SupportsTriggering": "0", "445 AO-TriggerInputLine": "/Dev1/PFI0", "OpticalPath-Name": "OpticalPath", "PRIME-PP 4 ENABLED": "Yes", "PRIME-PVCAM Adapter Version": "1.3.29", "COM3-Name": "COM3", "405 AO-MaxVolts": "5.0000", "520 Shutter-Block voltage": "false", "PRIME-Timing-ClearingTimeNs": "49344000.0000", "Core-Focus": "PIEZO", "528 AO-Block voltage": "false", "TE2000-Description": "Nikon TE2000 hub", "528 AO-MinVolts": "0.0000", "COM4-Parity": "None", "Summary": { "Slices": 11, "ChContrastMin": [ 0, 0, 0, 0, 0, 0 ], "PixelAspect": 1, "SlicesFirst": true, "Interval_ms": 0, "Time": "2019-12-18 21:36:42 -0500", "Prefix": "seq_4", "InitialPositionList": [ { "Label": "Pos0", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.21], "Focus": [17741.5], "XYStage": [ 44542.200000000004, 31636.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos1", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.22], "Focus": [17741.4], "XYStage": [ 43801.100000000006, 31637.9 ] }, "GridColumnIndex": 0 }, { "Label": "Pos2", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.5], "XYStage": [ 38561.3, 32040.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos3", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.185], "Focus": [17741.3], "XYStage": [ 37698.4, 32041.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos4", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.16], "Focus": [17741.5], "XYStage": [ 32375.4, 31758.9 ] }, "GridColumnIndex": 0 }, { "Label": "Pos5", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.17000000000002], "Focus": [17741.3], "XYStage": [ 31770.7, 31758.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos6", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.4], "XYStage": [ 26536.800000000003, 31068.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos7", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.205], "Focus": [17741.4], "XYStage": [ 25420.2, 31068.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos8", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.85], "Focus": [17741.3], "XYStage": [ 21059, 31508 ] }, "GridColumnIndex": 0 }, { "Label": "Pos9", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.82], "Focus": [17741.3], "XYStage": [ 19731.9, 31506.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos10", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.79], "Focus": [17741.4], "XYStage": [ 14528.5, 32007.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos11", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.83], "Focus": [17741.2], "XYStage": [ 13830.300000000001, 32009.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos12", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.79], "Focus": [17741.3], "XYStage": [ 8775.300000000001, 32017.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos13", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.815], "Focus": [17741.3], "XYStage": [ 8109.200000000001, 32008.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos14", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.805], "Focus": [17741.3], "XYStage": [ 3483.3, 31908.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos15", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.82], "Focus": [17741.2], "XYStage": [ 2686.8, 31903.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos16", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [211.48000000000002], "Focus": [17741.1], "XYStage": [ -1978.9, 31973.600000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos17", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [211.51500000000001], "Focus": [17741.1], "XYStage": [ -2957.8, 31982.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos18", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.195], "Focus": [17741.5], "XYStage": [ 47747.4, -576.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos19", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.23000000000002], "Focus": [17741.6], "XYStage": [ 47053.9, -576.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos20", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.205], "Focus": [17741.4], "XYStage": [ 41871.200000000004, -479.8 ] }, "GridColumnIndex": 0 }, { "Label": "Pos21", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.4], "XYStage": [ 40882.600000000006, -392 ] }, "GridColumnIndex": 0 }, { "Label": "Pos22", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.24], "Focus": [17741.4], "XYStage": [ 35905.6, -135.3 ] }, "GridColumnIndex": 0 }, { "Label": "Pos23", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.195], "Focus": [17741.5], "XYStage": [ 34990.9, -132.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos24", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.46], "Focus": [17741.4], "XYStage": [ 29678.100000000002, -958.1 ] }, "GridColumnIndex": 0 }, { "Label": "Pos25", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.44], "Focus": [17741.5], "XYStage": [ 28983.600000000002, -939.4000000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos26", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.475], "Focus": [17741.5], "XYStage": [ 23019.7, -122 ] }, "GridColumnIndex": 0 }, { "Label": "Pos27", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.44], "Focus": [17741.7], "XYStage": [ 22428, -116.80000000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos28", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.46], "Focus": [17741.7], "XYStage": [ 16364.900000000001, -181.10000000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos29", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.48000000000002], "Focus": [17741.5], "XYStage": [ 15712.1, -180 ] }, "GridColumnIndex": 0 }, { "Label": "Pos30", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.45000000000002], "Focus": [17741.7], "XYStage": [ 10346.1, 77.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos31", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.49], "Focus": [17741.4], "XYStage": [ 9755.5, 85.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos32", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.475], "Focus": [17741.5], "XYStage": [ 4775.5, 293.90000000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos33", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.445], "Focus": [17741.4], "XYStage": [ 3954.9, 293.90000000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos34", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.455], "Focus": [17741.4], "XYStage": [ -1324.9, 545.6 ] }, "GridColumnIndex": 0 }, { "Label": "Pos35", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.445], "Focus": [17741.3], "XYStage": [ -2097, 576.6 ] }, "GridColumnIndex": 0 } ], "z-step_um": 10, "GridRow": 0, "BitDepth": 16, "Depth": 2, "Channels": 6, "Source": "Micro-Manager", "ChColors": [ -16711681, -256, -65281, -1, -1, -16776961 ], "MetadataVersion": 10, "KeepShutterOpenChannels": true, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536, 65536 ], "CustomIntervals_ms": [], "Height": 1200, "MicroManagerVersion": "1.4.23 20180219", "GridColumn": 0, "UUID": "14a13bf7-c944-4672-b873-fcfbbb7ea13d", "PixelType": "GRAY16", "Width": 1200, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\Yan\\20191218-YC113-S7S8-miseq", "Frames": 1, "Comment": "", "UserName": "Nikon", "PixelSize_um": 1.1, "ROI": [ 0, 0, 1200, 1200 ], "Date": "2019-12-18", "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC", "P-DAPI-high" ], "KeepShutterOpenSlices": true, "IJType": 1, "ComputerName": "ZadorConfocal2", "Positions": 36, "PositionIndex": 9, "CameraTimeout": 20000, "TimeFirst": false }, "640 AO-Name": "AnalogIO", "Vincent-D3-Command": "Close" }, "FrameKey-0-3-5": { "COM4-StopBits": "1", "528 AO-MaxVolts": "5.0000", "XLIGHT Excitation Wheel-State": "1", "470 Shutter-Name": "AnalogIO", "Core-Shutter": "640", "555 AO-Demo": "No", "XLIGHT Dichroic-State": "2", "XYStage-StepSize-Y": "0.1000", "ZPositionUm": 248.82, "XYStage-StepSize-X": "0.1000", "PRIME-FirmwareVersion": "20.28", "PRIME-ClearCycles": "2", "520-Name": "DA Shutter", "405 Shutter-Name": "AnalogIO", "PIEZO-StepSize": "0.0050", "PRIME-ClearMode": "Pre-Exposure", "470 AO-MinVolts": "0.0000", "405 AO-SupportsTriggering": "1", "CameraChannelIndex": 0, "XYStage-StartSpeed": "500.0000", "COM9-StopBits": "2", "405 AO-Description": "NI DAC", "COM4-DelayBetweenCharsMs": "0.0000", "445 AO-Block voltage": "false", "520 AO-TriggerInputLine": "/Dev1/PFI0", "555 AO-Description": "NI DAC", "520 AO-TriggerSampleRate": "100000", "COM4-Verbose": "1", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM4-Name": "COM4", "405 Shutter-Volts": "0.0000", "Vincent-D3-Port": "COM1", "PRIME-PP 4 MIN ADU AFFECTED": "200", "470 Shutter-MinVolts": "0.0000", "PRIME-PP 3 ENABLED": "Yes", "470 AO-Description": "NI DAC", "PRIME-Description": "PVCAM API device adapter", "520 Shutter-MaxVolts": "5.0000", "520 Shutter-Volts": "0.0000", "528 AO-Sequenceable": "1", "PRIME-Binning": "1x1", "555 Shutter-MaxVolts": "5.0000", "528 Shutter-TriggerSequenceLength": "1000", "405 Shutter-Block voltage": "false", "XLIGHT Spin motor-State": "1", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "Vincent-D3-Time to open (ms)": "35.0000", "Vincent-D3-Time to close (ms)": "35.0000", "445 AO-TriggerSampleRate": "100000", "640 Shutter-IOChannel": "Dev1/ao14", "555 AO-Sequenceable": "1", "520 AO-Name": "AnalogIO", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "470 AO-Block voltage": "false", "COM1-Verbose": "1", "COM9-AnswerTimeout": "10000.0000", "528 Shutter-Name": "AnalogIO", "PRIME-Trigger Timeout (secs)": "10", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "NextFrame": 0, "470 AO-TriggerSequenceLength": "1000", "PRIME-TransposeXY": "0", "445 AO-TriggerSequenceLength": "1000", "COM1-Name": "COM1", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "COM9-DataBits": "8", "COM1-AnswerTimeout": "500.0000", "Camera": "", "TE2000-Version": "V2.50-V2.50-V2.10", "528 Shutter-Volts": "0.0000", "XLIGHT Hub-Name": "XLIGHT Hub", "PIEZO-Description": "Ludl stage driver adapter", "PositionName": "Pos9", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSampleRate": "100000", "PRIME-FanSpeedSetpoint": "High", "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "405 AO-TriggerSampleRate": "100000", "470 Shutter-Block voltage": "false", "470-State": "0", "COM3-Parity": "None", "405 AO-Volts": "0.0000", "PRIME-TransposeMirrorY": "0", "PRIME-ChipName": "GS144BSI", "470 AO-Volts": "0.0000", "PRIME-TransposeMirrorX": "0", "445 Shutter-SupportsTriggering": "0", "470 Shutter-SupportsTriggering": "0", "XLIGHT Hub-Description": "XLIGHT hub", "PRIME-PreampOffLimit": "10000", "470 AO-Sequenceable": "1", "528 Shutter-MaxVolts": "5.0000", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "Core-XYStage": "XYStage", "470 AO-TriggerSampleRate": "100000", "PRIME-Timing-PreTriggerDelayNs": "24672000.0000", "528-DA Device": "528 Shutter", "Focus-Description": "Nikon TE2000 focus adapter", "FilterBlock-State": "5", "520 AO-Demo": "No", "COM3-DelayBetweenCharsMs": "0.0000", "470 Shutter-Volts": "0.0000", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "PRIME-PP 2 THRESHOLD": "125", "PRIME-PP 4 THRESHOLD": "75", "XYStage-Acceleration": "4.0000", "470 Shutter-Demo": "No", "PRIME-Name": "PMPCIECam00", "640-DA Device": "640 Shutter", "405 AO-Block voltage": "false", "ElapsedTime-ms": 397685, "PRIME-Offset": "100", "445 AO-IOChannel": "Dev1/ao1", "640 AO-MaxVolts": "5.0000", "PositionIndex": 9, "PRIME-PP 1 ENABLED": "Yes", "COM1-BaudRate": "9600", "640-State": "1", "COM3-Verbose": "1", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "LudlController-TransmissionDelay": "0", "PRIME-FullWellCapacity": "62000", "PRIME-SMARTStreamingEnabled": "No", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "640 Shutter-Description": "NI DAC", "528-Description": "DA device that is used as a shutter", "COM4-Description": "Serial port driver (boost:asio)", "405 Shutter-Description": "NI DAC", "TE2000-Name": "TE2000", "528 Shutter-Description": "NI DAC", "COM9-Description": "Serial port driver (boost:asio)", "LudlController-Name": "LudlController", "445 Shutter-TriggerSequenceLength": "1000", "PRIME-SerialNumber": "A18B203008", "445 AO-Volts": "0.0000", "PRIME-X-dimension": "1200", "PRIME-ShutterCloseDelay": "0", "XLIGHT Disk slider-State": "2", "Position": "Default", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 AO-IOChannel": "Dev1/ao5", "520 AO-IOChannel": "Dev1/ao4", "528 AO-TriggerSequenceLength": "1000", "405 Shutter-MaxVolts": "5.0000", "470-DA Device": "470 Shutter", "PRIME-FTCapable": "No", "Core-ChannelGroup": "Channels", "555 AO-SupportsTriggering": "0", "PRIME-Y-dimension": "1200", "Frame": 0, "555 Shutter-Sequenceable": "1", "PRIME-Timing-ExposureTimeNs": "499998000.0000", "Core-ImageProcessor": "", "528-State": "0", "520 AO-Block voltage": "false", "XLIGHT Excitation Wheel-Label": "QUAD", "640 Shutter-Demo": "No", "COM3-StopBits": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "520 Shutter-IOChannel": "Dev1/ao11", "Width": 1200, "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-SupportsTriggering": "0", "640 AO-TriggerSampleRate": "100000", "520-Description": "DA device that is used as a shutter", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "405 Shutter-Sequenceable": "1", "405 Shutter-SupportsTriggering": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-TriggerSampleRate": "100000", "640 Shutter-MaxVolts": "5.0000", "555 AO-MaxVolts": "5.0000", "520 AO-TriggerSequenceLength": "1000", "COM3-BaudRate": "9600", "COM9-BaudRate": "9600", "Vincent-D3-Channel #": "Ch. #1", "PRIME-PMode": "Normal", "OpticalPath-Label": "Left(100)", "BitDepth": 16, "555 AO-TriggerSequenceLength": "1000", "445 Shutter-Description": "NI DAC", "405 AO-Sequenceable": "1", "PRIME-AcquisitionMethod": "Callbacks", "555 AO-Name": "AnalogIO", "PRIME-ShutterMode": "Pre-Exposure", "COM3-Handshaking": "Off", "445 AO-SupportsTriggering": "0", "ROI": "0-0-1200-1200", "COM9-Verbose": "1", "PRIME-Color": "OFF", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "PRIME-ShutterOpenDelay": "0", "COM1-Parity": "None", "Slice": 5, "SlicePosition": 0, "COM9-DelayBetweenCharsMs": "0.0000", "555-Name": "DA Shutter", "PIEZO-LudlSingleAxisName": "B", "XLIGHT Emission Wheel-Label": "725/40 (C)", "640 Shutter-Name": "AnalogIO", "640 AO-Sequenceable": "1", "520 Shutter-Demo": "No", "528 Shutter-Demo": "No", "470-Name": "DA Shutter", "520-DA Device": "520 Shutter", "XYStage-Description": "Ludl XY stage driver adapter", "445 Shutter-MinVolts": "0.0000", "528 AO-TriggerSampleRate": "100000", "405 AO-MinVolts": "0.0000", "405 Shutter-TriggerSequenceLength": "1000", "Height": 1200, "555 AO-Block voltage": "false", "640 Shutter-Volts": "5.0000", "PixelType": "GRAY16", "445 Shutter-Block voltage": "false", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "520 Shutter-TriggerSequenceLength": "1000", "COM9-Name": "COM9", "Core-SLM": "", "555 AO-Volts": "0.0000", "555 Shutter-Block voltage": "false", "XYStage-Speed": "1800.0000", "520 AO-MaxVolts": "5.0000", "528 Shutter-SupportsTriggering": "0", "COM1-Description": "Serial port driver (boost:asio)", "PRIME-MetadataEnabled": "No", "520 Shutter-MinVolts": "0.0000", "470 Shutter-Sequenceable": "1", "PRIME-PreampDelay": "0", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-MinVolts": "0.0000", "ChannelIndex": 3, "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Core-AutoShutter": "1", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "Exposure-ms": 520, "PRIME-PP 2 ENABLED": "Yes", "640 Shutter-TriggerSampleRate": "100000", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "470 Shutter-IOChannel": "Dev1/ao10", "405 AO-Demo": "No", "PixelSizeUm": 1.1, "470 AO-Demo": "No", "470 Shutter-MaxVolts": "5.0000", "Vincent-D3-Name": "Vincent-D3", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FileName": "img_000000000_Il-C_005.tif", "LudlController-Reset": "Operate", "PRIME-Exposure": "520.00", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "445-Name": "DA Shutter", "555 Shutter-Name": "AnalogIO", "520 Shutter-TriggerSampleRate": "100000", "445 Shutter-IOChannel": "Dev1/ao9", "PRIME-TransposeCorrection": "0", "XYStage-StepSize": "0.1000", "445-Description": "DA device that is used as a shutter", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 AO-IOChannel": "Dev1/ao7", "520 Shutter-Name": "AnalogIO", "COM3-DataBits": "8", "640 AO-Volts": "4.5000", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "555 Shutter-IOChannel": "Dev1/ao13", "WaitInterval": null, "520 Shutter-Sequenceable": "1", "YPositionUm": 31506.2, "445-State": "0", "445-DA Device": "445 Shutter", "PRIME-CircularBufferAutoSize": "ON", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "555 AO-TriggerSampleRate": "100000", "445 Shutter-Volts": "0.0000", "XLIGHT Hub-Port": "COM3", "Core-AutoFocus": "", "COM4-Handshaking": "Off", "528 Shutter-MinVolts": "0.0000", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "445 Shutter-Name": "AnalogIO", "528 Shutter-Sequenceable": "1", "XYStage-TransposeMirrorX": "0", "XYStage-TransposeMirrorY": "0", "PRIME-ReadoutRate": "100MHz 16bit", "XYStage-Name": "Stage", "640 AO-SupportsTriggering": "0", "640-Name": "DA Shutter", "640 Shutter-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "640 AO-Block voltage": "false", "445 Shutter-Sequenceable": "1", "470 AO-MaxVolts": "5.0000", "COM4-BaudRate": "9600", "470-Description": "DA device that is used as a shutter", "520 Shutter-Description": "NI DAC", "PRIME-CCDTemperature": "-14.8500", "Time": "2019-12-18 21:37:05 -0500", "640 AO-TriggerSequenceLength": "1000", "470 AO-Name": "AnalogIO", "XLIGHT Disk slider-Label": "Disk Pos 40um", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "Binning": "1x1", "TE2000-ModelType": "1", "COM3-AnswerTimeout": "500.0000", "555 Shutter-SupportsTriggering": "0", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "TE2000-Port": "COM4", "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Description": "NI DAC", "COM4-AnswerTimeout": "500.0000", "COM1-DelayBetweenCharsMs": "0.0000", "FilterBlock-Label": "Confocal", "445 AO-Name": "AnalogIO", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-Timing-ReadoutTimeNs": "24672000.0000", "528 AO-Demo": "No", "520 AO-Sequenceable": "1", "555-Description": "DA device that is used as a shutter", "405-DA Device": "405 Shutter", "405 AO-TriggerSequenceLength": "1000", "640 AO-MinVolts": "0.0000", "640 Shutter-Block voltage": "false", "FrameIndex": 0, "PRIME-Port": "CMOS", "555 AO-MinVolts": "0.0000", "COM3-Description": "Serial port driver (boost:asio)", "528 AO-Description": "NI DAC", "555 AO-IOChannel": "Dev1/ao6", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "555 AO-TriggerInputLine": "/Dev1/PFI0", "PRIME-ActualInterval-ms": "390.5200", "LudlController-Port": "COM9", "445 Shutter-Demo": "No", "PRIME-Trigger-Expose Out-Mux": "1", "SliceIndex": 5, "PRIME-CCDTemperatureSetPoint": "-15.0000", "405 Shutter-MinVolts": "0.0000", "PRIME-ExposeOutMode": "First Row", "AxisPositions": null, "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Volts": "0.0000", "528-Name": "DA Shutter", "555-State": "0", "XLIGHT Dichroic-Label": "Di03-R635-t1", "445 AO-Sequenceable": "1", "405-Name": "DA Shutter", "405 AO-IOChannel": "Dev1/ao0", "Core-Camera": "PRIME", "Focus-Name": "Focus", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "405 Shutter-IOChannel": "Dev1/ao8", "640 AO-Demo": "No", "640 Shutter-SupportsTriggering": "0", "445 AO-Demo": "No", "555-DA Device": "555 Shutter", "470 Shutter-Description": "NI DAC", "528 AO-Volts": "0.0000", "COM1-DataBits": "8", "Vincent-D3-Address": "x", "520 AO-SupportsTriggering": "0", "405-Description": "DA device that is used as a shutter", "640 Shutter-MinVolts": "0.0000", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "405 AO-Name": "AnalogIO", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "640 AO-TriggerInputLine": "/Dev1/PFI0", "405 AO-TriggerInputLine": "/Dev1/PFI0", "640-Description": "DA device that is used as a shutter", "445 Shutter-TriggerSampleRate": "100000", "470 Shutter-TriggerSequenceLength": "1000", "COM1-StopBits": "1", "520 Shutter-SupportsTriggering": "0", "405-State": "0", "Core-Initialize": "1", "XLIGHT Emission Wheel-State": "3", "520 AO-Description": "NI DAC", "528 AO-TriggerInputLine": "/Dev1/PFI0", "445 Shutter-MaxVolts": "5.0000", "COM9-Handshaking": "Off", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "XLIGHT Spin motor-Label": "On (spinning)", "PRIME-PP 5 ENABLED": "No", "UUID": "e6229240-9f5a-4a87-8218-da7ae98f3251", "PRIME-TriggerMode": "Internal Trigger", "COM1-Handshaking": "Off", "PRIME-Timing-PostTriggerDelayNs": "49344000.0000", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "Focus-Position": "17741.5000", "528 Shutter-Block voltage": "false", "Channel": "Il-C", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "555 Shutter-Demo": "No", "405 Shutter-Demo": "No", "528 AO-Name": "AnalogIO", "520-State": "0", "Core-Galvo": "", "405 Shutter-TriggerSampleRate": "100000", "Focus-FocusStepSize_(nm)": "100", "PRIME-PixelType": "16bit", "COM4-DataBits": "8", "470 AO-IOChannel": "Dev1/ao2", "520 AO-MinVolts": "0.0000", "COM9-Parity": "None", "PRIME-CircularBufferEnabled": "ON", "PRIME-PP 6 Reset": "No", "640 AO-Description": "NI DAC", "Source": "PRIME", "445 AO-MinVolts": "0.0000", "PIEZO-Name": "Stage", "445 AO-Description": "NI DAC", "PRIME-CircularBufferFrameCount": "46", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "520 AO-Volts": "0.0000", "528 Shutter-TriggerSampleRate": "100000", "XPositionUm": 19731.9, "OpticalPath-State": "4", "PRIME-PP 3 THRESHOLD": "80", "PRIME-PVCAM Version": "3.7.1", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Gain": "1-HDR", "640 Shutter-TriggerSequenceLength": "1000", "528 AO-SupportsTriggering": "0", "445 AO-TriggerInputLine": "/Dev1/PFI0", "OpticalPath-Name": "OpticalPath", "PRIME-PP 4 ENABLED": "Yes", "PRIME-PVCAM Adapter Version": "1.3.29", "COM3-Name": "COM3", "405 AO-MaxVolts": "5.0000", "520 Shutter-Block voltage": "false", "PRIME-Timing-ClearingTimeNs": "49344000.0000", "Core-Focus": "PIEZO", "528 AO-Block voltage": "false", "TE2000-Description": "Nikon TE2000 hub", "528 AO-MinVolts": "0.0000", "COM4-Parity": "None", "Summary": { "Slices": 11, "ChContrastMin": [ 0, 0, 0, 0, 0, 0 ], "PixelAspect": 1, "SlicesFirst": true, "Interval_ms": 0, "Time": "2019-12-18 21:36:42 -0500", "Prefix": "seq_4", "InitialPositionList": [ { "Label": "Pos0", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.21], "Focus": [17741.5], "XYStage": [ 44542.200000000004, 31636.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos1", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.22], "Focus": [17741.4], "XYStage": [ 43801.100000000006, 31637.9 ] }, "GridColumnIndex": 0 }, { "Label": "Pos2", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.5], "XYStage": [ 38561.3, 32040.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos3", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.185], "Focus": [17741.3], "XYStage": [ 37698.4, 32041.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos4", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.16], "Focus": [17741.5], "XYStage": [ 32375.4, 31758.9 ] }, "GridColumnIndex": 0 }, { "Label": "Pos5", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.17000000000002], "Focus": [17741.3], "XYStage": [ 31770.7, 31758.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos6", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.4], "XYStage": [ 26536.800000000003, 31068.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos7", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.205], "Focus": [17741.4], "XYStage": [ 25420.2, 31068.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos8", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.85], "Focus": [17741.3], "XYStage": [ 21059, 31508 ] }, "GridColumnIndex": 0 }, { "Label": "Pos9", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.82], "Focus": [17741.3], "XYStage": [ 19731.9, 31506.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos10", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.79], "Focus": [17741.4], "XYStage": [ 14528.5, 32007.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos11", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.83], "Focus": [17741.2], "XYStage": [ 13830.300000000001, 32009.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos12", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.79], "Focus": [17741.3], "XYStage": [ 8775.300000000001, 32017.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos13", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.815], "Focus": [17741.3], "XYStage": [ 8109.200000000001, 32008.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos14", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.805], "Focus": [17741.3], "XYStage": [ 3483.3, 31908.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos15", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.82], "Focus": [17741.2], "XYStage": [ 2686.8, 31903.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos16", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [211.48000000000002], "Focus": [17741.1], "XYStage": [ -1978.9, 31973.600000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos17", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [211.51500000000001], "Focus": [17741.1], "XYStage": [ -2957.8, 31982.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos18", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.195], "Focus": [17741.5], "XYStage": [ 47747.4, -576.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos19", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.23000000000002], "Focus": [17741.6], "XYStage": [ 47053.9, -576.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos20", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.205], "Focus": [17741.4], "XYStage": [ 41871.200000000004, -479.8 ] }, "GridColumnIndex": 0 }, { "Label": "Pos21", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.4], "XYStage": [ 40882.600000000006, -392 ] }, "GridColumnIndex": 0 }, { "Label": "Pos22", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.24], "Focus": [17741.4], "XYStage": [ 35905.6, -135.3 ] }, "GridColumnIndex": 0 }, { "Label": "Pos23", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.195], "Focus": [17741.5], "XYStage": [ 34990.9, -132.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos24", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.46], "Focus": [17741.4], "XYStage": [ 29678.100000000002, -958.1 ] }, "GridColumnIndex": 0 }, { "Label": "Pos25", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.44], "Focus": [17741.5], "XYStage": [ 28983.600000000002, -939.4000000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos26", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.475], "Focus": [17741.5], "XYStage": [ 23019.7, -122 ] }, "GridColumnIndex": 0 }, { "Label": "Pos27", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.44], "Focus": [17741.7], "XYStage": [ 22428, -116.80000000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos28", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.46], "Focus": [17741.7], "XYStage": [ 16364.900000000001, -181.10000000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos29", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.48000000000002], "Focus": [17741.5], "XYStage": [ 15712.1, -180 ] }, "GridColumnIndex": 0 }, { "Label": "Pos30", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.45000000000002], "Focus": [17741.7], "XYStage": [ 10346.1, 77.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos31", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.49], "Focus": [17741.4], "XYStage": [ 9755.5, 85.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos32", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.475], "Focus": [17741.5], "XYStage": [ 4775.5, 293.90000000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos33", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.445], "Focus": [17741.4], "XYStage": [ 3954.9, 293.90000000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos34", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.455], "Focus": [17741.4], "XYStage": [ -1324.9, 545.6 ] }, "GridColumnIndex": 0 }, { "Label": "Pos35", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.445], "Focus": [17741.3], "XYStage": [ -2097, 576.6 ] }, "GridColumnIndex": 0 } ], "z-step_um": 10, "GridRow": 0, "BitDepth": 16, "Depth": 2, "Channels": 6, "Source": "Micro-Manager", "ChColors": [ -16711681, -256, -65281, -1, -1, -16776961 ], "MetadataVersion": 10, "KeepShutterOpenChannels": true, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536, 65536 ], "CustomIntervals_ms": [], "Height": 1200, "MicroManagerVersion": "1.4.23 20180219", "GridColumn": 0, "UUID": "14a13bf7-c944-4672-b873-fcfbbb7ea13d", "PixelType": "GRAY16", "Width": 1200, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\Yan\\20191218-YC113-S7S8-miseq", "Frames": 1, "Comment": "", "UserName": "Nikon", "PixelSize_um": 1.1, "ROI": [ 0, 0, 1200, 1200 ], "Date": "2019-12-18", "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC", "P-DAPI-high" ], "KeepShutterOpenSlices": true, "IJType": 1, "ComputerName": "ZadorConfocal2", "Positions": 36, "PositionIndex": 9, "CameraTimeout": 20000, "TimeFirst": false }, "640 AO-Name": "AnalogIO", "Vincent-D3-Command": "Close" }, "FrameKey-0-3-6": { "COM4-StopBits": "1", "528 AO-MaxVolts": "5.0000", "XLIGHT Excitation Wheel-State": "1", "470 Shutter-Name": "AnalogIO", "Core-Shutter": "640", "555 AO-Demo": "No", "XLIGHT Dichroic-State": "2", "XYStage-StepSize-Y": "0.1000", "ZPositionUm": 258.82, "XYStage-StepSize-X": "0.1000", "PRIME-FirmwareVersion": "20.28", "PRIME-ClearCycles": "2", "520-Name": "DA Shutter", "405 Shutter-Name": "AnalogIO", "PIEZO-StepSize": "0.0050", "PRIME-ClearMode": "Pre-Exposure", "470 AO-MinVolts": "0.0000", "405 AO-SupportsTriggering": "1", "CameraChannelIndex": 0, "XYStage-StartSpeed": "500.0000", "COM9-StopBits": "2", "405 AO-Description": "NI DAC", "COM4-DelayBetweenCharsMs": "0.0000", "445 AO-Block voltage": "false", "520 AO-TriggerInputLine": "/Dev1/PFI0", "555 AO-Description": "NI DAC", "520 AO-TriggerSampleRate": "100000", "COM4-Verbose": "1", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM4-Name": "COM4", "405 Shutter-Volts": "0.0000", "Vincent-D3-Port": "COM1", "PRIME-PP 4 MIN ADU AFFECTED": "200", "470 Shutter-MinVolts": "0.0000", "PRIME-PP 3 ENABLED": "Yes", "470 AO-Description": "NI DAC", "PRIME-Description": "PVCAM API device adapter", "520 Shutter-MaxVolts": "5.0000", "520 Shutter-Volts": "0.0000", "528 AO-Sequenceable": "1", "PRIME-Binning": "1x1", "555 Shutter-MaxVolts": "5.0000", "528 Shutter-TriggerSequenceLength": "1000", "405 Shutter-Block voltage": "false", "XLIGHT Spin motor-State": "1", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "Vincent-D3-Time to open (ms)": "35.0000", "Vincent-D3-Time to close (ms)": "35.0000", "445 AO-TriggerSampleRate": "100000", "640 Shutter-IOChannel": "Dev1/ao14", "555 AO-Sequenceable": "1", "520 AO-Name": "AnalogIO", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "470 AO-Block voltage": "false", "COM1-Verbose": "1", "COM9-AnswerTimeout": "10000.0000", "528 Shutter-Name": "AnalogIO", "PRIME-Trigger Timeout (secs)": "10", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "NextFrame": 0, "470 AO-TriggerSequenceLength": "1000", "PRIME-TransposeXY": "0", "445 AO-TriggerSequenceLength": "1000", "COM1-Name": "COM1", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "COM9-DataBits": "8", "COM1-AnswerTimeout": "500.0000", "Camera": "", "TE2000-Version": "V2.50-V2.50-V2.10", "528 Shutter-Volts": "0.0000", "XLIGHT Hub-Name": "XLIGHT Hub", "PIEZO-Description": "Ludl stage driver adapter", "PositionName": "Pos9", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSampleRate": "100000", "PRIME-FanSpeedSetpoint": "High", "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "405 AO-TriggerSampleRate": "100000", "470 Shutter-Block voltage": "false", "470-State": "0", "COM3-Parity": "None", "405 AO-Volts": "0.0000", "PRIME-TransposeMirrorY": "0", "PRIME-ChipName": "GS144BSI", "470 AO-Volts": "0.0000", "PRIME-TransposeMirrorX": "0", "445 Shutter-SupportsTriggering": "0", "470 Shutter-SupportsTriggering": "0", "XLIGHT Hub-Description": "XLIGHT hub", "PRIME-PreampOffLimit": "10000", "470 AO-Sequenceable": "1", "528 Shutter-MaxVolts": "5.0000", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "Core-XYStage": "XYStage", "470 AO-TriggerSampleRate": "100000", "PRIME-Timing-PreTriggerDelayNs": "24672000.0000", "528-DA Device": "528 Shutter", "Focus-Description": "Nikon TE2000 focus adapter", "FilterBlock-State": "5", "520 AO-Demo": "No", "COM3-DelayBetweenCharsMs": "0.0000", "470 Shutter-Volts": "0.0000", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "PRIME-PP 2 THRESHOLD": "125", "PRIME-PP 4 THRESHOLD": "75", "XYStage-Acceleration": "4.0000", "470 Shutter-Demo": "No", "PRIME-Name": "PMPCIECam00", "640-DA Device": "640 Shutter", "405 AO-Block voltage": "false", "ElapsedTime-ms": 398448, "PRIME-Offset": "100", "445 AO-IOChannel": "Dev1/ao1", "640 AO-MaxVolts": "5.0000", "PositionIndex": 9, "PRIME-PP 1 ENABLED": "Yes", "COM1-BaudRate": "9600", "640-State": "1", "COM3-Verbose": "1", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "LudlController-TransmissionDelay": "0", "PRIME-FullWellCapacity": "62000", "PRIME-SMARTStreamingEnabled": "No", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "640 Shutter-Description": "NI DAC", "528-Description": "DA device that is used as a shutter", "COM4-Description": "Serial port driver (boost:asio)", "405 Shutter-Description": "NI DAC", "TE2000-Name": "TE2000", "528 Shutter-Description": "NI DAC", "COM9-Description": "Serial port driver (boost:asio)", "LudlController-Name": "LudlController", "445 Shutter-TriggerSequenceLength": "1000", "PRIME-SerialNumber": "A18B203008", "445 AO-Volts": "0.0000", "PRIME-X-dimension": "1200", "PRIME-ShutterCloseDelay": "0", "XLIGHT Disk slider-State": "2", "Position": "Default", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 AO-IOChannel": "Dev1/ao5", "520 AO-IOChannel": "Dev1/ao4", "528 AO-TriggerSequenceLength": "1000", "405 Shutter-MaxVolts": "5.0000", "470-DA Device": "470 Shutter", "PRIME-FTCapable": "No", "Core-ChannelGroup": "Channels", "555 AO-SupportsTriggering": "0", "PRIME-Y-dimension": "1200", "Frame": 0, "555 Shutter-Sequenceable": "1", "PRIME-Timing-ExposureTimeNs": "499998000.0000", "Core-ImageProcessor": "", "528-State": "0", "520 AO-Block voltage": "false", "XLIGHT Excitation Wheel-Label": "QUAD", "640 Shutter-Demo": "No", "COM3-StopBits": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "520 Shutter-IOChannel": "Dev1/ao11", "Width": 1200, "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-SupportsTriggering": "0", "640 AO-TriggerSampleRate": "100000", "520-Description": "DA device that is used as a shutter", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "405 Shutter-Sequenceable": "1", "405 Shutter-SupportsTriggering": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-TriggerSampleRate": "100000", "640 Shutter-MaxVolts": "5.0000", "555 AO-MaxVolts": "5.0000", "520 AO-TriggerSequenceLength": "1000", "COM3-BaudRate": "9600", "COM9-BaudRate": "9600", "Vincent-D3-Channel #": "Ch. #1", "PRIME-PMode": "Normal", "OpticalPath-Label": "Left(100)", "BitDepth": 16, "555 AO-TriggerSequenceLength": "1000", "445 Shutter-Description": "NI DAC", "405 AO-Sequenceable": "1", "PRIME-AcquisitionMethod": "Callbacks", "555 AO-Name": "AnalogIO", "PRIME-ShutterMode": "Pre-Exposure", "COM3-Handshaking": "Off", "445 AO-SupportsTriggering": "0", "ROI": "0-0-1200-1200", "COM9-Verbose": "1", "PRIME-Color": "OFF", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "PRIME-ShutterOpenDelay": "0", "COM1-Parity": "None", "Slice": 6, "SlicePosition": 10, "COM9-DelayBetweenCharsMs": "0.0000", "555-Name": "DA Shutter", "PIEZO-LudlSingleAxisName": "B", "XLIGHT Emission Wheel-Label": "725/40 (C)", "640 Shutter-Name": "AnalogIO", "640 AO-Sequenceable": "1", "520 Shutter-Demo": "No", "528 Shutter-Demo": "No", "470-Name": "DA Shutter", "520-DA Device": "520 Shutter", "XYStage-Description": "Ludl XY stage driver adapter", "445 Shutter-MinVolts": "0.0000", "528 AO-TriggerSampleRate": "100000", "405 AO-MinVolts": "0.0000", "405 Shutter-TriggerSequenceLength": "1000", "Height": 1200, "555 AO-Block voltage": "false", "640 Shutter-Volts": "5.0000", "PixelType": "GRAY16", "445 Shutter-Block voltage": "false", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "520 Shutter-TriggerSequenceLength": "1000", "COM9-Name": "COM9", "Core-SLM": "", "555 AO-Volts": "0.0000", "555 Shutter-Block voltage": "false", "XYStage-Speed": "1800.0000", "520 AO-MaxVolts": "5.0000", "528 Shutter-SupportsTriggering": "0", "COM1-Description": "Serial port driver (boost:asio)", "PRIME-MetadataEnabled": "No", "520 Shutter-MinVolts": "0.0000", "470 Shutter-Sequenceable": "1", "PRIME-PreampDelay": "0", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-MinVolts": "0.0000", "ChannelIndex": 3, "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Core-AutoShutter": "1", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "Exposure-ms": 520, "PRIME-PP 2 ENABLED": "Yes", "640 Shutter-TriggerSampleRate": "100000", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "470 Shutter-IOChannel": "Dev1/ao10", "405 AO-Demo": "No", "PixelSizeUm": 1.1, "470 AO-Demo": "No", "470 Shutter-MaxVolts": "5.0000", "Vincent-D3-Name": "Vincent-D3", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FileName": "img_000000000_Il-C_006.tif", "LudlController-Reset": "Operate", "PRIME-Exposure": "520.00", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "445-Name": "DA Shutter", "555 Shutter-Name": "AnalogIO", "520 Shutter-TriggerSampleRate": "100000", "445 Shutter-IOChannel": "Dev1/ao9", "PRIME-TransposeCorrection": "0", "XYStage-StepSize": "0.1000", "445-Description": "DA device that is used as a shutter", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 AO-IOChannel": "Dev1/ao7", "520 Shutter-Name": "AnalogIO", "COM3-DataBits": "8", "640 AO-Volts": "4.5000", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "555 Shutter-IOChannel": "Dev1/ao13", "WaitInterval": null, "520 Shutter-Sequenceable": "1", "YPositionUm": 31506.2, "445-State": "0", "445-DA Device": "445 Shutter", "PRIME-CircularBufferAutoSize": "ON", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "555 AO-TriggerSampleRate": "100000", "445 Shutter-Volts": "0.0000", "XLIGHT Hub-Port": "COM3", "Core-AutoFocus": "", "COM4-Handshaking": "Off", "528 Shutter-MinVolts": "0.0000", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "445 Shutter-Name": "AnalogIO", "528 Shutter-Sequenceable": "1", "XYStage-TransposeMirrorX": "0", "XYStage-TransposeMirrorY": "0", "PRIME-ReadoutRate": "100MHz 16bit", "XYStage-Name": "Stage", "640 AO-SupportsTriggering": "0", "640-Name": "DA Shutter", "640 Shutter-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "640 AO-Block voltage": "false", "445 Shutter-Sequenceable": "1", "470 AO-MaxVolts": "5.0000", "COM4-BaudRate": "9600", "470-Description": "DA device that is used as a shutter", "520 Shutter-Description": "NI DAC", "PRIME-CCDTemperature": "-14.8500", "Time": "2019-12-18 21:37:06 -0500", "640 AO-TriggerSequenceLength": "1000", "470 AO-Name": "AnalogIO", "XLIGHT Disk slider-Label": "Disk Pos 40um", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "Binning": "1x1", "TE2000-ModelType": "1", "COM3-AnswerTimeout": "500.0000", "555 Shutter-SupportsTriggering": "0", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "TE2000-Port": "COM4", "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Description": "NI DAC", "COM4-AnswerTimeout": "500.0000", "COM1-DelayBetweenCharsMs": "0.0000", "FilterBlock-Label": "Confocal", "445 AO-Name": "AnalogIO", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-Timing-ReadoutTimeNs": "24672000.0000", "528 AO-Demo": "No", "520 AO-Sequenceable": "1", "555-Description": "DA device that is used as a shutter", "405-DA Device": "405 Shutter", "405 AO-TriggerSequenceLength": "1000", "640 AO-MinVolts": "0.0000", "640 Shutter-Block voltage": "false", "FrameIndex": 0, "PRIME-Port": "CMOS", "555 AO-MinVolts": "0.0000", "COM3-Description": "Serial port driver (boost:asio)", "528 AO-Description": "NI DAC", "555 AO-IOChannel": "Dev1/ao6", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "555 AO-TriggerInputLine": "/Dev1/PFI0", "PRIME-ActualInterval-ms": "390.5200", "LudlController-Port": "COM9", "445 Shutter-Demo": "No", "PRIME-Trigger-Expose Out-Mux": "1", "SliceIndex": 6, "PRIME-CCDTemperatureSetPoint": "-15.0000", "405 Shutter-MinVolts": "0.0000", "PRIME-ExposeOutMode": "First Row", "AxisPositions": null, "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Volts": "0.0000", "528-Name": "DA Shutter", "555-State": "0", "XLIGHT Dichroic-Label": "Di03-R635-t1", "445 AO-Sequenceable": "1", "405-Name": "DA Shutter", "405 AO-IOChannel": "Dev1/ao0", "Core-Camera": "PRIME", "Focus-Name": "Focus", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "405 Shutter-IOChannel": "Dev1/ao8", "640 AO-Demo": "No", "640 Shutter-SupportsTriggering": "0", "445 AO-Demo": "No", "555-DA Device": "555 Shutter", "470 Shutter-Description": "NI DAC", "528 AO-Volts": "0.0000", "COM1-DataBits": "8", "Vincent-D3-Address": "x", "520 AO-SupportsTriggering": "0", "405-Description": "DA device that is used as a shutter", "640 Shutter-MinVolts": "0.0000", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "405 AO-Name": "AnalogIO", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "640 AO-TriggerInputLine": "/Dev1/PFI0", "405 AO-TriggerInputLine": "/Dev1/PFI0", "640-Description": "DA device that is used as a shutter", "445 Shutter-TriggerSampleRate": "100000", "470 Shutter-TriggerSequenceLength": "1000", "COM1-StopBits": "1", "520 Shutter-SupportsTriggering": "0", "405-State": "0", "Core-Initialize": "1", "XLIGHT Emission Wheel-State": "3", "520 AO-Description": "NI DAC", "528 AO-TriggerInputLine": "/Dev1/PFI0", "445 Shutter-MaxVolts": "5.0000", "COM9-Handshaking": "Off", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "XLIGHT Spin motor-Label": "On (spinning)", "PRIME-PP 5 ENABLED": "No", "UUID": "389f3a7d-249a-4de0-b532-2c1135258d11", "PRIME-TriggerMode": "Internal Trigger", "COM1-Handshaking": "Off", "PRIME-Timing-PostTriggerDelayNs": "49344000.0000", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "Focus-Position": "17741.5000", "528 Shutter-Block voltage": "false", "Channel": "Il-C", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "555 Shutter-Demo": "No", "405 Shutter-Demo": "No", "528 AO-Name": "AnalogIO", "520-State": "0", "Core-Galvo": "", "405 Shutter-TriggerSampleRate": "100000", "Focus-FocusStepSize_(nm)": "100", "PRIME-PixelType": "16bit", "COM4-DataBits": "8", "470 AO-IOChannel": "Dev1/ao2", "520 AO-MinVolts": "0.0000", "COM9-Parity": "None", "PRIME-CircularBufferEnabled": "ON", "PRIME-PP 6 Reset": "No", "640 AO-Description": "NI DAC", "Source": "PRIME", "445 AO-MinVolts": "0.0000", "PIEZO-Name": "Stage", "445 AO-Description": "NI DAC", "PRIME-CircularBufferFrameCount": "46", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "520 AO-Volts": "0.0000", "528 Shutter-TriggerSampleRate": "100000", "XPositionUm": 19731.9, "OpticalPath-State": "4", "PRIME-PP 3 THRESHOLD": "80", "PRIME-PVCAM Version": "3.7.1", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Gain": "1-HDR", "640 Shutter-TriggerSequenceLength": "1000", "528 AO-SupportsTriggering": "0", "445 AO-TriggerInputLine": "/Dev1/PFI0", "OpticalPath-Name": "OpticalPath", "PRIME-PP 4 ENABLED": "Yes", "PRIME-PVCAM Adapter Version": "1.3.29", "COM3-Name": "COM3", "405 AO-MaxVolts": "5.0000", "520 Shutter-Block voltage": "false", "PRIME-Timing-ClearingTimeNs": "49344000.0000", "Core-Focus": "PIEZO", "528 AO-Block voltage": "false", "TE2000-Description": "Nikon TE2000 hub", "528 AO-MinVolts": "0.0000", "COM4-Parity": "None", "Summary": { "Slices": 11, "ChContrastMin": [ 0, 0, 0, 0, 0, 0 ], "PixelAspect": 1, "SlicesFirst": true, "Interval_ms": 0, "Time": "2019-12-18 21:36:42 -0500", "Prefix": "seq_4", "InitialPositionList": [ { "Label": "Pos0", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.21], "Focus": [17741.5], "XYStage": [ 44542.200000000004, 31636.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos1", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.22], "Focus": [17741.4], "XYStage": [ 43801.100000000006, 31637.9 ] }, "GridColumnIndex": 0 }, { "Label": "Pos2", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.5], "XYStage": [ 38561.3, 32040.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos3", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.185], "Focus": [17741.3], "XYStage": [ 37698.4, 32041.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos4", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.16], "Focus": [17741.5], "XYStage": [ 32375.4, 31758.9 ] }, "GridColumnIndex": 0 }, { "Label": "Pos5", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.17000000000002], "Focus": [17741.3], "XYStage": [ 31770.7, 31758.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos6", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.4], "XYStage": [ 26536.800000000003, 31068.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos7", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.205], "Focus": [17741.4], "XYStage": [ 25420.2, 31068.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos8", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.85], "Focus": [17741.3], "XYStage": [ 21059, 31508 ] }, "GridColumnIndex": 0 }, { "Label": "Pos9", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.82], "Focus": [17741.3], "XYStage": [ 19731.9, 31506.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos10", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.79], "Focus": [17741.4], "XYStage": [ 14528.5, 32007.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos11", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.83], "Focus": [17741.2], "XYStage": [ 13830.300000000001, 32009.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos12", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.79], "Focus": [17741.3], "XYStage": [ 8775.300000000001, 32017.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos13", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.815], "Focus": [17741.3], "XYStage": [ 8109.200000000001, 32008.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos14", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.805], "Focus": [17741.3], "XYStage": [ 3483.3, 31908.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos15", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.82], "Focus": [17741.2], "XYStage": [ 2686.8, 31903.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos16", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [211.48000000000002], "Focus": [17741.1], "XYStage": [ -1978.9, 31973.600000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos17", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [211.51500000000001], "Focus": [17741.1], "XYStage": [ -2957.8, 31982.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos18", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.195], "Focus": [17741.5], "XYStage": [ 47747.4, -576.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos19", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.23000000000002], "Focus": [17741.6], "XYStage": [ 47053.9, -576.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos20", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.205], "Focus": [17741.4], "XYStage": [ 41871.200000000004, -479.8 ] }, "GridColumnIndex": 0 }, { "Label": "Pos21", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.4], "XYStage": [ 40882.600000000006, -392 ] }, "GridColumnIndex": 0 }, { "Label": "Pos22", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.24], "Focus": [17741.4], "XYStage": [ 35905.6, -135.3 ] }, "GridColumnIndex": 0 }, { "Label": "Pos23", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.195], "Focus": [17741.5], "XYStage": [ 34990.9, -132.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos24", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.46], "Focus": [17741.4], "XYStage": [ 29678.100000000002, -958.1 ] }, "GridColumnIndex": 0 }, { "Label": "Pos25", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.44], "Focus": [17741.5], "XYStage": [ 28983.600000000002, -939.4000000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos26", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.475], "Focus": [17741.5], "XYStage": [ 23019.7, -122 ] }, "GridColumnIndex": 0 }, { "Label": "Pos27", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.44], "Focus": [17741.7], "XYStage": [ 22428, -116.80000000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos28", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.46], "Focus": [17741.7], "XYStage": [ 16364.900000000001, -181.10000000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos29", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.48000000000002], "Focus": [17741.5], "XYStage": [ 15712.1, -180 ] }, "GridColumnIndex": 0 }, { "Label": "Pos30", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.45000000000002], "Focus": [17741.7], "XYStage": [ 10346.1, 77.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos31", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.49], "Focus": [17741.4], "XYStage": [ 9755.5, 85.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos32", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.475], "Focus": [17741.5], "XYStage": [ 4775.5, 293.90000000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos33", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.445], "Focus": [17741.4], "XYStage": [ 3954.9, 293.90000000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos34", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.455], "Focus": [17741.4], "XYStage": [ -1324.9, 545.6 ] }, "GridColumnIndex": 0 }, { "Label": "Pos35", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.445], "Focus": [17741.3], "XYStage": [ -2097, 576.6 ] }, "GridColumnIndex": 0 } ], "z-step_um": 10, "GridRow": 0, "BitDepth": 16, "Depth": 2, "Channels": 6, "Source": "Micro-Manager", "ChColors": [ -16711681, -256, -65281, -1, -1, -16776961 ], "MetadataVersion": 10, "KeepShutterOpenChannels": true, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536, 65536 ], "CustomIntervals_ms": [], "Height": 1200, "MicroManagerVersion": "1.4.23 20180219", "GridColumn": 0, "UUID": "14a13bf7-c944-4672-b873-fcfbbb7ea13d", "PixelType": "GRAY16", "Width": 1200, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\Yan\\20191218-YC113-S7S8-miseq", "Frames": 1, "Comment": "", "UserName": "Nikon", "PixelSize_um": 1.1, "ROI": [ 0, 0, 1200, 1200 ], "Date": "2019-12-18", "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC", "P-DAPI-high" ], "KeepShutterOpenSlices": true, "IJType": 1, "ComputerName": "ZadorConfocal2", "Positions": 36, "PositionIndex": 9, "CameraTimeout": 20000, "TimeFirst": false }, "640 AO-Name": "AnalogIO", "Vincent-D3-Command": "Close" }, "FrameKey-0-3-7": { "COM4-StopBits": "1", "528 AO-MaxVolts": "5.0000", "XLIGHT Excitation Wheel-State": "1", "470 Shutter-Name": "AnalogIO", "Core-Shutter": "640", "555 AO-Demo": "No", "XLIGHT Dichroic-State": "2", "XYStage-StepSize-Y": "0.1000", "ZPositionUm": 268.82, "XYStage-StepSize-X": "0.1000", "PRIME-FirmwareVersion": "20.28", "PRIME-ClearCycles": "2", "520-Name": "DA Shutter", "405 Shutter-Name": "AnalogIO", "PIEZO-StepSize": "0.0050", "PRIME-ClearMode": "Pre-Exposure", "470 AO-MinVolts": "0.0000", "405 AO-SupportsTriggering": "1", "CameraChannelIndex": 0, "XYStage-StartSpeed": "500.0000", "COM9-StopBits": "2", "405 AO-Description": "NI DAC", "COM4-DelayBetweenCharsMs": "0.0000", "445 AO-Block voltage": "false", "520 AO-TriggerInputLine": "/Dev1/PFI0", "555 AO-Description": "NI DAC", "520 AO-TriggerSampleRate": "100000", "COM4-Verbose": "1", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM4-Name": "COM4", "405 Shutter-Volts": "0.0000", "Vincent-D3-Port": "COM1", "PRIME-PP 4 MIN ADU AFFECTED": "200", "470 Shutter-MinVolts": "0.0000", "PRIME-PP 3 ENABLED": "Yes", "470 AO-Description": "NI DAC", "PRIME-Description": "PVCAM API device adapter", "520 Shutter-MaxVolts": "5.0000", "520 Shutter-Volts": "0.0000", "528 AO-Sequenceable": "1", "PRIME-Binning": "1x1", "555 Shutter-MaxVolts": "5.0000", "528 Shutter-TriggerSequenceLength": "1000", "405 Shutter-Block voltage": "false", "XLIGHT Spin motor-State": "1", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "Vincent-D3-Time to open (ms)": "35.0000", "Vincent-D3-Time to close (ms)": "35.0000", "445 AO-TriggerSampleRate": "100000", "640 Shutter-IOChannel": "Dev1/ao14", "555 AO-Sequenceable": "1", "520 AO-Name": "AnalogIO", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "470 AO-Block voltage": "false", "COM1-Verbose": "1", "COM9-AnswerTimeout": "10000.0000", "528 Shutter-Name": "AnalogIO", "PRIME-Trigger Timeout (secs)": "10", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "NextFrame": 0, "470 AO-TriggerSequenceLength": "1000", "PRIME-TransposeXY": "0", "445 AO-TriggerSequenceLength": "1000", "COM1-Name": "COM1", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "COM9-DataBits": "8", "COM1-AnswerTimeout": "500.0000", "Camera": "", "TE2000-Version": "V2.50-V2.50-V2.10", "528 Shutter-Volts": "0.0000", "XLIGHT Hub-Name": "XLIGHT Hub", "PIEZO-Description": "Ludl stage driver adapter", "PositionName": "Pos9", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSampleRate": "100000", "PRIME-FanSpeedSetpoint": "High", "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "405 AO-TriggerSampleRate": "100000", "470 Shutter-Block voltage": "false", "470-State": "0", "COM3-Parity": "None", "405 AO-Volts": "0.0000", "PRIME-TransposeMirrorY": "0", "PRIME-ChipName": "GS144BSI", "470 AO-Volts": "0.0000", "PRIME-TransposeMirrorX": "0", "445 Shutter-SupportsTriggering": "0", "470 Shutter-SupportsTriggering": "0", "XLIGHT Hub-Description": "XLIGHT hub", "PRIME-PreampOffLimit": "10000", "470 AO-Sequenceable": "1", "528 Shutter-MaxVolts": "5.0000", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "Core-XYStage": "XYStage", "470 AO-TriggerSampleRate": "100000", "PRIME-Timing-PreTriggerDelayNs": "24672000.0000", "528-DA Device": "528 Shutter", "Focus-Description": "Nikon TE2000 focus adapter", "FilterBlock-State": "5", "520 AO-Demo": "No", "COM3-DelayBetweenCharsMs": "0.0000", "470 Shutter-Volts": "0.0000", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "PRIME-PP 2 THRESHOLD": "125", "PRIME-PP 4 THRESHOLD": "75", "XYStage-Acceleration": "4.0000", "470 Shutter-Demo": "No", "PRIME-Name": "PMPCIECam00", "640-DA Device": "640 Shutter", "405 AO-Block voltage": "false", "ElapsedTime-ms": 399201, "PRIME-Offset": "100", "445 AO-IOChannel": "Dev1/ao1", "640 AO-MaxVolts": "5.0000", "PositionIndex": 9, "PRIME-PP 1 ENABLED": "Yes", "COM1-BaudRate": "9600", "640-State": "1", "COM3-Verbose": "1", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "LudlController-TransmissionDelay": "0", "PRIME-FullWellCapacity": "62000", "PRIME-SMARTStreamingEnabled": "No", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "640 Shutter-Description": "NI DAC", "528-Description": "DA device that is used as a shutter", "COM4-Description": "Serial port driver (boost:asio)", "405 Shutter-Description": "NI DAC", "TE2000-Name": "TE2000", "528 Shutter-Description": "NI DAC", "COM9-Description": "Serial port driver (boost:asio)", "LudlController-Name": "LudlController", "445 Shutter-TriggerSequenceLength": "1000", "PRIME-SerialNumber": "A18B203008", "445 AO-Volts": "0.0000", "PRIME-X-dimension": "1200", "PRIME-ShutterCloseDelay": "0", "XLIGHT Disk slider-State": "2", "Position": "Default", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 AO-IOChannel": "Dev1/ao5", "520 AO-IOChannel": "Dev1/ao4", "528 AO-TriggerSequenceLength": "1000", "405 Shutter-MaxVolts": "5.0000", "470-DA Device": "470 Shutter", "PRIME-FTCapable": "No", "Core-ChannelGroup": "Channels", "555 AO-SupportsTriggering": "0", "PRIME-Y-dimension": "1200", "Frame": 0, "555 Shutter-Sequenceable": "1", "PRIME-Timing-ExposureTimeNs": "499998000.0000", "Core-ImageProcessor": "", "528-State": "0", "520 AO-Block voltage": "false", "XLIGHT Excitation Wheel-Label": "QUAD", "640 Shutter-Demo": "No", "COM3-StopBits": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "520 Shutter-IOChannel": "Dev1/ao11", "Width": 1200, "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-SupportsTriggering": "0", "640 AO-TriggerSampleRate": "100000", "520-Description": "DA device that is used as a shutter", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "405 Shutter-Sequenceable": "1", "405 Shutter-SupportsTriggering": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-TriggerSampleRate": "100000", "640 Shutter-MaxVolts": "5.0000", "555 AO-MaxVolts": "5.0000", "520 AO-TriggerSequenceLength": "1000", "COM3-BaudRate": "9600", "COM9-BaudRate": "9600", "Vincent-D3-Channel #": "Ch. #1", "PRIME-PMode": "Normal", "OpticalPath-Label": "Left(100)", "BitDepth": 16, "555 AO-TriggerSequenceLength": "1000", "445 Shutter-Description": "NI DAC", "405 AO-Sequenceable": "1", "PRIME-AcquisitionMethod": "Callbacks", "555 AO-Name": "AnalogIO", "PRIME-ShutterMode": "Pre-Exposure", "COM3-Handshaking": "Off", "445 AO-SupportsTriggering": "0", "ROI": "0-0-1200-1200", "COM9-Verbose": "1", "PRIME-Color": "OFF", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "PRIME-ShutterOpenDelay": "0", "COM1-Parity": "None", "Slice": 7, "SlicePosition": 20, "COM9-DelayBetweenCharsMs": "0.0000", "555-Name": "DA Shutter", "PIEZO-LudlSingleAxisName": "B", "XLIGHT Emission Wheel-Label": "725/40 (C)", "640 Shutter-Name": "AnalogIO", "640 AO-Sequenceable": "1", "520 Shutter-Demo": "No", "528 Shutter-Demo": "No", "470-Name": "DA Shutter", "520-DA Device": "520 Shutter", "XYStage-Description": "Ludl XY stage driver adapter", "445 Shutter-MinVolts": "0.0000", "528 AO-TriggerSampleRate": "100000", "405 AO-MinVolts": "0.0000", "405 Shutter-TriggerSequenceLength": "1000", "Height": 1200, "555 AO-Block voltage": "false", "640 Shutter-Volts": "5.0000", "PixelType": "GRAY16", "445 Shutter-Block voltage": "false", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "520 Shutter-TriggerSequenceLength": "1000", "COM9-Name": "COM9", "Core-SLM": "", "555 AO-Volts": "0.0000", "555 Shutter-Block voltage": "false", "XYStage-Speed": "1800.0000", "520 AO-MaxVolts": "5.0000", "528 Shutter-SupportsTriggering": "0", "COM1-Description": "Serial port driver (boost:asio)", "PRIME-MetadataEnabled": "No", "520 Shutter-MinVolts": "0.0000", "470 Shutter-Sequenceable": "1", "PRIME-PreampDelay": "0", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-MinVolts": "0.0000", "ChannelIndex": 3, "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Core-AutoShutter": "1", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "Exposure-ms": 520, "PRIME-PP 2 ENABLED": "Yes", "640 Shutter-TriggerSampleRate": "100000", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "470 Shutter-IOChannel": "Dev1/ao10", "405 AO-Demo": "No", "PixelSizeUm": 1.1, "470 AO-Demo": "No", "470 Shutter-MaxVolts": "5.0000", "Vincent-D3-Name": "Vincent-D3", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FileName": "img_000000000_Il-C_007.tif", "LudlController-Reset": "Operate", "PRIME-Exposure": "520.00", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "445-Name": "DA Shutter", "555 Shutter-Name": "AnalogIO", "520 Shutter-TriggerSampleRate": "100000", "445 Shutter-IOChannel": "Dev1/ao9", "PRIME-TransposeCorrection": "0", "XYStage-StepSize": "0.1000", "445-Description": "DA device that is used as a shutter", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 AO-IOChannel": "Dev1/ao7", "520 Shutter-Name": "AnalogIO", "COM3-DataBits": "8", "640 AO-Volts": "4.5000", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "555 Shutter-IOChannel": "Dev1/ao13", "WaitInterval": null, "520 Shutter-Sequenceable": "1", "YPositionUm": 31506.2, "445-State": "0", "445-DA Device": "445 Shutter", "PRIME-CircularBufferAutoSize": "ON", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "555 AO-TriggerSampleRate": "100000", "445 Shutter-Volts": "0.0000", "XLIGHT Hub-Port": "COM3", "Core-AutoFocus": "", "COM4-Handshaking": "Off", "528 Shutter-MinVolts": "0.0000", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "445 Shutter-Name": "AnalogIO", "528 Shutter-Sequenceable": "1", "XYStage-TransposeMirrorX": "0", "XYStage-TransposeMirrorY": "0", "PRIME-ReadoutRate": "100MHz 16bit", "XYStage-Name": "Stage", "640 AO-SupportsTriggering": "0", "640-Name": "DA Shutter", "640 Shutter-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "640 AO-Block voltage": "false", "445 Shutter-Sequenceable": "1", "470 AO-MaxVolts": "5.0000", "COM4-BaudRate": "9600", "470-Description": "DA device that is used as a shutter", "520 Shutter-Description": "NI DAC", "PRIME-CCDTemperature": "-14.8500", "Time": "2019-12-18 21:37:07 -0500", "640 AO-TriggerSequenceLength": "1000", "470 AO-Name": "AnalogIO", "XLIGHT Disk slider-Label": "Disk Pos 40um", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "Binning": "1x1", "TE2000-ModelType": "1", "COM3-AnswerTimeout": "500.0000", "555 Shutter-SupportsTriggering": "0", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "TE2000-Port": "COM4", "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Description": "NI DAC", "COM4-AnswerTimeout": "500.0000", "COM1-DelayBetweenCharsMs": "0.0000", "FilterBlock-Label": "Confocal", "445 AO-Name": "AnalogIO", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-Timing-ReadoutTimeNs": "24672000.0000", "528 AO-Demo": "No", "520 AO-Sequenceable": "1", "555-Description": "DA device that is used as a shutter", "405-DA Device": "405 Shutter", "405 AO-TriggerSequenceLength": "1000", "640 AO-MinVolts": "0.0000", "640 Shutter-Block voltage": "false", "FrameIndex": 0, "PRIME-Port": "CMOS", "555 AO-MinVolts": "0.0000", "COM3-Description": "Serial port driver (boost:asio)", "528 AO-Description": "NI DAC", "555 AO-IOChannel": "Dev1/ao6", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "555 AO-TriggerInputLine": "/Dev1/PFI0", "PRIME-ActualInterval-ms": "390.5200", "LudlController-Port": "COM9", "445 Shutter-Demo": "No", "PRIME-Trigger-Expose Out-Mux": "1", "SliceIndex": 7, "PRIME-CCDTemperatureSetPoint": "-15.0000", "405 Shutter-MinVolts": "0.0000", "PRIME-ExposeOutMode": "First Row", "AxisPositions": null, "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Volts": "0.0000", "528-Name": "DA Shutter", "555-State": "0", "XLIGHT Dichroic-Label": "Di03-R635-t1", "445 AO-Sequenceable": "1", "405-Name": "DA Shutter", "405 AO-IOChannel": "Dev1/ao0", "Core-Camera": "PRIME", "Focus-Name": "Focus", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "405 Shutter-IOChannel": "Dev1/ao8", "640 AO-Demo": "No", "640 Shutter-SupportsTriggering": "0", "445 AO-Demo": "No", "555-DA Device": "555 Shutter", "470 Shutter-Description": "NI DAC", "528 AO-Volts": "0.0000", "COM1-DataBits": "8", "Vincent-D3-Address": "x", "520 AO-SupportsTriggering": "0", "405-Description": "DA device that is used as a shutter", "640 Shutter-MinVolts": "0.0000", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "405 AO-Name": "AnalogIO", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "640 AO-TriggerInputLine": "/Dev1/PFI0", "405 AO-TriggerInputLine": "/Dev1/PFI0", "640-Description": "DA device that is used as a shutter", "445 Shutter-TriggerSampleRate": "100000", "470 Shutter-TriggerSequenceLength": "1000", "COM1-StopBits": "1", "520 Shutter-SupportsTriggering": "0", "405-State": "0", "Core-Initialize": "1", "XLIGHT Emission Wheel-State": "3", "520 AO-Description": "NI DAC", "528 AO-TriggerInputLine": "/Dev1/PFI0", "445 Shutter-MaxVolts": "5.0000", "COM9-Handshaking": "Off", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "XLIGHT Spin motor-Label": "On (spinning)", "PRIME-PP 5 ENABLED": "No", "UUID": "794c20f3-0299-43bd-a192-9db162b53682", "PRIME-TriggerMode": "Internal Trigger", "COM1-Handshaking": "Off", "PRIME-Timing-PostTriggerDelayNs": "49344000.0000", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "Focus-Position": "17741.5000", "528 Shutter-Block voltage": "false", "Channel": "Il-C", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "555 Shutter-Demo": "No", "405 Shutter-Demo": "No", "528 AO-Name": "AnalogIO", "520-State": "0", "Core-Galvo": "", "405 Shutter-TriggerSampleRate": "100000", "Focus-FocusStepSize_(nm)": "100", "PRIME-PixelType": "16bit", "COM4-DataBits": "8", "470 AO-IOChannel": "Dev1/ao2", "520 AO-MinVolts": "0.0000", "COM9-Parity": "None", "PRIME-CircularBufferEnabled": "ON", "PRIME-PP 6 Reset": "No", "640 AO-Description": "NI DAC", "Source": "PRIME", "445 AO-MinVolts": "0.0000", "PIEZO-Name": "Stage", "445 AO-Description": "NI DAC", "PRIME-CircularBufferFrameCount": "46", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "520 AO-Volts": "0.0000", "528 Shutter-TriggerSampleRate": "100000", "XPositionUm": 19731.9, "OpticalPath-State": "4", "PRIME-PP 3 THRESHOLD": "80", "PRIME-PVCAM Version": "3.7.1", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Gain": "1-HDR", "640 Shutter-TriggerSequenceLength": "1000", "528 AO-SupportsTriggering": "0", "445 AO-TriggerInputLine": "/Dev1/PFI0", "OpticalPath-Name": "OpticalPath", "PRIME-PP 4 ENABLED": "Yes", "PRIME-PVCAM Adapter Version": "1.3.29", "COM3-Name": "COM3", "405 AO-MaxVolts": "5.0000", "520 Shutter-Block voltage": "false", "PRIME-Timing-ClearingTimeNs": "49344000.0000", "Core-Focus": "PIEZO", "528 AO-Block voltage": "false", "TE2000-Description": "Nikon TE2000 hub", "528 AO-MinVolts": "0.0000", "COM4-Parity": "None", "Summary": { "Slices": 11, "ChContrastMin": [ 0, 0, 0, 0, 0, 0 ], "PixelAspect": 1, "SlicesFirst": true, "Interval_ms": 0, "Time": "2019-12-18 21:36:42 -0500", "Prefix": "seq_4", "InitialPositionList": [ { "Label": "Pos0", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.21], "Focus": [17741.5], "XYStage": [ 44542.200000000004, 31636.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos1", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.22], "Focus": [17741.4], "XYStage": [ 43801.100000000006, 31637.9 ] }, "GridColumnIndex": 0 }, { "Label": "Pos2", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.5], "XYStage": [ 38561.3, 32040.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos3", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.185], "Focus": [17741.3], "XYStage": [ 37698.4, 32041.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos4", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.16], "Focus": [17741.5], "XYStage": [ 32375.4, 31758.9 ] }, "GridColumnIndex": 0 }, { "Label": "Pos5", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.17000000000002], "Focus": [17741.3], "XYStage": [ 31770.7, 31758.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos6", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.4], "XYStage": [ 26536.800000000003, 31068.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos7", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.205], "Focus": [17741.4], "XYStage": [ 25420.2, 31068.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos8", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.85], "Focus": [17741.3], "XYStage": [ 21059, 31508 ] }, "GridColumnIndex": 0 }, { "Label": "Pos9", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.82], "Focus": [17741.3], "XYStage": [ 19731.9, 31506.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos10", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.79], "Focus": [17741.4], "XYStage": [ 14528.5, 32007.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos11", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.83], "Focus": [17741.2], "XYStage": [ 13830.300000000001, 32009.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos12", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.79], "Focus": [17741.3], "XYStage": [ 8775.300000000001, 32017.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos13", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.815], "Focus": [17741.3], "XYStage": [ 8109.200000000001, 32008.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos14", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.805], "Focus": [17741.3], "XYStage": [ 3483.3, 31908.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos15", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.82], "Focus": [17741.2], "XYStage": [ 2686.8, 31903.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos16", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [211.48000000000002], "Focus": [17741.1], "XYStage": [ -1978.9, 31973.600000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos17", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [211.51500000000001], "Focus": [17741.1], "XYStage": [ -2957.8, 31982.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos18", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.195], "Focus": [17741.5], "XYStage": [ 47747.4, -576.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos19", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.23000000000002], "Focus": [17741.6], "XYStage": [ 47053.9, -576.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos20", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.205], "Focus": [17741.4], "XYStage": [ 41871.200000000004, -479.8 ] }, "GridColumnIndex": 0 }, { "Label": "Pos21", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.4], "XYStage": [ 40882.600000000006, -392 ] }, "GridColumnIndex": 0 }, { "Label": "Pos22", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.24], "Focus": [17741.4], "XYStage": [ 35905.6, -135.3 ] }, "GridColumnIndex": 0 }, { "Label": "Pos23", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.195], "Focus": [17741.5], "XYStage": [ 34990.9, -132.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos24", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.46], "Focus": [17741.4], "XYStage": [ 29678.100000000002, -958.1 ] }, "GridColumnIndex": 0 }, { "Label": "Pos25", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.44], "Focus": [17741.5], "XYStage": [ 28983.600000000002, -939.4000000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos26", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.475], "Focus": [17741.5], "XYStage": [ 23019.7, -122 ] }, "GridColumnIndex": 0 }, { "Label": "Pos27", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.44], "Focus": [17741.7], "XYStage": [ 22428, -116.80000000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos28", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.46], "Focus": [17741.7], "XYStage": [ 16364.900000000001, -181.10000000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos29", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.48000000000002], "Focus": [17741.5], "XYStage": [ 15712.1, -180 ] }, "GridColumnIndex": 0 }, { "Label": "Pos30", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.45000000000002], "Focus": [17741.7], "XYStage": [ 10346.1, 77.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos31", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.49], "Focus": [17741.4], "XYStage": [ 9755.5, 85.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos32", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.475], "Focus": [17741.5], "XYStage": [ 4775.5, 293.90000000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos33", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.445], "Focus": [17741.4], "XYStage": [ 3954.9, 293.90000000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos34", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.455], "Focus": [17741.4], "XYStage": [ -1324.9, 545.6 ] }, "GridColumnIndex": 0 }, { "Label": "Pos35", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.445], "Focus": [17741.3], "XYStage": [ -2097, 576.6 ] }, "GridColumnIndex": 0 } ], "z-step_um": 10, "GridRow": 0, "BitDepth": 16, "Depth": 2, "Channels": 6, "Source": "Micro-Manager", "ChColors": [ -16711681, -256, -65281, -1, -1, -16776961 ], "MetadataVersion": 10, "KeepShutterOpenChannels": true, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536, 65536 ], "CustomIntervals_ms": [], "Height": 1200, "MicroManagerVersion": "1.4.23 20180219", "GridColumn": 0, "UUID": "14a13bf7-c944-4672-b873-fcfbbb7ea13d", "PixelType": "GRAY16", "Width": 1200, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\Yan\\20191218-YC113-S7S8-miseq", "Frames": 1, "Comment": "", "UserName": "Nikon", "PixelSize_um": 1.1, "ROI": [ 0, 0, 1200, 1200 ], "Date": "2019-12-18", "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC", "P-DAPI-high" ], "KeepShutterOpenSlices": true, "IJType": 1, "ComputerName": "ZadorConfocal2", "Positions": 36, "PositionIndex": 9, "CameraTimeout": 20000, "TimeFirst": false }, "640 AO-Name": "AnalogIO", "Vincent-D3-Command": "Close" }, "FrameKey-0-3-8": { "COM4-StopBits": "1", "528 AO-MaxVolts": "5.0000", "XLIGHT Excitation Wheel-State": "1", "470 Shutter-Name": "AnalogIO", "Core-Shutter": "640", "555 AO-Demo": "No", "XLIGHT Dichroic-State": "2", "XYStage-StepSize-Y": "0.1000", "ZPositionUm": 278.82, "XYStage-StepSize-X": "0.1000", "PRIME-FirmwareVersion": "20.28", "PRIME-ClearCycles": "2", "520-Name": "DA Shutter", "405 Shutter-Name": "AnalogIO", "PIEZO-StepSize": "0.0050", "PRIME-ClearMode": "Pre-Exposure", "470 AO-MinVolts": "0.0000", "405 AO-SupportsTriggering": "1", "CameraChannelIndex": 0, "XYStage-StartSpeed": "500.0000", "COM9-StopBits": "2", "405 AO-Description": "NI DAC", "COM4-DelayBetweenCharsMs": "0.0000", "445 AO-Block voltage": "false", "520 AO-TriggerInputLine": "/Dev1/PFI0", "555 AO-Description": "NI DAC", "520 AO-TriggerSampleRate": "100000", "COM4-Verbose": "1", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM4-Name": "COM4", "405 Shutter-Volts": "0.0000", "Vincent-D3-Port": "COM1", "PRIME-PP 4 MIN ADU AFFECTED": "200", "470 Shutter-MinVolts": "0.0000", "PRIME-PP 3 ENABLED": "Yes", "470 AO-Description": "NI DAC", "PRIME-Description": "PVCAM API device adapter", "520 Shutter-MaxVolts": "5.0000", "520 Shutter-Volts": "0.0000", "528 AO-Sequenceable": "1", "PRIME-Binning": "1x1", "555 Shutter-MaxVolts": "5.0000", "528 Shutter-TriggerSequenceLength": "1000", "405 Shutter-Block voltage": "false", "XLIGHT Spin motor-State": "1", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "Vincent-D3-Time to open (ms)": "35.0000", "Vincent-D3-Time to close (ms)": "35.0000", "445 AO-TriggerSampleRate": "100000", "640 Shutter-IOChannel": "Dev1/ao14", "555 AO-Sequenceable": "1", "520 AO-Name": "AnalogIO", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "470 AO-Block voltage": "false", "COM1-Verbose": "1", "COM9-AnswerTimeout": "10000.0000", "528 Shutter-Name": "AnalogIO", "PRIME-Trigger Timeout (secs)": "10", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "NextFrame": 0, "470 AO-TriggerSequenceLength": "1000", "PRIME-TransposeXY": "0", "445 AO-TriggerSequenceLength": "1000", "COM1-Name": "COM1", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "COM9-DataBits": "8", "COM1-AnswerTimeout": "500.0000", "Camera": "", "TE2000-Version": "V2.50-V2.50-V2.10", "528 Shutter-Volts": "0.0000", "XLIGHT Hub-Name": "XLIGHT Hub", "PIEZO-Description": "Ludl stage driver adapter", "PositionName": "Pos9", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSampleRate": "100000", "PRIME-FanSpeedSetpoint": "High", "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "405 AO-TriggerSampleRate": "100000", "470 Shutter-Block voltage": "false", "470-State": "0", "COM3-Parity": "None", "405 AO-Volts": "0.0000", "PRIME-TransposeMirrorY": "0", "PRIME-ChipName": "GS144BSI", "470 AO-Volts": "0.0000", "PRIME-TransposeMirrorX": "0", "445 Shutter-SupportsTriggering": "0", "470 Shutter-SupportsTriggering": "0", "XLIGHT Hub-Description": "XLIGHT hub", "PRIME-PreampOffLimit": "10000", "470 AO-Sequenceable": "1", "528 Shutter-MaxVolts": "5.0000", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "Core-XYStage": "XYStage", "470 AO-TriggerSampleRate": "100000", "PRIME-Timing-PreTriggerDelayNs": "24672000.0000", "528-DA Device": "528 Shutter", "Focus-Description": "Nikon TE2000 focus adapter", "FilterBlock-State": "5", "520 AO-Demo": "No", "COM3-DelayBetweenCharsMs": "0.0000", "470 Shutter-Volts": "0.0000", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "PRIME-PP 2 THRESHOLD": "125", "PRIME-PP 4 THRESHOLD": "75", "XYStage-Acceleration": "4.0000", "470 Shutter-Demo": "No", "PRIME-Name": "PMPCIECam00", "640-DA Device": "640 Shutter", "405 AO-Block voltage": "false", "ElapsedTime-ms": 399951, "PRIME-Offset": "100", "445 AO-IOChannel": "Dev1/ao1", "640 AO-MaxVolts": "5.0000", "PositionIndex": 9, "PRIME-PP 1 ENABLED": "Yes", "COM1-BaudRate": "9600", "640-State": "1", "COM3-Verbose": "1", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "LudlController-TransmissionDelay": "0", "PRIME-FullWellCapacity": "62000", "PRIME-SMARTStreamingEnabled": "No", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "640 Shutter-Description": "NI DAC", "528-Description": "DA device that is used as a shutter", "COM4-Description": "Serial port driver (boost:asio)", "405 Shutter-Description": "NI DAC", "TE2000-Name": "TE2000", "528 Shutter-Description": "NI DAC", "COM9-Description": "Serial port driver (boost:asio)", "LudlController-Name": "LudlController", "445 Shutter-TriggerSequenceLength": "1000", "PRIME-SerialNumber": "A18B203008", "445 AO-Volts": "0.0000", "PRIME-X-dimension": "1200", "PRIME-ShutterCloseDelay": "0", "XLIGHT Disk slider-State": "2", "Position": "Default", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 AO-IOChannel": "Dev1/ao5", "520 AO-IOChannel": "Dev1/ao4", "528 AO-TriggerSequenceLength": "1000", "405 Shutter-MaxVolts": "5.0000", "470-DA Device": "470 Shutter", "PRIME-FTCapable": "No", "Core-ChannelGroup": "Channels", "555 AO-SupportsTriggering": "0", "PRIME-Y-dimension": "1200", "Frame": 0, "555 Shutter-Sequenceable": "1", "PRIME-Timing-ExposureTimeNs": "499998000.0000", "Core-ImageProcessor": "", "528-State": "0", "520 AO-Block voltage": "false", "XLIGHT Excitation Wheel-Label": "QUAD", "640 Shutter-Demo": "No", "COM3-StopBits": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "520 Shutter-IOChannel": "Dev1/ao11", "Width": 1200, "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-SupportsTriggering": "0", "640 AO-TriggerSampleRate": "100000", "520-Description": "DA device that is used as a shutter", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "405 Shutter-Sequenceable": "1", "405 Shutter-SupportsTriggering": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-TriggerSampleRate": "100000", "640 Shutter-MaxVolts": "5.0000", "555 AO-MaxVolts": "5.0000", "520 AO-TriggerSequenceLength": "1000", "COM3-BaudRate": "9600", "COM9-BaudRate": "9600", "Vincent-D3-Channel #": "Ch. #1", "PRIME-PMode": "Normal", "OpticalPath-Label": "Left(100)", "BitDepth": 16, "555 AO-TriggerSequenceLength": "1000", "445 Shutter-Description": "NI DAC", "405 AO-Sequenceable": "1", "PRIME-AcquisitionMethod": "Callbacks", "555 AO-Name": "AnalogIO", "PRIME-ShutterMode": "Pre-Exposure", "COM3-Handshaking": "Off", "445 AO-SupportsTriggering": "0", "ROI": "0-0-1200-1200", "COM9-Verbose": "1", "PRIME-Color": "OFF", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "PRIME-ShutterOpenDelay": "0", "COM1-Parity": "None", "Slice": 8, "SlicePosition": 30, "COM9-DelayBetweenCharsMs": "0.0000", "555-Name": "DA Shutter", "PIEZO-LudlSingleAxisName": "B", "XLIGHT Emission Wheel-Label": "725/40 (C)", "640 Shutter-Name": "AnalogIO", "640 AO-Sequenceable": "1", "520 Shutter-Demo": "No", "528 Shutter-Demo": "No", "470-Name": "DA Shutter", "520-DA Device": "520 Shutter", "XYStage-Description": "Ludl XY stage driver adapter", "445 Shutter-MinVolts": "0.0000", "528 AO-TriggerSampleRate": "100000", "405 AO-MinVolts": "0.0000", "405 Shutter-TriggerSequenceLength": "1000", "Height": 1200, "555 AO-Block voltage": "false", "640 Shutter-Volts": "5.0000", "PixelType": "GRAY16", "445 Shutter-Block voltage": "false", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "520 Shutter-TriggerSequenceLength": "1000", "COM9-Name": "COM9", "Core-SLM": "", "555 AO-Volts": "0.0000", "555 Shutter-Block voltage": "false", "XYStage-Speed": "1800.0000", "520 AO-MaxVolts": "5.0000", "528 Shutter-SupportsTriggering": "0", "COM1-Description": "Serial port driver (boost:asio)", "PRIME-MetadataEnabled": "No", "520 Shutter-MinVolts": "0.0000", "470 Shutter-Sequenceable": "1", "PRIME-PreampDelay": "0", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-MinVolts": "0.0000", "ChannelIndex": 3, "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Core-AutoShutter": "1", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "Exposure-ms": 520, "PRIME-PP 2 ENABLED": "Yes", "640 Shutter-TriggerSampleRate": "100000", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "470 Shutter-IOChannel": "Dev1/ao10", "405 AO-Demo": "No", "PixelSizeUm": 1.1, "470 AO-Demo": "No", "470 Shutter-MaxVolts": "5.0000", "Vincent-D3-Name": "Vincent-D3", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FileName": "img_000000000_Il-C_008.tif", "LudlController-Reset": "Operate", "PRIME-Exposure": "520.00", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "445-Name": "DA Shutter", "555 Shutter-Name": "AnalogIO", "520 Shutter-TriggerSampleRate": "100000", "445 Shutter-IOChannel": "Dev1/ao9", "PRIME-TransposeCorrection": "0", "XYStage-StepSize": "0.1000", "445-Description": "DA device that is used as a shutter", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 AO-IOChannel": "Dev1/ao7", "520 Shutter-Name": "AnalogIO", "COM3-DataBits": "8", "640 AO-Volts": "4.5000", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "555 Shutter-IOChannel": "Dev1/ao13", "WaitInterval": null, "520 Shutter-Sequenceable": "1", "YPositionUm": 31506.2, "445-State": "0", "445-DA Device": "445 Shutter", "PRIME-CircularBufferAutoSize": "ON", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "555 AO-TriggerSampleRate": "100000", "445 Shutter-Volts": "0.0000", "XLIGHT Hub-Port": "COM3", "Core-AutoFocus": "", "COM4-Handshaking": "Off", "528 Shutter-MinVolts": "0.0000", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "445 Shutter-Name": "AnalogIO", "528 Shutter-Sequenceable": "1", "XYStage-TransposeMirrorX": "0", "XYStage-TransposeMirrorY": "0", "PRIME-ReadoutRate": "100MHz 16bit", "XYStage-Name": "Stage", "640 AO-SupportsTriggering": "0", "640-Name": "DA Shutter", "640 Shutter-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "640 AO-Block voltage": "false", "445 Shutter-Sequenceable": "1", "470 AO-MaxVolts": "5.0000", "COM4-BaudRate": "9600", "470-Description": "DA device that is used as a shutter", "520 Shutter-Description": "NI DAC", "PRIME-CCDTemperature": "-14.8500", "Time": "2019-12-18 21:37:07 -0500", "640 AO-TriggerSequenceLength": "1000", "470 AO-Name": "AnalogIO", "XLIGHT Disk slider-Label": "Disk Pos 40um", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "Binning": "1x1", "TE2000-ModelType": "1", "COM3-AnswerTimeout": "500.0000", "555 Shutter-SupportsTriggering": "0", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "TE2000-Port": "COM4", "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Description": "NI DAC", "COM4-AnswerTimeout": "500.0000", "COM1-DelayBetweenCharsMs": "0.0000", "FilterBlock-Label": "Confocal", "445 AO-Name": "AnalogIO", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-Timing-ReadoutTimeNs": "24672000.0000", "528 AO-Demo": "No", "520 AO-Sequenceable": "1", "555-Description": "DA device that is used as a shutter", "405-DA Device": "405 Shutter", "405 AO-TriggerSequenceLength": "1000", "640 AO-MinVolts": "0.0000", "640 Shutter-Block voltage": "false", "FrameIndex": 0, "PRIME-Port": "CMOS", "555 AO-MinVolts": "0.0000", "COM3-Description": "Serial port driver (boost:asio)", "528 AO-Description": "NI DAC", "555 AO-IOChannel": "Dev1/ao6", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "555 AO-TriggerInputLine": "/Dev1/PFI0", "PRIME-ActualInterval-ms": "390.5200", "LudlController-Port": "COM9", "445 Shutter-Demo": "No", "PRIME-Trigger-Expose Out-Mux": "1", "SliceIndex": 8, "PRIME-CCDTemperatureSetPoint": "-15.0000", "405 Shutter-MinVolts": "0.0000", "PRIME-ExposeOutMode": "First Row", "AxisPositions": null, "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Volts": "0.0000", "528-Name": "DA Shutter", "555-State": "0", "XLIGHT Dichroic-Label": "Di03-R635-t1", "445 AO-Sequenceable": "1", "405-Name": "DA Shutter", "405 AO-IOChannel": "Dev1/ao0", "Core-Camera": "PRIME", "Focus-Name": "Focus", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "405 Shutter-IOChannel": "Dev1/ao8", "640 AO-Demo": "No", "640 Shutter-SupportsTriggering": "0", "445 AO-Demo": "No", "555-DA Device": "555 Shutter", "470 Shutter-Description": "NI DAC", "528 AO-Volts": "0.0000", "COM1-DataBits": "8", "Vincent-D3-Address": "x", "520 AO-SupportsTriggering": "0", "405-Description": "DA device that is used as a shutter", "640 Shutter-MinVolts": "0.0000", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "405 AO-Name": "AnalogIO", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "640 AO-TriggerInputLine": "/Dev1/PFI0", "405 AO-TriggerInputLine": "/Dev1/PFI0", "640-Description": "DA device that is used as a shutter", "445 Shutter-TriggerSampleRate": "100000", "470 Shutter-TriggerSequenceLength": "1000", "COM1-StopBits": "1", "520 Shutter-SupportsTriggering": "0", "405-State": "0", "Core-Initialize": "1", "XLIGHT Emission Wheel-State": "3", "520 AO-Description": "NI DAC", "528 AO-TriggerInputLine": "/Dev1/PFI0", "445 Shutter-MaxVolts": "5.0000", "COM9-Handshaking": "Off", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "XLIGHT Spin motor-Label": "On (spinning)", "PRIME-PP 5 ENABLED": "No", "UUID": "703996d0-4138-4c77-914b-61ae99671a26", "PRIME-TriggerMode": "Internal Trigger", "COM1-Handshaking": "Off", "PRIME-Timing-PostTriggerDelayNs": "49344000.0000", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "Focus-Position": "17741.5000", "528 Shutter-Block voltage": "false", "Channel": "Il-C", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "555 Shutter-Demo": "No", "405 Shutter-Demo": "No", "528 AO-Name": "AnalogIO", "520-State": "0", "Core-Galvo": "", "405 Shutter-TriggerSampleRate": "100000", "Focus-FocusStepSize_(nm)": "100", "PRIME-PixelType": "16bit", "COM4-DataBits": "8", "470 AO-IOChannel": "Dev1/ao2", "520 AO-MinVolts": "0.0000", "COM9-Parity": "None", "PRIME-CircularBufferEnabled": "ON", "PRIME-PP 6 Reset": "No", "640 AO-Description": "NI DAC", "Source": "PRIME", "445 AO-MinVolts": "0.0000", "PIEZO-Name": "Stage", "445 AO-Description": "NI DAC", "PRIME-CircularBufferFrameCount": "46", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "520 AO-Volts": "0.0000", "528 Shutter-TriggerSampleRate": "100000", "XPositionUm": 19731.9, "OpticalPath-State": "4", "PRIME-PP 3 THRESHOLD": "80", "PRIME-PVCAM Version": "3.7.1", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Gain": "1-HDR", "640 Shutter-TriggerSequenceLength": "1000", "528 AO-SupportsTriggering": "0", "445 AO-TriggerInputLine": "/Dev1/PFI0", "OpticalPath-Name": "OpticalPath", "PRIME-PP 4 ENABLED": "Yes", "PRIME-PVCAM Adapter Version": "1.3.29", "COM3-Name": "COM3", "405 AO-MaxVolts": "5.0000", "520 Shutter-Block voltage": "false", "PRIME-Timing-ClearingTimeNs": "49344000.0000", "Core-Focus": "PIEZO", "528 AO-Block voltage": "false", "TE2000-Description": "Nikon TE2000 hub", "528 AO-MinVolts": "0.0000", "COM4-Parity": "None", "Summary": { "Slices": 11, "ChContrastMin": [ 0, 0, 0, 0, 0, 0 ], "PixelAspect": 1, "SlicesFirst": true, "Interval_ms": 0, "Time": "2019-12-18 21:36:42 -0500", "Prefix": "seq_4", "InitialPositionList": [ { "Label": "Pos0", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.21], "Focus": [17741.5], "XYStage": [ 44542.200000000004, 31636.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos1", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.22], "Focus": [17741.4], "XYStage": [ 43801.100000000006, 31637.9 ] }, "GridColumnIndex": 0 }, { "Label": "Pos2", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.5], "XYStage": [ 38561.3, 32040.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos3", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.185], "Focus": [17741.3], "XYStage": [ 37698.4, 32041.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos4", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.16], "Focus": [17741.5], "XYStage": [ 32375.4, 31758.9 ] }, "GridColumnIndex": 0 }, { "Label": "Pos5", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.17000000000002], "Focus": [17741.3], "XYStage": [ 31770.7, 31758.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos6", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.4], "XYStage": [ 26536.800000000003, 31068.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos7", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.205], "Focus": [17741.4], "XYStage": [ 25420.2, 31068.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos8", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.85], "Focus": [17741.3], "XYStage": [ 21059, 31508 ] }, "GridColumnIndex": 0 }, { "Label": "Pos9", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.82], "Focus": [17741.3], "XYStage": [ 19731.9, 31506.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos10", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.79], "Focus": [17741.4], "XYStage": [ 14528.5, 32007.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos11", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.83], "Focus": [17741.2], "XYStage": [ 13830.300000000001, 32009.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos12", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.79], "Focus": [17741.3], "XYStage": [ 8775.300000000001, 32017.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos13", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.815], "Focus": [17741.3], "XYStage": [ 8109.200000000001, 32008.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos14", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.805], "Focus": [17741.3], "XYStage": [ 3483.3, 31908.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos15", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.82], "Focus": [17741.2], "XYStage": [ 2686.8, 31903.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos16", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [211.48000000000002], "Focus": [17741.1], "XYStage": [ -1978.9, 31973.600000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos17", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [211.51500000000001], "Focus": [17741.1], "XYStage": [ -2957.8, 31982.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos18", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.195], "Focus": [17741.5], "XYStage": [ 47747.4, -576.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos19", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.23000000000002], "Focus": [17741.6], "XYStage": [ 47053.9, -576.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos20", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.205], "Focus": [17741.4], "XYStage": [ 41871.200000000004, -479.8 ] }, "GridColumnIndex": 0 }, { "Label": "Pos21", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.4], "XYStage": [ 40882.600000000006, -392 ] }, "GridColumnIndex": 0 }, { "Label": "Pos22", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.24], "Focus": [17741.4], "XYStage": [ 35905.6, -135.3 ] }, "GridColumnIndex": 0 }, { "Label": "Pos23", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.195], "Focus": [17741.5], "XYStage": [ 34990.9, -132.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos24", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.46], "Focus": [17741.4], "XYStage": [ 29678.100000000002, -958.1 ] }, "GridColumnIndex": 0 }, { "Label": "Pos25", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.44], "Focus": [17741.5], "XYStage": [ 28983.600000000002, -939.4000000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos26", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.475], "Focus": [17741.5], "XYStage": [ 23019.7, -122 ] }, "GridColumnIndex": 0 }, { "Label": "Pos27", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.44], "Focus": [17741.7], "XYStage": [ 22428, -116.80000000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos28", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.46], "Focus": [17741.7], "XYStage": [ 16364.900000000001, -181.10000000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos29", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.48000000000002], "Focus": [17741.5], "XYStage": [ 15712.1, -180 ] }, "GridColumnIndex": 0 }, { "Label": "Pos30", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.45000000000002], "Focus": [17741.7], "XYStage": [ 10346.1, 77.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos31", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.49], "Focus": [17741.4], "XYStage": [ 9755.5, 85.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos32", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.475], "Focus": [17741.5], "XYStage": [ 4775.5, 293.90000000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos33", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.445], "Focus": [17741.4], "XYStage": [ 3954.9, 293.90000000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos34", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.455], "Focus": [17741.4], "XYStage": [ -1324.9, 545.6 ] }, "GridColumnIndex": 0 }, { "Label": "Pos35", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.445], "Focus": [17741.3], "XYStage": [ -2097, 576.6 ] }, "GridColumnIndex": 0 } ], "z-step_um": 10, "GridRow": 0, "BitDepth": 16, "Depth": 2, "Channels": 6, "Source": "Micro-Manager", "ChColors": [ -16711681, -256, -65281, -1, -1, -16776961 ], "MetadataVersion": 10, "KeepShutterOpenChannels": true, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536, 65536 ], "CustomIntervals_ms": [], "Height": 1200, "MicroManagerVersion": "1.4.23 20180219", "GridColumn": 0, "UUID": "14a13bf7-c944-4672-b873-fcfbbb7ea13d", "PixelType": "GRAY16", "Width": 1200, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\Yan\\20191218-YC113-S7S8-miseq", "Frames": 1, "Comment": "", "UserName": "Nikon", "PixelSize_um": 1.1, "ROI": [ 0, 0, 1200, 1200 ], "Date": "2019-12-18", "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC", "P-DAPI-high" ], "KeepShutterOpenSlices": true, "IJType": 1, "ComputerName": "ZadorConfocal2", "Positions": 36, "PositionIndex": 9, "CameraTimeout": 20000, "TimeFirst": false }, "640 AO-Name": "AnalogIO", "Vincent-D3-Command": "Close" }, "FrameKey-0-3-9": { "COM4-StopBits": "1", "528 AO-MaxVolts": "5.0000", "XLIGHT Excitation Wheel-State": "1", "470 Shutter-Name": "AnalogIO", "Core-Shutter": "640", "555 AO-Demo": "No", "XLIGHT Dichroic-State": "2", "XYStage-StepSize-Y": "0.1000", "ZPositionUm": 288.82, "XYStage-StepSize-X": "0.1000", "PRIME-FirmwareVersion": "20.28", "PRIME-ClearCycles": "2", "520-Name": "DA Shutter", "405 Shutter-Name": "AnalogIO", "PIEZO-StepSize": "0.0050", "PRIME-ClearMode": "Pre-Exposure", "470 AO-MinVolts": "0.0000", "405 AO-SupportsTriggering": "1", "CameraChannelIndex": 0, "XYStage-StartSpeed": "500.0000", "COM9-StopBits": "2", "405 AO-Description": "NI DAC", "COM4-DelayBetweenCharsMs": "0.0000", "445 AO-Block voltage": "false", "520 AO-TriggerInputLine": "/Dev1/PFI0", "555 AO-Description": "NI DAC", "520 AO-TriggerSampleRate": "100000", "COM4-Verbose": "1", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM4-Name": "COM4", "405 Shutter-Volts": "0.0000", "Vincent-D3-Port": "COM1", "PRIME-PP 4 MIN ADU AFFECTED": "200", "470 Shutter-MinVolts": "0.0000", "PRIME-PP 3 ENABLED": "Yes", "470 AO-Description": "NI DAC", "PRIME-Description": "PVCAM API device adapter", "520 Shutter-MaxVolts": "5.0000", "520 Shutter-Volts": "0.0000", "528 AO-Sequenceable": "1", "PRIME-Binning": "1x1", "555 Shutter-MaxVolts": "5.0000", "528 Shutter-TriggerSequenceLength": "1000", "405 Shutter-Block voltage": "false", "XLIGHT Spin motor-State": "1", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "Vincent-D3-Time to open (ms)": "35.0000", "Vincent-D3-Time to close (ms)": "35.0000", "445 AO-TriggerSampleRate": "100000", "640 Shutter-IOChannel": "Dev1/ao14", "555 AO-Sequenceable": "1", "520 AO-Name": "AnalogIO", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "470 AO-Block voltage": "false", "COM1-Verbose": "1", "COM9-AnswerTimeout": "10000.0000", "528 Shutter-Name": "AnalogIO", "PRIME-Trigger Timeout (secs)": "10", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "NextFrame": 0, "470 AO-TriggerSequenceLength": "1000", "PRIME-TransposeXY": "0", "445 AO-TriggerSequenceLength": "1000", "COM1-Name": "COM1", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "COM9-DataBits": "8", "COM1-AnswerTimeout": "500.0000", "Camera": "", "TE2000-Version": "V2.50-V2.50-V2.10", "528 Shutter-Volts": "0.0000", "XLIGHT Hub-Name": "XLIGHT Hub", "PIEZO-Description": "Ludl stage driver adapter", "PositionName": "Pos9", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSampleRate": "100000", "PRIME-FanSpeedSetpoint": "High", "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "405 AO-TriggerSampleRate": "100000", "470 Shutter-Block voltage": "false", "470-State": "0", "COM3-Parity": "None", "405 AO-Volts": "0.0000", "PRIME-TransposeMirrorY": "0", "PRIME-ChipName": "GS144BSI", "470 AO-Volts": "0.0000", "PRIME-TransposeMirrorX": "0", "445 Shutter-SupportsTriggering": "0", "470 Shutter-SupportsTriggering": "0", "XLIGHT Hub-Description": "XLIGHT hub", "PRIME-PreampOffLimit": "10000", "470 AO-Sequenceable": "1", "528 Shutter-MaxVolts": "5.0000", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "Core-XYStage": "XYStage", "470 AO-TriggerSampleRate": "100000", "PRIME-Timing-PreTriggerDelayNs": "24672000.0000", "528-DA Device": "528 Shutter", "Focus-Description": "Nikon TE2000 focus adapter", "FilterBlock-State": "5", "520 AO-Demo": "No", "COM3-DelayBetweenCharsMs": "0.0000", "470 Shutter-Volts": "0.0000", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "PRIME-PP 2 THRESHOLD": "125", "PRIME-PP 4 THRESHOLD": "75", "XYStage-Acceleration": "4.0000", "470 Shutter-Demo": "No", "PRIME-Name": "PMPCIECam00", "640-DA Device": "640 Shutter", "405 AO-Block voltage": "false", "ElapsedTime-ms": 400755, "PRIME-Offset": "100", "445 AO-IOChannel": "Dev1/ao1", "640 AO-MaxVolts": "5.0000", "PositionIndex": 9, "PRIME-PP 1 ENABLED": "Yes", "COM1-BaudRate": "9600", "640-State": "1", "COM3-Verbose": "1", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "LudlController-TransmissionDelay": "0", "PRIME-FullWellCapacity": "62000", "PRIME-SMARTStreamingEnabled": "No", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "640 Shutter-Description": "NI DAC", "528-Description": "DA device that is used as a shutter", "COM4-Description": "Serial port driver (boost:asio)", "405 Shutter-Description": "NI DAC", "TE2000-Name": "TE2000", "528 Shutter-Description": "NI DAC", "COM9-Description": "Serial port driver (boost:asio)", "LudlController-Name": "LudlController", "445 Shutter-TriggerSequenceLength": "1000", "PRIME-SerialNumber": "A18B203008", "445 AO-Volts": "0.0000", "PRIME-X-dimension": "1200", "PRIME-ShutterCloseDelay": "0", "XLIGHT Disk slider-State": "2", "Position": "Default", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 AO-IOChannel": "Dev1/ao5", "520 AO-IOChannel": "Dev1/ao4", "528 AO-TriggerSequenceLength": "1000", "405 Shutter-MaxVolts": "5.0000", "470-DA Device": "470 Shutter", "PRIME-FTCapable": "No", "Core-ChannelGroup": "Channels", "555 AO-SupportsTriggering": "0", "PRIME-Y-dimension": "1200", "Frame": 0, "555 Shutter-Sequenceable": "1", "PRIME-Timing-ExposureTimeNs": "499998000.0000", "Core-ImageProcessor": "", "528-State": "0", "520 AO-Block voltage": "false", "XLIGHT Excitation Wheel-Label": "QUAD", "640 Shutter-Demo": "No", "COM3-StopBits": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "520 Shutter-IOChannel": "Dev1/ao11", "Width": 1200, "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-SupportsTriggering": "0", "640 AO-TriggerSampleRate": "100000", "520-Description": "DA device that is used as a shutter", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "405 Shutter-Sequenceable": "1", "405 Shutter-SupportsTriggering": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-TriggerSampleRate": "100000", "640 Shutter-MaxVolts": "5.0000", "555 AO-MaxVolts": "5.0000", "520 AO-TriggerSequenceLength": "1000", "COM3-BaudRate": "9600", "COM9-BaudRate": "9600", "Vincent-D3-Channel #": "Ch. #1", "PRIME-PMode": "Normal", "OpticalPath-Label": "Left(100)", "BitDepth": 16, "555 AO-TriggerSequenceLength": "1000", "445 Shutter-Description": "NI DAC", "405 AO-Sequenceable": "1", "PRIME-AcquisitionMethod": "Callbacks", "555 AO-Name": "AnalogIO", "PRIME-ShutterMode": "Pre-Exposure", "COM3-Handshaking": "Off", "445 AO-SupportsTriggering": "0", "ROI": "0-0-1200-1200", "COM9-Verbose": "1", "PRIME-Color": "OFF", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "PRIME-ShutterOpenDelay": "0", "COM1-Parity": "None", "Slice": 9, "SlicePosition": 40, "COM9-DelayBetweenCharsMs": "0.0000", "555-Name": "DA Shutter", "PIEZO-LudlSingleAxisName": "B", "XLIGHT Emission Wheel-Label": "725/40 (C)", "640 Shutter-Name": "AnalogIO", "640 AO-Sequenceable": "1", "520 Shutter-Demo": "No", "528 Shutter-Demo": "No", "470-Name": "DA Shutter", "520-DA Device": "520 Shutter", "XYStage-Description": "Ludl XY stage driver adapter", "445 Shutter-MinVolts": "0.0000", "528 AO-TriggerSampleRate": "100000", "405 AO-MinVolts": "0.0000", "405 Shutter-TriggerSequenceLength": "1000", "Height": 1200, "555 AO-Block voltage": "false", "640 Shutter-Volts": "5.0000", "PixelType": "GRAY16", "445 Shutter-Block voltage": "false", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "520 Shutter-TriggerSequenceLength": "1000", "COM9-Name": "COM9", "Core-SLM": "", "555 AO-Volts": "0.0000", "555 Shutter-Block voltage": "false", "XYStage-Speed": "1800.0000", "520 AO-MaxVolts": "5.0000", "528 Shutter-SupportsTriggering": "0", "COM1-Description": "Serial port driver (boost:asio)", "PRIME-MetadataEnabled": "No", "520 Shutter-MinVolts": "0.0000", "470 Shutter-Sequenceable": "1", "PRIME-PreampDelay": "0", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-MinVolts": "0.0000", "ChannelIndex": 3, "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Core-AutoShutter": "1", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "Exposure-ms": 520, "PRIME-PP 2 ENABLED": "Yes", "640 Shutter-TriggerSampleRate": "100000", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "470 Shutter-IOChannel": "Dev1/ao10", "405 AO-Demo": "No", "PixelSizeUm": 1.1, "470 AO-Demo": "No", "470 Shutter-MaxVolts": "5.0000", "Vincent-D3-Name": "Vincent-D3", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FileName": "img_000000000_Il-C_009.tif", "LudlController-Reset": "Operate", "PRIME-Exposure": "520.00", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "445-Name": "DA Shutter", "555 Shutter-Name": "AnalogIO", "520 Shutter-TriggerSampleRate": "100000", "445 Shutter-IOChannel": "Dev1/ao9", "PRIME-TransposeCorrection": "0", "XYStage-StepSize": "0.1000", "445-Description": "DA device that is used as a shutter", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 AO-IOChannel": "Dev1/ao7", "520 Shutter-Name": "AnalogIO", "COM3-DataBits": "8", "640 AO-Volts": "4.5000", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "555 Shutter-IOChannel": "Dev1/ao13", "WaitInterval": null, "520 Shutter-Sequenceable": "1", "YPositionUm": 31506.2, "445-State": "0", "445-DA Device": "445 Shutter", "PRIME-CircularBufferAutoSize": "ON", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "555 AO-TriggerSampleRate": "100000", "445 Shutter-Volts": "0.0000", "XLIGHT Hub-Port": "COM3", "Core-AutoFocus": "", "COM4-Handshaking": "Off", "528 Shutter-MinVolts": "0.0000", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "445 Shutter-Name": "AnalogIO", "528 Shutter-Sequenceable": "1", "XYStage-TransposeMirrorX": "0", "XYStage-TransposeMirrorY": "0", "PRIME-ReadoutRate": "100MHz 16bit", "XYStage-Name": "Stage", "640 AO-SupportsTriggering": "0", "640-Name": "DA Shutter", "640 Shutter-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "640 AO-Block voltage": "false", "445 Shutter-Sequenceable": "1", "470 AO-MaxVolts": "5.0000", "COM4-BaudRate": "9600", "470-Description": "DA device that is used as a shutter", "520 Shutter-Description": "NI DAC", "PRIME-CCDTemperature": "-14.8500", "Time": "2019-12-18 21:37:08 -0500", "640 AO-TriggerSequenceLength": "1000", "470 AO-Name": "AnalogIO", "XLIGHT Disk slider-Label": "Disk Pos 40um", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "Binning": "1x1", "TE2000-ModelType": "1", "COM3-AnswerTimeout": "500.0000", "555 Shutter-SupportsTriggering": "0", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "TE2000-Port": "COM4", "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Description": "NI DAC", "COM4-AnswerTimeout": "500.0000", "COM1-DelayBetweenCharsMs": "0.0000", "FilterBlock-Label": "Confocal", "445 AO-Name": "AnalogIO", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-Timing-ReadoutTimeNs": "24672000.0000", "528 AO-Demo": "No", "520 AO-Sequenceable": "1", "555-Description": "DA device that is used as a shutter", "405-DA Device": "405 Shutter", "405 AO-TriggerSequenceLength": "1000", "640 AO-MinVolts": "0.0000", "640 Shutter-Block voltage": "false", "FrameIndex": 0, "PRIME-Port": "CMOS", "555 AO-MinVolts": "0.0000", "COM3-Description": "Serial port driver (boost:asio)", "528 AO-Description": "NI DAC", "555 AO-IOChannel": "Dev1/ao6", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "555 AO-TriggerInputLine": "/Dev1/PFI0", "PRIME-ActualInterval-ms": "390.5200", "LudlController-Port": "COM9", "445 Shutter-Demo": "No", "PRIME-Trigger-Expose Out-Mux": "1", "SliceIndex": 9, "PRIME-CCDTemperatureSetPoint": "-15.0000", "405 Shutter-MinVolts": "0.0000", "PRIME-ExposeOutMode": "First Row", "AxisPositions": null, "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Volts": "0.0000", "528-Name": "DA Shutter", "555-State": "0", "XLIGHT Dichroic-Label": "Di03-R635-t1", "445 AO-Sequenceable": "1", "405-Name": "DA Shutter", "405 AO-IOChannel": "Dev1/ao0", "Core-Camera": "PRIME", "Focus-Name": "Focus", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "405 Shutter-IOChannel": "Dev1/ao8", "640 AO-Demo": "No", "640 Shutter-SupportsTriggering": "0", "445 AO-Demo": "No", "555-DA Device": "555 Shutter", "470 Shutter-Description": "NI DAC", "528 AO-Volts": "0.0000", "COM1-DataBits": "8", "Vincent-D3-Address": "x", "520 AO-SupportsTriggering": "0", "405-Description": "DA device that is used as a shutter", "640 Shutter-MinVolts": "0.0000", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "405 AO-Name": "AnalogIO", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "640 AO-TriggerInputLine": "/Dev1/PFI0", "405 AO-TriggerInputLine": "/Dev1/PFI0", "640-Description": "DA device that is used as a shutter", "445 Shutter-TriggerSampleRate": "100000", "470 Shutter-TriggerSequenceLength": "1000", "COM1-StopBits": "1", "520 Shutter-SupportsTriggering": "0", "405-State": "0", "Core-Initialize": "1", "XLIGHT Emission Wheel-State": "3", "520 AO-Description": "NI DAC", "528 AO-TriggerInputLine": "/Dev1/PFI0", "445 Shutter-MaxVolts": "5.0000", "COM9-Handshaking": "Off", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "XLIGHT Spin motor-Label": "On (spinning)", "PRIME-PP 5 ENABLED": "No", "UUID": "32378be0-47b1-467e-b203-874787c445ad", "PRIME-TriggerMode": "Internal Trigger", "COM1-Handshaking": "Off", "PRIME-Timing-PostTriggerDelayNs": "49344000.0000", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "Focus-Position": "17741.5000", "528 Shutter-Block voltage": "false", "Channel": "Il-C", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "555 Shutter-Demo": "No", "405 Shutter-Demo": "No", "528 AO-Name": "AnalogIO", "520-State": "0", "Core-Galvo": "", "405 Shutter-TriggerSampleRate": "100000", "Focus-FocusStepSize_(nm)": "100", "PRIME-PixelType": "16bit", "COM4-DataBits": "8", "470 AO-IOChannel": "Dev1/ao2", "520 AO-MinVolts": "0.0000", "COM9-Parity": "None", "PRIME-CircularBufferEnabled": "ON", "PRIME-PP 6 Reset": "No", "640 AO-Description": "NI DAC", "Source": "PRIME", "445 AO-MinVolts": "0.0000", "PIEZO-Name": "Stage", "445 AO-Description": "NI DAC", "PRIME-CircularBufferFrameCount": "46", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "520 AO-Volts": "0.0000", "528 Shutter-TriggerSampleRate": "100000", "XPositionUm": 19731.9, "OpticalPath-State": "4", "PRIME-PP 3 THRESHOLD": "80", "PRIME-PVCAM Version": "3.7.1", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Gain": "1-HDR", "640 Shutter-TriggerSequenceLength": "1000", "528 AO-SupportsTriggering": "0", "445 AO-TriggerInputLine": "/Dev1/PFI0", "OpticalPath-Name": "OpticalPath", "PRIME-PP 4 ENABLED": "Yes", "PRIME-PVCAM Adapter Version": "1.3.29", "COM3-Name": "COM3", "405 AO-MaxVolts": "5.0000", "520 Shutter-Block voltage": "false", "PRIME-Timing-ClearingTimeNs": "49344000.0000", "Core-Focus": "PIEZO", "528 AO-Block voltage": "false", "TE2000-Description": "Nikon TE2000 hub", "528 AO-MinVolts": "0.0000", "COM4-Parity": "None", "Summary": { "Slices": 11, "ChContrastMin": [ 0, 0, 0, 0, 0, 0 ], "PixelAspect": 1, "SlicesFirst": true, "Interval_ms": 0, "Time": "2019-12-18 21:36:42 -0500", "Prefix": "seq_4", "InitialPositionList": [ { "Label": "Pos0", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.21], "Focus": [17741.5], "XYStage": [ 44542.200000000004, 31636.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos1", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.22], "Focus": [17741.4], "XYStage": [ 43801.100000000006, 31637.9 ] }, "GridColumnIndex": 0 }, { "Label": "Pos2", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.5], "XYStage": [ 38561.3, 32040.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos3", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.185], "Focus": [17741.3], "XYStage": [ 37698.4, 32041.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos4", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.16], "Focus": [17741.5], "XYStage": [ 32375.4, 31758.9 ] }, "GridColumnIndex": 0 }, { "Label": "Pos5", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.17000000000002], "Focus": [17741.3], "XYStage": [ 31770.7, 31758.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos6", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.4], "XYStage": [ 26536.800000000003, 31068.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos7", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.205], "Focus": [17741.4], "XYStage": [ 25420.2, 31068.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos8", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.85], "Focus": [17741.3], "XYStage": [ 21059, 31508 ] }, "GridColumnIndex": 0 }, { "Label": "Pos9", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.82], "Focus": [17741.3], "XYStage": [ 19731.9, 31506.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos10", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.79], "Focus": [17741.4], "XYStage": [ 14528.5, 32007.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos11", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.83], "Focus": [17741.2], "XYStage": [ 13830.300000000001, 32009.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos12", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.79], "Focus": [17741.3], "XYStage": [ 8775.300000000001, 32017.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos13", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.815], "Focus": [17741.3], "XYStage": [ 8109.200000000001, 32008.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos14", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.805], "Focus": [17741.3], "XYStage": [ 3483.3, 31908.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos15", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.82], "Focus": [17741.2], "XYStage": [ 2686.8, 31903.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos16", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [211.48000000000002], "Focus": [17741.1], "XYStage": [ -1978.9, 31973.600000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos17", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [211.51500000000001], "Focus": [17741.1], "XYStage": [ -2957.8, 31982.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos18", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.195], "Focus": [17741.5], "XYStage": [ 47747.4, -576.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos19", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.23000000000002], "Focus": [17741.6], "XYStage": [ 47053.9, -576.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos20", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.205], "Focus": [17741.4], "XYStage": [ 41871.200000000004, -479.8 ] }, "GridColumnIndex": 0 }, { "Label": "Pos21", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.4], "XYStage": [ 40882.600000000006, -392 ] }, "GridColumnIndex": 0 }, { "Label": "Pos22", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.24], "Focus": [17741.4], "XYStage": [ 35905.6, -135.3 ] }, "GridColumnIndex": 0 }, { "Label": "Pos23", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.195], "Focus": [17741.5], "XYStage": [ 34990.9, -132.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos24", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.46], "Focus": [17741.4], "XYStage": [ 29678.100000000002, -958.1 ] }, "GridColumnIndex": 0 }, { "Label": "Pos25", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.44], "Focus": [17741.5], "XYStage": [ 28983.600000000002, -939.4000000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos26", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.475], "Focus": [17741.5], "XYStage": [ 23019.7, -122 ] }, "GridColumnIndex": 0 }, { "Label": "Pos27", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.44], "Focus": [17741.7], "XYStage": [ 22428, -116.80000000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos28", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.46], "Focus": [17741.7], "XYStage": [ 16364.900000000001, -181.10000000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos29", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.48000000000002], "Focus": [17741.5], "XYStage": [ 15712.1, -180 ] }, "GridColumnIndex": 0 }, { "Label": "Pos30", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.45000000000002], "Focus": [17741.7], "XYStage": [ 10346.1, 77.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos31", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.49], "Focus": [17741.4], "XYStage": [ 9755.5, 85.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos32", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.475], "Focus": [17741.5], "XYStage": [ 4775.5, 293.90000000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos33", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.445], "Focus": [17741.4], "XYStage": [ 3954.9, 293.90000000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos34", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.455], "Focus": [17741.4], "XYStage": [ -1324.9, 545.6 ] }, "GridColumnIndex": 0 }, { "Label": "Pos35", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.445], "Focus": [17741.3], "XYStage": [ -2097, 576.6 ] }, "GridColumnIndex": 0 } ], "z-step_um": 10, "GridRow": 0, "BitDepth": 16, "Depth": 2, "Channels": 6, "Source": "Micro-Manager", "ChColors": [ -16711681, -256, -65281, -1, -1, -16776961 ], "MetadataVersion": 10, "KeepShutterOpenChannels": true, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536, 65536 ], "CustomIntervals_ms": [], "Height": 1200, "MicroManagerVersion": "1.4.23 20180219", "GridColumn": 0, "UUID": "14a13bf7-c944-4672-b873-fcfbbb7ea13d", "PixelType": "GRAY16", "Width": 1200, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\Yan\\20191218-YC113-S7S8-miseq", "Frames": 1, "Comment": "", "UserName": "Nikon", "PixelSize_um": 1.1, "ROI": [ 0, 0, 1200, 1200 ], "Date": "2019-12-18", "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC", "P-DAPI-high" ], "KeepShutterOpenSlices": true, "IJType": 1, "ComputerName": "ZadorConfocal2", "Positions": 36, "PositionIndex": 9, "CameraTimeout": 20000, "TimeFirst": false }, "640 AO-Name": "AnalogIO", "Vincent-D3-Command": "Close" }, "FrameKey-0-3-10": { "COM4-StopBits": "1", "528 AO-MaxVolts": "5.0000", "XLIGHT Excitation Wheel-State": "1", "470 Shutter-Name": "AnalogIO", "Core-Shutter": "640", "555 AO-Demo": "No", "XLIGHT Dichroic-State": "2", "XYStage-StepSize-Y": "0.1000", "ZPositionUm": 298.82, "XYStage-StepSize-X": "0.1000", "PRIME-FirmwareVersion": "20.28", "PRIME-ClearCycles": "2", "520-Name": "DA Shutter", "405 Shutter-Name": "AnalogIO", "PIEZO-StepSize": "0.0050", "PRIME-ClearMode": "Pre-Exposure", "470 AO-MinVolts": "0.0000", "405 AO-SupportsTriggering": "1", "CameraChannelIndex": 0, "XYStage-StartSpeed": "500.0000", "COM9-StopBits": "2", "405 AO-Description": "NI DAC", "COM4-DelayBetweenCharsMs": "0.0000", "445 AO-Block voltage": "false", "520 AO-TriggerInputLine": "/Dev1/PFI0", "555 AO-Description": "NI DAC", "520 AO-TriggerSampleRate": "100000", "COM4-Verbose": "1", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM4-Name": "COM4", "405 Shutter-Volts": "0.0000", "Vincent-D3-Port": "COM1", "PRIME-PP 4 MIN ADU AFFECTED": "200", "470 Shutter-MinVolts": "0.0000", "PRIME-PP 3 ENABLED": "Yes", "470 AO-Description": "NI DAC", "PRIME-Description": "PVCAM API device adapter", "520 Shutter-MaxVolts": "5.0000", "520 Shutter-Volts": "0.0000", "528 AO-Sequenceable": "1", "PRIME-Binning": "1x1", "555 Shutter-MaxVolts": "5.0000", "528 Shutter-TriggerSequenceLength": "1000", "405 Shutter-Block voltage": "false", "XLIGHT Spin motor-State": "1", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "Vincent-D3-Time to open (ms)": "35.0000", "Vincent-D3-Time to close (ms)": "35.0000", "445 AO-TriggerSampleRate": "100000", "640 Shutter-IOChannel": "Dev1/ao14", "555 AO-Sequenceable": "1", "520 AO-Name": "AnalogIO", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "470 AO-Block voltage": "false", "COM1-Verbose": "1", "COM9-AnswerTimeout": "10000.0000", "528 Shutter-Name": "AnalogIO", "PRIME-Trigger Timeout (secs)": "10", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "NextFrame": 0, "470 AO-TriggerSequenceLength": "1000", "PRIME-TransposeXY": "0", "445 AO-TriggerSequenceLength": "1000", "COM1-Name": "COM1", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "COM9-DataBits": "8", "COM1-AnswerTimeout": "500.0000", "Camera": "", "TE2000-Version": "V2.50-V2.50-V2.10", "528 Shutter-Volts": "0.0000", "XLIGHT Hub-Name": "XLIGHT Hub", "PIEZO-Description": "Ludl stage driver adapter", "PositionName": "Pos9", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSampleRate": "100000", "PRIME-FanSpeedSetpoint": "High", "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "405 AO-TriggerSampleRate": "100000", "470 Shutter-Block voltage": "false", "470-State": "0", "COM3-Parity": "None", "405 AO-Volts": "0.0000", "PRIME-TransposeMirrorY": "0", "PRIME-ChipName": "GS144BSI", "470 AO-Volts": "0.0000", "PRIME-TransposeMirrorX": "0", "445 Shutter-SupportsTriggering": "0", "470 Shutter-SupportsTriggering": "0", "XLIGHT Hub-Description": "XLIGHT hub", "PRIME-PreampOffLimit": "10000", "470 AO-Sequenceable": "1", "528 Shutter-MaxVolts": "5.0000", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "Core-XYStage": "XYStage", "470 AO-TriggerSampleRate": "100000", "PRIME-Timing-PreTriggerDelayNs": "24672000.0000", "528-DA Device": "528 Shutter", "Focus-Description": "Nikon TE2000 focus adapter", "FilterBlock-State": "5", "520 AO-Demo": "No", "COM3-DelayBetweenCharsMs": "0.0000", "470 Shutter-Volts": "0.0000", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "PRIME-PP 2 THRESHOLD": "125", "PRIME-PP 4 THRESHOLD": "75", "XYStage-Acceleration": "4.0000", "470 Shutter-Demo": "No", "PRIME-Name": "PMPCIECam00", "640-DA Device": "640 Shutter", "405 AO-Block voltage": "false", "ElapsedTime-ms": 401569, "PRIME-Offset": "100", "445 AO-IOChannel": "Dev1/ao1", "640 AO-MaxVolts": "5.0000", "PositionIndex": 9, "PRIME-PP 1 ENABLED": "Yes", "COM1-BaudRate": "9600", "640-State": "0", "COM3-Verbose": "1", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "LudlController-TransmissionDelay": "0", "PRIME-FullWellCapacity": "62000", "PRIME-SMARTStreamingEnabled": "No", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "640 Shutter-Description": "NI DAC", "528-Description": "DA device that is used as a shutter", "COM4-Description": "Serial port driver (boost:asio)", "405 Shutter-Description": "NI DAC", "TE2000-Name": "TE2000", "528 Shutter-Description": "NI DAC", "COM9-Description": "Serial port driver (boost:asio)", "LudlController-Name": "LudlController", "445 Shutter-TriggerSequenceLength": "1000", "PRIME-SerialNumber": "A18B203008", "445 AO-Volts": "0.0000", "PRIME-X-dimension": "1200", "PRIME-ShutterCloseDelay": "0", "XLIGHT Disk slider-State": "2", "Position": "Default", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 AO-IOChannel": "Dev1/ao5", "520 AO-IOChannel": "Dev1/ao4", "528 AO-TriggerSequenceLength": "1000", "405 Shutter-MaxVolts": "5.0000", "470-DA Device": "470 Shutter", "PRIME-FTCapable": "No", "Core-ChannelGroup": "Channels", "555 AO-SupportsTriggering": "0", "PRIME-Y-dimension": "1200", "Frame": 0, "555 Shutter-Sequenceable": "1", "PRIME-Timing-ExposureTimeNs": "499998000.0000", "Core-ImageProcessor": "", "528-State": "0", "520 AO-Block voltage": "false", "XLIGHT Excitation Wheel-Label": "QUAD", "640 Shutter-Demo": "No", "COM3-StopBits": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "520 Shutter-IOChannel": "Dev1/ao11", "Width": 1200, "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-SupportsTriggering": "0", "640 AO-TriggerSampleRate": "100000", "520-Description": "DA device that is used as a shutter", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "405 Shutter-Sequenceable": "1", "405 Shutter-SupportsTriggering": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-TriggerSampleRate": "100000", "640 Shutter-MaxVolts": "5.0000", "555 AO-MaxVolts": "5.0000", "520 AO-TriggerSequenceLength": "1000", "COM3-BaudRate": "9600", "COM9-BaudRate": "9600", "Vincent-D3-Channel #": "Ch. #1", "PRIME-PMode": "Normal", "OpticalPath-Label": "Left(100)", "BitDepth": 16, "555 AO-TriggerSequenceLength": "1000", "445 Shutter-Description": "NI DAC", "405 AO-Sequenceable": "1", "PRIME-AcquisitionMethod": "Callbacks", "555 AO-Name": "AnalogIO", "PRIME-ShutterMode": "Pre-Exposure", "COM3-Handshaking": "Off", "445 AO-SupportsTriggering": "0", "ROI": "0-0-1200-1200", "COM9-Verbose": "1", "PRIME-Color": "OFF", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "PRIME-ShutterOpenDelay": "0", "COM1-Parity": "None", "Slice": 10, "SlicePosition": 50, "COM9-DelayBetweenCharsMs": "0.0000", "555-Name": "DA Shutter", "PIEZO-LudlSingleAxisName": "B", "XLIGHT Emission Wheel-Label": "725/40 (C)", "640 Shutter-Name": "AnalogIO", "640 AO-Sequenceable": "1", "520 Shutter-Demo": "No", "528 Shutter-Demo": "No", "470-Name": "DA Shutter", "520-DA Device": "520 Shutter", "XYStage-Description": "Ludl XY stage driver adapter", "445 Shutter-MinVolts": "0.0000", "528 AO-TriggerSampleRate": "100000", "405 AO-MinVolts": "0.0000", "405 Shutter-TriggerSequenceLength": "1000", "Height": 1200, "555 AO-Block voltage": "false", "640 Shutter-Volts": "5.0000", "PixelType": "GRAY16", "445 Shutter-Block voltage": "false", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "520 Shutter-TriggerSequenceLength": "1000", "COM9-Name": "COM9", "Core-SLM": "", "555 AO-Volts": "0.0000", "555 Shutter-Block voltage": "false", "XYStage-Speed": "1800.0000", "520 AO-MaxVolts": "5.0000", "528 Shutter-SupportsTriggering": "0", "COM1-Description": "Serial port driver (boost:asio)", "PRIME-MetadataEnabled": "No", "520 Shutter-MinVolts": "0.0000", "470 Shutter-Sequenceable": "1", "PRIME-PreampDelay": "0", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-MinVolts": "0.0000", "ChannelIndex": 3, "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Core-AutoShutter": "1", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "Exposure-ms": 520, "PRIME-PP 2 ENABLED": "Yes", "640 Shutter-TriggerSampleRate": "100000", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "470 Shutter-IOChannel": "Dev1/ao10", "405 AO-Demo": "No", "PixelSizeUm": 1.1, "470 AO-Demo": "No", "470 Shutter-MaxVolts": "5.0000", "Vincent-D3-Name": "Vincent-D3", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FileName": "img_000000000_Il-C_010.tif", "LudlController-Reset": "Operate", "PRIME-Exposure": "520.00", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "445-Name": "DA Shutter", "555 Shutter-Name": "AnalogIO", "520 Shutter-TriggerSampleRate": "100000", "445 Shutter-IOChannel": "Dev1/ao9", "PRIME-TransposeCorrection": "0", "XYStage-StepSize": "0.1000", "445-Description": "DA device that is used as a shutter", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 AO-IOChannel": "Dev1/ao7", "520 Shutter-Name": "AnalogIO", "COM3-DataBits": "8", "640 AO-Volts": "4.5000", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "555 Shutter-IOChannel": "Dev1/ao13", "WaitInterval": null, "520 Shutter-Sequenceable": "1", "YPositionUm": 31506.2, "445-State": "0", "445-DA Device": "445 Shutter", "PRIME-CircularBufferAutoSize": "ON", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "555 AO-TriggerSampleRate": "100000", "445 Shutter-Volts": "0.0000", "XLIGHT Hub-Port": "COM3", "Core-AutoFocus": "", "COM4-Handshaking": "Off", "528 Shutter-MinVolts": "0.0000", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "445 Shutter-Name": "AnalogIO", "528 Shutter-Sequenceable": "1", "XYStage-TransposeMirrorX": "0", "XYStage-TransposeMirrorY": "0", "PRIME-ReadoutRate": "100MHz 16bit", "XYStage-Name": "Stage", "640 AO-SupportsTriggering": "0", "640-Name": "DA Shutter", "640 Shutter-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "640 AO-Block voltage": "false", "445 Shutter-Sequenceable": "1", "470 AO-MaxVolts": "5.0000", "COM4-BaudRate": "9600", "470-Description": "DA device that is used as a shutter", "520 Shutter-Description": "NI DAC", "PRIME-CCDTemperature": "-14.8500", "Time": "2019-12-18 21:37:09 -0500", "640 AO-TriggerSequenceLength": "1000", "470 AO-Name": "AnalogIO", "XLIGHT Disk slider-Label": "Disk Pos 40um", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "Binning": "1x1", "TE2000-ModelType": "1", "COM3-AnswerTimeout": "500.0000", "555 Shutter-SupportsTriggering": "0", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "TE2000-Port": "COM4", "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Description": "NI DAC", "COM4-AnswerTimeout": "500.0000", "COM1-DelayBetweenCharsMs": "0.0000", "FilterBlock-Label": "Confocal", "445 AO-Name": "AnalogIO", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-Timing-ReadoutTimeNs": "24672000.0000", "528 AO-Demo": "No", "520 AO-Sequenceable": "1", "555-Description": "DA device that is used as a shutter", "405-DA Device": "405 Shutter", "405 AO-TriggerSequenceLength": "1000", "640 AO-MinVolts": "0.0000", "640 Shutter-Block voltage": "false", "FrameIndex": 0, "PRIME-Port": "CMOS", "555 AO-MinVolts": "0.0000", "COM3-Description": "Serial port driver (boost:asio)", "528 AO-Description": "NI DAC", "555 AO-IOChannel": "Dev1/ao6", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "555 AO-TriggerInputLine": "/Dev1/PFI0", "PRIME-ActualInterval-ms": "390.5200", "LudlController-Port": "COM9", "445 Shutter-Demo": "No", "PRIME-Trigger-Expose Out-Mux": "1", "SliceIndex": 10, "PRIME-CCDTemperatureSetPoint": "-15.0000", "405 Shutter-MinVolts": "0.0000", "PRIME-ExposeOutMode": "First Row", "AxisPositions": null, "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Volts": "0.0000", "528-Name": "DA Shutter", "555-State": "0", "XLIGHT Dichroic-Label": "Di03-R635-t1", "445 AO-Sequenceable": "1", "405-Name": "DA Shutter", "405 AO-IOChannel": "Dev1/ao0", "Core-Camera": "PRIME", "Focus-Name": "Focus", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "405 Shutter-IOChannel": "Dev1/ao8", "640 AO-Demo": "No", "640 Shutter-SupportsTriggering": "0", "445 AO-Demo": "No", "555-DA Device": "555 Shutter", "470 Shutter-Description": "NI DAC", "528 AO-Volts": "0.0000", "COM1-DataBits": "8", "Vincent-D3-Address": "x", "520 AO-SupportsTriggering": "0", "405-Description": "DA device that is used as a shutter", "640 Shutter-MinVolts": "0.0000", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "405 AO-Name": "AnalogIO", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "640 AO-TriggerInputLine": "/Dev1/PFI0", "405 AO-TriggerInputLine": "/Dev1/PFI0", "640-Description": "DA device that is used as a shutter", "445 Shutter-TriggerSampleRate": "100000", "470 Shutter-TriggerSequenceLength": "1000", "COM1-StopBits": "1", "520 Shutter-SupportsTriggering": "0", "405-State": "0", "Core-Initialize": "1", "XLIGHT Emission Wheel-State": "3", "520 AO-Description": "NI DAC", "528 AO-TriggerInputLine": "/Dev1/PFI0", "445 Shutter-MaxVolts": "5.0000", "COM9-Handshaking": "Off", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "XLIGHT Spin motor-Label": "On (spinning)", "PRIME-PP 5 ENABLED": "No", "UUID": "dc5a9814-6ad9-4cd1-a233-7f43f2c7f4b4", "PRIME-TriggerMode": "Internal Trigger", "COM1-Handshaking": "Off", "PRIME-Timing-PostTriggerDelayNs": "49344000.0000", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "Focus-Position": "17741.5000", "528 Shutter-Block voltage": "false", "Channel": "Il-C", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "555 Shutter-Demo": "No", "405 Shutter-Demo": "No", "528 AO-Name": "AnalogIO", "520-State": "0", "Core-Galvo": "", "405 Shutter-TriggerSampleRate": "100000", "Focus-FocusStepSize_(nm)": "100", "PRIME-PixelType": "16bit", "COM4-DataBits": "8", "470 AO-IOChannel": "Dev1/ao2", "520 AO-MinVolts": "0.0000", "COM9-Parity": "None", "PRIME-CircularBufferEnabled": "ON", "PRIME-PP 6 Reset": "No", "640 AO-Description": "NI DAC", "Source": "PRIME", "445 AO-MinVolts": "0.0000", "PIEZO-Name": "Stage", "445 AO-Description": "NI DAC", "PRIME-CircularBufferFrameCount": "46", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "520 AO-Volts": "0.0000", "528 Shutter-TriggerSampleRate": "100000", "XPositionUm": 19731.9, "OpticalPath-State": "4", "PRIME-PP 3 THRESHOLD": "80", "PRIME-PVCAM Version": "3.7.1", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Gain": "1-HDR", "640 Shutter-TriggerSequenceLength": "1000", "528 AO-SupportsTriggering": "0", "445 AO-TriggerInputLine": "/Dev1/PFI0", "OpticalPath-Name": "OpticalPath", "PRIME-PP 4 ENABLED": "Yes", "PRIME-PVCAM Adapter Version": "1.3.29", "COM3-Name": "COM3", "405 AO-MaxVolts": "5.0000", "520 Shutter-Block voltage": "false", "PRIME-Timing-ClearingTimeNs": "49344000.0000", "Core-Focus": "PIEZO", "528 AO-Block voltage": "false", "TE2000-Description": "Nikon TE2000 hub", "528 AO-MinVolts": "0.0000", "COM4-Parity": "None", "Summary": { "Slices": 11, "ChContrastMin": [ 0, 0, 0, 0, 0, 0 ], "PixelAspect": 1, "SlicesFirst": true, "Interval_ms": 0, "Time": "2019-12-18 21:36:42 -0500", "Prefix": "seq_4", "InitialPositionList": [ { "Label": "Pos0", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.21], "Focus": [17741.5], "XYStage": [ 44542.200000000004, 31636.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos1", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.22], "Focus": [17741.4], "XYStage": [ 43801.100000000006, 31637.9 ] }, "GridColumnIndex": 0 }, { "Label": "Pos2", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.5], "XYStage": [ 38561.3, 32040.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos3", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.185], "Focus": [17741.3], "XYStage": [ 37698.4, 32041.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos4", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.16], "Focus": [17741.5], "XYStage": [ 32375.4, 31758.9 ] }, "GridColumnIndex": 0 }, { "Label": "Pos5", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.17000000000002], "Focus": [17741.3], "XYStage": [ 31770.7, 31758.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos6", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.4], "XYStage": [ 26536.800000000003, 31068.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos7", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.205], "Focus": [17741.4], "XYStage": [ 25420.2, 31068.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos8", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.85], "Focus": [17741.3], "XYStage": [ 21059, 31508 ] }, "GridColumnIndex": 0 }, { "Label": "Pos9", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.82], "Focus": [17741.3], "XYStage": [ 19731.9, 31506.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos10", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.79], "Focus": [17741.4], "XYStage": [ 14528.5, 32007.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos11", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.83], "Focus": [17741.2], "XYStage": [ 13830.300000000001, 32009.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos12", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.79], "Focus": [17741.3], "XYStage": [ 8775.300000000001, 32017.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos13", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.815], "Focus": [17741.3], "XYStage": [ 8109.200000000001, 32008.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos14", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.805], "Focus": [17741.3], "XYStage": [ 3483.3, 31908.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos15", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.82], "Focus": [17741.2], "XYStage": [ 2686.8, 31903.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos16", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [211.48000000000002], "Focus": [17741.1], "XYStage": [ -1978.9, 31973.600000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos17", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [211.51500000000001], "Focus": [17741.1], "XYStage": [ -2957.8, 31982.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos18", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.195], "Focus": [17741.5], "XYStage": [ 47747.4, -576.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos19", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.23000000000002], "Focus": [17741.6], "XYStage": [ 47053.9, -576.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos20", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.205], "Focus": [17741.4], "XYStage": [ 41871.200000000004, -479.8 ] }, "GridColumnIndex": 0 }, { "Label": "Pos21", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.4], "XYStage": [ 40882.600000000006, -392 ] }, "GridColumnIndex": 0 }, { "Label": "Pos22", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.24], "Focus": [17741.4], "XYStage": [ 35905.6, -135.3 ] }, "GridColumnIndex": 0 }, { "Label": "Pos23", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.195], "Focus": [17741.5], "XYStage": [ 34990.9, -132.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos24", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.46], "Focus": [17741.4], "XYStage": [ 29678.100000000002, -958.1 ] }, "GridColumnIndex": 0 }, { "Label": "Pos25", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.44], "Focus": [17741.5], "XYStage": [ 28983.600000000002, -939.4000000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos26", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.475], "Focus": [17741.5], "XYStage": [ 23019.7, -122 ] }, "GridColumnIndex": 0 }, { "Label": "Pos27", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.44], "Focus": [17741.7], "XYStage": [ 22428, -116.80000000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos28", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.46], "Focus": [17741.7], "XYStage": [ 16364.900000000001, -181.10000000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos29", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.48000000000002], "Focus": [17741.5], "XYStage": [ 15712.1, -180 ] }, "GridColumnIndex": 0 }, { "Label": "Pos30", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.45000000000002], "Focus": [17741.7], "XYStage": [ 10346.1, 77.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos31", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.49], "Focus": [17741.4], "XYStage": [ 9755.5, 85.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos32", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.475], "Focus": [17741.5], "XYStage": [ 4775.5, 293.90000000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos33", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.445], "Focus": [17741.4], "XYStage": [ 3954.9, 293.90000000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos34", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.455], "Focus": [17741.4], "XYStage": [ -1324.9, 545.6 ] }, "GridColumnIndex": 0 }, { "Label": "Pos35", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.445], "Focus": [17741.3], "XYStage": [ -2097, 576.6 ] }, "GridColumnIndex": 0 } ], "z-step_um": 10, "GridRow": 0, "BitDepth": 16, "Depth": 2, "Channels": 6, "Source": "Micro-Manager", "ChColors": [ -16711681, -256, -65281, -1, -1, -16776961 ], "MetadataVersion": 10, "KeepShutterOpenChannels": true, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536, 65536 ], "CustomIntervals_ms": [], "Height": 1200, "MicroManagerVersion": "1.4.23 20180219", "GridColumn": 0, "UUID": "14a13bf7-c944-4672-b873-fcfbbb7ea13d", "PixelType": "GRAY16", "Width": 1200, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\Yan\\20191218-YC113-S7S8-miseq", "Frames": 1, "Comment": "", "UserName": "Nikon", "PixelSize_um": 1.1, "ROI": [ 0, 0, 1200, 1200 ], "Date": "2019-12-18", "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC", "P-DAPI-high" ], "KeepShutterOpenSlices": true, "IJType": 1, "ComputerName": "ZadorConfocal2", "Positions": 36, "PositionIndex": 9, "CameraTimeout": 20000, "TimeFirst": false }, "640 AO-Name": "AnalogIO", "Vincent-D3-Command": "Close" }, "FrameKey-0-4-0": { "COM4-StopBits": "1", "528 AO-MaxVolts": "5.0000", "XLIGHT Excitation Wheel-State": "1", "470 Shutter-Name": "AnalogIO", "Core-Shutter": "Vincent-D3", "555 AO-Demo": "No", "XLIGHT Dichroic-State": "2", "XYStage-StepSize-Y": "0.1000", "ZPositionUm": 198.82, "XYStage-StepSize-X": "0.1000", "PRIME-FirmwareVersion": "20.28", "PRIME-ClearCycles": "2", "520-Name": "DA Shutter", "405 Shutter-Name": "AnalogIO", "PIEZO-StepSize": "0.0050", "PRIME-ClearMode": "Pre-Exposure", "470 AO-MinVolts": "0.0000", "405 AO-SupportsTriggering": "1", "CameraChannelIndex": 0, "XYStage-StartSpeed": "500.0000", "COM9-StopBits": "2", "405 AO-Description": "NI DAC", "COM4-DelayBetweenCharsMs": "0.0000", "445 AO-Block voltage": "false", "520 AO-TriggerInputLine": "/Dev1/PFI0", "555 AO-Description": "NI DAC", "520 AO-TriggerSampleRate": "100000", "COM4-Verbose": "1", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM4-Name": "COM4", "405 Shutter-Volts": "0.0000", "Vincent-D3-Port": "COM1", "PRIME-PP 4 MIN ADU AFFECTED": "200", "470 Shutter-MinVolts": "0.0000", "PRIME-PP 3 ENABLED": "Yes", "470 AO-Description": "NI DAC", "PRIME-Description": "PVCAM API device adapter", "520 Shutter-MaxVolts": "5.0000", "520 Shutter-Volts": "0.0000", "528 AO-Sequenceable": "1", "PRIME-Binning": "1x1", "555 Shutter-MaxVolts": "5.0000", "528 Shutter-TriggerSequenceLength": "1000", "405 Shutter-Block voltage": "false", "XLIGHT Spin motor-State": "1", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "Vincent-D3-Time to open (ms)": "35.0000", "Vincent-D3-Time to close (ms)": "35.0000", "445 AO-TriggerSampleRate": "100000", "640 Shutter-IOChannel": "Dev1/ao14", "555 AO-Sequenceable": "1", "520 AO-Name": "AnalogIO", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "470 AO-Block voltage": "false", "COM1-Verbose": "1", "COM9-AnswerTimeout": "10000.0000", "528 Shutter-Name": "AnalogIO", "PRIME-Trigger Timeout (secs)": "10", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "NextFrame": 0, "470 AO-TriggerSequenceLength": "1000", "PRIME-TransposeXY": "0", "445 AO-TriggerSequenceLength": "1000", "COM1-Name": "COM1", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "COM9-DataBits": "8", "COM1-AnswerTimeout": "500.0000", "Camera": "", "TE2000-Version": "V2.50-V2.50-V2.10", "528 Shutter-Volts": "0.0000", "XLIGHT Hub-Name": "XLIGHT Hub", "PIEZO-Description": "Ludl stage driver adapter", "PositionName": "Pos9", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSampleRate": "100000", "PRIME-FanSpeedSetpoint": "High", "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "405 AO-TriggerSampleRate": "100000", "470 Shutter-Block voltage": "false", "470-State": "0", "COM3-Parity": "None", "405 AO-Volts": "0.0000", "PRIME-TransposeMirrorY": "0", "PRIME-ChipName": "GS144BSI", "470 AO-Volts": "0.0000", "PRIME-TransposeMirrorX": "0", "445 Shutter-SupportsTriggering": "0", "470 Shutter-SupportsTriggering": "0", "XLIGHT Hub-Description": "XLIGHT hub", "PRIME-PreampOffLimit": "10000", "470 AO-Sequenceable": "1", "528 Shutter-MaxVolts": "5.0000", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "Core-XYStage": "XYStage", "470 AO-TriggerSampleRate": "100000", "PRIME-Timing-PreTriggerDelayNs": "24672000.0000", "528-DA Device": "528 Shutter", "Focus-Description": "Nikon TE2000 focus adapter", "FilterBlock-State": "5", "520 AO-Demo": "No", "COM3-DelayBetweenCharsMs": "0.0000", "470 Shutter-Volts": "0.0000", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "PRIME-PP 2 THRESHOLD": "125", "PRIME-PP 4 THRESHOLD": "75", "XYStage-Acceleration": "4.0000", "470 Shutter-Demo": "No", "PRIME-Name": "PMPCIECam00", "640-DA Device": "640 Shutter", "405 AO-Block voltage": "false", "ElapsedTime-ms": 402538, "PRIME-Offset": "100", "445 AO-IOChannel": "Dev1/ao1", "640 AO-MaxVolts": "5.0000", "PositionIndex": 9, "PRIME-PP 1 ENABLED": "Yes", "COM1-BaudRate": "9600", "640-State": "0", "COM3-Verbose": "1", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "LudlController-TransmissionDelay": "0", "PRIME-FullWellCapacity": "62000", "PRIME-SMARTStreamingEnabled": "No", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "640 Shutter-Description": "NI DAC", "528-Description": "DA device that is used as a shutter", "COM4-Description": "Serial port driver (boost:asio)", "405 Shutter-Description": "NI DAC", "TE2000-Name": "TE2000", "528 Shutter-Description": "NI DAC", "COM9-Description": "Serial port driver (boost:asio)", "LudlController-Name": "LudlController", "445 Shutter-TriggerSequenceLength": "1000", "PRIME-SerialNumber": "A18B203008", "445 AO-Volts": "0.0000", "PRIME-X-dimension": "1200", "PRIME-ShutterCloseDelay": "0", "XLIGHT Disk slider-State": "2", "Position": "Default", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 AO-IOChannel": "Dev1/ao5", "520 AO-IOChannel": "Dev1/ao4", "528 AO-TriggerSequenceLength": "1000", "405 Shutter-MaxVolts": "5.0000", "470-DA Device": "470 Shutter", "PRIME-FTCapable": "No", "Core-ChannelGroup": "Channels", "555 AO-SupportsTriggering": "0", "PRIME-Y-dimension": "1200", "Frame": 0, "555 Shutter-Sequenceable": "1", "PRIME-Timing-ExposureTimeNs": "499998000.0000", "Core-ImageProcessor": "", "528-State": "0", "520 AO-Block voltage": "false", "XLIGHT Excitation Wheel-Label": "Excitation-2", "640 Shutter-Demo": "No", "COM3-StopBits": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "520 Shutter-IOChannel": "Dev1/ao11", "Width": 1200, "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-SupportsTriggering": "0", "640 AO-TriggerSampleRate": "100000", "520-Description": "DA device that is used as a shutter", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "405 Shutter-Sequenceable": "1", "405 Shutter-SupportsTriggering": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-TriggerSampleRate": "100000", "640 Shutter-MaxVolts": "5.0000", "555 AO-MaxVolts": "5.0000", "520 AO-TriggerSequenceLength": "1000", "COM3-BaudRate": "9600", "COM9-BaudRate": "9600", "Vincent-D3-Channel #": "Ch. #1", "PRIME-PMode": "Normal", "OpticalPath-Label": "Left(100)", "BitDepth": 16, "555 AO-TriggerSequenceLength": "1000", "445 Shutter-Description": "NI DAC", "405 AO-Sequenceable": "1", "PRIME-AcquisitionMethod": "Callbacks", "555 AO-Name": "AnalogIO", "PRIME-ShutterMode": "Pre-Exposure", "COM3-Handshaking": "Off", "445 AO-SupportsTriggering": "0", "ROI": "0-0-1200-1200", "COM9-Verbose": "1", "PRIME-Color": "OFF", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "PRIME-ShutterOpenDelay": "0", "COM1-Parity": "None", "Slice": 0, "SlicePosition": -50, "COM9-DelayBetweenCharsMs": "0.0000", "555-Name": "DA Shutter", "PIEZO-LudlSingleAxisName": "B", "XLIGHT Emission Wheel-Label": "Empty", "640 Shutter-Name": "AnalogIO", "640 AO-Sequenceable": "1", "520 Shutter-Demo": "No", "528 Shutter-Demo": "No", "470-Name": "DA Shutter", "520-DA Device": "520 Shutter", "XYStage-Description": "Ludl XY stage driver adapter", "445 Shutter-MinVolts": "0.0000", "528 AO-TriggerSampleRate": "100000", "405 AO-MinVolts": "0.0000", "405 Shutter-TriggerSequenceLength": "1000", "Height": 1200, "555 AO-Block voltage": "false", "640 Shutter-Volts": "0.0000", "PixelType": "GRAY16", "445 Shutter-Block voltage": "false", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "520 Shutter-TriggerSequenceLength": "1000", "COM9-Name": "COM9", "Core-SLM": "", "555 AO-Volts": "0.0000", "555 Shutter-Block voltage": "false", "XYStage-Speed": "1800.0000", "520 AO-MaxVolts": "5.0000", "528 Shutter-SupportsTriggering": "0", "COM1-Description": "Serial port driver (boost:asio)", "PRIME-MetadataEnabled": "No", "520 Shutter-MinVolts": "0.0000", "470 Shutter-Sequenceable": "1", "PRIME-PreampDelay": "0", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-MinVolts": "0.0000", "ChannelIndex": 4, "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Core-AutoShutter": "1", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "Exposure-ms": 20, "PRIME-PP 2 ENABLED": "Yes", "640 Shutter-TriggerSampleRate": "100000", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "470 Shutter-IOChannel": "Dev1/ao10", "405 AO-Demo": "No", "PixelSizeUm": 1.1, "470 AO-Demo": "No", "470 Shutter-MaxVolts": "5.0000", "Vincent-D3-Name": "Vincent-D3", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FileName": "img_000000000_P-DIC_000.tif", "LudlController-Reset": "Operate", "PRIME-Exposure": "20.00", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "445-Name": "DA Shutter", "555 Shutter-Name": "AnalogIO", "520 Shutter-TriggerSampleRate": "100000", "445 Shutter-IOChannel": "Dev1/ao9", "PRIME-TransposeCorrection": "0", "XYStage-StepSize": "0.1000", "445-Description": "DA device that is used as a shutter", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 AO-IOChannel": "Dev1/ao7", "520 Shutter-Name": "AnalogIO", "COM3-DataBits": "8", "640 AO-Volts": "0.0000", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "555 Shutter-IOChannel": "Dev1/ao13", "WaitInterval": null, "520 Shutter-Sequenceable": "1", "YPositionUm": 31506.2, "445-State": "0", "445-DA Device": "445 Shutter", "PRIME-CircularBufferAutoSize": "ON", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "555 AO-TriggerSampleRate": "100000", "445 Shutter-Volts": "0.0000", "XLIGHT Hub-Port": "COM3", "Core-AutoFocus": "", "COM4-Handshaking": "Off", "528 Shutter-MinVolts": "0.0000", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "445 Shutter-Name": "AnalogIO", "528 Shutter-Sequenceable": "1", "XYStage-TransposeMirrorX": "0", "XYStage-TransposeMirrorY": "0", "PRIME-ReadoutRate": "100MHz 16bit", "XYStage-Name": "Stage", "640 AO-SupportsTriggering": "0", "640-Name": "DA Shutter", "640 Shutter-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "640 AO-Block voltage": "false", "445 Shutter-Sequenceable": "1", "470 AO-MaxVolts": "5.0000", "COM4-BaudRate": "9600", "470-Description": "DA device that is used as a shutter", "520 Shutter-Description": "NI DAC", "PRIME-CCDTemperature": "-14.8500", "Time": "2019-12-18 21:37:10 -0500", "640 AO-TriggerSequenceLength": "1000", "470 AO-Name": "AnalogIO", "XLIGHT Disk slider-Label": "Disk Pos 40um", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "Binning": "1x1", "TE2000-ModelType": "1", "COM3-AnswerTimeout": "500.0000", "555 Shutter-SupportsTriggering": "0", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "TE2000-Port": "COM4", "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Description": "NI DAC", "COM4-AnswerTimeout": "500.0000", "COM1-DelayBetweenCharsMs": "0.0000", "FilterBlock-Label": "Confocal", "445 AO-Name": "AnalogIO", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-Timing-ReadoutTimeNs": "24672000.0000", "528 AO-Demo": "No", "520 AO-Sequenceable": "1", "555-Description": "DA device that is used as a shutter", "405-DA Device": "405 Shutter", "405 AO-TriggerSequenceLength": "1000", "640 AO-MinVolts": "0.0000", "640 Shutter-Block voltage": "false", "FrameIndex": 0, "PRIME-Port": "CMOS", "555 AO-MinVolts": "0.0000", "COM3-Description": "Serial port driver (boost:asio)", "528 AO-Description": "NI DAC", "555 AO-IOChannel": "Dev1/ao6", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "555 AO-TriggerInputLine": "/Dev1/PFI0", "PRIME-ActualInterval-ms": "390.5200", "LudlController-Port": "COM9", "445 Shutter-Demo": "No", "PRIME-Trigger-Expose Out-Mux": "1", "SliceIndex": 0, "PRIME-CCDTemperatureSetPoint": "-15.0000", "405 Shutter-MinVolts": "0.0000", "PRIME-ExposeOutMode": "First Row", "AxisPositions": null, "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Volts": "0.0000", "528-Name": "DA Shutter", "555-State": "0", "XLIGHT Dichroic-Label": "Di03-R635-t1", "445 AO-Sequenceable": "1", "405-Name": "DA Shutter", "405 AO-IOChannel": "Dev1/ao0", "Core-Camera": "PRIME", "Focus-Name": "Focus", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "405 Shutter-IOChannel": "Dev1/ao8", "640 AO-Demo": "No", "640 Shutter-SupportsTriggering": "0", "445 AO-Demo": "No", "555-DA Device": "555 Shutter", "470 Shutter-Description": "NI DAC", "528 AO-Volts": "0.0000", "COM1-DataBits": "8", "Vincent-D3-Address": "x", "520 AO-SupportsTriggering": "0", "405-Description": "DA device that is used as a shutter", "640 Shutter-MinVolts": "0.0000", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "405 AO-Name": "AnalogIO", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "640 AO-TriggerInputLine": "/Dev1/PFI0", "405 AO-TriggerInputLine": "/Dev1/PFI0", "640-Description": "DA device that is used as a shutter", "445 Shutter-TriggerSampleRate": "100000", "470 Shutter-TriggerSequenceLength": "1000", "COM1-StopBits": "1", "520 Shutter-SupportsTriggering": "0", "405-State": "0", "Core-Initialize": "1", "XLIGHT Emission Wheel-State": "3", "520 AO-Description": "NI DAC", "528 AO-TriggerInputLine": "/Dev1/PFI0", "445 Shutter-MaxVolts": "5.0000", "COM9-Handshaking": "Off", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "XLIGHT Spin motor-Label": "On (spinning)", "PRIME-PP 5 ENABLED": "No", "UUID": "84b230e9-33b9-4837-85e7-61346c4190ed", "PRIME-TriggerMode": "Internal Trigger", "COM1-Handshaking": "Off", "PRIME-Timing-PostTriggerDelayNs": "49344000.0000", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "Focus-Position": "17741.5000", "528 Shutter-Block voltage": "false", "Channel": "P-DIC", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "555 Shutter-Demo": "No", "405 Shutter-Demo": "No", "528 AO-Name": "AnalogIO", "520-State": "0", "Core-Galvo": "", "405 Shutter-TriggerSampleRate": "100000", "Focus-FocusStepSize_(nm)": "100", "PRIME-PixelType": "16bit", "COM4-DataBits": "8", "470 AO-IOChannel": "Dev1/ao2", "520 AO-MinVolts": "0.0000", "COM9-Parity": "None", "PRIME-CircularBufferEnabled": "ON", "PRIME-PP 6 Reset": "No", "640 AO-Description": "NI DAC", "Source": "PRIME", "445 AO-MinVolts": "0.0000", "PIEZO-Name": "Stage", "445 AO-Description": "NI DAC", "PRIME-CircularBufferFrameCount": "46", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "520 AO-Volts": "0.0000", "528 Shutter-TriggerSampleRate": "100000", "XPositionUm": 19731.9, "OpticalPath-State": "4", "PRIME-PP 3 THRESHOLD": "80", "PRIME-PVCAM Version": "3.7.1", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Gain": "1-HDR", "640 Shutter-TriggerSequenceLength": "1000", "528 AO-SupportsTriggering": "0", "445 AO-TriggerInputLine": "/Dev1/PFI0", "OpticalPath-Name": "OpticalPath", "PRIME-PP 4 ENABLED": "Yes", "PRIME-PVCAM Adapter Version": "1.3.29", "COM3-Name": "COM3", "405 AO-MaxVolts": "5.0000", "520 Shutter-Block voltage": "false", "PRIME-Timing-ClearingTimeNs": "49344000.0000", "Core-Focus": "PIEZO", "528 AO-Block voltage": "false", "TE2000-Description": "Nikon TE2000 hub", "528 AO-MinVolts": "0.0000", "COM4-Parity": "None", "Summary": { "Slices": 11, "ChContrastMin": [ 0, 0, 0, 0, 0, 0 ], "PixelAspect": 1, "SlicesFirst": true, "Interval_ms": 0, "Time": "2019-12-18 21:36:42 -0500", "Prefix": "seq_4", "InitialPositionList": [ { "Label": "Pos0", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.21], "Focus": [17741.5], "XYStage": [ 44542.200000000004, 31636.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos1", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.22], "Focus": [17741.4], "XYStage": [ 43801.100000000006, 31637.9 ] }, "GridColumnIndex": 0 }, { "Label": "Pos2", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.5], "XYStage": [ 38561.3, 32040.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos3", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.185], "Focus": [17741.3], "XYStage": [ 37698.4, 32041.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos4", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.16], "Focus": [17741.5], "XYStage": [ 32375.4, 31758.9 ] }, "GridColumnIndex": 0 }, { "Label": "Pos5", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.17000000000002], "Focus": [17741.3], "XYStage": [ 31770.7, 31758.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos6", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.4], "XYStage": [ 26536.800000000003, 31068.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos7", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.205], "Focus": [17741.4], "XYStage": [ 25420.2, 31068.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos8", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.85], "Focus": [17741.3], "XYStage": [ 21059, 31508 ] }, "GridColumnIndex": 0 }, { "Label": "Pos9", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.82], "Focus": [17741.3], "XYStage": [ 19731.9, 31506.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos10", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.79], "Focus": [17741.4], "XYStage": [ 14528.5, 32007.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos11", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.83], "Focus": [17741.2], "XYStage": [ 13830.300000000001, 32009.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos12", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.79], "Focus": [17741.3], "XYStage": [ 8775.300000000001, 32017.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos13", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.815], "Focus": [17741.3], "XYStage": [ 8109.200000000001, 32008.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos14", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.805], "Focus": [17741.3], "XYStage": [ 3483.3, 31908.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos15", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.82], "Focus": [17741.2], "XYStage": [ 2686.8, 31903.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos16", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [211.48000000000002], "Focus": [17741.1], "XYStage": [ -1978.9, 31973.600000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos17", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [211.51500000000001], "Focus": [17741.1], "XYStage": [ -2957.8, 31982.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos18", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.195], "Focus": [17741.5], "XYStage": [ 47747.4, -576.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos19", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.23000000000002], "Focus": [17741.6], "XYStage": [ 47053.9, -576.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos20", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.205], "Focus": [17741.4], "XYStage": [ 41871.200000000004, -479.8 ] }, "GridColumnIndex": 0 }, { "Label": "Pos21", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.4], "XYStage": [ 40882.600000000006, -392 ] }, "GridColumnIndex": 0 }, { "Label": "Pos22", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.24], "Focus": [17741.4], "XYStage": [ 35905.6, -135.3 ] }, "GridColumnIndex": 0 }, { "Label": "Pos23", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.195], "Focus": [17741.5], "XYStage": [ 34990.9, -132.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos24", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.46], "Focus": [17741.4], "XYStage": [ 29678.100000000002, -958.1 ] }, "GridColumnIndex": 0 }, { "Label": "Pos25", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.44], "Focus": [17741.5], "XYStage": [ 28983.600000000002, -939.4000000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos26", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.475], "Focus": [17741.5], "XYStage": [ 23019.7, -122 ] }, "GridColumnIndex": 0 }, { "Label": "Pos27", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.44], "Focus": [17741.7], "XYStage": [ 22428, -116.80000000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos28", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.46], "Focus": [17741.7], "XYStage": [ 16364.900000000001, -181.10000000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos29", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.48000000000002], "Focus": [17741.5], "XYStage": [ 15712.1, -180 ] }, "GridColumnIndex": 0 }, { "Label": "Pos30", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.45000000000002], "Focus": [17741.7], "XYStage": [ 10346.1, 77.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos31", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.49], "Focus": [17741.4], "XYStage": [ 9755.5, 85.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos32", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.475], "Focus": [17741.5], "XYStage": [ 4775.5, 293.90000000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos33", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.445], "Focus": [17741.4], "XYStage": [ 3954.9, 293.90000000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos34", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.455], "Focus": [17741.4], "XYStage": [ -1324.9, 545.6 ] }, "GridColumnIndex": 0 }, { "Label": "Pos35", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.445], "Focus": [17741.3], "XYStage": [ -2097, 576.6 ] }, "GridColumnIndex": 0 } ], "z-step_um": 10, "GridRow": 0, "BitDepth": 16, "Depth": 2, "Channels": 6, "Source": "Micro-Manager", "ChColors": [ -16711681, -256, -65281, -1, -1, -16776961 ], "MetadataVersion": 10, "KeepShutterOpenChannels": true, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536, 65536 ], "CustomIntervals_ms": [], "Height": 1200, "MicroManagerVersion": "1.4.23 20180219", "GridColumn": 0, "UUID": "14a13bf7-c944-4672-b873-fcfbbb7ea13d", "PixelType": "GRAY16", "Width": 1200, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\Yan\\20191218-YC113-S7S8-miseq", "Frames": 1, "Comment": "", "UserName": "Nikon", "PixelSize_um": 1.1, "ROI": [ 0, 0, 1200, 1200 ], "Date": "2019-12-18", "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC", "P-DAPI-high" ], "KeepShutterOpenSlices": true, "IJType": 1, "ComputerName": "ZadorConfocal2", "Positions": 36, "PositionIndex": 9, "CameraTimeout": 20000, "TimeFirst": false }, "640 AO-Name": "AnalogIO", "Vincent-D3-Command": "Close" }, "FrameKey-0-4-1": { "COM4-StopBits": "1", "528 AO-MaxVolts": "5.0000", "XLIGHT Excitation Wheel-State": "1", "470 Shutter-Name": "AnalogIO", "Core-Shutter": "Vincent-D3", "555 AO-Demo": "No", "XLIGHT Dichroic-State": "2", "XYStage-StepSize-Y": "0.1000", "ZPositionUm": 208.82, "XYStage-StepSize-X": "0.1000", "PRIME-FirmwareVersion": "20.28", "PRIME-ClearCycles": "2", "520-Name": "DA Shutter", "405 Shutter-Name": "AnalogIO", "PIEZO-StepSize": "0.0050", "PRIME-ClearMode": "Pre-Exposure", "470 AO-MinVolts": "0.0000", "405 AO-SupportsTriggering": "1", "CameraChannelIndex": 0, "XYStage-StartSpeed": "500.0000", "COM9-StopBits": "2", "405 AO-Description": "NI DAC", "COM4-DelayBetweenCharsMs": "0.0000", "445 AO-Block voltage": "false", "520 AO-TriggerInputLine": "/Dev1/PFI0", "555 AO-Description": "NI DAC", "520 AO-TriggerSampleRate": "100000", "COM4-Verbose": "1", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM4-Name": "COM4", "405 Shutter-Volts": "0.0000", "Vincent-D3-Port": "COM1", "PRIME-PP 4 MIN ADU AFFECTED": "200", "470 Shutter-MinVolts": "0.0000", "PRIME-PP 3 ENABLED": "Yes", "470 AO-Description": "NI DAC", "PRIME-Description": "PVCAM API device adapter", "520 Shutter-MaxVolts": "5.0000", "520 Shutter-Volts": "0.0000", "528 AO-Sequenceable": "1", "PRIME-Binning": "1x1", "555 Shutter-MaxVolts": "5.0000", "528 Shutter-TriggerSequenceLength": "1000", "405 Shutter-Block voltage": "false", "XLIGHT Spin motor-State": "1", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "Vincent-D3-Time to open (ms)": "35.0000", "Vincent-D3-Time to close (ms)": "35.0000", "445 AO-TriggerSampleRate": "100000", "640 Shutter-IOChannel": "Dev1/ao14", "555 AO-Sequenceable": "1", "520 AO-Name": "AnalogIO", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "470 AO-Block voltage": "false", "COM1-Verbose": "1", "COM9-AnswerTimeout": "10000.0000", "528 Shutter-Name": "AnalogIO", "PRIME-Trigger Timeout (secs)": "10", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "NextFrame": 0, "470 AO-TriggerSequenceLength": "1000", "PRIME-TransposeXY": "0", "445 AO-TriggerSequenceLength": "1000", "COM1-Name": "COM1", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "COM9-DataBits": "8", "COM1-AnswerTimeout": "500.0000", "Camera": "", "TE2000-Version": "V2.50-V2.50-V2.10", "528 Shutter-Volts": "0.0000", "XLIGHT Hub-Name": "XLIGHT Hub", "PIEZO-Description": "Ludl stage driver adapter", "PositionName": "Pos9", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSampleRate": "100000", "PRIME-FanSpeedSetpoint": "High", "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "405 AO-TriggerSampleRate": "100000", "470 Shutter-Block voltage": "false", "470-State": "0", "COM3-Parity": "None", "405 AO-Volts": "0.0000", "PRIME-TransposeMirrorY": "0", "PRIME-ChipName": "GS144BSI", "470 AO-Volts": "0.0000", "PRIME-TransposeMirrorX": "0", "445 Shutter-SupportsTriggering": "0", "470 Shutter-SupportsTriggering": "0", "XLIGHT Hub-Description": "XLIGHT hub", "PRIME-PreampOffLimit": "10000", "470 AO-Sequenceable": "1", "528 Shutter-MaxVolts": "5.0000", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "Core-XYStage": "XYStage", "470 AO-TriggerSampleRate": "100000", "PRIME-Timing-PreTriggerDelayNs": "24672000.0000", "528-DA Device": "528 Shutter", "Focus-Description": "Nikon TE2000 focus adapter", "FilterBlock-State": "5", "520 AO-Demo": "No", "COM3-DelayBetweenCharsMs": "0.0000", "470 Shutter-Volts": "0.0000", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "PRIME-PP 2 THRESHOLD": "125", "PRIME-PP 4 THRESHOLD": "75", "XYStage-Acceleration": "4.0000", "470 Shutter-Demo": "No", "PRIME-Name": "PMPCIECam00", "640-DA Device": "640 Shutter", "405 AO-Block voltage": "false", "ElapsedTime-ms": 402924, "PRIME-Offset": "100", "445 AO-IOChannel": "Dev1/ao1", "640 AO-MaxVolts": "5.0000", "PositionIndex": 9, "PRIME-PP 1 ENABLED": "Yes", "COM1-BaudRate": "9600", "640-State": "0", "COM3-Verbose": "1", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "LudlController-TransmissionDelay": "0", "PRIME-FullWellCapacity": "62000", "PRIME-SMARTStreamingEnabled": "No", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "640 Shutter-Description": "NI DAC", "528-Description": "DA device that is used as a shutter", "COM4-Description": "Serial port driver (boost:asio)", "405 Shutter-Description": "NI DAC", "TE2000-Name": "TE2000", "528 Shutter-Description": "NI DAC", "COM9-Description": "Serial port driver (boost:asio)", "LudlController-Name": "LudlController", "445 Shutter-TriggerSequenceLength": "1000", "PRIME-SerialNumber": "A18B203008", "445 AO-Volts": "0.0000", "PRIME-X-dimension": "1200", "PRIME-ShutterCloseDelay": "0", "XLIGHT Disk slider-State": "2", "Position": "Default", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 AO-IOChannel": "Dev1/ao5", "520 AO-IOChannel": "Dev1/ao4", "528 AO-TriggerSequenceLength": "1000", "405 Shutter-MaxVolts": "5.0000", "470-DA Device": "470 Shutter", "PRIME-FTCapable": "No", "Core-ChannelGroup": "Channels", "555 AO-SupportsTriggering": "0", "PRIME-Y-dimension": "1200", "Frame": 0, "555 Shutter-Sequenceable": "1", "PRIME-Timing-ExposureTimeNs": "499998000.0000", "Core-ImageProcessor": "", "528-State": "0", "520 AO-Block voltage": "false", "XLIGHT Excitation Wheel-Label": "Excitation-2", "640 Shutter-Demo": "No", "COM3-StopBits": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "520 Shutter-IOChannel": "Dev1/ao11", "Width": 1200, "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-SupportsTriggering": "0", "640 AO-TriggerSampleRate": "100000", "520-Description": "DA device that is used as a shutter", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "405 Shutter-Sequenceable": "1", "405 Shutter-SupportsTriggering": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-TriggerSampleRate": "100000", "640 Shutter-MaxVolts": "5.0000", "555 AO-MaxVolts": "5.0000", "520 AO-TriggerSequenceLength": "1000", "COM3-BaudRate": "9600", "COM9-BaudRate": "9600", "Vincent-D3-Channel #": "Ch. #1", "PRIME-PMode": "Normal", "OpticalPath-Label": "Left(100)", "BitDepth": 16, "555 AO-TriggerSequenceLength": "1000", "445 Shutter-Description": "NI DAC", "405 AO-Sequenceable": "1", "PRIME-AcquisitionMethod": "Callbacks", "555 AO-Name": "AnalogIO", "PRIME-ShutterMode": "Pre-Exposure", "COM3-Handshaking": "Off", "445 AO-SupportsTriggering": "0", "ROI": "0-0-1200-1200", "COM9-Verbose": "1", "PRIME-Color": "OFF", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "PRIME-ShutterOpenDelay": "0", "COM1-Parity": "None", "Slice": 1, "SlicePosition": -40, "COM9-DelayBetweenCharsMs": "0.0000", "555-Name": "DA Shutter", "PIEZO-LudlSingleAxisName": "B", "XLIGHT Emission Wheel-Label": "Empty", "640 Shutter-Name": "AnalogIO", "640 AO-Sequenceable": "1", "520 Shutter-Demo": "No", "528 Shutter-Demo": "No", "470-Name": "DA Shutter", "520-DA Device": "520 Shutter", "XYStage-Description": "Ludl XY stage driver adapter", "445 Shutter-MinVolts": "0.0000", "528 AO-TriggerSampleRate": "100000", "405 AO-MinVolts": "0.0000", "405 Shutter-TriggerSequenceLength": "1000", "Height": 1200, "555 AO-Block voltage": "false", "640 Shutter-Volts": "0.0000", "PixelType": "GRAY16", "445 Shutter-Block voltage": "false", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "520 Shutter-TriggerSequenceLength": "1000", "COM9-Name": "COM9", "Core-SLM": "", "555 AO-Volts": "0.0000", "555 Shutter-Block voltage": "false", "XYStage-Speed": "1800.0000", "520 AO-MaxVolts": "5.0000", "528 Shutter-SupportsTriggering": "0", "COM1-Description": "Serial port driver (boost:asio)", "PRIME-MetadataEnabled": "No", "520 Shutter-MinVolts": "0.0000", "470 Shutter-Sequenceable": "1", "PRIME-PreampDelay": "0", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-MinVolts": "0.0000", "ChannelIndex": 4, "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Core-AutoShutter": "1", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "Exposure-ms": 20, "PRIME-PP 2 ENABLED": "Yes", "640 Shutter-TriggerSampleRate": "100000", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "470 Shutter-IOChannel": "Dev1/ao10", "405 AO-Demo": "No", "PixelSizeUm": 1.1, "470 AO-Demo": "No", "470 Shutter-MaxVolts": "5.0000", "Vincent-D3-Name": "Vincent-D3", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FileName": "img_000000000_P-DIC_001.tif", "LudlController-Reset": "Operate", "PRIME-Exposure": "20.00", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "445-Name": "DA Shutter", "555 Shutter-Name": "AnalogIO", "520 Shutter-TriggerSampleRate": "100000", "445 Shutter-IOChannel": "Dev1/ao9", "PRIME-TransposeCorrection": "0", "XYStage-StepSize": "0.1000", "445-Description": "DA device that is used as a shutter", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 AO-IOChannel": "Dev1/ao7", "520 Shutter-Name": "AnalogIO", "COM3-DataBits": "8", "640 AO-Volts": "0.0000", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "555 Shutter-IOChannel": "Dev1/ao13", "WaitInterval": null, "520 Shutter-Sequenceable": "1", "YPositionUm": 31506.2, "445-State": "0", "445-DA Device": "445 Shutter", "PRIME-CircularBufferAutoSize": "ON", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "555 AO-TriggerSampleRate": "100000", "445 Shutter-Volts": "0.0000", "XLIGHT Hub-Port": "COM3", "Core-AutoFocus": "", "COM4-Handshaking": "Off", "528 Shutter-MinVolts": "0.0000", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "445 Shutter-Name": "AnalogIO", "528 Shutter-Sequenceable": "1", "XYStage-TransposeMirrorX": "0", "XYStage-TransposeMirrorY": "0", "PRIME-ReadoutRate": "100MHz 16bit", "XYStage-Name": "Stage", "640 AO-SupportsTriggering": "0", "640-Name": "DA Shutter", "640 Shutter-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "640 AO-Block voltage": "false", "445 Shutter-Sequenceable": "1", "470 AO-MaxVolts": "5.0000", "COM4-BaudRate": "9600", "470-Description": "DA device that is used as a shutter", "520 Shutter-Description": "NI DAC", "PRIME-CCDTemperature": "-14.8500", "Time": "2019-12-18 21:37:10 -0500", "640 AO-TriggerSequenceLength": "1000", "470 AO-Name": "AnalogIO", "XLIGHT Disk slider-Label": "Disk Pos 40um", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "Binning": "1x1", "TE2000-ModelType": "1", "COM3-AnswerTimeout": "500.0000", "555 Shutter-SupportsTriggering": "0", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "TE2000-Port": "COM4", "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Description": "NI DAC", "COM4-AnswerTimeout": "500.0000", "COM1-DelayBetweenCharsMs": "0.0000", "FilterBlock-Label": "Confocal", "445 AO-Name": "AnalogIO", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-Timing-ReadoutTimeNs": "24672000.0000", "528 AO-Demo": "No", "520 AO-Sequenceable": "1", "555-Description": "DA device that is used as a shutter", "405-DA Device": "405 Shutter", "405 AO-TriggerSequenceLength": "1000", "640 AO-MinVolts": "0.0000", "640 Shutter-Block voltage": "false", "FrameIndex": 0, "PRIME-Port": "CMOS", "555 AO-MinVolts": "0.0000", "COM3-Description": "Serial port driver (boost:asio)", "528 AO-Description": "NI DAC", "555 AO-IOChannel": "Dev1/ao6", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "555 AO-TriggerInputLine": "/Dev1/PFI0", "PRIME-ActualInterval-ms": "390.5200", "LudlController-Port": "COM9", "445 Shutter-Demo": "No", "PRIME-Trigger-Expose Out-Mux": "1", "SliceIndex": 1, "PRIME-CCDTemperatureSetPoint": "-15.0000", "405 Shutter-MinVolts": "0.0000", "PRIME-ExposeOutMode": "First Row", "AxisPositions": null, "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Volts": "0.0000", "528-Name": "DA Shutter", "555-State": "0", "XLIGHT Dichroic-Label": "Di03-R635-t1", "445 AO-Sequenceable": "1", "405-Name": "DA Shutter", "405 AO-IOChannel": "Dev1/ao0", "Core-Camera": "PRIME", "Focus-Name": "Focus", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "405 Shutter-IOChannel": "Dev1/ao8", "640 AO-Demo": "No", "640 Shutter-SupportsTriggering": "0", "445 AO-Demo": "No", "555-DA Device": "555 Shutter", "470 Shutter-Description": "NI DAC", "528 AO-Volts": "0.0000", "COM1-DataBits": "8", "Vincent-D3-Address": "x", "520 AO-SupportsTriggering": "0", "405-Description": "DA device that is used as a shutter", "640 Shutter-MinVolts": "0.0000", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "405 AO-Name": "AnalogIO", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "640 AO-TriggerInputLine": "/Dev1/PFI0", "405 AO-TriggerInputLine": "/Dev1/PFI0", "640-Description": "DA device that is used as a shutter", "445 Shutter-TriggerSampleRate": "100000", "470 Shutter-TriggerSequenceLength": "1000", "COM1-StopBits": "1", "520 Shutter-SupportsTriggering": "0", "405-State": "0", "Core-Initialize": "1", "XLIGHT Emission Wheel-State": "3", "520 AO-Description": "NI DAC", "528 AO-TriggerInputLine": "/Dev1/PFI0", "445 Shutter-MaxVolts": "5.0000", "COM9-Handshaking": "Off", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "XLIGHT Spin motor-Label": "On (spinning)", "PRIME-PP 5 ENABLED": "No", "UUID": "659680bc-8167-49d6-a4fc-ae9986d0bb4f", "PRIME-TriggerMode": "Internal Trigger", "COM1-Handshaking": "Off", "PRIME-Timing-PostTriggerDelayNs": "49344000.0000", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "Focus-Position": "17741.5000", "528 Shutter-Block voltage": "false", "Channel": "P-DIC", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "555 Shutter-Demo": "No", "405 Shutter-Demo": "No", "528 AO-Name": "AnalogIO", "520-State": "0", "Core-Galvo": "", "405 Shutter-TriggerSampleRate": "100000", "Focus-FocusStepSize_(nm)": "100", "PRIME-PixelType": "16bit", "COM4-DataBits": "8", "470 AO-IOChannel": "Dev1/ao2", "520 AO-MinVolts": "0.0000", "COM9-Parity": "None", "PRIME-CircularBufferEnabled": "ON", "PRIME-PP 6 Reset": "No", "640 AO-Description": "NI DAC", "Source": "PRIME", "445 AO-MinVolts": "0.0000", "PIEZO-Name": "Stage", "445 AO-Description": "NI DAC", "PRIME-CircularBufferFrameCount": "46", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "520 AO-Volts": "0.0000", "528 Shutter-TriggerSampleRate": "100000", "XPositionUm": 19731.9, "OpticalPath-State": "4", "PRIME-PP 3 THRESHOLD": "80", "PRIME-PVCAM Version": "3.7.1", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Gain": "1-HDR", "640 Shutter-TriggerSequenceLength": "1000", "528 AO-SupportsTriggering": "0", "445 AO-TriggerInputLine": "/Dev1/PFI0", "OpticalPath-Name": "OpticalPath", "PRIME-PP 4 ENABLED": "Yes", "PRIME-PVCAM Adapter Version": "1.3.29", "COM3-Name": "COM3", "405 AO-MaxVolts": "5.0000", "520 Shutter-Block voltage": "false", "PRIME-Timing-ClearingTimeNs": "49344000.0000", "Core-Focus": "PIEZO", "528 AO-Block voltage": "false", "TE2000-Description": "Nikon TE2000 hub", "528 AO-MinVolts": "0.0000", "COM4-Parity": "None", "Summary": { "Slices": 11, "ChContrastMin": [ 0, 0, 0, 0, 0, 0 ], "PixelAspect": 1, "SlicesFirst": true, "Interval_ms": 0, "Time": "2019-12-18 21:36:42 -0500", "Prefix": "seq_4", "InitialPositionList": [ { "Label": "Pos0", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.21], "Focus": [17741.5], "XYStage": [ 44542.200000000004, 31636.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos1", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.22], "Focus": [17741.4], "XYStage": [ 43801.100000000006, 31637.9 ] }, "GridColumnIndex": 0 }, { "Label": "Pos2", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.5], "XYStage": [ 38561.3, 32040.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos3", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.185], "Focus": [17741.3], "XYStage": [ 37698.4, 32041.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos4", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.16], "Focus": [17741.5], "XYStage": [ 32375.4, 31758.9 ] }, "GridColumnIndex": 0 }, { "Label": "Pos5", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.17000000000002], "Focus": [17741.3], "XYStage": [ 31770.7, 31758.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos6", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.4], "XYStage": [ 26536.800000000003, 31068.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos7", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.205], "Focus": [17741.4], "XYStage": [ 25420.2, 31068.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos8", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.85], "Focus": [17741.3], "XYStage": [ 21059, 31508 ] }, "GridColumnIndex": 0 }, { "Label": "Pos9", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.82], "Focus": [17741.3], "XYStage": [ 19731.9, 31506.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos10", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.79], "Focus": [17741.4], "XYStage": [ 14528.5, 32007.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos11", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.83], "Focus": [17741.2], "XYStage": [ 13830.300000000001, 32009.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos12", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.79], "Focus": [17741.3], "XYStage": [ 8775.300000000001, 32017.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos13", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.815], "Focus": [17741.3], "XYStage": [ 8109.200000000001, 32008.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos14", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.805], "Focus": [17741.3], "XYStage": [ 3483.3, 31908.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos15", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.82], "Focus": [17741.2], "XYStage": [ 2686.8, 31903.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos16", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [211.48000000000002], "Focus": [17741.1], "XYStage": [ -1978.9, 31973.600000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos17", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [211.51500000000001], "Focus": [17741.1], "XYStage": [ -2957.8, 31982.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos18", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.195], "Focus": [17741.5], "XYStage": [ 47747.4, -576.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos19", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.23000000000002], "Focus": [17741.6], "XYStage": [ 47053.9, -576.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos20", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.205], "Focus": [17741.4], "XYStage": [ 41871.200000000004, -479.8 ] }, "GridColumnIndex": 0 }, { "Label": "Pos21", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.4], "XYStage": [ 40882.600000000006, -392 ] }, "GridColumnIndex": 0 }, { "Label": "Pos22", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.24], "Focus": [17741.4], "XYStage": [ 35905.6, -135.3 ] }, "GridColumnIndex": 0 }, { "Label": "Pos23", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.195], "Focus": [17741.5], "XYStage": [ 34990.9, -132.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos24", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.46], "Focus": [17741.4], "XYStage": [ 29678.100000000002, -958.1 ] }, "GridColumnIndex": 0 }, { "Label": "Pos25", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.44], "Focus": [17741.5], "XYStage": [ 28983.600000000002, -939.4000000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos26", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.475], "Focus": [17741.5], "XYStage": [ 23019.7, -122 ] }, "GridColumnIndex": 0 }, { "Label": "Pos27", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.44], "Focus": [17741.7], "XYStage": [ 22428, -116.80000000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos28", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.46], "Focus": [17741.7], "XYStage": [ 16364.900000000001, -181.10000000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos29", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.48000000000002], "Focus": [17741.5], "XYStage": [ 15712.1, -180 ] }, "GridColumnIndex": 0 }, { "Label": "Pos30", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.45000000000002], "Focus": [17741.7], "XYStage": [ 10346.1, 77.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos31", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.49], "Focus": [17741.4], "XYStage": [ 9755.5, 85.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos32", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.475], "Focus": [17741.5], "XYStage": [ 4775.5, 293.90000000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos33", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.445], "Focus": [17741.4], "XYStage": [ 3954.9, 293.90000000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos34", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.455], "Focus": [17741.4], "XYStage": [ -1324.9, 545.6 ] }, "GridColumnIndex": 0 }, { "Label": "Pos35", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.445], "Focus": [17741.3], "XYStage": [ -2097, 576.6 ] }, "GridColumnIndex": 0 } ], "z-step_um": 10, "GridRow": 0, "BitDepth": 16, "Depth": 2, "Channels": 6, "Source": "Micro-Manager", "ChColors": [ -16711681, -256, -65281, -1, -1, -16776961 ], "MetadataVersion": 10, "KeepShutterOpenChannels": true, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536, 65536 ], "CustomIntervals_ms": [], "Height": 1200, "MicroManagerVersion": "1.4.23 20180219", "GridColumn": 0, "UUID": "14a13bf7-c944-4672-b873-fcfbbb7ea13d", "PixelType": "GRAY16", "Width": 1200, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\Yan\\20191218-YC113-S7S8-miseq", "Frames": 1, "Comment": "", "UserName": "Nikon", "PixelSize_um": 1.1, "ROI": [ 0, 0, 1200, 1200 ], "Date": "2019-12-18", "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC", "P-DAPI-high" ], "KeepShutterOpenSlices": true, "IJType": 1, "ComputerName": "ZadorConfocal2", "Positions": 36, "PositionIndex": 9, "CameraTimeout": 20000, "TimeFirst": false }, "640 AO-Name": "AnalogIO", "Vincent-D3-Command": "Close" }, "FrameKey-0-4-2": { "COM4-StopBits": "1", "528 AO-MaxVolts": "5.0000", "XLIGHT Excitation Wheel-State": "1", "470 Shutter-Name": "AnalogIO", "Core-Shutter": "Vincent-D3", "555 AO-Demo": "No", "XLIGHT Dichroic-State": "2", "XYStage-StepSize-Y": "0.1000", "ZPositionUm": 218.82, "XYStage-StepSize-X": "0.1000", "PRIME-FirmwareVersion": "20.28", "PRIME-ClearCycles": "2", "520-Name": "DA Shutter", "405 Shutter-Name": "AnalogIO", "PIEZO-StepSize": "0.0050", "PRIME-ClearMode": "Pre-Exposure", "470 AO-MinVolts": "0.0000", "405 AO-SupportsTriggering": "1", "CameraChannelIndex": 0, "XYStage-StartSpeed": "500.0000", "COM9-StopBits": "2", "405 AO-Description": "NI DAC", "COM4-DelayBetweenCharsMs": "0.0000", "445 AO-Block voltage": "false", "520 AO-TriggerInputLine": "/Dev1/PFI0", "555 AO-Description": "NI DAC", "520 AO-TriggerSampleRate": "100000", "COM4-Verbose": "1", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM4-Name": "COM4", "405 Shutter-Volts": "0.0000", "Vincent-D3-Port": "COM1", "PRIME-PP 4 MIN ADU AFFECTED": "200", "470 Shutter-MinVolts": "0.0000", "PRIME-PP 3 ENABLED": "Yes", "470 AO-Description": "NI DAC", "PRIME-Description": "PVCAM API device adapter", "520 Shutter-MaxVolts": "5.0000", "520 Shutter-Volts": "0.0000", "528 AO-Sequenceable": "1", "PRIME-Binning": "1x1", "555 Shutter-MaxVolts": "5.0000", "528 Shutter-TriggerSequenceLength": "1000", "405 Shutter-Block voltage": "false", "XLIGHT Spin motor-State": "1", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "Vincent-D3-Time to open (ms)": "35.0000", "Vincent-D3-Time to close (ms)": "35.0000", "445 AO-TriggerSampleRate": "100000", "640 Shutter-IOChannel": "Dev1/ao14", "555 AO-Sequenceable": "1", "520 AO-Name": "AnalogIO", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "470 AO-Block voltage": "false", "COM1-Verbose": "1", "COM9-AnswerTimeout": "10000.0000", "528 Shutter-Name": "AnalogIO", "PRIME-Trigger Timeout (secs)": "10", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "NextFrame": 0, "470 AO-TriggerSequenceLength": "1000", "PRIME-TransposeXY": "0", "445 AO-TriggerSequenceLength": "1000", "COM1-Name": "COM1", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "COM9-DataBits": "8", "COM1-AnswerTimeout": "500.0000", "Camera": "", "TE2000-Version": "V2.50-V2.50-V2.10", "528 Shutter-Volts": "0.0000", "XLIGHT Hub-Name": "XLIGHT Hub", "PIEZO-Description": "Ludl stage driver adapter", "PositionName": "Pos9", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSampleRate": "100000", "PRIME-FanSpeedSetpoint": "High", "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "405 AO-TriggerSampleRate": "100000", "470 Shutter-Block voltage": "false", "470-State": "0", "COM3-Parity": "None", "405 AO-Volts": "0.0000", "PRIME-TransposeMirrorY": "0", "PRIME-ChipName": "GS144BSI", "470 AO-Volts": "0.0000", "PRIME-TransposeMirrorX": "0", "445 Shutter-SupportsTriggering": "0", "470 Shutter-SupportsTriggering": "0", "XLIGHT Hub-Description": "XLIGHT hub", "PRIME-PreampOffLimit": "10000", "470 AO-Sequenceable": "1", "528 Shutter-MaxVolts": "5.0000", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "Core-XYStage": "XYStage", "470 AO-TriggerSampleRate": "100000", "PRIME-Timing-PreTriggerDelayNs": "24672000.0000", "528-DA Device": "528 Shutter", "Focus-Description": "Nikon TE2000 focus adapter", "FilterBlock-State": "5", "520 AO-Demo": "No", "COM3-DelayBetweenCharsMs": "0.0000", "470 Shutter-Volts": "0.0000", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "PRIME-PP 2 THRESHOLD": "125", "PRIME-PP 4 THRESHOLD": "75", "XYStage-Acceleration": "4.0000", "470 Shutter-Demo": "No", "PRIME-Name": "PMPCIECam00", "640-DA Device": "640 Shutter", "405 AO-Block voltage": "false", "ElapsedTime-ms": 403245, "PRIME-Offset": "100", "445 AO-IOChannel": "Dev1/ao1", "640 AO-MaxVolts": "5.0000", "PositionIndex": 9, "PRIME-PP 1 ENABLED": "Yes", "COM1-BaudRate": "9600", "640-State": "0", "COM3-Verbose": "1", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "LudlController-TransmissionDelay": "0", "PRIME-FullWellCapacity": "62000", "PRIME-SMARTStreamingEnabled": "No", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "640 Shutter-Description": "NI DAC", "528-Description": "DA device that is used as a shutter", "COM4-Description": "Serial port driver (boost:asio)", "405 Shutter-Description": "NI DAC", "TE2000-Name": "TE2000", "528 Shutter-Description": "NI DAC", "COM9-Description": "Serial port driver (boost:asio)", "LudlController-Name": "LudlController", "445 Shutter-TriggerSequenceLength": "1000", "PRIME-SerialNumber": "A18B203008", "445 AO-Volts": "0.0000", "PRIME-X-dimension": "1200", "PRIME-ShutterCloseDelay": "0", "XLIGHT Disk slider-State": "2", "Position": "Default", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 AO-IOChannel": "Dev1/ao5", "520 AO-IOChannel": "Dev1/ao4", "528 AO-TriggerSequenceLength": "1000", "405 Shutter-MaxVolts": "5.0000", "470-DA Device": "470 Shutter", "PRIME-FTCapable": "No", "Core-ChannelGroup": "Channels", "555 AO-SupportsTriggering": "0", "PRIME-Y-dimension": "1200", "Frame": 0, "555 Shutter-Sequenceable": "1", "PRIME-Timing-ExposureTimeNs": "499998000.0000", "Core-ImageProcessor": "", "528-State": "0", "520 AO-Block voltage": "false", "XLIGHT Excitation Wheel-Label": "Excitation-2", "640 Shutter-Demo": "No", "COM3-StopBits": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "520 Shutter-IOChannel": "Dev1/ao11", "Width": 1200, "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-SupportsTriggering": "0", "640 AO-TriggerSampleRate": "100000", "520-Description": "DA device that is used as a shutter", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "405 Shutter-Sequenceable": "1", "405 Shutter-SupportsTriggering": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-TriggerSampleRate": "100000", "640 Shutter-MaxVolts": "5.0000", "555 AO-MaxVolts": "5.0000", "520 AO-TriggerSequenceLength": "1000", "COM3-BaudRate": "9600", "COM9-BaudRate": "9600", "Vincent-D3-Channel #": "Ch. #1", "PRIME-PMode": "Normal", "OpticalPath-Label": "Left(100)", "BitDepth": 16, "555 AO-TriggerSequenceLength": "1000", "445 Shutter-Description": "NI DAC", "405 AO-Sequenceable": "1", "PRIME-AcquisitionMethod": "Callbacks", "555 AO-Name": "AnalogIO", "PRIME-ShutterMode": "Pre-Exposure", "COM3-Handshaking": "Off", "445 AO-SupportsTriggering": "0", "ROI": "0-0-1200-1200", "COM9-Verbose": "1", "PRIME-Color": "OFF", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "PRIME-ShutterOpenDelay": "0", "COM1-Parity": "None", "Slice": 2, "SlicePosition": -30, "COM9-DelayBetweenCharsMs": "0.0000", "555-Name": "DA Shutter", "PIEZO-LudlSingleAxisName": "B", "XLIGHT Emission Wheel-Label": "Empty", "640 Shutter-Name": "AnalogIO", "640 AO-Sequenceable": "1", "520 Shutter-Demo": "No", "528 Shutter-Demo": "No", "470-Name": "DA Shutter", "520-DA Device": "520 Shutter", "XYStage-Description": "Ludl XY stage driver adapter", "445 Shutter-MinVolts": "0.0000", "528 AO-TriggerSampleRate": "100000", "405 AO-MinVolts": "0.0000", "405 Shutter-TriggerSequenceLength": "1000", "Height": 1200, "555 AO-Block voltage": "false", "640 Shutter-Volts": "0.0000", "PixelType": "GRAY16", "445 Shutter-Block voltage": "false", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "520 Shutter-TriggerSequenceLength": "1000", "COM9-Name": "COM9", "Core-SLM": "", "555 AO-Volts": "0.0000", "555 Shutter-Block voltage": "false", "XYStage-Speed": "1800.0000", "520 AO-MaxVolts": "5.0000", "528 Shutter-SupportsTriggering": "0", "COM1-Description": "Serial port driver (boost:asio)", "PRIME-MetadataEnabled": "No", "520 Shutter-MinVolts": "0.0000", "470 Shutter-Sequenceable": "1", "PRIME-PreampDelay": "0", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-MinVolts": "0.0000", "ChannelIndex": 4, "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Core-AutoShutter": "1", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "Exposure-ms": 20, "PRIME-PP 2 ENABLED": "Yes", "640 Shutter-TriggerSampleRate": "100000", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "470 Shutter-IOChannel": "Dev1/ao10", "405 AO-Demo": "No", "PixelSizeUm": 1.1, "470 AO-Demo": "No", "470 Shutter-MaxVolts": "5.0000", "Vincent-D3-Name": "Vincent-D3", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FileName": "img_000000000_P-DIC_002.tif", "LudlController-Reset": "Operate", "PRIME-Exposure": "20.00", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "445-Name": "DA Shutter", "555 Shutter-Name": "AnalogIO", "520 Shutter-TriggerSampleRate": "100000", "445 Shutter-IOChannel": "Dev1/ao9", "PRIME-TransposeCorrection": "0", "XYStage-StepSize": "0.1000", "445-Description": "DA device that is used as a shutter", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 AO-IOChannel": "Dev1/ao7", "520 Shutter-Name": "AnalogIO", "COM3-DataBits": "8", "640 AO-Volts": "0.0000", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "555 Shutter-IOChannel": "Dev1/ao13", "WaitInterval": null, "520 Shutter-Sequenceable": "1", "YPositionUm": 31506.2, "445-State": "0", "445-DA Device": "445 Shutter", "PRIME-CircularBufferAutoSize": "ON", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "555 AO-TriggerSampleRate": "100000", "445 Shutter-Volts": "0.0000", "XLIGHT Hub-Port": "COM3", "Core-AutoFocus": "", "COM4-Handshaking": "Off", "528 Shutter-MinVolts": "0.0000", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "445 Shutter-Name": "AnalogIO", "528 Shutter-Sequenceable": "1", "XYStage-TransposeMirrorX": "0", "XYStage-TransposeMirrorY": "0", "PRIME-ReadoutRate": "100MHz 16bit", "XYStage-Name": "Stage", "640 AO-SupportsTriggering": "0", "640-Name": "DA Shutter", "640 Shutter-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "640 AO-Block voltage": "false", "445 Shutter-Sequenceable": "1", "470 AO-MaxVolts": "5.0000", "COM4-BaudRate": "9600", "470-Description": "DA device that is used as a shutter", "520 Shutter-Description": "NI DAC", "PRIME-CCDTemperature": "-14.8500", "Time": "2019-12-18 21:37:11 -0500", "640 AO-TriggerSequenceLength": "1000", "470 AO-Name": "AnalogIO", "XLIGHT Disk slider-Label": "Disk Pos 40um", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "Binning": "1x1", "TE2000-ModelType": "1", "COM3-AnswerTimeout": "500.0000", "555 Shutter-SupportsTriggering": "0", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "TE2000-Port": "COM4", "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Description": "NI DAC", "COM4-AnswerTimeout": "500.0000", "COM1-DelayBetweenCharsMs": "0.0000", "FilterBlock-Label": "Confocal", "445 AO-Name": "AnalogIO", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-Timing-ReadoutTimeNs": "24672000.0000", "528 AO-Demo": "No", "520 AO-Sequenceable": "1", "555-Description": "DA device that is used as a shutter", "405-DA Device": "405 Shutter", "405 AO-TriggerSequenceLength": "1000", "640 AO-MinVolts": "0.0000", "640 Shutter-Block voltage": "false", "FrameIndex": 0, "PRIME-Port": "CMOS", "555 AO-MinVolts": "0.0000", "COM3-Description": "Serial port driver (boost:asio)", "528 AO-Description": "NI DAC", "555 AO-IOChannel": "Dev1/ao6", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "555 AO-TriggerInputLine": "/Dev1/PFI0", "PRIME-ActualInterval-ms": "390.5200", "LudlController-Port": "COM9", "445 Shutter-Demo": "No", "PRIME-Trigger-Expose Out-Mux": "1", "SliceIndex": 2, "PRIME-CCDTemperatureSetPoint": "-15.0000", "405 Shutter-MinVolts": "0.0000", "PRIME-ExposeOutMode": "First Row", "AxisPositions": null, "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Volts": "0.0000", "528-Name": "DA Shutter", "555-State": "0", "XLIGHT Dichroic-Label": "Di03-R635-t1", "445 AO-Sequenceable": "1", "405-Name": "DA Shutter", "405 AO-IOChannel": "Dev1/ao0", "Core-Camera": "PRIME", "Focus-Name": "Focus", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "405 Shutter-IOChannel": "Dev1/ao8", "640 AO-Demo": "No", "640 Shutter-SupportsTriggering": "0", "445 AO-Demo": "No", "555-DA Device": "555 Shutter", "470 Shutter-Description": "NI DAC", "528 AO-Volts": "0.0000", "COM1-DataBits": "8", "Vincent-D3-Address": "x", "520 AO-SupportsTriggering": "0", "405-Description": "DA device that is used as a shutter", "640 Shutter-MinVolts": "0.0000", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "405 AO-Name": "AnalogIO", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "640 AO-TriggerInputLine": "/Dev1/PFI0", "405 AO-TriggerInputLine": "/Dev1/PFI0", "640-Description": "DA device that is used as a shutter", "445 Shutter-TriggerSampleRate": "100000", "470 Shutter-TriggerSequenceLength": "1000", "COM1-StopBits": "1", "520 Shutter-SupportsTriggering": "0", "405-State": "0", "Core-Initialize": "1", "XLIGHT Emission Wheel-State": "3", "520 AO-Description": "NI DAC", "528 AO-TriggerInputLine": "/Dev1/PFI0", "445 Shutter-MaxVolts": "5.0000", "COM9-Handshaking": "Off", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "XLIGHT Spin motor-Label": "On (spinning)", "PRIME-PP 5 ENABLED": "No", "UUID": "ec25d73a-f368-4d74-984e-d26f9bcdbb88", "PRIME-TriggerMode": "Internal Trigger", "COM1-Handshaking": "Off", "PRIME-Timing-PostTriggerDelayNs": "49344000.0000", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "Focus-Position": "17741.5000", "528 Shutter-Block voltage": "false", "Channel": "P-DIC", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "555 Shutter-Demo": "No", "405 Shutter-Demo": "No", "528 AO-Name": "AnalogIO", "520-State": "0", "Core-Galvo": "", "405 Shutter-TriggerSampleRate": "100000", "Focus-FocusStepSize_(nm)": "100", "PRIME-PixelType": "16bit", "COM4-DataBits": "8", "470 AO-IOChannel": "Dev1/ao2", "520 AO-MinVolts": "0.0000", "COM9-Parity": "None", "PRIME-CircularBufferEnabled": "ON", "PRIME-PP 6 Reset": "No", "640 AO-Description": "NI DAC", "Source": "PRIME", "445 AO-MinVolts": "0.0000", "PIEZO-Name": "Stage", "445 AO-Description": "NI DAC", "PRIME-CircularBufferFrameCount": "46", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "520 AO-Volts": "0.0000", "528 Shutter-TriggerSampleRate": "100000", "XPositionUm": 19731.9, "OpticalPath-State": "4", "PRIME-PP 3 THRESHOLD": "80", "PRIME-PVCAM Version": "3.7.1", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Gain": "1-HDR", "640 Shutter-TriggerSequenceLength": "1000", "528 AO-SupportsTriggering": "0", "445 AO-TriggerInputLine": "/Dev1/PFI0", "OpticalPath-Name": "OpticalPath", "PRIME-PP 4 ENABLED": "Yes", "PRIME-PVCAM Adapter Version": "1.3.29", "COM3-Name": "COM3", "405 AO-MaxVolts": "5.0000", "520 Shutter-Block voltage": "false", "PRIME-Timing-ClearingTimeNs": "49344000.0000", "Core-Focus": "PIEZO", "528 AO-Block voltage": "false", "TE2000-Description": "Nikon TE2000 hub", "528 AO-MinVolts": "0.0000", "COM4-Parity": "None", "Summary": { "Slices": 11, "ChContrastMin": [ 0, 0, 0, 0, 0, 0 ], "PixelAspect": 1, "SlicesFirst": true, "Interval_ms": 0, "Time": "2019-12-18 21:36:42 -0500", "Prefix": "seq_4", "InitialPositionList": [ { "Label": "Pos0", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.21], "Focus": [17741.5], "XYStage": [ 44542.200000000004, 31636.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos1", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.22], "Focus": [17741.4], "XYStage": [ 43801.100000000006, 31637.9 ] }, "GridColumnIndex": 0 }, { "Label": "Pos2", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.5], "XYStage": [ 38561.3, 32040.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos3", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.185], "Focus": [17741.3], "XYStage": [ 37698.4, 32041.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos4", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.16], "Focus": [17741.5], "XYStage": [ 32375.4, 31758.9 ] }, "GridColumnIndex": 0 }, { "Label": "Pos5", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.17000000000002], "Focus": [17741.3], "XYStage": [ 31770.7, 31758.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos6", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.4], "XYStage": [ 26536.800000000003, 31068.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos7", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.205], "Focus": [17741.4], "XYStage": [ 25420.2, 31068.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos8", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.85], "Focus": [17741.3], "XYStage": [ 21059, 31508 ] }, "GridColumnIndex": 0 }, { "Label": "Pos9", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.82], "Focus": [17741.3], "XYStage": [ 19731.9, 31506.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos10", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.79], "Focus": [17741.4], "XYStage": [ 14528.5, 32007.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos11", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.83], "Focus": [17741.2], "XYStage": [ 13830.300000000001, 32009.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos12", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.79], "Focus": [17741.3], "XYStage": [ 8775.300000000001, 32017.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos13", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.815], "Focus": [17741.3], "XYStage": [ 8109.200000000001, 32008.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos14", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.805], "Focus": [17741.3], "XYStage": [ 3483.3, 31908.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos15", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.82], "Focus": [17741.2], "XYStage": [ 2686.8, 31903.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos16", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [211.48000000000002], "Focus": [17741.1], "XYStage": [ -1978.9, 31973.600000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos17", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [211.51500000000001], "Focus": [17741.1], "XYStage": [ -2957.8, 31982.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos18", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.195], "Focus": [17741.5], "XYStage": [ 47747.4, -576.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos19", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.23000000000002], "Focus": [17741.6], "XYStage": [ 47053.9, -576.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos20", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.205], "Focus": [17741.4], "XYStage": [ 41871.200000000004, -479.8 ] }, "GridColumnIndex": 0 }, { "Label": "Pos21", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.4], "XYStage": [ 40882.600000000006, -392 ] }, "GridColumnIndex": 0 }, { "Label": "Pos22", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.24], "Focus": [17741.4], "XYStage": [ 35905.6, -135.3 ] }, "GridColumnIndex": 0 }, { "Label": "Pos23", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.195], "Focus": [17741.5], "XYStage": [ 34990.9, -132.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos24", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.46], "Focus": [17741.4], "XYStage": [ 29678.100000000002, -958.1 ] }, "GridColumnIndex": 0 }, { "Label": "Pos25", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.44], "Focus": [17741.5], "XYStage": [ 28983.600000000002, -939.4000000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos26", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.475], "Focus": [17741.5], "XYStage": [ 23019.7, -122 ] }, "GridColumnIndex": 0 }, { "Label": "Pos27", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.44], "Focus": [17741.7], "XYStage": [ 22428, -116.80000000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos28", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.46], "Focus": [17741.7], "XYStage": [ 16364.900000000001, -181.10000000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos29", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.48000000000002], "Focus": [17741.5], "XYStage": [ 15712.1, -180 ] }, "GridColumnIndex": 0 }, { "Label": "Pos30", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.45000000000002], "Focus": [17741.7], "XYStage": [ 10346.1, 77.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos31", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.49], "Focus": [17741.4], "XYStage": [ 9755.5, 85.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos32", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.475], "Focus": [17741.5], "XYStage": [ 4775.5, 293.90000000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos33", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.445], "Focus": [17741.4], "XYStage": [ 3954.9, 293.90000000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos34", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.455], "Focus": [17741.4], "XYStage": [ -1324.9, 545.6 ] }, "GridColumnIndex": 0 }, { "Label": "Pos35", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.445], "Focus": [17741.3], "XYStage": [ -2097, 576.6 ] }, "GridColumnIndex": 0 } ], "z-step_um": 10, "GridRow": 0, "BitDepth": 16, "Depth": 2, "Channels": 6, "Source": "Micro-Manager", "ChColors": [ -16711681, -256, -65281, -1, -1, -16776961 ], "MetadataVersion": 10, "KeepShutterOpenChannels": true, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536, 65536 ], "CustomIntervals_ms": [], "Height": 1200, "MicroManagerVersion": "1.4.23 20180219", "GridColumn": 0, "UUID": "14a13bf7-c944-4672-b873-fcfbbb7ea13d", "PixelType": "GRAY16", "Width": 1200, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\Yan\\20191218-YC113-S7S8-miseq", "Frames": 1, "Comment": "", "UserName": "Nikon", "PixelSize_um": 1.1, "ROI": [ 0, 0, 1200, 1200 ], "Date": "2019-12-18", "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC", "P-DAPI-high" ], "KeepShutterOpenSlices": true, "IJType": 1, "ComputerName": "ZadorConfocal2", "Positions": 36, "PositionIndex": 9, "CameraTimeout": 20000, "TimeFirst": false }, "640 AO-Name": "AnalogIO", "Vincent-D3-Command": "Close" }, "FrameKey-0-4-3": { "COM4-StopBits": "1", "528 AO-MaxVolts": "5.0000", "XLIGHT Excitation Wheel-State": "1", "470 Shutter-Name": "AnalogIO", "Core-Shutter": "Vincent-D3", "555 AO-Demo": "No", "XLIGHT Dichroic-State": "2", "XYStage-StepSize-Y": "0.1000", "ZPositionUm": 228.82, "XYStage-StepSize-X": "0.1000", "PRIME-FirmwareVersion": "20.28", "PRIME-ClearCycles": "2", "520-Name": "DA Shutter", "405 Shutter-Name": "AnalogIO", "PIEZO-StepSize": "0.0050", "PRIME-ClearMode": "Pre-Exposure", "470 AO-MinVolts": "0.0000", "405 AO-SupportsTriggering": "1", "CameraChannelIndex": 0, "XYStage-StartSpeed": "500.0000", "COM9-StopBits": "2", "405 AO-Description": "NI DAC", "COM4-DelayBetweenCharsMs": "0.0000", "445 AO-Block voltage": "false", "520 AO-TriggerInputLine": "/Dev1/PFI0", "555 AO-Description": "NI DAC", "520 AO-TriggerSampleRate": "100000", "COM4-Verbose": "1", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM4-Name": "COM4", "405 Shutter-Volts": "0.0000", "Vincent-D3-Port": "COM1", "PRIME-PP 4 MIN ADU AFFECTED": "200", "470 Shutter-MinVolts": "0.0000", "PRIME-PP 3 ENABLED": "Yes", "470 AO-Description": "NI DAC", "PRIME-Description": "PVCAM API device adapter", "520 Shutter-MaxVolts": "5.0000", "520 Shutter-Volts": "0.0000", "528 AO-Sequenceable": "1", "PRIME-Binning": "1x1", "555 Shutter-MaxVolts": "5.0000", "528 Shutter-TriggerSequenceLength": "1000", "405 Shutter-Block voltage": "false", "XLIGHT Spin motor-State": "1", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "Vincent-D3-Time to open (ms)": "35.0000", "Vincent-D3-Time to close (ms)": "35.0000", "445 AO-TriggerSampleRate": "100000", "640 Shutter-IOChannel": "Dev1/ao14", "555 AO-Sequenceable": "1", "520 AO-Name": "AnalogIO", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "470 AO-Block voltage": "false", "COM1-Verbose": "1", "COM9-AnswerTimeout": "10000.0000", "528 Shutter-Name": "AnalogIO", "PRIME-Trigger Timeout (secs)": "10", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "NextFrame": 0, "470 AO-TriggerSequenceLength": "1000", "PRIME-TransposeXY": "0", "445 AO-TriggerSequenceLength": "1000", "COM1-Name": "COM1", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "COM9-DataBits": "8", "COM1-AnswerTimeout": "500.0000", "Camera": "", "TE2000-Version": "V2.50-V2.50-V2.10", "528 Shutter-Volts": "0.0000", "XLIGHT Hub-Name": "XLIGHT Hub", "PIEZO-Description": "Ludl stage driver adapter", "PositionName": "Pos9", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSampleRate": "100000", "PRIME-FanSpeedSetpoint": "High", "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "405 AO-TriggerSampleRate": "100000", "470 Shutter-Block voltage": "false", "470-State": "0", "COM3-Parity": "None", "405 AO-Volts": "0.0000", "PRIME-TransposeMirrorY": "0", "PRIME-ChipName": "GS144BSI", "470 AO-Volts": "0.0000", "PRIME-TransposeMirrorX": "0", "445 Shutter-SupportsTriggering": "0", "470 Shutter-SupportsTriggering": "0", "XLIGHT Hub-Description": "XLIGHT hub", "PRIME-PreampOffLimit": "10000", "470 AO-Sequenceable": "1", "528 Shutter-MaxVolts": "5.0000", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "Core-XYStage": "XYStage", "470 AO-TriggerSampleRate": "100000", "PRIME-Timing-PreTriggerDelayNs": "24672000.0000", "528-DA Device": "528 Shutter", "Focus-Description": "Nikon TE2000 focus adapter", "FilterBlock-State": "5", "520 AO-Demo": "No", "COM3-DelayBetweenCharsMs": "0.0000", "470 Shutter-Volts": "0.0000", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "PRIME-PP 2 THRESHOLD": "125", "PRIME-PP 4 THRESHOLD": "75", "XYStage-Acceleration": "4.0000", "470 Shutter-Demo": "No", "PRIME-Name": "PMPCIECam00", "640-DA Device": "640 Shutter", "405 AO-Block voltage": "false", "ElapsedTime-ms": 403549, "PRIME-Offset": "100", "445 AO-IOChannel": "Dev1/ao1", "640 AO-MaxVolts": "5.0000", "PositionIndex": 9, "PRIME-PP 1 ENABLED": "Yes", "COM1-BaudRate": "9600", "640-State": "0", "COM3-Verbose": "1", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "LudlController-TransmissionDelay": "0", "PRIME-FullWellCapacity": "62000", "PRIME-SMARTStreamingEnabled": "No", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "640 Shutter-Description": "NI DAC", "528-Description": "DA device that is used as a shutter", "COM4-Description": "Serial port driver (boost:asio)", "405 Shutter-Description": "NI DAC", "TE2000-Name": "TE2000", "528 Shutter-Description": "NI DAC", "COM9-Description": "Serial port driver (boost:asio)", "LudlController-Name": "LudlController", "445 Shutter-TriggerSequenceLength": "1000", "PRIME-SerialNumber": "A18B203008", "445 AO-Volts": "0.0000", "PRIME-X-dimension": "1200", "PRIME-ShutterCloseDelay": "0", "XLIGHT Disk slider-State": "2", "Position": "Default", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 AO-IOChannel": "Dev1/ao5", "520 AO-IOChannel": "Dev1/ao4", "528 AO-TriggerSequenceLength": "1000", "405 Shutter-MaxVolts": "5.0000", "470-DA Device": "470 Shutter", "PRIME-FTCapable": "No", "Core-ChannelGroup": "Channels", "555 AO-SupportsTriggering": "0", "PRIME-Y-dimension": "1200", "Frame": 0, "555 Shutter-Sequenceable": "1", "PRIME-Timing-ExposureTimeNs": "499998000.0000", "Core-ImageProcessor": "", "528-State": "0", "520 AO-Block voltage": "false", "XLIGHT Excitation Wheel-Label": "Excitation-2", "640 Shutter-Demo": "No", "COM3-StopBits": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "520 Shutter-IOChannel": "Dev1/ao11", "Width": 1200, "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-SupportsTriggering": "0", "640 AO-TriggerSampleRate": "100000", "520-Description": "DA device that is used as a shutter", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "405 Shutter-Sequenceable": "1", "405 Shutter-SupportsTriggering": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-TriggerSampleRate": "100000", "640 Shutter-MaxVolts": "5.0000", "555 AO-MaxVolts": "5.0000", "520 AO-TriggerSequenceLength": "1000", "COM3-BaudRate": "9600", "COM9-BaudRate": "9600", "Vincent-D3-Channel #": "Ch. #1", "PRIME-PMode": "Normal", "OpticalPath-Label": "Left(100)", "BitDepth": 16, "555 AO-TriggerSequenceLength": "1000", "445 Shutter-Description": "NI DAC", "405 AO-Sequenceable": "1", "PRIME-AcquisitionMethod": "Callbacks", "555 AO-Name": "AnalogIO", "PRIME-ShutterMode": "Pre-Exposure", "COM3-Handshaking": "Off", "445 AO-SupportsTriggering": "0", "ROI": "0-0-1200-1200", "COM9-Verbose": "1", "PRIME-Color": "OFF", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "PRIME-ShutterOpenDelay": "0", "COM1-Parity": "None", "Slice": 3, "SlicePosition": -20, "COM9-DelayBetweenCharsMs": "0.0000", "555-Name": "DA Shutter", "PIEZO-LudlSingleAxisName": "B", "XLIGHT Emission Wheel-Label": "Empty", "640 Shutter-Name": "AnalogIO", "640 AO-Sequenceable": "1", "520 Shutter-Demo": "No", "528 Shutter-Demo": "No", "470-Name": "DA Shutter", "520-DA Device": "520 Shutter", "XYStage-Description": "Ludl XY stage driver adapter", "445 Shutter-MinVolts": "0.0000", "528 AO-TriggerSampleRate": "100000", "405 AO-MinVolts": "0.0000", "405 Shutter-TriggerSequenceLength": "1000", "Height": 1200, "555 AO-Block voltage": "false", "640 Shutter-Volts": "0.0000", "PixelType": "GRAY16", "445 Shutter-Block voltage": "false", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "520 Shutter-TriggerSequenceLength": "1000", "COM9-Name": "COM9", "Core-SLM": "", "555 AO-Volts": "0.0000", "555 Shutter-Block voltage": "false", "XYStage-Speed": "1800.0000", "520 AO-MaxVolts": "5.0000", "528 Shutter-SupportsTriggering": "0", "COM1-Description": "Serial port driver (boost:asio)", "PRIME-MetadataEnabled": "No", "520 Shutter-MinVolts": "0.0000", "470 Shutter-Sequenceable": "1", "PRIME-PreampDelay": "0", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-MinVolts": "0.0000", "ChannelIndex": 4, "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Core-AutoShutter": "1", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "Exposure-ms": 20, "PRIME-PP 2 ENABLED": "Yes", "640 Shutter-TriggerSampleRate": "100000", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "470 Shutter-IOChannel": "Dev1/ao10", "405 AO-Demo": "No", "PixelSizeUm": 1.1, "470 AO-Demo": "No", "470 Shutter-MaxVolts": "5.0000", "Vincent-D3-Name": "Vincent-D3", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FileName": "img_000000000_P-DIC_003.tif", "LudlController-Reset": "Operate", "PRIME-Exposure": "20.00", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "445-Name": "DA Shutter", "555 Shutter-Name": "AnalogIO", "520 Shutter-TriggerSampleRate": "100000", "445 Shutter-IOChannel": "Dev1/ao9", "PRIME-TransposeCorrection": "0", "XYStage-StepSize": "0.1000", "445-Description": "DA device that is used as a shutter", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 AO-IOChannel": "Dev1/ao7", "520 Shutter-Name": "AnalogIO", "COM3-DataBits": "8", "640 AO-Volts": "0.0000", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "555 Shutter-IOChannel": "Dev1/ao13", "WaitInterval": null, "520 Shutter-Sequenceable": "1", "YPositionUm": 31506.2, "445-State": "0", "445-DA Device": "445 Shutter", "PRIME-CircularBufferAutoSize": "ON", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "555 AO-TriggerSampleRate": "100000", "445 Shutter-Volts": "0.0000", "XLIGHT Hub-Port": "COM3", "Core-AutoFocus": "", "COM4-Handshaking": "Off", "528 Shutter-MinVolts": "0.0000", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "445 Shutter-Name": "AnalogIO", "528 Shutter-Sequenceable": "1", "XYStage-TransposeMirrorX": "0", "XYStage-TransposeMirrorY": "0", "PRIME-ReadoutRate": "100MHz 16bit", "XYStage-Name": "Stage", "640 AO-SupportsTriggering": "0", "640-Name": "DA Shutter", "640 Shutter-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "640 AO-Block voltage": "false", "445 Shutter-Sequenceable": "1", "470 AO-MaxVolts": "5.0000", "COM4-BaudRate": "9600", "470-Description": "DA device that is used as a shutter", "520 Shutter-Description": "NI DAC", "PRIME-CCDTemperature": "-14.8500", "Time": "2019-12-18 21:37:11 -0500", "640 AO-TriggerSequenceLength": "1000", "470 AO-Name": "AnalogIO", "XLIGHT Disk slider-Label": "Disk Pos 40um", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "Binning": "1x1", "TE2000-ModelType": "1", "COM3-AnswerTimeout": "500.0000", "555 Shutter-SupportsTriggering": "0", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "TE2000-Port": "COM4", "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Description": "NI DAC", "COM4-AnswerTimeout": "500.0000", "COM1-DelayBetweenCharsMs": "0.0000", "FilterBlock-Label": "Confocal", "445 AO-Name": "AnalogIO", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-Timing-ReadoutTimeNs": "24672000.0000", "528 AO-Demo": "No", "520 AO-Sequenceable": "1", "555-Description": "DA device that is used as a shutter", "405-DA Device": "405 Shutter", "405 AO-TriggerSequenceLength": "1000", "640 AO-MinVolts": "0.0000", "640 Shutter-Block voltage": "false", "FrameIndex": 0, "PRIME-Port": "CMOS", "555 AO-MinVolts": "0.0000", "COM3-Description": "Serial port driver (boost:asio)", "528 AO-Description": "NI DAC", "555 AO-IOChannel": "Dev1/ao6", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "555 AO-TriggerInputLine": "/Dev1/PFI0", "PRIME-ActualInterval-ms": "390.5200", "LudlController-Port": "COM9", "445 Shutter-Demo": "No", "PRIME-Trigger-Expose Out-Mux": "1", "SliceIndex": 3, "PRIME-CCDTemperatureSetPoint": "-15.0000", "405 Shutter-MinVolts": "0.0000", "PRIME-ExposeOutMode": "First Row", "AxisPositions": null, "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Volts": "0.0000", "528-Name": "DA Shutter", "555-State": "0", "XLIGHT Dichroic-Label": "Di03-R635-t1", "445 AO-Sequenceable": "1", "405-Name": "DA Shutter", "405 AO-IOChannel": "Dev1/ao0", "Core-Camera": "PRIME", "Focus-Name": "Focus", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "405 Shutter-IOChannel": "Dev1/ao8", "640 AO-Demo": "No", "640 Shutter-SupportsTriggering": "0", "445 AO-Demo": "No", "555-DA Device": "555 Shutter", "470 Shutter-Description": "NI DAC", "528 AO-Volts": "0.0000", "COM1-DataBits": "8", "Vincent-D3-Address": "x", "520 AO-SupportsTriggering": "0", "405-Description": "DA device that is used as a shutter", "640 Shutter-MinVolts": "0.0000", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "405 AO-Name": "AnalogIO", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "640 AO-TriggerInputLine": "/Dev1/PFI0", "405 AO-TriggerInputLine": "/Dev1/PFI0", "640-Description": "DA device that is used as a shutter", "445 Shutter-TriggerSampleRate": "100000", "470 Shutter-TriggerSequenceLength": "1000", "COM1-StopBits": "1", "520 Shutter-SupportsTriggering": "0", "405-State": "0", "Core-Initialize": "1", "XLIGHT Emission Wheel-State": "3", "520 AO-Description": "NI DAC", "528 AO-TriggerInputLine": "/Dev1/PFI0", "445 Shutter-MaxVolts": "5.0000", "COM9-Handshaking": "Off", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "XLIGHT Spin motor-Label": "On (spinning)", "PRIME-PP 5 ENABLED": "No", "UUID": "a191d539-66fd-4c39-8614-f5abeb078577", "PRIME-TriggerMode": "Internal Trigger", "COM1-Handshaking": "Off", "PRIME-Timing-PostTriggerDelayNs": "49344000.0000", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "Focus-Position": "17741.5000", "528 Shutter-Block voltage": "false", "Channel": "P-DIC", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "555 Shutter-Demo": "No", "405 Shutter-Demo": "No", "528 AO-Name": "AnalogIO", "520-State": "0", "Core-Galvo": "", "405 Shutter-TriggerSampleRate": "100000", "Focus-FocusStepSize_(nm)": "100", "PRIME-PixelType": "16bit", "COM4-DataBits": "8", "470 AO-IOChannel": "Dev1/ao2", "520 AO-MinVolts": "0.0000", "COM9-Parity": "None", "PRIME-CircularBufferEnabled": "ON", "PRIME-PP 6 Reset": "No", "640 AO-Description": "NI DAC", "Source": "PRIME", "445 AO-MinVolts": "0.0000", "PIEZO-Name": "Stage", "445 AO-Description": "NI DAC", "PRIME-CircularBufferFrameCount": "46", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "520 AO-Volts": "0.0000", "528 Shutter-TriggerSampleRate": "100000", "XPositionUm": 19731.9, "OpticalPath-State": "4", "PRIME-PP 3 THRESHOLD": "80", "PRIME-PVCAM Version": "3.7.1", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Gain": "1-HDR", "640 Shutter-TriggerSequenceLength": "1000", "528 AO-SupportsTriggering": "0", "445 AO-TriggerInputLine": "/Dev1/PFI0", "OpticalPath-Name": "OpticalPath", "PRIME-PP 4 ENABLED": "Yes", "PRIME-PVCAM Adapter Version": "1.3.29", "COM3-Name": "COM3", "405 AO-MaxVolts": "5.0000", "520 Shutter-Block voltage": "false", "PRIME-Timing-ClearingTimeNs": "49344000.0000", "Core-Focus": "PIEZO", "528 AO-Block voltage": "false", "TE2000-Description": "Nikon TE2000 hub", "528 AO-MinVolts": "0.0000", "COM4-Parity": "None", "Summary": { "Slices": 11, "ChContrastMin": [ 0, 0, 0, 0, 0, 0 ], "PixelAspect": 1, "SlicesFirst": true, "Interval_ms": 0, "Time": "2019-12-18 21:36:42 -0500", "Prefix": "seq_4", "InitialPositionList": [ { "Label": "Pos0", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.21], "Focus": [17741.5], "XYStage": [ 44542.200000000004, 31636.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos1", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.22], "Focus": [17741.4], "XYStage": [ 43801.100000000006, 31637.9 ] }, "GridColumnIndex": 0 }, { "Label": "Pos2", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.5], "XYStage": [ 38561.3, 32040.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos3", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.185], "Focus": [17741.3], "XYStage": [ 37698.4, 32041.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos4", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.16], "Focus": [17741.5], "XYStage": [ 32375.4, 31758.9 ] }, "GridColumnIndex": 0 }, { "Label": "Pos5", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.17000000000002], "Focus": [17741.3], "XYStage": [ 31770.7, 31758.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos6", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.4], "XYStage": [ 26536.800000000003, 31068.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos7", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.205], "Focus": [17741.4], "XYStage": [ 25420.2, 31068.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos8", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.85], "Focus": [17741.3], "XYStage": [ 21059, 31508 ] }, "GridColumnIndex": 0 }, { "Label": "Pos9", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.82], "Focus": [17741.3], "XYStage": [ 19731.9, 31506.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos10", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.79], "Focus": [17741.4], "XYStage": [ 14528.5, 32007.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos11", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.83], "Focus": [17741.2], "XYStage": [ 13830.300000000001, 32009.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos12", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.79], "Focus": [17741.3], "XYStage": [ 8775.300000000001, 32017.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos13", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.815], "Focus": [17741.3], "XYStage": [ 8109.200000000001, 32008.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos14", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.805], "Focus": [17741.3], "XYStage": [ 3483.3, 31908.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos15", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.82], "Focus": [17741.2], "XYStage": [ 2686.8, 31903.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos16", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [211.48000000000002], "Focus": [17741.1], "XYStage": [ -1978.9, 31973.600000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos17", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [211.51500000000001], "Focus": [17741.1], "XYStage": [ -2957.8, 31982.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos18", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.195], "Focus": [17741.5], "XYStage": [ 47747.4, -576.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos19", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.23000000000002], "Focus": [17741.6], "XYStage": [ 47053.9, -576.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos20", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.205], "Focus": [17741.4], "XYStage": [ 41871.200000000004, -479.8 ] }, "GridColumnIndex": 0 }, { "Label": "Pos21", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.4], "XYStage": [ 40882.600000000006, -392 ] }, "GridColumnIndex": 0 }, { "Label": "Pos22", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.24], "Focus": [17741.4], "XYStage": [ 35905.6, -135.3 ] }, "GridColumnIndex": 0 }, { "Label": "Pos23", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.195], "Focus": [17741.5], "XYStage": [ 34990.9, -132.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos24", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.46], "Focus": [17741.4], "XYStage": [ 29678.100000000002, -958.1 ] }, "GridColumnIndex": 0 }, { "Label": "Pos25", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.44], "Focus": [17741.5], "XYStage": [ 28983.600000000002, -939.4000000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos26", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.475], "Focus": [17741.5], "XYStage": [ 23019.7, -122 ] }, "GridColumnIndex": 0 }, { "Label": "Pos27", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.44], "Focus": [17741.7], "XYStage": [ 22428, -116.80000000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos28", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.46], "Focus": [17741.7], "XYStage": [ 16364.900000000001, -181.10000000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos29", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.48000000000002], "Focus": [17741.5], "XYStage": [ 15712.1, -180 ] }, "GridColumnIndex": 0 }, { "Label": "Pos30", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.45000000000002], "Focus": [17741.7], "XYStage": [ 10346.1, 77.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos31", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.49], "Focus": [17741.4], "XYStage": [ 9755.5, 85.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos32", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.475], "Focus": [17741.5], "XYStage": [ 4775.5, 293.90000000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos33", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.445], "Focus": [17741.4], "XYStage": [ 3954.9, 293.90000000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos34", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.455], "Focus": [17741.4], "XYStage": [ -1324.9, 545.6 ] }, "GridColumnIndex": 0 }, { "Label": "Pos35", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.445], "Focus": [17741.3], "XYStage": [ -2097, 576.6 ] }, "GridColumnIndex": 0 } ], "z-step_um": 10, "GridRow": 0, "BitDepth": 16, "Depth": 2, "Channels": 6, "Source": "Micro-Manager", "ChColors": [ -16711681, -256, -65281, -1, -1, -16776961 ], "MetadataVersion": 10, "KeepShutterOpenChannels": true, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536, 65536 ], "CustomIntervals_ms": [], "Height": 1200, "MicroManagerVersion": "1.4.23 20180219", "GridColumn": 0, "UUID": "14a13bf7-c944-4672-b873-fcfbbb7ea13d", "PixelType": "GRAY16", "Width": 1200, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\Yan\\20191218-YC113-S7S8-miseq", "Frames": 1, "Comment": "", "UserName": "Nikon", "PixelSize_um": 1.1, "ROI": [ 0, 0, 1200, 1200 ], "Date": "2019-12-18", "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC", "P-DAPI-high" ], "KeepShutterOpenSlices": true, "IJType": 1, "ComputerName": "ZadorConfocal2", "Positions": 36, "PositionIndex": 9, "CameraTimeout": 20000, "TimeFirst": false }, "640 AO-Name": "AnalogIO", "Vincent-D3-Command": "Close" }, "FrameKey-0-4-4": { "COM4-StopBits": "1", "528 AO-MaxVolts": "5.0000", "XLIGHT Excitation Wheel-State": "1", "470 Shutter-Name": "AnalogIO", "Core-Shutter": "Vincent-D3", "555 AO-Demo": "No", "XLIGHT Dichroic-State": "2", "XYStage-StepSize-Y": "0.1000", "ZPositionUm": 238.82, "XYStage-StepSize-X": "0.1000", "PRIME-FirmwareVersion": "20.28", "PRIME-ClearCycles": "2", "520-Name": "DA Shutter", "405 Shutter-Name": "AnalogIO", "PIEZO-StepSize": "0.0050", "PRIME-ClearMode": "Pre-Exposure", "470 AO-MinVolts": "0.0000", "405 AO-SupportsTriggering": "1", "CameraChannelIndex": 0, "XYStage-StartSpeed": "500.0000", "COM9-StopBits": "2", "405 AO-Description": "NI DAC", "COM4-DelayBetweenCharsMs": "0.0000", "445 AO-Block voltage": "false", "520 AO-TriggerInputLine": "/Dev1/PFI0", "555 AO-Description": "NI DAC", "520 AO-TriggerSampleRate": "100000", "COM4-Verbose": "1", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM4-Name": "COM4", "405 Shutter-Volts": "0.0000", "Vincent-D3-Port": "COM1", "PRIME-PP 4 MIN ADU AFFECTED": "200", "470 Shutter-MinVolts": "0.0000", "PRIME-PP 3 ENABLED": "Yes", "470 AO-Description": "NI DAC", "PRIME-Description": "PVCAM API device adapter", "520 Shutter-MaxVolts": "5.0000", "520 Shutter-Volts": "0.0000", "528 AO-Sequenceable": "1", "PRIME-Binning": "1x1", "555 Shutter-MaxVolts": "5.0000", "528 Shutter-TriggerSequenceLength": "1000", "405 Shutter-Block voltage": "false", "XLIGHT Spin motor-State": "1", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "Vincent-D3-Time to open (ms)": "35.0000", "Vincent-D3-Time to close (ms)": "35.0000", "445 AO-TriggerSampleRate": "100000", "640 Shutter-IOChannel": "Dev1/ao14", "555 AO-Sequenceable": "1", "520 AO-Name": "AnalogIO", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "470 AO-Block voltage": "false", "COM1-Verbose": "1", "COM9-AnswerTimeout": "10000.0000", "528 Shutter-Name": "AnalogIO", "PRIME-Trigger Timeout (secs)": "10", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "NextFrame": 0, "470 AO-TriggerSequenceLength": "1000", "PRIME-TransposeXY": "0", "445 AO-TriggerSequenceLength": "1000", "COM1-Name": "COM1", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "COM9-DataBits": "8", "COM1-AnswerTimeout": "500.0000", "Camera": "", "TE2000-Version": "V2.50-V2.50-V2.10", "528 Shutter-Volts": "0.0000", "XLIGHT Hub-Name": "XLIGHT Hub", "PIEZO-Description": "Ludl stage driver adapter", "PositionName": "Pos9", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSampleRate": "100000", "PRIME-FanSpeedSetpoint": "High", "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "405 AO-TriggerSampleRate": "100000", "470 Shutter-Block voltage": "false", "470-State": "0", "COM3-Parity": "None", "405 AO-Volts": "0.0000", "PRIME-TransposeMirrorY": "0", "PRIME-ChipName": "GS144BSI", "470 AO-Volts": "0.0000", "PRIME-TransposeMirrorX": "0", "445 Shutter-SupportsTriggering": "0", "470 Shutter-SupportsTriggering": "0", "XLIGHT Hub-Description": "XLIGHT hub", "PRIME-PreampOffLimit": "10000", "470 AO-Sequenceable": "1", "528 Shutter-MaxVolts": "5.0000", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "Core-XYStage": "XYStage", "470 AO-TriggerSampleRate": "100000", "PRIME-Timing-PreTriggerDelayNs": "24672000.0000", "528-DA Device": "528 Shutter", "Focus-Description": "Nikon TE2000 focus adapter", "FilterBlock-State": "5", "520 AO-Demo": "No", "COM3-DelayBetweenCharsMs": "0.0000", "470 Shutter-Volts": "0.0000", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "PRIME-PP 2 THRESHOLD": "125", "PRIME-PP 4 THRESHOLD": "75", "XYStage-Acceleration": "4.0000", "470 Shutter-Demo": "No", "PRIME-Name": "PMPCIECam00", "640-DA Device": "640 Shutter", "405 AO-Block voltage": "false", "ElapsedTime-ms": 403852, "PRIME-Offset": "100", "445 AO-IOChannel": "Dev1/ao1", "640 AO-MaxVolts": "5.0000", "PositionIndex": 9, "PRIME-PP 1 ENABLED": "Yes", "COM1-BaudRate": "9600", "640-State": "0", "COM3-Verbose": "1", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "LudlController-TransmissionDelay": "0", "PRIME-FullWellCapacity": "62000", "PRIME-SMARTStreamingEnabled": "No", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "640 Shutter-Description": "NI DAC", "528-Description": "DA device that is used as a shutter", "COM4-Description": "Serial port driver (boost:asio)", "405 Shutter-Description": "NI DAC", "TE2000-Name": "TE2000", "528 Shutter-Description": "NI DAC", "COM9-Description": "Serial port driver (boost:asio)", "LudlController-Name": "LudlController", "445 Shutter-TriggerSequenceLength": "1000", "PRIME-SerialNumber": "A18B203008", "445 AO-Volts": "0.0000", "PRIME-X-dimension": "1200", "PRIME-ShutterCloseDelay": "0", "XLIGHT Disk slider-State": "2", "Position": "Default", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 AO-IOChannel": "Dev1/ao5", "520 AO-IOChannel": "Dev1/ao4", "528 AO-TriggerSequenceLength": "1000", "405 Shutter-MaxVolts": "5.0000", "470-DA Device": "470 Shutter", "PRIME-FTCapable": "No", "Core-ChannelGroup": "Channels", "555 AO-SupportsTriggering": "0", "PRIME-Y-dimension": "1200", "Frame": 0, "555 Shutter-Sequenceable": "1", "PRIME-Timing-ExposureTimeNs": "499998000.0000", "Core-ImageProcessor": "", "528-State": "0", "520 AO-Block voltage": "false", "XLIGHT Excitation Wheel-Label": "Excitation-2", "640 Shutter-Demo": "No", "COM3-StopBits": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "520 Shutter-IOChannel": "Dev1/ao11", "Width": 1200, "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-SupportsTriggering": "0", "640 AO-TriggerSampleRate": "100000", "520-Description": "DA device that is used as a shutter", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "405 Shutter-Sequenceable": "1", "405 Shutter-SupportsTriggering": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-TriggerSampleRate": "100000", "640 Shutter-MaxVolts": "5.0000", "555 AO-MaxVolts": "5.0000", "520 AO-TriggerSequenceLength": "1000", "COM3-BaudRate": "9600", "COM9-BaudRate": "9600", "Vincent-D3-Channel #": "Ch. #1", "PRIME-PMode": "Normal", "OpticalPath-Label": "Left(100)", "BitDepth": 16, "555 AO-TriggerSequenceLength": "1000", "445 Shutter-Description": "NI DAC", "405 AO-Sequenceable": "1", "PRIME-AcquisitionMethod": "Callbacks", "555 AO-Name": "AnalogIO", "PRIME-ShutterMode": "Pre-Exposure", "COM3-Handshaking": "Off", "445 AO-SupportsTriggering": "0", "ROI": "0-0-1200-1200", "COM9-Verbose": "1", "PRIME-Color": "OFF", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "PRIME-ShutterOpenDelay": "0", "COM1-Parity": "None", "Slice": 4, "SlicePosition": -10, "COM9-DelayBetweenCharsMs": "0.0000", "555-Name": "DA Shutter", "PIEZO-LudlSingleAxisName": "B", "XLIGHT Emission Wheel-Label": "Empty", "640 Shutter-Name": "AnalogIO", "640 AO-Sequenceable": "1", "520 Shutter-Demo": "No", "528 Shutter-Demo": "No", "470-Name": "DA Shutter", "520-DA Device": "520 Shutter", "XYStage-Description": "Ludl XY stage driver adapter", "445 Shutter-MinVolts": "0.0000", "528 AO-TriggerSampleRate": "100000", "405 AO-MinVolts": "0.0000", "405 Shutter-TriggerSequenceLength": "1000", "Height": 1200, "555 AO-Block voltage": "false", "640 Shutter-Volts": "0.0000", "PixelType": "GRAY16", "445 Shutter-Block voltage": "false", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "520 Shutter-TriggerSequenceLength": "1000", "COM9-Name": "COM9", "Core-SLM": "", "555 AO-Volts": "0.0000", "555 Shutter-Block voltage": "false", "XYStage-Speed": "1800.0000", "520 AO-MaxVolts": "5.0000", "528 Shutter-SupportsTriggering": "0", "COM1-Description": "Serial port driver (boost:asio)", "PRIME-MetadataEnabled": "No", "520 Shutter-MinVolts": "0.0000", "470 Shutter-Sequenceable": "1", "PRIME-PreampDelay": "0", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-MinVolts": "0.0000", "ChannelIndex": 4, "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Core-AutoShutter": "1", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "Exposure-ms": 20, "PRIME-PP 2 ENABLED": "Yes", "640 Shutter-TriggerSampleRate": "100000", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "470 Shutter-IOChannel": "Dev1/ao10", "405 AO-Demo": "No", "PixelSizeUm": 1.1, "470 AO-Demo": "No", "470 Shutter-MaxVolts": "5.0000", "Vincent-D3-Name": "Vincent-D3", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FileName": "img_000000000_P-DIC_004.tif", "LudlController-Reset": "Operate", "PRIME-Exposure": "20.00", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "445-Name": "DA Shutter", "555 Shutter-Name": "AnalogIO", "520 Shutter-TriggerSampleRate": "100000", "445 Shutter-IOChannel": "Dev1/ao9", "PRIME-TransposeCorrection": "0", "XYStage-StepSize": "0.1000", "445-Description": "DA device that is used as a shutter", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 AO-IOChannel": "Dev1/ao7", "520 Shutter-Name": "AnalogIO", "COM3-DataBits": "8", "640 AO-Volts": "0.0000", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "555 Shutter-IOChannel": "Dev1/ao13", "WaitInterval": null, "520 Shutter-Sequenceable": "1", "YPositionUm": 31506.2, "445-State": "0", "445-DA Device": "445 Shutter", "PRIME-CircularBufferAutoSize": "ON", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "555 AO-TriggerSampleRate": "100000", "445 Shutter-Volts": "0.0000", "XLIGHT Hub-Port": "COM3", "Core-AutoFocus": "", "COM4-Handshaking": "Off", "528 Shutter-MinVolts": "0.0000", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "445 Shutter-Name": "AnalogIO", "528 Shutter-Sequenceable": "1", "XYStage-TransposeMirrorX": "0", "XYStage-TransposeMirrorY": "0", "PRIME-ReadoutRate": "100MHz 16bit", "XYStage-Name": "Stage", "640 AO-SupportsTriggering": "0", "640-Name": "DA Shutter", "640 Shutter-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "640 AO-Block voltage": "false", "445 Shutter-Sequenceable": "1", "470 AO-MaxVolts": "5.0000", "COM4-BaudRate": "9600", "470-Description": "DA device that is used as a shutter", "520 Shutter-Description": "NI DAC", "PRIME-CCDTemperature": "-14.8500", "Time": "2019-12-18 21:37:11 -0500", "640 AO-TriggerSequenceLength": "1000", "470 AO-Name": "AnalogIO", "XLIGHT Disk slider-Label": "Disk Pos 40um", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "Binning": "1x1", "TE2000-ModelType": "1", "COM3-AnswerTimeout": "500.0000", "555 Shutter-SupportsTriggering": "0", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "TE2000-Port": "COM4", "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Description": "NI DAC", "COM4-AnswerTimeout": "500.0000", "COM1-DelayBetweenCharsMs": "0.0000", "FilterBlock-Label": "Confocal", "445 AO-Name": "AnalogIO", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-Timing-ReadoutTimeNs": "24672000.0000", "528 AO-Demo": "No", "520 AO-Sequenceable": "1", "555-Description": "DA device that is used as a shutter", "405-DA Device": "405 Shutter", "405 AO-TriggerSequenceLength": "1000", "640 AO-MinVolts": "0.0000", "640 Shutter-Block voltage": "false", "FrameIndex": 0, "PRIME-Port": "CMOS", "555 AO-MinVolts": "0.0000", "COM3-Description": "Serial port driver (boost:asio)", "528 AO-Description": "NI DAC", "555 AO-IOChannel": "Dev1/ao6", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "555 AO-TriggerInputLine": "/Dev1/PFI0", "PRIME-ActualInterval-ms": "390.5200", "LudlController-Port": "COM9", "445 Shutter-Demo": "No", "PRIME-Trigger-Expose Out-Mux": "1", "SliceIndex": 4, "PRIME-CCDTemperatureSetPoint": "-15.0000", "405 Shutter-MinVolts": "0.0000", "PRIME-ExposeOutMode": "First Row", "AxisPositions": null, "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Volts": "0.0000", "528-Name": "DA Shutter", "555-State": "0", "XLIGHT Dichroic-Label": "Di03-R635-t1", "445 AO-Sequenceable": "1", "405-Name": "DA Shutter", "405 AO-IOChannel": "Dev1/ao0", "Core-Camera": "PRIME", "Focus-Name": "Focus", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "405 Shutter-IOChannel": "Dev1/ao8", "640 AO-Demo": "No", "640 Shutter-SupportsTriggering": "0", "445 AO-Demo": "No", "555-DA Device": "555 Shutter", "470 Shutter-Description": "NI DAC", "528 AO-Volts": "0.0000", "COM1-DataBits": "8", "Vincent-D3-Address": "x", "520 AO-SupportsTriggering": "0", "405-Description": "DA device that is used as a shutter", "640 Shutter-MinVolts": "0.0000", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "405 AO-Name": "AnalogIO", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "640 AO-TriggerInputLine": "/Dev1/PFI0", "405 AO-TriggerInputLine": "/Dev1/PFI0", "640-Description": "DA device that is used as a shutter", "445 Shutter-TriggerSampleRate": "100000", "470 Shutter-TriggerSequenceLength": "1000", "COM1-StopBits": "1", "520 Shutter-SupportsTriggering": "0", "405-State": "0", "Core-Initialize": "1", "XLIGHT Emission Wheel-State": "3", "520 AO-Description": "NI DAC", "528 AO-TriggerInputLine": "/Dev1/PFI0", "445 Shutter-MaxVolts": "5.0000", "COM9-Handshaking": "Off", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "XLIGHT Spin motor-Label": "On (spinning)", "PRIME-PP 5 ENABLED": "No", "UUID": "79b94b6c-60b3-4320-b518-fd26f04dba24", "PRIME-TriggerMode": "Internal Trigger", "COM1-Handshaking": "Off", "PRIME-Timing-PostTriggerDelayNs": "49344000.0000", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "Focus-Position": "17741.5000", "528 Shutter-Block voltage": "false", "Channel": "P-DIC", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "555 Shutter-Demo": "No", "405 Shutter-Demo": "No", "528 AO-Name": "AnalogIO", "520-State": "0", "Core-Galvo": "", "405 Shutter-TriggerSampleRate": "100000", "Focus-FocusStepSize_(nm)": "100", "PRIME-PixelType": "16bit", "COM4-DataBits": "8", "470 AO-IOChannel": "Dev1/ao2", "520 AO-MinVolts": "0.0000", "COM9-Parity": "None", "PRIME-CircularBufferEnabled": "ON", "PRIME-PP 6 Reset": "No", "640 AO-Description": "NI DAC", "Source": "PRIME", "445 AO-MinVolts": "0.0000", "PIEZO-Name": "Stage", "445 AO-Description": "NI DAC", "PRIME-CircularBufferFrameCount": "46", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "520 AO-Volts": "0.0000", "528 Shutter-TriggerSampleRate": "100000", "XPositionUm": 19731.9, "OpticalPath-State": "4", "PRIME-PP 3 THRESHOLD": "80", "PRIME-PVCAM Version": "3.7.1", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Gain": "1-HDR", "640 Shutter-TriggerSequenceLength": "1000", "528 AO-SupportsTriggering": "0", "445 AO-TriggerInputLine": "/Dev1/PFI0", "OpticalPath-Name": "OpticalPath", "PRIME-PP 4 ENABLED": "Yes", "PRIME-PVCAM Adapter Version": "1.3.29", "COM3-Name": "COM3", "405 AO-MaxVolts": "5.0000", "520 Shutter-Block voltage": "false", "PRIME-Timing-ClearingTimeNs": "49344000.0000", "Core-Focus": "PIEZO", "528 AO-Block voltage": "false", "TE2000-Description": "Nikon TE2000 hub", "528 AO-MinVolts": "0.0000", "COM4-Parity": "None", "Summary": { "Slices": 11, "ChContrastMin": [ 0, 0, 0, 0, 0, 0 ], "PixelAspect": 1, "SlicesFirst": true, "Interval_ms": 0, "Time": "2019-12-18 21:36:42 -0500", "Prefix": "seq_4", "InitialPositionList": [ { "Label": "Pos0", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.21], "Focus": [17741.5], "XYStage": [ 44542.200000000004, 31636.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos1", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.22], "Focus": [17741.4], "XYStage": [ 43801.100000000006, 31637.9 ] }, "GridColumnIndex": 0 }, { "Label": "Pos2", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.5], "XYStage": [ 38561.3, 32040.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos3", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.185], "Focus": [17741.3], "XYStage": [ 37698.4, 32041.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos4", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.16], "Focus": [17741.5], "XYStage": [ 32375.4, 31758.9 ] }, "GridColumnIndex": 0 }, { "Label": "Pos5", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.17000000000002], "Focus": [17741.3], "XYStage": [ 31770.7, 31758.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos6", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.4], "XYStage": [ 26536.800000000003, 31068.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos7", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.205], "Focus": [17741.4], "XYStage": [ 25420.2, 31068.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos8", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.85], "Focus": [17741.3], "XYStage": [ 21059, 31508 ] }, "GridColumnIndex": 0 }, { "Label": "Pos9", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.82], "Focus": [17741.3], "XYStage": [ 19731.9, 31506.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos10", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.79], "Focus": [17741.4], "XYStage": [ 14528.5, 32007.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos11", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.83], "Focus": [17741.2], "XYStage": [ 13830.300000000001, 32009.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos12", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.79], "Focus": [17741.3], "XYStage": [ 8775.300000000001, 32017.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos13", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.815], "Focus": [17741.3], "XYStage": [ 8109.200000000001, 32008.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos14", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.805], "Focus": [17741.3], "XYStage": [ 3483.3, 31908.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos15", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.82], "Focus": [17741.2], "XYStage": [ 2686.8, 31903.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos16", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [211.48000000000002], "Focus": [17741.1], "XYStage": [ -1978.9, 31973.600000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos17", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [211.51500000000001], "Focus": [17741.1], "XYStage": [ -2957.8, 31982.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos18", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.195], "Focus": [17741.5], "XYStage": [ 47747.4, -576.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos19", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.23000000000002], "Focus": [17741.6], "XYStage": [ 47053.9, -576.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos20", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.205], "Focus": [17741.4], "XYStage": [ 41871.200000000004, -479.8 ] }, "GridColumnIndex": 0 }, { "Label": "Pos21", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.4], "XYStage": [ 40882.600000000006, -392 ] }, "GridColumnIndex": 0 }, { "Label": "Pos22", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.24], "Focus": [17741.4], "XYStage": [ 35905.6, -135.3 ] }, "GridColumnIndex": 0 }, { "Label": "Pos23", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.195], "Focus": [17741.5], "XYStage": [ 34990.9, -132.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos24", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.46], "Focus": [17741.4], "XYStage": [ 29678.100000000002, -958.1 ] }, "GridColumnIndex": 0 }, { "Label": "Pos25", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.44], "Focus": [17741.5], "XYStage": [ 28983.600000000002, -939.4000000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos26", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.475], "Focus": [17741.5], "XYStage": [ 23019.7, -122 ] }, "GridColumnIndex": 0 }, { "Label": "Pos27", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.44], "Focus": [17741.7], "XYStage": [ 22428, -116.80000000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos28", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.46], "Focus": [17741.7], "XYStage": [ 16364.900000000001, -181.10000000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos29", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.48000000000002], "Focus": [17741.5], "XYStage": [ 15712.1, -180 ] }, "GridColumnIndex": 0 }, { "Label": "Pos30", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.45000000000002], "Focus": [17741.7], "XYStage": [ 10346.1, 77.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos31", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.49], "Focus": [17741.4], "XYStage": [ 9755.5, 85.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos32", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.475], "Focus": [17741.5], "XYStage": [ 4775.5, 293.90000000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos33", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.445], "Focus": [17741.4], "XYStage": [ 3954.9, 293.90000000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos34", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.455], "Focus": [17741.4], "XYStage": [ -1324.9, 545.6 ] }, "GridColumnIndex": 0 }, { "Label": "Pos35", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.445], "Focus": [17741.3], "XYStage": [ -2097, 576.6 ] }, "GridColumnIndex": 0 } ], "z-step_um": 10, "GridRow": 0, "BitDepth": 16, "Depth": 2, "Channels": 6, "Source": "Micro-Manager", "ChColors": [ -16711681, -256, -65281, -1, -1, -16776961 ], "MetadataVersion": 10, "KeepShutterOpenChannels": true, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536, 65536 ], "CustomIntervals_ms": [], "Height": 1200, "MicroManagerVersion": "1.4.23 20180219", "GridColumn": 0, "UUID": "14a13bf7-c944-4672-b873-fcfbbb7ea13d", "PixelType": "GRAY16", "Width": 1200, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\Yan\\20191218-YC113-S7S8-miseq", "Frames": 1, "Comment": "", "UserName": "Nikon", "PixelSize_um": 1.1, "ROI": [ 0, 0, 1200, 1200 ], "Date": "2019-12-18", "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC", "P-DAPI-high" ], "KeepShutterOpenSlices": true, "IJType": 1, "ComputerName": "ZadorConfocal2", "Positions": 36, "PositionIndex": 9, "CameraTimeout": 20000, "TimeFirst": false }, "640 AO-Name": "AnalogIO", "Vincent-D3-Command": "Close" }, "FrameKey-0-4-5": { "COM4-StopBits": "1", "528 AO-MaxVolts": "5.0000", "XLIGHT Excitation Wheel-State": "1", "470 Shutter-Name": "AnalogIO", "Core-Shutter": "Vincent-D3", "555 AO-Demo": "No", "XLIGHT Dichroic-State": "2", "XYStage-StepSize-Y": "0.1000", "ZPositionUm": 248.82, "XYStage-StepSize-X": "0.1000", "PRIME-FirmwareVersion": "20.28", "PRIME-ClearCycles": "2", "520-Name": "DA Shutter", "405 Shutter-Name": "AnalogIO", "PIEZO-StepSize": "0.0050", "PRIME-ClearMode": "Pre-Exposure", "470 AO-MinVolts": "0.0000", "405 AO-SupportsTriggering": "1", "CameraChannelIndex": 0, "XYStage-StartSpeed": "500.0000", "COM9-StopBits": "2", "405 AO-Description": "NI DAC", "COM4-DelayBetweenCharsMs": "0.0000", "445 AO-Block voltage": "false", "520 AO-TriggerInputLine": "/Dev1/PFI0", "555 AO-Description": "NI DAC", "520 AO-TriggerSampleRate": "100000", "COM4-Verbose": "1", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM4-Name": "COM4", "405 Shutter-Volts": "0.0000", "Vincent-D3-Port": "COM1", "PRIME-PP 4 MIN ADU AFFECTED": "200", "470 Shutter-MinVolts": "0.0000", "PRIME-PP 3 ENABLED": "Yes", "470 AO-Description": "NI DAC", "PRIME-Description": "PVCAM API device adapter", "520 Shutter-MaxVolts": "5.0000", "520 Shutter-Volts": "0.0000", "528 AO-Sequenceable": "1", "PRIME-Binning": "1x1", "555 Shutter-MaxVolts": "5.0000", "528 Shutter-TriggerSequenceLength": "1000", "405 Shutter-Block voltage": "false", "XLIGHT Spin motor-State": "1", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "Vincent-D3-Time to open (ms)": "35.0000", "Vincent-D3-Time to close (ms)": "35.0000", "445 AO-TriggerSampleRate": "100000", "640 Shutter-IOChannel": "Dev1/ao14", "555 AO-Sequenceable": "1", "520 AO-Name": "AnalogIO", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "470 AO-Block voltage": "false", "COM1-Verbose": "1", "COM9-AnswerTimeout": "10000.0000", "528 Shutter-Name": "AnalogIO", "PRIME-Trigger Timeout (secs)": "10", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "NextFrame": 0, "470 AO-TriggerSequenceLength": "1000", "PRIME-TransposeXY": "0", "445 AO-TriggerSequenceLength": "1000", "COM1-Name": "COM1", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "COM9-DataBits": "8", "COM1-AnswerTimeout": "500.0000", "Camera": "", "TE2000-Version": "V2.50-V2.50-V2.10", "528 Shutter-Volts": "0.0000", "XLIGHT Hub-Name": "XLIGHT Hub", "PIEZO-Description": "Ludl stage driver adapter", "PositionName": "Pos9", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSampleRate": "100000", "PRIME-FanSpeedSetpoint": "High", "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "405 AO-TriggerSampleRate": "100000", "470 Shutter-Block voltage": "false", "470-State": "0", "COM3-Parity": "None", "405 AO-Volts": "0.0000", "PRIME-TransposeMirrorY": "0", "PRIME-ChipName": "GS144BSI", "470 AO-Volts": "0.0000", "PRIME-TransposeMirrorX": "0", "445 Shutter-SupportsTriggering": "0", "470 Shutter-SupportsTriggering": "0", "XLIGHT Hub-Description": "XLIGHT hub", "PRIME-PreampOffLimit": "10000", "470 AO-Sequenceable": "1", "528 Shutter-MaxVolts": "5.0000", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "Core-XYStage": "XYStage", "470 AO-TriggerSampleRate": "100000", "PRIME-Timing-PreTriggerDelayNs": "24672000.0000", "528-DA Device": "528 Shutter", "Focus-Description": "Nikon TE2000 focus adapter", "FilterBlock-State": "5", "520 AO-Demo": "No", "COM3-DelayBetweenCharsMs": "0.0000", "470 Shutter-Volts": "0.0000", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "PRIME-PP 2 THRESHOLD": "125", "PRIME-PP 4 THRESHOLD": "75", "XYStage-Acceleration": "4.0000", "470 Shutter-Demo": "No", "PRIME-Name": "PMPCIECam00", "640-DA Device": "640 Shutter", "405 AO-Block voltage": "false", "ElapsedTime-ms": 404173, "PRIME-Offset": "100", "445 AO-IOChannel": "Dev1/ao1", "640 AO-MaxVolts": "5.0000", "PositionIndex": 9, "PRIME-PP 1 ENABLED": "Yes", "COM1-BaudRate": "9600", "640-State": "0", "COM3-Verbose": "1", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "LudlController-TransmissionDelay": "0", "PRIME-FullWellCapacity": "62000", "PRIME-SMARTStreamingEnabled": "No", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "640 Shutter-Description": "NI DAC", "528-Description": "DA device that is used as a shutter", "COM4-Description": "Serial port driver (boost:asio)", "405 Shutter-Description": "NI DAC", "TE2000-Name": "TE2000", "528 Shutter-Description": "NI DAC", "COM9-Description": "Serial port driver (boost:asio)", "LudlController-Name": "LudlController", "445 Shutter-TriggerSequenceLength": "1000", "PRIME-SerialNumber": "A18B203008", "445 AO-Volts": "0.0000", "PRIME-X-dimension": "1200", "PRIME-ShutterCloseDelay": "0", "XLIGHT Disk slider-State": "2", "Position": "Default", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 AO-IOChannel": "Dev1/ao5", "520 AO-IOChannel": "Dev1/ao4", "528 AO-TriggerSequenceLength": "1000", "405 Shutter-MaxVolts": "5.0000", "470-DA Device": "470 Shutter", "PRIME-FTCapable": "No", "Core-ChannelGroup": "Channels", "555 AO-SupportsTriggering": "0", "PRIME-Y-dimension": "1200", "Frame": 0, "555 Shutter-Sequenceable": "1", "PRIME-Timing-ExposureTimeNs": "499998000.0000", "Core-ImageProcessor": "", "528-State": "0", "520 AO-Block voltage": "false", "XLIGHT Excitation Wheel-Label": "Excitation-2", "640 Shutter-Demo": "No", "COM3-StopBits": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "520 Shutter-IOChannel": "Dev1/ao11", "Width": 1200, "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-SupportsTriggering": "0", "640 AO-TriggerSampleRate": "100000", "520-Description": "DA device that is used as a shutter", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "405 Shutter-Sequenceable": "1", "405 Shutter-SupportsTriggering": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-TriggerSampleRate": "100000", "640 Shutter-MaxVolts": "5.0000", "555 AO-MaxVolts": "5.0000", "520 AO-TriggerSequenceLength": "1000", "COM3-BaudRate": "9600", "COM9-BaudRate": "9600", "Vincent-D3-Channel #": "Ch. #1", "PRIME-PMode": "Normal", "OpticalPath-Label": "Left(100)", "BitDepth": 16, "555 AO-TriggerSequenceLength": "1000", "445 Shutter-Description": "NI DAC", "405 AO-Sequenceable": "1", "PRIME-AcquisitionMethod": "Callbacks", "555 AO-Name": "AnalogIO", "PRIME-ShutterMode": "Pre-Exposure", "COM3-Handshaking": "Off", "445 AO-SupportsTriggering": "0", "ROI": "0-0-1200-1200", "COM9-Verbose": "1", "PRIME-Color": "OFF", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "PRIME-ShutterOpenDelay": "0", "COM1-Parity": "None", "Slice": 5, "SlicePosition": 0, "COM9-DelayBetweenCharsMs": "0.0000", "555-Name": "DA Shutter", "PIEZO-LudlSingleAxisName": "B", "XLIGHT Emission Wheel-Label": "Empty", "640 Shutter-Name": "AnalogIO", "640 AO-Sequenceable": "1", "520 Shutter-Demo": "No", "528 Shutter-Demo": "No", "470-Name": "DA Shutter", "520-DA Device": "520 Shutter", "XYStage-Description": "Ludl XY stage driver adapter", "445 Shutter-MinVolts": "0.0000", "528 AO-TriggerSampleRate": "100000", "405 AO-MinVolts": "0.0000", "405 Shutter-TriggerSequenceLength": "1000", "Height": 1200, "555 AO-Block voltage": "false", "640 Shutter-Volts": "0.0000", "PixelType": "GRAY16", "445 Shutter-Block voltage": "false", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "520 Shutter-TriggerSequenceLength": "1000", "COM9-Name": "COM9", "Core-SLM": "", "555 AO-Volts": "0.0000", "555 Shutter-Block voltage": "false", "XYStage-Speed": "1800.0000", "520 AO-MaxVolts": "5.0000", "528 Shutter-SupportsTriggering": "0", "COM1-Description": "Serial port driver (boost:asio)", "PRIME-MetadataEnabled": "No", "520 Shutter-MinVolts": "0.0000", "470 Shutter-Sequenceable": "1", "PRIME-PreampDelay": "0", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-MinVolts": "0.0000", "ChannelIndex": 4, "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Core-AutoShutter": "1", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "Exposure-ms": 20, "PRIME-PP 2 ENABLED": "Yes", "640 Shutter-TriggerSampleRate": "100000", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "470 Shutter-IOChannel": "Dev1/ao10", "405 AO-Demo": "No", "PixelSizeUm": 1.1, "470 AO-Demo": "No", "470 Shutter-MaxVolts": "5.0000", "Vincent-D3-Name": "Vincent-D3", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FileName": "img_000000000_P-DIC_005.tif", "LudlController-Reset": "Operate", "PRIME-Exposure": "20.00", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "445-Name": "DA Shutter", "555 Shutter-Name": "AnalogIO", "520 Shutter-TriggerSampleRate": "100000", "445 Shutter-IOChannel": "Dev1/ao9", "PRIME-TransposeCorrection": "0", "XYStage-StepSize": "0.1000", "445-Description": "DA device that is used as a shutter", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 AO-IOChannel": "Dev1/ao7", "520 Shutter-Name": "AnalogIO", "COM3-DataBits": "8", "640 AO-Volts": "0.0000", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "555 Shutter-IOChannel": "Dev1/ao13", "WaitInterval": null, "520 Shutter-Sequenceable": "1", "YPositionUm": 31506.2, "445-State": "0", "445-DA Device": "445 Shutter", "PRIME-CircularBufferAutoSize": "ON", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "555 AO-TriggerSampleRate": "100000", "445 Shutter-Volts": "0.0000", "XLIGHT Hub-Port": "COM3", "Core-AutoFocus": "", "COM4-Handshaking": "Off", "528 Shutter-MinVolts": "0.0000", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "445 Shutter-Name": "AnalogIO", "528 Shutter-Sequenceable": "1", "XYStage-TransposeMirrorX": "0", "XYStage-TransposeMirrorY": "0", "PRIME-ReadoutRate": "100MHz 16bit", "XYStage-Name": "Stage", "640 AO-SupportsTriggering": "0", "640-Name": "DA Shutter", "640 Shutter-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "640 AO-Block voltage": "false", "445 Shutter-Sequenceable": "1", "470 AO-MaxVolts": "5.0000", "COM4-BaudRate": "9600", "470-Description": "DA device that is used as a shutter", "520 Shutter-Description": "NI DAC", "PRIME-CCDTemperature": "-14.8500", "Time": "2019-12-18 21:37:12 -0500", "640 AO-TriggerSequenceLength": "1000", "470 AO-Name": "AnalogIO", "XLIGHT Disk slider-Label": "Disk Pos 40um", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "Binning": "1x1", "TE2000-ModelType": "1", "COM3-AnswerTimeout": "500.0000", "555 Shutter-SupportsTriggering": "0", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "TE2000-Port": "COM4", "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Description": "NI DAC", "COM4-AnswerTimeout": "500.0000", "COM1-DelayBetweenCharsMs": "0.0000", "FilterBlock-Label": "Confocal", "445 AO-Name": "AnalogIO", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-Timing-ReadoutTimeNs": "24672000.0000", "528 AO-Demo": "No", "520 AO-Sequenceable": "1", "555-Description": "DA device that is used as a shutter", "405-DA Device": "405 Shutter", "405 AO-TriggerSequenceLength": "1000", "640 AO-MinVolts": "0.0000", "640 Shutter-Block voltage": "false", "FrameIndex": 0, "PRIME-Port": "CMOS", "555 AO-MinVolts": "0.0000", "COM3-Description": "Serial port driver (boost:asio)", "528 AO-Description": "NI DAC", "555 AO-IOChannel": "Dev1/ao6", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "555 AO-TriggerInputLine": "/Dev1/PFI0", "PRIME-ActualInterval-ms": "390.5200", "LudlController-Port": "COM9", "445 Shutter-Demo": "No", "PRIME-Trigger-Expose Out-Mux": "1", "SliceIndex": 5, "PRIME-CCDTemperatureSetPoint": "-15.0000", "405 Shutter-MinVolts": "0.0000", "PRIME-ExposeOutMode": "First Row", "AxisPositions": null, "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Volts": "0.0000", "528-Name": "DA Shutter", "555-State": "0", "XLIGHT Dichroic-Label": "Di03-R635-t1", "445 AO-Sequenceable": "1", "405-Name": "DA Shutter", "405 AO-IOChannel": "Dev1/ao0", "Core-Camera": "PRIME", "Focus-Name": "Focus", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "405 Shutter-IOChannel": "Dev1/ao8", "640 AO-Demo": "No", "640 Shutter-SupportsTriggering": "0", "445 AO-Demo": "No", "555-DA Device": "555 Shutter", "470 Shutter-Description": "NI DAC", "528 AO-Volts": "0.0000", "COM1-DataBits": "8", "Vincent-D3-Address": "x", "520 AO-SupportsTriggering": "0", "405-Description": "DA device that is used as a shutter", "640 Shutter-MinVolts": "0.0000", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "405 AO-Name": "AnalogIO", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "640 AO-TriggerInputLine": "/Dev1/PFI0", "405 AO-TriggerInputLine": "/Dev1/PFI0", "640-Description": "DA device that is used as a shutter", "445 Shutter-TriggerSampleRate": "100000", "470 Shutter-TriggerSequenceLength": "1000", "COM1-StopBits": "1", "520 Shutter-SupportsTriggering": "0", "405-State": "0", "Core-Initialize": "1", "XLIGHT Emission Wheel-State": "3", "520 AO-Description": "NI DAC", "528 AO-TriggerInputLine": "/Dev1/PFI0", "445 Shutter-MaxVolts": "5.0000", "COM9-Handshaking": "Off", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "XLIGHT Spin motor-Label": "On (spinning)", "PRIME-PP 5 ENABLED": "No", "UUID": "70090c09-ad51-4ea1-a06a-d6e0103606b4", "PRIME-TriggerMode": "Internal Trigger", "COM1-Handshaking": "Off", "PRIME-Timing-PostTriggerDelayNs": "49344000.0000", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "Focus-Position": "17741.5000", "528 Shutter-Block voltage": "false", "Channel": "P-DIC", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "555 Shutter-Demo": "No", "405 Shutter-Demo": "No", "528 AO-Name": "AnalogIO", "520-State": "0", "Core-Galvo": "", "405 Shutter-TriggerSampleRate": "100000", "Focus-FocusStepSize_(nm)": "100", "PRIME-PixelType": "16bit", "COM4-DataBits": "8", "470 AO-IOChannel": "Dev1/ao2", "520 AO-MinVolts": "0.0000", "COM9-Parity": "None", "PRIME-CircularBufferEnabled": "ON", "PRIME-PP 6 Reset": "No", "640 AO-Description": "NI DAC", "Source": "PRIME", "445 AO-MinVolts": "0.0000", "PIEZO-Name": "Stage", "445 AO-Description": "NI DAC", "PRIME-CircularBufferFrameCount": "46", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "520 AO-Volts": "0.0000", "528 Shutter-TriggerSampleRate": "100000", "XPositionUm": 19731.9, "OpticalPath-State": "4", "PRIME-PP 3 THRESHOLD": "80", "PRIME-PVCAM Version": "3.7.1", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Gain": "1-HDR", "640 Shutter-TriggerSequenceLength": "1000", "528 AO-SupportsTriggering": "0", "445 AO-TriggerInputLine": "/Dev1/PFI0", "OpticalPath-Name": "OpticalPath", "PRIME-PP 4 ENABLED": "Yes", "PRIME-PVCAM Adapter Version": "1.3.29", "COM3-Name": "COM3", "405 AO-MaxVolts": "5.0000", "520 Shutter-Block voltage": "false", "PRIME-Timing-ClearingTimeNs": "49344000.0000", "Core-Focus": "PIEZO", "528 AO-Block voltage": "false", "TE2000-Description": "Nikon TE2000 hub", "528 AO-MinVolts": "0.0000", "COM4-Parity": "None", "Summary": { "Slices": 11, "ChContrastMin": [ 0, 0, 0, 0, 0, 0 ], "PixelAspect": 1, "SlicesFirst": true, "Interval_ms": 0, "Time": "2019-12-18 21:36:42 -0500", "Prefix": "seq_4", "InitialPositionList": [ { "Label": "Pos0", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.21], "Focus": [17741.5], "XYStage": [ 44542.200000000004, 31636.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos1", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.22], "Focus": [17741.4], "XYStage": [ 43801.100000000006, 31637.9 ] }, "GridColumnIndex": 0 }, { "Label": "Pos2", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.5], "XYStage": [ 38561.3, 32040.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos3", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.185], "Focus": [17741.3], "XYStage": [ 37698.4, 32041.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos4", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.16], "Focus": [17741.5], "XYStage": [ 32375.4, 31758.9 ] }, "GridColumnIndex": 0 }, { "Label": "Pos5", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.17000000000002], "Focus": [17741.3], "XYStage": [ 31770.7, 31758.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos6", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.4], "XYStage": [ 26536.800000000003, 31068.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos7", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.205], "Focus": [17741.4], "XYStage": [ 25420.2, 31068.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos8", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.85], "Focus": [17741.3], "XYStage": [ 21059, 31508 ] }, "GridColumnIndex": 0 }, { "Label": "Pos9", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.82], "Focus": [17741.3], "XYStage": [ 19731.9, 31506.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos10", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.79], "Focus": [17741.4], "XYStage": [ 14528.5, 32007.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos11", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.83], "Focus": [17741.2], "XYStage": [ 13830.300000000001, 32009.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos12", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.79], "Focus": [17741.3], "XYStage": [ 8775.300000000001, 32017.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos13", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.815], "Focus": [17741.3], "XYStage": [ 8109.200000000001, 32008.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos14", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.805], "Focus": [17741.3], "XYStage": [ 3483.3, 31908.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos15", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.82], "Focus": [17741.2], "XYStage": [ 2686.8, 31903.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos16", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [211.48000000000002], "Focus": [17741.1], "XYStage": [ -1978.9, 31973.600000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos17", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [211.51500000000001], "Focus": [17741.1], "XYStage": [ -2957.8, 31982.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos18", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.195], "Focus": [17741.5], "XYStage": [ 47747.4, -576.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos19", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.23000000000002], "Focus": [17741.6], "XYStage": [ 47053.9, -576.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos20", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.205], "Focus": [17741.4], "XYStage": [ 41871.200000000004, -479.8 ] }, "GridColumnIndex": 0 }, { "Label": "Pos21", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.4], "XYStage": [ 40882.600000000006, -392 ] }, "GridColumnIndex": 0 }, { "Label": "Pos22", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.24], "Focus": [17741.4], "XYStage": [ 35905.6, -135.3 ] }, "GridColumnIndex": 0 }, { "Label": "Pos23", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.195], "Focus": [17741.5], "XYStage": [ 34990.9, -132.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos24", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.46], "Focus": [17741.4], "XYStage": [ 29678.100000000002, -958.1 ] }, "GridColumnIndex": 0 }, { "Label": "Pos25", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.44], "Focus": [17741.5], "XYStage": [ 28983.600000000002, -939.4000000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos26", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.475], "Focus": [17741.5], "XYStage": [ 23019.7, -122 ] }, "GridColumnIndex": 0 }, { "Label": "Pos27", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.44], "Focus": [17741.7], "XYStage": [ 22428, -116.80000000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos28", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.46], "Focus": [17741.7], "XYStage": [ 16364.900000000001, -181.10000000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos29", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.48000000000002], "Focus": [17741.5], "XYStage": [ 15712.1, -180 ] }, "GridColumnIndex": 0 }, { "Label": "Pos30", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.45000000000002], "Focus": [17741.7], "XYStage": [ 10346.1, 77.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos31", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.49], "Focus": [17741.4], "XYStage": [ 9755.5, 85.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos32", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.475], "Focus": [17741.5], "XYStage": [ 4775.5, 293.90000000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos33", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.445], "Focus": [17741.4], "XYStage": [ 3954.9, 293.90000000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos34", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.455], "Focus": [17741.4], "XYStage": [ -1324.9, 545.6 ] }, "GridColumnIndex": 0 }, { "Label": "Pos35", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.445], "Focus": [17741.3], "XYStage": [ -2097, 576.6 ] }, "GridColumnIndex": 0 } ], "z-step_um": 10, "GridRow": 0, "BitDepth": 16, "Depth": 2, "Channels": 6, "Source": "Micro-Manager", "ChColors": [ -16711681, -256, -65281, -1, -1, -16776961 ], "MetadataVersion": 10, "KeepShutterOpenChannels": true, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536, 65536 ], "CustomIntervals_ms": [], "Height": 1200, "MicroManagerVersion": "1.4.23 20180219", "GridColumn": 0, "UUID": "14a13bf7-c944-4672-b873-fcfbbb7ea13d", "PixelType": "GRAY16", "Width": 1200, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\Yan\\20191218-YC113-S7S8-miseq", "Frames": 1, "Comment": "", "UserName": "Nikon", "PixelSize_um": 1.1, "ROI": [ 0, 0, 1200, 1200 ], "Date": "2019-12-18", "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC", "P-DAPI-high" ], "KeepShutterOpenSlices": true, "IJType": 1, "ComputerName": "ZadorConfocal2", "Positions": 36, "PositionIndex": 9, "CameraTimeout": 20000, "TimeFirst": false }, "640 AO-Name": "AnalogIO", "Vincent-D3-Command": "Close" }, "FrameKey-0-4-6": { "COM4-StopBits": "1", "528 AO-MaxVolts": "5.0000", "XLIGHT Excitation Wheel-State": "1", "470 Shutter-Name": "AnalogIO", "Core-Shutter": "Vincent-D3", "555 AO-Demo": "No", "XLIGHT Dichroic-State": "2", "XYStage-StepSize-Y": "0.1000", "ZPositionUm": 258.82, "XYStage-StepSize-X": "0.1000", "PRIME-FirmwareVersion": "20.28", "PRIME-ClearCycles": "2", "520-Name": "DA Shutter", "405 Shutter-Name": "AnalogIO", "PIEZO-StepSize": "0.0050", "PRIME-ClearMode": "Pre-Exposure", "470 AO-MinVolts": "0.0000", "405 AO-SupportsTriggering": "1", "CameraChannelIndex": 0, "XYStage-StartSpeed": "500.0000", "COM9-StopBits": "2", "405 AO-Description": "NI DAC", "COM4-DelayBetweenCharsMs": "0.0000", "445 AO-Block voltage": "false", "520 AO-TriggerInputLine": "/Dev1/PFI0", "555 AO-Description": "NI DAC", "520 AO-TriggerSampleRate": "100000", "COM4-Verbose": "1", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM4-Name": "COM4", "405 Shutter-Volts": "0.0000", "Vincent-D3-Port": "COM1", "PRIME-PP 4 MIN ADU AFFECTED": "200", "470 Shutter-MinVolts": "0.0000", "PRIME-PP 3 ENABLED": "Yes", "470 AO-Description": "NI DAC", "PRIME-Description": "PVCAM API device adapter", "520 Shutter-MaxVolts": "5.0000", "520 Shutter-Volts": "0.0000", "528 AO-Sequenceable": "1", "PRIME-Binning": "1x1", "555 Shutter-MaxVolts": "5.0000", "528 Shutter-TriggerSequenceLength": "1000", "405 Shutter-Block voltage": "false", "XLIGHT Spin motor-State": "1", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "Vincent-D3-Time to open (ms)": "35.0000", "Vincent-D3-Time to close (ms)": "35.0000", "445 AO-TriggerSampleRate": "100000", "640 Shutter-IOChannel": "Dev1/ao14", "555 AO-Sequenceable": "1", "520 AO-Name": "AnalogIO", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "470 AO-Block voltage": "false", "COM1-Verbose": "1", "COM9-AnswerTimeout": "10000.0000", "528 Shutter-Name": "AnalogIO", "PRIME-Trigger Timeout (secs)": "10", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "NextFrame": 0, "470 AO-TriggerSequenceLength": "1000", "PRIME-TransposeXY": "0", "445 AO-TriggerSequenceLength": "1000", "COM1-Name": "COM1", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "COM9-DataBits": "8", "COM1-AnswerTimeout": "500.0000", "Camera": "", "TE2000-Version": "V2.50-V2.50-V2.10", "528 Shutter-Volts": "0.0000", "XLIGHT Hub-Name": "XLIGHT Hub", "PIEZO-Description": "Ludl stage driver adapter", "PositionName": "Pos9", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSampleRate": "100000", "PRIME-FanSpeedSetpoint": "High", "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "405 AO-TriggerSampleRate": "100000", "470 Shutter-Block voltage": "false", "470-State": "0", "COM3-Parity": "None", "405 AO-Volts": "0.0000", "PRIME-TransposeMirrorY": "0", "PRIME-ChipName": "GS144BSI", "470 AO-Volts": "0.0000", "PRIME-TransposeMirrorX": "0", "445 Shutter-SupportsTriggering": "0", "470 Shutter-SupportsTriggering": "0", "XLIGHT Hub-Description": "XLIGHT hub", "PRIME-PreampOffLimit": "10000", "470 AO-Sequenceable": "1", "528 Shutter-MaxVolts": "5.0000", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "Core-XYStage": "XYStage", "470 AO-TriggerSampleRate": "100000", "PRIME-Timing-PreTriggerDelayNs": "24672000.0000", "528-DA Device": "528 Shutter", "Focus-Description": "Nikon TE2000 focus adapter", "FilterBlock-State": "5", "520 AO-Demo": "No", "COM3-DelayBetweenCharsMs": "0.0000", "470 Shutter-Volts": "0.0000", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "PRIME-PP 2 THRESHOLD": "125", "PRIME-PP 4 THRESHOLD": "75", "XYStage-Acceleration": "4.0000", "470 Shutter-Demo": "No", "PRIME-Name": "PMPCIECam00", "640-DA Device": "640 Shutter", "405 AO-Block voltage": "false", "ElapsedTime-ms": 404439, "PRIME-Offset": "100", "445 AO-IOChannel": "Dev1/ao1", "640 AO-MaxVolts": "5.0000", "PositionIndex": 9, "PRIME-PP 1 ENABLED": "Yes", "COM1-BaudRate": "9600", "640-State": "0", "COM3-Verbose": "1", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "LudlController-TransmissionDelay": "0", "PRIME-FullWellCapacity": "62000", "PRIME-SMARTStreamingEnabled": "No", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "640 Shutter-Description": "NI DAC", "528-Description": "DA device that is used as a shutter", "COM4-Description": "Serial port driver (boost:asio)", "405 Shutter-Description": "NI DAC", "TE2000-Name": "TE2000", "528 Shutter-Description": "NI DAC", "COM9-Description": "Serial port driver (boost:asio)", "LudlController-Name": "LudlController", "445 Shutter-TriggerSequenceLength": "1000", "PRIME-SerialNumber": "A18B203008", "445 AO-Volts": "0.0000", "PRIME-X-dimension": "1200", "PRIME-ShutterCloseDelay": "0", "XLIGHT Disk slider-State": "2", "Position": "Default", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 AO-IOChannel": "Dev1/ao5", "520 AO-IOChannel": "Dev1/ao4", "528 AO-TriggerSequenceLength": "1000", "405 Shutter-MaxVolts": "5.0000", "470-DA Device": "470 Shutter", "PRIME-FTCapable": "No", "Core-ChannelGroup": "Channels", "555 AO-SupportsTriggering": "0", "PRIME-Y-dimension": "1200", "Frame": 0, "555 Shutter-Sequenceable": "1", "PRIME-Timing-ExposureTimeNs": "499998000.0000", "Core-ImageProcessor": "", "528-State": "0", "520 AO-Block voltage": "false", "XLIGHT Excitation Wheel-Label": "Excitation-2", "640 Shutter-Demo": "No", "COM3-StopBits": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "520 Shutter-IOChannel": "Dev1/ao11", "Width": 1200, "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-SupportsTriggering": "0", "640 AO-TriggerSampleRate": "100000", "520-Description": "DA device that is used as a shutter", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "405 Shutter-Sequenceable": "1", "405 Shutter-SupportsTriggering": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-TriggerSampleRate": "100000", "640 Shutter-MaxVolts": "5.0000", "555 AO-MaxVolts": "5.0000", "520 AO-TriggerSequenceLength": "1000", "COM3-BaudRate": "9600", "COM9-BaudRate": "9600", "Vincent-D3-Channel #": "Ch. #1", "PRIME-PMode": "Normal", "OpticalPath-Label": "Left(100)", "BitDepth": 16, "555 AO-TriggerSequenceLength": "1000", "445 Shutter-Description": "NI DAC", "405 AO-Sequenceable": "1", "PRIME-AcquisitionMethod": "Callbacks", "555 AO-Name": "AnalogIO", "PRIME-ShutterMode": "Pre-Exposure", "COM3-Handshaking": "Off", "445 AO-SupportsTriggering": "0", "ROI": "0-0-1200-1200", "COM9-Verbose": "1", "PRIME-Color": "OFF", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "PRIME-ShutterOpenDelay": "0", "COM1-Parity": "None", "Slice": 6, "SlicePosition": 10, "COM9-DelayBetweenCharsMs": "0.0000", "555-Name": "DA Shutter", "PIEZO-LudlSingleAxisName": "B", "XLIGHT Emission Wheel-Label": "Empty", "640 Shutter-Name": "AnalogIO", "640 AO-Sequenceable": "1", "520 Shutter-Demo": "No", "528 Shutter-Demo": "No", "470-Name": "DA Shutter", "520-DA Device": "520 Shutter", "XYStage-Description": "Ludl XY stage driver adapter", "445 Shutter-MinVolts": "0.0000", "528 AO-TriggerSampleRate": "100000", "405 AO-MinVolts": "0.0000", "405 Shutter-TriggerSequenceLength": "1000", "Height": 1200, "555 AO-Block voltage": "false", "640 Shutter-Volts": "0.0000", "PixelType": "GRAY16", "445 Shutter-Block voltage": "false", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "520 Shutter-TriggerSequenceLength": "1000", "COM9-Name": "COM9", "Core-SLM": "", "555 AO-Volts": "0.0000", "555 Shutter-Block voltage": "false", "XYStage-Speed": "1800.0000", "520 AO-MaxVolts": "5.0000", "528 Shutter-SupportsTriggering": "0", "COM1-Description": "Serial port driver (boost:asio)", "PRIME-MetadataEnabled": "No", "520 Shutter-MinVolts": "0.0000", "470 Shutter-Sequenceable": "1", "PRIME-PreampDelay": "0", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-MinVolts": "0.0000", "ChannelIndex": 4, "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Core-AutoShutter": "1", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "Exposure-ms": 20, "PRIME-PP 2 ENABLED": "Yes", "640 Shutter-TriggerSampleRate": "100000", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "470 Shutter-IOChannel": "Dev1/ao10", "405 AO-Demo": "No", "PixelSizeUm": 1.1, "470 AO-Demo": "No", "470 Shutter-MaxVolts": "5.0000", "Vincent-D3-Name": "Vincent-D3", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FileName": "img_000000000_P-DIC_006.tif", "LudlController-Reset": "Operate", "PRIME-Exposure": "20.00", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "445-Name": "DA Shutter", "555 Shutter-Name": "AnalogIO", "520 Shutter-TriggerSampleRate": "100000", "445 Shutter-IOChannel": "Dev1/ao9", "PRIME-TransposeCorrection": "0", "XYStage-StepSize": "0.1000", "445-Description": "DA device that is used as a shutter", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 AO-IOChannel": "Dev1/ao7", "520 Shutter-Name": "AnalogIO", "COM3-DataBits": "8", "640 AO-Volts": "0.0000", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "555 Shutter-IOChannel": "Dev1/ao13", "WaitInterval": null, "520 Shutter-Sequenceable": "1", "YPositionUm": 31506.2, "445-State": "0", "445-DA Device": "445 Shutter", "PRIME-CircularBufferAutoSize": "ON", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "555 AO-TriggerSampleRate": "100000", "445 Shutter-Volts": "0.0000", "XLIGHT Hub-Port": "COM3", "Core-AutoFocus": "", "COM4-Handshaking": "Off", "528 Shutter-MinVolts": "0.0000", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "445 Shutter-Name": "AnalogIO", "528 Shutter-Sequenceable": "1", "XYStage-TransposeMirrorX": "0", "XYStage-TransposeMirrorY": "0", "PRIME-ReadoutRate": "100MHz 16bit", "XYStage-Name": "Stage", "640 AO-SupportsTriggering": "0", "640-Name": "DA Shutter", "640 Shutter-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "640 AO-Block voltage": "false", "445 Shutter-Sequenceable": "1", "470 AO-MaxVolts": "5.0000", "COM4-BaudRate": "9600", "470-Description": "DA device that is used as a shutter", "520 Shutter-Description": "NI DAC", "PRIME-CCDTemperature": "-14.8500", "Time": "2019-12-18 21:37:12 -0500", "640 AO-TriggerSequenceLength": "1000", "470 AO-Name": "AnalogIO", "XLIGHT Disk slider-Label": "Disk Pos 40um", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "Binning": "1x1", "TE2000-ModelType": "1", "COM3-AnswerTimeout": "500.0000", "555 Shutter-SupportsTriggering": "0", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "TE2000-Port": "COM4", "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Description": "NI DAC", "COM4-AnswerTimeout": "500.0000", "COM1-DelayBetweenCharsMs": "0.0000", "FilterBlock-Label": "Confocal", "445 AO-Name": "AnalogIO", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-Timing-ReadoutTimeNs": "24672000.0000", "528 AO-Demo": "No", "520 AO-Sequenceable": "1", "555-Description": "DA device that is used as a shutter", "405-DA Device": "405 Shutter", "405 AO-TriggerSequenceLength": "1000", "640 AO-MinVolts": "0.0000", "640 Shutter-Block voltage": "false", "FrameIndex": 0, "PRIME-Port": "CMOS", "555 AO-MinVolts": "0.0000", "COM3-Description": "Serial port driver (boost:asio)", "528 AO-Description": "NI DAC", "555 AO-IOChannel": "Dev1/ao6", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "555 AO-TriggerInputLine": "/Dev1/PFI0", "PRIME-ActualInterval-ms": "390.5200", "LudlController-Port": "COM9", "445 Shutter-Demo": "No", "PRIME-Trigger-Expose Out-Mux": "1", "SliceIndex": 6, "PRIME-CCDTemperatureSetPoint": "-15.0000", "405 Shutter-MinVolts": "0.0000", "PRIME-ExposeOutMode": "First Row", "AxisPositions": null, "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Volts": "0.0000", "528-Name": "DA Shutter", "555-State": "0", "XLIGHT Dichroic-Label": "Di03-R635-t1", "445 AO-Sequenceable": "1", "405-Name": "DA Shutter", "405 AO-IOChannel": "Dev1/ao0", "Core-Camera": "PRIME", "Focus-Name": "Focus", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "405 Shutter-IOChannel": "Dev1/ao8", "640 AO-Demo": "No", "640 Shutter-SupportsTriggering": "0", "445 AO-Demo": "No", "555-DA Device": "555 Shutter", "470 Shutter-Description": "NI DAC", "528 AO-Volts": "0.0000", "COM1-DataBits": "8", "Vincent-D3-Address": "x", "520 AO-SupportsTriggering": "0", "405-Description": "DA device that is used as a shutter", "640 Shutter-MinVolts": "0.0000", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "405 AO-Name": "AnalogIO", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "640 AO-TriggerInputLine": "/Dev1/PFI0", "405 AO-TriggerInputLine": "/Dev1/PFI0", "640-Description": "DA device that is used as a shutter", "445 Shutter-TriggerSampleRate": "100000", "470 Shutter-TriggerSequenceLength": "1000", "COM1-StopBits": "1", "520 Shutter-SupportsTriggering": "0", "405-State": "0", "Core-Initialize": "1", "XLIGHT Emission Wheel-State": "3", "520 AO-Description": "NI DAC", "528 AO-TriggerInputLine": "/Dev1/PFI0", "445 Shutter-MaxVolts": "5.0000", "COM9-Handshaking": "Off", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "XLIGHT Spin motor-Label": "On (spinning)", "PRIME-PP 5 ENABLED": "No", "UUID": "3f2da74f-28ee-417e-8700-669ce8a8d80e", "PRIME-TriggerMode": "Internal Trigger", "COM1-Handshaking": "Off", "PRIME-Timing-PostTriggerDelayNs": "49344000.0000", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "Focus-Position": "17741.5000", "528 Shutter-Block voltage": "false", "Channel": "P-DIC", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "555 Shutter-Demo": "No", "405 Shutter-Demo": "No", "528 AO-Name": "AnalogIO", "520-State": "0", "Core-Galvo": "", "405 Shutter-TriggerSampleRate": "100000", "Focus-FocusStepSize_(nm)": "100", "PRIME-PixelType": "16bit", "COM4-DataBits": "8", "470 AO-IOChannel": "Dev1/ao2", "520 AO-MinVolts": "0.0000", "COM9-Parity": "None", "PRIME-CircularBufferEnabled": "ON", "PRIME-PP 6 Reset": "No", "640 AO-Description": "NI DAC", "Source": "PRIME", "445 AO-MinVolts": "0.0000", "PIEZO-Name": "Stage", "445 AO-Description": "NI DAC", "PRIME-CircularBufferFrameCount": "46", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "520 AO-Volts": "0.0000", "528 Shutter-TriggerSampleRate": "100000", "XPositionUm": 19731.9, "OpticalPath-State": "4", "PRIME-PP 3 THRESHOLD": "80", "PRIME-PVCAM Version": "3.7.1", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Gain": "1-HDR", "640 Shutter-TriggerSequenceLength": "1000", "528 AO-SupportsTriggering": "0", "445 AO-TriggerInputLine": "/Dev1/PFI0", "OpticalPath-Name": "OpticalPath", "PRIME-PP 4 ENABLED": "Yes", "PRIME-PVCAM Adapter Version": "1.3.29", "COM3-Name": "COM3", "405 AO-MaxVolts": "5.0000", "520 Shutter-Block voltage": "false", "PRIME-Timing-ClearingTimeNs": "49344000.0000", "Core-Focus": "PIEZO", "528 AO-Block voltage": "false", "TE2000-Description": "Nikon TE2000 hub", "528 AO-MinVolts": "0.0000", "COM4-Parity": "None", "Summary": { "Slices": 11, "ChContrastMin": [ 0, 0, 0, 0, 0, 0 ], "PixelAspect": 1, "SlicesFirst": true, "Interval_ms": 0, "Time": "2019-12-18 21:36:42 -0500", "Prefix": "seq_4", "InitialPositionList": [ { "Label": "Pos0", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.21], "Focus": [17741.5], "XYStage": [ 44542.200000000004, 31636.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos1", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.22], "Focus": [17741.4], "XYStage": [ 43801.100000000006, 31637.9 ] }, "GridColumnIndex": 0 }, { "Label": "Pos2", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.5], "XYStage": [ 38561.3, 32040.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos3", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.185], "Focus": [17741.3], "XYStage": [ 37698.4, 32041.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos4", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.16], "Focus": [17741.5], "XYStage": [ 32375.4, 31758.9 ] }, "GridColumnIndex": 0 }, { "Label": "Pos5", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.17000000000002], "Focus": [17741.3], "XYStage": [ 31770.7, 31758.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos6", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.4], "XYStage": [ 26536.800000000003, 31068.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos7", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.205], "Focus": [17741.4], "XYStage": [ 25420.2, 31068.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos8", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.85], "Focus": [17741.3], "XYStage": [ 21059, 31508 ] }, "GridColumnIndex": 0 }, { "Label": "Pos9", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.82], "Focus": [17741.3], "XYStage": [ 19731.9, 31506.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos10", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.79], "Focus": [17741.4], "XYStage": [ 14528.5, 32007.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos11", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.83], "Focus": [17741.2], "XYStage": [ 13830.300000000001, 32009.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos12", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.79], "Focus": [17741.3], "XYStage": [ 8775.300000000001, 32017.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos13", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.815], "Focus": [17741.3], "XYStage": [ 8109.200000000001, 32008.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos14", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.805], "Focus": [17741.3], "XYStage": [ 3483.3, 31908.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos15", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.82], "Focus": [17741.2], "XYStage": [ 2686.8, 31903.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos16", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [211.48000000000002], "Focus": [17741.1], "XYStage": [ -1978.9, 31973.600000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos17", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [211.51500000000001], "Focus": [17741.1], "XYStage": [ -2957.8, 31982.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos18", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.195], "Focus": [17741.5], "XYStage": [ 47747.4, -576.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos19", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.23000000000002], "Focus": [17741.6], "XYStage": [ 47053.9, -576.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos20", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.205], "Focus": [17741.4], "XYStage": [ 41871.200000000004, -479.8 ] }, "GridColumnIndex": 0 }, { "Label": "Pos21", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.4], "XYStage": [ 40882.600000000006, -392 ] }, "GridColumnIndex": 0 }, { "Label": "Pos22", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.24], "Focus": [17741.4], "XYStage": [ 35905.6, -135.3 ] }, "GridColumnIndex": 0 }, { "Label": "Pos23", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.195], "Focus": [17741.5], "XYStage": [ 34990.9, -132.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos24", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.46], "Focus": [17741.4], "XYStage": [ 29678.100000000002, -958.1 ] }, "GridColumnIndex": 0 }, { "Label": "Pos25", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.44], "Focus": [17741.5], "XYStage": [ 28983.600000000002, -939.4000000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos26", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.475], "Focus": [17741.5], "XYStage": [ 23019.7, -122 ] }, "GridColumnIndex": 0 }, { "Label": "Pos27", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.44], "Focus": [17741.7], "XYStage": [ 22428, -116.80000000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos28", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.46], "Focus": [17741.7], "XYStage": [ 16364.900000000001, -181.10000000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos29", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.48000000000002], "Focus": [17741.5], "XYStage": [ 15712.1, -180 ] }, "GridColumnIndex": 0 }, { "Label": "Pos30", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.45000000000002], "Focus": [17741.7], "XYStage": [ 10346.1, 77.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos31", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.49], "Focus": [17741.4], "XYStage": [ 9755.5, 85.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos32", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.475], "Focus": [17741.5], "XYStage": [ 4775.5, 293.90000000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos33", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.445], "Focus": [17741.4], "XYStage": [ 3954.9, 293.90000000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos34", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.455], "Focus": [17741.4], "XYStage": [ -1324.9, 545.6 ] }, "GridColumnIndex": 0 }, { "Label": "Pos35", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.445], "Focus": [17741.3], "XYStage": [ -2097, 576.6 ] }, "GridColumnIndex": 0 } ], "z-step_um": 10, "GridRow": 0, "BitDepth": 16, "Depth": 2, "Channels": 6, "Source": "Micro-Manager", "ChColors": [ -16711681, -256, -65281, -1, -1, -16776961 ], "MetadataVersion": 10, "KeepShutterOpenChannels": true, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536, 65536 ], "CustomIntervals_ms": [], "Height": 1200, "MicroManagerVersion": "1.4.23 20180219", "GridColumn": 0, "UUID": "14a13bf7-c944-4672-b873-fcfbbb7ea13d", "PixelType": "GRAY16", "Width": 1200, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\Yan\\20191218-YC113-S7S8-miseq", "Frames": 1, "Comment": "", "UserName": "Nikon", "PixelSize_um": 1.1, "ROI": [ 0, 0, 1200, 1200 ], "Date": "2019-12-18", "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC", "P-DAPI-high" ], "KeepShutterOpenSlices": true, "IJType": 1, "ComputerName": "ZadorConfocal2", "Positions": 36, "PositionIndex": 9, "CameraTimeout": 20000, "TimeFirst": false }, "640 AO-Name": "AnalogIO", "Vincent-D3-Command": "Close" }, "FrameKey-0-4-7": { "COM4-StopBits": "1", "528 AO-MaxVolts": "5.0000", "XLIGHT Excitation Wheel-State": "1", "470 Shutter-Name": "AnalogIO", "Core-Shutter": "Vincent-D3", "555 AO-Demo": "No", "XLIGHT Dichroic-State": "2", "XYStage-StepSize-Y": "0.1000", "ZPositionUm": 268.82, "XYStage-StepSize-X": "0.1000", "PRIME-FirmwareVersion": "20.28", "PRIME-ClearCycles": "2", "520-Name": "DA Shutter", "405 Shutter-Name": "AnalogIO", "PIEZO-StepSize": "0.0050", "PRIME-ClearMode": "Pre-Exposure", "470 AO-MinVolts": "0.0000", "405 AO-SupportsTriggering": "1", "CameraChannelIndex": 0, "XYStage-StartSpeed": "500.0000", "COM9-StopBits": "2", "405 AO-Description": "NI DAC", "COM4-DelayBetweenCharsMs": "0.0000", "445 AO-Block voltage": "false", "520 AO-TriggerInputLine": "/Dev1/PFI0", "555 AO-Description": "NI DAC", "520 AO-TriggerSampleRate": "100000", "COM4-Verbose": "1", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM4-Name": "COM4", "405 Shutter-Volts": "0.0000", "Vincent-D3-Port": "COM1", "PRIME-PP 4 MIN ADU AFFECTED": "200", "470 Shutter-MinVolts": "0.0000", "PRIME-PP 3 ENABLED": "Yes", "470 AO-Description": "NI DAC", "PRIME-Description": "PVCAM API device adapter", "520 Shutter-MaxVolts": "5.0000", "520 Shutter-Volts": "0.0000", "528 AO-Sequenceable": "1", "PRIME-Binning": "1x1", "555 Shutter-MaxVolts": "5.0000", "528 Shutter-TriggerSequenceLength": "1000", "405 Shutter-Block voltage": "false", "XLIGHT Spin motor-State": "1", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "Vincent-D3-Time to open (ms)": "35.0000", "Vincent-D3-Time to close (ms)": "35.0000", "445 AO-TriggerSampleRate": "100000", "640 Shutter-IOChannel": "Dev1/ao14", "555 AO-Sequenceable": "1", "520 AO-Name": "AnalogIO", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "470 AO-Block voltage": "false", "COM1-Verbose": "1", "COM9-AnswerTimeout": "10000.0000", "528 Shutter-Name": "AnalogIO", "PRIME-Trigger Timeout (secs)": "10", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "NextFrame": 0, "470 AO-TriggerSequenceLength": "1000", "PRIME-TransposeXY": "0", "445 AO-TriggerSequenceLength": "1000", "COM1-Name": "COM1", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "COM9-DataBits": "8", "COM1-AnswerTimeout": "500.0000", "Camera": "", "TE2000-Version": "V2.50-V2.50-V2.10", "528 Shutter-Volts": "0.0000", "XLIGHT Hub-Name": "XLIGHT Hub", "PIEZO-Description": "Ludl stage driver adapter", "PositionName": "Pos9", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSampleRate": "100000", "PRIME-FanSpeedSetpoint": "High", "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "405 AO-TriggerSampleRate": "100000", "470 Shutter-Block voltage": "false", "470-State": "0", "COM3-Parity": "None", "405 AO-Volts": "0.0000", "PRIME-TransposeMirrorY": "0", "PRIME-ChipName": "GS144BSI", "470 AO-Volts": "0.0000", "PRIME-TransposeMirrorX": "0", "445 Shutter-SupportsTriggering": "0", "470 Shutter-SupportsTriggering": "0", "XLIGHT Hub-Description": "XLIGHT hub", "PRIME-PreampOffLimit": "10000", "470 AO-Sequenceable": "1", "528 Shutter-MaxVolts": "5.0000", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "Core-XYStage": "XYStage", "470 AO-TriggerSampleRate": "100000", "PRIME-Timing-PreTriggerDelayNs": "24672000.0000", "528-DA Device": "528 Shutter", "Focus-Description": "Nikon TE2000 focus adapter", "FilterBlock-State": "5", "520 AO-Demo": "No", "COM3-DelayBetweenCharsMs": "0.0000", "470 Shutter-Volts": "0.0000", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "PRIME-PP 2 THRESHOLD": "125", "PRIME-PP 4 THRESHOLD": "75", "XYStage-Acceleration": "4.0000", "470 Shutter-Demo": "No", "PRIME-Name": "PMPCIECam00", "640-DA Device": "640 Shutter", "405 AO-Block voltage": "false", "ElapsedTime-ms": 404710, "PRIME-Offset": "100", "445 AO-IOChannel": "Dev1/ao1", "640 AO-MaxVolts": "5.0000", "PositionIndex": 9, "PRIME-PP 1 ENABLED": "Yes", "COM1-BaudRate": "9600", "640-State": "0", "COM3-Verbose": "1", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "LudlController-TransmissionDelay": "0", "PRIME-FullWellCapacity": "62000", "PRIME-SMARTStreamingEnabled": "No", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "640 Shutter-Description": "NI DAC", "528-Description": "DA device that is used as a shutter", "COM4-Description": "Serial port driver (boost:asio)", "405 Shutter-Description": "NI DAC", "TE2000-Name": "TE2000", "528 Shutter-Description": "NI DAC", "COM9-Description": "Serial port driver (boost:asio)", "LudlController-Name": "LudlController", "445 Shutter-TriggerSequenceLength": "1000", "PRIME-SerialNumber": "A18B203008", "445 AO-Volts": "0.0000", "PRIME-X-dimension": "1200", "PRIME-ShutterCloseDelay": "0", "XLIGHT Disk slider-State": "2", "Position": "Default", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 AO-IOChannel": "Dev1/ao5", "520 AO-IOChannel": "Dev1/ao4", "528 AO-TriggerSequenceLength": "1000", "405 Shutter-MaxVolts": "5.0000", "470-DA Device": "470 Shutter", "PRIME-FTCapable": "No", "Core-ChannelGroup": "Channels", "555 AO-SupportsTriggering": "0", "PRIME-Y-dimension": "1200", "Frame": 0, "555 Shutter-Sequenceable": "1", "PRIME-Timing-ExposureTimeNs": "499998000.0000", "Core-ImageProcessor": "", "528-State": "0", "520 AO-Block voltage": "false", "XLIGHT Excitation Wheel-Label": "Excitation-2", "640 Shutter-Demo": "No", "COM3-StopBits": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "520 Shutter-IOChannel": "Dev1/ao11", "Width": 1200, "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-SupportsTriggering": "0", "640 AO-TriggerSampleRate": "100000", "520-Description": "DA device that is used as a shutter", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "405 Shutter-Sequenceable": "1", "405 Shutter-SupportsTriggering": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-TriggerSampleRate": "100000", "640 Shutter-MaxVolts": "5.0000", "555 AO-MaxVolts": "5.0000", "520 AO-TriggerSequenceLength": "1000", "COM3-BaudRate": "9600", "COM9-BaudRate": "9600", "Vincent-D3-Channel #": "Ch. #1", "PRIME-PMode": "Normal", "OpticalPath-Label": "Left(100)", "BitDepth": 16, "555 AO-TriggerSequenceLength": "1000", "445 Shutter-Description": "NI DAC", "405 AO-Sequenceable": "1", "PRIME-AcquisitionMethod": "Callbacks", "555 AO-Name": "AnalogIO", "PRIME-ShutterMode": "Pre-Exposure", "COM3-Handshaking": "Off", "445 AO-SupportsTriggering": "0", "ROI": "0-0-1200-1200", "COM9-Verbose": "1", "PRIME-Color": "OFF", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "PRIME-ShutterOpenDelay": "0", "COM1-Parity": "None", "Slice": 7, "SlicePosition": 20, "COM9-DelayBetweenCharsMs": "0.0000", "555-Name": "DA Shutter", "PIEZO-LudlSingleAxisName": "B", "XLIGHT Emission Wheel-Label": "Empty", "640 Shutter-Name": "AnalogIO", "640 AO-Sequenceable": "1", "520 Shutter-Demo": "No", "528 Shutter-Demo": "No", "470-Name": "DA Shutter", "520-DA Device": "520 Shutter", "XYStage-Description": "Ludl XY stage driver adapter", "445 Shutter-MinVolts": "0.0000", "528 AO-TriggerSampleRate": "100000", "405 AO-MinVolts": "0.0000", "405 Shutter-TriggerSequenceLength": "1000", "Height": 1200, "555 AO-Block voltage": "false", "640 Shutter-Volts": "0.0000", "PixelType": "GRAY16", "445 Shutter-Block voltage": "false", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "520 Shutter-TriggerSequenceLength": "1000", "COM9-Name": "COM9", "Core-SLM": "", "555 AO-Volts": "0.0000", "555 Shutter-Block voltage": "false", "XYStage-Speed": "1800.0000", "520 AO-MaxVolts": "5.0000", "528 Shutter-SupportsTriggering": "0", "COM1-Description": "Serial port driver (boost:asio)", "PRIME-MetadataEnabled": "No", "520 Shutter-MinVolts": "0.0000", "470 Shutter-Sequenceable": "1", "PRIME-PreampDelay": "0", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-MinVolts": "0.0000", "ChannelIndex": 4, "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Core-AutoShutter": "1", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "Exposure-ms": 20, "PRIME-PP 2 ENABLED": "Yes", "640 Shutter-TriggerSampleRate": "100000", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "470 Shutter-IOChannel": "Dev1/ao10", "405 AO-Demo": "No", "PixelSizeUm": 1.1, "470 AO-Demo": "No", "470 Shutter-MaxVolts": "5.0000", "Vincent-D3-Name": "Vincent-D3", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FileName": "img_000000000_P-DIC_007.tif", "LudlController-Reset": "Operate", "PRIME-Exposure": "20.00", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "445-Name": "DA Shutter", "555 Shutter-Name": "AnalogIO", "520 Shutter-TriggerSampleRate": "100000", "445 Shutter-IOChannel": "Dev1/ao9", "PRIME-TransposeCorrection": "0", "XYStage-StepSize": "0.1000", "445-Description": "DA device that is used as a shutter", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 AO-IOChannel": "Dev1/ao7", "520 Shutter-Name": "AnalogIO", "COM3-DataBits": "8", "640 AO-Volts": "0.0000", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "555 Shutter-IOChannel": "Dev1/ao13", "WaitInterval": null, "520 Shutter-Sequenceable": "1", "YPositionUm": 31506.2, "445-State": "0", "445-DA Device": "445 Shutter", "PRIME-CircularBufferAutoSize": "ON", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "555 AO-TriggerSampleRate": "100000", "445 Shutter-Volts": "0.0000", "XLIGHT Hub-Port": "COM3", "Core-AutoFocus": "", "COM4-Handshaking": "Off", "528 Shutter-MinVolts": "0.0000", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "445 Shutter-Name": "AnalogIO", "528 Shutter-Sequenceable": "1", "XYStage-TransposeMirrorX": "0", "XYStage-TransposeMirrorY": "0", "PRIME-ReadoutRate": "100MHz 16bit", "XYStage-Name": "Stage", "640 AO-SupportsTriggering": "0", "640-Name": "DA Shutter", "640 Shutter-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "640 AO-Block voltage": "false", "445 Shutter-Sequenceable": "1", "470 AO-MaxVolts": "5.0000", "COM4-BaudRate": "9600", "470-Description": "DA device that is used as a shutter", "520 Shutter-Description": "NI DAC", "PRIME-CCDTemperature": "-14.8500", "Time": "2019-12-18 21:37:12 -0500", "640 AO-TriggerSequenceLength": "1000", "470 AO-Name": "AnalogIO", "XLIGHT Disk slider-Label": "Disk Pos 40um", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "Binning": "1x1", "TE2000-ModelType": "1", "COM3-AnswerTimeout": "500.0000", "555 Shutter-SupportsTriggering": "0", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "TE2000-Port": "COM4", "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Description": "NI DAC", "COM4-AnswerTimeout": "500.0000", "COM1-DelayBetweenCharsMs": "0.0000", "FilterBlock-Label": "Confocal", "445 AO-Name": "AnalogIO", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-Timing-ReadoutTimeNs": "24672000.0000", "528 AO-Demo": "No", "520 AO-Sequenceable": "1", "555-Description": "DA device that is used as a shutter", "405-DA Device": "405 Shutter", "405 AO-TriggerSequenceLength": "1000", "640 AO-MinVolts": "0.0000", "640 Shutter-Block voltage": "false", "FrameIndex": 0, "PRIME-Port": "CMOS", "555 AO-MinVolts": "0.0000", "COM3-Description": "Serial port driver (boost:asio)", "528 AO-Description": "NI DAC", "555 AO-IOChannel": "Dev1/ao6", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "555 AO-TriggerInputLine": "/Dev1/PFI0", "PRIME-ActualInterval-ms": "390.5200", "LudlController-Port": "COM9", "445 Shutter-Demo": "No", "PRIME-Trigger-Expose Out-Mux": "1", "SliceIndex": 7, "PRIME-CCDTemperatureSetPoint": "-15.0000", "405 Shutter-MinVolts": "0.0000", "PRIME-ExposeOutMode": "First Row", "AxisPositions": null, "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Volts": "0.0000", "528-Name": "DA Shutter", "555-State": "0", "XLIGHT Dichroic-Label": "Di03-R635-t1", "445 AO-Sequenceable": "1", "405-Name": "DA Shutter", "405 AO-IOChannel": "Dev1/ao0", "Core-Camera": "PRIME", "Focus-Name": "Focus", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "405 Shutter-IOChannel": "Dev1/ao8", "640 AO-Demo": "No", "640 Shutter-SupportsTriggering": "0", "445 AO-Demo": "No", "555-DA Device": "555 Shutter", "470 Shutter-Description": "NI DAC", "528 AO-Volts": "0.0000", "COM1-DataBits": "8", "Vincent-D3-Address": "x", "520 AO-SupportsTriggering": "0", "405-Description": "DA device that is used as a shutter", "640 Shutter-MinVolts": "0.0000", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "405 AO-Name": "AnalogIO", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "640 AO-TriggerInputLine": "/Dev1/PFI0", "405 AO-TriggerInputLine": "/Dev1/PFI0", "640-Description": "DA device that is used as a shutter", "445 Shutter-TriggerSampleRate": "100000", "470 Shutter-TriggerSequenceLength": "1000", "COM1-StopBits": "1", "520 Shutter-SupportsTriggering": "0", "405-State": "0", "Core-Initialize": "1", "XLIGHT Emission Wheel-State": "3", "520 AO-Description": "NI DAC", "528 AO-TriggerInputLine": "/Dev1/PFI0", "445 Shutter-MaxVolts": "5.0000", "COM9-Handshaking": "Off", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "XLIGHT Spin motor-Label": "On (spinning)", "PRIME-PP 5 ENABLED": "No", "UUID": "71509f86-cb67-4833-9cba-c092c0bdb76f", "PRIME-TriggerMode": "Internal Trigger", "COM1-Handshaking": "Off", "PRIME-Timing-PostTriggerDelayNs": "49344000.0000", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "Focus-Position": "17741.5000", "528 Shutter-Block voltage": "false", "Channel": "P-DIC", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "555 Shutter-Demo": "No", "405 Shutter-Demo": "No", "528 AO-Name": "AnalogIO", "520-State": "0", "Core-Galvo": "", "405 Shutter-TriggerSampleRate": "100000", "Focus-FocusStepSize_(nm)": "100", "PRIME-PixelType": "16bit", "COM4-DataBits": "8", "470 AO-IOChannel": "Dev1/ao2", "520 AO-MinVolts": "0.0000", "COM9-Parity": "None", "PRIME-CircularBufferEnabled": "ON", "PRIME-PP 6 Reset": "No", "640 AO-Description": "NI DAC", "Source": "PRIME", "445 AO-MinVolts": "0.0000", "PIEZO-Name": "Stage", "445 AO-Description": "NI DAC", "PRIME-CircularBufferFrameCount": "46", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "520 AO-Volts": "0.0000", "528 Shutter-TriggerSampleRate": "100000", "XPositionUm": 19731.9, "OpticalPath-State": "4", "PRIME-PP 3 THRESHOLD": "80", "PRIME-PVCAM Version": "3.7.1", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Gain": "1-HDR", "640 Shutter-TriggerSequenceLength": "1000", "528 AO-SupportsTriggering": "0", "445 AO-TriggerInputLine": "/Dev1/PFI0", "OpticalPath-Name": "OpticalPath", "PRIME-PP 4 ENABLED": "Yes", "PRIME-PVCAM Adapter Version": "1.3.29", "COM3-Name": "COM3", "405 AO-MaxVolts": "5.0000", "520 Shutter-Block voltage": "false", "PRIME-Timing-ClearingTimeNs": "49344000.0000", "Core-Focus": "PIEZO", "528 AO-Block voltage": "false", "TE2000-Description": "Nikon TE2000 hub", "528 AO-MinVolts": "0.0000", "COM4-Parity": "None", "Summary": { "Slices": 11, "ChContrastMin": [ 0, 0, 0, 0, 0, 0 ], "PixelAspect": 1, "SlicesFirst": true, "Interval_ms": 0, "Time": "2019-12-18 21:36:42 -0500", "Prefix": "seq_4", "InitialPositionList": [ { "Label": "Pos0", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.21], "Focus": [17741.5], "XYStage": [ 44542.200000000004, 31636.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos1", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.22], "Focus": [17741.4], "XYStage": [ 43801.100000000006, 31637.9 ] }, "GridColumnIndex": 0 }, { "Label": "Pos2", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.5], "XYStage": [ 38561.3, 32040.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos3", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.185], "Focus": [17741.3], "XYStage": [ 37698.4, 32041.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos4", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.16], "Focus": [17741.5], "XYStage": [ 32375.4, 31758.9 ] }, "GridColumnIndex": 0 }, { "Label": "Pos5", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.17000000000002], "Focus": [17741.3], "XYStage": [ 31770.7, 31758.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos6", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.4], "XYStage": [ 26536.800000000003, 31068.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos7", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.205], "Focus": [17741.4], "XYStage": [ 25420.2, 31068.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos8", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.85], "Focus": [17741.3], "XYStage": [ 21059, 31508 ] }, "GridColumnIndex": 0 }, { "Label": "Pos9", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.82], "Focus": [17741.3], "XYStage": [ 19731.9, 31506.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos10", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.79], "Focus": [17741.4], "XYStage": [ 14528.5, 32007.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos11", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.83], "Focus": [17741.2], "XYStage": [ 13830.300000000001, 32009.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos12", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.79], "Focus": [17741.3], "XYStage": [ 8775.300000000001, 32017.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos13", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.815], "Focus": [17741.3], "XYStage": [ 8109.200000000001, 32008.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos14", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.805], "Focus": [17741.3], "XYStage": [ 3483.3, 31908.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos15", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.82], "Focus": [17741.2], "XYStage": [ 2686.8, 31903.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos16", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [211.48000000000002], "Focus": [17741.1], "XYStage": [ -1978.9, 31973.600000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos17", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [211.51500000000001], "Focus": [17741.1], "XYStage": [ -2957.8, 31982.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos18", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.195], "Focus": [17741.5], "XYStage": [ 47747.4, -576.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos19", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.23000000000002], "Focus": [17741.6], "XYStage": [ 47053.9, -576.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos20", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.205], "Focus": [17741.4], "XYStage": [ 41871.200000000004, -479.8 ] }, "GridColumnIndex": 0 }, { "Label": "Pos21", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.4], "XYStage": [ 40882.600000000006, -392 ] }, "GridColumnIndex": 0 }, { "Label": "Pos22", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.24], "Focus": [17741.4], "XYStage": [ 35905.6, -135.3 ] }, "GridColumnIndex": 0 }, { "Label": "Pos23", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.195], "Focus": [17741.5], "XYStage": [ 34990.9, -132.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos24", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.46], "Focus": [17741.4], "XYStage": [ 29678.100000000002, -958.1 ] }, "GridColumnIndex": 0 }, { "Label": "Pos25", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.44], "Focus": [17741.5], "XYStage": [ 28983.600000000002, -939.4000000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos26", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.475], "Focus": [17741.5], "XYStage": [ 23019.7, -122 ] }, "GridColumnIndex": 0 }, { "Label": "Pos27", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.44], "Focus": [17741.7], "XYStage": [ 22428, -116.80000000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos28", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.46], "Focus": [17741.7], "XYStage": [ 16364.900000000001, -181.10000000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos29", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.48000000000002], "Focus": [17741.5], "XYStage": [ 15712.1, -180 ] }, "GridColumnIndex": 0 }, { "Label": "Pos30", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.45000000000002], "Focus": [17741.7], "XYStage": [ 10346.1, 77.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos31", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.49], "Focus": [17741.4], "XYStage": [ 9755.5, 85.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos32", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.475], "Focus": [17741.5], "XYStage": [ 4775.5, 293.90000000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos33", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.445], "Focus": [17741.4], "XYStage": [ 3954.9, 293.90000000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos34", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.455], "Focus": [17741.4], "XYStage": [ -1324.9, 545.6 ] }, "GridColumnIndex": 0 }, { "Label": "Pos35", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.445], "Focus": [17741.3], "XYStage": [ -2097, 576.6 ] }, "GridColumnIndex": 0 } ], "z-step_um": 10, "GridRow": 0, "BitDepth": 16, "Depth": 2, "Channels": 6, "Source": "Micro-Manager", "ChColors": [ -16711681, -256, -65281, -1, -1, -16776961 ], "MetadataVersion": 10, "KeepShutterOpenChannels": true, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536, 65536 ], "CustomIntervals_ms": [], "Height": 1200, "MicroManagerVersion": "1.4.23 20180219", "GridColumn": 0, "UUID": "14a13bf7-c944-4672-b873-fcfbbb7ea13d", "PixelType": "GRAY16", "Width": 1200, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\Yan\\20191218-YC113-S7S8-miseq", "Frames": 1, "Comment": "", "UserName": "Nikon", "PixelSize_um": 1.1, "ROI": [ 0, 0, 1200, 1200 ], "Date": "2019-12-18", "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC", "P-DAPI-high" ], "KeepShutterOpenSlices": true, "IJType": 1, "ComputerName": "ZadorConfocal2", "Positions": 36, "PositionIndex": 9, "CameraTimeout": 20000, "TimeFirst": false }, "640 AO-Name": "AnalogIO", "Vincent-D3-Command": "Close" }, "FrameKey-0-4-8": { "COM4-StopBits": "1", "528 AO-MaxVolts": "5.0000", "XLIGHT Excitation Wheel-State": "1", "470 Shutter-Name": "AnalogIO", "Core-Shutter": "Vincent-D3", "555 AO-Demo": "No", "XLIGHT Dichroic-State": "2", "XYStage-StepSize-Y": "0.1000", "ZPositionUm": 278.82, "XYStage-StepSize-X": "0.1000", "PRIME-FirmwareVersion": "20.28", "PRIME-ClearCycles": "2", "520-Name": "DA Shutter", "405 Shutter-Name": "AnalogIO", "PIEZO-StepSize": "0.0050", "PRIME-ClearMode": "Pre-Exposure", "470 AO-MinVolts": "0.0000", "405 AO-SupportsTriggering": "1", "CameraChannelIndex": 0, "XYStage-StartSpeed": "500.0000", "COM9-StopBits": "2", "405 AO-Description": "NI DAC", "COM4-DelayBetweenCharsMs": "0.0000", "445 AO-Block voltage": "false", "520 AO-TriggerInputLine": "/Dev1/PFI0", "555 AO-Description": "NI DAC", "520 AO-TriggerSampleRate": "100000", "COM4-Verbose": "1", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM4-Name": "COM4", "405 Shutter-Volts": "0.0000", "Vincent-D3-Port": "COM1", "PRIME-PP 4 MIN ADU AFFECTED": "200", "470 Shutter-MinVolts": "0.0000", "PRIME-PP 3 ENABLED": "Yes", "470 AO-Description": "NI DAC", "PRIME-Description": "PVCAM API device adapter", "520 Shutter-MaxVolts": "5.0000", "520 Shutter-Volts": "0.0000", "528 AO-Sequenceable": "1", "PRIME-Binning": "1x1", "555 Shutter-MaxVolts": "5.0000", "528 Shutter-TriggerSequenceLength": "1000", "405 Shutter-Block voltage": "false", "XLIGHT Spin motor-State": "1", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "Vincent-D3-Time to open (ms)": "35.0000", "Vincent-D3-Time to close (ms)": "35.0000", "445 AO-TriggerSampleRate": "100000", "640 Shutter-IOChannel": "Dev1/ao14", "555 AO-Sequenceable": "1", "520 AO-Name": "AnalogIO", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "470 AO-Block voltage": "false", "COM1-Verbose": "1", "COM9-AnswerTimeout": "10000.0000", "528 Shutter-Name": "AnalogIO", "PRIME-Trigger Timeout (secs)": "10", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "NextFrame": 0, "470 AO-TriggerSequenceLength": "1000", "PRIME-TransposeXY": "0", "445 AO-TriggerSequenceLength": "1000", "COM1-Name": "COM1", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "COM9-DataBits": "8", "COM1-AnswerTimeout": "500.0000", "Camera": "", "TE2000-Version": "V2.50-V2.50-V2.10", "528 Shutter-Volts": "0.0000", "XLIGHT Hub-Name": "XLIGHT Hub", "PIEZO-Description": "Ludl stage driver adapter", "PositionName": "Pos9", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSampleRate": "100000", "PRIME-FanSpeedSetpoint": "High", "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "405 AO-TriggerSampleRate": "100000", "470 Shutter-Block voltage": "false", "470-State": "0", "COM3-Parity": "None", "405 AO-Volts": "0.0000", "PRIME-TransposeMirrorY": "0", "PRIME-ChipName": "GS144BSI", "470 AO-Volts": "0.0000", "PRIME-TransposeMirrorX": "0", "445 Shutter-SupportsTriggering": "0", "470 Shutter-SupportsTriggering": "0", "XLIGHT Hub-Description": "XLIGHT hub", "PRIME-PreampOffLimit": "10000", "470 AO-Sequenceable": "1", "528 Shutter-MaxVolts": "5.0000", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "Core-XYStage": "XYStage", "470 AO-TriggerSampleRate": "100000", "PRIME-Timing-PreTriggerDelayNs": "24672000.0000", "528-DA Device": "528 Shutter", "Focus-Description": "Nikon TE2000 focus adapter", "FilterBlock-State": "5", "520 AO-Demo": "No", "COM3-DelayBetweenCharsMs": "0.0000", "470 Shutter-Volts": "0.0000", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "PRIME-PP 2 THRESHOLD": "125", "PRIME-PP 4 THRESHOLD": "75", "XYStage-Acceleration": "4.0000", "470 Shutter-Demo": "No", "PRIME-Name": "PMPCIECam00", "640-DA Device": "640 Shutter", "405 AO-Block voltage": "false", "ElapsedTime-ms": 405030, "PRIME-Offset": "100", "445 AO-IOChannel": "Dev1/ao1", "640 AO-MaxVolts": "5.0000", "PositionIndex": 9, "PRIME-PP 1 ENABLED": "Yes", "COM1-BaudRate": "9600", "640-State": "0", "COM3-Verbose": "1", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "LudlController-TransmissionDelay": "0", "PRIME-FullWellCapacity": "62000", "PRIME-SMARTStreamingEnabled": "No", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "640 Shutter-Description": "NI DAC", "528-Description": "DA device that is used as a shutter", "COM4-Description": "Serial port driver (boost:asio)", "405 Shutter-Description": "NI DAC", "TE2000-Name": "TE2000", "528 Shutter-Description": "NI DAC", "COM9-Description": "Serial port driver (boost:asio)", "LudlController-Name": "LudlController", "445 Shutter-TriggerSequenceLength": "1000", "PRIME-SerialNumber": "A18B203008", "445 AO-Volts": "0.0000", "PRIME-X-dimension": "1200", "PRIME-ShutterCloseDelay": "0", "XLIGHT Disk slider-State": "2", "Position": "Default", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 AO-IOChannel": "Dev1/ao5", "520 AO-IOChannel": "Dev1/ao4", "528 AO-TriggerSequenceLength": "1000", "405 Shutter-MaxVolts": "5.0000", "470-DA Device": "470 Shutter", "PRIME-FTCapable": "No", "Core-ChannelGroup": "Channels", "555 AO-SupportsTriggering": "0", "PRIME-Y-dimension": "1200", "Frame": 0, "555 Shutter-Sequenceable": "1", "PRIME-Timing-ExposureTimeNs": "499998000.0000", "Core-ImageProcessor": "", "528-State": "0", "520 AO-Block voltage": "false", "XLIGHT Excitation Wheel-Label": "Excitation-2", "640 Shutter-Demo": "No", "COM3-StopBits": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "520 Shutter-IOChannel": "Dev1/ao11", "Width": 1200, "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-SupportsTriggering": "0", "640 AO-TriggerSampleRate": "100000", "520-Description": "DA device that is used as a shutter", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "405 Shutter-Sequenceable": "1", "405 Shutter-SupportsTriggering": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-TriggerSampleRate": "100000", "640 Shutter-MaxVolts": "5.0000", "555 AO-MaxVolts": "5.0000", "520 AO-TriggerSequenceLength": "1000", "COM3-BaudRate": "9600", "COM9-BaudRate": "9600", "Vincent-D3-Channel #": "Ch. #1", "PRIME-PMode": "Normal", "OpticalPath-Label": "Left(100)", "BitDepth": 16, "555 AO-TriggerSequenceLength": "1000", "445 Shutter-Description": "NI DAC", "405 AO-Sequenceable": "1", "PRIME-AcquisitionMethod": "Callbacks", "555 AO-Name": "AnalogIO", "PRIME-ShutterMode": "Pre-Exposure", "COM3-Handshaking": "Off", "445 AO-SupportsTriggering": "0", "ROI": "0-0-1200-1200", "COM9-Verbose": "1", "PRIME-Color": "OFF", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "PRIME-ShutterOpenDelay": "0", "COM1-Parity": "None", "Slice": 8, "SlicePosition": 30, "COM9-DelayBetweenCharsMs": "0.0000", "555-Name": "DA Shutter", "PIEZO-LudlSingleAxisName": "B", "XLIGHT Emission Wheel-Label": "Empty", "640 Shutter-Name": "AnalogIO", "640 AO-Sequenceable": "1", "520 Shutter-Demo": "No", "528 Shutter-Demo": "No", "470-Name": "DA Shutter", "520-DA Device": "520 Shutter", "XYStage-Description": "Ludl XY stage driver adapter", "445 Shutter-MinVolts": "0.0000", "528 AO-TriggerSampleRate": "100000", "405 AO-MinVolts": "0.0000", "405 Shutter-TriggerSequenceLength": "1000", "Height": 1200, "555 AO-Block voltage": "false", "640 Shutter-Volts": "0.0000", "PixelType": "GRAY16", "445 Shutter-Block voltage": "false", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "520 Shutter-TriggerSequenceLength": "1000", "COM9-Name": "COM9", "Core-SLM": "", "555 AO-Volts": "0.0000", "555 Shutter-Block voltage": "false", "XYStage-Speed": "1800.0000", "520 AO-MaxVolts": "5.0000", "528 Shutter-SupportsTriggering": "0", "COM1-Description": "Serial port driver (boost:asio)", "PRIME-MetadataEnabled": "No", "520 Shutter-MinVolts": "0.0000", "470 Shutter-Sequenceable": "1", "PRIME-PreampDelay": "0", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-MinVolts": "0.0000", "ChannelIndex": 4, "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Core-AutoShutter": "1", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "Exposure-ms": 20, "PRIME-PP 2 ENABLED": "Yes", "640 Shutter-TriggerSampleRate": "100000", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "470 Shutter-IOChannel": "Dev1/ao10", "405 AO-Demo": "No", "PixelSizeUm": 1.1, "470 AO-Demo": "No", "470 Shutter-MaxVolts": "5.0000", "Vincent-D3-Name": "Vincent-D3", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FileName": "img_000000000_P-DIC_008.tif", "LudlController-Reset": "Operate", "PRIME-Exposure": "20.00", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "445-Name": "DA Shutter", "555 Shutter-Name": "AnalogIO", "520 Shutter-TriggerSampleRate": "100000", "445 Shutter-IOChannel": "Dev1/ao9", "PRIME-TransposeCorrection": "0", "XYStage-StepSize": "0.1000", "445-Description": "DA device that is used as a shutter", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 AO-IOChannel": "Dev1/ao7", "520 Shutter-Name": "AnalogIO", "COM3-DataBits": "8", "640 AO-Volts": "0.0000", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "555 Shutter-IOChannel": "Dev1/ao13", "WaitInterval": null, "520 Shutter-Sequenceable": "1", "YPositionUm": 31506.2, "445-State": "0", "445-DA Device": "445 Shutter", "PRIME-CircularBufferAutoSize": "ON", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "555 AO-TriggerSampleRate": "100000", "445 Shutter-Volts": "0.0000", "XLIGHT Hub-Port": "COM3", "Core-AutoFocus": "", "COM4-Handshaking": "Off", "528 Shutter-MinVolts": "0.0000", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "445 Shutter-Name": "AnalogIO", "528 Shutter-Sequenceable": "1", "XYStage-TransposeMirrorX": "0", "XYStage-TransposeMirrorY": "0", "PRIME-ReadoutRate": "100MHz 16bit", "XYStage-Name": "Stage", "640 AO-SupportsTriggering": "0", "640-Name": "DA Shutter", "640 Shutter-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "640 AO-Block voltage": "false", "445 Shutter-Sequenceable": "1", "470 AO-MaxVolts": "5.0000", "COM4-BaudRate": "9600", "470-Description": "DA device that is used as a shutter", "520 Shutter-Description": "NI DAC", "PRIME-CCDTemperature": "-14.8500", "Time": "2019-12-18 21:37:12 -0500", "640 AO-TriggerSequenceLength": "1000", "470 AO-Name": "AnalogIO", "XLIGHT Disk slider-Label": "Disk Pos 40um", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "Binning": "1x1", "TE2000-ModelType": "1", "COM3-AnswerTimeout": "500.0000", "555 Shutter-SupportsTriggering": "0", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "TE2000-Port": "COM4", "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Description": "NI DAC", "COM4-AnswerTimeout": "500.0000", "COM1-DelayBetweenCharsMs": "0.0000", "FilterBlock-Label": "Confocal", "445 AO-Name": "AnalogIO", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-Timing-ReadoutTimeNs": "24672000.0000", "528 AO-Demo": "No", "520 AO-Sequenceable": "1", "555-Description": "DA device that is used as a shutter", "405-DA Device": "405 Shutter", "405 AO-TriggerSequenceLength": "1000", "640 AO-MinVolts": "0.0000", "640 Shutter-Block voltage": "false", "FrameIndex": 0, "PRIME-Port": "CMOS", "555 AO-MinVolts": "0.0000", "COM3-Description": "Serial port driver (boost:asio)", "528 AO-Description": "NI DAC", "555 AO-IOChannel": "Dev1/ao6", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "555 AO-TriggerInputLine": "/Dev1/PFI0", "PRIME-ActualInterval-ms": "390.5200", "LudlController-Port": "COM9", "445 Shutter-Demo": "No", "PRIME-Trigger-Expose Out-Mux": "1", "SliceIndex": 8, "PRIME-CCDTemperatureSetPoint": "-15.0000", "405 Shutter-MinVolts": "0.0000", "PRIME-ExposeOutMode": "First Row", "AxisPositions": null, "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Volts": "0.0000", "528-Name": "DA Shutter", "555-State": "0", "XLIGHT Dichroic-Label": "Di03-R635-t1", "445 AO-Sequenceable": "1", "405-Name": "DA Shutter", "405 AO-IOChannel": "Dev1/ao0", "Core-Camera": "PRIME", "Focus-Name": "Focus", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "405 Shutter-IOChannel": "Dev1/ao8", "640 AO-Demo": "No", "640 Shutter-SupportsTriggering": "0", "445 AO-Demo": "No", "555-DA Device": "555 Shutter", "470 Shutter-Description": "NI DAC", "528 AO-Volts": "0.0000", "COM1-DataBits": "8", "Vincent-D3-Address": "x", "520 AO-SupportsTriggering": "0", "405-Description": "DA device that is used as a shutter", "640 Shutter-MinVolts": "0.0000", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "405 AO-Name": "AnalogIO", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "640 AO-TriggerInputLine": "/Dev1/PFI0", "405 AO-TriggerInputLine": "/Dev1/PFI0", "640-Description": "DA device that is used as a shutter", "445 Shutter-TriggerSampleRate": "100000", "470 Shutter-TriggerSequenceLength": "1000", "COM1-StopBits": "1", "520 Shutter-SupportsTriggering": "0", "405-State": "0", "Core-Initialize": "1", "XLIGHT Emission Wheel-State": "3", "520 AO-Description": "NI DAC", "528 AO-TriggerInputLine": "/Dev1/PFI0", "445 Shutter-MaxVolts": "5.0000", "COM9-Handshaking": "Off", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "XLIGHT Spin motor-Label": "On (spinning)", "PRIME-PP 5 ENABLED": "No", "UUID": "698a9192-6fe2-4b4d-a9e1-a7879710a28f", "PRIME-TriggerMode": "Internal Trigger", "COM1-Handshaking": "Off", "PRIME-Timing-PostTriggerDelayNs": "49344000.0000", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "Focus-Position": "17741.5000", "528 Shutter-Block voltage": "false", "Channel": "P-DIC", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "555 Shutter-Demo": "No", "405 Shutter-Demo": "No", "528 AO-Name": "AnalogIO", "520-State": "0", "Core-Galvo": "", "405 Shutter-TriggerSampleRate": "100000", "Focus-FocusStepSize_(nm)": "100", "PRIME-PixelType": "16bit", "COM4-DataBits": "8", "470 AO-IOChannel": "Dev1/ao2", "520 AO-MinVolts": "0.0000", "COM9-Parity": "None", "PRIME-CircularBufferEnabled": "ON", "PRIME-PP 6 Reset": "No", "640 AO-Description": "NI DAC", "Source": "PRIME", "445 AO-MinVolts": "0.0000", "PIEZO-Name": "Stage", "445 AO-Description": "NI DAC", "PRIME-CircularBufferFrameCount": "46", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "520 AO-Volts": "0.0000", "528 Shutter-TriggerSampleRate": "100000", "XPositionUm": 19731.9, "OpticalPath-State": "4", "PRIME-PP 3 THRESHOLD": "80", "PRIME-PVCAM Version": "3.7.1", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Gain": "1-HDR", "640 Shutter-TriggerSequenceLength": "1000", "528 AO-SupportsTriggering": "0", "445 AO-TriggerInputLine": "/Dev1/PFI0", "OpticalPath-Name": "OpticalPath", "PRIME-PP 4 ENABLED": "Yes", "PRIME-PVCAM Adapter Version": "1.3.29", "COM3-Name": "COM3", "405 AO-MaxVolts": "5.0000", "520 Shutter-Block voltage": "false", "PRIME-Timing-ClearingTimeNs": "49344000.0000", "Core-Focus": "PIEZO", "528 AO-Block voltage": "false", "TE2000-Description": "Nikon TE2000 hub", "528 AO-MinVolts": "0.0000", "COM4-Parity": "None", "Summary": { "Slices": 11, "ChContrastMin": [ 0, 0, 0, 0, 0, 0 ], "PixelAspect": 1, "SlicesFirst": true, "Interval_ms": 0, "Time": "2019-12-18 21:36:42 -0500", "Prefix": "seq_4", "InitialPositionList": [ { "Label": "Pos0", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.21], "Focus": [17741.5], "XYStage": [ 44542.200000000004, 31636.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos1", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.22], "Focus": [17741.4], "XYStage": [ 43801.100000000006, 31637.9 ] }, "GridColumnIndex": 0 }, { "Label": "Pos2", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.5], "XYStage": [ 38561.3, 32040.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos3", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.185], "Focus": [17741.3], "XYStage": [ 37698.4, 32041.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos4", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.16], "Focus": [17741.5], "XYStage": [ 32375.4, 31758.9 ] }, "GridColumnIndex": 0 }, { "Label": "Pos5", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.17000000000002], "Focus": [17741.3], "XYStage": [ 31770.7, 31758.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos6", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.4], "XYStage": [ 26536.800000000003, 31068.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos7", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.205], "Focus": [17741.4], "XYStage": [ 25420.2, 31068.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos8", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.85], "Focus": [17741.3], "XYStage": [ 21059, 31508 ] }, "GridColumnIndex": 0 }, { "Label": "Pos9", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.82], "Focus": [17741.3], "XYStage": [ 19731.9, 31506.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos10", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.79], "Focus": [17741.4], "XYStage": [ 14528.5, 32007.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos11", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.83], "Focus": [17741.2], "XYStage": [ 13830.300000000001, 32009.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos12", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.79], "Focus": [17741.3], "XYStage": [ 8775.300000000001, 32017.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos13", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.815], "Focus": [17741.3], "XYStage": [ 8109.200000000001, 32008.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos14", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.805], "Focus": [17741.3], "XYStage": [ 3483.3, 31908.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos15", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.82], "Focus": [17741.2], "XYStage": [ 2686.8, 31903.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos16", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [211.48000000000002], "Focus": [17741.1], "XYStage": [ -1978.9, 31973.600000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos17", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [211.51500000000001], "Focus": [17741.1], "XYStage": [ -2957.8, 31982.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos18", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.195], "Focus": [17741.5], "XYStage": [ 47747.4, -576.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos19", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.23000000000002], "Focus": [17741.6], "XYStage": [ 47053.9, -576.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos20", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.205], "Focus": [17741.4], "XYStage": [ 41871.200000000004, -479.8 ] }, "GridColumnIndex": 0 }, { "Label": "Pos21", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.4], "XYStage": [ 40882.600000000006, -392 ] }, "GridColumnIndex": 0 }, { "Label": "Pos22", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.24], "Focus": [17741.4], "XYStage": [ 35905.6, -135.3 ] }, "GridColumnIndex": 0 }, { "Label": "Pos23", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.195], "Focus": [17741.5], "XYStage": [ 34990.9, -132.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos24", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.46], "Focus": [17741.4], "XYStage": [ 29678.100000000002, -958.1 ] }, "GridColumnIndex": 0 }, { "Label": "Pos25", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.44], "Focus": [17741.5], "XYStage": [ 28983.600000000002, -939.4000000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos26", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.475], "Focus": [17741.5], "XYStage": [ 23019.7, -122 ] }, "GridColumnIndex": 0 }, { "Label": "Pos27", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.44], "Focus": [17741.7], "XYStage": [ 22428, -116.80000000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos28", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.46], "Focus": [17741.7], "XYStage": [ 16364.900000000001, -181.10000000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos29", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.48000000000002], "Focus": [17741.5], "XYStage": [ 15712.1, -180 ] }, "GridColumnIndex": 0 }, { "Label": "Pos30", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.45000000000002], "Focus": [17741.7], "XYStage": [ 10346.1, 77.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos31", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.49], "Focus": [17741.4], "XYStage": [ 9755.5, 85.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos32", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.475], "Focus": [17741.5], "XYStage": [ 4775.5, 293.90000000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos33", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.445], "Focus": [17741.4], "XYStage": [ 3954.9, 293.90000000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos34", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.455], "Focus": [17741.4], "XYStage": [ -1324.9, 545.6 ] }, "GridColumnIndex": 0 }, { "Label": "Pos35", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.445], "Focus": [17741.3], "XYStage": [ -2097, 576.6 ] }, "GridColumnIndex": 0 } ], "z-step_um": 10, "GridRow": 0, "BitDepth": 16, "Depth": 2, "Channels": 6, "Source": "Micro-Manager", "ChColors": [ -16711681, -256, -65281, -1, -1, -16776961 ], "MetadataVersion": 10, "KeepShutterOpenChannels": true, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536, 65536 ], "CustomIntervals_ms": [], "Height": 1200, "MicroManagerVersion": "1.4.23 20180219", "GridColumn": 0, "UUID": "14a13bf7-c944-4672-b873-fcfbbb7ea13d", "PixelType": "GRAY16", "Width": 1200, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\Yan\\20191218-YC113-S7S8-miseq", "Frames": 1, "Comment": "", "UserName": "Nikon", "PixelSize_um": 1.1, "ROI": [ 0, 0, 1200, 1200 ], "Date": "2019-12-18", "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC", "P-DAPI-high" ], "KeepShutterOpenSlices": true, "IJType": 1, "ComputerName": "ZadorConfocal2", "Positions": 36, "PositionIndex": 9, "CameraTimeout": 20000, "TimeFirst": false }, "640 AO-Name": "AnalogIO", "Vincent-D3-Command": "Close" }, "FrameKey-0-4-9": { "COM4-StopBits": "1", "528 AO-MaxVolts": "5.0000", "XLIGHT Excitation Wheel-State": "1", "470 Shutter-Name": "AnalogIO", "Core-Shutter": "Vincent-D3", "555 AO-Demo": "No", "XLIGHT Dichroic-State": "2", "XYStage-StepSize-Y": "0.1000", "ZPositionUm": 288.82, "XYStage-StepSize-X": "0.1000", "PRIME-FirmwareVersion": "20.28", "PRIME-ClearCycles": "2", "520-Name": "DA Shutter", "405 Shutter-Name": "AnalogIO", "PIEZO-StepSize": "0.0050", "PRIME-ClearMode": "Pre-Exposure", "470 AO-MinVolts": "0.0000", "405 AO-SupportsTriggering": "1", "CameraChannelIndex": 0, "XYStage-StartSpeed": "500.0000", "COM9-StopBits": "2", "405 AO-Description": "NI DAC", "COM4-DelayBetweenCharsMs": "0.0000", "445 AO-Block voltage": "false", "520 AO-TriggerInputLine": "/Dev1/PFI0", "555 AO-Description": "NI DAC", "520 AO-TriggerSampleRate": "100000", "COM4-Verbose": "1", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM4-Name": "COM4", "405 Shutter-Volts": "0.0000", "Vincent-D3-Port": "COM1", "PRIME-PP 4 MIN ADU AFFECTED": "200", "470 Shutter-MinVolts": "0.0000", "PRIME-PP 3 ENABLED": "Yes", "470 AO-Description": "NI DAC", "PRIME-Description": "PVCAM API device adapter", "520 Shutter-MaxVolts": "5.0000", "520 Shutter-Volts": "0.0000", "528 AO-Sequenceable": "1", "PRIME-Binning": "1x1", "555 Shutter-MaxVolts": "5.0000", "528 Shutter-TriggerSequenceLength": "1000", "405 Shutter-Block voltage": "false", "XLIGHT Spin motor-State": "1", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "Vincent-D3-Time to open (ms)": "35.0000", "Vincent-D3-Time to close (ms)": "35.0000", "445 AO-TriggerSampleRate": "100000", "640 Shutter-IOChannel": "Dev1/ao14", "555 AO-Sequenceable": "1", "520 AO-Name": "AnalogIO", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "470 AO-Block voltage": "false", "COM1-Verbose": "1", "COM9-AnswerTimeout": "10000.0000", "528 Shutter-Name": "AnalogIO", "PRIME-Trigger Timeout (secs)": "10", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "NextFrame": 0, "470 AO-TriggerSequenceLength": "1000", "PRIME-TransposeXY": "0", "445 AO-TriggerSequenceLength": "1000", "COM1-Name": "COM1", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "COM9-DataBits": "8", "COM1-AnswerTimeout": "500.0000", "Camera": "", "TE2000-Version": "V2.50-V2.50-V2.10", "528 Shutter-Volts": "0.0000", "XLIGHT Hub-Name": "XLIGHT Hub", "PIEZO-Description": "Ludl stage driver adapter", "PositionName": "Pos9", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSampleRate": "100000", "PRIME-FanSpeedSetpoint": "High", "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "405 AO-TriggerSampleRate": "100000", "470 Shutter-Block voltage": "false", "470-State": "0", "COM3-Parity": "None", "405 AO-Volts": "0.0000", "PRIME-TransposeMirrorY": "0", "PRIME-ChipName": "GS144BSI", "470 AO-Volts": "0.0000", "PRIME-TransposeMirrorX": "0", "445 Shutter-SupportsTriggering": "0", "470 Shutter-SupportsTriggering": "0", "XLIGHT Hub-Description": "XLIGHT hub", "PRIME-PreampOffLimit": "10000", "470 AO-Sequenceable": "1", "528 Shutter-MaxVolts": "5.0000", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "Core-XYStage": "XYStage", "470 AO-TriggerSampleRate": "100000", "PRIME-Timing-PreTriggerDelayNs": "24672000.0000", "528-DA Device": "528 Shutter", "Focus-Description": "Nikon TE2000 focus adapter", "FilterBlock-State": "5", "520 AO-Demo": "No", "COM3-DelayBetweenCharsMs": "0.0000", "470 Shutter-Volts": "0.0000", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "PRIME-PP 2 THRESHOLD": "125", "PRIME-PP 4 THRESHOLD": "75", "XYStage-Acceleration": "4.0000", "470 Shutter-Demo": "No", "PRIME-Name": "PMPCIECam00", "640-DA Device": "640 Shutter", "405 AO-Block voltage": "false", "ElapsedTime-ms": 405288, "PRIME-Offset": "100", "445 AO-IOChannel": "Dev1/ao1", "640 AO-MaxVolts": "5.0000", "PositionIndex": 9, "PRIME-PP 1 ENABLED": "Yes", "COM1-BaudRate": "9600", "640-State": "0", "COM3-Verbose": "1", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "LudlController-TransmissionDelay": "0", "PRIME-FullWellCapacity": "62000", "PRIME-SMARTStreamingEnabled": "No", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "640 Shutter-Description": "NI DAC", "528-Description": "DA device that is used as a shutter", "COM4-Description": "Serial port driver (boost:asio)", "405 Shutter-Description": "NI DAC", "TE2000-Name": "TE2000", "528 Shutter-Description": "NI DAC", "COM9-Description": "Serial port driver (boost:asio)", "LudlController-Name": "LudlController", "445 Shutter-TriggerSequenceLength": "1000", "PRIME-SerialNumber": "A18B203008", "445 AO-Volts": "0.0000", "PRIME-X-dimension": "1200", "PRIME-ShutterCloseDelay": "0", "XLIGHT Disk slider-State": "2", "Position": "Default", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 AO-IOChannel": "Dev1/ao5", "520 AO-IOChannel": "Dev1/ao4", "528 AO-TriggerSequenceLength": "1000", "405 Shutter-MaxVolts": "5.0000", "470-DA Device": "470 Shutter", "PRIME-FTCapable": "No", "Core-ChannelGroup": "Channels", "555 AO-SupportsTriggering": "0", "PRIME-Y-dimension": "1200", "Frame": 0, "555 Shutter-Sequenceable": "1", "PRIME-Timing-ExposureTimeNs": "499998000.0000", "Core-ImageProcessor": "", "528-State": "0", "520 AO-Block voltage": "false", "XLIGHT Excitation Wheel-Label": "Excitation-2", "640 Shutter-Demo": "No", "COM3-StopBits": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "520 Shutter-IOChannel": "Dev1/ao11", "Width": 1200, "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-SupportsTriggering": "0", "640 AO-TriggerSampleRate": "100000", "520-Description": "DA device that is used as a shutter", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "405 Shutter-Sequenceable": "1", "405 Shutter-SupportsTriggering": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-TriggerSampleRate": "100000", "640 Shutter-MaxVolts": "5.0000", "555 AO-MaxVolts": "5.0000", "520 AO-TriggerSequenceLength": "1000", "COM3-BaudRate": "9600", "COM9-BaudRate": "9600", "Vincent-D3-Channel #": "Ch. #1", "PRIME-PMode": "Normal", "OpticalPath-Label": "Left(100)", "BitDepth": 16, "555 AO-TriggerSequenceLength": "1000", "445 Shutter-Description": "NI DAC", "405 AO-Sequenceable": "1", "PRIME-AcquisitionMethod": "Callbacks", "555 AO-Name": "AnalogIO", "PRIME-ShutterMode": "Pre-Exposure", "COM3-Handshaking": "Off", "445 AO-SupportsTriggering": "0", "ROI": "0-0-1200-1200", "COM9-Verbose": "1", "PRIME-Color": "OFF", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "PRIME-ShutterOpenDelay": "0", "COM1-Parity": "None", "Slice": 9, "SlicePosition": 40, "COM9-DelayBetweenCharsMs": "0.0000", "555-Name": "DA Shutter", "PIEZO-LudlSingleAxisName": "B", "XLIGHT Emission Wheel-Label": "Empty", "640 Shutter-Name": "AnalogIO", "640 AO-Sequenceable": "1", "520 Shutter-Demo": "No", "528 Shutter-Demo": "No", "470-Name": "DA Shutter", "520-DA Device": "520 Shutter", "XYStage-Description": "Ludl XY stage driver adapter", "445 Shutter-MinVolts": "0.0000", "528 AO-TriggerSampleRate": "100000", "405 AO-MinVolts": "0.0000", "405 Shutter-TriggerSequenceLength": "1000", "Height": 1200, "555 AO-Block voltage": "false", "640 Shutter-Volts": "0.0000", "PixelType": "GRAY16", "445 Shutter-Block voltage": "false", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "520 Shutter-TriggerSequenceLength": "1000", "COM9-Name": "COM9", "Core-SLM": "", "555 AO-Volts": "0.0000", "555 Shutter-Block voltage": "false", "XYStage-Speed": "1800.0000", "520 AO-MaxVolts": "5.0000", "528 Shutter-SupportsTriggering": "0", "COM1-Description": "Serial port driver (boost:asio)", "PRIME-MetadataEnabled": "No", "520 Shutter-MinVolts": "0.0000", "470 Shutter-Sequenceable": "1", "PRIME-PreampDelay": "0", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-MinVolts": "0.0000", "ChannelIndex": 4, "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Core-AutoShutter": "1", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "Exposure-ms": 20, "PRIME-PP 2 ENABLED": "Yes", "640 Shutter-TriggerSampleRate": "100000", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "470 Shutter-IOChannel": "Dev1/ao10", "405 AO-Demo": "No", "PixelSizeUm": 1.1, "470 AO-Demo": "No", "470 Shutter-MaxVolts": "5.0000", "Vincent-D3-Name": "Vincent-D3", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FileName": "img_000000000_P-DIC_009.tif", "LudlController-Reset": "Operate", "PRIME-Exposure": "20.00", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "445-Name": "DA Shutter", "555 Shutter-Name": "AnalogIO", "520 Shutter-TriggerSampleRate": "100000", "445 Shutter-IOChannel": "Dev1/ao9", "PRIME-TransposeCorrection": "0", "XYStage-StepSize": "0.1000", "445-Description": "DA device that is used as a shutter", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 AO-IOChannel": "Dev1/ao7", "520 Shutter-Name": "AnalogIO", "COM3-DataBits": "8", "640 AO-Volts": "0.0000", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "555 Shutter-IOChannel": "Dev1/ao13", "WaitInterval": null, "520 Shutter-Sequenceable": "1", "YPositionUm": 31506.2, "445-State": "0", "445-DA Device": "445 Shutter", "PRIME-CircularBufferAutoSize": "ON", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "555 AO-TriggerSampleRate": "100000", "445 Shutter-Volts": "0.0000", "XLIGHT Hub-Port": "COM3", "Core-AutoFocus": "", "COM4-Handshaking": "Off", "528 Shutter-MinVolts": "0.0000", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "445 Shutter-Name": "AnalogIO", "528 Shutter-Sequenceable": "1", "XYStage-TransposeMirrorX": "0", "XYStage-TransposeMirrorY": "0", "PRIME-ReadoutRate": "100MHz 16bit", "XYStage-Name": "Stage", "640 AO-SupportsTriggering": "0", "640-Name": "DA Shutter", "640 Shutter-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "640 AO-Block voltage": "false", "445 Shutter-Sequenceable": "1", "470 AO-MaxVolts": "5.0000", "COM4-BaudRate": "9600", "470-Description": "DA device that is used as a shutter", "520 Shutter-Description": "NI DAC", "PRIME-CCDTemperature": "-14.8500", "Time": "2019-12-18 21:37:13 -0500", "640 AO-TriggerSequenceLength": "1000", "470 AO-Name": "AnalogIO", "XLIGHT Disk slider-Label": "Disk Pos 40um", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "Binning": "1x1", "TE2000-ModelType": "1", "COM3-AnswerTimeout": "500.0000", "555 Shutter-SupportsTriggering": "0", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "TE2000-Port": "COM4", "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Description": "NI DAC", "COM4-AnswerTimeout": "500.0000", "COM1-DelayBetweenCharsMs": "0.0000", "FilterBlock-Label": "Confocal", "445 AO-Name": "AnalogIO", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-Timing-ReadoutTimeNs": "24672000.0000", "528 AO-Demo": "No", "520 AO-Sequenceable": "1", "555-Description": "DA device that is used as a shutter", "405-DA Device": "405 Shutter", "405 AO-TriggerSequenceLength": "1000", "640 AO-MinVolts": "0.0000", "640 Shutter-Block voltage": "false", "FrameIndex": 0, "PRIME-Port": "CMOS", "555 AO-MinVolts": "0.0000", "COM3-Description": "Serial port driver (boost:asio)", "528 AO-Description": "NI DAC", "555 AO-IOChannel": "Dev1/ao6", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "555 AO-TriggerInputLine": "/Dev1/PFI0", "PRIME-ActualInterval-ms": "390.5200", "LudlController-Port": "COM9", "445 Shutter-Demo": "No", "PRIME-Trigger-Expose Out-Mux": "1", "SliceIndex": 9, "PRIME-CCDTemperatureSetPoint": "-15.0000", "405 Shutter-MinVolts": "0.0000", "PRIME-ExposeOutMode": "First Row", "AxisPositions": null, "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Volts": "0.0000", "528-Name": "DA Shutter", "555-State": "0", "XLIGHT Dichroic-Label": "Di03-R635-t1", "445 AO-Sequenceable": "1", "405-Name": "DA Shutter", "405 AO-IOChannel": "Dev1/ao0", "Core-Camera": "PRIME", "Focus-Name": "Focus", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "405 Shutter-IOChannel": "Dev1/ao8", "640 AO-Demo": "No", "640 Shutter-SupportsTriggering": "0", "445 AO-Demo": "No", "555-DA Device": "555 Shutter", "470 Shutter-Description": "NI DAC", "528 AO-Volts": "0.0000", "COM1-DataBits": "8", "Vincent-D3-Address": "x", "520 AO-SupportsTriggering": "0", "405-Description": "DA device that is used as a shutter", "640 Shutter-MinVolts": "0.0000", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "405 AO-Name": "AnalogIO", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "640 AO-TriggerInputLine": "/Dev1/PFI0", "405 AO-TriggerInputLine": "/Dev1/PFI0", "640-Description": "DA device that is used as a shutter", "445 Shutter-TriggerSampleRate": "100000", "470 Shutter-TriggerSequenceLength": "1000", "COM1-StopBits": "1", "520 Shutter-SupportsTriggering": "0", "405-State": "0", "Core-Initialize": "1", "XLIGHT Emission Wheel-State": "3", "520 AO-Description": "NI DAC", "528 AO-TriggerInputLine": "/Dev1/PFI0", "445 Shutter-MaxVolts": "5.0000", "COM9-Handshaking": "Off", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "XLIGHT Spin motor-Label": "On (spinning)", "PRIME-PP 5 ENABLED": "No", "UUID": "6ec4e50a-5a4f-4673-b3a8-8fb630907635", "PRIME-TriggerMode": "Internal Trigger", "COM1-Handshaking": "Off", "PRIME-Timing-PostTriggerDelayNs": "49344000.0000", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "Focus-Position": "17741.5000", "528 Shutter-Block voltage": "false", "Channel": "P-DIC", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "555 Shutter-Demo": "No", "405 Shutter-Demo": "No", "528 AO-Name": "AnalogIO", "520-State": "0", "Core-Galvo": "", "405 Shutter-TriggerSampleRate": "100000", "Focus-FocusStepSize_(nm)": "100", "PRIME-PixelType": "16bit", "COM4-DataBits": "8", "470 AO-IOChannel": "Dev1/ao2", "520 AO-MinVolts": "0.0000", "COM9-Parity": "None", "PRIME-CircularBufferEnabled": "ON", "PRIME-PP 6 Reset": "No", "640 AO-Description": "NI DAC", "Source": "PRIME", "445 AO-MinVolts": "0.0000", "PIEZO-Name": "Stage", "445 AO-Description": "NI DAC", "PRIME-CircularBufferFrameCount": "46", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "520 AO-Volts": "0.0000", "528 Shutter-TriggerSampleRate": "100000", "XPositionUm": 19731.9, "OpticalPath-State": "4", "PRIME-PP 3 THRESHOLD": "80", "PRIME-PVCAM Version": "3.7.1", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Gain": "1-HDR", "640 Shutter-TriggerSequenceLength": "1000", "528 AO-SupportsTriggering": "0", "445 AO-TriggerInputLine": "/Dev1/PFI0", "OpticalPath-Name": "OpticalPath", "PRIME-PP 4 ENABLED": "Yes", "PRIME-PVCAM Adapter Version": "1.3.29", "COM3-Name": "COM3", "405 AO-MaxVolts": "5.0000", "520 Shutter-Block voltage": "false", "PRIME-Timing-ClearingTimeNs": "49344000.0000", "Core-Focus": "PIEZO", "528 AO-Block voltage": "false", "TE2000-Description": "Nikon TE2000 hub", "528 AO-MinVolts": "0.0000", "COM4-Parity": "None", "Summary": { "Slices": 11, "ChContrastMin": [ 0, 0, 0, 0, 0, 0 ], "PixelAspect": 1, "SlicesFirst": true, "Interval_ms": 0, "Time": "2019-12-18 21:36:42 -0500", "Prefix": "seq_4", "InitialPositionList": [ { "Label": "Pos0", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.21], "Focus": [17741.5], "XYStage": [ 44542.200000000004, 31636.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos1", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.22], "Focus": [17741.4], "XYStage": [ 43801.100000000006, 31637.9 ] }, "GridColumnIndex": 0 }, { "Label": "Pos2", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.5], "XYStage": [ 38561.3, 32040.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos3", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.185], "Focus": [17741.3], "XYStage": [ 37698.4, 32041.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos4", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.16], "Focus": [17741.5], "XYStage": [ 32375.4, 31758.9 ] }, "GridColumnIndex": 0 }, { "Label": "Pos5", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.17000000000002], "Focus": [17741.3], "XYStage": [ 31770.7, 31758.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos6", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.4], "XYStage": [ 26536.800000000003, 31068.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos7", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.205], "Focus": [17741.4], "XYStage": [ 25420.2, 31068.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos8", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.85], "Focus": [17741.3], "XYStage": [ 21059, 31508 ] }, "GridColumnIndex": 0 }, { "Label": "Pos9", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.82], "Focus": [17741.3], "XYStage": [ 19731.9, 31506.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos10", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.79], "Focus": [17741.4], "XYStage": [ 14528.5, 32007.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos11", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.83], "Focus": [17741.2], "XYStage": [ 13830.300000000001, 32009.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos12", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.79], "Focus": [17741.3], "XYStage": [ 8775.300000000001, 32017.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos13", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.815], "Focus": [17741.3], "XYStage": [ 8109.200000000001, 32008.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos14", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.805], "Focus": [17741.3], "XYStage": [ 3483.3, 31908.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos15", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.82], "Focus": [17741.2], "XYStage": [ 2686.8, 31903.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos16", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [211.48000000000002], "Focus": [17741.1], "XYStage": [ -1978.9, 31973.600000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos17", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [211.51500000000001], "Focus": [17741.1], "XYStage": [ -2957.8, 31982.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos18", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.195], "Focus": [17741.5], "XYStage": [ 47747.4, -576.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos19", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.23000000000002], "Focus": [17741.6], "XYStage": [ 47053.9, -576.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos20", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.205], "Focus": [17741.4], "XYStage": [ 41871.200000000004, -479.8 ] }, "GridColumnIndex": 0 }, { "Label": "Pos21", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.4], "XYStage": [ 40882.600000000006, -392 ] }, "GridColumnIndex": 0 }, { "Label": "Pos22", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.24], "Focus": [17741.4], "XYStage": [ 35905.6, -135.3 ] }, "GridColumnIndex": 0 }, { "Label": "Pos23", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.195], "Focus": [17741.5], "XYStage": [ 34990.9, -132.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos24", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.46], "Focus": [17741.4], "XYStage": [ 29678.100000000002, -958.1 ] }, "GridColumnIndex": 0 }, { "Label": "Pos25", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.44], "Focus": [17741.5], "XYStage": [ 28983.600000000002, -939.4000000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos26", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.475], "Focus": [17741.5], "XYStage": [ 23019.7, -122 ] }, "GridColumnIndex": 0 }, { "Label": "Pos27", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.44], "Focus": [17741.7], "XYStage": [ 22428, -116.80000000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos28", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.46], "Focus": [17741.7], "XYStage": [ 16364.900000000001, -181.10000000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos29", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.48000000000002], "Focus": [17741.5], "XYStage": [ 15712.1, -180 ] }, "GridColumnIndex": 0 }, { "Label": "Pos30", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.45000000000002], "Focus": [17741.7], "XYStage": [ 10346.1, 77.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos31", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.49], "Focus": [17741.4], "XYStage": [ 9755.5, 85.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos32", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.475], "Focus": [17741.5], "XYStage": [ 4775.5, 293.90000000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos33", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.445], "Focus": [17741.4], "XYStage": [ 3954.9, 293.90000000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos34", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.455], "Focus": [17741.4], "XYStage": [ -1324.9, 545.6 ] }, "GridColumnIndex": 0 }, { "Label": "Pos35", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.445], "Focus": [17741.3], "XYStage": [ -2097, 576.6 ] }, "GridColumnIndex": 0 } ], "z-step_um": 10, "GridRow": 0, "BitDepth": 16, "Depth": 2, "Channels": 6, "Source": "Micro-Manager", "ChColors": [ -16711681, -256, -65281, -1, -1, -16776961 ], "MetadataVersion": 10, "KeepShutterOpenChannels": true, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536, 65536 ], "CustomIntervals_ms": [], "Height": 1200, "MicroManagerVersion": "1.4.23 20180219", "GridColumn": 0, "UUID": "14a13bf7-c944-4672-b873-fcfbbb7ea13d", "PixelType": "GRAY16", "Width": 1200, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\Yan\\20191218-YC113-S7S8-miseq", "Frames": 1, "Comment": "", "UserName": "Nikon", "PixelSize_um": 1.1, "ROI": [ 0, 0, 1200, 1200 ], "Date": "2019-12-18", "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC", "P-DAPI-high" ], "KeepShutterOpenSlices": true, "IJType": 1, "ComputerName": "ZadorConfocal2", "Positions": 36, "PositionIndex": 9, "CameraTimeout": 20000, "TimeFirst": false }, "640 AO-Name": "AnalogIO", "Vincent-D3-Command": "Close" }, "FrameKey-0-4-10": { "COM4-StopBits": "1", "528 AO-MaxVolts": "5.0000", "XLIGHT Excitation Wheel-State": "1", "470 Shutter-Name": "AnalogIO", "Core-Shutter": "Vincent-D3", "555 AO-Demo": "No", "XLIGHT Dichroic-State": "2", "XYStage-StepSize-Y": "0.1000", "ZPositionUm": 298.82, "XYStage-StepSize-X": "0.1000", "PRIME-FirmwareVersion": "20.28", "PRIME-ClearCycles": "2", "520-Name": "DA Shutter", "405 Shutter-Name": "AnalogIO", "PIEZO-StepSize": "0.0050", "PRIME-ClearMode": "Pre-Exposure", "470 AO-MinVolts": "0.0000", "405 AO-SupportsTriggering": "1", "CameraChannelIndex": 0, "XYStage-StartSpeed": "500.0000", "COM9-StopBits": "2", "405 AO-Description": "NI DAC", "COM4-DelayBetweenCharsMs": "0.0000", "445 AO-Block voltage": "false", "520 AO-TriggerInputLine": "/Dev1/PFI0", "555 AO-Description": "NI DAC", "520 AO-TriggerSampleRate": "100000", "COM4-Verbose": "1", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM4-Name": "COM4", "405 Shutter-Volts": "0.0000", "Vincent-D3-Port": "COM1", "PRIME-PP 4 MIN ADU AFFECTED": "200", "470 Shutter-MinVolts": "0.0000", "PRIME-PP 3 ENABLED": "Yes", "470 AO-Description": "NI DAC", "PRIME-Description": "PVCAM API device adapter", "520 Shutter-MaxVolts": "5.0000", "520 Shutter-Volts": "0.0000", "528 AO-Sequenceable": "1", "PRIME-Binning": "1x1", "555 Shutter-MaxVolts": "5.0000", "528 Shutter-TriggerSequenceLength": "1000", "405 Shutter-Block voltage": "false", "XLIGHT Spin motor-State": "1", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "Vincent-D3-Time to open (ms)": "35.0000", "Vincent-D3-Time to close (ms)": "35.0000", "445 AO-TriggerSampleRate": "100000", "640 Shutter-IOChannel": "Dev1/ao14", "555 AO-Sequenceable": "1", "520 AO-Name": "AnalogIO", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "470 AO-Block voltage": "false", "COM1-Verbose": "1", "COM9-AnswerTimeout": "10000.0000", "528 Shutter-Name": "AnalogIO", "PRIME-Trigger Timeout (secs)": "10", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "NextFrame": 0, "470 AO-TriggerSequenceLength": "1000", "PRIME-TransposeXY": "0", "445 AO-TriggerSequenceLength": "1000", "COM1-Name": "COM1", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "COM9-DataBits": "8", "COM1-AnswerTimeout": "500.0000", "Camera": "", "TE2000-Version": "V2.50-V2.50-V2.10", "528 Shutter-Volts": "0.0000", "XLIGHT Hub-Name": "XLIGHT Hub", "PIEZO-Description": "Ludl stage driver adapter", "PositionName": "Pos9", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSampleRate": "100000", "PRIME-FanSpeedSetpoint": "High", "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "405 AO-TriggerSampleRate": "100000", "470 Shutter-Block voltage": "false", "470-State": "0", "COM3-Parity": "None", "405 AO-Volts": "0.0000", "PRIME-TransposeMirrorY": "0", "PRIME-ChipName": "GS144BSI", "470 AO-Volts": "0.0000", "PRIME-TransposeMirrorX": "0", "445 Shutter-SupportsTriggering": "0", "470 Shutter-SupportsTriggering": "0", "XLIGHT Hub-Description": "XLIGHT hub", "PRIME-PreampOffLimit": "10000", "470 AO-Sequenceable": "1", "528 Shutter-MaxVolts": "5.0000", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "Core-XYStage": "XYStage", "470 AO-TriggerSampleRate": "100000", "PRIME-Timing-PreTriggerDelayNs": "24672000.0000", "528-DA Device": "528 Shutter", "Focus-Description": "Nikon TE2000 focus adapter", "FilterBlock-State": "5", "520 AO-Demo": "No", "COM3-DelayBetweenCharsMs": "0.0000", "470 Shutter-Volts": "0.0000", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "PRIME-PP 2 THRESHOLD": "125", "PRIME-PP 4 THRESHOLD": "75", "XYStage-Acceleration": "4.0000", "470 Shutter-Demo": "No", "PRIME-Name": "PMPCIECam00", "640-DA Device": "640 Shutter", "405 AO-Block voltage": "false", "ElapsedTime-ms": 405596, "PRIME-Offset": "100", "445 AO-IOChannel": "Dev1/ao1", "640 AO-MaxVolts": "5.0000", "PositionIndex": 9, "PRIME-PP 1 ENABLED": "Yes", "COM1-BaudRate": "9600", "640-State": "0", "COM3-Verbose": "1", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "LudlController-TransmissionDelay": "0", "PRIME-FullWellCapacity": "62000", "PRIME-SMARTStreamingEnabled": "No", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "640 Shutter-Description": "NI DAC", "528-Description": "DA device that is used as a shutter", "COM4-Description": "Serial port driver (boost:asio)", "405 Shutter-Description": "NI DAC", "TE2000-Name": "TE2000", "528 Shutter-Description": "NI DAC", "COM9-Description": "Serial port driver (boost:asio)", "LudlController-Name": "LudlController", "445 Shutter-TriggerSequenceLength": "1000", "PRIME-SerialNumber": "A18B203008", "445 AO-Volts": "0.0000", "PRIME-X-dimension": "1200", "PRIME-ShutterCloseDelay": "0", "XLIGHT Disk slider-State": "2", "Position": "Default", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 AO-IOChannel": "Dev1/ao5", "520 AO-IOChannel": "Dev1/ao4", "528 AO-TriggerSequenceLength": "1000", "405 Shutter-MaxVolts": "5.0000", "470-DA Device": "470 Shutter", "PRIME-FTCapable": "No", "Core-ChannelGroup": "Channels", "555 AO-SupportsTriggering": "0", "PRIME-Y-dimension": "1200", "Frame": 0, "555 Shutter-Sequenceable": "1", "PRIME-Timing-ExposureTimeNs": "499998000.0000", "Core-ImageProcessor": "", "528-State": "0", "520 AO-Block voltage": "false", "XLIGHT Excitation Wheel-Label": "Excitation-2", "640 Shutter-Demo": "No", "COM3-StopBits": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "520 Shutter-IOChannel": "Dev1/ao11", "Width": 1200, "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-SupportsTriggering": "0", "640 AO-TriggerSampleRate": "100000", "520-Description": "DA device that is used as a shutter", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "405 Shutter-Sequenceable": "1", "405 Shutter-SupportsTriggering": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-TriggerSampleRate": "100000", "640 Shutter-MaxVolts": "5.0000", "555 AO-MaxVolts": "5.0000", "520 AO-TriggerSequenceLength": "1000", "COM3-BaudRate": "9600", "COM9-BaudRate": "9600", "Vincent-D3-Channel #": "Ch. #1", "PRIME-PMode": "Normal", "OpticalPath-Label": "Left(100)", "BitDepth": 16, "555 AO-TriggerSequenceLength": "1000", "445 Shutter-Description": "NI DAC", "405 AO-Sequenceable": "1", "PRIME-AcquisitionMethod": "Callbacks", "555 AO-Name": "AnalogIO", "PRIME-ShutterMode": "Pre-Exposure", "COM3-Handshaking": "Off", "445 AO-SupportsTriggering": "0", "ROI": "0-0-1200-1200", "COM9-Verbose": "1", "PRIME-Color": "OFF", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "PRIME-ShutterOpenDelay": "0", "COM1-Parity": "None", "Slice": 10, "SlicePosition": 50, "COM9-DelayBetweenCharsMs": "0.0000", "555-Name": "DA Shutter", "PIEZO-LudlSingleAxisName": "B", "XLIGHT Emission Wheel-Label": "Empty", "640 Shutter-Name": "AnalogIO", "640 AO-Sequenceable": "1", "520 Shutter-Demo": "No", "528 Shutter-Demo": "No", "470-Name": "DA Shutter", "520-DA Device": "520 Shutter", "XYStage-Description": "Ludl XY stage driver adapter", "445 Shutter-MinVolts": "0.0000", "528 AO-TriggerSampleRate": "100000", "405 AO-MinVolts": "0.0000", "405 Shutter-TriggerSequenceLength": "1000", "Height": 1200, "555 AO-Block voltage": "false", "640 Shutter-Volts": "0.0000", "PixelType": "GRAY16", "445 Shutter-Block voltage": "false", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "520 Shutter-TriggerSequenceLength": "1000", "COM9-Name": "COM9", "Core-SLM": "", "555 AO-Volts": "0.0000", "555 Shutter-Block voltage": "false", "XYStage-Speed": "1800.0000", "520 AO-MaxVolts": "5.0000", "528 Shutter-SupportsTriggering": "0", "COM1-Description": "Serial port driver (boost:asio)", "PRIME-MetadataEnabled": "No", "520 Shutter-MinVolts": "0.0000", "470 Shutter-Sequenceable": "1", "PRIME-PreampDelay": "0", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-MinVolts": "0.0000", "ChannelIndex": 4, "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Core-AutoShutter": "1", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "Exposure-ms": 20, "PRIME-PP 2 ENABLED": "Yes", "640 Shutter-TriggerSampleRate": "100000", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "470 Shutter-IOChannel": "Dev1/ao10", "405 AO-Demo": "No", "PixelSizeUm": 1.1, "470 AO-Demo": "No", "470 Shutter-MaxVolts": "5.0000", "Vincent-D3-Name": "Vincent-D3", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FileName": "img_000000000_P-DIC_010.tif", "LudlController-Reset": "Operate", "PRIME-Exposure": "20.00", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "445-Name": "DA Shutter", "555 Shutter-Name": "AnalogIO", "520 Shutter-TriggerSampleRate": "100000", "445 Shutter-IOChannel": "Dev1/ao9", "PRIME-TransposeCorrection": "0", "XYStage-StepSize": "0.1000", "445-Description": "DA device that is used as a shutter", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 AO-IOChannel": "Dev1/ao7", "520 Shutter-Name": "AnalogIO", "COM3-DataBits": "8", "640 AO-Volts": "0.0000", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "555 Shutter-IOChannel": "Dev1/ao13", "WaitInterval": null, "520 Shutter-Sequenceable": "1", "YPositionUm": 31506.2, "445-State": "0", "445-DA Device": "445 Shutter", "PRIME-CircularBufferAutoSize": "ON", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "555 AO-TriggerSampleRate": "100000", "445 Shutter-Volts": "0.0000", "XLIGHT Hub-Port": "COM3", "Core-AutoFocus": "", "COM4-Handshaking": "Off", "528 Shutter-MinVolts": "0.0000", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "445 Shutter-Name": "AnalogIO", "528 Shutter-Sequenceable": "1", "XYStage-TransposeMirrorX": "0", "XYStage-TransposeMirrorY": "0", "PRIME-ReadoutRate": "100MHz 16bit", "XYStage-Name": "Stage", "640 AO-SupportsTriggering": "0", "640-Name": "DA Shutter", "640 Shutter-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "640 AO-Block voltage": "false", "445 Shutter-Sequenceable": "1", "470 AO-MaxVolts": "5.0000", "COM4-BaudRate": "9600", "470-Description": "DA device that is used as a shutter", "520 Shutter-Description": "NI DAC", "PRIME-CCDTemperature": "-14.8500", "Time": "2019-12-18 21:37:13 -0500", "640 AO-TriggerSequenceLength": "1000", "470 AO-Name": "AnalogIO", "XLIGHT Disk slider-Label": "Disk Pos 40um", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "Binning": "1x1", "TE2000-ModelType": "1", "COM3-AnswerTimeout": "500.0000", "555 Shutter-SupportsTriggering": "0", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "TE2000-Port": "COM4", "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Description": "NI DAC", "COM4-AnswerTimeout": "500.0000", "COM1-DelayBetweenCharsMs": "0.0000", "FilterBlock-Label": "Confocal", "445 AO-Name": "AnalogIO", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-Timing-ReadoutTimeNs": "24672000.0000", "528 AO-Demo": "No", "520 AO-Sequenceable": "1", "555-Description": "DA device that is used as a shutter", "405-DA Device": "405 Shutter", "405 AO-TriggerSequenceLength": "1000", "640 AO-MinVolts": "0.0000", "640 Shutter-Block voltage": "false", "FrameIndex": 0, "PRIME-Port": "CMOS", "555 AO-MinVolts": "0.0000", "COM3-Description": "Serial port driver (boost:asio)", "528 AO-Description": "NI DAC", "555 AO-IOChannel": "Dev1/ao6", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "555 AO-TriggerInputLine": "/Dev1/PFI0", "PRIME-ActualInterval-ms": "390.5200", "LudlController-Port": "COM9", "445 Shutter-Demo": "No", "PRIME-Trigger-Expose Out-Mux": "1", "SliceIndex": 10, "PRIME-CCDTemperatureSetPoint": "-15.0000", "405 Shutter-MinVolts": "0.0000", "PRIME-ExposeOutMode": "First Row", "AxisPositions": null, "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Volts": "0.0000", "528-Name": "DA Shutter", "555-State": "0", "XLIGHT Dichroic-Label": "Di03-R635-t1", "445 AO-Sequenceable": "1", "405-Name": "DA Shutter", "405 AO-IOChannel": "Dev1/ao0", "Core-Camera": "PRIME", "Focus-Name": "Focus", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "405 Shutter-IOChannel": "Dev1/ao8", "640 AO-Demo": "No", "640 Shutter-SupportsTriggering": "0", "445 AO-Demo": "No", "555-DA Device": "555 Shutter", "470 Shutter-Description": "NI DAC", "528 AO-Volts": "0.0000", "COM1-DataBits": "8", "Vincent-D3-Address": "x", "520 AO-SupportsTriggering": "0", "405-Description": "DA device that is used as a shutter", "640 Shutter-MinVolts": "0.0000", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "405 AO-Name": "AnalogIO", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "640 AO-TriggerInputLine": "/Dev1/PFI0", "405 AO-TriggerInputLine": "/Dev1/PFI0", "640-Description": "DA device that is used as a shutter", "445 Shutter-TriggerSampleRate": "100000", "470 Shutter-TriggerSequenceLength": "1000", "COM1-StopBits": "1", "520 Shutter-SupportsTriggering": "0", "405-State": "0", "Core-Initialize": "1", "XLIGHT Emission Wheel-State": "3", "520 AO-Description": "NI DAC", "528 AO-TriggerInputLine": "/Dev1/PFI0", "445 Shutter-MaxVolts": "5.0000", "COM9-Handshaking": "Off", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "XLIGHT Spin motor-Label": "On (spinning)", "PRIME-PP 5 ENABLED": "No", "UUID": "ecee5e2a-7cba-4a6b-acb4-105fe3c9435c", "PRIME-TriggerMode": "Internal Trigger", "COM1-Handshaking": "Off", "PRIME-Timing-PostTriggerDelayNs": "49344000.0000", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "Focus-Position": "17741.5000", "528 Shutter-Block voltage": "false", "Channel": "P-DIC", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "555 Shutter-Demo": "No", "405 Shutter-Demo": "No", "528 AO-Name": "AnalogIO", "520-State": "0", "Core-Galvo": "", "405 Shutter-TriggerSampleRate": "100000", "Focus-FocusStepSize_(nm)": "100", "PRIME-PixelType": "16bit", "COM4-DataBits": "8", "470 AO-IOChannel": "Dev1/ao2", "520 AO-MinVolts": "0.0000", "COM9-Parity": "None", "PRIME-CircularBufferEnabled": "ON", "PRIME-PP 6 Reset": "No", "640 AO-Description": "NI DAC", "Source": "PRIME", "445 AO-MinVolts": "0.0000", "PIEZO-Name": "Stage", "445 AO-Description": "NI DAC", "PRIME-CircularBufferFrameCount": "46", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "520 AO-Volts": "0.0000", "528 Shutter-TriggerSampleRate": "100000", "XPositionUm": 19731.9, "OpticalPath-State": "4", "PRIME-PP 3 THRESHOLD": "80", "PRIME-PVCAM Version": "3.7.1", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Gain": "1-HDR", "640 Shutter-TriggerSequenceLength": "1000", "528 AO-SupportsTriggering": "0", "445 AO-TriggerInputLine": "/Dev1/PFI0", "OpticalPath-Name": "OpticalPath", "PRIME-PP 4 ENABLED": "Yes", "PRIME-PVCAM Adapter Version": "1.3.29", "COM3-Name": "COM3", "405 AO-MaxVolts": "5.0000", "520 Shutter-Block voltage": "false", "PRIME-Timing-ClearingTimeNs": "49344000.0000", "Core-Focus": "PIEZO", "528 AO-Block voltage": "false", "TE2000-Description": "Nikon TE2000 hub", "528 AO-MinVolts": "0.0000", "COM4-Parity": "None", "Summary": { "Slices": 11, "ChContrastMin": [ 0, 0, 0, 0, 0, 0 ], "PixelAspect": 1, "SlicesFirst": true, "Interval_ms": 0, "Time": "2019-12-18 21:36:42 -0500", "Prefix": "seq_4", "InitialPositionList": [ { "Label": "Pos0", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.21], "Focus": [17741.5], "XYStage": [ 44542.200000000004, 31636.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos1", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.22], "Focus": [17741.4], "XYStage": [ 43801.100000000006, 31637.9 ] }, "GridColumnIndex": 0 }, { "Label": "Pos2", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.5], "XYStage": [ 38561.3, 32040.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos3", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.185], "Focus": [17741.3], "XYStage": [ 37698.4, 32041.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos4", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.16], "Focus": [17741.5], "XYStage": [ 32375.4, 31758.9 ] }, "GridColumnIndex": 0 }, { "Label": "Pos5", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.17000000000002], "Focus": [17741.3], "XYStage": [ 31770.7, 31758.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos6", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.4], "XYStage": [ 26536.800000000003, 31068.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos7", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.205], "Focus": [17741.4], "XYStage": [ 25420.2, 31068.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos8", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.85], "Focus": [17741.3], "XYStage": [ 21059, 31508 ] }, "GridColumnIndex": 0 }, { "Label": "Pos9", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.82], "Focus": [17741.3], "XYStage": [ 19731.9, 31506.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos10", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.79], "Focus": [17741.4], "XYStage": [ 14528.5, 32007.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos11", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.83], "Focus": [17741.2], "XYStage": [ 13830.300000000001, 32009.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos12", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.79], "Focus": [17741.3], "XYStage": [ 8775.300000000001, 32017.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos13", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.815], "Focus": [17741.3], "XYStage": [ 8109.200000000001, 32008.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos14", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.805], "Focus": [17741.3], "XYStage": [ 3483.3, 31908.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos15", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.82], "Focus": [17741.2], "XYStage": [ 2686.8, 31903.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos16", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [211.48000000000002], "Focus": [17741.1], "XYStage": [ -1978.9, 31973.600000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos17", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [211.51500000000001], "Focus": [17741.1], "XYStage": [ -2957.8, 31982.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos18", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.195], "Focus": [17741.5], "XYStage": [ 47747.4, -576.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos19", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.23000000000002], "Focus": [17741.6], "XYStage": [ 47053.9, -576.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos20", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.205], "Focus": [17741.4], "XYStage": [ 41871.200000000004, -479.8 ] }, "GridColumnIndex": 0 }, { "Label": "Pos21", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.4], "XYStage": [ 40882.600000000006, -392 ] }, "GridColumnIndex": 0 }, { "Label": "Pos22", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.24], "Focus": [17741.4], "XYStage": [ 35905.6, -135.3 ] }, "GridColumnIndex": 0 }, { "Label": "Pos23", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.195], "Focus": [17741.5], "XYStage": [ 34990.9, -132.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos24", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.46], "Focus": [17741.4], "XYStage": [ 29678.100000000002, -958.1 ] }, "GridColumnIndex": 0 }, { "Label": "Pos25", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.44], "Focus": [17741.5], "XYStage": [ 28983.600000000002, -939.4000000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos26", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.475], "Focus": [17741.5], "XYStage": [ 23019.7, -122 ] }, "GridColumnIndex": 0 }, { "Label": "Pos27", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.44], "Focus": [17741.7], "XYStage": [ 22428, -116.80000000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos28", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.46], "Focus": [17741.7], "XYStage": [ 16364.900000000001, -181.10000000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos29", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.48000000000002], "Focus": [17741.5], "XYStage": [ 15712.1, -180 ] }, "GridColumnIndex": 0 }, { "Label": "Pos30", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.45000000000002], "Focus": [17741.7], "XYStage": [ 10346.1, 77.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos31", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.49], "Focus": [17741.4], "XYStage": [ 9755.5, 85.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos32", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.475], "Focus": [17741.5], "XYStage": [ 4775.5, 293.90000000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos33", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.445], "Focus": [17741.4], "XYStage": [ 3954.9, 293.90000000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos34", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.455], "Focus": [17741.4], "XYStage": [ -1324.9, 545.6 ] }, "GridColumnIndex": 0 }, { "Label": "Pos35", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.445], "Focus": [17741.3], "XYStage": [ -2097, 576.6 ] }, "GridColumnIndex": 0 } ], "z-step_um": 10, "GridRow": 0, "BitDepth": 16, "Depth": 2, "Channels": 6, "Source": "Micro-Manager", "ChColors": [ -16711681, -256, -65281, -1, -1, -16776961 ], "MetadataVersion": 10, "KeepShutterOpenChannels": true, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536, 65536 ], "CustomIntervals_ms": [], "Height": 1200, "MicroManagerVersion": "1.4.23 20180219", "GridColumn": 0, "UUID": "14a13bf7-c944-4672-b873-fcfbbb7ea13d", "PixelType": "GRAY16", "Width": 1200, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\Yan\\20191218-YC113-S7S8-miseq", "Frames": 1, "Comment": "", "UserName": "Nikon", "PixelSize_um": 1.1, "ROI": [ 0, 0, 1200, 1200 ], "Date": "2019-12-18", "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC", "P-DAPI-high" ], "KeepShutterOpenSlices": true, "IJType": 1, "ComputerName": "ZadorConfocal2", "Positions": 36, "PositionIndex": 9, "CameraTimeout": 20000, "TimeFirst": false }, "640 AO-Name": "AnalogIO", "Vincent-D3-Command": "Close" }, "FrameKey-0-5-0": { "COM4-StopBits": "1", "528 AO-MaxVolts": "5.0000", "XLIGHT Excitation Wheel-State": "1", "470 Shutter-Name": "AnalogIO", "Core-Shutter": "405", "555 AO-Demo": "No", "XLIGHT Dichroic-State": "2", "XYStage-StepSize-Y": "0.1000", "ZPositionUm": 198.82, "XYStage-StepSize-X": "0.1000", "PRIME-FirmwareVersion": "20.28", "PRIME-ClearCycles": "2", "520-Name": "DA Shutter", "405 Shutter-Name": "AnalogIO", "PIEZO-StepSize": "0.0050", "PRIME-ClearMode": "Pre-Exposure", "470 AO-MinVolts": "0.0000", "405 AO-SupportsTriggering": "1", "CameraChannelIndex": 0, "XYStage-StartSpeed": "500.0000", "COM9-StopBits": "2", "405 AO-Description": "NI DAC", "COM4-DelayBetweenCharsMs": "0.0000", "445 AO-Block voltage": "false", "520 AO-TriggerInputLine": "/Dev1/PFI0", "555 AO-Description": "NI DAC", "520 AO-TriggerSampleRate": "100000", "COM4-Verbose": "1", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM4-Name": "COM4", "405 Shutter-Volts": "5.0000", "Vincent-D3-Port": "COM1", "PRIME-PP 4 MIN ADU AFFECTED": "200", "470 Shutter-MinVolts": "0.0000", "PRIME-PP 3 ENABLED": "Yes", "470 AO-Description": "NI DAC", "PRIME-Description": "PVCAM API device adapter", "520 Shutter-MaxVolts": "5.0000", "520 Shutter-Volts": "0.0000", "528 AO-Sequenceable": "1", "PRIME-Binning": "1x1", "555 Shutter-MaxVolts": "5.0000", "528 Shutter-TriggerSequenceLength": "1000", "405 Shutter-Block voltage": "false", "XLIGHT Spin motor-State": "1", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "Vincent-D3-Time to open (ms)": "35.0000", "Vincent-D3-Time to close (ms)": "35.0000", "445 AO-TriggerSampleRate": "100000", "640 Shutter-IOChannel": "Dev1/ao14", "555 AO-Sequenceable": "1", "520 AO-Name": "AnalogIO", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "470 AO-Block voltage": "false", "COM1-Verbose": "1", "COM9-AnswerTimeout": "10000.0000", "528 Shutter-Name": "AnalogIO", "PRIME-Trigger Timeout (secs)": "10", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "NextFrame": 0, "470 AO-TriggerSequenceLength": "1000", "PRIME-TransposeXY": "0", "445 AO-TriggerSequenceLength": "1000", "COM1-Name": "COM1", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "COM9-DataBits": "8", "COM1-AnswerTimeout": "500.0000", "Camera": "", "TE2000-Version": "V2.50-V2.50-V2.10", "528 Shutter-Volts": "0.0000", "XLIGHT Hub-Name": "XLIGHT Hub", "PIEZO-Description": "Ludl stage driver adapter", "PositionName": "Pos9", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSampleRate": "100000", "PRIME-FanSpeedSetpoint": "High", "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "405 AO-TriggerSampleRate": "100000", "470 Shutter-Block voltage": "false", "470-State": "0", "COM3-Parity": "None", "405 AO-Volts": "4.5000", "PRIME-TransposeMirrorY": "0", "PRIME-ChipName": "GS144BSI", "470 AO-Volts": "0.0000", "PRIME-TransposeMirrorX": "0", "445 Shutter-SupportsTriggering": "0", "470 Shutter-SupportsTriggering": "0", "XLIGHT Hub-Description": "XLIGHT hub", "PRIME-PreampOffLimit": "10000", "470 AO-Sequenceable": "1", "528 Shutter-MaxVolts": "5.0000", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "Core-XYStage": "XYStage", "470 AO-TriggerSampleRate": "100000", "PRIME-Timing-PreTriggerDelayNs": "24672000.0000", "528-DA Device": "528 Shutter", "Focus-Description": "Nikon TE2000 focus adapter", "FilterBlock-State": "5", "520 AO-Demo": "No", "COM3-DelayBetweenCharsMs": "0.0000", "470 Shutter-Volts": "0.0000", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "PRIME-PP 2 THRESHOLD": "125", "PRIME-PP 4 THRESHOLD": "75", "XYStage-Acceleration": "4.0000", "470 Shutter-Demo": "No", "PRIME-Name": "PMPCIECam00", "640-DA Device": "640 Shutter", "405 AO-Block voltage": "false", "ElapsedTime-ms": 406921, "PRIME-Offset": "100", "445 AO-IOChannel": "Dev1/ao1", "640 AO-MaxVolts": "5.0000", "PositionIndex": 9, "PRIME-PP 1 ENABLED": "Yes", "COM1-BaudRate": "9600", "640-State": "0", "COM3-Verbose": "1", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "LudlController-TransmissionDelay": "0", "PRIME-FullWellCapacity": "62000", "PRIME-SMARTStreamingEnabled": "No", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "640 Shutter-Description": "NI DAC", "528-Description": "DA device that is used as a shutter", "COM4-Description": "Serial port driver (boost:asio)", "405 Shutter-Description": "NI DAC", "TE2000-Name": "TE2000", "528 Shutter-Description": "NI DAC", "COM9-Description": "Serial port driver (boost:asio)", "LudlController-Name": "LudlController", "445 Shutter-TriggerSequenceLength": "1000", "PRIME-SerialNumber": "A18B203008", "445 AO-Volts": "0.0000", "PRIME-X-dimension": "1200", "PRIME-ShutterCloseDelay": "0", "XLIGHT Disk slider-State": "2", "Position": "Default", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 AO-IOChannel": "Dev1/ao5", "520 AO-IOChannel": "Dev1/ao4", "528 AO-TriggerSequenceLength": "1000", "405 Shutter-MaxVolts": "5.0000", "470-DA Device": "470 Shutter", "PRIME-FTCapable": "No", "Core-ChannelGroup": "Channels", "555 AO-SupportsTriggering": "0", "PRIME-Y-dimension": "1200", "Frame": 0, "555 Shutter-Sequenceable": "1", "PRIME-Timing-ExposureTimeNs": "499998000.0000", "Core-ImageProcessor": "", "528-State": "0", "520 AO-Block voltage": "false", "XLIGHT Excitation Wheel-Label": "QUAD", "640 Shutter-Demo": "No", "COM3-StopBits": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "520 Shutter-IOChannel": "Dev1/ao11", "Width": 1200, "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-SupportsTriggering": "0", "640 AO-TriggerSampleRate": "100000", "520-Description": "DA device that is used as a shutter", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "405 Shutter-Sequenceable": "1", "405 Shutter-SupportsTriggering": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-TriggerSampleRate": "100000", "640 Shutter-MaxVolts": "5.0000", "555 AO-MaxVolts": "5.0000", "520 AO-TriggerSequenceLength": "1000", "COM3-BaudRate": "9600", "COM9-BaudRate": "9600", "Vincent-D3-Channel #": "Ch. #1", "PRIME-PMode": "Normal", "OpticalPath-Label": "Left(100)", "BitDepth": 16, "555 AO-TriggerSequenceLength": "1000", "445 Shutter-Description": "NI DAC", "405 AO-Sequenceable": "1", "PRIME-AcquisitionMethod": "Callbacks", "555 AO-Name": "AnalogIO", "PRIME-ShutterMode": "Pre-Exposure", "COM3-Handshaking": "Off", "445 AO-SupportsTriggering": "0", "ROI": "0-0-1200-1200", "COM9-Verbose": "1", "PRIME-Color": "OFF", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "PRIME-ShutterOpenDelay": "0", "COM1-Parity": "None", "Slice": 0, "SlicePosition": -50, "COM9-DelayBetweenCharsMs": "0.0000", "555-Name": "DA Shutter", "PIEZO-LudlSingleAxisName": "B", "XLIGHT Emission Wheel-Label": "QUAD", "640 Shutter-Name": "AnalogIO", "640 AO-Sequenceable": "1", "520 Shutter-Demo": "No", "528 Shutter-Demo": "No", "470-Name": "DA Shutter", "520-DA Device": "520 Shutter", "XYStage-Description": "Ludl XY stage driver adapter", "445 Shutter-MinVolts": "0.0000", "528 AO-TriggerSampleRate": "100000", "405 AO-MinVolts": "0.0000", "405 Shutter-TriggerSequenceLength": "1000", "Height": 1200, "555 AO-Block voltage": "false", "640 Shutter-Volts": "0.0000", "PixelType": "GRAY16", "445 Shutter-Block voltage": "false", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "520 Shutter-TriggerSequenceLength": "1000", "COM9-Name": "COM9", "Core-SLM": "", "555 AO-Volts": "0.0000", "555 Shutter-Block voltage": "false", "XYStage-Speed": "1800.0000", "520 AO-MaxVolts": "5.0000", "528 Shutter-SupportsTriggering": "0", "COM1-Description": "Serial port driver (boost:asio)", "PRIME-MetadataEnabled": "No", "520 Shutter-MinVolts": "0.0000", "470 Shutter-Sequenceable": "1", "PRIME-PreampDelay": "0", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-MinVolts": "0.0000", "ChannelIndex": 5, "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Core-AutoShutter": "1", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "Exposure-ms": 300, "PRIME-PP 2 ENABLED": "Yes", "640 Shutter-TriggerSampleRate": "100000", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "470 Shutter-IOChannel": "Dev1/ao10", "405 AO-Demo": "No", "PixelSizeUm": 1.1, "470 AO-Demo": "No", "470 Shutter-MaxVolts": "5.0000", "Vincent-D3-Name": "Vincent-D3", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FileName": "img_000000000_P-DAPI-high_000.tif", "LudlController-Reset": "Operate", "PRIME-Exposure": "300.00", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "445-Name": "DA Shutter", "555 Shutter-Name": "AnalogIO", "520 Shutter-TriggerSampleRate": "100000", "445 Shutter-IOChannel": "Dev1/ao9", "PRIME-TransposeCorrection": "0", "XYStage-StepSize": "0.1000", "445-Description": "DA device that is used as a shutter", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 AO-IOChannel": "Dev1/ao7", "520 Shutter-Name": "AnalogIO", "COM3-DataBits": "8", "640 AO-Volts": "0.0000", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "555 Shutter-IOChannel": "Dev1/ao13", "WaitInterval": null, "520 Shutter-Sequenceable": "1", "YPositionUm": 31506.2, "445-State": "0", "445-DA Device": "445 Shutter", "PRIME-CircularBufferAutoSize": "ON", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "555 AO-TriggerSampleRate": "100000", "445 Shutter-Volts": "0.0000", "XLIGHT Hub-Port": "COM3", "Core-AutoFocus": "", "COM4-Handshaking": "Off", "528 Shutter-MinVolts": "0.0000", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "445 Shutter-Name": "AnalogIO", "528 Shutter-Sequenceable": "1", "XYStage-TransposeMirrorX": "0", "XYStage-TransposeMirrorY": "0", "PRIME-ReadoutRate": "100MHz 16bit", "XYStage-Name": "Stage", "640 AO-SupportsTriggering": "0", "640-Name": "DA Shutter", "640 Shutter-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "640 AO-Block voltage": "false", "445 Shutter-Sequenceable": "1", "470 AO-MaxVolts": "5.0000", "COM4-BaudRate": "9600", "470-Description": "DA device that is used as a shutter", "520 Shutter-Description": "NI DAC", "PRIME-CCDTemperature": "-14.8500", "Time": "2019-12-18 21:37:14 -0500", "640 AO-TriggerSequenceLength": "1000", "470 AO-Name": "AnalogIO", "XLIGHT Disk slider-Label": "Disk Pos 40um", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "Binning": "1x1", "TE2000-ModelType": "1", "COM3-AnswerTimeout": "500.0000", "555 Shutter-SupportsTriggering": "0", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "TE2000-Port": "COM4", "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Description": "NI DAC", "COM4-AnswerTimeout": "500.0000", "COM1-DelayBetweenCharsMs": "0.0000", "FilterBlock-Label": "Confocal", "445 AO-Name": "AnalogIO", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-Timing-ReadoutTimeNs": "24672000.0000", "528 AO-Demo": "No", "520 AO-Sequenceable": "1", "555-Description": "DA device that is used as a shutter", "405-DA Device": "405 Shutter", "405 AO-TriggerSequenceLength": "1000", "640 AO-MinVolts": "0.0000", "640 Shutter-Block voltage": "false", "FrameIndex": 0, "PRIME-Port": "CMOS", "555 AO-MinVolts": "0.0000", "COM3-Description": "Serial port driver (boost:asio)", "528 AO-Description": "NI DAC", "555 AO-IOChannel": "Dev1/ao6", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "555 AO-TriggerInputLine": "/Dev1/PFI0", "PRIME-ActualInterval-ms": "390.5200", "LudlController-Port": "COM9", "445 Shutter-Demo": "No", "PRIME-Trigger-Expose Out-Mux": "1", "SliceIndex": 0, "PRIME-CCDTemperatureSetPoint": "-15.0000", "405 Shutter-MinVolts": "0.0000", "PRIME-ExposeOutMode": "First Row", "AxisPositions": null, "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Volts": "0.0000", "528-Name": "DA Shutter", "555-State": "0", "XLIGHT Dichroic-Label": "QUAD", "445 AO-Sequenceable": "1", "405-Name": "DA Shutter", "405 AO-IOChannel": "Dev1/ao0", "Core-Camera": "PRIME", "Focus-Name": "Focus", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "405 Shutter-IOChannel": "Dev1/ao8", "640 AO-Demo": "No", "640 Shutter-SupportsTriggering": "0", "445 AO-Demo": "No", "555-DA Device": "555 Shutter", "470 Shutter-Description": "NI DAC", "528 AO-Volts": "0.0000", "COM1-DataBits": "8", "Vincent-D3-Address": "x", "520 AO-SupportsTriggering": "0", "405-Description": "DA device that is used as a shutter", "640 Shutter-MinVolts": "0.0000", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "405 AO-Name": "AnalogIO", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "640 AO-TriggerInputLine": "/Dev1/PFI0", "405 AO-TriggerInputLine": "/Dev1/PFI0", "640-Description": "DA device that is used as a shutter", "445 Shutter-TriggerSampleRate": "100000", "470 Shutter-TriggerSequenceLength": "1000", "COM1-StopBits": "1", "520 Shutter-SupportsTriggering": "0", "405-State": "1", "Core-Initialize": "1", "XLIGHT Emission Wheel-State": "3", "520 AO-Description": "NI DAC", "528 AO-TriggerInputLine": "/Dev1/PFI0", "445 Shutter-MaxVolts": "5.0000", "COM9-Handshaking": "Off", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "XLIGHT Spin motor-Label": "On (spinning)", "PRIME-PP 5 ENABLED": "No", "UUID": "5dae7063-d20f-45cd-a9d6-4c3d3343ed1a", "PRIME-TriggerMode": "Internal Trigger", "COM1-Handshaking": "Off", "PRIME-Timing-PostTriggerDelayNs": "49344000.0000", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "Focus-Position": "17741.5000", "528 Shutter-Block voltage": "false", "Channel": "P-DAPI-high", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "555 Shutter-Demo": "No", "405 Shutter-Demo": "No", "528 AO-Name": "AnalogIO", "520-State": "0", "Core-Galvo": "", "405 Shutter-TriggerSampleRate": "100000", "Focus-FocusStepSize_(nm)": "100", "PRIME-PixelType": "16bit", "COM4-DataBits": "8", "470 AO-IOChannel": "Dev1/ao2", "520 AO-MinVolts": "0.0000", "COM9-Parity": "None", "PRIME-CircularBufferEnabled": "ON", "PRIME-PP 6 Reset": "No", "640 AO-Description": "NI DAC", "Source": "PRIME", "445 AO-MinVolts": "0.0000", "PIEZO-Name": "Stage", "445 AO-Description": "NI DAC", "PRIME-CircularBufferFrameCount": "46", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "520 AO-Volts": "0.0000", "528 Shutter-TriggerSampleRate": "100000", "XPositionUm": 19731.9, "OpticalPath-State": "4", "PRIME-PP 3 THRESHOLD": "80", "PRIME-PVCAM Version": "3.7.1", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Gain": "1-HDR", "640 Shutter-TriggerSequenceLength": "1000", "528 AO-SupportsTriggering": "0", "445 AO-TriggerInputLine": "/Dev1/PFI0", "OpticalPath-Name": "OpticalPath", "PRIME-PP 4 ENABLED": "Yes", "PRIME-PVCAM Adapter Version": "1.3.29", "COM3-Name": "COM3", "405 AO-MaxVolts": "5.0000", "520 Shutter-Block voltage": "false", "PRIME-Timing-ClearingTimeNs": "49344000.0000", "Core-Focus": "PIEZO", "528 AO-Block voltage": "false", "TE2000-Description": "Nikon TE2000 hub", "528 AO-MinVolts": "0.0000", "COM4-Parity": "None", "Summary": { "Slices": 11, "ChContrastMin": [ 0, 0, 0, 0, 0, 0 ], "PixelAspect": 1, "SlicesFirst": true, "Interval_ms": 0, "Time": "2019-12-18 21:36:42 -0500", "Prefix": "seq_4", "InitialPositionList": [ { "Label": "Pos0", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.21], "Focus": [17741.5], "XYStage": [ 44542.200000000004, 31636.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos1", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.22], "Focus": [17741.4], "XYStage": [ 43801.100000000006, 31637.9 ] }, "GridColumnIndex": 0 }, { "Label": "Pos2", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.5], "XYStage": [ 38561.3, 32040.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos3", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.185], "Focus": [17741.3], "XYStage": [ 37698.4, 32041.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos4", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.16], "Focus": [17741.5], "XYStage": [ 32375.4, 31758.9 ] }, "GridColumnIndex": 0 }, { "Label": "Pos5", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.17000000000002], "Focus": [17741.3], "XYStage": [ 31770.7, 31758.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos6", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.4], "XYStage": [ 26536.800000000003, 31068.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos7", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.205], "Focus": [17741.4], "XYStage": [ 25420.2, 31068.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos8", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.85], "Focus": [17741.3], "XYStage": [ 21059, 31508 ] }, "GridColumnIndex": 0 }, { "Label": "Pos9", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.82], "Focus": [17741.3], "XYStage": [ 19731.9, 31506.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos10", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.79], "Focus": [17741.4], "XYStage": [ 14528.5, 32007.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos11", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.83], "Focus": [17741.2], "XYStage": [ 13830.300000000001, 32009.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos12", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.79], "Focus": [17741.3], "XYStage": [ 8775.300000000001, 32017.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos13", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.815], "Focus": [17741.3], "XYStage": [ 8109.200000000001, 32008.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos14", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.805], "Focus": [17741.3], "XYStage": [ 3483.3, 31908.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos15", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.82], "Focus": [17741.2], "XYStage": [ 2686.8, 31903.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos16", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [211.48000000000002], "Focus": [17741.1], "XYStage": [ -1978.9, 31973.600000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos17", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [211.51500000000001], "Focus": [17741.1], "XYStage": [ -2957.8, 31982.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos18", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.195], "Focus": [17741.5], "XYStage": [ 47747.4, -576.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos19", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.23000000000002], "Focus": [17741.6], "XYStage": [ 47053.9, -576.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos20", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.205], "Focus": [17741.4], "XYStage": [ 41871.200000000004, -479.8 ] }, "GridColumnIndex": 0 }, { "Label": "Pos21", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.4], "XYStage": [ 40882.600000000006, -392 ] }, "GridColumnIndex": 0 }, { "Label": "Pos22", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.24], "Focus": [17741.4], "XYStage": [ 35905.6, -135.3 ] }, "GridColumnIndex": 0 }, { "Label": "Pos23", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.195], "Focus": [17741.5], "XYStage": [ 34990.9, -132.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos24", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.46], "Focus": [17741.4], "XYStage": [ 29678.100000000002, -958.1 ] }, "GridColumnIndex": 0 }, { "Label": "Pos25", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.44], "Focus": [17741.5], "XYStage": [ 28983.600000000002, -939.4000000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos26", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.475], "Focus": [17741.5], "XYStage": [ 23019.7, -122 ] }, "GridColumnIndex": 0 }, { "Label": "Pos27", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.44], "Focus": [17741.7], "XYStage": [ 22428, -116.80000000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos28", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.46], "Focus": [17741.7], "XYStage": [ 16364.900000000001, -181.10000000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos29", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.48000000000002], "Focus": [17741.5], "XYStage": [ 15712.1, -180 ] }, "GridColumnIndex": 0 }, { "Label": "Pos30", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.45000000000002], "Focus": [17741.7], "XYStage": [ 10346.1, 77.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos31", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.49], "Focus": [17741.4], "XYStage": [ 9755.5, 85.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos32", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.475], "Focus": [17741.5], "XYStage": [ 4775.5, 293.90000000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos33", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.445], "Focus": [17741.4], "XYStage": [ 3954.9, 293.90000000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos34", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.455], "Focus": [17741.4], "XYStage": [ -1324.9, 545.6 ] }, "GridColumnIndex": 0 }, { "Label": "Pos35", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.445], "Focus": [17741.3], "XYStage": [ -2097, 576.6 ] }, "GridColumnIndex": 0 } ], "z-step_um": 10, "GridRow": 0, "BitDepth": 16, "Depth": 2, "Channels": 6, "Source": "Micro-Manager", "ChColors": [ -16711681, -256, -65281, -1, -1, -16776961 ], "MetadataVersion": 10, "KeepShutterOpenChannels": true, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536, 65536 ], "CustomIntervals_ms": [], "Height": 1200, "MicroManagerVersion": "1.4.23 20180219", "GridColumn": 0, "UUID": "14a13bf7-c944-4672-b873-fcfbbb7ea13d", "PixelType": "GRAY16", "Width": 1200, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\Yan\\20191218-YC113-S7S8-miseq", "Frames": 1, "Comment": "", "UserName": "Nikon", "PixelSize_um": 1.1, "ROI": [ 0, 0, 1200, 1200 ], "Date": "2019-12-18", "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC", "P-DAPI-high" ], "KeepShutterOpenSlices": true, "IJType": 1, "ComputerName": "ZadorConfocal2", "Positions": 36, "PositionIndex": 9, "CameraTimeout": 20000, "TimeFirst": false }, "640 AO-Name": "AnalogIO", "Vincent-D3-Command": "Close" }, "FrameKey-0-5-1": { "COM4-StopBits": "1", "528 AO-MaxVolts": "5.0000", "XLIGHT Excitation Wheel-State": "1", "470 Shutter-Name": "AnalogIO", "Core-Shutter": "405", "555 AO-Demo": "No", "XLIGHT Dichroic-State": "2", "XYStage-StepSize-Y": "0.1000", "ZPositionUm": 208.82, "XYStage-StepSize-X": "0.1000", "PRIME-FirmwareVersion": "20.28", "PRIME-ClearCycles": "2", "520-Name": "DA Shutter", "405 Shutter-Name": "AnalogIO", "PIEZO-StepSize": "0.0050", "PRIME-ClearMode": "Pre-Exposure", "470 AO-MinVolts": "0.0000", "405 AO-SupportsTriggering": "1", "CameraChannelIndex": 0, "XYStage-StartSpeed": "500.0000", "COM9-StopBits": "2", "405 AO-Description": "NI DAC", "COM4-DelayBetweenCharsMs": "0.0000", "445 AO-Block voltage": "false", "520 AO-TriggerInputLine": "/Dev1/PFI0", "555 AO-Description": "NI DAC", "520 AO-TriggerSampleRate": "100000", "COM4-Verbose": "1", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM4-Name": "COM4", "405 Shutter-Volts": "5.0000", "Vincent-D3-Port": "COM1", "PRIME-PP 4 MIN ADU AFFECTED": "200", "470 Shutter-MinVolts": "0.0000", "PRIME-PP 3 ENABLED": "Yes", "470 AO-Description": "NI DAC", "PRIME-Description": "PVCAM API device adapter", "520 Shutter-MaxVolts": "5.0000", "520 Shutter-Volts": "0.0000", "528 AO-Sequenceable": "1", "PRIME-Binning": "1x1", "555 Shutter-MaxVolts": "5.0000", "528 Shutter-TriggerSequenceLength": "1000", "405 Shutter-Block voltage": "false", "XLIGHT Spin motor-State": "1", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "Vincent-D3-Time to open (ms)": "35.0000", "Vincent-D3-Time to close (ms)": "35.0000", "445 AO-TriggerSampleRate": "100000", "640 Shutter-IOChannel": "Dev1/ao14", "555 AO-Sequenceable": "1", "520 AO-Name": "AnalogIO", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "470 AO-Block voltage": "false", "COM1-Verbose": "1", "COM9-AnswerTimeout": "10000.0000", "528 Shutter-Name": "AnalogIO", "PRIME-Trigger Timeout (secs)": "10", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "NextFrame": 0, "470 AO-TriggerSequenceLength": "1000", "PRIME-TransposeXY": "0", "445 AO-TriggerSequenceLength": "1000", "COM1-Name": "COM1", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "COM9-DataBits": "8", "COM1-AnswerTimeout": "500.0000", "Camera": "", "TE2000-Version": "V2.50-V2.50-V2.10", "528 Shutter-Volts": "0.0000", "XLIGHT Hub-Name": "XLIGHT Hub", "PIEZO-Description": "Ludl stage driver adapter", "PositionName": "Pos9", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSampleRate": "100000", "PRIME-FanSpeedSetpoint": "High", "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "405 AO-TriggerSampleRate": "100000", "470 Shutter-Block voltage": "false", "470-State": "0", "COM3-Parity": "None", "405 AO-Volts": "4.5000", "PRIME-TransposeMirrorY": "0", "PRIME-ChipName": "GS144BSI", "470 AO-Volts": "0.0000", "PRIME-TransposeMirrorX": "0", "445 Shutter-SupportsTriggering": "0", "470 Shutter-SupportsTriggering": "0", "XLIGHT Hub-Description": "XLIGHT hub", "PRIME-PreampOffLimit": "10000", "470 AO-Sequenceable": "1", "528 Shutter-MaxVolts": "5.0000", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "Core-XYStage": "XYStage", "470 AO-TriggerSampleRate": "100000", "PRIME-Timing-PreTriggerDelayNs": "24672000.0000", "528-DA Device": "528 Shutter", "Focus-Description": "Nikon TE2000 focus adapter", "FilterBlock-State": "5", "520 AO-Demo": "No", "COM3-DelayBetweenCharsMs": "0.0000", "470 Shutter-Volts": "0.0000", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "PRIME-PP 2 THRESHOLD": "125", "PRIME-PP 4 THRESHOLD": "75", "XYStage-Acceleration": "4.0000", "470 Shutter-Demo": "No", "PRIME-Name": "PMPCIECam00", "640-DA Device": "640 Shutter", "405 AO-Block voltage": "false", "ElapsedTime-ms": 407503, "PRIME-Offset": "100", "445 AO-IOChannel": "Dev1/ao1", "640 AO-MaxVolts": "5.0000", "PositionIndex": 9, "PRIME-PP 1 ENABLED": "Yes", "COM1-BaudRate": "9600", "640-State": "0", "COM3-Verbose": "1", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "LudlController-TransmissionDelay": "0", "PRIME-FullWellCapacity": "62000", "PRIME-SMARTStreamingEnabled": "No", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "640 Shutter-Description": "NI DAC", "528-Description": "DA device that is used as a shutter", "COM4-Description": "Serial port driver (boost:asio)", "405 Shutter-Description": "NI DAC", "TE2000-Name": "TE2000", "528 Shutter-Description": "NI DAC", "COM9-Description": "Serial port driver (boost:asio)", "LudlController-Name": "LudlController", "445 Shutter-TriggerSequenceLength": "1000", "PRIME-SerialNumber": "A18B203008", "445 AO-Volts": "0.0000", "PRIME-X-dimension": "1200", "PRIME-ShutterCloseDelay": "0", "XLIGHT Disk slider-State": "2", "Position": "Default", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 AO-IOChannel": "Dev1/ao5", "520 AO-IOChannel": "Dev1/ao4", "528 AO-TriggerSequenceLength": "1000", "405 Shutter-MaxVolts": "5.0000", "470-DA Device": "470 Shutter", "PRIME-FTCapable": "No", "Core-ChannelGroup": "Channels", "555 AO-SupportsTriggering": "0", "PRIME-Y-dimension": "1200", "Frame": 0, "555 Shutter-Sequenceable": "1", "PRIME-Timing-ExposureTimeNs": "499998000.0000", "Core-ImageProcessor": "", "528-State": "0", "520 AO-Block voltage": "false", "XLIGHT Excitation Wheel-Label": "QUAD", "640 Shutter-Demo": "No", "COM3-StopBits": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "520 Shutter-IOChannel": "Dev1/ao11", "Width": 1200, "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-SupportsTriggering": "0", "640 AO-TriggerSampleRate": "100000", "520-Description": "DA device that is used as a shutter", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "405 Shutter-Sequenceable": "1", "405 Shutter-SupportsTriggering": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-TriggerSampleRate": "100000", "640 Shutter-MaxVolts": "5.0000", "555 AO-MaxVolts": "5.0000", "520 AO-TriggerSequenceLength": "1000", "COM3-BaudRate": "9600", "COM9-BaudRate": "9600", "Vincent-D3-Channel #": "Ch. #1", "PRIME-PMode": "Normal", "OpticalPath-Label": "Left(100)", "BitDepth": 16, "555 AO-TriggerSequenceLength": "1000", "445 Shutter-Description": "NI DAC", "405 AO-Sequenceable": "1", "PRIME-AcquisitionMethod": "Callbacks", "555 AO-Name": "AnalogIO", "PRIME-ShutterMode": "Pre-Exposure", "COM3-Handshaking": "Off", "445 AO-SupportsTriggering": "0", "ROI": "0-0-1200-1200", "COM9-Verbose": "1", "PRIME-Color": "OFF", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "PRIME-ShutterOpenDelay": "0", "COM1-Parity": "None", "Slice": 1, "SlicePosition": -40, "COM9-DelayBetweenCharsMs": "0.0000", "555-Name": "DA Shutter", "PIEZO-LudlSingleAxisName": "B", "XLIGHT Emission Wheel-Label": "QUAD", "640 Shutter-Name": "AnalogIO", "640 AO-Sequenceable": "1", "520 Shutter-Demo": "No", "528 Shutter-Demo": "No", "470-Name": "DA Shutter", "520-DA Device": "520 Shutter", "XYStage-Description": "Ludl XY stage driver adapter", "445 Shutter-MinVolts": "0.0000", "528 AO-TriggerSampleRate": "100000", "405 AO-MinVolts": "0.0000", "405 Shutter-TriggerSequenceLength": "1000", "Height": 1200, "555 AO-Block voltage": "false", "640 Shutter-Volts": "0.0000", "PixelType": "GRAY16", "445 Shutter-Block voltage": "false", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "520 Shutter-TriggerSequenceLength": "1000", "COM9-Name": "COM9", "Core-SLM": "", "555 AO-Volts": "0.0000", "555 Shutter-Block voltage": "false", "XYStage-Speed": "1800.0000", "520 AO-MaxVolts": "5.0000", "528 Shutter-SupportsTriggering": "0", "COM1-Description": "Serial port driver (boost:asio)", "PRIME-MetadataEnabled": "No", "520 Shutter-MinVolts": "0.0000", "470 Shutter-Sequenceable": "1", "PRIME-PreampDelay": "0", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-MinVolts": "0.0000", "ChannelIndex": 5, "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Core-AutoShutter": "1", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "Exposure-ms": 300, "PRIME-PP 2 ENABLED": "Yes", "640 Shutter-TriggerSampleRate": "100000", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "470 Shutter-IOChannel": "Dev1/ao10", "405 AO-Demo": "No", "PixelSizeUm": 1.1, "470 AO-Demo": "No", "470 Shutter-MaxVolts": "5.0000", "Vincent-D3-Name": "Vincent-D3", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FileName": "img_000000000_P-DAPI-high_001.tif", "LudlController-Reset": "Operate", "PRIME-Exposure": "300.00", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "445-Name": "DA Shutter", "555 Shutter-Name": "AnalogIO", "520 Shutter-TriggerSampleRate": "100000", "445 Shutter-IOChannel": "Dev1/ao9", "PRIME-TransposeCorrection": "0", "XYStage-StepSize": "0.1000", "445-Description": "DA device that is used as a shutter", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 AO-IOChannel": "Dev1/ao7", "520 Shutter-Name": "AnalogIO", "COM3-DataBits": "8", "640 AO-Volts": "0.0000", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "555 Shutter-IOChannel": "Dev1/ao13", "WaitInterval": null, "520 Shutter-Sequenceable": "1", "YPositionUm": 31506.2, "445-State": "0", "445-DA Device": "445 Shutter", "PRIME-CircularBufferAutoSize": "ON", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "555 AO-TriggerSampleRate": "100000", "445 Shutter-Volts": "0.0000", "XLIGHT Hub-Port": "COM3", "Core-AutoFocus": "", "COM4-Handshaking": "Off", "528 Shutter-MinVolts": "0.0000", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "445 Shutter-Name": "AnalogIO", "528 Shutter-Sequenceable": "1", "XYStage-TransposeMirrorX": "0", "XYStage-TransposeMirrorY": "0", "PRIME-ReadoutRate": "100MHz 16bit", "XYStage-Name": "Stage", "640 AO-SupportsTriggering": "0", "640-Name": "DA Shutter", "640 Shutter-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "640 AO-Block voltage": "false", "445 Shutter-Sequenceable": "1", "470 AO-MaxVolts": "5.0000", "COM4-BaudRate": "9600", "470-Description": "DA device that is used as a shutter", "520 Shutter-Description": "NI DAC", "PRIME-CCDTemperature": "-14.8500", "Time": "2019-12-18 21:37:15 -0500", "640 AO-TriggerSequenceLength": "1000", "470 AO-Name": "AnalogIO", "XLIGHT Disk slider-Label": "Disk Pos 40um", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "Binning": "1x1", "TE2000-ModelType": "1", "COM3-AnswerTimeout": "500.0000", "555 Shutter-SupportsTriggering": "0", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "TE2000-Port": "COM4", "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Description": "NI DAC", "COM4-AnswerTimeout": "500.0000", "COM1-DelayBetweenCharsMs": "0.0000", "FilterBlock-Label": "Confocal", "445 AO-Name": "AnalogIO", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-Timing-ReadoutTimeNs": "24672000.0000", "528 AO-Demo": "No", "520 AO-Sequenceable": "1", "555-Description": "DA device that is used as a shutter", "405-DA Device": "405 Shutter", "405 AO-TriggerSequenceLength": "1000", "640 AO-MinVolts": "0.0000", "640 Shutter-Block voltage": "false", "FrameIndex": 0, "PRIME-Port": "CMOS", "555 AO-MinVolts": "0.0000", "COM3-Description": "Serial port driver (boost:asio)", "528 AO-Description": "NI DAC", "555 AO-IOChannel": "Dev1/ao6", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "555 AO-TriggerInputLine": "/Dev1/PFI0", "PRIME-ActualInterval-ms": "390.5200", "LudlController-Port": "COM9", "445 Shutter-Demo": "No", "PRIME-Trigger-Expose Out-Mux": "1", "SliceIndex": 1, "PRIME-CCDTemperatureSetPoint": "-15.0000", "405 Shutter-MinVolts": "0.0000", "PRIME-ExposeOutMode": "First Row", "AxisPositions": null, "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Volts": "0.0000", "528-Name": "DA Shutter", "555-State": "0", "XLIGHT Dichroic-Label": "QUAD", "445 AO-Sequenceable": "1", "405-Name": "DA Shutter", "405 AO-IOChannel": "Dev1/ao0", "Core-Camera": "PRIME", "Focus-Name": "Focus", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "405 Shutter-IOChannel": "Dev1/ao8", "640 AO-Demo": "No", "640 Shutter-SupportsTriggering": "0", "445 AO-Demo": "No", "555-DA Device": "555 Shutter", "470 Shutter-Description": "NI DAC", "528 AO-Volts": "0.0000", "COM1-DataBits": "8", "Vincent-D3-Address": "x", "520 AO-SupportsTriggering": "0", "405-Description": "DA device that is used as a shutter", "640 Shutter-MinVolts": "0.0000", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "405 AO-Name": "AnalogIO", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "640 AO-TriggerInputLine": "/Dev1/PFI0", "405 AO-TriggerInputLine": "/Dev1/PFI0", "640-Description": "DA device that is used as a shutter", "445 Shutter-TriggerSampleRate": "100000", "470 Shutter-TriggerSequenceLength": "1000", "COM1-StopBits": "1", "520 Shutter-SupportsTriggering": "0", "405-State": "1", "Core-Initialize": "1", "XLIGHT Emission Wheel-State": "3", "520 AO-Description": "NI DAC", "528 AO-TriggerInputLine": "/Dev1/PFI0", "445 Shutter-MaxVolts": "5.0000", "COM9-Handshaking": "Off", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "XLIGHT Spin motor-Label": "On (spinning)", "PRIME-PP 5 ENABLED": "No", "UUID": "4c774f64-1938-4e33-be1b-f801c4463959", "PRIME-TriggerMode": "Internal Trigger", "COM1-Handshaking": "Off", "PRIME-Timing-PostTriggerDelayNs": "49344000.0000", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "Focus-Position": "17741.5000", "528 Shutter-Block voltage": "false", "Channel": "P-DAPI-high", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "555 Shutter-Demo": "No", "405 Shutter-Demo": "No", "528 AO-Name": "AnalogIO", "520-State": "0", "Core-Galvo": "", "405 Shutter-TriggerSampleRate": "100000", "Focus-FocusStepSize_(nm)": "100", "PRIME-PixelType": "16bit", "COM4-DataBits": "8", "470 AO-IOChannel": "Dev1/ao2", "520 AO-MinVolts": "0.0000", "COM9-Parity": "None", "PRIME-CircularBufferEnabled": "ON", "PRIME-PP 6 Reset": "No", "640 AO-Description": "NI DAC", "Source": "PRIME", "445 AO-MinVolts": "0.0000", "PIEZO-Name": "Stage", "445 AO-Description": "NI DAC", "PRIME-CircularBufferFrameCount": "46", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "520 AO-Volts": "0.0000", "528 Shutter-TriggerSampleRate": "100000", "XPositionUm": 19731.9, "OpticalPath-State": "4", "PRIME-PP 3 THRESHOLD": "80", "PRIME-PVCAM Version": "3.7.1", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Gain": "1-HDR", "640 Shutter-TriggerSequenceLength": "1000", "528 AO-SupportsTriggering": "0", "445 AO-TriggerInputLine": "/Dev1/PFI0", "OpticalPath-Name": "OpticalPath", "PRIME-PP 4 ENABLED": "Yes", "PRIME-PVCAM Adapter Version": "1.3.29", "COM3-Name": "COM3", "405 AO-MaxVolts": "5.0000", "520 Shutter-Block voltage": "false", "PRIME-Timing-ClearingTimeNs": "49344000.0000", "Core-Focus": "PIEZO", "528 AO-Block voltage": "false", "TE2000-Description": "Nikon TE2000 hub", "528 AO-MinVolts": "0.0000", "COM4-Parity": "None", "Summary": { "Slices": 11, "ChContrastMin": [ 0, 0, 0, 0, 0, 0 ], "PixelAspect": 1, "SlicesFirst": true, "Interval_ms": 0, "Time": "2019-12-18 21:36:42 -0500", "Prefix": "seq_4", "InitialPositionList": [ { "Label": "Pos0", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.21], "Focus": [17741.5], "XYStage": [ 44542.200000000004, 31636.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos1", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.22], "Focus": [17741.4], "XYStage": [ 43801.100000000006, 31637.9 ] }, "GridColumnIndex": 0 }, { "Label": "Pos2", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.5], "XYStage": [ 38561.3, 32040.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos3", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.185], "Focus": [17741.3], "XYStage": [ 37698.4, 32041.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos4", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.16], "Focus": [17741.5], "XYStage": [ 32375.4, 31758.9 ] }, "GridColumnIndex": 0 }, { "Label": "Pos5", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.17000000000002], "Focus": [17741.3], "XYStage": [ 31770.7, 31758.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos6", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.4], "XYStage": [ 26536.800000000003, 31068.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos7", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.205], "Focus": [17741.4], "XYStage": [ 25420.2, 31068.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos8", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.85], "Focus": [17741.3], "XYStage": [ 21059, 31508 ] }, "GridColumnIndex": 0 }, { "Label": "Pos9", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.82], "Focus": [17741.3], "XYStage": [ 19731.9, 31506.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos10", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.79], "Focus": [17741.4], "XYStage": [ 14528.5, 32007.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos11", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.83], "Focus": [17741.2], "XYStage": [ 13830.300000000001, 32009.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos12", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.79], "Focus": [17741.3], "XYStage": [ 8775.300000000001, 32017.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos13", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.815], "Focus": [17741.3], "XYStage": [ 8109.200000000001, 32008.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos14", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.805], "Focus": [17741.3], "XYStage": [ 3483.3, 31908.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos15", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.82], "Focus": [17741.2], "XYStage": [ 2686.8, 31903.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos16", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [211.48000000000002], "Focus": [17741.1], "XYStage": [ -1978.9, 31973.600000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos17", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [211.51500000000001], "Focus": [17741.1], "XYStage": [ -2957.8, 31982.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos18", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.195], "Focus": [17741.5], "XYStage": [ 47747.4, -576.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos19", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.23000000000002], "Focus": [17741.6], "XYStage": [ 47053.9, -576.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos20", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.205], "Focus": [17741.4], "XYStage": [ 41871.200000000004, -479.8 ] }, "GridColumnIndex": 0 }, { "Label": "Pos21", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.4], "XYStage": [ 40882.600000000006, -392 ] }, "GridColumnIndex": 0 }, { "Label": "Pos22", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.24], "Focus": [17741.4], "XYStage": [ 35905.6, -135.3 ] }, "GridColumnIndex": 0 }, { "Label": "Pos23", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.195], "Focus": [17741.5], "XYStage": [ 34990.9, -132.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos24", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.46], "Focus": [17741.4], "XYStage": [ 29678.100000000002, -958.1 ] }, "GridColumnIndex": 0 }, { "Label": "Pos25", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.44], "Focus": [17741.5], "XYStage": [ 28983.600000000002, -939.4000000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos26", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.475], "Focus": [17741.5], "XYStage": [ 23019.7, -122 ] }, "GridColumnIndex": 0 }, { "Label": "Pos27", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.44], "Focus": [17741.7], "XYStage": [ 22428, -116.80000000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos28", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.46], "Focus": [17741.7], "XYStage": [ 16364.900000000001, -181.10000000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos29", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.48000000000002], "Focus": [17741.5], "XYStage": [ 15712.1, -180 ] }, "GridColumnIndex": 0 }, { "Label": "Pos30", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.45000000000002], "Focus": [17741.7], "XYStage": [ 10346.1, 77.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos31", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.49], "Focus": [17741.4], "XYStage": [ 9755.5, 85.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos32", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.475], "Focus": [17741.5], "XYStage": [ 4775.5, 293.90000000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos33", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.445], "Focus": [17741.4], "XYStage": [ 3954.9, 293.90000000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos34", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.455], "Focus": [17741.4], "XYStage": [ -1324.9, 545.6 ] }, "GridColumnIndex": 0 }, { "Label": "Pos35", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.445], "Focus": [17741.3], "XYStage": [ -2097, 576.6 ] }, "GridColumnIndex": 0 } ], "z-step_um": 10, "GridRow": 0, "BitDepth": 16, "Depth": 2, "Channels": 6, "Source": "Micro-Manager", "ChColors": [ -16711681, -256, -65281, -1, -1, -16776961 ], "MetadataVersion": 10, "KeepShutterOpenChannels": true, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536, 65536 ], "CustomIntervals_ms": [], "Height": 1200, "MicroManagerVersion": "1.4.23 20180219", "GridColumn": 0, "UUID": "14a13bf7-c944-4672-b873-fcfbbb7ea13d", "PixelType": "GRAY16", "Width": 1200, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\Yan\\20191218-YC113-S7S8-miseq", "Frames": 1, "Comment": "", "UserName": "Nikon", "PixelSize_um": 1.1, "ROI": [ 0, 0, 1200, 1200 ], "Date": "2019-12-18", "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC", "P-DAPI-high" ], "KeepShutterOpenSlices": true, "IJType": 1, "ComputerName": "ZadorConfocal2", "Positions": 36, "PositionIndex": 9, "CameraTimeout": 20000, "TimeFirst": false }, "640 AO-Name": "AnalogIO", "Vincent-D3-Command": "Close" }, "FrameKey-0-5-2": { "COM4-StopBits": "1", "528 AO-MaxVolts": "5.0000", "XLIGHT Excitation Wheel-State": "1", "470 Shutter-Name": "AnalogIO", "Core-Shutter": "405", "555 AO-Demo": "No", "XLIGHT Dichroic-State": "2", "XYStage-StepSize-Y": "0.1000", "ZPositionUm": 218.82, "XYStage-StepSize-X": "0.1000", "PRIME-FirmwareVersion": "20.28", "PRIME-ClearCycles": "2", "520-Name": "DA Shutter", "405 Shutter-Name": "AnalogIO", "PIEZO-StepSize": "0.0050", "PRIME-ClearMode": "Pre-Exposure", "470 AO-MinVolts": "0.0000", "405 AO-SupportsTriggering": "1", "CameraChannelIndex": 0, "XYStage-StartSpeed": "500.0000", "COM9-StopBits": "2", "405 AO-Description": "NI DAC", "COM4-DelayBetweenCharsMs": "0.0000", "445 AO-Block voltage": "false", "520 AO-TriggerInputLine": "/Dev1/PFI0", "555 AO-Description": "NI DAC", "520 AO-TriggerSampleRate": "100000", "COM4-Verbose": "1", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM4-Name": "COM4", "405 Shutter-Volts": "5.0000", "Vincent-D3-Port": "COM1", "PRIME-PP 4 MIN ADU AFFECTED": "200", "470 Shutter-MinVolts": "0.0000", "PRIME-PP 3 ENABLED": "Yes", "470 AO-Description": "NI DAC", "PRIME-Description": "PVCAM API device adapter", "520 Shutter-MaxVolts": "5.0000", "520 Shutter-Volts": "0.0000", "528 AO-Sequenceable": "1", "PRIME-Binning": "1x1", "555 Shutter-MaxVolts": "5.0000", "528 Shutter-TriggerSequenceLength": "1000", "405 Shutter-Block voltage": "false", "XLIGHT Spin motor-State": "1", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "Vincent-D3-Time to open (ms)": "35.0000", "Vincent-D3-Time to close (ms)": "35.0000", "445 AO-TriggerSampleRate": "100000", "640 Shutter-IOChannel": "Dev1/ao14", "555 AO-Sequenceable": "1", "520 AO-Name": "AnalogIO", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "470 AO-Block voltage": "false", "COM1-Verbose": "1", "COM9-AnswerTimeout": "10000.0000", "528 Shutter-Name": "AnalogIO", "PRIME-Trigger Timeout (secs)": "10", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "NextFrame": 0, "470 AO-TriggerSequenceLength": "1000", "PRIME-TransposeXY": "0", "445 AO-TriggerSequenceLength": "1000", "COM1-Name": "COM1", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "COM9-DataBits": "8", "COM1-AnswerTimeout": "500.0000", "Camera": "", "TE2000-Version": "V2.50-V2.50-V2.10", "528 Shutter-Volts": "0.0000", "XLIGHT Hub-Name": "XLIGHT Hub", "PIEZO-Description": "Ludl stage driver adapter", "PositionName": "Pos9", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSampleRate": "100000", "PRIME-FanSpeedSetpoint": "High", "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "405 AO-TriggerSampleRate": "100000", "470 Shutter-Block voltage": "false", "470-State": "0", "COM3-Parity": "None", "405 AO-Volts": "4.5000", "PRIME-TransposeMirrorY": "0", "PRIME-ChipName": "GS144BSI", "470 AO-Volts": "0.0000", "PRIME-TransposeMirrorX": "0", "445 Shutter-SupportsTriggering": "0", "470 Shutter-SupportsTriggering": "0", "XLIGHT Hub-Description": "XLIGHT hub", "PRIME-PreampOffLimit": "10000", "470 AO-Sequenceable": "1", "528 Shutter-MaxVolts": "5.0000", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "Core-XYStage": "XYStage", "470 AO-TriggerSampleRate": "100000", "PRIME-Timing-PreTriggerDelayNs": "24672000.0000", "528-DA Device": "528 Shutter", "Focus-Description": "Nikon TE2000 focus adapter", "FilterBlock-State": "5", "520 AO-Demo": "No", "COM3-DelayBetweenCharsMs": "0.0000", "470 Shutter-Volts": "0.0000", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "PRIME-PP 2 THRESHOLD": "125", "PRIME-PP 4 THRESHOLD": "75", "XYStage-Acceleration": "4.0000", "470 Shutter-Demo": "No", "PRIME-Name": "PMPCIECam00", "640-DA Device": "640 Shutter", "405 AO-Block voltage": "false", "ElapsedTime-ms": 408091, "PRIME-Offset": "100", "445 AO-IOChannel": "Dev1/ao1", "640 AO-MaxVolts": "5.0000", "PositionIndex": 9, "PRIME-PP 1 ENABLED": "Yes", "COM1-BaudRate": "9600", "640-State": "0", "COM3-Verbose": "1", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "LudlController-TransmissionDelay": "0", "PRIME-FullWellCapacity": "62000", "PRIME-SMARTStreamingEnabled": "No", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "640 Shutter-Description": "NI DAC", "528-Description": "DA device that is used as a shutter", "COM4-Description": "Serial port driver (boost:asio)", "405 Shutter-Description": "NI DAC", "TE2000-Name": "TE2000", "528 Shutter-Description": "NI DAC", "COM9-Description": "Serial port driver (boost:asio)", "LudlController-Name": "LudlController", "445 Shutter-TriggerSequenceLength": "1000", "PRIME-SerialNumber": "A18B203008", "445 AO-Volts": "0.0000", "PRIME-X-dimension": "1200", "PRIME-ShutterCloseDelay": "0", "XLIGHT Disk slider-State": "2", "Position": "Default", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 AO-IOChannel": "Dev1/ao5", "520 AO-IOChannel": "Dev1/ao4", "528 AO-TriggerSequenceLength": "1000", "405 Shutter-MaxVolts": "5.0000", "470-DA Device": "470 Shutter", "PRIME-FTCapable": "No", "Core-ChannelGroup": "Channels", "555 AO-SupportsTriggering": "0", "PRIME-Y-dimension": "1200", "Frame": 0, "555 Shutter-Sequenceable": "1", "PRIME-Timing-ExposureTimeNs": "499998000.0000", "Core-ImageProcessor": "", "528-State": "0", "520 AO-Block voltage": "false", "XLIGHT Excitation Wheel-Label": "QUAD", "640 Shutter-Demo": "No", "COM3-StopBits": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "520 Shutter-IOChannel": "Dev1/ao11", "Width": 1200, "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-SupportsTriggering": "0", "640 AO-TriggerSampleRate": "100000", "520-Description": "DA device that is used as a shutter", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "405 Shutter-Sequenceable": "1", "405 Shutter-SupportsTriggering": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-TriggerSampleRate": "100000", "640 Shutter-MaxVolts": "5.0000", "555 AO-MaxVolts": "5.0000", "520 AO-TriggerSequenceLength": "1000", "COM3-BaudRate": "9600", "COM9-BaudRate": "9600", "Vincent-D3-Channel #": "Ch. #1", "PRIME-PMode": "Normal", "OpticalPath-Label": "Left(100)", "BitDepth": 16, "555 AO-TriggerSequenceLength": "1000", "445 Shutter-Description": "NI DAC", "405 AO-Sequenceable": "1", "PRIME-AcquisitionMethod": "Callbacks", "555 AO-Name": "AnalogIO", "PRIME-ShutterMode": "Pre-Exposure", "COM3-Handshaking": "Off", "445 AO-SupportsTriggering": "0", "ROI": "0-0-1200-1200", "COM9-Verbose": "1", "PRIME-Color": "OFF", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "PRIME-ShutterOpenDelay": "0", "COM1-Parity": "None", "Slice": 2, "SlicePosition": -30, "COM9-DelayBetweenCharsMs": "0.0000", "555-Name": "DA Shutter", "PIEZO-LudlSingleAxisName": "B", "XLIGHT Emission Wheel-Label": "QUAD", "640 Shutter-Name": "AnalogIO", "640 AO-Sequenceable": "1", "520 Shutter-Demo": "No", "528 Shutter-Demo": "No", "470-Name": "DA Shutter", "520-DA Device": "520 Shutter", "XYStage-Description": "Ludl XY stage driver adapter", "445 Shutter-MinVolts": "0.0000", "528 AO-TriggerSampleRate": "100000", "405 AO-MinVolts": "0.0000", "405 Shutter-TriggerSequenceLength": "1000", "Height": 1200, "555 AO-Block voltage": "false", "640 Shutter-Volts": "0.0000", "PixelType": "GRAY16", "445 Shutter-Block voltage": "false", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "520 Shutter-TriggerSequenceLength": "1000", "COM9-Name": "COM9", "Core-SLM": "", "555 AO-Volts": "0.0000", "555 Shutter-Block voltage": "false", "XYStage-Speed": "1800.0000", "520 AO-MaxVolts": "5.0000", "528 Shutter-SupportsTriggering": "0", "COM1-Description": "Serial port driver (boost:asio)", "PRIME-MetadataEnabled": "No", "520 Shutter-MinVolts": "0.0000", "470 Shutter-Sequenceable": "1", "PRIME-PreampDelay": "0", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-MinVolts": "0.0000", "ChannelIndex": 5, "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Core-AutoShutter": "1", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "Exposure-ms": 300, "PRIME-PP 2 ENABLED": "Yes", "640 Shutter-TriggerSampleRate": "100000", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "470 Shutter-IOChannel": "Dev1/ao10", "405 AO-Demo": "No", "PixelSizeUm": 1.1, "470 AO-Demo": "No", "470 Shutter-MaxVolts": "5.0000", "Vincent-D3-Name": "Vincent-D3", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FileName": "img_000000000_P-DAPI-high_002.tif", "LudlController-Reset": "Operate", "PRIME-Exposure": "300.00", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "445-Name": "DA Shutter", "555 Shutter-Name": "AnalogIO", "520 Shutter-TriggerSampleRate": "100000", "445 Shutter-IOChannel": "Dev1/ao9", "PRIME-TransposeCorrection": "0", "XYStage-StepSize": "0.1000", "445-Description": "DA device that is used as a shutter", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 AO-IOChannel": "Dev1/ao7", "520 Shutter-Name": "AnalogIO", "COM3-DataBits": "8", "640 AO-Volts": "0.0000", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "555 Shutter-IOChannel": "Dev1/ao13", "WaitInterval": null, "520 Shutter-Sequenceable": "1", "YPositionUm": 31506.2, "445-State": "0", "445-DA Device": "445 Shutter", "PRIME-CircularBufferAutoSize": "ON", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "555 AO-TriggerSampleRate": "100000", "445 Shutter-Volts": "0.0000", "XLIGHT Hub-Port": "COM3", "Core-AutoFocus": "", "COM4-Handshaking": "Off", "528 Shutter-MinVolts": "0.0000", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "445 Shutter-Name": "AnalogIO", "528 Shutter-Sequenceable": "1", "XYStage-TransposeMirrorX": "0", "XYStage-TransposeMirrorY": "0", "PRIME-ReadoutRate": "100MHz 16bit", "XYStage-Name": "Stage", "640 AO-SupportsTriggering": "0", "640-Name": "DA Shutter", "640 Shutter-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "640 AO-Block voltage": "false", "445 Shutter-Sequenceable": "1", "470 AO-MaxVolts": "5.0000", "COM4-BaudRate": "9600", "470-Description": "DA device that is used as a shutter", "520 Shutter-Description": "NI DAC", "PRIME-CCDTemperature": "-14.8500", "Time": "2019-12-18 21:37:16 -0500", "640 AO-TriggerSequenceLength": "1000", "470 AO-Name": "AnalogIO", "XLIGHT Disk slider-Label": "Disk Pos 40um", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "Binning": "1x1", "TE2000-ModelType": "1", "COM3-AnswerTimeout": "500.0000", "555 Shutter-SupportsTriggering": "0", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "TE2000-Port": "COM4", "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Description": "NI DAC", "COM4-AnswerTimeout": "500.0000", "COM1-DelayBetweenCharsMs": "0.0000", "FilterBlock-Label": "Confocal", "445 AO-Name": "AnalogIO", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-Timing-ReadoutTimeNs": "24672000.0000", "528 AO-Demo": "No", "520 AO-Sequenceable": "1", "555-Description": "DA device that is used as a shutter", "405-DA Device": "405 Shutter", "405 AO-TriggerSequenceLength": "1000", "640 AO-MinVolts": "0.0000", "640 Shutter-Block voltage": "false", "FrameIndex": 0, "PRIME-Port": "CMOS", "555 AO-MinVolts": "0.0000", "COM3-Description": "Serial port driver (boost:asio)", "528 AO-Description": "NI DAC", "555 AO-IOChannel": "Dev1/ao6", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "555 AO-TriggerInputLine": "/Dev1/PFI0", "PRIME-ActualInterval-ms": "390.5200", "LudlController-Port": "COM9", "445 Shutter-Demo": "No", "PRIME-Trigger-Expose Out-Mux": "1", "SliceIndex": 2, "PRIME-CCDTemperatureSetPoint": "-15.0000", "405 Shutter-MinVolts": "0.0000", "PRIME-ExposeOutMode": "First Row", "AxisPositions": null, "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Volts": "0.0000", "528-Name": "DA Shutter", "555-State": "0", "XLIGHT Dichroic-Label": "QUAD", "445 AO-Sequenceable": "1", "405-Name": "DA Shutter", "405 AO-IOChannel": "Dev1/ao0", "Core-Camera": "PRIME", "Focus-Name": "Focus", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "405 Shutter-IOChannel": "Dev1/ao8", "640 AO-Demo": "No", "640 Shutter-SupportsTriggering": "0", "445 AO-Demo": "No", "555-DA Device": "555 Shutter", "470 Shutter-Description": "NI DAC", "528 AO-Volts": "0.0000", "COM1-DataBits": "8", "Vincent-D3-Address": "x", "520 AO-SupportsTriggering": "0", "405-Description": "DA device that is used as a shutter", "640 Shutter-MinVolts": "0.0000", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "405 AO-Name": "AnalogIO", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "640 AO-TriggerInputLine": "/Dev1/PFI0", "405 AO-TriggerInputLine": "/Dev1/PFI0", "640-Description": "DA device that is used as a shutter", "445 Shutter-TriggerSampleRate": "100000", "470 Shutter-TriggerSequenceLength": "1000", "COM1-StopBits": "1", "520 Shutter-SupportsTriggering": "0", "405-State": "1", "Core-Initialize": "1", "XLIGHT Emission Wheel-State": "3", "520 AO-Description": "NI DAC", "528 AO-TriggerInputLine": "/Dev1/PFI0", "445 Shutter-MaxVolts": "5.0000", "COM9-Handshaking": "Off", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "XLIGHT Spin motor-Label": "On (spinning)", "PRIME-PP 5 ENABLED": "No", "UUID": "59380aea-2cb2-4548-b2fc-19c044cd255e", "PRIME-TriggerMode": "Internal Trigger", "COM1-Handshaking": "Off", "PRIME-Timing-PostTriggerDelayNs": "49344000.0000", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "Focus-Position": "17741.5000", "528 Shutter-Block voltage": "false", "Channel": "P-DAPI-high", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "555 Shutter-Demo": "No", "405 Shutter-Demo": "No", "528 AO-Name": "AnalogIO", "520-State": "0", "Core-Galvo": "", "405 Shutter-TriggerSampleRate": "100000", "Focus-FocusStepSize_(nm)": "100", "PRIME-PixelType": "16bit", "COM4-DataBits": "8", "470 AO-IOChannel": "Dev1/ao2", "520 AO-MinVolts": "0.0000", "COM9-Parity": "None", "PRIME-CircularBufferEnabled": "ON", "PRIME-PP 6 Reset": "No", "640 AO-Description": "NI DAC", "Source": "PRIME", "445 AO-MinVolts": "0.0000", "PIEZO-Name": "Stage", "445 AO-Description": "NI DAC", "PRIME-CircularBufferFrameCount": "46", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "520 AO-Volts": "0.0000", "528 Shutter-TriggerSampleRate": "100000", "XPositionUm": 19731.9, "OpticalPath-State": "4", "PRIME-PP 3 THRESHOLD": "80", "PRIME-PVCAM Version": "3.7.1", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Gain": "1-HDR", "640 Shutter-TriggerSequenceLength": "1000", "528 AO-SupportsTriggering": "0", "445 AO-TriggerInputLine": "/Dev1/PFI0", "OpticalPath-Name": "OpticalPath", "PRIME-PP 4 ENABLED": "Yes", "PRIME-PVCAM Adapter Version": "1.3.29", "COM3-Name": "COM3", "405 AO-MaxVolts": "5.0000", "520 Shutter-Block voltage": "false", "PRIME-Timing-ClearingTimeNs": "49344000.0000", "Core-Focus": "PIEZO", "528 AO-Block voltage": "false", "TE2000-Description": "Nikon TE2000 hub", "528 AO-MinVolts": "0.0000", "COM4-Parity": "None", "Summary": { "Slices": 11, "ChContrastMin": [ 0, 0, 0, 0, 0, 0 ], "PixelAspect": 1, "SlicesFirst": true, "Interval_ms": 0, "Time": "2019-12-18 21:36:42 -0500", "Prefix": "seq_4", "InitialPositionList": [ { "Label": "Pos0", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.21], "Focus": [17741.5], "XYStage": [ 44542.200000000004, 31636.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos1", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.22], "Focus": [17741.4], "XYStage": [ 43801.100000000006, 31637.9 ] }, "GridColumnIndex": 0 }, { "Label": "Pos2", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.5], "XYStage": [ 38561.3, 32040.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos3", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.185], "Focus": [17741.3], "XYStage": [ 37698.4, 32041.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos4", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.16], "Focus": [17741.5], "XYStage": [ 32375.4, 31758.9 ] }, "GridColumnIndex": 0 }, { "Label": "Pos5", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.17000000000002], "Focus": [17741.3], "XYStage": [ 31770.7, 31758.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos6", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.4], "XYStage": [ 26536.800000000003, 31068.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos7", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.205], "Focus": [17741.4], "XYStage": [ 25420.2, 31068.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos8", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.85], "Focus": [17741.3], "XYStage": [ 21059, 31508 ] }, "GridColumnIndex": 0 }, { "Label": "Pos9", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.82], "Focus": [17741.3], "XYStage": [ 19731.9, 31506.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos10", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.79], "Focus": [17741.4], "XYStage": [ 14528.5, 32007.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos11", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.83], "Focus": [17741.2], "XYStage": [ 13830.300000000001, 32009.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos12", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.79], "Focus": [17741.3], "XYStage": [ 8775.300000000001, 32017.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos13", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.815], "Focus": [17741.3], "XYStage": [ 8109.200000000001, 32008.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos14", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.805], "Focus": [17741.3], "XYStage": [ 3483.3, 31908.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos15", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.82], "Focus": [17741.2], "XYStage": [ 2686.8, 31903.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos16", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [211.48000000000002], "Focus": [17741.1], "XYStage": [ -1978.9, 31973.600000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos17", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [211.51500000000001], "Focus": [17741.1], "XYStage": [ -2957.8, 31982.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos18", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.195], "Focus": [17741.5], "XYStage": [ 47747.4, -576.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos19", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.23000000000002], "Focus": [17741.6], "XYStage": [ 47053.9, -576.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos20", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.205], "Focus": [17741.4], "XYStage": [ 41871.200000000004, -479.8 ] }, "GridColumnIndex": 0 }, { "Label": "Pos21", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.4], "XYStage": [ 40882.600000000006, -392 ] }, "GridColumnIndex": 0 }, { "Label": "Pos22", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.24], "Focus": [17741.4], "XYStage": [ 35905.6, -135.3 ] }, "GridColumnIndex": 0 }, { "Label": "Pos23", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.195], "Focus": [17741.5], "XYStage": [ 34990.9, -132.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos24", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.46], "Focus": [17741.4], "XYStage": [ 29678.100000000002, -958.1 ] }, "GridColumnIndex": 0 }, { "Label": "Pos25", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.44], "Focus": [17741.5], "XYStage": [ 28983.600000000002, -939.4000000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos26", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.475], "Focus": [17741.5], "XYStage": [ 23019.7, -122 ] }, "GridColumnIndex": 0 }, { "Label": "Pos27", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.44], "Focus": [17741.7], "XYStage": [ 22428, -116.80000000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos28", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.46], "Focus": [17741.7], "XYStage": [ 16364.900000000001, -181.10000000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos29", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.48000000000002], "Focus": [17741.5], "XYStage": [ 15712.1, -180 ] }, "GridColumnIndex": 0 }, { "Label": "Pos30", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.45000000000002], "Focus": [17741.7], "XYStage": [ 10346.1, 77.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos31", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.49], "Focus": [17741.4], "XYStage": [ 9755.5, 85.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos32", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.475], "Focus": [17741.5], "XYStage": [ 4775.5, 293.90000000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos33", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.445], "Focus": [17741.4], "XYStage": [ 3954.9, 293.90000000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos34", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.455], "Focus": [17741.4], "XYStage": [ -1324.9, 545.6 ] }, "GridColumnIndex": 0 }, { "Label": "Pos35", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.445], "Focus": [17741.3], "XYStage": [ -2097, 576.6 ] }, "GridColumnIndex": 0 } ], "z-step_um": 10, "GridRow": 0, "BitDepth": 16, "Depth": 2, "Channels": 6, "Source": "Micro-Manager", "ChColors": [ -16711681, -256, -65281, -1, -1, -16776961 ], "MetadataVersion": 10, "KeepShutterOpenChannels": true, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536, 65536 ], "CustomIntervals_ms": [], "Height": 1200, "MicroManagerVersion": "1.4.23 20180219", "GridColumn": 0, "UUID": "14a13bf7-c944-4672-b873-fcfbbb7ea13d", "PixelType": "GRAY16", "Width": 1200, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\Yan\\20191218-YC113-S7S8-miseq", "Frames": 1, "Comment": "", "UserName": "Nikon", "PixelSize_um": 1.1, "ROI": [ 0, 0, 1200, 1200 ], "Date": "2019-12-18", "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC", "P-DAPI-high" ], "KeepShutterOpenSlices": true, "IJType": 1, "ComputerName": "ZadorConfocal2", "Positions": 36, "PositionIndex": 9, "CameraTimeout": 20000, "TimeFirst": false }, "640 AO-Name": "AnalogIO", "Vincent-D3-Command": "Close" }, "FrameKey-0-5-3": { "COM4-StopBits": "1", "528 AO-MaxVolts": "5.0000", "XLIGHT Excitation Wheel-State": "1", "470 Shutter-Name": "AnalogIO", "Core-Shutter": "405", "555 AO-Demo": "No", "XLIGHT Dichroic-State": "2", "XYStage-StepSize-Y": "0.1000", "ZPositionUm": 228.82, "XYStage-StepSize-X": "0.1000", "PRIME-FirmwareVersion": "20.28", "PRIME-ClearCycles": "2", "520-Name": "DA Shutter", "405 Shutter-Name": "AnalogIO", "PIEZO-StepSize": "0.0050", "PRIME-ClearMode": "Pre-Exposure", "470 AO-MinVolts": "0.0000", "405 AO-SupportsTriggering": "1", "CameraChannelIndex": 0, "XYStage-StartSpeed": "500.0000", "COM9-StopBits": "2", "405 AO-Description": "NI DAC", "COM4-DelayBetweenCharsMs": "0.0000", "445 AO-Block voltage": "false", "520 AO-TriggerInputLine": "/Dev1/PFI0", "555 AO-Description": "NI DAC", "520 AO-TriggerSampleRate": "100000", "COM4-Verbose": "1", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM4-Name": "COM4", "405 Shutter-Volts": "5.0000", "Vincent-D3-Port": "COM1", "PRIME-PP 4 MIN ADU AFFECTED": "200", "470 Shutter-MinVolts": "0.0000", "PRIME-PP 3 ENABLED": "Yes", "470 AO-Description": "NI DAC", "PRIME-Description": "PVCAM API device adapter", "520 Shutter-MaxVolts": "5.0000", "520 Shutter-Volts": "0.0000", "528 AO-Sequenceable": "1", "PRIME-Binning": "1x1", "555 Shutter-MaxVolts": "5.0000", "528 Shutter-TriggerSequenceLength": "1000", "405 Shutter-Block voltage": "false", "XLIGHT Spin motor-State": "1", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "Vincent-D3-Time to open (ms)": "35.0000", "Vincent-D3-Time to close (ms)": "35.0000", "445 AO-TriggerSampleRate": "100000", "640 Shutter-IOChannel": "Dev1/ao14", "555 AO-Sequenceable": "1", "520 AO-Name": "AnalogIO", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "470 AO-Block voltage": "false", "COM1-Verbose": "1", "COM9-AnswerTimeout": "10000.0000", "528 Shutter-Name": "AnalogIO", "PRIME-Trigger Timeout (secs)": "10", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "NextFrame": 0, "470 AO-TriggerSequenceLength": "1000", "PRIME-TransposeXY": "0", "445 AO-TriggerSequenceLength": "1000", "COM1-Name": "COM1", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "COM9-DataBits": "8", "COM1-AnswerTimeout": "500.0000", "Camera": "", "TE2000-Version": "V2.50-V2.50-V2.10", "528 Shutter-Volts": "0.0000", "XLIGHT Hub-Name": "XLIGHT Hub", "PIEZO-Description": "Ludl stage driver adapter", "PositionName": "Pos9", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSampleRate": "100000", "PRIME-FanSpeedSetpoint": "High", "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "405 AO-TriggerSampleRate": "100000", "470 Shutter-Block voltage": "false", "470-State": "0", "COM3-Parity": "None", "405 AO-Volts": "4.5000", "PRIME-TransposeMirrorY": "0", "PRIME-ChipName": "GS144BSI", "470 AO-Volts": "0.0000", "PRIME-TransposeMirrorX": "0", "445 Shutter-SupportsTriggering": "0", "470 Shutter-SupportsTriggering": "0", "XLIGHT Hub-Description": "XLIGHT hub", "PRIME-PreampOffLimit": "10000", "470 AO-Sequenceable": "1", "528 Shutter-MaxVolts": "5.0000", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "Core-XYStage": "XYStage", "470 AO-TriggerSampleRate": "100000", "PRIME-Timing-PreTriggerDelayNs": "24672000.0000", "528-DA Device": "528 Shutter", "Focus-Description": "Nikon TE2000 focus adapter", "FilterBlock-State": "5", "520 AO-Demo": "No", "COM3-DelayBetweenCharsMs": "0.0000", "470 Shutter-Volts": "0.0000", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "PRIME-PP 2 THRESHOLD": "125", "PRIME-PP 4 THRESHOLD": "75", "XYStage-Acceleration": "4.0000", "470 Shutter-Demo": "No", "PRIME-Name": "PMPCIECam00", "640-DA Device": "640 Shutter", "405 AO-Block voltage": "false", "ElapsedTime-ms": 408683, "PRIME-Offset": "100", "445 AO-IOChannel": "Dev1/ao1", "640 AO-MaxVolts": "5.0000", "PositionIndex": 9, "PRIME-PP 1 ENABLED": "Yes", "COM1-BaudRate": "9600", "640-State": "0", "COM3-Verbose": "1", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "LudlController-TransmissionDelay": "0", "PRIME-FullWellCapacity": "62000", "PRIME-SMARTStreamingEnabled": "No", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "640 Shutter-Description": "NI DAC", "528-Description": "DA device that is used as a shutter", "COM4-Description": "Serial port driver (boost:asio)", "405 Shutter-Description": "NI DAC", "TE2000-Name": "TE2000", "528 Shutter-Description": "NI DAC", "COM9-Description": "Serial port driver (boost:asio)", "LudlController-Name": "LudlController", "445 Shutter-TriggerSequenceLength": "1000", "PRIME-SerialNumber": "A18B203008", "445 AO-Volts": "0.0000", "PRIME-X-dimension": "1200", "PRIME-ShutterCloseDelay": "0", "XLIGHT Disk slider-State": "2", "Position": "Default", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 AO-IOChannel": "Dev1/ao5", "520 AO-IOChannel": "Dev1/ao4", "528 AO-TriggerSequenceLength": "1000", "405 Shutter-MaxVolts": "5.0000", "470-DA Device": "470 Shutter", "PRIME-FTCapable": "No", "Core-ChannelGroup": "Channels", "555 AO-SupportsTriggering": "0", "PRIME-Y-dimension": "1200", "Frame": 0, "555 Shutter-Sequenceable": "1", "PRIME-Timing-ExposureTimeNs": "499998000.0000", "Core-ImageProcessor": "", "528-State": "0", "520 AO-Block voltage": "false", "XLIGHT Excitation Wheel-Label": "QUAD", "640 Shutter-Demo": "No", "COM3-StopBits": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "520 Shutter-IOChannel": "Dev1/ao11", "Width": 1200, "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-SupportsTriggering": "0", "640 AO-TriggerSampleRate": "100000", "520-Description": "DA device that is used as a shutter", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "405 Shutter-Sequenceable": "1", "405 Shutter-SupportsTriggering": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-TriggerSampleRate": "100000", "640 Shutter-MaxVolts": "5.0000", "555 AO-MaxVolts": "5.0000", "520 AO-TriggerSequenceLength": "1000", "COM3-BaudRate": "9600", "COM9-BaudRate": "9600", "Vincent-D3-Channel #": "Ch. #1", "PRIME-PMode": "Normal", "OpticalPath-Label": "Left(100)", "BitDepth": 16, "555 AO-TriggerSequenceLength": "1000", "445 Shutter-Description": "NI DAC", "405 AO-Sequenceable": "1", "PRIME-AcquisitionMethod": "Callbacks", "555 AO-Name": "AnalogIO", "PRIME-ShutterMode": "Pre-Exposure", "COM3-Handshaking": "Off", "445 AO-SupportsTriggering": "0", "ROI": "0-0-1200-1200", "COM9-Verbose": "1", "PRIME-Color": "OFF", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "PRIME-ShutterOpenDelay": "0", "COM1-Parity": "None", "Slice": 3, "SlicePosition": -20, "COM9-DelayBetweenCharsMs": "0.0000", "555-Name": "DA Shutter", "PIEZO-LudlSingleAxisName": "B", "XLIGHT Emission Wheel-Label": "QUAD", "640 Shutter-Name": "AnalogIO", "640 AO-Sequenceable": "1", "520 Shutter-Demo": "No", "528 Shutter-Demo": "No", "470-Name": "DA Shutter", "520-DA Device": "520 Shutter", "XYStage-Description": "Ludl XY stage driver adapter", "445 Shutter-MinVolts": "0.0000", "528 AO-TriggerSampleRate": "100000", "405 AO-MinVolts": "0.0000", "405 Shutter-TriggerSequenceLength": "1000", "Height": 1200, "555 AO-Block voltage": "false", "640 Shutter-Volts": "0.0000", "PixelType": "GRAY16", "445 Shutter-Block voltage": "false", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "520 Shutter-TriggerSequenceLength": "1000", "COM9-Name": "COM9", "Core-SLM": "", "555 AO-Volts": "0.0000", "555 Shutter-Block voltage": "false", "XYStage-Speed": "1800.0000", "520 AO-MaxVolts": "5.0000", "528 Shutter-SupportsTriggering": "0", "COM1-Description": "Serial port driver (boost:asio)", "PRIME-MetadataEnabled": "No", "520 Shutter-MinVolts": "0.0000", "470 Shutter-Sequenceable": "1", "PRIME-PreampDelay": "0", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-MinVolts": "0.0000", "ChannelIndex": 5, "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Core-AutoShutter": "1", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "Exposure-ms": 300, "PRIME-PP 2 ENABLED": "Yes", "640 Shutter-TriggerSampleRate": "100000", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "470 Shutter-IOChannel": "Dev1/ao10", "405 AO-Demo": "No", "PixelSizeUm": 1.1, "470 AO-Demo": "No", "470 Shutter-MaxVolts": "5.0000", "Vincent-D3-Name": "Vincent-D3", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FileName": "img_000000000_P-DAPI-high_003.tif", "LudlController-Reset": "Operate", "PRIME-Exposure": "300.00", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "445-Name": "DA Shutter", "555 Shutter-Name": "AnalogIO", "520 Shutter-TriggerSampleRate": "100000", "445 Shutter-IOChannel": "Dev1/ao9", "PRIME-TransposeCorrection": "0", "XYStage-StepSize": "0.1000", "445-Description": "DA device that is used as a shutter", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 AO-IOChannel": "Dev1/ao7", "520 Shutter-Name": "AnalogIO", "COM3-DataBits": "8", "640 AO-Volts": "0.0000", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "555 Shutter-IOChannel": "Dev1/ao13", "WaitInterval": null, "520 Shutter-Sequenceable": "1", "YPositionUm": 31506.2, "445-State": "0", "445-DA Device": "445 Shutter", "PRIME-CircularBufferAutoSize": "ON", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "555 AO-TriggerSampleRate": "100000", "445 Shutter-Volts": "0.0000", "XLIGHT Hub-Port": "COM3", "Core-AutoFocus": "", "COM4-Handshaking": "Off", "528 Shutter-MinVolts": "0.0000", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "445 Shutter-Name": "AnalogIO", "528 Shutter-Sequenceable": "1", "XYStage-TransposeMirrorX": "0", "XYStage-TransposeMirrorY": "0", "PRIME-ReadoutRate": "100MHz 16bit", "XYStage-Name": "Stage", "640 AO-SupportsTriggering": "0", "640-Name": "DA Shutter", "640 Shutter-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "640 AO-Block voltage": "false", "445 Shutter-Sequenceable": "1", "470 AO-MaxVolts": "5.0000", "COM4-BaudRate": "9600", "470-Description": "DA device that is used as a shutter", "520 Shutter-Description": "NI DAC", "PRIME-CCDTemperature": "-14.8500", "Time": "2019-12-18 21:37:16 -0500", "640 AO-TriggerSequenceLength": "1000", "470 AO-Name": "AnalogIO", "XLIGHT Disk slider-Label": "Disk Pos 40um", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "Binning": "1x1", "TE2000-ModelType": "1", "COM3-AnswerTimeout": "500.0000", "555 Shutter-SupportsTriggering": "0", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "TE2000-Port": "COM4", "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Description": "NI DAC", "COM4-AnswerTimeout": "500.0000", "COM1-DelayBetweenCharsMs": "0.0000", "FilterBlock-Label": "Confocal", "445 AO-Name": "AnalogIO", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-Timing-ReadoutTimeNs": "24672000.0000", "528 AO-Demo": "No", "520 AO-Sequenceable": "1", "555-Description": "DA device that is used as a shutter", "405-DA Device": "405 Shutter", "405 AO-TriggerSequenceLength": "1000", "640 AO-MinVolts": "0.0000", "640 Shutter-Block voltage": "false", "FrameIndex": 0, "PRIME-Port": "CMOS", "555 AO-MinVolts": "0.0000", "COM3-Description": "Serial port driver (boost:asio)", "528 AO-Description": "NI DAC", "555 AO-IOChannel": "Dev1/ao6", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "555 AO-TriggerInputLine": "/Dev1/PFI0", "PRIME-ActualInterval-ms": "390.5200", "LudlController-Port": "COM9", "445 Shutter-Demo": "No", "PRIME-Trigger-Expose Out-Mux": "1", "SliceIndex": 3, "PRIME-CCDTemperatureSetPoint": "-15.0000", "405 Shutter-MinVolts": "0.0000", "PRIME-ExposeOutMode": "First Row", "AxisPositions": null, "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Volts": "0.0000", "528-Name": "DA Shutter", "555-State": "0", "XLIGHT Dichroic-Label": "QUAD", "445 AO-Sequenceable": "1", "405-Name": "DA Shutter", "405 AO-IOChannel": "Dev1/ao0", "Core-Camera": "PRIME", "Focus-Name": "Focus", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "405 Shutter-IOChannel": "Dev1/ao8", "640 AO-Demo": "No", "640 Shutter-SupportsTriggering": "0", "445 AO-Demo": "No", "555-DA Device": "555 Shutter", "470 Shutter-Description": "NI DAC", "528 AO-Volts": "0.0000", "COM1-DataBits": "8", "Vincent-D3-Address": "x", "520 AO-SupportsTriggering": "0", "405-Description": "DA device that is used as a shutter", "640 Shutter-MinVolts": "0.0000", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "405 AO-Name": "AnalogIO", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "640 AO-TriggerInputLine": "/Dev1/PFI0", "405 AO-TriggerInputLine": "/Dev1/PFI0", "640-Description": "DA device that is used as a shutter", "445 Shutter-TriggerSampleRate": "100000", "470 Shutter-TriggerSequenceLength": "1000", "COM1-StopBits": "1", "520 Shutter-SupportsTriggering": "0", "405-State": "1", "Core-Initialize": "1", "XLIGHT Emission Wheel-State": "3", "520 AO-Description": "NI DAC", "528 AO-TriggerInputLine": "/Dev1/PFI0", "445 Shutter-MaxVolts": "5.0000", "COM9-Handshaking": "Off", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "XLIGHT Spin motor-Label": "On (spinning)", "PRIME-PP 5 ENABLED": "No", "UUID": "d25ea482-7e19-471c-8063-02acae054a77", "PRIME-TriggerMode": "Internal Trigger", "COM1-Handshaking": "Off", "PRIME-Timing-PostTriggerDelayNs": "49344000.0000", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "Focus-Position": "17741.5000", "528 Shutter-Block voltage": "false", "Channel": "P-DAPI-high", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "555 Shutter-Demo": "No", "405 Shutter-Demo": "No", "528 AO-Name": "AnalogIO", "520-State": "0", "Core-Galvo": "", "405 Shutter-TriggerSampleRate": "100000", "Focus-FocusStepSize_(nm)": "100", "PRIME-PixelType": "16bit", "COM4-DataBits": "8", "470 AO-IOChannel": "Dev1/ao2", "520 AO-MinVolts": "0.0000", "COM9-Parity": "None", "PRIME-CircularBufferEnabled": "ON", "PRIME-PP 6 Reset": "No", "640 AO-Description": "NI DAC", "Source": "PRIME", "445 AO-MinVolts": "0.0000", "PIEZO-Name": "Stage", "445 AO-Description": "NI DAC", "PRIME-CircularBufferFrameCount": "46", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "520 AO-Volts": "0.0000", "528 Shutter-TriggerSampleRate": "100000", "XPositionUm": 19731.9, "OpticalPath-State": "4", "PRIME-PP 3 THRESHOLD": "80", "PRIME-PVCAM Version": "3.7.1", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Gain": "1-HDR", "640 Shutter-TriggerSequenceLength": "1000", "528 AO-SupportsTriggering": "0", "445 AO-TriggerInputLine": "/Dev1/PFI0", "OpticalPath-Name": "OpticalPath", "PRIME-PP 4 ENABLED": "Yes", "PRIME-PVCAM Adapter Version": "1.3.29", "COM3-Name": "COM3", "405 AO-MaxVolts": "5.0000", "520 Shutter-Block voltage": "false", "PRIME-Timing-ClearingTimeNs": "49344000.0000", "Core-Focus": "PIEZO", "528 AO-Block voltage": "false", "TE2000-Description": "Nikon TE2000 hub", "528 AO-MinVolts": "0.0000", "COM4-Parity": "None", "Summary": { "Slices": 11, "ChContrastMin": [ 0, 0, 0, 0, 0, 0 ], "PixelAspect": 1, "SlicesFirst": true, "Interval_ms": 0, "Time": "2019-12-18 21:36:42 -0500", "Prefix": "seq_4", "InitialPositionList": [ { "Label": "Pos0", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.21], "Focus": [17741.5], "XYStage": [ 44542.200000000004, 31636.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos1", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.22], "Focus": [17741.4], "XYStage": [ 43801.100000000006, 31637.9 ] }, "GridColumnIndex": 0 }, { "Label": "Pos2", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.5], "XYStage": [ 38561.3, 32040.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos3", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.185], "Focus": [17741.3], "XYStage": [ 37698.4, 32041.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos4", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.16], "Focus": [17741.5], "XYStage": [ 32375.4, 31758.9 ] }, "GridColumnIndex": 0 }, { "Label": "Pos5", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.17000000000002], "Focus": [17741.3], "XYStage": [ 31770.7, 31758.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos6", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.4], "XYStage": [ 26536.800000000003, 31068.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos7", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.205], "Focus": [17741.4], "XYStage": [ 25420.2, 31068.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos8", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.85], "Focus": [17741.3], "XYStage": [ 21059, 31508 ] }, "GridColumnIndex": 0 }, { "Label": "Pos9", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.82], "Focus": [17741.3], "XYStage": [ 19731.9, 31506.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos10", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.79], "Focus": [17741.4], "XYStage": [ 14528.5, 32007.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos11", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.83], "Focus": [17741.2], "XYStage": [ 13830.300000000001, 32009.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos12", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.79], "Focus": [17741.3], "XYStage": [ 8775.300000000001, 32017.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos13", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.815], "Focus": [17741.3], "XYStage": [ 8109.200000000001, 32008.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos14", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.805], "Focus": [17741.3], "XYStage": [ 3483.3, 31908.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos15", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.82], "Focus": [17741.2], "XYStage": [ 2686.8, 31903.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos16", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [211.48000000000002], "Focus": [17741.1], "XYStage": [ -1978.9, 31973.600000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos17", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [211.51500000000001], "Focus": [17741.1], "XYStage": [ -2957.8, 31982.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos18", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.195], "Focus": [17741.5], "XYStage": [ 47747.4, -576.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos19", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.23000000000002], "Focus": [17741.6], "XYStage": [ 47053.9, -576.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos20", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.205], "Focus": [17741.4], "XYStage": [ 41871.200000000004, -479.8 ] }, "GridColumnIndex": 0 }, { "Label": "Pos21", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.4], "XYStage": [ 40882.600000000006, -392 ] }, "GridColumnIndex": 0 }, { "Label": "Pos22", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.24], "Focus": [17741.4], "XYStage": [ 35905.6, -135.3 ] }, "GridColumnIndex": 0 }, { "Label": "Pos23", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.195], "Focus": [17741.5], "XYStage": [ 34990.9, -132.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos24", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.46], "Focus": [17741.4], "XYStage": [ 29678.100000000002, -958.1 ] }, "GridColumnIndex": 0 }, { "Label": "Pos25", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.44], "Focus": [17741.5], "XYStage": [ 28983.600000000002, -939.4000000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos26", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.475], "Focus": [17741.5], "XYStage": [ 23019.7, -122 ] }, "GridColumnIndex": 0 }, { "Label": "Pos27", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.44], "Focus": [17741.7], "XYStage": [ 22428, -116.80000000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos28", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.46], "Focus": [17741.7], "XYStage": [ 16364.900000000001, -181.10000000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos29", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.48000000000002], "Focus": [17741.5], "XYStage": [ 15712.1, -180 ] }, "GridColumnIndex": 0 }, { "Label": "Pos30", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.45000000000002], "Focus": [17741.7], "XYStage": [ 10346.1, 77.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos31", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.49], "Focus": [17741.4], "XYStage": [ 9755.5, 85.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos32", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.475], "Focus": [17741.5], "XYStage": [ 4775.5, 293.90000000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos33", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.445], "Focus": [17741.4], "XYStage": [ 3954.9, 293.90000000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos34", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.455], "Focus": [17741.4], "XYStage": [ -1324.9, 545.6 ] }, "GridColumnIndex": 0 }, { "Label": "Pos35", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.445], "Focus": [17741.3], "XYStage": [ -2097, 576.6 ] }, "GridColumnIndex": 0 } ], "z-step_um": 10, "GridRow": 0, "BitDepth": 16, "Depth": 2, "Channels": 6, "Source": "Micro-Manager", "ChColors": [ -16711681, -256, -65281, -1, -1, -16776961 ], "MetadataVersion": 10, "KeepShutterOpenChannels": true, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536, 65536 ], "CustomIntervals_ms": [], "Height": 1200, "MicroManagerVersion": "1.4.23 20180219", "GridColumn": 0, "UUID": "14a13bf7-c944-4672-b873-fcfbbb7ea13d", "PixelType": "GRAY16", "Width": 1200, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\Yan\\20191218-YC113-S7S8-miseq", "Frames": 1, "Comment": "", "UserName": "Nikon", "PixelSize_um": 1.1, "ROI": [ 0, 0, 1200, 1200 ], "Date": "2019-12-18", "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC", "P-DAPI-high" ], "KeepShutterOpenSlices": true, "IJType": 1, "ComputerName": "ZadorConfocal2", "Positions": 36, "PositionIndex": 9, "CameraTimeout": 20000, "TimeFirst": false }, "640 AO-Name": "AnalogIO", "Vincent-D3-Command": "Close" }, "FrameKey-0-5-4": { "COM4-StopBits": "1", "528 AO-MaxVolts": "5.0000", "XLIGHT Excitation Wheel-State": "1", "470 Shutter-Name": "AnalogIO", "Core-Shutter": "405", "555 AO-Demo": "No", "XLIGHT Dichroic-State": "2", "XYStage-StepSize-Y": "0.1000", "ZPositionUm": 238.82, "XYStage-StepSize-X": "0.1000", "PRIME-FirmwareVersion": "20.28", "PRIME-ClearCycles": "2", "520-Name": "DA Shutter", "405 Shutter-Name": "AnalogIO", "PIEZO-StepSize": "0.0050", "PRIME-ClearMode": "Pre-Exposure", "470 AO-MinVolts": "0.0000", "405 AO-SupportsTriggering": "1", "CameraChannelIndex": 0, "XYStage-StartSpeed": "500.0000", "COM9-StopBits": "2", "405 AO-Description": "NI DAC", "COM4-DelayBetweenCharsMs": "0.0000", "445 AO-Block voltage": "false", "520 AO-TriggerInputLine": "/Dev1/PFI0", "555 AO-Description": "NI DAC", "520 AO-TriggerSampleRate": "100000", "COM4-Verbose": "1", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM4-Name": "COM4", "405 Shutter-Volts": "5.0000", "Vincent-D3-Port": "COM1", "PRIME-PP 4 MIN ADU AFFECTED": "200", "470 Shutter-MinVolts": "0.0000", "PRIME-PP 3 ENABLED": "Yes", "470 AO-Description": "NI DAC", "PRIME-Description": "PVCAM API device adapter", "520 Shutter-MaxVolts": "5.0000", "520 Shutter-Volts": "0.0000", "528 AO-Sequenceable": "1", "PRIME-Binning": "1x1", "555 Shutter-MaxVolts": "5.0000", "528 Shutter-TriggerSequenceLength": "1000", "405 Shutter-Block voltage": "false", "XLIGHT Spin motor-State": "1", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "Vincent-D3-Time to open (ms)": "35.0000", "Vincent-D3-Time to close (ms)": "35.0000", "445 AO-TriggerSampleRate": "100000", "640 Shutter-IOChannel": "Dev1/ao14", "555 AO-Sequenceable": "1", "520 AO-Name": "AnalogIO", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "470 AO-Block voltage": "false", "COM1-Verbose": "1", "COM9-AnswerTimeout": "10000.0000", "528 Shutter-Name": "AnalogIO", "PRIME-Trigger Timeout (secs)": "10", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "NextFrame": 0, "470 AO-TriggerSequenceLength": "1000", "PRIME-TransposeXY": "0", "445 AO-TriggerSequenceLength": "1000", "COM1-Name": "COM1", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "COM9-DataBits": "8", "COM1-AnswerTimeout": "500.0000", "Camera": "", "TE2000-Version": "V2.50-V2.50-V2.10", "528 Shutter-Volts": "0.0000", "XLIGHT Hub-Name": "XLIGHT Hub", "PIEZO-Description": "Ludl stage driver adapter", "PositionName": "Pos9", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSampleRate": "100000", "PRIME-FanSpeedSetpoint": "High", "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "405 AO-TriggerSampleRate": "100000", "470 Shutter-Block voltage": "false", "470-State": "0", "COM3-Parity": "None", "405 AO-Volts": "4.5000", "PRIME-TransposeMirrorY": "0", "PRIME-ChipName": "GS144BSI", "470 AO-Volts": "0.0000", "PRIME-TransposeMirrorX": "0", "445 Shutter-SupportsTriggering": "0", "470 Shutter-SupportsTriggering": "0", "XLIGHT Hub-Description": "XLIGHT hub", "PRIME-PreampOffLimit": "10000", "470 AO-Sequenceable": "1", "528 Shutter-MaxVolts": "5.0000", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "Core-XYStage": "XYStage", "470 AO-TriggerSampleRate": "100000", "PRIME-Timing-PreTriggerDelayNs": "24672000.0000", "528-DA Device": "528 Shutter", "Focus-Description": "Nikon TE2000 focus adapter", "FilterBlock-State": "5", "520 AO-Demo": "No", "COM3-DelayBetweenCharsMs": "0.0000", "470 Shutter-Volts": "0.0000", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "PRIME-PP 2 THRESHOLD": "125", "PRIME-PP 4 THRESHOLD": "75", "XYStage-Acceleration": "4.0000", "470 Shutter-Demo": "No", "PRIME-Name": "PMPCIECam00", "640-DA Device": "640 Shutter", "405 AO-Block voltage": "false", "ElapsedTime-ms": 409280, "PRIME-Offset": "100", "445 AO-IOChannel": "Dev1/ao1", "640 AO-MaxVolts": "5.0000", "PositionIndex": 9, "PRIME-PP 1 ENABLED": "Yes", "COM1-BaudRate": "9600", "640-State": "0", "COM3-Verbose": "1", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "LudlController-TransmissionDelay": "0", "PRIME-FullWellCapacity": "62000", "PRIME-SMARTStreamingEnabled": "No", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "640 Shutter-Description": "NI DAC", "528-Description": "DA device that is used as a shutter", "COM4-Description": "Serial port driver (boost:asio)", "405 Shutter-Description": "NI DAC", "TE2000-Name": "TE2000", "528 Shutter-Description": "NI DAC", "COM9-Description": "Serial port driver (boost:asio)", "LudlController-Name": "LudlController", "445 Shutter-TriggerSequenceLength": "1000", "PRIME-SerialNumber": "A18B203008", "445 AO-Volts": "0.0000", "PRIME-X-dimension": "1200", "PRIME-ShutterCloseDelay": "0", "XLIGHT Disk slider-State": "2", "Position": "Default", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 AO-IOChannel": "Dev1/ao5", "520 AO-IOChannel": "Dev1/ao4", "528 AO-TriggerSequenceLength": "1000", "405 Shutter-MaxVolts": "5.0000", "470-DA Device": "470 Shutter", "PRIME-FTCapable": "No", "Core-ChannelGroup": "Channels", "555 AO-SupportsTriggering": "0", "PRIME-Y-dimension": "1200", "Frame": 0, "555 Shutter-Sequenceable": "1", "PRIME-Timing-ExposureTimeNs": "499998000.0000", "Core-ImageProcessor": "", "528-State": "0", "520 AO-Block voltage": "false", "XLIGHT Excitation Wheel-Label": "QUAD", "640 Shutter-Demo": "No", "COM3-StopBits": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "520 Shutter-IOChannel": "Dev1/ao11", "Width": 1200, "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-SupportsTriggering": "0", "640 AO-TriggerSampleRate": "100000", "520-Description": "DA device that is used as a shutter", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "405 Shutter-Sequenceable": "1", "405 Shutter-SupportsTriggering": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-TriggerSampleRate": "100000", "640 Shutter-MaxVolts": "5.0000", "555 AO-MaxVolts": "5.0000", "520 AO-TriggerSequenceLength": "1000", "COM3-BaudRate": "9600", "COM9-BaudRate": "9600", "Vincent-D3-Channel #": "Ch. #1", "PRIME-PMode": "Normal", "OpticalPath-Label": "Left(100)", "BitDepth": 16, "555 AO-TriggerSequenceLength": "1000", "445 Shutter-Description": "NI DAC", "405 AO-Sequenceable": "1", "PRIME-AcquisitionMethod": "Callbacks", "555 AO-Name": "AnalogIO", "PRIME-ShutterMode": "Pre-Exposure", "COM3-Handshaking": "Off", "445 AO-SupportsTriggering": "0", "ROI": "0-0-1200-1200", "COM9-Verbose": "1", "PRIME-Color": "OFF", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "PRIME-ShutterOpenDelay": "0", "COM1-Parity": "None", "Slice": 4, "SlicePosition": -10, "COM9-DelayBetweenCharsMs": "0.0000", "555-Name": "DA Shutter", "PIEZO-LudlSingleAxisName": "B", "XLIGHT Emission Wheel-Label": "QUAD", "640 Shutter-Name": "AnalogIO", "640 AO-Sequenceable": "1", "520 Shutter-Demo": "No", "528 Shutter-Demo": "No", "470-Name": "DA Shutter", "520-DA Device": "520 Shutter", "XYStage-Description": "Ludl XY stage driver adapter", "445 Shutter-MinVolts": "0.0000", "528 AO-TriggerSampleRate": "100000", "405 AO-MinVolts": "0.0000", "405 Shutter-TriggerSequenceLength": "1000", "Height": 1200, "555 AO-Block voltage": "false", "640 Shutter-Volts": "0.0000", "PixelType": "GRAY16", "445 Shutter-Block voltage": "false", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "520 Shutter-TriggerSequenceLength": "1000", "COM9-Name": "COM9", "Core-SLM": "", "555 AO-Volts": "0.0000", "555 Shutter-Block voltage": "false", "XYStage-Speed": "1800.0000", "520 AO-MaxVolts": "5.0000", "528 Shutter-SupportsTriggering": "0", "COM1-Description": "Serial port driver (boost:asio)", "PRIME-MetadataEnabled": "No", "520 Shutter-MinVolts": "0.0000", "470 Shutter-Sequenceable": "1", "PRIME-PreampDelay": "0", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-MinVolts": "0.0000", "ChannelIndex": 5, "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Core-AutoShutter": "1", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "Exposure-ms": 300, "PRIME-PP 2 ENABLED": "Yes", "640 Shutter-TriggerSampleRate": "100000", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "470 Shutter-IOChannel": "Dev1/ao10", "405 AO-Demo": "No", "PixelSizeUm": 1.1, "470 AO-Demo": "No", "470 Shutter-MaxVolts": "5.0000", "Vincent-D3-Name": "Vincent-D3", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FileName": "img_000000000_P-DAPI-high_004.tif", "LudlController-Reset": "Operate", "PRIME-Exposure": "300.00", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "445-Name": "DA Shutter", "555 Shutter-Name": "AnalogIO", "520 Shutter-TriggerSampleRate": "100000", "445 Shutter-IOChannel": "Dev1/ao9", "PRIME-TransposeCorrection": "0", "XYStage-StepSize": "0.1000", "445-Description": "DA device that is used as a shutter", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 AO-IOChannel": "Dev1/ao7", "520 Shutter-Name": "AnalogIO", "COM3-DataBits": "8", "640 AO-Volts": "0.0000", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "555 Shutter-IOChannel": "Dev1/ao13", "WaitInterval": null, "520 Shutter-Sequenceable": "1", "YPositionUm": 31506.2, "445-State": "0", "445-DA Device": "445 Shutter", "PRIME-CircularBufferAutoSize": "ON", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "555 AO-TriggerSampleRate": "100000", "445 Shutter-Volts": "0.0000", "XLIGHT Hub-Port": "COM3", "Core-AutoFocus": "", "COM4-Handshaking": "Off", "528 Shutter-MinVolts": "0.0000", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "445 Shutter-Name": "AnalogIO", "528 Shutter-Sequenceable": "1", "XYStage-TransposeMirrorX": "0", "XYStage-TransposeMirrorY": "0", "PRIME-ReadoutRate": "100MHz 16bit", "XYStage-Name": "Stage", "640 AO-SupportsTriggering": "0", "640-Name": "DA Shutter", "640 Shutter-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "640 AO-Block voltage": "false", "445 Shutter-Sequenceable": "1", "470 AO-MaxVolts": "5.0000", "COM4-BaudRate": "9600", "470-Description": "DA device that is used as a shutter", "520 Shutter-Description": "NI DAC", "PRIME-CCDTemperature": "-14.8500", "Time": "2019-12-18 21:37:17 -0500", "640 AO-TriggerSequenceLength": "1000", "470 AO-Name": "AnalogIO", "XLIGHT Disk slider-Label": "Disk Pos 40um", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "Binning": "1x1", "TE2000-ModelType": "1", "COM3-AnswerTimeout": "500.0000", "555 Shutter-SupportsTriggering": "0", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "TE2000-Port": "COM4", "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Description": "NI DAC", "COM4-AnswerTimeout": "500.0000", "COM1-DelayBetweenCharsMs": "0.0000", "FilterBlock-Label": "Confocal", "445 AO-Name": "AnalogIO", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-Timing-ReadoutTimeNs": "24672000.0000", "528 AO-Demo": "No", "520 AO-Sequenceable": "1", "555-Description": "DA device that is used as a shutter", "405-DA Device": "405 Shutter", "405 AO-TriggerSequenceLength": "1000", "640 AO-MinVolts": "0.0000", "640 Shutter-Block voltage": "false", "FrameIndex": 0, "PRIME-Port": "CMOS", "555 AO-MinVolts": "0.0000", "COM3-Description": "Serial port driver (boost:asio)", "528 AO-Description": "NI DAC", "555 AO-IOChannel": "Dev1/ao6", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "555 AO-TriggerInputLine": "/Dev1/PFI0", "PRIME-ActualInterval-ms": "390.5200", "LudlController-Port": "COM9", "445 Shutter-Demo": "No", "PRIME-Trigger-Expose Out-Mux": "1", "SliceIndex": 4, "PRIME-CCDTemperatureSetPoint": "-15.0000", "405 Shutter-MinVolts": "0.0000", "PRIME-ExposeOutMode": "First Row", "AxisPositions": null, "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Volts": "0.0000", "528-Name": "DA Shutter", "555-State": "0", "XLIGHT Dichroic-Label": "QUAD", "445 AO-Sequenceable": "1", "405-Name": "DA Shutter", "405 AO-IOChannel": "Dev1/ao0", "Core-Camera": "PRIME", "Focus-Name": "Focus", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "405 Shutter-IOChannel": "Dev1/ao8", "640 AO-Demo": "No", "640 Shutter-SupportsTriggering": "0", "445 AO-Demo": "No", "555-DA Device": "555 Shutter", "470 Shutter-Description": "NI DAC", "528 AO-Volts": "0.0000", "COM1-DataBits": "8", "Vincent-D3-Address": "x", "520 AO-SupportsTriggering": "0", "405-Description": "DA device that is used as a shutter", "640 Shutter-MinVolts": "0.0000", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "405 AO-Name": "AnalogIO", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "640 AO-TriggerInputLine": "/Dev1/PFI0", "405 AO-TriggerInputLine": "/Dev1/PFI0", "640-Description": "DA device that is used as a shutter", "445 Shutter-TriggerSampleRate": "100000", "470 Shutter-TriggerSequenceLength": "1000", "COM1-StopBits": "1", "520 Shutter-SupportsTriggering": "0", "405-State": "1", "Core-Initialize": "1", "XLIGHT Emission Wheel-State": "3", "520 AO-Description": "NI DAC", "528 AO-TriggerInputLine": "/Dev1/PFI0", "445 Shutter-MaxVolts": "5.0000", "COM9-Handshaking": "Off", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "XLIGHT Spin motor-Label": "On (spinning)", "PRIME-PP 5 ENABLED": "No", "UUID": "587e43e5-518e-43ca-90d0-8af41aa6183e", "PRIME-TriggerMode": "Internal Trigger", "COM1-Handshaking": "Off", "PRIME-Timing-PostTriggerDelayNs": "49344000.0000", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "Focus-Position": "17741.5000", "528 Shutter-Block voltage": "false", "Channel": "P-DAPI-high", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "555 Shutter-Demo": "No", "405 Shutter-Demo": "No", "528 AO-Name": "AnalogIO", "520-State": "0", "Core-Galvo": "", "405 Shutter-TriggerSampleRate": "100000", "Focus-FocusStepSize_(nm)": "100", "PRIME-PixelType": "16bit", "COM4-DataBits": "8", "470 AO-IOChannel": "Dev1/ao2", "520 AO-MinVolts": "0.0000", "COM9-Parity": "None", "PRIME-CircularBufferEnabled": "ON", "PRIME-PP 6 Reset": "No", "640 AO-Description": "NI DAC", "Source": "PRIME", "445 AO-MinVolts": "0.0000", "PIEZO-Name": "Stage", "445 AO-Description": "NI DAC", "PRIME-CircularBufferFrameCount": "46", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "520 AO-Volts": "0.0000", "528 Shutter-TriggerSampleRate": "100000", "XPositionUm": 19731.9, "OpticalPath-State": "4", "PRIME-PP 3 THRESHOLD": "80", "PRIME-PVCAM Version": "3.7.1", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Gain": "1-HDR", "640 Shutter-TriggerSequenceLength": "1000", "528 AO-SupportsTriggering": "0", "445 AO-TriggerInputLine": "/Dev1/PFI0", "OpticalPath-Name": "OpticalPath", "PRIME-PP 4 ENABLED": "Yes", "PRIME-PVCAM Adapter Version": "1.3.29", "COM3-Name": "COM3", "405 AO-MaxVolts": "5.0000", "520 Shutter-Block voltage": "false", "PRIME-Timing-ClearingTimeNs": "49344000.0000", "Core-Focus": "PIEZO", "528 AO-Block voltage": "false", "TE2000-Description": "Nikon TE2000 hub", "528 AO-MinVolts": "0.0000", "COM4-Parity": "None", "Summary": { "Slices": 11, "ChContrastMin": [ 0, 0, 0, 0, 0, 0 ], "PixelAspect": 1, "SlicesFirst": true, "Interval_ms": 0, "Time": "2019-12-18 21:36:42 -0500", "Prefix": "seq_4", "InitialPositionList": [ { "Label": "Pos0", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.21], "Focus": [17741.5], "XYStage": [ 44542.200000000004, 31636.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos1", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.22], "Focus": [17741.4], "XYStage": [ 43801.100000000006, 31637.9 ] }, "GridColumnIndex": 0 }, { "Label": "Pos2", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.5], "XYStage": [ 38561.3, 32040.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos3", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.185], "Focus": [17741.3], "XYStage": [ 37698.4, 32041.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos4", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.16], "Focus": [17741.5], "XYStage": [ 32375.4, 31758.9 ] }, "GridColumnIndex": 0 }, { "Label": "Pos5", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.17000000000002], "Focus": [17741.3], "XYStage": [ 31770.7, 31758.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos6", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.4], "XYStage": [ 26536.800000000003, 31068.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos7", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.205], "Focus": [17741.4], "XYStage": [ 25420.2, 31068.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos8", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.85], "Focus": [17741.3], "XYStage": [ 21059, 31508 ] }, "GridColumnIndex": 0 }, { "Label": "Pos9", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.82], "Focus": [17741.3], "XYStage": [ 19731.9, 31506.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos10", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.79], "Focus": [17741.4], "XYStage": [ 14528.5, 32007.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos11", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.83], "Focus": [17741.2], "XYStage": [ 13830.300000000001, 32009.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos12", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.79], "Focus": [17741.3], "XYStage": [ 8775.300000000001, 32017.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos13", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.815], "Focus": [17741.3], "XYStage": [ 8109.200000000001, 32008.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos14", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.805], "Focus": [17741.3], "XYStage": [ 3483.3, 31908.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos15", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.82], "Focus": [17741.2], "XYStage": [ 2686.8, 31903.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos16", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [211.48000000000002], "Focus": [17741.1], "XYStage": [ -1978.9, 31973.600000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos17", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [211.51500000000001], "Focus": [17741.1], "XYStage": [ -2957.8, 31982.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos18", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.195], "Focus": [17741.5], "XYStage": [ 47747.4, -576.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos19", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.23000000000002], "Focus": [17741.6], "XYStage": [ 47053.9, -576.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos20", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.205], "Focus": [17741.4], "XYStage": [ 41871.200000000004, -479.8 ] }, "GridColumnIndex": 0 }, { "Label": "Pos21", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.4], "XYStage": [ 40882.600000000006, -392 ] }, "GridColumnIndex": 0 }, { "Label": "Pos22", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.24], "Focus": [17741.4], "XYStage": [ 35905.6, -135.3 ] }, "GridColumnIndex": 0 }, { "Label": "Pos23", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.195], "Focus": [17741.5], "XYStage": [ 34990.9, -132.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos24", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.46], "Focus": [17741.4], "XYStage": [ 29678.100000000002, -958.1 ] }, "GridColumnIndex": 0 }, { "Label": "Pos25", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.44], "Focus": [17741.5], "XYStage": [ 28983.600000000002, -939.4000000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos26", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.475], "Focus": [17741.5], "XYStage": [ 23019.7, -122 ] }, "GridColumnIndex": 0 }, { "Label": "Pos27", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.44], "Focus": [17741.7], "XYStage": [ 22428, -116.80000000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos28", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.46], "Focus": [17741.7], "XYStage": [ 16364.900000000001, -181.10000000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos29", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.48000000000002], "Focus": [17741.5], "XYStage": [ 15712.1, -180 ] }, "GridColumnIndex": 0 }, { "Label": "Pos30", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.45000000000002], "Focus": [17741.7], "XYStage": [ 10346.1, 77.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos31", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.49], "Focus": [17741.4], "XYStage": [ 9755.5, 85.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos32", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.475], "Focus": [17741.5], "XYStage": [ 4775.5, 293.90000000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos33", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.445], "Focus": [17741.4], "XYStage": [ 3954.9, 293.90000000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos34", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.455], "Focus": [17741.4], "XYStage": [ -1324.9, 545.6 ] }, "GridColumnIndex": 0 }, { "Label": "Pos35", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.445], "Focus": [17741.3], "XYStage": [ -2097, 576.6 ] }, "GridColumnIndex": 0 } ], "z-step_um": 10, "GridRow": 0, "BitDepth": 16, "Depth": 2, "Channels": 6, "Source": "Micro-Manager", "ChColors": [ -16711681, -256, -65281, -1, -1, -16776961 ], "MetadataVersion": 10, "KeepShutterOpenChannels": true, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536, 65536 ], "CustomIntervals_ms": [], "Height": 1200, "MicroManagerVersion": "1.4.23 20180219", "GridColumn": 0, "UUID": "14a13bf7-c944-4672-b873-fcfbbb7ea13d", "PixelType": "GRAY16", "Width": 1200, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\Yan\\20191218-YC113-S7S8-miseq", "Frames": 1, "Comment": "", "UserName": "Nikon", "PixelSize_um": 1.1, "ROI": [ 0, 0, 1200, 1200 ], "Date": "2019-12-18", "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC", "P-DAPI-high" ], "KeepShutterOpenSlices": true, "IJType": 1, "ComputerName": "ZadorConfocal2", "Positions": 36, "PositionIndex": 9, "CameraTimeout": 20000, "TimeFirst": false }, "640 AO-Name": "AnalogIO", "Vincent-D3-Command": "Close" }, "FrameKey-0-5-5": { "COM4-StopBits": "1", "528 AO-MaxVolts": "5.0000", "XLIGHT Excitation Wheel-State": "1", "470 Shutter-Name": "AnalogIO", "Core-Shutter": "405", "555 AO-Demo": "No", "XLIGHT Dichroic-State": "2", "XYStage-StepSize-Y": "0.1000", "ZPositionUm": 248.82, "XYStage-StepSize-X": "0.1000", "PRIME-FirmwareVersion": "20.28", "PRIME-ClearCycles": "2", "520-Name": "DA Shutter", "405 Shutter-Name": "AnalogIO", "PIEZO-StepSize": "0.0050", "PRIME-ClearMode": "Pre-Exposure", "470 AO-MinVolts": "0.0000", "405 AO-SupportsTriggering": "1", "CameraChannelIndex": 0, "XYStage-StartSpeed": "500.0000", "COM9-StopBits": "2", "405 AO-Description": "NI DAC", "COM4-DelayBetweenCharsMs": "0.0000", "445 AO-Block voltage": "false", "520 AO-TriggerInputLine": "/Dev1/PFI0", "555 AO-Description": "NI DAC", "520 AO-TriggerSampleRate": "100000", "COM4-Verbose": "1", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM4-Name": "COM4", "405 Shutter-Volts": "5.0000", "Vincent-D3-Port": "COM1", "PRIME-PP 4 MIN ADU AFFECTED": "200", "470 Shutter-MinVolts": "0.0000", "PRIME-PP 3 ENABLED": "Yes", "470 AO-Description": "NI DAC", "PRIME-Description": "PVCAM API device adapter", "520 Shutter-MaxVolts": "5.0000", "520 Shutter-Volts": "0.0000", "528 AO-Sequenceable": "1", "PRIME-Binning": "1x1", "555 Shutter-MaxVolts": "5.0000", "528 Shutter-TriggerSequenceLength": "1000", "405 Shutter-Block voltage": "false", "XLIGHT Spin motor-State": "1", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "Vincent-D3-Time to open (ms)": "35.0000", "Vincent-D3-Time to close (ms)": "35.0000", "445 AO-TriggerSampleRate": "100000", "640 Shutter-IOChannel": "Dev1/ao14", "555 AO-Sequenceable": "1", "520 AO-Name": "AnalogIO", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "470 AO-Block voltage": "false", "COM1-Verbose": "1", "COM9-AnswerTimeout": "10000.0000", "528 Shutter-Name": "AnalogIO", "PRIME-Trigger Timeout (secs)": "10", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "NextFrame": 0, "470 AO-TriggerSequenceLength": "1000", "PRIME-TransposeXY": "0", "445 AO-TriggerSequenceLength": "1000", "COM1-Name": "COM1", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "COM9-DataBits": "8", "COM1-AnswerTimeout": "500.0000", "Camera": "", "TE2000-Version": "V2.50-V2.50-V2.10", "528 Shutter-Volts": "0.0000", "XLIGHT Hub-Name": "XLIGHT Hub", "PIEZO-Description": "Ludl stage driver adapter", "PositionName": "Pos9", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSampleRate": "100000", "PRIME-FanSpeedSetpoint": "High", "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "405 AO-TriggerSampleRate": "100000", "470 Shutter-Block voltage": "false", "470-State": "0", "COM3-Parity": "None", "405 AO-Volts": "4.5000", "PRIME-TransposeMirrorY": "0", "PRIME-ChipName": "GS144BSI", "470 AO-Volts": "0.0000", "PRIME-TransposeMirrorX": "0", "445 Shutter-SupportsTriggering": "0", "470 Shutter-SupportsTriggering": "0", "XLIGHT Hub-Description": "XLIGHT hub", "PRIME-PreampOffLimit": "10000", "470 AO-Sequenceable": "1", "528 Shutter-MaxVolts": "5.0000", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "Core-XYStage": "XYStage", "470 AO-TriggerSampleRate": "100000", "PRIME-Timing-PreTriggerDelayNs": "24672000.0000", "528-DA Device": "528 Shutter", "Focus-Description": "Nikon TE2000 focus adapter", "FilterBlock-State": "5", "520 AO-Demo": "No", "COM3-DelayBetweenCharsMs": "0.0000", "470 Shutter-Volts": "0.0000", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "PRIME-PP 2 THRESHOLD": "125", "PRIME-PP 4 THRESHOLD": "75", "XYStage-Acceleration": "4.0000", "470 Shutter-Demo": "No", "PRIME-Name": "PMPCIECam00", "640-DA Device": "640 Shutter", "405 AO-Block voltage": "false", "ElapsedTime-ms": 409823, "PRIME-Offset": "100", "445 AO-IOChannel": "Dev1/ao1", "640 AO-MaxVolts": "5.0000", "PositionIndex": 9, "PRIME-PP 1 ENABLED": "Yes", "COM1-BaudRate": "9600", "640-State": "0", "COM3-Verbose": "1", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "LudlController-TransmissionDelay": "0", "PRIME-FullWellCapacity": "62000", "PRIME-SMARTStreamingEnabled": "No", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "640 Shutter-Description": "NI DAC", "528-Description": "DA device that is used as a shutter", "COM4-Description": "Serial port driver (boost:asio)", "405 Shutter-Description": "NI DAC", "TE2000-Name": "TE2000", "528 Shutter-Description": "NI DAC", "COM9-Description": "Serial port driver (boost:asio)", "LudlController-Name": "LudlController", "445 Shutter-TriggerSequenceLength": "1000", "PRIME-SerialNumber": "A18B203008", "445 AO-Volts": "0.0000", "PRIME-X-dimension": "1200", "PRIME-ShutterCloseDelay": "0", "XLIGHT Disk slider-State": "2", "Position": "Default", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 AO-IOChannel": "Dev1/ao5", "520 AO-IOChannel": "Dev1/ao4", "528 AO-TriggerSequenceLength": "1000", "405 Shutter-MaxVolts": "5.0000", "470-DA Device": "470 Shutter", "PRIME-FTCapable": "No", "Core-ChannelGroup": "Channels", "555 AO-SupportsTriggering": "0", "PRIME-Y-dimension": "1200", "Frame": 0, "555 Shutter-Sequenceable": "1", "PRIME-Timing-ExposureTimeNs": "499998000.0000", "Core-ImageProcessor": "", "528-State": "0", "520 AO-Block voltage": "false", "XLIGHT Excitation Wheel-Label": "QUAD", "640 Shutter-Demo": "No", "COM3-StopBits": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "520 Shutter-IOChannel": "Dev1/ao11", "Width": 1200, "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-SupportsTriggering": "0", "640 AO-TriggerSampleRate": "100000", "520-Description": "DA device that is used as a shutter", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "405 Shutter-Sequenceable": "1", "405 Shutter-SupportsTriggering": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-TriggerSampleRate": "100000", "640 Shutter-MaxVolts": "5.0000", "555 AO-MaxVolts": "5.0000", "520 AO-TriggerSequenceLength": "1000", "COM3-BaudRate": "9600", "COM9-BaudRate": "9600", "Vincent-D3-Channel #": "Ch. #1", "PRIME-PMode": "Normal", "OpticalPath-Label": "Left(100)", "BitDepth": 16, "555 AO-TriggerSequenceLength": "1000", "445 Shutter-Description": "NI DAC", "405 AO-Sequenceable": "1", "PRIME-AcquisitionMethod": "Callbacks", "555 AO-Name": "AnalogIO", "PRIME-ShutterMode": "Pre-Exposure", "COM3-Handshaking": "Off", "445 AO-SupportsTriggering": "0", "ROI": "0-0-1200-1200", "COM9-Verbose": "1", "PRIME-Color": "OFF", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "PRIME-ShutterOpenDelay": "0", "COM1-Parity": "None", "Slice": 5, "SlicePosition": 0, "COM9-DelayBetweenCharsMs": "0.0000", "555-Name": "DA Shutter", "PIEZO-LudlSingleAxisName": "B", "XLIGHT Emission Wheel-Label": "QUAD", "640 Shutter-Name": "AnalogIO", "640 AO-Sequenceable": "1", "520 Shutter-Demo": "No", "528 Shutter-Demo": "No", "470-Name": "DA Shutter", "520-DA Device": "520 Shutter", "XYStage-Description": "Ludl XY stage driver adapter", "445 Shutter-MinVolts": "0.0000", "528 AO-TriggerSampleRate": "100000", "405 AO-MinVolts": "0.0000", "405 Shutter-TriggerSequenceLength": "1000", "Height": 1200, "555 AO-Block voltage": "false", "640 Shutter-Volts": "0.0000", "PixelType": "GRAY16", "445 Shutter-Block voltage": "false", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "520 Shutter-TriggerSequenceLength": "1000", "COM9-Name": "COM9", "Core-SLM": "", "555 AO-Volts": "0.0000", "555 Shutter-Block voltage": "false", "XYStage-Speed": "1800.0000", "520 AO-MaxVolts": "5.0000", "528 Shutter-SupportsTriggering": "0", "COM1-Description": "Serial port driver (boost:asio)", "PRIME-MetadataEnabled": "No", "520 Shutter-MinVolts": "0.0000", "470 Shutter-Sequenceable": "1", "PRIME-PreampDelay": "0", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-MinVolts": "0.0000", "ChannelIndex": 5, "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Core-AutoShutter": "1", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "Exposure-ms": 300, "PRIME-PP 2 ENABLED": "Yes", "640 Shutter-TriggerSampleRate": "100000", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "470 Shutter-IOChannel": "Dev1/ao10", "405 AO-Demo": "No", "PixelSizeUm": 1.1, "470 AO-Demo": "No", "470 Shutter-MaxVolts": "5.0000", "Vincent-D3-Name": "Vincent-D3", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FileName": "img_000000000_P-DAPI-high_005.tif", "LudlController-Reset": "Operate", "PRIME-Exposure": "300.00", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "445-Name": "DA Shutter", "555 Shutter-Name": "AnalogIO", "520 Shutter-TriggerSampleRate": "100000", "445 Shutter-IOChannel": "Dev1/ao9", "PRIME-TransposeCorrection": "0", "XYStage-StepSize": "0.1000", "445-Description": "DA device that is used as a shutter", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 AO-IOChannel": "Dev1/ao7", "520 Shutter-Name": "AnalogIO", "COM3-DataBits": "8", "640 AO-Volts": "0.0000", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "555 Shutter-IOChannel": "Dev1/ao13", "WaitInterval": null, "520 Shutter-Sequenceable": "1", "YPositionUm": 31506.2, "445-State": "0", "445-DA Device": "445 Shutter", "PRIME-CircularBufferAutoSize": "ON", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "555 AO-TriggerSampleRate": "100000", "445 Shutter-Volts": "0.0000", "XLIGHT Hub-Port": "COM3", "Core-AutoFocus": "", "COM4-Handshaking": "Off", "528 Shutter-MinVolts": "0.0000", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "445 Shutter-Name": "AnalogIO", "528 Shutter-Sequenceable": "1", "XYStage-TransposeMirrorX": "0", "XYStage-TransposeMirrorY": "0", "PRIME-ReadoutRate": "100MHz 16bit", "XYStage-Name": "Stage", "640 AO-SupportsTriggering": "0", "640-Name": "DA Shutter", "640 Shutter-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "640 AO-Block voltage": "false", "445 Shutter-Sequenceable": "1", "470 AO-MaxVolts": "5.0000", "COM4-BaudRate": "9600", "470-Description": "DA device that is used as a shutter", "520 Shutter-Description": "NI DAC", "PRIME-CCDTemperature": "-14.8500", "Time": "2019-12-18 21:37:17 -0500", "640 AO-TriggerSequenceLength": "1000", "470 AO-Name": "AnalogIO", "XLIGHT Disk slider-Label": "Disk Pos 40um", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "Binning": "1x1", "TE2000-ModelType": "1", "COM3-AnswerTimeout": "500.0000", "555 Shutter-SupportsTriggering": "0", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "TE2000-Port": "COM4", "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Description": "NI DAC", "COM4-AnswerTimeout": "500.0000", "COM1-DelayBetweenCharsMs": "0.0000", "FilterBlock-Label": "Confocal", "445 AO-Name": "AnalogIO", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-Timing-ReadoutTimeNs": "24672000.0000", "528 AO-Demo": "No", "520 AO-Sequenceable": "1", "555-Description": "DA device that is used as a shutter", "405-DA Device": "405 Shutter", "405 AO-TriggerSequenceLength": "1000", "640 AO-MinVolts": "0.0000", "640 Shutter-Block voltage": "false", "FrameIndex": 0, "PRIME-Port": "CMOS", "555 AO-MinVolts": "0.0000", "COM3-Description": "Serial port driver (boost:asio)", "528 AO-Description": "NI DAC", "555 AO-IOChannel": "Dev1/ao6", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "555 AO-TriggerInputLine": "/Dev1/PFI0", "PRIME-ActualInterval-ms": "390.5200", "LudlController-Port": "COM9", "445 Shutter-Demo": "No", "PRIME-Trigger-Expose Out-Mux": "1", "SliceIndex": 5, "PRIME-CCDTemperatureSetPoint": "-15.0000", "405 Shutter-MinVolts": "0.0000", "PRIME-ExposeOutMode": "First Row", "AxisPositions": null, "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Volts": "0.0000", "528-Name": "DA Shutter", "555-State": "0", "XLIGHT Dichroic-Label": "QUAD", "445 AO-Sequenceable": "1", "405-Name": "DA Shutter", "405 AO-IOChannel": "Dev1/ao0", "Core-Camera": "PRIME", "Focus-Name": "Focus", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "405 Shutter-IOChannel": "Dev1/ao8", "640 AO-Demo": "No", "640 Shutter-SupportsTriggering": "0", "445 AO-Demo": "No", "555-DA Device": "555 Shutter", "470 Shutter-Description": "NI DAC", "528 AO-Volts": "0.0000", "COM1-DataBits": "8", "Vincent-D3-Address": "x", "520 AO-SupportsTriggering": "0", "405-Description": "DA device that is used as a shutter", "640 Shutter-MinVolts": "0.0000", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "405 AO-Name": "AnalogIO", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "640 AO-TriggerInputLine": "/Dev1/PFI0", "405 AO-TriggerInputLine": "/Dev1/PFI0", "640-Description": "DA device that is used as a shutter", "445 Shutter-TriggerSampleRate": "100000", "470 Shutter-TriggerSequenceLength": "1000", "COM1-StopBits": "1", "520 Shutter-SupportsTriggering": "0", "405-State": "1", "Core-Initialize": "1", "XLIGHT Emission Wheel-State": "3", "520 AO-Description": "NI DAC", "528 AO-TriggerInputLine": "/Dev1/PFI0", "445 Shutter-MaxVolts": "5.0000", "COM9-Handshaking": "Off", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "XLIGHT Spin motor-Label": "On (spinning)", "PRIME-PP 5 ENABLED": "No", "UUID": "79a19e46-d856-4200-9749-cbe6cf2a65fe", "PRIME-TriggerMode": "Internal Trigger", "COM1-Handshaking": "Off", "PRIME-Timing-PostTriggerDelayNs": "49344000.0000", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "Focus-Position": "17741.5000", "528 Shutter-Block voltage": "false", "Channel": "P-DAPI-high", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "555 Shutter-Demo": "No", "405 Shutter-Demo": "No", "528 AO-Name": "AnalogIO", "520-State": "0", "Core-Galvo": "", "405 Shutter-TriggerSampleRate": "100000", "Focus-FocusStepSize_(nm)": "100", "PRIME-PixelType": "16bit", "COM4-DataBits": "8", "470 AO-IOChannel": "Dev1/ao2", "520 AO-MinVolts": "0.0000", "COM9-Parity": "None", "PRIME-CircularBufferEnabled": "ON", "PRIME-PP 6 Reset": "No", "640 AO-Description": "NI DAC", "Source": "PRIME", "445 AO-MinVolts": "0.0000", "PIEZO-Name": "Stage", "445 AO-Description": "NI DAC", "PRIME-CircularBufferFrameCount": "46", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "520 AO-Volts": "0.0000", "528 Shutter-TriggerSampleRate": "100000", "XPositionUm": 19731.9, "OpticalPath-State": "4", "PRIME-PP 3 THRESHOLD": "80", "PRIME-PVCAM Version": "3.7.1", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Gain": "1-HDR", "640 Shutter-TriggerSequenceLength": "1000", "528 AO-SupportsTriggering": "0", "445 AO-TriggerInputLine": "/Dev1/PFI0", "OpticalPath-Name": "OpticalPath", "PRIME-PP 4 ENABLED": "Yes", "PRIME-PVCAM Adapter Version": "1.3.29", "COM3-Name": "COM3", "405 AO-MaxVolts": "5.0000", "520 Shutter-Block voltage": "false", "PRIME-Timing-ClearingTimeNs": "49344000.0000", "Core-Focus": "PIEZO", "528 AO-Block voltage": "false", "TE2000-Description": "Nikon TE2000 hub", "528 AO-MinVolts": "0.0000", "COM4-Parity": "None", "Summary": { "Slices": 11, "ChContrastMin": [ 0, 0, 0, 0, 0, 0 ], "PixelAspect": 1, "SlicesFirst": true, "Interval_ms": 0, "Time": "2019-12-18 21:36:42 -0500", "Prefix": "seq_4", "InitialPositionList": [ { "Label": "Pos0", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.21], "Focus": [17741.5], "XYStage": [ 44542.200000000004, 31636.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos1", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.22], "Focus": [17741.4], "XYStage": [ 43801.100000000006, 31637.9 ] }, "GridColumnIndex": 0 }, { "Label": "Pos2", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.5], "XYStage": [ 38561.3, 32040.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos3", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.185], "Focus": [17741.3], "XYStage": [ 37698.4, 32041.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos4", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.16], "Focus": [17741.5], "XYStage": [ 32375.4, 31758.9 ] }, "GridColumnIndex": 0 }, { "Label": "Pos5", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.17000000000002], "Focus": [17741.3], "XYStage": [ 31770.7, 31758.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos6", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.4], "XYStage": [ 26536.800000000003, 31068.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos7", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.205], "Focus": [17741.4], "XYStage": [ 25420.2, 31068.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos8", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.85], "Focus": [17741.3], "XYStage": [ 21059, 31508 ] }, "GridColumnIndex": 0 }, { "Label": "Pos9", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.82], "Focus": [17741.3], "XYStage": [ 19731.9, 31506.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos10", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.79], "Focus": [17741.4], "XYStage": [ 14528.5, 32007.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos11", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.83], "Focus": [17741.2], "XYStage": [ 13830.300000000001, 32009.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos12", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.79], "Focus": [17741.3], "XYStage": [ 8775.300000000001, 32017.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos13", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.815], "Focus": [17741.3], "XYStage": [ 8109.200000000001, 32008.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos14", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.805], "Focus": [17741.3], "XYStage": [ 3483.3, 31908.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos15", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.82], "Focus": [17741.2], "XYStage": [ 2686.8, 31903.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos16", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [211.48000000000002], "Focus": [17741.1], "XYStage": [ -1978.9, 31973.600000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos17", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [211.51500000000001], "Focus": [17741.1], "XYStage": [ -2957.8, 31982.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos18", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.195], "Focus": [17741.5], "XYStage": [ 47747.4, -576.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos19", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.23000000000002], "Focus": [17741.6], "XYStage": [ 47053.9, -576.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos20", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.205], "Focus": [17741.4], "XYStage": [ 41871.200000000004, -479.8 ] }, "GridColumnIndex": 0 }, { "Label": "Pos21", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.4], "XYStage": [ 40882.600000000006, -392 ] }, "GridColumnIndex": 0 }, { "Label": "Pos22", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.24], "Focus": [17741.4], "XYStage": [ 35905.6, -135.3 ] }, "GridColumnIndex": 0 }, { "Label": "Pos23", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.195], "Focus": [17741.5], "XYStage": [ 34990.9, -132.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos24", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.46], "Focus": [17741.4], "XYStage": [ 29678.100000000002, -958.1 ] }, "GridColumnIndex": 0 }, { "Label": "Pos25", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.44], "Focus": [17741.5], "XYStage": [ 28983.600000000002, -939.4000000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos26", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.475], "Focus": [17741.5], "XYStage": [ 23019.7, -122 ] }, "GridColumnIndex": 0 }, { "Label": "Pos27", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.44], "Focus": [17741.7], "XYStage": [ 22428, -116.80000000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos28", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.46], "Focus": [17741.7], "XYStage": [ 16364.900000000001, -181.10000000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos29", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.48000000000002], "Focus": [17741.5], "XYStage": [ 15712.1, -180 ] }, "GridColumnIndex": 0 }, { "Label": "Pos30", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.45000000000002], "Focus": [17741.7], "XYStage": [ 10346.1, 77.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos31", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.49], "Focus": [17741.4], "XYStage": [ 9755.5, 85.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos32", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.475], "Focus": [17741.5], "XYStage": [ 4775.5, 293.90000000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos33", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.445], "Focus": [17741.4], "XYStage": [ 3954.9, 293.90000000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos34", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.455], "Focus": [17741.4], "XYStage": [ -1324.9, 545.6 ] }, "GridColumnIndex": 0 }, { "Label": "Pos35", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.445], "Focus": [17741.3], "XYStage": [ -2097, 576.6 ] }, "GridColumnIndex": 0 } ], "z-step_um": 10, "GridRow": 0, "BitDepth": 16, "Depth": 2, "Channels": 6, "Source": "Micro-Manager", "ChColors": [ -16711681, -256, -65281, -1, -1, -16776961 ], "MetadataVersion": 10, "KeepShutterOpenChannels": true, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536, 65536 ], "CustomIntervals_ms": [], "Height": 1200, "MicroManagerVersion": "1.4.23 20180219", "GridColumn": 0, "UUID": "14a13bf7-c944-4672-b873-fcfbbb7ea13d", "PixelType": "GRAY16", "Width": 1200, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\Yan\\20191218-YC113-S7S8-miseq", "Frames": 1, "Comment": "", "UserName": "Nikon", "PixelSize_um": 1.1, "ROI": [ 0, 0, 1200, 1200 ], "Date": "2019-12-18", "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC", "P-DAPI-high" ], "KeepShutterOpenSlices": true, "IJType": 1, "ComputerName": "ZadorConfocal2", "Positions": 36, "PositionIndex": 9, "CameraTimeout": 20000, "TimeFirst": false }, "640 AO-Name": "AnalogIO", "Vincent-D3-Command": "Close" }, "FrameKey-0-5-6": { "COM4-StopBits": "1", "528 AO-MaxVolts": "5.0000", "XLIGHT Excitation Wheel-State": "1", "470 Shutter-Name": "AnalogIO", "Core-Shutter": "405", "555 AO-Demo": "No", "XLIGHT Dichroic-State": "2", "XYStage-StepSize-Y": "0.1000", "ZPositionUm": 258.82, "XYStage-StepSize-X": "0.1000", "PRIME-FirmwareVersion": "20.28", "PRIME-ClearCycles": "2", "520-Name": "DA Shutter", "405 Shutter-Name": "AnalogIO", "PIEZO-StepSize": "0.0050", "PRIME-ClearMode": "Pre-Exposure", "470 AO-MinVolts": "0.0000", "405 AO-SupportsTriggering": "1", "CameraChannelIndex": 0, "XYStage-StartSpeed": "500.0000", "COM9-StopBits": "2", "405 AO-Description": "NI DAC", "COM4-DelayBetweenCharsMs": "0.0000", "445 AO-Block voltage": "false", "520 AO-TriggerInputLine": "/Dev1/PFI0", "555 AO-Description": "NI DAC", "520 AO-TriggerSampleRate": "100000", "COM4-Verbose": "1", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM4-Name": "COM4", "405 Shutter-Volts": "5.0000", "Vincent-D3-Port": "COM1", "PRIME-PP 4 MIN ADU AFFECTED": "200", "470 Shutter-MinVolts": "0.0000", "PRIME-PP 3 ENABLED": "Yes", "470 AO-Description": "NI DAC", "PRIME-Description": "PVCAM API device adapter", "520 Shutter-MaxVolts": "5.0000", "520 Shutter-Volts": "0.0000", "528 AO-Sequenceable": "1", "PRIME-Binning": "1x1", "555 Shutter-MaxVolts": "5.0000", "528 Shutter-TriggerSequenceLength": "1000", "405 Shutter-Block voltage": "false", "XLIGHT Spin motor-State": "1", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "Vincent-D3-Time to open (ms)": "35.0000", "Vincent-D3-Time to close (ms)": "35.0000", "445 AO-TriggerSampleRate": "100000", "640 Shutter-IOChannel": "Dev1/ao14", "555 AO-Sequenceable": "1", "520 AO-Name": "AnalogIO", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "470 AO-Block voltage": "false", "COM1-Verbose": "1", "COM9-AnswerTimeout": "10000.0000", "528 Shutter-Name": "AnalogIO", "PRIME-Trigger Timeout (secs)": "10", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "NextFrame": 0, "470 AO-TriggerSequenceLength": "1000", "PRIME-TransposeXY": "0", "445 AO-TriggerSequenceLength": "1000", "COM1-Name": "COM1", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "COM9-DataBits": "8", "COM1-AnswerTimeout": "500.0000", "Camera": "", "TE2000-Version": "V2.50-V2.50-V2.10", "528 Shutter-Volts": "0.0000", "XLIGHT Hub-Name": "XLIGHT Hub", "PIEZO-Description": "Ludl stage driver adapter", "PositionName": "Pos9", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSampleRate": "100000", "PRIME-FanSpeedSetpoint": "High", "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "405 AO-TriggerSampleRate": "100000", "470 Shutter-Block voltage": "false", "470-State": "0", "COM3-Parity": "None", "405 AO-Volts": "4.5000", "PRIME-TransposeMirrorY": "0", "PRIME-ChipName": "GS144BSI", "470 AO-Volts": "0.0000", "PRIME-TransposeMirrorX": "0", "445 Shutter-SupportsTriggering": "0", "470 Shutter-SupportsTriggering": "0", "XLIGHT Hub-Description": "XLIGHT hub", "PRIME-PreampOffLimit": "10000", "470 AO-Sequenceable": "1", "528 Shutter-MaxVolts": "5.0000", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "Core-XYStage": "XYStage", "470 AO-TriggerSampleRate": "100000", "PRIME-Timing-PreTriggerDelayNs": "24672000.0000", "528-DA Device": "528 Shutter", "Focus-Description": "Nikon TE2000 focus adapter", "FilterBlock-State": "5", "520 AO-Demo": "No", "COM3-DelayBetweenCharsMs": "0.0000", "470 Shutter-Volts": "0.0000", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "PRIME-PP 2 THRESHOLD": "125", "PRIME-PP 4 THRESHOLD": "75", "XYStage-Acceleration": "4.0000", "470 Shutter-Demo": "No", "PRIME-Name": "PMPCIECam00", "640-DA Device": "640 Shutter", "405 AO-Block voltage": "false", "ElapsedTime-ms": 410366, "PRIME-Offset": "100", "445 AO-IOChannel": "Dev1/ao1", "640 AO-MaxVolts": "5.0000", "PositionIndex": 9, "PRIME-PP 1 ENABLED": "Yes", "COM1-BaudRate": "9600", "640-State": "0", "COM3-Verbose": "1", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "LudlController-TransmissionDelay": "0", "PRIME-FullWellCapacity": "62000", "PRIME-SMARTStreamingEnabled": "No", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "640 Shutter-Description": "NI DAC", "528-Description": "DA device that is used as a shutter", "COM4-Description": "Serial port driver (boost:asio)", "405 Shutter-Description": "NI DAC", "TE2000-Name": "TE2000", "528 Shutter-Description": "NI DAC", "COM9-Description": "Serial port driver (boost:asio)", "LudlController-Name": "LudlController", "445 Shutter-TriggerSequenceLength": "1000", "PRIME-SerialNumber": "A18B203008", "445 AO-Volts": "0.0000", "PRIME-X-dimension": "1200", "PRIME-ShutterCloseDelay": "0", "XLIGHT Disk slider-State": "2", "Position": "Default", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 AO-IOChannel": "Dev1/ao5", "520 AO-IOChannel": "Dev1/ao4", "528 AO-TriggerSequenceLength": "1000", "405 Shutter-MaxVolts": "5.0000", "470-DA Device": "470 Shutter", "PRIME-FTCapable": "No", "Core-ChannelGroup": "Channels", "555 AO-SupportsTriggering": "0", "PRIME-Y-dimension": "1200", "Frame": 0, "555 Shutter-Sequenceable": "1", "PRIME-Timing-ExposureTimeNs": "499998000.0000", "Core-ImageProcessor": "", "528-State": "0", "520 AO-Block voltage": "false", "XLIGHT Excitation Wheel-Label": "QUAD", "640 Shutter-Demo": "No", "COM3-StopBits": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "520 Shutter-IOChannel": "Dev1/ao11", "Width": 1200, "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-SupportsTriggering": "0", "640 AO-TriggerSampleRate": "100000", "520-Description": "DA device that is used as a shutter", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "405 Shutter-Sequenceable": "1", "405 Shutter-SupportsTriggering": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-TriggerSampleRate": "100000", "640 Shutter-MaxVolts": "5.0000", "555 AO-MaxVolts": "5.0000", "520 AO-TriggerSequenceLength": "1000", "COM3-BaudRate": "9600", "COM9-BaudRate": "9600", "Vincent-D3-Channel #": "Ch. #1", "PRIME-PMode": "Normal", "OpticalPath-Label": "Left(100)", "BitDepth": 16, "555 AO-TriggerSequenceLength": "1000", "445 Shutter-Description": "NI DAC", "405 AO-Sequenceable": "1", "PRIME-AcquisitionMethod": "Callbacks", "555 AO-Name": "AnalogIO", "PRIME-ShutterMode": "Pre-Exposure", "COM3-Handshaking": "Off", "445 AO-SupportsTriggering": "0", "ROI": "0-0-1200-1200", "COM9-Verbose": "1", "PRIME-Color": "OFF", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "PRIME-ShutterOpenDelay": "0", "COM1-Parity": "None", "Slice": 6, "SlicePosition": 10, "COM9-DelayBetweenCharsMs": "0.0000", "555-Name": "DA Shutter", "PIEZO-LudlSingleAxisName": "B", "XLIGHT Emission Wheel-Label": "QUAD", "640 Shutter-Name": "AnalogIO", "640 AO-Sequenceable": "1", "520 Shutter-Demo": "No", "528 Shutter-Demo": "No", "470-Name": "DA Shutter", "520-DA Device": "520 Shutter", "XYStage-Description": "Ludl XY stage driver adapter", "445 Shutter-MinVolts": "0.0000", "528 AO-TriggerSampleRate": "100000", "405 AO-MinVolts": "0.0000", "405 Shutter-TriggerSequenceLength": "1000", "Height": 1200, "555 AO-Block voltage": "false", "640 Shutter-Volts": "0.0000", "PixelType": "GRAY16", "445 Shutter-Block voltage": "false", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "520 Shutter-TriggerSequenceLength": "1000", "COM9-Name": "COM9", "Core-SLM": "", "555 AO-Volts": "0.0000", "555 Shutter-Block voltage": "false", "XYStage-Speed": "1800.0000", "520 AO-MaxVolts": "5.0000", "528 Shutter-SupportsTriggering": "0", "COM1-Description": "Serial port driver (boost:asio)", "PRIME-MetadataEnabled": "No", "520 Shutter-MinVolts": "0.0000", "470 Shutter-Sequenceable": "1", "PRIME-PreampDelay": "0", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-MinVolts": "0.0000", "ChannelIndex": 5, "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Core-AutoShutter": "1", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "Exposure-ms": 300, "PRIME-PP 2 ENABLED": "Yes", "640 Shutter-TriggerSampleRate": "100000", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "470 Shutter-IOChannel": "Dev1/ao10", "405 AO-Demo": "No", "PixelSizeUm": 1.1, "470 AO-Demo": "No", "470 Shutter-MaxVolts": "5.0000", "Vincent-D3-Name": "Vincent-D3", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FileName": "img_000000000_P-DAPI-high_006.tif", "LudlController-Reset": "Operate", "PRIME-Exposure": "300.00", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "445-Name": "DA Shutter", "555 Shutter-Name": "AnalogIO", "520 Shutter-TriggerSampleRate": "100000", "445 Shutter-IOChannel": "Dev1/ao9", "PRIME-TransposeCorrection": "0", "XYStage-StepSize": "0.1000", "445-Description": "DA device that is used as a shutter", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 AO-IOChannel": "Dev1/ao7", "520 Shutter-Name": "AnalogIO", "COM3-DataBits": "8", "640 AO-Volts": "0.0000", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "555 Shutter-IOChannel": "Dev1/ao13", "WaitInterval": null, "520 Shutter-Sequenceable": "1", "YPositionUm": 31506.2, "445-State": "0", "445-DA Device": "445 Shutter", "PRIME-CircularBufferAutoSize": "ON", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "555 AO-TriggerSampleRate": "100000", "445 Shutter-Volts": "0.0000", "XLIGHT Hub-Port": "COM3", "Core-AutoFocus": "", "COM4-Handshaking": "Off", "528 Shutter-MinVolts": "0.0000", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "445 Shutter-Name": "AnalogIO", "528 Shutter-Sequenceable": "1", "XYStage-TransposeMirrorX": "0", "XYStage-TransposeMirrorY": "0", "PRIME-ReadoutRate": "100MHz 16bit", "XYStage-Name": "Stage", "640 AO-SupportsTriggering": "0", "640-Name": "DA Shutter", "640 Shutter-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "640 AO-Block voltage": "false", "445 Shutter-Sequenceable": "1", "470 AO-MaxVolts": "5.0000", "COM4-BaudRate": "9600", "470-Description": "DA device that is used as a shutter", "520 Shutter-Description": "NI DAC", "PRIME-CCDTemperature": "-14.8500", "Time": "2019-12-18 21:37:18 -0500", "640 AO-TriggerSequenceLength": "1000", "470 AO-Name": "AnalogIO", "XLIGHT Disk slider-Label": "Disk Pos 40um", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "Binning": "1x1", "TE2000-ModelType": "1", "COM3-AnswerTimeout": "500.0000", "555 Shutter-SupportsTriggering": "0", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "TE2000-Port": "COM4", "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Description": "NI DAC", "COM4-AnswerTimeout": "500.0000", "COM1-DelayBetweenCharsMs": "0.0000", "FilterBlock-Label": "Confocal", "445 AO-Name": "AnalogIO", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-Timing-ReadoutTimeNs": "24672000.0000", "528 AO-Demo": "No", "520 AO-Sequenceable": "1", "555-Description": "DA device that is used as a shutter", "405-DA Device": "405 Shutter", "405 AO-TriggerSequenceLength": "1000", "640 AO-MinVolts": "0.0000", "640 Shutter-Block voltage": "false", "FrameIndex": 0, "PRIME-Port": "CMOS", "555 AO-MinVolts": "0.0000", "COM3-Description": "Serial port driver (boost:asio)", "528 AO-Description": "NI DAC", "555 AO-IOChannel": "Dev1/ao6", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "555 AO-TriggerInputLine": "/Dev1/PFI0", "PRIME-ActualInterval-ms": "390.5200", "LudlController-Port": "COM9", "445 Shutter-Demo": "No", "PRIME-Trigger-Expose Out-Mux": "1", "SliceIndex": 6, "PRIME-CCDTemperatureSetPoint": "-15.0000", "405 Shutter-MinVolts": "0.0000", "PRIME-ExposeOutMode": "First Row", "AxisPositions": null, "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Volts": "0.0000", "528-Name": "DA Shutter", "555-State": "0", "XLIGHT Dichroic-Label": "QUAD", "445 AO-Sequenceable": "1", "405-Name": "DA Shutter", "405 AO-IOChannel": "Dev1/ao0", "Core-Camera": "PRIME", "Focus-Name": "Focus", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "405 Shutter-IOChannel": "Dev1/ao8", "640 AO-Demo": "No", "640 Shutter-SupportsTriggering": "0", "445 AO-Demo": "No", "555-DA Device": "555 Shutter", "470 Shutter-Description": "NI DAC", "528 AO-Volts": "0.0000", "COM1-DataBits": "8", "Vincent-D3-Address": "x", "520 AO-SupportsTriggering": "0", "405-Description": "DA device that is used as a shutter", "640 Shutter-MinVolts": "0.0000", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "405 AO-Name": "AnalogIO", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "640 AO-TriggerInputLine": "/Dev1/PFI0", "405 AO-TriggerInputLine": "/Dev1/PFI0", "640-Description": "DA device that is used as a shutter", "445 Shutter-TriggerSampleRate": "100000", "470 Shutter-TriggerSequenceLength": "1000", "COM1-StopBits": "1", "520 Shutter-SupportsTriggering": "0", "405-State": "1", "Core-Initialize": "1", "XLIGHT Emission Wheel-State": "3", "520 AO-Description": "NI DAC", "528 AO-TriggerInputLine": "/Dev1/PFI0", "445 Shutter-MaxVolts": "5.0000", "COM9-Handshaking": "Off", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "XLIGHT Spin motor-Label": "On (spinning)", "PRIME-PP 5 ENABLED": "No", "UUID": "f59da2ef-ff25-49b9-8527-f8beb5aed5de", "PRIME-TriggerMode": "Internal Trigger", "COM1-Handshaking": "Off", "PRIME-Timing-PostTriggerDelayNs": "49344000.0000", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "Focus-Position": "17741.5000", "528 Shutter-Block voltage": "false", "Channel": "P-DAPI-high", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "555 Shutter-Demo": "No", "405 Shutter-Demo": "No", "528 AO-Name": "AnalogIO", "520-State": "0", "Core-Galvo": "", "405 Shutter-TriggerSampleRate": "100000", "Focus-FocusStepSize_(nm)": "100", "PRIME-PixelType": "16bit", "COM4-DataBits": "8", "470 AO-IOChannel": "Dev1/ao2", "520 AO-MinVolts": "0.0000", "COM9-Parity": "None", "PRIME-CircularBufferEnabled": "ON", "PRIME-PP 6 Reset": "No", "640 AO-Description": "NI DAC", "Source": "PRIME", "445 AO-MinVolts": "0.0000", "PIEZO-Name": "Stage", "445 AO-Description": "NI DAC", "PRIME-CircularBufferFrameCount": "46", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "520 AO-Volts": "0.0000", "528 Shutter-TriggerSampleRate": "100000", "XPositionUm": 19731.9, "OpticalPath-State": "4", "PRIME-PP 3 THRESHOLD": "80", "PRIME-PVCAM Version": "3.7.1", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Gain": "1-HDR", "640 Shutter-TriggerSequenceLength": "1000", "528 AO-SupportsTriggering": "0", "445 AO-TriggerInputLine": "/Dev1/PFI0", "OpticalPath-Name": "OpticalPath", "PRIME-PP 4 ENABLED": "Yes", "PRIME-PVCAM Adapter Version": "1.3.29", "COM3-Name": "COM3", "405 AO-MaxVolts": "5.0000", "520 Shutter-Block voltage": "false", "PRIME-Timing-ClearingTimeNs": "49344000.0000", "Core-Focus": "PIEZO", "528 AO-Block voltage": "false", "TE2000-Description": "Nikon TE2000 hub", "528 AO-MinVolts": "0.0000", "COM4-Parity": "None", "Summary": { "Slices": 11, "ChContrastMin": [ 0, 0, 0, 0, 0, 0 ], "PixelAspect": 1, "SlicesFirst": true, "Interval_ms": 0, "Time": "2019-12-18 21:36:42 -0500", "Prefix": "seq_4", "InitialPositionList": [ { "Label": "Pos0", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.21], "Focus": [17741.5], "XYStage": [ 44542.200000000004, 31636.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos1", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.22], "Focus": [17741.4], "XYStage": [ 43801.100000000006, 31637.9 ] }, "GridColumnIndex": 0 }, { "Label": "Pos2", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.5], "XYStage": [ 38561.3, 32040.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos3", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.185], "Focus": [17741.3], "XYStage": [ 37698.4, 32041.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos4", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.16], "Focus": [17741.5], "XYStage": [ 32375.4, 31758.9 ] }, "GridColumnIndex": 0 }, { "Label": "Pos5", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.17000000000002], "Focus": [17741.3], "XYStage": [ 31770.7, 31758.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos6", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.4], "XYStage": [ 26536.800000000003, 31068.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos7", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.205], "Focus": [17741.4], "XYStage": [ 25420.2, 31068.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos8", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.85], "Focus": [17741.3], "XYStage": [ 21059, 31508 ] }, "GridColumnIndex": 0 }, { "Label": "Pos9", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.82], "Focus": [17741.3], "XYStage": [ 19731.9, 31506.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos10", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.79], "Focus": [17741.4], "XYStage": [ 14528.5, 32007.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos11", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.83], "Focus": [17741.2], "XYStage": [ 13830.300000000001, 32009.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos12", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.79], "Focus": [17741.3], "XYStage": [ 8775.300000000001, 32017.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos13", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.815], "Focus": [17741.3], "XYStage": [ 8109.200000000001, 32008.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos14", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.805], "Focus": [17741.3], "XYStage": [ 3483.3, 31908.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos15", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.82], "Focus": [17741.2], "XYStage": [ 2686.8, 31903.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos16", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [211.48000000000002], "Focus": [17741.1], "XYStage": [ -1978.9, 31973.600000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos17", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [211.51500000000001], "Focus": [17741.1], "XYStage": [ -2957.8, 31982.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos18", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.195], "Focus": [17741.5], "XYStage": [ 47747.4, -576.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos19", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.23000000000002], "Focus": [17741.6], "XYStage": [ 47053.9, -576.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos20", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.205], "Focus": [17741.4], "XYStage": [ 41871.200000000004, -479.8 ] }, "GridColumnIndex": 0 }, { "Label": "Pos21", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.4], "XYStage": [ 40882.600000000006, -392 ] }, "GridColumnIndex": 0 }, { "Label": "Pos22", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.24], "Focus": [17741.4], "XYStage": [ 35905.6, -135.3 ] }, "GridColumnIndex": 0 }, { "Label": "Pos23", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.195], "Focus": [17741.5], "XYStage": [ 34990.9, -132.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos24", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.46], "Focus": [17741.4], "XYStage": [ 29678.100000000002, -958.1 ] }, "GridColumnIndex": 0 }, { "Label": "Pos25", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.44], "Focus": [17741.5], "XYStage": [ 28983.600000000002, -939.4000000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos26", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.475], "Focus": [17741.5], "XYStage": [ 23019.7, -122 ] }, "GridColumnIndex": 0 }, { "Label": "Pos27", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.44], "Focus": [17741.7], "XYStage": [ 22428, -116.80000000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos28", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.46], "Focus": [17741.7], "XYStage": [ 16364.900000000001, -181.10000000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos29", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.48000000000002], "Focus": [17741.5], "XYStage": [ 15712.1, -180 ] }, "GridColumnIndex": 0 }, { "Label": "Pos30", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.45000000000002], "Focus": [17741.7], "XYStage": [ 10346.1, 77.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos31", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.49], "Focus": [17741.4], "XYStage": [ 9755.5, 85.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos32", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.475], "Focus": [17741.5], "XYStage": [ 4775.5, 293.90000000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos33", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.445], "Focus": [17741.4], "XYStage": [ 3954.9, 293.90000000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos34", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.455], "Focus": [17741.4], "XYStage": [ -1324.9, 545.6 ] }, "GridColumnIndex": 0 }, { "Label": "Pos35", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.445], "Focus": [17741.3], "XYStage": [ -2097, 576.6 ] }, "GridColumnIndex": 0 } ], "z-step_um": 10, "GridRow": 0, "BitDepth": 16, "Depth": 2, "Channels": 6, "Source": "Micro-Manager", "ChColors": [ -16711681, -256, -65281, -1, -1, -16776961 ], "MetadataVersion": 10, "KeepShutterOpenChannels": true, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536, 65536 ], "CustomIntervals_ms": [], "Height": 1200, "MicroManagerVersion": "1.4.23 20180219", "GridColumn": 0, "UUID": "14a13bf7-c944-4672-b873-fcfbbb7ea13d", "PixelType": "GRAY16", "Width": 1200, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\Yan\\20191218-YC113-S7S8-miseq", "Frames": 1, "Comment": "", "UserName": "Nikon", "PixelSize_um": 1.1, "ROI": [ 0, 0, 1200, 1200 ], "Date": "2019-12-18", "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC", "P-DAPI-high" ], "KeepShutterOpenSlices": true, "IJType": 1, "ComputerName": "ZadorConfocal2", "Positions": 36, "PositionIndex": 9, "CameraTimeout": 20000, "TimeFirst": false }, "640 AO-Name": "AnalogIO", "Vincent-D3-Command": "Close" }, "FrameKey-0-5-7": { "COM4-StopBits": "1", "528 AO-MaxVolts": "5.0000", "XLIGHT Excitation Wheel-State": "1", "470 Shutter-Name": "AnalogIO", "Core-Shutter": "405", "555 AO-Demo": "No", "XLIGHT Dichroic-State": "2", "XYStage-StepSize-Y": "0.1000", "ZPositionUm": 268.82, "XYStage-StepSize-X": "0.1000", "PRIME-FirmwareVersion": "20.28", "PRIME-ClearCycles": "2", "520-Name": "DA Shutter", "405 Shutter-Name": "AnalogIO", "PIEZO-StepSize": "0.0050", "PRIME-ClearMode": "Pre-Exposure", "470 AO-MinVolts": "0.0000", "405 AO-SupportsTriggering": "1", "CameraChannelIndex": 0, "XYStage-StartSpeed": "500.0000", "COM9-StopBits": "2", "405 AO-Description": "NI DAC", "COM4-DelayBetweenCharsMs": "0.0000", "445 AO-Block voltage": "false", "520 AO-TriggerInputLine": "/Dev1/PFI0", "555 AO-Description": "NI DAC", "520 AO-TriggerSampleRate": "100000", "COM4-Verbose": "1", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM4-Name": "COM4", "405 Shutter-Volts": "5.0000", "Vincent-D3-Port": "COM1", "PRIME-PP 4 MIN ADU AFFECTED": "200", "470 Shutter-MinVolts": "0.0000", "PRIME-PP 3 ENABLED": "Yes", "470 AO-Description": "NI DAC", "PRIME-Description": "PVCAM API device adapter", "520 Shutter-MaxVolts": "5.0000", "520 Shutter-Volts": "0.0000", "528 AO-Sequenceable": "1", "PRIME-Binning": "1x1", "555 Shutter-MaxVolts": "5.0000", "528 Shutter-TriggerSequenceLength": "1000", "405 Shutter-Block voltage": "false", "XLIGHT Spin motor-State": "1", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "Vincent-D3-Time to open (ms)": "35.0000", "Vincent-D3-Time to close (ms)": "35.0000", "445 AO-TriggerSampleRate": "100000", "640 Shutter-IOChannel": "Dev1/ao14", "555 AO-Sequenceable": "1", "520 AO-Name": "AnalogIO", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "470 AO-Block voltage": "false", "COM1-Verbose": "1", "COM9-AnswerTimeout": "10000.0000", "528 Shutter-Name": "AnalogIO", "PRIME-Trigger Timeout (secs)": "10", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "NextFrame": 0, "470 AO-TriggerSequenceLength": "1000", "PRIME-TransposeXY": "0", "445 AO-TriggerSequenceLength": "1000", "COM1-Name": "COM1", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "COM9-DataBits": "8", "COM1-AnswerTimeout": "500.0000", "Camera": "", "TE2000-Version": "V2.50-V2.50-V2.10", "528 Shutter-Volts": "0.0000", "XLIGHT Hub-Name": "XLIGHT Hub", "PIEZO-Description": "Ludl stage driver adapter", "PositionName": "Pos9", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSampleRate": "100000", "PRIME-FanSpeedSetpoint": "High", "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "405 AO-TriggerSampleRate": "100000", "470 Shutter-Block voltage": "false", "470-State": "0", "COM3-Parity": "None", "405 AO-Volts": "4.5000", "PRIME-TransposeMirrorY": "0", "PRIME-ChipName": "GS144BSI", "470 AO-Volts": "0.0000", "PRIME-TransposeMirrorX": "0", "445 Shutter-SupportsTriggering": "0", "470 Shutter-SupportsTriggering": "0", "XLIGHT Hub-Description": "XLIGHT hub", "PRIME-PreampOffLimit": "10000", "470 AO-Sequenceable": "1", "528 Shutter-MaxVolts": "5.0000", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "Core-XYStage": "XYStage", "470 AO-TriggerSampleRate": "100000", "PRIME-Timing-PreTriggerDelayNs": "24672000.0000", "528-DA Device": "528 Shutter", "Focus-Description": "Nikon TE2000 focus adapter", "FilterBlock-State": "5", "520 AO-Demo": "No", "COM3-DelayBetweenCharsMs": "0.0000", "470 Shutter-Volts": "0.0000", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "PRIME-PP 2 THRESHOLD": "125", "PRIME-PP 4 THRESHOLD": "75", "XYStage-Acceleration": "4.0000", "470 Shutter-Demo": "No", "PRIME-Name": "PMPCIECam00", "640-DA Device": "640 Shutter", "405 AO-Block voltage": "false", "ElapsedTime-ms": 410912, "PRIME-Offset": "100", "445 AO-IOChannel": "Dev1/ao1", "640 AO-MaxVolts": "5.0000", "PositionIndex": 9, "PRIME-PP 1 ENABLED": "Yes", "COM1-BaudRate": "9600", "640-State": "0", "COM3-Verbose": "1", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "LudlController-TransmissionDelay": "0", "PRIME-FullWellCapacity": "62000", "PRIME-SMARTStreamingEnabled": "No", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "640 Shutter-Description": "NI DAC", "528-Description": "DA device that is used as a shutter", "COM4-Description": "Serial port driver (boost:asio)", "405 Shutter-Description": "NI DAC", "TE2000-Name": "TE2000", "528 Shutter-Description": "NI DAC", "COM9-Description": "Serial port driver (boost:asio)", "LudlController-Name": "LudlController", "445 Shutter-TriggerSequenceLength": "1000", "PRIME-SerialNumber": "A18B203008", "445 AO-Volts": "0.0000", "PRIME-X-dimension": "1200", "PRIME-ShutterCloseDelay": "0", "XLIGHT Disk slider-State": "2", "Position": "Default", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 AO-IOChannel": "Dev1/ao5", "520 AO-IOChannel": "Dev1/ao4", "528 AO-TriggerSequenceLength": "1000", "405 Shutter-MaxVolts": "5.0000", "470-DA Device": "470 Shutter", "PRIME-FTCapable": "No", "Core-ChannelGroup": "Channels", "555 AO-SupportsTriggering": "0", "PRIME-Y-dimension": "1200", "Frame": 0, "555 Shutter-Sequenceable": "1", "PRIME-Timing-ExposureTimeNs": "499998000.0000", "Core-ImageProcessor": "", "528-State": "0", "520 AO-Block voltage": "false", "XLIGHT Excitation Wheel-Label": "QUAD", "640 Shutter-Demo": "No", "COM3-StopBits": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "520 Shutter-IOChannel": "Dev1/ao11", "Width": 1200, "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-SupportsTriggering": "0", "640 AO-TriggerSampleRate": "100000", "520-Description": "DA device that is used as a shutter", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "405 Shutter-Sequenceable": "1", "405 Shutter-SupportsTriggering": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-TriggerSampleRate": "100000", "640 Shutter-MaxVolts": "5.0000", "555 AO-MaxVolts": "5.0000", "520 AO-TriggerSequenceLength": "1000", "COM3-BaudRate": "9600", "COM9-BaudRate": "9600", "Vincent-D3-Channel #": "Ch. #1", "PRIME-PMode": "Normal", "OpticalPath-Label": "Left(100)", "BitDepth": 16, "555 AO-TriggerSequenceLength": "1000", "445 Shutter-Description": "NI DAC", "405 AO-Sequenceable": "1", "PRIME-AcquisitionMethod": "Callbacks", "555 AO-Name": "AnalogIO", "PRIME-ShutterMode": "Pre-Exposure", "COM3-Handshaking": "Off", "445 AO-SupportsTriggering": "0", "ROI": "0-0-1200-1200", "COM9-Verbose": "1", "PRIME-Color": "OFF", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "PRIME-ShutterOpenDelay": "0", "COM1-Parity": "None", "Slice": 7, "SlicePosition": 20, "COM9-DelayBetweenCharsMs": "0.0000", "555-Name": "DA Shutter", "PIEZO-LudlSingleAxisName": "B", "XLIGHT Emission Wheel-Label": "QUAD", "640 Shutter-Name": "AnalogIO", "640 AO-Sequenceable": "1", "520 Shutter-Demo": "No", "528 Shutter-Demo": "No", "470-Name": "DA Shutter", "520-DA Device": "520 Shutter", "XYStage-Description": "Ludl XY stage driver adapter", "445 Shutter-MinVolts": "0.0000", "528 AO-TriggerSampleRate": "100000", "405 AO-MinVolts": "0.0000", "405 Shutter-TriggerSequenceLength": "1000", "Height": 1200, "555 AO-Block voltage": "false", "640 Shutter-Volts": "0.0000", "PixelType": "GRAY16", "445 Shutter-Block voltage": "false", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "520 Shutter-TriggerSequenceLength": "1000", "COM9-Name": "COM9", "Core-SLM": "", "555 AO-Volts": "0.0000", "555 Shutter-Block voltage": "false", "XYStage-Speed": "1800.0000", "520 AO-MaxVolts": "5.0000", "528 Shutter-SupportsTriggering": "0", "COM1-Description": "Serial port driver (boost:asio)", "PRIME-MetadataEnabled": "No", "520 Shutter-MinVolts": "0.0000", "470 Shutter-Sequenceable": "1", "PRIME-PreampDelay": "0", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-MinVolts": "0.0000", "ChannelIndex": 5, "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Core-AutoShutter": "1", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "Exposure-ms": 300, "PRIME-PP 2 ENABLED": "Yes", "640 Shutter-TriggerSampleRate": "100000", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "470 Shutter-IOChannel": "Dev1/ao10", "405 AO-Demo": "No", "PixelSizeUm": 1.1, "470 AO-Demo": "No", "470 Shutter-MaxVolts": "5.0000", "Vincent-D3-Name": "Vincent-D3", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FileName": "img_000000000_P-DAPI-high_007.tif", "LudlController-Reset": "Operate", "PRIME-Exposure": "300.00", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "445-Name": "DA Shutter", "555 Shutter-Name": "AnalogIO", "520 Shutter-TriggerSampleRate": "100000", "445 Shutter-IOChannel": "Dev1/ao9", "PRIME-TransposeCorrection": "0", "XYStage-StepSize": "0.1000", "445-Description": "DA device that is used as a shutter", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 AO-IOChannel": "Dev1/ao7", "520 Shutter-Name": "AnalogIO", "COM3-DataBits": "8", "640 AO-Volts": "0.0000", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "555 Shutter-IOChannel": "Dev1/ao13", "WaitInterval": null, "520 Shutter-Sequenceable": "1", "YPositionUm": 31506.2, "445-State": "0", "445-DA Device": "445 Shutter", "PRIME-CircularBufferAutoSize": "ON", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "555 AO-TriggerSampleRate": "100000", "445 Shutter-Volts": "0.0000", "XLIGHT Hub-Port": "COM3", "Core-AutoFocus": "", "COM4-Handshaking": "Off", "528 Shutter-MinVolts": "0.0000", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "445 Shutter-Name": "AnalogIO", "528 Shutter-Sequenceable": "1", "XYStage-TransposeMirrorX": "0", "XYStage-TransposeMirrorY": "0", "PRIME-ReadoutRate": "100MHz 16bit", "XYStage-Name": "Stage", "640 AO-SupportsTriggering": "0", "640-Name": "DA Shutter", "640 Shutter-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "640 AO-Block voltage": "false", "445 Shutter-Sequenceable": "1", "470 AO-MaxVolts": "5.0000", "COM4-BaudRate": "9600", "470-Description": "DA device that is used as a shutter", "520 Shutter-Description": "NI DAC", "PRIME-CCDTemperature": "-14.8500", "Time": "2019-12-18 21:37:18 -0500", "640 AO-TriggerSequenceLength": "1000", "470 AO-Name": "AnalogIO", "XLIGHT Disk slider-Label": "Disk Pos 40um", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "Binning": "1x1", "TE2000-ModelType": "1", "COM3-AnswerTimeout": "500.0000", "555 Shutter-SupportsTriggering": "0", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "TE2000-Port": "COM4", "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Description": "NI DAC", "COM4-AnswerTimeout": "500.0000", "COM1-DelayBetweenCharsMs": "0.0000", "FilterBlock-Label": "Confocal", "445 AO-Name": "AnalogIO", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-Timing-ReadoutTimeNs": "24672000.0000", "528 AO-Demo": "No", "520 AO-Sequenceable": "1", "555-Description": "DA device that is used as a shutter", "405-DA Device": "405 Shutter", "405 AO-TriggerSequenceLength": "1000", "640 AO-MinVolts": "0.0000", "640 Shutter-Block voltage": "false", "FrameIndex": 0, "PRIME-Port": "CMOS", "555 AO-MinVolts": "0.0000", "COM3-Description": "Serial port driver (boost:asio)", "528 AO-Description": "NI DAC", "555 AO-IOChannel": "Dev1/ao6", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "555 AO-TriggerInputLine": "/Dev1/PFI0", "PRIME-ActualInterval-ms": "390.5200", "LudlController-Port": "COM9", "445 Shutter-Demo": "No", "PRIME-Trigger-Expose Out-Mux": "1", "SliceIndex": 7, "PRIME-CCDTemperatureSetPoint": "-15.0000", "405 Shutter-MinVolts": "0.0000", "PRIME-ExposeOutMode": "First Row", "AxisPositions": null, "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Volts": "0.0000", "528-Name": "DA Shutter", "555-State": "0", "XLIGHT Dichroic-Label": "QUAD", "445 AO-Sequenceable": "1", "405-Name": "DA Shutter", "405 AO-IOChannel": "Dev1/ao0", "Core-Camera": "PRIME", "Focus-Name": "Focus", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "405 Shutter-IOChannel": "Dev1/ao8", "640 AO-Demo": "No", "640 Shutter-SupportsTriggering": "0", "445 AO-Demo": "No", "555-DA Device": "555 Shutter", "470 Shutter-Description": "NI DAC", "528 AO-Volts": "0.0000", "COM1-DataBits": "8", "Vincent-D3-Address": "x", "520 AO-SupportsTriggering": "0", "405-Description": "DA device that is used as a shutter", "640 Shutter-MinVolts": "0.0000", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "405 AO-Name": "AnalogIO", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "640 AO-TriggerInputLine": "/Dev1/PFI0", "405 AO-TriggerInputLine": "/Dev1/PFI0", "640-Description": "DA device that is used as a shutter", "445 Shutter-TriggerSampleRate": "100000", "470 Shutter-TriggerSequenceLength": "1000", "COM1-StopBits": "1", "520 Shutter-SupportsTriggering": "0", "405-State": "1", "Core-Initialize": "1", "XLIGHT Emission Wheel-State": "3", "520 AO-Description": "NI DAC", "528 AO-TriggerInputLine": "/Dev1/PFI0", "445 Shutter-MaxVolts": "5.0000", "COM9-Handshaking": "Off", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "XLIGHT Spin motor-Label": "On (spinning)", "PRIME-PP 5 ENABLED": "No", "UUID": "3394bb1d-18ea-44ef-8abe-69da6ff42be5", "PRIME-TriggerMode": "Internal Trigger", "COM1-Handshaking": "Off", "PRIME-Timing-PostTriggerDelayNs": "49344000.0000", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "Focus-Position": "17741.5000", "528 Shutter-Block voltage": "false", "Channel": "P-DAPI-high", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "555 Shutter-Demo": "No", "405 Shutter-Demo": "No", "528 AO-Name": "AnalogIO", "520-State": "0", "Core-Galvo": "", "405 Shutter-TriggerSampleRate": "100000", "Focus-FocusStepSize_(nm)": "100", "PRIME-PixelType": "16bit", "COM4-DataBits": "8", "470 AO-IOChannel": "Dev1/ao2", "520 AO-MinVolts": "0.0000", "COM9-Parity": "None", "PRIME-CircularBufferEnabled": "ON", "PRIME-PP 6 Reset": "No", "640 AO-Description": "NI DAC", "Source": "PRIME", "445 AO-MinVolts": "0.0000", "PIEZO-Name": "Stage", "445 AO-Description": "NI DAC", "PRIME-CircularBufferFrameCount": "46", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "520 AO-Volts": "0.0000", "528 Shutter-TriggerSampleRate": "100000", "XPositionUm": 19731.9, "OpticalPath-State": "4", "PRIME-PP 3 THRESHOLD": "80", "PRIME-PVCAM Version": "3.7.1", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Gain": "1-HDR", "640 Shutter-TriggerSequenceLength": "1000", "528 AO-SupportsTriggering": "0", "445 AO-TriggerInputLine": "/Dev1/PFI0", "OpticalPath-Name": "OpticalPath", "PRIME-PP 4 ENABLED": "Yes", "PRIME-PVCAM Adapter Version": "1.3.29", "COM3-Name": "COM3", "405 AO-MaxVolts": "5.0000", "520 Shutter-Block voltage": "false", "PRIME-Timing-ClearingTimeNs": "49344000.0000", "Core-Focus": "PIEZO", "528 AO-Block voltage": "false", "TE2000-Description": "Nikon TE2000 hub", "528 AO-MinVolts": "0.0000", "COM4-Parity": "None", "Summary": { "Slices": 11, "ChContrastMin": [ 0, 0, 0, 0, 0, 0 ], "PixelAspect": 1, "SlicesFirst": true, "Interval_ms": 0, "Time": "2019-12-18 21:36:42 -0500", "Prefix": "seq_4", "InitialPositionList": [ { "Label": "Pos0", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.21], "Focus": [17741.5], "XYStage": [ 44542.200000000004, 31636.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos1", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.22], "Focus": [17741.4], "XYStage": [ 43801.100000000006, 31637.9 ] }, "GridColumnIndex": 0 }, { "Label": "Pos2", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.5], "XYStage": [ 38561.3, 32040.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos3", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.185], "Focus": [17741.3], "XYStage": [ 37698.4, 32041.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos4", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.16], "Focus": [17741.5], "XYStage": [ 32375.4, 31758.9 ] }, "GridColumnIndex": 0 }, { "Label": "Pos5", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.17000000000002], "Focus": [17741.3], "XYStage": [ 31770.7, 31758.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos6", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.4], "XYStage": [ 26536.800000000003, 31068.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos7", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.205], "Focus": [17741.4], "XYStage": [ 25420.2, 31068.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos8", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.85], "Focus": [17741.3], "XYStage": [ 21059, 31508 ] }, "GridColumnIndex": 0 }, { "Label": "Pos9", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.82], "Focus": [17741.3], "XYStage": [ 19731.9, 31506.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos10", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.79], "Focus": [17741.4], "XYStage": [ 14528.5, 32007.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos11", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.83], "Focus": [17741.2], "XYStage": [ 13830.300000000001, 32009.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos12", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.79], "Focus": [17741.3], "XYStage": [ 8775.300000000001, 32017.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos13", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.815], "Focus": [17741.3], "XYStage": [ 8109.200000000001, 32008.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos14", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.805], "Focus": [17741.3], "XYStage": [ 3483.3, 31908.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos15", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.82], "Focus": [17741.2], "XYStage": [ 2686.8, 31903.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos16", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [211.48000000000002], "Focus": [17741.1], "XYStage": [ -1978.9, 31973.600000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos17", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [211.51500000000001], "Focus": [17741.1], "XYStage": [ -2957.8, 31982.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos18", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.195], "Focus": [17741.5], "XYStage": [ 47747.4, -576.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos19", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.23000000000002], "Focus": [17741.6], "XYStage": [ 47053.9, -576.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos20", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.205], "Focus": [17741.4], "XYStage": [ 41871.200000000004, -479.8 ] }, "GridColumnIndex": 0 }, { "Label": "Pos21", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.4], "XYStage": [ 40882.600000000006, -392 ] }, "GridColumnIndex": 0 }, { "Label": "Pos22", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.24], "Focus": [17741.4], "XYStage": [ 35905.6, -135.3 ] }, "GridColumnIndex": 0 }, { "Label": "Pos23", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.195], "Focus": [17741.5], "XYStage": [ 34990.9, -132.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos24", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.46], "Focus": [17741.4], "XYStage": [ 29678.100000000002, -958.1 ] }, "GridColumnIndex": 0 }, { "Label": "Pos25", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.44], "Focus": [17741.5], "XYStage": [ 28983.600000000002, -939.4000000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos26", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.475], "Focus": [17741.5], "XYStage": [ 23019.7, -122 ] }, "GridColumnIndex": 0 }, { "Label": "Pos27", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.44], "Focus": [17741.7], "XYStage": [ 22428, -116.80000000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos28", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.46], "Focus": [17741.7], "XYStage": [ 16364.900000000001, -181.10000000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos29", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.48000000000002], "Focus": [17741.5], "XYStage": [ 15712.1, -180 ] }, "GridColumnIndex": 0 }, { "Label": "Pos30", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.45000000000002], "Focus": [17741.7], "XYStage": [ 10346.1, 77.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos31", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.49], "Focus": [17741.4], "XYStage": [ 9755.5, 85.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos32", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.475], "Focus": [17741.5], "XYStage": [ 4775.5, 293.90000000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos33", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.445], "Focus": [17741.4], "XYStage": [ 3954.9, 293.90000000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos34", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.455], "Focus": [17741.4], "XYStage": [ -1324.9, 545.6 ] }, "GridColumnIndex": 0 }, { "Label": "Pos35", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.445], "Focus": [17741.3], "XYStage": [ -2097, 576.6 ] }, "GridColumnIndex": 0 } ], "z-step_um": 10, "GridRow": 0, "BitDepth": 16, "Depth": 2, "Channels": 6, "Source": "Micro-Manager", "ChColors": [ -16711681, -256, -65281, -1, -1, -16776961 ], "MetadataVersion": 10, "KeepShutterOpenChannels": true, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536, 65536 ], "CustomIntervals_ms": [], "Height": 1200, "MicroManagerVersion": "1.4.23 20180219", "GridColumn": 0, "UUID": "14a13bf7-c944-4672-b873-fcfbbb7ea13d", "PixelType": "GRAY16", "Width": 1200, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\Yan\\20191218-YC113-S7S8-miseq", "Frames": 1, "Comment": "", "UserName": "Nikon", "PixelSize_um": 1.1, "ROI": [ 0, 0, 1200, 1200 ], "Date": "2019-12-18", "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC", "P-DAPI-high" ], "KeepShutterOpenSlices": true, "IJType": 1, "ComputerName": "ZadorConfocal2", "Positions": 36, "PositionIndex": 9, "CameraTimeout": 20000, "TimeFirst": false }, "640 AO-Name": "AnalogIO", "Vincent-D3-Command": "Close" }, "FrameKey-0-5-8": { "COM4-StopBits": "1", "528 AO-MaxVolts": "5.0000", "XLIGHT Excitation Wheel-State": "1", "470 Shutter-Name": "AnalogIO", "Core-Shutter": "405", "555 AO-Demo": "No", "XLIGHT Dichroic-State": "2", "XYStage-StepSize-Y": "0.1000", "ZPositionUm": 278.82, "XYStage-StepSize-X": "0.1000", "PRIME-FirmwareVersion": "20.28", "PRIME-ClearCycles": "2", "520-Name": "DA Shutter", "405 Shutter-Name": "AnalogIO", "PIEZO-StepSize": "0.0050", "PRIME-ClearMode": "Pre-Exposure", "470 AO-MinVolts": "0.0000", "405 AO-SupportsTriggering": "1", "CameraChannelIndex": 0, "XYStage-StartSpeed": "500.0000", "COM9-StopBits": "2", "405 AO-Description": "NI DAC", "COM4-DelayBetweenCharsMs": "0.0000", "445 AO-Block voltage": "false", "520 AO-TriggerInputLine": "/Dev1/PFI0", "555 AO-Description": "NI DAC", "520 AO-TriggerSampleRate": "100000", "COM4-Verbose": "1", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM4-Name": "COM4", "405 Shutter-Volts": "5.0000", "Vincent-D3-Port": "COM1", "PRIME-PP 4 MIN ADU AFFECTED": "200", "470 Shutter-MinVolts": "0.0000", "PRIME-PP 3 ENABLED": "Yes", "470 AO-Description": "NI DAC", "PRIME-Description": "PVCAM API device adapter", "520 Shutter-MaxVolts": "5.0000", "520 Shutter-Volts": "0.0000", "528 AO-Sequenceable": "1", "PRIME-Binning": "1x1", "555 Shutter-MaxVolts": "5.0000", "528 Shutter-TriggerSequenceLength": "1000", "405 Shutter-Block voltage": "false", "XLIGHT Spin motor-State": "1", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "Vincent-D3-Time to open (ms)": "35.0000", "Vincent-D3-Time to close (ms)": "35.0000", "445 AO-TriggerSampleRate": "100000", "640 Shutter-IOChannel": "Dev1/ao14", "555 AO-Sequenceable": "1", "520 AO-Name": "AnalogIO", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "470 AO-Block voltage": "false", "COM1-Verbose": "1", "COM9-AnswerTimeout": "10000.0000", "528 Shutter-Name": "AnalogIO", "PRIME-Trigger Timeout (secs)": "10", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "NextFrame": 0, "470 AO-TriggerSequenceLength": "1000", "PRIME-TransposeXY": "0", "445 AO-TriggerSequenceLength": "1000", "COM1-Name": "COM1", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "COM9-DataBits": "8", "COM1-AnswerTimeout": "500.0000", "Camera": "", "TE2000-Version": "V2.50-V2.50-V2.10", "528 Shutter-Volts": "0.0000", "XLIGHT Hub-Name": "XLIGHT Hub", "PIEZO-Description": "Ludl stage driver adapter", "PositionName": "Pos9", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSampleRate": "100000", "PRIME-FanSpeedSetpoint": "High", "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "405 AO-TriggerSampleRate": "100000", "470 Shutter-Block voltage": "false", "470-State": "0", "COM3-Parity": "None", "405 AO-Volts": "4.5000", "PRIME-TransposeMirrorY": "0", "PRIME-ChipName": "GS144BSI", "470 AO-Volts": "0.0000", "PRIME-TransposeMirrorX": "0", "445 Shutter-SupportsTriggering": "0", "470 Shutter-SupportsTriggering": "0", "XLIGHT Hub-Description": "XLIGHT hub", "PRIME-PreampOffLimit": "10000", "470 AO-Sequenceable": "1", "528 Shutter-MaxVolts": "5.0000", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "Core-XYStage": "XYStage", "470 AO-TriggerSampleRate": "100000", "PRIME-Timing-PreTriggerDelayNs": "24672000.0000", "528-DA Device": "528 Shutter", "Focus-Description": "Nikon TE2000 focus adapter", "FilterBlock-State": "5", "520 AO-Demo": "No", "COM3-DelayBetweenCharsMs": "0.0000", "470 Shutter-Volts": "0.0000", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "PRIME-PP 2 THRESHOLD": "125", "PRIME-PP 4 THRESHOLD": "75", "XYStage-Acceleration": "4.0000", "470 Shutter-Demo": "No", "PRIME-Name": "PMPCIECam00", "640-DA Device": "640 Shutter", "405 AO-Block voltage": "false", "ElapsedTime-ms": 411450, "PRIME-Offset": "100", "445 AO-IOChannel": "Dev1/ao1", "640 AO-MaxVolts": "5.0000", "PositionIndex": 9, "PRIME-PP 1 ENABLED": "Yes", "COM1-BaudRate": "9600", "640-State": "0", "COM3-Verbose": "1", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "LudlController-TransmissionDelay": "0", "PRIME-FullWellCapacity": "62000", "PRIME-SMARTStreamingEnabled": "No", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "640 Shutter-Description": "NI DAC", "528-Description": "DA device that is used as a shutter", "COM4-Description": "Serial port driver (boost:asio)", "405 Shutter-Description": "NI DAC", "TE2000-Name": "TE2000", "528 Shutter-Description": "NI DAC", "COM9-Description": "Serial port driver (boost:asio)", "LudlController-Name": "LudlController", "445 Shutter-TriggerSequenceLength": "1000", "PRIME-SerialNumber": "A18B203008", "445 AO-Volts": "0.0000", "PRIME-X-dimension": "1200", "PRIME-ShutterCloseDelay": "0", "XLIGHT Disk slider-State": "2", "Position": "Default", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 AO-IOChannel": "Dev1/ao5", "520 AO-IOChannel": "Dev1/ao4", "528 AO-TriggerSequenceLength": "1000", "405 Shutter-MaxVolts": "5.0000", "470-DA Device": "470 Shutter", "PRIME-FTCapable": "No", "Core-ChannelGroup": "Channels", "555 AO-SupportsTriggering": "0", "PRIME-Y-dimension": "1200", "Frame": 0, "555 Shutter-Sequenceable": "1", "PRIME-Timing-ExposureTimeNs": "499998000.0000", "Core-ImageProcessor": "", "528-State": "0", "520 AO-Block voltage": "false", "XLIGHT Excitation Wheel-Label": "QUAD", "640 Shutter-Demo": "No", "COM3-StopBits": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "520 Shutter-IOChannel": "Dev1/ao11", "Width": 1200, "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-SupportsTriggering": "0", "640 AO-TriggerSampleRate": "100000", "520-Description": "DA device that is used as a shutter", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "405 Shutter-Sequenceable": "1", "405 Shutter-SupportsTriggering": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-TriggerSampleRate": "100000", "640 Shutter-MaxVolts": "5.0000", "555 AO-MaxVolts": "5.0000", "520 AO-TriggerSequenceLength": "1000", "COM3-BaudRate": "9600", "COM9-BaudRate": "9600", "Vincent-D3-Channel #": "Ch. #1", "PRIME-PMode": "Normal", "OpticalPath-Label": "Left(100)", "BitDepth": 16, "555 AO-TriggerSequenceLength": "1000", "445 Shutter-Description": "NI DAC", "405 AO-Sequenceable": "1", "PRIME-AcquisitionMethod": "Callbacks", "555 AO-Name": "AnalogIO", "PRIME-ShutterMode": "Pre-Exposure", "COM3-Handshaking": "Off", "445 AO-SupportsTriggering": "0", "ROI": "0-0-1200-1200", "COM9-Verbose": "1", "PRIME-Color": "OFF", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "PRIME-ShutterOpenDelay": "0", "COM1-Parity": "None", "Slice": 8, "SlicePosition": 30, "COM9-DelayBetweenCharsMs": "0.0000", "555-Name": "DA Shutter", "PIEZO-LudlSingleAxisName": "B", "XLIGHT Emission Wheel-Label": "QUAD", "640 Shutter-Name": "AnalogIO", "640 AO-Sequenceable": "1", "520 Shutter-Demo": "No", "528 Shutter-Demo": "No", "470-Name": "DA Shutter", "520-DA Device": "520 Shutter", "XYStage-Description": "Ludl XY stage driver adapter", "445 Shutter-MinVolts": "0.0000", "528 AO-TriggerSampleRate": "100000", "405 AO-MinVolts": "0.0000", "405 Shutter-TriggerSequenceLength": "1000", "Height": 1200, "555 AO-Block voltage": "false", "640 Shutter-Volts": "0.0000", "PixelType": "GRAY16", "445 Shutter-Block voltage": "false", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "520 Shutter-TriggerSequenceLength": "1000", "COM9-Name": "COM9", "Core-SLM": "", "555 AO-Volts": "0.0000", "555 Shutter-Block voltage": "false", "XYStage-Speed": "1800.0000", "520 AO-MaxVolts": "5.0000", "528 Shutter-SupportsTriggering": "0", "COM1-Description": "Serial port driver (boost:asio)", "PRIME-MetadataEnabled": "No", "520 Shutter-MinVolts": "0.0000", "470 Shutter-Sequenceable": "1", "PRIME-PreampDelay": "0", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-MinVolts": "0.0000", "ChannelIndex": 5, "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Core-AutoShutter": "1", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "Exposure-ms": 300, "PRIME-PP 2 ENABLED": "Yes", "640 Shutter-TriggerSampleRate": "100000", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "470 Shutter-IOChannel": "Dev1/ao10", "405 AO-Demo": "No", "PixelSizeUm": 1.1, "470 AO-Demo": "No", "470 Shutter-MaxVolts": "5.0000", "Vincent-D3-Name": "Vincent-D3", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FileName": "img_000000000_P-DAPI-high_008.tif", "LudlController-Reset": "Operate", "PRIME-Exposure": "300.00", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "445-Name": "DA Shutter", "555 Shutter-Name": "AnalogIO", "520 Shutter-TriggerSampleRate": "100000", "445 Shutter-IOChannel": "Dev1/ao9", "PRIME-TransposeCorrection": "0", "XYStage-StepSize": "0.1000", "445-Description": "DA device that is used as a shutter", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 AO-IOChannel": "Dev1/ao7", "520 Shutter-Name": "AnalogIO", "COM3-DataBits": "8", "640 AO-Volts": "0.0000", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "555 Shutter-IOChannel": "Dev1/ao13", "WaitInterval": null, "520 Shutter-Sequenceable": "1", "YPositionUm": 31506.2, "445-State": "0", "445-DA Device": "445 Shutter", "PRIME-CircularBufferAutoSize": "ON", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "555 AO-TriggerSampleRate": "100000", "445 Shutter-Volts": "0.0000", "XLIGHT Hub-Port": "COM3", "Core-AutoFocus": "", "COM4-Handshaking": "Off", "528 Shutter-MinVolts": "0.0000", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "445 Shutter-Name": "AnalogIO", "528 Shutter-Sequenceable": "1", "XYStage-TransposeMirrorX": "0", "XYStage-TransposeMirrorY": "0", "PRIME-ReadoutRate": "100MHz 16bit", "XYStage-Name": "Stage", "640 AO-SupportsTriggering": "0", "640-Name": "DA Shutter", "640 Shutter-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "640 AO-Block voltage": "false", "445 Shutter-Sequenceable": "1", "470 AO-MaxVolts": "5.0000", "COM4-BaudRate": "9600", "470-Description": "DA device that is used as a shutter", "520 Shutter-Description": "NI DAC", "PRIME-CCDTemperature": "-14.8500", "Time": "2019-12-18 21:37:19 -0500", "640 AO-TriggerSequenceLength": "1000", "470 AO-Name": "AnalogIO", "XLIGHT Disk slider-Label": "Disk Pos 40um", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "Binning": "1x1", "TE2000-ModelType": "1", "COM3-AnswerTimeout": "500.0000", "555 Shutter-SupportsTriggering": "0", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "TE2000-Port": "COM4", "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Description": "NI DAC", "COM4-AnswerTimeout": "500.0000", "COM1-DelayBetweenCharsMs": "0.0000", "FilterBlock-Label": "Confocal", "445 AO-Name": "AnalogIO", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-Timing-ReadoutTimeNs": "24672000.0000", "528 AO-Demo": "No", "520 AO-Sequenceable": "1", "555-Description": "DA device that is used as a shutter", "405-DA Device": "405 Shutter", "405 AO-TriggerSequenceLength": "1000", "640 AO-MinVolts": "0.0000", "640 Shutter-Block voltage": "false", "FrameIndex": 0, "PRIME-Port": "CMOS", "555 AO-MinVolts": "0.0000", "COM3-Description": "Serial port driver (boost:asio)", "528 AO-Description": "NI DAC", "555 AO-IOChannel": "Dev1/ao6", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "555 AO-TriggerInputLine": "/Dev1/PFI0", "PRIME-ActualInterval-ms": "390.5200", "LudlController-Port": "COM9", "445 Shutter-Demo": "No", "PRIME-Trigger-Expose Out-Mux": "1", "SliceIndex": 8, "PRIME-CCDTemperatureSetPoint": "-15.0000", "405 Shutter-MinVolts": "0.0000", "PRIME-ExposeOutMode": "First Row", "AxisPositions": null, "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Volts": "0.0000", "528-Name": "DA Shutter", "555-State": "0", "XLIGHT Dichroic-Label": "QUAD", "445 AO-Sequenceable": "1", "405-Name": "DA Shutter", "405 AO-IOChannel": "Dev1/ao0", "Core-Camera": "PRIME", "Focus-Name": "Focus", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "405 Shutter-IOChannel": "Dev1/ao8", "640 AO-Demo": "No", "640 Shutter-SupportsTriggering": "0", "445 AO-Demo": "No", "555-DA Device": "555 Shutter", "470 Shutter-Description": "NI DAC", "528 AO-Volts": "0.0000", "COM1-DataBits": "8", "Vincent-D3-Address": "x", "520 AO-SupportsTriggering": "0", "405-Description": "DA device that is used as a shutter", "640 Shutter-MinVolts": "0.0000", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "405 AO-Name": "AnalogIO", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "640 AO-TriggerInputLine": "/Dev1/PFI0", "405 AO-TriggerInputLine": "/Dev1/PFI0", "640-Description": "DA device that is used as a shutter", "445 Shutter-TriggerSampleRate": "100000", "470 Shutter-TriggerSequenceLength": "1000", "COM1-StopBits": "1", "520 Shutter-SupportsTriggering": "0", "405-State": "1", "Core-Initialize": "1", "XLIGHT Emission Wheel-State": "3", "520 AO-Description": "NI DAC", "528 AO-TriggerInputLine": "/Dev1/PFI0", "445 Shutter-MaxVolts": "5.0000", "COM9-Handshaking": "Off", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "XLIGHT Spin motor-Label": "On (spinning)", "PRIME-PP 5 ENABLED": "No", "UUID": "9bf05022-436d-467e-bb5e-cc065c440ef9", "PRIME-TriggerMode": "Internal Trigger", "COM1-Handshaking": "Off", "PRIME-Timing-PostTriggerDelayNs": "49344000.0000", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "Focus-Position": "17741.5000", "528 Shutter-Block voltage": "false", "Channel": "P-DAPI-high", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "555 Shutter-Demo": "No", "405 Shutter-Demo": "No", "528 AO-Name": "AnalogIO", "520-State": "0", "Core-Galvo": "", "405 Shutter-TriggerSampleRate": "100000", "Focus-FocusStepSize_(nm)": "100", "PRIME-PixelType": "16bit", "COM4-DataBits": "8", "470 AO-IOChannel": "Dev1/ao2", "520 AO-MinVolts": "0.0000", "COM9-Parity": "None", "PRIME-CircularBufferEnabled": "ON", "PRIME-PP 6 Reset": "No", "640 AO-Description": "NI DAC", "Source": "PRIME", "445 AO-MinVolts": "0.0000", "PIEZO-Name": "Stage", "445 AO-Description": "NI DAC", "PRIME-CircularBufferFrameCount": "46", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "520 AO-Volts": "0.0000", "528 Shutter-TriggerSampleRate": "100000", "XPositionUm": 19731.9, "OpticalPath-State": "4", "PRIME-PP 3 THRESHOLD": "80", "PRIME-PVCAM Version": "3.7.1", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Gain": "1-HDR", "640 Shutter-TriggerSequenceLength": "1000", "528 AO-SupportsTriggering": "0", "445 AO-TriggerInputLine": "/Dev1/PFI0", "OpticalPath-Name": "OpticalPath", "PRIME-PP 4 ENABLED": "Yes", "PRIME-PVCAM Adapter Version": "1.3.29", "COM3-Name": "COM3", "405 AO-MaxVolts": "5.0000", "520 Shutter-Block voltage": "false", "PRIME-Timing-ClearingTimeNs": "49344000.0000", "Core-Focus": "PIEZO", "528 AO-Block voltage": "false", "TE2000-Description": "Nikon TE2000 hub", "528 AO-MinVolts": "0.0000", "COM4-Parity": "None", "Summary": { "Slices": 11, "ChContrastMin": [ 0, 0, 0, 0, 0, 0 ], "PixelAspect": 1, "SlicesFirst": true, "Interval_ms": 0, "Time": "2019-12-18 21:36:42 -0500", "Prefix": "seq_4", "InitialPositionList": [ { "Label": "Pos0", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.21], "Focus": [17741.5], "XYStage": [ 44542.200000000004, 31636.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos1", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.22], "Focus": [17741.4], "XYStage": [ 43801.100000000006, 31637.9 ] }, "GridColumnIndex": 0 }, { "Label": "Pos2", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.5], "XYStage": [ 38561.3, 32040.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos3", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.185], "Focus": [17741.3], "XYStage": [ 37698.4, 32041.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos4", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.16], "Focus": [17741.5], "XYStage": [ 32375.4, 31758.9 ] }, "GridColumnIndex": 0 }, { "Label": "Pos5", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.17000000000002], "Focus": [17741.3], "XYStage": [ 31770.7, 31758.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos6", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.4], "XYStage": [ 26536.800000000003, 31068.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos7", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.205], "Focus": [17741.4], "XYStage": [ 25420.2, 31068.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos8", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.85], "Focus": [17741.3], "XYStage": [ 21059, 31508 ] }, "GridColumnIndex": 0 }, { "Label": "Pos9", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.82], "Focus": [17741.3], "XYStage": [ 19731.9, 31506.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos10", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.79], "Focus": [17741.4], "XYStage": [ 14528.5, 32007.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos11", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.83], "Focus": [17741.2], "XYStage": [ 13830.300000000001, 32009.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos12", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.79], "Focus": [17741.3], "XYStage": [ 8775.300000000001, 32017.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos13", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.815], "Focus": [17741.3], "XYStage": [ 8109.200000000001, 32008.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos14", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.805], "Focus": [17741.3], "XYStage": [ 3483.3, 31908.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos15", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.82], "Focus": [17741.2], "XYStage": [ 2686.8, 31903.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos16", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [211.48000000000002], "Focus": [17741.1], "XYStage": [ -1978.9, 31973.600000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos17", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [211.51500000000001], "Focus": [17741.1], "XYStage": [ -2957.8, 31982.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos18", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.195], "Focus": [17741.5], "XYStage": [ 47747.4, -576.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos19", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.23000000000002], "Focus": [17741.6], "XYStage": [ 47053.9, -576.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos20", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.205], "Focus": [17741.4], "XYStage": [ 41871.200000000004, -479.8 ] }, "GridColumnIndex": 0 }, { "Label": "Pos21", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.4], "XYStage": [ 40882.600000000006, -392 ] }, "GridColumnIndex": 0 }, { "Label": "Pos22", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.24], "Focus": [17741.4], "XYStage": [ 35905.6, -135.3 ] }, "GridColumnIndex": 0 }, { "Label": "Pos23", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.195], "Focus": [17741.5], "XYStage": [ 34990.9, -132.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos24", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.46], "Focus": [17741.4], "XYStage": [ 29678.100000000002, -958.1 ] }, "GridColumnIndex": 0 }, { "Label": "Pos25", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.44], "Focus": [17741.5], "XYStage": [ 28983.600000000002, -939.4000000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos26", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.475], "Focus": [17741.5], "XYStage": [ 23019.7, -122 ] }, "GridColumnIndex": 0 }, { "Label": "Pos27", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.44], "Focus": [17741.7], "XYStage": [ 22428, -116.80000000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos28", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.46], "Focus": [17741.7], "XYStage": [ 16364.900000000001, -181.10000000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos29", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.48000000000002], "Focus": [17741.5], "XYStage": [ 15712.1, -180 ] }, "GridColumnIndex": 0 }, { "Label": "Pos30", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.45000000000002], "Focus": [17741.7], "XYStage": [ 10346.1, 77.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos31", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.49], "Focus": [17741.4], "XYStage": [ 9755.5, 85.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos32", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.475], "Focus": [17741.5], "XYStage": [ 4775.5, 293.90000000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos33", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.445], "Focus": [17741.4], "XYStage": [ 3954.9, 293.90000000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos34", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.455], "Focus": [17741.4], "XYStage": [ -1324.9, 545.6 ] }, "GridColumnIndex": 0 }, { "Label": "Pos35", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.445], "Focus": [17741.3], "XYStage": [ -2097, 576.6 ] }, "GridColumnIndex": 0 } ], "z-step_um": 10, "GridRow": 0, "BitDepth": 16, "Depth": 2, "Channels": 6, "Source": "Micro-Manager", "ChColors": [ -16711681, -256, -65281, -1, -1, -16776961 ], "MetadataVersion": 10, "KeepShutterOpenChannels": true, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536, 65536 ], "CustomIntervals_ms": [], "Height": 1200, "MicroManagerVersion": "1.4.23 20180219", "GridColumn": 0, "UUID": "14a13bf7-c944-4672-b873-fcfbbb7ea13d", "PixelType": "GRAY16", "Width": 1200, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\Yan\\20191218-YC113-S7S8-miseq", "Frames": 1, "Comment": "", "UserName": "Nikon", "PixelSize_um": 1.1, "ROI": [ 0, 0, 1200, 1200 ], "Date": "2019-12-18", "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC", "P-DAPI-high" ], "KeepShutterOpenSlices": true, "IJType": 1, "ComputerName": "ZadorConfocal2", "Positions": 36, "PositionIndex": 9, "CameraTimeout": 20000, "TimeFirst": false }, "640 AO-Name": "AnalogIO", "Vincent-D3-Command": "Close" }, "FrameKey-0-5-9": { "COM4-StopBits": "1", "528 AO-MaxVolts": "5.0000", "XLIGHT Excitation Wheel-State": "1", "470 Shutter-Name": "AnalogIO", "Core-Shutter": "405", "555 AO-Demo": "No", "XLIGHT Dichroic-State": "2", "XYStage-StepSize-Y": "0.1000", "ZPositionUm": 288.82, "XYStage-StepSize-X": "0.1000", "PRIME-FirmwareVersion": "20.28", "PRIME-ClearCycles": "2", "520-Name": "DA Shutter", "405 Shutter-Name": "AnalogIO", "PIEZO-StepSize": "0.0050", "PRIME-ClearMode": "Pre-Exposure", "470 AO-MinVolts": "0.0000", "405 AO-SupportsTriggering": "1", "CameraChannelIndex": 0, "XYStage-StartSpeed": "500.0000", "COM9-StopBits": "2", "405 AO-Description": "NI DAC", "COM4-DelayBetweenCharsMs": "0.0000", "445 AO-Block voltage": "false", "520 AO-TriggerInputLine": "/Dev1/PFI0", "555 AO-Description": "NI DAC", "520 AO-TriggerSampleRate": "100000", "COM4-Verbose": "1", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM4-Name": "COM4", "405 Shutter-Volts": "5.0000", "Vincent-D3-Port": "COM1", "PRIME-PP 4 MIN ADU AFFECTED": "200", "470 Shutter-MinVolts": "0.0000", "PRIME-PP 3 ENABLED": "Yes", "470 AO-Description": "NI DAC", "PRIME-Description": "PVCAM API device adapter", "520 Shutter-MaxVolts": "5.0000", "520 Shutter-Volts": "0.0000", "528 AO-Sequenceable": "1", "PRIME-Binning": "1x1", "555 Shutter-MaxVolts": "5.0000", "528 Shutter-TriggerSequenceLength": "1000", "405 Shutter-Block voltage": "false", "XLIGHT Spin motor-State": "1", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "Vincent-D3-Time to open (ms)": "35.0000", "Vincent-D3-Time to close (ms)": "35.0000", "445 AO-TriggerSampleRate": "100000", "640 Shutter-IOChannel": "Dev1/ao14", "555 AO-Sequenceable": "1", "520 AO-Name": "AnalogIO", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "470 AO-Block voltage": "false", "COM1-Verbose": "1", "COM9-AnswerTimeout": "10000.0000", "528 Shutter-Name": "AnalogIO", "PRIME-Trigger Timeout (secs)": "10", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "NextFrame": 0, "470 AO-TriggerSequenceLength": "1000", "PRIME-TransposeXY": "0", "445 AO-TriggerSequenceLength": "1000", "COM1-Name": "COM1", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "COM9-DataBits": "8", "COM1-AnswerTimeout": "500.0000", "Camera": "", "TE2000-Version": "V2.50-V2.50-V2.10", "528 Shutter-Volts": "0.0000", "XLIGHT Hub-Name": "XLIGHT Hub", "PIEZO-Description": "Ludl stage driver adapter", "PositionName": "Pos9", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSampleRate": "100000", "PRIME-FanSpeedSetpoint": "High", "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "405 AO-TriggerSampleRate": "100000", "470 Shutter-Block voltage": "false", "470-State": "0", "COM3-Parity": "None", "405 AO-Volts": "4.5000", "PRIME-TransposeMirrorY": "0", "PRIME-ChipName": "GS144BSI", "470 AO-Volts": "0.0000", "PRIME-TransposeMirrorX": "0", "445 Shutter-SupportsTriggering": "0", "470 Shutter-SupportsTriggering": "0", "XLIGHT Hub-Description": "XLIGHT hub", "PRIME-PreampOffLimit": "10000", "470 AO-Sequenceable": "1", "528 Shutter-MaxVolts": "5.0000", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "Core-XYStage": "XYStage", "470 AO-TriggerSampleRate": "100000", "PRIME-Timing-PreTriggerDelayNs": "24672000.0000", "528-DA Device": "528 Shutter", "Focus-Description": "Nikon TE2000 focus adapter", "FilterBlock-State": "5", "520 AO-Demo": "No", "COM3-DelayBetweenCharsMs": "0.0000", "470 Shutter-Volts": "0.0000", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "PRIME-PP 2 THRESHOLD": "125", "PRIME-PP 4 THRESHOLD": "75", "XYStage-Acceleration": "4.0000", "470 Shutter-Demo": "No", "PRIME-Name": "PMPCIECam00", "640-DA Device": "640 Shutter", "405 AO-Block voltage": "false", "ElapsedTime-ms": 412043, "PRIME-Offset": "100", "445 AO-IOChannel": "Dev1/ao1", "640 AO-MaxVolts": "5.0000", "PositionIndex": 9, "PRIME-PP 1 ENABLED": "Yes", "COM1-BaudRate": "9600", "640-State": "0", "COM3-Verbose": "1", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "LudlController-TransmissionDelay": "0", "PRIME-FullWellCapacity": "62000", "PRIME-SMARTStreamingEnabled": "No", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "640 Shutter-Description": "NI DAC", "528-Description": "DA device that is used as a shutter", "COM4-Description": "Serial port driver (boost:asio)", "405 Shutter-Description": "NI DAC", "TE2000-Name": "TE2000", "528 Shutter-Description": "NI DAC", "COM9-Description": "Serial port driver (boost:asio)", "LudlController-Name": "LudlController", "445 Shutter-TriggerSequenceLength": "1000", "PRIME-SerialNumber": "A18B203008", "445 AO-Volts": "0.0000", "PRIME-X-dimension": "1200", "PRIME-ShutterCloseDelay": "0", "XLIGHT Disk slider-State": "2", "Position": "Default", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 AO-IOChannel": "Dev1/ao5", "520 AO-IOChannel": "Dev1/ao4", "528 AO-TriggerSequenceLength": "1000", "405 Shutter-MaxVolts": "5.0000", "470-DA Device": "470 Shutter", "PRIME-FTCapable": "No", "Core-ChannelGroup": "Channels", "555 AO-SupportsTriggering": "0", "PRIME-Y-dimension": "1200", "Frame": 0, "555 Shutter-Sequenceable": "1", "PRIME-Timing-ExposureTimeNs": "499998000.0000", "Core-ImageProcessor": "", "528-State": "0", "520 AO-Block voltage": "false", "XLIGHT Excitation Wheel-Label": "QUAD", "640 Shutter-Demo": "No", "COM3-StopBits": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "520 Shutter-IOChannel": "Dev1/ao11", "Width": 1200, "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-SupportsTriggering": "0", "640 AO-TriggerSampleRate": "100000", "520-Description": "DA device that is used as a shutter", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "405 Shutter-Sequenceable": "1", "405 Shutter-SupportsTriggering": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-TriggerSampleRate": "100000", "640 Shutter-MaxVolts": "5.0000", "555 AO-MaxVolts": "5.0000", "520 AO-TriggerSequenceLength": "1000", "COM3-BaudRate": "9600", "COM9-BaudRate": "9600", "Vincent-D3-Channel #": "Ch. #1", "PRIME-PMode": "Normal", "OpticalPath-Label": "Left(100)", "BitDepth": 16, "555 AO-TriggerSequenceLength": "1000", "445 Shutter-Description": "NI DAC", "405 AO-Sequenceable": "1", "PRIME-AcquisitionMethod": "Callbacks", "555 AO-Name": "AnalogIO", "PRIME-ShutterMode": "Pre-Exposure", "COM3-Handshaking": "Off", "445 AO-SupportsTriggering": "0", "ROI": "0-0-1200-1200", "COM9-Verbose": "1", "PRIME-Color": "OFF", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "PRIME-ShutterOpenDelay": "0", "COM1-Parity": "None", "Slice": 9, "SlicePosition": 40, "COM9-DelayBetweenCharsMs": "0.0000", "555-Name": "DA Shutter", "PIEZO-LudlSingleAxisName": "B", "XLIGHT Emission Wheel-Label": "QUAD", "640 Shutter-Name": "AnalogIO", "640 AO-Sequenceable": "1", "520 Shutter-Demo": "No", "528 Shutter-Demo": "No", "470-Name": "DA Shutter", "520-DA Device": "520 Shutter", "XYStage-Description": "Ludl XY stage driver adapter", "445 Shutter-MinVolts": "0.0000", "528 AO-TriggerSampleRate": "100000", "405 AO-MinVolts": "0.0000", "405 Shutter-TriggerSequenceLength": "1000", "Height": 1200, "555 AO-Block voltage": "false", "640 Shutter-Volts": "0.0000", "PixelType": "GRAY16", "445 Shutter-Block voltage": "false", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "520 Shutter-TriggerSequenceLength": "1000", "COM9-Name": "COM9", "Core-SLM": "", "555 AO-Volts": "0.0000", "555 Shutter-Block voltage": "false", "XYStage-Speed": "1800.0000", "520 AO-MaxVolts": "5.0000", "528 Shutter-SupportsTriggering": "0", "COM1-Description": "Serial port driver (boost:asio)", "PRIME-MetadataEnabled": "No", "520 Shutter-MinVolts": "0.0000", "470 Shutter-Sequenceable": "1", "PRIME-PreampDelay": "0", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-MinVolts": "0.0000", "ChannelIndex": 5, "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Core-AutoShutter": "1", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "Exposure-ms": 300, "PRIME-PP 2 ENABLED": "Yes", "640 Shutter-TriggerSampleRate": "100000", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "470 Shutter-IOChannel": "Dev1/ao10", "405 AO-Demo": "No", "PixelSizeUm": 1.1, "470 AO-Demo": "No", "470 Shutter-MaxVolts": "5.0000", "Vincent-D3-Name": "Vincent-D3", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FileName": "img_000000000_P-DAPI-high_009.tif", "LudlController-Reset": "Operate", "PRIME-Exposure": "300.00", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "445-Name": "DA Shutter", "555 Shutter-Name": "AnalogIO", "520 Shutter-TriggerSampleRate": "100000", "445 Shutter-IOChannel": "Dev1/ao9", "PRIME-TransposeCorrection": "0", "XYStage-StepSize": "0.1000", "445-Description": "DA device that is used as a shutter", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 AO-IOChannel": "Dev1/ao7", "520 Shutter-Name": "AnalogIO", "COM3-DataBits": "8", "640 AO-Volts": "0.0000", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "555 Shutter-IOChannel": "Dev1/ao13", "WaitInterval": null, "520 Shutter-Sequenceable": "1", "YPositionUm": 31506.2, "445-State": "0", "445-DA Device": "445 Shutter", "PRIME-CircularBufferAutoSize": "ON", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "555 AO-TriggerSampleRate": "100000", "445 Shutter-Volts": "0.0000", "XLIGHT Hub-Port": "COM3", "Core-AutoFocus": "", "COM4-Handshaking": "Off", "528 Shutter-MinVolts": "0.0000", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "445 Shutter-Name": "AnalogIO", "528 Shutter-Sequenceable": "1", "XYStage-TransposeMirrorX": "0", "XYStage-TransposeMirrorY": "0", "PRIME-ReadoutRate": "100MHz 16bit", "XYStage-Name": "Stage", "640 AO-SupportsTriggering": "0", "640-Name": "DA Shutter", "640 Shutter-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "640 AO-Block voltage": "false", "445 Shutter-Sequenceable": "1", "470 AO-MaxVolts": "5.0000", "COM4-BaudRate": "9600", "470-Description": "DA device that is used as a shutter", "520 Shutter-Description": "NI DAC", "PRIME-CCDTemperature": "-14.8500", "Time": "2019-12-18 21:37:19 -0500", "640 AO-TriggerSequenceLength": "1000", "470 AO-Name": "AnalogIO", "XLIGHT Disk slider-Label": "Disk Pos 40um", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "Binning": "1x1", "TE2000-ModelType": "1", "COM3-AnswerTimeout": "500.0000", "555 Shutter-SupportsTriggering": "0", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "TE2000-Port": "COM4", "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Description": "NI DAC", "COM4-AnswerTimeout": "500.0000", "COM1-DelayBetweenCharsMs": "0.0000", "FilterBlock-Label": "Confocal", "445 AO-Name": "AnalogIO", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-Timing-ReadoutTimeNs": "24672000.0000", "528 AO-Demo": "No", "520 AO-Sequenceable": "1", "555-Description": "DA device that is used as a shutter", "405-DA Device": "405 Shutter", "405 AO-TriggerSequenceLength": "1000", "640 AO-MinVolts": "0.0000", "640 Shutter-Block voltage": "false", "FrameIndex": 0, "PRIME-Port": "CMOS", "555 AO-MinVolts": "0.0000", "COM3-Description": "Serial port driver (boost:asio)", "528 AO-Description": "NI DAC", "555 AO-IOChannel": "Dev1/ao6", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "555 AO-TriggerInputLine": "/Dev1/PFI0", "PRIME-ActualInterval-ms": "390.5200", "LudlController-Port": "COM9", "445 Shutter-Demo": "No", "PRIME-Trigger-Expose Out-Mux": "1", "SliceIndex": 9, "PRIME-CCDTemperatureSetPoint": "-15.0000", "405 Shutter-MinVolts": "0.0000", "PRIME-ExposeOutMode": "First Row", "AxisPositions": null, "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Volts": "0.0000", "528-Name": "DA Shutter", "555-State": "0", "XLIGHT Dichroic-Label": "QUAD", "445 AO-Sequenceable": "1", "405-Name": "DA Shutter", "405 AO-IOChannel": "Dev1/ao0", "Core-Camera": "PRIME", "Focus-Name": "Focus", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "405 Shutter-IOChannel": "Dev1/ao8", "640 AO-Demo": "No", "640 Shutter-SupportsTriggering": "0", "445 AO-Demo": "No", "555-DA Device": "555 Shutter", "470 Shutter-Description": "NI DAC", "528 AO-Volts": "0.0000", "COM1-DataBits": "8", "Vincent-D3-Address": "x", "520 AO-SupportsTriggering": "0", "405-Description": "DA device that is used as a shutter", "640 Shutter-MinVolts": "0.0000", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "405 AO-Name": "AnalogIO", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "640 AO-TriggerInputLine": "/Dev1/PFI0", "405 AO-TriggerInputLine": "/Dev1/PFI0", "640-Description": "DA device that is used as a shutter", "445 Shutter-TriggerSampleRate": "100000", "470 Shutter-TriggerSequenceLength": "1000", "COM1-StopBits": "1", "520 Shutter-SupportsTriggering": "0", "405-State": "1", "Core-Initialize": "1", "XLIGHT Emission Wheel-State": "3", "520 AO-Description": "NI DAC", "528 AO-TriggerInputLine": "/Dev1/PFI0", "445 Shutter-MaxVolts": "5.0000", "COM9-Handshaking": "Off", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "XLIGHT Spin motor-Label": "On (spinning)", "PRIME-PP 5 ENABLED": "No", "UUID": "9211b897-4df2-4360-a74f-599b560309ec", "PRIME-TriggerMode": "Internal Trigger", "COM1-Handshaking": "Off", "PRIME-Timing-PostTriggerDelayNs": "49344000.0000", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "Focus-Position": "17741.5000", "528 Shutter-Block voltage": "false", "Channel": "P-DAPI-high", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "555 Shutter-Demo": "No", "405 Shutter-Demo": "No", "528 AO-Name": "AnalogIO", "520-State": "0", "Core-Galvo": "", "405 Shutter-TriggerSampleRate": "100000", "Focus-FocusStepSize_(nm)": "100", "PRIME-PixelType": "16bit", "COM4-DataBits": "8", "470 AO-IOChannel": "Dev1/ao2", "520 AO-MinVolts": "0.0000", "COM9-Parity": "None", "PRIME-CircularBufferEnabled": "ON", "PRIME-PP 6 Reset": "No", "640 AO-Description": "NI DAC", "Source": "PRIME", "445 AO-MinVolts": "0.0000", "PIEZO-Name": "Stage", "445 AO-Description": "NI DAC", "PRIME-CircularBufferFrameCount": "46", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "520 AO-Volts": "0.0000", "528 Shutter-TriggerSampleRate": "100000", "XPositionUm": 19731.9, "OpticalPath-State": "4", "PRIME-PP 3 THRESHOLD": "80", "PRIME-PVCAM Version": "3.7.1", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Gain": "1-HDR", "640 Shutter-TriggerSequenceLength": "1000", "528 AO-SupportsTriggering": "0", "445 AO-TriggerInputLine": "/Dev1/PFI0", "OpticalPath-Name": "OpticalPath", "PRIME-PP 4 ENABLED": "Yes", "PRIME-PVCAM Adapter Version": "1.3.29", "COM3-Name": "COM3", "405 AO-MaxVolts": "5.0000", "520 Shutter-Block voltage": "false", "PRIME-Timing-ClearingTimeNs": "49344000.0000", "Core-Focus": "PIEZO", "528 AO-Block voltage": "false", "TE2000-Description": "Nikon TE2000 hub", "528 AO-MinVolts": "0.0000", "COM4-Parity": "None", "Summary": { "Slices": 11, "ChContrastMin": [ 0, 0, 0, 0, 0, 0 ], "PixelAspect": 1, "SlicesFirst": true, "Interval_ms": 0, "Time": "2019-12-18 21:36:42 -0500", "Prefix": "seq_4", "InitialPositionList": [ { "Label": "Pos0", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.21], "Focus": [17741.5], "XYStage": [ 44542.200000000004, 31636.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos1", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.22], "Focus": [17741.4], "XYStage": [ 43801.100000000006, 31637.9 ] }, "GridColumnIndex": 0 }, { "Label": "Pos2", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.5], "XYStage": [ 38561.3, 32040.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos3", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.185], "Focus": [17741.3], "XYStage": [ 37698.4, 32041.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos4", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.16], "Focus": [17741.5], "XYStage": [ 32375.4, 31758.9 ] }, "GridColumnIndex": 0 }, { "Label": "Pos5", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.17000000000002], "Focus": [17741.3], "XYStage": [ 31770.7, 31758.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos6", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.4], "XYStage": [ 26536.800000000003, 31068.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos7", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.205], "Focus": [17741.4], "XYStage": [ 25420.2, 31068.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos8", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.85], "Focus": [17741.3], "XYStage": [ 21059, 31508 ] }, "GridColumnIndex": 0 }, { "Label": "Pos9", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.82], "Focus": [17741.3], "XYStage": [ 19731.9, 31506.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos10", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.79], "Focus": [17741.4], "XYStage": [ 14528.5, 32007.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos11", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.83], "Focus": [17741.2], "XYStage": [ 13830.300000000001, 32009.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos12", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.79], "Focus": [17741.3], "XYStage": [ 8775.300000000001, 32017.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos13", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.815], "Focus": [17741.3], "XYStage": [ 8109.200000000001, 32008.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos14", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.805], "Focus": [17741.3], "XYStage": [ 3483.3, 31908.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos15", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.82], "Focus": [17741.2], "XYStage": [ 2686.8, 31903.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos16", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [211.48000000000002], "Focus": [17741.1], "XYStage": [ -1978.9, 31973.600000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos17", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [211.51500000000001], "Focus": [17741.1], "XYStage": [ -2957.8, 31982.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos18", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.195], "Focus": [17741.5], "XYStage": [ 47747.4, -576.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos19", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.23000000000002], "Focus": [17741.6], "XYStage": [ 47053.9, -576.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos20", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.205], "Focus": [17741.4], "XYStage": [ 41871.200000000004, -479.8 ] }, "GridColumnIndex": 0 }, { "Label": "Pos21", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.4], "XYStage": [ 40882.600000000006, -392 ] }, "GridColumnIndex": 0 }, { "Label": "Pos22", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.24], "Focus": [17741.4], "XYStage": [ 35905.6, -135.3 ] }, "GridColumnIndex": 0 }, { "Label": "Pos23", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.195], "Focus": [17741.5], "XYStage": [ 34990.9, -132.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos24", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.46], "Focus": [17741.4], "XYStage": [ 29678.100000000002, -958.1 ] }, "GridColumnIndex": 0 }, { "Label": "Pos25", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.44], "Focus": [17741.5], "XYStage": [ 28983.600000000002, -939.4000000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos26", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.475], "Focus": [17741.5], "XYStage": [ 23019.7, -122 ] }, "GridColumnIndex": 0 }, { "Label": "Pos27", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.44], "Focus": [17741.7], "XYStage": [ 22428, -116.80000000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos28", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.46], "Focus": [17741.7], "XYStage": [ 16364.900000000001, -181.10000000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos29", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.48000000000002], "Focus": [17741.5], "XYStage": [ 15712.1, -180 ] }, "GridColumnIndex": 0 }, { "Label": "Pos30", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.45000000000002], "Focus": [17741.7], "XYStage": [ 10346.1, 77.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos31", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.49], "Focus": [17741.4], "XYStage": [ 9755.5, 85.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos32", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.475], "Focus": [17741.5], "XYStage": [ 4775.5, 293.90000000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos33", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.445], "Focus": [17741.4], "XYStage": [ 3954.9, 293.90000000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos34", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.455], "Focus": [17741.4], "XYStage": [ -1324.9, 545.6 ] }, "GridColumnIndex": 0 }, { "Label": "Pos35", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.445], "Focus": [17741.3], "XYStage": [ -2097, 576.6 ] }, "GridColumnIndex": 0 } ], "z-step_um": 10, "GridRow": 0, "BitDepth": 16, "Depth": 2, "Channels": 6, "Source": "Micro-Manager", "ChColors": [ -16711681, -256, -65281, -1, -1, -16776961 ], "MetadataVersion": 10, "KeepShutterOpenChannels": true, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536, 65536 ], "CustomIntervals_ms": [], "Height": 1200, "MicroManagerVersion": "1.4.23 20180219", "GridColumn": 0, "UUID": "14a13bf7-c944-4672-b873-fcfbbb7ea13d", "PixelType": "GRAY16", "Width": 1200, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\Yan\\20191218-YC113-S7S8-miseq", "Frames": 1, "Comment": "", "UserName": "Nikon", "PixelSize_um": 1.1, "ROI": [ 0, 0, 1200, 1200 ], "Date": "2019-12-18", "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC", "P-DAPI-high" ], "KeepShutterOpenSlices": true, "IJType": 1, "ComputerName": "ZadorConfocal2", "Positions": 36, "PositionIndex": 9, "CameraTimeout": 20000, "TimeFirst": false }, "640 AO-Name": "AnalogIO", "Vincent-D3-Command": "Close" }, "FrameKey-0-5-10": { "COM4-StopBits": "1", "528 AO-MaxVolts": "5.0000", "XLIGHT Excitation Wheel-State": "1", "470 Shutter-Name": "AnalogIO", "Core-Shutter": "405", "555 AO-Demo": "No", "XLIGHT Dichroic-State": "2", "XYStage-StepSize-Y": "0.1000", "ZPositionUm": 298.82, "XYStage-StepSize-X": "0.1000", "PRIME-FirmwareVersion": "20.28", "PRIME-ClearCycles": "2", "520-Name": "DA Shutter", "405 Shutter-Name": "AnalogIO", "PIEZO-StepSize": "0.0050", "PRIME-ClearMode": "Pre-Exposure", "470 AO-MinVolts": "0.0000", "405 AO-SupportsTriggering": "1", "CameraChannelIndex": 0, "XYStage-StartSpeed": "500.0000", "COM9-StopBits": "2", "405 AO-Description": "NI DAC", "COM4-DelayBetweenCharsMs": "0.0000", "445 AO-Block voltage": "false", "520 AO-TriggerInputLine": "/Dev1/PFI0", "555 AO-Description": "NI DAC", "520 AO-TriggerSampleRate": "100000", "COM4-Verbose": "1", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM4-Name": "COM4", "405 Shutter-Volts": "5.0000", "Vincent-D3-Port": "COM1", "PRIME-PP 4 MIN ADU AFFECTED": "200", "470 Shutter-MinVolts": "0.0000", "PRIME-PP 3 ENABLED": "Yes", "470 AO-Description": "NI DAC", "PRIME-Description": "PVCAM API device adapter", "520 Shutter-MaxVolts": "5.0000", "520 Shutter-Volts": "0.0000", "528 AO-Sequenceable": "1", "PRIME-Binning": "1x1", "555 Shutter-MaxVolts": "5.0000", "528 Shutter-TriggerSequenceLength": "1000", "405 Shutter-Block voltage": "false", "XLIGHT Spin motor-State": "1", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "Vincent-D3-Time to open (ms)": "35.0000", "Vincent-D3-Time to close (ms)": "35.0000", "445 AO-TriggerSampleRate": "100000", "640 Shutter-IOChannel": "Dev1/ao14", "555 AO-Sequenceable": "1", "520 AO-Name": "AnalogIO", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "470 AO-Block voltage": "false", "COM1-Verbose": "1", "COM9-AnswerTimeout": "10000.0000", "528 Shutter-Name": "AnalogIO", "PRIME-Trigger Timeout (secs)": "10", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "NextFrame": 0, "470 AO-TriggerSequenceLength": "1000", "PRIME-TransposeXY": "0", "445 AO-TriggerSequenceLength": "1000", "COM1-Name": "COM1", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "COM9-DataBits": "8", "COM1-AnswerTimeout": "500.0000", "Camera": "", "TE2000-Version": "V2.50-V2.50-V2.10", "528 Shutter-Volts": "0.0000", "XLIGHT Hub-Name": "XLIGHT Hub", "PIEZO-Description": "Ludl stage driver adapter", "PositionName": "Pos9", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSampleRate": "100000", "PRIME-FanSpeedSetpoint": "High", "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "405 AO-TriggerSampleRate": "100000", "470 Shutter-Block voltage": "false", "470-State": "0", "COM3-Parity": "None", "405 AO-Volts": "4.5000", "PRIME-TransposeMirrorY": "0", "PRIME-ChipName": "GS144BSI", "470 AO-Volts": "0.0000", "PRIME-TransposeMirrorX": "0", "445 Shutter-SupportsTriggering": "0", "470 Shutter-SupportsTriggering": "0", "XLIGHT Hub-Description": "XLIGHT hub", "PRIME-PreampOffLimit": "10000", "470 AO-Sequenceable": "1", "528 Shutter-MaxVolts": "5.0000", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "Core-XYStage": "XYStage", "470 AO-TriggerSampleRate": "100000", "PRIME-Timing-PreTriggerDelayNs": "24672000.0000", "528-DA Device": "528 Shutter", "Focus-Description": "Nikon TE2000 focus adapter", "FilterBlock-State": "5", "520 AO-Demo": "No", "COM3-DelayBetweenCharsMs": "0.0000", "470 Shutter-Volts": "0.0000", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "PRIME-PP 2 THRESHOLD": "125", "PRIME-PP 4 THRESHOLD": "75", "XYStage-Acceleration": "4.0000", "470 Shutter-Demo": "No", "PRIME-Name": "PMPCIECam00", "640-DA Device": "640 Shutter", "405 AO-Block voltage": "false", "ElapsedTime-ms": 412639, "PRIME-Offset": "100", "445 AO-IOChannel": "Dev1/ao1", "640 AO-MaxVolts": "5.0000", "PositionIndex": 9, "PRIME-PP 1 ENABLED": "Yes", "COM1-BaudRate": "9600", "640-State": "0", "COM3-Verbose": "1", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "LudlController-TransmissionDelay": "0", "PRIME-FullWellCapacity": "62000", "PRIME-SMARTStreamingEnabled": "No", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "640 Shutter-Description": "NI DAC", "528-Description": "DA device that is used as a shutter", "COM4-Description": "Serial port driver (boost:asio)", "405 Shutter-Description": "NI DAC", "TE2000-Name": "TE2000", "528 Shutter-Description": "NI DAC", "COM9-Description": "Serial port driver (boost:asio)", "LudlController-Name": "LudlController", "445 Shutter-TriggerSequenceLength": "1000", "PRIME-SerialNumber": "A18B203008", "445 AO-Volts": "0.0000", "PRIME-X-dimension": "1200", "PRIME-ShutterCloseDelay": "0", "XLIGHT Disk slider-State": "2", "Position": "Default", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 AO-IOChannel": "Dev1/ao5", "520 AO-IOChannel": "Dev1/ao4", "528 AO-TriggerSequenceLength": "1000", "405 Shutter-MaxVolts": "5.0000", "470-DA Device": "470 Shutter", "PRIME-FTCapable": "No", "Core-ChannelGroup": "Channels", "555 AO-SupportsTriggering": "0", "PRIME-Y-dimension": "1200", "Frame": 0, "555 Shutter-Sequenceable": "1", "PRIME-Timing-ExposureTimeNs": "499998000.0000", "Core-ImageProcessor": "", "528-State": "0", "520 AO-Block voltage": "false", "XLIGHT Excitation Wheel-Label": "QUAD", "640 Shutter-Demo": "No", "COM3-StopBits": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "520 Shutter-IOChannel": "Dev1/ao11", "Width": 1200, "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-SupportsTriggering": "0", "640 AO-TriggerSampleRate": "100000", "520-Description": "DA device that is used as a shutter", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "405 Shutter-Sequenceable": "1", "405 Shutter-SupportsTriggering": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-TriggerSampleRate": "100000", "640 Shutter-MaxVolts": "5.0000", "555 AO-MaxVolts": "5.0000", "520 AO-TriggerSequenceLength": "1000", "COM3-BaudRate": "9600", "COM9-BaudRate": "9600", "Vincent-D3-Channel #": "Ch. #1", "PRIME-PMode": "Normal", "OpticalPath-Label": "Left(100)", "BitDepth": 16, "555 AO-TriggerSequenceLength": "1000", "445 Shutter-Description": "NI DAC", "405 AO-Sequenceable": "1", "PRIME-AcquisitionMethod": "Callbacks", "555 AO-Name": "AnalogIO", "PRIME-ShutterMode": "Pre-Exposure", "COM3-Handshaking": "Off", "445 AO-SupportsTriggering": "0", "ROI": "0-0-1200-1200", "COM9-Verbose": "1", "PRIME-Color": "OFF", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "PRIME-ShutterOpenDelay": "0", "COM1-Parity": "None", "Slice": 10, "SlicePosition": 50, "COM9-DelayBetweenCharsMs": "0.0000", "555-Name": "DA Shutter", "PIEZO-LudlSingleAxisName": "B", "XLIGHT Emission Wheel-Label": "QUAD", "640 Shutter-Name": "AnalogIO", "640 AO-Sequenceable": "1", "520 Shutter-Demo": "No", "528 Shutter-Demo": "No", "470-Name": "DA Shutter", "520-DA Device": "520 Shutter", "XYStage-Description": "Ludl XY stage driver adapter", "445 Shutter-MinVolts": "0.0000", "528 AO-TriggerSampleRate": "100000", "405 AO-MinVolts": "0.0000", "405 Shutter-TriggerSequenceLength": "1000", "Height": 1200, "555 AO-Block voltage": "false", "640 Shutter-Volts": "0.0000", "PixelType": "GRAY16", "445 Shutter-Block voltage": "false", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "520 Shutter-TriggerSequenceLength": "1000", "COM9-Name": "COM9", "Core-SLM": "", "555 AO-Volts": "0.0000", "555 Shutter-Block voltage": "false", "XYStage-Speed": "1800.0000", "520 AO-MaxVolts": "5.0000", "528 Shutter-SupportsTriggering": "0", "COM1-Description": "Serial port driver (boost:asio)", "PRIME-MetadataEnabled": "No", "520 Shutter-MinVolts": "0.0000", "470 Shutter-Sequenceable": "1", "PRIME-PreampDelay": "0", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-MinVolts": "0.0000", "ChannelIndex": 5, "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Core-AutoShutter": "1", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "Exposure-ms": 300, "PRIME-PP 2 ENABLED": "Yes", "640 Shutter-TriggerSampleRate": "100000", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "470 Shutter-IOChannel": "Dev1/ao10", "405 AO-Demo": "No", "PixelSizeUm": 1.1, "470 AO-Demo": "No", "470 Shutter-MaxVolts": "5.0000", "Vincent-D3-Name": "Vincent-D3", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FileName": "img_000000000_P-DAPI-high_010.tif", "LudlController-Reset": "Operate", "PRIME-Exposure": "300.00", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "445-Name": "DA Shutter", "555 Shutter-Name": "AnalogIO", "520 Shutter-TriggerSampleRate": "100000", "445 Shutter-IOChannel": "Dev1/ao9", "PRIME-TransposeCorrection": "0", "XYStage-StepSize": "0.1000", "445-Description": "DA device that is used as a shutter", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 AO-IOChannel": "Dev1/ao7", "520 Shutter-Name": "AnalogIO", "COM3-DataBits": "8", "640 AO-Volts": "0.0000", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "555 Shutter-IOChannel": "Dev1/ao13", "WaitInterval": null, "520 Shutter-Sequenceable": "1", "YPositionUm": 31506.2, "445-State": "0", "445-DA Device": "445 Shutter", "PRIME-CircularBufferAutoSize": "ON", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "555 AO-TriggerSampleRate": "100000", "445 Shutter-Volts": "0.0000", "XLIGHT Hub-Port": "COM3", "Core-AutoFocus": "", "COM4-Handshaking": "Off", "528 Shutter-MinVolts": "0.0000", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "445 Shutter-Name": "AnalogIO", "528 Shutter-Sequenceable": "1", "XYStage-TransposeMirrorX": "0", "XYStage-TransposeMirrorY": "0", "PRIME-ReadoutRate": "100MHz 16bit", "XYStage-Name": "Stage", "640 AO-SupportsTriggering": "0", "640-Name": "DA Shutter", "640 Shutter-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "640 AO-Block voltage": "false", "445 Shutter-Sequenceable": "1", "470 AO-MaxVolts": "5.0000", "COM4-BaudRate": "9600", "470-Description": "DA device that is used as a shutter", "520 Shutter-Description": "NI DAC", "PRIME-CCDTemperature": "-14.8500", "Time": "2019-12-18 21:37:20 -0500", "640 AO-TriggerSequenceLength": "1000", "470 AO-Name": "AnalogIO", "XLIGHT Disk slider-Label": "Disk Pos 40um", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "Binning": "1x1", "TE2000-ModelType": "1", "COM3-AnswerTimeout": "500.0000", "555 Shutter-SupportsTriggering": "0", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "TE2000-Port": "COM4", "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Description": "NI DAC", "COM4-AnswerTimeout": "500.0000", "COM1-DelayBetweenCharsMs": "0.0000", "FilterBlock-Label": "Confocal", "445 AO-Name": "AnalogIO", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-Timing-ReadoutTimeNs": "24672000.0000", "528 AO-Demo": "No", "520 AO-Sequenceable": "1", "555-Description": "DA device that is used as a shutter", "405-DA Device": "405 Shutter", "405 AO-TriggerSequenceLength": "1000", "640 AO-MinVolts": "0.0000", "640 Shutter-Block voltage": "false", "FrameIndex": 0, "PRIME-Port": "CMOS", "555 AO-MinVolts": "0.0000", "COM3-Description": "Serial port driver (boost:asio)", "528 AO-Description": "NI DAC", "555 AO-IOChannel": "Dev1/ao6", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "555 AO-TriggerInputLine": "/Dev1/PFI0", "PRIME-ActualInterval-ms": "390.5200", "LudlController-Port": "COM9", "445 Shutter-Demo": "No", "PRIME-Trigger-Expose Out-Mux": "1", "SliceIndex": 10, "PRIME-CCDTemperatureSetPoint": "-15.0000", "405 Shutter-MinVolts": "0.0000", "PRIME-ExposeOutMode": "First Row", "AxisPositions": null, "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Volts": "0.0000", "528-Name": "DA Shutter", "555-State": "0", "XLIGHT Dichroic-Label": "QUAD", "445 AO-Sequenceable": "1", "405-Name": "DA Shutter", "405 AO-IOChannel": "Dev1/ao0", "Core-Camera": "PRIME", "Focus-Name": "Focus", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "405 Shutter-IOChannel": "Dev1/ao8", "640 AO-Demo": "No", "640 Shutter-SupportsTriggering": "0", "445 AO-Demo": "No", "555-DA Device": "555 Shutter", "470 Shutter-Description": "NI DAC", "528 AO-Volts": "0.0000", "COM1-DataBits": "8", "Vincent-D3-Address": "x", "520 AO-SupportsTriggering": "0", "405-Description": "DA device that is used as a shutter", "640 Shutter-MinVolts": "0.0000", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "405 AO-Name": "AnalogIO", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "640 AO-TriggerInputLine": "/Dev1/PFI0", "405 AO-TriggerInputLine": "/Dev1/PFI0", "640-Description": "DA device that is used as a shutter", "445 Shutter-TriggerSampleRate": "100000", "470 Shutter-TriggerSequenceLength": "1000", "COM1-StopBits": "1", "520 Shutter-SupportsTriggering": "0", "405-State": "0", "Core-Initialize": "1", "XLIGHT Emission Wheel-State": "3", "520 AO-Description": "NI DAC", "528 AO-TriggerInputLine": "/Dev1/PFI0", "445 Shutter-MaxVolts": "5.0000", "COM9-Handshaking": "Off", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "XLIGHT Spin motor-Label": "On (spinning)", "PRIME-PP 5 ENABLED": "No", "UUID": "c4d3acc2-eb63-4bd3-ada3-5c4ac7728ac8", "PRIME-TriggerMode": "Internal Trigger", "COM1-Handshaking": "Off", "PRIME-Timing-PostTriggerDelayNs": "49344000.0000", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "Focus-Position": "17741.5000", "528 Shutter-Block voltage": "false", "Channel": "P-DAPI-high", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "555 Shutter-Demo": "No", "405 Shutter-Demo": "No", "528 AO-Name": "AnalogIO", "520-State": "0", "Core-Galvo": "", "405 Shutter-TriggerSampleRate": "100000", "Focus-FocusStepSize_(nm)": "100", "PRIME-PixelType": "16bit", "COM4-DataBits": "8", "470 AO-IOChannel": "Dev1/ao2", "520 AO-MinVolts": "0.0000", "COM9-Parity": "None", "PRIME-CircularBufferEnabled": "ON", "PRIME-PP 6 Reset": "No", "640 AO-Description": "NI DAC", "Source": "PRIME", "445 AO-MinVolts": "0.0000", "PIEZO-Name": "Stage", "445 AO-Description": "NI DAC", "PRIME-CircularBufferFrameCount": "46", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "520 AO-Volts": "0.0000", "528 Shutter-TriggerSampleRate": "100000", "XPositionUm": 19731.9, "OpticalPath-State": "4", "PRIME-PP 3 THRESHOLD": "80", "PRIME-PVCAM Version": "3.7.1", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Gain": "1-HDR", "640 Shutter-TriggerSequenceLength": "1000", "528 AO-SupportsTriggering": "0", "445 AO-TriggerInputLine": "/Dev1/PFI0", "OpticalPath-Name": "OpticalPath", "PRIME-PP 4 ENABLED": "Yes", "PRIME-PVCAM Adapter Version": "1.3.29", "COM3-Name": "COM3", "405 AO-MaxVolts": "5.0000", "520 Shutter-Block voltage": "false", "PRIME-Timing-ClearingTimeNs": "49344000.0000", "Core-Focus": "PIEZO", "528 AO-Block voltage": "false", "TE2000-Description": "Nikon TE2000 hub", "528 AO-MinVolts": "0.0000", "COM4-Parity": "None", "Summary": { "Slices": 11, "ChContrastMin": [ 0, 0, 0, 0, 0, 0 ], "PixelAspect": 1, "SlicesFirst": true, "Interval_ms": 0, "Time": "2019-12-18 21:36:42 -0500", "Prefix": "seq_4", "InitialPositionList": [ { "Label": "Pos0", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.21], "Focus": [17741.5], "XYStage": [ 44542.200000000004, 31636.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos1", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.22], "Focus": [17741.4], "XYStage": [ 43801.100000000006, 31637.9 ] }, "GridColumnIndex": 0 }, { "Label": "Pos2", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.5], "XYStage": [ 38561.3, 32040.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos3", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.185], "Focus": [17741.3], "XYStage": [ 37698.4, 32041.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos4", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.16], "Focus": [17741.5], "XYStage": [ 32375.4, 31758.9 ] }, "GridColumnIndex": 0 }, { "Label": "Pos5", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.17000000000002], "Focus": [17741.3], "XYStage": [ 31770.7, 31758.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos6", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.4], "XYStage": [ 26536.800000000003, 31068.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos7", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.205], "Focus": [17741.4], "XYStage": [ 25420.2, 31068.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos8", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.85], "Focus": [17741.3], "XYStage": [ 21059, 31508 ] }, "GridColumnIndex": 0 }, { "Label": "Pos9", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.82], "Focus": [17741.3], "XYStage": [ 19731.9, 31506.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos10", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.79], "Focus": [17741.4], "XYStage": [ 14528.5, 32007.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos11", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.83], "Focus": [17741.2], "XYStage": [ 13830.300000000001, 32009.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos12", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.79], "Focus": [17741.3], "XYStage": [ 8775.300000000001, 32017.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos13", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.815], "Focus": [17741.3], "XYStage": [ 8109.200000000001, 32008.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos14", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.805], "Focus": [17741.3], "XYStage": [ 3483.3, 31908.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos15", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.82], "Focus": [17741.2], "XYStage": [ 2686.8, 31903.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos16", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [211.48000000000002], "Focus": [17741.1], "XYStage": [ -1978.9, 31973.600000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos17", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [211.51500000000001], "Focus": [17741.1], "XYStage": [ -2957.8, 31982.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos18", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.195], "Focus": [17741.5], "XYStage": [ 47747.4, -576.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos19", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.23000000000002], "Focus": [17741.6], "XYStage": [ 47053.9, -576.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos20", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.205], "Focus": [17741.4], "XYStage": [ 41871.200000000004, -479.8 ] }, "GridColumnIndex": 0 }, { "Label": "Pos21", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.4], "XYStage": [ 40882.600000000006, -392 ] }, "GridColumnIndex": 0 }, { "Label": "Pos22", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.24], "Focus": [17741.4], "XYStage": [ 35905.6, -135.3 ] }, "GridColumnIndex": 0 }, { "Label": "Pos23", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.195], "Focus": [17741.5], "XYStage": [ 34990.9, -132.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos24", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.46], "Focus": [17741.4], "XYStage": [ 29678.100000000002, -958.1 ] }, "GridColumnIndex": 0 }, { "Label": "Pos25", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.44], "Focus": [17741.5], "XYStage": [ 28983.600000000002, -939.4000000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos26", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.475], "Focus": [17741.5], "XYStage": [ 23019.7, -122 ] }, "GridColumnIndex": 0 }, { "Label": "Pos27", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.44], "Focus": [17741.7], "XYStage": [ 22428, -116.80000000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos28", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.46], "Focus": [17741.7], "XYStage": [ 16364.900000000001, -181.10000000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos29", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.48000000000002], "Focus": [17741.5], "XYStage": [ 15712.1, -180 ] }, "GridColumnIndex": 0 }, { "Label": "Pos30", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.45000000000002], "Focus": [17741.7], "XYStage": [ 10346.1, 77.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos31", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.49], "Focus": [17741.4], "XYStage": [ 9755.5, 85.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos32", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.475], "Focus": [17741.5], "XYStage": [ 4775.5, 293.90000000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos33", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.445], "Focus": [17741.4], "XYStage": [ 3954.9, 293.90000000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos34", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.455], "Focus": [17741.4], "XYStage": [ -1324.9, 545.6 ] }, "GridColumnIndex": 0 }, { "Label": "Pos35", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.445], "Focus": [17741.3], "XYStage": [ -2097, 576.6 ] }, "GridColumnIndex": 0 } ], "z-step_um": 10, "GridRow": 0, "BitDepth": 16, "Depth": 2, "Channels": 6, "Source": "Micro-Manager", "ChColors": [ -16711681, -256, -65281, -1, -1, -16776961 ], "MetadataVersion": 10, "KeepShutterOpenChannels": true, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536, 65536 ], "CustomIntervals_ms": [], "Height": 1200, "MicroManagerVersion": "1.4.23 20180219", "GridColumn": 0, "UUID": "14a13bf7-c944-4672-b873-fcfbbb7ea13d", "PixelType": "GRAY16", "Width": 1200, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\Yan\\20191218-YC113-S7S8-miseq", "Frames": 1, "Comment": "", "UserName": "Nikon", "PixelSize_um": 1.1, "ROI": [ 0, 0, 1200, 1200 ], "Date": "2019-12-18", "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC", "P-DAPI-high" ], "KeepShutterOpenSlices": true, "IJType": 1, "ComputerName": "ZadorConfocal2", "Positions": 36, "PositionIndex": 9, "CameraTimeout": 20000, "TimeFirst": false }, "640 AO-Name": "AnalogIO", "Vincent-D3-Command": "Close" } }