{ "Summary": { "Slices": 11, "ChContrastMin": [ 0, 0, 0, 0, 0, 0 ], "PixelAspect": 1, "SlicesFirst": true, "Interval_ms": 0, "Time": "2019-12-18 23:10:50 -0500", "Prefix": "seq_5", "InitialPositionList": [ { "Label": "Pos0", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.21], "Focus": [17741.5], "XYStage": [ 44542.200000000004, 31636.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos1", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.22], "Focus": [17741.4], "XYStage": [ 43801.100000000006, 31637.9 ] }, "GridColumnIndex": 0 }, { "Label": "Pos2", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.5], "XYStage": [ 38561.3, 32040.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos3", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.185], "Focus": [17741.3], "XYStage": [ 37698.4, 32041.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos4", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.16], "Focus": [17741.5], "XYStage": [ 32375.4, 31758.9 ] }, "GridColumnIndex": 0 }, { "Label": "Pos5", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.17000000000002], "Focus": [17741.3], "XYStage": [ 31770.7, 31758.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos6", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.4], "XYStage": [ 26536.800000000003, 31068.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos7", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.205], "Focus": [17741.4], "XYStage": [ 25420.2, 31068.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos8", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.85], "Focus": [17741.3], "XYStage": [ 21059, 31508 ] }, "GridColumnIndex": 0 }, { "Label": "Pos9", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.82], "Focus": [17741.3], "XYStage": [ 19731.9, 31506.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos10", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.79], "Focus": [17741.4], "XYStage": [ 14528.5, 32007.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos11", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.83], "Focus": [17741.2], "XYStage": [ 13830.300000000001, 32009.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos12", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.79], "Focus": [17741.3], "XYStage": [ 8775.300000000001, 32017.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos13", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.815], "Focus": [17741.3], "XYStage": [ 8109.200000000001, 32008.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos14", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.805], "Focus": [17741.3], "XYStage": [ 3483.3, 31908.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos15", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.82], "Focus": [17741.2], "XYStage": [ 2686.8, 31903.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos16", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [211.48000000000002], "Focus": [17741.1], "XYStage": [ -1978.9, 31973.600000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos17", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [211.51500000000001], "Focus": [17741.1], "XYStage": [ -2957.8, 31982.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos18", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.195], "Focus": [17741.5], "XYStage": [ 47747.4, -576.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos19", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.23000000000002], "Focus": [17741.6], "XYStage": [ 47053.9, -576.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos20", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.205], "Focus": [17741.4], "XYStage": [ 41871.200000000004, -479.8 ] }, "GridColumnIndex": 0 }, { "Label": "Pos21", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.4], "XYStage": [ 40882.600000000006, -392 ] }, "GridColumnIndex": 0 }, { "Label": "Pos22", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.24], "Focus": [17741.4], "XYStage": [ 35905.6, -135.3 ] }, "GridColumnIndex": 0 }, { "Label": "Pos23", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.195], "Focus": [17741.5], "XYStage": [ 34990.9, -132.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos24", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.46], "Focus": [17741.4], "XYStage": [ 29678.100000000002, -958.1 ] }, "GridColumnIndex": 0 }, { "Label": "Pos25", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.44], "Focus": [17741.5], "XYStage": [ 28983.600000000002, -939.4000000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos26", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.475], "Focus": [17741.5], "XYStage": [ 23019.7, -122 ] }, "GridColumnIndex": 0 }, { "Label": "Pos27", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.44], "Focus": [17741.7], "XYStage": [ 22428, -116.80000000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos28", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.46], "Focus": [17741.7], "XYStage": [ 16364.900000000001, -181.10000000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos29", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.48000000000002], "Focus": [17741.5], "XYStage": [ 15712.1, -180 ] }, "GridColumnIndex": 0 }, { "Label": "Pos30", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.45000000000002], "Focus": [17741.7], "XYStage": [ 10346.1, 77.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos31", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.49], "Focus": [17741.4], "XYStage": [ 9755.5, 85.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos32", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.475], "Focus": [17741.5], "XYStage": [ 4775.5, 293.90000000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos33", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.445], "Focus": [17741.4], "XYStage": [ 3954.9, 293.90000000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos34", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.455], "Focus": [17741.4], "XYStage": [ -1324.9, 545.6 ] }, "GridColumnIndex": 0 }, { "Label": "Pos35", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.445], "Focus": [17741.3], "XYStage": [ -2097, 576.6 ] }, "GridColumnIndex": 0 } ], "z-step_um": 10, "GridRow": 0, "BitDepth": 16, "Depth": 2, "Channels": 6, "Source": "Micro-Manager", "ChColors": [ -16711681, -256, -65281, -1, -1, -16776961 ], "MetadataVersion": 10, "KeepShutterOpenChannels": true, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536, 65536 ], "CustomIntervals_ms": [], "Height": 1200, "MicroManagerVersion": "1.4.23 20180219", "GridColumn": 0, "UUID": "67598d21-5db5-4842-ab60-f506f7b42c7c", "PixelType": "GRAY16", "Width": 1200, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\Yan\\20191218-YC113-S7S8-miseq", "Frames": 1, "Comment": "", "UserName": "Nikon", "PixelSize_um": 1.1, "ROI": [ 0, 0, 1200, 1200 ], "Date": "2019-12-18", "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC", "P-DAPI-high" ], "KeepShutterOpenSlices": true, "IJType": 1, "ComputerName": "ZadorConfocal2", "Positions": 36, "PositionIndex": 29, "CameraTimeout": 20000, "TimeFirst": false }, "FrameKey-0-0-0": { "COM4-StopBits": "1", "528 AO-MaxVolts": "5.0000", "XLIGHT Excitation Wheel-State": "1", "470 Shutter-Name": "AnalogIO", "Core-Shutter": "520", "555 AO-Demo": "No", "XLIGHT Dichroic-State": "2", "XYStage-StepSize-Y": "0.1000", "ZPositionUm": 168.48000000000002, "XYStage-StepSize-X": "0.1000", "PRIME-FirmwareVersion": "20.28", "PRIME-ClearCycles": "2", "520-Name": "DA Shutter", "405 Shutter-Name": "AnalogIO", "PIEZO-StepSize": "0.0050", "PRIME-ClearMode": "Pre-Exposure", "470 AO-MinVolts": "0.0000", "405 AO-SupportsTriggering": "1", "CameraChannelIndex": 0, "XYStage-StartSpeed": "500.0000", "COM9-StopBits": "2", "405 AO-Description": "NI DAC", "COM4-DelayBetweenCharsMs": "0.0000", "445 AO-Block voltage": "false", "520 AO-TriggerInputLine": "/Dev1/PFI0", "555 AO-Description": "NI DAC", "520 AO-TriggerSampleRate": "100000", "COM4-Verbose": "1", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM4-Name": "COM4", "405 Shutter-Volts": "0.0000", "Vincent-D3-Port": "COM1", "PRIME-PP 4 MIN ADU AFFECTED": "200", "470 Shutter-MinVolts": "0.0000", "PRIME-PP 3 ENABLED": "Yes", "470 AO-Description": "NI DAC", "PRIME-Description": "PVCAM API device adapter", "520 Shutter-MaxVolts": "5.0000", "520 Shutter-Volts": "5.0000", "528 AO-Sequenceable": "1", "PRIME-Binning": "1x1", "555 Shutter-MaxVolts": "5.0000", "528 Shutter-TriggerSequenceLength": "1000", "405 Shutter-Block voltage": "false", "XLIGHT Spin motor-State": "1", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "Vincent-D3-Time to open (ms)": "35.0000", "Vincent-D3-Time to close (ms)": "35.0000", "445 AO-TriggerSampleRate": "100000", "640 Shutter-IOChannel": "Dev1/ao14", "555 AO-Sequenceable": "1", "520 AO-Name": "AnalogIO", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "470 AO-Block voltage": "false", "COM1-Verbose": "1", "COM9-AnswerTimeout": "10000.0000", "528 Shutter-Name": "AnalogIO", "PRIME-Trigger Timeout (secs)": "10", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "NextFrame": 0, "470 AO-TriggerSequenceLength": "1000", "PRIME-TransposeXY": "0", "445 AO-TriggerSequenceLength": "1000", "COM1-Name": "COM1", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "COM9-DataBits": "8", "COM1-AnswerTimeout": "500.0000", "Camera": "", "TE2000-Version": "V2.50-V2.50-V2.10", "528 Shutter-Volts": "0.0000", "XLIGHT Hub-Name": "XLIGHT Hub", "PIEZO-Description": "Ludl stage driver adapter", "PositionName": "Pos29", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSampleRate": "100000", "PRIME-FanSpeedSetpoint": "High", "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "405 AO-TriggerSampleRate": "100000", "470 Shutter-Block voltage": "false", "470-State": "0", "COM3-Parity": "None", "405 AO-Volts": "0.0000", "PRIME-TransposeMirrorY": "0", "PRIME-ChipName": "GS144BSI", "470 AO-Volts": "0.0000", "PRIME-TransposeMirrorX": "0", "445 Shutter-SupportsTriggering": "0", "470 Shutter-SupportsTriggering": "0", "XLIGHT Hub-Description": "XLIGHT hub", "PRIME-PreampOffLimit": "10000", "470 AO-Sequenceable": "1", "528 Shutter-MaxVolts": "5.0000", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "Core-XYStage": "XYStage", "470 AO-TriggerSampleRate": "100000", "PRIME-Timing-PreTriggerDelayNs": "24672000.0000", "528-DA Device": "528 Shutter", "Focus-Description": "Nikon TE2000 focus adapter", "FilterBlock-State": "5", "520 AO-Demo": "No", "COM3-DelayBetweenCharsMs": "0.0000", "470 Shutter-Volts": "0.0000", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "PRIME-PP 2 THRESHOLD": "125", "PRIME-PP 4 THRESHOLD": "75", "XYStage-Acceleration": "4.0000", "470 Shutter-Demo": "No", "PRIME-Name": "PMPCIECam00", "640-DA Device": "640 Shutter", "405 AO-Block voltage": "false", "ElapsedTime-ms": 1190220, "PRIME-Offset": "100", "445 AO-IOChannel": "Dev1/ao1", "640 AO-MaxVolts": "5.0000", "PositionIndex": 29, "PRIME-PP 1 ENABLED": "Yes", "COM1-BaudRate": "9600", "640-State": "0", "COM3-Verbose": "1", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "LudlController-TransmissionDelay": "0", "PRIME-FullWellCapacity": "62000", "PRIME-SMARTStreamingEnabled": "No", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "640 Shutter-Description": "NI DAC", "528-Description": "DA device that is used as a shutter", "COM4-Description": "Serial port driver (boost:asio)", "405 Shutter-Description": "NI DAC", "TE2000-Name": "TE2000", "528 Shutter-Description": "NI DAC", "COM9-Description": "Serial port driver (boost:asio)", "LudlController-Name": "LudlController", "445 Shutter-TriggerSequenceLength": "1000", "PRIME-SerialNumber": "A18B203008", "445 AO-Volts": "0.0000", "PRIME-X-dimension": "1200", "PRIME-ShutterCloseDelay": "0", "XLIGHT Disk slider-State": "2", "Position": "Default", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 AO-IOChannel": "Dev1/ao5", "520 AO-IOChannel": "Dev1/ao4", "528 AO-TriggerSequenceLength": "1000", "405 Shutter-MaxVolts": "5.0000", "470-DA Device": "470 Shutter", "PRIME-FTCapable": "No", "Core-ChannelGroup": "Channels", "555 AO-SupportsTriggering": "0", "PRIME-Y-dimension": "1200", "Frame": 0, "555 Shutter-Sequenceable": "1", "PRIME-Timing-ExposureTimeNs": "499998000.0000", "Core-ImageProcessor": "", "528-State": "0", "520 AO-Block voltage": "false", "XLIGHT Excitation Wheel-Label": "CFP/YFP", "640 Shutter-Demo": "No", "COM3-StopBits": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "520 Shutter-IOChannel": "Dev1/ao11", "Width": 1200, "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-SupportsTriggering": "0", "640 AO-TriggerSampleRate": "100000", "520-Description": "DA device that is used as a shutter", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "405 Shutter-Sequenceable": "1", "405 Shutter-SupportsTriggering": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-TriggerSampleRate": "100000", "640 Shutter-MaxVolts": "5.0000", "555 AO-MaxVolts": "5.0000", "520 AO-TriggerSequenceLength": "1000", "COM3-BaudRate": "9600", "COM9-BaudRate": "9600", "Vincent-D3-Channel #": "Ch. #1", "PRIME-PMode": "Normal", "OpticalPath-Label": "Left(100)", "BitDepth": 16, "555 AO-TriggerSequenceLength": "1000", "445 Shutter-Description": "NI DAC", "405 AO-Sequenceable": "1", "PRIME-AcquisitionMethod": "Callbacks", "555 AO-Name": "AnalogIO", "PRIME-ShutterMode": "Pre-Exposure", "COM3-Handshaking": "Off", "445 AO-SupportsTriggering": "0", "ROI": "0-0-1200-1200", "COM9-Verbose": "1", "PRIME-Color": "OFF", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "PRIME-ShutterOpenDelay": "0", "COM1-Parity": "None", "Slice": 0, "SlicePosition": -50, "COM9-DelayBetweenCharsMs": "0.0000", "555-Name": "DA Shutter", "PIEZO-LudlSingleAxisName": "B", "XLIGHT Emission Wheel-Label": "565/24(G)", "640 Shutter-Name": "AnalogIO", "640 AO-Sequenceable": "1", "520 Shutter-Demo": "No", "528 Shutter-Demo": "No", "470-Name": "DA Shutter", "520-DA Device": "520 Shutter", "XYStage-Description": "Ludl XY stage driver adapter", "445 Shutter-MinVolts": "0.0000", "528 AO-TriggerSampleRate": "100000", "405 AO-MinVolts": "0.0000", "405 Shutter-TriggerSequenceLength": "1000", "Height": 1200, "555 AO-Block voltage": "false", "640 Shutter-Volts": "0.0000", "PixelType": "GRAY16", "445 Shutter-Block voltage": "false", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "520 Shutter-TriggerSequenceLength": "1000", "COM9-Name": "COM9", "Core-SLM": "", "555 AO-Volts": "0.0000", "555 Shutter-Block voltage": "false", "XYStage-Speed": "1800.0000", "520 AO-MaxVolts": "5.0000", "528 Shutter-SupportsTriggering": "0", "COM1-Description": "Serial port driver (boost:asio)", "PRIME-MetadataEnabled": "No", "520 Shutter-MinVolts": "0.0000", "470 Shutter-Sequenceable": "1", "PRIME-PreampDelay": "0", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-MinVolts": "0.0000", "ChannelIndex": 0, "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Core-AutoShutter": "1", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "Exposure-ms": 320, "PRIME-PP 2 ENABLED": "Yes", "640 Shutter-TriggerSampleRate": "100000", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "470 Shutter-IOChannel": "Dev1/ao10", "405 AO-Demo": "No", "PixelSizeUm": 1.1, "470 AO-Demo": "No", "470 Shutter-MaxVolts": "5.0000", "Vincent-D3-Name": "Vincent-D3", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FileName": "img_000000000_Il-G_000.tif", "LudlController-Reset": "Operate", "PRIME-Exposure": "320.00", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "445-Name": "DA Shutter", "555 Shutter-Name": "AnalogIO", "520 Shutter-TriggerSampleRate": "100000", "445 Shutter-IOChannel": "Dev1/ao9", "PRIME-TransposeCorrection": "0", "XYStage-StepSize": "0.1000", "445-Description": "DA device that is used as a shutter", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 AO-IOChannel": "Dev1/ao7", "520 Shutter-Name": "AnalogIO", "COM3-DataBits": "8", "640 AO-Volts": "0.0000", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "555 Shutter-IOChannel": "Dev1/ao13", "WaitInterval": null, "520 Shutter-Sequenceable": "1", "YPositionUm": -180, "445-State": "0", "445-DA Device": "445 Shutter", "PRIME-CircularBufferAutoSize": "ON", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "555 AO-TriggerSampleRate": "100000", "445 Shutter-Volts": "0.0000", "XLIGHT Hub-Port": "COM3", "Core-AutoFocus": "", "COM4-Handshaking": "Off", "528 Shutter-MinVolts": "0.0000", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "445 Shutter-Name": "AnalogIO", "528 Shutter-Sequenceable": "1", "XYStage-TransposeMirrorX": "0", "XYStage-TransposeMirrorY": "0", "PRIME-ReadoutRate": "100MHz 16bit", "XYStage-Name": "Stage", "640 AO-SupportsTriggering": "0", "640-Name": "DA Shutter", "640 Shutter-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "640 AO-Block voltage": "false", "445 Shutter-Sequenceable": "1", "470 AO-MaxVolts": "5.0000", "COM4-BaudRate": "9600", "470-Description": "DA device that is used as a shutter", "520 Shutter-Description": "NI DAC", "PRIME-CCDTemperature": "-14.8500", "Time": "2019-12-18 23:10:50 -0500", "640 AO-TriggerSequenceLength": "1000", "470 AO-Name": "AnalogIO", "XLIGHT Disk slider-Label": "Disk Pos 40um", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "Binning": "1x1", "TE2000-ModelType": "1", "COM3-AnswerTimeout": "500.0000", "555 Shutter-SupportsTriggering": "0", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "TE2000-Port": "COM4", "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Description": "NI DAC", "COM4-AnswerTimeout": "500.0000", "COM1-DelayBetweenCharsMs": "0.0000", "FilterBlock-Label": "Confocal", "445 AO-Name": "AnalogIO", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-Timing-ReadoutTimeNs": "24672000.0000", "528 AO-Demo": "No", "520 AO-Sequenceable": "1", "555-Description": "DA device that is used as a shutter", "405-DA Device": "405 Shutter", "405 AO-TriggerSequenceLength": "1000", "640 AO-MinVolts": "0.0000", "640 Shutter-Block voltage": "false", "FrameIndex": 0, "PRIME-Port": "CMOS", "555 AO-MinVolts": "0.0000", "COM3-Description": "Serial port driver (boost:asio)", "528 AO-Description": "NI DAC", "555 AO-IOChannel": "Dev1/ao6", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "555 AO-TriggerInputLine": "/Dev1/PFI0", "PRIME-ActualInterval-ms": "390.5200", "LudlController-Port": "COM9", "445 Shutter-Demo": "No", "PRIME-Trigger-Expose Out-Mux": "1", "SliceIndex": 0, "PRIME-CCDTemperatureSetPoint": "-15.0000", "405 Shutter-MinVolts": "0.0000", "PRIME-ExposeOutMode": "First Row", "AxisPositions": null, "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Volts": "0.0000", "528-Name": "DA Shutter", "555-State": "0", "XLIGHT Dichroic-Label": "CFP/YFP", "445 AO-Sequenceable": "1", "405-Name": "DA Shutter", "405 AO-IOChannel": "Dev1/ao0", "Core-Camera": "PRIME", "Focus-Name": "Focus", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "405 Shutter-IOChannel": "Dev1/ao8", "640 AO-Demo": "No", "640 Shutter-SupportsTriggering": "0", "445 AO-Demo": "No", "555-DA Device": "555 Shutter", "470 Shutter-Description": "NI DAC", "528 AO-Volts": "0.0000", "COM1-DataBits": "8", "Vincent-D3-Address": "x", "520 AO-SupportsTriggering": "0", "405-Description": "DA device that is used as a shutter", "640 Shutter-MinVolts": "0.0000", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "405 AO-Name": "AnalogIO", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "640 AO-TriggerInputLine": "/Dev1/PFI0", "405 AO-TriggerInputLine": "/Dev1/PFI0", "640-Description": "DA device that is used as a shutter", "445 Shutter-TriggerSampleRate": "100000", "470 Shutter-TriggerSequenceLength": "1000", "COM1-StopBits": "1", "520 Shutter-SupportsTriggering": "0", "405-State": "0", "Core-Initialize": "1", "XLIGHT Emission Wheel-State": "3", "520 AO-Description": "NI DAC", "528 AO-TriggerInputLine": "/Dev1/PFI0", "445 Shutter-MaxVolts": "5.0000", "COM9-Handshaking": "Off", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "XLIGHT Spin motor-Label": "On (spinning)", "PRIME-PP 5 ENABLED": "No", "UUID": "cbe4cf54-533c-4dcc-95e5-6f193251992a", "PRIME-TriggerMode": "Internal Trigger", "COM1-Handshaking": "Off", "PRIME-Timing-PostTriggerDelayNs": "49344000.0000", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "Focus-Position": "17741.5000", "528 Shutter-Block voltage": "false", "Channel": "Il-G", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "555 Shutter-Demo": "No", "405 Shutter-Demo": "No", "528 AO-Name": "AnalogIO", "520-State": "1", "Core-Galvo": "", "405 Shutter-TriggerSampleRate": "100000", "Focus-FocusStepSize_(nm)": "100", "PRIME-PixelType": "16bit", "COM4-DataBits": "8", "470 AO-IOChannel": "Dev1/ao2", "520 AO-MinVolts": "0.0000", "COM9-Parity": "None", "PRIME-CircularBufferEnabled": "ON", "PRIME-PP 6 Reset": "No", "640 AO-Description": "NI DAC", "Source": "PRIME", "445 AO-MinVolts": "0.0000", "PIEZO-Name": "Stage", "445 AO-Description": "NI DAC", "PRIME-CircularBufferFrameCount": "46", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "520 AO-Volts": "4.5000", "528 Shutter-TriggerSampleRate": "100000", "XPositionUm": 15712.1, "OpticalPath-State": "4", "PRIME-PP 3 THRESHOLD": "80", "PRIME-PVCAM Version": "3.7.1", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Gain": "1-HDR", "640 Shutter-TriggerSequenceLength": "1000", "528 AO-SupportsTriggering": "0", "445 AO-TriggerInputLine": "/Dev1/PFI0", "OpticalPath-Name": "OpticalPath", "PRIME-PP 4 ENABLED": "Yes", "PRIME-PVCAM Adapter Version": "1.3.29", "COM3-Name": "COM3", "405 AO-MaxVolts": "5.0000", "520 Shutter-Block voltage": "false", "PRIME-Timing-ClearingTimeNs": "49344000.0000", "Core-Focus": "PIEZO", "528 AO-Block voltage": "false", "TE2000-Description": "Nikon TE2000 hub", "528 AO-MinVolts": "0.0000", "COM4-Parity": "None", "Summary": { "Slices": 11, "ChContrastMin": [ 0, 0, 0, 0, 0, 0 ], "PixelAspect": 1, "SlicesFirst": true, "Interval_ms": 0, "Time": "2019-12-18 23:10:50 -0500", "Prefix": "seq_5", "InitialPositionList": [ { "Label": "Pos0", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.21], "Focus": [17741.5], "XYStage": [ 44542.200000000004, 31636.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos1", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.22], "Focus": [17741.4], "XYStage": [ 43801.100000000006, 31637.9 ] }, "GridColumnIndex": 0 }, { "Label": "Pos2", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.5], "XYStage": [ 38561.3, 32040.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos3", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.185], "Focus": [17741.3], "XYStage": [ 37698.4, 32041.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos4", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.16], "Focus": [17741.5], "XYStage": [ 32375.4, 31758.9 ] }, "GridColumnIndex": 0 }, { "Label": "Pos5", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.17000000000002], "Focus": [17741.3], "XYStage": [ 31770.7, 31758.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos6", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.4], "XYStage": [ 26536.800000000003, 31068.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos7", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.205], "Focus": [17741.4], "XYStage": [ 25420.2, 31068.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos8", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.85], "Focus": [17741.3], "XYStage": [ 21059, 31508 ] }, "GridColumnIndex": 0 }, { "Label": "Pos9", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.82], "Focus": [17741.3], "XYStage": [ 19731.9, 31506.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos10", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.79], "Focus": [17741.4], "XYStage": [ 14528.5, 32007.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos11", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.83], "Focus": [17741.2], "XYStage": [ 13830.300000000001, 32009.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos12", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.79], "Focus": [17741.3], "XYStage": [ 8775.300000000001, 32017.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos13", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.815], "Focus": [17741.3], "XYStage": [ 8109.200000000001, 32008.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos14", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.805], "Focus": [17741.3], "XYStage": [ 3483.3, 31908.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos15", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.82], "Focus": [17741.2], "XYStage": [ 2686.8, 31903.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos16", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [211.48000000000002], "Focus": [17741.1], "XYStage": [ -1978.9, 31973.600000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos17", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [211.51500000000001], "Focus": [17741.1], "XYStage": [ -2957.8, 31982.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos18", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.195], "Focus": [17741.5], "XYStage": [ 47747.4, -576.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos19", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.23000000000002], "Focus": [17741.6], "XYStage": [ 47053.9, -576.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos20", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.205], "Focus": [17741.4], "XYStage": [ 41871.200000000004, -479.8 ] }, "GridColumnIndex": 0 }, { "Label": "Pos21", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.4], "XYStage": [ 40882.600000000006, -392 ] }, "GridColumnIndex": 0 }, { "Label": "Pos22", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.24], "Focus": [17741.4], "XYStage": [ 35905.6, -135.3 ] }, "GridColumnIndex": 0 }, { "Label": "Pos23", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.195], "Focus": [17741.5], "XYStage": [ 34990.9, -132.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos24", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.46], "Focus": [17741.4], "XYStage": [ 29678.100000000002, -958.1 ] }, "GridColumnIndex": 0 }, { "Label": "Pos25", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.44], "Focus": [17741.5], "XYStage": [ 28983.600000000002, -939.4000000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos26", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.475], "Focus": [17741.5], "XYStage": [ 23019.7, -122 ] }, "GridColumnIndex": 0 }, { "Label": "Pos27", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.44], "Focus": [17741.7], "XYStage": [ 22428, -116.80000000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos28", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.46], "Focus": [17741.7], "XYStage": [ 16364.900000000001, -181.10000000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos29", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.48000000000002], "Focus": [17741.5], "XYStage": [ 15712.1, -180 ] }, "GridColumnIndex": 0 }, { "Label": "Pos30", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.45000000000002], "Focus": [17741.7], "XYStage": [ 10346.1, 77.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos31", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.49], "Focus": [17741.4], "XYStage": [ 9755.5, 85.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos32", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.475], "Focus": [17741.5], "XYStage": [ 4775.5, 293.90000000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos33", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.445], "Focus": [17741.4], "XYStage": [ 3954.9, 293.90000000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos34", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.455], "Focus": [17741.4], "XYStage": [ -1324.9, 545.6 ] }, "GridColumnIndex": 0 }, { "Label": "Pos35", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.445], "Focus": [17741.3], "XYStage": [ -2097, 576.6 ] }, "GridColumnIndex": 0 } ], "z-step_um": 10, "GridRow": 0, "BitDepth": 16, "Depth": 2, "Channels": 6, "Source": "Micro-Manager", "ChColors": [ -16711681, -256, -65281, -1, -1, -16776961 ], "MetadataVersion": 10, "KeepShutterOpenChannels": true, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536, 65536 ], "CustomIntervals_ms": [], "Height": 1200, "MicroManagerVersion": "1.4.23 20180219", "GridColumn": 0, "UUID": "67598d21-5db5-4842-ab60-f506f7b42c7c", "PixelType": "GRAY16", "Width": 1200, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\Yan\\20191218-YC113-S7S8-miseq", "Frames": 1, "Comment": "", "UserName": "Nikon", "PixelSize_um": 1.1, "ROI": [ 0, 0, 1200, 1200 ], "Date": "2019-12-18", "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC", "P-DAPI-high" ], "KeepShutterOpenSlices": true, "IJType": 1, "ComputerName": "ZadorConfocal2", "Positions": 36, "PositionIndex": 29, "CameraTimeout": 20000, "TimeFirst": false }, "640 AO-Name": "AnalogIO", "Vincent-D3-Command": "Close" }, "FrameKey-0-0-1": { "COM4-StopBits": "1", "528 AO-MaxVolts": "5.0000", "XLIGHT Excitation Wheel-State": "1", "470 Shutter-Name": "AnalogIO", "Core-Shutter": "520", "555 AO-Demo": "No", "XLIGHT Dichroic-State": "2", "XYStage-StepSize-Y": "0.1000", "ZPositionUm": 178.48000000000002, "XYStage-StepSize-X": "0.1000", "PRIME-FirmwareVersion": "20.28", "PRIME-ClearCycles": "2", "520-Name": "DA Shutter", "405 Shutter-Name": "AnalogIO", "PIEZO-StepSize": "0.0050", "PRIME-ClearMode": "Pre-Exposure", "470 AO-MinVolts": "0.0000", "405 AO-SupportsTriggering": "1", "CameraChannelIndex": 0, "XYStage-StartSpeed": "500.0000", "COM9-StopBits": "2", "405 AO-Description": "NI DAC", "COM4-DelayBetweenCharsMs": "0.0000", "445 AO-Block voltage": "false", "520 AO-TriggerInputLine": "/Dev1/PFI0", "555 AO-Description": "NI DAC", "520 AO-TriggerSampleRate": "100000", "COM4-Verbose": "1", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM4-Name": "COM4", "405 Shutter-Volts": "0.0000", "Vincent-D3-Port": "COM1", "PRIME-PP 4 MIN ADU AFFECTED": "200", "470 Shutter-MinVolts": "0.0000", "PRIME-PP 3 ENABLED": "Yes", "470 AO-Description": "NI DAC", "PRIME-Description": "PVCAM API device adapter", "520 Shutter-MaxVolts": "5.0000", "520 Shutter-Volts": "5.0000", "528 AO-Sequenceable": "1", "PRIME-Binning": "1x1", "555 Shutter-MaxVolts": "5.0000", "528 Shutter-TriggerSequenceLength": "1000", "405 Shutter-Block voltage": "false", "XLIGHT Spin motor-State": "1", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "Vincent-D3-Time to open (ms)": "35.0000", "Vincent-D3-Time to close (ms)": "35.0000", "445 AO-TriggerSampleRate": "100000", "640 Shutter-IOChannel": "Dev1/ao14", "555 AO-Sequenceable": "1", "520 AO-Name": "AnalogIO", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "470 AO-Block voltage": "false", "COM1-Verbose": "1", "COM9-AnswerTimeout": "10000.0000", "528 Shutter-Name": "AnalogIO", "PRIME-Trigger Timeout (secs)": "10", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "NextFrame": 0, "470 AO-TriggerSequenceLength": "1000", "PRIME-TransposeXY": "0", "445 AO-TriggerSequenceLength": "1000", "COM1-Name": "COM1", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "COM9-DataBits": "8", "COM1-AnswerTimeout": "500.0000", "Camera": "", "TE2000-Version": "V2.50-V2.50-V2.10", "528 Shutter-Volts": "0.0000", "XLIGHT Hub-Name": "XLIGHT Hub", "PIEZO-Description": "Ludl stage driver adapter", "PositionName": "Pos29", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSampleRate": "100000", "PRIME-FanSpeedSetpoint": "High", "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "405 AO-TriggerSampleRate": "100000", "470 Shutter-Block voltage": "false", "470-State": "0", "COM3-Parity": "None", "405 AO-Volts": "0.0000", "PRIME-TransposeMirrorY": "0", "PRIME-ChipName": "GS144BSI", "470 AO-Volts": "0.0000", "PRIME-TransposeMirrorX": "0", "445 Shutter-SupportsTriggering": "0", "470 Shutter-SupportsTriggering": "0", "XLIGHT Hub-Description": "XLIGHT hub", "PRIME-PreampOffLimit": "10000", "470 AO-Sequenceable": "1", "528 Shutter-MaxVolts": "5.0000", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "Core-XYStage": "XYStage", "470 AO-TriggerSampleRate": "100000", "PRIME-Timing-PreTriggerDelayNs": "24672000.0000", "528-DA Device": "528 Shutter", "Focus-Description": "Nikon TE2000 focus adapter", "FilterBlock-State": "5", "520 AO-Demo": "No", "COM3-DelayBetweenCharsMs": "0.0000", "470 Shutter-Volts": "0.0000", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "PRIME-PP 2 THRESHOLD": "125", "PRIME-PP 4 THRESHOLD": "75", "XYStage-Acceleration": "4.0000", "470 Shutter-Demo": "No", "PRIME-Name": "PMPCIECam00", "640-DA Device": "640 Shutter", "405 AO-Block voltage": "false", "ElapsedTime-ms": 1190831, "PRIME-Offset": "100", "445 AO-IOChannel": "Dev1/ao1", "640 AO-MaxVolts": "5.0000", "PositionIndex": 29, "PRIME-PP 1 ENABLED": "Yes", "COM1-BaudRate": "9600", "640-State": "0", "COM3-Verbose": "1", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "LudlController-TransmissionDelay": "0", "PRIME-FullWellCapacity": "62000", "PRIME-SMARTStreamingEnabled": "No", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "640 Shutter-Description": "NI DAC", "528-Description": "DA device that is used as a shutter", "COM4-Description": "Serial port driver (boost:asio)", "405 Shutter-Description": "NI DAC", "TE2000-Name": "TE2000", "528 Shutter-Description": "NI DAC", "COM9-Description": "Serial port driver (boost:asio)", "LudlController-Name": "LudlController", "445 Shutter-TriggerSequenceLength": "1000", "PRIME-SerialNumber": "A18B203008", "445 AO-Volts": "0.0000", "PRIME-X-dimension": "1200", "PRIME-ShutterCloseDelay": "0", "XLIGHT Disk slider-State": "2", "Position": "Default", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 AO-IOChannel": "Dev1/ao5", "520 AO-IOChannel": "Dev1/ao4", "528 AO-TriggerSequenceLength": "1000", "405 Shutter-MaxVolts": "5.0000", "470-DA Device": "470 Shutter", "PRIME-FTCapable": "No", "Core-ChannelGroup": "Channels", "555 AO-SupportsTriggering": "0", "PRIME-Y-dimension": "1200", "Frame": 0, "555 Shutter-Sequenceable": "1", "PRIME-Timing-ExposureTimeNs": "499998000.0000", "Core-ImageProcessor": "", "528-State": "0", "520 AO-Block voltage": "false", "XLIGHT Excitation Wheel-Label": "CFP/YFP", "640 Shutter-Demo": "No", "COM3-StopBits": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "520 Shutter-IOChannel": "Dev1/ao11", "Width": 1200, "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-SupportsTriggering": "0", "640 AO-TriggerSampleRate": "100000", "520-Description": "DA device that is used as a shutter", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "405 Shutter-Sequenceable": "1", "405 Shutter-SupportsTriggering": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-TriggerSampleRate": "100000", "640 Shutter-MaxVolts": "5.0000", "555 AO-MaxVolts": "5.0000", "520 AO-TriggerSequenceLength": "1000", "COM3-BaudRate": "9600", "COM9-BaudRate": "9600", "Vincent-D3-Channel #": "Ch. #1", "PRIME-PMode": "Normal", "OpticalPath-Label": "Left(100)", "BitDepth": 16, "555 AO-TriggerSequenceLength": "1000", "445 Shutter-Description": "NI DAC", "405 AO-Sequenceable": "1", "PRIME-AcquisitionMethod": "Callbacks", "555 AO-Name": "AnalogIO", "PRIME-ShutterMode": "Pre-Exposure", "COM3-Handshaking": "Off", "445 AO-SupportsTriggering": "0", "ROI": "0-0-1200-1200", "COM9-Verbose": "1", "PRIME-Color": "OFF", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "PRIME-ShutterOpenDelay": "0", "COM1-Parity": "None", "Slice": 1, "SlicePosition": -40, "COM9-DelayBetweenCharsMs": "0.0000", "555-Name": "DA Shutter", "PIEZO-LudlSingleAxisName": "B", "XLIGHT Emission Wheel-Label": "565/24(G)", "640 Shutter-Name": "AnalogIO", "640 AO-Sequenceable": "1", "520 Shutter-Demo": "No", "528 Shutter-Demo": "No", "470-Name": "DA Shutter", "520-DA Device": "520 Shutter", "XYStage-Description": "Ludl XY stage driver adapter", "445 Shutter-MinVolts": "0.0000", "528 AO-TriggerSampleRate": "100000", "405 AO-MinVolts": "0.0000", "405 Shutter-TriggerSequenceLength": "1000", "Height": 1200, "555 AO-Block voltage": "false", "640 Shutter-Volts": "0.0000", "PixelType": "GRAY16", "445 Shutter-Block voltage": "false", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "520 Shutter-TriggerSequenceLength": "1000", "COM9-Name": "COM9", "Core-SLM": "", "555 AO-Volts": "0.0000", "555 Shutter-Block voltage": "false", "XYStage-Speed": "1800.0000", "520 AO-MaxVolts": "5.0000", "528 Shutter-SupportsTriggering": "0", "COM1-Description": "Serial port driver (boost:asio)", "PRIME-MetadataEnabled": "No", "520 Shutter-MinVolts": "0.0000", "470 Shutter-Sequenceable": "1", "PRIME-PreampDelay": "0", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-MinVolts": "0.0000", "ChannelIndex": 0, "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Core-AutoShutter": "1", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "Exposure-ms": 320, "PRIME-PP 2 ENABLED": "Yes", "640 Shutter-TriggerSampleRate": "100000", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "470 Shutter-IOChannel": "Dev1/ao10", "405 AO-Demo": "No", "PixelSizeUm": 1.1, "470 AO-Demo": "No", "470 Shutter-MaxVolts": "5.0000", "Vincent-D3-Name": "Vincent-D3", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FileName": "img_000000000_Il-G_001.tif", "LudlController-Reset": "Operate", "PRIME-Exposure": "320.00", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "445-Name": "DA Shutter", "555 Shutter-Name": "AnalogIO", "520 Shutter-TriggerSampleRate": "100000", "445 Shutter-IOChannel": "Dev1/ao9", "PRIME-TransposeCorrection": "0", "XYStage-StepSize": "0.1000", "445-Description": "DA device that is used as a shutter", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 AO-IOChannel": "Dev1/ao7", "520 Shutter-Name": "AnalogIO", "COM3-DataBits": "8", "640 AO-Volts": "0.0000", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "555 Shutter-IOChannel": "Dev1/ao13", "WaitInterval": null, "520 Shutter-Sequenceable": "1", "YPositionUm": -180, "445-State": "0", "445-DA Device": "445 Shutter", "PRIME-CircularBufferAutoSize": "ON", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "555 AO-TriggerSampleRate": "100000", "445 Shutter-Volts": "0.0000", "XLIGHT Hub-Port": "COM3", "Core-AutoFocus": "", "COM4-Handshaking": "Off", "528 Shutter-MinVolts": "0.0000", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "445 Shutter-Name": "AnalogIO", "528 Shutter-Sequenceable": "1", "XYStage-TransposeMirrorX": "0", "XYStage-TransposeMirrorY": "0", "PRIME-ReadoutRate": "100MHz 16bit", "XYStage-Name": "Stage", "640 AO-SupportsTriggering": "0", "640-Name": "DA Shutter", "640 Shutter-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "640 AO-Block voltage": "false", "445 Shutter-Sequenceable": "1", "470 AO-MaxVolts": "5.0000", "COM4-BaudRate": "9600", "470-Description": "DA device that is used as a shutter", "520 Shutter-Description": "NI DAC", "PRIME-CCDTemperature": "-14.8500", "Time": "2019-12-18 23:10:51 -0500", "640 AO-TriggerSequenceLength": "1000", "470 AO-Name": "AnalogIO", "XLIGHT Disk slider-Label": "Disk Pos 40um", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "Binning": "1x1", "TE2000-ModelType": "1", "COM3-AnswerTimeout": "500.0000", "555 Shutter-SupportsTriggering": "0", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "TE2000-Port": "COM4", "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Description": "NI DAC", "COM4-AnswerTimeout": "500.0000", "COM1-DelayBetweenCharsMs": "0.0000", "FilterBlock-Label": "Confocal", "445 AO-Name": "AnalogIO", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-Timing-ReadoutTimeNs": "24672000.0000", "528 AO-Demo": "No", "520 AO-Sequenceable": "1", "555-Description": "DA device that is used as a shutter", "405-DA Device": "405 Shutter", "405 AO-TriggerSequenceLength": "1000", "640 AO-MinVolts": "0.0000", "640 Shutter-Block voltage": "false", "FrameIndex": 0, "PRIME-Port": "CMOS", "555 AO-MinVolts": "0.0000", "COM3-Description": "Serial port driver (boost:asio)", "528 AO-Description": "NI DAC", "555 AO-IOChannel": "Dev1/ao6", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "555 AO-TriggerInputLine": "/Dev1/PFI0", "PRIME-ActualInterval-ms": "390.5200", "LudlController-Port": "COM9", "445 Shutter-Demo": "No", "PRIME-Trigger-Expose Out-Mux": "1", "SliceIndex": 1, "PRIME-CCDTemperatureSetPoint": "-15.0000", "405 Shutter-MinVolts": "0.0000", "PRIME-ExposeOutMode": "First Row", "AxisPositions": null, "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Volts": "0.0000", "528-Name": "DA Shutter", "555-State": "0", "XLIGHT Dichroic-Label": "CFP/YFP", "445 AO-Sequenceable": "1", "405-Name": "DA Shutter", "405 AO-IOChannel": "Dev1/ao0", "Core-Camera": "PRIME", "Focus-Name": "Focus", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "405 Shutter-IOChannel": "Dev1/ao8", "640 AO-Demo": "No", "640 Shutter-SupportsTriggering": "0", "445 AO-Demo": "No", "555-DA Device": "555 Shutter", "470 Shutter-Description": "NI DAC", "528 AO-Volts": "0.0000", "COM1-DataBits": "8", "Vincent-D3-Address": "x", "520 AO-SupportsTriggering": "0", "405-Description": "DA device that is used as a shutter", "640 Shutter-MinVolts": "0.0000", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "405 AO-Name": "AnalogIO", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "640 AO-TriggerInputLine": "/Dev1/PFI0", "405 AO-TriggerInputLine": "/Dev1/PFI0", "640-Description": "DA device that is used as a shutter", "445 Shutter-TriggerSampleRate": "100000", "470 Shutter-TriggerSequenceLength": "1000", "COM1-StopBits": "1", "520 Shutter-SupportsTriggering": "0", "405-State": "0", "Core-Initialize": "1", "XLIGHT Emission Wheel-State": "3", "520 AO-Description": "NI DAC", "528 AO-TriggerInputLine": "/Dev1/PFI0", "445 Shutter-MaxVolts": "5.0000", "COM9-Handshaking": "Off", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "XLIGHT Spin motor-Label": "On (spinning)", "PRIME-PP 5 ENABLED": "No", "UUID": "a3121f8c-4ca2-4a4a-833c-ba58b1da90a3", "PRIME-TriggerMode": "Internal Trigger", "COM1-Handshaking": "Off", "PRIME-Timing-PostTriggerDelayNs": "49344000.0000", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "Focus-Position": "17741.5000", "528 Shutter-Block voltage": "false", "Channel": "Il-G", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "555 Shutter-Demo": "No", "405 Shutter-Demo": "No", "528 AO-Name": "AnalogIO", "520-State": "1", "Core-Galvo": "", "405 Shutter-TriggerSampleRate": "100000", "Focus-FocusStepSize_(nm)": "100", "PRIME-PixelType": "16bit", "COM4-DataBits": "8", "470 AO-IOChannel": "Dev1/ao2", "520 AO-MinVolts": "0.0000", "COM9-Parity": "None", "PRIME-CircularBufferEnabled": "ON", "PRIME-PP 6 Reset": "No", "640 AO-Description": "NI DAC", "Source": "PRIME", "445 AO-MinVolts": "0.0000", "PIEZO-Name": "Stage", "445 AO-Description": "NI DAC", "PRIME-CircularBufferFrameCount": "46", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "520 AO-Volts": "4.5000", "528 Shutter-TriggerSampleRate": "100000", "XPositionUm": 15712.1, "OpticalPath-State": "4", "PRIME-PP 3 THRESHOLD": "80", "PRIME-PVCAM Version": "3.7.1", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Gain": "1-HDR", "640 Shutter-TriggerSequenceLength": "1000", "528 AO-SupportsTriggering": "0", "445 AO-TriggerInputLine": "/Dev1/PFI0", "OpticalPath-Name": "OpticalPath", "PRIME-PP 4 ENABLED": "Yes", "PRIME-PVCAM Adapter Version": "1.3.29", "COM3-Name": "COM3", "405 AO-MaxVolts": "5.0000", "520 Shutter-Block voltage": "false", "PRIME-Timing-ClearingTimeNs": "49344000.0000", "Core-Focus": "PIEZO", "528 AO-Block voltage": "false", "TE2000-Description": "Nikon TE2000 hub", "528 AO-MinVolts": "0.0000", "COM4-Parity": "None", "Summary": { "Slices": 11, "ChContrastMin": [ 0, 0, 0, 0, 0, 0 ], "PixelAspect": 1, "SlicesFirst": true, "Interval_ms": 0, "Time": "2019-12-18 23:10:50 -0500", "Prefix": "seq_5", "InitialPositionList": [ { "Label": "Pos0", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.21], "Focus": [17741.5], "XYStage": [ 44542.200000000004, 31636.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos1", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.22], "Focus": [17741.4], "XYStage": [ 43801.100000000006, 31637.9 ] }, "GridColumnIndex": 0 }, { "Label": "Pos2", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.5], "XYStage": [ 38561.3, 32040.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos3", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.185], "Focus": [17741.3], "XYStage": [ 37698.4, 32041.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos4", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.16], "Focus": [17741.5], "XYStage": [ 32375.4, 31758.9 ] }, "GridColumnIndex": 0 }, { "Label": "Pos5", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.17000000000002], "Focus": [17741.3], "XYStage": [ 31770.7, 31758.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos6", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.4], "XYStage": [ 26536.800000000003, 31068.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos7", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.205], "Focus": [17741.4], "XYStage": [ 25420.2, 31068.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos8", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.85], "Focus": [17741.3], "XYStage": [ 21059, 31508 ] }, "GridColumnIndex": 0 }, { "Label": "Pos9", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.82], "Focus": [17741.3], "XYStage": [ 19731.9, 31506.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos10", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.79], "Focus": [17741.4], "XYStage": [ 14528.5, 32007.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos11", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.83], "Focus": [17741.2], "XYStage": [ 13830.300000000001, 32009.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos12", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.79], "Focus": [17741.3], "XYStage": [ 8775.300000000001, 32017.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos13", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.815], "Focus": [17741.3], "XYStage": [ 8109.200000000001, 32008.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos14", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.805], "Focus": [17741.3], "XYStage": [ 3483.3, 31908.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos15", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.82], "Focus": [17741.2], "XYStage": [ 2686.8, 31903.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos16", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [211.48000000000002], "Focus": [17741.1], "XYStage": [ -1978.9, 31973.600000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos17", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [211.51500000000001], "Focus": [17741.1], "XYStage": [ -2957.8, 31982.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos18", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.195], "Focus": [17741.5], "XYStage": [ 47747.4, -576.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos19", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.23000000000002], "Focus": [17741.6], "XYStage": [ 47053.9, -576.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos20", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.205], "Focus": [17741.4], "XYStage": [ 41871.200000000004, -479.8 ] }, "GridColumnIndex": 0 }, { "Label": "Pos21", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.4], "XYStage": [ 40882.600000000006, -392 ] }, "GridColumnIndex": 0 }, { "Label": "Pos22", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.24], "Focus": [17741.4], "XYStage": [ 35905.6, -135.3 ] }, "GridColumnIndex": 0 }, { "Label": "Pos23", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.195], "Focus": [17741.5], "XYStage": [ 34990.9, -132.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos24", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.46], "Focus": [17741.4], "XYStage": [ 29678.100000000002, -958.1 ] }, "GridColumnIndex": 0 }, { "Label": "Pos25", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.44], "Focus": [17741.5], "XYStage": [ 28983.600000000002, -939.4000000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos26", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.475], "Focus": [17741.5], "XYStage": [ 23019.7, -122 ] }, "GridColumnIndex": 0 }, { "Label": "Pos27", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.44], "Focus": [17741.7], "XYStage": [ 22428, -116.80000000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos28", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.46], "Focus": [17741.7], "XYStage": [ 16364.900000000001, -181.10000000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos29", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.48000000000002], "Focus": [17741.5], "XYStage": [ 15712.1, -180 ] }, "GridColumnIndex": 0 }, { "Label": "Pos30", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.45000000000002], "Focus": [17741.7], "XYStage": [ 10346.1, 77.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos31", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.49], "Focus": [17741.4], "XYStage": [ 9755.5, 85.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos32", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.475], "Focus": [17741.5], "XYStage": [ 4775.5, 293.90000000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos33", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.445], "Focus": [17741.4], "XYStage": [ 3954.9, 293.90000000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos34", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.455], "Focus": [17741.4], "XYStage": [ -1324.9, 545.6 ] }, "GridColumnIndex": 0 }, { "Label": "Pos35", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.445], "Focus": [17741.3], "XYStage": [ -2097, 576.6 ] }, "GridColumnIndex": 0 } ], "z-step_um": 10, "GridRow": 0, "BitDepth": 16, "Depth": 2, "Channels": 6, "Source": "Micro-Manager", "ChColors": [ -16711681, -256, -65281, -1, -1, -16776961 ], "MetadataVersion": 10, "KeepShutterOpenChannels": true, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536, 65536 ], "CustomIntervals_ms": [], "Height": 1200, "MicroManagerVersion": "1.4.23 20180219", "GridColumn": 0, "UUID": "67598d21-5db5-4842-ab60-f506f7b42c7c", "PixelType": "GRAY16", "Width": 1200, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\Yan\\20191218-YC113-S7S8-miseq", "Frames": 1, "Comment": "", "UserName": "Nikon", "PixelSize_um": 1.1, "ROI": [ 0, 0, 1200, 1200 ], "Date": "2019-12-18", "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC", "P-DAPI-high" ], "KeepShutterOpenSlices": true, "IJType": 1, "ComputerName": "ZadorConfocal2", "Positions": 36, "PositionIndex": 29, "CameraTimeout": 20000, "TimeFirst": false }, "640 AO-Name": "AnalogIO", "Vincent-D3-Command": "Close" }, "FrameKey-0-0-2": { "COM4-StopBits": "1", "528 AO-MaxVolts": "5.0000", "XLIGHT Excitation Wheel-State": "1", "470 Shutter-Name": "AnalogIO", "Core-Shutter": "520", "555 AO-Demo": "No", "XLIGHT Dichroic-State": "2", "XYStage-StepSize-Y": "0.1000", "ZPositionUm": 188.48000000000002, "XYStage-StepSize-X": "0.1000", "PRIME-FirmwareVersion": "20.28", "PRIME-ClearCycles": "2", "520-Name": "DA Shutter", "405 Shutter-Name": "AnalogIO", "PIEZO-StepSize": "0.0050", "PRIME-ClearMode": "Pre-Exposure", "470 AO-MinVolts": "0.0000", "405 AO-SupportsTriggering": "1", "CameraChannelIndex": 0, "XYStage-StartSpeed": "500.0000", "COM9-StopBits": "2", "405 AO-Description": "NI DAC", "COM4-DelayBetweenCharsMs": "0.0000", "445 AO-Block voltage": "false", "520 AO-TriggerInputLine": "/Dev1/PFI0", "555 AO-Description": "NI DAC", "520 AO-TriggerSampleRate": "100000", "COM4-Verbose": "1", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM4-Name": "COM4", "405 Shutter-Volts": "0.0000", "Vincent-D3-Port": "COM1", "PRIME-PP 4 MIN ADU AFFECTED": "200", "470 Shutter-MinVolts": "0.0000", "PRIME-PP 3 ENABLED": "Yes", "470 AO-Description": "NI DAC", "PRIME-Description": "PVCAM API device adapter", "520 Shutter-MaxVolts": "5.0000", "520 Shutter-Volts": "5.0000", "528 AO-Sequenceable": "1", "PRIME-Binning": "1x1", "555 Shutter-MaxVolts": "5.0000", "528 Shutter-TriggerSequenceLength": "1000", "405 Shutter-Block voltage": "false", "XLIGHT Spin motor-State": "1", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "Vincent-D3-Time to open (ms)": "35.0000", "Vincent-D3-Time to close (ms)": "35.0000", "445 AO-TriggerSampleRate": "100000", "640 Shutter-IOChannel": "Dev1/ao14", "555 AO-Sequenceable": "1", "520 AO-Name": "AnalogIO", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "470 AO-Block voltage": "false", "COM1-Verbose": "1", "COM9-AnswerTimeout": "10000.0000", "528 Shutter-Name": "AnalogIO", "PRIME-Trigger Timeout (secs)": "10", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "NextFrame": 0, "470 AO-TriggerSequenceLength": "1000", "PRIME-TransposeXY": "0", "445 AO-TriggerSequenceLength": "1000", "COM1-Name": "COM1", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "COM9-DataBits": "8", "COM1-AnswerTimeout": "500.0000", "Camera": "", "TE2000-Version": "V2.50-V2.50-V2.10", "528 Shutter-Volts": "0.0000", "XLIGHT Hub-Name": "XLIGHT Hub", "PIEZO-Description": "Ludl stage driver adapter", "PositionName": "Pos29", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSampleRate": "100000", "PRIME-FanSpeedSetpoint": "High", "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "405 AO-TriggerSampleRate": "100000", "470 Shutter-Block voltage": "false", "470-State": "0", "COM3-Parity": "None", "405 AO-Volts": "0.0000", "PRIME-TransposeMirrorY": "0", "PRIME-ChipName": "GS144BSI", "470 AO-Volts": "0.0000", "PRIME-TransposeMirrorX": "0", "445 Shutter-SupportsTriggering": "0", "470 Shutter-SupportsTriggering": "0", "XLIGHT Hub-Description": "XLIGHT hub", "PRIME-PreampOffLimit": "10000", "470 AO-Sequenceable": "1", "528 Shutter-MaxVolts": "5.0000", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "Core-XYStage": "XYStage", "470 AO-TriggerSampleRate": "100000", "PRIME-Timing-PreTriggerDelayNs": "24672000.0000", "528-DA Device": "528 Shutter", "Focus-Description": "Nikon TE2000 focus adapter", "FilterBlock-State": "5", "520 AO-Demo": "No", "COM3-DelayBetweenCharsMs": "0.0000", "470 Shutter-Volts": "0.0000", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "PRIME-PP 2 THRESHOLD": "125", "PRIME-PP 4 THRESHOLD": "75", "XYStage-Acceleration": "4.0000", "470 Shutter-Demo": "No", "PRIME-Name": "PMPCIECam00", "640-DA Device": "640 Shutter", "405 AO-Block voltage": "false", "ElapsedTime-ms": 1191438, "PRIME-Offset": "100", "445 AO-IOChannel": "Dev1/ao1", "640 AO-MaxVolts": "5.0000", "PositionIndex": 29, "PRIME-PP 1 ENABLED": "Yes", "COM1-BaudRate": "9600", "640-State": "0", "COM3-Verbose": "1", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "LudlController-TransmissionDelay": "0", "PRIME-FullWellCapacity": "62000", "PRIME-SMARTStreamingEnabled": "No", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "640 Shutter-Description": "NI DAC", "528-Description": "DA device that is used as a shutter", "COM4-Description": "Serial port driver (boost:asio)", "405 Shutter-Description": "NI DAC", "TE2000-Name": "TE2000", "528 Shutter-Description": "NI DAC", "COM9-Description": "Serial port driver (boost:asio)", "LudlController-Name": "LudlController", "445 Shutter-TriggerSequenceLength": "1000", "PRIME-SerialNumber": "A18B203008", "445 AO-Volts": "0.0000", "PRIME-X-dimension": "1200", "PRIME-ShutterCloseDelay": "0", "XLIGHT Disk slider-State": "2", "Position": "Default", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 AO-IOChannel": "Dev1/ao5", "520 AO-IOChannel": "Dev1/ao4", "528 AO-TriggerSequenceLength": "1000", "405 Shutter-MaxVolts": "5.0000", "470-DA Device": "470 Shutter", "PRIME-FTCapable": "No", "Core-ChannelGroup": "Channels", "555 AO-SupportsTriggering": "0", "PRIME-Y-dimension": "1200", "Frame": 0, "555 Shutter-Sequenceable": "1", "PRIME-Timing-ExposureTimeNs": "499998000.0000", "Core-ImageProcessor": "", "528-State": "0", "520 AO-Block voltage": "false", "XLIGHT Excitation Wheel-Label": "CFP/YFP", "640 Shutter-Demo": "No", "COM3-StopBits": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "520 Shutter-IOChannel": "Dev1/ao11", "Width": 1200, "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-SupportsTriggering": "0", "640 AO-TriggerSampleRate": "100000", "520-Description": "DA device that is used as a shutter", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "405 Shutter-Sequenceable": "1", "405 Shutter-SupportsTriggering": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-TriggerSampleRate": "100000", "640 Shutter-MaxVolts": "5.0000", "555 AO-MaxVolts": "5.0000", "520 AO-TriggerSequenceLength": "1000", "COM3-BaudRate": "9600", "COM9-BaudRate": "9600", "Vincent-D3-Channel #": "Ch. #1", "PRIME-PMode": "Normal", "OpticalPath-Label": "Left(100)", "BitDepth": 16, "555 AO-TriggerSequenceLength": "1000", "445 Shutter-Description": "NI DAC", "405 AO-Sequenceable": "1", "PRIME-AcquisitionMethod": "Callbacks", "555 AO-Name": "AnalogIO", "PRIME-ShutterMode": "Pre-Exposure", "COM3-Handshaking": "Off", "445 AO-SupportsTriggering": "0", "ROI": "0-0-1200-1200", "COM9-Verbose": "1", "PRIME-Color": "OFF", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "PRIME-ShutterOpenDelay": "0", "COM1-Parity": "None", "Slice": 2, "SlicePosition": -30, "COM9-DelayBetweenCharsMs": "0.0000", "555-Name": "DA Shutter", "PIEZO-LudlSingleAxisName": "B", "XLIGHT Emission Wheel-Label": "565/24(G)", "640 Shutter-Name": "AnalogIO", "640 AO-Sequenceable": "1", "520 Shutter-Demo": "No", "528 Shutter-Demo": "No", "470-Name": "DA Shutter", "520-DA Device": "520 Shutter", "XYStage-Description": "Ludl XY stage driver adapter", "445 Shutter-MinVolts": "0.0000", "528 AO-TriggerSampleRate": "100000", "405 AO-MinVolts": "0.0000", "405 Shutter-TriggerSequenceLength": "1000", "Height": 1200, "555 AO-Block voltage": "false", "640 Shutter-Volts": "0.0000", "PixelType": "GRAY16", "445 Shutter-Block voltage": "false", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "520 Shutter-TriggerSequenceLength": "1000", "COM9-Name": "COM9", "Core-SLM": "", "555 AO-Volts": "0.0000", "555 Shutter-Block voltage": "false", "XYStage-Speed": "1800.0000", "520 AO-MaxVolts": "5.0000", "528 Shutter-SupportsTriggering": "0", "COM1-Description": "Serial port driver (boost:asio)", "PRIME-MetadataEnabled": "No", "520 Shutter-MinVolts": "0.0000", "470 Shutter-Sequenceable": "1", "PRIME-PreampDelay": "0", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-MinVolts": "0.0000", "ChannelIndex": 0, "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Core-AutoShutter": "1", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "Exposure-ms": 320, "PRIME-PP 2 ENABLED": "Yes", "640 Shutter-TriggerSampleRate": "100000", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "470 Shutter-IOChannel": "Dev1/ao10", "405 AO-Demo": "No", "PixelSizeUm": 1.1, "470 AO-Demo": "No", "470 Shutter-MaxVolts": "5.0000", "Vincent-D3-Name": "Vincent-D3", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FileName": "img_000000000_Il-G_002.tif", "LudlController-Reset": "Operate", "PRIME-Exposure": "320.00", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "445-Name": "DA Shutter", "555 Shutter-Name": "AnalogIO", "520 Shutter-TriggerSampleRate": "100000", "445 Shutter-IOChannel": "Dev1/ao9", "PRIME-TransposeCorrection": "0", "XYStage-StepSize": "0.1000", "445-Description": "DA device that is used as a shutter", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 AO-IOChannel": "Dev1/ao7", "520 Shutter-Name": "AnalogIO", "COM3-DataBits": "8", "640 AO-Volts": "0.0000", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "555 Shutter-IOChannel": "Dev1/ao13", "WaitInterval": null, "520 Shutter-Sequenceable": "1", "YPositionUm": -180, "445-State": "0", "445-DA Device": "445 Shutter", "PRIME-CircularBufferAutoSize": "ON", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "555 AO-TriggerSampleRate": "100000", "445 Shutter-Volts": "0.0000", "XLIGHT Hub-Port": "COM3", "Core-AutoFocus": "", "COM4-Handshaking": "Off", "528 Shutter-MinVolts": "0.0000", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "445 Shutter-Name": "AnalogIO", "528 Shutter-Sequenceable": "1", "XYStage-TransposeMirrorX": "0", "XYStage-TransposeMirrorY": "0", "PRIME-ReadoutRate": "100MHz 16bit", "XYStage-Name": "Stage", "640 AO-SupportsTriggering": "0", "640-Name": "DA Shutter", "640 Shutter-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "640 AO-Block voltage": "false", "445 Shutter-Sequenceable": "1", "470 AO-MaxVolts": "5.0000", "COM4-BaudRate": "9600", "470-Description": "DA device that is used as a shutter", "520 Shutter-Description": "NI DAC", "PRIME-CCDTemperature": "-14.8500", "Time": "2019-12-18 23:10:51 -0500", "640 AO-TriggerSequenceLength": "1000", "470 AO-Name": "AnalogIO", "XLIGHT Disk slider-Label": "Disk Pos 40um", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "Binning": "1x1", "TE2000-ModelType": "1", "COM3-AnswerTimeout": "500.0000", "555 Shutter-SupportsTriggering": "0", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "TE2000-Port": "COM4", "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Description": "NI DAC", "COM4-AnswerTimeout": "500.0000", "COM1-DelayBetweenCharsMs": "0.0000", "FilterBlock-Label": "Confocal", "445 AO-Name": "AnalogIO", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-Timing-ReadoutTimeNs": "24672000.0000", "528 AO-Demo": "No", "520 AO-Sequenceable": "1", "555-Description": "DA device that is used as a shutter", "405-DA Device": "405 Shutter", "405 AO-TriggerSequenceLength": "1000", "640 AO-MinVolts": "0.0000", "640 Shutter-Block voltage": "false", "FrameIndex": 0, "PRIME-Port": "CMOS", "555 AO-MinVolts": "0.0000", "COM3-Description": "Serial port driver (boost:asio)", "528 AO-Description": "NI DAC", "555 AO-IOChannel": "Dev1/ao6", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "555 AO-TriggerInputLine": "/Dev1/PFI0", "PRIME-ActualInterval-ms": "390.5200", "LudlController-Port": "COM9", "445 Shutter-Demo": "No", "PRIME-Trigger-Expose Out-Mux": "1", "SliceIndex": 2, "PRIME-CCDTemperatureSetPoint": "-15.0000", "405 Shutter-MinVolts": "0.0000", "PRIME-ExposeOutMode": "First Row", "AxisPositions": null, "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Volts": "0.0000", "528-Name": "DA Shutter", "555-State": "0", "XLIGHT Dichroic-Label": "CFP/YFP", "445 AO-Sequenceable": "1", "405-Name": "DA Shutter", "405 AO-IOChannel": "Dev1/ao0", "Core-Camera": "PRIME", "Focus-Name": "Focus", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "405 Shutter-IOChannel": "Dev1/ao8", "640 AO-Demo": "No", "640 Shutter-SupportsTriggering": "0", "445 AO-Demo": "No", "555-DA Device": "555 Shutter", "470 Shutter-Description": "NI DAC", "528 AO-Volts": "0.0000", "COM1-DataBits": "8", "Vincent-D3-Address": "x", "520 AO-SupportsTriggering": "0", "405-Description": "DA device that is used as a shutter", "640 Shutter-MinVolts": "0.0000", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "405 AO-Name": "AnalogIO", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "640 AO-TriggerInputLine": "/Dev1/PFI0", "405 AO-TriggerInputLine": "/Dev1/PFI0", "640-Description": "DA device that is used as a shutter", "445 Shutter-TriggerSampleRate": "100000", "470 Shutter-TriggerSequenceLength": "1000", "COM1-StopBits": "1", "520 Shutter-SupportsTriggering": "0", "405-State": "0", "Core-Initialize": "1", "XLIGHT Emission Wheel-State": "3", "520 AO-Description": "NI DAC", "528 AO-TriggerInputLine": "/Dev1/PFI0", "445 Shutter-MaxVolts": "5.0000", "COM9-Handshaking": "Off", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "XLIGHT Spin motor-Label": "On (spinning)", "PRIME-PP 5 ENABLED": "No", "UUID": "410a79a4-3df6-4bda-a374-2ab518db1aac", "PRIME-TriggerMode": "Internal Trigger", "COM1-Handshaking": "Off", "PRIME-Timing-PostTriggerDelayNs": "49344000.0000", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "Focus-Position": "17741.5000", "528 Shutter-Block voltage": "false", "Channel": "Il-G", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "555 Shutter-Demo": "No", "405 Shutter-Demo": "No", "528 AO-Name": "AnalogIO", "520-State": "1", "Core-Galvo": "", "405 Shutter-TriggerSampleRate": "100000", "Focus-FocusStepSize_(nm)": "100", "PRIME-PixelType": "16bit", "COM4-DataBits": "8", "470 AO-IOChannel": "Dev1/ao2", "520 AO-MinVolts": "0.0000", "COM9-Parity": "None", "PRIME-CircularBufferEnabled": "ON", "PRIME-PP 6 Reset": "No", "640 AO-Description": "NI DAC", "Source": "PRIME", "445 AO-MinVolts": "0.0000", "PIEZO-Name": "Stage", "445 AO-Description": "NI DAC", "PRIME-CircularBufferFrameCount": "46", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "520 AO-Volts": "4.5000", "528 Shutter-TriggerSampleRate": "100000", "XPositionUm": 15712.1, "OpticalPath-State": "4", "PRIME-PP 3 THRESHOLD": "80", "PRIME-PVCAM Version": "3.7.1", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Gain": "1-HDR", "640 Shutter-TriggerSequenceLength": "1000", "528 AO-SupportsTriggering": "0", "445 AO-TriggerInputLine": "/Dev1/PFI0", "OpticalPath-Name": "OpticalPath", "PRIME-PP 4 ENABLED": "Yes", "PRIME-PVCAM Adapter Version": "1.3.29", "COM3-Name": "COM3", "405 AO-MaxVolts": "5.0000", "520 Shutter-Block voltage": "false", "PRIME-Timing-ClearingTimeNs": "49344000.0000", "Core-Focus": "PIEZO", "528 AO-Block voltage": "false", "TE2000-Description": "Nikon TE2000 hub", "528 AO-MinVolts": "0.0000", "COM4-Parity": "None", "Summary": { "Slices": 11, "ChContrastMin": [ 0, 0, 0, 0, 0, 0 ], "PixelAspect": 1, "SlicesFirst": true, "Interval_ms": 0, "Time": "2019-12-18 23:10:50 -0500", "Prefix": "seq_5", "InitialPositionList": [ { "Label": "Pos0", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.21], "Focus": [17741.5], "XYStage": [ 44542.200000000004, 31636.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos1", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.22], "Focus": [17741.4], "XYStage": [ 43801.100000000006, 31637.9 ] }, "GridColumnIndex": 0 }, { "Label": "Pos2", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.5], "XYStage": [ 38561.3, 32040.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos3", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.185], "Focus": [17741.3], "XYStage": [ 37698.4, 32041.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos4", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.16], "Focus": [17741.5], "XYStage": [ 32375.4, 31758.9 ] }, "GridColumnIndex": 0 }, { "Label": "Pos5", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.17000000000002], "Focus": [17741.3], "XYStage": [ 31770.7, 31758.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos6", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.4], "XYStage": [ 26536.800000000003, 31068.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos7", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.205], "Focus": [17741.4], "XYStage": [ 25420.2, 31068.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos8", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.85], "Focus": [17741.3], "XYStage": [ 21059, 31508 ] }, "GridColumnIndex": 0 }, { "Label": "Pos9", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.82], "Focus": [17741.3], "XYStage": [ 19731.9, 31506.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos10", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.79], "Focus": [17741.4], "XYStage": [ 14528.5, 32007.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos11", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.83], "Focus": [17741.2], "XYStage": [ 13830.300000000001, 32009.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos12", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.79], "Focus": [17741.3], "XYStage": [ 8775.300000000001, 32017.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos13", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.815], "Focus": [17741.3], "XYStage": [ 8109.200000000001, 32008.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos14", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.805], "Focus": [17741.3], "XYStage": [ 3483.3, 31908.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos15", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.82], "Focus": [17741.2], "XYStage": [ 2686.8, 31903.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos16", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [211.48000000000002], "Focus": [17741.1], "XYStage": [ -1978.9, 31973.600000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos17", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [211.51500000000001], "Focus": [17741.1], "XYStage": [ -2957.8, 31982.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos18", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.195], "Focus": [17741.5], "XYStage": [ 47747.4, -576.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos19", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.23000000000002], "Focus": [17741.6], "XYStage": [ 47053.9, -576.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos20", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.205], "Focus": [17741.4], "XYStage": [ 41871.200000000004, -479.8 ] }, "GridColumnIndex": 0 }, { "Label": "Pos21", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.4], "XYStage": [ 40882.600000000006, -392 ] }, "GridColumnIndex": 0 }, { "Label": "Pos22", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.24], "Focus": [17741.4], "XYStage": [ 35905.6, -135.3 ] }, "GridColumnIndex": 0 }, { "Label": "Pos23", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.195], "Focus": [17741.5], "XYStage": [ 34990.9, -132.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos24", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.46], "Focus": [17741.4], "XYStage": [ 29678.100000000002, -958.1 ] }, "GridColumnIndex": 0 }, { "Label": "Pos25", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.44], "Focus": [17741.5], "XYStage": [ 28983.600000000002, -939.4000000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos26", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.475], "Focus": [17741.5], "XYStage": [ 23019.7, -122 ] }, "GridColumnIndex": 0 }, { "Label": "Pos27", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.44], "Focus": [17741.7], "XYStage": [ 22428, -116.80000000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos28", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.46], "Focus": [17741.7], "XYStage": [ 16364.900000000001, -181.10000000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos29", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.48000000000002], "Focus": [17741.5], "XYStage": [ 15712.1, -180 ] }, "GridColumnIndex": 0 }, { "Label": "Pos30", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.45000000000002], "Focus": [17741.7], "XYStage": [ 10346.1, 77.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos31", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.49], "Focus": [17741.4], "XYStage": [ 9755.5, 85.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos32", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.475], "Focus": [17741.5], "XYStage": [ 4775.5, 293.90000000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos33", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.445], "Focus": [17741.4], "XYStage": [ 3954.9, 293.90000000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos34", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.455], "Focus": [17741.4], "XYStage": [ -1324.9, 545.6 ] }, "GridColumnIndex": 0 }, { "Label": "Pos35", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.445], "Focus": [17741.3], "XYStage": [ -2097, 576.6 ] }, "GridColumnIndex": 0 } ], "z-step_um": 10, "GridRow": 0, "BitDepth": 16, "Depth": 2, "Channels": 6, "Source": "Micro-Manager", "ChColors": [ -16711681, -256, -65281, -1, -1, -16776961 ], "MetadataVersion": 10, "KeepShutterOpenChannels": true, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536, 65536 ], "CustomIntervals_ms": [], "Height": 1200, "MicroManagerVersion": "1.4.23 20180219", "GridColumn": 0, "UUID": "67598d21-5db5-4842-ab60-f506f7b42c7c", "PixelType": "GRAY16", "Width": 1200, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\Yan\\20191218-YC113-S7S8-miseq", "Frames": 1, "Comment": "", "UserName": "Nikon", "PixelSize_um": 1.1, "ROI": [ 0, 0, 1200, 1200 ], "Date": "2019-12-18", "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC", "P-DAPI-high" ], "KeepShutterOpenSlices": true, "IJType": 1, "ComputerName": "ZadorConfocal2", "Positions": 36, "PositionIndex": 29, "CameraTimeout": 20000, "TimeFirst": false }, "640 AO-Name": "AnalogIO", "Vincent-D3-Command": "Close" }, "FrameKey-0-0-3": { "COM4-StopBits": "1", "528 AO-MaxVolts": "5.0000", "XLIGHT Excitation Wheel-State": "1", "470 Shutter-Name": "AnalogIO", "Core-Shutter": "520", "555 AO-Demo": "No", "XLIGHT Dichroic-State": "2", "XYStage-StepSize-Y": "0.1000", "ZPositionUm": 198.48000000000002, "XYStage-StepSize-X": "0.1000", "PRIME-FirmwareVersion": "20.28", "PRIME-ClearCycles": "2", "520-Name": "DA Shutter", "405 Shutter-Name": "AnalogIO", "PIEZO-StepSize": "0.0050", "PRIME-ClearMode": "Pre-Exposure", "470 AO-MinVolts": "0.0000", "405 AO-SupportsTriggering": "1", "CameraChannelIndex": 0, "XYStage-StartSpeed": "500.0000", "COM9-StopBits": "2", "405 AO-Description": "NI DAC", "COM4-DelayBetweenCharsMs": "0.0000", "445 AO-Block voltage": "false", "520 AO-TriggerInputLine": "/Dev1/PFI0", "555 AO-Description": "NI DAC", "520 AO-TriggerSampleRate": "100000", "COM4-Verbose": "1", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM4-Name": "COM4", "405 Shutter-Volts": "0.0000", "Vincent-D3-Port": "COM1", "PRIME-PP 4 MIN ADU AFFECTED": "200", "470 Shutter-MinVolts": "0.0000", "PRIME-PP 3 ENABLED": "Yes", "470 AO-Description": "NI DAC", "PRIME-Description": "PVCAM API device adapter", "520 Shutter-MaxVolts": "5.0000", "520 Shutter-Volts": "5.0000", "528 AO-Sequenceable": "1", "PRIME-Binning": "1x1", "555 Shutter-MaxVolts": "5.0000", "528 Shutter-TriggerSequenceLength": "1000", "405 Shutter-Block voltage": "false", "XLIGHT Spin motor-State": "1", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "Vincent-D3-Time to open (ms)": "35.0000", "Vincent-D3-Time to close (ms)": "35.0000", "445 AO-TriggerSampleRate": "100000", "640 Shutter-IOChannel": "Dev1/ao14", "555 AO-Sequenceable": "1", "520 AO-Name": "AnalogIO", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "470 AO-Block voltage": "false", "COM1-Verbose": "1", "COM9-AnswerTimeout": "10000.0000", "528 Shutter-Name": "AnalogIO", "PRIME-Trigger Timeout (secs)": "10", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "NextFrame": 0, "470 AO-TriggerSequenceLength": "1000", "PRIME-TransposeXY": "0", "445 AO-TriggerSequenceLength": "1000", "COM1-Name": "COM1", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "COM9-DataBits": "8", "COM1-AnswerTimeout": "500.0000", "Camera": "", "TE2000-Version": "V2.50-V2.50-V2.10", "528 Shutter-Volts": "0.0000", "XLIGHT Hub-Name": "XLIGHT Hub", "PIEZO-Description": "Ludl stage driver adapter", "PositionName": "Pos29", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSampleRate": "100000", "PRIME-FanSpeedSetpoint": "High", "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "405 AO-TriggerSampleRate": "100000", "470 Shutter-Block voltage": "false", "470-State": "0", "COM3-Parity": "None", "405 AO-Volts": "0.0000", "PRIME-TransposeMirrorY": "0", "PRIME-ChipName": "GS144BSI", "470 AO-Volts": "0.0000", "PRIME-TransposeMirrorX": "0", "445 Shutter-SupportsTriggering": "0", "470 Shutter-SupportsTriggering": "0", "XLIGHT Hub-Description": "XLIGHT hub", "PRIME-PreampOffLimit": "10000", "470 AO-Sequenceable": "1", "528 Shutter-MaxVolts": "5.0000", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "Core-XYStage": "XYStage", "470 AO-TriggerSampleRate": "100000", "PRIME-Timing-PreTriggerDelayNs": "24672000.0000", "528-DA Device": "528 Shutter", "Focus-Description": "Nikon TE2000 focus adapter", "FilterBlock-State": "5", "520 AO-Demo": "No", "COM3-DelayBetweenCharsMs": "0.0000", "470 Shutter-Volts": "0.0000", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "PRIME-PP 2 THRESHOLD": "125", "PRIME-PP 4 THRESHOLD": "75", "XYStage-Acceleration": "4.0000", "470 Shutter-Demo": "No", "PRIME-Name": "PMPCIECam00", "640-DA Device": "640 Shutter", "405 AO-Block voltage": "false", "ElapsedTime-ms": 1192053, "PRIME-Offset": "100", "445 AO-IOChannel": "Dev1/ao1", "640 AO-MaxVolts": "5.0000", "PositionIndex": 29, "PRIME-PP 1 ENABLED": "Yes", "COM1-BaudRate": "9600", "640-State": "0", "COM3-Verbose": "1", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "LudlController-TransmissionDelay": "0", "PRIME-FullWellCapacity": "62000", "PRIME-SMARTStreamingEnabled": "No", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "640 Shutter-Description": "NI DAC", "528-Description": "DA device that is used as a shutter", "COM4-Description": "Serial port driver (boost:asio)", "405 Shutter-Description": "NI DAC", "TE2000-Name": "TE2000", "528 Shutter-Description": "NI DAC", "COM9-Description": "Serial port driver (boost:asio)", "LudlController-Name": "LudlController", "445 Shutter-TriggerSequenceLength": "1000", "PRIME-SerialNumber": "A18B203008", "445 AO-Volts": "0.0000", "PRIME-X-dimension": "1200", "PRIME-ShutterCloseDelay": "0", "XLIGHT Disk slider-State": "2", "Position": "Default", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 AO-IOChannel": "Dev1/ao5", "520 AO-IOChannel": "Dev1/ao4", "528 AO-TriggerSequenceLength": "1000", "405 Shutter-MaxVolts": "5.0000", "470-DA Device": "470 Shutter", "PRIME-FTCapable": "No", "Core-ChannelGroup": "Channels", "555 AO-SupportsTriggering": "0", "PRIME-Y-dimension": "1200", "Frame": 0, "555 Shutter-Sequenceable": "1", "PRIME-Timing-ExposureTimeNs": "499998000.0000", "Core-ImageProcessor": "", "528-State": "0", "520 AO-Block voltage": "false", "XLIGHT Excitation Wheel-Label": "CFP/YFP", "640 Shutter-Demo": "No", "COM3-StopBits": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "520 Shutter-IOChannel": "Dev1/ao11", "Width": 1200, "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-SupportsTriggering": "0", "640 AO-TriggerSampleRate": "100000", "520-Description": "DA device that is used as a shutter", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "405 Shutter-Sequenceable": "1", "405 Shutter-SupportsTriggering": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-TriggerSampleRate": "100000", "640 Shutter-MaxVolts": "5.0000", "555 AO-MaxVolts": "5.0000", "520 AO-TriggerSequenceLength": "1000", "COM3-BaudRate": "9600", "COM9-BaudRate": "9600", "Vincent-D3-Channel #": "Ch. #1", "PRIME-PMode": "Normal", "OpticalPath-Label": "Left(100)", "BitDepth": 16, "555 AO-TriggerSequenceLength": "1000", "445 Shutter-Description": "NI DAC", "405 AO-Sequenceable": "1", "PRIME-AcquisitionMethod": "Callbacks", "555 AO-Name": "AnalogIO", "PRIME-ShutterMode": "Pre-Exposure", "COM3-Handshaking": "Off", "445 AO-SupportsTriggering": "0", "ROI": "0-0-1200-1200", "COM9-Verbose": "1", "PRIME-Color": "OFF", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "PRIME-ShutterOpenDelay": "0", "COM1-Parity": "None", "Slice": 3, "SlicePosition": -20, "COM9-DelayBetweenCharsMs": "0.0000", "555-Name": "DA Shutter", "PIEZO-LudlSingleAxisName": "B", "XLIGHT Emission Wheel-Label": "565/24(G)", "640 Shutter-Name": "AnalogIO", "640 AO-Sequenceable": "1", "520 Shutter-Demo": "No", "528 Shutter-Demo": "No", "470-Name": "DA Shutter", "520-DA Device": "520 Shutter", "XYStage-Description": "Ludl XY stage driver adapter", "445 Shutter-MinVolts": "0.0000", "528 AO-TriggerSampleRate": "100000", "405 AO-MinVolts": "0.0000", "405 Shutter-TriggerSequenceLength": "1000", "Height": 1200, "555 AO-Block voltage": "false", "640 Shutter-Volts": "0.0000", "PixelType": "GRAY16", "445 Shutter-Block voltage": "false", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "520 Shutter-TriggerSequenceLength": "1000", "COM9-Name": "COM9", "Core-SLM": "", "555 AO-Volts": "0.0000", "555 Shutter-Block voltage": "false", "XYStage-Speed": "1800.0000", "520 AO-MaxVolts": "5.0000", "528 Shutter-SupportsTriggering": "0", "COM1-Description": "Serial port driver (boost:asio)", "PRIME-MetadataEnabled": "No", "520 Shutter-MinVolts": "0.0000", "470 Shutter-Sequenceable": "1", "PRIME-PreampDelay": "0", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-MinVolts": "0.0000", "ChannelIndex": 0, "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Core-AutoShutter": "1", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "Exposure-ms": 320, "PRIME-PP 2 ENABLED": "Yes", "640 Shutter-TriggerSampleRate": "100000", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "470 Shutter-IOChannel": "Dev1/ao10", "405 AO-Demo": "No", "PixelSizeUm": 1.1, "470 AO-Demo": "No", "470 Shutter-MaxVolts": "5.0000", "Vincent-D3-Name": "Vincent-D3", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FileName": "img_000000000_Il-G_003.tif", "LudlController-Reset": "Operate", "PRIME-Exposure": "320.00", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "445-Name": "DA Shutter", "555 Shutter-Name": "AnalogIO", "520 Shutter-TriggerSampleRate": "100000", "445 Shutter-IOChannel": "Dev1/ao9", "PRIME-TransposeCorrection": "0", "XYStage-StepSize": "0.1000", "445-Description": "DA device that is used as a shutter", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 AO-IOChannel": "Dev1/ao7", "520 Shutter-Name": "AnalogIO", "COM3-DataBits": "8", "640 AO-Volts": "0.0000", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "555 Shutter-IOChannel": "Dev1/ao13", "WaitInterval": null, "520 Shutter-Sequenceable": "1", "YPositionUm": -180, "445-State": "0", "445-DA Device": "445 Shutter", "PRIME-CircularBufferAutoSize": "ON", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "555 AO-TriggerSampleRate": "100000", "445 Shutter-Volts": "0.0000", "XLIGHT Hub-Port": "COM3", "Core-AutoFocus": "", "COM4-Handshaking": "Off", "528 Shutter-MinVolts": "0.0000", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "445 Shutter-Name": "AnalogIO", "528 Shutter-Sequenceable": "1", "XYStage-TransposeMirrorX": "0", "XYStage-TransposeMirrorY": "0", "PRIME-ReadoutRate": "100MHz 16bit", "XYStage-Name": "Stage", "640 AO-SupportsTriggering": "0", "640-Name": "DA Shutter", "640 Shutter-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "640 AO-Block voltage": "false", "445 Shutter-Sequenceable": "1", "470 AO-MaxVolts": "5.0000", "COM4-BaudRate": "9600", "470-Description": "DA device that is used as a shutter", "520 Shutter-Description": "NI DAC", "PRIME-CCDTemperature": "-14.8500", "Time": "2019-12-18 23:10:52 -0500", "640 AO-TriggerSequenceLength": "1000", "470 AO-Name": "AnalogIO", "XLIGHT Disk slider-Label": "Disk Pos 40um", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "Binning": "1x1", "TE2000-ModelType": "1", "COM3-AnswerTimeout": "500.0000", "555 Shutter-SupportsTriggering": "0", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "TE2000-Port": "COM4", "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Description": "NI DAC", "COM4-AnswerTimeout": "500.0000", "COM1-DelayBetweenCharsMs": "0.0000", "FilterBlock-Label": "Confocal", "445 AO-Name": "AnalogIO", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-Timing-ReadoutTimeNs": "24672000.0000", "528 AO-Demo": "No", "520 AO-Sequenceable": "1", "555-Description": "DA device that is used as a shutter", "405-DA Device": "405 Shutter", "405 AO-TriggerSequenceLength": "1000", "640 AO-MinVolts": "0.0000", "640 Shutter-Block voltage": "false", "FrameIndex": 0, "PRIME-Port": "CMOS", "555 AO-MinVolts": "0.0000", "COM3-Description": "Serial port driver (boost:asio)", "528 AO-Description": "NI DAC", "555 AO-IOChannel": "Dev1/ao6", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "555 AO-TriggerInputLine": "/Dev1/PFI0", "PRIME-ActualInterval-ms": "390.5200", "LudlController-Port": "COM9", "445 Shutter-Demo": "No", "PRIME-Trigger-Expose Out-Mux": "1", "SliceIndex": 3, "PRIME-CCDTemperatureSetPoint": "-15.0000", "405 Shutter-MinVolts": "0.0000", "PRIME-ExposeOutMode": "First Row", "AxisPositions": null, "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Volts": "0.0000", "528-Name": "DA Shutter", "555-State": "0", "XLIGHT Dichroic-Label": "CFP/YFP", "445 AO-Sequenceable": "1", "405-Name": "DA Shutter", "405 AO-IOChannel": "Dev1/ao0", "Core-Camera": "PRIME", "Focus-Name": "Focus", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "405 Shutter-IOChannel": "Dev1/ao8", "640 AO-Demo": "No", "640 Shutter-SupportsTriggering": "0", "445 AO-Demo": "No", "555-DA Device": "555 Shutter", "470 Shutter-Description": "NI DAC", "528 AO-Volts": "0.0000", "COM1-DataBits": "8", "Vincent-D3-Address": "x", "520 AO-SupportsTriggering": "0", "405-Description": "DA device that is used as a shutter", "640 Shutter-MinVolts": "0.0000", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "405 AO-Name": "AnalogIO", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "640 AO-TriggerInputLine": "/Dev1/PFI0", "405 AO-TriggerInputLine": "/Dev1/PFI0", "640-Description": "DA device that is used as a shutter", "445 Shutter-TriggerSampleRate": "100000", "470 Shutter-TriggerSequenceLength": "1000", "COM1-StopBits": "1", "520 Shutter-SupportsTriggering": "0", "405-State": "0", "Core-Initialize": "1", "XLIGHT Emission Wheel-State": "3", "520 AO-Description": "NI DAC", "528 AO-TriggerInputLine": "/Dev1/PFI0", "445 Shutter-MaxVolts": "5.0000", "COM9-Handshaking": "Off", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "XLIGHT Spin motor-Label": "On (spinning)", "PRIME-PP 5 ENABLED": "No", "UUID": "4d804080-72e8-4817-9b06-c02ceb2edcb7", "PRIME-TriggerMode": "Internal Trigger", "COM1-Handshaking": "Off", "PRIME-Timing-PostTriggerDelayNs": "49344000.0000", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "Focus-Position": "17741.5000", "528 Shutter-Block voltage": "false", "Channel": "Il-G", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "555 Shutter-Demo": "No", "405 Shutter-Demo": "No", "528 AO-Name": "AnalogIO", "520-State": "1", "Core-Galvo": "", "405 Shutter-TriggerSampleRate": "100000", "Focus-FocusStepSize_(nm)": "100", "PRIME-PixelType": "16bit", "COM4-DataBits": "8", "470 AO-IOChannel": "Dev1/ao2", "520 AO-MinVolts": "0.0000", "COM9-Parity": "None", "PRIME-CircularBufferEnabled": "ON", "PRIME-PP 6 Reset": "No", "640 AO-Description": "NI DAC", "Source": "PRIME", "445 AO-MinVolts": "0.0000", "PIEZO-Name": "Stage", "445 AO-Description": "NI DAC", "PRIME-CircularBufferFrameCount": "46", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "520 AO-Volts": "4.5000", "528 Shutter-TriggerSampleRate": "100000", "XPositionUm": 15712.1, "OpticalPath-State": "4", "PRIME-PP 3 THRESHOLD": "80", "PRIME-PVCAM Version": "3.7.1", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Gain": "1-HDR", "640 Shutter-TriggerSequenceLength": "1000", "528 AO-SupportsTriggering": "0", "445 AO-TriggerInputLine": "/Dev1/PFI0", "OpticalPath-Name": "OpticalPath", "PRIME-PP 4 ENABLED": "Yes", "PRIME-PVCAM Adapter Version": "1.3.29", "COM3-Name": "COM3", "405 AO-MaxVolts": "5.0000", "520 Shutter-Block voltage": "false", "PRIME-Timing-ClearingTimeNs": "49344000.0000", "Core-Focus": "PIEZO", "528 AO-Block voltage": "false", "TE2000-Description": "Nikon TE2000 hub", "528 AO-MinVolts": "0.0000", "COM4-Parity": "None", "Summary": { "Slices": 11, "ChContrastMin": [ 0, 0, 0, 0, 0, 0 ], "PixelAspect": 1, "SlicesFirst": true, "Interval_ms": 0, "Time": "2019-12-18 23:10:50 -0500", "Prefix": "seq_5", "InitialPositionList": [ { "Label": "Pos0", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.21], "Focus": [17741.5], "XYStage": [ 44542.200000000004, 31636.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos1", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.22], "Focus": [17741.4], "XYStage": [ 43801.100000000006, 31637.9 ] }, "GridColumnIndex": 0 }, { "Label": "Pos2", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.5], "XYStage": [ 38561.3, 32040.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos3", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.185], "Focus": [17741.3], "XYStage": [ 37698.4, 32041.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos4", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.16], "Focus": [17741.5], "XYStage": [ 32375.4, 31758.9 ] }, "GridColumnIndex": 0 }, { "Label": "Pos5", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.17000000000002], "Focus": [17741.3], "XYStage": [ 31770.7, 31758.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos6", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.4], "XYStage": [ 26536.800000000003, 31068.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos7", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.205], "Focus": [17741.4], "XYStage": [ 25420.2, 31068.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos8", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.85], "Focus": [17741.3], "XYStage": [ 21059, 31508 ] }, "GridColumnIndex": 0 }, { "Label": "Pos9", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.82], "Focus": [17741.3], "XYStage": [ 19731.9, 31506.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos10", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.79], "Focus": [17741.4], "XYStage": [ 14528.5, 32007.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos11", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.83], "Focus": [17741.2], "XYStage": [ 13830.300000000001, 32009.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos12", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.79], "Focus": [17741.3], "XYStage": [ 8775.300000000001, 32017.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos13", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.815], "Focus": [17741.3], "XYStage": [ 8109.200000000001, 32008.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos14", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.805], "Focus": [17741.3], "XYStage": [ 3483.3, 31908.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos15", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.82], "Focus": [17741.2], "XYStage": [ 2686.8, 31903.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos16", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [211.48000000000002], "Focus": [17741.1], "XYStage": [ -1978.9, 31973.600000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos17", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [211.51500000000001], "Focus": [17741.1], "XYStage": [ -2957.8, 31982.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos18", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.195], "Focus": [17741.5], "XYStage": [ 47747.4, -576.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos19", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.23000000000002], "Focus": [17741.6], "XYStage": [ 47053.9, -576.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos20", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.205], "Focus": [17741.4], "XYStage": [ 41871.200000000004, -479.8 ] }, "GridColumnIndex": 0 }, { "Label": "Pos21", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.4], "XYStage": [ 40882.600000000006, -392 ] }, "GridColumnIndex": 0 }, { "Label": "Pos22", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.24], "Focus": [17741.4], "XYStage": [ 35905.6, -135.3 ] }, "GridColumnIndex": 0 }, { "Label": "Pos23", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.195], "Focus": [17741.5], "XYStage": [ 34990.9, -132.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos24", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.46], "Focus": [17741.4], "XYStage": [ 29678.100000000002, -958.1 ] }, "GridColumnIndex": 0 }, { "Label": "Pos25", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.44], "Focus": [17741.5], "XYStage": [ 28983.600000000002, -939.4000000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos26", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.475], "Focus": [17741.5], "XYStage": [ 23019.7, -122 ] }, "GridColumnIndex": 0 }, { "Label": "Pos27", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.44], "Focus": [17741.7], "XYStage": [ 22428, -116.80000000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos28", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.46], "Focus": [17741.7], "XYStage": [ 16364.900000000001, -181.10000000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos29", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.48000000000002], "Focus": [17741.5], "XYStage": [ 15712.1, -180 ] }, "GridColumnIndex": 0 }, { "Label": "Pos30", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.45000000000002], "Focus": [17741.7], "XYStage": [ 10346.1, 77.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos31", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.49], "Focus": [17741.4], "XYStage": [ 9755.5, 85.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos32", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.475], "Focus": [17741.5], "XYStage": [ 4775.5, 293.90000000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos33", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.445], "Focus": [17741.4], "XYStage": [ 3954.9, 293.90000000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos34", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.455], "Focus": [17741.4], "XYStage": [ -1324.9, 545.6 ] }, "GridColumnIndex": 0 }, { "Label": "Pos35", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.445], "Focus": [17741.3], "XYStage": [ -2097, 576.6 ] }, "GridColumnIndex": 0 } ], "z-step_um": 10, "GridRow": 0, "BitDepth": 16, "Depth": 2, "Channels": 6, "Source": "Micro-Manager", "ChColors": [ -16711681, -256, -65281, -1, -1, -16776961 ], "MetadataVersion": 10, "KeepShutterOpenChannels": true, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536, 65536 ], "CustomIntervals_ms": [], "Height": 1200, "MicroManagerVersion": "1.4.23 20180219", "GridColumn": 0, "UUID": "67598d21-5db5-4842-ab60-f506f7b42c7c", "PixelType": "GRAY16", "Width": 1200, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\Yan\\20191218-YC113-S7S8-miseq", "Frames": 1, "Comment": "", "UserName": "Nikon", "PixelSize_um": 1.1, "ROI": [ 0, 0, 1200, 1200 ], "Date": "2019-12-18", "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC", "P-DAPI-high" ], "KeepShutterOpenSlices": true, "IJType": 1, "ComputerName": "ZadorConfocal2", "Positions": 36, "PositionIndex": 29, "CameraTimeout": 20000, "TimeFirst": false }, "640 AO-Name": "AnalogIO", "Vincent-D3-Command": "Close" }, "FrameKey-0-0-4": { "COM4-StopBits": "1", "528 AO-MaxVolts": "5.0000", "XLIGHT Excitation Wheel-State": "1", "470 Shutter-Name": "AnalogIO", "Core-Shutter": "520", "555 AO-Demo": "No", "XLIGHT Dichroic-State": "2", "XYStage-StepSize-Y": "0.1000", "ZPositionUm": 208.48000000000002, "XYStage-StepSize-X": "0.1000", "PRIME-FirmwareVersion": "20.28", "PRIME-ClearCycles": "2", "520-Name": "DA Shutter", "405 Shutter-Name": "AnalogIO", "PIEZO-StepSize": "0.0050", "PRIME-ClearMode": "Pre-Exposure", "470 AO-MinVolts": "0.0000", "405 AO-SupportsTriggering": "1", "CameraChannelIndex": 0, "XYStage-StartSpeed": "500.0000", "COM9-StopBits": "2", "405 AO-Description": "NI DAC", "COM4-DelayBetweenCharsMs": "0.0000", "445 AO-Block voltage": "false", "520 AO-TriggerInputLine": "/Dev1/PFI0", "555 AO-Description": "NI DAC", "520 AO-TriggerSampleRate": "100000", "COM4-Verbose": "1", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM4-Name": "COM4", "405 Shutter-Volts": "0.0000", "Vincent-D3-Port": "COM1", "PRIME-PP 4 MIN ADU AFFECTED": "200", "470 Shutter-MinVolts": "0.0000", "PRIME-PP 3 ENABLED": "Yes", "470 AO-Description": "NI DAC", "PRIME-Description": "PVCAM API device adapter", "520 Shutter-MaxVolts": "5.0000", "520 Shutter-Volts": "5.0000", "528 AO-Sequenceable": "1", "PRIME-Binning": "1x1", "555 Shutter-MaxVolts": "5.0000", "528 Shutter-TriggerSequenceLength": "1000", "405 Shutter-Block voltage": "false", "XLIGHT Spin motor-State": "1", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "Vincent-D3-Time to open (ms)": "35.0000", "Vincent-D3-Time to close (ms)": "35.0000", "445 AO-TriggerSampleRate": "100000", "640 Shutter-IOChannel": "Dev1/ao14", "555 AO-Sequenceable": "1", "520 AO-Name": "AnalogIO", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "470 AO-Block voltage": "false", "COM1-Verbose": "1", "COM9-AnswerTimeout": "10000.0000", "528 Shutter-Name": "AnalogIO", "PRIME-Trigger Timeout (secs)": "10", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "NextFrame": 0, "470 AO-TriggerSequenceLength": "1000", "PRIME-TransposeXY": "0", "445 AO-TriggerSequenceLength": "1000", "COM1-Name": "COM1", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "COM9-DataBits": "8", "COM1-AnswerTimeout": "500.0000", "Camera": "", "TE2000-Version": "V2.50-V2.50-V2.10", "528 Shutter-Volts": "0.0000", "XLIGHT Hub-Name": "XLIGHT Hub", "PIEZO-Description": "Ludl stage driver adapter", "PositionName": "Pos29", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSampleRate": "100000", "PRIME-FanSpeedSetpoint": "High", "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "405 AO-TriggerSampleRate": "100000", "470 Shutter-Block voltage": "false", "470-State": "0", "COM3-Parity": "None", "405 AO-Volts": "0.0000", "PRIME-TransposeMirrorY": "0", "PRIME-ChipName": "GS144BSI", "470 AO-Volts": "0.0000", "PRIME-TransposeMirrorX": "0", "445 Shutter-SupportsTriggering": "0", "470 Shutter-SupportsTriggering": "0", "XLIGHT Hub-Description": "XLIGHT hub", "PRIME-PreampOffLimit": "10000", "470 AO-Sequenceable": "1", "528 Shutter-MaxVolts": "5.0000", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "Core-XYStage": "XYStage", "470 AO-TriggerSampleRate": "100000", "PRIME-Timing-PreTriggerDelayNs": "24672000.0000", "528-DA Device": "528 Shutter", "Focus-Description": "Nikon TE2000 focus adapter", "FilterBlock-State": "5", "520 AO-Demo": "No", "COM3-DelayBetweenCharsMs": "0.0000", "470 Shutter-Volts": "0.0000", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "PRIME-PP 2 THRESHOLD": "125", "PRIME-PP 4 THRESHOLD": "75", "XYStage-Acceleration": "4.0000", "470 Shutter-Demo": "No", "PRIME-Name": "PMPCIECam00", "640-DA Device": "640 Shutter", "405 AO-Block voltage": "false", "ElapsedTime-ms": 1192616, "PRIME-Offset": "100", "445 AO-IOChannel": "Dev1/ao1", "640 AO-MaxVolts": "5.0000", "PositionIndex": 29, "PRIME-PP 1 ENABLED": "Yes", "COM1-BaudRate": "9600", "640-State": "0", "COM3-Verbose": "1", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "LudlController-TransmissionDelay": "0", "PRIME-FullWellCapacity": "62000", "PRIME-SMARTStreamingEnabled": "No", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "640 Shutter-Description": "NI DAC", "528-Description": "DA device that is used as a shutter", "COM4-Description": "Serial port driver (boost:asio)", "405 Shutter-Description": "NI DAC", "TE2000-Name": "TE2000", "528 Shutter-Description": "NI DAC", "COM9-Description": "Serial port driver (boost:asio)", "LudlController-Name": "LudlController", "445 Shutter-TriggerSequenceLength": "1000", "PRIME-SerialNumber": "A18B203008", "445 AO-Volts": "0.0000", "PRIME-X-dimension": "1200", "PRIME-ShutterCloseDelay": "0", "XLIGHT Disk slider-State": "2", "Position": "Default", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 AO-IOChannel": "Dev1/ao5", "520 AO-IOChannel": "Dev1/ao4", "528 AO-TriggerSequenceLength": "1000", "405 Shutter-MaxVolts": "5.0000", "470-DA Device": "470 Shutter", "PRIME-FTCapable": "No", "Core-ChannelGroup": "Channels", "555 AO-SupportsTriggering": "0", "PRIME-Y-dimension": "1200", "Frame": 0, "555 Shutter-Sequenceable": "1", "PRIME-Timing-ExposureTimeNs": "499998000.0000", "Core-ImageProcessor": "", "528-State": "0", "520 AO-Block voltage": "false", "XLIGHT Excitation Wheel-Label": "CFP/YFP", "640 Shutter-Demo": "No", "COM3-StopBits": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "520 Shutter-IOChannel": "Dev1/ao11", "Width": 1200, "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-SupportsTriggering": "0", "640 AO-TriggerSampleRate": "100000", "520-Description": "DA device that is used as a shutter", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "405 Shutter-Sequenceable": "1", "405 Shutter-SupportsTriggering": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-TriggerSampleRate": "100000", "640 Shutter-MaxVolts": "5.0000", "555 AO-MaxVolts": "5.0000", "520 AO-TriggerSequenceLength": "1000", "COM3-BaudRate": "9600", "COM9-BaudRate": "9600", "Vincent-D3-Channel #": "Ch. #1", "PRIME-PMode": "Normal", "OpticalPath-Label": "Left(100)", "BitDepth": 16, "555 AO-TriggerSequenceLength": "1000", "445 Shutter-Description": "NI DAC", "405 AO-Sequenceable": "1", "PRIME-AcquisitionMethod": "Callbacks", "555 AO-Name": "AnalogIO", "PRIME-ShutterMode": "Pre-Exposure", "COM3-Handshaking": "Off", "445 AO-SupportsTriggering": "0", "ROI": "0-0-1200-1200", "COM9-Verbose": "1", "PRIME-Color": "OFF", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "PRIME-ShutterOpenDelay": "0", "COM1-Parity": "None", "Slice": 4, "SlicePosition": -10, "COM9-DelayBetweenCharsMs": "0.0000", "555-Name": "DA Shutter", "PIEZO-LudlSingleAxisName": "B", "XLIGHT Emission Wheel-Label": "565/24(G)", "640 Shutter-Name": "AnalogIO", "640 AO-Sequenceable": "1", "520 Shutter-Demo": "No", "528 Shutter-Demo": "No", "470-Name": "DA Shutter", "520-DA Device": "520 Shutter", "XYStage-Description": "Ludl XY stage driver adapter", "445 Shutter-MinVolts": "0.0000", "528 AO-TriggerSampleRate": "100000", "405 AO-MinVolts": "0.0000", "405 Shutter-TriggerSequenceLength": "1000", "Height": 1200, "555 AO-Block voltage": "false", "640 Shutter-Volts": "0.0000", "PixelType": "GRAY16", "445 Shutter-Block voltage": "false", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "520 Shutter-TriggerSequenceLength": "1000", "COM9-Name": "COM9", "Core-SLM": "", "555 AO-Volts": "0.0000", "555 Shutter-Block voltage": "false", "XYStage-Speed": "1800.0000", "520 AO-MaxVolts": "5.0000", "528 Shutter-SupportsTriggering": "0", "COM1-Description": "Serial port driver (boost:asio)", "PRIME-MetadataEnabled": "No", "520 Shutter-MinVolts": "0.0000", "470 Shutter-Sequenceable": "1", "PRIME-PreampDelay": "0", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-MinVolts": "0.0000", "ChannelIndex": 0, "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Core-AutoShutter": "1", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "Exposure-ms": 320, "PRIME-PP 2 ENABLED": "Yes", "640 Shutter-TriggerSampleRate": "100000", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "470 Shutter-IOChannel": "Dev1/ao10", "405 AO-Demo": "No", "PixelSizeUm": 1.1, "470 AO-Demo": "No", "470 Shutter-MaxVolts": "5.0000", "Vincent-D3-Name": "Vincent-D3", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FileName": "img_000000000_Il-G_004.tif", "LudlController-Reset": "Operate", "PRIME-Exposure": "320.00", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "445-Name": "DA Shutter", "555 Shutter-Name": "AnalogIO", "520 Shutter-TriggerSampleRate": "100000", "445 Shutter-IOChannel": "Dev1/ao9", "PRIME-TransposeCorrection": "0", "XYStage-StepSize": "0.1000", "445-Description": "DA device that is used as a shutter", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 AO-IOChannel": "Dev1/ao7", "520 Shutter-Name": "AnalogIO", "COM3-DataBits": "8", "640 AO-Volts": "0.0000", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "555 Shutter-IOChannel": "Dev1/ao13", "WaitInterval": null, "520 Shutter-Sequenceable": "1", "YPositionUm": -180, "445-State": "0", "445-DA Device": "445 Shutter", "PRIME-CircularBufferAutoSize": "ON", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "555 AO-TriggerSampleRate": "100000", "445 Shutter-Volts": "0.0000", "XLIGHT Hub-Port": "COM3", "Core-AutoFocus": "", "COM4-Handshaking": "Off", "528 Shutter-MinVolts": "0.0000", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "445 Shutter-Name": "AnalogIO", "528 Shutter-Sequenceable": "1", "XYStage-TransposeMirrorX": "0", "XYStage-TransposeMirrorY": "0", "PRIME-ReadoutRate": "100MHz 16bit", "XYStage-Name": "Stage", "640 AO-SupportsTriggering": "0", "640-Name": "DA Shutter", "640 Shutter-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "640 AO-Block voltage": "false", "445 Shutter-Sequenceable": "1", "470 AO-MaxVolts": "5.0000", "COM4-BaudRate": "9600", "470-Description": "DA device that is used as a shutter", "520 Shutter-Description": "NI DAC", "PRIME-CCDTemperature": "-14.8500", "Time": "2019-12-18 23:10:52 -0500", "640 AO-TriggerSequenceLength": "1000", "470 AO-Name": "AnalogIO", "XLIGHT Disk slider-Label": "Disk Pos 40um", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "Binning": "1x1", "TE2000-ModelType": "1", "COM3-AnswerTimeout": "500.0000", "555 Shutter-SupportsTriggering": "0", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "TE2000-Port": "COM4", "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Description": "NI DAC", "COM4-AnswerTimeout": "500.0000", "COM1-DelayBetweenCharsMs": "0.0000", "FilterBlock-Label": "Confocal", "445 AO-Name": "AnalogIO", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-Timing-ReadoutTimeNs": "24672000.0000", "528 AO-Demo": "No", "520 AO-Sequenceable": "1", "555-Description": "DA device that is used as a shutter", "405-DA Device": "405 Shutter", "405 AO-TriggerSequenceLength": "1000", "640 AO-MinVolts": "0.0000", "640 Shutter-Block voltage": "false", "FrameIndex": 0, "PRIME-Port": "CMOS", "555 AO-MinVolts": "0.0000", "COM3-Description": "Serial port driver (boost:asio)", "528 AO-Description": "NI DAC", "555 AO-IOChannel": "Dev1/ao6", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "555 AO-TriggerInputLine": "/Dev1/PFI0", "PRIME-ActualInterval-ms": "390.5200", "LudlController-Port": "COM9", "445 Shutter-Demo": "No", "PRIME-Trigger-Expose Out-Mux": "1", "SliceIndex": 4, "PRIME-CCDTemperatureSetPoint": "-15.0000", "405 Shutter-MinVolts": "0.0000", "PRIME-ExposeOutMode": "First Row", "AxisPositions": null, "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Volts": "0.0000", "528-Name": "DA Shutter", "555-State": "0", "XLIGHT Dichroic-Label": "CFP/YFP", "445 AO-Sequenceable": "1", "405-Name": "DA Shutter", "405 AO-IOChannel": "Dev1/ao0", "Core-Camera": "PRIME", "Focus-Name": "Focus", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "405 Shutter-IOChannel": "Dev1/ao8", "640 AO-Demo": "No", "640 Shutter-SupportsTriggering": "0", "445 AO-Demo": "No", "555-DA Device": "555 Shutter", "470 Shutter-Description": "NI DAC", "528 AO-Volts": "0.0000", "COM1-DataBits": "8", "Vincent-D3-Address": "x", "520 AO-SupportsTriggering": "0", "405-Description": "DA device that is used as a shutter", "640 Shutter-MinVolts": "0.0000", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "405 AO-Name": "AnalogIO", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "640 AO-TriggerInputLine": "/Dev1/PFI0", "405 AO-TriggerInputLine": "/Dev1/PFI0", "640-Description": "DA device that is used as a shutter", "445 Shutter-TriggerSampleRate": "100000", "470 Shutter-TriggerSequenceLength": "1000", "COM1-StopBits": "1", "520 Shutter-SupportsTriggering": "0", "405-State": "0", "Core-Initialize": "1", "XLIGHT Emission Wheel-State": "3", "520 AO-Description": "NI DAC", "528 AO-TriggerInputLine": "/Dev1/PFI0", "445 Shutter-MaxVolts": "5.0000", "COM9-Handshaking": "Off", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "XLIGHT Spin motor-Label": "On (spinning)", "PRIME-PP 5 ENABLED": "No", "UUID": "16ba3722-4264-462d-aa43-249bf1cfdbfb", "PRIME-TriggerMode": "Internal Trigger", "COM1-Handshaking": "Off", "PRIME-Timing-PostTriggerDelayNs": "49344000.0000", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "Focus-Position": "17741.5000", "528 Shutter-Block voltage": "false", "Channel": "Il-G", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "555 Shutter-Demo": "No", "405 Shutter-Demo": "No", "528 AO-Name": "AnalogIO", "520-State": "1", "Core-Galvo": "", "405 Shutter-TriggerSampleRate": "100000", "Focus-FocusStepSize_(nm)": "100", "PRIME-PixelType": "16bit", "COM4-DataBits": "8", "470 AO-IOChannel": "Dev1/ao2", "520 AO-MinVolts": "0.0000", "COM9-Parity": "None", "PRIME-CircularBufferEnabled": "ON", "PRIME-PP 6 Reset": "No", "640 AO-Description": "NI DAC", "Source": "PRIME", "445 AO-MinVolts": "0.0000", "PIEZO-Name": "Stage", "445 AO-Description": "NI DAC", "PRIME-CircularBufferFrameCount": "46", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "520 AO-Volts": "4.5000", "528 Shutter-TriggerSampleRate": "100000", "XPositionUm": 15712.1, "OpticalPath-State": "4", "PRIME-PP 3 THRESHOLD": "80", "PRIME-PVCAM Version": "3.7.1", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Gain": "1-HDR", "640 Shutter-TriggerSequenceLength": "1000", "528 AO-SupportsTriggering": "0", "445 AO-TriggerInputLine": "/Dev1/PFI0", "OpticalPath-Name": "OpticalPath", "PRIME-PP 4 ENABLED": "Yes", "PRIME-PVCAM Adapter Version": "1.3.29", "COM3-Name": "COM3", "405 AO-MaxVolts": "5.0000", "520 Shutter-Block voltage": "false", "PRIME-Timing-ClearingTimeNs": "49344000.0000", "Core-Focus": "PIEZO", "528 AO-Block voltage": "false", "TE2000-Description": "Nikon TE2000 hub", "528 AO-MinVolts": "0.0000", "COM4-Parity": "None", "Summary": { "Slices": 11, "ChContrastMin": [ 0, 0, 0, 0, 0, 0 ], "PixelAspect": 1, "SlicesFirst": true, "Interval_ms": 0, "Time": "2019-12-18 23:10:50 -0500", "Prefix": "seq_5", "InitialPositionList": [ { "Label": "Pos0", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.21], "Focus": [17741.5], "XYStage": [ 44542.200000000004, 31636.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos1", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.22], "Focus": [17741.4], "XYStage": [ 43801.100000000006, 31637.9 ] }, "GridColumnIndex": 0 }, { "Label": "Pos2", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.5], "XYStage": [ 38561.3, 32040.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos3", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.185], "Focus": [17741.3], "XYStage": [ 37698.4, 32041.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos4", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.16], "Focus": [17741.5], "XYStage": [ 32375.4, 31758.9 ] }, "GridColumnIndex": 0 }, { "Label": "Pos5", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.17000000000002], "Focus": [17741.3], "XYStage": [ 31770.7, 31758.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos6", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.4], "XYStage": [ 26536.800000000003, 31068.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos7", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.205], "Focus": [17741.4], "XYStage": [ 25420.2, 31068.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos8", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.85], "Focus": [17741.3], "XYStage": [ 21059, 31508 ] }, "GridColumnIndex": 0 }, { "Label": "Pos9", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.82], "Focus": [17741.3], "XYStage": [ 19731.9, 31506.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos10", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.79], "Focus": [17741.4], "XYStage": [ 14528.5, 32007.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos11", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.83], "Focus": [17741.2], "XYStage": [ 13830.300000000001, 32009.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos12", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.79], "Focus": [17741.3], "XYStage": [ 8775.300000000001, 32017.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos13", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.815], "Focus": [17741.3], "XYStage": [ 8109.200000000001, 32008.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos14", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.805], "Focus": [17741.3], "XYStage": [ 3483.3, 31908.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos15", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.82], "Focus": [17741.2], "XYStage": [ 2686.8, 31903.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos16", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [211.48000000000002], "Focus": [17741.1], "XYStage": [ -1978.9, 31973.600000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos17", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [211.51500000000001], "Focus": [17741.1], "XYStage": [ -2957.8, 31982.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos18", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.195], "Focus": [17741.5], "XYStage": [ 47747.4, -576.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos19", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.23000000000002], "Focus": [17741.6], "XYStage": [ 47053.9, -576.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos20", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.205], "Focus": [17741.4], "XYStage": [ 41871.200000000004, -479.8 ] }, "GridColumnIndex": 0 }, { "Label": "Pos21", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.4], "XYStage": [ 40882.600000000006, -392 ] }, "GridColumnIndex": 0 }, { "Label": "Pos22", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.24], "Focus": [17741.4], "XYStage": [ 35905.6, -135.3 ] }, "GridColumnIndex": 0 }, { "Label": "Pos23", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.195], "Focus": [17741.5], "XYStage": [ 34990.9, -132.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos24", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.46], "Focus": [17741.4], "XYStage": [ 29678.100000000002, -958.1 ] }, "GridColumnIndex": 0 }, { "Label": "Pos25", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.44], "Focus": [17741.5], "XYStage": [ 28983.600000000002, -939.4000000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos26", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.475], "Focus": [17741.5], "XYStage": [ 23019.7, -122 ] }, "GridColumnIndex": 0 }, { "Label": "Pos27", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.44], "Focus": [17741.7], "XYStage": [ 22428, -116.80000000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos28", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.46], "Focus": [17741.7], "XYStage": [ 16364.900000000001, -181.10000000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos29", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.48000000000002], "Focus": [17741.5], "XYStage": [ 15712.1, -180 ] }, "GridColumnIndex": 0 }, { "Label": "Pos30", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.45000000000002], "Focus": [17741.7], "XYStage": [ 10346.1, 77.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos31", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.49], "Focus": [17741.4], "XYStage": [ 9755.5, 85.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos32", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.475], "Focus": [17741.5], "XYStage": [ 4775.5, 293.90000000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos33", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.445], "Focus": [17741.4], "XYStage": [ 3954.9, 293.90000000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos34", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.455], "Focus": [17741.4], "XYStage": [ -1324.9, 545.6 ] }, "GridColumnIndex": 0 }, { "Label": "Pos35", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.445], "Focus": [17741.3], "XYStage": [ -2097, 576.6 ] }, "GridColumnIndex": 0 } ], "z-step_um": 10, "GridRow": 0, "BitDepth": 16, "Depth": 2, "Channels": 6, "Source": "Micro-Manager", "ChColors": [ -16711681, -256, -65281, -1, -1, -16776961 ], "MetadataVersion": 10, "KeepShutterOpenChannels": true, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536, 65536 ], "CustomIntervals_ms": [], "Height": 1200, "MicroManagerVersion": "1.4.23 20180219", "GridColumn": 0, "UUID": "67598d21-5db5-4842-ab60-f506f7b42c7c", "PixelType": "GRAY16", "Width": 1200, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\Yan\\20191218-YC113-S7S8-miseq", "Frames": 1, "Comment": "", "UserName": "Nikon", "PixelSize_um": 1.1, "ROI": [ 0, 0, 1200, 1200 ], "Date": "2019-12-18", "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC", "P-DAPI-high" ], "KeepShutterOpenSlices": true, "IJType": 1, "ComputerName": "ZadorConfocal2", "Positions": 36, "PositionIndex": 29, "CameraTimeout": 20000, "TimeFirst": false }, "640 AO-Name": "AnalogIO", "Vincent-D3-Command": "Close" }, "FrameKey-0-0-5": { "COM4-StopBits": "1", "528 AO-MaxVolts": "5.0000", "XLIGHT Excitation Wheel-State": "1", "470 Shutter-Name": "AnalogIO", "Core-Shutter": "520", "555 AO-Demo": "No", "XLIGHT Dichroic-State": "2", "XYStage-StepSize-Y": "0.1000", "ZPositionUm": 218.48000000000002, "XYStage-StepSize-X": "0.1000", "PRIME-FirmwareVersion": "20.28", "PRIME-ClearCycles": "2", "520-Name": "DA Shutter", "405 Shutter-Name": "AnalogIO", "PIEZO-StepSize": "0.0050", "PRIME-ClearMode": "Pre-Exposure", "470 AO-MinVolts": "0.0000", "405 AO-SupportsTriggering": "1", "CameraChannelIndex": 0, "XYStage-StartSpeed": "500.0000", "COM9-StopBits": "2", "405 AO-Description": "NI DAC", "COM4-DelayBetweenCharsMs": "0.0000", "445 AO-Block voltage": "false", "520 AO-TriggerInputLine": "/Dev1/PFI0", "555 AO-Description": "NI DAC", "520 AO-TriggerSampleRate": "100000", "COM4-Verbose": "1", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM4-Name": "COM4", "405 Shutter-Volts": "0.0000", "Vincent-D3-Port": "COM1", "PRIME-PP 4 MIN ADU AFFECTED": "200", "470 Shutter-MinVolts": "0.0000", "PRIME-PP 3 ENABLED": "Yes", "470 AO-Description": "NI DAC", "PRIME-Description": "PVCAM API device adapter", "520 Shutter-MaxVolts": "5.0000", "520 Shutter-Volts": "5.0000", "528 AO-Sequenceable": "1", "PRIME-Binning": "1x1", "555 Shutter-MaxVolts": "5.0000", "528 Shutter-TriggerSequenceLength": "1000", "405 Shutter-Block voltage": "false", "XLIGHT Spin motor-State": "1", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "Vincent-D3-Time to open (ms)": "35.0000", "Vincent-D3-Time to close (ms)": "35.0000", "445 AO-TriggerSampleRate": "100000", "640 Shutter-IOChannel": "Dev1/ao14", "555 AO-Sequenceable": "1", "520 AO-Name": "AnalogIO", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "470 AO-Block voltage": "false", "COM1-Verbose": "1", "COM9-AnswerTimeout": "10000.0000", "528 Shutter-Name": "AnalogIO", "PRIME-Trigger Timeout (secs)": "10", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "NextFrame": 0, "470 AO-TriggerSequenceLength": "1000", "PRIME-TransposeXY": "0", "445 AO-TriggerSequenceLength": "1000", "COM1-Name": "COM1", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "COM9-DataBits": "8", "COM1-AnswerTimeout": "500.0000", "Camera": "", "TE2000-Version": "V2.50-V2.50-V2.10", "528 Shutter-Volts": "0.0000", "XLIGHT Hub-Name": "XLIGHT Hub", "PIEZO-Description": "Ludl stage driver adapter", "PositionName": "Pos29", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSampleRate": "100000", "PRIME-FanSpeedSetpoint": "High", "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "405 AO-TriggerSampleRate": "100000", "470 Shutter-Block voltage": "false", "470-State": "0", "COM3-Parity": "None", "405 AO-Volts": "0.0000", "PRIME-TransposeMirrorY": "0", "PRIME-ChipName": "GS144BSI", "470 AO-Volts": "0.0000", "PRIME-TransposeMirrorX": "0", "445 Shutter-SupportsTriggering": "0", "470 Shutter-SupportsTriggering": "0", "XLIGHT Hub-Description": "XLIGHT hub", "PRIME-PreampOffLimit": "10000", "470 AO-Sequenceable": "1", "528 Shutter-MaxVolts": "5.0000", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "Core-XYStage": "XYStage", "470 AO-TriggerSampleRate": "100000", "PRIME-Timing-PreTriggerDelayNs": "24672000.0000", "528-DA Device": "528 Shutter", "Focus-Description": "Nikon TE2000 focus adapter", "FilterBlock-State": "5", "520 AO-Demo": "No", "COM3-DelayBetweenCharsMs": "0.0000", "470 Shutter-Volts": "0.0000", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "PRIME-PP 2 THRESHOLD": "125", "PRIME-PP 4 THRESHOLD": "75", "XYStage-Acceleration": "4.0000", "470 Shutter-Demo": "No", "PRIME-Name": "PMPCIECam00", "640-DA Device": "640 Shutter", "405 AO-Block voltage": "false", "ElapsedTime-ms": 1193176, "PRIME-Offset": "100", "445 AO-IOChannel": "Dev1/ao1", "640 AO-MaxVolts": "5.0000", "PositionIndex": 29, "PRIME-PP 1 ENABLED": "Yes", "COM1-BaudRate": "9600", "640-State": "0", "COM3-Verbose": "1", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "LudlController-TransmissionDelay": "0", "PRIME-FullWellCapacity": "62000", "PRIME-SMARTStreamingEnabled": "No", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "640 Shutter-Description": "NI DAC", "528-Description": "DA device that is used as a shutter", "COM4-Description": "Serial port driver (boost:asio)", "405 Shutter-Description": "NI DAC", "TE2000-Name": "TE2000", "528 Shutter-Description": "NI DAC", "COM9-Description": "Serial port driver (boost:asio)", "LudlController-Name": "LudlController", "445 Shutter-TriggerSequenceLength": "1000", "PRIME-SerialNumber": "A18B203008", "445 AO-Volts": "0.0000", "PRIME-X-dimension": "1200", "PRIME-ShutterCloseDelay": "0", "XLIGHT Disk slider-State": "2", "Position": "Default", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 AO-IOChannel": "Dev1/ao5", "520 AO-IOChannel": "Dev1/ao4", "528 AO-TriggerSequenceLength": "1000", "405 Shutter-MaxVolts": "5.0000", "470-DA Device": "470 Shutter", "PRIME-FTCapable": "No", "Core-ChannelGroup": "Channels", "555 AO-SupportsTriggering": "0", "PRIME-Y-dimension": "1200", "Frame": 0, "555 Shutter-Sequenceable": "1", "PRIME-Timing-ExposureTimeNs": "499998000.0000", "Core-ImageProcessor": "", "528-State": "0", "520 AO-Block voltage": "false", "XLIGHT Excitation Wheel-Label": "CFP/YFP", "640 Shutter-Demo": "No", "COM3-StopBits": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "520 Shutter-IOChannel": "Dev1/ao11", "Width": 1200, "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-SupportsTriggering": "0", "640 AO-TriggerSampleRate": "100000", "520-Description": "DA device that is used as a shutter", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "405 Shutter-Sequenceable": "1", "405 Shutter-SupportsTriggering": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-TriggerSampleRate": "100000", "640 Shutter-MaxVolts": "5.0000", "555 AO-MaxVolts": "5.0000", "520 AO-TriggerSequenceLength": "1000", "COM3-BaudRate": "9600", "COM9-BaudRate": "9600", "Vincent-D3-Channel #": "Ch. #1", "PRIME-PMode": "Normal", "OpticalPath-Label": "Left(100)", "BitDepth": 16, "555 AO-TriggerSequenceLength": "1000", "445 Shutter-Description": "NI DAC", "405 AO-Sequenceable": "1", "PRIME-AcquisitionMethod": "Callbacks", "555 AO-Name": "AnalogIO", "PRIME-ShutterMode": "Pre-Exposure", "COM3-Handshaking": "Off", "445 AO-SupportsTriggering": "0", "ROI": "0-0-1200-1200", "COM9-Verbose": "1", "PRIME-Color": "OFF", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "PRIME-ShutterOpenDelay": "0", "COM1-Parity": "None", "Slice": 5, "SlicePosition": 0, "COM9-DelayBetweenCharsMs": "0.0000", "555-Name": "DA Shutter", "PIEZO-LudlSingleAxisName": "B", "XLIGHT Emission Wheel-Label": "565/24(G)", "640 Shutter-Name": "AnalogIO", "640 AO-Sequenceable": "1", "520 Shutter-Demo": "No", "528 Shutter-Demo": "No", "470-Name": "DA Shutter", "520-DA Device": "520 Shutter", "XYStage-Description": "Ludl XY stage driver adapter", "445 Shutter-MinVolts": "0.0000", "528 AO-TriggerSampleRate": "100000", "405 AO-MinVolts": "0.0000", "405 Shutter-TriggerSequenceLength": "1000", "Height": 1200, "555 AO-Block voltage": "false", "640 Shutter-Volts": "0.0000", "PixelType": "GRAY16", "445 Shutter-Block voltage": "false", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "520 Shutter-TriggerSequenceLength": "1000", "COM9-Name": "COM9", "Core-SLM": "", "555 AO-Volts": "0.0000", "555 Shutter-Block voltage": "false", "XYStage-Speed": "1800.0000", "520 AO-MaxVolts": "5.0000", "528 Shutter-SupportsTriggering": "0", "COM1-Description": "Serial port driver (boost:asio)", "PRIME-MetadataEnabled": "No", "520 Shutter-MinVolts": "0.0000", "470 Shutter-Sequenceable": "1", "PRIME-PreampDelay": "0", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-MinVolts": "0.0000", "ChannelIndex": 0, "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Core-AutoShutter": "1", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "Exposure-ms": 320, "PRIME-PP 2 ENABLED": "Yes", "640 Shutter-TriggerSampleRate": "100000", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "470 Shutter-IOChannel": "Dev1/ao10", "405 AO-Demo": "No", "PixelSizeUm": 1.1, "470 AO-Demo": "No", "470 Shutter-MaxVolts": "5.0000", "Vincent-D3-Name": "Vincent-D3", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FileName": "img_000000000_Il-G_005.tif", "LudlController-Reset": "Operate", "PRIME-Exposure": "320.00", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "445-Name": "DA Shutter", "555 Shutter-Name": "AnalogIO", "520 Shutter-TriggerSampleRate": "100000", "445 Shutter-IOChannel": "Dev1/ao9", "PRIME-TransposeCorrection": "0", "XYStage-StepSize": "0.1000", "445-Description": "DA device that is used as a shutter", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 AO-IOChannel": "Dev1/ao7", "520 Shutter-Name": "AnalogIO", "COM3-DataBits": "8", "640 AO-Volts": "0.0000", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "555 Shutter-IOChannel": "Dev1/ao13", "WaitInterval": null, "520 Shutter-Sequenceable": "1", "YPositionUm": -180, "445-State": "0", "445-DA Device": "445 Shutter", "PRIME-CircularBufferAutoSize": "ON", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "555 AO-TriggerSampleRate": "100000", "445 Shutter-Volts": "0.0000", "XLIGHT Hub-Port": "COM3", "Core-AutoFocus": "", "COM4-Handshaking": "Off", "528 Shutter-MinVolts": "0.0000", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "445 Shutter-Name": "AnalogIO", "528 Shutter-Sequenceable": "1", "XYStage-TransposeMirrorX": "0", "XYStage-TransposeMirrorY": "0", "PRIME-ReadoutRate": "100MHz 16bit", "XYStage-Name": "Stage", "640 AO-SupportsTriggering": "0", "640-Name": "DA Shutter", "640 Shutter-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "640 AO-Block voltage": "false", "445 Shutter-Sequenceable": "1", "470 AO-MaxVolts": "5.0000", "COM4-BaudRate": "9600", "470-Description": "DA device that is used as a shutter", "520 Shutter-Description": "NI DAC", "PRIME-CCDTemperature": "-14.8500", "Time": "2019-12-18 23:10:53 -0500", "640 AO-TriggerSequenceLength": "1000", "470 AO-Name": "AnalogIO", "XLIGHT Disk slider-Label": "Disk Pos 40um", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "Binning": "1x1", "TE2000-ModelType": "1", "COM3-AnswerTimeout": "500.0000", "555 Shutter-SupportsTriggering": "0", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "TE2000-Port": "COM4", "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Description": "NI DAC", "COM4-AnswerTimeout": "500.0000", "COM1-DelayBetweenCharsMs": "0.0000", "FilterBlock-Label": "Confocal", "445 AO-Name": "AnalogIO", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-Timing-ReadoutTimeNs": "24672000.0000", "528 AO-Demo": "No", "520 AO-Sequenceable": "1", "555-Description": "DA device that is used as a shutter", "405-DA Device": "405 Shutter", "405 AO-TriggerSequenceLength": "1000", "640 AO-MinVolts": "0.0000", "640 Shutter-Block voltage": "false", "FrameIndex": 0, "PRIME-Port": "CMOS", "555 AO-MinVolts": "0.0000", "COM3-Description": "Serial port driver (boost:asio)", "528 AO-Description": "NI DAC", "555 AO-IOChannel": "Dev1/ao6", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "555 AO-TriggerInputLine": "/Dev1/PFI0", "PRIME-ActualInterval-ms": "390.5200", "LudlController-Port": "COM9", "445 Shutter-Demo": "No", "PRIME-Trigger-Expose Out-Mux": "1", "SliceIndex": 5, "PRIME-CCDTemperatureSetPoint": "-15.0000", "405 Shutter-MinVolts": "0.0000", "PRIME-ExposeOutMode": "First Row", "AxisPositions": null, "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Volts": "0.0000", "528-Name": "DA Shutter", "555-State": "0", "XLIGHT Dichroic-Label": "CFP/YFP", "445 AO-Sequenceable": "1", "405-Name": "DA Shutter", "405 AO-IOChannel": "Dev1/ao0", "Core-Camera": "PRIME", "Focus-Name": "Focus", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "405 Shutter-IOChannel": "Dev1/ao8", "640 AO-Demo": "No", "640 Shutter-SupportsTriggering": "0", "445 AO-Demo": "No", "555-DA Device": "555 Shutter", "470 Shutter-Description": "NI DAC", "528 AO-Volts": "0.0000", "COM1-DataBits": "8", "Vincent-D3-Address": "x", "520 AO-SupportsTriggering": "0", "405-Description": "DA device that is used as a shutter", "640 Shutter-MinVolts": "0.0000", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "405 AO-Name": "AnalogIO", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "640 AO-TriggerInputLine": "/Dev1/PFI0", "405 AO-TriggerInputLine": "/Dev1/PFI0", "640-Description": "DA device that is used as a shutter", "445 Shutter-TriggerSampleRate": "100000", "470 Shutter-TriggerSequenceLength": "1000", "COM1-StopBits": "1", "520 Shutter-SupportsTriggering": "0", "405-State": "0", "Core-Initialize": "1", "XLIGHT Emission Wheel-State": "3", "520 AO-Description": "NI DAC", "528 AO-TriggerInputLine": "/Dev1/PFI0", "445 Shutter-MaxVolts": "5.0000", "COM9-Handshaking": "Off", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "XLIGHT Spin motor-Label": "On (spinning)", "PRIME-PP 5 ENABLED": "No", "UUID": "dea20cf2-431a-4458-a22d-3e778dac8941", "PRIME-TriggerMode": "Internal Trigger", "COM1-Handshaking": "Off", "PRIME-Timing-PostTriggerDelayNs": "49344000.0000", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "Focus-Position": "17741.5000", "528 Shutter-Block voltage": "false", "Channel": "Il-G", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "555 Shutter-Demo": "No", "405 Shutter-Demo": "No", "528 AO-Name": "AnalogIO", "520-State": "1", "Core-Galvo": "", "405 Shutter-TriggerSampleRate": "100000", "Focus-FocusStepSize_(nm)": "100", "PRIME-PixelType": "16bit", "COM4-DataBits": "8", "470 AO-IOChannel": "Dev1/ao2", "520 AO-MinVolts": "0.0000", "COM9-Parity": "None", "PRIME-CircularBufferEnabled": "ON", "PRIME-PP 6 Reset": "No", "640 AO-Description": "NI DAC", "Source": "PRIME", "445 AO-MinVolts": "0.0000", "PIEZO-Name": "Stage", "445 AO-Description": "NI DAC", "PRIME-CircularBufferFrameCount": "46", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "520 AO-Volts": "4.5000", "528 Shutter-TriggerSampleRate": "100000", "XPositionUm": 15712.1, "OpticalPath-State": "4", "PRIME-PP 3 THRESHOLD": "80", "PRIME-PVCAM Version": "3.7.1", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Gain": "1-HDR", "640 Shutter-TriggerSequenceLength": "1000", "528 AO-SupportsTriggering": "0", "445 AO-TriggerInputLine": "/Dev1/PFI0", "OpticalPath-Name": "OpticalPath", "PRIME-PP 4 ENABLED": "Yes", "PRIME-PVCAM Adapter Version": "1.3.29", "COM3-Name": "COM3", "405 AO-MaxVolts": "5.0000", "520 Shutter-Block voltage": "false", "PRIME-Timing-ClearingTimeNs": "49344000.0000", "Core-Focus": "PIEZO", "528 AO-Block voltage": "false", "TE2000-Description": "Nikon TE2000 hub", "528 AO-MinVolts": "0.0000", "COM4-Parity": "None", "Summary": { "Slices": 11, "ChContrastMin": [ 0, 0, 0, 0, 0, 0 ], "PixelAspect": 1, "SlicesFirst": true, "Interval_ms": 0, "Time": "2019-12-18 23:10:50 -0500", "Prefix": "seq_5", "InitialPositionList": [ { "Label": "Pos0", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.21], "Focus": [17741.5], "XYStage": [ 44542.200000000004, 31636.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos1", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.22], "Focus": [17741.4], "XYStage": [ 43801.100000000006, 31637.9 ] }, "GridColumnIndex": 0 }, { "Label": "Pos2", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.5], "XYStage": [ 38561.3, 32040.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos3", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.185], "Focus": [17741.3], "XYStage": [ 37698.4, 32041.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos4", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.16], "Focus": [17741.5], "XYStage": [ 32375.4, 31758.9 ] }, "GridColumnIndex": 0 }, { "Label": "Pos5", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.17000000000002], "Focus": [17741.3], "XYStage": [ 31770.7, 31758.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos6", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.4], "XYStage": [ 26536.800000000003, 31068.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos7", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.205], "Focus": [17741.4], "XYStage": [ 25420.2, 31068.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos8", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.85], "Focus": [17741.3], "XYStage": [ 21059, 31508 ] }, "GridColumnIndex": 0 }, { "Label": "Pos9", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.82], "Focus": [17741.3], "XYStage": [ 19731.9, 31506.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos10", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.79], "Focus": [17741.4], "XYStage": [ 14528.5, 32007.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos11", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.83], "Focus": [17741.2], "XYStage": [ 13830.300000000001, 32009.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos12", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.79], "Focus": [17741.3], "XYStage": [ 8775.300000000001, 32017.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos13", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.815], "Focus": [17741.3], "XYStage": [ 8109.200000000001, 32008.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos14", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.805], "Focus": [17741.3], "XYStage": [ 3483.3, 31908.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos15", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.82], "Focus": [17741.2], "XYStage": [ 2686.8, 31903.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos16", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [211.48000000000002], "Focus": [17741.1], "XYStage": [ -1978.9, 31973.600000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos17", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [211.51500000000001], "Focus": [17741.1], "XYStage": [ -2957.8, 31982.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos18", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.195], "Focus": [17741.5], "XYStage": [ 47747.4, -576.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos19", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.23000000000002], "Focus": [17741.6], "XYStage": [ 47053.9, -576.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos20", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.205], "Focus": [17741.4], "XYStage": [ 41871.200000000004, -479.8 ] }, "GridColumnIndex": 0 }, { "Label": "Pos21", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.4], "XYStage": [ 40882.600000000006, -392 ] }, "GridColumnIndex": 0 }, { "Label": "Pos22", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.24], "Focus": [17741.4], "XYStage": [ 35905.6, -135.3 ] }, "GridColumnIndex": 0 }, { "Label": "Pos23", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.195], "Focus": [17741.5], "XYStage": [ 34990.9, -132.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos24", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.46], "Focus": [17741.4], "XYStage": [ 29678.100000000002, -958.1 ] }, "GridColumnIndex": 0 }, { "Label": "Pos25", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.44], "Focus": [17741.5], "XYStage": [ 28983.600000000002, -939.4000000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos26", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.475], "Focus": [17741.5], "XYStage": [ 23019.7, -122 ] }, "GridColumnIndex": 0 }, { "Label": "Pos27", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.44], "Focus": [17741.7], "XYStage": [ 22428, -116.80000000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos28", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.46], "Focus": [17741.7], "XYStage": [ 16364.900000000001, -181.10000000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos29", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.48000000000002], "Focus": [17741.5], "XYStage": [ 15712.1, -180 ] }, "GridColumnIndex": 0 }, { "Label": "Pos30", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.45000000000002], "Focus": [17741.7], "XYStage": [ 10346.1, 77.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos31", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.49], "Focus": [17741.4], "XYStage": [ 9755.5, 85.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos32", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.475], "Focus": [17741.5], "XYStage": [ 4775.5, 293.90000000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos33", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.445], "Focus": [17741.4], "XYStage": [ 3954.9, 293.90000000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos34", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.455], "Focus": [17741.4], "XYStage": [ -1324.9, 545.6 ] }, "GridColumnIndex": 0 }, { "Label": "Pos35", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.445], "Focus": [17741.3], "XYStage": [ -2097, 576.6 ] }, "GridColumnIndex": 0 } ], "z-step_um": 10, "GridRow": 0, "BitDepth": 16, "Depth": 2, "Channels": 6, "Source": "Micro-Manager", "ChColors": [ -16711681, -256, -65281, -1, -1, -16776961 ], "MetadataVersion": 10, "KeepShutterOpenChannels": true, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536, 65536 ], "CustomIntervals_ms": [], "Height": 1200, "MicroManagerVersion": "1.4.23 20180219", "GridColumn": 0, "UUID": "67598d21-5db5-4842-ab60-f506f7b42c7c", "PixelType": "GRAY16", "Width": 1200, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\Yan\\20191218-YC113-S7S8-miseq", "Frames": 1, "Comment": "", "UserName": "Nikon", "PixelSize_um": 1.1, "ROI": [ 0, 0, 1200, 1200 ], "Date": "2019-12-18", "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC", "P-DAPI-high" ], "KeepShutterOpenSlices": true, "IJType": 1, "ComputerName": "ZadorConfocal2", "Positions": 36, "PositionIndex": 29, "CameraTimeout": 20000, "TimeFirst": false }, "640 AO-Name": "AnalogIO", "Vincent-D3-Command": "Close" }, "FrameKey-0-0-6": { "COM4-StopBits": "1", "528 AO-MaxVolts": "5.0000", "XLIGHT Excitation Wheel-State": "1", "470 Shutter-Name": "AnalogIO", "Core-Shutter": "520", "555 AO-Demo": "No", "XLIGHT Dichroic-State": "2", "XYStage-StepSize-Y": "0.1000", "ZPositionUm": 228.48000000000002, "XYStage-StepSize-X": "0.1000", "PRIME-FirmwareVersion": "20.28", "PRIME-ClearCycles": "2", "520-Name": "DA Shutter", "405 Shutter-Name": "AnalogIO", "PIEZO-StepSize": "0.0050", "PRIME-ClearMode": "Pre-Exposure", "470 AO-MinVolts": "0.0000", "405 AO-SupportsTriggering": "1", "CameraChannelIndex": 0, "XYStage-StartSpeed": "500.0000", "COM9-StopBits": "2", "405 AO-Description": "NI DAC", "COM4-DelayBetweenCharsMs": "0.0000", "445 AO-Block voltage": "false", "520 AO-TriggerInputLine": "/Dev1/PFI0", "555 AO-Description": "NI DAC", "520 AO-TriggerSampleRate": "100000", "COM4-Verbose": "1", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM4-Name": "COM4", "405 Shutter-Volts": "0.0000", "Vincent-D3-Port": "COM1", "PRIME-PP 4 MIN ADU AFFECTED": "200", "470 Shutter-MinVolts": "0.0000", "PRIME-PP 3 ENABLED": "Yes", "470 AO-Description": "NI DAC", "PRIME-Description": "PVCAM API device adapter", "520 Shutter-MaxVolts": "5.0000", "520 Shutter-Volts": "5.0000", "528 AO-Sequenceable": "1", "PRIME-Binning": "1x1", "555 Shutter-MaxVolts": "5.0000", "528 Shutter-TriggerSequenceLength": "1000", "405 Shutter-Block voltage": "false", "XLIGHT Spin motor-State": "1", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "Vincent-D3-Time to open (ms)": "35.0000", "Vincent-D3-Time to close (ms)": "35.0000", "445 AO-TriggerSampleRate": "100000", "640 Shutter-IOChannel": "Dev1/ao14", "555 AO-Sequenceable": "1", "520 AO-Name": "AnalogIO", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "470 AO-Block voltage": "false", "COM1-Verbose": "1", "COM9-AnswerTimeout": "10000.0000", "528 Shutter-Name": "AnalogIO", "PRIME-Trigger Timeout (secs)": "10", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "NextFrame": 0, "470 AO-TriggerSequenceLength": "1000", "PRIME-TransposeXY": "0", "445 AO-TriggerSequenceLength": "1000", "COM1-Name": "COM1", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "COM9-DataBits": "8", "COM1-AnswerTimeout": "500.0000", "Camera": "", "TE2000-Version": "V2.50-V2.50-V2.10", "528 Shutter-Volts": "0.0000", "XLIGHT Hub-Name": "XLIGHT Hub", "PIEZO-Description": "Ludl stage driver adapter", "PositionName": "Pos29", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSampleRate": "100000", "PRIME-FanSpeedSetpoint": "High", "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "405 AO-TriggerSampleRate": "100000", "470 Shutter-Block voltage": "false", "470-State": "0", "COM3-Parity": "None", "405 AO-Volts": "0.0000", "PRIME-TransposeMirrorY": "0", "PRIME-ChipName": "GS144BSI", "470 AO-Volts": "0.0000", "PRIME-TransposeMirrorX": "0", "445 Shutter-SupportsTriggering": "0", "470 Shutter-SupportsTriggering": "0", "XLIGHT Hub-Description": "XLIGHT hub", "PRIME-PreampOffLimit": "10000", "470 AO-Sequenceable": "1", "528 Shutter-MaxVolts": "5.0000", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "Core-XYStage": "XYStage", "470 AO-TriggerSampleRate": "100000", "PRIME-Timing-PreTriggerDelayNs": "24672000.0000", "528-DA Device": "528 Shutter", "Focus-Description": "Nikon TE2000 focus adapter", "FilterBlock-State": "5", "520 AO-Demo": "No", "COM3-DelayBetweenCharsMs": "0.0000", "470 Shutter-Volts": "0.0000", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "PRIME-PP 2 THRESHOLD": "125", "PRIME-PP 4 THRESHOLD": "75", "XYStage-Acceleration": "4.0000", "470 Shutter-Demo": "No", "PRIME-Name": "PMPCIECam00", "640-DA Device": "640 Shutter", "405 AO-Block voltage": "false", "ElapsedTime-ms": 1193735, "PRIME-Offset": "100", "445 AO-IOChannel": "Dev1/ao1", "640 AO-MaxVolts": "5.0000", "PositionIndex": 29, "PRIME-PP 1 ENABLED": "Yes", "COM1-BaudRate": "9600", "640-State": "0", "COM3-Verbose": "1", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "LudlController-TransmissionDelay": "0", "PRIME-FullWellCapacity": "62000", "PRIME-SMARTStreamingEnabled": "No", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "640 Shutter-Description": "NI DAC", "528-Description": "DA device that is used as a shutter", "COM4-Description": "Serial port driver (boost:asio)", "405 Shutter-Description": "NI DAC", "TE2000-Name": "TE2000", "528 Shutter-Description": "NI DAC", "COM9-Description": "Serial port driver (boost:asio)", "LudlController-Name": "LudlController", "445 Shutter-TriggerSequenceLength": "1000", "PRIME-SerialNumber": "A18B203008", "445 AO-Volts": "0.0000", "PRIME-X-dimension": "1200", "PRIME-ShutterCloseDelay": "0", "XLIGHT Disk slider-State": "2", "Position": "Default", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 AO-IOChannel": "Dev1/ao5", "520 AO-IOChannel": "Dev1/ao4", "528 AO-TriggerSequenceLength": "1000", "405 Shutter-MaxVolts": "5.0000", "470-DA Device": "470 Shutter", "PRIME-FTCapable": "No", "Core-ChannelGroup": "Channels", "555 AO-SupportsTriggering": "0", "PRIME-Y-dimension": "1200", "Frame": 0, "555 Shutter-Sequenceable": "1", "PRIME-Timing-ExposureTimeNs": "499998000.0000", "Core-ImageProcessor": "", "528-State": "0", "520 AO-Block voltage": "false", "XLIGHT Excitation Wheel-Label": "CFP/YFP", "640 Shutter-Demo": "No", "COM3-StopBits": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "520 Shutter-IOChannel": "Dev1/ao11", "Width": 1200, "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-SupportsTriggering": "0", "640 AO-TriggerSampleRate": "100000", "520-Description": "DA device that is used as a shutter", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "405 Shutter-Sequenceable": "1", "405 Shutter-SupportsTriggering": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-TriggerSampleRate": "100000", "640 Shutter-MaxVolts": "5.0000", "555 AO-MaxVolts": "5.0000", "520 AO-TriggerSequenceLength": "1000", "COM3-BaudRate": "9600", "COM9-BaudRate": "9600", "Vincent-D3-Channel #": "Ch. #1", "PRIME-PMode": "Normal", "OpticalPath-Label": "Left(100)", "BitDepth": 16, "555 AO-TriggerSequenceLength": "1000", "445 Shutter-Description": "NI DAC", "405 AO-Sequenceable": "1", "PRIME-AcquisitionMethod": "Callbacks", "555 AO-Name": "AnalogIO", "PRIME-ShutterMode": "Pre-Exposure", "COM3-Handshaking": "Off", "445 AO-SupportsTriggering": "0", "ROI": "0-0-1200-1200", "COM9-Verbose": "1", "PRIME-Color": "OFF", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "PRIME-ShutterOpenDelay": "0", "COM1-Parity": "None", "Slice": 6, "SlicePosition": 10, "COM9-DelayBetweenCharsMs": "0.0000", "555-Name": "DA Shutter", "PIEZO-LudlSingleAxisName": "B", "XLIGHT Emission Wheel-Label": "565/24(G)", "640 Shutter-Name": "AnalogIO", "640 AO-Sequenceable": "1", "520 Shutter-Demo": "No", "528 Shutter-Demo": "No", "470-Name": "DA Shutter", "520-DA Device": "520 Shutter", "XYStage-Description": "Ludl XY stage driver adapter", "445 Shutter-MinVolts": "0.0000", "528 AO-TriggerSampleRate": "100000", "405 AO-MinVolts": "0.0000", "405 Shutter-TriggerSequenceLength": "1000", "Height": 1200, "555 AO-Block voltage": "false", "640 Shutter-Volts": "0.0000", "PixelType": "GRAY16", "445 Shutter-Block voltage": "false", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "520 Shutter-TriggerSequenceLength": "1000", "COM9-Name": "COM9", "Core-SLM": "", "555 AO-Volts": "0.0000", "555 Shutter-Block voltage": "false", "XYStage-Speed": "1800.0000", "520 AO-MaxVolts": "5.0000", "528 Shutter-SupportsTriggering": "0", "COM1-Description": "Serial port driver (boost:asio)", "PRIME-MetadataEnabled": "No", "520 Shutter-MinVolts": "0.0000", "470 Shutter-Sequenceable": "1", "PRIME-PreampDelay": "0", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-MinVolts": "0.0000", "ChannelIndex": 0, "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Core-AutoShutter": "1", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "Exposure-ms": 320, "PRIME-PP 2 ENABLED": "Yes", "640 Shutter-TriggerSampleRate": "100000", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "470 Shutter-IOChannel": "Dev1/ao10", "405 AO-Demo": "No", "PixelSizeUm": 1.1, "470 AO-Demo": "No", "470 Shutter-MaxVolts": "5.0000", "Vincent-D3-Name": "Vincent-D3", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FileName": "img_000000000_Il-G_006.tif", "LudlController-Reset": "Operate", "PRIME-Exposure": "320.00", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "445-Name": "DA Shutter", "555 Shutter-Name": "AnalogIO", "520 Shutter-TriggerSampleRate": "100000", "445 Shutter-IOChannel": "Dev1/ao9", "PRIME-TransposeCorrection": "0", "XYStage-StepSize": "0.1000", "445-Description": "DA device that is used as a shutter", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 AO-IOChannel": "Dev1/ao7", "520 Shutter-Name": "AnalogIO", "COM3-DataBits": "8", "640 AO-Volts": "0.0000", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "555 Shutter-IOChannel": "Dev1/ao13", "WaitInterval": null, "520 Shutter-Sequenceable": "1", "YPositionUm": -180, "445-State": "0", "445-DA Device": "445 Shutter", "PRIME-CircularBufferAutoSize": "ON", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "555 AO-TriggerSampleRate": "100000", "445 Shutter-Volts": "0.0000", "XLIGHT Hub-Port": "COM3", "Core-AutoFocus": "", "COM4-Handshaking": "Off", "528 Shutter-MinVolts": "0.0000", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "445 Shutter-Name": "AnalogIO", "528 Shutter-Sequenceable": "1", "XYStage-TransposeMirrorX": "0", "XYStage-TransposeMirrorY": "0", "PRIME-ReadoutRate": "100MHz 16bit", "XYStage-Name": "Stage", "640 AO-SupportsTriggering": "0", "640-Name": "DA Shutter", "640 Shutter-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "640 AO-Block voltage": "false", "445 Shutter-Sequenceable": "1", "470 AO-MaxVolts": "5.0000", "COM4-BaudRate": "9600", "470-Description": "DA device that is used as a shutter", "520 Shutter-Description": "NI DAC", "PRIME-CCDTemperature": "-14.8500", "Time": "2019-12-18 23:10:53 -0500", "640 AO-TriggerSequenceLength": "1000", "470 AO-Name": "AnalogIO", "XLIGHT Disk slider-Label": "Disk Pos 40um", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "Binning": "1x1", "TE2000-ModelType": "1", "COM3-AnswerTimeout": "500.0000", "555 Shutter-SupportsTriggering": "0", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "TE2000-Port": "COM4", "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Description": "NI DAC", "COM4-AnswerTimeout": "500.0000", "COM1-DelayBetweenCharsMs": "0.0000", "FilterBlock-Label": "Confocal", "445 AO-Name": "AnalogIO", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-Timing-ReadoutTimeNs": "24672000.0000", "528 AO-Demo": "No", "520 AO-Sequenceable": "1", "555-Description": "DA device that is used as a shutter", "405-DA Device": "405 Shutter", "405 AO-TriggerSequenceLength": "1000", "640 AO-MinVolts": "0.0000", "640 Shutter-Block voltage": "false", "FrameIndex": 0, "PRIME-Port": "CMOS", "555 AO-MinVolts": "0.0000", "COM3-Description": "Serial port driver (boost:asio)", "528 AO-Description": "NI DAC", "555 AO-IOChannel": "Dev1/ao6", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "555 AO-TriggerInputLine": "/Dev1/PFI0", "PRIME-ActualInterval-ms": "390.5200", "LudlController-Port": "COM9", "445 Shutter-Demo": "No", "PRIME-Trigger-Expose Out-Mux": "1", "SliceIndex": 6, "PRIME-CCDTemperatureSetPoint": "-15.0000", "405 Shutter-MinVolts": "0.0000", "PRIME-ExposeOutMode": "First Row", "AxisPositions": null, "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Volts": "0.0000", "528-Name": "DA Shutter", "555-State": "0", "XLIGHT Dichroic-Label": "CFP/YFP", "445 AO-Sequenceable": "1", "405-Name": "DA Shutter", "405 AO-IOChannel": "Dev1/ao0", "Core-Camera": "PRIME", "Focus-Name": "Focus", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "405 Shutter-IOChannel": "Dev1/ao8", "640 AO-Demo": "No", "640 Shutter-SupportsTriggering": "0", "445 AO-Demo": "No", "555-DA Device": "555 Shutter", "470 Shutter-Description": "NI DAC", "528 AO-Volts": "0.0000", "COM1-DataBits": "8", "Vincent-D3-Address": "x", "520 AO-SupportsTriggering": "0", "405-Description": "DA device that is used as a shutter", "640 Shutter-MinVolts": "0.0000", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "405 AO-Name": "AnalogIO", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "640 AO-TriggerInputLine": "/Dev1/PFI0", "405 AO-TriggerInputLine": "/Dev1/PFI0", "640-Description": "DA device that is used as a shutter", "445 Shutter-TriggerSampleRate": "100000", "470 Shutter-TriggerSequenceLength": "1000", "COM1-StopBits": "1", "520 Shutter-SupportsTriggering": "0", "405-State": "0", "Core-Initialize": "1", "XLIGHT Emission Wheel-State": "3", "520 AO-Description": "NI DAC", "528 AO-TriggerInputLine": "/Dev1/PFI0", "445 Shutter-MaxVolts": "5.0000", "COM9-Handshaking": "Off", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "XLIGHT Spin motor-Label": "On (spinning)", "PRIME-PP 5 ENABLED": "No", "UUID": "118e81f1-fb01-42fb-90d0-4e581adfa5fc", "PRIME-TriggerMode": "Internal Trigger", "COM1-Handshaking": "Off", "PRIME-Timing-PostTriggerDelayNs": "49344000.0000", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "Focus-Position": "17741.5000", "528 Shutter-Block voltage": "false", "Channel": "Il-G", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "555 Shutter-Demo": "No", "405 Shutter-Demo": "No", "528 AO-Name": "AnalogIO", "520-State": "1", "Core-Galvo": "", "405 Shutter-TriggerSampleRate": "100000", "Focus-FocusStepSize_(nm)": "100", "PRIME-PixelType": "16bit", "COM4-DataBits": "8", "470 AO-IOChannel": "Dev1/ao2", "520 AO-MinVolts": "0.0000", "COM9-Parity": "None", "PRIME-CircularBufferEnabled": "ON", "PRIME-PP 6 Reset": "No", "640 AO-Description": "NI DAC", "Source": "PRIME", "445 AO-MinVolts": "0.0000", "PIEZO-Name": "Stage", "445 AO-Description": "NI DAC", "PRIME-CircularBufferFrameCount": "46", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "520 AO-Volts": "4.5000", "528 Shutter-TriggerSampleRate": "100000", "XPositionUm": 15712.1, "OpticalPath-State": "4", "PRIME-PP 3 THRESHOLD": "80", "PRIME-PVCAM Version": "3.7.1", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Gain": "1-HDR", "640 Shutter-TriggerSequenceLength": "1000", "528 AO-SupportsTriggering": "0", "445 AO-TriggerInputLine": "/Dev1/PFI0", "OpticalPath-Name": "OpticalPath", "PRIME-PP 4 ENABLED": "Yes", "PRIME-PVCAM Adapter Version": "1.3.29", "COM3-Name": "COM3", "405 AO-MaxVolts": "5.0000", "520 Shutter-Block voltage": "false", "PRIME-Timing-ClearingTimeNs": "49344000.0000", "Core-Focus": "PIEZO", "528 AO-Block voltage": "false", "TE2000-Description": "Nikon TE2000 hub", "528 AO-MinVolts": "0.0000", "COM4-Parity": "None", "Summary": { "Slices": 11, "ChContrastMin": [ 0, 0, 0, 0, 0, 0 ], "PixelAspect": 1, "SlicesFirst": true, "Interval_ms": 0, "Time": "2019-12-18 23:10:50 -0500", "Prefix": "seq_5", "InitialPositionList": [ { "Label": "Pos0", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.21], "Focus": [17741.5], "XYStage": [ 44542.200000000004, 31636.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos1", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.22], "Focus": [17741.4], "XYStage": [ 43801.100000000006, 31637.9 ] }, "GridColumnIndex": 0 }, { "Label": "Pos2", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.5], "XYStage": [ 38561.3, 32040.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos3", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.185], "Focus": [17741.3], "XYStage": [ 37698.4, 32041.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos4", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.16], "Focus": [17741.5], "XYStage": [ 32375.4, 31758.9 ] }, "GridColumnIndex": 0 }, { "Label": "Pos5", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.17000000000002], "Focus": [17741.3], "XYStage": [ 31770.7, 31758.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos6", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.4], "XYStage": [ 26536.800000000003, 31068.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos7", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.205], "Focus": [17741.4], "XYStage": [ 25420.2, 31068.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos8", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.85], "Focus": [17741.3], "XYStage": [ 21059, 31508 ] }, "GridColumnIndex": 0 }, { "Label": "Pos9", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.82], "Focus": [17741.3], "XYStage": [ 19731.9, 31506.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos10", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.79], "Focus": [17741.4], "XYStage": [ 14528.5, 32007.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos11", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.83], "Focus": [17741.2], "XYStage": [ 13830.300000000001, 32009.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos12", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.79], "Focus": [17741.3], "XYStage": [ 8775.300000000001, 32017.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos13", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.815], "Focus": [17741.3], "XYStage": [ 8109.200000000001, 32008.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos14", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.805], "Focus": [17741.3], "XYStage": [ 3483.3, 31908.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos15", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.82], "Focus": [17741.2], "XYStage": [ 2686.8, 31903.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos16", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [211.48000000000002], "Focus": [17741.1], "XYStage": [ -1978.9, 31973.600000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos17", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [211.51500000000001], "Focus": [17741.1], "XYStage": [ -2957.8, 31982.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos18", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.195], "Focus": [17741.5], "XYStage": [ 47747.4, -576.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos19", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.23000000000002], "Focus": [17741.6], "XYStage": [ 47053.9, -576.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos20", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.205], "Focus": [17741.4], "XYStage": [ 41871.200000000004, -479.8 ] }, "GridColumnIndex": 0 }, { "Label": "Pos21", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.4], "XYStage": [ 40882.600000000006, -392 ] }, "GridColumnIndex": 0 }, { "Label": "Pos22", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.24], "Focus": [17741.4], "XYStage": [ 35905.6, -135.3 ] }, "GridColumnIndex": 0 }, { "Label": "Pos23", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.195], "Focus": [17741.5], "XYStage": [ 34990.9, -132.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos24", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.46], "Focus": [17741.4], "XYStage": [ 29678.100000000002, -958.1 ] }, "GridColumnIndex": 0 }, { "Label": "Pos25", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.44], "Focus": [17741.5], "XYStage": [ 28983.600000000002, -939.4000000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos26", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.475], "Focus": [17741.5], "XYStage": [ 23019.7, -122 ] }, "GridColumnIndex": 0 }, { "Label": "Pos27", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.44], "Focus": [17741.7], "XYStage": [ 22428, -116.80000000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos28", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.46], "Focus": [17741.7], "XYStage": [ 16364.900000000001, -181.10000000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos29", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.48000000000002], "Focus": [17741.5], "XYStage": [ 15712.1, -180 ] }, "GridColumnIndex": 0 }, { "Label": "Pos30", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.45000000000002], "Focus": [17741.7], "XYStage": [ 10346.1, 77.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos31", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.49], "Focus": [17741.4], "XYStage": [ 9755.5, 85.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos32", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.475], "Focus": [17741.5], "XYStage": [ 4775.5, 293.90000000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos33", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.445], "Focus": [17741.4], "XYStage": [ 3954.9, 293.90000000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos34", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.455], "Focus": [17741.4], "XYStage": [ -1324.9, 545.6 ] }, "GridColumnIndex": 0 }, { "Label": "Pos35", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.445], "Focus": [17741.3], "XYStage": [ -2097, 576.6 ] }, "GridColumnIndex": 0 } ], "z-step_um": 10, "GridRow": 0, "BitDepth": 16, "Depth": 2, "Channels": 6, "Source": "Micro-Manager", "ChColors": [ -16711681, -256, -65281, -1, -1, -16776961 ], "MetadataVersion": 10, "KeepShutterOpenChannels": true, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536, 65536 ], "CustomIntervals_ms": [], "Height": 1200, "MicroManagerVersion": "1.4.23 20180219", "GridColumn": 0, "UUID": "67598d21-5db5-4842-ab60-f506f7b42c7c", "PixelType": "GRAY16", "Width": 1200, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\Yan\\20191218-YC113-S7S8-miseq", "Frames": 1, "Comment": "", "UserName": "Nikon", "PixelSize_um": 1.1, "ROI": [ 0, 0, 1200, 1200 ], "Date": "2019-12-18", "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC", "P-DAPI-high" ], "KeepShutterOpenSlices": true, "IJType": 1, "ComputerName": "ZadorConfocal2", "Positions": 36, "PositionIndex": 29, "CameraTimeout": 20000, "TimeFirst": false }, "640 AO-Name": "AnalogIO", "Vincent-D3-Command": "Close" }, "FrameKey-0-0-7": { "COM4-StopBits": "1", "528 AO-MaxVolts": "5.0000", "XLIGHT Excitation Wheel-State": "1", "470 Shutter-Name": "AnalogIO", "Core-Shutter": "520", "555 AO-Demo": "No", "XLIGHT Dichroic-State": "2", "XYStage-StepSize-Y": "0.1000", "ZPositionUm": 238.48000000000002, "XYStage-StepSize-X": "0.1000", "PRIME-FirmwareVersion": "20.28", "PRIME-ClearCycles": "2", "520-Name": "DA Shutter", "405 Shutter-Name": "AnalogIO", "PIEZO-StepSize": "0.0050", "PRIME-ClearMode": "Pre-Exposure", "470 AO-MinVolts": "0.0000", "405 AO-SupportsTriggering": "1", "CameraChannelIndex": 0, "XYStage-StartSpeed": "500.0000", "COM9-StopBits": "2", "405 AO-Description": "NI DAC", "COM4-DelayBetweenCharsMs": "0.0000", "445 AO-Block voltage": "false", "520 AO-TriggerInputLine": "/Dev1/PFI0", "555 AO-Description": "NI DAC", "520 AO-TriggerSampleRate": "100000", "COM4-Verbose": "1", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM4-Name": "COM4", "405 Shutter-Volts": "0.0000", "Vincent-D3-Port": "COM1", "PRIME-PP 4 MIN ADU AFFECTED": "200", "470 Shutter-MinVolts": "0.0000", "PRIME-PP 3 ENABLED": "Yes", "470 AO-Description": "NI DAC", "PRIME-Description": "PVCAM API device adapter", "520 Shutter-MaxVolts": "5.0000", "520 Shutter-Volts": "5.0000", "528 AO-Sequenceable": "1", "PRIME-Binning": "1x1", "555 Shutter-MaxVolts": "5.0000", "528 Shutter-TriggerSequenceLength": "1000", "405 Shutter-Block voltage": "false", "XLIGHT Spin motor-State": "1", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "Vincent-D3-Time to open (ms)": "35.0000", "Vincent-D3-Time to close (ms)": "35.0000", "445 AO-TriggerSampleRate": "100000", "640 Shutter-IOChannel": "Dev1/ao14", "555 AO-Sequenceable": "1", "520 AO-Name": "AnalogIO", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "470 AO-Block voltage": "false", "COM1-Verbose": "1", "COM9-AnswerTimeout": "10000.0000", "528 Shutter-Name": "AnalogIO", "PRIME-Trigger Timeout (secs)": "10", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "NextFrame": 0, "470 AO-TriggerSequenceLength": "1000", "PRIME-TransposeXY": "0", "445 AO-TriggerSequenceLength": "1000", "COM1-Name": "COM1", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "COM9-DataBits": "8", "COM1-AnswerTimeout": "500.0000", "Camera": "", "TE2000-Version": "V2.50-V2.50-V2.10", "528 Shutter-Volts": "0.0000", "XLIGHT Hub-Name": "XLIGHT Hub", "PIEZO-Description": "Ludl stage driver adapter", "PositionName": "Pos29", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSampleRate": "100000", "PRIME-FanSpeedSetpoint": "High", "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "405 AO-TriggerSampleRate": "100000", "470 Shutter-Block voltage": "false", "470-State": "0", "COM3-Parity": "None", "405 AO-Volts": "0.0000", "PRIME-TransposeMirrorY": "0", "PRIME-ChipName": "GS144BSI", "470 AO-Volts": "0.0000", "PRIME-TransposeMirrorX": "0", "445 Shutter-SupportsTriggering": "0", "470 Shutter-SupportsTriggering": "0", "XLIGHT Hub-Description": "XLIGHT hub", "PRIME-PreampOffLimit": "10000", "470 AO-Sequenceable": "1", "528 Shutter-MaxVolts": "5.0000", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "Core-XYStage": "XYStage", "470 AO-TriggerSampleRate": "100000", "PRIME-Timing-PreTriggerDelayNs": "24672000.0000", "528-DA Device": "528 Shutter", "Focus-Description": "Nikon TE2000 focus adapter", "FilterBlock-State": "5", "520 AO-Demo": "No", "COM3-DelayBetweenCharsMs": "0.0000", "470 Shutter-Volts": "0.0000", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "PRIME-PP 2 THRESHOLD": "125", "PRIME-PP 4 THRESHOLD": "75", "XYStage-Acceleration": "4.0000", "470 Shutter-Demo": "No", "PRIME-Name": "PMPCIECam00", "640-DA Device": "640 Shutter", "405 AO-Block voltage": "false", "ElapsedTime-ms": 1194296, "PRIME-Offset": "100", "445 AO-IOChannel": "Dev1/ao1", "640 AO-MaxVolts": "5.0000", "PositionIndex": 29, "PRIME-PP 1 ENABLED": "Yes", "COM1-BaudRate": "9600", "640-State": "0", "COM3-Verbose": "1", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "LudlController-TransmissionDelay": "0", "PRIME-FullWellCapacity": "62000", "PRIME-SMARTStreamingEnabled": "No", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "640 Shutter-Description": "NI DAC", "528-Description": "DA device that is used as a shutter", "COM4-Description": "Serial port driver (boost:asio)", "405 Shutter-Description": "NI DAC", "TE2000-Name": "TE2000", "528 Shutter-Description": "NI DAC", "COM9-Description": "Serial port driver (boost:asio)", "LudlController-Name": "LudlController", "445 Shutter-TriggerSequenceLength": "1000", "PRIME-SerialNumber": "A18B203008", "445 AO-Volts": "0.0000", "PRIME-X-dimension": "1200", "PRIME-ShutterCloseDelay": "0", "XLIGHT Disk slider-State": "2", "Position": "Default", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 AO-IOChannel": "Dev1/ao5", "520 AO-IOChannel": "Dev1/ao4", "528 AO-TriggerSequenceLength": "1000", "405 Shutter-MaxVolts": "5.0000", "470-DA Device": "470 Shutter", "PRIME-FTCapable": "No", "Core-ChannelGroup": "Channels", "555 AO-SupportsTriggering": "0", "PRIME-Y-dimension": "1200", "Frame": 0, "555 Shutter-Sequenceable": "1", "PRIME-Timing-ExposureTimeNs": "499998000.0000", "Core-ImageProcessor": "", "528-State": "0", "520 AO-Block voltage": "false", "XLIGHT Excitation Wheel-Label": "CFP/YFP", "640 Shutter-Demo": "No", "COM3-StopBits": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "520 Shutter-IOChannel": "Dev1/ao11", "Width": 1200, "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-SupportsTriggering": "0", "640 AO-TriggerSampleRate": "100000", "520-Description": "DA device that is used as a shutter", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "405 Shutter-Sequenceable": "1", "405 Shutter-SupportsTriggering": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-TriggerSampleRate": "100000", "640 Shutter-MaxVolts": "5.0000", "555 AO-MaxVolts": "5.0000", "520 AO-TriggerSequenceLength": "1000", "COM3-BaudRate": "9600", "COM9-BaudRate": "9600", "Vincent-D3-Channel #": "Ch. #1", "PRIME-PMode": "Normal", "OpticalPath-Label": "Left(100)", "BitDepth": 16, "555 AO-TriggerSequenceLength": "1000", "445 Shutter-Description": "NI DAC", "405 AO-Sequenceable": "1", "PRIME-AcquisitionMethod": "Callbacks", "555 AO-Name": "AnalogIO", "PRIME-ShutterMode": "Pre-Exposure", "COM3-Handshaking": "Off", "445 AO-SupportsTriggering": "0", "ROI": "0-0-1200-1200", "COM9-Verbose": "1", "PRIME-Color": "OFF", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "PRIME-ShutterOpenDelay": "0", "COM1-Parity": "None", "Slice": 7, "SlicePosition": 20, "COM9-DelayBetweenCharsMs": "0.0000", "555-Name": "DA Shutter", "PIEZO-LudlSingleAxisName": "B", "XLIGHT Emission Wheel-Label": "565/24(G)", "640 Shutter-Name": "AnalogIO", "640 AO-Sequenceable": "1", "520 Shutter-Demo": "No", "528 Shutter-Demo": "No", "470-Name": "DA Shutter", "520-DA Device": "520 Shutter", "XYStage-Description": "Ludl XY stage driver adapter", "445 Shutter-MinVolts": "0.0000", "528 AO-TriggerSampleRate": "100000", "405 AO-MinVolts": "0.0000", "405 Shutter-TriggerSequenceLength": "1000", "Height": 1200, "555 AO-Block voltage": "false", "640 Shutter-Volts": "0.0000", "PixelType": "GRAY16", "445 Shutter-Block voltage": "false", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "520 Shutter-TriggerSequenceLength": "1000", "COM9-Name": "COM9", "Core-SLM": "", "555 AO-Volts": "0.0000", "555 Shutter-Block voltage": "false", "XYStage-Speed": "1800.0000", "520 AO-MaxVolts": "5.0000", "528 Shutter-SupportsTriggering": "0", "COM1-Description": "Serial port driver (boost:asio)", "PRIME-MetadataEnabled": "No", "520 Shutter-MinVolts": "0.0000", "470 Shutter-Sequenceable": "1", "PRIME-PreampDelay": "0", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-MinVolts": "0.0000", "ChannelIndex": 0, "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Core-AutoShutter": "1", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "Exposure-ms": 320, "PRIME-PP 2 ENABLED": "Yes", "640 Shutter-TriggerSampleRate": "100000", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "470 Shutter-IOChannel": "Dev1/ao10", "405 AO-Demo": "No", "PixelSizeUm": 1.1, "470 AO-Demo": "No", "470 Shutter-MaxVolts": "5.0000", "Vincent-D3-Name": "Vincent-D3", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FileName": "img_000000000_Il-G_007.tif", "LudlController-Reset": "Operate", "PRIME-Exposure": "320.00", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "445-Name": "DA Shutter", "555 Shutter-Name": "AnalogIO", "520 Shutter-TriggerSampleRate": "100000", "445 Shutter-IOChannel": "Dev1/ao9", "PRIME-TransposeCorrection": "0", "XYStage-StepSize": "0.1000", "445-Description": "DA device that is used as a shutter", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 AO-IOChannel": "Dev1/ao7", "520 Shutter-Name": "AnalogIO", "COM3-DataBits": "8", "640 AO-Volts": "0.0000", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "555 Shutter-IOChannel": "Dev1/ao13", "WaitInterval": null, "520 Shutter-Sequenceable": "1", "YPositionUm": -180, "445-State": "0", "445-DA Device": "445 Shutter", "PRIME-CircularBufferAutoSize": "ON", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "555 AO-TriggerSampleRate": "100000", "445 Shutter-Volts": "0.0000", "XLIGHT Hub-Port": "COM3", "Core-AutoFocus": "", "COM4-Handshaking": "Off", "528 Shutter-MinVolts": "0.0000", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "445 Shutter-Name": "AnalogIO", "528 Shutter-Sequenceable": "1", "XYStage-TransposeMirrorX": "0", "XYStage-TransposeMirrorY": "0", "PRIME-ReadoutRate": "100MHz 16bit", "XYStage-Name": "Stage", "640 AO-SupportsTriggering": "0", "640-Name": "DA Shutter", "640 Shutter-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "640 AO-Block voltage": "false", "445 Shutter-Sequenceable": "1", "470 AO-MaxVolts": "5.0000", "COM4-BaudRate": "9600", "470-Description": "DA device that is used as a shutter", "520 Shutter-Description": "NI DAC", "PRIME-CCDTemperature": "-14.8500", "Time": "2019-12-18 23:10:54 -0500", "640 AO-TriggerSequenceLength": "1000", "470 AO-Name": "AnalogIO", "XLIGHT Disk slider-Label": "Disk Pos 40um", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "Binning": "1x1", "TE2000-ModelType": "1", "COM3-AnswerTimeout": "500.0000", "555 Shutter-SupportsTriggering": "0", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "TE2000-Port": "COM4", "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Description": "NI DAC", "COM4-AnswerTimeout": "500.0000", "COM1-DelayBetweenCharsMs": "0.0000", "FilterBlock-Label": "Confocal", "445 AO-Name": "AnalogIO", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-Timing-ReadoutTimeNs": "24672000.0000", "528 AO-Demo": "No", "520 AO-Sequenceable": "1", "555-Description": "DA device that is used as a shutter", "405-DA Device": "405 Shutter", "405 AO-TriggerSequenceLength": "1000", "640 AO-MinVolts": "0.0000", "640 Shutter-Block voltage": "false", "FrameIndex": 0, "PRIME-Port": "CMOS", "555 AO-MinVolts": "0.0000", "COM3-Description": "Serial port driver (boost:asio)", "528 AO-Description": "NI DAC", "555 AO-IOChannel": "Dev1/ao6", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "555 AO-TriggerInputLine": "/Dev1/PFI0", "PRIME-ActualInterval-ms": "390.5200", "LudlController-Port": "COM9", "445 Shutter-Demo": "No", "PRIME-Trigger-Expose Out-Mux": "1", "SliceIndex": 7, "PRIME-CCDTemperatureSetPoint": "-15.0000", "405 Shutter-MinVolts": "0.0000", "PRIME-ExposeOutMode": "First Row", "AxisPositions": null, "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Volts": "0.0000", "528-Name": "DA Shutter", "555-State": "0", "XLIGHT Dichroic-Label": "CFP/YFP", "445 AO-Sequenceable": "1", "405-Name": "DA Shutter", "405 AO-IOChannel": "Dev1/ao0", "Core-Camera": "PRIME", "Focus-Name": "Focus", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "405 Shutter-IOChannel": "Dev1/ao8", "640 AO-Demo": "No", "640 Shutter-SupportsTriggering": "0", "445 AO-Demo": "No", "555-DA Device": "555 Shutter", "470 Shutter-Description": "NI DAC", "528 AO-Volts": "0.0000", "COM1-DataBits": "8", "Vincent-D3-Address": "x", "520 AO-SupportsTriggering": "0", "405-Description": "DA device that is used as a shutter", "640 Shutter-MinVolts": "0.0000", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "405 AO-Name": "AnalogIO", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "640 AO-TriggerInputLine": "/Dev1/PFI0", "405 AO-TriggerInputLine": "/Dev1/PFI0", "640-Description": "DA device that is used as a shutter", "445 Shutter-TriggerSampleRate": "100000", "470 Shutter-TriggerSequenceLength": "1000", "COM1-StopBits": "1", "520 Shutter-SupportsTriggering": "0", "405-State": "0", "Core-Initialize": "1", "XLIGHT Emission Wheel-State": "3", "520 AO-Description": "NI DAC", "528 AO-TriggerInputLine": "/Dev1/PFI0", "445 Shutter-MaxVolts": "5.0000", "COM9-Handshaking": "Off", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "XLIGHT Spin motor-Label": "On (spinning)", "PRIME-PP 5 ENABLED": "No", "UUID": "2bd21e87-bd0b-4818-8498-6e7b5aa81853", "PRIME-TriggerMode": "Internal Trigger", "COM1-Handshaking": "Off", "PRIME-Timing-PostTriggerDelayNs": "49344000.0000", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "Focus-Position": "17741.5000", "528 Shutter-Block voltage": "false", "Channel": "Il-G", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "555 Shutter-Demo": "No", "405 Shutter-Demo": "No", "528 AO-Name": "AnalogIO", "520-State": "1", "Core-Galvo": "", "405 Shutter-TriggerSampleRate": "100000", "Focus-FocusStepSize_(nm)": "100", "PRIME-PixelType": "16bit", "COM4-DataBits": "8", "470 AO-IOChannel": "Dev1/ao2", "520 AO-MinVolts": "0.0000", "COM9-Parity": "None", "PRIME-CircularBufferEnabled": "ON", "PRIME-PP 6 Reset": "No", "640 AO-Description": "NI DAC", "Source": "PRIME", "445 AO-MinVolts": "0.0000", "PIEZO-Name": "Stage", "445 AO-Description": "NI DAC", "PRIME-CircularBufferFrameCount": "46", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "520 AO-Volts": "4.5000", "528 Shutter-TriggerSampleRate": "100000", "XPositionUm": 15712.1, "OpticalPath-State": "4", "PRIME-PP 3 THRESHOLD": "80", "PRIME-PVCAM Version": "3.7.1", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Gain": "1-HDR", "640 Shutter-TriggerSequenceLength": "1000", "528 AO-SupportsTriggering": "0", "445 AO-TriggerInputLine": "/Dev1/PFI0", "OpticalPath-Name": "OpticalPath", "PRIME-PP 4 ENABLED": "Yes", "PRIME-PVCAM Adapter Version": "1.3.29", "COM3-Name": "COM3", "405 AO-MaxVolts": "5.0000", "520 Shutter-Block voltage": "false", "PRIME-Timing-ClearingTimeNs": "49344000.0000", "Core-Focus": "PIEZO", "528 AO-Block voltage": "false", "TE2000-Description": "Nikon TE2000 hub", "528 AO-MinVolts": "0.0000", "COM4-Parity": "None", "Summary": { "Slices": 11, "ChContrastMin": [ 0, 0, 0, 0, 0, 0 ], "PixelAspect": 1, "SlicesFirst": true, "Interval_ms": 0, "Time": "2019-12-18 23:10:50 -0500", "Prefix": "seq_5", "InitialPositionList": [ { "Label": "Pos0", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.21], "Focus": [17741.5], "XYStage": [ 44542.200000000004, 31636.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos1", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.22], "Focus": [17741.4], "XYStage": [ 43801.100000000006, 31637.9 ] }, "GridColumnIndex": 0 }, { "Label": "Pos2", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.5], "XYStage": [ 38561.3, 32040.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos3", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.185], "Focus": [17741.3], "XYStage": [ 37698.4, 32041.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos4", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.16], "Focus": [17741.5], "XYStage": [ 32375.4, 31758.9 ] }, "GridColumnIndex": 0 }, { "Label": "Pos5", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.17000000000002], "Focus": [17741.3], "XYStage": [ 31770.7, 31758.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos6", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.4], "XYStage": [ 26536.800000000003, 31068.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos7", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.205], "Focus": [17741.4], "XYStage": [ 25420.2, 31068.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos8", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.85], "Focus": [17741.3], "XYStage": [ 21059, 31508 ] }, "GridColumnIndex": 0 }, { "Label": "Pos9", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.82], "Focus": [17741.3], "XYStage": [ 19731.9, 31506.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos10", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.79], "Focus": [17741.4], "XYStage": [ 14528.5, 32007.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos11", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.83], "Focus": [17741.2], "XYStage": [ 13830.300000000001, 32009.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos12", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.79], "Focus": [17741.3], "XYStage": [ 8775.300000000001, 32017.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos13", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.815], "Focus": [17741.3], "XYStage": [ 8109.200000000001, 32008.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos14", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.805], "Focus": [17741.3], "XYStage": [ 3483.3, 31908.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos15", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.82], "Focus": [17741.2], "XYStage": [ 2686.8, 31903.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos16", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [211.48000000000002], "Focus": [17741.1], "XYStage": [ -1978.9, 31973.600000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos17", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [211.51500000000001], "Focus": [17741.1], "XYStage": [ -2957.8, 31982.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos18", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.195], "Focus": [17741.5], "XYStage": [ 47747.4, -576.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos19", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.23000000000002], "Focus": [17741.6], "XYStage": [ 47053.9, -576.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos20", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.205], "Focus": [17741.4], "XYStage": [ 41871.200000000004, -479.8 ] }, "GridColumnIndex": 0 }, { "Label": "Pos21", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.4], "XYStage": [ 40882.600000000006, -392 ] }, "GridColumnIndex": 0 }, { "Label": "Pos22", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.24], "Focus": [17741.4], "XYStage": [ 35905.6, -135.3 ] }, "GridColumnIndex": 0 }, { "Label": "Pos23", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.195], "Focus": [17741.5], "XYStage": [ 34990.9, -132.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos24", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.46], "Focus": [17741.4], "XYStage": [ 29678.100000000002, -958.1 ] }, "GridColumnIndex": 0 }, { "Label": "Pos25", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.44], "Focus": [17741.5], "XYStage": [ 28983.600000000002, -939.4000000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos26", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.475], "Focus": [17741.5], "XYStage": [ 23019.7, -122 ] }, "GridColumnIndex": 0 }, { "Label": "Pos27", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.44], "Focus": [17741.7], "XYStage": [ 22428, -116.80000000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos28", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.46], "Focus": [17741.7], "XYStage": [ 16364.900000000001, -181.10000000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos29", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.48000000000002], "Focus": [17741.5], "XYStage": [ 15712.1, -180 ] }, "GridColumnIndex": 0 }, { "Label": "Pos30", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.45000000000002], "Focus": [17741.7], "XYStage": [ 10346.1, 77.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos31", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.49], "Focus": [17741.4], "XYStage": [ 9755.5, 85.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos32", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.475], "Focus": [17741.5], "XYStage": [ 4775.5, 293.90000000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos33", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.445], "Focus": [17741.4], "XYStage": [ 3954.9, 293.90000000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos34", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.455], "Focus": [17741.4], "XYStage": [ -1324.9, 545.6 ] }, "GridColumnIndex": 0 }, { "Label": "Pos35", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.445], "Focus": [17741.3], "XYStage": [ -2097, 576.6 ] }, "GridColumnIndex": 0 } ], "z-step_um": 10, "GridRow": 0, "BitDepth": 16, "Depth": 2, "Channels": 6, "Source": "Micro-Manager", "ChColors": [ -16711681, -256, -65281, -1, -1, -16776961 ], "MetadataVersion": 10, "KeepShutterOpenChannels": true, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536, 65536 ], "CustomIntervals_ms": [], "Height": 1200, "MicroManagerVersion": "1.4.23 20180219", "GridColumn": 0, "UUID": "67598d21-5db5-4842-ab60-f506f7b42c7c", "PixelType": "GRAY16", "Width": 1200, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\Yan\\20191218-YC113-S7S8-miseq", "Frames": 1, "Comment": "", "UserName": "Nikon", "PixelSize_um": 1.1, "ROI": [ 0, 0, 1200, 1200 ], "Date": "2019-12-18", "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC", "P-DAPI-high" ], "KeepShutterOpenSlices": true, "IJType": 1, "ComputerName": "ZadorConfocal2", "Positions": 36, "PositionIndex": 29, "CameraTimeout": 20000, "TimeFirst": false }, "640 AO-Name": "AnalogIO", "Vincent-D3-Command": "Close" }, "FrameKey-0-0-8": { "COM4-StopBits": "1", "528 AO-MaxVolts": "5.0000", "XLIGHT Excitation Wheel-State": "1", "470 Shutter-Name": "AnalogIO", "Core-Shutter": "520", "555 AO-Demo": "No", "XLIGHT Dichroic-State": "2", "XYStage-StepSize-Y": "0.1000", "ZPositionUm": 248.48000000000002, "XYStage-StepSize-X": "0.1000", "PRIME-FirmwareVersion": "20.28", "PRIME-ClearCycles": "2", "520-Name": "DA Shutter", "405 Shutter-Name": "AnalogIO", "PIEZO-StepSize": "0.0050", "PRIME-ClearMode": "Pre-Exposure", "470 AO-MinVolts": "0.0000", "405 AO-SupportsTriggering": "1", "CameraChannelIndex": 0, "XYStage-StartSpeed": "500.0000", "COM9-StopBits": "2", "405 AO-Description": "NI DAC", "COM4-DelayBetweenCharsMs": "0.0000", "445 AO-Block voltage": "false", "520 AO-TriggerInputLine": "/Dev1/PFI0", "555 AO-Description": "NI DAC", "520 AO-TriggerSampleRate": "100000", "COM4-Verbose": "1", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM4-Name": "COM4", "405 Shutter-Volts": "0.0000", "Vincent-D3-Port": "COM1", "PRIME-PP 4 MIN ADU AFFECTED": "200", "470 Shutter-MinVolts": "0.0000", "PRIME-PP 3 ENABLED": "Yes", "470 AO-Description": "NI DAC", "PRIME-Description": "PVCAM API device adapter", "520 Shutter-MaxVolts": "5.0000", "520 Shutter-Volts": "5.0000", "528 AO-Sequenceable": "1", "PRIME-Binning": "1x1", "555 Shutter-MaxVolts": "5.0000", "528 Shutter-TriggerSequenceLength": "1000", "405 Shutter-Block voltage": "false", "XLIGHT Spin motor-State": "1", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "Vincent-D3-Time to open (ms)": "35.0000", "Vincent-D3-Time to close (ms)": "35.0000", "445 AO-TriggerSampleRate": "100000", "640 Shutter-IOChannel": "Dev1/ao14", "555 AO-Sequenceable": "1", "520 AO-Name": "AnalogIO", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "470 AO-Block voltage": "false", "COM1-Verbose": "1", "COM9-AnswerTimeout": "10000.0000", "528 Shutter-Name": "AnalogIO", "PRIME-Trigger Timeout (secs)": "10", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "NextFrame": 0, "470 AO-TriggerSequenceLength": "1000", "PRIME-TransposeXY": "0", "445 AO-TriggerSequenceLength": "1000", "COM1-Name": "COM1", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "COM9-DataBits": "8", "COM1-AnswerTimeout": "500.0000", "Camera": "", "TE2000-Version": "V2.50-V2.50-V2.10", "528 Shutter-Volts": "0.0000", "XLIGHT Hub-Name": "XLIGHT Hub", "PIEZO-Description": "Ludl stage driver adapter", "PositionName": "Pos29", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSampleRate": "100000", "PRIME-FanSpeedSetpoint": "High", "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "405 AO-TriggerSampleRate": "100000", "470 Shutter-Block voltage": "false", "470-State": "0", "COM3-Parity": "None", "405 AO-Volts": "0.0000", "PRIME-TransposeMirrorY": "0", "PRIME-ChipName": "GS144BSI", "470 AO-Volts": "0.0000", "PRIME-TransposeMirrorX": "0", "445 Shutter-SupportsTriggering": "0", "470 Shutter-SupportsTriggering": "0", "XLIGHT Hub-Description": "XLIGHT hub", "PRIME-PreampOffLimit": "10000", "470 AO-Sequenceable": "1", "528 Shutter-MaxVolts": "5.0000", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "Core-XYStage": "XYStage", "470 AO-TriggerSampleRate": "100000", "PRIME-Timing-PreTriggerDelayNs": "24672000.0000", "528-DA Device": "528 Shutter", "Focus-Description": "Nikon TE2000 focus adapter", "FilterBlock-State": "5", "520 AO-Demo": "No", "COM3-DelayBetweenCharsMs": "0.0000", "470 Shutter-Volts": "0.0000", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "PRIME-PP 2 THRESHOLD": "125", "PRIME-PP 4 THRESHOLD": "75", "XYStage-Acceleration": "4.0000", "470 Shutter-Demo": "No", "PRIME-Name": "PMPCIECam00", "640-DA Device": "640 Shutter", "405 AO-Block voltage": "false", "ElapsedTime-ms": 1194863, "PRIME-Offset": "100", "445 AO-IOChannel": "Dev1/ao1", "640 AO-MaxVolts": "5.0000", "PositionIndex": 29, "PRIME-PP 1 ENABLED": "Yes", "COM1-BaudRate": "9600", "640-State": "0", "COM3-Verbose": "1", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "LudlController-TransmissionDelay": "0", "PRIME-FullWellCapacity": "62000", "PRIME-SMARTStreamingEnabled": "No", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "640 Shutter-Description": "NI DAC", "528-Description": "DA device that is used as a shutter", "COM4-Description": "Serial port driver (boost:asio)", "405 Shutter-Description": "NI DAC", "TE2000-Name": "TE2000", "528 Shutter-Description": "NI DAC", "COM9-Description": "Serial port driver (boost:asio)", "LudlController-Name": "LudlController", "445 Shutter-TriggerSequenceLength": "1000", "PRIME-SerialNumber": "A18B203008", "445 AO-Volts": "0.0000", "PRIME-X-dimension": "1200", "PRIME-ShutterCloseDelay": "0", "XLIGHT Disk slider-State": "2", "Position": "Default", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 AO-IOChannel": "Dev1/ao5", "520 AO-IOChannel": "Dev1/ao4", "528 AO-TriggerSequenceLength": "1000", "405 Shutter-MaxVolts": "5.0000", "470-DA Device": "470 Shutter", "PRIME-FTCapable": "No", "Core-ChannelGroup": "Channels", "555 AO-SupportsTriggering": "0", "PRIME-Y-dimension": "1200", "Frame": 0, "555 Shutter-Sequenceable": "1", "PRIME-Timing-ExposureTimeNs": "499998000.0000", "Core-ImageProcessor": "", "528-State": "0", "520 AO-Block voltage": "false", "XLIGHT Excitation Wheel-Label": "CFP/YFP", "640 Shutter-Demo": "No", "COM3-StopBits": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "520 Shutter-IOChannel": "Dev1/ao11", "Width": 1200, "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-SupportsTriggering": "0", "640 AO-TriggerSampleRate": "100000", "520-Description": "DA device that is used as a shutter", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "405 Shutter-Sequenceable": "1", "405 Shutter-SupportsTriggering": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-TriggerSampleRate": "100000", "640 Shutter-MaxVolts": "5.0000", "555 AO-MaxVolts": "5.0000", "520 AO-TriggerSequenceLength": "1000", "COM3-BaudRate": "9600", "COM9-BaudRate": "9600", "Vincent-D3-Channel #": "Ch. #1", "PRIME-PMode": "Normal", "OpticalPath-Label": "Left(100)", "BitDepth": 16, "555 AO-TriggerSequenceLength": "1000", "445 Shutter-Description": "NI DAC", "405 AO-Sequenceable": "1", "PRIME-AcquisitionMethod": "Callbacks", "555 AO-Name": "AnalogIO", "PRIME-ShutterMode": "Pre-Exposure", "COM3-Handshaking": "Off", "445 AO-SupportsTriggering": "0", "ROI": "0-0-1200-1200", "COM9-Verbose": "1", "PRIME-Color": "OFF", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "PRIME-ShutterOpenDelay": "0", "COM1-Parity": "None", "Slice": 8, "SlicePosition": 30, "COM9-DelayBetweenCharsMs": "0.0000", "555-Name": "DA Shutter", "PIEZO-LudlSingleAxisName": "B", "XLIGHT Emission Wheel-Label": "565/24(G)", "640 Shutter-Name": "AnalogIO", "640 AO-Sequenceable": "1", "520 Shutter-Demo": "No", "528 Shutter-Demo": "No", "470-Name": "DA Shutter", "520-DA Device": "520 Shutter", "XYStage-Description": "Ludl XY stage driver adapter", "445 Shutter-MinVolts": "0.0000", "528 AO-TriggerSampleRate": "100000", "405 AO-MinVolts": "0.0000", "405 Shutter-TriggerSequenceLength": "1000", "Height": 1200, "555 AO-Block voltage": "false", "640 Shutter-Volts": "0.0000", "PixelType": "GRAY16", "445 Shutter-Block voltage": "false", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "520 Shutter-TriggerSequenceLength": "1000", "COM9-Name": "COM9", "Core-SLM": "", "555 AO-Volts": "0.0000", "555 Shutter-Block voltage": "false", "XYStage-Speed": "1800.0000", "520 AO-MaxVolts": "5.0000", "528 Shutter-SupportsTriggering": "0", "COM1-Description": "Serial port driver (boost:asio)", "PRIME-MetadataEnabled": "No", "520 Shutter-MinVolts": "0.0000", "470 Shutter-Sequenceable": "1", "PRIME-PreampDelay": "0", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-MinVolts": "0.0000", "ChannelIndex": 0, "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Core-AutoShutter": "1", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "Exposure-ms": 320, "PRIME-PP 2 ENABLED": "Yes", "640 Shutter-TriggerSampleRate": "100000", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "470 Shutter-IOChannel": "Dev1/ao10", "405 AO-Demo": "No", "PixelSizeUm": 1.1, "470 AO-Demo": "No", "470 Shutter-MaxVolts": "5.0000", "Vincent-D3-Name": "Vincent-D3", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FileName": "img_000000000_Il-G_008.tif", "LudlController-Reset": "Operate", "PRIME-Exposure": "320.00", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "445-Name": "DA Shutter", "555 Shutter-Name": "AnalogIO", "520 Shutter-TriggerSampleRate": "100000", "445 Shutter-IOChannel": "Dev1/ao9", "PRIME-TransposeCorrection": "0", "XYStage-StepSize": "0.1000", "445-Description": "DA device that is used as a shutter", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 AO-IOChannel": "Dev1/ao7", "520 Shutter-Name": "AnalogIO", "COM3-DataBits": "8", "640 AO-Volts": "0.0000", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "555 Shutter-IOChannel": "Dev1/ao13", "WaitInterval": null, "520 Shutter-Sequenceable": "1", "YPositionUm": -180, "445-State": "0", "445-DA Device": "445 Shutter", "PRIME-CircularBufferAutoSize": "ON", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "555 AO-TriggerSampleRate": "100000", "445 Shutter-Volts": "0.0000", "XLIGHT Hub-Port": "COM3", "Core-AutoFocus": "", "COM4-Handshaking": "Off", "528 Shutter-MinVolts": "0.0000", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "445 Shutter-Name": "AnalogIO", "528 Shutter-Sequenceable": "1", "XYStage-TransposeMirrorX": "0", "XYStage-TransposeMirrorY": "0", "PRIME-ReadoutRate": "100MHz 16bit", "XYStage-Name": "Stage", "640 AO-SupportsTriggering": "0", "640-Name": "DA Shutter", "640 Shutter-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "640 AO-Block voltage": "false", "445 Shutter-Sequenceable": "1", "470 AO-MaxVolts": "5.0000", "COM4-BaudRate": "9600", "470-Description": "DA device that is used as a shutter", "520 Shutter-Description": "NI DAC", "PRIME-CCDTemperature": "-14.8500", "Time": "2019-12-18 23:10:55 -0500", "640 AO-TriggerSequenceLength": "1000", "470 AO-Name": "AnalogIO", "XLIGHT Disk slider-Label": "Disk Pos 40um", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "Binning": "1x1", "TE2000-ModelType": "1", "COM3-AnswerTimeout": "500.0000", "555 Shutter-SupportsTriggering": "0", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "TE2000-Port": "COM4", "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Description": "NI DAC", "COM4-AnswerTimeout": "500.0000", "COM1-DelayBetweenCharsMs": "0.0000", "FilterBlock-Label": "Confocal", "445 AO-Name": "AnalogIO", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-Timing-ReadoutTimeNs": "24672000.0000", "528 AO-Demo": "No", "520 AO-Sequenceable": "1", "555-Description": "DA device that is used as a shutter", "405-DA Device": "405 Shutter", "405 AO-TriggerSequenceLength": "1000", "640 AO-MinVolts": "0.0000", "640 Shutter-Block voltage": "false", "FrameIndex": 0, "PRIME-Port": "CMOS", "555 AO-MinVolts": "0.0000", "COM3-Description": "Serial port driver (boost:asio)", "528 AO-Description": "NI DAC", "555 AO-IOChannel": "Dev1/ao6", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "555 AO-TriggerInputLine": "/Dev1/PFI0", "PRIME-ActualInterval-ms": "390.5200", "LudlController-Port": "COM9", "445 Shutter-Demo": "No", "PRIME-Trigger-Expose Out-Mux": "1", "SliceIndex": 8, "PRIME-CCDTemperatureSetPoint": "-15.0000", "405 Shutter-MinVolts": "0.0000", "PRIME-ExposeOutMode": "First Row", "AxisPositions": null, "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Volts": "0.0000", "528-Name": "DA Shutter", "555-State": "0", "XLIGHT Dichroic-Label": "CFP/YFP", "445 AO-Sequenceable": "1", "405-Name": "DA Shutter", "405 AO-IOChannel": "Dev1/ao0", "Core-Camera": "PRIME", "Focus-Name": "Focus", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "405 Shutter-IOChannel": "Dev1/ao8", "640 AO-Demo": "No", "640 Shutter-SupportsTriggering": "0", "445 AO-Demo": "No", "555-DA Device": "555 Shutter", "470 Shutter-Description": "NI DAC", "528 AO-Volts": "0.0000", "COM1-DataBits": "8", "Vincent-D3-Address": "x", "520 AO-SupportsTriggering": "0", "405-Description": "DA device that is used as a shutter", "640 Shutter-MinVolts": "0.0000", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "405 AO-Name": "AnalogIO", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "640 AO-TriggerInputLine": "/Dev1/PFI0", "405 AO-TriggerInputLine": "/Dev1/PFI0", "640-Description": "DA device that is used as a shutter", "445 Shutter-TriggerSampleRate": "100000", "470 Shutter-TriggerSequenceLength": "1000", "COM1-StopBits": "1", "520 Shutter-SupportsTriggering": "0", "405-State": "0", "Core-Initialize": "1", "XLIGHT Emission Wheel-State": "3", "520 AO-Description": "NI DAC", "528 AO-TriggerInputLine": "/Dev1/PFI0", "445 Shutter-MaxVolts": "5.0000", "COM9-Handshaking": "Off", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "XLIGHT Spin motor-Label": "On (spinning)", "PRIME-PP 5 ENABLED": "No", "UUID": "77a04481-03cf-4ec2-b622-e730260abe51", "PRIME-TriggerMode": "Internal Trigger", "COM1-Handshaking": "Off", "PRIME-Timing-PostTriggerDelayNs": "49344000.0000", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "Focus-Position": "17741.5000", "528 Shutter-Block voltage": "false", "Channel": "Il-G", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "555 Shutter-Demo": "No", "405 Shutter-Demo": "No", "528 AO-Name": "AnalogIO", "520-State": "1", "Core-Galvo": "", "405 Shutter-TriggerSampleRate": "100000", "Focus-FocusStepSize_(nm)": "100", "PRIME-PixelType": "16bit", "COM4-DataBits": "8", "470 AO-IOChannel": "Dev1/ao2", "520 AO-MinVolts": "0.0000", "COM9-Parity": "None", "PRIME-CircularBufferEnabled": "ON", "PRIME-PP 6 Reset": "No", "640 AO-Description": "NI DAC", "Source": "PRIME", "445 AO-MinVolts": "0.0000", "PIEZO-Name": "Stage", "445 AO-Description": "NI DAC", "PRIME-CircularBufferFrameCount": "46", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "520 AO-Volts": "4.5000", "528 Shutter-TriggerSampleRate": "100000", "XPositionUm": 15712.1, "OpticalPath-State": "4", "PRIME-PP 3 THRESHOLD": "80", "PRIME-PVCAM Version": "3.7.1", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Gain": "1-HDR", "640 Shutter-TriggerSequenceLength": "1000", "528 AO-SupportsTriggering": "0", "445 AO-TriggerInputLine": "/Dev1/PFI0", "OpticalPath-Name": "OpticalPath", "PRIME-PP 4 ENABLED": "Yes", "PRIME-PVCAM Adapter Version": "1.3.29", "COM3-Name": "COM3", "405 AO-MaxVolts": "5.0000", "520 Shutter-Block voltage": "false", "PRIME-Timing-ClearingTimeNs": "49344000.0000", "Core-Focus": "PIEZO", "528 AO-Block voltage": "false", "TE2000-Description": "Nikon TE2000 hub", "528 AO-MinVolts": "0.0000", "COM4-Parity": "None", "Summary": { "Slices": 11, "ChContrastMin": [ 0, 0, 0, 0, 0, 0 ], "PixelAspect": 1, "SlicesFirst": true, "Interval_ms": 0, "Time": "2019-12-18 23:10:50 -0500", "Prefix": "seq_5", "InitialPositionList": [ { "Label": "Pos0", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.21], "Focus": [17741.5], "XYStage": [ 44542.200000000004, 31636.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos1", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.22], "Focus": [17741.4], "XYStage": [ 43801.100000000006, 31637.9 ] }, "GridColumnIndex": 0 }, { "Label": "Pos2", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.5], "XYStage": [ 38561.3, 32040.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos3", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.185], "Focus": [17741.3], "XYStage": [ 37698.4, 32041.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos4", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.16], "Focus": [17741.5], "XYStage": [ 32375.4, 31758.9 ] }, "GridColumnIndex": 0 }, { "Label": "Pos5", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.17000000000002], "Focus": [17741.3], "XYStage": [ 31770.7, 31758.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos6", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.4], "XYStage": [ 26536.800000000003, 31068.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos7", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.205], "Focus": [17741.4], "XYStage": [ 25420.2, 31068.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos8", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.85], "Focus": [17741.3], "XYStage": [ 21059, 31508 ] }, "GridColumnIndex": 0 }, { "Label": "Pos9", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.82], "Focus": [17741.3], "XYStage": [ 19731.9, 31506.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos10", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.79], "Focus": [17741.4], "XYStage": [ 14528.5, 32007.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos11", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.83], "Focus": [17741.2], "XYStage": [ 13830.300000000001, 32009.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos12", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.79], "Focus": [17741.3], "XYStage": [ 8775.300000000001, 32017.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos13", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.815], "Focus": [17741.3], "XYStage": [ 8109.200000000001, 32008.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos14", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.805], "Focus": [17741.3], "XYStage": [ 3483.3, 31908.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos15", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.82], "Focus": [17741.2], "XYStage": [ 2686.8, 31903.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos16", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [211.48000000000002], "Focus": [17741.1], "XYStage": [ -1978.9, 31973.600000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos17", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [211.51500000000001], "Focus": [17741.1], "XYStage": [ -2957.8, 31982.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos18", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.195], "Focus": [17741.5], "XYStage": [ 47747.4, -576.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos19", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.23000000000002], "Focus": [17741.6], "XYStage": [ 47053.9, -576.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos20", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.205], "Focus": [17741.4], "XYStage": [ 41871.200000000004, -479.8 ] }, "GridColumnIndex": 0 }, { "Label": "Pos21", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.4], "XYStage": [ 40882.600000000006, -392 ] }, "GridColumnIndex": 0 }, { "Label": "Pos22", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.24], "Focus": [17741.4], "XYStage": [ 35905.6, -135.3 ] }, "GridColumnIndex": 0 }, { "Label": "Pos23", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.195], "Focus": [17741.5], "XYStage": [ 34990.9, -132.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos24", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.46], "Focus": [17741.4], "XYStage": [ 29678.100000000002, -958.1 ] }, "GridColumnIndex": 0 }, { "Label": "Pos25", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.44], "Focus": [17741.5], "XYStage": [ 28983.600000000002, -939.4000000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos26", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.475], "Focus": [17741.5], "XYStage": [ 23019.7, -122 ] }, "GridColumnIndex": 0 }, { "Label": "Pos27", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.44], "Focus": [17741.7], "XYStage": [ 22428, -116.80000000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos28", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.46], "Focus": [17741.7], "XYStage": [ 16364.900000000001, -181.10000000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos29", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.48000000000002], "Focus": [17741.5], "XYStage": [ 15712.1, -180 ] }, "GridColumnIndex": 0 }, { "Label": "Pos30", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.45000000000002], "Focus": [17741.7], "XYStage": [ 10346.1, 77.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos31", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.49], "Focus": [17741.4], "XYStage": [ 9755.5, 85.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos32", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.475], "Focus": [17741.5], "XYStage": [ 4775.5, 293.90000000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos33", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.445], "Focus": [17741.4], "XYStage": [ 3954.9, 293.90000000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos34", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.455], "Focus": [17741.4], "XYStage": [ -1324.9, 545.6 ] }, "GridColumnIndex": 0 }, { "Label": "Pos35", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.445], "Focus": [17741.3], "XYStage": [ -2097, 576.6 ] }, "GridColumnIndex": 0 } ], "z-step_um": 10, "GridRow": 0, "BitDepth": 16, "Depth": 2, "Channels": 6, "Source": "Micro-Manager", "ChColors": [ -16711681, -256, -65281, -1, -1, -16776961 ], "MetadataVersion": 10, "KeepShutterOpenChannels": true, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536, 65536 ], "CustomIntervals_ms": [], "Height": 1200, "MicroManagerVersion": "1.4.23 20180219", "GridColumn": 0, "UUID": "67598d21-5db5-4842-ab60-f506f7b42c7c", "PixelType": "GRAY16", "Width": 1200, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\Yan\\20191218-YC113-S7S8-miseq", "Frames": 1, "Comment": "", "UserName": "Nikon", "PixelSize_um": 1.1, "ROI": [ 0, 0, 1200, 1200 ], "Date": "2019-12-18", "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC", "P-DAPI-high" ], "KeepShutterOpenSlices": true, "IJType": 1, "ComputerName": "ZadorConfocal2", "Positions": 36, "PositionIndex": 29, "CameraTimeout": 20000, "TimeFirst": false }, "640 AO-Name": "AnalogIO", "Vincent-D3-Command": "Close" }, "FrameKey-0-0-9": { "COM4-StopBits": "1", "528 AO-MaxVolts": "5.0000", "XLIGHT Excitation Wheel-State": "1", "470 Shutter-Name": "AnalogIO", "Core-Shutter": "520", "555 AO-Demo": "No", "XLIGHT Dichroic-State": "2", "XYStage-StepSize-Y": "0.1000", "ZPositionUm": 258.48, "XYStage-StepSize-X": "0.1000", "PRIME-FirmwareVersion": "20.28", "PRIME-ClearCycles": "2", "520-Name": "DA Shutter", "405 Shutter-Name": "AnalogIO", "PIEZO-StepSize": "0.0050", "PRIME-ClearMode": "Pre-Exposure", "470 AO-MinVolts": "0.0000", "405 AO-SupportsTriggering": "1", "CameraChannelIndex": 0, "XYStage-StartSpeed": "500.0000", "COM9-StopBits": "2", "405 AO-Description": "NI DAC", "COM4-DelayBetweenCharsMs": "0.0000", "445 AO-Block voltage": "false", "520 AO-TriggerInputLine": "/Dev1/PFI0", "555 AO-Description": "NI DAC", "520 AO-TriggerSampleRate": "100000", "COM4-Verbose": "1", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM4-Name": "COM4", "405 Shutter-Volts": "0.0000", "Vincent-D3-Port": "COM1", "PRIME-PP 4 MIN ADU AFFECTED": "200", "470 Shutter-MinVolts": "0.0000", "PRIME-PP 3 ENABLED": "Yes", "470 AO-Description": "NI DAC", "PRIME-Description": "PVCAM API device adapter", "520 Shutter-MaxVolts": "5.0000", "520 Shutter-Volts": "5.0000", "528 AO-Sequenceable": "1", "PRIME-Binning": "1x1", "555 Shutter-MaxVolts": "5.0000", "528 Shutter-TriggerSequenceLength": "1000", "405 Shutter-Block voltage": "false", "XLIGHT Spin motor-State": "1", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "Vincent-D3-Time to open (ms)": "35.0000", "Vincent-D3-Time to close (ms)": "35.0000", "445 AO-TriggerSampleRate": "100000", "640 Shutter-IOChannel": "Dev1/ao14", "555 AO-Sequenceable": "1", "520 AO-Name": "AnalogIO", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "470 AO-Block voltage": "false", "COM1-Verbose": "1", "COM9-AnswerTimeout": "10000.0000", "528 Shutter-Name": "AnalogIO", "PRIME-Trigger Timeout (secs)": "10", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "NextFrame": 0, "470 AO-TriggerSequenceLength": "1000", "PRIME-TransposeXY": "0", "445 AO-TriggerSequenceLength": "1000", "COM1-Name": "COM1", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "COM9-DataBits": "8", "COM1-AnswerTimeout": "500.0000", "Camera": "", "TE2000-Version": "V2.50-V2.50-V2.10", "528 Shutter-Volts": "0.0000", "XLIGHT Hub-Name": "XLIGHT Hub", "PIEZO-Description": "Ludl stage driver adapter", "PositionName": "Pos29", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSampleRate": "100000", "PRIME-FanSpeedSetpoint": "High", "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "405 AO-TriggerSampleRate": "100000", "470 Shutter-Block voltage": "false", "470-State": "0", "COM3-Parity": "None", "405 AO-Volts": "0.0000", "PRIME-TransposeMirrorY": "0", "PRIME-ChipName": "GS144BSI", "470 AO-Volts": "0.0000", "PRIME-TransposeMirrorX": "0", "445 Shutter-SupportsTriggering": "0", "470 Shutter-SupportsTriggering": "0", "XLIGHT Hub-Description": "XLIGHT hub", "PRIME-PreampOffLimit": "10000", "470 AO-Sequenceable": "1", "528 Shutter-MaxVolts": "5.0000", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "Core-XYStage": "XYStage", "470 AO-TriggerSampleRate": "100000", "PRIME-Timing-PreTriggerDelayNs": "24672000.0000", "528-DA Device": "528 Shutter", "Focus-Description": "Nikon TE2000 focus adapter", "FilterBlock-State": "5", "520 AO-Demo": "No", "COM3-DelayBetweenCharsMs": "0.0000", "470 Shutter-Volts": "0.0000", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "PRIME-PP 2 THRESHOLD": "125", "PRIME-PP 4 THRESHOLD": "75", "XYStage-Acceleration": "4.0000", "470 Shutter-Demo": "No", "PRIME-Name": "PMPCIECam00", "640-DA Device": "640 Shutter", "405 AO-Block voltage": "false", "ElapsedTime-ms": 1195414, "PRIME-Offset": "100", "445 AO-IOChannel": "Dev1/ao1", "640 AO-MaxVolts": "5.0000", "PositionIndex": 29, "PRIME-PP 1 ENABLED": "Yes", "COM1-BaudRate": "9600", "640-State": "0", "COM3-Verbose": "1", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "LudlController-TransmissionDelay": "0", "PRIME-FullWellCapacity": "62000", "PRIME-SMARTStreamingEnabled": "No", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "640 Shutter-Description": "NI DAC", "528-Description": "DA device that is used as a shutter", "COM4-Description": "Serial port driver (boost:asio)", "405 Shutter-Description": "NI DAC", "TE2000-Name": "TE2000", "528 Shutter-Description": "NI DAC", "COM9-Description": "Serial port driver (boost:asio)", "LudlController-Name": "LudlController", "445 Shutter-TriggerSequenceLength": "1000", "PRIME-SerialNumber": "A18B203008", "445 AO-Volts": "0.0000", "PRIME-X-dimension": "1200", "PRIME-ShutterCloseDelay": "0", "XLIGHT Disk slider-State": "2", "Position": "Default", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 AO-IOChannel": "Dev1/ao5", "520 AO-IOChannel": "Dev1/ao4", "528 AO-TriggerSequenceLength": "1000", "405 Shutter-MaxVolts": "5.0000", "470-DA Device": "470 Shutter", "PRIME-FTCapable": "No", "Core-ChannelGroup": "Channels", "555 AO-SupportsTriggering": "0", "PRIME-Y-dimension": "1200", "Frame": 0, "555 Shutter-Sequenceable": "1", "PRIME-Timing-ExposureTimeNs": "499998000.0000", "Core-ImageProcessor": "", "528-State": "0", "520 AO-Block voltage": "false", "XLIGHT Excitation Wheel-Label": "CFP/YFP", "640 Shutter-Demo": "No", "COM3-StopBits": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "520 Shutter-IOChannel": "Dev1/ao11", "Width": 1200, "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-SupportsTriggering": "0", "640 AO-TriggerSampleRate": "100000", "520-Description": "DA device that is used as a shutter", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "405 Shutter-Sequenceable": "1", "405 Shutter-SupportsTriggering": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-TriggerSampleRate": "100000", "640 Shutter-MaxVolts": "5.0000", "555 AO-MaxVolts": "5.0000", "520 AO-TriggerSequenceLength": "1000", "COM3-BaudRate": "9600", "COM9-BaudRate": "9600", "Vincent-D3-Channel #": "Ch. #1", "PRIME-PMode": "Normal", "OpticalPath-Label": "Left(100)", "BitDepth": 16, "555 AO-TriggerSequenceLength": "1000", "445 Shutter-Description": "NI DAC", "405 AO-Sequenceable": "1", "PRIME-AcquisitionMethod": "Callbacks", "555 AO-Name": "AnalogIO", "PRIME-ShutterMode": "Pre-Exposure", "COM3-Handshaking": "Off", "445 AO-SupportsTriggering": "0", "ROI": "0-0-1200-1200", "COM9-Verbose": "1", "PRIME-Color": "OFF", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "PRIME-ShutterOpenDelay": "0", "COM1-Parity": "None", "Slice": 9, "SlicePosition": 40, "COM9-DelayBetweenCharsMs": "0.0000", "555-Name": "DA Shutter", "PIEZO-LudlSingleAxisName": "B", "XLIGHT Emission Wheel-Label": "565/24(G)", "640 Shutter-Name": "AnalogIO", "640 AO-Sequenceable": "1", "520 Shutter-Demo": "No", "528 Shutter-Demo": "No", "470-Name": "DA Shutter", "520-DA Device": "520 Shutter", "XYStage-Description": "Ludl XY stage driver adapter", "445 Shutter-MinVolts": "0.0000", "528 AO-TriggerSampleRate": "100000", "405 AO-MinVolts": "0.0000", "405 Shutter-TriggerSequenceLength": "1000", "Height": 1200, "555 AO-Block voltage": "false", "640 Shutter-Volts": "0.0000", "PixelType": "GRAY16", "445 Shutter-Block voltage": "false", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "520 Shutter-TriggerSequenceLength": "1000", "COM9-Name": "COM9", "Core-SLM": "", "555 AO-Volts": "0.0000", "555 Shutter-Block voltage": "false", "XYStage-Speed": "1800.0000", "520 AO-MaxVolts": "5.0000", "528 Shutter-SupportsTriggering": "0", "COM1-Description": "Serial port driver (boost:asio)", "PRIME-MetadataEnabled": "No", "520 Shutter-MinVolts": "0.0000", "470 Shutter-Sequenceable": "1", "PRIME-PreampDelay": "0", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-MinVolts": "0.0000", "ChannelIndex": 0, "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Core-AutoShutter": "1", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "Exposure-ms": 320, "PRIME-PP 2 ENABLED": "Yes", "640 Shutter-TriggerSampleRate": "100000", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "470 Shutter-IOChannel": "Dev1/ao10", "405 AO-Demo": "No", "PixelSizeUm": 1.1, "470 AO-Demo": "No", "470 Shutter-MaxVolts": "5.0000", "Vincent-D3-Name": "Vincent-D3", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FileName": "img_000000000_Il-G_009.tif", "LudlController-Reset": "Operate", "PRIME-Exposure": "320.00", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "445-Name": "DA Shutter", "555 Shutter-Name": "AnalogIO", "520 Shutter-TriggerSampleRate": "100000", "445 Shutter-IOChannel": "Dev1/ao9", "PRIME-TransposeCorrection": "0", "XYStage-StepSize": "0.1000", "445-Description": "DA device that is used as a shutter", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 AO-IOChannel": "Dev1/ao7", "520 Shutter-Name": "AnalogIO", "COM3-DataBits": "8", "640 AO-Volts": "0.0000", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "555 Shutter-IOChannel": "Dev1/ao13", "WaitInterval": null, "520 Shutter-Sequenceable": "1", "YPositionUm": -180, "445-State": "0", "445-DA Device": "445 Shutter", "PRIME-CircularBufferAutoSize": "ON", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "555 AO-TriggerSampleRate": "100000", "445 Shutter-Volts": "0.0000", "XLIGHT Hub-Port": "COM3", "Core-AutoFocus": "", "COM4-Handshaking": "Off", "528 Shutter-MinVolts": "0.0000", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "445 Shutter-Name": "AnalogIO", "528 Shutter-Sequenceable": "1", "XYStage-TransposeMirrorX": "0", "XYStage-TransposeMirrorY": "0", "PRIME-ReadoutRate": "100MHz 16bit", "XYStage-Name": "Stage", "640 AO-SupportsTriggering": "0", "640-Name": "DA Shutter", "640 Shutter-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "640 AO-Block voltage": "false", "445 Shutter-Sequenceable": "1", "470 AO-MaxVolts": "5.0000", "COM4-BaudRate": "9600", "470-Description": "DA device that is used as a shutter", "520 Shutter-Description": "NI DAC", "PRIME-CCDTemperature": "-14.8500", "Time": "2019-12-18 23:10:55 -0500", "640 AO-TriggerSequenceLength": "1000", "470 AO-Name": "AnalogIO", "XLIGHT Disk slider-Label": "Disk Pos 40um", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "Binning": "1x1", "TE2000-ModelType": "1", "COM3-AnswerTimeout": "500.0000", "555 Shutter-SupportsTriggering": "0", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "TE2000-Port": "COM4", "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Description": "NI DAC", "COM4-AnswerTimeout": "500.0000", "COM1-DelayBetweenCharsMs": "0.0000", "FilterBlock-Label": "Confocal", "445 AO-Name": "AnalogIO", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-Timing-ReadoutTimeNs": "24672000.0000", "528 AO-Demo": "No", "520 AO-Sequenceable": "1", "555-Description": "DA device that is used as a shutter", "405-DA Device": "405 Shutter", "405 AO-TriggerSequenceLength": "1000", "640 AO-MinVolts": "0.0000", "640 Shutter-Block voltage": "false", "FrameIndex": 0, "PRIME-Port": "CMOS", "555 AO-MinVolts": "0.0000", "COM3-Description": "Serial port driver (boost:asio)", "528 AO-Description": "NI DAC", "555 AO-IOChannel": "Dev1/ao6", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "555 AO-TriggerInputLine": "/Dev1/PFI0", "PRIME-ActualInterval-ms": "390.5200", "LudlController-Port": "COM9", "445 Shutter-Demo": "No", "PRIME-Trigger-Expose Out-Mux": "1", "SliceIndex": 9, "PRIME-CCDTemperatureSetPoint": "-15.0000", "405 Shutter-MinVolts": "0.0000", "PRIME-ExposeOutMode": "First Row", "AxisPositions": null, "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Volts": "0.0000", "528-Name": "DA Shutter", "555-State": "0", "XLIGHT Dichroic-Label": "CFP/YFP", "445 AO-Sequenceable": "1", "405-Name": "DA Shutter", "405 AO-IOChannel": "Dev1/ao0", "Core-Camera": "PRIME", "Focus-Name": "Focus", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "405 Shutter-IOChannel": "Dev1/ao8", "640 AO-Demo": "No", "640 Shutter-SupportsTriggering": "0", "445 AO-Demo": "No", "555-DA Device": "555 Shutter", "470 Shutter-Description": "NI DAC", "528 AO-Volts": "0.0000", "COM1-DataBits": "8", "Vincent-D3-Address": "x", "520 AO-SupportsTriggering": "0", "405-Description": "DA device that is used as a shutter", "640 Shutter-MinVolts": "0.0000", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "405 AO-Name": "AnalogIO", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "640 AO-TriggerInputLine": "/Dev1/PFI0", "405 AO-TriggerInputLine": "/Dev1/PFI0", "640-Description": "DA device that is used as a shutter", "445 Shutter-TriggerSampleRate": "100000", "470 Shutter-TriggerSequenceLength": "1000", "COM1-StopBits": "1", "520 Shutter-SupportsTriggering": "0", "405-State": "0", "Core-Initialize": "1", "XLIGHT Emission Wheel-State": "3", "520 AO-Description": "NI DAC", "528 AO-TriggerInputLine": "/Dev1/PFI0", "445 Shutter-MaxVolts": "5.0000", "COM9-Handshaking": "Off", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "XLIGHT Spin motor-Label": "On (spinning)", "PRIME-PP 5 ENABLED": "No", "UUID": "c8109474-0d64-4155-9521-1df645ee0065", "PRIME-TriggerMode": "Internal Trigger", "COM1-Handshaking": "Off", "PRIME-Timing-PostTriggerDelayNs": "49344000.0000", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "Focus-Position": "17741.5000", "528 Shutter-Block voltage": "false", "Channel": "Il-G", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "555 Shutter-Demo": "No", "405 Shutter-Demo": "No", "528 AO-Name": "AnalogIO", "520-State": "1", "Core-Galvo": "", "405 Shutter-TriggerSampleRate": "100000", "Focus-FocusStepSize_(nm)": "100", "PRIME-PixelType": "16bit", "COM4-DataBits": "8", "470 AO-IOChannel": "Dev1/ao2", "520 AO-MinVolts": "0.0000", "COM9-Parity": "None", "PRIME-CircularBufferEnabled": "ON", "PRIME-PP 6 Reset": "No", "640 AO-Description": "NI DAC", "Source": "PRIME", "445 AO-MinVolts": "0.0000", "PIEZO-Name": "Stage", "445 AO-Description": "NI DAC", "PRIME-CircularBufferFrameCount": "46", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "520 AO-Volts": "4.5000", "528 Shutter-TriggerSampleRate": "100000", "XPositionUm": 15712.1, "OpticalPath-State": "4", "PRIME-PP 3 THRESHOLD": "80", "PRIME-PVCAM Version": "3.7.1", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Gain": "1-HDR", "640 Shutter-TriggerSequenceLength": "1000", "528 AO-SupportsTriggering": "0", "445 AO-TriggerInputLine": "/Dev1/PFI0", "OpticalPath-Name": "OpticalPath", "PRIME-PP 4 ENABLED": "Yes", "PRIME-PVCAM Adapter Version": "1.3.29", "COM3-Name": "COM3", "405 AO-MaxVolts": "5.0000", "520 Shutter-Block voltage": "false", "PRIME-Timing-ClearingTimeNs": "49344000.0000", "Core-Focus": "PIEZO", "528 AO-Block voltage": "false", "TE2000-Description": "Nikon TE2000 hub", "528 AO-MinVolts": "0.0000", "COM4-Parity": "None", "Summary": { "Slices": 11, "ChContrastMin": [ 0, 0, 0, 0, 0, 0 ], "PixelAspect": 1, "SlicesFirst": true, "Interval_ms": 0, "Time": "2019-12-18 23:10:50 -0500", "Prefix": "seq_5", "InitialPositionList": [ { "Label": "Pos0", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.21], "Focus": [17741.5], "XYStage": [ 44542.200000000004, 31636.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos1", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.22], "Focus": [17741.4], "XYStage": [ 43801.100000000006, 31637.9 ] }, "GridColumnIndex": 0 }, { "Label": "Pos2", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.5], "XYStage": [ 38561.3, 32040.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos3", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.185], "Focus": [17741.3], "XYStage": [ 37698.4, 32041.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos4", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.16], "Focus": [17741.5], "XYStage": [ 32375.4, 31758.9 ] }, "GridColumnIndex": 0 }, { "Label": "Pos5", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.17000000000002], "Focus": [17741.3], "XYStage": [ 31770.7, 31758.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos6", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.4], "XYStage": [ 26536.800000000003, 31068.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos7", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.205], "Focus": [17741.4], "XYStage": [ 25420.2, 31068.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos8", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.85], "Focus": [17741.3], "XYStage": [ 21059, 31508 ] }, "GridColumnIndex": 0 }, { "Label": "Pos9", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.82], "Focus": [17741.3], "XYStage": [ 19731.9, 31506.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos10", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.79], "Focus": [17741.4], "XYStage": [ 14528.5, 32007.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos11", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.83], "Focus": [17741.2], "XYStage": [ 13830.300000000001, 32009.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos12", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.79], "Focus": [17741.3], "XYStage": [ 8775.300000000001, 32017.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos13", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.815], "Focus": [17741.3], "XYStage": [ 8109.200000000001, 32008.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos14", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.805], "Focus": [17741.3], "XYStage": [ 3483.3, 31908.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos15", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.82], "Focus": [17741.2], "XYStage": [ 2686.8, 31903.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos16", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [211.48000000000002], "Focus": [17741.1], "XYStage": [ -1978.9, 31973.600000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos17", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [211.51500000000001], "Focus": [17741.1], "XYStage": [ -2957.8, 31982.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos18", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.195], "Focus": [17741.5], "XYStage": [ 47747.4, -576.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos19", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.23000000000002], "Focus": [17741.6], "XYStage": [ 47053.9, -576.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos20", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.205], "Focus": [17741.4], "XYStage": [ 41871.200000000004, -479.8 ] }, "GridColumnIndex": 0 }, { "Label": "Pos21", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.4], "XYStage": [ 40882.600000000006, -392 ] }, "GridColumnIndex": 0 }, { "Label": "Pos22", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.24], "Focus": [17741.4], "XYStage": [ 35905.6, -135.3 ] }, "GridColumnIndex": 0 }, { "Label": "Pos23", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.195], "Focus": [17741.5], "XYStage": [ 34990.9, -132.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos24", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.46], "Focus": [17741.4], "XYStage": [ 29678.100000000002, -958.1 ] }, "GridColumnIndex": 0 }, { "Label": "Pos25", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.44], "Focus": [17741.5], "XYStage": [ 28983.600000000002, -939.4000000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos26", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.475], "Focus": [17741.5], "XYStage": [ 23019.7, -122 ] }, "GridColumnIndex": 0 }, { "Label": "Pos27", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.44], "Focus": [17741.7], "XYStage": [ 22428, -116.80000000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos28", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.46], "Focus": [17741.7], "XYStage": [ 16364.900000000001, -181.10000000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos29", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.48000000000002], "Focus": [17741.5], "XYStage": [ 15712.1, -180 ] }, "GridColumnIndex": 0 }, { "Label": "Pos30", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.45000000000002], "Focus": [17741.7], "XYStage": [ 10346.1, 77.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos31", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.49], "Focus": [17741.4], "XYStage": [ 9755.5, 85.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos32", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.475], "Focus": [17741.5], "XYStage": [ 4775.5, 293.90000000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos33", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.445], "Focus": [17741.4], "XYStage": [ 3954.9, 293.90000000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos34", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.455], "Focus": [17741.4], "XYStage": [ -1324.9, 545.6 ] }, "GridColumnIndex": 0 }, { "Label": "Pos35", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.445], "Focus": [17741.3], "XYStage": [ -2097, 576.6 ] }, "GridColumnIndex": 0 } ], "z-step_um": 10, "GridRow": 0, "BitDepth": 16, "Depth": 2, "Channels": 6, "Source": "Micro-Manager", "ChColors": [ -16711681, -256, -65281, -1, -1, -16776961 ], "MetadataVersion": 10, "KeepShutterOpenChannels": true, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536, 65536 ], "CustomIntervals_ms": [], "Height": 1200, "MicroManagerVersion": "1.4.23 20180219", "GridColumn": 0, "UUID": "67598d21-5db5-4842-ab60-f506f7b42c7c", "PixelType": "GRAY16", "Width": 1200, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\Yan\\20191218-YC113-S7S8-miseq", "Frames": 1, "Comment": "", "UserName": "Nikon", "PixelSize_um": 1.1, "ROI": [ 0, 0, 1200, 1200 ], "Date": "2019-12-18", "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC", "P-DAPI-high" ], "KeepShutterOpenSlices": true, "IJType": 1, "ComputerName": "ZadorConfocal2", "Positions": 36, "PositionIndex": 29, "CameraTimeout": 20000, "TimeFirst": false }, "640 AO-Name": "AnalogIO", "Vincent-D3-Command": "Close" }, "FrameKey-0-0-10": { "COM4-StopBits": "1", "528 AO-MaxVolts": "5.0000", "XLIGHT Excitation Wheel-State": "1", "470 Shutter-Name": "AnalogIO", "Core-Shutter": "520", "555 AO-Demo": "No", "XLIGHT Dichroic-State": "2", "XYStage-StepSize-Y": "0.1000", "ZPositionUm": 268.48, "XYStage-StepSize-X": "0.1000", "PRIME-FirmwareVersion": "20.28", "PRIME-ClearCycles": "2", "520-Name": "DA Shutter", "405 Shutter-Name": "AnalogIO", "PIEZO-StepSize": "0.0050", "PRIME-ClearMode": "Pre-Exposure", "470 AO-MinVolts": "0.0000", "405 AO-SupportsTriggering": "1", "CameraChannelIndex": 0, "XYStage-StartSpeed": "500.0000", "COM9-StopBits": "2", "405 AO-Description": "NI DAC", "COM4-DelayBetweenCharsMs": "0.0000", "445 AO-Block voltage": "false", "520 AO-TriggerInputLine": "/Dev1/PFI0", "555 AO-Description": "NI DAC", "520 AO-TriggerSampleRate": "100000", "COM4-Verbose": "1", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM4-Name": "COM4", "405 Shutter-Volts": "0.0000", "Vincent-D3-Port": "COM1", "PRIME-PP 4 MIN ADU AFFECTED": "200", "470 Shutter-MinVolts": "0.0000", "PRIME-PP 3 ENABLED": "Yes", "470 AO-Description": "NI DAC", "PRIME-Description": "PVCAM API device adapter", "520 Shutter-MaxVolts": "5.0000", "520 Shutter-Volts": "5.0000", "528 AO-Sequenceable": "1", "PRIME-Binning": "1x1", "555 Shutter-MaxVolts": "5.0000", "528 Shutter-TriggerSequenceLength": "1000", "405 Shutter-Block voltage": "false", "XLIGHT Spin motor-State": "1", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "Vincent-D3-Time to open (ms)": "35.0000", "Vincent-D3-Time to close (ms)": "35.0000", "445 AO-TriggerSampleRate": "100000", "640 Shutter-IOChannel": "Dev1/ao14", "555 AO-Sequenceable": "1", "520 AO-Name": "AnalogIO", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "470 AO-Block voltage": "false", "COM1-Verbose": "1", "COM9-AnswerTimeout": "10000.0000", "528 Shutter-Name": "AnalogIO", "PRIME-Trigger Timeout (secs)": "10", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "NextFrame": 0, "470 AO-TriggerSequenceLength": "1000", "PRIME-TransposeXY": "0", "445 AO-TriggerSequenceLength": "1000", "COM1-Name": "COM1", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "COM9-DataBits": "8", "COM1-AnswerTimeout": "500.0000", "Camera": "", "TE2000-Version": "V2.50-V2.50-V2.10", "528 Shutter-Volts": "0.0000", "XLIGHT Hub-Name": "XLIGHT Hub", "PIEZO-Description": "Ludl stage driver adapter", "PositionName": "Pos29", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSampleRate": "100000", "PRIME-FanSpeedSetpoint": "High", "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "405 AO-TriggerSampleRate": "100000", "470 Shutter-Block voltage": "false", "470-State": "0", "COM3-Parity": "None", "405 AO-Volts": "0.0000", "PRIME-TransposeMirrorY": "0", "PRIME-ChipName": "GS144BSI", "470 AO-Volts": "0.0000", "PRIME-TransposeMirrorX": "0", "445 Shutter-SupportsTriggering": "0", "470 Shutter-SupportsTriggering": "0", "XLIGHT Hub-Description": "XLIGHT hub", "PRIME-PreampOffLimit": "10000", "470 AO-Sequenceable": "1", "528 Shutter-MaxVolts": "5.0000", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "Core-XYStage": "XYStage", "470 AO-TriggerSampleRate": "100000", "PRIME-Timing-PreTriggerDelayNs": "24672000.0000", "528-DA Device": "528 Shutter", "Focus-Description": "Nikon TE2000 focus adapter", "FilterBlock-State": "5", "520 AO-Demo": "No", "COM3-DelayBetweenCharsMs": "0.0000", "470 Shutter-Volts": "0.0000", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "PRIME-PP 2 THRESHOLD": "125", "PRIME-PP 4 THRESHOLD": "75", "XYStage-Acceleration": "4.0000", "470 Shutter-Demo": "No", "PRIME-Name": "PMPCIECam00", "640-DA Device": "640 Shutter", "405 AO-Block voltage": "false", "ElapsedTime-ms": 1196029, "PRIME-Offset": "100", "445 AO-IOChannel": "Dev1/ao1", "640 AO-MaxVolts": "5.0000", "PositionIndex": 29, "PRIME-PP 1 ENABLED": "Yes", "COM1-BaudRate": "9600", "640-State": "0", "COM3-Verbose": "1", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "LudlController-TransmissionDelay": "0", "PRIME-FullWellCapacity": "62000", "PRIME-SMARTStreamingEnabled": "No", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "640 Shutter-Description": "NI DAC", "528-Description": "DA device that is used as a shutter", "COM4-Description": "Serial port driver (boost:asio)", "405 Shutter-Description": "NI DAC", "TE2000-Name": "TE2000", "528 Shutter-Description": "NI DAC", "COM9-Description": "Serial port driver (boost:asio)", "LudlController-Name": "LudlController", "445 Shutter-TriggerSequenceLength": "1000", "PRIME-SerialNumber": "A18B203008", "445 AO-Volts": "0.0000", "PRIME-X-dimension": "1200", "PRIME-ShutterCloseDelay": "0", "XLIGHT Disk slider-State": "2", "Position": "Default", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 AO-IOChannel": "Dev1/ao5", "520 AO-IOChannel": "Dev1/ao4", "528 AO-TriggerSequenceLength": "1000", "405 Shutter-MaxVolts": "5.0000", "470-DA Device": "470 Shutter", "PRIME-FTCapable": "No", "Core-ChannelGroup": "Channels", "555 AO-SupportsTriggering": "0", "PRIME-Y-dimension": "1200", "Frame": 0, "555 Shutter-Sequenceable": "1", "PRIME-Timing-ExposureTimeNs": "499998000.0000", "Core-ImageProcessor": "", "528-State": "0", "520 AO-Block voltage": "false", "XLIGHT Excitation Wheel-Label": "CFP/YFP", "640 Shutter-Demo": "No", "COM3-StopBits": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "520 Shutter-IOChannel": "Dev1/ao11", "Width": 1200, "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-SupportsTriggering": "0", "640 AO-TriggerSampleRate": "100000", "520-Description": "DA device that is used as a shutter", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "405 Shutter-Sequenceable": "1", "405 Shutter-SupportsTriggering": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-TriggerSampleRate": "100000", "640 Shutter-MaxVolts": "5.0000", "555 AO-MaxVolts": "5.0000", "520 AO-TriggerSequenceLength": "1000", "COM3-BaudRate": "9600", "COM9-BaudRate": "9600", "Vincent-D3-Channel #": "Ch. #1", "PRIME-PMode": "Normal", "OpticalPath-Label": "Left(100)", "BitDepth": 16, "555 AO-TriggerSequenceLength": "1000", "445 Shutter-Description": "NI DAC", "405 AO-Sequenceable": "1", "PRIME-AcquisitionMethod": "Callbacks", "555 AO-Name": "AnalogIO", "PRIME-ShutterMode": "Pre-Exposure", "COM3-Handshaking": "Off", "445 AO-SupportsTriggering": "0", "ROI": "0-0-1200-1200", "COM9-Verbose": "1", "PRIME-Color": "OFF", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "PRIME-ShutterOpenDelay": "0", "COM1-Parity": "None", "Slice": 10, "SlicePosition": 50, "COM9-DelayBetweenCharsMs": "0.0000", "555-Name": "DA Shutter", "PIEZO-LudlSingleAxisName": "B", "XLIGHT Emission Wheel-Label": "565/24(G)", "640 Shutter-Name": "AnalogIO", "640 AO-Sequenceable": "1", "520 Shutter-Demo": "No", "528 Shutter-Demo": "No", "470-Name": "DA Shutter", "520-DA Device": "520 Shutter", "XYStage-Description": "Ludl XY stage driver adapter", "445 Shutter-MinVolts": "0.0000", "528 AO-TriggerSampleRate": "100000", "405 AO-MinVolts": "0.0000", "405 Shutter-TriggerSequenceLength": "1000", "Height": 1200, "555 AO-Block voltage": "false", "640 Shutter-Volts": "0.0000", "PixelType": "GRAY16", "445 Shutter-Block voltage": "false", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "520 Shutter-TriggerSequenceLength": "1000", "COM9-Name": "COM9", "Core-SLM": "", "555 AO-Volts": "0.0000", "555 Shutter-Block voltage": "false", "XYStage-Speed": "1800.0000", "520 AO-MaxVolts": "5.0000", "528 Shutter-SupportsTriggering": "0", "COM1-Description": "Serial port driver (boost:asio)", "PRIME-MetadataEnabled": "No", "520 Shutter-MinVolts": "0.0000", "470 Shutter-Sequenceable": "1", "PRIME-PreampDelay": "0", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-MinVolts": "0.0000", "ChannelIndex": 0, "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Core-AutoShutter": "1", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "Exposure-ms": 320, "PRIME-PP 2 ENABLED": "Yes", "640 Shutter-TriggerSampleRate": "100000", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "470 Shutter-IOChannel": "Dev1/ao10", "405 AO-Demo": "No", "PixelSizeUm": 1.1, "470 AO-Demo": "No", "470 Shutter-MaxVolts": "5.0000", "Vincent-D3-Name": "Vincent-D3", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FileName": "img_000000000_Il-G_010.tif", "LudlController-Reset": "Operate", "PRIME-Exposure": "320.00", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "445-Name": "DA Shutter", "555 Shutter-Name": "AnalogIO", "520 Shutter-TriggerSampleRate": "100000", "445 Shutter-IOChannel": "Dev1/ao9", "PRIME-TransposeCorrection": "0", "XYStage-StepSize": "0.1000", "445-Description": "DA device that is used as a shutter", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 AO-IOChannel": "Dev1/ao7", "520 Shutter-Name": "AnalogIO", "COM3-DataBits": "8", "640 AO-Volts": "0.0000", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "555 Shutter-IOChannel": "Dev1/ao13", "WaitInterval": null, "520 Shutter-Sequenceable": "1", "YPositionUm": -180, "445-State": "0", "445-DA Device": "445 Shutter", "PRIME-CircularBufferAutoSize": "ON", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "555 AO-TriggerSampleRate": "100000", "445 Shutter-Volts": "0.0000", "XLIGHT Hub-Port": "COM3", "Core-AutoFocus": "", "COM4-Handshaking": "Off", "528 Shutter-MinVolts": "0.0000", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "445 Shutter-Name": "AnalogIO", "528 Shutter-Sequenceable": "1", "XYStage-TransposeMirrorX": "0", "XYStage-TransposeMirrorY": "0", "PRIME-ReadoutRate": "100MHz 16bit", "XYStage-Name": "Stage", "640 AO-SupportsTriggering": "0", "640-Name": "DA Shutter", "640 Shutter-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "640 AO-Block voltage": "false", "445 Shutter-Sequenceable": "1", "470 AO-MaxVolts": "5.0000", "COM4-BaudRate": "9600", "470-Description": "DA device that is used as a shutter", "520 Shutter-Description": "NI DAC", "PRIME-CCDTemperature": "-14.8500", "Time": "2019-12-18 23:10:56 -0500", "640 AO-TriggerSequenceLength": "1000", "470 AO-Name": "AnalogIO", "XLIGHT Disk slider-Label": "Disk Pos 40um", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "Binning": "1x1", "TE2000-ModelType": "1", "COM3-AnswerTimeout": "500.0000", "555 Shutter-SupportsTriggering": "0", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "TE2000-Port": "COM4", "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Description": "NI DAC", "COM4-AnswerTimeout": "500.0000", "COM1-DelayBetweenCharsMs": "0.0000", "FilterBlock-Label": "Confocal", "445 AO-Name": "AnalogIO", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-Timing-ReadoutTimeNs": "24672000.0000", "528 AO-Demo": "No", "520 AO-Sequenceable": "1", "555-Description": "DA device that is used as a shutter", "405-DA Device": "405 Shutter", "405 AO-TriggerSequenceLength": "1000", "640 AO-MinVolts": "0.0000", "640 Shutter-Block voltage": "false", "FrameIndex": 0, "PRIME-Port": "CMOS", "555 AO-MinVolts": "0.0000", "COM3-Description": "Serial port driver (boost:asio)", "528 AO-Description": "NI DAC", "555 AO-IOChannel": "Dev1/ao6", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "555 AO-TriggerInputLine": "/Dev1/PFI0", "PRIME-ActualInterval-ms": "390.5200", "LudlController-Port": "COM9", "445 Shutter-Demo": "No", "PRIME-Trigger-Expose Out-Mux": "1", "SliceIndex": 10, "PRIME-CCDTemperatureSetPoint": "-15.0000", "405 Shutter-MinVolts": "0.0000", "PRIME-ExposeOutMode": "First Row", "AxisPositions": null, "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Volts": "0.0000", "528-Name": "DA Shutter", "555-State": "0", "XLIGHT Dichroic-Label": "CFP/YFP", "445 AO-Sequenceable": "1", "405-Name": "DA Shutter", "405 AO-IOChannel": "Dev1/ao0", "Core-Camera": "PRIME", "Focus-Name": "Focus", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "405 Shutter-IOChannel": "Dev1/ao8", "640 AO-Demo": "No", "640 Shutter-SupportsTriggering": "0", "445 AO-Demo": "No", "555-DA Device": "555 Shutter", "470 Shutter-Description": "NI DAC", "528 AO-Volts": "0.0000", "COM1-DataBits": "8", "Vincent-D3-Address": "x", "520 AO-SupportsTriggering": "0", "405-Description": "DA device that is used as a shutter", "640 Shutter-MinVolts": "0.0000", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "405 AO-Name": "AnalogIO", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "640 AO-TriggerInputLine": "/Dev1/PFI0", "405 AO-TriggerInputLine": "/Dev1/PFI0", "640-Description": "DA device that is used as a shutter", "445 Shutter-TriggerSampleRate": "100000", "470 Shutter-TriggerSequenceLength": "1000", "COM1-StopBits": "1", "520 Shutter-SupportsTriggering": "0", "405-State": "0", "Core-Initialize": "1", "XLIGHT Emission Wheel-State": "3", "520 AO-Description": "NI DAC", "528 AO-TriggerInputLine": "/Dev1/PFI0", "445 Shutter-MaxVolts": "5.0000", "COM9-Handshaking": "Off", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "XLIGHT Spin motor-Label": "On (spinning)", "PRIME-PP 5 ENABLED": "No", "UUID": "0183a052-8286-48a0-8596-19c50b2f6764", "PRIME-TriggerMode": "Internal Trigger", "COM1-Handshaking": "Off", "PRIME-Timing-PostTriggerDelayNs": "49344000.0000", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "Focus-Position": "17741.5000", "528 Shutter-Block voltage": "false", "Channel": "Il-G", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "555 Shutter-Demo": "No", "405 Shutter-Demo": "No", "528 AO-Name": "AnalogIO", "520-State": "0", "Core-Galvo": "", "405 Shutter-TriggerSampleRate": "100000", "Focus-FocusStepSize_(nm)": "100", "PRIME-PixelType": "16bit", "COM4-DataBits": "8", "470 AO-IOChannel": "Dev1/ao2", "520 AO-MinVolts": "0.0000", "COM9-Parity": "None", "PRIME-CircularBufferEnabled": "ON", "PRIME-PP 6 Reset": "No", "640 AO-Description": "NI DAC", "Source": "PRIME", "445 AO-MinVolts": "0.0000", "PIEZO-Name": "Stage", "445 AO-Description": "NI DAC", "PRIME-CircularBufferFrameCount": "46", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "520 AO-Volts": "4.5000", "528 Shutter-TriggerSampleRate": "100000", "XPositionUm": 15712.1, "OpticalPath-State": "4", "PRIME-PP 3 THRESHOLD": "80", "PRIME-PVCAM Version": "3.7.1", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Gain": "1-HDR", "640 Shutter-TriggerSequenceLength": "1000", "528 AO-SupportsTriggering": "0", "445 AO-TriggerInputLine": "/Dev1/PFI0", "OpticalPath-Name": "OpticalPath", "PRIME-PP 4 ENABLED": "Yes", "PRIME-PVCAM Adapter Version": "1.3.29", "COM3-Name": "COM3", "405 AO-MaxVolts": "5.0000", "520 Shutter-Block voltage": "false", "PRIME-Timing-ClearingTimeNs": "49344000.0000", "Core-Focus": "PIEZO", "528 AO-Block voltage": "false", "TE2000-Description": "Nikon TE2000 hub", "528 AO-MinVolts": "0.0000", "COM4-Parity": "None", "Summary": { "Slices": 11, "ChContrastMin": [ 0, 0, 0, 0, 0, 0 ], "PixelAspect": 1, "SlicesFirst": true, "Interval_ms": 0, "Time": "2019-12-18 23:10:50 -0500", "Prefix": "seq_5", "InitialPositionList": [ { "Label": "Pos0", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.21], "Focus": [17741.5], "XYStage": [ 44542.200000000004, 31636.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos1", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.22], "Focus": [17741.4], "XYStage": [ 43801.100000000006, 31637.9 ] }, "GridColumnIndex": 0 }, { "Label": "Pos2", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.5], "XYStage": [ 38561.3, 32040.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos3", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.185], "Focus": [17741.3], "XYStage": [ 37698.4, 32041.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos4", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.16], "Focus": [17741.5], "XYStage": [ 32375.4, 31758.9 ] }, "GridColumnIndex": 0 }, { "Label": "Pos5", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.17000000000002], "Focus": [17741.3], "XYStage": [ 31770.7, 31758.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos6", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.4], "XYStage": [ 26536.800000000003, 31068.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos7", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.205], "Focus": [17741.4], "XYStage": [ 25420.2, 31068.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos8", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.85], "Focus": [17741.3], "XYStage": [ 21059, 31508 ] }, "GridColumnIndex": 0 }, { "Label": "Pos9", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.82], "Focus": [17741.3], "XYStage": [ 19731.9, 31506.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos10", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.79], "Focus": [17741.4], "XYStage": [ 14528.5, 32007.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos11", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.83], "Focus": [17741.2], "XYStage": [ 13830.300000000001, 32009.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos12", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.79], "Focus": [17741.3], "XYStage": [ 8775.300000000001, 32017.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos13", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.815], "Focus": [17741.3], "XYStage": [ 8109.200000000001, 32008.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos14", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.805], "Focus": [17741.3], "XYStage": [ 3483.3, 31908.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos15", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.82], "Focus": [17741.2], "XYStage": [ 2686.8, 31903.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos16", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [211.48000000000002], "Focus": [17741.1], "XYStage": [ -1978.9, 31973.600000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos17", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [211.51500000000001], "Focus": [17741.1], "XYStage": [ -2957.8, 31982.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos18", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.195], "Focus": [17741.5], "XYStage": [ 47747.4, -576.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos19", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.23000000000002], "Focus": [17741.6], "XYStage": [ 47053.9, -576.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos20", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.205], "Focus": [17741.4], "XYStage": [ 41871.200000000004, -479.8 ] }, "GridColumnIndex": 0 }, { "Label": "Pos21", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.4], "XYStage": [ 40882.600000000006, -392 ] }, "GridColumnIndex": 0 }, { "Label": "Pos22", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.24], "Focus": [17741.4], "XYStage": [ 35905.6, -135.3 ] }, "GridColumnIndex": 0 }, { "Label": "Pos23", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.195], "Focus": [17741.5], "XYStage": [ 34990.9, -132.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos24", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.46], "Focus": [17741.4], "XYStage": [ 29678.100000000002, -958.1 ] }, "GridColumnIndex": 0 }, { "Label": "Pos25", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.44], "Focus": [17741.5], "XYStage": [ 28983.600000000002, -939.4000000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos26", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.475], "Focus": [17741.5], "XYStage": [ 23019.7, -122 ] }, "GridColumnIndex": 0 }, { "Label": "Pos27", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.44], "Focus": [17741.7], "XYStage": [ 22428, -116.80000000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos28", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.46], "Focus": [17741.7], "XYStage": [ 16364.900000000001, -181.10000000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos29", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.48000000000002], "Focus": [17741.5], "XYStage": [ 15712.1, -180 ] }, "GridColumnIndex": 0 }, { "Label": "Pos30", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.45000000000002], "Focus": [17741.7], "XYStage": [ 10346.1, 77.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos31", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.49], "Focus": [17741.4], "XYStage": [ 9755.5, 85.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos32", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.475], "Focus": [17741.5], "XYStage": [ 4775.5, 293.90000000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos33", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.445], "Focus": [17741.4], "XYStage": [ 3954.9, 293.90000000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos34", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.455], "Focus": [17741.4], "XYStage": [ -1324.9, 545.6 ] }, "GridColumnIndex": 0 }, { "Label": "Pos35", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.445], "Focus": [17741.3], "XYStage": [ -2097, 576.6 ] }, "GridColumnIndex": 0 } ], "z-step_um": 10, "GridRow": 0, "BitDepth": 16, "Depth": 2, "Channels": 6, "Source": "Micro-Manager", "ChColors": [ -16711681, -256, -65281, -1, -1, -16776961 ], "MetadataVersion": 10, "KeepShutterOpenChannels": true, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536, 65536 ], "CustomIntervals_ms": [], "Height": 1200, "MicroManagerVersion": "1.4.23 20180219", "GridColumn": 0, "UUID": "67598d21-5db5-4842-ab60-f506f7b42c7c", "PixelType": "GRAY16", "Width": 1200, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\Yan\\20191218-YC113-S7S8-miseq", "Frames": 1, "Comment": "", "UserName": "Nikon", "PixelSize_um": 1.1, "ROI": [ 0, 0, 1200, 1200 ], "Date": "2019-12-18", "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC", "P-DAPI-high" ], "KeepShutterOpenSlices": true, "IJType": 1, "ComputerName": "ZadorConfocal2", "Positions": 36, "PositionIndex": 29, "CameraTimeout": 20000, "TimeFirst": false }, "640 AO-Name": "AnalogIO", "Vincent-D3-Command": "Close" }, "FrameKey-0-1-0": { "COM4-StopBits": "1", "528 AO-MaxVolts": "5.0000", "XLIGHT Excitation Wheel-State": "1", "470 Shutter-Name": "AnalogIO", "Core-Shutter": "555", "555 AO-Demo": "No", "XLIGHT Dichroic-State": "2", "XYStage-StepSize-Y": "0.1000", "ZPositionUm": 168.48000000000002, "XYStage-StepSize-X": "0.1000", "PRIME-FirmwareVersion": "20.28", "PRIME-ClearCycles": "2", "520-Name": "DA Shutter", "405 Shutter-Name": "AnalogIO", "PIEZO-StepSize": "0.0050", "PRIME-ClearMode": "Pre-Exposure", "470 AO-MinVolts": "0.0000", "405 AO-SupportsTriggering": "1", "CameraChannelIndex": 0, "XYStage-StartSpeed": "500.0000", "COM9-StopBits": "2", "405 AO-Description": "NI DAC", "COM4-DelayBetweenCharsMs": "0.0000", "445 AO-Block voltage": "false", "520 AO-TriggerInputLine": "/Dev1/PFI0", "555 AO-Description": "NI DAC", "520 AO-TriggerSampleRate": "100000", "COM4-Verbose": "1", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM4-Name": "COM4", "405 Shutter-Volts": "0.0000", "Vincent-D3-Port": "COM1", "PRIME-PP 4 MIN ADU AFFECTED": "200", "470 Shutter-MinVolts": "0.0000", "PRIME-PP 3 ENABLED": "Yes", "470 AO-Description": "NI DAC", "PRIME-Description": "PVCAM API device adapter", "520 Shutter-MaxVolts": "5.0000", "520 Shutter-Volts": "0.0000", "528 AO-Sequenceable": "1", "PRIME-Binning": "1x1", "555 Shutter-MaxVolts": "5.0000", "528 Shutter-TriggerSequenceLength": "1000", "405 Shutter-Block voltage": "false", "XLIGHT Spin motor-State": "1", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "Vincent-D3-Time to open (ms)": "35.0000", "Vincent-D3-Time to close (ms)": "35.0000", "445 AO-TriggerSampleRate": "100000", "640 Shutter-IOChannel": "Dev1/ao14", "555 AO-Sequenceable": "1", "520 AO-Name": "AnalogIO", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "470 AO-Block voltage": "false", "COM1-Verbose": "1", "COM9-AnswerTimeout": "10000.0000", "528 Shutter-Name": "AnalogIO", "PRIME-Trigger Timeout (secs)": "10", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "NextFrame": 0, "470 AO-TriggerSequenceLength": "1000", "PRIME-TransposeXY": "0", "445 AO-TriggerSequenceLength": "1000", "COM1-Name": "COM1", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "COM9-DataBits": "8", "COM1-AnswerTimeout": "500.0000", "Camera": "", "TE2000-Version": "V2.50-V2.50-V2.10", "528 Shutter-Volts": "0.0000", "XLIGHT Hub-Name": "XLIGHT Hub", "PIEZO-Description": "Ludl stage driver adapter", "PositionName": "Pos29", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSampleRate": "100000", "PRIME-FanSpeedSetpoint": "High", "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "405 AO-TriggerSampleRate": "100000", "470 Shutter-Block voltage": "false", "470-State": "0", "COM3-Parity": "None", "405 AO-Volts": "0.0000", "PRIME-TransposeMirrorY": "0", "PRIME-ChipName": "GS144BSI", "470 AO-Volts": "0.0000", "PRIME-TransposeMirrorX": "0", "445 Shutter-SupportsTriggering": "0", "470 Shutter-SupportsTriggering": "0", "XLIGHT Hub-Description": "XLIGHT hub", "PRIME-PreampOffLimit": "10000", "470 AO-Sequenceable": "1", "528 Shutter-MaxVolts": "5.0000", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "Core-XYStage": "XYStage", "470 AO-TriggerSampleRate": "100000", "PRIME-Timing-PreTriggerDelayNs": "24672000.0000", "528-DA Device": "528 Shutter", "Focus-Description": "Nikon TE2000 focus adapter", "FilterBlock-State": "5", "520 AO-Demo": "No", "COM3-DelayBetweenCharsMs": "0.0000", "470 Shutter-Volts": "0.0000", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "PRIME-PP 2 THRESHOLD": "125", "PRIME-PP 4 THRESHOLD": "75", "XYStage-Acceleration": "4.0000", "470 Shutter-Demo": "No", "PRIME-Name": "PMPCIECam00", "640-DA Device": "640 Shutter", "405 AO-Block voltage": "false", "ElapsedTime-ms": 1197356, "PRIME-Offset": "100", "445 AO-IOChannel": "Dev1/ao1", "640 AO-MaxVolts": "5.0000", "PositionIndex": 29, "PRIME-PP 1 ENABLED": "Yes", "COM1-BaudRate": "9600", "640-State": "0", "COM3-Verbose": "1", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "LudlController-TransmissionDelay": "0", "PRIME-FullWellCapacity": "62000", "PRIME-SMARTStreamingEnabled": "No", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "640 Shutter-Description": "NI DAC", "528-Description": "DA device that is used as a shutter", "COM4-Description": "Serial port driver (boost:asio)", "405 Shutter-Description": "NI DAC", "TE2000-Name": "TE2000", "528 Shutter-Description": "NI DAC", "COM9-Description": "Serial port driver (boost:asio)", "LudlController-Name": "LudlController", "445 Shutter-TriggerSequenceLength": "1000", "PRIME-SerialNumber": "A18B203008", "445 AO-Volts": "0.0000", "PRIME-X-dimension": "1200", "PRIME-ShutterCloseDelay": "0", "XLIGHT Disk slider-State": "2", "Position": "Default", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 AO-IOChannel": "Dev1/ao5", "520 AO-IOChannel": "Dev1/ao4", "528 AO-TriggerSequenceLength": "1000", "405 Shutter-MaxVolts": "5.0000", "470-DA Device": "470 Shutter", "PRIME-FTCapable": "No", "Core-ChannelGroup": "Channels", "555 AO-SupportsTriggering": "0", "PRIME-Y-dimension": "1200", "Frame": 0, "555 Shutter-Sequenceable": "1", "PRIME-Timing-ExposureTimeNs": "499998000.0000", "Core-ImageProcessor": "", "528-State": "0", "520 AO-Block voltage": "false", "XLIGHT Excitation Wheel-Label": "QUAD", "640 Shutter-Demo": "No", "COM3-StopBits": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "520 Shutter-IOChannel": "Dev1/ao11", "Width": 1200, "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-SupportsTriggering": "0", "640 AO-TriggerSampleRate": "100000", "520-Description": "DA device that is used as a shutter", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "405 Shutter-Sequenceable": "1", "405 Shutter-SupportsTriggering": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-TriggerSampleRate": "100000", "640 Shutter-MaxVolts": "5.0000", "555 AO-MaxVolts": "5.0000", "520 AO-TriggerSequenceLength": "1000", "COM3-BaudRate": "9600", "COM9-BaudRate": "9600", "Vincent-D3-Channel #": "Ch. #1", "PRIME-PMode": "Normal", "OpticalPath-Label": "Left(100)", "BitDepth": 16, "555 AO-TriggerSequenceLength": "1000", "445 Shutter-Description": "NI DAC", "405 AO-Sequenceable": "1", "PRIME-AcquisitionMethod": "Callbacks", "555 AO-Name": "AnalogIO", "PRIME-ShutterMode": "Pre-Exposure", "COM3-Handshaking": "Off", "445 AO-SupportsTriggering": "0", "ROI": "0-0-1200-1200", "COM9-Verbose": "1", "PRIME-Color": "OFF", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "PRIME-ShutterOpenDelay": "0", "COM1-Parity": "None", "Slice": 0, "SlicePosition": -50, "COM9-DelayBetweenCharsMs": "0.0000", "555-Name": "DA Shutter", "PIEZO-LudlSingleAxisName": "B", "XLIGHT Emission Wheel-Label": "585/11(T)", "640 Shutter-Name": "AnalogIO", "640 AO-Sequenceable": "1", "520 Shutter-Demo": "No", "528 Shutter-Demo": "No", "470-Name": "DA Shutter", "520-DA Device": "520 Shutter", "XYStage-Description": "Ludl XY stage driver adapter", "445 Shutter-MinVolts": "0.0000", "528 AO-TriggerSampleRate": "100000", "405 AO-MinVolts": "0.0000", "405 Shutter-TriggerSequenceLength": "1000", "Height": 1200, "555 AO-Block voltage": "false", "640 Shutter-Volts": "0.0000", "PixelType": "GRAY16", "445 Shutter-Block voltage": "false", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "520 Shutter-TriggerSequenceLength": "1000", "COM9-Name": "COM9", "Core-SLM": "", "555 AO-Volts": "4.5000", "555 Shutter-Block voltage": "false", "XYStage-Speed": "1800.0000", "520 AO-MaxVolts": "5.0000", "528 Shutter-SupportsTriggering": "0", "COM1-Description": "Serial port driver (boost:asio)", "PRIME-MetadataEnabled": "No", "520 Shutter-MinVolts": "0.0000", "470 Shutter-Sequenceable": "1", "PRIME-PreampDelay": "0", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-MinVolts": "0.0000", "ChannelIndex": 1, "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Core-AutoShutter": "1", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "Exposure-ms": 160, "PRIME-PP 2 ENABLED": "Yes", "640 Shutter-TriggerSampleRate": "100000", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "470 Shutter-IOChannel": "Dev1/ao10", "405 AO-Demo": "No", "PixelSizeUm": 1.1, "470 AO-Demo": "No", "470 Shutter-MaxVolts": "5.0000", "Vincent-D3-Name": "Vincent-D3", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FileName": "img_000000000_Il-T_000.tif", "LudlController-Reset": "Operate", "PRIME-Exposure": "160.00", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "445-Name": "DA Shutter", "555 Shutter-Name": "AnalogIO", "520 Shutter-TriggerSampleRate": "100000", "445 Shutter-IOChannel": "Dev1/ao9", "PRIME-TransposeCorrection": "0", "XYStage-StepSize": "0.1000", "445-Description": "DA device that is used as a shutter", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 AO-IOChannel": "Dev1/ao7", "520 Shutter-Name": "AnalogIO", "COM3-DataBits": "8", "640 AO-Volts": "0.0000", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "555 Shutter-IOChannel": "Dev1/ao13", "WaitInterval": null, "520 Shutter-Sequenceable": "1", "YPositionUm": -180, "445-State": "0", "445-DA Device": "445 Shutter", "PRIME-CircularBufferAutoSize": "ON", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "555 AO-TriggerSampleRate": "100000", "445 Shutter-Volts": "0.0000", "XLIGHT Hub-Port": "COM3", "Core-AutoFocus": "", "COM4-Handshaking": "Off", "528 Shutter-MinVolts": "0.0000", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "445 Shutter-Name": "AnalogIO", "528 Shutter-Sequenceable": "1", "XYStage-TransposeMirrorX": "0", "XYStage-TransposeMirrorY": "0", "PRIME-ReadoutRate": "100MHz 16bit", "XYStage-Name": "Stage", "640 AO-SupportsTriggering": "0", "640-Name": "DA Shutter", "640 Shutter-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "640 AO-Block voltage": "false", "445 Shutter-Sequenceable": "1", "470 AO-MaxVolts": "5.0000", "COM4-BaudRate": "9600", "470-Description": "DA device that is used as a shutter", "520 Shutter-Description": "NI DAC", "PRIME-CCDTemperature": "-14.8500", "Time": "2019-12-18 23:10:57 -0500", "640 AO-TriggerSequenceLength": "1000", "470 AO-Name": "AnalogIO", "XLIGHT Disk slider-Label": "Disk Pos 40um", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "Binning": "1x1", "TE2000-ModelType": "1", "COM3-AnswerTimeout": "500.0000", "555 Shutter-SupportsTriggering": "0", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "TE2000-Port": "COM4", "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Description": "NI DAC", "COM4-AnswerTimeout": "500.0000", "COM1-DelayBetweenCharsMs": "0.0000", "FilterBlock-Label": "Confocal", "445 AO-Name": "AnalogIO", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-Timing-ReadoutTimeNs": "24672000.0000", "528 AO-Demo": "No", "520 AO-Sequenceable": "1", "555-Description": "DA device that is used as a shutter", "405-DA Device": "405 Shutter", "405 AO-TriggerSequenceLength": "1000", "640 AO-MinVolts": "0.0000", "640 Shutter-Block voltage": "false", "FrameIndex": 0, "PRIME-Port": "CMOS", "555 AO-MinVolts": "0.0000", "COM3-Description": "Serial port driver (boost:asio)", "528 AO-Description": "NI DAC", "555 AO-IOChannel": "Dev1/ao6", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "555 AO-TriggerInputLine": "/Dev1/PFI0", "PRIME-ActualInterval-ms": "390.5200", "LudlController-Port": "COM9", "445 Shutter-Demo": "No", "PRIME-Trigger-Expose Out-Mux": "1", "SliceIndex": 0, "PRIME-CCDTemperatureSetPoint": "-15.0000", "405 Shutter-MinVolts": "0.0000", "PRIME-ExposeOutMode": "First Row", "AxisPositions": null, "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Volts": "5.0000", "528-Name": "DA Shutter", "555-State": "1", "XLIGHT Dichroic-Label": "QUAD", "445 AO-Sequenceable": "1", "405-Name": "DA Shutter", "405 AO-IOChannel": "Dev1/ao0", "Core-Camera": "PRIME", "Focus-Name": "Focus", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "405 Shutter-IOChannel": "Dev1/ao8", "640 AO-Demo": "No", "640 Shutter-SupportsTriggering": "0", "445 AO-Demo": "No", "555-DA Device": "555 Shutter", "470 Shutter-Description": "NI DAC", "528 AO-Volts": "0.0000", "COM1-DataBits": "8", "Vincent-D3-Address": "x", "520 AO-SupportsTriggering": "0", "405-Description": "DA device that is used as a shutter", "640 Shutter-MinVolts": "0.0000", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "405 AO-Name": "AnalogIO", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "640 AO-TriggerInputLine": "/Dev1/PFI0", "405 AO-TriggerInputLine": "/Dev1/PFI0", "640-Description": "DA device that is used as a shutter", "445 Shutter-TriggerSampleRate": "100000", "470 Shutter-TriggerSequenceLength": "1000", "COM1-StopBits": "1", "520 Shutter-SupportsTriggering": "0", "405-State": "0", "Core-Initialize": "1", "XLIGHT Emission Wheel-State": "3", "520 AO-Description": "NI DAC", "528 AO-TriggerInputLine": "/Dev1/PFI0", "445 Shutter-MaxVolts": "5.0000", "COM9-Handshaking": "Off", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "XLIGHT Spin motor-Label": "On (spinning)", "PRIME-PP 5 ENABLED": "No", "UUID": "6cb0d84c-d6b7-4599-a2d5-65c9b72130b3", "PRIME-TriggerMode": "Internal Trigger", "COM1-Handshaking": "Off", "PRIME-Timing-PostTriggerDelayNs": "49344000.0000", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "Focus-Position": "17741.5000", "528 Shutter-Block voltage": "false", "Channel": "Il-T", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "555 Shutter-Demo": "No", "405 Shutter-Demo": "No", "528 AO-Name": "AnalogIO", "520-State": "0", "Core-Galvo": "", "405 Shutter-TriggerSampleRate": "100000", "Focus-FocusStepSize_(nm)": "100", "PRIME-PixelType": "16bit", "COM4-DataBits": "8", "470 AO-IOChannel": "Dev1/ao2", "520 AO-MinVolts": "0.0000", "COM9-Parity": "None", "PRIME-CircularBufferEnabled": "ON", "PRIME-PP 6 Reset": "No", "640 AO-Description": "NI DAC", "Source": "PRIME", "445 AO-MinVolts": "0.0000", "PIEZO-Name": "Stage", "445 AO-Description": "NI DAC", "PRIME-CircularBufferFrameCount": "46", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "520 AO-Volts": "0.0000", "528 Shutter-TriggerSampleRate": "100000", "XPositionUm": 15712.1, "OpticalPath-State": "4", "PRIME-PP 3 THRESHOLD": "80", "PRIME-PVCAM Version": "3.7.1", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Gain": "1-HDR", "640 Shutter-TriggerSequenceLength": "1000", "528 AO-SupportsTriggering": "0", "445 AO-TriggerInputLine": "/Dev1/PFI0", "OpticalPath-Name": "OpticalPath", "PRIME-PP 4 ENABLED": "Yes", "PRIME-PVCAM Adapter Version": "1.3.29", "COM3-Name": "COM3", "405 AO-MaxVolts": "5.0000", "520 Shutter-Block voltage": "false", "PRIME-Timing-ClearingTimeNs": "49344000.0000", "Core-Focus": "PIEZO", "528 AO-Block voltage": "false", "TE2000-Description": "Nikon TE2000 hub", "528 AO-MinVolts": "0.0000", "COM4-Parity": "None", "Summary": { "Slices": 11, "ChContrastMin": [ 0, 0, 0, 0, 0, 0 ], "PixelAspect": 1, "SlicesFirst": true, "Interval_ms": 0, "Time": "2019-12-18 23:10:50 -0500", "Prefix": "seq_5", "InitialPositionList": [ { "Label": "Pos0", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.21], "Focus": [17741.5], "XYStage": [ 44542.200000000004, 31636.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos1", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.22], "Focus": [17741.4], "XYStage": [ 43801.100000000006, 31637.9 ] }, "GridColumnIndex": 0 }, { "Label": "Pos2", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.5], "XYStage": [ 38561.3, 32040.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos3", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.185], "Focus": [17741.3], "XYStage": [ 37698.4, 32041.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos4", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.16], "Focus": [17741.5], "XYStage": [ 32375.4, 31758.9 ] }, "GridColumnIndex": 0 }, { "Label": "Pos5", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.17000000000002], "Focus": [17741.3], "XYStage": [ 31770.7, 31758.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos6", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.4], "XYStage": [ 26536.800000000003, 31068.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos7", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.205], "Focus": [17741.4], "XYStage": [ 25420.2, 31068.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos8", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.85], "Focus": [17741.3], "XYStage": [ 21059, 31508 ] }, "GridColumnIndex": 0 }, { "Label": "Pos9", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.82], "Focus": [17741.3], "XYStage": [ 19731.9, 31506.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos10", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.79], "Focus": [17741.4], "XYStage": [ 14528.5, 32007.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos11", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.83], "Focus": [17741.2], "XYStage": [ 13830.300000000001, 32009.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos12", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.79], "Focus": [17741.3], "XYStage": [ 8775.300000000001, 32017.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos13", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.815], "Focus": [17741.3], "XYStage": [ 8109.200000000001, 32008.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos14", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.805], "Focus": [17741.3], "XYStage": [ 3483.3, 31908.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos15", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.82], "Focus": [17741.2], "XYStage": [ 2686.8, 31903.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos16", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [211.48000000000002], "Focus": [17741.1], "XYStage": [ -1978.9, 31973.600000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos17", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [211.51500000000001], "Focus": [17741.1], "XYStage": [ -2957.8, 31982.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos18", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.195], "Focus": [17741.5], "XYStage": [ 47747.4, -576.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos19", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.23000000000002], "Focus": [17741.6], "XYStage": [ 47053.9, -576.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos20", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.205], "Focus": [17741.4], "XYStage": [ 41871.200000000004, -479.8 ] }, "GridColumnIndex": 0 }, { "Label": "Pos21", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.4], "XYStage": [ 40882.600000000006, -392 ] }, "GridColumnIndex": 0 }, { "Label": "Pos22", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.24], "Focus": [17741.4], "XYStage": [ 35905.6, -135.3 ] }, "GridColumnIndex": 0 }, { "Label": "Pos23", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.195], "Focus": [17741.5], "XYStage": [ 34990.9, -132.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos24", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.46], "Focus": [17741.4], "XYStage": [ 29678.100000000002, -958.1 ] }, "GridColumnIndex": 0 }, { "Label": "Pos25", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.44], "Focus": [17741.5], "XYStage": [ 28983.600000000002, -939.4000000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos26", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.475], "Focus": [17741.5], "XYStage": [ 23019.7, -122 ] }, "GridColumnIndex": 0 }, { "Label": "Pos27", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.44], "Focus": [17741.7], "XYStage": [ 22428, -116.80000000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos28", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.46], "Focus": [17741.7], "XYStage": [ 16364.900000000001, -181.10000000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos29", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.48000000000002], "Focus": [17741.5], "XYStage": [ 15712.1, -180 ] }, "GridColumnIndex": 0 }, { "Label": "Pos30", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.45000000000002], "Focus": [17741.7], "XYStage": [ 10346.1, 77.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos31", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.49], "Focus": [17741.4], "XYStage": [ 9755.5, 85.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos32", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.475], "Focus": [17741.5], "XYStage": [ 4775.5, 293.90000000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos33", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.445], "Focus": [17741.4], "XYStage": [ 3954.9, 293.90000000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos34", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.455], "Focus": [17741.4], "XYStage": [ -1324.9, 545.6 ] }, "GridColumnIndex": 0 }, { "Label": "Pos35", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.445], "Focus": [17741.3], "XYStage": [ -2097, 576.6 ] }, "GridColumnIndex": 0 } ], "z-step_um": 10, "GridRow": 0, "BitDepth": 16, "Depth": 2, "Channels": 6, "Source": "Micro-Manager", "ChColors": [ -16711681, -256, -65281, -1, -1, -16776961 ], "MetadataVersion": 10, "KeepShutterOpenChannels": true, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536, 65536 ], "CustomIntervals_ms": [], "Height": 1200, "MicroManagerVersion": "1.4.23 20180219", "GridColumn": 0, "UUID": "67598d21-5db5-4842-ab60-f506f7b42c7c", "PixelType": "GRAY16", "Width": 1200, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\Yan\\20191218-YC113-S7S8-miseq", "Frames": 1, "Comment": "", "UserName": "Nikon", "PixelSize_um": 1.1, "ROI": [ 0, 0, 1200, 1200 ], "Date": "2019-12-18", "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC", "P-DAPI-high" ], "KeepShutterOpenSlices": true, "IJType": 1, "ComputerName": "ZadorConfocal2", "Positions": 36, "PositionIndex": 29, "CameraTimeout": 20000, "TimeFirst": false }, "640 AO-Name": "AnalogIO", "Vincent-D3-Command": "Close" }, "FrameKey-0-1-1": { "COM4-StopBits": "1", "528 AO-MaxVolts": "5.0000", "XLIGHT Excitation Wheel-State": "1", "470 Shutter-Name": "AnalogIO", "Core-Shutter": "555", "555 AO-Demo": "No", "XLIGHT Dichroic-State": "2", "XYStage-StepSize-Y": "0.1000", "ZPositionUm": 178.48000000000002, "XYStage-StepSize-X": "0.1000", "PRIME-FirmwareVersion": "20.28", "PRIME-ClearCycles": "2", "520-Name": "DA Shutter", "405 Shutter-Name": "AnalogIO", "PIEZO-StepSize": "0.0050", "PRIME-ClearMode": "Pre-Exposure", "470 AO-MinVolts": "0.0000", "405 AO-SupportsTriggering": "1", "CameraChannelIndex": 0, "XYStage-StartSpeed": "500.0000", "COM9-StopBits": "2", "405 AO-Description": "NI DAC", "COM4-DelayBetweenCharsMs": "0.0000", "445 AO-Block voltage": "false", "520 AO-TriggerInputLine": "/Dev1/PFI0", "555 AO-Description": "NI DAC", "520 AO-TriggerSampleRate": "100000", "COM4-Verbose": "1", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM4-Name": "COM4", "405 Shutter-Volts": "0.0000", "Vincent-D3-Port": "COM1", "PRIME-PP 4 MIN ADU AFFECTED": "200", "470 Shutter-MinVolts": "0.0000", "PRIME-PP 3 ENABLED": "Yes", "470 AO-Description": "NI DAC", "PRIME-Description": "PVCAM API device adapter", "520 Shutter-MaxVolts": "5.0000", "520 Shutter-Volts": "0.0000", "528 AO-Sequenceable": "1", "PRIME-Binning": "1x1", "555 Shutter-MaxVolts": "5.0000", "528 Shutter-TriggerSequenceLength": "1000", "405 Shutter-Block voltage": "false", "XLIGHT Spin motor-State": "1", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "Vincent-D3-Time to open (ms)": "35.0000", "Vincent-D3-Time to close (ms)": "35.0000", "445 AO-TriggerSampleRate": "100000", "640 Shutter-IOChannel": "Dev1/ao14", "555 AO-Sequenceable": "1", "520 AO-Name": "AnalogIO", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "470 AO-Block voltage": "false", "COM1-Verbose": "1", "COM9-AnswerTimeout": "10000.0000", "528 Shutter-Name": "AnalogIO", "PRIME-Trigger Timeout (secs)": "10", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "NextFrame": 0, "470 AO-TriggerSequenceLength": "1000", "PRIME-TransposeXY": "0", "445 AO-TriggerSequenceLength": "1000", "COM1-Name": "COM1", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "COM9-DataBits": "8", "COM1-AnswerTimeout": "500.0000", "Camera": "", "TE2000-Version": "V2.50-V2.50-V2.10", "528 Shutter-Volts": "0.0000", "XLIGHT Hub-Name": "XLIGHT Hub", "PIEZO-Description": "Ludl stage driver adapter", "PositionName": "Pos29", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSampleRate": "100000", "PRIME-FanSpeedSetpoint": "High", "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "405 AO-TriggerSampleRate": "100000", "470 Shutter-Block voltage": "false", "470-State": "0", "COM3-Parity": "None", "405 AO-Volts": "0.0000", "PRIME-TransposeMirrorY": "0", "PRIME-ChipName": "GS144BSI", "470 AO-Volts": "0.0000", "PRIME-TransposeMirrorX": "0", "445 Shutter-SupportsTriggering": "0", "470 Shutter-SupportsTriggering": "0", "XLIGHT Hub-Description": "XLIGHT hub", "PRIME-PreampOffLimit": "10000", "470 AO-Sequenceable": "1", "528 Shutter-MaxVolts": "5.0000", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "Core-XYStage": "XYStage", "470 AO-TriggerSampleRate": "100000", "PRIME-Timing-PreTriggerDelayNs": "24672000.0000", "528-DA Device": "528 Shutter", "Focus-Description": "Nikon TE2000 focus adapter", "FilterBlock-State": "5", "520 AO-Demo": "No", "COM3-DelayBetweenCharsMs": "0.0000", "470 Shutter-Volts": "0.0000", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "PRIME-PP 2 THRESHOLD": "125", "PRIME-PP 4 THRESHOLD": "75", "XYStage-Acceleration": "4.0000", "470 Shutter-Demo": "No", "PRIME-Name": "PMPCIECam00", "640-DA Device": "640 Shutter", "405 AO-Block voltage": "false", "ElapsedTime-ms": 1197818, "PRIME-Offset": "100", "445 AO-IOChannel": "Dev1/ao1", "640 AO-MaxVolts": "5.0000", "PositionIndex": 29, "PRIME-PP 1 ENABLED": "Yes", "COM1-BaudRate": "9600", "640-State": "0", "COM3-Verbose": "1", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "LudlController-TransmissionDelay": "0", "PRIME-FullWellCapacity": "62000", "PRIME-SMARTStreamingEnabled": "No", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "640 Shutter-Description": "NI DAC", "528-Description": "DA device that is used as a shutter", "COM4-Description": "Serial port driver (boost:asio)", "405 Shutter-Description": "NI DAC", "TE2000-Name": "TE2000", "528 Shutter-Description": "NI DAC", "COM9-Description": "Serial port driver (boost:asio)", "LudlController-Name": "LudlController", "445 Shutter-TriggerSequenceLength": "1000", "PRIME-SerialNumber": "A18B203008", "445 AO-Volts": "0.0000", "PRIME-X-dimension": "1200", "PRIME-ShutterCloseDelay": "0", "XLIGHT Disk slider-State": "2", "Position": "Default", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 AO-IOChannel": "Dev1/ao5", "520 AO-IOChannel": "Dev1/ao4", "528 AO-TriggerSequenceLength": "1000", "405 Shutter-MaxVolts": "5.0000", "470-DA Device": "470 Shutter", "PRIME-FTCapable": "No", "Core-ChannelGroup": "Channels", "555 AO-SupportsTriggering": "0", "PRIME-Y-dimension": "1200", "Frame": 0, "555 Shutter-Sequenceable": "1", "PRIME-Timing-ExposureTimeNs": "499998000.0000", "Core-ImageProcessor": "", "528-State": "0", "520 AO-Block voltage": "false", "XLIGHT Excitation Wheel-Label": "QUAD", "640 Shutter-Demo": "No", "COM3-StopBits": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "520 Shutter-IOChannel": "Dev1/ao11", "Width": 1200, "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-SupportsTriggering": "0", "640 AO-TriggerSampleRate": "100000", "520-Description": "DA device that is used as a shutter", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "405 Shutter-Sequenceable": "1", "405 Shutter-SupportsTriggering": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-TriggerSampleRate": "100000", "640 Shutter-MaxVolts": "5.0000", "555 AO-MaxVolts": "5.0000", "520 AO-TriggerSequenceLength": "1000", "COM3-BaudRate": "9600", "COM9-BaudRate": "9600", "Vincent-D3-Channel #": "Ch. #1", "PRIME-PMode": "Normal", "OpticalPath-Label": "Left(100)", "BitDepth": 16, "555 AO-TriggerSequenceLength": "1000", "445 Shutter-Description": "NI DAC", "405 AO-Sequenceable": "1", "PRIME-AcquisitionMethod": "Callbacks", "555 AO-Name": "AnalogIO", "PRIME-ShutterMode": "Pre-Exposure", "COM3-Handshaking": "Off", "445 AO-SupportsTriggering": "0", "ROI": "0-0-1200-1200", "COM9-Verbose": "1", "PRIME-Color": "OFF", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "PRIME-ShutterOpenDelay": "0", "COM1-Parity": "None", "Slice": 1, "SlicePosition": -40, "COM9-DelayBetweenCharsMs": "0.0000", "555-Name": "DA Shutter", "PIEZO-LudlSingleAxisName": "B", "XLIGHT Emission Wheel-Label": "585/11(T)", "640 Shutter-Name": "AnalogIO", "640 AO-Sequenceable": "1", "520 Shutter-Demo": "No", "528 Shutter-Demo": "No", "470-Name": "DA Shutter", "520-DA Device": "520 Shutter", "XYStage-Description": "Ludl XY stage driver adapter", "445 Shutter-MinVolts": "0.0000", "528 AO-TriggerSampleRate": "100000", "405 AO-MinVolts": "0.0000", "405 Shutter-TriggerSequenceLength": "1000", "Height": 1200, "555 AO-Block voltage": "false", "640 Shutter-Volts": "0.0000", "PixelType": "GRAY16", "445 Shutter-Block voltage": "false", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "520 Shutter-TriggerSequenceLength": "1000", "COM9-Name": "COM9", "Core-SLM": "", "555 AO-Volts": "4.5000", "555 Shutter-Block voltage": "false", "XYStage-Speed": "1800.0000", "520 AO-MaxVolts": "5.0000", "528 Shutter-SupportsTriggering": "0", "COM1-Description": "Serial port driver (boost:asio)", "PRIME-MetadataEnabled": "No", "520 Shutter-MinVolts": "0.0000", "470 Shutter-Sequenceable": "1", "PRIME-PreampDelay": "0", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-MinVolts": "0.0000", "ChannelIndex": 1, "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Core-AutoShutter": "1", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "Exposure-ms": 160, "PRIME-PP 2 ENABLED": "Yes", "640 Shutter-TriggerSampleRate": "100000", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "470 Shutter-IOChannel": "Dev1/ao10", "405 AO-Demo": "No", "PixelSizeUm": 1.1, "470 AO-Demo": "No", "470 Shutter-MaxVolts": "5.0000", "Vincent-D3-Name": "Vincent-D3", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FileName": "img_000000000_Il-T_001.tif", "LudlController-Reset": "Operate", "PRIME-Exposure": "160.00", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "445-Name": "DA Shutter", "555 Shutter-Name": "AnalogIO", "520 Shutter-TriggerSampleRate": "100000", "445 Shutter-IOChannel": "Dev1/ao9", "PRIME-TransposeCorrection": "0", "XYStage-StepSize": "0.1000", "445-Description": "DA device that is used as a shutter", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 AO-IOChannel": "Dev1/ao7", "520 Shutter-Name": "AnalogIO", "COM3-DataBits": "8", "640 AO-Volts": "0.0000", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "555 Shutter-IOChannel": "Dev1/ao13", "WaitInterval": null, "520 Shutter-Sequenceable": "1", "YPositionUm": -180, "445-State": "0", "445-DA Device": "445 Shutter", "PRIME-CircularBufferAutoSize": "ON", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "555 AO-TriggerSampleRate": "100000", "445 Shutter-Volts": "0.0000", "XLIGHT Hub-Port": "COM3", "Core-AutoFocus": "", "COM4-Handshaking": "Off", "528 Shutter-MinVolts": "0.0000", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "445 Shutter-Name": "AnalogIO", "528 Shutter-Sequenceable": "1", "XYStage-TransposeMirrorX": "0", "XYStage-TransposeMirrorY": "0", "PRIME-ReadoutRate": "100MHz 16bit", "XYStage-Name": "Stage", "640 AO-SupportsTriggering": "0", "640-Name": "DA Shutter", "640 Shutter-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "640 AO-Block voltage": "false", "445 Shutter-Sequenceable": "1", "470 AO-MaxVolts": "5.0000", "COM4-BaudRate": "9600", "470-Description": "DA device that is used as a shutter", "520 Shutter-Description": "NI DAC", "PRIME-CCDTemperature": "-14.8500", "Time": "2019-12-18 23:10:58 -0500", "640 AO-TriggerSequenceLength": "1000", "470 AO-Name": "AnalogIO", "XLIGHT Disk slider-Label": "Disk Pos 40um", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "Binning": "1x1", "TE2000-ModelType": "1", "COM3-AnswerTimeout": "500.0000", "555 Shutter-SupportsTriggering": "0", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "TE2000-Port": "COM4", "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Description": "NI DAC", "COM4-AnswerTimeout": "500.0000", "COM1-DelayBetweenCharsMs": "0.0000", "FilterBlock-Label": "Confocal", "445 AO-Name": "AnalogIO", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-Timing-ReadoutTimeNs": "24672000.0000", "528 AO-Demo": "No", "520 AO-Sequenceable": "1", "555-Description": "DA device that is used as a shutter", "405-DA Device": "405 Shutter", "405 AO-TriggerSequenceLength": "1000", "640 AO-MinVolts": "0.0000", "640 Shutter-Block voltage": "false", "FrameIndex": 0, "PRIME-Port": "CMOS", "555 AO-MinVolts": "0.0000", "COM3-Description": "Serial port driver (boost:asio)", "528 AO-Description": "NI DAC", "555 AO-IOChannel": "Dev1/ao6", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "555 AO-TriggerInputLine": "/Dev1/PFI0", "PRIME-ActualInterval-ms": "390.5200", "LudlController-Port": "COM9", "445 Shutter-Demo": "No", "PRIME-Trigger-Expose Out-Mux": "1", "SliceIndex": 1, "PRIME-CCDTemperatureSetPoint": "-15.0000", "405 Shutter-MinVolts": "0.0000", "PRIME-ExposeOutMode": "First Row", "AxisPositions": null, "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Volts": "5.0000", "528-Name": "DA Shutter", "555-State": "1", "XLIGHT Dichroic-Label": "QUAD", "445 AO-Sequenceable": "1", "405-Name": "DA Shutter", "405 AO-IOChannel": "Dev1/ao0", "Core-Camera": "PRIME", "Focus-Name": "Focus", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "405 Shutter-IOChannel": "Dev1/ao8", "640 AO-Demo": "No", "640 Shutter-SupportsTriggering": "0", "445 AO-Demo": "No", "555-DA Device": "555 Shutter", "470 Shutter-Description": "NI DAC", "528 AO-Volts": "0.0000", "COM1-DataBits": "8", "Vincent-D3-Address": "x", "520 AO-SupportsTriggering": "0", "405-Description": "DA device that is used as a shutter", "640 Shutter-MinVolts": "0.0000", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "405 AO-Name": "AnalogIO", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "640 AO-TriggerInputLine": "/Dev1/PFI0", "405 AO-TriggerInputLine": "/Dev1/PFI0", "640-Description": "DA device that is used as a shutter", "445 Shutter-TriggerSampleRate": "100000", "470 Shutter-TriggerSequenceLength": "1000", "COM1-StopBits": "1", "520 Shutter-SupportsTriggering": "0", "405-State": "0", "Core-Initialize": "1", "XLIGHT Emission Wheel-State": "3", "520 AO-Description": "NI DAC", "528 AO-TriggerInputLine": "/Dev1/PFI0", "445 Shutter-MaxVolts": "5.0000", "COM9-Handshaking": "Off", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "XLIGHT Spin motor-Label": "On (spinning)", "PRIME-PP 5 ENABLED": "No", "UUID": "86a7be5c-4519-40a9-b8f3-023be6a6f6cf", "PRIME-TriggerMode": "Internal Trigger", "COM1-Handshaking": "Off", "PRIME-Timing-PostTriggerDelayNs": "49344000.0000", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "Focus-Position": "17741.5000", "528 Shutter-Block voltage": "false", "Channel": "Il-T", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "555 Shutter-Demo": "No", "405 Shutter-Demo": "No", "528 AO-Name": "AnalogIO", "520-State": "0", "Core-Galvo": "", "405 Shutter-TriggerSampleRate": "100000", "Focus-FocusStepSize_(nm)": "100", "PRIME-PixelType": "16bit", "COM4-DataBits": "8", "470 AO-IOChannel": "Dev1/ao2", "520 AO-MinVolts": "0.0000", "COM9-Parity": "None", "PRIME-CircularBufferEnabled": "ON", "PRIME-PP 6 Reset": "No", "640 AO-Description": "NI DAC", "Source": "PRIME", "445 AO-MinVolts": "0.0000", "PIEZO-Name": "Stage", "445 AO-Description": "NI DAC", "PRIME-CircularBufferFrameCount": "46", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "520 AO-Volts": "0.0000", "528 Shutter-TriggerSampleRate": "100000", "XPositionUm": 15712.1, "OpticalPath-State": "4", "PRIME-PP 3 THRESHOLD": "80", "PRIME-PVCAM Version": "3.7.1", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Gain": "1-HDR", "640 Shutter-TriggerSequenceLength": "1000", "528 AO-SupportsTriggering": "0", "445 AO-TriggerInputLine": "/Dev1/PFI0", "OpticalPath-Name": "OpticalPath", "PRIME-PP 4 ENABLED": "Yes", "PRIME-PVCAM Adapter Version": "1.3.29", "COM3-Name": "COM3", "405 AO-MaxVolts": "5.0000", "520 Shutter-Block voltage": "false", "PRIME-Timing-ClearingTimeNs": "49344000.0000", "Core-Focus": "PIEZO", "528 AO-Block voltage": "false", "TE2000-Description": "Nikon TE2000 hub", "528 AO-MinVolts": "0.0000", "COM4-Parity": "None", "Summary": { "Slices": 11, "ChContrastMin": [ 0, 0, 0, 0, 0, 0 ], "PixelAspect": 1, "SlicesFirst": true, "Interval_ms": 0, "Time": "2019-12-18 23:10:50 -0500", "Prefix": "seq_5", "InitialPositionList": [ { "Label": "Pos0", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.21], "Focus": [17741.5], "XYStage": [ 44542.200000000004, 31636.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos1", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.22], "Focus": [17741.4], "XYStage": [ 43801.100000000006, 31637.9 ] }, "GridColumnIndex": 0 }, { "Label": "Pos2", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.5], "XYStage": [ 38561.3, 32040.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos3", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.185], "Focus": [17741.3], "XYStage": [ 37698.4, 32041.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos4", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.16], "Focus": [17741.5], "XYStage": [ 32375.4, 31758.9 ] }, "GridColumnIndex": 0 }, { "Label": "Pos5", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.17000000000002], "Focus": [17741.3], "XYStage": [ 31770.7, 31758.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos6", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.4], "XYStage": [ 26536.800000000003, 31068.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos7", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.205], "Focus": [17741.4], "XYStage": [ 25420.2, 31068.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos8", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.85], "Focus": [17741.3], "XYStage": [ 21059, 31508 ] }, "GridColumnIndex": 0 }, { "Label": "Pos9", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.82], "Focus": [17741.3], "XYStage": [ 19731.9, 31506.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos10", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.79], "Focus": [17741.4], "XYStage": [ 14528.5, 32007.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos11", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.83], "Focus": [17741.2], "XYStage": [ 13830.300000000001, 32009.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos12", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.79], "Focus": [17741.3], "XYStage": [ 8775.300000000001, 32017.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos13", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.815], "Focus": [17741.3], "XYStage": [ 8109.200000000001, 32008.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos14", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.805], "Focus": [17741.3], "XYStage": [ 3483.3, 31908.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos15", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.82], "Focus": [17741.2], "XYStage": [ 2686.8, 31903.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos16", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [211.48000000000002], "Focus": [17741.1], "XYStage": [ -1978.9, 31973.600000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos17", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [211.51500000000001], "Focus": [17741.1], "XYStage": [ -2957.8, 31982.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos18", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.195], "Focus": [17741.5], "XYStage": [ 47747.4, -576.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos19", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.23000000000002], "Focus": [17741.6], "XYStage": [ 47053.9, -576.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos20", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.205], "Focus": [17741.4], "XYStage": [ 41871.200000000004, -479.8 ] }, "GridColumnIndex": 0 }, { "Label": "Pos21", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.4], "XYStage": [ 40882.600000000006, -392 ] }, "GridColumnIndex": 0 }, { "Label": "Pos22", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.24], "Focus": [17741.4], "XYStage": [ 35905.6, -135.3 ] }, "GridColumnIndex": 0 }, { "Label": "Pos23", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.195], "Focus": [17741.5], "XYStage": [ 34990.9, -132.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos24", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.46], "Focus": [17741.4], "XYStage": [ 29678.100000000002, -958.1 ] }, "GridColumnIndex": 0 }, { "Label": "Pos25", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.44], "Focus": [17741.5], "XYStage": [ 28983.600000000002, -939.4000000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos26", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.475], "Focus": [17741.5], "XYStage": [ 23019.7, -122 ] }, "GridColumnIndex": 0 }, { "Label": "Pos27", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.44], "Focus": [17741.7], "XYStage": [ 22428, -116.80000000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos28", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.46], "Focus": [17741.7], "XYStage": [ 16364.900000000001, -181.10000000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos29", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.48000000000002], "Focus": [17741.5], "XYStage": [ 15712.1, -180 ] }, "GridColumnIndex": 0 }, { "Label": "Pos30", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.45000000000002], "Focus": [17741.7], "XYStage": [ 10346.1, 77.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos31", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.49], "Focus": [17741.4], "XYStage": [ 9755.5, 85.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos32", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.475], "Focus": [17741.5], "XYStage": [ 4775.5, 293.90000000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos33", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.445], "Focus": [17741.4], "XYStage": [ 3954.9, 293.90000000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos34", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.455], "Focus": [17741.4], "XYStage": [ -1324.9, 545.6 ] }, "GridColumnIndex": 0 }, { "Label": "Pos35", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.445], "Focus": [17741.3], "XYStage": [ -2097, 576.6 ] }, "GridColumnIndex": 0 } ], "z-step_um": 10, "GridRow": 0, "BitDepth": 16, "Depth": 2, "Channels": 6, "Source": "Micro-Manager", "ChColors": [ -16711681, -256, -65281, -1, -1, -16776961 ], "MetadataVersion": 10, "KeepShutterOpenChannels": true, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536, 65536 ], "CustomIntervals_ms": [], "Height": 1200, "MicroManagerVersion": "1.4.23 20180219", "GridColumn": 0, "UUID": "67598d21-5db5-4842-ab60-f506f7b42c7c", "PixelType": "GRAY16", "Width": 1200, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\Yan\\20191218-YC113-S7S8-miseq", "Frames": 1, "Comment": "", "UserName": "Nikon", "PixelSize_um": 1.1, "ROI": [ 0, 0, 1200, 1200 ], "Date": "2019-12-18", "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC", "P-DAPI-high" ], "KeepShutterOpenSlices": true, "IJType": 1, "ComputerName": "ZadorConfocal2", "Positions": 36, "PositionIndex": 29, "CameraTimeout": 20000, "TimeFirst": false }, "640 AO-Name": "AnalogIO", "Vincent-D3-Command": "Close" }, "FrameKey-0-1-2": { "COM4-StopBits": "1", "528 AO-MaxVolts": "5.0000", "XLIGHT Excitation Wheel-State": "1", "470 Shutter-Name": "AnalogIO", "Core-Shutter": "555", "555 AO-Demo": "No", "XLIGHT Dichroic-State": "2", "XYStage-StepSize-Y": "0.1000", "ZPositionUm": 188.48000000000002, "XYStage-StepSize-X": "0.1000", "PRIME-FirmwareVersion": "20.28", "PRIME-ClearCycles": "2", "520-Name": "DA Shutter", "405 Shutter-Name": "AnalogIO", "PIEZO-StepSize": "0.0050", "PRIME-ClearMode": "Pre-Exposure", "470 AO-MinVolts": "0.0000", "405 AO-SupportsTriggering": "1", "CameraChannelIndex": 0, "XYStage-StartSpeed": "500.0000", "COM9-StopBits": "2", "405 AO-Description": "NI DAC", "COM4-DelayBetweenCharsMs": "0.0000", "445 AO-Block voltage": "false", "520 AO-TriggerInputLine": "/Dev1/PFI0", "555 AO-Description": "NI DAC", "520 AO-TriggerSampleRate": "100000", "COM4-Verbose": "1", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM4-Name": "COM4", "405 Shutter-Volts": "0.0000", "Vincent-D3-Port": "COM1", "PRIME-PP 4 MIN ADU AFFECTED": "200", "470 Shutter-MinVolts": "0.0000", "PRIME-PP 3 ENABLED": "Yes", "470 AO-Description": "NI DAC", "PRIME-Description": "PVCAM API device adapter", "520 Shutter-MaxVolts": "5.0000", "520 Shutter-Volts": "0.0000", "528 AO-Sequenceable": "1", "PRIME-Binning": "1x1", "555 Shutter-MaxVolts": "5.0000", "528 Shutter-TriggerSequenceLength": "1000", "405 Shutter-Block voltage": "false", "XLIGHT Spin motor-State": "1", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "Vincent-D3-Time to open (ms)": "35.0000", "Vincent-D3-Time to close (ms)": "35.0000", "445 AO-TriggerSampleRate": "100000", "640 Shutter-IOChannel": "Dev1/ao14", "555 AO-Sequenceable": "1", "520 AO-Name": "AnalogIO", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "470 AO-Block voltage": "false", "COM1-Verbose": "1", "COM9-AnswerTimeout": "10000.0000", "528 Shutter-Name": "AnalogIO", "PRIME-Trigger Timeout (secs)": "10", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "NextFrame": 0, "470 AO-TriggerSequenceLength": "1000", "PRIME-TransposeXY": "0", "445 AO-TriggerSequenceLength": "1000", "COM1-Name": "COM1", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "COM9-DataBits": "8", "COM1-AnswerTimeout": "500.0000", "Camera": "", "TE2000-Version": "V2.50-V2.50-V2.10", "528 Shutter-Volts": "0.0000", "XLIGHT Hub-Name": "XLIGHT Hub", "PIEZO-Description": "Ludl stage driver adapter", "PositionName": "Pos29", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSampleRate": "100000", "PRIME-FanSpeedSetpoint": "High", "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "405 AO-TriggerSampleRate": "100000", "470 Shutter-Block voltage": "false", "470-State": "0", "COM3-Parity": "None", "405 AO-Volts": "0.0000", "PRIME-TransposeMirrorY": "0", "PRIME-ChipName": "GS144BSI", "470 AO-Volts": "0.0000", "PRIME-TransposeMirrorX": "0", "445 Shutter-SupportsTriggering": "0", "470 Shutter-SupportsTriggering": "0", "XLIGHT Hub-Description": "XLIGHT hub", "PRIME-PreampOffLimit": "10000", "470 AO-Sequenceable": "1", "528 Shutter-MaxVolts": "5.0000", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "Core-XYStage": "XYStage", "470 AO-TriggerSampleRate": "100000", "PRIME-Timing-PreTriggerDelayNs": "24672000.0000", "528-DA Device": "528 Shutter", "Focus-Description": "Nikon TE2000 focus adapter", "FilterBlock-State": "5", "520 AO-Demo": "No", "COM3-DelayBetweenCharsMs": "0.0000", "470 Shutter-Volts": "0.0000", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "PRIME-PP 2 THRESHOLD": "125", "PRIME-PP 4 THRESHOLD": "75", "XYStage-Acceleration": "4.0000", "470 Shutter-Demo": "No", "PRIME-Name": "PMPCIECam00", "640-DA Device": "640 Shutter", "405 AO-Block voltage": "false", "ElapsedTime-ms": 1198260, "PRIME-Offset": "100", "445 AO-IOChannel": "Dev1/ao1", "640 AO-MaxVolts": "5.0000", "PositionIndex": 29, "PRIME-PP 1 ENABLED": "Yes", "COM1-BaudRate": "9600", "640-State": "0", "COM3-Verbose": "1", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "LudlController-TransmissionDelay": "0", "PRIME-FullWellCapacity": "62000", "PRIME-SMARTStreamingEnabled": "No", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "640 Shutter-Description": "NI DAC", "528-Description": "DA device that is used as a shutter", "COM4-Description": "Serial port driver (boost:asio)", "405 Shutter-Description": "NI DAC", "TE2000-Name": "TE2000", "528 Shutter-Description": "NI DAC", "COM9-Description": "Serial port driver (boost:asio)", "LudlController-Name": "LudlController", "445 Shutter-TriggerSequenceLength": "1000", "PRIME-SerialNumber": "A18B203008", "445 AO-Volts": "0.0000", "PRIME-X-dimension": "1200", "PRIME-ShutterCloseDelay": "0", "XLIGHT Disk slider-State": "2", "Position": "Default", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 AO-IOChannel": "Dev1/ao5", "520 AO-IOChannel": "Dev1/ao4", "528 AO-TriggerSequenceLength": "1000", "405 Shutter-MaxVolts": "5.0000", "470-DA Device": "470 Shutter", "PRIME-FTCapable": "No", "Core-ChannelGroup": "Channels", "555 AO-SupportsTriggering": "0", "PRIME-Y-dimension": "1200", "Frame": 0, "555 Shutter-Sequenceable": "1", "PRIME-Timing-ExposureTimeNs": "499998000.0000", "Core-ImageProcessor": "", "528-State": "0", "520 AO-Block voltage": "false", "XLIGHT Excitation Wheel-Label": "QUAD", "640 Shutter-Demo": "No", "COM3-StopBits": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "520 Shutter-IOChannel": "Dev1/ao11", "Width": 1200, "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-SupportsTriggering": "0", "640 AO-TriggerSampleRate": "100000", "520-Description": "DA device that is used as a shutter", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "405 Shutter-Sequenceable": "1", "405 Shutter-SupportsTriggering": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-TriggerSampleRate": "100000", "640 Shutter-MaxVolts": "5.0000", "555 AO-MaxVolts": "5.0000", "520 AO-TriggerSequenceLength": "1000", "COM3-BaudRate": "9600", "COM9-BaudRate": "9600", "Vincent-D3-Channel #": "Ch. #1", "PRIME-PMode": "Normal", "OpticalPath-Label": "Left(100)", "BitDepth": 16, "555 AO-TriggerSequenceLength": "1000", "445 Shutter-Description": "NI DAC", "405 AO-Sequenceable": "1", "PRIME-AcquisitionMethod": "Callbacks", "555 AO-Name": "AnalogIO", "PRIME-ShutterMode": "Pre-Exposure", "COM3-Handshaking": "Off", "445 AO-SupportsTriggering": "0", "ROI": "0-0-1200-1200", "COM9-Verbose": "1", "PRIME-Color": "OFF", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "PRIME-ShutterOpenDelay": "0", "COM1-Parity": "None", "Slice": 2, "SlicePosition": -30, "COM9-DelayBetweenCharsMs": "0.0000", "555-Name": "DA Shutter", "PIEZO-LudlSingleAxisName": "B", "XLIGHT Emission Wheel-Label": "585/11(T)", "640 Shutter-Name": "AnalogIO", "640 AO-Sequenceable": "1", "520 Shutter-Demo": "No", "528 Shutter-Demo": "No", "470-Name": "DA Shutter", "520-DA Device": "520 Shutter", "XYStage-Description": "Ludl XY stage driver adapter", "445 Shutter-MinVolts": "0.0000", "528 AO-TriggerSampleRate": "100000", "405 AO-MinVolts": "0.0000", "405 Shutter-TriggerSequenceLength": "1000", "Height": 1200, "555 AO-Block voltage": "false", "640 Shutter-Volts": "0.0000", "PixelType": "GRAY16", "445 Shutter-Block voltage": "false", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "520 Shutter-TriggerSequenceLength": "1000", "COM9-Name": "COM9", "Core-SLM": "", "555 AO-Volts": "4.5000", "555 Shutter-Block voltage": "false", "XYStage-Speed": "1800.0000", "520 AO-MaxVolts": "5.0000", "528 Shutter-SupportsTriggering": "0", "COM1-Description": "Serial port driver (boost:asio)", "PRIME-MetadataEnabled": "No", "520 Shutter-MinVolts": "0.0000", "470 Shutter-Sequenceable": "1", "PRIME-PreampDelay": "0", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-MinVolts": "0.0000", "ChannelIndex": 1, "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Core-AutoShutter": "1", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "Exposure-ms": 160, "PRIME-PP 2 ENABLED": "Yes", "640 Shutter-TriggerSampleRate": "100000", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "470 Shutter-IOChannel": "Dev1/ao10", "405 AO-Demo": "No", "PixelSizeUm": 1.1, "470 AO-Demo": "No", "470 Shutter-MaxVolts": "5.0000", "Vincent-D3-Name": "Vincent-D3", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FileName": "img_000000000_Il-T_002.tif", "LudlController-Reset": "Operate", "PRIME-Exposure": "160.00", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "445-Name": "DA Shutter", "555 Shutter-Name": "AnalogIO", "520 Shutter-TriggerSampleRate": "100000", "445 Shutter-IOChannel": "Dev1/ao9", "PRIME-TransposeCorrection": "0", "XYStage-StepSize": "0.1000", "445-Description": "DA device that is used as a shutter", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 AO-IOChannel": "Dev1/ao7", "520 Shutter-Name": "AnalogIO", "COM3-DataBits": "8", "640 AO-Volts": "0.0000", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "555 Shutter-IOChannel": "Dev1/ao13", "WaitInterval": null, "520 Shutter-Sequenceable": "1", "YPositionUm": -180, "445-State": "0", "445-DA Device": "445 Shutter", "PRIME-CircularBufferAutoSize": "ON", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "555 AO-TriggerSampleRate": "100000", "445 Shutter-Volts": "0.0000", "XLIGHT Hub-Port": "COM3", "Core-AutoFocus": "", "COM4-Handshaking": "Off", "528 Shutter-MinVolts": "0.0000", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "445 Shutter-Name": "AnalogIO", "528 Shutter-Sequenceable": "1", "XYStage-TransposeMirrorX": "0", "XYStage-TransposeMirrorY": "0", "PRIME-ReadoutRate": "100MHz 16bit", "XYStage-Name": "Stage", "640 AO-SupportsTriggering": "0", "640-Name": "DA Shutter", "640 Shutter-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "640 AO-Block voltage": "false", "445 Shutter-Sequenceable": "1", "470 AO-MaxVolts": "5.0000", "COM4-BaudRate": "9600", "470-Description": "DA device that is used as a shutter", "520 Shutter-Description": "NI DAC", "PRIME-CCDTemperature": "-14.8500", "Time": "2019-12-18 23:10:58 -0500", "640 AO-TriggerSequenceLength": "1000", "470 AO-Name": "AnalogIO", "XLIGHT Disk slider-Label": "Disk Pos 40um", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "Binning": "1x1", "TE2000-ModelType": "1", "COM3-AnswerTimeout": "500.0000", "555 Shutter-SupportsTriggering": "0", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "TE2000-Port": "COM4", "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Description": "NI DAC", "COM4-AnswerTimeout": "500.0000", "COM1-DelayBetweenCharsMs": "0.0000", "FilterBlock-Label": "Confocal", "445 AO-Name": "AnalogIO", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-Timing-ReadoutTimeNs": "24672000.0000", "528 AO-Demo": "No", "520 AO-Sequenceable": "1", "555-Description": "DA device that is used as a shutter", "405-DA Device": "405 Shutter", "405 AO-TriggerSequenceLength": "1000", "640 AO-MinVolts": "0.0000", "640 Shutter-Block voltage": "false", "FrameIndex": 0, "PRIME-Port": "CMOS", "555 AO-MinVolts": "0.0000", "COM3-Description": "Serial port driver (boost:asio)", "528 AO-Description": "NI DAC", "555 AO-IOChannel": "Dev1/ao6", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "555 AO-TriggerInputLine": "/Dev1/PFI0", "PRIME-ActualInterval-ms": "390.5200", "LudlController-Port": "COM9", "445 Shutter-Demo": "No", "PRIME-Trigger-Expose Out-Mux": "1", "SliceIndex": 2, "PRIME-CCDTemperatureSetPoint": "-15.0000", "405 Shutter-MinVolts": "0.0000", "PRIME-ExposeOutMode": "First Row", "AxisPositions": null, "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Volts": "5.0000", "528-Name": "DA Shutter", "555-State": "1", "XLIGHT Dichroic-Label": "QUAD", "445 AO-Sequenceable": "1", "405-Name": "DA Shutter", "405 AO-IOChannel": "Dev1/ao0", "Core-Camera": "PRIME", "Focus-Name": "Focus", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "405 Shutter-IOChannel": "Dev1/ao8", "640 AO-Demo": "No", "640 Shutter-SupportsTriggering": "0", "445 AO-Demo": "No", "555-DA Device": "555 Shutter", "470 Shutter-Description": "NI DAC", "528 AO-Volts": "0.0000", "COM1-DataBits": "8", "Vincent-D3-Address": "x", "520 AO-SupportsTriggering": "0", "405-Description": "DA device that is used as a shutter", "640 Shutter-MinVolts": "0.0000", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "405 AO-Name": "AnalogIO", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "640 AO-TriggerInputLine": "/Dev1/PFI0", "405 AO-TriggerInputLine": "/Dev1/PFI0", "640-Description": "DA device that is used as a shutter", "445 Shutter-TriggerSampleRate": "100000", "470 Shutter-TriggerSequenceLength": "1000", "COM1-StopBits": "1", "520 Shutter-SupportsTriggering": "0", "405-State": "0", "Core-Initialize": "1", "XLIGHT Emission Wheel-State": "3", "520 AO-Description": "NI DAC", "528 AO-TriggerInputLine": "/Dev1/PFI0", "445 Shutter-MaxVolts": "5.0000", "COM9-Handshaking": "Off", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "XLIGHT Spin motor-Label": "On (spinning)", "PRIME-PP 5 ENABLED": "No", "UUID": "e11e0c4e-4967-48d0-84b2-0d78924ef56c", "PRIME-TriggerMode": "Internal Trigger", "COM1-Handshaking": "Off", "PRIME-Timing-PostTriggerDelayNs": "49344000.0000", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "Focus-Position": "17741.5000", "528 Shutter-Block voltage": "false", "Channel": "Il-T", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "555 Shutter-Demo": "No", "405 Shutter-Demo": "No", "528 AO-Name": "AnalogIO", "520-State": "0", "Core-Galvo": "", "405 Shutter-TriggerSampleRate": "100000", "Focus-FocusStepSize_(nm)": "100", "PRIME-PixelType": "16bit", "COM4-DataBits": "8", "470 AO-IOChannel": "Dev1/ao2", "520 AO-MinVolts": "0.0000", "COM9-Parity": "None", "PRIME-CircularBufferEnabled": "ON", "PRIME-PP 6 Reset": "No", "640 AO-Description": "NI DAC", "Source": "PRIME", "445 AO-MinVolts": "0.0000", "PIEZO-Name": "Stage", "445 AO-Description": "NI DAC", "PRIME-CircularBufferFrameCount": "46", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "520 AO-Volts": "0.0000", "528 Shutter-TriggerSampleRate": "100000", "XPositionUm": 15712.1, "OpticalPath-State": "4", "PRIME-PP 3 THRESHOLD": "80", "PRIME-PVCAM Version": "3.7.1", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Gain": "1-HDR", "640 Shutter-TriggerSequenceLength": "1000", "528 AO-SupportsTriggering": "0", "445 AO-TriggerInputLine": "/Dev1/PFI0", "OpticalPath-Name": "OpticalPath", "PRIME-PP 4 ENABLED": "Yes", "PRIME-PVCAM Adapter Version": "1.3.29", "COM3-Name": "COM3", "405 AO-MaxVolts": "5.0000", "520 Shutter-Block voltage": "false", "PRIME-Timing-ClearingTimeNs": "49344000.0000", "Core-Focus": "PIEZO", "528 AO-Block voltage": "false", "TE2000-Description": "Nikon TE2000 hub", "528 AO-MinVolts": "0.0000", "COM4-Parity": "None", "Summary": { "Slices": 11, "ChContrastMin": [ 0, 0, 0, 0, 0, 0 ], "PixelAspect": 1, "SlicesFirst": true, "Interval_ms": 0, "Time": "2019-12-18 23:10:50 -0500", "Prefix": "seq_5", "InitialPositionList": [ { "Label": "Pos0", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.21], "Focus": [17741.5], "XYStage": [ 44542.200000000004, 31636.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos1", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.22], "Focus": [17741.4], "XYStage": [ 43801.100000000006, 31637.9 ] }, "GridColumnIndex": 0 }, { "Label": "Pos2", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.5], "XYStage": [ 38561.3, 32040.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos3", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.185], "Focus": [17741.3], "XYStage": [ 37698.4, 32041.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos4", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.16], "Focus": [17741.5], "XYStage": [ 32375.4, 31758.9 ] }, "GridColumnIndex": 0 }, { "Label": "Pos5", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.17000000000002], "Focus": [17741.3], "XYStage": [ 31770.7, 31758.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos6", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.4], "XYStage": [ 26536.800000000003, 31068.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos7", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.205], "Focus": [17741.4], "XYStage": [ 25420.2, 31068.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos8", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.85], "Focus": [17741.3], "XYStage": [ 21059, 31508 ] }, "GridColumnIndex": 0 }, { "Label": "Pos9", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.82], "Focus": [17741.3], "XYStage": [ 19731.9, 31506.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos10", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.79], "Focus": [17741.4], "XYStage": [ 14528.5, 32007.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos11", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.83], "Focus": [17741.2], "XYStage": [ 13830.300000000001, 32009.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos12", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.79], "Focus": [17741.3], "XYStage": [ 8775.300000000001, 32017.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos13", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.815], "Focus": [17741.3], "XYStage": [ 8109.200000000001, 32008.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos14", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.805], "Focus": [17741.3], "XYStage": [ 3483.3, 31908.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos15", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.82], "Focus": [17741.2], "XYStage": [ 2686.8, 31903.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos16", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [211.48000000000002], "Focus": [17741.1], "XYStage": [ -1978.9, 31973.600000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos17", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [211.51500000000001], "Focus": [17741.1], "XYStage": [ -2957.8, 31982.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos18", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.195], "Focus": [17741.5], "XYStage": [ 47747.4, -576.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos19", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.23000000000002], "Focus": [17741.6], "XYStage": [ 47053.9, -576.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos20", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.205], "Focus": [17741.4], "XYStage": [ 41871.200000000004, -479.8 ] }, "GridColumnIndex": 0 }, { "Label": "Pos21", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.4], "XYStage": [ 40882.600000000006, -392 ] }, "GridColumnIndex": 0 }, { "Label": "Pos22", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.24], "Focus": [17741.4], "XYStage": [ 35905.6, -135.3 ] }, "GridColumnIndex": 0 }, { "Label": "Pos23", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.195], "Focus": [17741.5], "XYStage": [ 34990.9, -132.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos24", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.46], "Focus": [17741.4], "XYStage": [ 29678.100000000002, -958.1 ] }, "GridColumnIndex": 0 }, { "Label": "Pos25", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.44], "Focus": [17741.5], "XYStage": [ 28983.600000000002, -939.4000000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos26", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.475], "Focus": [17741.5], "XYStage": [ 23019.7, -122 ] }, "GridColumnIndex": 0 }, { "Label": "Pos27", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.44], "Focus": [17741.7], "XYStage": [ 22428, -116.80000000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos28", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.46], "Focus": [17741.7], "XYStage": [ 16364.900000000001, -181.10000000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos29", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.48000000000002], "Focus": [17741.5], "XYStage": [ 15712.1, -180 ] }, "GridColumnIndex": 0 }, { "Label": "Pos30", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.45000000000002], "Focus": [17741.7], "XYStage": [ 10346.1, 77.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos31", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.49], "Focus": [17741.4], "XYStage": [ 9755.5, 85.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos32", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.475], "Focus": [17741.5], "XYStage": [ 4775.5, 293.90000000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos33", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.445], "Focus": [17741.4], "XYStage": [ 3954.9, 293.90000000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos34", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.455], "Focus": [17741.4], "XYStage": [ -1324.9, 545.6 ] }, "GridColumnIndex": 0 }, { "Label": "Pos35", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.445], "Focus": [17741.3], "XYStage": [ -2097, 576.6 ] }, "GridColumnIndex": 0 } ], "z-step_um": 10, "GridRow": 0, "BitDepth": 16, "Depth": 2, "Channels": 6, "Source": "Micro-Manager", "ChColors": [ -16711681, -256, -65281, -1, -1, -16776961 ], "MetadataVersion": 10, "KeepShutterOpenChannels": true, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536, 65536 ], "CustomIntervals_ms": [], "Height": 1200, "MicroManagerVersion": "1.4.23 20180219", "GridColumn": 0, "UUID": "67598d21-5db5-4842-ab60-f506f7b42c7c", "PixelType": "GRAY16", "Width": 1200, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\Yan\\20191218-YC113-S7S8-miseq", "Frames": 1, "Comment": "", "UserName": "Nikon", "PixelSize_um": 1.1, "ROI": [ 0, 0, 1200, 1200 ], "Date": "2019-12-18", "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC", "P-DAPI-high" ], "KeepShutterOpenSlices": true, "IJType": 1, "ComputerName": "ZadorConfocal2", "Positions": 36, "PositionIndex": 29, "CameraTimeout": 20000, "TimeFirst": false }, "640 AO-Name": "AnalogIO", "Vincent-D3-Command": "Close" }, "FrameKey-0-1-3": { "COM4-StopBits": "1", "528 AO-MaxVolts": "5.0000", "XLIGHT Excitation Wheel-State": "1", "470 Shutter-Name": "AnalogIO", "Core-Shutter": "555", "555 AO-Demo": "No", "XLIGHT Dichroic-State": "2", "XYStage-StepSize-Y": "0.1000", "ZPositionUm": 198.48000000000002, "XYStage-StepSize-X": "0.1000", "PRIME-FirmwareVersion": "20.28", "PRIME-ClearCycles": "2", "520-Name": "DA Shutter", "405 Shutter-Name": "AnalogIO", "PIEZO-StepSize": "0.0050", "PRIME-ClearMode": "Pre-Exposure", "470 AO-MinVolts": "0.0000", "405 AO-SupportsTriggering": "1", "CameraChannelIndex": 0, "XYStage-StartSpeed": "500.0000", "COM9-StopBits": "2", "405 AO-Description": "NI DAC", "COM4-DelayBetweenCharsMs": "0.0000", "445 AO-Block voltage": "false", "520 AO-TriggerInputLine": "/Dev1/PFI0", "555 AO-Description": "NI DAC", "520 AO-TriggerSampleRate": "100000", "COM4-Verbose": "1", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM4-Name": "COM4", "405 Shutter-Volts": "0.0000", "Vincent-D3-Port": "COM1", "PRIME-PP 4 MIN ADU AFFECTED": "200", "470 Shutter-MinVolts": "0.0000", "PRIME-PP 3 ENABLED": "Yes", "470 AO-Description": "NI DAC", "PRIME-Description": "PVCAM API device adapter", "520 Shutter-MaxVolts": "5.0000", "520 Shutter-Volts": "0.0000", "528 AO-Sequenceable": "1", "PRIME-Binning": "1x1", "555 Shutter-MaxVolts": "5.0000", "528 Shutter-TriggerSequenceLength": "1000", "405 Shutter-Block voltage": "false", "XLIGHT Spin motor-State": "1", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "Vincent-D3-Time to open (ms)": "35.0000", "Vincent-D3-Time to close (ms)": "35.0000", "445 AO-TriggerSampleRate": "100000", "640 Shutter-IOChannel": "Dev1/ao14", "555 AO-Sequenceable": "1", "520 AO-Name": "AnalogIO", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "470 AO-Block voltage": "false", "COM1-Verbose": "1", "COM9-AnswerTimeout": "10000.0000", "528 Shutter-Name": "AnalogIO", "PRIME-Trigger Timeout (secs)": "10", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "NextFrame": 0, "470 AO-TriggerSequenceLength": "1000", "PRIME-TransposeXY": "0", "445 AO-TriggerSequenceLength": "1000", "COM1-Name": "COM1", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "COM9-DataBits": "8", "COM1-AnswerTimeout": "500.0000", "Camera": "", "TE2000-Version": "V2.50-V2.50-V2.10", "528 Shutter-Volts": "0.0000", "XLIGHT Hub-Name": "XLIGHT Hub", "PIEZO-Description": "Ludl stage driver adapter", "PositionName": "Pos29", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSampleRate": "100000", "PRIME-FanSpeedSetpoint": "High", "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "405 AO-TriggerSampleRate": "100000", "470 Shutter-Block voltage": "false", "470-State": "0", "COM3-Parity": "None", "405 AO-Volts": "0.0000", "PRIME-TransposeMirrorY": "0", "PRIME-ChipName": "GS144BSI", "470 AO-Volts": "0.0000", "PRIME-TransposeMirrorX": "0", "445 Shutter-SupportsTriggering": "0", "470 Shutter-SupportsTriggering": "0", "XLIGHT Hub-Description": "XLIGHT hub", "PRIME-PreampOffLimit": "10000", "470 AO-Sequenceable": "1", "528 Shutter-MaxVolts": "5.0000", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "Core-XYStage": "XYStage", "470 AO-TriggerSampleRate": "100000", "PRIME-Timing-PreTriggerDelayNs": "24672000.0000", "528-DA Device": "528 Shutter", "Focus-Description": "Nikon TE2000 focus adapter", "FilterBlock-State": "5", "520 AO-Demo": "No", "COM3-DelayBetweenCharsMs": "0.0000", "470 Shutter-Volts": "0.0000", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "PRIME-PP 2 THRESHOLD": "125", "PRIME-PP 4 THRESHOLD": "75", "XYStage-Acceleration": "4.0000", "470 Shutter-Demo": "No", "PRIME-Name": "PMPCIECam00", "640-DA Device": "640 Shutter", "405 AO-Block voltage": "false", "ElapsedTime-ms": 1198711, "PRIME-Offset": "100", "445 AO-IOChannel": "Dev1/ao1", "640 AO-MaxVolts": "5.0000", "PositionIndex": 29, "PRIME-PP 1 ENABLED": "Yes", "COM1-BaudRate": "9600", "640-State": "0", "COM3-Verbose": "1", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "LudlController-TransmissionDelay": "0", "PRIME-FullWellCapacity": "62000", "PRIME-SMARTStreamingEnabled": "No", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "640 Shutter-Description": "NI DAC", "528-Description": "DA device that is used as a shutter", "COM4-Description": "Serial port driver (boost:asio)", "405 Shutter-Description": "NI DAC", "TE2000-Name": "TE2000", "528 Shutter-Description": "NI DAC", "COM9-Description": "Serial port driver (boost:asio)", "LudlController-Name": "LudlController", "445 Shutter-TriggerSequenceLength": "1000", "PRIME-SerialNumber": "A18B203008", "445 AO-Volts": "0.0000", "PRIME-X-dimension": "1200", "PRIME-ShutterCloseDelay": "0", "XLIGHT Disk slider-State": "2", "Position": "Default", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 AO-IOChannel": "Dev1/ao5", "520 AO-IOChannel": "Dev1/ao4", "528 AO-TriggerSequenceLength": "1000", "405 Shutter-MaxVolts": "5.0000", "470-DA Device": "470 Shutter", "PRIME-FTCapable": "No", "Core-ChannelGroup": "Channels", "555 AO-SupportsTriggering": "0", "PRIME-Y-dimension": "1200", "Frame": 0, "555 Shutter-Sequenceable": "1", "PRIME-Timing-ExposureTimeNs": "499998000.0000", "Core-ImageProcessor": "", "528-State": "0", "520 AO-Block voltage": "false", "XLIGHT Excitation Wheel-Label": "QUAD", "640 Shutter-Demo": "No", "COM3-StopBits": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "520 Shutter-IOChannel": "Dev1/ao11", "Width": 1200, "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-SupportsTriggering": "0", "640 AO-TriggerSampleRate": "100000", "520-Description": "DA device that is used as a shutter", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "405 Shutter-Sequenceable": "1", "405 Shutter-SupportsTriggering": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-TriggerSampleRate": "100000", "640 Shutter-MaxVolts": "5.0000", "555 AO-MaxVolts": "5.0000", "520 AO-TriggerSequenceLength": "1000", "COM3-BaudRate": "9600", "COM9-BaudRate": "9600", "Vincent-D3-Channel #": "Ch. #1", "PRIME-PMode": "Normal", "OpticalPath-Label": "Left(100)", "BitDepth": 16, "555 AO-TriggerSequenceLength": "1000", "445 Shutter-Description": "NI DAC", "405 AO-Sequenceable": "1", "PRIME-AcquisitionMethod": "Callbacks", "555 AO-Name": "AnalogIO", "PRIME-ShutterMode": "Pre-Exposure", "COM3-Handshaking": "Off", "445 AO-SupportsTriggering": "0", "ROI": "0-0-1200-1200", "COM9-Verbose": "1", "PRIME-Color": "OFF", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "PRIME-ShutterOpenDelay": "0", "COM1-Parity": "None", "Slice": 3, "SlicePosition": -20, "COM9-DelayBetweenCharsMs": "0.0000", "555-Name": "DA Shutter", "PIEZO-LudlSingleAxisName": "B", "XLIGHT Emission Wheel-Label": "585/11(T)", "640 Shutter-Name": "AnalogIO", "640 AO-Sequenceable": "1", "520 Shutter-Demo": "No", "528 Shutter-Demo": "No", "470-Name": "DA Shutter", "520-DA Device": "520 Shutter", "XYStage-Description": "Ludl XY stage driver adapter", "445 Shutter-MinVolts": "0.0000", "528 AO-TriggerSampleRate": "100000", "405 AO-MinVolts": "0.0000", "405 Shutter-TriggerSequenceLength": "1000", "Height": 1200, "555 AO-Block voltage": "false", "640 Shutter-Volts": "0.0000", "PixelType": "GRAY16", "445 Shutter-Block voltage": "false", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "520 Shutter-TriggerSequenceLength": "1000", "COM9-Name": "COM9", "Core-SLM": "", "555 AO-Volts": "4.5000", "555 Shutter-Block voltage": "false", "XYStage-Speed": "1800.0000", "520 AO-MaxVolts": "5.0000", "528 Shutter-SupportsTriggering": "0", "COM1-Description": "Serial port driver (boost:asio)", "PRIME-MetadataEnabled": "No", "520 Shutter-MinVolts": "0.0000", "470 Shutter-Sequenceable": "1", "PRIME-PreampDelay": "0", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-MinVolts": "0.0000", "ChannelIndex": 1, "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Core-AutoShutter": "1", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "Exposure-ms": 160, "PRIME-PP 2 ENABLED": "Yes", "640 Shutter-TriggerSampleRate": "100000", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "470 Shutter-IOChannel": "Dev1/ao10", "405 AO-Demo": "No", "PixelSizeUm": 1.1, "470 AO-Demo": "No", "470 Shutter-MaxVolts": "5.0000", "Vincent-D3-Name": "Vincent-D3", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FileName": "img_000000000_Il-T_003.tif", "LudlController-Reset": "Operate", "PRIME-Exposure": "160.00", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "445-Name": "DA Shutter", "555 Shutter-Name": "AnalogIO", "520 Shutter-TriggerSampleRate": "100000", "445 Shutter-IOChannel": "Dev1/ao9", "PRIME-TransposeCorrection": "0", "XYStage-StepSize": "0.1000", "445-Description": "DA device that is used as a shutter", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 AO-IOChannel": "Dev1/ao7", "520 Shutter-Name": "AnalogIO", "COM3-DataBits": "8", "640 AO-Volts": "0.0000", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "555 Shutter-IOChannel": "Dev1/ao13", "WaitInterval": null, "520 Shutter-Sequenceable": "1", "YPositionUm": -180, "445-State": "0", "445-DA Device": "445 Shutter", "PRIME-CircularBufferAutoSize": "ON", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "555 AO-TriggerSampleRate": "100000", "445 Shutter-Volts": "0.0000", "XLIGHT Hub-Port": "COM3", "Core-AutoFocus": "", "COM4-Handshaking": "Off", "528 Shutter-MinVolts": "0.0000", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "445 Shutter-Name": "AnalogIO", "528 Shutter-Sequenceable": "1", "XYStage-TransposeMirrorX": "0", "XYStage-TransposeMirrorY": "0", "PRIME-ReadoutRate": "100MHz 16bit", "XYStage-Name": "Stage", "640 AO-SupportsTriggering": "0", "640-Name": "DA Shutter", "640 Shutter-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "640 AO-Block voltage": "false", "445 Shutter-Sequenceable": "1", "470 AO-MaxVolts": "5.0000", "COM4-BaudRate": "9600", "470-Description": "DA device that is used as a shutter", "520 Shutter-Description": "NI DAC", "PRIME-CCDTemperature": "-14.8500", "Time": "2019-12-18 23:10:58 -0500", "640 AO-TriggerSequenceLength": "1000", "470 AO-Name": "AnalogIO", "XLIGHT Disk slider-Label": "Disk Pos 40um", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "Binning": "1x1", "TE2000-ModelType": "1", "COM3-AnswerTimeout": "500.0000", "555 Shutter-SupportsTriggering": "0", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "TE2000-Port": "COM4", "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Description": "NI DAC", "COM4-AnswerTimeout": "500.0000", "COM1-DelayBetweenCharsMs": "0.0000", "FilterBlock-Label": "Confocal", "445 AO-Name": "AnalogIO", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-Timing-ReadoutTimeNs": "24672000.0000", "528 AO-Demo": "No", "520 AO-Sequenceable": "1", "555-Description": "DA device that is used as a shutter", "405-DA Device": "405 Shutter", "405 AO-TriggerSequenceLength": "1000", "640 AO-MinVolts": "0.0000", "640 Shutter-Block voltage": "false", "FrameIndex": 0, "PRIME-Port": "CMOS", "555 AO-MinVolts": "0.0000", "COM3-Description": "Serial port driver (boost:asio)", "528 AO-Description": "NI DAC", "555 AO-IOChannel": "Dev1/ao6", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "555 AO-TriggerInputLine": "/Dev1/PFI0", "PRIME-ActualInterval-ms": "390.5200", "LudlController-Port": "COM9", "445 Shutter-Demo": "No", "PRIME-Trigger-Expose Out-Mux": "1", "SliceIndex": 3, "PRIME-CCDTemperatureSetPoint": "-15.0000", "405 Shutter-MinVolts": "0.0000", "PRIME-ExposeOutMode": "First Row", "AxisPositions": null, "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Volts": "5.0000", "528-Name": "DA Shutter", "555-State": "1", "XLIGHT Dichroic-Label": "QUAD", "445 AO-Sequenceable": "1", "405-Name": "DA Shutter", "405 AO-IOChannel": "Dev1/ao0", "Core-Camera": "PRIME", "Focus-Name": "Focus", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "405 Shutter-IOChannel": "Dev1/ao8", "640 AO-Demo": "No", "640 Shutter-SupportsTriggering": "0", "445 AO-Demo": "No", "555-DA Device": "555 Shutter", "470 Shutter-Description": "NI DAC", "528 AO-Volts": "0.0000", "COM1-DataBits": "8", "Vincent-D3-Address": "x", "520 AO-SupportsTriggering": "0", "405-Description": "DA device that is used as a shutter", "640 Shutter-MinVolts": "0.0000", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "405 AO-Name": "AnalogIO", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "640 AO-TriggerInputLine": "/Dev1/PFI0", "405 AO-TriggerInputLine": "/Dev1/PFI0", "640-Description": "DA device that is used as a shutter", "445 Shutter-TriggerSampleRate": "100000", "470 Shutter-TriggerSequenceLength": "1000", "COM1-StopBits": "1", "520 Shutter-SupportsTriggering": "0", "405-State": "0", "Core-Initialize": "1", "XLIGHT Emission Wheel-State": "3", "520 AO-Description": "NI DAC", "528 AO-TriggerInputLine": "/Dev1/PFI0", "445 Shutter-MaxVolts": "5.0000", "COM9-Handshaking": "Off", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "XLIGHT Spin motor-Label": "On (spinning)", "PRIME-PP 5 ENABLED": "No", "UUID": "5120813d-0b9c-411d-8a37-63f97a34ac66", "PRIME-TriggerMode": "Internal Trigger", "COM1-Handshaking": "Off", "PRIME-Timing-PostTriggerDelayNs": "49344000.0000", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "Focus-Position": "17741.5000", "528 Shutter-Block voltage": "false", "Channel": "Il-T", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "555 Shutter-Demo": "No", "405 Shutter-Demo": "No", "528 AO-Name": "AnalogIO", "520-State": "0", "Core-Galvo": "", "405 Shutter-TriggerSampleRate": "100000", "Focus-FocusStepSize_(nm)": "100", "PRIME-PixelType": "16bit", "COM4-DataBits": "8", "470 AO-IOChannel": "Dev1/ao2", "520 AO-MinVolts": "0.0000", "COM9-Parity": "None", "PRIME-CircularBufferEnabled": "ON", "PRIME-PP 6 Reset": "No", "640 AO-Description": "NI DAC", "Source": "PRIME", "445 AO-MinVolts": "0.0000", "PIEZO-Name": "Stage", "445 AO-Description": "NI DAC", "PRIME-CircularBufferFrameCount": "46", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "520 AO-Volts": "0.0000", "528 Shutter-TriggerSampleRate": "100000", "XPositionUm": 15712.1, "OpticalPath-State": "4", "PRIME-PP 3 THRESHOLD": "80", "PRIME-PVCAM Version": "3.7.1", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Gain": "1-HDR", "640 Shutter-TriggerSequenceLength": "1000", "528 AO-SupportsTriggering": "0", "445 AO-TriggerInputLine": "/Dev1/PFI0", "OpticalPath-Name": "OpticalPath", "PRIME-PP 4 ENABLED": "Yes", "PRIME-PVCAM Adapter Version": "1.3.29", "COM3-Name": "COM3", "405 AO-MaxVolts": "5.0000", "520 Shutter-Block voltage": "false", "PRIME-Timing-ClearingTimeNs": "49344000.0000", "Core-Focus": "PIEZO", "528 AO-Block voltage": "false", "TE2000-Description": "Nikon TE2000 hub", "528 AO-MinVolts": "0.0000", "COM4-Parity": "None", "Summary": { "Slices": 11, "ChContrastMin": [ 0, 0, 0, 0, 0, 0 ], "PixelAspect": 1, "SlicesFirst": true, "Interval_ms": 0, "Time": "2019-12-18 23:10:50 -0500", "Prefix": "seq_5", "InitialPositionList": [ { "Label": "Pos0", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.21], "Focus": [17741.5], "XYStage": [ 44542.200000000004, 31636.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos1", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.22], "Focus": [17741.4], "XYStage": [ 43801.100000000006, 31637.9 ] }, "GridColumnIndex": 0 }, { "Label": "Pos2", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.5], "XYStage": [ 38561.3, 32040.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos3", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.185], "Focus": [17741.3], "XYStage": [ 37698.4, 32041.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos4", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.16], "Focus": [17741.5], "XYStage": [ 32375.4, 31758.9 ] }, "GridColumnIndex": 0 }, { "Label": "Pos5", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.17000000000002], "Focus": [17741.3], "XYStage": [ 31770.7, 31758.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos6", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.4], "XYStage": [ 26536.800000000003, 31068.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos7", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.205], "Focus": [17741.4], "XYStage": [ 25420.2, 31068.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos8", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.85], "Focus": [17741.3], "XYStage": [ 21059, 31508 ] }, "GridColumnIndex": 0 }, { "Label": "Pos9", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.82], "Focus": [17741.3], "XYStage": [ 19731.9, 31506.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos10", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.79], "Focus": [17741.4], "XYStage": [ 14528.5, 32007.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos11", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.83], "Focus": [17741.2], "XYStage": [ 13830.300000000001, 32009.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos12", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.79], "Focus": [17741.3], "XYStage": [ 8775.300000000001, 32017.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos13", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.815], "Focus": [17741.3], "XYStage": [ 8109.200000000001, 32008.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos14", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.805], "Focus": [17741.3], "XYStage": [ 3483.3, 31908.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos15", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.82], "Focus": [17741.2], "XYStage": [ 2686.8, 31903.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos16", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [211.48000000000002], "Focus": [17741.1], "XYStage": [ -1978.9, 31973.600000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos17", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [211.51500000000001], "Focus": [17741.1], "XYStage": [ -2957.8, 31982.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos18", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.195], "Focus": [17741.5], "XYStage": [ 47747.4, -576.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos19", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.23000000000002], "Focus": [17741.6], "XYStage": [ 47053.9, -576.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos20", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.205], "Focus": [17741.4], "XYStage": [ 41871.200000000004, -479.8 ] }, "GridColumnIndex": 0 }, { "Label": "Pos21", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.4], "XYStage": [ 40882.600000000006, -392 ] }, "GridColumnIndex": 0 }, { "Label": "Pos22", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.24], "Focus": [17741.4], "XYStage": [ 35905.6, -135.3 ] }, "GridColumnIndex": 0 }, { "Label": "Pos23", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.195], "Focus": [17741.5], "XYStage": [ 34990.9, -132.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos24", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.46], "Focus": [17741.4], "XYStage": [ 29678.100000000002, -958.1 ] }, "GridColumnIndex": 0 }, { "Label": "Pos25", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.44], "Focus": [17741.5], "XYStage": [ 28983.600000000002, -939.4000000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos26", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.475], "Focus": [17741.5], "XYStage": [ 23019.7, -122 ] }, "GridColumnIndex": 0 }, { "Label": "Pos27", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.44], "Focus": [17741.7], "XYStage": [ 22428, -116.80000000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos28", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.46], "Focus": [17741.7], "XYStage": [ 16364.900000000001, -181.10000000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos29", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.48000000000002], "Focus": [17741.5], "XYStage": [ 15712.1, -180 ] }, "GridColumnIndex": 0 }, { "Label": "Pos30", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.45000000000002], "Focus": [17741.7], "XYStage": [ 10346.1, 77.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos31", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.49], "Focus": [17741.4], "XYStage": [ 9755.5, 85.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos32", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.475], "Focus": [17741.5], "XYStage": [ 4775.5, 293.90000000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos33", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.445], "Focus": [17741.4], "XYStage": [ 3954.9, 293.90000000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos34", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.455], "Focus": [17741.4], "XYStage": [ -1324.9, 545.6 ] }, "GridColumnIndex": 0 }, { "Label": "Pos35", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.445], "Focus": [17741.3], "XYStage": [ -2097, 576.6 ] }, "GridColumnIndex": 0 } ], "z-step_um": 10, "GridRow": 0, "BitDepth": 16, "Depth": 2, "Channels": 6, "Source": "Micro-Manager", "ChColors": [ -16711681, -256, -65281, -1, -1, -16776961 ], "MetadataVersion": 10, "KeepShutterOpenChannels": true, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536, 65536 ], "CustomIntervals_ms": [], "Height": 1200, "MicroManagerVersion": "1.4.23 20180219", "GridColumn": 0, "UUID": "67598d21-5db5-4842-ab60-f506f7b42c7c", "PixelType": "GRAY16", "Width": 1200, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\Yan\\20191218-YC113-S7S8-miseq", "Frames": 1, "Comment": "", "UserName": "Nikon", "PixelSize_um": 1.1, "ROI": [ 0, 0, 1200, 1200 ], "Date": "2019-12-18", "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC", "P-DAPI-high" ], "KeepShutterOpenSlices": true, "IJType": 1, "ComputerName": "ZadorConfocal2", "Positions": 36, "PositionIndex": 29, "CameraTimeout": 20000, "TimeFirst": false }, "640 AO-Name": "AnalogIO", "Vincent-D3-Command": "Close" }, "FrameKey-0-1-4": { "COM4-StopBits": "1", "528 AO-MaxVolts": "5.0000", "XLIGHT Excitation Wheel-State": "1", "470 Shutter-Name": "AnalogIO", "Core-Shutter": "555", "555 AO-Demo": "No", "XLIGHT Dichroic-State": "2", "XYStage-StepSize-Y": "0.1000", "ZPositionUm": 208.48000000000002, "XYStage-StepSize-X": "0.1000", "PRIME-FirmwareVersion": "20.28", "PRIME-ClearCycles": "2", "520-Name": "DA Shutter", "405 Shutter-Name": "AnalogIO", "PIEZO-StepSize": "0.0050", "PRIME-ClearMode": "Pre-Exposure", "470 AO-MinVolts": "0.0000", "405 AO-SupportsTriggering": "1", "CameraChannelIndex": 0, "XYStage-StartSpeed": "500.0000", "COM9-StopBits": "2", "405 AO-Description": "NI DAC", "COM4-DelayBetweenCharsMs": "0.0000", "445 AO-Block voltage": "false", "520 AO-TriggerInputLine": "/Dev1/PFI0", "555 AO-Description": "NI DAC", "520 AO-TriggerSampleRate": "100000", "COM4-Verbose": "1", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM4-Name": "COM4", "405 Shutter-Volts": "0.0000", "Vincent-D3-Port": "COM1", "PRIME-PP 4 MIN ADU AFFECTED": "200", "470 Shutter-MinVolts": "0.0000", "PRIME-PP 3 ENABLED": "Yes", "470 AO-Description": "NI DAC", "PRIME-Description": "PVCAM API device adapter", "520 Shutter-MaxVolts": "5.0000", "520 Shutter-Volts": "0.0000", "528 AO-Sequenceable": "1", "PRIME-Binning": "1x1", "555 Shutter-MaxVolts": "5.0000", "528 Shutter-TriggerSequenceLength": "1000", "405 Shutter-Block voltage": "false", "XLIGHT Spin motor-State": "1", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "Vincent-D3-Time to open (ms)": "35.0000", "Vincent-D3-Time to close (ms)": "35.0000", "445 AO-TriggerSampleRate": "100000", "640 Shutter-IOChannel": "Dev1/ao14", "555 AO-Sequenceable": "1", "520 AO-Name": "AnalogIO", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "470 AO-Block voltage": "false", "COM1-Verbose": "1", "COM9-AnswerTimeout": "10000.0000", "528 Shutter-Name": "AnalogIO", "PRIME-Trigger Timeout (secs)": "10", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "NextFrame": 0, "470 AO-TriggerSequenceLength": "1000", "PRIME-TransposeXY": "0", "445 AO-TriggerSequenceLength": "1000", "COM1-Name": "COM1", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "COM9-DataBits": "8", "COM1-AnswerTimeout": "500.0000", "Camera": "", "TE2000-Version": "V2.50-V2.50-V2.10", "528 Shutter-Volts": "0.0000", "XLIGHT Hub-Name": "XLIGHT Hub", "PIEZO-Description": "Ludl stage driver adapter", "PositionName": "Pos29", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSampleRate": "100000", "PRIME-FanSpeedSetpoint": "High", "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "405 AO-TriggerSampleRate": "100000", "470 Shutter-Block voltage": "false", "470-State": "0", "COM3-Parity": "None", "405 AO-Volts": "0.0000", "PRIME-TransposeMirrorY": "0", "PRIME-ChipName": "GS144BSI", "470 AO-Volts": "0.0000", "PRIME-TransposeMirrorX": "0", "445 Shutter-SupportsTriggering": "0", "470 Shutter-SupportsTriggering": "0", "XLIGHT Hub-Description": "XLIGHT hub", "PRIME-PreampOffLimit": "10000", "470 AO-Sequenceable": "1", "528 Shutter-MaxVolts": "5.0000", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "Core-XYStage": "XYStage", "470 AO-TriggerSampleRate": "100000", "PRIME-Timing-PreTriggerDelayNs": "24672000.0000", "528-DA Device": "528 Shutter", "Focus-Description": "Nikon TE2000 focus adapter", "FilterBlock-State": "5", "520 AO-Demo": "No", "COM3-DelayBetweenCharsMs": "0.0000", "470 Shutter-Volts": "0.0000", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "PRIME-PP 2 THRESHOLD": "125", "PRIME-PP 4 THRESHOLD": "75", "XYStage-Acceleration": "4.0000", "470 Shutter-Demo": "No", "PRIME-Name": "PMPCIECam00", "640-DA Device": "640 Shutter", "405 AO-Block voltage": "false", "ElapsedTime-ms": 1199109, "PRIME-Offset": "100", "445 AO-IOChannel": "Dev1/ao1", "640 AO-MaxVolts": "5.0000", "PositionIndex": 29, "PRIME-PP 1 ENABLED": "Yes", "COM1-BaudRate": "9600", "640-State": "0", "COM3-Verbose": "1", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "LudlController-TransmissionDelay": "0", "PRIME-FullWellCapacity": "62000", "PRIME-SMARTStreamingEnabled": "No", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "640 Shutter-Description": "NI DAC", "528-Description": "DA device that is used as a shutter", "COM4-Description": "Serial port driver (boost:asio)", "405 Shutter-Description": "NI DAC", "TE2000-Name": "TE2000", "528 Shutter-Description": "NI DAC", "COM9-Description": "Serial port driver (boost:asio)", "LudlController-Name": "LudlController", "445 Shutter-TriggerSequenceLength": "1000", "PRIME-SerialNumber": "A18B203008", "445 AO-Volts": "0.0000", "PRIME-X-dimension": "1200", "PRIME-ShutterCloseDelay": "0", "XLIGHT Disk slider-State": "2", "Position": "Default", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 AO-IOChannel": "Dev1/ao5", "520 AO-IOChannel": "Dev1/ao4", "528 AO-TriggerSequenceLength": "1000", "405 Shutter-MaxVolts": "5.0000", "470-DA Device": "470 Shutter", "PRIME-FTCapable": "No", "Core-ChannelGroup": "Channels", "555 AO-SupportsTriggering": "0", "PRIME-Y-dimension": "1200", "Frame": 0, "555 Shutter-Sequenceable": "1", "PRIME-Timing-ExposureTimeNs": "499998000.0000", "Core-ImageProcessor": "", "528-State": "0", "520 AO-Block voltage": "false", "XLIGHT Excitation Wheel-Label": "QUAD", "640 Shutter-Demo": "No", "COM3-StopBits": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "520 Shutter-IOChannel": "Dev1/ao11", "Width": 1200, "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-SupportsTriggering": "0", "640 AO-TriggerSampleRate": "100000", "520-Description": "DA device that is used as a shutter", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "405 Shutter-Sequenceable": "1", "405 Shutter-SupportsTriggering": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-TriggerSampleRate": "100000", "640 Shutter-MaxVolts": "5.0000", "555 AO-MaxVolts": "5.0000", "520 AO-TriggerSequenceLength": "1000", "COM3-BaudRate": "9600", "COM9-BaudRate": "9600", "Vincent-D3-Channel #": "Ch. #1", "PRIME-PMode": "Normal", "OpticalPath-Label": "Left(100)", "BitDepth": 16, "555 AO-TriggerSequenceLength": "1000", "445 Shutter-Description": "NI DAC", "405 AO-Sequenceable": "1", "PRIME-AcquisitionMethod": "Callbacks", "555 AO-Name": "AnalogIO", "PRIME-ShutterMode": "Pre-Exposure", "COM3-Handshaking": "Off", "445 AO-SupportsTriggering": "0", "ROI": "0-0-1200-1200", "COM9-Verbose": "1", "PRIME-Color": "OFF", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "PRIME-ShutterOpenDelay": "0", "COM1-Parity": "None", "Slice": 4, "SlicePosition": -10, "COM9-DelayBetweenCharsMs": "0.0000", "555-Name": "DA Shutter", "PIEZO-LudlSingleAxisName": "B", "XLIGHT Emission Wheel-Label": "585/11(T)", "640 Shutter-Name": "AnalogIO", "640 AO-Sequenceable": "1", "520 Shutter-Demo": "No", "528 Shutter-Demo": "No", "470-Name": "DA Shutter", "520-DA Device": "520 Shutter", "XYStage-Description": "Ludl XY stage driver adapter", "445 Shutter-MinVolts": "0.0000", "528 AO-TriggerSampleRate": "100000", "405 AO-MinVolts": "0.0000", "405 Shutter-TriggerSequenceLength": "1000", "Height": 1200, "555 AO-Block voltage": "false", "640 Shutter-Volts": "0.0000", "PixelType": "GRAY16", "445 Shutter-Block voltage": "false", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "520 Shutter-TriggerSequenceLength": "1000", "COM9-Name": "COM9", "Core-SLM": "", "555 AO-Volts": "4.5000", "555 Shutter-Block voltage": "false", "XYStage-Speed": "1800.0000", "520 AO-MaxVolts": "5.0000", "528 Shutter-SupportsTriggering": "0", "COM1-Description": "Serial port driver (boost:asio)", "PRIME-MetadataEnabled": "No", "520 Shutter-MinVolts": "0.0000", "470 Shutter-Sequenceable": "1", "PRIME-PreampDelay": "0", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-MinVolts": "0.0000", "ChannelIndex": 1, "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Core-AutoShutter": "1", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "Exposure-ms": 160, "PRIME-PP 2 ENABLED": "Yes", "640 Shutter-TriggerSampleRate": "100000", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "470 Shutter-IOChannel": "Dev1/ao10", "405 AO-Demo": "No", "PixelSizeUm": 1.1, "470 AO-Demo": "No", "470 Shutter-MaxVolts": "5.0000", "Vincent-D3-Name": "Vincent-D3", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FileName": "img_000000000_Il-T_004.tif", "LudlController-Reset": "Operate", "PRIME-Exposure": "160.00", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "445-Name": "DA Shutter", "555 Shutter-Name": "AnalogIO", "520 Shutter-TriggerSampleRate": "100000", "445 Shutter-IOChannel": "Dev1/ao9", "PRIME-TransposeCorrection": "0", "XYStage-StepSize": "0.1000", "445-Description": "DA device that is used as a shutter", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 AO-IOChannel": "Dev1/ao7", "520 Shutter-Name": "AnalogIO", "COM3-DataBits": "8", "640 AO-Volts": "0.0000", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "555 Shutter-IOChannel": "Dev1/ao13", "WaitInterval": null, "520 Shutter-Sequenceable": "1", "YPositionUm": -180, "445-State": "0", "445-DA Device": "445 Shutter", "PRIME-CircularBufferAutoSize": "ON", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "555 AO-TriggerSampleRate": "100000", "445 Shutter-Volts": "0.0000", "XLIGHT Hub-Port": "COM3", "Core-AutoFocus": "", "COM4-Handshaking": "Off", "528 Shutter-MinVolts": "0.0000", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "445 Shutter-Name": "AnalogIO", "528 Shutter-Sequenceable": "1", "XYStage-TransposeMirrorX": "0", "XYStage-TransposeMirrorY": "0", "PRIME-ReadoutRate": "100MHz 16bit", "XYStage-Name": "Stage", "640 AO-SupportsTriggering": "0", "640-Name": "DA Shutter", "640 Shutter-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "640 AO-Block voltage": "false", "445 Shutter-Sequenceable": "1", "470 AO-MaxVolts": "5.0000", "COM4-BaudRate": "9600", "470-Description": "DA device that is used as a shutter", "520 Shutter-Description": "NI DAC", "PRIME-CCDTemperature": "-14.8500", "Time": "2019-12-18 23:10:59 -0500", "640 AO-TriggerSequenceLength": "1000", "470 AO-Name": "AnalogIO", "XLIGHT Disk slider-Label": "Disk Pos 40um", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "Binning": "1x1", "TE2000-ModelType": "1", "COM3-AnswerTimeout": "500.0000", "555 Shutter-SupportsTriggering": "0", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "TE2000-Port": "COM4", "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Description": "NI DAC", "COM4-AnswerTimeout": "500.0000", "COM1-DelayBetweenCharsMs": "0.0000", "FilterBlock-Label": "Confocal", "445 AO-Name": "AnalogIO", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-Timing-ReadoutTimeNs": "24672000.0000", "528 AO-Demo": "No", "520 AO-Sequenceable": "1", "555-Description": "DA device that is used as a shutter", "405-DA Device": "405 Shutter", "405 AO-TriggerSequenceLength": "1000", "640 AO-MinVolts": "0.0000", "640 Shutter-Block voltage": "false", "FrameIndex": 0, "PRIME-Port": "CMOS", "555 AO-MinVolts": "0.0000", "COM3-Description": "Serial port driver (boost:asio)", "528 AO-Description": "NI DAC", "555 AO-IOChannel": "Dev1/ao6", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "555 AO-TriggerInputLine": "/Dev1/PFI0", "PRIME-ActualInterval-ms": "390.5200", "LudlController-Port": "COM9", "445 Shutter-Demo": "No", "PRIME-Trigger-Expose Out-Mux": "1", "SliceIndex": 4, "PRIME-CCDTemperatureSetPoint": "-15.0000", "405 Shutter-MinVolts": "0.0000", "PRIME-ExposeOutMode": "First Row", "AxisPositions": null, "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Volts": "5.0000", "528-Name": "DA Shutter", "555-State": "1", "XLIGHT Dichroic-Label": "QUAD", "445 AO-Sequenceable": "1", "405-Name": "DA Shutter", "405 AO-IOChannel": "Dev1/ao0", "Core-Camera": "PRIME", "Focus-Name": "Focus", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "405 Shutter-IOChannel": "Dev1/ao8", "640 AO-Demo": "No", "640 Shutter-SupportsTriggering": "0", "445 AO-Demo": "No", "555-DA Device": "555 Shutter", "470 Shutter-Description": "NI DAC", "528 AO-Volts": "0.0000", "COM1-DataBits": "8", "Vincent-D3-Address": "x", "520 AO-SupportsTriggering": "0", "405-Description": "DA device that is used as a shutter", "640 Shutter-MinVolts": "0.0000", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "405 AO-Name": "AnalogIO", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "640 AO-TriggerInputLine": "/Dev1/PFI0", "405 AO-TriggerInputLine": "/Dev1/PFI0", "640-Description": "DA device that is used as a shutter", "445 Shutter-TriggerSampleRate": "100000", "470 Shutter-TriggerSequenceLength": "1000", "COM1-StopBits": "1", "520 Shutter-SupportsTriggering": "0", "405-State": "0", "Core-Initialize": "1", "XLIGHT Emission Wheel-State": "3", "520 AO-Description": "NI DAC", "528 AO-TriggerInputLine": "/Dev1/PFI0", "445 Shutter-MaxVolts": "5.0000", "COM9-Handshaking": "Off", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "XLIGHT Spin motor-Label": "On (spinning)", "PRIME-PP 5 ENABLED": "No", "UUID": "c7d7e7bf-baf0-4e26-b464-3b31c5e8b516", "PRIME-TriggerMode": "Internal Trigger", "COM1-Handshaking": "Off", "PRIME-Timing-PostTriggerDelayNs": "49344000.0000", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "Focus-Position": "17741.5000", "528 Shutter-Block voltage": "false", "Channel": "Il-T", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "555 Shutter-Demo": "No", "405 Shutter-Demo": "No", "528 AO-Name": "AnalogIO", "520-State": "0", "Core-Galvo": "", "405 Shutter-TriggerSampleRate": "100000", "Focus-FocusStepSize_(nm)": "100", "PRIME-PixelType": "16bit", "COM4-DataBits": "8", "470 AO-IOChannel": "Dev1/ao2", "520 AO-MinVolts": "0.0000", "COM9-Parity": "None", "PRIME-CircularBufferEnabled": "ON", "PRIME-PP 6 Reset": "No", "640 AO-Description": "NI DAC", "Source": "PRIME", "445 AO-MinVolts": "0.0000", "PIEZO-Name": "Stage", "445 AO-Description": "NI DAC", "PRIME-CircularBufferFrameCount": "46", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "520 AO-Volts": "0.0000", "528 Shutter-TriggerSampleRate": "100000", "XPositionUm": 15712.1, "OpticalPath-State": "4", "PRIME-PP 3 THRESHOLD": "80", "PRIME-PVCAM Version": "3.7.1", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Gain": "1-HDR", "640 Shutter-TriggerSequenceLength": "1000", "528 AO-SupportsTriggering": "0", "445 AO-TriggerInputLine": "/Dev1/PFI0", "OpticalPath-Name": "OpticalPath", "PRIME-PP 4 ENABLED": "Yes", "PRIME-PVCAM Adapter Version": "1.3.29", "COM3-Name": "COM3", "405 AO-MaxVolts": "5.0000", "520 Shutter-Block voltage": "false", "PRIME-Timing-ClearingTimeNs": "49344000.0000", "Core-Focus": "PIEZO", "528 AO-Block voltage": "false", "TE2000-Description": "Nikon TE2000 hub", "528 AO-MinVolts": "0.0000", "COM4-Parity": "None", "Summary": { "Slices": 11, "ChContrastMin": [ 0, 0, 0, 0, 0, 0 ], "PixelAspect": 1, "SlicesFirst": true, "Interval_ms": 0, "Time": "2019-12-18 23:10:50 -0500", "Prefix": "seq_5", "InitialPositionList": [ { "Label": "Pos0", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.21], "Focus": [17741.5], "XYStage": [ 44542.200000000004, 31636.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos1", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.22], "Focus": [17741.4], "XYStage": [ 43801.100000000006, 31637.9 ] }, "GridColumnIndex": 0 }, { "Label": "Pos2", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.5], "XYStage": [ 38561.3, 32040.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos3", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.185], "Focus": [17741.3], "XYStage": [ 37698.4, 32041.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos4", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.16], "Focus": [17741.5], "XYStage": [ 32375.4, 31758.9 ] }, "GridColumnIndex": 0 }, { "Label": "Pos5", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.17000000000002], "Focus": [17741.3], "XYStage": [ 31770.7, 31758.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos6", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.4], "XYStage": [ 26536.800000000003, 31068.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos7", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.205], "Focus": [17741.4], "XYStage": [ 25420.2, 31068.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos8", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.85], "Focus": [17741.3], "XYStage": [ 21059, 31508 ] }, "GridColumnIndex": 0 }, { "Label": "Pos9", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.82], "Focus": [17741.3], "XYStage": [ 19731.9, 31506.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos10", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.79], "Focus": [17741.4], "XYStage": [ 14528.5, 32007.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos11", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.83], "Focus": [17741.2], "XYStage": [ 13830.300000000001, 32009.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos12", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.79], "Focus": [17741.3], "XYStage": [ 8775.300000000001, 32017.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos13", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.815], "Focus": [17741.3], "XYStage": [ 8109.200000000001, 32008.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos14", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.805], "Focus": [17741.3], "XYStage": [ 3483.3, 31908.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos15", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.82], "Focus": [17741.2], "XYStage": [ 2686.8, 31903.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos16", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [211.48000000000002], "Focus": [17741.1], "XYStage": [ -1978.9, 31973.600000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos17", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [211.51500000000001], "Focus": [17741.1], "XYStage": [ -2957.8, 31982.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos18", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.195], "Focus": [17741.5], "XYStage": [ 47747.4, -576.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos19", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.23000000000002], "Focus": [17741.6], "XYStage": [ 47053.9, -576.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos20", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.205], "Focus": [17741.4], "XYStage": [ 41871.200000000004, -479.8 ] }, "GridColumnIndex": 0 }, { "Label": "Pos21", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.4], "XYStage": [ 40882.600000000006, -392 ] }, "GridColumnIndex": 0 }, { "Label": "Pos22", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.24], "Focus": [17741.4], "XYStage": [ 35905.6, -135.3 ] }, "GridColumnIndex": 0 }, { "Label": "Pos23", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.195], "Focus": [17741.5], "XYStage": [ 34990.9, -132.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos24", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.46], "Focus": [17741.4], "XYStage": [ 29678.100000000002, -958.1 ] }, "GridColumnIndex": 0 }, { "Label": "Pos25", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.44], "Focus": [17741.5], "XYStage": [ 28983.600000000002, -939.4000000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos26", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.475], "Focus": [17741.5], "XYStage": [ 23019.7, -122 ] }, "GridColumnIndex": 0 }, { "Label": "Pos27", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.44], "Focus": [17741.7], "XYStage": [ 22428, -116.80000000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos28", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.46], "Focus": [17741.7], "XYStage": [ 16364.900000000001, -181.10000000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos29", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.48000000000002], "Focus": [17741.5], "XYStage": [ 15712.1, -180 ] }, "GridColumnIndex": 0 }, { "Label": "Pos30", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.45000000000002], "Focus": [17741.7], "XYStage": [ 10346.1, 77.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos31", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.49], "Focus": [17741.4], "XYStage": [ 9755.5, 85.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos32", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.475], "Focus": [17741.5], "XYStage": [ 4775.5, 293.90000000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos33", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.445], "Focus": [17741.4], "XYStage": [ 3954.9, 293.90000000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos34", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.455], "Focus": [17741.4], "XYStage": [ -1324.9, 545.6 ] }, "GridColumnIndex": 0 }, { "Label": "Pos35", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.445], "Focus": [17741.3], "XYStage": [ -2097, 576.6 ] }, "GridColumnIndex": 0 } ], "z-step_um": 10, "GridRow": 0, "BitDepth": 16, "Depth": 2, "Channels": 6, "Source": "Micro-Manager", "ChColors": [ -16711681, -256, -65281, -1, -1, -16776961 ], "MetadataVersion": 10, "KeepShutterOpenChannels": true, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536, 65536 ], "CustomIntervals_ms": [], "Height": 1200, "MicroManagerVersion": "1.4.23 20180219", "GridColumn": 0, "UUID": "67598d21-5db5-4842-ab60-f506f7b42c7c", "PixelType": "GRAY16", "Width": 1200, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\Yan\\20191218-YC113-S7S8-miseq", "Frames": 1, "Comment": "", "UserName": "Nikon", "PixelSize_um": 1.1, "ROI": [ 0, 0, 1200, 1200 ], "Date": "2019-12-18", "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC", "P-DAPI-high" ], "KeepShutterOpenSlices": true, "IJType": 1, "ComputerName": "ZadorConfocal2", "Positions": 36, "PositionIndex": 29, "CameraTimeout": 20000, "TimeFirst": false }, "640 AO-Name": "AnalogIO", "Vincent-D3-Command": "Close" }, "FrameKey-0-1-5": { "COM4-StopBits": "1", "528 AO-MaxVolts": "5.0000", "XLIGHT Excitation Wheel-State": "1", "470 Shutter-Name": "AnalogIO", "Core-Shutter": "555", "555 AO-Demo": "No", "XLIGHT Dichroic-State": "2", "XYStage-StepSize-Y": "0.1000", "ZPositionUm": 218.48000000000002, "XYStage-StepSize-X": "0.1000", "PRIME-FirmwareVersion": "20.28", "PRIME-ClearCycles": "2", "520-Name": "DA Shutter", "405 Shutter-Name": "AnalogIO", "PIEZO-StepSize": "0.0050", "PRIME-ClearMode": "Pre-Exposure", "470 AO-MinVolts": "0.0000", "405 AO-SupportsTriggering": "1", "CameraChannelIndex": 0, "XYStage-StartSpeed": "500.0000", "COM9-StopBits": "2", "405 AO-Description": "NI DAC", "COM4-DelayBetweenCharsMs": "0.0000", "445 AO-Block voltage": "false", "520 AO-TriggerInputLine": "/Dev1/PFI0", "555 AO-Description": "NI DAC", "520 AO-TriggerSampleRate": "100000", "COM4-Verbose": "1", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM4-Name": "COM4", "405 Shutter-Volts": "0.0000", "Vincent-D3-Port": "COM1", "PRIME-PP 4 MIN ADU AFFECTED": "200", "470 Shutter-MinVolts": "0.0000", "PRIME-PP 3 ENABLED": "Yes", "470 AO-Description": "NI DAC", "PRIME-Description": "PVCAM API device adapter", "520 Shutter-MaxVolts": "5.0000", "520 Shutter-Volts": "0.0000", "528 AO-Sequenceable": "1", "PRIME-Binning": "1x1", "555 Shutter-MaxVolts": "5.0000", "528 Shutter-TriggerSequenceLength": "1000", "405 Shutter-Block voltage": "false", "XLIGHT Spin motor-State": "1", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "Vincent-D3-Time to open (ms)": "35.0000", "Vincent-D3-Time to close (ms)": "35.0000", "445 AO-TriggerSampleRate": "100000", "640 Shutter-IOChannel": "Dev1/ao14", "555 AO-Sequenceable": "1", "520 AO-Name": "AnalogIO", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "470 AO-Block voltage": "false", "COM1-Verbose": "1", "COM9-AnswerTimeout": "10000.0000", "528 Shutter-Name": "AnalogIO", "PRIME-Trigger Timeout (secs)": "10", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "NextFrame": 0, "470 AO-TriggerSequenceLength": "1000", "PRIME-TransposeXY": "0", "445 AO-TriggerSequenceLength": "1000", "COM1-Name": "COM1", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "COM9-DataBits": "8", "COM1-AnswerTimeout": "500.0000", "Camera": "", "TE2000-Version": "V2.50-V2.50-V2.10", "528 Shutter-Volts": "0.0000", "XLIGHT Hub-Name": "XLIGHT Hub", "PIEZO-Description": "Ludl stage driver adapter", "PositionName": "Pos29", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSampleRate": "100000", "PRIME-FanSpeedSetpoint": "High", "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "405 AO-TriggerSampleRate": "100000", "470 Shutter-Block voltage": "false", "470-State": "0", "COM3-Parity": "None", "405 AO-Volts": "0.0000", "PRIME-TransposeMirrorY": "0", "PRIME-ChipName": "GS144BSI", "470 AO-Volts": "0.0000", "PRIME-TransposeMirrorX": "0", "445 Shutter-SupportsTriggering": "0", "470 Shutter-SupportsTriggering": "0", "XLIGHT Hub-Description": "XLIGHT hub", "PRIME-PreampOffLimit": "10000", "470 AO-Sequenceable": "1", "528 Shutter-MaxVolts": "5.0000", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "Core-XYStage": "XYStage", "470 AO-TriggerSampleRate": "100000", "PRIME-Timing-PreTriggerDelayNs": "24672000.0000", "528-DA Device": "528 Shutter", "Focus-Description": "Nikon TE2000 focus adapter", "FilterBlock-State": "5", "520 AO-Demo": "No", "COM3-DelayBetweenCharsMs": "0.0000", "470 Shutter-Volts": "0.0000", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "PRIME-PP 2 THRESHOLD": "125", "PRIME-PP 4 THRESHOLD": "75", "XYStage-Acceleration": "4.0000", "470 Shutter-Demo": "No", "PRIME-Name": "PMPCIECam00", "640-DA Device": "640 Shutter", "405 AO-Block voltage": "false", "ElapsedTime-ms": 1199507, "PRIME-Offset": "100", "445 AO-IOChannel": "Dev1/ao1", "640 AO-MaxVolts": "5.0000", "PositionIndex": 29, "PRIME-PP 1 ENABLED": "Yes", "COM1-BaudRate": "9600", "640-State": "0", "COM3-Verbose": "1", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "LudlController-TransmissionDelay": "0", "PRIME-FullWellCapacity": "62000", "PRIME-SMARTStreamingEnabled": "No", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "640 Shutter-Description": "NI DAC", "528-Description": "DA device that is used as a shutter", "COM4-Description": "Serial port driver (boost:asio)", "405 Shutter-Description": "NI DAC", "TE2000-Name": "TE2000", "528 Shutter-Description": "NI DAC", "COM9-Description": "Serial port driver (boost:asio)", "LudlController-Name": "LudlController", "445 Shutter-TriggerSequenceLength": "1000", "PRIME-SerialNumber": "A18B203008", "445 AO-Volts": "0.0000", "PRIME-X-dimension": "1200", "PRIME-ShutterCloseDelay": "0", "XLIGHT Disk slider-State": "2", "Position": "Default", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 AO-IOChannel": "Dev1/ao5", "520 AO-IOChannel": "Dev1/ao4", "528 AO-TriggerSequenceLength": "1000", "405 Shutter-MaxVolts": "5.0000", "470-DA Device": "470 Shutter", "PRIME-FTCapable": "No", "Core-ChannelGroup": "Channels", "555 AO-SupportsTriggering": "0", "PRIME-Y-dimension": "1200", "Frame": 0, "555 Shutter-Sequenceable": "1", "PRIME-Timing-ExposureTimeNs": "499998000.0000", "Core-ImageProcessor": "", "528-State": "0", "520 AO-Block voltage": "false", "XLIGHT Excitation Wheel-Label": "QUAD", "640 Shutter-Demo": "No", "COM3-StopBits": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "520 Shutter-IOChannel": "Dev1/ao11", "Width": 1200, "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-SupportsTriggering": "0", "640 AO-TriggerSampleRate": "100000", "520-Description": "DA device that is used as a shutter", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "405 Shutter-Sequenceable": "1", "405 Shutter-SupportsTriggering": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-TriggerSampleRate": "100000", "640 Shutter-MaxVolts": "5.0000", "555 AO-MaxVolts": "5.0000", "520 AO-TriggerSequenceLength": "1000", "COM3-BaudRate": "9600", "COM9-BaudRate": "9600", "Vincent-D3-Channel #": "Ch. #1", "PRIME-PMode": "Normal", "OpticalPath-Label": "Left(100)", "BitDepth": 16, "555 AO-TriggerSequenceLength": "1000", "445 Shutter-Description": "NI DAC", "405 AO-Sequenceable": "1", "PRIME-AcquisitionMethod": "Callbacks", "555 AO-Name": "AnalogIO", "PRIME-ShutterMode": "Pre-Exposure", "COM3-Handshaking": "Off", "445 AO-SupportsTriggering": "0", "ROI": "0-0-1200-1200", "COM9-Verbose": "1", "PRIME-Color": "OFF", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "PRIME-ShutterOpenDelay": "0", "COM1-Parity": "None", "Slice": 5, "SlicePosition": 0, "COM9-DelayBetweenCharsMs": "0.0000", "555-Name": "DA Shutter", "PIEZO-LudlSingleAxisName": "B", "XLIGHT Emission Wheel-Label": "585/11(T)", "640 Shutter-Name": "AnalogIO", "640 AO-Sequenceable": "1", "520 Shutter-Demo": "No", "528 Shutter-Demo": "No", "470-Name": "DA Shutter", "520-DA Device": "520 Shutter", "XYStage-Description": "Ludl XY stage driver adapter", "445 Shutter-MinVolts": "0.0000", "528 AO-TriggerSampleRate": "100000", "405 AO-MinVolts": "0.0000", "405 Shutter-TriggerSequenceLength": "1000", "Height": 1200, "555 AO-Block voltage": "false", "640 Shutter-Volts": "0.0000", "PixelType": "GRAY16", "445 Shutter-Block voltage": "false", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "520 Shutter-TriggerSequenceLength": "1000", "COM9-Name": "COM9", "Core-SLM": "", "555 AO-Volts": "4.5000", "555 Shutter-Block voltage": "false", "XYStage-Speed": "1800.0000", "520 AO-MaxVolts": "5.0000", "528 Shutter-SupportsTriggering": "0", "COM1-Description": "Serial port driver (boost:asio)", "PRIME-MetadataEnabled": "No", "520 Shutter-MinVolts": "0.0000", "470 Shutter-Sequenceable": "1", "PRIME-PreampDelay": "0", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-MinVolts": "0.0000", "ChannelIndex": 1, "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Core-AutoShutter": "1", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "Exposure-ms": 160, "PRIME-PP 2 ENABLED": "Yes", "640 Shutter-TriggerSampleRate": "100000", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "470 Shutter-IOChannel": "Dev1/ao10", "405 AO-Demo": "No", "PixelSizeUm": 1.1, "470 AO-Demo": "No", "470 Shutter-MaxVolts": "5.0000", "Vincent-D3-Name": "Vincent-D3", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FileName": "img_000000000_Il-T_005.tif", "LudlController-Reset": "Operate", "PRIME-Exposure": "160.00", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "445-Name": "DA Shutter", "555 Shutter-Name": "AnalogIO", "520 Shutter-TriggerSampleRate": "100000", "445 Shutter-IOChannel": "Dev1/ao9", "PRIME-TransposeCorrection": "0", "XYStage-StepSize": "0.1000", "445-Description": "DA device that is used as a shutter", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 AO-IOChannel": "Dev1/ao7", "520 Shutter-Name": "AnalogIO", "COM3-DataBits": "8", "640 AO-Volts": "0.0000", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "555 Shutter-IOChannel": "Dev1/ao13", "WaitInterval": null, "520 Shutter-Sequenceable": "1", "YPositionUm": -180, "445-State": "0", "445-DA Device": "445 Shutter", "PRIME-CircularBufferAutoSize": "ON", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "555 AO-TriggerSampleRate": "100000", "445 Shutter-Volts": "0.0000", "XLIGHT Hub-Port": "COM3", "Core-AutoFocus": "", "COM4-Handshaking": "Off", "528 Shutter-MinVolts": "0.0000", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "445 Shutter-Name": "AnalogIO", "528 Shutter-Sequenceable": "1", "XYStage-TransposeMirrorX": "0", "XYStage-TransposeMirrorY": "0", "PRIME-ReadoutRate": "100MHz 16bit", "XYStage-Name": "Stage", "640 AO-SupportsTriggering": "0", "640-Name": "DA Shutter", "640 Shutter-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "640 AO-Block voltage": "false", "445 Shutter-Sequenceable": "1", "470 AO-MaxVolts": "5.0000", "COM4-BaudRate": "9600", "470-Description": "DA device that is used as a shutter", "520 Shutter-Description": "NI DAC", "PRIME-CCDTemperature": "-14.8500", "Time": "2019-12-18 23:10:59 -0500", "640 AO-TriggerSequenceLength": "1000", "470 AO-Name": "AnalogIO", "XLIGHT Disk slider-Label": "Disk Pos 40um", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "Binning": "1x1", "TE2000-ModelType": "1", "COM3-AnswerTimeout": "500.0000", "555 Shutter-SupportsTriggering": "0", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "TE2000-Port": "COM4", "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Description": "NI DAC", "COM4-AnswerTimeout": "500.0000", "COM1-DelayBetweenCharsMs": "0.0000", "FilterBlock-Label": "Confocal", "445 AO-Name": "AnalogIO", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-Timing-ReadoutTimeNs": "24672000.0000", "528 AO-Demo": "No", "520 AO-Sequenceable": "1", "555-Description": "DA device that is used as a shutter", "405-DA Device": "405 Shutter", "405 AO-TriggerSequenceLength": "1000", "640 AO-MinVolts": "0.0000", "640 Shutter-Block voltage": "false", "FrameIndex": 0, "PRIME-Port": "CMOS", "555 AO-MinVolts": "0.0000", "COM3-Description": "Serial port driver (boost:asio)", "528 AO-Description": "NI DAC", "555 AO-IOChannel": "Dev1/ao6", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "555 AO-TriggerInputLine": "/Dev1/PFI0", "PRIME-ActualInterval-ms": "390.5200", "LudlController-Port": "COM9", "445 Shutter-Demo": "No", "PRIME-Trigger-Expose Out-Mux": "1", "SliceIndex": 5, "PRIME-CCDTemperatureSetPoint": "-15.0000", "405 Shutter-MinVolts": "0.0000", "PRIME-ExposeOutMode": "First Row", "AxisPositions": null, "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Volts": "5.0000", "528-Name": "DA Shutter", "555-State": "1", "XLIGHT Dichroic-Label": "QUAD", "445 AO-Sequenceable": "1", "405-Name": "DA Shutter", "405 AO-IOChannel": "Dev1/ao0", "Core-Camera": "PRIME", "Focus-Name": "Focus", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "405 Shutter-IOChannel": "Dev1/ao8", "640 AO-Demo": "No", "640 Shutter-SupportsTriggering": "0", "445 AO-Demo": "No", "555-DA Device": "555 Shutter", "470 Shutter-Description": "NI DAC", "528 AO-Volts": "0.0000", "COM1-DataBits": "8", "Vincent-D3-Address": "x", "520 AO-SupportsTriggering": "0", "405-Description": "DA device that is used as a shutter", "640 Shutter-MinVolts": "0.0000", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "405 AO-Name": "AnalogIO", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "640 AO-TriggerInputLine": "/Dev1/PFI0", "405 AO-TriggerInputLine": "/Dev1/PFI0", "640-Description": "DA device that is used as a shutter", "445 Shutter-TriggerSampleRate": "100000", "470 Shutter-TriggerSequenceLength": "1000", "COM1-StopBits": "1", "520 Shutter-SupportsTriggering": "0", "405-State": "0", "Core-Initialize": "1", "XLIGHT Emission Wheel-State": "3", "520 AO-Description": "NI DAC", "528 AO-TriggerInputLine": "/Dev1/PFI0", "445 Shutter-MaxVolts": "5.0000", "COM9-Handshaking": "Off", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "XLIGHT Spin motor-Label": "On (spinning)", "PRIME-PP 5 ENABLED": "No", "UUID": "93908fb7-a5d7-411b-9713-493359f6cab5", "PRIME-TriggerMode": "Internal Trigger", "COM1-Handshaking": "Off", "PRIME-Timing-PostTriggerDelayNs": "49344000.0000", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "Focus-Position": "17741.5000", "528 Shutter-Block voltage": "false", "Channel": "Il-T", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "555 Shutter-Demo": "No", "405 Shutter-Demo": "No", "528 AO-Name": "AnalogIO", "520-State": "0", "Core-Galvo": "", "405 Shutter-TriggerSampleRate": "100000", "Focus-FocusStepSize_(nm)": "100", "PRIME-PixelType": "16bit", "COM4-DataBits": "8", "470 AO-IOChannel": "Dev1/ao2", "520 AO-MinVolts": "0.0000", "COM9-Parity": "None", "PRIME-CircularBufferEnabled": "ON", "PRIME-PP 6 Reset": "No", "640 AO-Description": "NI DAC", "Source": "PRIME", "445 AO-MinVolts": "0.0000", "PIEZO-Name": "Stage", "445 AO-Description": "NI DAC", "PRIME-CircularBufferFrameCount": "46", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "520 AO-Volts": "0.0000", "528 Shutter-TriggerSampleRate": "100000", "XPositionUm": 15712.1, "OpticalPath-State": "4", "PRIME-PP 3 THRESHOLD": "80", "PRIME-PVCAM Version": "3.7.1", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Gain": "1-HDR", "640 Shutter-TriggerSequenceLength": "1000", "528 AO-SupportsTriggering": "0", "445 AO-TriggerInputLine": "/Dev1/PFI0", "OpticalPath-Name": "OpticalPath", "PRIME-PP 4 ENABLED": "Yes", "PRIME-PVCAM Adapter Version": "1.3.29", "COM3-Name": "COM3", "405 AO-MaxVolts": "5.0000", "520 Shutter-Block voltage": "false", "PRIME-Timing-ClearingTimeNs": "49344000.0000", "Core-Focus": "PIEZO", "528 AO-Block voltage": "false", "TE2000-Description": "Nikon TE2000 hub", "528 AO-MinVolts": "0.0000", "COM4-Parity": "None", "Summary": { "Slices": 11, "ChContrastMin": [ 0, 0, 0, 0, 0, 0 ], "PixelAspect": 1, "SlicesFirst": true, "Interval_ms": 0, "Time": "2019-12-18 23:10:50 -0500", "Prefix": "seq_5", "InitialPositionList": [ { "Label": "Pos0", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.21], "Focus": [17741.5], "XYStage": [ 44542.200000000004, 31636.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos1", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.22], "Focus": [17741.4], "XYStage": [ 43801.100000000006, 31637.9 ] }, "GridColumnIndex": 0 }, { "Label": "Pos2", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.5], "XYStage": [ 38561.3, 32040.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos3", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.185], "Focus": [17741.3], "XYStage": [ 37698.4, 32041.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos4", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.16], "Focus": [17741.5], "XYStage": [ 32375.4, 31758.9 ] }, "GridColumnIndex": 0 }, { "Label": "Pos5", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.17000000000002], "Focus": [17741.3], "XYStage": [ 31770.7, 31758.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos6", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.4], "XYStage": [ 26536.800000000003, 31068.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos7", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.205], "Focus": [17741.4], "XYStage": [ 25420.2, 31068.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos8", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.85], "Focus": [17741.3], "XYStage": [ 21059, 31508 ] }, "GridColumnIndex": 0 }, { "Label": "Pos9", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.82], "Focus": [17741.3], "XYStage": [ 19731.9, 31506.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos10", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.79], "Focus": [17741.4], "XYStage": [ 14528.5, 32007.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos11", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.83], "Focus": [17741.2], "XYStage": [ 13830.300000000001, 32009.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos12", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.79], "Focus": [17741.3], "XYStage": [ 8775.300000000001, 32017.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos13", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.815], "Focus": [17741.3], "XYStage": [ 8109.200000000001, 32008.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos14", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.805], "Focus": [17741.3], "XYStage": [ 3483.3, 31908.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos15", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.82], "Focus": [17741.2], "XYStage": [ 2686.8, 31903.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos16", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [211.48000000000002], "Focus": [17741.1], "XYStage": [ -1978.9, 31973.600000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos17", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [211.51500000000001], "Focus": [17741.1], "XYStage": [ -2957.8, 31982.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos18", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.195], "Focus": [17741.5], "XYStage": [ 47747.4, -576.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos19", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.23000000000002], "Focus": [17741.6], "XYStage": [ 47053.9, -576.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos20", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.205], "Focus": [17741.4], "XYStage": [ 41871.200000000004, -479.8 ] }, "GridColumnIndex": 0 }, { "Label": "Pos21", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.4], "XYStage": [ 40882.600000000006, -392 ] }, "GridColumnIndex": 0 }, { "Label": "Pos22", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.24], "Focus": [17741.4], "XYStage": [ 35905.6, -135.3 ] }, "GridColumnIndex": 0 }, { "Label": "Pos23", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.195], "Focus": [17741.5], "XYStage": [ 34990.9, -132.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos24", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.46], "Focus": [17741.4], "XYStage": [ 29678.100000000002, -958.1 ] }, "GridColumnIndex": 0 }, { "Label": "Pos25", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.44], "Focus": [17741.5], "XYStage": [ 28983.600000000002, -939.4000000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos26", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.475], "Focus": [17741.5], "XYStage": [ 23019.7, -122 ] }, "GridColumnIndex": 0 }, { "Label": "Pos27", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.44], "Focus": [17741.7], "XYStage": [ 22428, -116.80000000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos28", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.46], "Focus": [17741.7], "XYStage": [ 16364.900000000001, -181.10000000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos29", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.48000000000002], "Focus": [17741.5], "XYStage": [ 15712.1, -180 ] }, "GridColumnIndex": 0 }, { "Label": "Pos30", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.45000000000002], "Focus": [17741.7], "XYStage": [ 10346.1, 77.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos31", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.49], "Focus": [17741.4], "XYStage": [ 9755.5, 85.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos32", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.475], "Focus": [17741.5], "XYStage": [ 4775.5, 293.90000000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos33", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.445], "Focus": [17741.4], "XYStage": [ 3954.9, 293.90000000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos34", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.455], "Focus": [17741.4], "XYStage": [ -1324.9, 545.6 ] }, "GridColumnIndex": 0 }, { "Label": "Pos35", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.445], "Focus": [17741.3], "XYStage": [ -2097, 576.6 ] }, "GridColumnIndex": 0 } ], "z-step_um": 10, "GridRow": 0, "BitDepth": 16, "Depth": 2, "Channels": 6, "Source": "Micro-Manager", "ChColors": [ -16711681, -256, -65281, -1, -1, -16776961 ], "MetadataVersion": 10, "KeepShutterOpenChannels": true, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536, 65536 ], "CustomIntervals_ms": [], "Height": 1200, "MicroManagerVersion": "1.4.23 20180219", "GridColumn": 0, "UUID": "67598d21-5db5-4842-ab60-f506f7b42c7c", "PixelType": "GRAY16", "Width": 1200, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\Yan\\20191218-YC113-S7S8-miseq", "Frames": 1, "Comment": "", "UserName": "Nikon", "PixelSize_um": 1.1, "ROI": [ 0, 0, 1200, 1200 ], "Date": "2019-12-18", "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC", "P-DAPI-high" ], "KeepShutterOpenSlices": true, "IJType": 1, "ComputerName": "ZadorConfocal2", "Positions": 36, "PositionIndex": 29, "CameraTimeout": 20000, "TimeFirst": false }, "640 AO-Name": "AnalogIO", "Vincent-D3-Command": "Close" }, "FrameKey-0-1-6": { "COM4-StopBits": "1", "528 AO-MaxVolts": "5.0000", "XLIGHT Excitation Wheel-State": "1", "470 Shutter-Name": "AnalogIO", "Core-Shutter": "555", "555 AO-Demo": "No", "XLIGHT Dichroic-State": "2", "XYStage-StepSize-Y": "0.1000", "ZPositionUm": 228.48000000000002, "XYStage-StepSize-X": "0.1000", "PRIME-FirmwareVersion": "20.28", "PRIME-ClearCycles": "2", "520-Name": "DA Shutter", "405 Shutter-Name": "AnalogIO", "PIEZO-StepSize": "0.0050", "PRIME-ClearMode": "Pre-Exposure", "470 AO-MinVolts": "0.0000", "405 AO-SupportsTriggering": "1", "CameraChannelIndex": 0, "XYStage-StartSpeed": "500.0000", "COM9-StopBits": "2", "405 AO-Description": "NI DAC", "COM4-DelayBetweenCharsMs": "0.0000", "445 AO-Block voltage": "false", "520 AO-TriggerInputLine": "/Dev1/PFI0", "555 AO-Description": "NI DAC", "520 AO-TriggerSampleRate": "100000", "COM4-Verbose": "1", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM4-Name": "COM4", "405 Shutter-Volts": "0.0000", "Vincent-D3-Port": "COM1", "PRIME-PP 4 MIN ADU AFFECTED": "200", "470 Shutter-MinVolts": "0.0000", "PRIME-PP 3 ENABLED": "Yes", "470 AO-Description": "NI DAC", "PRIME-Description": "PVCAM API device adapter", "520 Shutter-MaxVolts": "5.0000", "520 Shutter-Volts": "0.0000", "528 AO-Sequenceable": "1", "PRIME-Binning": "1x1", "555 Shutter-MaxVolts": "5.0000", "528 Shutter-TriggerSequenceLength": "1000", "405 Shutter-Block voltage": "false", "XLIGHT Spin motor-State": "1", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "Vincent-D3-Time to open (ms)": "35.0000", "Vincent-D3-Time to close (ms)": "35.0000", "445 AO-TriggerSampleRate": "100000", "640 Shutter-IOChannel": "Dev1/ao14", "555 AO-Sequenceable": "1", "520 AO-Name": "AnalogIO", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "470 AO-Block voltage": "false", "COM1-Verbose": "1", "COM9-AnswerTimeout": "10000.0000", "528 Shutter-Name": "AnalogIO", "PRIME-Trigger Timeout (secs)": "10", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "NextFrame": 0, "470 AO-TriggerSequenceLength": "1000", "PRIME-TransposeXY": "0", "445 AO-TriggerSequenceLength": "1000", "COM1-Name": "COM1", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "COM9-DataBits": "8", "COM1-AnswerTimeout": "500.0000", "Camera": "", "TE2000-Version": "V2.50-V2.50-V2.10", "528 Shutter-Volts": "0.0000", "XLIGHT Hub-Name": "XLIGHT Hub", "PIEZO-Description": "Ludl stage driver adapter", "PositionName": "Pos29", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSampleRate": "100000", "PRIME-FanSpeedSetpoint": "High", "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "405 AO-TriggerSampleRate": "100000", "470 Shutter-Block voltage": "false", "470-State": "0", "COM3-Parity": "None", "405 AO-Volts": "0.0000", "PRIME-TransposeMirrorY": "0", "PRIME-ChipName": "GS144BSI", "470 AO-Volts": "0.0000", "PRIME-TransposeMirrorX": "0", "445 Shutter-SupportsTriggering": "0", "470 Shutter-SupportsTriggering": "0", "XLIGHT Hub-Description": "XLIGHT hub", "PRIME-PreampOffLimit": "10000", "470 AO-Sequenceable": "1", "528 Shutter-MaxVolts": "5.0000", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "Core-XYStage": "XYStage", "470 AO-TriggerSampleRate": "100000", "PRIME-Timing-PreTriggerDelayNs": "24672000.0000", "528-DA Device": "528 Shutter", "Focus-Description": "Nikon TE2000 focus adapter", "FilterBlock-State": "5", "520 AO-Demo": "No", "COM3-DelayBetweenCharsMs": "0.0000", "470 Shutter-Volts": "0.0000", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "PRIME-PP 2 THRESHOLD": "125", "PRIME-PP 4 THRESHOLD": "75", "XYStage-Acceleration": "4.0000", "470 Shutter-Demo": "No", "PRIME-Name": "PMPCIECam00", "640-DA Device": "640 Shutter", "405 AO-Block voltage": "false", "ElapsedTime-ms": 1199905, "PRIME-Offset": "100", "445 AO-IOChannel": "Dev1/ao1", "640 AO-MaxVolts": "5.0000", "PositionIndex": 29, "PRIME-PP 1 ENABLED": "Yes", "COM1-BaudRate": "9600", "640-State": "0", "COM3-Verbose": "1", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "LudlController-TransmissionDelay": "0", "PRIME-FullWellCapacity": "62000", "PRIME-SMARTStreamingEnabled": "No", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "640 Shutter-Description": "NI DAC", "528-Description": "DA device that is used as a shutter", "COM4-Description": "Serial port driver (boost:asio)", "405 Shutter-Description": "NI DAC", "TE2000-Name": "TE2000", "528 Shutter-Description": "NI DAC", "COM9-Description": "Serial port driver (boost:asio)", "LudlController-Name": "LudlController", "445 Shutter-TriggerSequenceLength": "1000", "PRIME-SerialNumber": "A18B203008", "445 AO-Volts": "0.0000", "PRIME-X-dimension": "1200", "PRIME-ShutterCloseDelay": "0", "XLIGHT Disk slider-State": "2", "Position": "Default", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 AO-IOChannel": "Dev1/ao5", "520 AO-IOChannel": "Dev1/ao4", "528 AO-TriggerSequenceLength": "1000", "405 Shutter-MaxVolts": "5.0000", "470-DA Device": "470 Shutter", "PRIME-FTCapable": "No", "Core-ChannelGroup": "Channels", "555 AO-SupportsTriggering": "0", "PRIME-Y-dimension": "1200", "Frame": 0, "555 Shutter-Sequenceable": "1", "PRIME-Timing-ExposureTimeNs": "499998000.0000", "Core-ImageProcessor": "", "528-State": "0", "520 AO-Block voltage": "false", "XLIGHT Excitation Wheel-Label": "QUAD", "640 Shutter-Demo": "No", "COM3-StopBits": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "520 Shutter-IOChannel": "Dev1/ao11", "Width": 1200, "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-SupportsTriggering": "0", "640 AO-TriggerSampleRate": "100000", "520-Description": "DA device that is used as a shutter", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "405 Shutter-Sequenceable": "1", "405 Shutter-SupportsTriggering": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-TriggerSampleRate": "100000", "640 Shutter-MaxVolts": "5.0000", "555 AO-MaxVolts": "5.0000", "520 AO-TriggerSequenceLength": "1000", "COM3-BaudRate": "9600", "COM9-BaudRate": "9600", "Vincent-D3-Channel #": "Ch. #1", "PRIME-PMode": "Normal", "OpticalPath-Label": "Left(100)", "BitDepth": 16, "555 AO-TriggerSequenceLength": "1000", "445 Shutter-Description": "NI DAC", "405 AO-Sequenceable": "1", "PRIME-AcquisitionMethod": "Callbacks", "555 AO-Name": "AnalogIO", "PRIME-ShutterMode": "Pre-Exposure", "COM3-Handshaking": "Off", "445 AO-SupportsTriggering": "0", "ROI": "0-0-1200-1200", "COM9-Verbose": "1", "PRIME-Color": "OFF", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "PRIME-ShutterOpenDelay": "0", "COM1-Parity": "None", "Slice": 6, "SlicePosition": 10, "COM9-DelayBetweenCharsMs": "0.0000", "555-Name": "DA Shutter", "PIEZO-LudlSingleAxisName": "B", "XLIGHT Emission Wheel-Label": "585/11(T)", "640 Shutter-Name": "AnalogIO", "640 AO-Sequenceable": "1", "520 Shutter-Demo": "No", "528 Shutter-Demo": "No", "470-Name": "DA Shutter", "520-DA Device": "520 Shutter", "XYStage-Description": "Ludl XY stage driver adapter", "445 Shutter-MinVolts": "0.0000", "528 AO-TriggerSampleRate": "100000", "405 AO-MinVolts": "0.0000", "405 Shutter-TriggerSequenceLength": "1000", "Height": 1200, "555 AO-Block voltage": "false", "640 Shutter-Volts": "0.0000", "PixelType": "GRAY16", "445 Shutter-Block voltage": "false", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "520 Shutter-TriggerSequenceLength": "1000", "COM9-Name": "COM9", "Core-SLM": "", "555 AO-Volts": "4.5000", "555 Shutter-Block voltage": "false", "XYStage-Speed": "1800.0000", "520 AO-MaxVolts": "5.0000", "528 Shutter-SupportsTriggering": "0", "COM1-Description": "Serial port driver (boost:asio)", "PRIME-MetadataEnabled": "No", "520 Shutter-MinVolts": "0.0000", "470 Shutter-Sequenceable": "1", "PRIME-PreampDelay": "0", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-MinVolts": "0.0000", "ChannelIndex": 1, "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Core-AutoShutter": "1", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "Exposure-ms": 160, "PRIME-PP 2 ENABLED": "Yes", "640 Shutter-TriggerSampleRate": "100000", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "470 Shutter-IOChannel": "Dev1/ao10", "405 AO-Demo": "No", "PixelSizeUm": 1.1, "470 AO-Demo": "No", "470 Shutter-MaxVolts": "5.0000", "Vincent-D3-Name": "Vincent-D3", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FileName": "img_000000000_Il-T_006.tif", "LudlController-Reset": "Operate", "PRIME-Exposure": "160.00", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "445-Name": "DA Shutter", "555 Shutter-Name": "AnalogIO", "520 Shutter-TriggerSampleRate": "100000", "445 Shutter-IOChannel": "Dev1/ao9", "PRIME-TransposeCorrection": "0", "XYStage-StepSize": "0.1000", "445-Description": "DA device that is used as a shutter", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 AO-IOChannel": "Dev1/ao7", "520 Shutter-Name": "AnalogIO", "COM3-DataBits": "8", "640 AO-Volts": "0.0000", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "555 Shutter-IOChannel": "Dev1/ao13", "WaitInterval": null, "520 Shutter-Sequenceable": "1", "YPositionUm": -180, "445-State": "0", "445-DA Device": "445 Shutter", "PRIME-CircularBufferAutoSize": "ON", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "555 AO-TriggerSampleRate": "100000", "445 Shutter-Volts": "0.0000", "XLIGHT Hub-Port": "COM3", "Core-AutoFocus": "", "COM4-Handshaking": "Off", "528 Shutter-MinVolts": "0.0000", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "445 Shutter-Name": "AnalogIO", "528 Shutter-Sequenceable": "1", "XYStage-TransposeMirrorX": "0", "XYStage-TransposeMirrorY": "0", "PRIME-ReadoutRate": "100MHz 16bit", "XYStage-Name": "Stage", "640 AO-SupportsTriggering": "0", "640-Name": "DA Shutter", "640 Shutter-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "640 AO-Block voltage": "false", "445 Shutter-Sequenceable": "1", "470 AO-MaxVolts": "5.0000", "COM4-BaudRate": "9600", "470-Description": "DA device that is used as a shutter", "520 Shutter-Description": "NI DAC", "PRIME-CCDTemperature": "-14.8500", "Time": "2019-12-18 23:11:00 -0500", "640 AO-TriggerSequenceLength": "1000", "470 AO-Name": "AnalogIO", "XLIGHT Disk slider-Label": "Disk Pos 40um", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "Binning": "1x1", "TE2000-ModelType": "1", "COM3-AnswerTimeout": "500.0000", "555 Shutter-SupportsTriggering": "0", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "TE2000-Port": "COM4", "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Description": "NI DAC", "COM4-AnswerTimeout": "500.0000", "COM1-DelayBetweenCharsMs": "0.0000", "FilterBlock-Label": "Confocal", "445 AO-Name": "AnalogIO", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-Timing-ReadoutTimeNs": "24672000.0000", "528 AO-Demo": "No", "520 AO-Sequenceable": "1", "555-Description": "DA device that is used as a shutter", "405-DA Device": "405 Shutter", "405 AO-TriggerSequenceLength": "1000", "640 AO-MinVolts": "0.0000", "640 Shutter-Block voltage": "false", "FrameIndex": 0, "PRIME-Port": "CMOS", "555 AO-MinVolts": "0.0000", "COM3-Description": "Serial port driver (boost:asio)", "528 AO-Description": "NI DAC", "555 AO-IOChannel": "Dev1/ao6", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "555 AO-TriggerInputLine": "/Dev1/PFI0", "PRIME-ActualInterval-ms": "390.5200", "LudlController-Port": "COM9", "445 Shutter-Demo": "No", "PRIME-Trigger-Expose Out-Mux": "1", "SliceIndex": 6, "PRIME-CCDTemperatureSetPoint": "-15.0000", "405 Shutter-MinVolts": "0.0000", "PRIME-ExposeOutMode": "First Row", "AxisPositions": null, "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Volts": "5.0000", "528-Name": "DA Shutter", "555-State": "1", "XLIGHT Dichroic-Label": "QUAD", "445 AO-Sequenceable": "1", "405-Name": "DA Shutter", "405 AO-IOChannel": "Dev1/ao0", "Core-Camera": "PRIME", "Focus-Name": "Focus", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "405 Shutter-IOChannel": "Dev1/ao8", "640 AO-Demo": "No", "640 Shutter-SupportsTriggering": "0", "445 AO-Demo": "No", "555-DA Device": "555 Shutter", "470 Shutter-Description": "NI DAC", "528 AO-Volts": "0.0000", "COM1-DataBits": "8", "Vincent-D3-Address": "x", "520 AO-SupportsTriggering": "0", "405-Description": "DA device that is used as a shutter", "640 Shutter-MinVolts": "0.0000", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "405 AO-Name": "AnalogIO", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "640 AO-TriggerInputLine": "/Dev1/PFI0", "405 AO-TriggerInputLine": "/Dev1/PFI0", "640-Description": "DA device that is used as a shutter", "445 Shutter-TriggerSampleRate": "100000", "470 Shutter-TriggerSequenceLength": "1000", "COM1-StopBits": "1", "520 Shutter-SupportsTriggering": "0", "405-State": "0", "Core-Initialize": "1", "XLIGHT Emission Wheel-State": "3", "520 AO-Description": "NI DAC", "528 AO-TriggerInputLine": "/Dev1/PFI0", "445 Shutter-MaxVolts": "5.0000", "COM9-Handshaking": "Off", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "XLIGHT Spin motor-Label": "On (spinning)", "PRIME-PP 5 ENABLED": "No", "UUID": "15e72455-2069-4548-9b19-fcb5c66d2613", "PRIME-TriggerMode": "Internal Trigger", "COM1-Handshaking": "Off", "PRIME-Timing-PostTriggerDelayNs": "49344000.0000", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "Focus-Position": "17741.5000", "528 Shutter-Block voltage": "false", "Channel": "Il-T", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "555 Shutter-Demo": "No", "405 Shutter-Demo": "No", "528 AO-Name": "AnalogIO", "520-State": "0", "Core-Galvo": "", "405 Shutter-TriggerSampleRate": "100000", "Focus-FocusStepSize_(nm)": "100", "PRIME-PixelType": "16bit", "COM4-DataBits": "8", "470 AO-IOChannel": "Dev1/ao2", "520 AO-MinVolts": "0.0000", "COM9-Parity": "None", "PRIME-CircularBufferEnabled": "ON", "PRIME-PP 6 Reset": "No", "640 AO-Description": "NI DAC", "Source": "PRIME", "445 AO-MinVolts": "0.0000", "PIEZO-Name": "Stage", "445 AO-Description": "NI DAC", "PRIME-CircularBufferFrameCount": "46", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "520 AO-Volts": "0.0000", "528 Shutter-TriggerSampleRate": "100000", "XPositionUm": 15712.1, "OpticalPath-State": "4", "PRIME-PP 3 THRESHOLD": "80", "PRIME-PVCAM Version": "3.7.1", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Gain": "1-HDR", "640 Shutter-TriggerSequenceLength": "1000", "528 AO-SupportsTriggering": "0", "445 AO-TriggerInputLine": "/Dev1/PFI0", "OpticalPath-Name": "OpticalPath", "PRIME-PP 4 ENABLED": "Yes", "PRIME-PVCAM Adapter Version": "1.3.29", "COM3-Name": "COM3", "405 AO-MaxVolts": "5.0000", "520 Shutter-Block voltage": "false", "PRIME-Timing-ClearingTimeNs": "49344000.0000", "Core-Focus": "PIEZO", "528 AO-Block voltage": "false", "TE2000-Description": "Nikon TE2000 hub", "528 AO-MinVolts": "0.0000", "COM4-Parity": "None", "Summary": { "Slices": 11, "ChContrastMin": [ 0, 0, 0, 0, 0, 0 ], "PixelAspect": 1, "SlicesFirst": true, "Interval_ms": 0, "Time": "2019-12-18 23:10:50 -0500", "Prefix": "seq_5", "InitialPositionList": [ { "Label": "Pos0", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.21], "Focus": [17741.5], "XYStage": [ 44542.200000000004, 31636.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos1", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.22], "Focus": [17741.4], "XYStage": [ 43801.100000000006, 31637.9 ] }, "GridColumnIndex": 0 }, { "Label": "Pos2", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.5], "XYStage": [ 38561.3, 32040.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos3", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.185], "Focus": [17741.3], "XYStage": [ 37698.4, 32041.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos4", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.16], "Focus": [17741.5], "XYStage": [ 32375.4, 31758.9 ] }, "GridColumnIndex": 0 }, { "Label": "Pos5", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.17000000000002], "Focus": [17741.3], "XYStage": [ 31770.7, 31758.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos6", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.4], "XYStage": [ 26536.800000000003, 31068.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos7", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.205], "Focus": [17741.4], "XYStage": [ 25420.2, 31068.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos8", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.85], "Focus": [17741.3], "XYStage": [ 21059, 31508 ] }, "GridColumnIndex": 0 }, { "Label": "Pos9", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.82], "Focus": [17741.3], "XYStage": [ 19731.9, 31506.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos10", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.79], "Focus": [17741.4], "XYStage": [ 14528.5, 32007.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos11", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.83], "Focus": [17741.2], "XYStage": [ 13830.300000000001, 32009.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos12", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.79], "Focus": [17741.3], "XYStage": [ 8775.300000000001, 32017.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos13", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.815], "Focus": [17741.3], "XYStage": [ 8109.200000000001, 32008.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos14", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.805], "Focus": [17741.3], "XYStage": [ 3483.3, 31908.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos15", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.82], "Focus": [17741.2], "XYStage": [ 2686.8, 31903.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos16", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [211.48000000000002], "Focus": [17741.1], "XYStage": [ -1978.9, 31973.600000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos17", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [211.51500000000001], "Focus": [17741.1], "XYStage": [ -2957.8, 31982.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos18", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.195], "Focus": [17741.5], "XYStage": [ 47747.4, -576.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos19", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.23000000000002], "Focus": [17741.6], "XYStage": [ 47053.9, -576.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos20", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.205], "Focus": [17741.4], "XYStage": [ 41871.200000000004, -479.8 ] }, "GridColumnIndex": 0 }, { "Label": "Pos21", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.4], "XYStage": [ 40882.600000000006, -392 ] }, "GridColumnIndex": 0 }, { "Label": "Pos22", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.24], "Focus": [17741.4], "XYStage": [ 35905.6, -135.3 ] }, "GridColumnIndex": 0 }, { "Label": "Pos23", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.195], "Focus": [17741.5], "XYStage": [ 34990.9, -132.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos24", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.46], "Focus": [17741.4], "XYStage": [ 29678.100000000002, -958.1 ] }, "GridColumnIndex": 0 }, { "Label": "Pos25", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.44], "Focus": [17741.5], "XYStage": [ 28983.600000000002, -939.4000000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos26", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.475], "Focus": [17741.5], "XYStage": [ 23019.7, -122 ] }, "GridColumnIndex": 0 }, { "Label": "Pos27", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.44], "Focus": [17741.7], "XYStage": [ 22428, -116.80000000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos28", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.46], "Focus": [17741.7], "XYStage": [ 16364.900000000001, -181.10000000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos29", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.48000000000002], "Focus": [17741.5], "XYStage": [ 15712.1, -180 ] }, "GridColumnIndex": 0 }, { "Label": "Pos30", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.45000000000002], "Focus": [17741.7], "XYStage": [ 10346.1, 77.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos31", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.49], "Focus": [17741.4], "XYStage": [ 9755.5, 85.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos32", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.475], "Focus": [17741.5], "XYStage": [ 4775.5, 293.90000000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos33", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.445], "Focus": [17741.4], "XYStage": [ 3954.9, 293.90000000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos34", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.455], "Focus": [17741.4], "XYStage": [ -1324.9, 545.6 ] }, "GridColumnIndex": 0 }, { "Label": "Pos35", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.445], "Focus": [17741.3], "XYStage": [ -2097, 576.6 ] }, "GridColumnIndex": 0 } ], "z-step_um": 10, "GridRow": 0, "BitDepth": 16, "Depth": 2, "Channels": 6, "Source": "Micro-Manager", "ChColors": [ -16711681, -256, -65281, -1, -1, -16776961 ], "MetadataVersion": 10, "KeepShutterOpenChannels": true, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536, 65536 ], "CustomIntervals_ms": [], "Height": 1200, "MicroManagerVersion": "1.4.23 20180219", "GridColumn": 0, "UUID": "67598d21-5db5-4842-ab60-f506f7b42c7c", "PixelType": "GRAY16", "Width": 1200, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\Yan\\20191218-YC113-S7S8-miseq", "Frames": 1, "Comment": "", "UserName": "Nikon", "PixelSize_um": 1.1, "ROI": [ 0, 0, 1200, 1200 ], "Date": "2019-12-18", "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC", "P-DAPI-high" ], "KeepShutterOpenSlices": true, "IJType": 1, "ComputerName": "ZadorConfocal2", "Positions": 36, "PositionIndex": 29, "CameraTimeout": 20000, "TimeFirst": false }, "640 AO-Name": "AnalogIO", "Vincent-D3-Command": "Close" }, "FrameKey-0-1-7": { "COM4-StopBits": "1", "528 AO-MaxVolts": "5.0000", "XLIGHT Excitation Wheel-State": "1", "470 Shutter-Name": "AnalogIO", "Core-Shutter": "555", "555 AO-Demo": "No", "XLIGHT Dichroic-State": "2", "XYStage-StepSize-Y": "0.1000", "ZPositionUm": 238.48000000000002, "XYStage-StepSize-X": "0.1000", "PRIME-FirmwareVersion": "20.28", "PRIME-ClearCycles": "2", "520-Name": "DA Shutter", "405 Shutter-Name": "AnalogIO", "PIEZO-StepSize": "0.0050", "PRIME-ClearMode": "Pre-Exposure", "470 AO-MinVolts": "0.0000", "405 AO-SupportsTriggering": "1", "CameraChannelIndex": 0, "XYStage-StartSpeed": "500.0000", "COM9-StopBits": "2", "405 AO-Description": "NI DAC", "COM4-DelayBetweenCharsMs": "0.0000", "445 AO-Block voltage": "false", "520 AO-TriggerInputLine": "/Dev1/PFI0", "555 AO-Description": "NI DAC", "520 AO-TriggerSampleRate": "100000", "COM4-Verbose": "1", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM4-Name": "COM4", "405 Shutter-Volts": "0.0000", "Vincent-D3-Port": "COM1", "PRIME-PP 4 MIN ADU AFFECTED": "200", "470 Shutter-MinVolts": "0.0000", "PRIME-PP 3 ENABLED": "Yes", "470 AO-Description": "NI DAC", "PRIME-Description": "PVCAM API device adapter", "520 Shutter-MaxVolts": "5.0000", "520 Shutter-Volts": "0.0000", "528 AO-Sequenceable": "1", "PRIME-Binning": "1x1", "555 Shutter-MaxVolts": "5.0000", "528 Shutter-TriggerSequenceLength": "1000", "405 Shutter-Block voltage": "false", "XLIGHT Spin motor-State": "1", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "Vincent-D3-Time to open (ms)": "35.0000", "Vincent-D3-Time to close (ms)": "35.0000", "445 AO-TriggerSampleRate": "100000", "640 Shutter-IOChannel": "Dev1/ao14", "555 AO-Sequenceable": "1", "520 AO-Name": "AnalogIO", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "470 AO-Block voltage": "false", "COM1-Verbose": "1", "COM9-AnswerTimeout": "10000.0000", "528 Shutter-Name": "AnalogIO", "PRIME-Trigger Timeout (secs)": "10", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "NextFrame": 0, "470 AO-TriggerSequenceLength": "1000", "PRIME-TransposeXY": "0", "445 AO-TriggerSequenceLength": "1000", "COM1-Name": "COM1", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "COM9-DataBits": "8", "COM1-AnswerTimeout": "500.0000", "Camera": "", "TE2000-Version": "V2.50-V2.50-V2.10", "528 Shutter-Volts": "0.0000", "XLIGHT Hub-Name": "XLIGHT Hub", "PIEZO-Description": "Ludl stage driver adapter", "PositionName": "Pos29", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSampleRate": "100000", "PRIME-FanSpeedSetpoint": "High", "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "405 AO-TriggerSampleRate": "100000", "470 Shutter-Block voltage": "false", "470-State": "0", "COM3-Parity": "None", "405 AO-Volts": "0.0000", "PRIME-TransposeMirrorY": "0", "PRIME-ChipName": "GS144BSI", "470 AO-Volts": "0.0000", "PRIME-TransposeMirrorX": "0", "445 Shutter-SupportsTriggering": "0", "470 Shutter-SupportsTriggering": "0", "XLIGHT Hub-Description": "XLIGHT hub", "PRIME-PreampOffLimit": "10000", "470 AO-Sequenceable": "1", "528 Shutter-MaxVolts": "5.0000", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "Core-XYStage": "XYStage", "470 AO-TriggerSampleRate": "100000", "PRIME-Timing-PreTriggerDelayNs": "24672000.0000", "528-DA Device": "528 Shutter", "Focus-Description": "Nikon TE2000 focus adapter", "FilterBlock-State": "5", "520 AO-Demo": "No", "COM3-DelayBetweenCharsMs": "0.0000", "470 Shutter-Volts": "0.0000", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "PRIME-PP 2 THRESHOLD": "125", "PRIME-PP 4 THRESHOLD": "75", "XYStage-Acceleration": "4.0000", "470 Shutter-Demo": "No", "PRIME-Name": "PMPCIECam00", "640-DA Device": "640 Shutter", "405 AO-Block voltage": "false", "ElapsedTime-ms": 1200306, "PRIME-Offset": "100", "445 AO-IOChannel": "Dev1/ao1", "640 AO-MaxVolts": "5.0000", "PositionIndex": 29, "PRIME-PP 1 ENABLED": "Yes", "COM1-BaudRate": "9600", "640-State": "0", "COM3-Verbose": "1", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "LudlController-TransmissionDelay": "0", "PRIME-FullWellCapacity": "62000", "PRIME-SMARTStreamingEnabled": "No", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "640 Shutter-Description": "NI DAC", "528-Description": "DA device that is used as a shutter", "COM4-Description": "Serial port driver (boost:asio)", "405 Shutter-Description": "NI DAC", "TE2000-Name": "TE2000", "528 Shutter-Description": "NI DAC", "COM9-Description": "Serial port driver (boost:asio)", "LudlController-Name": "LudlController", "445 Shutter-TriggerSequenceLength": "1000", "PRIME-SerialNumber": "A18B203008", "445 AO-Volts": "0.0000", "PRIME-X-dimension": "1200", "PRIME-ShutterCloseDelay": "0", "XLIGHT Disk slider-State": "2", "Position": "Default", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 AO-IOChannel": "Dev1/ao5", "520 AO-IOChannel": "Dev1/ao4", "528 AO-TriggerSequenceLength": "1000", "405 Shutter-MaxVolts": "5.0000", "470-DA Device": "470 Shutter", "PRIME-FTCapable": "No", "Core-ChannelGroup": "Channels", "555 AO-SupportsTriggering": "0", "PRIME-Y-dimension": "1200", "Frame": 0, "555 Shutter-Sequenceable": "1", "PRIME-Timing-ExposureTimeNs": "499998000.0000", "Core-ImageProcessor": "", "528-State": "0", "520 AO-Block voltage": "false", "XLIGHT Excitation Wheel-Label": "QUAD", "640 Shutter-Demo": "No", "COM3-StopBits": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "520 Shutter-IOChannel": "Dev1/ao11", "Width": 1200, "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-SupportsTriggering": "0", "640 AO-TriggerSampleRate": "100000", "520-Description": "DA device that is used as a shutter", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "405 Shutter-Sequenceable": "1", "405 Shutter-SupportsTriggering": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-TriggerSampleRate": "100000", "640 Shutter-MaxVolts": "5.0000", "555 AO-MaxVolts": "5.0000", "520 AO-TriggerSequenceLength": "1000", "COM3-BaudRate": "9600", "COM9-BaudRate": "9600", "Vincent-D3-Channel #": "Ch. #1", "PRIME-PMode": "Normal", "OpticalPath-Label": "Left(100)", "BitDepth": 16, "555 AO-TriggerSequenceLength": "1000", "445 Shutter-Description": "NI DAC", "405 AO-Sequenceable": "1", "PRIME-AcquisitionMethod": "Callbacks", "555 AO-Name": "AnalogIO", "PRIME-ShutterMode": "Pre-Exposure", "COM3-Handshaking": "Off", "445 AO-SupportsTriggering": "0", "ROI": "0-0-1200-1200", "COM9-Verbose": "1", "PRIME-Color": "OFF", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "PRIME-ShutterOpenDelay": "0", "COM1-Parity": "None", "Slice": 7, "SlicePosition": 20, "COM9-DelayBetweenCharsMs": "0.0000", "555-Name": "DA Shutter", "PIEZO-LudlSingleAxisName": "B", "XLIGHT Emission Wheel-Label": "585/11(T)", "640 Shutter-Name": "AnalogIO", "640 AO-Sequenceable": "1", "520 Shutter-Demo": "No", "528 Shutter-Demo": "No", "470-Name": "DA Shutter", "520-DA Device": "520 Shutter", "XYStage-Description": "Ludl XY stage driver adapter", "445 Shutter-MinVolts": "0.0000", "528 AO-TriggerSampleRate": "100000", "405 AO-MinVolts": "0.0000", "405 Shutter-TriggerSequenceLength": "1000", "Height": 1200, "555 AO-Block voltage": "false", "640 Shutter-Volts": "0.0000", "PixelType": "GRAY16", "445 Shutter-Block voltage": "false", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "520 Shutter-TriggerSequenceLength": "1000", "COM9-Name": "COM9", "Core-SLM": "", "555 AO-Volts": "4.5000", "555 Shutter-Block voltage": "false", "XYStage-Speed": "1800.0000", "520 AO-MaxVolts": "5.0000", "528 Shutter-SupportsTriggering": "0", "COM1-Description": "Serial port driver (boost:asio)", "PRIME-MetadataEnabled": "No", "520 Shutter-MinVolts": "0.0000", "470 Shutter-Sequenceable": "1", "PRIME-PreampDelay": "0", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-MinVolts": "0.0000", "ChannelIndex": 1, "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Core-AutoShutter": "1", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "Exposure-ms": 160, "PRIME-PP 2 ENABLED": "Yes", "640 Shutter-TriggerSampleRate": "100000", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "470 Shutter-IOChannel": "Dev1/ao10", "405 AO-Demo": "No", "PixelSizeUm": 1.1, "470 AO-Demo": "No", "470 Shutter-MaxVolts": "5.0000", "Vincent-D3-Name": "Vincent-D3", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FileName": "img_000000000_Il-T_007.tif", "LudlController-Reset": "Operate", "PRIME-Exposure": "160.00", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "445-Name": "DA Shutter", "555 Shutter-Name": "AnalogIO", "520 Shutter-TriggerSampleRate": "100000", "445 Shutter-IOChannel": "Dev1/ao9", "PRIME-TransposeCorrection": "0", "XYStage-StepSize": "0.1000", "445-Description": "DA device that is used as a shutter", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 AO-IOChannel": "Dev1/ao7", "520 Shutter-Name": "AnalogIO", "COM3-DataBits": "8", "640 AO-Volts": "0.0000", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "555 Shutter-IOChannel": "Dev1/ao13", "WaitInterval": null, "520 Shutter-Sequenceable": "1", "YPositionUm": -180, "445-State": "0", "445-DA Device": "445 Shutter", "PRIME-CircularBufferAutoSize": "ON", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "555 AO-TriggerSampleRate": "100000", "445 Shutter-Volts": "0.0000", "XLIGHT Hub-Port": "COM3", "Core-AutoFocus": "", "COM4-Handshaking": "Off", "528 Shutter-MinVolts": "0.0000", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "445 Shutter-Name": "AnalogIO", "528 Shutter-Sequenceable": "1", "XYStage-TransposeMirrorX": "0", "XYStage-TransposeMirrorY": "0", "PRIME-ReadoutRate": "100MHz 16bit", "XYStage-Name": "Stage", "640 AO-SupportsTriggering": "0", "640-Name": "DA Shutter", "640 Shutter-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "640 AO-Block voltage": "false", "445 Shutter-Sequenceable": "1", "470 AO-MaxVolts": "5.0000", "COM4-BaudRate": "9600", "470-Description": "DA device that is used as a shutter", "520 Shutter-Description": "NI DAC", "PRIME-CCDTemperature": "-14.8500", "Time": "2019-12-18 23:11:00 -0500", "640 AO-TriggerSequenceLength": "1000", "470 AO-Name": "AnalogIO", "XLIGHT Disk slider-Label": "Disk Pos 40um", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "Binning": "1x1", "TE2000-ModelType": "1", "COM3-AnswerTimeout": "500.0000", "555 Shutter-SupportsTriggering": "0", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "TE2000-Port": "COM4", "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Description": "NI DAC", "COM4-AnswerTimeout": "500.0000", "COM1-DelayBetweenCharsMs": "0.0000", "FilterBlock-Label": "Confocal", "445 AO-Name": "AnalogIO", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-Timing-ReadoutTimeNs": "24672000.0000", "528 AO-Demo": "No", "520 AO-Sequenceable": "1", "555-Description": "DA device that is used as a shutter", "405-DA Device": "405 Shutter", "405 AO-TriggerSequenceLength": "1000", "640 AO-MinVolts": "0.0000", "640 Shutter-Block voltage": "false", "FrameIndex": 0, "PRIME-Port": "CMOS", "555 AO-MinVolts": "0.0000", "COM3-Description": "Serial port driver (boost:asio)", "528 AO-Description": "NI DAC", "555 AO-IOChannel": "Dev1/ao6", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "555 AO-TriggerInputLine": "/Dev1/PFI0", "PRIME-ActualInterval-ms": "390.5200", "LudlController-Port": "COM9", "445 Shutter-Demo": "No", "PRIME-Trigger-Expose Out-Mux": "1", "SliceIndex": 7, "PRIME-CCDTemperatureSetPoint": "-15.0000", "405 Shutter-MinVolts": "0.0000", "PRIME-ExposeOutMode": "First Row", "AxisPositions": null, "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Volts": "5.0000", "528-Name": "DA Shutter", "555-State": "1", "XLIGHT Dichroic-Label": "QUAD", "445 AO-Sequenceable": "1", "405-Name": "DA Shutter", "405 AO-IOChannel": "Dev1/ao0", "Core-Camera": "PRIME", "Focus-Name": "Focus", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "405 Shutter-IOChannel": "Dev1/ao8", "640 AO-Demo": "No", "640 Shutter-SupportsTriggering": "0", "445 AO-Demo": "No", "555-DA Device": "555 Shutter", "470 Shutter-Description": "NI DAC", "528 AO-Volts": "0.0000", "COM1-DataBits": "8", "Vincent-D3-Address": "x", "520 AO-SupportsTriggering": "0", "405-Description": "DA device that is used as a shutter", "640 Shutter-MinVolts": "0.0000", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "405 AO-Name": "AnalogIO", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "640 AO-TriggerInputLine": "/Dev1/PFI0", "405 AO-TriggerInputLine": "/Dev1/PFI0", "640-Description": "DA device that is used as a shutter", "445 Shutter-TriggerSampleRate": "100000", "470 Shutter-TriggerSequenceLength": "1000", "COM1-StopBits": "1", "520 Shutter-SupportsTriggering": "0", "405-State": "0", "Core-Initialize": "1", "XLIGHT Emission Wheel-State": "3", "520 AO-Description": "NI DAC", "528 AO-TriggerInputLine": "/Dev1/PFI0", "445 Shutter-MaxVolts": "5.0000", "COM9-Handshaking": "Off", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "XLIGHT Spin motor-Label": "On (spinning)", "PRIME-PP 5 ENABLED": "No", "UUID": "965ae424-7cb9-4a77-a508-74698bb7241b", "PRIME-TriggerMode": "Internal Trigger", "COM1-Handshaking": "Off", "PRIME-Timing-PostTriggerDelayNs": "49344000.0000", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "Focus-Position": "17741.5000", "528 Shutter-Block voltage": "false", "Channel": "Il-T", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "555 Shutter-Demo": "No", "405 Shutter-Demo": "No", "528 AO-Name": "AnalogIO", "520-State": "0", "Core-Galvo": "", "405 Shutter-TriggerSampleRate": "100000", "Focus-FocusStepSize_(nm)": "100", "PRIME-PixelType": "16bit", "COM4-DataBits": "8", "470 AO-IOChannel": "Dev1/ao2", "520 AO-MinVolts": "0.0000", "COM9-Parity": "None", "PRIME-CircularBufferEnabled": "ON", "PRIME-PP 6 Reset": "No", "640 AO-Description": "NI DAC", "Source": "PRIME", "445 AO-MinVolts": "0.0000", "PIEZO-Name": "Stage", "445 AO-Description": "NI DAC", "PRIME-CircularBufferFrameCount": "46", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "520 AO-Volts": "0.0000", "528 Shutter-TriggerSampleRate": "100000", "XPositionUm": 15712.1, "OpticalPath-State": "4", "PRIME-PP 3 THRESHOLD": "80", "PRIME-PVCAM Version": "3.7.1", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Gain": "1-HDR", "640 Shutter-TriggerSequenceLength": "1000", "528 AO-SupportsTriggering": "0", "445 AO-TriggerInputLine": "/Dev1/PFI0", "OpticalPath-Name": "OpticalPath", "PRIME-PP 4 ENABLED": "Yes", "PRIME-PVCAM Adapter Version": "1.3.29", "COM3-Name": "COM3", "405 AO-MaxVolts": "5.0000", "520 Shutter-Block voltage": "false", "PRIME-Timing-ClearingTimeNs": "49344000.0000", "Core-Focus": "PIEZO", "528 AO-Block voltage": "false", "TE2000-Description": "Nikon TE2000 hub", "528 AO-MinVolts": "0.0000", "COM4-Parity": "None", "Summary": { "Slices": 11, "ChContrastMin": [ 0, 0, 0, 0, 0, 0 ], "PixelAspect": 1, "SlicesFirst": true, "Interval_ms": 0, "Time": "2019-12-18 23:10:50 -0500", "Prefix": "seq_5", "InitialPositionList": [ { "Label": "Pos0", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.21], "Focus": [17741.5], "XYStage": [ 44542.200000000004, 31636.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos1", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.22], "Focus": [17741.4], "XYStage": [ 43801.100000000006, 31637.9 ] }, "GridColumnIndex": 0 }, { "Label": "Pos2", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.5], "XYStage": [ 38561.3, 32040.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos3", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.185], "Focus": [17741.3], "XYStage": [ 37698.4, 32041.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos4", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.16], "Focus": [17741.5], "XYStage": [ 32375.4, 31758.9 ] }, "GridColumnIndex": 0 }, { "Label": "Pos5", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.17000000000002], "Focus": [17741.3], "XYStage": [ 31770.7, 31758.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos6", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.4], "XYStage": [ 26536.800000000003, 31068.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos7", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.205], "Focus": [17741.4], "XYStage": [ 25420.2, 31068.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos8", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.85], "Focus": [17741.3], "XYStage": [ 21059, 31508 ] }, "GridColumnIndex": 0 }, { "Label": "Pos9", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.82], "Focus": [17741.3], "XYStage": [ 19731.9, 31506.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos10", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.79], "Focus": [17741.4], "XYStage": [ 14528.5, 32007.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos11", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.83], "Focus": [17741.2], "XYStage": [ 13830.300000000001, 32009.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos12", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.79], "Focus": [17741.3], "XYStage": [ 8775.300000000001, 32017.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos13", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.815], "Focus": [17741.3], "XYStage": [ 8109.200000000001, 32008.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos14", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.805], "Focus": [17741.3], "XYStage": [ 3483.3, 31908.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos15", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.82], "Focus": [17741.2], "XYStage": [ 2686.8, 31903.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos16", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [211.48000000000002], "Focus": [17741.1], "XYStage": [ -1978.9, 31973.600000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos17", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [211.51500000000001], "Focus": [17741.1], "XYStage": [ -2957.8, 31982.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos18", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.195], "Focus": [17741.5], "XYStage": [ 47747.4, -576.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos19", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.23000000000002], "Focus": [17741.6], "XYStage": [ 47053.9, -576.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos20", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.205], "Focus": [17741.4], "XYStage": [ 41871.200000000004, -479.8 ] }, "GridColumnIndex": 0 }, { "Label": "Pos21", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.4], "XYStage": [ 40882.600000000006, -392 ] }, "GridColumnIndex": 0 }, { "Label": "Pos22", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.24], "Focus": [17741.4], "XYStage": [ 35905.6, -135.3 ] }, "GridColumnIndex": 0 }, { "Label": "Pos23", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.195], "Focus": [17741.5], "XYStage": [ 34990.9, -132.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos24", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.46], "Focus": [17741.4], "XYStage": [ 29678.100000000002, -958.1 ] }, "GridColumnIndex": 0 }, { "Label": "Pos25", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.44], "Focus": [17741.5], "XYStage": [ 28983.600000000002, -939.4000000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos26", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.475], "Focus": [17741.5], "XYStage": [ 23019.7, -122 ] }, "GridColumnIndex": 0 }, { "Label": "Pos27", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.44], "Focus": [17741.7], "XYStage": [ 22428, -116.80000000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos28", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.46], "Focus": [17741.7], "XYStage": [ 16364.900000000001, -181.10000000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos29", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.48000000000002], "Focus": [17741.5], "XYStage": [ 15712.1, -180 ] }, "GridColumnIndex": 0 }, { "Label": "Pos30", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.45000000000002], "Focus": [17741.7], "XYStage": [ 10346.1, 77.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos31", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.49], "Focus": [17741.4], "XYStage": [ 9755.5, 85.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos32", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.475], "Focus": [17741.5], "XYStage": [ 4775.5, 293.90000000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos33", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.445], "Focus": [17741.4], "XYStage": [ 3954.9, 293.90000000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos34", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.455], "Focus": [17741.4], "XYStage": [ -1324.9, 545.6 ] }, "GridColumnIndex": 0 }, { "Label": "Pos35", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.445], "Focus": [17741.3], "XYStage": [ -2097, 576.6 ] }, "GridColumnIndex": 0 } ], "z-step_um": 10, "GridRow": 0, "BitDepth": 16, "Depth": 2, "Channels": 6, "Source": "Micro-Manager", "ChColors": [ -16711681, -256, -65281, -1, -1, -16776961 ], "MetadataVersion": 10, "KeepShutterOpenChannels": true, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536, 65536 ], "CustomIntervals_ms": [], "Height": 1200, "MicroManagerVersion": "1.4.23 20180219", "GridColumn": 0, "UUID": "67598d21-5db5-4842-ab60-f506f7b42c7c", "PixelType": "GRAY16", "Width": 1200, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\Yan\\20191218-YC113-S7S8-miseq", "Frames": 1, "Comment": "", "UserName": "Nikon", "PixelSize_um": 1.1, "ROI": [ 0, 0, 1200, 1200 ], "Date": "2019-12-18", "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC", "P-DAPI-high" ], "KeepShutterOpenSlices": true, "IJType": 1, "ComputerName": "ZadorConfocal2", "Positions": 36, "PositionIndex": 29, "CameraTimeout": 20000, "TimeFirst": false }, "640 AO-Name": "AnalogIO", "Vincent-D3-Command": "Close" }, "FrameKey-0-1-8": { "COM4-StopBits": "1", "528 AO-MaxVolts": "5.0000", "XLIGHT Excitation Wheel-State": "1", "470 Shutter-Name": "AnalogIO", "Core-Shutter": "555", "555 AO-Demo": "No", "XLIGHT Dichroic-State": "2", "XYStage-StepSize-Y": "0.1000", "ZPositionUm": 248.48000000000002, "XYStage-StepSize-X": "0.1000", "PRIME-FirmwareVersion": "20.28", "PRIME-ClearCycles": "2", "520-Name": "DA Shutter", "405 Shutter-Name": "AnalogIO", "PIEZO-StepSize": "0.0050", "PRIME-ClearMode": "Pre-Exposure", "470 AO-MinVolts": "0.0000", "405 AO-SupportsTriggering": "1", "CameraChannelIndex": 0, "XYStage-StartSpeed": "500.0000", "COM9-StopBits": "2", "405 AO-Description": "NI DAC", "COM4-DelayBetweenCharsMs": "0.0000", "445 AO-Block voltage": "false", "520 AO-TriggerInputLine": "/Dev1/PFI0", "555 AO-Description": "NI DAC", "520 AO-TriggerSampleRate": "100000", "COM4-Verbose": "1", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM4-Name": "COM4", "405 Shutter-Volts": "0.0000", "Vincent-D3-Port": "COM1", "PRIME-PP 4 MIN ADU AFFECTED": "200", "470 Shutter-MinVolts": "0.0000", "PRIME-PP 3 ENABLED": "Yes", "470 AO-Description": "NI DAC", "PRIME-Description": "PVCAM API device adapter", "520 Shutter-MaxVolts": "5.0000", "520 Shutter-Volts": "0.0000", "528 AO-Sequenceable": "1", "PRIME-Binning": "1x1", "555 Shutter-MaxVolts": "5.0000", "528 Shutter-TriggerSequenceLength": "1000", "405 Shutter-Block voltage": "false", "XLIGHT Spin motor-State": "1", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "Vincent-D3-Time to open (ms)": "35.0000", "Vincent-D3-Time to close (ms)": "35.0000", "445 AO-TriggerSampleRate": "100000", "640 Shutter-IOChannel": "Dev1/ao14", "555 AO-Sequenceable": "1", "520 AO-Name": "AnalogIO", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "470 AO-Block voltage": "false", "COM1-Verbose": "1", "COM9-AnswerTimeout": "10000.0000", "528 Shutter-Name": "AnalogIO", "PRIME-Trigger Timeout (secs)": "10", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "NextFrame": 0, "470 AO-TriggerSequenceLength": "1000", "PRIME-TransposeXY": "0", "445 AO-TriggerSequenceLength": "1000", "COM1-Name": "COM1", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "COM9-DataBits": "8", "COM1-AnswerTimeout": "500.0000", "Camera": "", "TE2000-Version": "V2.50-V2.50-V2.10", "528 Shutter-Volts": "0.0000", "XLIGHT Hub-Name": "XLIGHT Hub", "PIEZO-Description": "Ludl stage driver adapter", "PositionName": "Pos29", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSampleRate": "100000", "PRIME-FanSpeedSetpoint": "High", "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "405 AO-TriggerSampleRate": "100000", "470 Shutter-Block voltage": "false", "470-State": "0", "COM3-Parity": "None", "405 AO-Volts": "0.0000", "PRIME-TransposeMirrorY": "0", "PRIME-ChipName": "GS144BSI", "470 AO-Volts": "0.0000", "PRIME-TransposeMirrorX": "0", "445 Shutter-SupportsTriggering": "0", "470 Shutter-SupportsTriggering": "0", "XLIGHT Hub-Description": "XLIGHT hub", "PRIME-PreampOffLimit": "10000", "470 AO-Sequenceable": "1", "528 Shutter-MaxVolts": "5.0000", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "Core-XYStage": "XYStage", "470 AO-TriggerSampleRate": "100000", "PRIME-Timing-PreTriggerDelayNs": "24672000.0000", "528-DA Device": "528 Shutter", "Focus-Description": "Nikon TE2000 focus adapter", "FilterBlock-State": "5", "520 AO-Demo": "No", "COM3-DelayBetweenCharsMs": "0.0000", "470 Shutter-Volts": "0.0000", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "PRIME-PP 2 THRESHOLD": "125", "PRIME-PP 4 THRESHOLD": "75", "XYStage-Acceleration": "4.0000", "470 Shutter-Demo": "No", "PRIME-Name": "PMPCIECam00", "640-DA Device": "640 Shutter", "405 AO-Block voltage": "false", "ElapsedTime-ms": 1200759, "PRIME-Offset": "100", "445 AO-IOChannel": "Dev1/ao1", "640 AO-MaxVolts": "5.0000", "PositionIndex": 29, "PRIME-PP 1 ENABLED": "Yes", "COM1-BaudRate": "9600", "640-State": "0", "COM3-Verbose": "1", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "LudlController-TransmissionDelay": "0", "PRIME-FullWellCapacity": "62000", "PRIME-SMARTStreamingEnabled": "No", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "640 Shutter-Description": "NI DAC", "528-Description": "DA device that is used as a shutter", "COM4-Description": "Serial port driver (boost:asio)", "405 Shutter-Description": "NI DAC", "TE2000-Name": "TE2000", "528 Shutter-Description": "NI DAC", "COM9-Description": "Serial port driver (boost:asio)", "LudlController-Name": "LudlController", "445 Shutter-TriggerSequenceLength": "1000", "PRIME-SerialNumber": "A18B203008", "445 AO-Volts": "0.0000", "PRIME-X-dimension": "1200", "PRIME-ShutterCloseDelay": "0", "XLIGHT Disk slider-State": "2", "Position": "Default", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 AO-IOChannel": "Dev1/ao5", "520 AO-IOChannel": "Dev1/ao4", "528 AO-TriggerSequenceLength": "1000", "405 Shutter-MaxVolts": "5.0000", "470-DA Device": "470 Shutter", "PRIME-FTCapable": "No", "Core-ChannelGroup": "Channels", "555 AO-SupportsTriggering": "0", "PRIME-Y-dimension": "1200", "Frame": 0, "555 Shutter-Sequenceable": "1", "PRIME-Timing-ExposureTimeNs": "499998000.0000", "Core-ImageProcessor": "", "528-State": "0", "520 AO-Block voltage": "false", "XLIGHT Excitation Wheel-Label": "QUAD", "640 Shutter-Demo": "No", "COM3-StopBits": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "520 Shutter-IOChannel": "Dev1/ao11", "Width": 1200, "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-SupportsTriggering": "0", "640 AO-TriggerSampleRate": "100000", "520-Description": "DA device that is used as a shutter", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "405 Shutter-Sequenceable": "1", "405 Shutter-SupportsTriggering": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-TriggerSampleRate": "100000", "640 Shutter-MaxVolts": "5.0000", "555 AO-MaxVolts": "5.0000", "520 AO-TriggerSequenceLength": "1000", "COM3-BaudRate": "9600", "COM9-BaudRate": "9600", "Vincent-D3-Channel #": "Ch. #1", "PRIME-PMode": "Normal", "OpticalPath-Label": "Left(100)", "BitDepth": 16, "555 AO-TriggerSequenceLength": "1000", "445 Shutter-Description": "NI DAC", "405 AO-Sequenceable": "1", "PRIME-AcquisitionMethod": "Callbacks", "555 AO-Name": "AnalogIO", "PRIME-ShutterMode": "Pre-Exposure", "COM3-Handshaking": "Off", "445 AO-SupportsTriggering": "0", "ROI": "0-0-1200-1200", "COM9-Verbose": "1", "PRIME-Color": "OFF", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "PRIME-ShutterOpenDelay": "0", "COM1-Parity": "None", "Slice": 8, "SlicePosition": 30, "COM9-DelayBetweenCharsMs": "0.0000", "555-Name": "DA Shutter", "PIEZO-LudlSingleAxisName": "B", "XLIGHT Emission Wheel-Label": "585/11(T)", "640 Shutter-Name": "AnalogIO", "640 AO-Sequenceable": "1", "520 Shutter-Demo": "No", "528 Shutter-Demo": "No", "470-Name": "DA Shutter", "520-DA Device": "520 Shutter", "XYStage-Description": "Ludl XY stage driver adapter", "445 Shutter-MinVolts": "0.0000", "528 AO-TriggerSampleRate": "100000", "405 AO-MinVolts": "0.0000", "405 Shutter-TriggerSequenceLength": "1000", "Height": 1200, "555 AO-Block voltage": "false", "640 Shutter-Volts": "0.0000", "PixelType": "GRAY16", "445 Shutter-Block voltage": "false", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "520 Shutter-TriggerSequenceLength": "1000", "COM9-Name": "COM9", "Core-SLM": "", "555 AO-Volts": "4.5000", "555 Shutter-Block voltage": "false", "XYStage-Speed": "1800.0000", "520 AO-MaxVolts": "5.0000", "528 Shutter-SupportsTriggering": "0", "COM1-Description": "Serial port driver (boost:asio)", "PRIME-MetadataEnabled": "No", "520 Shutter-MinVolts": "0.0000", "470 Shutter-Sequenceable": "1", "PRIME-PreampDelay": "0", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-MinVolts": "0.0000", "ChannelIndex": 1, "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Core-AutoShutter": "1", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "Exposure-ms": 160, "PRIME-PP 2 ENABLED": "Yes", "640 Shutter-TriggerSampleRate": "100000", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "470 Shutter-IOChannel": "Dev1/ao10", "405 AO-Demo": "No", "PixelSizeUm": 1.1, "470 AO-Demo": "No", "470 Shutter-MaxVolts": "5.0000", "Vincent-D3-Name": "Vincent-D3", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FileName": "img_000000000_Il-T_008.tif", "LudlController-Reset": "Operate", "PRIME-Exposure": "160.00", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "445-Name": "DA Shutter", "555 Shutter-Name": "AnalogIO", "520 Shutter-TriggerSampleRate": "100000", "445 Shutter-IOChannel": "Dev1/ao9", "PRIME-TransposeCorrection": "0", "XYStage-StepSize": "0.1000", "445-Description": "DA device that is used as a shutter", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 AO-IOChannel": "Dev1/ao7", "520 Shutter-Name": "AnalogIO", "COM3-DataBits": "8", "640 AO-Volts": "0.0000", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "555 Shutter-IOChannel": "Dev1/ao13", "WaitInterval": null, "520 Shutter-Sequenceable": "1", "YPositionUm": -180, "445-State": "0", "445-DA Device": "445 Shutter", "PRIME-CircularBufferAutoSize": "ON", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "555 AO-TriggerSampleRate": "100000", "445 Shutter-Volts": "0.0000", "XLIGHT Hub-Port": "COM3", "Core-AutoFocus": "", "COM4-Handshaking": "Off", "528 Shutter-MinVolts": "0.0000", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "445 Shutter-Name": "AnalogIO", "528 Shutter-Sequenceable": "1", "XYStage-TransposeMirrorX": "0", "XYStage-TransposeMirrorY": "0", "PRIME-ReadoutRate": "100MHz 16bit", "XYStage-Name": "Stage", "640 AO-SupportsTriggering": "0", "640-Name": "DA Shutter", "640 Shutter-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "640 AO-Block voltage": "false", "445 Shutter-Sequenceable": "1", "470 AO-MaxVolts": "5.0000", "COM4-BaudRate": "9600", "470-Description": "DA device that is used as a shutter", "520 Shutter-Description": "NI DAC", "PRIME-CCDTemperature": "-14.8500", "Time": "2019-12-18 23:11:00 -0500", "640 AO-TriggerSequenceLength": "1000", "470 AO-Name": "AnalogIO", "XLIGHT Disk slider-Label": "Disk Pos 40um", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "Binning": "1x1", "TE2000-ModelType": "1", "COM3-AnswerTimeout": "500.0000", "555 Shutter-SupportsTriggering": "0", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "TE2000-Port": "COM4", "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Description": "NI DAC", "COM4-AnswerTimeout": "500.0000", "COM1-DelayBetweenCharsMs": "0.0000", "FilterBlock-Label": "Confocal", "445 AO-Name": "AnalogIO", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-Timing-ReadoutTimeNs": "24672000.0000", "528 AO-Demo": "No", "520 AO-Sequenceable": "1", "555-Description": "DA device that is used as a shutter", "405-DA Device": "405 Shutter", "405 AO-TriggerSequenceLength": "1000", "640 AO-MinVolts": "0.0000", "640 Shutter-Block voltage": "false", "FrameIndex": 0, "PRIME-Port": "CMOS", "555 AO-MinVolts": "0.0000", "COM3-Description": "Serial port driver (boost:asio)", "528 AO-Description": "NI DAC", "555 AO-IOChannel": "Dev1/ao6", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "555 AO-TriggerInputLine": "/Dev1/PFI0", "PRIME-ActualInterval-ms": "390.5200", "LudlController-Port": "COM9", "445 Shutter-Demo": "No", "PRIME-Trigger-Expose Out-Mux": "1", "SliceIndex": 8, "PRIME-CCDTemperatureSetPoint": "-15.0000", "405 Shutter-MinVolts": "0.0000", "PRIME-ExposeOutMode": "First Row", "AxisPositions": null, "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Volts": "5.0000", "528-Name": "DA Shutter", "555-State": "1", "XLIGHT Dichroic-Label": "QUAD", "445 AO-Sequenceable": "1", "405-Name": "DA Shutter", "405 AO-IOChannel": "Dev1/ao0", "Core-Camera": "PRIME", "Focus-Name": "Focus", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "405 Shutter-IOChannel": "Dev1/ao8", "640 AO-Demo": "No", "640 Shutter-SupportsTriggering": "0", "445 AO-Demo": "No", "555-DA Device": "555 Shutter", "470 Shutter-Description": "NI DAC", "528 AO-Volts": "0.0000", "COM1-DataBits": "8", "Vincent-D3-Address": "x", "520 AO-SupportsTriggering": "0", "405-Description": "DA device that is used as a shutter", "640 Shutter-MinVolts": "0.0000", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "405 AO-Name": "AnalogIO", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "640 AO-TriggerInputLine": "/Dev1/PFI0", "405 AO-TriggerInputLine": "/Dev1/PFI0", "640-Description": "DA device that is used as a shutter", "445 Shutter-TriggerSampleRate": "100000", "470 Shutter-TriggerSequenceLength": "1000", "COM1-StopBits": "1", "520 Shutter-SupportsTriggering": "0", "405-State": "0", "Core-Initialize": "1", "XLIGHT Emission Wheel-State": "3", "520 AO-Description": "NI DAC", "528 AO-TriggerInputLine": "/Dev1/PFI0", "445 Shutter-MaxVolts": "5.0000", "COM9-Handshaking": "Off", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "XLIGHT Spin motor-Label": "On (spinning)", "PRIME-PP 5 ENABLED": "No", "UUID": "e6d8c686-76c7-48e1-bd6d-faaeaaa8ca80", "PRIME-TriggerMode": "Internal Trigger", "COM1-Handshaking": "Off", "PRIME-Timing-PostTriggerDelayNs": "49344000.0000", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "Focus-Position": "17741.5000", "528 Shutter-Block voltage": "false", "Channel": "Il-T", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "555 Shutter-Demo": "No", "405 Shutter-Demo": "No", "528 AO-Name": "AnalogIO", "520-State": "0", "Core-Galvo": "", "405 Shutter-TriggerSampleRate": "100000", "Focus-FocusStepSize_(nm)": "100", "PRIME-PixelType": "16bit", "COM4-DataBits": "8", "470 AO-IOChannel": "Dev1/ao2", "520 AO-MinVolts": "0.0000", "COM9-Parity": "None", "PRIME-CircularBufferEnabled": "ON", "PRIME-PP 6 Reset": "No", "640 AO-Description": "NI DAC", "Source": "PRIME", "445 AO-MinVolts": "0.0000", "PIEZO-Name": "Stage", "445 AO-Description": "NI DAC", "PRIME-CircularBufferFrameCount": "46", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "520 AO-Volts": "0.0000", "528 Shutter-TriggerSampleRate": "100000", "XPositionUm": 15712.1, "OpticalPath-State": "4", "PRIME-PP 3 THRESHOLD": "80", "PRIME-PVCAM Version": "3.7.1", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Gain": "1-HDR", "640 Shutter-TriggerSequenceLength": "1000", "528 AO-SupportsTriggering": "0", "445 AO-TriggerInputLine": "/Dev1/PFI0", "OpticalPath-Name": "OpticalPath", "PRIME-PP 4 ENABLED": "Yes", "PRIME-PVCAM Adapter Version": "1.3.29", "COM3-Name": "COM3", "405 AO-MaxVolts": "5.0000", "520 Shutter-Block voltage": "false", "PRIME-Timing-ClearingTimeNs": "49344000.0000", "Core-Focus": "PIEZO", "528 AO-Block voltage": "false", "TE2000-Description": "Nikon TE2000 hub", "528 AO-MinVolts": "0.0000", "COM4-Parity": "None", "Summary": { "Slices": 11, "ChContrastMin": [ 0, 0, 0, 0, 0, 0 ], "PixelAspect": 1, "SlicesFirst": true, "Interval_ms": 0, "Time": "2019-12-18 23:10:50 -0500", "Prefix": "seq_5", "InitialPositionList": [ { "Label": "Pos0", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.21], "Focus": [17741.5], "XYStage": [ 44542.200000000004, 31636.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos1", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.22], "Focus": [17741.4], "XYStage": [ 43801.100000000006, 31637.9 ] }, "GridColumnIndex": 0 }, { "Label": "Pos2", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.5], "XYStage": [ 38561.3, 32040.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos3", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.185], "Focus": [17741.3], "XYStage": [ 37698.4, 32041.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos4", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.16], "Focus": [17741.5], "XYStage": [ 32375.4, 31758.9 ] }, "GridColumnIndex": 0 }, { "Label": "Pos5", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.17000000000002], "Focus": [17741.3], "XYStage": [ 31770.7, 31758.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos6", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.4], "XYStage": [ 26536.800000000003, 31068.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos7", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.205], "Focus": [17741.4], "XYStage": [ 25420.2, 31068.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos8", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.85], "Focus": [17741.3], "XYStage": [ 21059, 31508 ] }, "GridColumnIndex": 0 }, { "Label": "Pos9", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.82], "Focus": [17741.3], "XYStage": [ 19731.9, 31506.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos10", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.79], "Focus": [17741.4], "XYStage": [ 14528.5, 32007.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos11", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.83], "Focus": [17741.2], "XYStage": [ 13830.300000000001, 32009.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos12", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.79], "Focus": [17741.3], "XYStage": [ 8775.300000000001, 32017.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos13", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.815], "Focus": [17741.3], "XYStage": [ 8109.200000000001, 32008.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos14", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.805], "Focus": [17741.3], "XYStage": [ 3483.3, 31908.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos15", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.82], "Focus": [17741.2], "XYStage": [ 2686.8, 31903.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos16", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [211.48000000000002], "Focus": [17741.1], "XYStage": [ -1978.9, 31973.600000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos17", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [211.51500000000001], "Focus": [17741.1], "XYStage": [ -2957.8, 31982.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos18", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.195], "Focus": [17741.5], "XYStage": [ 47747.4, -576.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos19", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.23000000000002], "Focus": [17741.6], "XYStage": [ 47053.9, -576.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos20", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.205], "Focus": [17741.4], "XYStage": [ 41871.200000000004, -479.8 ] }, "GridColumnIndex": 0 }, { "Label": "Pos21", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.4], "XYStage": [ 40882.600000000006, -392 ] }, "GridColumnIndex": 0 }, { "Label": "Pos22", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.24], "Focus": [17741.4], "XYStage": [ 35905.6, -135.3 ] }, "GridColumnIndex": 0 }, { "Label": "Pos23", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.195], "Focus": [17741.5], "XYStage": [ 34990.9, -132.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos24", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.46], "Focus": [17741.4], "XYStage": [ 29678.100000000002, -958.1 ] }, "GridColumnIndex": 0 }, { "Label": "Pos25", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.44], "Focus": [17741.5], "XYStage": [ 28983.600000000002, -939.4000000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos26", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.475], "Focus": [17741.5], "XYStage": [ 23019.7, -122 ] }, "GridColumnIndex": 0 }, { "Label": "Pos27", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.44], "Focus": [17741.7], "XYStage": [ 22428, -116.80000000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos28", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.46], "Focus": [17741.7], "XYStage": [ 16364.900000000001, -181.10000000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos29", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.48000000000002], "Focus": [17741.5], "XYStage": [ 15712.1, -180 ] }, "GridColumnIndex": 0 }, { "Label": "Pos30", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.45000000000002], "Focus": [17741.7], "XYStage": [ 10346.1, 77.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos31", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.49], "Focus": [17741.4], "XYStage": [ 9755.5, 85.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos32", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.475], "Focus": [17741.5], "XYStage": [ 4775.5, 293.90000000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos33", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.445], "Focus": [17741.4], "XYStage": [ 3954.9, 293.90000000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos34", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.455], "Focus": [17741.4], "XYStage": [ -1324.9, 545.6 ] }, "GridColumnIndex": 0 }, { "Label": "Pos35", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.445], "Focus": [17741.3], "XYStage": [ -2097, 576.6 ] }, "GridColumnIndex": 0 } ], "z-step_um": 10, "GridRow": 0, "BitDepth": 16, "Depth": 2, "Channels": 6, "Source": "Micro-Manager", "ChColors": [ -16711681, -256, -65281, -1, -1, -16776961 ], "MetadataVersion": 10, "KeepShutterOpenChannels": true, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536, 65536 ], "CustomIntervals_ms": [], "Height": 1200, "MicroManagerVersion": "1.4.23 20180219", "GridColumn": 0, "UUID": "67598d21-5db5-4842-ab60-f506f7b42c7c", "PixelType": "GRAY16", "Width": 1200, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\Yan\\20191218-YC113-S7S8-miseq", "Frames": 1, "Comment": "", "UserName": "Nikon", "PixelSize_um": 1.1, "ROI": [ 0, 0, 1200, 1200 ], "Date": "2019-12-18", "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC", "P-DAPI-high" ], "KeepShutterOpenSlices": true, "IJType": 1, "ComputerName": "ZadorConfocal2", "Positions": 36, "PositionIndex": 29, "CameraTimeout": 20000, "TimeFirst": false }, "640 AO-Name": "AnalogIO", "Vincent-D3-Command": "Close" }, "FrameKey-0-1-9": { "COM4-StopBits": "1", "528 AO-MaxVolts": "5.0000", "XLIGHT Excitation Wheel-State": "1", "470 Shutter-Name": "AnalogIO", "Core-Shutter": "555", "555 AO-Demo": "No", "XLIGHT Dichroic-State": "2", "XYStage-StepSize-Y": "0.1000", "ZPositionUm": 258.48, "XYStage-StepSize-X": "0.1000", "PRIME-FirmwareVersion": "20.28", "PRIME-ClearCycles": "2", "520-Name": "DA Shutter", "405 Shutter-Name": "AnalogIO", "PIEZO-StepSize": "0.0050", "PRIME-ClearMode": "Pre-Exposure", "470 AO-MinVolts": "0.0000", "405 AO-SupportsTriggering": "1", "CameraChannelIndex": 0, "XYStage-StartSpeed": "500.0000", "COM9-StopBits": "2", "405 AO-Description": "NI DAC", "COM4-DelayBetweenCharsMs": "0.0000", "445 AO-Block voltage": "false", "520 AO-TriggerInputLine": "/Dev1/PFI0", "555 AO-Description": "NI DAC", "520 AO-TriggerSampleRate": "100000", "COM4-Verbose": "1", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM4-Name": "COM4", "405 Shutter-Volts": "0.0000", "Vincent-D3-Port": "COM1", "PRIME-PP 4 MIN ADU AFFECTED": "200", "470 Shutter-MinVolts": "0.0000", "PRIME-PP 3 ENABLED": "Yes", "470 AO-Description": "NI DAC", "PRIME-Description": "PVCAM API device adapter", "520 Shutter-MaxVolts": "5.0000", "520 Shutter-Volts": "0.0000", "528 AO-Sequenceable": "1", "PRIME-Binning": "1x1", "555 Shutter-MaxVolts": "5.0000", "528 Shutter-TriggerSequenceLength": "1000", "405 Shutter-Block voltage": "false", "XLIGHT Spin motor-State": "1", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "Vincent-D3-Time to open (ms)": "35.0000", "Vincent-D3-Time to close (ms)": "35.0000", "445 AO-TriggerSampleRate": "100000", "640 Shutter-IOChannel": "Dev1/ao14", "555 AO-Sequenceable": "1", "520 AO-Name": "AnalogIO", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "470 AO-Block voltage": "false", "COM1-Verbose": "1", "COM9-AnswerTimeout": "10000.0000", "528 Shutter-Name": "AnalogIO", "PRIME-Trigger Timeout (secs)": "10", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "NextFrame": 0, "470 AO-TriggerSequenceLength": "1000", "PRIME-TransposeXY": "0", "445 AO-TriggerSequenceLength": "1000", "COM1-Name": "COM1", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "COM9-DataBits": "8", "COM1-AnswerTimeout": "500.0000", "Camera": "", "TE2000-Version": "V2.50-V2.50-V2.10", "528 Shutter-Volts": "0.0000", "XLIGHT Hub-Name": "XLIGHT Hub", "PIEZO-Description": "Ludl stage driver adapter", "PositionName": "Pos29", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSampleRate": "100000", "PRIME-FanSpeedSetpoint": "High", "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "405 AO-TriggerSampleRate": "100000", "470 Shutter-Block voltage": "false", "470-State": "0", "COM3-Parity": "None", "405 AO-Volts": "0.0000", "PRIME-TransposeMirrorY": "0", "PRIME-ChipName": "GS144BSI", "470 AO-Volts": "0.0000", "PRIME-TransposeMirrorX": "0", "445 Shutter-SupportsTriggering": "0", "470 Shutter-SupportsTriggering": "0", "XLIGHT Hub-Description": "XLIGHT hub", "PRIME-PreampOffLimit": "10000", "470 AO-Sequenceable": "1", "528 Shutter-MaxVolts": "5.0000", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "Core-XYStage": "XYStage", "470 AO-TriggerSampleRate": "100000", "PRIME-Timing-PreTriggerDelayNs": "24672000.0000", "528-DA Device": "528 Shutter", "Focus-Description": "Nikon TE2000 focus adapter", "FilterBlock-State": "5", "520 AO-Demo": "No", "COM3-DelayBetweenCharsMs": "0.0000", "470 Shutter-Volts": "0.0000", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "PRIME-PP 2 THRESHOLD": "125", "PRIME-PP 4 THRESHOLD": "75", "XYStage-Acceleration": "4.0000", "470 Shutter-Demo": "No", "PRIME-Name": "PMPCIECam00", "640-DA Device": "640 Shutter", "405 AO-Block voltage": "false", "ElapsedTime-ms": 1201205, "PRIME-Offset": "100", "445 AO-IOChannel": "Dev1/ao1", "640 AO-MaxVolts": "5.0000", "PositionIndex": 29, "PRIME-PP 1 ENABLED": "Yes", "COM1-BaudRate": "9600", "640-State": "0", "COM3-Verbose": "1", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "LudlController-TransmissionDelay": "0", "PRIME-FullWellCapacity": "62000", "PRIME-SMARTStreamingEnabled": "No", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "640 Shutter-Description": "NI DAC", "528-Description": "DA device that is used as a shutter", "COM4-Description": "Serial port driver (boost:asio)", "405 Shutter-Description": "NI DAC", "TE2000-Name": "TE2000", "528 Shutter-Description": "NI DAC", "COM9-Description": "Serial port driver (boost:asio)", "LudlController-Name": "LudlController", "445 Shutter-TriggerSequenceLength": "1000", "PRIME-SerialNumber": "A18B203008", "445 AO-Volts": "0.0000", "PRIME-X-dimension": "1200", "PRIME-ShutterCloseDelay": "0", "XLIGHT Disk slider-State": "2", "Position": "Default", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 AO-IOChannel": "Dev1/ao5", "520 AO-IOChannel": "Dev1/ao4", "528 AO-TriggerSequenceLength": "1000", "405 Shutter-MaxVolts": "5.0000", "470-DA Device": "470 Shutter", "PRIME-FTCapable": "No", "Core-ChannelGroup": "Channels", "555 AO-SupportsTriggering": "0", "PRIME-Y-dimension": "1200", "Frame": 0, "555 Shutter-Sequenceable": "1", "PRIME-Timing-ExposureTimeNs": "499998000.0000", "Core-ImageProcessor": "", "528-State": "0", "520 AO-Block voltage": "false", "XLIGHT Excitation Wheel-Label": "QUAD", "640 Shutter-Demo": "No", "COM3-StopBits": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "520 Shutter-IOChannel": "Dev1/ao11", "Width": 1200, "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-SupportsTriggering": "0", "640 AO-TriggerSampleRate": "100000", "520-Description": "DA device that is used as a shutter", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "405 Shutter-Sequenceable": "1", "405 Shutter-SupportsTriggering": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-TriggerSampleRate": "100000", "640 Shutter-MaxVolts": "5.0000", "555 AO-MaxVolts": "5.0000", "520 AO-TriggerSequenceLength": "1000", "COM3-BaudRate": "9600", "COM9-BaudRate": "9600", "Vincent-D3-Channel #": "Ch. #1", "PRIME-PMode": "Normal", "OpticalPath-Label": "Left(100)", "BitDepth": 16, "555 AO-TriggerSequenceLength": "1000", "445 Shutter-Description": "NI DAC", "405 AO-Sequenceable": "1", "PRIME-AcquisitionMethod": "Callbacks", "555 AO-Name": "AnalogIO", "PRIME-ShutterMode": "Pre-Exposure", "COM3-Handshaking": "Off", "445 AO-SupportsTriggering": "0", "ROI": "0-0-1200-1200", "COM9-Verbose": "1", "PRIME-Color": "OFF", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "PRIME-ShutterOpenDelay": "0", "COM1-Parity": "None", "Slice": 9, "SlicePosition": 40, "COM9-DelayBetweenCharsMs": "0.0000", "555-Name": "DA Shutter", "PIEZO-LudlSingleAxisName": "B", "XLIGHT Emission Wheel-Label": "585/11(T)", "640 Shutter-Name": "AnalogIO", "640 AO-Sequenceable": "1", "520 Shutter-Demo": "No", "528 Shutter-Demo": "No", "470-Name": "DA Shutter", "520-DA Device": "520 Shutter", "XYStage-Description": "Ludl XY stage driver adapter", "445 Shutter-MinVolts": "0.0000", "528 AO-TriggerSampleRate": "100000", "405 AO-MinVolts": "0.0000", "405 Shutter-TriggerSequenceLength": "1000", "Height": 1200, "555 AO-Block voltage": "false", "640 Shutter-Volts": "0.0000", "PixelType": "GRAY16", "445 Shutter-Block voltage": "false", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "520 Shutter-TriggerSequenceLength": "1000", "COM9-Name": "COM9", "Core-SLM": "", "555 AO-Volts": "4.5000", "555 Shutter-Block voltage": "false", "XYStage-Speed": "1800.0000", "520 AO-MaxVolts": "5.0000", "528 Shutter-SupportsTriggering": "0", "COM1-Description": "Serial port driver (boost:asio)", "PRIME-MetadataEnabled": "No", "520 Shutter-MinVolts": "0.0000", "470 Shutter-Sequenceable": "1", "PRIME-PreampDelay": "0", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-MinVolts": "0.0000", "ChannelIndex": 1, "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Core-AutoShutter": "1", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "Exposure-ms": 160, "PRIME-PP 2 ENABLED": "Yes", "640 Shutter-TriggerSampleRate": "100000", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "470 Shutter-IOChannel": "Dev1/ao10", "405 AO-Demo": "No", "PixelSizeUm": 1.1, "470 AO-Demo": "No", "470 Shutter-MaxVolts": "5.0000", "Vincent-D3-Name": "Vincent-D3", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FileName": "img_000000000_Il-T_009.tif", "LudlController-Reset": "Operate", "PRIME-Exposure": "160.00", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "445-Name": "DA Shutter", "555 Shutter-Name": "AnalogIO", "520 Shutter-TriggerSampleRate": "100000", "445 Shutter-IOChannel": "Dev1/ao9", "PRIME-TransposeCorrection": "0", "XYStage-StepSize": "0.1000", "445-Description": "DA device that is used as a shutter", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 AO-IOChannel": "Dev1/ao7", "520 Shutter-Name": "AnalogIO", "COM3-DataBits": "8", "640 AO-Volts": "0.0000", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "555 Shutter-IOChannel": "Dev1/ao13", "WaitInterval": null, "520 Shutter-Sequenceable": "1", "YPositionUm": -180, "445-State": "0", "445-DA Device": "445 Shutter", "PRIME-CircularBufferAutoSize": "ON", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "555 AO-TriggerSampleRate": "100000", "445 Shutter-Volts": "0.0000", "XLIGHT Hub-Port": "COM3", "Core-AutoFocus": "", "COM4-Handshaking": "Off", "528 Shutter-MinVolts": "0.0000", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "445 Shutter-Name": "AnalogIO", "528 Shutter-Sequenceable": "1", "XYStage-TransposeMirrorX": "0", "XYStage-TransposeMirrorY": "0", "PRIME-ReadoutRate": "100MHz 16bit", "XYStage-Name": "Stage", "640 AO-SupportsTriggering": "0", "640-Name": "DA Shutter", "640 Shutter-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "640 AO-Block voltage": "false", "445 Shutter-Sequenceable": "1", "470 AO-MaxVolts": "5.0000", "COM4-BaudRate": "9600", "470-Description": "DA device that is used as a shutter", "520 Shutter-Description": "NI DAC", "PRIME-CCDTemperature": "-14.8500", "Time": "2019-12-18 23:11:01 -0500", "640 AO-TriggerSequenceLength": "1000", "470 AO-Name": "AnalogIO", "XLIGHT Disk slider-Label": "Disk Pos 40um", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "Binning": "1x1", "TE2000-ModelType": "1", "COM3-AnswerTimeout": "500.0000", "555 Shutter-SupportsTriggering": "0", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "TE2000-Port": "COM4", "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Description": "NI DAC", "COM4-AnswerTimeout": "500.0000", "COM1-DelayBetweenCharsMs": "0.0000", "FilterBlock-Label": "Confocal", "445 AO-Name": "AnalogIO", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-Timing-ReadoutTimeNs": "24672000.0000", "528 AO-Demo": "No", "520 AO-Sequenceable": "1", "555-Description": "DA device that is used as a shutter", "405-DA Device": "405 Shutter", "405 AO-TriggerSequenceLength": "1000", "640 AO-MinVolts": "0.0000", "640 Shutter-Block voltage": "false", "FrameIndex": 0, "PRIME-Port": "CMOS", "555 AO-MinVolts": "0.0000", "COM3-Description": "Serial port driver (boost:asio)", "528 AO-Description": "NI DAC", "555 AO-IOChannel": "Dev1/ao6", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "555 AO-TriggerInputLine": "/Dev1/PFI0", "PRIME-ActualInterval-ms": "390.5200", "LudlController-Port": "COM9", "445 Shutter-Demo": "No", "PRIME-Trigger-Expose Out-Mux": "1", "SliceIndex": 9, "PRIME-CCDTemperatureSetPoint": "-15.0000", "405 Shutter-MinVolts": "0.0000", "PRIME-ExposeOutMode": "First Row", "AxisPositions": null, "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Volts": "5.0000", "528-Name": "DA Shutter", "555-State": "1", "XLIGHT Dichroic-Label": "QUAD", "445 AO-Sequenceable": "1", "405-Name": "DA Shutter", "405 AO-IOChannel": "Dev1/ao0", "Core-Camera": "PRIME", "Focus-Name": "Focus", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "405 Shutter-IOChannel": "Dev1/ao8", "640 AO-Demo": "No", "640 Shutter-SupportsTriggering": "0", "445 AO-Demo": "No", "555-DA Device": "555 Shutter", "470 Shutter-Description": "NI DAC", "528 AO-Volts": "0.0000", "COM1-DataBits": "8", "Vincent-D3-Address": "x", "520 AO-SupportsTriggering": "0", "405-Description": "DA device that is used as a shutter", "640 Shutter-MinVolts": "0.0000", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "405 AO-Name": "AnalogIO", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "640 AO-TriggerInputLine": "/Dev1/PFI0", "405 AO-TriggerInputLine": "/Dev1/PFI0", "640-Description": "DA device that is used as a shutter", "445 Shutter-TriggerSampleRate": "100000", "470 Shutter-TriggerSequenceLength": "1000", "COM1-StopBits": "1", "520 Shutter-SupportsTriggering": "0", "405-State": "0", "Core-Initialize": "1", "XLIGHT Emission Wheel-State": "3", "520 AO-Description": "NI DAC", "528 AO-TriggerInputLine": "/Dev1/PFI0", "445 Shutter-MaxVolts": "5.0000", "COM9-Handshaking": "Off", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "XLIGHT Spin motor-Label": "On (spinning)", "PRIME-PP 5 ENABLED": "No", "UUID": "b5fbdf88-c731-4f8b-84c8-7010c18a1015", "PRIME-TriggerMode": "Internal Trigger", "COM1-Handshaking": "Off", "PRIME-Timing-PostTriggerDelayNs": "49344000.0000", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "Focus-Position": "17741.5000", "528 Shutter-Block voltage": "false", "Channel": "Il-T", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "555 Shutter-Demo": "No", "405 Shutter-Demo": "No", "528 AO-Name": "AnalogIO", "520-State": "0", "Core-Galvo": "", "405 Shutter-TriggerSampleRate": "100000", "Focus-FocusStepSize_(nm)": "100", "PRIME-PixelType": "16bit", "COM4-DataBits": "8", "470 AO-IOChannel": "Dev1/ao2", "520 AO-MinVolts": "0.0000", "COM9-Parity": "None", "PRIME-CircularBufferEnabled": "ON", "PRIME-PP 6 Reset": "No", "640 AO-Description": "NI DAC", "Source": "PRIME", "445 AO-MinVolts": "0.0000", "PIEZO-Name": "Stage", "445 AO-Description": "NI DAC", "PRIME-CircularBufferFrameCount": "46", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "520 AO-Volts": "0.0000", "528 Shutter-TriggerSampleRate": "100000", "XPositionUm": 15712.1, "OpticalPath-State": "4", "PRIME-PP 3 THRESHOLD": "80", "PRIME-PVCAM Version": "3.7.1", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Gain": "1-HDR", "640 Shutter-TriggerSequenceLength": "1000", "528 AO-SupportsTriggering": "0", "445 AO-TriggerInputLine": "/Dev1/PFI0", "OpticalPath-Name": "OpticalPath", "PRIME-PP 4 ENABLED": "Yes", "PRIME-PVCAM Adapter Version": "1.3.29", "COM3-Name": "COM3", "405 AO-MaxVolts": "5.0000", "520 Shutter-Block voltage": "false", "PRIME-Timing-ClearingTimeNs": "49344000.0000", "Core-Focus": "PIEZO", "528 AO-Block voltage": "false", "TE2000-Description": "Nikon TE2000 hub", "528 AO-MinVolts": "0.0000", "COM4-Parity": "None", "Summary": { "Slices": 11, "ChContrastMin": [ 0, 0, 0, 0, 0, 0 ], "PixelAspect": 1, "SlicesFirst": true, "Interval_ms": 0, "Time": "2019-12-18 23:10:50 -0500", "Prefix": "seq_5", "InitialPositionList": [ { "Label": "Pos0", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.21], "Focus": [17741.5], "XYStage": [ 44542.200000000004, 31636.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos1", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.22], "Focus": [17741.4], "XYStage": [ 43801.100000000006, 31637.9 ] }, "GridColumnIndex": 0 }, { "Label": "Pos2", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.5], "XYStage": [ 38561.3, 32040.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos3", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.185], "Focus": [17741.3], "XYStage": [ 37698.4, 32041.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos4", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.16], "Focus": [17741.5], "XYStage": [ 32375.4, 31758.9 ] }, "GridColumnIndex": 0 }, { "Label": "Pos5", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.17000000000002], "Focus": [17741.3], "XYStage": [ 31770.7, 31758.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos6", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.4], "XYStage": [ 26536.800000000003, 31068.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos7", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.205], "Focus": [17741.4], "XYStage": [ 25420.2, 31068.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos8", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.85], "Focus": [17741.3], "XYStage": [ 21059, 31508 ] }, "GridColumnIndex": 0 }, { "Label": "Pos9", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.82], "Focus": [17741.3], "XYStage": [ 19731.9, 31506.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos10", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.79], "Focus": [17741.4], "XYStage": [ 14528.5, 32007.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos11", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.83], "Focus": [17741.2], "XYStage": [ 13830.300000000001, 32009.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos12", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.79], "Focus": [17741.3], "XYStage": [ 8775.300000000001, 32017.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos13", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.815], "Focus": [17741.3], "XYStage": [ 8109.200000000001, 32008.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos14", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.805], "Focus": [17741.3], "XYStage": [ 3483.3, 31908.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos15", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.82], "Focus": [17741.2], "XYStage": [ 2686.8, 31903.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos16", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [211.48000000000002], "Focus": [17741.1], "XYStage": [ -1978.9, 31973.600000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos17", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [211.51500000000001], "Focus": [17741.1], "XYStage": [ -2957.8, 31982.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos18", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.195], "Focus": [17741.5], "XYStage": [ 47747.4, -576.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos19", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.23000000000002], "Focus": [17741.6], "XYStage": [ 47053.9, -576.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos20", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.205], "Focus": [17741.4], "XYStage": [ 41871.200000000004, -479.8 ] }, "GridColumnIndex": 0 }, { "Label": "Pos21", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.4], "XYStage": [ 40882.600000000006, -392 ] }, "GridColumnIndex": 0 }, { "Label": "Pos22", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.24], "Focus": [17741.4], "XYStage": [ 35905.6, -135.3 ] }, "GridColumnIndex": 0 }, { "Label": "Pos23", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.195], "Focus": [17741.5], "XYStage": [ 34990.9, -132.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos24", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.46], "Focus": [17741.4], "XYStage": [ 29678.100000000002, -958.1 ] }, "GridColumnIndex": 0 }, { "Label": "Pos25", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.44], "Focus": [17741.5], "XYStage": [ 28983.600000000002, -939.4000000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos26", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.475], "Focus": [17741.5], "XYStage": [ 23019.7, -122 ] }, "GridColumnIndex": 0 }, { "Label": "Pos27", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.44], "Focus": [17741.7], "XYStage": [ 22428, -116.80000000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos28", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.46], "Focus": [17741.7], "XYStage": [ 16364.900000000001, -181.10000000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos29", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.48000000000002], "Focus": [17741.5], "XYStage": [ 15712.1, -180 ] }, "GridColumnIndex": 0 }, { "Label": "Pos30", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.45000000000002], "Focus": [17741.7], "XYStage": [ 10346.1, 77.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos31", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.49], "Focus": [17741.4], "XYStage": [ 9755.5, 85.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos32", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.475], "Focus": [17741.5], "XYStage": [ 4775.5, 293.90000000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos33", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.445], "Focus": [17741.4], "XYStage": [ 3954.9, 293.90000000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos34", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.455], "Focus": [17741.4], "XYStage": [ -1324.9, 545.6 ] }, "GridColumnIndex": 0 }, { "Label": "Pos35", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.445], "Focus": [17741.3], "XYStage": [ -2097, 576.6 ] }, "GridColumnIndex": 0 } ], "z-step_um": 10, "GridRow": 0, "BitDepth": 16, "Depth": 2, "Channels": 6, "Source": "Micro-Manager", "ChColors": [ -16711681, -256, -65281, -1, -1, -16776961 ], "MetadataVersion": 10, "KeepShutterOpenChannels": true, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536, 65536 ], "CustomIntervals_ms": [], "Height": 1200, "MicroManagerVersion": "1.4.23 20180219", "GridColumn": 0, "UUID": "67598d21-5db5-4842-ab60-f506f7b42c7c", "PixelType": "GRAY16", "Width": 1200, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\Yan\\20191218-YC113-S7S8-miseq", "Frames": 1, "Comment": "", "UserName": "Nikon", "PixelSize_um": 1.1, "ROI": [ 0, 0, 1200, 1200 ], "Date": "2019-12-18", "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC", "P-DAPI-high" ], "KeepShutterOpenSlices": true, "IJType": 1, "ComputerName": "ZadorConfocal2", "Positions": 36, "PositionIndex": 29, "CameraTimeout": 20000, "TimeFirst": false }, "640 AO-Name": "AnalogIO", "Vincent-D3-Command": "Close" }, "FrameKey-0-1-10": { "COM4-StopBits": "1", "528 AO-MaxVolts": "5.0000", "XLIGHT Excitation Wheel-State": "1", "470 Shutter-Name": "AnalogIO", "Core-Shutter": "555", "555 AO-Demo": "No", "XLIGHT Dichroic-State": "2", "XYStage-StepSize-Y": "0.1000", "ZPositionUm": 268.48, "XYStage-StepSize-X": "0.1000", "PRIME-FirmwareVersion": "20.28", "PRIME-ClearCycles": "2", "520-Name": "DA Shutter", "405 Shutter-Name": "AnalogIO", "PIEZO-StepSize": "0.0050", "PRIME-ClearMode": "Pre-Exposure", "470 AO-MinVolts": "0.0000", "405 AO-SupportsTriggering": "1", "CameraChannelIndex": 0, "XYStage-StartSpeed": "500.0000", "COM9-StopBits": "2", "405 AO-Description": "NI DAC", "COM4-DelayBetweenCharsMs": "0.0000", "445 AO-Block voltage": "false", "520 AO-TriggerInputLine": "/Dev1/PFI0", "555 AO-Description": "NI DAC", "520 AO-TriggerSampleRate": "100000", "COM4-Verbose": "1", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM4-Name": "COM4", "405 Shutter-Volts": "0.0000", "Vincent-D3-Port": "COM1", "PRIME-PP 4 MIN ADU AFFECTED": "200", "470 Shutter-MinVolts": "0.0000", "PRIME-PP 3 ENABLED": "Yes", "470 AO-Description": "NI DAC", "PRIME-Description": "PVCAM API device adapter", "520 Shutter-MaxVolts": "5.0000", "520 Shutter-Volts": "0.0000", "528 AO-Sequenceable": "1", "PRIME-Binning": "1x1", "555 Shutter-MaxVolts": "5.0000", "528 Shutter-TriggerSequenceLength": "1000", "405 Shutter-Block voltage": "false", "XLIGHT Spin motor-State": "1", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "Vincent-D3-Time to open (ms)": "35.0000", "Vincent-D3-Time to close (ms)": "35.0000", "445 AO-TriggerSampleRate": "100000", "640 Shutter-IOChannel": "Dev1/ao14", "555 AO-Sequenceable": "1", "520 AO-Name": "AnalogIO", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "470 AO-Block voltage": "false", "COM1-Verbose": "1", "COM9-AnswerTimeout": "10000.0000", "528 Shutter-Name": "AnalogIO", "PRIME-Trigger Timeout (secs)": "10", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "NextFrame": 0, "470 AO-TriggerSequenceLength": "1000", "PRIME-TransposeXY": "0", "445 AO-TriggerSequenceLength": "1000", "COM1-Name": "COM1", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "COM9-DataBits": "8", "COM1-AnswerTimeout": "500.0000", "Camera": "", "TE2000-Version": "V2.50-V2.50-V2.10", "528 Shutter-Volts": "0.0000", "XLIGHT Hub-Name": "XLIGHT Hub", "PIEZO-Description": "Ludl stage driver adapter", "PositionName": "Pos29", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSampleRate": "100000", "PRIME-FanSpeedSetpoint": "High", "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "405 AO-TriggerSampleRate": "100000", "470 Shutter-Block voltage": "false", "470-State": "0", "COM3-Parity": "None", "405 AO-Volts": "0.0000", "PRIME-TransposeMirrorY": "0", "PRIME-ChipName": "GS144BSI", "470 AO-Volts": "0.0000", "PRIME-TransposeMirrorX": "0", "445 Shutter-SupportsTriggering": "0", "470 Shutter-SupportsTriggering": "0", "XLIGHT Hub-Description": "XLIGHT hub", "PRIME-PreampOffLimit": "10000", "470 AO-Sequenceable": "1", "528 Shutter-MaxVolts": "5.0000", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "Core-XYStage": "XYStage", "470 AO-TriggerSampleRate": "100000", "PRIME-Timing-PreTriggerDelayNs": "24672000.0000", "528-DA Device": "528 Shutter", "Focus-Description": "Nikon TE2000 focus adapter", "FilterBlock-State": "5", "520 AO-Demo": "No", "COM3-DelayBetweenCharsMs": "0.0000", "470 Shutter-Volts": "0.0000", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "PRIME-PP 2 THRESHOLD": "125", "PRIME-PP 4 THRESHOLD": "75", "XYStage-Acceleration": "4.0000", "470 Shutter-Demo": "No", "PRIME-Name": "PMPCIECam00", "640-DA Device": "640 Shutter", "405 AO-Block voltage": "false", "ElapsedTime-ms": 1201605, "PRIME-Offset": "100", "445 AO-IOChannel": "Dev1/ao1", "640 AO-MaxVolts": "5.0000", "PositionIndex": 29, "PRIME-PP 1 ENABLED": "Yes", "COM1-BaudRate": "9600", "640-State": "0", "COM3-Verbose": "1", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "LudlController-TransmissionDelay": "0", "PRIME-FullWellCapacity": "62000", "PRIME-SMARTStreamingEnabled": "No", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "640 Shutter-Description": "NI DAC", "528-Description": "DA device that is used as a shutter", "COM4-Description": "Serial port driver (boost:asio)", "405 Shutter-Description": "NI DAC", "TE2000-Name": "TE2000", "528 Shutter-Description": "NI DAC", "COM9-Description": "Serial port driver (boost:asio)", "LudlController-Name": "LudlController", "445 Shutter-TriggerSequenceLength": "1000", "PRIME-SerialNumber": "A18B203008", "445 AO-Volts": "0.0000", "PRIME-X-dimension": "1200", "PRIME-ShutterCloseDelay": "0", "XLIGHT Disk slider-State": "2", "Position": "Default", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 AO-IOChannel": "Dev1/ao5", "520 AO-IOChannel": "Dev1/ao4", "528 AO-TriggerSequenceLength": "1000", "405 Shutter-MaxVolts": "5.0000", "470-DA Device": "470 Shutter", "PRIME-FTCapable": "No", "Core-ChannelGroup": "Channels", "555 AO-SupportsTriggering": "0", "PRIME-Y-dimension": "1200", "Frame": 0, "555 Shutter-Sequenceable": "1", "PRIME-Timing-ExposureTimeNs": "499998000.0000", "Core-ImageProcessor": "", "528-State": "0", "520 AO-Block voltage": "false", "XLIGHT Excitation Wheel-Label": "QUAD", "640 Shutter-Demo": "No", "COM3-StopBits": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "520 Shutter-IOChannel": "Dev1/ao11", "Width": 1200, "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-SupportsTriggering": "0", "640 AO-TriggerSampleRate": "100000", "520-Description": "DA device that is used as a shutter", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "405 Shutter-Sequenceable": "1", "405 Shutter-SupportsTriggering": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-TriggerSampleRate": "100000", "640 Shutter-MaxVolts": "5.0000", "555 AO-MaxVolts": "5.0000", "520 AO-TriggerSequenceLength": "1000", "COM3-BaudRate": "9600", "COM9-BaudRate": "9600", "Vincent-D3-Channel #": "Ch. #1", "PRIME-PMode": "Normal", "OpticalPath-Label": "Left(100)", "BitDepth": 16, "555 AO-TriggerSequenceLength": "1000", "445 Shutter-Description": "NI DAC", "405 AO-Sequenceable": "1", "PRIME-AcquisitionMethod": "Callbacks", "555 AO-Name": "AnalogIO", "PRIME-ShutterMode": "Pre-Exposure", "COM3-Handshaking": "Off", "445 AO-SupportsTriggering": "0", "ROI": "0-0-1200-1200", "COM9-Verbose": "1", "PRIME-Color": "OFF", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "PRIME-ShutterOpenDelay": "0", "COM1-Parity": "None", "Slice": 10, "SlicePosition": 50, "COM9-DelayBetweenCharsMs": "0.0000", "555-Name": "DA Shutter", "PIEZO-LudlSingleAxisName": "B", "XLIGHT Emission Wheel-Label": "585/11(T)", "640 Shutter-Name": "AnalogIO", "640 AO-Sequenceable": "1", "520 Shutter-Demo": "No", "528 Shutter-Demo": "No", "470-Name": "DA Shutter", "520-DA Device": "520 Shutter", "XYStage-Description": "Ludl XY stage driver adapter", "445 Shutter-MinVolts": "0.0000", "528 AO-TriggerSampleRate": "100000", "405 AO-MinVolts": "0.0000", "405 Shutter-TriggerSequenceLength": "1000", "Height": 1200, "555 AO-Block voltage": "false", "640 Shutter-Volts": "0.0000", "PixelType": "GRAY16", "445 Shutter-Block voltage": "false", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "520 Shutter-TriggerSequenceLength": "1000", "COM9-Name": "COM9", "Core-SLM": "", "555 AO-Volts": "4.5000", "555 Shutter-Block voltage": "false", "XYStage-Speed": "1800.0000", "520 AO-MaxVolts": "5.0000", "528 Shutter-SupportsTriggering": "0", "COM1-Description": "Serial port driver (boost:asio)", "PRIME-MetadataEnabled": "No", "520 Shutter-MinVolts": "0.0000", "470 Shutter-Sequenceable": "1", "PRIME-PreampDelay": "0", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-MinVolts": "0.0000", "ChannelIndex": 1, "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Core-AutoShutter": "1", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "Exposure-ms": 160, "PRIME-PP 2 ENABLED": "Yes", "640 Shutter-TriggerSampleRate": "100000", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "470 Shutter-IOChannel": "Dev1/ao10", "405 AO-Demo": "No", "PixelSizeUm": 1.1, "470 AO-Demo": "No", "470 Shutter-MaxVolts": "5.0000", "Vincent-D3-Name": "Vincent-D3", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FileName": "img_000000000_Il-T_010.tif", "LudlController-Reset": "Operate", "PRIME-Exposure": "160.00", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "445-Name": "DA Shutter", "555 Shutter-Name": "AnalogIO", "520 Shutter-TriggerSampleRate": "100000", "445 Shutter-IOChannel": "Dev1/ao9", "PRIME-TransposeCorrection": "0", "XYStage-StepSize": "0.1000", "445-Description": "DA device that is used as a shutter", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 AO-IOChannel": "Dev1/ao7", "520 Shutter-Name": "AnalogIO", "COM3-DataBits": "8", "640 AO-Volts": "0.0000", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "555 Shutter-IOChannel": "Dev1/ao13", "WaitInterval": null, "520 Shutter-Sequenceable": "1", "YPositionUm": -180, "445-State": "0", "445-DA Device": "445 Shutter", "PRIME-CircularBufferAutoSize": "ON", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "555 AO-TriggerSampleRate": "100000", "445 Shutter-Volts": "0.0000", "XLIGHT Hub-Port": "COM3", "Core-AutoFocus": "", "COM4-Handshaking": "Off", "528 Shutter-MinVolts": "0.0000", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "445 Shutter-Name": "AnalogIO", "528 Shutter-Sequenceable": "1", "XYStage-TransposeMirrorX": "0", "XYStage-TransposeMirrorY": "0", "PRIME-ReadoutRate": "100MHz 16bit", "XYStage-Name": "Stage", "640 AO-SupportsTriggering": "0", "640-Name": "DA Shutter", "640 Shutter-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "640 AO-Block voltage": "false", "445 Shutter-Sequenceable": "1", "470 AO-MaxVolts": "5.0000", "COM4-BaudRate": "9600", "470-Description": "DA device that is used as a shutter", "520 Shutter-Description": "NI DAC", "PRIME-CCDTemperature": "-14.8500", "Time": "2019-12-18 23:11:01 -0500", "640 AO-TriggerSequenceLength": "1000", "470 AO-Name": "AnalogIO", "XLIGHT Disk slider-Label": "Disk Pos 40um", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "Binning": "1x1", "TE2000-ModelType": "1", "COM3-AnswerTimeout": "500.0000", "555 Shutter-SupportsTriggering": "0", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "TE2000-Port": "COM4", "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Description": "NI DAC", "COM4-AnswerTimeout": "500.0000", "COM1-DelayBetweenCharsMs": "0.0000", "FilterBlock-Label": "Confocal", "445 AO-Name": "AnalogIO", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-Timing-ReadoutTimeNs": "24672000.0000", "528 AO-Demo": "No", "520 AO-Sequenceable": "1", "555-Description": "DA device that is used as a shutter", "405-DA Device": "405 Shutter", "405 AO-TriggerSequenceLength": "1000", "640 AO-MinVolts": "0.0000", "640 Shutter-Block voltage": "false", "FrameIndex": 0, "PRIME-Port": "CMOS", "555 AO-MinVolts": "0.0000", "COM3-Description": "Serial port driver (boost:asio)", "528 AO-Description": "NI DAC", "555 AO-IOChannel": "Dev1/ao6", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "555 AO-TriggerInputLine": "/Dev1/PFI0", "PRIME-ActualInterval-ms": "390.5200", "LudlController-Port": "COM9", "445 Shutter-Demo": "No", "PRIME-Trigger-Expose Out-Mux": "1", "SliceIndex": 10, "PRIME-CCDTemperatureSetPoint": "-15.0000", "405 Shutter-MinVolts": "0.0000", "PRIME-ExposeOutMode": "First Row", "AxisPositions": null, "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Volts": "5.0000", "528-Name": "DA Shutter", "555-State": "0", "XLIGHT Dichroic-Label": "QUAD", "445 AO-Sequenceable": "1", "405-Name": "DA Shutter", "405 AO-IOChannel": "Dev1/ao0", "Core-Camera": "PRIME", "Focus-Name": "Focus", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "405 Shutter-IOChannel": "Dev1/ao8", "640 AO-Demo": "No", "640 Shutter-SupportsTriggering": "0", "445 AO-Demo": "No", "555-DA Device": "555 Shutter", "470 Shutter-Description": "NI DAC", "528 AO-Volts": "0.0000", "COM1-DataBits": "8", "Vincent-D3-Address": "x", "520 AO-SupportsTriggering": "0", "405-Description": "DA device that is used as a shutter", "640 Shutter-MinVolts": "0.0000", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "405 AO-Name": "AnalogIO", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "640 AO-TriggerInputLine": "/Dev1/PFI0", "405 AO-TriggerInputLine": "/Dev1/PFI0", "640-Description": "DA device that is used as a shutter", "445 Shutter-TriggerSampleRate": "100000", "470 Shutter-TriggerSequenceLength": "1000", "COM1-StopBits": "1", "520 Shutter-SupportsTriggering": "0", "405-State": "0", "Core-Initialize": "1", "XLIGHT Emission Wheel-State": "3", "520 AO-Description": "NI DAC", "528 AO-TriggerInputLine": "/Dev1/PFI0", "445 Shutter-MaxVolts": "5.0000", "COM9-Handshaking": "Off", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "XLIGHT Spin motor-Label": "On (spinning)", "PRIME-PP 5 ENABLED": "No", "UUID": "7b4e53ce-2c1f-4d49-8087-a4ef286ba245", "PRIME-TriggerMode": "Internal Trigger", "COM1-Handshaking": "Off", "PRIME-Timing-PostTriggerDelayNs": "49344000.0000", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "Focus-Position": "17741.5000", "528 Shutter-Block voltage": "false", "Channel": "Il-T", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "555 Shutter-Demo": "No", "405 Shutter-Demo": "No", "528 AO-Name": "AnalogIO", "520-State": "0", "Core-Galvo": "", "405 Shutter-TriggerSampleRate": "100000", "Focus-FocusStepSize_(nm)": "100", "PRIME-PixelType": "16bit", "COM4-DataBits": "8", "470 AO-IOChannel": "Dev1/ao2", "520 AO-MinVolts": "0.0000", "COM9-Parity": "None", "PRIME-CircularBufferEnabled": "ON", "PRIME-PP 6 Reset": "No", "640 AO-Description": "NI DAC", "Source": "PRIME", "445 AO-MinVolts": "0.0000", "PIEZO-Name": "Stage", "445 AO-Description": "NI DAC", "PRIME-CircularBufferFrameCount": "46", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "520 AO-Volts": "0.0000", "528 Shutter-TriggerSampleRate": "100000", "XPositionUm": 15712.1, "OpticalPath-State": "4", "PRIME-PP 3 THRESHOLD": "80", "PRIME-PVCAM Version": "3.7.1", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Gain": "1-HDR", "640 Shutter-TriggerSequenceLength": "1000", "528 AO-SupportsTriggering": "0", "445 AO-TriggerInputLine": "/Dev1/PFI0", "OpticalPath-Name": "OpticalPath", "PRIME-PP 4 ENABLED": "Yes", "PRIME-PVCAM Adapter Version": "1.3.29", "COM3-Name": "COM3", "405 AO-MaxVolts": "5.0000", "520 Shutter-Block voltage": "false", "PRIME-Timing-ClearingTimeNs": "49344000.0000", "Core-Focus": "PIEZO", "528 AO-Block voltage": "false", "TE2000-Description": "Nikon TE2000 hub", "528 AO-MinVolts": "0.0000", "COM4-Parity": "None", "Summary": { "Slices": 11, "ChContrastMin": [ 0, 0, 0, 0, 0, 0 ], "PixelAspect": 1, "SlicesFirst": true, "Interval_ms": 0, "Time": "2019-12-18 23:10:50 -0500", "Prefix": "seq_5", "InitialPositionList": [ { "Label": "Pos0", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.21], "Focus": [17741.5], "XYStage": [ 44542.200000000004, 31636.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos1", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.22], "Focus": [17741.4], "XYStage": [ 43801.100000000006, 31637.9 ] }, "GridColumnIndex": 0 }, { "Label": "Pos2", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.5], "XYStage": [ 38561.3, 32040.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos3", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.185], "Focus": [17741.3], "XYStage": [ 37698.4, 32041.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos4", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.16], "Focus": [17741.5], "XYStage": [ 32375.4, 31758.9 ] }, "GridColumnIndex": 0 }, { "Label": "Pos5", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.17000000000002], "Focus": [17741.3], "XYStage": [ 31770.7, 31758.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos6", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.4], "XYStage": [ 26536.800000000003, 31068.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos7", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.205], "Focus": [17741.4], "XYStage": [ 25420.2, 31068.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos8", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.85], "Focus": [17741.3], "XYStage": [ 21059, 31508 ] }, "GridColumnIndex": 0 }, { "Label": "Pos9", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.82], "Focus": [17741.3], "XYStage": [ 19731.9, 31506.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos10", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.79], "Focus": [17741.4], "XYStage": [ 14528.5, 32007.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos11", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.83], "Focus": [17741.2], "XYStage": [ 13830.300000000001, 32009.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos12", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.79], "Focus": [17741.3], "XYStage": [ 8775.300000000001, 32017.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos13", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.815], "Focus": [17741.3], "XYStage": [ 8109.200000000001, 32008.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos14", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.805], "Focus": [17741.3], "XYStage": [ 3483.3, 31908.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos15", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.82], "Focus": [17741.2], "XYStage": [ 2686.8, 31903.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos16", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [211.48000000000002], "Focus": [17741.1], "XYStage": [ -1978.9, 31973.600000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos17", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [211.51500000000001], "Focus": [17741.1], "XYStage": [ -2957.8, 31982.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos18", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.195], "Focus": [17741.5], "XYStage": [ 47747.4, -576.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos19", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.23000000000002], "Focus": [17741.6], "XYStage": [ 47053.9, -576.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos20", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.205], "Focus": [17741.4], "XYStage": [ 41871.200000000004, -479.8 ] }, "GridColumnIndex": 0 }, { "Label": "Pos21", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.4], "XYStage": [ 40882.600000000006, -392 ] }, "GridColumnIndex": 0 }, { "Label": "Pos22", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.24], "Focus": [17741.4], "XYStage": [ 35905.6, -135.3 ] }, "GridColumnIndex": 0 }, { "Label": "Pos23", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.195], "Focus": [17741.5], "XYStage": [ 34990.9, -132.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos24", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.46], "Focus": [17741.4], "XYStage": [ 29678.100000000002, -958.1 ] }, "GridColumnIndex": 0 }, { "Label": "Pos25", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.44], "Focus": [17741.5], "XYStage": [ 28983.600000000002, -939.4000000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos26", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.475], "Focus": [17741.5], "XYStage": [ 23019.7, -122 ] }, "GridColumnIndex": 0 }, { "Label": "Pos27", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.44], "Focus": [17741.7], "XYStage": [ 22428, -116.80000000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos28", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.46], "Focus": [17741.7], "XYStage": [ 16364.900000000001, -181.10000000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos29", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.48000000000002], "Focus": [17741.5], "XYStage": [ 15712.1, -180 ] }, "GridColumnIndex": 0 }, { "Label": "Pos30", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.45000000000002], "Focus": [17741.7], "XYStage": [ 10346.1, 77.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos31", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.49], "Focus": [17741.4], "XYStage": [ 9755.5, 85.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos32", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.475], "Focus": [17741.5], "XYStage": [ 4775.5, 293.90000000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos33", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.445], "Focus": [17741.4], "XYStage": [ 3954.9, 293.90000000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos34", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.455], "Focus": [17741.4], "XYStage": [ -1324.9, 545.6 ] }, "GridColumnIndex": 0 }, { "Label": "Pos35", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.445], "Focus": [17741.3], "XYStage": [ -2097, 576.6 ] }, "GridColumnIndex": 0 } ], "z-step_um": 10, "GridRow": 0, "BitDepth": 16, "Depth": 2, "Channels": 6, "Source": "Micro-Manager", "ChColors": [ -16711681, -256, -65281, -1, -1, -16776961 ], "MetadataVersion": 10, "KeepShutterOpenChannels": true, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536, 65536 ], "CustomIntervals_ms": [], "Height": 1200, "MicroManagerVersion": "1.4.23 20180219", "GridColumn": 0, "UUID": "67598d21-5db5-4842-ab60-f506f7b42c7c", "PixelType": "GRAY16", "Width": 1200, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\Yan\\20191218-YC113-S7S8-miseq", "Frames": 1, "Comment": "", "UserName": "Nikon", "PixelSize_um": 1.1, "ROI": [ 0, 0, 1200, 1200 ], "Date": "2019-12-18", "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC", "P-DAPI-high" ], "KeepShutterOpenSlices": true, "IJType": 1, "ComputerName": "ZadorConfocal2", "Positions": 36, "PositionIndex": 29, "CameraTimeout": 20000, "TimeFirst": false }, "640 AO-Name": "AnalogIO", "Vincent-D3-Command": "Close" }, "FrameKey-0-2-0": { "COM4-StopBits": "1", "528 AO-MaxVolts": "5.0000", "XLIGHT Excitation Wheel-State": "1", "470 Shutter-Name": "AnalogIO", "Core-Shutter": "640", "555 AO-Demo": "No", "XLIGHT Dichroic-State": "2", "XYStage-StepSize-Y": "0.1000", "ZPositionUm": 168.48000000000002, "XYStage-StepSize-X": "0.1000", "PRIME-FirmwareVersion": "20.28", "PRIME-ClearCycles": "2", "520-Name": "DA Shutter", "405 Shutter-Name": "AnalogIO", "PIEZO-StepSize": "0.0050", "PRIME-ClearMode": "Pre-Exposure", "470 AO-MinVolts": "0.0000", "405 AO-SupportsTriggering": "1", "CameraChannelIndex": 0, "XYStage-StartSpeed": "500.0000", "COM9-StopBits": "2", "405 AO-Description": "NI DAC", "COM4-DelayBetweenCharsMs": "0.0000", "445 AO-Block voltage": "false", "520 AO-TriggerInputLine": "/Dev1/PFI0", "555 AO-Description": "NI DAC", "520 AO-TriggerSampleRate": "100000", "COM4-Verbose": "1", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM4-Name": "COM4", "405 Shutter-Volts": "0.0000", "Vincent-D3-Port": "COM1", "PRIME-PP 4 MIN ADU AFFECTED": "200", "470 Shutter-MinVolts": "0.0000", "PRIME-PP 3 ENABLED": "Yes", "470 AO-Description": "NI DAC", "PRIME-Description": "PVCAM API device adapter", "520 Shutter-MaxVolts": "5.0000", "520 Shutter-Volts": "0.0000", "528 AO-Sequenceable": "1", "PRIME-Binning": "1x1", "555 Shutter-MaxVolts": "5.0000", "528 Shutter-TriggerSequenceLength": "1000", "405 Shutter-Block voltage": "false", "XLIGHT Spin motor-State": "1", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "Vincent-D3-Time to open (ms)": "35.0000", "Vincent-D3-Time to close (ms)": "35.0000", "445 AO-TriggerSampleRate": "100000", "640 Shutter-IOChannel": "Dev1/ao14", "555 AO-Sequenceable": "1", "520 AO-Name": "AnalogIO", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "470 AO-Block voltage": "false", "COM1-Verbose": "1", "COM9-AnswerTimeout": "10000.0000", "528 Shutter-Name": "AnalogIO", "PRIME-Trigger Timeout (secs)": "10", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "NextFrame": 0, "470 AO-TriggerSequenceLength": "1000", "PRIME-TransposeXY": "0", "445 AO-TriggerSequenceLength": "1000", "COM1-Name": "COM1", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "COM9-DataBits": "8", "COM1-AnswerTimeout": "500.0000", "Camera": "", "TE2000-Version": "V2.50-V2.50-V2.10", "528 Shutter-Volts": "0.0000", "XLIGHT Hub-Name": "XLIGHT Hub", "PIEZO-Description": "Ludl stage driver adapter", "PositionName": "Pos29", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSampleRate": "100000", "PRIME-FanSpeedSetpoint": "High", "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "405 AO-TriggerSampleRate": "100000", "470 Shutter-Block voltage": "false", "470-State": "0", "COM3-Parity": "None", "405 AO-Volts": "0.0000", "PRIME-TransposeMirrorY": "0", "PRIME-ChipName": "GS144BSI", "470 AO-Volts": "0.0000", "PRIME-TransposeMirrorX": "0", "445 Shutter-SupportsTriggering": "0", "470 Shutter-SupportsTriggering": "0", "XLIGHT Hub-Description": "XLIGHT hub", "PRIME-PreampOffLimit": "10000", "470 AO-Sequenceable": "1", "528 Shutter-MaxVolts": "5.0000", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "Core-XYStage": "XYStage", "470 AO-TriggerSampleRate": "100000", "PRIME-Timing-PreTriggerDelayNs": "24672000.0000", "528-DA Device": "528 Shutter", "Focus-Description": "Nikon TE2000 focus adapter", "FilterBlock-State": "5", "520 AO-Demo": "No", "COM3-DelayBetweenCharsMs": "0.0000", "470 Shutter-Volts": "0.0000", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "PRIME-PP 2 THRESHOLD": "125", "PRIME-PP 4 THRESHOLD": "75", "XYStage-Acceleration": "4.0000", "470 Shutter-Demo": "No", "PRIME-Name": "PMPCIECam00", "640-DA Device": "640 Shutter", "405 AO-Block voltage": "false", "ElapsedTime-ms": 1202778, "PRIME-Offset": "100", "445 AO-IOChannel": "Dev1/ao1", "640 AO-MaxVolts": "5.0000", "PositionIndex": 29, "PRIME-PP 1 ENABLED": "Yes", "COM1-BaudRate": "9600", "640-State": "1", "COM3-Verbose": "1", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "LudlController-TransmissionDelay": "0", "PRIME-FullWellCapacity": "62000", "PRIME-SMARTStreamingEnabled": "No", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "640 Shutter-Description": "NI DAC", "528-Description": "DA device that is used as a shutter", "COM4-Description": "Serial port driver (boost:asio)", "405 Shutter-Description": "NI DAC", "TE2000-Name": "TE2000", "528 Shutter-Description": "NI DAC", "COM9-Description": "Serial port driver (boost:asio)", "LudlController-Name": "LudlController", "445 Shutter-TriggerSequenceLength": "1000", "PRIME-SerialNumber": "A18B203008", "445 AO-Volts": "0.0000", "PRIME-X-dimension": "1200", "PRIME-ShutterCloseDelay": "0", "XLIGHT Disk slider-State": "2", "Position": "Default", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 AO-IOChannel": "Dev1/ao5", "520 AO-IOChannel": "Dev1/ao4", "528 AO-TriggerSequenceLength": "1000", "405 Shutter-MaxVolts": "5.0000", "470-DA Device": "470 Shutter", "PRIME-FTCapable": "No", "Core-ChannelGroup": "Channels", "555 AO-SupportsTriggering": "0", "PRIME-Y-dimension": "1200", "Frame": 0, "555 Shutter-Sequenceable": "1", "PRIME-Timing-ExposureTimeNs": "499998000.0000", "Core-ImageProcessor": "", "528-State": "0", "520 AO-Block voltage": "false", "XLIGHT Excitation Wheel-Label": "QUAD", "640 Shutter-Demo": "No", "COM3-StopBits": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "520 Shutter-IOChannel": "Dev1/ao11", "Width": 1200, "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-SupportsTriggering": "0", "640 AO-TriggerSampleRate": "100000", "520-Description": "DA device that is used as a shutter", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "405 Shutter-Sequenceable": "1", "405 Shutter-SupportsTriggering": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-TriggerSampleRate": "100000", "640 Shutter-MaxVolts": "5.0000", "555 AO-MaxVolts": "5.0000", "520 AO-TriggerSequenceLength": "1000", "COM3-BaudRate": "9600", "COM9-BaudRate": "9600", "Vincent-D3-Channel #": "Ch. #1", "PRIME-PMode": "Normal", "OpticalPath-Label": "Left(100)", "BitDepth": 16, "555 AO-TriggerSequenceLength": "1000", "445 Shutter-Description": "NI DAC", "405 AO-Sequenceable": "1", "PRIME-AcquisitionMethod": "Callbacks", "555 AO-Name": "AnalogIO", "PRIME-ShutterMode": "Pre-Exposure", "COM3-Handshaking": "Off", "445 AO-SupportsTriggering": "0", "ROI": "0-0-1200-1200", "COM9-Verbose": "1", "PRIME-Color": "OFF", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "PRIME-ShutterOpenDelay": "0", "COM1-Parity": "None", "Slice": 0, "SlicePosition": -50, "COM9-DelayBetweenCharsMs": "0.0000", "555-Name": "DA Shutter", "PIEZO-LudlSingleAxisName": "B", "XLIGHT Emission Wheel-Label": "679/29 (A)", "640 Shutter-Name": "AnalogIO", "640 AO-Sequenceable": "1", "520 Shutter-Demo": "No", "528 Shutter-Demo": "No", "470-Name": "DA Shutter", "520-DA Device": "520 Shutter", "XYStage-Description": "Ludl XY stage driver adapter", "445 Shutter-MinVolts": "0.0000", "528 AO-TriggerSampleRate": "100000", "405 AO-MinVolts": "0.0000", "405 Shutter-TriggerSequenceLength": "1000", "Height": 1200, "555 AO-Block voltage": "false", "640 Shutter-Volts": "5.0000", "PixelType": "GRAY16", "445 Shutter-Block voltage": "false", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "520 Shutter-TriggerSequenceLength": "1000", "COM9-Name": "COM9", "Core-SLM": "", "555 AO-Volts": "0.0000", "555 Shutter-Block voltage": "false", "XYStage-Speed": "1800.0000", "520 AO-MaxVolts": "5.0000", "528 Shutter-SupportsTriggering": "0", "COM1-Description": "Serial port driver (boost:asio)", "PRIME-MetadataEnabled": "No", "520 Shutter-MinVolts": "0.0000", "470 Shutter-Sequenceable": "1", "PRIME-PreampDelay": "0", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-MinVolts": "0.0000", "ChannelIndex": 2, "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Core-AutoShutter": "1", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "Exposure-ms": 320, "PRIME-PP 2 ENABLED": "Yes", "640 Shutter-TriggerSampleRate": "100000", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "470 Shutter-IOChannel": "Dev1/ao10", "405 AO-Demo": "No", "PixelSizeUm": 1.1, "470 AO-Demo": "No", "470 Shutter-MaxVolts": "5.0000", "Vincent-D3-Name": "Vincent-D3", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FileName": "img_000000000_Il-A_000.tif", "LudlController-Reset": "Operate", "PRIME-Exposure": "320.00", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "445-Name": "DA Shutter", "555 Shutter-Name": "AnalogIO", "520 Shutter-TriggerSampleRate": "100000", "445 Shutter-IOChannel": "Dev1/ao9", "PRIME-TransposeCorrection": "0", "XYStage-StepSize": "0.1000", "445-Description": "DA device that is used as a shutter", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 AO-IOChannel": "Dev1/ao7", "520 Shutter-Name": "AnalogIO", "COM3-DataBits": "8", "640 AO-Volts": "4.5000", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "555 Shutter-IOChannel": "Dev1/ao13", "WaitInterval": null, "520 Shutter-Sequenceable": "1", "YPositionUm": -180, "445-State": "0", "445-DA Device": "445 Shutter", "PRIME-CircularBufferAutoSize": "ON", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "555 AO-TriggerSampleRate": "100000", "445 Shutter-Volts": "0.0000", "XLIGHT Hub-Port": "COM3", "Core-AutoFocus": "", "COM4-Handshaking": "Off", "528 Shutter-MinVolts": "0.0000", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "445 Shutter-Name": "AnalogIO", "528 Shutter-Sequenceable": "1", "XYStage-TransposeMirrorX": "0", "XYStage-TransposeMirrorY": "0", "PRIME-ReadoutRate": "100MHz 16bit", "XYStage-Name": "Stage", "640 AO-SupportsTriggering": "0", "640-Name": "DA Shutter", "640 Shutter-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "640 AO-Block voltage": "false", "445 Shutter-Sequenceable": "1", "470 AO-MaxVolts": "5.0000", "COM4-BaudRate": "9600", "470-Description": "DA device that is used as a shutter", "520 Shutter-Description": "NI DAC", "PRIME-CCDTemperature": "-14.8500", "Time": "2019-12-18 23:11:02 -0500", "640 AO-TriggerSequenceLength": "1000", "470 AO-Name": "AnalogIO", "XLIGHT Disk slider-Label": "Disk Pos 40um", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "Binning": "1x1", "TE2000-ModelType": "1", "COM3-AnswerTimeout": "500.0000", "555 Shutter-SupportsTriggering": "0", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "TE2000-Port": "COM4", "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Description": "NI DAC", "COM4-AnswerTimeout": "500.0000", "COM1-DelayBetweenCharsMs": "0.0000", "FilterBlock-Label": "Confocal", "445 AO-Name": "AnalogIO", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-Timing-ReadoutTimeNs": "24672000.0000", "528 AO-Demo": "No", "520 AO-Sequenceable": "1", "555-Description": "DA device that is used as a shutter", "405-DA Device": "405 Shutter", "405 AO-TriggerSequenceLength": "1000", "640 AO-MinVolts": "0.0000", "640 Shutter-Block voltage": "false", "FrameIndex": 0, "PRIME-Port": "CMOS", "555 AO-MinVolts": "0.0000", "COM3-Description": "Serial port driver (boost:asio)", "528 AO-Description": "NI DAC", "555 AO-IOChannel": "Dev1/ao6", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "555 AO-TriggerInputLine": "/Dev1/PFI0", "PRIME-ActualInterval-ms": "390.5200", "LudlController-Port": "COM9", "445 Shutter-Demo": "No", "PRIME-Trigger-Expose Out-Mux": "1", "SliceIndex": 0, "PRIME-CCDTemperatureSetPoint": "-15.0000", "405 Shutter-MinVolts": "0.0000", "PRIME-ExposeOutMode": "First Row", "AxisPositions": null, "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Volts": "0.0000", "528-Name": "DA Shutter", "555-State": "0", "XLIGHT Dichroic-Label": "Di03-R635-t1", "445 AO-Sequenceable": "1", "405-Name": "DA Shutter", "405 AO-IOChannel": "Dev1/ao0", "Core-Camera": "PRIME", "Focus-Name": "Focus", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "405 Shutter-IOChannel": "Dev1/ao8", "640 AO-Demo": "No", "640 Shutter-SupportsTriggering": "0", "445 AO-Demo": "No", "555-DA Device": "555 Shutter", "470 Shutter-Description": "NI DAC", "528 AO-Volts": "0.0000", "COM1-DataBits": "8", "Vincent-D3-Address": "x", "520 AO-SupportsTriggering": "0", "405-Description": "DA device that is used as a shutter", "640 Shutter-MinVolts": "0.0000", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "405 AO-Name": "AnalogIO", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "640 AO-TriggerInputLine": "/Dev1/PFI0", "405 AO-TriggerInputLine": "/Dev1/PFI0", "640-Description": "DA device that is used as a shutter", "445 Shutter-TriggerSampleRate": "100000", "470 Shutter-TriggerSequenceLength": "1000", "COM1-StopBits": "1", "520 Shutter-SupportsTriggering": "0", "405-State": "0", "Core-Initialize": "1", "XLIGHT Emission Wheel-State": "3", "520 AO-Description": "NI DAC", "528 AO-TriggerInputLine": "/Dev1/PFI0", "445 Shutter-MaxVolts": "5.0000", "COM9-Handshaking": "Off", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "XLIGHT Spin motor-Label": "On (spinning)", "PRIME-PP 5 ENABLED": "No", "UUID": "69f3229a-19d8-459b-97be-35dd17dac224", "PRIME-TriggerMode": "Internal Trigger", "COM1-Handshaking": "Off", "PRIME-Timing-PostTriggerDelayNs": "49344000.0000", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "Focus-Position": "17741.5000", "528 Shutter-Block voltage": "false", "Channel": "Il-A", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "555 Shutter-Demo": "No", "405 Shutter-Demo": "No", "528 AO-Name": "AnalogIO", "520-State": "0", "Core-Galvo": "", "405 Shutter-TriggerSampleRate": "100000", "Focus-FocusStepSize_(nm)": "100", "PRIME-PixelType": "16bit", "COM4-DataBits": "8", "470 AO-IOChannel": "Dev1/ao2", "520 AO-MinVolts": "0.0000", "COM9-Parity": "None", "PRIME-CircularBufferEnabled": "ON", "PRIME-PP 6 Reset": "No", "640 AO-Description": "NI DAC", "Source": "PRIME", "445 AO-MinVolts": "0.0000", "PIEZO-Name": "Stage", "445 AO-Description": "NI DAC", "PRIME-CircularBufferFrameCount": "46", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "520 AO-Volts": "0.0000", "528 Shutter-TriggerSampleRate": "100000", "XPositionUm": 15712.1, "OpticalPath-State": "4", "PRIME-PP 3 THRESHOLD": "80", "PRIME-PVCAM Version": "3.7.1", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Gain": "1-HDR", "640 Shutter-TriggerSequenceLength": "1000", "528 AO-SupportsTriggering": "0", "445 AO-TriggerInputLine": "/Dev1/PFI0", "OpticalPath-Name": "OpticalPath", "PRIME-PP 4 ENABLED": "Yes", "PRIME-PVCAM Adapter Version": "1.3.29", "COM3-Name": "COM3", "405 AO-MaxVolts": "5.0000", "520 Shutter-Block voltage": "false", "PRIME-Timing-ClearingTimeNs": "49344000.0000", "Core-Focus": "PIEZO", "528 AO-Block voltage": "false", "TE2000-Description": "Nikon TE2000 hub", "528 AO-MinVolts": "0.0000", "COM4-Parity": "None", "Summary": { "Slices": 11, "ChContrastMin": [ 0, 0, 0, 0, 0, 0 ], "PixelAspect": 1, "SlicesFirst": true, "Interval_ms": 0, "Time": "2019-12-18 23:10:50 -0500", "Prefix": "seq_5", "InitialPositionList": [ { "Label": "Pos0", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.21], "Focus": [17741.5], "XYStage": [ 44542.200000000004, 31636.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos1", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.22], "Focus": [17741.4], "XYStage": [ 43801.100000000006, 31637.9 ] }, "GridColumnIndex": 0 }, { "Label": "Pos2", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.5], "XYStage": [ 38561.3, 32040.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos3", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.185], "Focus": [17741.3], "XYStage": [ 37698.4, 32041.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos4", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.16], "Focus": [17741.5], "XYStage": [ 32375.4, 31758.9 ] }, "GridColumnIndex": 0 }, { "Label": "Pos5", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.17000000000002], "Focus": [17741.3], "XYStage": [ 31770.7, 31758.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos6", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.4], "XYStage": [ 26536.800000000003, 31068.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos7", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.205], "Focus": [17741.4], "XYStage": [ 25420.2, 31068.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos8", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.85], "Focus": [17741.3], "XYStage": [ 21059, 31508 ] }, "GridColumnIndex": 0 }, { "Label": "Pos9", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.82], "Focus": [17741.3], "XYStage": [ 19731.9, 31506.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos10", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.79], "Focus": [17741.4], "XYStage": [ 14528.5, 32007.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos11", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.83], "Focus": [17741.2], "XYStage": [ 13830.300000000001, 32009.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos12", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.79], "Focus": [17741.3], "XYStage": [ 8775.300000000001, 32017.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos13", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.815], "Focus": [17741.3], "XYStage": [ 8109.200000000001, 32008.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos14", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.805], "Focus": [17741.3], "XYStage": [ 3483.3, 31908.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos15", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.82], "Focus": [17741.2], "XYStage": [ 2686.8, 31903.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos16", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [211.48000000000002], "Focus": [17741.1], "XYStage": [ -1978.9, 31973.600000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos17", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [211.51500000000001], "Focus": [17741.1], "XYStage": [ -2957.8, 31982.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos18", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.195], "Focus": [17741.5], "XYStage": [ 47747.4, -576.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos19", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.23000000000002], "Focus": [17741.6], "XYStage": [ 47053.9, -576.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos20", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.205], "Focus": [17741.4], "XYStage": [ 41871.200000000004, -479.8 ] }, "GridColumnIndex": 0 }, { "Label": "Pos21", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.4], "XYStage": [ 40882.600000000006, -392 ] }, "GridColumnIndex": 0 }, { "Label": "Pos22", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.24], "Focus": [17741.4], "XYStage": [ 35905.6, -135.3 ] }, "GridColumnIndex": 0 }, { "Label": "Pos23", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.195], "Focus": [17741.5], "XYStage": [ 34990.9, -132.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos24", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.46], "Focus": [17741.4], "XYStage": [ 29678.100000000002, -958.1 ] }, "GridColumnIndex": 0 }, { "Label": "Pos25", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.44], "Focus": [17741.5], "XYStage": [ 28983.600000000002, -939.4000000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos26", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.475], "Focus": [17741.5], "XYStage": [ 23019.7, -122 ] }, "GridColumnIndex": 0 }, { "Label": "Pos27", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.44], "Focus": [17741.7], "XYStage": [ 22428, -116.80000000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos28", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.46], "Focus": [17741.7], "XYStage": [ 16364.900000000001, -181.10000000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos29", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.48000000000002], "Focus": [17741.5], "XYStage": [ 15712.1, -180 ] }, "GridColumnIndex": 0 }, { "Label": "Pos30", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.45000000000002], "Focus": [17741.7], "XYStage": [ 10346.1, 77.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos31", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.49], "Focus": [17741.4], "XYStage": [ 9755.5, 85.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos32", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.475], "Focus": [17741.5], "XYStage": [ 4775.5, 293.90000000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos33", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.445], "Focus": [17741.4], "XYStage": [ 3954.9, 293.90000000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos34", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.455], "Focus": [17741.4], "XYStage": [ -1324.9, 545.6 ] }, "GridColumnIndex": 0 }, { "Label": "Pos35", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.445], "Focus": [17741.3], "XYStage": [ -2097, 576.6 ] }, "GridColumnIndex": 0 } ], "z-step_um": 10, "GridRow": 0, "BitDepth": 16, "Depth": 2, "Channels": 6, "Source": "Micro-Manager", "ChColors": [ -16711681, -256, -65281, -1, -1, -16776961 ], "MetadataVersion": 10, "KeepShutterOpenChannels": true, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536, 65536 ], "CustomIntervals_ms": [], "Height": 1200, "MicroManagerVersion": "1.4.23 20180219", "GridColumn": 0, "UUID": "67598d21-5db5-4842-ab60-f506f7b42c7c", "PixelType": "GRAY16", "Width": 1200, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\Yan\\20191218-YC113-S7S8-miseq", "Frames": 1, "Comment": "", "UserName": "Nikon", "PixelSize_um": 1.1, "ROI": [ 0, 0, 1200, 1200 ], "Date": "2019-12-18", "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC", "P-DAPI-high" ], "KeepShutterOpenSlices": true, "IJType": 1, "ComputerName": "ZadorConfocal2", "Positions": 36, "PositionIndex": 29, "CameraTimeout": 20000, "TimeFirst": false }, "640 AO-Name": "AnalogIO", "Vincent-D3-Command": "Close" }, "FrameKey-0-2-1": { "COM4-StopBits": "1", "528 AO-MaxVolts": "5.0000", "XLIGHT Excitation Wheel-State": "1", "470 Shutter-Name": "AnalogIO", "Core-Shutter": "640", "555 AO-Demo": "No", "XLIGHT Dichroic-State": "2", "XYStage-StepSize-Y": "0.1000", "ZPositionUm": 178.48000000000002, "XYStage-StepSize-X": "0.1000", "PRIME-FirmwareVersion": "20.28", "PRIME-ClearCycles": "2", "520-Name": "DA Shutter", "405 Shutter-Name": "AnalogIO", "PIEZO-StepSize": "0.0050", "PRIME-ClearMode": "Pre-Exposure", "470 AO-MinVolts": "0.0000", "405 AO-SupportsTriggering": "1", "CameraChannelIndex": 0, "XYStage-StartSpeed": "500.0000", "COM9-StopBits": "2", "405 AO-Description": "NI DAC", "COM4-DelayBetweenCharsMs": "0.0000", "445 AO-Block voltage": "false", "520 AO-TriggerInputLine": "/Dev1/PFI0", "555 AO-Description": "NI DAC", "520 AO-TriggerSampleRate": "100000", "COM4-Verbose": "1", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM4-Name": "COM4", "405 Shutter-Volts": "0.0000", "Vincent-D3-Port": "COM1", "PRIME-PP 4 MIN ADU AFFECTED": "200", "470 Shutter-MinVolts": "0.0000", "PRIME-PP 3 ENABLED": "Yes", "470 AO-Description": "NI DAC", "PRIME-Description": "PVCAM API device adapter", "520 Shutter-MaxVolts": "5.0000", "520 Shutter-Volts": "0.0000", "528 AO-Sequenceable": "1", "PRIME-Binning": "1x1", "555 Shutter-MaxVolts": "5.0000", "528 Shutter-TriggerSequenceLength": "1000", "405 Shutter-Block voltage": "false", "XLIGHT Spin motor-State": "1", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "Vincent-D3-Time to open (ms)": "35.0000", "Vincent-D3-Time to close (ms)": "35.0000", "445 AO-TriggerSampleRate": "100000", "640 Shutter-IOChannel": "Dev1/ao14", "555 AO-Sequenceable": "1", "520 AO-Name": "AnalogIO", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "470 AO-Block voltage": "false", "COM1-Verbose": "1", "COM9-AnswerTimeout": "10000.0000", "528 Shutter-Name": "AnalogIO", "PRIME-Trigger Timeout (secs)": "10", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "NextFrame": 0, "470 AO-TriggerSequenceLength": "1000", "PRIME-TransposeXY": "0", "445 AO-TriggerSequenceLength": "1000", "COM1-Name": "COM1", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "COM9-DataBits": "8", "COM1-AnswerTimeout": "500.0000", "Camera": "", "TE2000-Version": "V2.50-V2.50-V2.10", "528 Shutter-Volts": "0.0000", "XLIGHT Hub-Name": "XLIGHT Hub", "PIEZO-Description": "Ludl stage driver adapter", "PositionName": "Pos29", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSampleRate": "100000", "PRIME-FanSpeedSetpoint": "High", "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "405 AO-TriggerSampleRate": "100000", "470 Shutter-Block voltage": "false", "470-State": "0", "COM3-Parity": "None", "405 AO-Volts": "0.0000", "PRIME-TransposeMirrorY": "0", "PRIME-ChipName": "GS144BSI", "470 AO-Volts": "0.0000", "PRIME-TransposeMirrorX": "0", "445 Shutter-SupportsTriggering": "0", "470 Shutter-SupportsTriggering": "0", "XLIGHT Hub-Description": "XLIGHT hub", "PRIME-PreampOffLimit": "10000", "470 AO-Sequenceable": "1", "528 Shutter-MaxVolts": "5.0000", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "Core-XYStage": "XYStage", "470 AO-TriggerSampleRate": "100000", "PRIME-Timing-PreTriggerDelayNs": "24672000.0000", "528-DA Device": "528 Shutter", "Focus-Description": "Nikon TE2000 focus adapter", "FilterBlock-State": "5", "520 AO-Demo": "No", "COM3-DelayBetweenCharsMs": "0.0000", "470 Shutter-Volts": "0.0000", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "PRIME-PP 2 THRESHOLD": "125", "PRIME-PP 4 THRESHOLD": "75", "XYStage-Acceleration": "4.0000", "470 Shutter-Demo": "No", "PRIME-Name": "PMPCIECam00", "640-DA Device": "640 Shutter", "405 AO-Block voltage": "false", "ElapsedTime-ms": 1203342, "PRIME-Offset": "100", "445 AO-IOChannel": "Dev1/ao1", "640 AO-MaxVolts": "5.0000", "PositionIndex": 29, "PRIME-PP 1 ENABLED": "Yes", "COM1-BaudRate": "9600", "640-State": "1", "COM3-Verbose": "1", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "LudlController-TransmissionDelay": "0", "PRIME-FullWellCapacity": "62000", "PRIME-SMARTStreamingEnabled": "No", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "640 Shutter-Description": "NI DAC", "528-Description": "DA device that is used as a shutter", "COM4-Description": "Serial port driver (boost:asio)", "405 Shutter-Description": "NI DAC", "TE2000-Name": "TE2000", "528 Shutter-Description": "NI DAC", "COM9-Description": "Serial port driver (boost:asio)", "LudlController-Name": "LudlController", "445 Shutter-TriggerSequenceLength": "1000", "PRIME-SerialNumber": "A18B203008", "445 AO-Volts": "0.0000", "PRIME-X-dimension": "1200", "PRIME-ShutterCloseDelay": "0", "XLIGHT Disk slider-State": "2", "Position": "Default", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 AO-IOChannel": "Dev1/ao5", "520 AO-IOChannel": "Dev1/ao4", "528 AO-TriggerSequenceLength": "1000", "405 Shutter-MaxVolts": "5.0000", "470-DA Device": "470 Shutter", "PRIME-FTCapable": "No", "Core-ChannelGroup": "Channels", "555 AO-SupportsTriggering": "0", "PRIME-Y-dimension": "1200", "Frame": 0, "555 Shutter-Sequenceable": "1", "PRIME-Timing-ExposureTimeNs": "499998000.0000", "Core-ImageProcessor": "", "528-State": "0", "520 AO-Block voltage": "false", "XLIGHT Excitation Wheel-Label": "QUAD", "640 Shutter-Demo": "No", "COM3-StopBits": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "520 Shutter-IOChannel": "Dev1/ao11", "Width": 1200, "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-SupportsTriggering": "0", "640 AO-TriggerSampleRate": "100000", "520-Description": "DA device that is used as a shutter", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "405 Shutter-Sequenceable": "1", "405 Shutter-SupportsTriggering": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-TriggerSampleRate": "100000", "640 Shutter-MaxVolts": "5.0000", "555 AO-MaxVolts": "5.0000", "520 AO-TriggerSequenceLength": "1000", "COM3-BaudRate": "9600", "COM9-BaudRate": "9600", "Vincent-D3-Channel #": "Ch. #1", "PRIME-PMode": "Normal", "OpticalPath-Label": "Left(100)", "BitDepth": 16, "555 AO-TriggerSequenceLength": "1000", "445 Shutter-Description": "NI DAC", "405 AO-Sequenceable": "1", "PRIME-AcquisitionMethod": "Callbacks", "555 AO-Name": "AnalogIO", "PRIME-ShutterMode": "Pre-Exposure", "COM3-Handshaking": "Off", "445 AO-SupportsTriggering": "0", "ROI": "0-0-1200-1200", "COM9-Verbose": "1", "PRIME-Color": "OFF", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "PRIME-ShutterOpenDelay": "0", "COM1-Parity": "None", "Slice": 1, "SlicePosition": -40, "COM9-DelayBetweenCharsMs": "0.0000", "555-Name": "DA Shutter", "PIEZO-LudlSingleAxisName": "B", "XLIGHT Emission Wheel-Label": "679/29 (A)", "640 Shutter-Name": "AnalogIO", "640 AO-Sequenceable": "1", "520 Shutter-Demo": "No", "528 Shutter-Demo": "No", "470-Name": "DA Shutter", "520-DA Device": "520 Shutter", "XYStage-Description": "Ludl XY stage driver adapter", "445 Shutter-MinVolts": "0.0000", "528 AO-TriggerSampleRate": "100000", "405 AO-MinVolts": "0.0000", "405 Shutter-TriggerSequenceLength": "1000", "Height": 1200, "555 AO-Block voltage": "false", "640 Shutter-Volts": "5.0000", "PixelType": "GRAY16", "445 Shutter-Block voltage": "false", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "520 Shutter-TriggerSequenceLength": "1000", "COM9-Name": "COM9", "Core-SLM": "", "555 AO-Volts": "0.0000", "555 Shutter-Block voltage": "false", "XYStage-Speed": "1800.0000", "520 AO-MaxVolts": "5.0000", "528 Shutter-SupportsTriggering": "0", "COM1-Description": "Serial port driver (boost:asio)", "PRIME-MetadataEnabled": "No", "520 Shutter-MinVolts": "0.0000", "470 Shutter-Sequenceable": "1", "PRIME-PreampDelay": "0", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-MinVolts": "0.0000", "ChannelIndex": 2, "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Core-AutoShutter": "1", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "Exposure-ms": 320, "PRIME-PP 2 ENABLED": "Yes", "640 Shutter-TriggerSampleRate": "100000", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "470 Shutter-IOChannel": "Dev1/ao10", "405 AO-Demo": "No", "PixelSizeUm": 1.1, "470 AO-Demo": "No", "470 Shutter-MaxVolts": "5.0000", "Vincent-D3-Name": "Vincent-D3", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FileName": "img_000000000_Il-A_001.tif", "LudlController-Reset": "Operate", "PRIME-Exposure": "320.00", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "445-Name": "DA Shutter", "555 Shutter-Name": "AnalogIO", "520 Shutter-TriggerSampleRate": "100000", "445 Shutter-IOChannel": "Dev1/ao9", "PRIME-TransposeCorrection": "0", "XYStage-StepSize": "0.1000", "445-Description": "DA device that is used as a shutter", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 AO-IOChannel": "Dev1/ao7", "520 Shutter-Name": "AnalogIO", "COM3-DataBits": "8", "640 AO-Volts": "4.5000", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "555 Shutter-IOChannel": "Dev1/ao13", "WaitInterval": null, "520 Shutter-Sequenceable": "1", "YPositionUm": -180, "445-State": "0", "445-DA Device": "445 Shutter", "PRIME-CircularBufferAutoSize": "ON", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "555 AO-TriggerSampleRate": "100000", "445 Shutter-Volts": "0.0000", "XLIGHT Hub-Port": "COM3", "Core-AutoFocus": "", "COM4-Handshaking": "Off", "528 Shutter-MinVolts": "0.0000", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "445 Shutter-Name": "AnalogIO", "528 Shutter-Sequenceable": "1", "XYStage-TransposeMirrorX": "0", "XYStage-TransposeMirrorY": "0", "PRIME-ReadoutRate": "100MHz 16bit", "XYStage-Name": "Stage", "640 AO-SupportsTriggering": "0", "640-Name": "DA Shutter", "640 Shutter-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "640 AO-Block voltage": "false", "445 Shutter-Sequenceable": "1", "470 AO-MaxVolts": "5.0000", "COM4-BaudRate": "9600", "470-Description": "DA device that is used as a shutter", "520 Shutter-Description": "NI DAC", "PRIME-CCDTemperature": "-14.8500", "Time": "2019-12-18 23:11:03 -0500", "640 AO-TriggerSequenceLength": "1000", "470 AO-Name": "AnalogIO", "XLIGHT Disk slider-Label": "Disk Pos 40um", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "Binning": "1x1", "TE2000-ModelType": "1", "COM3-AnswerTimeout": "500.0000", "555 Shutter-SupportsTriggering": "0", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "TE2000-Port": "COM4", "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Description": "NI DAC", "COM4-AnswerTimeout": "500.0000", "COM1-DelayBetweenCharsMs": "0.0000", "FilterBlock-Label": "Confocal", "445 AO-Name": "AnalogIO", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-Timing-ReadoutTimeNs": "24672000.0000", "528 AO-Demo": "No", "520 AO-Sequenceable": "1", "555-Description": "DA device that is used as a shutter", "405-DA Device": "405 Shutter", "405 AO-TriggerSequenceLength": "1000", "640 AO-MinVolts": "0.0000", "640 Shutter-Block voltage": "false", "FrameIndex": 0, "PRIME-Port": "CMOS", "555 AO-MinVolts": "0.0000", "COM3-Description": "Serial port driver (boost:asio)", "528 AO-Description": "NI DAC", "555 AO-IOChannel": "Dev1/ao6", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "555 AO-TriggerInputLine": "/Dev1/PFI0", "PRIME-ActualInterval-ms": "390.5200", "LudlController-Port": "COM9", "445 Shutter-Demo": "No", "PRIME-Trigger-Expose Out-Mux": "1", "SliceIndex": 1, "PRIME-CCDTemperatureSetPoint": "-15.0000", "405 Shutter-MinVolts": "0.0000", "PRIME-ExposeOutMode": "First Row", "AxisPositions": null, "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Volts": "0.0000", "528-Name": "DA Shutter", "555-State": "0", "XLIGHT Dichroic-Label": "Di03-R635-t1", "445 AO-Sequenceable": "1", "405-Name": "DA Shutter", "405 AO-IOChannel": "Dev1/ao0", "Core-Camera": "PRIME", "Focus-Name": "Focus", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "405 Shutter-IOChannel": "Dev1/ao8", "640 AO-Demo": "No", "640 Shutter-SupportsTriggering": "0", "445 AO-Demo": "No", "555-DA Device": "555 Shutter", "470 Shutter-Description": "NI DAC", "528 AO-Volts": "0.0000", "COM1-DataBits": "8", "Vincent-D3-Address": "x", "520 AO-SupportsTriggering": "0", "405-Description": "DA device that is used as a shutter", "640 Shutter-MinVolts": "0.0000", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "405 AO-Name": "AnalogIO", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "640 AO-TriggerInputLine": "/Dev1/PFI0", "405 AO-TriggerInputLine": "/Dev1/PFI0", "640-Description": "DA device that is used as a shutter", "445 Shutter-TriggerSampleRate": "100000", "470 Shutter-TriggerSequenceLength": "1000", "COM1-StopBits": "1", "520 Shutter-SupportsTriggering": "0", "405-State": "0", "Core-Initialize": "1", "XLIGHT Emission Wheel-State": "3", "520 AO-Description": "NI DAC", "528 AO-TriggerInputLine": "/Dev1/PFI0", "445 Shutter-MaxVolts": "5.0000", "COM9-Handshaking": "Off", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "XLIGHT Spin motor-Label": "On (spinning)", "PRIME-PP 5 ENABLED": "No", "UUID": "ef67c2f4-54aa-4d1a-afd8-23a6dd9db3ea", "PRIME-TriggerMode": "Internal Trigger", "COM1-Handshaking": "Off", "PRIME-Timing-PostTriggerDelayNs": "49344000.0000", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "Focus-Position": "17741.5000", "528 Shutter-Block voltage": "false", "Channel": "Il-A", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "555 Shutter-Demo": "No", "405 Shutter-Demo": "No", "528 AO-Name": "AnalogIO", "520-State": "0", "Core-Galvo": "", "405 Shutter-TriggerSampleRate": "100000", "Focus-FocusStepSize_(nm)": "100", "PRIME-PixelType": "16bit", "COM4-DataBits": "8", "470 AO-IOChannel": "Dev1/ao2", "520 AO-MinVolts": "0.0000", "COM9-Parity": "None", "PRIME-CircularBufferEnabled": "ON", "PRIME-PP 6 Reset": "No", "640 AO-Description": "NI DAC", "Source": "PRIME", "445 AO-MinVolts": "0.0000", "PIEZO-Name": "Stage", "445 AO-Description": "NI DAC", "PRIME-CircularBufferFrameCount": "46", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "520 AO-Volts": "0.0000", "528 Shutter-TriggerSampleRate": "100000", "XPositionUm": 15712.1, "OpticalPath-State": "4", "PRIME-PP 3 THRESHOLD": "80", "PRIME-PVCAM Version": "3.7.1", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Gain": "1-HDR", "640 Shutter-TriggerSequenceLength": "1000", "528 AO-SupportsTriggering": "0", "445 AO-TriggerInputLine": "/Dev1/PFI0", "OpticalPath-Name": "OpticalPath", "PRIME-PP 4 ENABLED": "Yes", "PRIME-PVCAM Adapter Version": "1.3.29", "COM3-Name": "COM3", "405 AO-MaxVolts": "5.0000", "520 Shutter-Block voltage": "false", "PRIME-Timing-ClearingTimeNs": "49344000.0000", "Core-Focus": "PIEZO", "528 AO-Block voltage": "false", "TE2000-Description": "Nikon TE2000 hub", "528 AO-MinVolts": "0.0000", "COM4-Parity": "None", "Summary": { "Slices": 11, "ChContrastMin": [ 0, 0, 0, 0, 0, 0 ], "PixelAspect": 1, "SlicesFirst": true, "Interval_ms": 0, "Time": "2019-12-18 23:10:50 -0500", "Prefix": "seq_5", "InitialPositionList": [ { "Label": "Pos0", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.21], "Focus": [17741.5], "XYStage": [ 44542.200000000004, 31636.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos1", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.22], "Focus": [17741.4], "XYStage": [ 43801.100000000006, 31637.9 ] }, "GridColumnIndex": 0 }, { "Label": "Pos2", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.5], "XYStage": [ 38561.3, 32040.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos3", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.185], "Focus": [17741.3], "XYStage": [ 37698.4, 32041.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos4", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.16], "Focus": [17741.5], "XYStage": [ 32375.4, 31758.9 ] }, "GridColumnIndex": 0 }, { "Label": "Pos5", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.17000000000002], "Focus": [17741.3], "XYStage": [ 31770.7, 31758.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos6", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.4], "XYStage": [ 26536.800000000003, 31068.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos7", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.205], "Focus": [17741.4], "XYStage": [ 25420.2, 31068.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos8", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.85], "Focus": [17741.3], "XYStage": [ 21059, 31508 ] }, "GridColumnIndex": 0 }, { "Label": "Pos9", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.82], "Focus": [17741.3], "XYStage": [ 19731.9, 31506.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos10", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.79], "Focus": [17741.4], "XYStage": [ 14528.5, 32007.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos11", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.83], "Focus": [17741.2], "XYStage": [ 13830.300000000001, 32009.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos12", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.79], "Focus": [17741.3], "XYStage": [ 8775.300000000001, 32017.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos13", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.815], "Focus": [17741.3], "XYStage": [ 8109.200000000001, 32008.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos14", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.805], "Focus": [17741.3], "XYStage": [ 3483.3, 31908.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos15", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.82], "Focus": [17741.2], "XYStage": [ 2686.8, 31903.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos16", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [211.48000000000002], "Focus": [17741.1], "XYStage": [ -1978.9, 31973.600000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos17", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [211.51500000000001], "Focus": [17741.1], "XYStage": [ -2957.8, 31982.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos18", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.195], "Focus": [17741.5], "XYStage": [ 47747.4, -576.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos19", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.23000000000002], "Focus": [17741.6], "XYStage": [ 47053.9, -576.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos20", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.205], "Focus": [17741.4], "XYStage": [ 41871.200000000004, -479.8 ] }, "GridColumnIndex": 0 }, { "Label": "Pos21", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.4], "XYStage": [ 40882.600000000006, -392 ] }, "GridColumnIndex": 0 }, { "Label": "Pos22", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.24], "Focus": [17741.4], "XYStage": [ 35905.6, -135.3 ] }, "GridColumnIndex": 0 }, { "Label": "Pos23", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.195], "Focus": [17741.5], "XYStage": [ 34990.9, -132.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos24", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.46], "Focus": [17741.4], "XYStage": [ 29678.100000000002, -958.1 ] }, "GridColumnIndex": 0 }, { "Label": "Pos25", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.44], "Focus": [17741.5], "XYStage": [ 28983.600000000002, -939.4000000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos26", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.475], "Focus": [17741.5], "XYStage": [ 23019.7, -122 ] }, "GridColumnIndex": 0 }, { "Label": "Pos27", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.44], "Focus": [17741.7], "XYStage": [ 22428, -116.80000000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos28", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.46], "Focus": [17741.7], "XYStage": [ 16364.900000000001, -181.10000000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos29", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.48000000000002], "Focus": [17741.5], "XYStage": [ 15712.1, -180 ] }, "GridColumnIndex": 0 }, { "Label": "Pos30", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.45000000000002], "Focus": [17741.7], "XYStage": [ 10346.1, 77.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos31", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.49], "Focus": [17741.4], "XYStage": [ 9755.5, 85.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos32", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.475], "Focus": [17741.5], "XYStage": [ 4775.5, 293.90000000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos33", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.445], "Focus": [17741.4], "XYStage": [ 3954.9, 293.90000000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos34", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.455], "Focus": [17741.4], "XYStage": [ -1324.9, 545.6 ] }, "GridColumnIndex": 0 }, { "Label": "Pos35", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.445], "Focus": [17741.3], "XYStage": [ -2097, 576.6 ] }, "GridColumnIndex": 0 } ], "z-step_um": 10, "GridRow": 0, "BitDepth": 16, "Depth": 2, "Channels": 6, "Source": "Micro-Manager", "ChColors": [ -16711681, -256, -65281, -1, -1, -16776961 ], "MetadataVersion": 10, "KeepShutterOpenChannels": true, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536, 65536 ], "CustomIntervals_ms": [], "Height": 1200, "MicroManagerVersion": "1.4.23 20180219", "GridColumn": 0, "UUID": "67598d21-5db5-4842-ab60-f506f7b42c7c", "PixelType": "GRAY16", "Width": 1200, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\Yan\\20191218-YC113-S7S8-miseq", "Frames": 1, "Comment": "", "UserName": "Nikon", "PixelSize_um": 1.1, "ROI": [ 0, 0, 1200, 1200 ], "Date": "2019-12-18", "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC", "P-DAPI-high" ], "KeepShutterOpenSlices": true, "IJType": 1, "ComputerName": "ZadorConfocal2", "Positions": 36, "PositionIndex": 29, "CameraTimeout": 20000, "TimeFirst": false }, "640 AO-Name": "AnalogIO", "Vincent-D3-Command": "Close" }, "FrameKey-0-2-2": { "COM4-StopBits": "1", "528 AO-MaxVolts": "5.0000", "XLIGHT Excitation Wheel-State": "1", "470 Shutter-Name": "AnalogIO", "Core-Shutter": "640", "555 AO-Demo": "No", "XLIGHT Dichroic-State": "2", "XYStage-StepSize-Y": "0.1000", "ZPositionUm": 188.48000000000002, "XYStage-StepSize-X": "0.1000", "PRIME-FirmwareVersion": "20.28", "PRIME-ClearCycles": "2", "520-Name": "DA Shutter", "405 Shutter-Name": "AnalogIO", "PIEZO-StepSize": "0.0050", "PRIME-ClearMode": "Pre-Exposure", "470 AO-MinVolts": "0.0000", "405 AO-SupportsTriggering": "1", "CameraChannelIndex": 0, "XYStage-StartSpeed": "500.0000", "COM9-StopBits": "2", "405 AO-Description": "NI DAC", "COM4-DelayBetweenCharsMs": "0.0000", "445 AO-Block voltage": "false", "520 AO-TriggerInputLine": "/Dev1/PFI0", "555 AO-Description": "NI DAC", "520 AO-TriggerSampleRate": "100000", "COM4-Verbose": "1", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM4-Name": "COM4", "405 Shutter-Volts": "0.0000", "Vincent-D3-Port": "COM1", "PRIME-PP 4 MIN ADU AFFECTED": "200", "470 Shutter-MinVolts": "0.0000", "PRIME-PP 3 ENABLED": "Yes", "470 AO-Description": "NI DAC", "PRIME-Description": "PVCAM API device adapter", "520 Shutter-MaxVolts": "5.0000", "520 Shutter-Volts": "0.0000", "528 AO-Sequenceable": "1", "PRIME-Binning": "1x1", "555 Shutter-MaxVolts": "5.0000", "528 Shutter-TriggerSequenceLength": "1000", "405 Shutter-Block voltage": "false", "XLIGHT Spin motor-State": "1", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "Vincent-D3-Time to open (ms)": "35.0000", "Vincent-D3-Time to close (ms)": "35.0000", "445 AO-TriggerSampleRate": "100000", "640 Shutter-IOChannel": "Dev1/ao14", "555 AO-Sequenceable": "1", "520 AO-Name": "AnalogIO", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "470 AO-Block voltage": "false", "COM1-Verbose": "1", "COM9-AnswerTimeout": "10000.0000", "528 Shutter-Name": "AnalogIO", "PRIME-Trigger Timeout (secs)": "10", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "NextFrame": 0, "470 AO-TriggerSequenceLength": "1000", "PRIME-TransposeXY": "0", "445 AO-TriggerSequenceLength": "1000", "COM1-Name": "COM1", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "COM9-DataBits": "8", "COM1-AnswerTimeout": "500.0000", "Camera": "", "TE2000-Version": "V2.50-V2.50-V2.10", "528 Shutter-Volts": "0.0000", "XLIGHT Hub-Name": "XLIGHT Hub", "PIEZO-Description": "Ludl stage driver adapter", "PositionName": "Pos29", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSampleRate": "100000", "PRIME-FanSpeedSetpoint": "High", "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "405 AO-TriggerSampleRate": "100000", "470 Shutter-Block voltage": "false", "470-State": "0", "COM3-Parity": "None", "405 AO-Volts": "0.0000", "PRIME-TransposeMirrorY": "0", "PRIME-ChipName": "GS144BSI", "470 AO-Volts": "0.0000", "PRIME-TransposeMirrorX": "0", "445 Shutter-SupportsTriggering": "0", "470 Shutter-SupportsTriggering": "0", "XLIGHT Hub-Description": "XLIGHT hub", "PRIME-PreampOffLimit": "10000", "470 AO-Sequenceable": "1", "528 Shutter-MaxVolts": "5.0000", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "Core-XYStage": "XYStage", "470 AO-TriggerSampleRate": "100000", "PRIME-Timing-PreTriggerDelayNs": "24672000.0000", "528-DA Device": "528 Shutter", "Focus-Description": "Nikon TE2000 focus adapter", "FilterBlock-State": "5", "520 AO-Demo": "No", "COM3-DelayBetweenCharsMs": "0.0000", "470 Shutter-Volts": "0.0000", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "PRIME-PP 2 THRESHOLD": "125", "PRIME-PP 4 THRESHOLD": "75", "XYStage-Acceleration": "4.0000", "470 Shutter-Demo": "No", "PRIME-Name": "PMPCIECam00", "640-DA Device": "640 Shutter", "405 AO-Block voltage": "false", "ElapsedTime-ms": 1203954, "PRIME-Offset": "100", "445 AO-IOChannel": "Dev1/ao1", "640 AO-MaxVolts": "5.0000", "PositionIndex": 29, "PRIME-PP 1 ENABLED": "Yes", "COM1-BaudRate": "9600", "640-State": "1", "COM3-Verbose": "1", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "LudlController-TransmissionDelay": "0", "PRIME-FullWellCapacity": "62000", "PRIME-SMARTStreamingEnabled": "No", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "640 Shutter-Description": "NI DAC", "528-Description": "DA device that is used as a shutter", "COM4-Description": "Serial port driver (boost:asio)", "405 Shutter-Description": "NI DAC", "TE2000-Name": "TE2000", "528 Shutter-Description": "NI DAC", "COM9-Description": "Serial port driver (boost:asio)", "LudlController-Name": "LudlController", "445 Shutter-TriggerSequenceLength": "1000", "PRIME-SerialNumber": "A18B203008", "445 AO-Volts": "0.0000", "PRIME-X-dimension": "1200", "PRIME-ShutterCloseDelay": "0", "XLIGHT Disk slider-State": "2", "Position": "Default", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 AO-IOChannel": "Dev1/ao5", "520 AO-IOChannel": "Dev1/ao4", "528 AO-TriggerSequenceLength": "1000", "405 Shutter-MaxVolts": "5.0000", "470-DA Device": "470 Shutter", "PRIME-FTCapable": "No", "Core-ChannelGroup": "Channels", "555 AO-SupportsTriggering": "0", "PRIME-Y-dimension": "1200", "Frame": 0, "555 Shutter-Sequenceable": "1", "PRIME-Timing-ExposureTimeNs": "499998000.0000", "Core-ImageProcessor": "", "528-State": "0", "520 AO-Block voltage": "false", "XLIGHT Excitation Wheel-Label": "QUAD", "640 Shutter-Demo": "No", "COM3-StopBits": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "520 Shutter-IOChannel": "Dev1/ao11", "Width": 1200, "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-SupportsTriggering": "0", "640 AO-TriggerSampleRate": "100000", "520-Description": "DA device that is used as a shutter", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "405 Shutter-Sequenceable": "1", "405 Shutter-SupportsTriggering": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-TriggerSampleRate": "100000", "640 Shutter-MaxVolts": "5.0000", "555 AO-MaxVolts": "5.0000", "520 AO-TriggerSequenceLength": "1000", "COM3-BaudRate": "9600", "COM9-BaudRate": "9600", "Vincent-D3-Channel #": "Ch. #1", "PRIME-PMode": "Normal", "OpticalPath-Label": "Left(100)", "BitDepth": 16, "555 AO-TriggerSequenceLength": "1000", "445 Shutter-Description": "NI DAC", "405 AO-Sequenceable": "1", "PRIME-AcquisitionMethod": "Callbacks", "555 AO-Name": "AnalogIO", "PRIME-ShutterMode": "Pre-Exposure", "COM3-Handshaking": "Off", "445 AO-SupportsTriggering": "0", "ROI": "0-0-1200-1200", "COM9-Verbose": "1", "PRIME-Color": "OFF", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "PRIME-ShutterOpenDelay": "0", "COM1-Parity": "None", "Slice": 2, "SlicePosition": -30, "COM9-DelayBetweenCharsMs": "0.0000", "555-Name": "DA Shutter", "PIEZO-LudlSingleAxisName": "B", "XLIGHT Emission Wheel-Label": "679/29 (A)", "640 Shutter-Name": "AnalogIO", "640 AO-Sequenceable": "1", "520 Shutter-Demo": "No", "528 Shutter-Demo": "No", "470-Name": "DA Shutter", "520-DA Device": "520 Shutter", "XYStage-Description": "Ludl XY stage driver adapter", "445 Shutter-MinVolts": "0.0000", "528 AO-TriggerSampleRate": "100000", "405 AO-MinVolts": "0.0000", "405 Shutter-TriggerSequenceLength": "1000", "Height": 1200, "555 AO-Block voltage": "false", "640 Shutter-Volts": "5.0000", "PixelType": "GRAY16", "445 Shutter-Block voltage": "false", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "520 Shutter-TriggerSequenceLength": "1000", "COM9-Name": "COM9", "Core-SLM": "", "555 AO-Volts": "0.0000", "555 Shutter-Block voltage": "false", "XYStage-Speed": "1800.0000", "520 AO-MaxVolts": "5.0000", "528 Shutter-SupportsTriggering": "0", "COM1-Description": "Serial port driver (boost:asio)", "PRIME-MetadataEnabled": "No", "520 Shutter-MinVolts": "0.0000", "470 Shutter-Sequenceable": "1", "PRIME-PreampDelay": "0", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-MinVolts": "0.0000", "ChannelIndex": 2, "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Core-AutoShutter": "1", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "Exposure-ms": 320, "PRIME-PP 2 ENABLED": "Yes", "640 Shutter-TriggerSampleRate": "100000", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "470 Shutter-IOChannel": "Dev1/ao10", "405 AO-Demo": "No", "PixelSizeUm": 1.1, "470 AO-Demo": "No", "470 Shutter-MaxVolts": "5.0000", "Vincent-D3-Name": "Vincent-D3", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FileName": "img_000000000_Il-A_002.tif", "LudlController-Reset": "Operate", "PRIME-Exposure": "320.00", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "445-Name": "DA Shutter", "555 Shutter-Name": "AnalogIO", "520 Shutter-TriggerSampleRate": "100000", "445 Shutter-IOChannel": "Dev1/ao9", "PRIME-TransposeCorrection": "0", "XYStage-StepSize": "0.1000", "445-Description": "DA device that is used as a shutter", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 AO-IOChannel": "Dev1/ao7", "520 Shutter-Name": "AnalogIO", "COM3-DataBits": "8", "640 AO-Volts": "4.5000", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "555 Shutter-IOChannel": "Dev1/ao13", "WaitInterval": null, "520 Shutter-Sequenceable": "1", "YPositionUm": -180, "445-State": "0", "445-DA Device": "445 Shutter", "PRIME-CircularBufferAutoSize": "ON", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "555 AO-TriggerSampleRate": "100000", "445 Shutter-Volts": "0.0000", "XLIGHT Hub-Port": "COM3", "Core-AutoFocus": "", "COM4-Handshaking": "Off", "528 Shutter-MinVolts": "0.0000", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "445 Shutter-Name": "AnalogIO", "528 Shutter-Sequenceable": "1", "XYStage-TransposeMirrorX": "0", "XYStage-TransposeMirrorY": "0", "PRIME-ReadoutRate": "100MHz 16bit", "XYStage-Name": "Stage", "640 AO-SupportsTriggering": "0", "640-Name": "DA Shutter", "640 Shutter-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "640 AO-Block voltage": "false", "445 Shutter-Sequenceable": "1", "470 AO-MaxVolts": "5.0000", "COM4-BaudRate": "9600", "470-Description": "DA device that is used as a shutter", "520 Shutter-Description": "NI DAC", "PRIME-CCDTemperature": "-14.8500", "Time": "2019-12-18 23:11:04 -0500", "640 AO-TriggerSequenceLength": "1000", "470 AO-Name": "AnalogIO", "XLIGHT Disk slider-Label": "Disk Pos 40um", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "Binning": "1x1", "TE2000-ModelType": "1", "COM3-AnswerTimeout": "500.0000", "555 Shutter-SupportsTriggering": "0", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "TE2000-Port": "COM4", "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Description": "NI DAC", "COM4-AnswerTimeout": "500.0000", "COM1-DelayBetweenCharsMs": "0.0000", "FilterBlock-Label": "Confocal", "445 AO-Name": "AnalogIO", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-Timing-ReadoutTimeNs": "24672000.0000", "528 AO-Demo": "No", "520 AO-Sequenceable": "1", "555-Description": "DA device that is used as a shutter", "405-DA Device": "405 Shutter", "405 AO-TriggerSequenceLength": "1000", "640 AO-MinVolts": "0.0000", "640 Shutter-Block voltage": "false", "FrameIndex": 0, "PRIME-Port": "CMOS", "555 AO-MinVolts": "0.0000", "COM3-Description": "Serial port driver (boost:asio)", "528 AO-Description": "NI DAC", "555 AO-IOChannel": "Dev1/ao6", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "555 AO-TriggerInputLine": "/Dev1/PFI0", "PRIME-ActualInterval-ms": "390.5200", "LudlController-Port": "COM9", "445 Shutter-Demo": "No", "PRIME-Trigger-Expose Out-Mux": "1", "SliceIndex": 2, "PRIME-CCDTemperatureSetPoint": "-15.0000", "405 Shutter-MinVolts": "0.0000", "PRIME-ExposeOutMode": "First Row", "AxisPositions": null, "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Volts": "0.0000", "528-Name": "DA Shutter", "555-State": "0", "XLIGHT Dichroic-Label": "Di03-R635-t1", "445 AO-Sequenceable": "1", "405-Name": "DA Shutter", "405 AO-IOChannel": "Dev1/ao0", "Core-Camera": "PRIME", "Focus-Name": "Focus", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "405 Shutter-IOChannel": "Dev1/ao8", "640 AO-Demo": "No", "640 Shutter-SupportsTriggering": "0", "445 AO-Demo": "No", "555-DA Device": "555 Shutter", "470 Shutter-Description": "NI DAC", "528 AO-Volts": "0.0000", "COM1-DataBits": "8", "Vincent-D3-Address": "x", "520 AO-SupportsTriggering": "0", "405-Description": "DA device that is used as a shutter", "640 Shutter-MinVolts": "0.0000", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "405 AO-Name": "AnalogIO", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "640 AO-TriggerInputLine": "/Dev1/PFI0", "405 AO-TriggerInputLine": "/Dev1/PFI0", "640-Description": "DA device that is used as a shutter", "445 Shutter-TriggerSampleRate": "100000", "470 Shutter-TriggerSequenceLength": "1000", "COM1-StopBits": "1", "520 Shutter-SupportsTriggering": "0", "405-State": "0", "Core-Initialize": "1", "XLIGHT Emission Wheel-State": "3", "520 AO-Description": "NI DAC", "528 AO-TriggerInputLine": "/Dev1/PFI0", "445 Shutter-MaxVolts": "5.0000", "COM9-Handshaking": "Off", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "XLIGHT Spin motor-Label": "On (spinning)", "PRIME-PP 5 ENABLED": "No", "UUID": "ace35620-6de5-4119-8f2e-29d7e342c7ac", "PRIME-TriggerMode": "Internal Trigger", "COM1-Handshaking": "Off", "PRIME-Timing-PostTriggerDelayNs": "49344000.0000", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "Focus-Position": "17741.5000", "528 Shutter-Block voltage": "false", "Channel": "Il-A", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "555 Shutter-Demo": "No", "405 Shutter-Demo": "No", "528 AO-Name": "AnalogIO", "520-State": "0", "Core-Galvo": "", "405 Shutter-TriggerSampleRate": "100000", "Focus-FocusStepSize_(nm)": "100", "PRIME-PixelType": "16bit", "COM4-DataBits": "8", "470 AO-IOChannel": "Dev1/ao2", "520 AO-MinVolts": "0.0000", "COM9-Parity": "None", "PRIME-CircularBufferEnabled": "ON", "PRIME-PP 6 Reset": "No", "640 AO-Description": "NI DAC", "Source": "PRIME", "445 AO-MinVolts": "0.0000", "PIEZO-Name": "Stage", "445 AO-Description": "NI DAC", "PRIME-CircularBufferFrameCount": "46", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "520 AO-Volts": "0.0000", "528 Shutter-TriggerSampleRate": "100000", "XPositionUm": 15712.1, "OpticalPath-State": "4", "PRIME-PP 3 THRESHOLD": "80", "PRIME-PVCAM Version": "3.7.1", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Gain": "1-HDR", "640 Shutter-TriggerSequenceLength": "1000", "528 AO-SupportsTriggering": "0", "445 AO-TriggerInputLine": "/Dev1/PFI0", "OpticalPath-Name": "OpticalPath", "PRIME-PP 4 ENABLED": "Yes", "PRIME-PVCAM Adapter Version": "1.3.29", "COM3-Name": "COM3", "405 AO-MaxVolts": "5.0000", "520 Shutter-Block voltage": "false", "PRIME-Timing-ClearingTimeNs": "49344000.0000", "Core-Focus": "PIEZO", "528 AO-Block voltage": "false", "TE2000-Description": "Nikon TE2000 hub", "528 AO-MinVolts": "0.0000", "COM4-Parity": "None", "Summary": { "Slices": 11, "ChContrastMin": [ 0, 0, 0, 0, 0, 0 ], "PixelAspect": 1, "SlicesFirst": true, "Interval_ms": 0, "Time": "2019-12-18 23:10:50 -0500", "Prefix": "seq_5", "InitialPositionList": [ { "Label": "Pos0", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.21], "Focus": [17741.5], "XYStage": [ 44542.200000000004, 31636.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos1", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.22], "Focus": [17741.4], "XYStage": [ 43801.100000000006, 31637.9 ] }, "GridColumnIndex": 0 }, { "Label": "Pos2", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.5], "XYStage": [ 38561.3, 32040.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos3", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.185], "Focus": [17741.3], "XYStage": [ 37698.4, 32041.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos4", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.16], "Focus": [17741.5], "XYStage": [ 32375.4, 31758.9 ] }, "GridColumnIndex": 0 }, { "Label": "Pos5", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.17000000000002], "Focus": [17741.3], "XYStage": [ 31770.7, 31758.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos6", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.4], "XYStage": [ 26536.800000000003, 31068.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos7", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.205], "Focus": [17741.4], "XYStage": [ 25420.2, 31068.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos8", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.85], "Focus": [17741.3], "XYStage": [ 21059, 31508 ] }, "GridColumnIndex": 0 }, { "Label": "Pos9", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.82], "Focus": [17741.3], "XYStage": [ 19731.9, 31506.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos10", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.79], "Focus": [17741.4], "XYStage": [ 14528.5, 32007.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos11", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.83], "Focus": [17741.2], "XYStage": [ 13830.300000000001, 32009.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos12", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.79], "Focus": [17741.3], "XYStage": [ 8775.300000000001, 32017.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos13", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.815], "Focus": [17741.3], "XYStage": [ 8109.200000000001, 32008.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos14", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.805], "Focus": [17741.3], "XYStage": [ 3483.3, 31908.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos15", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.82], "Focus": [17741.2], "XYStage": [ 2686.8, 31903.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos16", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [211.48000000000002], "Focus": [17741.1], "XYStage": [ -1978.9, 31973.600000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos17", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [211.51500000000001], "Focus": [17741.1], "XYStage": [ -2957.8, 31982.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos18", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.195], "Focus": [17741.5], "XYStage": [ 47747.4, -576.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos19", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.23000000000002], "Focus": [17741.6], "XYStage": [ 47053.9, -576.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos20", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.205], "Focus": [17741.4], "XYStage": [ 41871.200000000004, -479.8 ] }, "GridColumnIndex": 0 }, { "Label": "Pos21", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.4], "XYStage": [ 40882.600000000006, -392 ] }, "GridColumnIndex": 0 }, { "Label": "Pos22", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.24], "Focus": [17741.4], "XYStage": [ 35905.6, -135.3 ] }, "GridColumnIndex": 0 }, { "Label": "Pos23", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.195], "Focus": [17741.5], "XYStage": [ 34990.9, -132.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos24", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.46], "Focus": [17741.4], "XYStage": [ 29678.100000000002, -958.1 ] }, "GridColumnIndex": 0 }, { "Label": "Pos25", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.44], "Focus": [17741.5], "XYStage": [ 28983.600000000002, -939.4000000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos26", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.475], "Focus": [17741.5], "XYStage": [ 23019.7, -122 ] }, "GridColumnIndex": 0 }, { "Label": "Pos27", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.44], "Focus": [17741.7], "XYStage": [ 22428, -116.80000000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos28", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.46], "Focus": [17741.7], "XYStage": [ 16364.900000000001, -181.10000000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos29", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.48000000000002], "Focus": [17741.5], "XYStage": [ 15712.1, -180 ] }, "GridColumnIndex": 0 }, { "Label": "Pos30", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.45000000000002], "Focus": [17741.7], "XYStage": [ 10346.1, 77.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos31", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.49], "Focus": [17741.4], "XYStage": [ 9755.5, 85.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos32", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.475], "Focus": [17741.5], "XYStage": [ 4775.5, 293.90000000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos33", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.445], "Focus": [17741.4], "XYStage": [ 3954.9, 293.90000000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos34", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.455], "Focus": [17741.4], "XYStage": [ -1324.9, 545.6 ] }, "GridColumnIndex": 0 }, { "Label": "Pos35", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.445], "Focus": [17741.3], "XYStage": [ -2097, 576.6 ] }, "GridColumnIndex": 0 } ], "z-step_um": 10, "GridRow": 0, "BitDepth": 16, "Depth": 2, "Channels": 6, "Source": "Micro-Manager", "ChColors": [ -16711681, -256, -65281, -1, -1, -16776961 ], "MetadataVersion": 10, "KeepShutterOpenChannels": true, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536, 65536 ], "CustomIntervals_ms": [], "Height": 1200, "MicroManagerVersion": "1.4.23 20180219", "GridColumn": 0, "UUID": "67598d21-5db5-4842-ab60-f506f7b42c7c", "PixelType": "GRAY16", "Width": 1200, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\Yan\\20191218-YC113-S7S8-miseq", "Frames": 1, "Comment": "", "UserName": "Nikon", "PixelSize_um": 1.1, "ROI": [ 0, 0, 1200, 1200 ], "Date": "2019-12-18", "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC", "P-DAPI-high" ], "KeepShutterOpenSlices": true, "IJType": 1, "ComputerName": "ZadorConfocal2", "Positions": 36, "PositionIndex": 29, "CameraTimeout": 20000, "TimeFirst": false }, "640 AO-Name": "AnalogIO", "Vincent-D3-Command": "Close" }, "FrameKey-0-2-3": { "COM4-StopBits": "1", "528 AO-MaxVolts": "5.0000", "XLIGHT Excitation Wheel-State": "1", "470 Shutter-Name": "AnalogIO", "Core-Shutter": "640", "555 AO-Demo": "No", "XLIGHT Dichroic-State": "2", "XYStage-StepSize-Y": "0.1000", "ZPositionUm": 198.48000000000002, "XYStage-StepSize-X": "0.1000", "PRIME-FirmwareVersion": "20.28", "PRIME-ClearCycles": "2", "520-Name": "DA Shutter", "405 Shutter-Name": "AnalogIO", "PIEZO-StepSize": "0.0050", "PRIME-ClearMode": "Pre-Exposure", "470 AO-MinVolts": "0.0000", "405 AO-SupportsTriggering": "1", "CameraChannelIndex": 0, "XYStage-StartSpeed": "500.0000", "COM9-StopBits": "2", "405 AO-Description": "NI DAC", "COM4-DelayBetweenCharsMs": "0.0000", "445 AO-Block voltage": "false", "520 AO-TriggerInputLine": "/Dev1/PFI0", "555 AO-Description": "NI DAC", "520 AO-TriggerSampleRate": "100000", "COM4-Verbose": "1", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM4-Name": "COM4", "405 Shutter-Volts": "0.0000", "Vincent-D3-Port": "COM1", "PRIME-PP 4 MIN ADU AFFECTED": "200", "470 Shutter-MinVolts": "0.0000", "PRIME-PP 3 ENABLED": "Yes", "470 AO-Description": "NI DAC", "PRIME-Description": "PVCAM API device adapter", "520 Shutter-MaxVolts": "5.0000", "520 Shutter-Volts": "0.0000", "528 AO-Sequenceable": "1", "PRIME-Binning": "1x1", "555 Shutter-MaxVolts": "5.0000", "528 Shutter-TriggerSequenceLength": "1000", "405 Shutter-Block voltage": "false", "XLIGHT Spin motor-State": "1", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "Vincent-D3-Time to open (ms)": "35.0000", "Vincent-D3-Time to close (ms)": "35.0000", "445 AO-TriggerSampleRate": "100000", "640 Shutter-IOChannel": "Dev1/ao14", "555 AO-Sequenceable": "1", "520 AO-Name": "AnalogIO", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "470 AO-Block voltage": "false", "COM1-Verbose": "1", "COM9-AnswerTimeout": "10000.0000", "528 Shutter-Name": "AnalogIO", "PRIME-Trigger Timeout (secs)": "10", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "NextFrame": 0, "470 AO-TriggerSequenceLength": "1000", "PRIME-TransposeXY": "0", "445 AO-TriggerSequenceLength": "1000", "COM1-Name": "COM1", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "COM9-DataBits": "8", "COM1-AnswerTimeout": "500.0000", "Camera": "", "TE2000-Version": "V2.50-V2.50-V2.10", "528 Shutter-Volts": "0.0000", "XLIGHT Hub-Name": "XLIGHT Hub", "PIEZO-Description": "Ludl stage driver adapter", "PositionName": "Pos29", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSampleRate": "100000", "PRIME-FanSpeedSetpoint": "High", "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "405 AO-TriggerSampleRate": "100000", "470 Shutter-Block voltage": "false", "470-State": "0", "COM3-Parity": "None", "405 AO-Volts": "0.0000", "PRIME-TransposeMirrorY": "0", "PRIME-ChipName": "GS144BSI", "470 AO-Volts": "0.0000", "PRIME-TransposeMirrorX": "0", "445 Shutter-SupportsTriggering": "0", "470 Shutter-SupportsTriggering": "0", "XLIGHT Hub-Description": "XLIGHT hub", "PRIME-PreampOffLimit": "10000", "470 AO-Sequenceable": "1", "528 Shutter-MaxVolts": "5.0000", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "Core-XYStage": "XYStage", "470 AO-TriggerSampleRate": "100000", "PRIME-Timing-PreTriggerDelayNs": "24672000.0000", "528-DA Device": "528 Shutter", "Focus-Description": "Nikon TE2000 focus adapter", "FilterBlock-State": "5", "520 AO-Demo": "No", "COM3-DelayBetweenCharsMs": "0.0000", "470 Shutter-Volts": "0.0000", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "PRIME-PP 2 THRESHOLD": "125", "PRIME-PP 4 THRESHOLD": "75", "XYStage-Acceleration": "4.0000", "470 Shutter-Demo": "No", "PRIME-Name": "PMPCIECam00", "640-DA Device": "640 Shutter", "405 AO-Block voltage": "false", "ElapsedTime-ms": 1204529, "PRIME-Offset": "100", "445 AO-IOChannel": "Dev1/ao1", "640 AO-MaxVolts": "5.0000", "PositionIndex": 29, "PRIME-PP 1 ENABLED": "Yes", "COM1-BaudRate": "9600", "640-State": "1", "COM3-Verbose": "1", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "LudlController-TransmissionDelay": "0", "PRIME-FullWellCapacity": "62000", "PRIME-SMARTStreamingEnabled": "No", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "640 Shutter-Description": "NI DAC", "528-Description": "DA device that is used as a shutter", "COM4-Description": "Serial port driver (boost:asio)", "405 Shutter-Description": "NI DAC", "TE2000-Name": "TE2000", "528 Shutter-Description": "NI DAC", "COM9-Description": "Serial port driver (boost:asio)", "LudlController-Name": "LudlController", "445 Shutter-TriggerSequenceLength": "1000", "PRIME-SerialNumber": "A18B203008", "445 AO-Volts": "0.0000", "PRIME-X-dimension": "1200", "PRIME-ShutterCloseDelay": "0", "XLIGHT Disk slider-State": "2", "Position": "Default", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 AO-IOChannel": "Dev1/ao5", "520 AO-IOChannel": "Dev1/ao4", "528 AO-TriggerSequenceLength": "1000", "405 Shutter-MaxVolts": "5.0000", "470-DA Device": "470 Shutter", "PRIME-FTCapable": "No", "Core-ChannelGroup": "Channels", "555 AO-SupportsTriggering": "0", "PRIME-Y-dimension": "1200", "Frame": 0, "555 Shutter-Sequenceable": "1", "PRIME-Timing-ExposureTimeNs": "499998000.0000", "Core-ImageProcessor": "", "528-State": "0", "520 AO-Block voltage": "false", "XLIGHT Excitation Wheel-Label": "QUAD", "640 Shutter-Demo": "No", "COM3-StopBits": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "520 Shutter-IOChannel": "Dev1/ao11", "Width": 1200, "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-SupportsTriggering": "0", "640 AO-TriggerSampleRate": "100000", "520-Description": "DA device that is used as a shutter", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "405 Shutter-Sequenceable": "1", "405 Shutter-SupportsTriggering": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-TriggerSampleRate": "100000", "640 Shutter-MaxVolts": "5.0000", "555 AO-MaxVolts": "5.0000", "520 AO-TriggerSequenceLength": "1000", "COM3-BaudRate": "9600", "COM9-BaudRate": "9600", "Vincent-D3-Channel #": "Ch. #1", "PRIME-PMode": "Normal", "OpticalPath-Label": "Left(100)", "BitDepth": 16, "555 AO-TriggerSequenceLength": "1000", "445 Shutter-Description": "NI DAC", "405 AO-Sequenceable": "1", "PRIME-AcquisitionMethod": "Callbacks", "555 AO-Name": "AnalogIO", "PRIME-ShutterMode": "Pre-Exposure", "COM3-Handshaking": "Off", "445 AO-SupportsTriggering": "0", "ROI": "0-0-1200-1200", "COM9-Verbose": "1", "PRIME-Color": "OFF", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "PRIME-ShutterOpenDelay": "0", "COM1-Parity": "None", "Slice": 3, "SlicePosition": -20, "COM9-DelayBetweenCharsMs": "0.0000", "555-Name": "DA Shutter", "PIEZO-LudlSingleAxisName": "B", "XLIGHT Emission Wheel-Label": "679/29 (A)", "640 Shutter-Name": "AnalogIO", "640 AO-Sequenceable": "1", "520 Shutter-Demo": "No", "528 Shutter-Demo": "No", "470-Name": "DA Shutter", "520-DA Device": "520 Shutter", "XYStage-Description": "Ludl XY stage driver adapter", "445 Shutter-MinVolts": "0.0000", "528 AO-TriggerSampleRate": "100000", "405 AO-MinVolts": "0.0000", "405 Shutter-TriggerSequenceLength": "1000", "Height": 1200, "555 AO-Block voltage": "false", "640 Shutter-Volts": "5.0000", "PixelType": "GRAY16", "445 Shutter-Block voltage": "false", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "520 Shutter-TriggerSequenceLength": "1000", "COM9-Name": "COM9", "Core-SLM": "", "555 AO-Volts": "0.0000", "555 Shutter-Block voltage": "false", "XYStage-Speed": "1800.0000", "520 AO-MaxVolts": "5.0000", "528 Shutter-SupportsTriggering": "0", "COM1-Description": "Serial port driver (boost:asio)", "PRIME-MetadataEnabled": "No", "520 Shutter-MinVolts": "0.0000", "470 Shutter-Sequenceable": "1", "PRIME-PreampDelay": "0", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-MinVolts": "0.0000", "ChannelIndex": 2, "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Core-AutoShutter": "1", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "Exposure-ms": 320, "PRIME-PP 2 ENABLED": "Yes", "640 Shutter-TriggerSampleRate": "100000", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "470 Shutter-IOChannel": "Dev1/ao10", "405 AO-Demo": "No", "PixelSizeUm": 1.1, "470 AO-Demo": "No", "470 Shutter-MaxVolts": "5.0000", "Vincent-D3-Name": "Vincent-D3", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FileName": "img_000000000_Il-A_003.tif", "LudlController-Reset": "Operate", "PRIME-Exposure": "320.00", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "445-Name": "DA Shutter", "555 Shutter-Name": "AnalogIO", "520 Shutter-TriggerSampleRate": "100000", "445 Shutter-IOChannel": "Dev1/ao9", "PRIME-TransposeCorrection": "0", "XYStage-StepSize": "0.1000", "445-Description": "DA device that is used as a shutter", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 AO-IOChannel": "Dev1/ao7", "520 Shutter-Name": "AnalogIO", "COM3-DataBits": "8", "640 AO-Volts": "4.5000", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "555 Shutter-IOChannel": "Dev1/ao13", "WaitInterval": null, "520 Shutter-Sequenceable": "1", "YPositionUm": -180, "445-State": "0", "445-DA Device": "445 Shutter", "PRIME-CircularBufferAutoSize": "ON", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "555 AO-TriggerSampleRate": "100000", "445 Shutter-Volts": "0.0000", "XLIGHT Hub-Port": "COM3", "Core-AutoFocus": "", "COM4-Handshaking": "Off", "528 Shutter-MinVolts": "0.0000", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "445 Shutter-Name": "AnalogIO", "528 Shutter-Sequenceable": "1", "XYStage-TransposeMirrorX": "0", "XYStage-TransposeMirrorY": "0", "PRIME-ReadoutRate": "100MHz 16bit", "XYStage-Name": "Stage", "640 AO-SupportsTriggering": "0", "640-Name": "DA Shutter", "640 Shutter-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "640 AO-Block voltage": "false", "445 Shutter-Sequenceable": "1", "470 AO-MaxVolts": "5.0000", "COM4-BaudRate": "9600", "470-Description": "DA device that is used as a shutter", "520 Shutter-Description": "NI DAC", "PRIME-CCDTemperature": "-14.8500", "Time": "2019-12-18 23:11:04 -0500", "640 AO-TriggerSequenceLength": "1000", "470 AO-Name": "AnalogIO", "XLIGHT Disk slider-Label": "Disk Pos 40um", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "Binning": "1x1", "TE2000-ModelType": "1", "COM3-AnswerTimeout": "500.0000", "555 Shutter-SupportsTriggering": "0", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "TE2000-Port": "COM4", "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Description": "NI DAC", "COM4-AnswerTimeout": "500.0000", "COM1-DelayBetweenCharsMs": "0.0000", "FilterBlock-Label": "Confocal", "445 AO-Name": "AnalogIO", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-Timing-ReadoutTimeNs": "24672000.0000", "528 AO-Demo": "No", "520 AO-Sequenceable": "1", "555-Description": "DA device that is used as a shutter", "405-DA Device": "405 Shutter", "405 AO-TriggerSequenceLength": "1000", "640 AO-MinVolts": "0.0000", "640 Shutter-Block voltage": "false", "FrameIndex": 0, "PRIME-Port": "CMOS", "555 AO-MinVolts": "0.0000", "COM3-Description": "Serial port driver (boost:asio)", "528 AO-Description": "NI DAC", "555 AO-IOChannel": "Dev1/ao6", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "555 AO-TriggerInputLine": "/Dev1/PFI0", "PRIME-ActualInterval-ms": "390.5200", "LudlController-Port": "COM9", "445 Shutter-Demo": "No", "PRIME-Trigger-Expose Out-Mux": "1", "SliceIndex": 3, "PRIME-CCDTemperatureSetPoint": "-15.0000", "405 Shutter-MinVolts": "0.0000", "PRIME-ExposeOutMode": "First Row", "AxisPositions": null, "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Volts": "0.0000", "528-Name": "DA Shutter", "555-State": "0", "XLIGHT Dichroic-Label": "Di03-R635-t1", "445 AO-Sequenceable": "1", "405-Name": "DA Shutter", "405 AO-IOChannel": "Dev1/ao0", "Core-Camera": "PRIME", "Focus-Name": "Focus", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "405 Shutter-IOChannel": "Dev1/ao8", "640 AO-Demo": "No", "640 Shutter-SupportsTriggering": "0", "445 AO-Demo": "No", "555-DA Device": "555 Shutter", "470 Shutter-Description": "NI DAC", "528 AO-Volts": "0.0000", "COM1-DataBits": "8", "Vincent-D3-Address": "x", "520 AO-SupportsTriggering": "0", "405-Description": "DA device that is used as a shutter", "640 Shutter-MinVolts": "0.0000", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "405 AO-Name": "AnalogIO", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "640 AO-TriggerInputLine": "/Dev1/PFI0", "405 AO-TriggerInputLine": "/Dev1/PFI0", "640-Description": "DA device that is used as a shutter", "445 Shutter-TriggerSampleRate": "100000", "470 Shutter-TriggerSequenceLength": "1000", "COM1-StopBits": "1", "520 Shutter-SupportsTriggering": "0", "405-State": "0", "Core-Initialize": "1", "XLIGHT Emission Wheel-State": "3", "520 AO-Description": "NI DAC", "528 AO-TriggerInputLine": "/Dev1/PFI0", "445 Shutter-MaxVolts": "5.0000", "COM9-Handshaking": "Off", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "XLIGHT Spin motor-Label": "On (spinning)", "PRIME-PP 5 ENABLED": "No", "UUID": "9be16d27-c818-4a0e-90c3-2df9ddc6cdfa", "PRIME-TriggerMode": "Internal Trigger", "COM1-Handshaking": "Off", "PRIME-Timing-PostTriggerDelayNs": "49344000.0000", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "Focus-Position": "17741.5000", "528 Shutter-Block voltage": "false", "Channel": "Il-A", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "555 Shutter-Demo": "No", "405 Shutter-Demo": "No", "528 AO-Name": "AnalogIO", "520-State": "0", "Core-Galvo": "", "405 Shutter-TriggerSampleRate": "100000", "Focus-FocusStepSize_(nm)": "100", "PRIME-PixelType": "16bit", "COM4-DataBits": "8", "470 AO-IOChannel": "Dev1/ao2", "520 AO-MinVolts": "0.0000", "COM9-Parity": "None", "PRIME-CircularBufferEnabled": "ON", "PRIME-PP 6 Reset": "No", "640 AO-Description": "NI DAC", "Source": "PRIME", "445 AO-MinVolts": "0.0000", "PIEZO-Name": "Stage", "445 AO-Description": "NI DAC", "PRIME-CircularBufferFrameCount": "46", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "520 AO-Volts": "0.0000", "528 Shutter-TriggerSampleRate": "100000", "XPositionUm": 15712.1, "OpticalPath-State": "4", "PRIME-PP 3 THRESHOLD": "80", "PRIME-PVCAM Version": "3.7.1", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Gain": "1-HDR", "640 Shutter-TriggerSequenceLength": "1000", "528 AO-SupportsTriggering": "0", "445 AO-TriggerInputLine": "/Dev1/PFI0", "OpticalPath-Name": "OpticalPath", "PRIME-PP 4 ENABLED": "Yes", "PRIME-PVCAM Adapter Version": "1.3.29", "COM3-Name": "COM3", "405 AO-MaxVolts": "5.0000", "520 Shutter-Block voltage": "false", "PRIME-Timing-ClearingTimeNs": "49344000.0000", "Core-Focus": "PIEZO", "528 AO-Block voltage": "false", "TE2000-Description": "Nikon TE2000 hub", "528 AO-MinVolts": "0.0000", "COM4-Parity": "None", "Summary": { "Slices": 11, "ChContrastMin": [ 0, 0, 0, 0, 0, 0 ], "PixelAspect": 1, "SlicesFirst": true, "Interval_ms": 0, "Time": "2019-12-18 23:10:50 -0500", "Prefix": "seq_5", "InitialPositionList": [ { "Label": "Pos0", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.21], "Focus": [17741.5], "XYStage": [ 44542.200000000004, 31636.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos1", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.22], "Focus": [17741.4], "XYStage": [ 43801.100000000006, 31637.9 ] }, "GridColumnIndex": 0 }, { "Label": "Pos2", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.5], "XYStage": [ 38561.3, 32040.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos3", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.185], "Focus": [17741.3], "XYStage": [ 37698.4, 32041.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos4", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.16], "Focus": [17741.5], "XYStage": [ 32375.4, 31758.9 ] }, "GridColumnIndex": 0 }, { "Label": "Pos5", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.17000000000002], "Focus": [17741.3], "XYStage": [ 31770.7, 31758.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos6", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.4], "XYStage": [ 26536.800000000003, 31068.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos7", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.205], "Focus": [17741.4], "XYStage": [ 25420.2, 31068.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos8", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.85], "Focus": [17741.3], "XYStage": [ 21059, 31508 ] }, "GridColumnIndex": 0 }, { "Label": "Pos9", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.82], "Focus": [17741.3], "XYStage": [ 19731.9, 31506.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos10", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.79], "Focus": [17741.4], "XYStage": [ 14528.5, 32007.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos11", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.83], "Focus": [17741.2], "XYStage": [ 13830.300000000001, 32009.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos12", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.79], "Focus": [17741.3], "XYStage": [ 8775.300000000001, 32017.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos13", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.815], "Focus": [17741.3], "XYStage": [ 8109.200000000001, 32008.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos14", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.805], "Focus": [17741.3], "XYStage": [ 3483.3, 31908.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos15", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.82], "Focus": [17741.2], "XYStage": [ 2686.8, 31903.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos16", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [211.48000000000002], "Focus": [17741.1], "XYStage": [ -1978.9, 31973.600000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos17", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [211.51500000000001], "Focus": [17741.1], "XYStage": [ -2957.8, 31982.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos18", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.195], "Focus": [17741.5], "XYStage": [ 47747.4, -576.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos19", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.23000000000002], "Focus": [17741.6], "XYStage": [ 47053.9, -576.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos20", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.205], "Focus": [17741.4], "XYStage": [ 41871.200000000004, -479.8 ] }, "GridColumnIndex": 0 }, { "Label": "Pos21", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.4], "XYStage": [ 40882.600000000006, -392 ] }, "GridColumnIndex": 0 }, { "Label": "Pos22", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.24], "Focus": [17741.4], "XYStage": [ 35905.6, -135.3 ] }, "GridColumnIndex": 0 }, { "Label": "Pos23", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.195], "Focus": [17741.5], "XYStage": [ 34990.9, -132.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos24", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.46], "Focus": [17741.4], "XYStage": [ 29678.100000000002, -958.1 ] }, "GridColumnIndex": 0 }, { "Label": "Pos25", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.44], "Focus": [17741.5], "XYStage": [ 28983.600000000002, -939.4000000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos26", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.475], "Focus": [17741.5], "XYStage": [ 23019.7, -122 ] }, "GridColumnIndex": 0 }, { "Label": "Pos27", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.44], "Focus": [17741.7], "XYStage": [ 22428, -116.80000000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos28", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.46], "Focus": [17741.7], "XYStage": [ 16364.900000000001, -181.10000000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos29", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.48000000000002], "Focus": [17741.5], "XYStage": [ 15712.1, -180 ] }, "GridColumnIndex": 0 }, { "Label": "Pos30", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.45000000000002], "Focus": [17741.7], "XYStage": [ 10346.1, 77.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos31", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.49], "Focus": [17741.4], "XYStage": [ 9755.5, 85.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos32", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.475], "Focus": [17741.5], "XYStage": [ 4775.5, 293.90000000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos33", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.445], "Focus": [17741.4], "XYStage": [ 3954.9, 293.90000000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos34", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.455], "Focus": [17741.4], "XYStage": [ -1324.9, 545.6 ] }, "GridColumnIndex": 0 }, { "Label": "Pos35", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.445], "Focus": [17741.3], "XYStage": [ -2097, 576.6 ] }, "GridColumnIndex": 0 } ], "z-step_um": 10, "GridRow": 0, "BitDepth": 16, "Depth": 2, "Channels": 6, "Source": "Micro-Manager", "ChColors": [ -16711681, -256, -65281, -1, -1, -16776961 ], "MetadataVersion": 10, "KeepShutterOpenChannels": true, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536, 65536 ], "CustomIntervals_ms": [], "Height": 1200, "MicroManagerVersion": "1.4.23 20180219", "GridColumn": 0, "UUID": "67598d21-5db5-4842-ab60-f506f7b42c7c", "PixelType": "GRAY16", "Width": 1200, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\Yan\\20191218-YC113-S7S8-miseq", "Frames": 1, "Comment": "", "UserName": "Nikon", "PixelSize_um": 1.1, "ROI": [ 0, 0, 1200, 1200 ], "Date": "2019-12-18", "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC", "P-DAPI-high" ], "KeepShutterOpenSlices": true, "IJType": 1, "ComputerName": "ZadorConfocal2", "Positions": 36, "PositionIndex": 29, "CameraTimeout": 20000, "TimeFirst": false }, "640 AO-Name": "AnalogIO", "Vincent-D3-Command": "Close" }, "FrameKey-0-2-4": { "COM4-StopBits": "1", "528 AO-MaxVolts": "5.0000", "XLIGHT Excitation Wheel-State": "1", "470 Shutter-Name": "AnalogIO", "Core-Shutter": "640", "555 AO-Demo": "No", "XLIGHT Dichroic-State": "2", "XYStage-StepSize-Y": "0.1000", "ZPositionUm": 208.48000000000002, "XYStage-StepSize-X": "0.1000", "PRIME-FirmwareVersion": "20.28", "PRIME-ClearCycles": "2", "520-Name": "DA Shutter", "405 Shutter-Name": "AnalogIO", "PIEZO-StepSize": "0.0050", "PRIME-ClearMode": "Pre-Exposure", "470 AO-MinVolts": "0.0000", "405 AO-SupportsTriggering": "1", "CameraChannelIndex": 0, "XYStage-StartSpeed": "500.0000", "COM9-StopBits": "2", "405 AO-Description": "NI DAC", "COM4-DelayBetweenCharsMs": "0.0000", "445 AO-Block voltage": "false", "520 AO-TriggerInputLine": "/Dev1/PFI0", "555 AO-Description": "NI DAC", "520 AO-TriggerSampleRate": "100000", "COM4-Verbose": "1", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM4-Name": "COM4", "405 Shutter-Volts": "0.0000", "Vincent-D3-Port": "COM1", "PRIME-PP 4 MIN ADU AFFECTED": "200", "470 Shutter-MinVolts": "0.0000", "PRIME-PP 3 ENABLED": "Yes", "470 AO-Description": "NI DAC", "PRIME-Description": "PVCAM API device adapter", "520 Shutter-MaxVolts": "5.0000", "520 Shutter-Volts": "0.0000", "528 AO-Sequenceable": "1", "PRIME-Binning": "1x1", "555 Shutter-MaxVolts": "5.0000", "528 Shutter-TriggerSequenceLength": "1000", "405 Shutter-Block voltage": "false", "XLIGHT Spin motor-State": "1", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "Vincent-D3-Time to open (ms)": "35.0000", "Vincent-D3-Time to close (ms)": "35.0000", "445 AO-TriggerSampleRate": "100000", "640 Shutter-IOChannel": "Dev1/ao14", "555 AO-Sequenceable": "1", "520 AO-Name": "AnalogIO", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "470 AO-Block voltage": "false", "COM1-Verbose": "1", "COM9-AnswerTimeout": "10000.0000", "528 Shutter-Name": "AnalogIO", "PRIME-Trigger Timeout (secs)": "10", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "NextFrame": 0, "470 AO-TriggerSequenceLength": "1000", "PRIME-TransposeXY": "0", "445 AO-TriggerSequenceLength": "1000", "COM1-Name": "COM1", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "COM9-DataBits": "8", "COM1-AnswerTimeout": "500.0000", "Camera": "", "TE2000-Version": "V2.50-V2.50-V2.10", "528 Shutter-Volts": "0.0000", "XLIGHT Hub-Name": "XLIGHT Hub", "PIEZO-Description": "Ludl stage driver adapter", "PositionName": "Pos29", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSampleRate": "100000", "PRIME-FanSpeedSetpoint": "High", "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "405 AO-TriggerSampleRate": "100000", "470 Shutter-Block voltage": "false", "470-State": "0", "COM3-Parity": "None", "405 AO-Volts": "0.0000", "PRIME-TransposeMirrorY": "0", "PRIME-ChipName": "GS144BSI", "470 AO-Volts": "0.0000", "PRIME-TransposeMirrorX": "0", "445 Shutter-SupportsTriggering": "0", "470 Shutter-SupportsTriggering": "0", "XLIGHT Hub-Description": "XLIGHT hub", "PRIME-PreampOffLimit": "10000", "470 AO-Sequenceable": "1", "528 Shutter-MaxVolts": "5.0000", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "Core-XYStage": "XYStage", "470 AO-TriggerSampleRate": "100000", "PRIME-Timing-PreTriggerDelayNs": "24672000.0000", "528-DA Device": "528 Shutter", "Focus-Description": "Nikon TE2000 focus adapter", "FilterBlock-State": "5", "520 AO-Demo": "No", "COM3-DelayBetweenCharsMs": "0.0000", "470 Shutter-Volts": "0.0000", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "PRIME-PP 2 THRESHOLD": "125", "PRIME-PP 4 THRESHOLD": "75", "XYStage-Acceleration": "4.0000", "470 Shutter-Demo": "No", "PRIME-Name": "PMPCIECam00", "640-DA Device": "640 Shutter", "405 AO-Block voltage": "false", "ElapsedTime-ms": 1205086, "PRIME-Offset": "100", "445 AO-IOChannel": "Dev1/ao1", "640 AO-MaxVolts": "5.0000", "PositionIndex": 29, "PRIME-PP 1 ENABLED": "Yes", "COM1-BaudRate": "9600", "640-State": "1", "COM3-Verbose": "1", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "LudlController-TransmissionDelay": "0", "PRIME-FullWellCapacity": "62000", "PRIME-SMARTStreamingEnabled": "No", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "640 Shutter-Description": "NI DAC", "528-Description": "DA device that is used as a shutter", "COM4-Description": "Serial port driver (boost:asio)", "405 Shutter-Description": "NI DAC", "TE2000-Name": "TE2000", "528 Shutter-Description": "NI DAC", "COM9-Description": "Serial port driver (boost:asio)", "LudlController-Name": "LudlController", "445 Shutter-TriggerSequenceLength": "1000", "PRIME-SerialNumber": "A18B203008", "445 AO-Volts": "0.0000", "PRIME-X-dimension": "1200", "PRIME-ShutterCloseDelay": "0", "XLIGHT Disk slider-State": "2", "Position": "Default", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 AO-IOChannel": "Dev1/ao5", "520 AO-IOChannel": "Dev1/ao4", "528 AO-TriggerSequenceLength": "1000", "405 Shutter-MaxVolts": "5.0000", "470-DA Device": "470 Shutter", "PRIME-FTCapable": "No", "Core-ChannelGroup": "Channels", "555 AO-SupportsTriggering": "0", "PRIME-Y-dimension": "1200", "Frame": 0, "555 Shutter-Sequenceable": "1", "PRIME-Timing-ExposureTimeNs": "499998000.0000", "Core-ImageProcessor": "", "528-State": "0", "520 AO-Block voltage": "false", "XLIGHT Excitation Wheel-Label": "QUAD", "640 Shutter-Demo": "No", "COM3-StopBits": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "520 Shutter-IOChannel": "Dev1/ao11", "Width": 1200, "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-SupportsTriggering": "0", "640 AO-TriggerSampleRate": "100000", "520-Description": "DA device that is used as a shutter", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "405 Shutter-Sequenceable": "1", "405 Shutter-SupportsTriggering": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-TriggerSampleRate": "100000", "640 Shutter-MaxVolts": "5.0000", "555 AO-MaxVolts": "5.0000", "520 AO-TriggerSequenceLength": "1000", "COM3-BaudRate": "9600", "COM9-BaudRate": "9600", "Vincent-D3-Channel #": "Ch. #1", "PRIME-PMode": "Normal", "OpticalPath-Label": "Left(100)", "BitDepth": 16, "555 AO-TriggerSequenceLength": "1000", "445 Shutter-Description": "NI DAC", "405 AO-Sequenceable": "1", "PRIME-AcquisitionMethod": "Callbacks", "555 AO-Name": "AnalogIO", "PRIME-ShutterMode": "Pre-Exposure", "COM3-Handshaking": "Off", "445 AO-SupportsTriggering": "0", "ROI": "0-0-1200-1200", "COM9-Verbose": "1", "PRIME-Color": "OFF", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "PRIME-ShutterOpenDelay": "0", "COM1-Parity": "None", "Slice": 4, "SlicePosition": -10, "COM9-DelayBetweenCharsMs": "0.0000", "555-Name": "DA Shutter", "PIEZO-LudlSingleAxisName": "B", "XLIGHT Emission Wheel-Label": "679/29 (A)", "640 Shutter-Name": "AnalogIO", "640 AO-Sequenceable": "1", "520 Shutter-Demo": "No", "528 Shutter-Demo": "No", "470-Name": "DA Shutter", "520-DA Device": "520 Shutter", "XYStage-Description": "Ludl XY stage driver adapter", "445 Shutter-MinVolts": "0.0000", "528 AO-TriggerSampleRate": "100000", "405 AO-MinVolts": "0.0000", "405 Shutter-TriggerSequenceLength": "1000", "Height": 1200, "555 AO-Block voltage": "false", "640 Shutter-Volts": "5.0000", "PixelType": "GRAY16", "445 Shutter-Block voltage": "false", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "520 Shutter-TriggerSequenceLength": "1000", "COM9-Name": "COM9", "Core-SLM": "", "555 AO-Volts": "0.0000", "555 Shutter-Block voltage": "false", "XYStage-Speed": "1800.0000", "520 AO-MaxVolts": "5.0000", "528 Shutter-SupportsTriggering": "0", "COM1-Description": "Serial port driver (boost:asio)", "PRIME-MetadataEnabled": "No", "520 Shutter-MinVolts": "0.0000", "470 Shutter-Sequenceable": "1", "PRIME-PreampDelay": "0", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-MinVolts": "0.0000", "ChannelIndex": 2, "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Core-AutoShutter": "1", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "Exposure-ms": 320, "PRIME-PP 2 ENABLED": "Yes", "640 Shutter-TriggerSampleRate": "100000", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "470 Shutter-IOChannel": "Dev1/ao10", "405 AO-Demo": "No", "PixelSizeUm": 1.1, "470 AO-Demo": "No", "470 Shutter-MaxVolts": "5.0000", "Vincent-D3-Name": "Vincent-D3", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FileName": "img_000000000_Il-A_004.tif", "LudlController-Reset": "Operate", "PRIME-Exposure": "320.00", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "445-Name": "DA Shutter", "555 Shutter-Name": "AnalogIO", "520 Shutter-TriggerSampleRate": "100000", "445 Shutter-IOChannel": "Dev1/ao9", "PRIME-TransposeCorrection": "0", "XYStage-StepSize": "0.1000", "445-Description": "DA device that is used as a shutter", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 AO-IOChannel": "Dev1/ao7", "520 Shutter-Name": "AnalogIO", "COM3-DataBits": "8", "640 AO-Volts": "4.5000", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "555 Shutter-IOChannel": "Dev1/ao13", "WaitInterval": null, "520 Shutter-Sequenceable": "1", "YPositionUm": -180, "445-State": "0", "445-DA Device": "445 Shutter", "PRIME-CircularBufferAutoSize": "ON", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "555 AO-TriggerSampleRate": "100000", "445 Shutter-Volts": "0.0000", "XLIGHT Hub-Port": "COM3", "Core-AutoFocus": "", "COM4-Handshaking": "Off", "528 Shutter-MinVolts": "0.0000", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "445 Shutter-Name": "AnalogIO", "528 Shutter-Sequenceable": "1", "XYStage-TransposeMirrorX": "0", "XYStage-TransposeMirrorY": "0", "PRIME-ReadoutRate": "100MHz 16bit", "XYStage-Name": "Stage", "640 AO-SupportsTriggering": "0", "640-Name": "DA Shutter", "640 Shutter-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "640 AO-Block voltage": "false", "445 Shutter-Sequenceable": "1", "470 AO-MaxVolts": "5.0000", "COM4-BaudRate": "9600", "470-Description": "DA device that is used as a shutter", "520 Shutter-Description": "NI DAC", "PRIME-CCDTemperature": "-14.8500", "Time": "2019-12-18 23:11:05 -0500", "640 AO-TriggerSequenceLength": "1000", "470 AO-Name": "AnalogIO", "XLIGHT Disk slider-Label": "Disk Pos 40um", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "Binning": "1x1", "TE2000-ModelType": "1", "COM3-AnswerTimeout": "500.0000", "555 Shutter-SupportsTriggering": "0", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "TE2000-Port": "COM4", "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Description": "NI DAC", "COM4-AnswerTimeout": "500.0000", "COM1-DelayBetweenCharsMs": "0.0000", "FilterBlock-Label": "Confocal", "445 AO-Name": "AnalogIO", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-Timing-ReadoutTimeNs": "24672000.0000", "528 AO-Demo": "No", "520 AO-Sequenceable": "1", "555-Description": "DA device that is used as a shutter", "405-DA Device": "405 Shutter", "405 AO-TriggerSequenceLength": "1000", "640 AO-MinVolts": "0.0000", "640 Shutter-Block voltage": "false", "FrameIndex": 0, "PRIME-Port": "CMOS", "555 AO-MinVolts": "0.0000", "COM3-Description": "Serial port driver (boost:asio)", "528 AO-Description": "NI DAC", "555 AO-IOChannel": "Dev1/ao6", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "555 AO-TriggerInputLine": "/Dev1/PFI0", "PRIME-ActualInterval-ms": "390.5200", "LudlController-Port": "COM9", "445 Shutter-Demo": "No", "PRIME-Trigger-Expose Out-Mux": "1", "SliceIndex": 4, "PRIME-CCDTemperatureSetPoint": "-15.0000", "405 Shutter-MinVolts": "0.0000", "PRIME-ExposeOutMode": "First Row", "AxisPositions": null, "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Volts": "0.0000", "528-Name": "DA Shutter", "555-State": "0", "XLIGHT Dichroic-Label": "Di03-R635-t1", "445 AO-Sequenceable": "1", "405-Name": "DA Shutter", "405 AO-IOChannel": "Dev1/ao0", "Core-Camera": "PRIME", "Focus-Name": "Focus", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "405 Shutter-IOChannel": "Dev1/ao8", "640 AO-Demo": "No", "640 Shutter-SupportsTriggering": "0", "445 AO-Demo": "No", "555-DA Device": "555 Shutter", "470 Shutter-Description": "NI DAC", "528 AO-Volts": "0.0000", "COM1-DataBits": "8", "Vincent-D3-Address": "x", "520 AO-SupportsTriggering": "0", "405-Description": "DA device that is used as a shutter", "640 Shutter-MinVolts": "0.0000", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "405 AO-Name": "AnalogIO", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "640 AO-TriggerInputLine": "/Dev1/PFI0", "405 AO-TriggerInputLine": "/Dev1/PFI0", "640-Description": "DA device that is used as a shutter", "445 Shutter-TriggerSampleRate": "100000", "470 Shutter-TriggerSequenceLength": "1000", "COM1-StopBits": "1", "520 Shutter-SupportsTriggering": "0", "405-State": "0", "Core-Initialize": "1", "XLIGHT Emission Wheel-State": "3", "520 AO-Description": "NI DAC", "528 AO-TriggerInputLine": "/Dev1/PFI0", "445 Shutter-MaxVolts": "5.0000", "COM9-Handshaking": "Off", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "XLIGHT Spin motor-Label": "On (spinning)", "PRIME-PP 5 ENABLED": "No", "UUID": "d629111f-a1ac-4c45-8609-dbbac62ad548", "PRIME-TriggerMode": "Internal Trigger", "COM1-Handshaking": "Off", "PRIME-Timing-PostTriggerDelayNs": "49344000.0000", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "Focus-Position": "17741.5000", "528 Shutter-Block voltage": "false", "Channel": "Il-A", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "555 Shutter-Demo": "No", "405 Shutter-Demo": "No", "528 AO-Name": "AnalogIO", "520-State": "0", "Core-Galvo": "", "405 Shutter-TriggerSampleRate": "100000", "Focus-FocusStepSize_(nm)": "100", "PRIME-PixelType": "16bit", "COM4-DataBits": "8", "470 AO-IOChannel": "Dev1/ao2", "520 AO-MinVolts": "0.0000", "COM9-Parity": "None", "PRIME-CircularBufferEnabled": "ON", "PRIME-PP 6 Reset": "No", "640 AO-Description": "NI DAC", "Source": "PRIME", "445 AO-MinVolts": "0.0000", "PIEZO-Name": "Stage", "445 AO-Description": "NI DAC", "PRIME-CircularBufferFrameCount": "46", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "520 AO-Volts": "0.0000", "528 Shutter-TriggerSampleRate": "100000", "XPositionUm": 15712.1, "OpticalPath-State": "4", "PRIME-PP 3 THRESHOLD": "80", "PRIME-PVCAM Version": "3.7.1", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Gain": "1-HDR", "640 Shutter-TriggerSequenceLength": "1000", "528 AO-SupportsTriggering": "0", "445 AO-TriggerInputLine": "/Dev1/PFI0", "OpticalPath-Name": "OpticalPath", "PRIME-PP 4 ENABLED": "Yes", "PRIME-PVCAM Adapter Version": "1.3.29", "COM3-Name": "COM3", "405 AO-MaxVolts": "5.0000", "520 Shutter-Block voltage": "false", "PRIME-Timing-ClearingTimeNs": "49344000.0000", "Core-Focus": "PIEZO", "528 AO-Block voltage": "false", "TE2000-Description": "Nikon TE2000 hub", "528 AO-MinVolts": "0.0000", "COM4-Parity": "None", "Summary": { "Slices": 11, "ChContrastMin": [ 0, 0, 0, 0, 0, 0 ], "PixelAspect": 1, "SlicesFirst": true, "Interval_ms": 0, "Time": "2019-12-18 23:10:50 -0500", "Prefix": "seq_5", "InitialPositionList": [ { "Label": "Pos0", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.21], "Focus": [17741.5], "XYStage": [ 44542.200000000004, 31636.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos1", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.22], "Focus": [17741.4], "XYStage": [ 43801.100000000006, 31637.9 ] }, "GridColumnIndex": 0 }, { "Label": "Pos2", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.5], "XYStage": [ 38561.3, 32040.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos3", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.185], "Focus": [17741.3], "XYStage": [ 37698.4, 32041.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos4", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.16], "Focus": [17741.5], "XYStage": [ 32375.4, 31758.9 ] }, "GridColumnIndex": 0 }, { "Label": "Pos5", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.17000000000002], "Focus": [17741.3], "XYStage": [ 31770.7, 31758.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos6", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.4], "XYStage": [ 26536.800000000003, 31068.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos7", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.205], "Focus": [17741.4], "XYStage": [ 25420.2, 31068.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos8", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.85], "Focus": [17741.3], "XYStage": [ 21059, 31508 ] }, "GridColumnIndex": 0 }, { "Label": "Pos9", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.82], "Focus": [17741.3], "XYStage": [ 19731.9, 31506.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos10", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.79], "Focus": [17741.4], "XYStage": [ 14528.5, 32007.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos11", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.83], "Focus": [17741.2], "XYStage": [ 13830.300000000001, 32009.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos12", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.79], "Focus": [17741.3], "XYStage": [ 8775.300000000001, 32017.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos13", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.815], "Focus": [17741.3], "XYStage": [ 8109.200000000001, 32008.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos14", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.805], "Focus": [17741.3], "XYStage": [ 3483.3, 31908.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos15", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.82], "Focus": [17741.2], "XYStage": [ 2686.8, 31903.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos16", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [211.48000000000002], "Focus": [17741.1], "XYStage": [ -1978.9, 31973.600000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos17", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [211.51500000000001], "Focus": [17741.1], "XYStage": [ -2957.8, 31982.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos18", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.195], "Focus": [17741.5], "XYStage": [ 47747.4, -576.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos19", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.23000000000002], "Focus": [17741.6], "XYStage": [ 47053.9, -576.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos20", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.205], "Focus": [17741.4], "XYStage": [ 41871.200000000004, -479.8 ] }, "GridColumnIndex": 0 }, { "Label": "Pos21", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.4], "XYStage": [ 40882.600000000006, -392 ] }, "GridColumnIndex": 0 }, { "Label": "Pos22", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.24], "Focus": [17741.4], "XYStage": [ 35905.6, -135.3 ] }, "GridColumnIndex": 0 }, { "Label": "Pos23", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.195], "Focus": [17741.5], "XYStage": [ 34990.9, -132.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos24", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.46], "Focus": [17741.4], "XYStage": [ 29678.100000000002, -958.1 ] }, "GridColumnIndex": 0 }, { "Label": "Pos25", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.44], "Focus": [17741.5], "XYStage": [ 28983.600000000002, -939.4000000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos26", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.475], "Focus": [17741.5], "XYStage": [ 23019.7, -122 ] }, "GridColumnIndex": 0 }, { "Label": "Pos27", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.44], "Focus": [17741.7], "XYStage": [ 22428, -116.80000000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos28", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.46], "Focus": [17741.7], "XYStage": [ 16364.900000000001, -181.10000000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos29", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.48000000000002], "Focus": [17741.5], "XYStage": [ 15712.1, -180 ] }, "GridColumnIndex": 0 }, { "Label": "Pos30", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.45000000000002], "Focus": [17741.7], "XYStage": [ 10346.1, 77.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos31", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.49], "Focus": [17741.4], "XYStage": [ 9755.5, 85.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos32", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.475], "Focus": [17741.5], "XYStage": [ 4775.5, 293.90000000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos33", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.445], "Focus": [17741.4], "XYStage": [ 3954.9, 293.90000000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos34", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.455], "Focus": [17741.4], "XYStage": [ -1324.9, 545.6 ] }, "GridColumnIndex": 0 }, { "Label": "Pos35", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.445], "Focus": [17741.3], "XYStage": [ -2097, 576.6 ] }, "GridColumnIndex": 0 } ], "z-step_um": 10, "GridRow": 0, "BitDepth": 16, "Depth": 2, "Channels": 6, "Source": "Micro-Manager", "ChColors": [ -16711681, -256, -65281, -1, -1, -16776961 ], "MetadataVersion": 10, "KeepShutterOpenChannels": true, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536, 65536 ], "CustomIntervals_ms": [], "Height": 1200, "MicroManagerVersion": "1.4.23 20180219", "GridColumn": 0, "UUID": "67598d21-5db5-4842-ab60-f506f7b42c7c", "PixelType": "GRAY16", "Width": 1200, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\Yan\\20191218-YC113-S7S8-miseq", "Frames": 1, "Comment": "", "UserName": "Nikon", "PixelSize_um": 1.1, "ROI": [ 0, 0, 1200, 1200 ], "Date": "2019-12-18", "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC", "P-DAPI-high" ], "KeepShutterOpenSlices": true, "IJType": 1, "ComputerName": "ZadorConfocal2", "Positions": 36, "PositionIndex": 29, "CameraTimeout": 20000, "TimeFirst": false }, "640 AO-Name": "AnalogIO", "Vincent-D3-Command": "Close" }, "FrameKey-0-2-5": { "COM4-StopBits": "1", "528 AO-MaxVolts": "5.0000", "XLIGHT Excitation Wheel-State": "1", "470 Shutter-Name": "AnalogIO", "Core-Shutter": "640", "555 AO-Demo": "No", "XLIGHT Dichroic-State": "2", "XYStage-StepSize-Y": "0.1000", "ZPositionUm": 218.48000000000002, "XYStage-StepSize-X": "0.1000", "PRIME-FirmwareVersion": "20.28", "PRIME-ClearCycles": "2", "520-Name": "DA Shutter", "405 Shutter-Name": "AnalogIO", "PIEZO-StepSize": "0.0050", "PRIME-ClearMode": "Pre-Exposure", "470 AO-MinVolts": "0.0000", "405 AO-SupportsTriggering": "1", "CameraChannelIndex": 0, "XYStage-StartSpeed": "500.0000", "COM9-StopBits": "2", "405 AO-Description": "NI DAC", "COM4-DelayBetweenCharsMs": "0.0000", "445 AO-Block voltage": "false", "520 AO-TriggerInputLine": "/Dev1/PFI0", "555 AO-Description": "NI DAC", "520 AO-TriggerSampleRate": "100000", "COM4-Verbose": "1", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM4-Name": "COM4", "405 Shutter-Volts": "0.0000", "Vincent-D3-Port": "COM1", "PRIME-PP 4 MIN ADU AFFECTED": "200", "470 Shutter-MinVolts": "0.0000", "PRIME-PP 3 ENABLED": "Yes", "470 AO-Description": "NI DAC", "PRIME-Description": "PVCAM API device adapter", "520 Shutter-MaxVolts": "5.0000", "520 Shutter-Volts": "0.0000", "528 AO-Sequenceable": "1", "PRIME-Binning": "1x1", "555 Shutter-MaxVolts": "5.0000", "528 Shutter-TriggerSequenceLength": "1000", "405 Shutter-Block voltage": "false", "XLIGHT Spin motor-State": "1", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "Vincent-D3-Time to open (ms)": "35.0000", "Vincent-D3-Time to close (ms)": "35.0000", "445 AO-TriggerSampleRate": "100000", "640 Shutter-IOChannel": "Dev1/ao14", "555 AO-Sequenceable": "1", "520 AO-Name": "AnalogIO", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "470 AO-Block voltage": "false", "COM1-Verbose": "1", "COM9-AnswerTimeout": "10000.0000", "528 Shutter-Name": "AnalogIO", "PRIME-Trigger Timeout (secs)": "10", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "NextFrame": 0, "470 AO-TriggerSequenceLength": "1000", "PRIME-TransposeXY": "0", "445 AO-TriggerSequenceLength": "1000", "COM1-Name": "COM1", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "COM9-DataBits": "8", "COM1-AnswerTimeout": "500.0000", "Camera": "", "TE2000-Version": "V2.50-V2.50-V2.10", "528 Shutter-Volts": "0.0000", "XLIGHT Hub-Name": "XLIGHT Hub", "PIEZO-Description": "Ludl stage driver adapter", "PositionName": "Pos29", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSampleRate": "100000", "PRIME-FanSpeedSetpoint": "High", "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "405 AO-TriggerSampleRate": "100000", "470 Shutter-Block voltage": "false", "470-State": "0", "COM3-Parity": "None", "405 AO-Volts": "0.0000", "PRIME-TransposeMirrorY": "0", "PRIME-ChipName": "GS144BSI", "470 AO-Volts": "0.0000", "PRIME-TransposeMirrorX": "0", "445 Shutter-SupportsTriggering": "0", "470 Shutter-SupportsTriggering": "0", "XLIGHT Hub-Description": "XLIGHT hub", "PRIME-PreampOffLimit": "10000", "470 AO-Sequenceable": "1", "528 Shutter-MaxVolts": "5.0000", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "Core-XYStage": "XYStage", "470 AO-TriggerSampleRate": "100000", "PRIME-Timing-PreTriggerDelayNs": "24672000.0000", "528-DA Device": "528 Shutter", "Focus-Description": "Nikon TE2000 focus adapter", "FilterBlock-State": "5", "520 AO-Demo": "No", "COM3-DelayBetweenCharsMs": "0.0000", "470 Shutter-Volts": "0.0000", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "PRIME-PP 2 THRESHOLD": "125", "PRIME-PP 4 THRESHOLD": "75", "XYStage-Acceleration": "4.0000", "470 Shutter-Demo": "No", "PRIME-Name": "PMPCIECam00", "640-DA Device": "640 Shutter", "405 AO-Block voltage": "false", "ElapsedTime-ms": 1205651, "PRIME-Offset": "100", "445 AO-IOChannel": "Dev1/ao1", "640 AO-MaxVolts": "5.0000", "PositionIndex": 29, "PRIME-PP 1 ENABLED": "Yes", "COM1-BaudRate": "9600", "640-State": "1", "COM3-Verbose": "1", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "LudlController-TransmissionDelay": "0", "PRIME-FullWellCapacity": "62000", "PRIME-SMARTStreamingEnabled": "No", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "640 Shutter-Description": "NI DAC", "528-Description": "DA device that is used as a shutter", "COM4-Description": "Serial port driver (boost:asio)", "405 Shutter-Description": "NI DAC", "TE2000-Name": "TE2000", "528 Shutter-Description": "NI DAC", "COM9-Description": "Serial port driver (boost:asio)", "LudlController-Name": "LudlController", "445 Shutter-TriggerSequenceLength": "1000", "PRIME-SerialNumber": "A18B203008", "445 AO-Volts": "0.0000", "PRIME-X-dimension": "1200", "PRIME-ShutterCloseDelay": "0", "XLIGHT Disk slider-State": "2", "Position": "Default", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 AO-IOChannel": "Dev1/ao5", "520 AO-IOChannel": "Dev1/ao4", "528 AO-TriggerSequenceLength": "1000", "405 Shutter-MaxVolts": "5.0000", "470-DA Device": "470 Shutter", "PRIME-FTCapable": "No", "Core-ChannelGroup": "Channels", "555 AO-SupportsTriggering": "0", "PRIME-Y-dimension": "1200", "Frame": 0, "555 Shutter-Sequenceable": "1", "PRIME-Timing-ExposureTimeNs": "499998000.0000", "Core-ImageProcessor": "", "528-State": "0", "520 AO-Block voltage": "false", "XLIGHT Excitation Wheel-Label": "QUAD", "640 Shutter-Demo": "No", "COM3-StopBits": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "520 Shutter-IOChannel": "Dev1/ao11", "Width": 1200, "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-SupportsTriggering": "0", "640 AO-TriggerSampleRate": "100000", "520-Description": "DA device that is used as a shutter", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "405 Shutter-Sequenceable": "1", "405 Shutter-SupportsTriggering": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-TriggerSampleRate": "100000", "640 Shutter-MaxVolts": "5.0000", "555 AO-MaxVolts": "5.0000", "520 AO-TriggerSequenceLength": "1000", "COM3-BaudRate": "9600", "COM9-BaudRate": "9600", "Vincent-D3-Channel #": "Ch. #1", "PRIME-PMode": "Normal", "OpticalPath-Label": "Left(100)", "BitDepth": 16, "555 AO-TriggerSequenceLength": "1000", "445 Shutter-Description": "NI DAC", "405 AO-Sequenceable": "1", "PRIME-AcquisitionMethod": "Callbacks", "555 AO-Name": "AnalogIO", "PRIME-ShutterMode": "Pre-Exposure", "COM3-Handshaking": "Off", "445 AO-SupportsTriggering": "0", "ROI": "0-0-1200-1200", "COM9-Verbose": "1", "PRIME-Color": "OFF", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "PRIME-ShutterOpenDelay": "0", "COM1-Parity": "None", "Slice": 5, "SlicePosition": 0, "COM9-DelayBetweenCharsMs": "0.0000", "555-Name": "DA Shutter", "PIEZO-LudlSingleAxisName": "B", "XLIGHT Emission Wheel-Label": "679/29 (A)", "640 Shutter-Name": "AnalogIO", "640 AO-Sequenceable": "1", "520 Shutter-Demo": "No", "528 Shutter-Demo": "No", "470-Name": "DA Shutter", "520-DA Device": "520 Shutter", "XYStage-Description": "Ludl XY stage driver adapter", "445 Shutter-MinVolts": "0.0000", "528 AO-TriggerSampleRate": "100000", "405 AO-MinVolts": "0.0000", "405 Shutter-TriggerSequenceLength": "1000", "Height": 1200, "555 AO-Block voltage": "false", "640 Shutter-Volts": "5.0000", "PixelType": "GRAY16", "445 Shutter-Block voltage": "false", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "520 Shutter-TriggerSequenceLength": "1000", "COM9-Name": "COM9", "Core-SLM": "", "555 AO-Volts": "0.0000", "555 Shutter-Block voltage": "false", "XYStage-Speed": "1800.0000", "520 AO-MaxVolts": "5.0000", "528 Shutter-SupportsTriggering": "0", "COM1-Description": "Serial port driver (boost:asio)", "PRIME-MetadataEnabled": "No", "520 Shutter-MinVolts": "0.0000", "470 Shutter-Sequenceable": "1", "PRIME-PreampDelay": "0", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-MinVolts": "0.0000", "ChannelIndex": 2, "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Core-AutoShutter": "1", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "Exposure-ms": 320, "PRIME-PP 2 ENABLED": "Yes", "640 Shutter-TriggerSampleRate": "100000", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "470 Shutter-IOChannel": "Dev1/ao10", "405 AO-Demo": "No", "PixelSizeUm": 1.1, "470 AO-Demo": "No", "470 Shutter-MaxVolts": "5.0000", "Vincent-D3-Name": "Vincent-D3", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FileName": "img_000000000_Il-A_005.tif", "LudlController-Reset": "Operate", "PRIME-Exposure": "320.00", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "445-Name": "DA Shutter", "555 Shutter-Name": "AnalogIO", "520 Shutter-TriggerSampleRate": "100000", "445 Shutter-IOChannel": "Dev1/ao9", "PRIME-TransposeCorrection": "0", "XYStage-StepSize": "0.1000", "445-Description": "DA device that is used as a shutter", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 AO-IOChannel": "Dev1/ao7", "520 Shutter-Name": "AnalogIO", "COM3-DataBits": "8", "640 AO-Volts": "4.5000", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "555 Shutter-IOChannel": "Dev1/ao13", "WaitInterval": null, "520 Shutter-Sequenceable": "1", "YPositionUm": -180, "445-State": "0", "445-DA Device": "445 Shutter", "PRIME-CircularBufferAutoSize": "ON", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "555 AO-TriggerSampleRate": "100000", "445 Shutter-Volts": "0.0000", "XLIGHT Hub-Port": "COM3", "Core-AutoFocus": "", "COM4-Handshaking": "Off", "528 Shutter-MinVolts": "0.0000", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "445 Shutter-Name": "AnalogIO", "528 Shutter-Sequenceable": "1", "XYStage-TransposeMirrorX": "0", "XYStage-TransposeMirrorY": "0", "PRIME-ReadoutRate": "100MHz 16bit", "XYStage-Name": "Stage", "640 AO-SupportsTriggering": "0", "640-Name": "DA Shutter", "640 Shutter-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "640 AO-Block voltage": "false", "445 Shutter-Sequenceable": "1", "470 AO-MaxVolts": "5.0000", "COM4-BaudRate": "9600", "470-Description": "DA device that is used as a shutter", "520 Shutter-Description": "NI DAC", "PRIME-CCDTemperature": "-14.8500", "Time": "2019-12-18 23:11:05 -0500", "640 AO-TriggerSequenceLength": "1000", "470 AO-Name": "AnalogIO", "XLIGHT Disk slider-Label": "Disk Pos 40um", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "Binning": "1x1", "TE2000-ModelType": "1", "COM3-AnswerTimeout": "500.0000", "555 Shutter-SupportsTriggering": "0", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "TE2000-Port": "COM4", "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Description": "NI DAC", "COM4-AnswerTimeout": "500.0000", "COM1-DelayBetweenCharsMs": "0.0000", "FilterBlock-Label": "Confocal", "445 AO-Name": "AnalogIO", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-Timing-ReadoutTimeNs": "24672000.0000", "528 AO-Demo": "No", "520 AO-Sequenceable": "1", "555-Description": "DA device that is used as a shutter", "405-DA Device": "405 Shutter", "405 AO-TriggerSequenceLength": "1000", "640 AO-MinVolts": "0.0000", "640 Shutter-Block voltage": "false", "FrameIndex": 0, "PRIME-Port": "CMOS", "555 AO-MinVolts": "0.0000", "COM3-Description": "Serial port driver (boost:asio)", "528 AO-Description": "NI DAC", "555 AO-IOChannel": "Dev1/ao6", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "555 AO-TriggerInputLine": "/Dev1/PFI0", "PRIME-ActualInterval-ms": "390.5200", "LudlController-Port": "COM9", "445 Shutter-Demo": "No", "PRIME-Trigger-Expose Out-Mux": "1", "SliceIndex": 5, "PRIME-CCDTemperatureSetPoint": "-15.0000", "405 Shutter-MinVolts": "0.0000", "PRIME-ExposeOutMode": "First Row", "AxisPositions": null, "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Volts": "0.0000", "528-Name": "DA Shutter", "555-State": "0", "XLIGHT Dichroic-Label": "Di03-R635-t1", "445 AO-Sequenceable": "1", "405-Name": "DA Shutter", "405 AO-IOChannel": "Dev1/ao0", "Core-Camera": "PRIME", "Focus-Name": "Focus", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "405 Shutter-IOChannel": "Dev1/ao8", "640 AO-Demo": "No", "640 Shutter-SupportsTriggering": "0", "445 AO-Demo": "No", "555-DA Device": "555 Shutter", "470 Shutter-Description": "NI DAC", "528 AO-Volts": "0.0000", "COM1-DataBits": "8", "Vincent-D3-Address": "x", "520 AO-SupportsTriggering": "0", "405-Description": "DA device that is used as a shutter", "640 Shutter-MinVolts": "0.0000", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "405 AO-Name": "AnalogIO", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "640 AO-TriggerInputLine": "/Dev1/PFI0", "405 AO-TriggerInputLine": "/Dev1/PFI0", "640-Description": "DA device that is used as a shutter", "445 Shutter-TriggerSampleRate": "100000", "470 Shutter-TriggerSequenceLength": "1000", "COM1-StopBits": "1", "520 Shutter-SupportsTriggering": "0", "405-State": "0", "Core-Initialize": "1", "XLIGHT Emission Wheel-State": "3", "520 AO-Description": "NI DAC", "528 AO-TriggerInputLine": "/Dev1/PFI0", "445 Shutter-MaxVolts": "5.0000", "COM9-Handshaking": "Off", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "XLIGHT Spin motor-Label": "On (spinning)", "PRIME-PP 5 ENABLED": "No", "UUID": "395bdb10-13ba-408a-8e29-8e2c57a3f287", "PRIME-TriggerMode": "Internal Trigger", "COM1-Handshaking": "Off", "PRIME-Timing-PostTriggerDelayNs": "49344000.0000", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "Focus-Position": "17741.5000", "528 Shutter-Block voltage": "false", "Channel": "Il-A", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "555 Shutter-Demo": "No", "405 Shutter-Demo": "No", "528 AO-Name": "AnalogIO", "520-State": "0", "Core-Galvo": "", "405 Shutter-TriggerSampleRate": "100000", "Focus-FocusStepSize_(nm)": "100", "PRIME-PixelType": "16bit", "COM4-DataBits": "8", "470 AO-IOChannel": "Dev1/ao2", "520 AO-MinVolts": "0.0000", "COM9-Parity": "None", "PRIME-CircularBufferEnabled": "ON", "PRIME-PP 6 Reset": "No", "640 AO-Description": "NI DAC", "Source": "PRIME", "445 AO-MinVolts": "0.0000", "PIEZO-Name": "Stage", "445 AO-Description": "NI DAC", "PRIME-CircularBufferFrameCount": "46", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "520 AO-Volts": "0.0000", "528 Shutter-TriggerSampleRate": "100000", "XPositionUm": 15712.1, "OpticalPath-State": "4", "PRIME-PP 3 THRESHOLD": "80", "PRIME-PVCAM Version": "3.7.1", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Gain": "1-HDR", "640 Shutter-TriggerSequenceLength": "1000", "528 AO-SupportsTriggering": "0", "445 AO-TriggerInputLine": "/Dev1/PFI0", "OpticalPath-Name": "OpticalPath", "PRIME-PP 4 ENABLED": "Yes", "PRIME-PVCAM Adapter Version": "1.3.29", "COM3-Name": "COM3", "405 AO-MaxVolts": "5.0000", "520 Shutter-Block voltage": "false", "PRIME-Timing-ClearingTimeNs": "49344000.0000", "Core-Focus": "PIEZO", "528 AO-Block voltage": "false", "TE2000-Description": "Nikon TE2000 hub", "528 AO-MinVolts": "0.0000", "COM4-Parity": "None", "Summary": { "Slices": 11, "ChContrastMin": [ 0, 0, 0, 0, 0, 0 ], "PixelAspect": 1, "SlicesFirst": true, "Interval_ms": 0, "Time": "2019-12-18 23:10:50 -0500", "Prefix": "seq_5", "InitialPositionList": [ { "Label": "Pos0", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.21], "Focus": [17741.5], "XYStage": [ 44542.200000000004, 31636.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos1", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.22], "Focus": [17741.4], "XYStage": [ 43801.100000000006, 31637.9 ] }, "GridColumnIndex": 0 }, { "Label": "Pos2", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.5], "XYStage": [ 38561.3, 32040.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos3", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.185], "Focus": [17741.3], "XYStage": [ 37698.4, 32041.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos4", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.16], "Focus": [17741.5], "XYStage": [ 32375.4, 31758.9 ] }, "GridColumnIndex": 0 }, { "Label": "Pos5", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.17000000000002], "Focus": [17741.3], "XYStage": [ 31770.7, 31758.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos6", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.4], "XYStage": [ 26536.800000000003, 31068.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos7", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.205], "Focus": [17741.4], "XYStage": [ 25420.2, 31068.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos8", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.85], "Focus": [17741.3], "XYStage": [ 21059, 31508 ] }, "GridColumnIndex": 0 }, { "Label": "Pos9", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.82], "Focus": [17741.3], "XYStage": [ 19731.9, 31506.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos10", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.79], "Focus": [17741.4], "XYStage": [ 14528.5, 32007.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos11", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.83], "Focus": [17741.2], "XYStage": [ 13830.300000000001, 32009.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos12", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.79], "Focus": [17741.3], "XYStage": [ 8775.300000000001, 32017.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos13", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.815], "Focus": [17741.3], "XYStage": [ 8109.200000000001, 32008.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos14", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.805], "Focus": [17741.3], "XYStage": [ 3483.3, 31908.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos15", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.82], "Focus": [17741.2], "XYStage": [ 2686.8, 31903.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos16", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [211.48000000000002], "Focus": [17741.1], "XYStage": [ -1978.9, 31973.600000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos17", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [211.51500000000001], "Focus": [17741.1], "XYStage": [ -2957.8, 31982.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos18", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.195], "Focus": [17741.5], "XYStage": [ 47747.4, -576.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos19", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.23000000000002], "Focus": [17741.6], "XYStage": [ 47053.9, -576.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos20", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.205], "Focus": [17741.4], "XYStage": [ 41871.200000000004, -479.8 ] }, "GridColumnIndex": 0 }, { "Label": "Pos21", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.4], "XYStage": [ 40882.600000000006, -392 ] }, "GridColumnIndex": 0 }, { "Label": "Pos22", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.24], "Focus": [17741.4], "XYStage": [ 35905.6, -135.3 ] }, "GridColumnIndex": 0 }, { "Label": "Pos23", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.195], "Focus": [17741.5], "XYStage": [ 34990.9, -132.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos24", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.46], "Focus": [17741.4], "XYStage": [ 29678.100000000002, -958.1 ] }, "GridColumnIndex": 0 }, { "Label": "Pos25", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.44], "Focus": [17741.5], "XYStage": [ 28983.600000000002, -939.4000000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos26", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.475], "Focus": [17741.5], "XYStage": [ 23019.7, -122 ] }, "GridColumnIndex": 0 }, { "Label": "Pos27", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.44], "Focus": [17741.7], "XYStage": [ 22428, -116.80000000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos28", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.46], "Focus": [17741.7], "XYStage": [ 16364.900000000001, -181.10000000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos29", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.48000000000002], "Focus": [17741.5], "XYStage": [ 15712.1, -180 ] }, "GridColumnIndex": 0 }, { "Label": "Pos30", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.45000000000002], "Focus": [17741.7], "XYStage": [ 10346.1, 77.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos31", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.49], "Focus": [17741.4], "XYStage": [ 9755.5, 85.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos32", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.475], "Focus": [17741.5], "XYStage": [ 4775.5, 293.90000000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos33", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.445], "Focus": [17741.4], "XYStage": [ 3954.9, 293.90000000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos34", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.455], "Focus": [17741.4], "XYStage": [ -1324.9, 545.6 ] }, "GridColumnIndex": 0 }, { "Label": "Pos35", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.445], "Focus": [17741.3], "XYStage": [ -2097, 576.6 ] }, "GridColumnIndex": 0 } ], "z-step_um": 10, "GridRow": 0, "BitDepth": 16, "Depth": 2, "Channels": 6, "Source": "Micro-Manager", "ChColors": [ -16711681, -256, -65281, -1, -1, -16776961 ], "MetadataVersion": 10, "KeepShutterOpenChannels": true, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536, 65536 ], "CustomIntervals_ms": [], "Height": 1200, "MicroManagerVersion": "1.4.23 20180219", "GridColumn": 0, "UUID": "67598d21-5db5-4842-ab60-f506f7b42c7c", "PixelType": "GRAY16", "Width": 1200, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\Yan\\20191218-YC113-S7S8-miseq", "Frames": 1, "Comment": "", "UserName": "Nikon", "PixelSize_um": 1.1, "ROI": [ 0, 0, 1200, 1200 ], "Date": "2019-12-18", "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC", "P-DAPI-high" ], "KeepShutterOpenSlices": true, "IJType": 1, "ComputerName": "ZadorConfocal2", "Positions": 36, "PositionIndex": 29, "CameraTimeout": 20000, "TimeFirst": false }, "640 AO-Name": "AnalogIO", "Vincent-D3-Command": "Close" }, "FrameKey-0-2-6": { "COM4-StopBits": "1", "528 AO-MaxVolts": "5.0000", "XLIGHT Excitation Wheel-State": "1", "470 Shutter-Name": "AnalogIO", "Core-Shutter": "640", "555 AO-Demo": "No", "XLIGHT Dichroic-State": "2", "XYStage-StepSize-Y": "0.1000", "ZPositionUm": 228.48000000000002, "XYStage-StepSize-X": "0.1000", "PRIME-FirmwareVersion": "20.28", "PRIME-ClearCycles": "2", "520-Name": "DA Shutter", "405 Shutter-Name": "AnalogIO", "PIEZO-StepSize": "0.0050", "PRIME-ClearMode": "Pre-Exposure", "470 AO-MinVolts": "0.0000", "405 AO-SupportsTriggering": "1", "CameraChannelIndex": 0, "XYStage-StartSpeed": "500.0000", "COM9-StopBits": "2", "405 AO-Description": "NI DAC", "COM4-DelayBetweenCharsMs": "0.0000", "445 AO-Block voltage": "false", "520 AO-TriggerInputLine": "/Dev1/PFI0", "555 AO-Description": "NI DAC", "520 AO-TriggerSampleRate": "100000", "COM4-Verbose": "1", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM4-Name": "COM4", "405 Shutter-Volts": "0.0000", "Vincent-D3-Port": "COM1", "PRIME-PP 4 MIN ADU AFFECTED": "200", "470 Shutter-MinVolts": "0.0000", "PRIME-PP 3 ENABLED": "Yes", "470 AO-Description": "NI DAC", "PRIME-Description": "PVCAM API device adapter", "520 Shutter-MaxVolts": "5.0000", "520 Shutter-Volts": "0.0000", "528 AO-Sequenceable": "1", "PRIME-Binning": "1x1", "555 Shutter-MaxVolts": "5.0000", "528 Shutter-TriggerSequenceLength": "1000", "405 Shutter-Block voltage": "false", "XLIGHT Spin motor-State": "1", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "Vincent-D3-Time to open (ms)": "35.0000", "Vincent-D3-Time to close (ms)": "35.0000", "445 AO-TriggerSampleRate": "100000", "640 Shutter-IOChannel": "Dev1/ao14", "555 AO-Sequenceable": "1", "520 AO-Name": "AnalogIO", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "470 AO-Block voltage": "false", "COM1-Verbose": "1", "COM9-AnswerTimeout": "10000.0000", "528 Shutter-Name": "AnalogIO", "PRIME-Trigger Timeout (secs)": "10", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "NextFrame": 0, "470 AO-TriggerSequenceLength": "1000", "PRIME-TransposeXY": "0", "445 AO-TriggerSequenceLength": "1000", "COM1-Name": "COM1", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "COM9-DataBits": "8", "COM1-AnswerTimeout": "500.0000", "Camera": "", "TE2000-Version": "V2.50-V2.50-V2.10", "528 Shutter-Volts": "0.0000", "XLIGHT Hub-Name": "XLIGHT Hub", "PIEZO-Description": "Ludl stage driver adapter", "PositionName": "Pos29", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSampleRate": "100000", "PRIME-FanSpeedSetpoint": "High", "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "405 AO-TriggerSampleRate": "100000", "470 Shutter-Block voltage": "false", "470-State": "0", "COM3-Parity": "None", "405 AO-Volts": "0.0000", "PRIME-TransposeMirrorY": "0", "PRIME-ChipName": "GS144BSI", "470 AO-Volts": "0.0000", "PRIME-TransposeMirrorX": "0", "445 Shutter-SupportsTriggering": "0", "470 Shutter-SupportsTriggering": "0", "XLIGHT Hub-Description": "XLIGHT hub", "PRIME-PreampOffLimit": "10000", "470 AO-Sequenceable": "1", "528 Shutter-MaxVolts": "5.0000", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "Core-XYStage": "XYStage", "470 AO-TriggerSampleRate": "100000", "PRIME-Timing-PreTriggerDelayNs": "24672000.0000", "528-DA Device": "528 Shutter", "Focus-Description": "Nikon TE2000 focus adapter", "FilterBlock-State": "5", "520 AO-Demo": "No", "COM3-DelayBetweenCharsMs": "0.0000", "470 Shutter-Volts": "0.0000", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "PRIME-PP 2 THRESHOLD": "125", "PRIME-PP 4 THRESHOLD": "75", "XYStage-Acceleration": "4.0000", "470 Shutter-Demo": "No", "PRIME-Name": "PMPCIECam00", "640-DA Device": "640 Shutter", "405 AO-Block voltage": "false", "ElapsedTime-ms": 1206205, "PRIME-Offset": "100", "445 AO-IOChannel": "Dev1/ao1", "640 AO-MaxVolts": "5.0000", "PositionIndex": 29, "PRIME-PP 1 ENABLED": "Yes", "COM1-BaudRate": "9600", "640-State": "1", "COM3-Verbose": "1", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "LudlController-TransmissionDelay": "0", "PRIME-FullWellCapacity": "62000", "PRIME-SMARTStreamingEnabled": "No", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "640 Shutter-Description": "NI DAC", "528-Description": "DA device that is used as a shutter", "COM4-Description": "Serial port driver (boost:asio)", "405 Shutter-Description": "NI DAC", "TE2000-Name": "TE2000", "528 Shutter-Description": "NI DAC", "COM9-Description": "Serial port driver (boost:asio)", "LudlController-Name": "LudlController", "445 Shutter-TriggerSequenceLength": "1000", "PRIME-SerialNumber": "A18B203008", "445 AO-Volts": "0.0000", "PRIME-X-dimension": "1200", "PRIME-ShutterCloseDelay": "0", "XLIGHT Disk slider-State": "2", "Position": "Default", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 AO-IOChannel": "Dev1/ao5", "520 AO-IOChannel": "Dev1/ao4", "528 AO-TriggerSequenceLength": "1000", "405 Shutter-MaxVolts": "5.0000", "470-DA Device": "470 Shutter", "PRIME-FTCapable": "No", "Core-ChannelGroup": "Channels", "555 AO-SupportsTriggering": "0", "PRIME-Y-dimension": "1200", "Frame": 0, "555 Shutter-Sequenceable": "1", "PRIME-Timing-ExposureTimeNs": "499998000.0000", "Core-ImageProcessor": "", "528-State": "0", "520 AO-Block voltage": "false", "XLIGHT Excitation Wheel-Label": "QUAD", "640 Shutter-Demo": "No", "COM3-StopBits": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "520 Shutter-IOChannel": "Dev1/ao11", "Width": 1200, "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-SupportsTriggering": "0", "640 AO-TriggerSampleRate": "100000", "520-Description": "DA device that is used as a shutter", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "405 Shutter-Sequenceable": "1", "405 Shutter-SupportsTriggering": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-TriggerSampleRate": "100000", "640 Shutter-MaxVolts": "5.0000", "555 AO-MaxVolts": "5.0000", "520 AO-TriggerSequenceLength": "1000", "COM3-BaudRate": "9600", "COM9-BaudRate": "9600", "Vincent-D3-Channel #": "Ch. #1", "PRIME-PMode": "Normal", "OpticalPath-Label": "Left(100)", "BitDepth": 16, "555 AO-TriggerSequenceLength": "1000", "445 Shutter-Description": "NI DAC", "405 AO-Sequenceable": "1", "PRIME-AcquisitionMethod": "Callbacks", "555 AO-Name": "AnalogIO", "PRIME-ShutterMode": "Pre-Exposure", "COM3-Handshaking": "Off", "445 AO-SupportsTriggering": "0", "ROI": "0-0-1200-1200", "COM9-Verbose": "1", "PRIME-Color": "OFF", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "PRIME-ShutterOpenDelay": "0", "COM1-Parity": "None", "Slice": 6, "SlicePosition": 10, "COM9-DelayBetweenCharsMs": "0.0000", "555-Name": "DA Shutter", "PIEZO-LudlSingleAxisName": "B", "XLIGHT Emission Wheel-Label": "679/29 (A)", "640 Shutter-Name": "AnalogIO", "640 AO-Sequenceable": "1", "520 Shutter-Demo": "No", "528 Shutter-Demo": "No", "470-Name": "DA Shutter", "520-DA Device": "520 Shutter", "XYStage-Description": "Ludl XY stage driver adapter", "445 Shutter-MinVolts": "0.0000", "528 AO-TriggerSampleRate": "100000", "405 AO-MinVolts": "0.0000", "405 Shutter-TriggerSequenceLength": "1000", "Height": 1200, "555 AO-Block voltage": "false", "640 Shutter-Volts": "5.0000", "PixelType": "GRAY16", "445 Shutter-Block voltage": "false", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "520 Shutter-TriggerSequenceLength": "1000", "COM9-Name": "COM9", "Core-SLM": "", "555 AO-Volts": "0.0000", "555 Shutter-Block voltage": "false", "XYStage-Speed": "1800.0000", "520 AO-MaxVolts": "5.0000", "528 Shutter-SupportsTriggering": "0", "COM1-Description": "Serial port driver (boost:asio)", "PRIME-MetadataEnabled": "No", "520 Shutter-MinVolts": "0.0000", "470 Shutter-Sequenceable": "1", "PRIME-PreampDelay": "0", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-MinVolts": "0.0000", "ChannelIndex": 2, "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Core-AutoShutter": "1", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "Exposure-ms": 320, "PRIME-PP 2 ENABLED": "Yes", "640 Shutter-TriggerSampleRate": "100000", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "470 Shutter-IOChannel": "Dev1/ao10", "405 AO-Demo": "No", "PixelSizeUm": 1.1, "470 AO-Demo": "No", "470 Shutter-MaxVolts": "5.0000", "Vincent-D3-Name": "Vincent-D3", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FileName": "img_000000000_Il-A_006.tif", "LudlController-Reset": "Operate", "PRIME-Exposure": "320.00", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "445-Name": "DA Shutter", "555 Shutter-Name": "AnalogIO", "520 Shutter-TriggerSampleRate": "100000", "445 Shutter-IOChannel": "Dev1/ao9", "PRIME-TransposeCorrection": "0", "XYStage-StepSize": "0.1000", "445-Description": "DA device that is used as a shutter", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 AO-IOChannel": "Dev1/ao7", "520 Shutter-Name": "AnalogIO", "COM3-DataBits": "8", "640 AO-Volts": "4.5000", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "555 Shutter-IOChannel": "Dev1/ao13", "WaitInterval": null, "520 Shutter-Sequenceable": "1", "YPositionUm": -180, "445-State": "0", "445-DA Device": "445 Shutter", "PRIME-CircularBufferAutoSize": "ON", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "555 AO-TriggerSampleRate": "100000", "445 Shutter-Volts": "0.0000", "XLIGHT Hub-Port": "COM3", "Core-AutoFocus": "", "COM4-Handshaking": "Off", "528 Shutter-MinVolts": "0.0000", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "445 Shutter-Name": "AnalogIO", "528 Shutter-Sequenceable": "1", "XYStage-TransposeMirrorX": "0", "XYStage-TransposeMirrorY": "0", "PRIME-ReadoutRate": "100MHz 16bit", "XYStage-Name": "Stage", "640 AO-SupportsTriggering": "0", "640-Name": "DA Shutter", "640 Shutter-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "640 AO-Block voltage": "false", "445 Shutter-Sequenceable": "1", "470 AO-MaxVolts": "5.0000", "COM4-BaudRate": "9600", "470-Description": "DA device that is used as a shutter", "520 Shutter-Description": "NI DAC", "PRIME-CCDTemperature": "-14.8500", "Time": "2019-12-18 23:11:06 -0500", "640 AO-TriggerSequenceLength": "1000", "470 AO-Name": "AnalogIO", "XLIGHT Disk slider-Label": "Disk Pos 40um", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "Binning": "1x1", "TE2000-ModelType": "1", "COM3-AnswerTimeout": "500.0000", "555 Shutter-SupportsTriggering": "0", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "TE2000-Port": "COM4", "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Description": "NI DAC", "COM4-AnswerTimeout": "500.0000", "COM1-DelayBetweenCharsMs": "0.0000", "FilterBlock-Label": "Confocal", "445 AO-Name": "AnalogIO", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-Timing-ReadoutTimeNs": "24672000.0000", "528 AO-Demo": "No", "520 AO-Sequenceable": "1", "555-Description": "DA device that is used as a shutter", "405-DA Device": "405 Shutter", "405 AO-TriggerSequenceLength": "1000", "640 AO-MinVolts": "0.0000", "640 Shutter-Block voltage": "false", "FrameIndex": 0, "PRIME-Port": "CMOS", "555 AO-MinVolts": "0.0000", "COM3-Description": "Serial port driver (boost:asio)", "528 AO-Description": "NI DAC", "555 AO-IOChannel": "Dev1/ao6", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "555 AO-TriggerInputLine": "/Dev1/PFI0", "PRIME-ActualInterval-ms": "390.5200", "LudlController-Port": "COM9", "445 Shutter-Demo": "No", "PRIME-Trigger-Expose Out-Mux": "1", "SliceIndex": 6, "PRIME-CCDTemperatureSetPoint": "-15.0000", "405 Shutter-MinVolts": "0.0000", "PRIME-ExposeOutMode": "First Row", "AxisPositions": null, "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Volts": "0.0000", "528-Name": "DA Shutter", "555-State": "0", "XLIGHT Dichroic-Label": "Di03-R635-t1", "445 AO-Sequenceable": "1", "405-Name": "DA Shutter", "405 AO-IOChannel": "Dev1/ao0", "Core-Camera": "PRIME", "Focus-Name": "Focus", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "405 Shutter-IOChannel": "Dev1/ao8", "640 AO-Demo": "No", "640 Shutter-SupportsTriggering": "0", "445 AO-Demo": "No", "555-DA Device": "555 Shutter", "470 Shutter-Description": "NI DAC", "528 AO-Volts": "0.0000", "COM1-DataBits": "8", "Vincent-D3-Address": "x", "520 AO-SupportsTriggering": "0", "405-Description": "DA device that is used as a shutter", "640 Shutter-MinVolts": "0.0000", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "405 AO-Name": "AnalogIO", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "640 AO-TriggerInputLine": "/Dev1/PFI0", "405 AO-TriggerInputLine": "/Dev1/PFI0", "640-Description": "DA device that is used as a shutter", "445 Shutter-TriggerSampleRate": "100000", "470 Shutter-TriggerSequenceLength": "1000", "COM1-StopBits": "1", "520 Shutter-SupportsTriggering": "0", "405-State": "0", "Core-Initialize": "1", "XLIGHT Emission Wheel-State": "3", "520 AO-Description": "NI DAC", "528 AO-TriggerInputLine": "/Dev1/PFI0", "445 Shutter-MaxVolts": "5.0000", "COM9-Handshaking": "Off", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "XLIGHT Spin motor-Label": "On (spinning)", "PRIME-PP 5 ENABLED": "No", "UUID": "865d8294-d2cd-4517-aee1-c87be9323484", "PRIME-TriggerMode": "Internal Trigger", "COM1-Handshaking": "Off", "PRIME-Timing-PostTriggerDelayNs": "49344000.0000", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "Focus-Position": "17741.5000", "528 Shutter-Block voltage": "false", "Channel": "Il-A", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "555 Shutter-Demo": "No", "405 Shutter-Demo": "No", "528 AO-Name": "AnalogIO", "520-State": "0", "Core-Galvo": "", "405 Shutter-TriggerSampleRate": "100000", "Focus-FocusStepSize_(nm)": "100", "PRIME-PixelType": "16bit", "COM4-DataBits": "8", "470 AO-IOChannel": "Dev1/ao2", "520 AO-MinVolts": "0.0000", "COM9-Parity": "None", "PRIME-CircularBufferEnabled": "ON", "PRIME-PP 6 Reset": "No", "640 AO-Description": "NI DAC", "Source": "PRIME", "445 AO-MinVolts": "0.0000", "PIEZO-Name": "Stage", "445 AO-Description": "NI DAC", "PRIME-CircularBufferFrameCount": "46", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "520 AO-Volts": "0.0000", "528 Shutter-TriggerSampleRate": "100000", "XPositionUm": 15712.1, "OpticalPath-State": "4", "PRIME-PP 3 THRESHOLD": "80", "PRIME-PVCAM Version": "3.7.1", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Gain": "1-HDR", "640 Shutter-TriggerSequenceLength": "1000", "528 AO-SupportsTriggering": "0", "445 AO-TriggerInputLine": "/Dev1/PFI0", "OpticalPath-Name": "OpticalPath", "PRIME-PP 4 ENABLED": "Yes", "PRIME-PVCAM Adapter Version": "1.3.29", "COM3-Name": "COM3", "405 AO-MaxVolts": "5.0000", "520 Shutter-Block voltage": "false", "PRIME-Timing-ClearingTimeNs": "49344000.0000", "Core-Focus": "PIEZO", "528 AO-Block voltage": "false", "TE2000-Description": "Nikon TE2000 hub", "528 AO-MinVolts": "0.0000", "COM4-Parity": "None", "Summary": { "Slices": 11, "ChContrastMin": [ 0, 0, 0, 0, 0, 0 ], "PixelAspect": 1, "SlicesFirst": true, "Interval_ms": 0, "Time": "2019-12-18 23:10:50 -0500", "Prefix": "seq_5", "InitialPositionList": [ { "Label": "Pos0", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.21], "Focus": [17741.5], "XYStage": [ 44542.200000000004, 31636.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos1", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.22], "Focus": [17741.4], "XYStage": [ 43801.100000000006, 31637.9 ] }, "GridColumnIndex": 0 }, { "Label": "Pos2", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.5], "XYStage": [ 38561.3, 32040.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos3", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.185], "Focus": [17741.3], "XYStage": [ 37698.4, 32041.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos4", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.16], "Focus": [17741.5], "XYStage": [ 32375.4, 31758.9 ] }, "GridColumnIndex": 0 }, { "Label": "Pos5", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.17000000000002], "Focus": [17741.3], "XYStage": [ 31770.7, 31758.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos6", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.4], "XYStage": [ 26536.800000000003, 31068.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos7", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.205], "Focus": [17741.4], "XYStage": [ 25420.2, 31068.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos8", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.85], "Focus": [17741.3], "XYStage": [ 21059, 31508 ] }, "GridColumnIndex": 0 }, { "Label": "Pos9", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.82], "Focus": [17741.3], "XYStage": [ 19731.9, 31506.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos10", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.79], "Focus": [17741.4], "XYStage": [ 14528.5, 32007.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos11", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.83], "Focus": [17741.2], "XYStage": [ 13830.300000000001, 32009.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos12", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.79], "Focus": [17741.3], "XYStage": [ 8775.300000000001, 32017.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos13", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.815], "Focus": [17741.3], "XYStage": [ 8109.200000000001, 32008.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos14", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.805], "Focus": [17741.3], "XYStage": [ 3483.3, 31908.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos15", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.82], "Focus": [17741.2], "XYStage": [ 2686.8, 31903.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos16", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [211.48000000000002], "Focus": [17741.1], "XYStage": [ -1978.9, 31973.600000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos17", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [211.51500000000001], "Focus": [17741.1], "XYStage": [ -2957.8, 31982.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos18", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.195], "Focus": [17741.5], "XYStage": [ 47747.4, -576.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos19", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.23000000000002], "Focus": [17741.6], "XYStage": [ 47053.9, -576.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos20", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.205], "Focus": [17741.4], "XYStage": [ 41871.200000000004, -479.8 ] }, "GridColumnIndex": 0 }, { "Label": "Pos21", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.4], "XYStage": [ 40882.600000000006, -392 ] }, "GridColumnIndex": 0 }, { "Label": "Pos22", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.24], "Focus": [17741.4], "XYStage": [ 35905.6, -135.3 ] }, "GridColumnIndex": 0 }, { "Label": "Pos23", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.195], "Focus": [17741.5], "XYStage": [ 34990.9, -132.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos24", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.46], "Focus": [17741.4], "XYStage": [ 29678.100000000002, -958.1 ] }, "GridColumnIndex": 0 }, { "Label": "Pos25", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.44], "Focus": [17741.5], "XYStage": [ 28983.600000000002, -939.4000000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos26", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.475], "Focus": [17741.5], "XYStage": [ 23019.7, -122 ] }, "GridColumnIndex": 0 }, { "Label": "Pos27", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.44], "Focus": [17741.7], "XYStage": [ 22428, -116.80000000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos28", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.46], "Focus": [17741.7], "XYStage": [ 16364.900000000001, -181.10000000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos29", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.48000000000002], "Focus": [17741.5], "XYStage": [ 15712.1, -180 ] }, "GridColumnIndex": 0 }, { "Label": "Pos30", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.45000000000002], "Focus": [17741.7], "XYStage": [ 10346.1, 77.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos31", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.49], "Focus": [17741.4], "XYStage": [ 9755.5, 85.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos32", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.475], "Focus": [17741.5], "XYStage": [ 4775.5, 293.90000000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos33", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.445], "Focus": [17741.4], "XYStage": [ 3954.9, 293.90000000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos34", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.455], "Focus": [17741.4], "XYStage": [ -1324.9, 545.6 ] }, "GridColumnIndex": 0 }, { "Label": "Pos35", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.445], "Focus": [17741.3], "XYStage": [ -2097, 576.6 ] }, "GridColumnIndex": 0 } ], "z-step_um": 10, "GridRow": 0, "BitDepth": 16, "Depth": 2, "Channels": 6, "Source": "Micro-Manager", "ChColors": [ -16711681, -256, -65281, -1, -1, -16776961 ], "MetadataVersion": 10, "KeepShutterOpenChannels": true, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536, 65536 ], "CustomIntervals_ms": [], "Height": 1200, "MicroManagerVersion": "1.4.23 20180219", "GridColumn": 0, "UUID": "67598d21-5db5-4842-ab60-f506f7b42c7c", "PixelType": "GRAY16", "Width": 1200, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\Yan\\20191218-YC113-S7S8-miseq", "Frames": 1, "Comment": "", "UserName": "Nikon", "PixelSize_um": 1.1, "ROI": [ 0, 0, 1200, 1200 ], "Date": "2019-12-18", "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC", "P-DAPI-high" ], "KeepShutterOpenSlices": true, "IJType": 1, "ComputerName": "ZadorConfocal2", "Positions": 36, "PositionIndex": 29, "CameraTimeout": 20000, "TimeFirst": false }, "640 AO-Name": "AnalogIO", "Vincent-D3-Command": "Close" }, "FrameKey-0-2-7": { "COM4-StopBits": "1", "528 AO-MaxVolts": "5.0000", "XLIGHT Excitation Wheel-State": "1", "470 Shutter-Name": "AnalogIO", "Core-Shutter": "640", "555 AO-Demo": "No", "XLIGHT Dichroic-State": "2", "XYStage-StepSize-Y": "0.1000", "ZPositionUm": 238.48000000000002, "XYStage-StepSize-X": "0.1000", "PRIME-FirmwareVersion": "20.28", "PRIME-ClearCycles": "2", "520-Name": "DA Shutter", "405 Shutter-Name": "AnalogIO", "PIEZO-StepSize": "0.0050", "PRIME-ClearMode": "Pre-Exposure", "470 AO-MinVolts": "0.0000", "405 AO-SupportsTriggering": "1", "CameraChannelIndex": 0, "XYStage-StartSpeed": "500.0000", "COM9-StopBits": "2", "405 AO-Description": "NI DAC", "COM4-DelayBetweenCharsMs": "0.0000", "445 AO-Block voltage": "false", "520 AO-TriggerInputLine": "/Dev1/PFI0", "555 AO-Description": "NI DAC", "520 AO-TriggerSampleRate": "100000", "COM4-Verbose": "1", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM4-Name": "COM4", "405 Shutter-Volts": "0.0000", "Vincent-D3-Port": "COM1", "PRIME-PP 4 MIN ADU AFFECTED": "200", "470 Shutter-MinVolts": "0.0000", "PRIME-PP 3 ENABLED": "Yes", "470 AO-Description": "NI DAC", "PRIME-Description": "PVCAM API device adapter", "520 Shutter-MaxVolts": "5.0000", "520 Shutter-Volts": "0.0000", "528 AO-Sequenceable": "1", "PRIME-Binning": "1x1", "555 Shutter-MaxVolts": "5.0000", "528 Shutter-TriggerSequenceLength": "1000", "405 Shutter-Block voltage": "false", "XLIGHT Spin motor-State": "1", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "Vincent-D3-Time to open (ms)": "35.0000", "Vincent-D3-Time to close (ms)": "35.0000", "445 AO-TriggerSampleRate": "100000", "640 Shutter-IOChannel": "Dev1/ao14", "555 AO-Sequenceable": "1", "520 AO-Name": "AnalogIO", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "470 AO-Block voltage": "false", "COM1-Verbose": "1", "COM9-AnswerTimeout": "10000.0000", "528 Shutter-Name": "AnalogIO", "PRIME-Trigger Timeout (secs)": "10", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "NextFrame": 0, "470 AO-TriggerSequenceLength": "1000", "PRIME-TransposeXY": "0", "445 AO-TriggerSequenceLength": "1000", "COM1-Name": "COM1", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "COM9-DataBits": "8", "COM1-AnswerTimeout": "500.0000", "Camera": "", "TE2000-Version": "V2.50-V2.50-V2.10", "528 Shutter-Volts": "0.0000", "XLIGHT Hub-Name": "XLIGHT Hub", "PIEZO-Description": "Ludl stage driver adapter", "PositionName": "Pos29", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSampleRate": "100000", "PRIME-FanSpeedSetpoint": "High", "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "405 AO-TriggerSampleRate": "100000", "470 Shutter-Block voltage": "false", "470-State": "0", "COM3-Parity": "None", "405 AO-Volts": "0.0000", "PRIME-TransposeMirrorY": "0", "PRIME-ChipName": "GS144BSI", "470 AO-Volts": "0.0000", "PRIME-TransposeMirrorX": "0", "445 Shutter-SupportsTriggering": "0", "470 Shutter-SupportsTriggering": "0", "XLIGHT Hub-Description": "XLIGHT hub", "PRIME-PreampOffLimit": "10000", "470 AO-Sequenceable": "1", "528 Shutter-MaxVolts": "5.0000", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "Core-XYStage": "XYStage", "470 AO-TriggerSampleRate": "100000", "PRIME-Timing-PreTriggerDelayNs": "24672000.0000", "528-DA Device": "528 Shutter", "Focus-Description": "Nikon TE2000 focus adapter", "FilterBlock-State": "5", "520 AO-Demo": "No", "COM3-DelayBetweenCharsMs": "0.0000", "470 Shutter-Volts": "0.0000", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "PRIME-PP 2 THRESHOLD": "125", "PRIME-PP 4 THRESHOLD": "75", "XYStage-Acceleration": "4.0000", "470 Shutter-Demo": "No", "PRIME-Name": "PMPCIECam00", "640-DA Device": "640 Shutter", "405 AO-Block voltage": "false", "ElapsedTime-ms": 1206767, "PRIME-Offset": "100", "445 AO-IOChannel": "Dev1/ao1", "640 AO-MaxVolts": "5.0000", "PositionIndex": 29, "PRIME-PP 1 ENABLED": "Yes", "COM1-BaudRate": "9600", "640-State": "1", "COM3-Verbose": "1", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "LudlController-TransmissionDelay": "0", "PRIME-FullWellCapacity": "62000", "PRIME-SMARTStreamingEnabled": "No", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "640 Shutter-Description": "NI DAC", "528-Description": "DA device that is used as a shutter", "COM4-Description": "Serial port driver (boost:asio)", "405 Shutter-Description": "NI DAC", "TE2000-Name": "TE2000", "528 Shutter-Description": "NI DAC", "COM9-Description": "Serial port driver (boost:asio)", "LudlController-Name": "LudlController", "445 Shutter-TriggerSequenceLength": "1000", "PRIME-SerialNumber": "A18B203008", "445 AO-Volts": "0.0000", "PRIME-X-dimension": "1200", "PRIME-ShutterCloseDelay": "0", "XLIGHT Disk slider-State": "2", "Position": "Default", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 AO-IOChannel": "Dev1/ao5", "520 AO-IOChannel": "Dev1/ao4", "528 AO-TriggerSequenceLength": "1000", "405 Shutter-MaxVolts": "5.0000", "470-DA Device": "470 Shutter", "PRIME-FTCapable": "No", "Core-ChannelGroup": "Channels", "555 AO-SupportsTriggering": "0", "PRIME-Y-dimension": "1200", "Frame": 0, "555 Shutter-Sequenceable": "1", "PRIME-Timing-ExposureTimeNs": "499998000.0000", "Core-ImageProcessor": "", "528-State": "0", "520 AO-Block voltage": "false", "XLIGHT Excitation Wheel-Label": "QUAD", "640 Shutter-Demo": "No", "COM3-StopBits": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "520 Shutter-IOChannel": "Dev1/ao11", "Width": 1200, "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-SupportsTriggering": "0", "640 AO-TriggerSampleRate": "100000", "520-Description": "DA device that is used as a shutter", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "405 Shutter-Sequenceable": "1", "405 Shutter-SupportsTriggering": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-TriggerSampleRate": "100000", "640 Shutter-MaxVolts": "5.0000", "555 AO-MaxVolts": "5.0000", "520 AO-TriggerSequenceLength": "1000", "COM3-BaudRate": "9600", "COM9-BaudRate": "9600", "Vincent-D3-Channel #": "Ch. #1", "PRIME-PMode": "Normal", "OpticalPath-Label": "Left(100)", "BitDepth": 16, "555 AO-TriggerSequenceLength": "1000", "445 Shutter-Description": "NI DAC", "405 AO-Sequenceable": "1", "PRIME-AcquisitionMethod": "Callbacks", "555 AO-Name": "AnalogIO", "PRIME-ShutterMode": "Pre-Exposure", "COM3-Handshaking": "Off", "445 AO-SupportsTriggering": "0", "ROI": "0-0-1200-1200", "COM9-Verbose": "1", "PRIME-Color": "OFF", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "PRIME-ShutterOpenDelay": "0", "COM1-Parity": "None", "Slice": 7, "SlicePosition": 20, "COM9-DelayBetweenCharsMs": "0.0000", "555-Name": "DA Shutter", "PIEZO-LudlSingleAxisName": "B", "XLIGHT Emission Wheel-Label": "679/29 (A)", "640 Shutter-Name": "AnalogIO", "640 AO-Sequenceable": "1", "520 Shutter-Demo": "No", "528 Shutter-Demo": "No", "470-Name": "DA Shutter", "520-DA Device": "520 Shutter", "XYStage-Description": "Ludl XY stage driver adapter", "445 Shutter-MinVolts": "0.0000", "528 AO-TriggerSampleRate": "100000", "405 AO-MinVolts": "0.0000", "405 Shutter-TriggerSequenceLength": "1000", "Height": 1200, "555 AO-Block voltage": "false", "640 Shutter-Volts": "5.0000", "PixelType": "GRAY16", "445 Shutter-Block voltage": "false", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "520 Shutter-TriggerSequenceLength": "1000", "COM9-Name": "COM9", "Core-SLM": "", "555 AO-Volts": "0.0000", "555 Shutter-Block voltage": "false", "XYStage-Speed": "1800.0000", "520 AO-MaxVolts": "5.0000", "528 Shutter-SupportsTriggering": "0", "COM1-Description": "Serial port driver (boost:asio)", "PRIME-MetadataEnabled": "No", "520 Shutter-MinVolts": "0.0000", "470 Shutter-Sequenceable": "1", "PRIME-PreampDelay": "0", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-MinVolts": "0.0000", "ChannelIndex": 2, "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Core-AutoShutter": "1", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "Exposure-ms": 320, "PRIME-PP 2 ENABLED": "Yes", "640 Shutter-TriggerSampleRate": "100000", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "470 Shutter-IOChannel": "Dev1/ao10", "405 AO-Demo": "No", "PixelSizeUm": 1.1, "470 AO-Demo": "No", "470 Shutter-MaxVolts": "5.0000", "Vincent-D3-Name": "Vincent-D3", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FileName": "img_000000000_Il-A_007.tif", "LudlController-Reset": "Operate", "PRIME-Exposure": "320.00", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "445-Name": "DA Shutter", "555 Shutter-Name": "AnalogIO", "520 Shutter-TriggerSampleRate": "100000", "445 Shutter-IOChannel": "Dev1/ao9", "PRIME-TransposeCorrection": "0", "XYStage-StepSize": "0.1000", "445-Description": "DA device that is used as a shutter", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 AO-IOChannel": "Dev1/ao7", "520 Shutter-Name": "AnalogIO", "COM3-DataBits": "8", "640 AO-Volts": "4.5000", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "555 Shutter-IOChannel": "Dev1/ao13", "WaitInterval": null, "520 Shutter-Sequenceable": "1", "YPositionUm": -180, "445-State": "0", "445-DA Device": "445 Shutter", "PRIME-CircularBufferAutoSize": "ON", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "555 AO-TriggerSampleRate": "100000", "445 Shutter-Volts": "0.0000", "XLIGHT Hub-Port": "COM3", "Core-AutoFocus": "", "COM4-Handshaking": "Off", "528 Shutter-MinVolts": "0.0000", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "445 Shutter-Name": "AnalogIO", "528 Shutter-Sequenceable": "1", "XYStage-TransposeMirrorX": "0", "XYStage-TransposeMirrorY": "0", "PRIME-ReadoutRate": "100MHz 16bit", "XYStage-Name": "Stage", "640 AO-SupportsTriggering": "0", "640-Name": "DA Shutter", "640 Shutter-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "640 AO-Block voltage": "false", "445 Shutter-Sequenceable": "1", "470 AO-MaxVolts": "5.0000", "COM4-BaudRate": "9600", "470-Description": "DA device that is used as a shutter", "520 Shutter-Description": "NI DAC", "PRIME-CCDTemperature": "-14.8500", "Time": "2019-12-18 23:11:06 -0500", "640 AO-TriggerSequenceLength": "1000", "470 AO-Name": "AnalogIO", "XLIGHT Disk slider-Label": "Disk Pos 40um", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "Binning": "1x1", "TE2000-ModelType": "1", "COM3-AnswerTimeout": "500.0000", "555 Shutter-SupportsTriggering": "0", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "TE2000-Port": "COM4", "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Description": "NI DAC", "COM4-AnswerTimeout": "500.0000", "COM1-DelayBetweenCharsMs": "0.0000", "FilterBlock-Label": "Confocal", "445 AO-Name": "AnalogIO", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-Timing-ReadoutTimeNs": "24672000.0000", "528 AO-Demo": "No", "520 AO-Sequenceable": "1", "555-Description": "DA device that is used as a shutter", "405-DA Device": "405 Shutter", "405 AO-TriggerSequenceLength": "1000", "640 AO-MinVolts": "0.0000", "640 Shutter-Block voltage": "false", "FrameIndex": 0, "PRIME-Port": "CMOS", "555 AO-MinVolts": "0.0000", "COM3-Description": "Serial port driver (boost:asio)", "528 AO-Description": "NI DAC", "555 AO-IOChannel": "Dev1/ao6", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "555 AO-TriggerInputLine": "/Dev1/PFI0", "PRIME-ActualInterval-ms": "390.5200", "LudlController-Port": "COM9", "445 Shutter-Demo": "No", "PRIME-Trigger-Expose Out-Mux": "1", "SliceIndex": 7, "PRIME-CCDTemperatureSetPoint": "-15.0000", "405 Shutter-MinVolts": "0.0000", "PRIME-ExposeOutMode": "First Row", "AxisPositions": null, "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Volts": "0.0000", "528-Name": "DA Shutter", "555-State": "0", "XLIGHT Dichroic-Label": "Di03-R635-t1", "445 AO-Sequenceable": "1", "405-Name": "DA Shutter", "405 AO-IOChannel": "Dev1/ao0", "Core-Camera": "PRIME", "Focus-Name": "Focus", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "405 Shutter-IOChannel": "Dev1/ao8", "640 AO-Demo": "No", "640 Shutter-SupportsTriggering": "0", "445 AO-Demo": "No", "555-DA Device": "555 Shutter", "470 Shutter-Description": "NI DAC", "528 AO-Volts": "0.0000", "COM1-DataBits": "8", "Vincent-D3-Address": "x", "520 AO-SupportsTriggering": "0", "405-Description": "DA device that is used as a shutter", "640 Shutter-MinVolts": "0.0000", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "405 AO-Name": "AnalogIO", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "640 AO-TriggerInputLine": "/Dev1/PFI0", "405 AO-TriggerInputLine": "/Dev1/PFI0", "640-Description": "DA device that is used as a shutter", "445 Shutter-TriggerSampleRate": "100000", "470 Shutter-TriggerSequenceLength": "1000", "COM1-StopBits": "1", "520 Shutter-SupportsTriggering": "0", "405-State": "0", "Core-Initialize": "1", "XLIGHT Emission Wheel-State": "3", "520 AO-Description": "NI DAC", "528 AO-TriggerInputLine": "/Dev1/PFI0", "445 Shutter-MaxVolts": "5.0000", "COM9-Handshaking": "Off", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "XLIGHT Spin motor-Label": "On (spinning)", "PRIME-PP 5 ENABLED": "No", "UUID": "87b19190-d89b-4667-87b8-790d85a8c207", "PRIME-TriggerMode": "Internal Trigger", "COM1-Handshaking": "Off", "PRIME-Timing-PostTriggerDelayNs": "49344000.0000", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "Focus-Position": "17741.5000", "528 Shutter-Block voltage": "false", "Channel": "Il-A", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "555 Shutter-Demo": "No", "405 Shutter-Demo": "No", "528 AO-Name": "AnalogIO", "520-State": "0", "Core-Galvo": "", "405 Shutter-TriggerSampleRate": "100000", "Focus-FocusStepSize_(nm)": "100", "PRIME-PixelType": "16bit", "COM4-DataBits": "8", "470 AO-IOChannel": "Dev1/ao2", "520 AO-MinVolts": "0.0000", "COM9-Parity": "None", "PRIME-CircularBufferEnabled": "ON", "PRIME-PP 6 Reset": "No", "640 AO-Description": "NI DAC", "Source": "PRIME", "445 AO-MinVolts": "0.0000", "PIEZO-Name": "Stage", "445 AO-Description": "NI DAC", "PRIME-CircularBufferFrameCount": "46", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "520 AO-Volts": "0.0000", "528 Shutter-TriggerSampleRate": "100000", "XPositionUm": 15712.1, "OpticalPath-State": "4", "PRIME-PP 3 THRESHOLD": "80", "PRIME-PVCAM Version": "3.7.1", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Gain": "1-HDR", "640 Shutter-TriggerSequenceLength": "1000", "528 AO-SupportsTriggering": "0", "445 AO-TriggerInputLine": "/Dev1/PFI0", "OpticalPath-Name": "OpticalPath", "PRIME-PP 4 ENABLED": "Yes", "PRIME-PVCAM Adapter Version": "1.3.29", "COM3-Name": "COM3", "405 AO-MaxVolts": "5.0000", "520 Shutter-Block voltage": "false", "PRIME-Timing-ClearingTimeNs": "49344000.0000", "Core-Focus": "PIEZO", "528 AO-Block voltage": "false", "TE2000-Description": "Nikon TE2000 hub", "528 AO-MinVolts": "0.0000", "COM4-Parity": "None", "Summary": { "Slices": 11, "ChContrastMin": [ 0, 0, 0, 0, 0, 0 ], "PixelAspect": 1, "SlicesFirst": true, "Interval_ms": 0, "Time": "2019-12-18 23:10:50 -0500", "Prefix": "seq_5", "InitialPositionList": [ { "Label": "Pos0", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.21], "Focus": [17741.5], "XYStage": [ 44542.200000000004, 31636.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos1", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.22], "Focus": [17741.4], "XYStage": [ 43801.100000000006, 31637.9 ] }, "GridColumnIndex": 0 }, { "Label": "Pos2", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.5], "XYStage": [ 38561.3, 32040.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos3", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.185], "Focus": [17741.3], "XYStage": [ 37698.4, 32041.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos4", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.16], "Focus": [17741.5], "XYStage": [ 32375.4, 31758.9 ] }, "GridColumnIndex": 0 }, { "Label": "Pos5", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.17000000000002], "Focus": [17741.3], "XYStage": [ 31770.7, 31758.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos6", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.4], "XYStage": [ 26536.800000000003, 31068.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos7", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.205], "Focus": [17741.4], "XYStage": [ 25420.2, 31068.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos8", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.85], "Focus": [17741.3], "XYStage": [ 21059, 31508 ] }, "GridColumnIndex": 0 }, { "Label": "Pos9", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.82], "Focus": [17741.3], "XYStage": [ 19731.9, 31506.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos10", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.79], "Focus": [17741.4], "XYStage": [ 14528.5, 32007.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos11", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.83], "Focus": [17741.2], "XYStage": [ 13830.300000000001, 32009.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos12", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.79], "Focus": [17741.3], "XYStage": [ 8775.300000000001, 32017.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos13", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.815], "Focus": [17741.3], "XYStage": [ 8109.200000000001, 32008.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos14", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.805], "Focus": [17741.3], "XYStage": [ 3483.3, 31908.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos15", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.82], "Focus": [17741.2], "XYStage": [ 2686.8, 31903.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos16", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [211.48000000000002], "Focus": [17741.1], "XYStage": [ -1978.9, 31973.600000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos17", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [211.51500000000001], "Focus": [17741.1], "XYStage": [ -2957.8, 31982.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos18", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.195], "Focus": [17741.5], "XYStage": [ 47747.4, -576.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos19", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.23000000000002], "Focus": [17741.6], "XYStage": [ 47053.9, -576.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos20", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.205], "Focus": [17741.4], "XYStage": [ 41871.200000000004, -479.8 ] }, "GridColumnIndex": 0 }, { "Label": "Pos21", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.4], "XYStage": [ 40882.600000000006, -392 ] }, "GridColumnIndex": 0 }, { "Label": "Pos22", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.24], "Focus": [17741.4], "XYStage": [ 35905.6, -135.3 ] }, "GridColumnIndex": 0 }, { "Label": "Pos23", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.195], "Focus": [17741.5], "XYStage": [ 34990.9, -132.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos24", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.46], "Focus": [17741.4], "XYStage": [ 29678.100000000002, -958.1 ] }, "GridColumnIndex": 0 }, { "Label": "Pos25", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.44], "Focus": [17741.5], "XYStage": [ 28983.600000000002, -939.4000000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos26", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.475], "Focus": [17741.5], "XYStage": [ 23019.7, -122 ] }, "GridColumnIndex": 0 }, { "Label": "Pos27", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.44], "Focus": [17741.7], "XYStage": [ 22428, -116.80000000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos28", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.46], "Focus": [17741.7], "XYStage": [ 16364.900000000001, -181.10000000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos29", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.48000000000002], "Focus": [17741.5], "XYStage": [ 15712.1, -180 ] }, "GridColumnIndex": 0 }, { "Label": "Pos30", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.45000000000002], "Focus": [17741.7], "XYStage": [ 10346.1, 77.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos31", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.49], "Focus": [17741.4], "XYStage": [ 9755.5, 85.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos32", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.475], "Focus": [17741.5], "XYStage": [ 4775.5, 293.90000000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos33", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.445], "Focus": [17741.4], "XYStage": [ 3954.9, 293.90000000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos34", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.455], "Focus": [17741.4], "XYStage": [ -1324.9, 545.6 ] }, "GridColumnIndex": 0 }, { "Label": "Pos35", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.445], "Focus": [17741.3], "XYStage": [ -2097, 576.6 ] }, "GridColumnIndex": 0 } ], "z-step_um": 10, "GridRow": 0, "BitDepth": 16, "Depth": 2, "Channels": 6, "Source": "Micro-Manager", "ChColors": [ -16711681, -256, -65281, -1, -1, -16776961 ], "MetadataVersion": 10, "KeepShutterOpenChannels": true, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536, 65536 ], "CustomIntervals_ms": [], "Height": 1200, "MicroManagerVersion": "1.4.23 20180219", "GridColumn": 0, "UUID": "67598d21-5db5-4842-ab60-f506f7b42c7c", "PixelType": "GRAY16", "Width": 1200, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\Yan\\20191218-YC113-S7S8-miseq", "Frames": 1, "Comment": "", "UserName": "Nikon", "PixelSize_um": 1.1, "ROI": [ 0, 0, 1200, 1200 ], "Date": "2019-12-18", "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC", "P-DAPI-high" ], "KeepShutterOpenSlices": true, "IJType": 1, "ComputerName": "ZadorConfocal2", "Positions": 36, "PositionIndex": 29, "CameraTimeout": 20000, "TimeFirst": false }, "640 AO-Name": "AnalogIO", "Vincent-D3-Command": "Close" }, "FrameKey-0-2-8": { "COM4-StopBits": "1", "528 AO-MaxVolts": "5.0000", "XLIGHT Excitation Wheel-State": "1", "470 Shutter-Name": "AnalogIO", "Core-Shutter": "640", "555 AO-Demo": "No", "XLIGHT Dichroic-State": "2", "XYStage-StepSize-Y": "0.1000", "ZPositionUm": 248.48000000000002, "XYStage-StepSize-X": "0.1000", "PRIME-FirmwareVersion": "20.28", "PRIME-ClearCycles": "2", "520-Name": "DA Shutter", "405 Shutter-Name": "AnalogIO", "PIEZO-StepSize": "0.0050", "PRIME-ClearMode": "Pre-Exposure", "470 AO-MinVolts": "0.0000", "405 AO-SupportsTriggering": "1", "CameraChannelIndex": 0, "XYStage-StartSpeed": "500.0000", "COM9-StopBits": "2", "405 AO-Description": "NI DAC", "COM4-DelayBetweenCharsMs": "0.0000", "445 AO-Block voltage": "false", "520 AO-TriggerInputLine": "/Dev1/PFI0", "555 AO-Description": "NI DAC", "520 AO-TriggerSampleRate": "100000", "COM4-Verbose": "1", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM4-Name": "COM4", "405 Shutter-Volts": "0.0000", "Vincent-D3-Port": "COM1", "PRIME-PP 4 MIN ADU AFFECTED": "200", "470 Shutter-MinVolts": "0.0000", "PRIME-PP 3 ENABLED": "Yes", "470 AO-Description": "NI DAC", "PRIME-Description": "PVCAM API device adapter", "520 Shutter-MaxVolts": "5.0000", "520 Shutter-Volts": "0.0000", "528 AO-Sequenceable": "1", "PRIME-Binning": "1x1", "555 Shutter-MaxVolts": "5.0000", "528 Shutter-TriggerSequenceLength": "1000", "405 Shutter-Block voltage": "false", "XLIGHT Spin motor-State": "1", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "Vincent-D3-Time to open (ms)": "35.0000", "Vincent-D3-Time to close (ms)": "35.0000", "445 AO-TriggerSampleRate": "100000", "640 Shutter-IOChannel": "Dev1/ao14", "555 AO-Sequenceable": "1", "520 AO-Name": "AnalogIO", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "470 AO-Block voltage": "false", "COM1-Verbose": "1", "COM9-AnswerTimeout": "10000.0000", "528 Shutter-Name": "AnalogIO", "PRIME-Trigger Timeout (secs)": "10", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "NextFrame": 0, "470 AO-TriggerSequenceLength": "1000", "PRIME-TransposeXY": "0", "445 AO-TriggerSequenceLength": "1000", "COM1-Name": "COM1", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "COM9-DataBits": "8", "COM1-AnswerTimeout": "500.0000", "Camera": "", "TE2000-Version": "V2.50-V2.50-V2.10", "528 Shutter-Volts": "0.0000", "XLIGHT Hub-Name": "XLIGHT Hub", "PIEZO-Description": "Ludl stage driver adapter", "PositionName": "Pos29", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSampleRate": "100000", "PRIME-FanSpeedSetpoint": "High", "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "405 AO-TriggerSampleRate": "100000", "470 Shutter-Block voltage": "false", "470-State": "0", "COM3-Parity": "None", "405 AO-Volts": "0.0000", "PRIME-TransposeMirrorY": "0", "PRIME-ChipName": "GS144BSI", "470 AO-Volts": "0.0000", "PRIME-TransposeMirrorX": "0", "445 Shutter-SupportsTriggering": "0", "470 Shutter-SupportsTriggering": "0", "XLIGHT Hub-Description": "XLIGHT hub", "PRIME-PreampOffLimit": "10000", "470 AO-Sequenceable": "1", "528 Shutter-MaxVolts": "5.0000", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "Core-XYStage": "XYStage", "470 AO-TriggerSampleRate": "100000", "PRIME-Timing-PreTriggerDelayNs": "24672000.0000", "528-DA Device": "528 Shutter", "Focus-Description": "Nikon TE2000 focus adapter", "FilterBlock-State": "5", "520 AO-Demo": "No", "COM3-DelayBetweenCharsMs": "0.0000", "470 Shutter-Volts": "0.0000", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "PRIME-PP 2 THRESHOLD": "125", "PRIME-PP 4 THRESHOLD": "75", "XYStage-Acceleration": "4.0000", "470 Shutter-Demo": "No", "PRIME-Name": "PMPCIECam00", "640-DA Device": "640 Shutter", "405 AO-Block voltage": "false", "ElapsedTime-ms": 1207324, "PRIME-Offset": "100", "445 AO-IOChannel": "Dev1/ao1", "640 AO-MaxVolts": "5.0000", "PositionIndex": 29, "PRIME-PP 1 ENABLED": "Yes", "COM1-BaudRate": "9600", "640-State": "1", "COM3-Verbose": "1", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "LudlController-TransmissionDelay": "0", "PRIME-FullWellCapacity": "62000", "PRIME-SMARTStreamingEnabled": "No", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "640 Shutter-Description": "NI DAC", "528-Description": "DA device that is used as a shutter", "COM4-Description": "Serial port driver (boost:asio)", "405 Shutter-Description": "NI DAC", "TE2000-Name": "TE2000", "528 Shutter-Description": "NI DAC", "COM9-Description": "Serial port driver (boost:asio)", "LudlController-Name": "LudlController", "445 Shutter-TriggerSequenceLength": "1000", "PRIME-SerialNumber": "A18B203008", "445 AO-Volts": "0.0000", "PRIME-X-dimension": "1200", "PRIME-ShutterCloseDelay": "0", "XLIGHT Disk slider-State": "2", "Position": "Default", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 AO-IOChannel": "Dev1/ao5", "520 AO-IOChannel": "Dev1/ao4", "528 AO-TriggerSequenceLength": "1000", "405 Shutter-MaxVolts": "5.0000", "470-DA Device": "470 Shutter", "PRIME-FTCapable": "No", "Core-ChannelGroup": "Channels", "555 AO-SupportsTriggering": "0", "PRIME-Y-dimension": "1200", "Frame": 0, "555 Shutter-Sequenceable": "1", "PRIME-Timing-ExposureTimeNs": "499998000.0000", "Core-ImageProcessor": "", "528-State": "0", "520 AO-Block voltage": "false", "XLIGHT Excitation Wheel-Label": "QUAD", "640 Shutter-Demo": "No", "COM3-StopBits": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "520 Shutter-IOChannel": "Dev1/ao11", "Width": 1200, "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-SupportsTriggering": "0", "640 AO-TriggerSampleRate": "100000", "520-Description": "DA device that is used as a shutter", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "405 Shutter-Sequenceable": "1", "405 Shutter-SupportsTriggering": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-TriggerSampleRate": "100000", "640 Shutter-MaxVolts": "5.0000", "555 AO-MaxVolts": "5.0000", "520 AO-TriggerSequenceLength": "1000", "COM3-BaudRate": "9600", "COM9-BaudRate": "9600", "Vincent-D3-Channel #": "Ch. #1", "PRIME-PMode": "Normal", "OpticalPath-Label": "Left(100)", "BitDepth": 16, "555 AO-TriggerSequenceLength": "1000", "445 Shutter-Description": "NI DAC", "405 AO-Sequenceable": "1", "PRIME-AcquisitionMethod": "Callbacks", "555 AO-Name": "AnalogIO", "PRIME-ShutterMode": "Pre-Exposure", "COM3-Handshaking": "Off", "445 AO-SupportsTriggering": "0", "ROI": "0-0-1200-1200", "COM9-Verbose": "1", "PRIME-Color": "OFF", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "PRIME-ShutterOpenDelay": "0", "COM1-Parity": "None", "Slice": 8, "SlicePosition": 30, "COM9-DelayBetweenCharsMs": "0.0000", "555-Name": "DA Shutter", "PIEZO-LudlSingleAxisName": "B", "XLIGHT Emission Wheel-Label": "679/29 (A)", "640 Shutter-Name": "AnalogIO", "640 AO-Sequenceable": "1", "520 Shutter-Demo": "No", "528 Shutter-Demo": "No", "470-Name": "DA Shutter", "520-DA Device": "520 Shutter", "XYStage-Description": "Ludl XY stage driver adapter", "445 Shutter-MinVolts": "0.0000", "528 AO-TriggerSampleRate": "100000", "405 AO-MinVolts": "0.0000", "405 Shutter-TriggerSequenceLength": "1000", "Height": 1200, "555 AO-Block voltage": "false", "640 Shutter-Volts": "5.0000", "PixelType": "GRAY16", "445 Shutter-Block voltage": "false", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "520 Shutter-TriggerSequenceLength": "1000", "COM9-Name": "COM9", "Core-SLM": "", "555 AO-Volts": "0.0000", "555 Shutter-Block voltage": "false", "XYStage-Speed": "1800.0000", "520 AO-MaxVolts": "5.0000", "528 Shutter-SupportsTriggering": "0", "COM1-Description": "Serial port driver (boost:asio)", "PRIME-MetadataEnabled": "No", "520 Shutter-MinVolts": "0.0000", "470 Shutter-Sequenceable": "1", "PRIME-PreampDelay": "0", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-MinVolts": "0.0000", "ChannelIndex": 2, "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Core-AutoShutter": "1", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "Exposure-ms": 320, "PRIME-PP 2 ENABLED": "Yes", "640 Shutter-TriggerSampleRate": "100000", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "470 Shutter-IOChannel": "Dev1/ao10", "405 AO-Demo": "No", "PixelSizeUm": 1.1, "470 AO-Demo": "No", "470 Shutter-MaxVolts": "5.0000", "Vincent-D3-Name": "Vincent-D3", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FileName": "img_000000000_Il-A_008.tif", "LudlController-Reset": "Operate", "PRIME-Exposure": "320.00", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "445-Name": "DA Shutter", "555 Shutter-Name": "AnalogIO", "520 Shutter-TriggerSampleRate": "100000", "445 Shutter-IOChannel": "Dev1/ao9", "PRIME-TransposeCorrection": "0", "XYStage-StepSize": "0.1000", "445-Description": "DA device that is used as a shutter", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 AO-IOChannel": "Dev1/ao7", "520 Shutter-Name": "AnalogIO", "COM3-DataBits": "8", "640 AO-Volts": "4.5000", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "555 Shutter-IOChannel": "Dev1/ao13", "WaitInterval": null, "520 Shutter-Sequenceable": "1", "YPositionUm": -180, "445-State": "0", "445-DA Device": "445 Shutter", "PRIME-CircularBufferAutoSize": "ON", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "555 AO-TriggerSampleRate": "100000", "445 Shutter-Volts": "0.0000", "XLIGHT Hub-Port": "COM3", "Core-AutoFocus": "", "COM4-Handshaking": "Off", "528 Shutter-MinVolts": "0.0000", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "445 Shutter-Name": "AnalogIO", "528 Shutter-Sequenceable": "1", "XYStage-TransposeMirrorX": "0", "XYStage-TransposeMirrorY": "0", "PRIME-ReadoutRate": "100MHz 16bit", "XYStage-Name": "Stage", "640 AO-SupportsTriggering": "0", "640-Name": "DA Shutter", "640 Shutter-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "640 AO-Block voltage": "false", "445 Shutter-Sequenceable": "1", "470 AO-MaxVolts": "5.0000", "COM4-BaudRate": "9600", "470-Description": "DA device that is used as a shutter", "520 Shutter-Description": "NI DAC", "PRIME-CCDTemperature": "-14.8500", "Time": "2019-12-18 23:11:07 -0500", "640 AO-TriggerSequenceLength": "1000", "470 AO-Name": "AnalogIO", "XLIGHT Disk slider-Label": "Disk Pos 40um", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "Binning": "1x1", "TE2000-ModelType": "1", "COM3-AnswerTimeout": "500.0000", "555 Shutter-SupportsTriggering": "0", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "TE2000-Port": "COM4", "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Description": "NI DAC", "COM4-AnswerTimeout": "500.0000", "COM1-DelayBetweenCharsMs": "0.0000", "FilterBlock-Label": "Confocal", "445 AO-Name": "AnalogIO", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-Timing-ReadoutTimeNs": "24672000.0000", "528 AO-Demo": "No", "520 AO-Sequenceable": "1", "555-Description": "DA device that is used as a shutter", "405-DA Device": "405 Shutter", "405 AO-TriggerSequenceLength": "1000", "640 AO-MinVolts": "0.0000", "640 Shutter-Block voltage": "false", "FrameIndex": 0, "PRIME-Port": "CMOS", "555 AO-MinVolts": "0.0000", "COM3-Description": "Serial port driver (boost:asio)", "528 AO-Description": "NI DAC", "555 AO-IOChannel": "Dev1/ao6", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "555 AO-TriggerInputLine": "/Dev1/PFI0", "PRIME-ActualInterval-ms": "390.5200", "LudlController-Port": "COM9", "445 Shutter-Demo": "No", "PRIME-Trigger-Expose Out-Mux": "1", "SliceIndex": 8, "PRIME-CCDTemperatureSetPoint": "-15.0000", "405 Shutter-MinVolts": "0.0000", "PRIME-ExposeOutMode": "First Row", "AxisPositions": null, "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Volts": "0.0000", "528-Name": "DA Shutter", "555-State": "0", "XLIGHT Dichroic-Label": "Di03-R635-t1", "445 AO-Sequenceable": "1", "405-Name": "DA Shutter", "405 AO-IOChannel": "Dev1/ao0", "Core-Camera": "PRIME", "Focus-Name": "Focus", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "405 Shutter-IOChannel": "Dev1/ao8", "640 AO-Demo": "No", "640 Shutter-SupportsTriggering": "0", "445 AO-Demo": "No", "555-DA Device": "555 Shutter", "470 Shutter-Description": "NI DAC", "528 AO-Volts": "0.0000", "COM1-DataBits": "8", "Vincent-D3-Address": "x", "520 AO-SupportsTriggering": "0", "405-Description": "DA device that is used as a shutter", "640 Shutter-MinVolts": "0.0000", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "405 AO-Name": "AnalogIO", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "640 AO-TriggerInputLine": "/Dev1/PFI0", "405 AO-TriggerInputLine": "/Dev1/PFI0", "640-Description": "DA device that is used as a shutter", "445 Shutter-TriggerSampleRate": "100000", "470 Shutter-TriggerSequenceLength": "1000", "COM1-StopBits": "1", "520 Shutter-SupportsTriggering": "0", "405-State": "0", "Core-Initialize": "1", "XLIGHT Emission Wheel-State": "3", "520 AO-Description": "NI DAC", "528 AO-TriggerInputLine": "/Dev1/PFI0", "445 Shutter-MaxVolts": "5.0000", "COM9-Handshaking": "Off", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "XLIGHT Spin motor-Label": "On (spinning)", "PRIME-PP 5 ENABLED": "No", "UUID": "cfe3eed0-edfc-42a6-bfff-8f692b9b3b11", "PRIME-TriggerMode": "Internal Trigger", "COM1-Handshaking": "Off", "PRIME-Timing-PostTriggerDelayNs": "49344000.0000", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "Focus-Position": "17741.5000", "528 Shutter-Block voltage": "false", "Channel": "Il-A", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "555 Shutter-Demo": "No", "405 Shutter-Demo": "No", "528 AO-Name": "AnalogIO", "520-State": "0", "Core-Galvo": "", "405 Shutter-TriggerSampleRate": "100000", "Focus-FocusStepSize_(nm)": "100", "PRIME-PixelType": "16bit", "COM4-DataBits": "8", "470 AO-IOChannel": "Dev1/ao2", "520 AO-MinVolts": "0.0000", "COM9-Parity": "None", "PRIME-CircularBufferEnabled": "ON", "PRIME-PP 6 Reset": "No", "640 AO-Description": "NI DAC", "Source": "PRIME", "445 AO-MinVolts": "0.0000", "PIEZO-Name": "Stage", "445 AO-Description": "NI DAC", "PRIME-CircularBufferFrameCount": "46", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "520 AO-Volts": "0.0000", "528 Shutter-TriggerSampleRate": "100000", "XPositionUm": 15712.1, "OpticalPath-State": "4", "PRIME-PP 3 THRESHOLD": "80", "PRIME-PVCAM Version": "3.7.1", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Gain": "1-HDR", "640 Shutter-TriggerSequenceLength": "1000", "528 AO-SupportsTriggering": "0", "445 AO-TriggerInputLine": "/Dev1/PFI0", "OpticalPath-Name": "OpticalPath", "PRIME-PP 4 ENABLED": "Yes", "PRIME-PVCAM Adapter Version": "1.3.29", "COM3-Name": "COM3", "405 AO-MaxVolts": "5.0000", "520 Shutter-Block voltage": "false", "PRIME-Timing-ClearingTimeNs": "49344000.0000", "Core-Focus": "PIEZO", "528 AO-Block voltage": "false", "TE2000-Description": "Nikon TE2000 hub", "528 AO-MinVolts": "0.0000", "COM4-Parity": "None", "Summary": { "Slices": 11, "ChContrastMin": [ 0, 0, 0, 0, 0, 0 ], "PixelAspect": 1, "SlicesFirst": true, "Interval_ms": 0, "Time": "2019-12-18 23:10:50 -0500", "Prefix": "seq_5", "InitialPositionList": [ { "Label": "Pos0", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.21], "Focus": [17741.5], "XYStage": [ 44542.200000000004, 31636.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos1", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.22], "Focus": [17741.4], "XYStage": [ 43801.100000000006, 31637.9 ] }, "GridColumnIndex": 0 }, { "Label": "Pos2", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.5], "XYStage": [ 38561.3, 32040.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos3", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.185], "Focus": [17741.3], "XYStage": [ 37698.4, 32041.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos4", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.16], "Focus": [17741.5], "XYStage": [ 32375.4, 31758.9 ] }, "GridColumnIndex": 0 }, { "Label": "Pos5", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.17000000000002], "Focus": [17741.3], "XYStage": [ 31770.7, 31758.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos6", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.4], "XYStage": [ 26536.800000000003, 31068.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos7", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.205], "Focus": [17741.4], "XYStage": [ 25420.2, 31068.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos8", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.85], "Focus": [17741.3], "XYStage": [ 21059, 31508 ] }, "GridColumnIndex": 0 }, { "Label": "Pos9", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.82], "Focus": [17741.3], "XYStage": [ 19731.9, 31506.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos10", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.79], "Focus": [17741.4], "XYStage": [ 14528.5, 32007.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos11", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.83], "Focus": [17741.2], "XYStage": [ 13830.300000000001, 32009.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos12", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.79], "Focus": [17741.3], "XYStage": [ 8775.300000000001, 32017.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos13", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.815], "Focus": [17741.3], "XYStage": [ 8109.200000000001, 32008.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos14", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.805], "Focus": [17741.3], "XYStage": [ 3483.3, 31908.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos15", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.82], "Focus": [17741.2], "XYStage": [ 2686.8, 31903.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos16", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [211.48000000000002], "Focus": [17741.1], "XYStage": [ -1978.9, 31973.600000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos17", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [211.51500000000001], "Focus": [17741.1], "XYStage": [ -2957.8, 31982.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos18", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.195], "Focus": [17741.5], "XYStage": [ 47747.4, -576.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos19", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.23000000000002], "Focus": [17741.6], "XYStage": [ 47053.9, -576.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos20", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.205], "Focus": [17741.4], "XYStage": [ 41871.200000000004, -479.8 ] }, "GridColumnIndex": 0 }, { "Label": "Pos21", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.4], "XYStage": [ 40882.600000000006, -392 ] }, "GridColumnIndex": 0 }, { "Label": "Pos22", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.24], "Focus": [17741.4], "XYStage": [ 35905.6, -135.3 ] }, "GridColumnIndex": 0 }, { "Label": "Pos23", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.195], "Focus": [17741.5], "XYStage": [ 34990.9, -132.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos24", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.46], "Focus": [17741.4], "XYStage": [ 29678.100000000002, -958.1 ] }, "GridColumnIndex": 0 }, { "Label": "Pos25", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.44], "Focus": [17741.5], "XYStage": [ 28983.600000000002, -939.4000000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos26", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.475], "Focus": [17741.5], "XYStage": [ 23019.7, -122 ] }, "GridColumnIndex": 0 }, { "Label": "Pos27", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.44], "Focus": [17741.7], "XYStage": [ 22428, -116.80000000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos28", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.46], "Focus": [17741.7], "XYStage": [ 16364.900000000001, -181.10000000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos29", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.48000000000002], "Focus": [17741.5], "XYStage": [ 15712.1, -180 ] }, "GridColumnIndex": 0 }, { "Label": "Pos30", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.45000000000002], "Focus": [17741.7], "XYStage": [ 10346.1, 77.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos31", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.49], "Focus": [17741.4], "XYStage": [ 9755.5, 85.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos32", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.475], "Focus": [17741.5], "XYStage": [ 4775.5, 293.90000000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos33", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.445], "Focus": [17741.4], "XYStage": [ 3954.9, 293.90000000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos34", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.455], "Focus": [17741.4], "XYStage": [ -1324.9, 545.6 ] }, "GridColumnIndex": 0 }, { "Label": "Pos35", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.445], "Focus": [17741.3], "XYStage": [ -2097, 576.6 ] }, "GridColumnIndex": 0 } ], "z-step_um": 10, "GridRow": 0, "BitDepth": 16, "Depth": 2, "Channels": 6, "Source": "Micro-Manager", "ChColors": [ -16711681, -256, -65281, -1, -1, -16776961 ], "MetadataVersion": 10, "KeepShutterOpenChannels": true, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536, 65536 ], "CustomIntervals_ms": [], "Height": 1200, "MicroManagerVersion": "1.4.23 20180219", "GridColumn": 0, "UUID": "67598d21-5db5-4842-ab60-f506f7b42c7c", "PixelType": "GRAY16", "Width": 1200, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\Yan\\20191218-YC113-S7S8-miseq", "Frames": 1, "Comment": "", "UserName": "Nikon", "PixelSize_um": 1.1, "ROI": [ 0, 0, 1200, 1200 ], "Date": "2019-12-18", "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC", "P-DAPI-high" ], "KeepShutterOpenSlices": true, "IJType": 1, "ComputerName": "ZadorConfocal2", "Positions": 36, "PositionIndex": 29, "CameraTimeout": 20000, "TimeFirst": false }, "640 AO-Name": "AnalogIO", "Vincent-D3-Command": "Close" }, "FrameKey-0-2-9": { "COM4-StopBits": "1", "528 AO-MaxVolts": "5.0000", "XLIGHT Excitation Wheel-State": "1", "470 Shutter-Name": "AnalogIO", "Core-Shutter": "640", "555 AO-Demo": "No", "XLIGHT Dichroic-State": "2", "XYStage-StepSize-Y": "0.1000", "ZPositionUm": 258.48, "XYStage-StepSize-X": "0.1000", "PRIME-FirmwareVersion": "20.28", "PRIME-ClearCycles": "2", "520-Name": "DA Shutter", "405 Shutter-Name": "AnalogIO", "PIEZO-StepSize": "0.0050", "PRIME-ClearMode": "Pre-Exposure", "470 AO-MinVolts": "0.0000", "405 AO-SupportsTriggering": "1", "CameraChannelIndex": 0, "XYStage-StartSpeed": "500.0000", "COM9-StopBits": "2", "405 AO-Description": "NI DAC", "COM4-DelayBetweenCharsMs": "0.0000", "445 AO-Block voltage": "false", "520 AO-TriggerInputLine": "/Dev1/PFI0", "555 AO-Description": "NI DAC", "520 AO-TriggerSampleRate": "100000", "COM4-Verbose": "1", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM4-Name": "COM4", "405 Shutter-Volts": "0.0000", "Vincent-D3-Port": "COM1", "PRIME-PP 4 MIN ADU AFFECTED": "200", "470 Shutter-MinVolts": "0.0000", "PRIME-PP 3 ENABLED": "Yes", "470 AO-Description": "NI DAC", "PRIME-Description": "PVCAM API device adapter", "520 Shutter-MaxVolts": "5.0000", "520 Shutter-Volts": "0.0000", "528 AO-Sequenceable": "1", "PRIME-Binning": "1x1", "555 Shutter-MaxVolts": "5.0000", "528 Shutter-TriggerSequenceLength": "1000", "405 Shutter-Block voltage": "false", "XLIGHT Spin motor-State": "1", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "Vincent-D3-Time to open (ms)": "35.0000", "Vincent-D3-Time to close (ms)": "35.0000", "445 AO-TriggerSampleRate": "100000", "640 Shutter-IOChannel": "Dev1/ao14", "555 AO-Sequenceable": "1", "520 AO-Name": "AnalogIO", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "470 AO-Block voltage": "false", "COM1-Verbose": "1", "COM9-AnswerTimeout": "10000.0000", "528 Shutter-Name": "AnalogIO", "PRIME-Trigger Timeout (secs)": "10", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "NextFrame": 0, "470 AO-TriggerSequenceLength": "1000", "PRIME-TransposeXY": "0", "445 AO-TriggerSequenceLength": "1000", "COM1-Name": "COM1", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "COM9-DataBits": "8", "COM1-AnswerTimeout": "500.0000", "Camera": "", "TE2000-Version": "V2.50-V2.50-V2.10", "528 Shutter-Volts": "0.0000", "XLIGHT Hub-Name": "XLIGHT Hub", "PIEZO-Description": "Ludl stage driver adapter", "PositionName": "Pos29", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSampleRate": "100000", "PRIME-FanSpeedSetpoint": "High", "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "405 AO-TriggerSampleRate": "100000", "470 Shutter-Block voltage": "false", "470-State": "0", "COM3-Parity": "None", "405 AO-Volts": "0.0000", "PRIME-TransposeMirrorY": "0", "PRIME-ChipName": "GS144BSI", "470 AO-Volts": "0.0000", "PRIME-TransposeMirrorX": "0", "445 Shutter-SupportsTriggering": "0", "470 Shutter-SupportsTriggering": "0", "XLIGHT Hub-Description": "XLIGHT hub", "PRIME-PreampOffLimit": "10000", "470 AO-Sequenceable": "1", "528 Shutter-MaxVolts": "5.0000", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "Core-XYStage": "XYStage", "470 AO-TriggerSampleRate": "100000", "PRIME-Timing-PreTriggerDelayNs": "24672000.0000", "528-DA Device": "528 Shutter", "Focus-Description": "Nikon TE2000 focus adapter", "FilterBlock-State": "5", "520 AO-Demo": "No", "COM3-DelayBetweenCharsMs": "0.0000", "470 Shutter-Volts": "0.0000", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "PRIME-PP 2 THRESHOLD": "125", "PRIME-PP 4 THRESHOLD": "75", "XYStage-Acceleration": "4.0000", "470 Shutter-Demo": "No", "PRIME-Name": "PMPCIECam00", "640-DA Device": "640 Shutter", "405 AO-Block voltage": "false", "ElapsedTime-ms": 1207883, "PRIME-Offset": "100", "445 AO-IOChannel": "Dev1/ao1", "640 AO-MaxVolts": "5.0000", "PositionIndex": 29, "PRIME-PP 1 ENABLED": "Yes", "COM1-BaudRate": "9600", "640-State": "1", "COM3-Verbose": "1", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "LudlController-TransmissionDelay": "0", "PRIME-FullWellCapacity": "62000", "PRIME-SMARTStreamingEnabled": "No", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "640 Shutter-Description": "NI DAC", "528-Description": "DA device that is used as a shutter", "COM4-Description": "Serial port driver (boost:asio)", "405 Shutter-Description": "NI DAC", "TE2000-Name": "TE2000", "528 Shutter-Description": "NI DAC", "COM9-Description": "Serial port driver (boost:asio)", "LudlController-Name": "LudlController", "445 Shutter-TriggerSequenceLength": "1000", "PRIME-SerialNumber": "A18B203008", "445 AO-Volts": "0.0000", "PRIME-X-dimension": "1200", "PRIME-ShutterCloseDelay": "0", "XLIGHT Disk slider-State": "2", "Position": "Default", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 AO-IOChannel": "Dev1/ao5", "520 AO-IOChannel": "Dev1/ao4", "528 AO-TriggerSequenceLength": "1000", "405 Shutter-MaxVolts": "5.0000", "470-DA Device": "470 Shutter", "PRIME-FTCapable": "No", "Core-ChannelGroup": "Channels", "555 AO-SupportsTriggering": "0", "PRIME-Y-dimension": "1200", "Frame": 0, "555 Shutter-Sequenceable": "1", "PRIME-Timing-ExposureTimeNs": "499998000.0000", "Core-ImageProcessor": "", "528-State": "0", "520 AO-Block voltage": "false", "XLIGHT Excitation Wheel-Label": "QUAD", "640 Shutter-Demo": "No", "COM3-StopBits": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "520 Shutter-IOChannel": "Dev1/ao11", "Width": 1200, "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-SupportsTriggering": "0", "640 AO-TriggerSampleRate": "100000", "520-Description": "DA device that is used as a shutter", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "405 Shutter-Sequenceable": "1", "405 Shutter-SupportsTriggering": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-TriggerSampleRate": "100000", "640 Shutter-MaxVolts": "5.0000", "555 AO-MaxVolts": "5.0000", "520 AO-TriggerSequenceLength": "1000", "COM3-BaudRate": "9600", "COM9-BaudRate": "9600", "Vincent-D3-Channel #": "Ch. #1", "PRIME-PMode": "Normal", "OpticalPath-Label": "Left(100)", "BitDepth": 16, "555 AO-TriggerSequenceLength": "1000", "445 Shutter-Description": "NI DAC", "405 AO-Sequenceable": "1", "PRIME-AcquisitionMethod": "Callbacks", "555 AO-Name": "AnalogIO", "PRIME-ShutterMode": "Pre-Exposure", "COM3-Handshaking": "Off", "445 AO-SupportsTriggering": "0", "ROI": "0-0-1200-1200", "COM9-Verbose": "1", "PRIME-Color": "OFF", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "PRIME-ShutterOpenDelay": "0", "COM1-Parity": "None", "Slice": 9, "SlicePosition": 40, "COM9-DelayBetweenCharsMs": "0.0000", "555-Name": "DA Shutter", "PIEZO-LudlSingleAxisName": "B", "XLIGHT Emission Wheel-Label": "679/29 (A)", "640 Shutter-Name": "AnalogIO", "640 AO-Sequenceable": "1", "520 Shutter-Demo": "No", "528 Shutter-Demo": "No", "470-Name": "DA Shutter", "520-DA Device": "520 Shutter", "XYStage-Description": "Ludl XY stage driver adapter", "445 Shutter-MinVolts": "0.0000", "528 AO-TriggerSampleRate": "100000", "405 AO-MinVolts": "0.0000", "405 Shutter-TriggerSequenceLength": "1000", "Height": 1200, "555 AO-Block voltage": "false", "640 Shutter-Volts": "5.0000", "PixelType": "GRAY16", "445 Shutter-Block voltage": "false", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "520 Shutter-TriggerSequenceLength": "1000", "COM9-Name": "COM9", "Core-SLM": "", "555 AO-Volts": "0.0000", "555 Shutter-Block voltage": "false", "XYStage-Speed": "1800.0000", "520 AO-MaxVolts": "5.0000", "528 Shutter-SupportsTriggering": "0", "COM1-Description": "Serial port driver (boost:asio)", "PRIME-MetadataEnabled": "No", "520 Shutter-MinVolts": "0.0000", "470 Shutter-Sequenceable": "1", "PRIME-PreampDelay": "0", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-MinVolts": "0.0000", "ChannelIndex": 2, "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Core-AutoShutter": "1", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "Exposure-ms": 320, "PRIME-PP 2 ENABLED": "Yes", "640 Shutter-TriggerSampleRate": "100000", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "470 Shutter-IOChannel": "Dev1/ao10", "405 AO-Demo": "No", "PixelSizeUm": 1.1, "470 AO-Demo": "No", "470 Shutter-MaxVolts": "5.0000", "Vincent-D3-Name": "Vincent-D3", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FileName": "img_000000000_Il-A_009.tif", "LudlController-Reset": "Operate", "PRIME-Exposure": "320.00", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "445-Name": "DA Shutter", "555 Shutter-Name": "AnalogIO", "520 Shutter-TriggerSampleRate": "100000", "445 Shutter-IOChannel": "Dev1/ao9", "PRIME-TransposeCorrection": "0", "XYStage-StepSize": "0.1000", "445-Description": "DA device that is used as a shutter", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 AO-IOChannel": "Dev1/ao7", "520 Shutter-Name": "AnalogIO", "COM3-DataBits": "8", "640 AO-Volts": "4.5000", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "555 Shutter-IOChannel": "Dev1/ao13", "WaitInterval": null, "520 Shutter-Sequenceable": "1", "YPositionUm": -180, "445-State": "0", "445-DA Device": "445 Shutter", "PRIME-CircularBufferAutoSize": "ON", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "555 AO-TriggerSampleRate": "100000", "445 Shutter-Volts": "0.0000", "XLIGHT Hub-Port": "COM3", "Core-AutoFocus": "", "COM4-Handshaking": "Off", "528 Shutter-MinVolts": "0.0000", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "445 Shutter-Name": "AnalogIO", "528 Shutter-Sequenceable": "1", "XYStage-TransposeMirrorX": "0", "XYStage-TransposeMirrorY": "0", "PRIME-ReadoutRate": "100MHz 16bit", "XYStage-Name": "Stage", "640 AO-SupportsTriggering": "0", "640-Name": "DA Shutter", "640 Shutter-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "640 AO-Block voltage": "false", "445 Shutter-Sequenceable": "1", "470 AO-MaxVolts": "5.0000", "COM4-BaudRate": "9600", "470-Description": "DA device that is used as a shutter", "520 Shutter-Description": "NI DAC", "PRIME-CCDTemperature": "-14.8500", "Time": "2019-12-18 23:11:08 -0500", "640 AO-TriggerSequenceLength": "1000", "470 AO-Name": "AnalogIO", "XLIGHT Disk slider-Label": "Disk Pos 40um", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "Binning": "1x1", "TE2000-ModelType": "1", "COM3-AnswerTimeout": "500.0000", "555 Shutter-SupportsTriggering": "0", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "TE2000-Port": "COM4", "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Description": "NI DAC", "COM4-AnswerTimeout": "500.0000", "COM1-DelayBetweenCharsMs": "0.0000", "FilterBlock-Label": "Confocal", "445 AO-Name": "AnalogIO", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-Timing-ReadoutTimeNs": "24672000.0000", "528 AO-Demo": "No", "520 AO-Sequenceable": "1", "555-Description": "DA device that is used as a shutter", "405-DA Device": "405 Shutter", "405 AO-TriggerSequenceLength": "1000", "640 AO-MinVolts": "0.0000", "640 Shutter-Block voltage": "false", "FrameIndex": 0, "PRIME-Port": "CMOS", "555 AO-MinVolts": "0.0000", "COM3-Description": "Serial port driver (boost:asio)", "528 AO-Description": "NI DAC", "555 AO-IOChannel": "Dev1/ao6", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "555 AO-TriggerInputLine": "/Dev1/PFI0", "PRIME-ActualInterval-ms": "390.5200", "LudlController-Port": "COM9", "445 Shutter-Demo": "No", "PRIME-Trigger-Expose Out-Mux": "1", "SliceIndex": 9, "PRIME-CCDTemperatureSetPoint": "-15.0000", "405 Shutter-MinVolts": "0.0000", "PRIME-ExposeOutMode": "First Row", "AxisPositions": null, "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Volts": "0.0000", "528-Name": "DA Shutter", "555-State": "0", "XLIGHT Dichroic-Label": "Di03-R635-t1", "445 AO-Sequenceable": "1", "405-Name": "DA Shutter", "405 AO-IOChannel": "Dev1/ao0", "Core-Camera": "PRIME", "Focus-Name": "Focus", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "405 Shutter-IOChannel": "Dev1/ao8", "640 AO-Demo": "No", "640 Shutter-SupportsTriggering": "0", "445 AO-Demo": "No", "555-DA Device": "555 Shutter", "470 Shutter-Description": "NI DAC", "528 AO-Volts": "0.0000", "COM1-DataBits": "8", "Vincent-D3-Address": "x", "520 AO-SupportsTriggering": "0", "405-Description": "DA device that is used as a shutter", "640 Shutter-MinVolts": "0.0000", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "405 AO-Name": "AnalogIO", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "640 AO-TriggerInputLine": "/Dev1/PFI0", "405 AO-TriggerInputLine": "/Dev1/PFI0", "640-Description": "DA device that is used as a shutter", "445 Shutter-TriggerSampleRate": "100000", "470 Shutter-TriggerSequenceLength": "1000", "COM1-StopBits": "1", "520 Shutter-SupportsTriggering": "0", "405-State": "0", "Core-Initialize": "1", "XLIGHT Emission Wheel-State": "3", "520 AO-Description": "NI DAC", "528 AO-TriggerInputLine": "/Dev1/PFI0", "445 Shutter-MaxVolts": "5.0000", "COM9-Handshaking": "Off", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "XLIGHT Spin motor-Label": "On (spinning)", "PRIME-PP 5 ENABLED": "No", "UUID": "03ed404f-59d5-4874-8b33-e0e997dc470d", "PRIME-TriggerMode": "Internal Trigger", "COM1-Handshaking": "Off", "PRIME-Timing-PostTriggerDelayNs": "49344000.0000", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "Focus-Position": "17741.5000", "528 Shutter-Block voltage": "false", "Channel": "Il-A", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "555 Shutter-Demo": "No", "405 Shutter-Demo": "No", "528 AO-Name": "AnalogIO", "520-State": "0", "Core-Galvo": "", "405 Shutter-TriggerSampleRate": "100000", "Focus-FocusStepSize_(nm)": "100", "PRIME-PixelType": "16bit", "COM4-DataBits": "8", "470 AO-IOChannel": "Dev1/ao2", "520 AO-MinVolts": "0.0000", "COM9-Parity": "None", "PRIME-CircularBufferEnabled": "ON", "PRIME-PP 6 Reset": "No", "640 AO-Description": "NI DAC", "Source": "PRIME", "445 AO-MinVolts": "0.0000", "PIEZO-Name": "Stage", "445 AO-Description": "NI DAC", "PRIME-CircularBufferFrameCount": "46", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "520 AO-Volts": "0.0000", "528 Shutter-TriggerSampleRate": "100000", "XPositionUm": 15712.1, "OpticalPath-State": "4", "PRIME-PP 3 THRESHOLD": "80", "PRIME-PVCAM Version": "3.7.1", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Gain": "1-HDR", "640 Shutter-TriggerSequenceLength": "1000", "528 AO-SupportsTriggering": "0", "445 AO-TriggerInputLine": "/Dev1/PFI0", "OpticalPath-Name": "OpticalPath", "PRIME-PP 4 ENABLED": "Yes", "PRIME-PVCAM Adapter Version": "1.3.29", "COM3-Name": "COM3", "405 AO-MaxVolts": "5.0000", "520 Shutter-Block voltage": "false", "PRIME-Timing-ClearingTimeNs": "49344000.0000", "Core-Focus": "PIEZO", "528 AO-Block voltage": "false", "TE2000-Description": "Nikon TE2000 hub", "528 AO-MinVolts": "0.0000", "COM4-Parity": "None", "Summary": { "Slices": 11, "ChContrastMin": [ 0, 0, 0, 0, 0, 0 ], "PixelAspect": 1, "SlicesFirst": true, "Interval_ms": 0, "Time": "2019-12-18 23:10:50 -0500", "Prefix": "seq_5", "InitialPositionList": [ { "Label": "Pos0", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.21], "Focus": [17741.5], "XYStage": [ 44542.200000000004, 31636.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos1", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.22], "Focus": [17741.4], "XYStage": [ 43801.100000000006, 31637.9 ] }, "GridColumnIndex": 0 }, { "Label": "Pos2", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.5], "XYStage": [ 38561.3, 32040.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos3", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.185], "Focus": [17741.3], "XYStage": [ 37698.4, 32041.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos4", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.16], "Focus": [17741.5], "XYStage": [ 32375.4, 31758.9 ] }, "GridColumnIndex": 0 }, { "Label": "Pos5", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.17000000000002], "Focus": [17741.3], "XYStage": [ 31770.7, 31758.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos6", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.4], "XYStage": [ 26536.800000000003, 31068.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos7", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.205], "Focus": [17741.4], "XYStage": [ 25420.2, 31068.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos8", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.85], "Focus": [17741.3], "XYStage": [ 21059, 31508 ] }, "GridColumnIndex": 0 }, { "Label": "Pos9", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.82], "Focus": [17741.3], "XYStage": [ 19731.9, 31506.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos10", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.79], "Focus": [17741.4], "XYStage": [ 14528.5, 32007.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos11", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.83], "Focus": [17741.2], "XYStage": [ 13830.300000000001, 32009.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos12", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.79], "Focus": [17741.3], "XYStage": [ 8775.300000000001, 32017.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos13", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.815], "Focus": [17741.3], "XYStage": [ 8109.200000000001, 32008.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos14", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.805], "Focus": [17741.3], "XYStage": [ 3483.3, 31908.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos15", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.82], "Focus": [17741.2], "XYStage": [ 2686.8, 31903.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos16", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [211.48000000000002], "Focus": [17741.1], "XYStage": [ -1978.9, 31973.600000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos17", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [211.51500000000001], "Focus": [17741.1], "XYStage": [ -2957.8, 31982.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos18", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.195], "Focus": [17741.5], "XYStage": [ 47747.4, -576.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos19", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.23000000000002], "Focus": [17741.6], "XYStage": [ 47053.9, -576.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos20", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.205], "Focus": [17741.4], "XYStage": [ 41871.200000000004, -479.8 ] }, "GridColumnIndex": 0 }, { "Label": "Pos21", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.4], "XYStage": [ 40882.600000000006, -392 ] }, "GridColumnIndex": 0 }, { "Label": "Pos22", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.24], "Focus": [17741.4], "XYStage": [ 35905.6, -135.3 ] }, "GridColumnIndex": 0 }, { "Label": "Pos23", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.195], "Focus": [17741.5], "XYStage": [ 34990.9, -132.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos24", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.46], "Focus": [17741.4], "XYStage": [ 29678.100000000002, -958.1 ] }, "GridColumnIndex": 0 }, { "Label": "Pos25", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.44], "Focus": [17741.5], "XYStage": [ 28983.600000000002, -939.4000000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos26", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.475], "Focus": [17741.5], "XYStage": [ 23019.7, -122 ] }, "GridColumnIndex": 0 }, { "Label": "Pos27", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.44], "Focus": [17741.7], "XYStage": [ 22428, -116.80000000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos28", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.46], "Focus": [17741.7], "XYStage": [ 16364.900000000001, -181.10000000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos29", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.48000000000002], "Focus": [17741.5], "XYStage": [ 15712.1, -180 ] }, "GridColumnIndex": 0 }, { "Label": "Pos30", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.45000000000002], "Focus": [17741.7], "XYStage": [ 10346.1, 77.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos31", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.49], "Focus": [17741.4], "XYStage": [ 9755.5, 85.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos32", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.475], "Focus": [17741.5], "XYStage": [ 4775.5, 293.90000000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos33", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.445], "Focus": [17741.4], "XYStage": [ 3954.9, 293.90000000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos34", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.455], "Focus": [17741.4], "XYStage": [ -1324.9, 545.6 ] }, "GridColumnIndex": 0 }, { "Label": "Pos35", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.445], "Focus": [17741.3], "XYStage": [ -2097, 576.6 ] }, "GridColumnIndex": 0 } ], "z-step_um": 10, "GridRow": 0, "BitDepth": 16, "Depth": 2, "Channels": 6, "Source": "Micro-Manager", "ChColors": [ -16711681, -256, -65281, -1, -1, -16776961 ], "MetadataVersion": 10, "KeepShutterOpenChannels": true, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536, 65536 ], "CustomIntervals_ms": [], "Height": 1200, "MicroManagerVersion": "1.4.23 20180219", "GridColumn": 0, "UUID": "67598d21-5db5-4842-ab60-f506f7b42c7c", "PixelType": "GRAY16", "Width": 1200, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\Yan\\20191218-YC113-S7S8-miseq", "Frames": 1, "Comment": "", "UserName": "Nikon", "PixelSize_um": 1.1, "ROI": [ 0, 0, 1200, 1200 ], "Date": "2019-12-18", "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC", "P-DAPI-high" ], "KeepShutterOpenSlices": true, "IJType": 1, "ComputerName": "ZadorConfocal2", "Positions": 36, "PositionIndex": 29, "CameraTimeout": 20000, "TimeFirst": false }, "640 AO-Name": "AnalogIO", "Vincent-D3-Command": "Close" }, "FrameKey-0-2-10": { "COM4-StopBits": "1", "528 AO-MaxVolts": "5.0000", "XLIGHT Excitation Wheel-State": "1", "470 Shutter-Name": "AnalogIO", "Core-Shutter": "640", "555 AO-Demo": "No", "XLIGHT Dichroic-State": "2", "XYStage-StepSize-Y": "0.1000", "ZPositionUm": 268.48, "XYStage-StepSize-X": "0.1000", "PRIME-FirmwareVersion": "20.28", "PRIME-ClearCycles": "2", "520-Name": "DA Shutter", "405 Shutter-Name": "AnalogIO", "PIEZO-StepSize": "0.0050", "PRIME-ClearMode": "Pre-Exposure", "470 AO-MinVolts": "0.0000", "405 AO-SupportsTriggering": "1", "CameraChannelIndex": 0, "XYStage-StartSpeed": "500.0000", "COM9-StopBits": "2", "405 AO-Description": "NI DAC", "COM4-DelayBetweenCharsMs": "0.0000", "445 AO-Block voltage": "false", "520 AO-TriggerInputLine": "/Dev1/PFI0", "555 AO-Description": "NI DAC", "520 AO-TriggerSampleRate": "100000", "COM4-Verbose": "1", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM4-Name": "COM4", "405 Shutter-Volts": "0.0000", "Vincent-D3-Port": "COM1", "PRIME-PP 4 MIN ADU AFFECTED": "200", "470 Shutter-MinVolts": "0.0000", "PRIME-PP 3 ENABLED": "Yes", "470 AO-Description": "NI DAC", "PRIME-Description": "PVCAM API device adapter", "520 Shutter-MaxVolts": "5.0000", "520 Shutter-Volts": "0.0000", "528 AO-Sequenceable": "1", "PRIME-Binning": "1x1", "555 Shutter-MaxVolts": "5.0000", "528 Shutter-TriggerSequenceLength": "1000", "405 Shutter-Block voltage": "false", "XLIGHT Spin motor-State": "1", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "Vincent-D3-Time to open (ms)": "35.0000", "Vincent-D3-Time to close (ms)": "35.0000", "445 AO-TriggerSampleRate": "100000", "640 Shutter-IOChannel": "Dev1/ao14", "555 AO-Sequenceable": "1", "520 AO-Name": "AnalogIO", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "470 AO-Block voltage": "false", "COM1-Verbose": "1", "COM9-AnswerTimeout": "10000.0000", "528 Shutter-Name": "AnalogIO", "PRIME-Trigger Timeout (secs)": "10", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "NextFrame": 0, "470 AO-TriggerSequenceLength": "1000", "PRIME-TransposeXY": "0", "445 AO-TriggerSequenceLength": "1000", "COM1-Name": "COM1", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "COM9-DataBits": "8", "COM1-AnswerTimeout": "500.0000", "Camera": "", "TE2000-Version": "V2.50-V2.50-V2.10", "528 Shutter-Volts": "0.0000", "XLIGHT Hub-Name": "XLIGHT Hub", "PIEZO-Description": "Ludl stage driver adapter", "PositionName": "Pos29", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSampleRate": "100000", "PRIME-FanSpeedSetpoint": "High", "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "405 AO-TriggerSampleRate": "100000", "470 Shutter-Block voltage": "false", "470-State": "0", "COM3-Parity": "None", "405 AO-Volts": "0.0000", "PRIME-TransposeMirrorY": "0", "PRIME-ChipName": "GS144BSI", "470 AO-Volts": "0.0000", "PRIME-TransposeMirrorX": "0", "445 Shutter-SupportsTriggering": "0", "470 Shutter-SupportsTriggering": "0", "XLIGHT Hub-Description": "XLIGHT hub", "PRIME-PreampOffLimit": "10000", "470 AO-Sequenceable": "1", "528 Shutter-MaxVolts": "5.0000", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "Core-XYStage": "XYStage", "470 AO-TriggerSampleRate": "100000", "PRIME-Timing-PreTriggerDelayNs": "24672000.0000", "528-DA Device": "528 Shutter", "Focus-Description": "Nikon TE2000 focus adapter", "FilterBlock-State": "5", "520 AO-Demo": "No", "COM3-DelayBetweenCharsMs": "0.0000", "470 Shutter-Volts": "0.0000", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "PRIME-PP 2 THRESHOLD": "125", "PRIME-PP 4 THRESHOLD": "75", "XYStage-Acceleration": "4.0000", "470 Shutter-Demo": "No", "PRIME-Name": "PMPCIECam00", "640-DA Device": "640 Shutter", "405 AO-Block voltage": "false", "ElapsedTime-ms": 1208441, "PRIME-Offset": "100", "445 AO-IOChannel": "Dev1/ao1", "640 AO-MaxVolts": "5.0000", "PositionIndex": 29, "PRIME-PP 1 ENABLED": "Yes", "COM1-BaudRate": "9600", "640-State": "1", "COM3-Verbose": "1", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "LudlController-TransmissionDelay": "0", "PRIME-FullWellCapacity": "62000", "PRIME-SMARTStreamingEnabled": "No", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "640 Shutter-Description": "NI DAC", "528-Description": "DA device that is used as a shutter", "COM4-Description": "Serial port driver (boost:asio)", "405 Shutter-Description": "NI DAC", "TE2000-Name": "TE2000", "528 Shutter-Description": "NI DAC", "COM9-Description": "Serial port driver (boost:asio)", "LudlController-Name": "LudlController", "445 Shutter-TriggerSequenceLength": "1000", "PRIME-SerialNumber": "A18B203008", "445 AO-Volts": "0.0000", "PRIME-X-dimension": "1200", "PRIME-ShutterCloseDelay": "0", "XLIGHT Disk slider-State": "2", "Position": "Default", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 AO-IOChannel": "Dev1/ao5", "520 AO-IOChannel": "Dev1/ao4", "528 AO-TriggerSequenceLength": "1000", "405 Shutter-MaxVolts": "5.0000", "470-DA Device": "470 Shutter", "PRIME-FTCapable": "No", "Core-ChannelGroup": "Channels", "555 AO-SupportsTriggering": "0", "PRIME-Y-dimension": "1200", "Frame": 0, "555 Shutter-Sequenceable": "1", "PRIME-Timing-ExposureTimeNs": "499998000.0000", "Core-ImageProcessor": "", "528-State": "0", "520 AO-Block voltage": "false", "XLIGHT Excitation Wheel-Label": "QUAD", "640 Shutter-Demo": "No", "COM3-StopBits": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "520 Shutter-IOChannel": "Dev1/ao11", "Width": 1200, "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-SupportsTriggering": "0", "640 AO-TriggerSampleRate": "100000", "520-Description": "DA device that is used as a shutter", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "405 Shutter-Sequenceable": "1", "405 Shutter-SupportsTriggering": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-TriggerSampleRate": "100000", "640 Shutter-MaxVolts": "5.0000", "555 AO-MaxVolts": "5.0000", "520 AO-TriggerSequenceLength": "1000", "COM3-BaudRate": "9600", "COM9-BaudRate": "9600", "Vincent-D3-Channel #": "Ch. #1", "PRIME-PMode": "Normal", "OpticalPath-Label": "Left(100)", "BitDepth": 16, "555 AO-TriggerSequenceLength": "1000", "445 Shutter-Description": "NI DAC", "405 AO-Sequenceable": "1", "PRIME-AcquisitionMethod": "Callbacks", "555 AO-Name": "AnalogIO", "PRIME-ShutterMode": "Pre-Exposure", "COM3-Handshaking": "Off", "445 AO-SupportsTriggering": "0", "ROI": "0-0-1200-1200", "COM9-Verbose": "1", "PRIME-Color": "OFF", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "PRIME-ShutterOpenDelay": "0", "COM1-Parity": "None", "Slice": 10, "SlicePosition": 50, "COM9-DelayBetweenCharsMs": "0.0000", "555-Name": "DA Shutter", "PIEZO-LudlSingleAxisName": "B", "XLIGHT Emission Wheel-Label": "679/29 (A)", "640 Shutter-Name": "AnalogIO", "640 AO-Sequenceable": "1", "520 Shutter-Demo": "No", "528 Shutter-Demo": "No", "470-Name": "DA Shutter", "520-DA Device": "520 Shutter", "XYStage-Description": "Ludl XY stage driver adapter", "445 Shutter-MinVolts": "0.0000", "528 AO-TriggerSampleRate": "100000", "405 AO-MinVolts": "0.0000", "405 Shutter-TriggerSequenceLength": "1000", "Height": 1200, "555 AO-Block voltage": "false", "640 Shutter-Volts": "5.0000", "PixelType": "GRAY16", "445 Shutter-Block voltage": "false", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "520 Shutter-TriggerSequenceLength": "1000", "COM9-Name": "COM9", "Core-SLM": "", "555 AO-Volts": "0.0000", "555 Shutter-Block voltage": "false", "XYStage-Speed": "1800.0000", "520 AO-MaxVolts": "5.0000", "528 Shutter-SupportsTriggering": "0", "COM1-Description": "Serial port driver (boost:asio)", "PRIME-MetadataEnabled": "No", "520 Shutter-MinVolts": "0.0000", "470 Shutter-Sequenceable": "1", "PRIME-PreampDelay": "0", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-MinVolts": "0.0000", "ChannelIndex": 2, "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Core-AutoShutter": "1", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "Exposure-ms": 320, "PRIME-PP 2 ENABLED": "Yes", "640 Shutter-TriggerSampleRate": "100000", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "470 Shutter-IOChannel": "Dev1/ao10", "405 AO-Demo": "No", "PixelSizeUm": 1.1, "470 AO-Demo": "No", "470 Shutter-MaxVolts": "5.0000", "Vincent-D3-Name": "Vincent-D3", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FileName": "img_000000000_Il-A_010.tif", "LudlController-Reset": "Operate", "PRIME-Exposure": "320.00", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "445-Name": "DA Shutter", "555 Shutter-Name": "AnalogIO", "520 Shutter-TriggerSampleRate": "100000", "445 Shutter-IOChannel": "Dev1/ao9", "PRIME-TransposeCorrection": "0", "XYStage-StepSize": "0.1000", "445-Description": "DA device that is used as a shutter", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 AO-IOChannel": "Dev1/ao7", "520 Shutter-Name": "AnalogIO", "COM3-DataBits": "8", "640 AO-Volts": "4.5000", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "555 Shutter-IOChannel": "Dev1/ao13", "WaitInterval": null, "520 Shutter-Sequenceable": "1", "YPositionUm": -180, "445-State": "0", "445-DA Device": "445 Shutter", "PRIME-CircularBufferAutoSize": "ON", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "555 AO-TriggerSampleRate": "100000", "445 Shutter-Volts": "0.0000", "XLIGHT Hub-Port": "COM3", "Core-AutoFocus": "", "COM4-Handshaking": "Off", "528 Shutter-MinVolts": "0.0000", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "445 Shutter-Name": "AnalogIO", "528 Shutter-Sequenceable": "1", "XYStage-TransposeMirrorX": "0", "XYStage-TransposeMirrorY": "0", "PRIME-ReadoutRate": "100MHz 16bit", "XYStage-Name": "Stage", "640 AO-SupportsTriggering": "0", "640-Name": "DA Shutter", "640 Shutter-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "640 AO-Block voltage": "false", "445 Shutter-Sequenceable": "1", "470 AO-MaxVolts": "5.0000", "COM4-BaudRate": "9600", "470-Description": "DA device that is used as a shutter", "520 Shutter-Description": "NI DAC", "PRIME-CCDTemperature": "-14.8500", "Time": "2019-12-18 23:11:08 -0500", "640 AO-TriggerSequenceLength": "1000", "470 AO-Name": "AnalogIO", "XLIGHT Disk slider-Label": "Disk Pos 40um", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "Binning": "1x1", "TE2000-ModelType": "1", "COM3-AnswerTimeout": "500.0000", "555 Shutter-SupportsTriggering": "0", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "TE2000-Port": "COM4", "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Description": "NI DAC", "COM4-AnswerTimeout": "500.0000", "COM1-DelayBetweenCharsMs": "0.0000", "FilterBlock-Label": "Confocal", "445 AO-Name": "AnalogIO", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-Timing-ReadoutTimeNs": "24672000.0000", "528 AO-Demo": "No", "520 AO-Sequenceable": "1", "555-Description": "DA device that is used as a shutter", "405-DA Device": "405 Shutter", "405 AO-TriggerSequenceLength": "1000", "640 AO-MinVolts": "0.0000", "640 Shutter-Block voltage": "false", "FrameIndex": 0, "PRIME-Port": "CMOS", "555 AO-MinVolts": "0.0000", "COM3-Description": "Serial port driver (boost:asio)", "528 AO-Description": "NI DAC", "555 AO-IOChannel": "Dev1/ao6", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "555 AO-TriggerInputLine": "/Dev1/PFI0", "PRIME-ActualInterval-ms": "390.5200", "LudlController-Port": "COM9", "445 Shutter-Demo": "No", "PRIME-Trigger-Expose Out-Mux": "1", "SliceIndex": 10, "PRIME-CCDTemperatureSetPoint": "-15.0000", "405 Shutter-MinVolts": "0.0000", "PRIME-ExposeOutMode": "First Row", "AxisPositions": null, "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Volts": "0.0000", "528-Name": "DA Shutter", "555-State": "0", "XLIGHT Dichroic-Label": "Di03-R635-t1", "445 AO-Sequenceable": "1", "405-Name": "DA Shutter", "405 AO-IOChannel": "Dev1/ao0", "Core-Camera": "PRIME", "Focus-Name": "Focus", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "405 Shutter-IOChannel": "Dev1/ao8", "640 AO-Demo": "No", "640 Shutter-SupportsTriggering": "0", "445 AO-Demo": "No", "555-DA Device": "555 Shutter", "470 Shutter-Description": "NI DAC", "528 AO-Volts": "0.0000", "COM1-DataBits": "8", "Vincent-D3-Address": "x", "520 AO-SupportsTriggering": "0", "405-Description": "DA device that is used as a shutter", "640 Shutter-MinVolts": "0.0000", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "405 AO-Name": "AnalogIO", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "640 AO-TriggerInputLine": "/Dev1/PFI0", "405 AO-TriggerInputLine": "/Dev1/PFI0", "640-Description": "DA device that is used as a shutter", "445 Shutter-TriggerSampleRate": "100000", "470 Shutter-TriggerSequenceLength": "1000", "COM1-StopBits": "1", "520 Shutter-SupportsTriggering": "0", "405-State": "0", "Core-Initialize": "1", "XLIGHT Emission Wheel-State": "3", "520 AO-Description": "NI DAC", "528 AO-TriggerInputLine": "/Dev1/PFI0", "445 Shutter-MaxVolts": "5.0000", "COM9-Handshaking": "Off", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "XLIGHT Spin motor-Label": "On (spinning)", "PRIME-PP 5 ENABLED": "No", "UUID": "9b659c1c-57dd-454b-a440-3ba21bca4fc8", "PRIME-TriggerMode": "Internal Trigger", "COM1-Handshaking": "Off", "PRIME-Timing-PostTriggerDelayNs": "49344000.0000", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "Focus-Position": "17741.5000", "528 Shutter-Block voltage": "false", "Channel": "Il-A", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "555 Shutter-Demo": "No", "405 Shutter-Demo": "No", "528 AO-Name": "AnalogIO", "520-State": "0", "Core-Galvo": "", "405 Shutter-TriggerSampleRate": "100000", "Focus-FocusStepSize_(nm)": "100", "PRIME-PixelType": "16bit", "COM4-DataBits": "8", "470 AO-IOChannel": "Dev1/ao2", "520 AO-MinVolts": "0.0000", "COM9-Parity": "None", "PRIME-CircularBufferEnabled": "ON", "PRIME-PP 6 Reset": "No", "640 AO-Description": "NI DAC", "Source": "PRIME", "445 AO-MinVolts": "0.0000", "PIEZO-Name": "Stage", "445 AO-Description": "NI DAC", "PRIME-CircularBufferFrameCount": "46", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "520 AO-Volts": "0.0000", "528 Shutter-TriggerSampleRate": "100000", "XPositionUm": 15712.1, "OpticalPath-State": "4", "PRIME-PP 3 THRESHOLD": "80", "PRIME-PVCAM Version": "3.7.1", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Gain": "1-HDR", "640 Shutter-TriggerSequenceLength": "1000", "528 AO-SupportsTriggering": "0", "445 AO-TriggerInputLine": "/Dev1/PFI0", "OpticalPath-Name": "OpticalPath", "PRIME-PP 4 ENABLED": "Yes", "PRIME-PVCAM Adapter Version": "1.3.29", "COM3-Name": "COM3", "405 AO-MaxVolts": "5.0000", "520 Shutter-Block voltage": "false", "PRIME-Timing-ClearingTimeNs": "49344000.0000", "Core-Focus": "PIEZO", "528 AO-Block voltage": "false", "TE2000-Description": "Nikon TE2000 hub", "528 AO-MinVolts": "0.0000", "COM4-Parity": "None", "Summary": { "Slices": 11, "ChContrastMin": [ 0, 0, 0, 0, 0, 0 ], "PixelAspect": 1, "SlicesFirst": true, "Interval_ms": 0, "Time": "2019-12-18 23:10:50 -0500", "Prefix": "seq_5", "InitialPositionList": [ { "Label": "Pos0", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.21], "Focus": [17741.5], "XYStage": [ 44542.200000000004, 31636.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos1", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.22], "Focus": [17741.4], "XYStage": [ 43801.100000000006, 31637.9 ] }, "GridColumnIndex": 0 }, { "Label": "Pos2", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.5], "XYStage": [ 38561.3, 32040.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos3", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.185], "Focus": [17741.3], "XYStage": [ 37698.4, 32041.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos4", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.16], "Focus": [17741.5], "XYStage": [ 32375.4, 31758.9 ] }, "GridColumnIndex": 0 }, { "Label": "Pos5", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.17000000000002], "Focus": [17741.3], "XYStage": [ 31770.7, 31758.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos6", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.4], "XYStage": [ 26536.800000000003, 31068.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos7", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.205], "Focus": [17741.4], "XYStage": [ 25420.2, 31068.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos8", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.85], "Focus": [17741.3], "XYStage": [ 21059, 31508 ] }, "GridColumnIndex": 0 }, { "Label": "Pos9", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.82], "Focus": [17741.3], "XYStage": [ 19731.9, 31506.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos10", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.79], "Focus": [17741.4], "XYStage": [ 14528.5, 32007.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos11", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.83], "Focus": [17741.2], "XYStage": [ 13830.300000000001, 32009.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos12", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.79], "Focus": [17741.3], "XYStage": [ 8775.300000000001, 32017.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos13", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.815], "Focus": [17741.3], "XYStage": [ 8109.200000000001, 32008.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos14", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.805], "Focus": [17741.3], "XYStage": [ 3483.3, 31908.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos15", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.82], "Focus": [17741.2], "XYStage": [ 2686.8, 31903.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos16", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [211.48000000000002], "Focus": [17741.1], "XYStage": [ -1978.9, 31973.600000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos17", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [211.51500000000001], "Focus": [17741.1], "XYStage": [ -2957.8, 31982.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos18", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.195], "Focus": [17741.5], "XYStage": [ 47747.4, -576.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos19", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.23000000000002], "Focus": [17741.6], "XYStage": [ 47053.9, -576.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos20", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.205], "Focus": [17741.4], "XYStage": [ 41871.200000000004, -479.8 ] }, "GridColumnIndex": 0 }, { "Label": "Pos21", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.4], "XYStage": [ 40882.600000000006, -392 ] }, "GridColumnIndex": 0 }, { "Label": "Pos22", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.24], "Focus": [17741.4], "XYStage": [ 35905.6, -135.3 ] }, "GridColumnIndex": 0 }, { "Label": "Pos23", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.195], "Focus": [17741.5], "XYStage": [ 34990.9, -132.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos24", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.46], "Focus": [17741.4], "XYStage": [ 29678.100000000002, -958.1 ] }, "GridColumnIndex": 0 }, { "Label": "Pos25", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.44], "Focus": [17741.5], "XYStage": [ 28983.600000000002, -939.4000000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos26", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.475], "Focus": [17741.5], "XYStage": [ 23019.7, -122 ] }, "GridColumnIndex": 0 }, { "Label": "Pos27", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.44], "Focus": [17741.7], "XYStage": [ 22428, -116.80000000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos28", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.46], "Focus": [17741.7], "XYStage": [ 16364.900000000001, -181.10000000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos29", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.48000000000002], "Focus": [17741.5], "XYStage": [ 15712.1, -180 ] }, "GridColumnIndex": 0 }, { "Label": "Pos30", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.45000000000002], "Focus": [17741.7], "XYStage": [ 10346.1, 77.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos31", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.49], "Focus": [17741.4], "XYStage": [ 9755.5, 85.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos32", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.475], "Focus": [17741.5], "XYStage": [ 4775.5, 293.90000000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos33", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.445], "Focus": [17741.4], "XYStage": [ 3954.9, 293.90000000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos34", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.455], "Focus": [17741.4], "XYStage": [ -1324.9, 545.6 ] }, "GridColumnIndex": 0 }, { "Label": "Pos35", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.445], "Focus": [17741.3], "XYStage": [ -2097, 576.6 ] }, "GridColumnIndex": 0 } ], "z-step_um": 10, "GridRow": 0, "BitDepth": 16, "Depth": 2, "Channels": 6, "Source": "Micro-Manager", "ChColors": [ -16711681, -256, -65281, -1, -1, -16776961 ], "MetadataVersion": 10, "KeepShutterOpenChannels": true, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536, 65536 ], "CustomIntervals_ms": [], "Height": 1200, "MicroManagerVersion": "1.4.23 20180219", "GridColumn": 0, "UUID": "67598d21-5db5-4842-ab60-f506f7b42c7c", "PixelType": "GRAY16", "Width": 1200, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\Yan\\20191218-YC113-S7S8-miseq", "Frames": 1, "Comment": "", "UserName": "Nikon", "PixelSize_um": 1.1, "ROI": [ 0, 0, 1200, 1200 ], "Date": "2019-12-18", "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC", "P-DAPI-high" ], "KeepShutterOpenSlices": true, "IJType": 1, "ComputerName": "ZadorConfocal2", "Positions": 36, "PositionIndex": 29, "CameraTimeout": 20000, "TimeFirst": false }, "640 AO-Name": "AnalogIO", "Vincent-D3-Command": "Close" }, "FrameKey-0-3-0": { "COM4-StopBits": "1", "528 AO-MaxVolts": "5.0000", "XLIGHT Excitation Wheel-State": "1", "470 Shutter-Name": "AnalogIO", "Core-Shutter": "640", "555 AO-Demo": "No", "XLIGHT Dichroic-State": "2", "XYStage-StepSize-Y": "0.1000", "ZPositionUm": 168.48000000000002, "XYStage-StepSize-X": "0.1000", "PRIME-FirmwareVersion": "20.28", "PRIME-ClearCycles": "2", "520-Name": "DA Shutter", "405 Shutter-Name": "AnalogIO", "PIEZO-StepSize": "0.0050", "PRIME-ClearMode": "Pre-Exposure", "470 AO-MinVolts": "0.0000", "405 AO-SupportsTriggering": "1", "CameraChannelIndex": 0, "XYStage-StartSpeed": "500.0000", "COM9-StopBits": "2", "405 AO-Description": "NI DAC", "COM4-DelayBetweenCharsMs": "0.0000", "445 AO-Block voltage": "false", "520 AO-TriggerInputLine": "/Dev1/PFI0", "555 AO-Description": "NI DAC", "520 AO-TriggerSampleRate": "100000", "COM4-Verbose": "1", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM4-Name": "COM4", "405 Shutter-Volts": "0.0000", "Vincent-D3-Port": "COM1", "PRIME-PP 4 MIN ADU AFFECTED": "200", "470 Shutter-MinVolts": "0.0000", "PRIME-PP 3 ENABLED": "Yes", "470 AO-Description": "NI DAC", "PRIME-Description": "PVCAM API device adapter", "520 Shutter-MaxVolts": "5.0000", "520 Shutter-Volts": "0.0000", "528 AO-Sequenceable": "1", "PRIME-Binning": "1x1", "555 Shutter-MaxVolts": "5.0000", "528 Shutter-TriggerSequenceLength": "1000", "405 Shutter-Block voltage": "false", "XLIGHT Spin motor-State": "1", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "Vincent-D3-Time to open (ms)": "35.0000", "Vincent-D3-Time to close (ms)": "35.0000", "445 AO-TriggerSampleRate": "100000", "640 Shutter-IOChannel": "Dev1/ao14", "555 AO-Sequenceable": "1", "520 AO-Name": "AnalogIO", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "470 AO-Block voltage": "false", "COM1-Verbose": "1", "COM9-AnswerTimeout": "10000.0000", "528 Shutter-Name": "AnalogIO", "PRIME-Trigger Timeout (secs)": "10", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "NextFrame": 0, "470 AO-TriggerSequenceLength": "1000", "PRIME-TransposeXY": "0", "445 AO-TriggerSequenceLength": "1000", "COM1-Name": "COM1", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "COM9-DataBits": "8", "COM1-AnswerTimeout": "500.0000", "Camera": "", "TE2000-Version": "V2.50-V2.50-V2.10", "528 Shutter-Volts": "0.0000", "XLIGHT Hub-Name": "XLIGHT Hub", "PIEZO-Description": "Ludl stage driver adapter", "PositionName": "Pos29", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSampleRate": "100000", "PRIME-FanSpeedSetpoint": "High", "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "405 AO-TriggerSampleRate": "100000", "470 Shutter-Block voltage": "false", "470-State": "0", "COM3-Parity": "None", "405 AO-Volts": "0.0000", "PRIME-TransposeMirrorY": "0", "PRIME-ChipName": "GS144BSI", "470 AO-Volts": "0.0000", "PRIME-TransposeMirrorX": "0", "445 Shutter-SupportsTriggering": "0", "470 Shutter-SupportsTriggering": "0", "XLIGHT Hub-Description": "XLIGHT hub", "PRIME-PreampOffLimit": "10000", "470 AO-Sequenceable": "1", "528 Shutter-MaxVolts": "5.0000", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "Core-XYStage": "XYStage", "470 AO-TriggerSampleRate": "100000", "PRIME-Timing-PreTriggerDelayNs": "24672000.0000", "528-DA Device": "528 Shutter", "Focus-Description": "Nikon TE2000 focus adapter", "FilterBlock-State": "5", "520 AO-Demo": "No", "COM3-DelayBetweenCharsMs": "0.0000", "470 Shutter-Volts": "0.0000", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "PRIME-PP 2 THRESHOLD": "125", "PRIME-PP 4 THRESHOLD": "75", "XYStage-Acceleration": "4.0000", "470 Shutter-Demo": "No", "PRIME-Name": "PMPCIECam00", "640-DA Device": "640 Shutter", "405 AO-Block voltage": "false", "ElapsedTime-ms": 1209556, "PRIME-Offset": "100", "445 AO-IOChannel": "Dev1/ao1", "640 AO-MaxVolts": "5.0000", "PositionIndex": 29, "PRIME-PP 1 ENABLED": "Yes", "COM1-BaudRate": "9600", "640-State": "1", "COM3-Verbose": "1", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "LudlController-TransmissionDelay": "0", "PRIME-FullWellCapacity": "62000", "PRIME-SMARTStreamingEnabled": "No", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "640 Shutter-Description": "NI DAC", "528-Description": "DA device that is used as a shutter", "COM4-Description": "Serial port driver (boost:asio)", "405 Shutter-Description": "NI DAC", "TE2000-Name": "TE2000", "528 Shutter-Description": "NI DAC", "COM9-Description": "Serial port driver (boost:asio)", "LudlController-Name": "LudlController", "445 Shutter-TriggerSequenceLength": "1000", "PRIME-SerialNumber": "A18B203008", "445 AO-Volts": "0.0000", "PRIME-X-dimension": "1200", "PRIME-ShutterCloseDelay": "0", "XLIGHT Disk slider-State": "2", "Position": "Default", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 AO-IOChannel": "Dev1/ao5", "520 AO-IOChannel": "Dev1/ao4", "528 AO-TriggerSequenceLength": "1000", "405 Shutter-MaxVolts": "5.0000", "470-DA Device": "470 Shutter", "PRIME-FTCapable": "No", "Core-ChannelGroup": "Channels", "555 AO-SupportsTriggering": "0", "PRIME-Y-dimension": "1200", "Frame": 0, "555 Shutter-Sequenceable": "1", "PRIME-Timing-ExposureTimeNs": "499998000.0000", "Core-ImageProcessor": "", "528-State": "0", "520 AO-Block voltage": "false", "XLIGHT Excitation Wheel-Label": "QUAD", "640 Shutter-Demo": "No", "COM3-StopBits": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "520 Shutter-IOChannel": "Dev1/ao11", "Width": 1200, "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-SupportsTriggering": "0", "640 AO-TriggerSampleRate": "100000", "520-Description": "DA device that is used as a shutter", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "405 Shutter-Sequenceable": "1", "405 Shutter-SupportsTriggering": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-TriggerSampleRate": "100000", "640 Shutter-MaxVolts": "5.0000", "555 AO-MaxVolts": "5.0000", "520 AO-TriggerSequenceLength": "1000", "COM3-BaudRate": "9600", "COM9-BaudRate": "9600", "Vincent-D3-Channel #": "Ch. #1", "PRIME-PMode": "Normal", "OpticalPath-Label": "Left(100)", "BitDepth": 16, "555 AO-TriggerSequenceLength": "1000", "445 Shutter-Description": "NI DAC", "405 AO-Sequenceable": "1", "PRIME-AcquisitionMethod": "Callbacks", "555 AO-Name": "AnalogIO", "PRIME-ShutterMode": "Pre-Exposure", "COM3-Handshaking": "Off", "445 AO-SupportsTriggering": "0", "ROI": "0-0-1200-1200", "COM9-Verbose": "1", "PRIME-Color": "OFF", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "PRIME-ShutterOpenDelay": "0", "COM1-Parity": "None", "Slice": 0, "SlicePosition": -50, "COM9-DelayBetweenCharsMs": "0.0000", "555-Name": "DA Shutter", "PIEZO-LudlSingleAxisName": "B", "XLIGHT Emission Wheel-Label": "725/40 (C)", "640 Shutter-Name": "AnalogIO", "640 AO-Sequenceable": "1", "520 Shutter-Demo": "No", "528 Shutter-Demo": "No", "470-Name": "DA Shutter", "520-DA Device": "520 Shutter", "XYStage-Description": "Ludl XY stage driver adapter", "445 Shutter-MinVolts": "0.0000", "528 AO-TriggerSampleRate": "100000", "405 AO-MinVolts": "0.0000", "405 Shutter-TriggerSequenceLength": "1000", "Height": 1200, "555 AO-Block voltage": "false", "640 Shutter-Volts": "5.0000", "PixelType": "GRAY16", "445 Shutter-Block voltage": "false", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "520 Shutter-TriggerSequenceLength": "1000", "COM9-Name": "COM9", "Core-SLM": "", "555 AO-Volts": "0.0000", "555 Shutter-Block voltage": "false", "XYStage-Speed": "1800.0000", "520 AO-MaxVolts": "5.0000", "528 Shutter-SupportsTriggering": "0", "COM1-Description": "Serial port driver (boost:asio)", "PRIME-MetadataEnabled": "No", "520 Shutter-MinVolts": "0.0000", "470 Shutter-Sequenceable": "1", "PRIME-PreampDelay": "0", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-MinVolts": "0.0000", "ChannelIndex": 3, "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Core-AutoShutter": "1", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "Exposure-ms": 520, "PRIME-PP 2 ENABLED": "Yes", "640 Shutter-TriggerSampleRate": "100000", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "470 Shutter-IOChannel": "Dev1/ao10", "405 AO-Demo": "No", "PixelSizeUm": 1.1, "470 AO-Demo": "No", "470 Shutter-MaxVolts": "5.0000", "Vincent-D3-Name": "Vincent-D3", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FileName": "img_000000000_Il-C_000.tif", "LudlController-Reset": "Operate", "PRIME-Exposure": "520.00", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "445-Name": "DA Shutter", "555 Shutter-Name": "AnalogIO", "520 Shutter-TriggerSampleRate": "100000", "445 Shutter-IOChannel": "Dev1/ao9", "PRIME-TransposeCorrection": "0", "XYStage-StepSize": "0.1000", "445-Description": "DA device that is used as a shutter", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 AO-IOChannel": "Dev1/ao7", "520 Shutter-Name": "AnalogIO", "COM3-DataBits": "8", "640 AO-Volts": "4.5000", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "555 Shutter-IOChannel": "Dev1/ao13", "WaitInterval": null, "520 Shutter-Sequenceable": "1", "YPositionUm": -180, "445-State": "0", "445-DA Device": "445 Shutter", "PRIME-CircularBufferAutoSize": "ON", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "555 AO-TriggerSampleRate": "100000", "445 Shutter-Volts": "0.0000", "XLIGHT Hub-Port": "COM3", "Core-AutoFocus": "", "COM4-Handshaking": "Off", "528 Shutter-MinVolts": "0.0000", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "445 Shutter-Name": "AnalogIO", "528 Shutter-Sequenceable": "1", "XYStage-TransposeMirrorX": "0", "XYStage-TransposeMirrorY": "0", "PRIME-ReadoutRate": "100MHz 16bit", "XYStage-Name": "Stage", "640 AO-SupportsTriggering": "0", "640-Name": "DA Shutter", "640 Shutter-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "640 AO-Block voltage": "false", "445 Shutter-Sequenceable": "1", "470 AO-MaxVolts": "5.0000", "COM4-BaudRate": "9600", "470-Description": "DA device that is used as a shutter", "520 Shutter-Description": "NI DAC", "PRIME-CCDTemperature": "-14.8500", "Time": "2019-12-18 23:11:09 -0500", "640 AO-TriggerSequenceLength": "1000", "470 AO-Name": "AnalogIO", "XLIGHT Disk slider-Label": "Disk Pos 40um", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "Binning": "1x1", "TE2000-ModelType": "1", "COM3-AnswerTimeout": "500.0000", "555 Shutter-SupportsTriggering": "0", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "TE2000-Port": "COM4", "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Description": "NI DAC", "COM4-AnswerTimeout": "500.0000", "COM1-DelayBetweenCharsMs": "0.0000", "FilterBlock-Label": "Confocal", "445 AO-Name": "AnalogIO", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-Timing-ReadoutTimeNs": "24672000.0000", "528 AO-Demo": "No", "520 AO-Sequenceable": "1", "555-Description": "DA device that is used as a shutter", "405-DA Device": "405 Shutter", "405 AO-TriggerSequenceLength": "1000", "640 AO-MinVolts": "0.0000", "640 Shutter-Block voltage": "false", "FrameIndex": 0, "PRIME-Port": "CMOS", "555 AO-MinVolts": "0.0000", "COM3-Description": "Serial port driver (boost:asio)", "528 AO-Description": "NI DAC", "555 AO-IOChannel": "Dev1/ao6", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "555 AO-TriggerInputLine": "/Dev1/PFI0", "PRIME-ActualInterval-ms": "390.5200", "LudlController-Port": "COM9", "445 Shutter-Demo": "No", "PRIME-Trigger-Expose Out-Mux": "1", "SliceIndex": 0, "PRIME-CCDTemperatureSetPoint": "-15.0000", "405 Shutter-MinVolts": "0.0000", "PRIME-ExposeOutMode": "First Row", "AxisPositions": null, "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Volts": "0.0000", "528-Name": "DA Shutter", "555-State": "0", "XLIGHT Dichroic-Label": "Di03-R635-t1", "445 AO-Sequenceable": "1", "405-Name": "DA Shutter", "405 AO-IOChannel": "Dev1/ao0", "Core-Camera": "PRIME", "Focus-Name": "Focus", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "405 Shutter-IOChannel": "Dev1/ao8", "640 AO-Demo": "No", "640 Shutter-SupportsTriggering": "0", "445 AO-Demo": "No", "555-DA Device": "555 Shutter", "470 Shutter-Description": "NI DAC", "528 AO-Volts": "0.0000", "COM1-DataBits": "8", "Vincent-D3-Address": "x", "520 AO-SupportsTriggering": "0", "405-Description": "DA device that is used as a shutter", "640 Shutter-MinVolts": "0.0000", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "405 AO-Name": "AnalogIO", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "640 AO-TriggerInputLine": "/Dev1/PFI0", "405 AO-TriggerInputLine": "/Dev1/PFI0", "640-Description": "DA device that is used as a shutter", "445 Shutter-TriggerSampleRate": "100000", "470 Shutter-TriggerSequenceLength": "1000", "COM1-StopBits": "1", "520 Shutter-SupportsTriggering": "0", "405-State": "0", "Core-Initialize": "1", "XLIGHT Emission Wheel-State": "3", "520 AO-Description": "NI DAC", "528 AO-TriggerInputLine": "/Dev1/PFI0", "445 Shutter-MaxVolts": "5.0000", "COM9-Handshaking": "Off", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "XLIGHT Spin motor-Label": "On (spinning)", "PRIME-PP 5 ENABLED": "No", "UUID": "47abe251-090a-4a50-9f13-7acff1bb049d", "PRIME-TriggerMode": "Internal Trigger", "COM1-Handshaking": "Off", "PRIME-Timing-PostTriggerDelayNs": "49344000.0000", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "Focus-Position": "17741.5000", "528 Shutter-Block voltage": "false", "Channel": "Il-C", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "555 Shutter-Demo": "No", "405 Shutter-Demo": "No", "528 AO-Name": "AnalogIO", "520-State": "0", "Core-Galvo": "", "405 Shutter-TriggerSampleRate": "100000", "Focus-FocusStepSize_(nm)": "100", "PRIME-PixelType": "16bit", "COM4-DataBits": "8", "470 AO-IOChannel": "Dev1/ao2", "520 AO-MinVolts": "0.0000", "COM9-Parity": "None", "PRIME-CircularBufferEnabled": "ON", "PRIME-PP 6 Reset": "No", "640 AO-Description": "NI DAC", "Source": "PRIME", "445 AO-MinVolts": "0.0000", "PIEZO-Name": "Stage", "445 AO-Description": "NI DAC", "PRIME-CircularBufferFrameCount": "46", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "520 AO-Volts": "0.0000", "528 Shutter-TriggerSampleRate": "100000", "XPositionUm": 15712.1, "OpticalPath-State": "4", "PRIME-PP 3 THRESHOLD": "80", "PRIME-PVCAM Version": "3.7.1", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Gain": "1-HDR", "640 Shutter-TriggerSequenceLength": "1000", "528 AO-SupportsTriggering": "0", "445 AO-TriggerInputLine": "/Dev1/PFI0", "OpticalPath-Name": "OpticalPath", "PRIME-PP 4 ENABLED": "Yes", "PRIME-PVCAM Adapter Version": "1.3.29", "COM3-Name": "COM3", "405 AO-MaxVolts": "5.0000", "520 Shutter-Block voltage": "false", "PRIME-Timing-ClearingTimeNs": "49344000.0000", "Core-Focus": "PIEZO", "528 AO-Block voltage": "false", "TE2000-Description": "Nikon TE2000 hub", "528 AO-MinVolts": "0.0000", "COM4-Parity": "None", "Summary": { "Slices": 11, "ChContrastMin": [ 0, 0, 0, 0, 0, 0 ], "PixelAspect": 1, "SlicesFirst": true, "Interval_ms": 0, "Time": "2019-12-18 23:10:50 -0500", "Prefix": "seq_5", "InitialPositionList": [ { "Label": "Pos0", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.21], "Focus": [17741.5], "XYStage": [ 44542.200000000004, 31636.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos1", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.22], "Focus": [17741.4], "XYStage": [ 43801.100000000006, 31637.9 ] }, "GridColumnIndex": 0 }, { "Label": "Pos2", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.5], "XYStage": [ 38561.3, 32040.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos3", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.185], "Focus": [17741.3], "XYStage": [ 37698.4, 32041.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos4", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.16], "Focus": [17741.5], "XYStage": [ 32375.4, 31758.9 ] }, "GridColumnIndex": 0 }, { "Label": "Pos5", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.17000000000002], "Focus": [17741.3], "XYStage": [ 31770.7, 31758.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos6", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.4], "XYStage": [ 26536.800000000003, 31068.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos7", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.205], "Focus": [17741.4], "XYStage": [ 25420.2, 31068.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos8", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.85], "Focus": [17741.3], "XYStage": [ 21059, 31508 ] }, "GridColumnIndex": 0 }, { "Label": "Pos9", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.82], "Focus": [17741.3], "XYStage": [ 19731.9, 31506.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos10", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.79], "Focus": [17741.4], "XYStage": [ 14528.5, 32007.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos11", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.83], "Focus": [17741.2], "XYStage": [ 13830.300000000001, 32009.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos12", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.79], "Focus": [17741.3], "XYStage": [ 8775.300000000001, 32017.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos13", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.815], "Focus": [17741.3], "XYStage": [ 8109.200000000001, 32008.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos14", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.805], "Focus": [17741.3], "XYStage": [ 3483.3, 31908.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos15", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.82], "Focus": [17741.2], "XYStage": [ 2686.8, 31903.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos16", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [211.48000000000002], "Focus": [17741.1], "XYStage": [ -1978.9, 31973.600000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos17", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [211.51500000000001], "Focus": [17741.1], "XYStage": [ -2957.8, 31982.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos18", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.195], "Focus": [17741.5], "XYStage": [ 47747.4, -576.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos19", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.23000000000002], "Focus": [17741.6], "XYStage": [ 47053.9, -576.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos20", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.205], "Focus": [17741.4], "XYStage": [ 41871.200000000004, -479.8 ] }, "GridColumnIndex": 0 }, { "Label": "Pos21", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.4], "XYStage": [ 40882.600000000006, -392 ] }, "GridColumnIndex": 0 }, { "Label": "Pos22", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.24], "Focus": [17741.4], "XYStage": [ 35905.6, -135.3 ] }, "GridColumnIndex": 0 }, { "Label": "Pos23", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.195], "Focus": [17741.5], "XYStage": [ 34990.9, -132.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos24", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.46], "Focus": [17741.4], "XYStage": [ 29678.100000000002, -958.1 ] }, "GridColumnIndex": 0 }, { "Label": "Pos25", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.44], "Focus": [17741.5], "XYStage": [ 28983.600000000002, -939.4000000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos26", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.475], "Focus": [17741.5], "XYStage": [ 23019.7, -122 ] }, "GridColumnIndex": 0 }, { "Label": "Pos27", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.44], "Focus": [17741.7], "XYStage": [ 22428, -116.80000000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos28", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.46], "Focus": [17741.7], "XYStage": [ 16364.900000000001, -181.10000000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos29", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.48000000000002], "Focus": [17741.5], "XYStage": [ 15712.1, -180 ] }, "GridColumnIndex": 0 }, { "Label": "Pos30", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.45000000000002], "Focus": [17741.7], "XYStage": [ 10346.1, 77.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos31", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.49], "Focus": [17741.4], "XYStage": [ 9755.5, 85.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos32", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.475], "Focus": [17741.5], "XYStage": [ 4775.5, 293.90000000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos33", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.445], "Focus": [17741.4], "XYStage": [ 3954.9, 293.90000000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos34", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.455], "Focus": [17741.4], "XYStage": [ -1324.9, 545.6 ] }, "GridColumnIndex": 0 }, { "Label": "Pos35", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.445], "Focus": [17741.3], "XYStage": [ -2097, 576.6 ] }, "GridColumnIndex": 0 } ], "z-step_um": 10, "GridRow": 0, "BitDepth": 16, "Depth": 2, "Channels": 6, "Source": "Micro-Manager", "ChColors": [ -16711681, -256, -65281, -1, -1, -16776961 ], "MetadataVersion": 10, "KeepShutterOpenChannels": true, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536, 65536 ], "CustomIntervals_ms": [], "Height": 1200, "MicroManagerVersion": "1.4.23 20180219", "GridColumn": 0, "UUID": "67598d21-5db5-4842-ab60-f506f7b42c7c", "PixelType": "GRAY16", "Width": 1200, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\Yan\\20191218-YC113-S7S8-miseq", "Frames": 1, "Comment": "", "UserName": "Nikon", "PixelSize_um": 1.1, "ROI": [ 0, 0, 1200, 1200 ], "Date": "2019-12-18", "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC", "P-DAPI-high" ], "KeepShutterOpenSlices": true, "IJType": 1, "ComputerName": "ZadorConfocal2", "Positions": 36, "PositionIndex": 29, "CameraTimeout": 20000, "TimeFirst": false }, "640 AO-Name": "AnalogIO", "Vincent-D3-Command": "Close" }, "FrameKey-0-3-1": { "COM4-StopBits": "1", "528 AO-MaxVolts": "5.0000", "XLIGHT Excitation Wheel-State": "1", "470 Shutter-Name": "AnalogIO", "Core-Shutter": "640", "555 AO-Demo": "No", "XLIGHT Dichroic-State": "2", "XYStage-StepSize-Y": "0.1000", "ZPositionUm": 178.48000000000002, "XYStage-StepSize-X": "0.1000", "PRIME-FirmwareVersion": "20.28", "PRIME-ClearCycles": "2", "520-Name": "DA Shutter", "405 Shutter-Name": "AnalogIO", "PIEZO-StepSize": "0.0050", "PRIME-ClearMode": "Pre-Exposure", "470 AO-MinVolts": "0.0000", "405 AO-SupportsTriggering": "1", "CameraChannelIndex": 0, "XYStage-StartSpeed": "500.0000", "COM9-StopBits": "2", "405 AO-Description": "NI DAC", "COM4-DelayBetweenCharsMs": "0.0000", "445 AO-Block voltage": "false", "520 AO-TriggerInputLine": "/Dev1/PFI0", "555 AO-Description": "NI DAC", "520 AO-TriggerSampleRate": "100000", "COM4-Verbose": "1", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM4-Name": "COM4", "405 Shutter-Volts": "0.0000", "Vincent-D3-Port": "COM1", "PRIME-PP 4 MIN ADU AFFECTED": "200", "470 Shutter-MinVolts": "0.0000", "PRIME-PP 3 ENABLED": "Yes", "470 AO-Description": "NI DAC", "PRIME-Description": "PVCAM API device adapter", "520 Shutter-MaxVolts": "5.0000", "520 Shutter-Volts": "0.0000", "528 AO-Sequenceable": "1", "PRIME-Binning": "1x1", "555 Shutter-MaxVolts": "5.0000", "528 Shutter-TriggerSequenceLength": "1000", "405 Shutter-Block voltage": "false", "XLIGHT Spin motor-State": "1", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "Vincent-D3-Time to open (ms)": "35.0000", "Vincent-D3-Time to close (ms)": "35.0000", "445 AO-TriggerSampleRate": "100000", "640 Shutter-IOChannel": "Dev1/ao14", "555 AO-Sequenceable": "1", "520 AO-Name": "AnalogIO", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "470 AO-Block voltage": "false", "COM1-Verbose": "1", "COM9-AnswerTimeout": "10000.0000", "528 Shutter-Name": "AnalogIO", "PRIME-Trigger Timeout (secs)": "10", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "NextFrame": 0, "470 AO-TriggerSequenceLength": "1000", "PRIME-TransposeXY": "0", "445 AO-TriggerSequenceLength": "1000", "COM1-Name": "COM1", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "COM9-DataBits": "8", "COM1-AnswerTimeout": "500.0000", "Camera": "", "TE2000-Version": "V2.50-V2.50-V2.10", "528 Shutter-Volts": "0.0000", "XLIGHT Hub-Name": "XLIGHT Hub", "PIEZO-Description": "Ludl stage driver adapter", "PositionName": "Pos29", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSampleRate": "100000", "PRIME-FanSpeedSetpoint": "High", "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "405 AO-TriggerSampleRate": "100000", "470 Shutter-Block voltage": "false", "470-State": "0", "COM3-Parity": "None", "405 AO-Volts": "0.0000", "PRIME-TransposeMirrorY": "0", "PRIME-ChipName": "GS144BSI", "470 AO-Volts": "0.0000", "PRIME-TransposeMirrorX": "0", "445 Shutter-SupportsTriggering": "0", "470 Shutter-SupportsTriggering": "0", "XLIGHT Hub-Description": "XLIGHT hub", "PRIME-PreampOffLimit": "10000", "470 AO-Sequenceable": "1", "528 Shutter-MaxVolts": "5.0000", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "Core-XYStage": "XYStage", "470 AO-TriggerSampleRate": "100000", "PRIME-Timing-PreTriggerDelayNs": "24672000.0000", "528-DA Device": "528 Shutter", "Focus-Description": "Nikon TE2000 focus adapter", "FilterBlock-State": "5", "520 AO-Demo": "No", "COM3-DelayBetweenCharsMs": "0.0000", "470 Shutter-Volts": "0.0000", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "PRIME-PP 2 THRESHOLD": "125", "PRIME-PP 4 THRESHOLD": "75", "XYStage-Acceleration": "4.0000", "470 Shutter-Demo": "No", "PRIME-Name": "PMPCIECam00", "640-DA Device": "640 Shutter", "405 AO-Block voltage": "false", "ElapsedTime-ms": 1210373, "PRIME-Offset": "100", "445 AO-IOChannel": "Dev1/ao1", "640 AO-MaxVolts": "5.0000", "PositionIndex": 29, "PRIME-PP 1 ENABLED": "Yes", "COM1-BaudRate": "9600", "640-State": "1", "COM3-Verbose": "1", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "LudlController-TransmissionDelay": "0", "PRIME-FullWellCapacity": "62000", "PRIME-SMARTStreamingEnabled": "No", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "640 Shutter-Description": "NI DAC", "528-Description": "DA device that is used as a shutter", "COM4-Description": "Serial port driver (boost:asio)", "405 Shutter-Description": "NI DAC", "TE2000-Name": "TE2000", "528 Shutter-Description": "NI DAC", "COM9-Description": "Serial port driver (boost:asio)", "LudlController-Name": "LudlController", "445 Shutter-TriggerSequenceLength": "1000", "PRIME-SerialNumber": "A18B203008", "445 AO-Volts": "0.0000", "PRIME-X-dimension": "1200", "PRIME-ShutterCloseDelay": "0", "XLIGHT Disk slider-State": "2", "Position": "Default", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 AO-IOChannel": "Dev1/ao5", "520 AO-IOChannel": "Dev1/ao4", "528 AO-TriggerSequenceLength": "1000", "405 Shutter-MaxVolts": "5.0000", "470-DA Device": "470 Shutter", "PRIME-FTCapable": "No", "Core-ChannelGroup": "Channels", "555 AO-SupportsTriggering": "0", "PRIME-Y-dimension": "1200", "Frame": 0, "555 Shutter-Sequenceable": "1", "PRIME-Timing-ExposureTimeNs": "499998000.0000", "Core-ImageProcessor": "", "528-State": "0", "520 AO-Block voltage": "false", "XLIGHT Excitation Wheel-Label": "QUAD", "640 Shutter-Demo": "No", "COM3-StopBits": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "520 Shutter-IOChannel": "Dev1/ao11", "Width": 1200, "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-SupportsTriggering": "0", "640 AO-TriggerSampleRate": "100000", "520-Description": "DA device that is used as a shutter", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "405 Shutter-Sequenceable": "1", "405 Shutter-SupportsTriggering": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-TriggerSampleRate": "100000", "640 Shutter-MaxVolts": "5.0000", "555 AO-MaxVolts": "5.0000", "520 AO-TriggerSequenceLength": "1000", "COM3-BaudRate": "9600", "COM9-BaudRate": "9600", "Vincent-D3-Channel #": "Ch. #1", "PRIME-PMode": "Normal", "OpticalPath-Label": "Left(100)", "BitDepth": 16, "555 AO-TriggerSequenceLength": "1000", "445 Shutter-Description": "NI DAC", "405 AO-Sequenceable": "1", "PRIME-AcquisitionMethod": "Callbacks", "555 AO-Name": "AnalogIO", "PRIME-ShutterMode": "Pre-Exposure", "COM3-Handshaking": "Off", "445 AO-SupportsTriggering": "0", "ROI": "0-0-1200-1200", "COM9-Verbose": "1", "PRIME-Color": "OFF", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "PRIME-ShutterOpenDelay": "0", "COM1-Parity": "None", "Slice": 1, "SlicePosition": -40, "COM9-DelayBetweenCharsMs": "0.0000", "555-Name": "DA Shutter", "PIEZO-LudlSingleAxisName": "B", "XLIGHT Emission Wheel-Label": "725/40 (C)", "640 Shutter-Name": "AnalogIO", "640 AO-Sequenceable": "1", "520 Shutter-Demo": "No", "528 Shutter-Demo": "No", "470-Name": "DA Shutter", "520-DA Device": "520 Shutter", "XYStage-Description": "Ludl XY stage driver adapter", "445 Shutter-MinVolts": "0.0000", "528 AO-TriggerSampleRate": "100000", "405 AO-MinVolts": "0.0000", "405 Shutter-TriggerSequenceLength": "1000", "Height": 1200, "555 AO-Block voltage": "false", "640 Shutter-Volts": "5.0000", "PixelType": "GRAY16", "445 Shutter-Block voltage": "false", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "520 Shutter-TriggerSequenceLength": "1000", "COM9-Name": "COM9", "Core-SLM": "", "555 AO-Volts": "0.0000", "555 Shutter-Block voltage": "false", "XYStage-Speed": "1800.0000", "520 AO-MaxVolts": "5.0000", "528 Shutter-SupportsTriggering": "0", "COM1-Description": "Serial port driver (boost:asio)", "PRIME-MetadataEnabled": "No", "520 Shutter-MinVolts": "0.0000", "470 Shutter-Sequenceable": "1", "PRIME-PreampDelay": "0", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-MinVolts": "0.0000", "ChannelIndex": 3, "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Core-AutoShutter": "1", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "Exposure-ms": 520, "PRIME-PP 2 ENABLED": "Yes", "640 Shutter-TriggerSampleRate": "100000", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "470 Shutter-IOChannel": "Dev1/ao10", "405 AO-Demo": "No", "PixelSizeUm": 1.1, "470 AO-Demo": "No", "470 Shutter-MaxVolts": "5.0000", "Vincent-D3-Name": "Vincent-D3", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FileName": "img_000000000_Il-C_001.tif", "LudlController-Reset": "Operate", "PRIME-Exposure": "520.00", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "445-Name": "DA Shutter", "555 Shutter-Name": "AnalogIO", "520 Shutter-TriggerSampleRate": "100000", "445 Shutter-IOChannel": "Dev1/ao9", "PRIME-TransposeCorrection": "0", "XYStage-StepSize": "0.1000", "445-Description": "DA device that is used as a shutter", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 AO-IOChannel": "Dev1/ao7", "520 Shutter-Name": "AnalogIO", "COM3-DataBits": "8", "640 AO-Volts": "4.5000", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "555 Shutter-IOChannel": "Dev1/ao13", "WaitInterval": null, "520 Shutter-Sequenceable": "1", "YPositionUm": -180, "445-State": "0", "445-DA Device": "445 Shutter", "PRIME-CircularBufferAutoSize": "ON", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "555 AO-TriggerSampleRate": "100000", "445 Shutter-Volts": "0.0000", "XLIGHT Hub-Port": "COM3", "Core-AutoFocus": "", "COM4-Handshaking": "Off", "528 Shutter-MinVolts": "0.0000", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "445 Shutter-Name": "AnalogIO", "528 Shutter-Sequenceable": "1", "XYStage-TransposeMirrorX": "0", "XYStage-TransposeMirrorY": "0", "PRIME-ReadoutRate": "100MHz 16bit", "XYStage-Name": "Stage", "640 AO-SupportsTriggering": "0", "640-Name": "DA Shutter", "640 Shutter-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "640 AO-Block voltage": "false", "445 Shutter-Sequenceable": "1", "470 AO-MaxVolts": "5.0000", "COM4-BaudRate": "9600", "470-Description": "DA device that is used as a shutter", "520 Shutter-Description": "NI DAC", "PRIME-CCDTemperature": "-14.8500", "Time": "2019-12-18 23:11:10 -0500", "640 AO-TriggerSequenceLength": "1000", "470 AO-Name": "AnalogIO", "XLIGHT Disk slider-Label": "Disk Pos 40um", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "Binning": "1x1", "TE2000-ModelType": "1", "COM3-AnswerTimeout": "500.0000", "555 Shutter-SupportsTriggering": "0", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "TE2000-Port": "COM4", "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Description": "NI DAC", "COM4-AnswerTimeout": "500.0000", "COM1-DelayBetweenCharsMs": "0.0000", "FilterBlock-Label": "Confocal", "445 AO-Name": "AnalogIO", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-Timing-ReadoutTimeNs": "24672000.0000", "528 AO-Demo": "No", "520 AO-Sequenceable": "1", "555-Description": "DA device that is used as a shutter", "405-DA Device": "405 Shutter", "405 AO-TriggerSequenceLength": "1000", "640 AO-MinVolts": "0.0000", "640 Shutter-Block voltage": "false", "FrameIndex": 0, "PRIME-Port": "CMOS", "555 AO-MinVolts": "0.0000", "COM3-Description": "Serial port driver (boost:asio)", "528 AO-Description": "NI DAC", "555 AO-IOChannel": "Dev1/ao6", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "555 AO-TriggerInputLine": "/Dev1/PFI0", "PRIME-ActualInterval-ms": "390.5200", "LudlController-Port": "COM9", "445 Shutter-Demo": "No", "PRIME-Trigger-Expose Out-Mux": "1", "SliceIndex": 1, "PRIME-CCDTemperatureSetPoint": "-15.0000", "405 Shutter-MinVolts": "0.0000", "PRIME-ExposeOutMode": "First Row", "AxisPositions": null, "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Volts": "0.0000", "528-Name": "DA Shutter", "555-State": "0", "XLIGHT Dichroic-Label": "Di03-R635-t1", "445 AO-Sequenceable": "1", "405-Name": "DA Shutter", "405 AO-IOChannel": "Dev1/ao0", "Core-Camera": "PRIME", "Focus-Name": "Focus", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "405 Shutter-IOChannel": "Dev1/ao8", "640 AO-Demo": "No", "640 Shutter-SupportsTriggering": "0", "445 AO-Demo": "No", "555-DA Device": "555 Shutter", "470 Shutter-Description": "NI DAC", "528 AO-Volts": "0.0000", "COM1-DataBits": "8", "Vincent-D3-Address": "x", "520 AO-SupportsTriggering": "0", "405-Description": "DA device that is used as a shutter", "640 Shutter-MinVolts": "0.0000", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "405 AO-Name": "AnalogIO", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "640 AO-TriggerInputLine": "/Dev1/PFI0", "405 AO-TriggerInputLine": "/Dev1/PFI0", "640-Description": "DA device that is used as a shutter", "445 Shutter-TriggerSampleRate": "100000", "470 Shutter-TriggerSequenceLength": "1000", "COM1-StopBits": "1", "520 Shutter-SupportsTriggering": "0", "405-State": "0", "Core-Initialize": "1", "XLIGHT Emission Wheel-State": "3", "520 AO-Description": "NI DAC", "528 AO-TriggerInputLine": "/Dev1/PFI0", "445 Shutter-MaxVolts": "5.0000", "COM9-Handshaking": "Off", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "XLIGHT Spin motor-Label": "On (spinning)", "PRIME-PP 5 ENABLED": "No", "UUID": "1fb485a5-007b-460d-a1a7-520526211d53", "PRIME-TriggerMode": "Internal Trigger", "COM1-Handshaking": "Off", "PRIME-Timing-PostTriggerDelayNs": "49344000.0000", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "Focus-Position": "17741.5000", "528 Shutter-Block voltage": "false", "Channel": "Il-C", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "555 Shutter-Demo": "No", "405 Shutter-Demo": "No", "528 AO-Name": "AnalogIO", "520-State": "0", "Core-Galvo": "", "405 Shutter-TriggerSampleRate": "100000", "Focus-FocusStepSize_(nm)": "100", "PRIME-PixelType": "16bit", "COM4-DataBits": "8", "470 AO-IOChannel": "Dev1/ao2", "520 AO-MinVolts": "0.0000", "COM9-Parity": "None", "PRIME-CircularBufferEnabled": "ON", "PRIME-PP 6 Reset": "No", "640 AO-Description": "NI DAC", "Source": "PRIME", "445 AO-MinVolts": "0.0000", "PIEZO-Name": "Stage", "445 AO-Description": "NI DAC", "PRIME-CircularBufferFrameCount": "46", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "520 AO-Volts": "0.0000", "528 Shutter-TriggerSampleRate": "100000", "XPositionUm": 15712.1, "OpticalPath-State": "4", "PRIME-PP 3 THRESHOLD": "80", "PRIME-PVCAM Version": "3.7.1", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Gain": "1-HDR", "640 Shutter-TriggerSequenceLength": "1000", "528 AO-SupportsTriggering": "0", "445 AO-TriggerInputLine": "/Dev1/PFI0", "OpticalPath-Name": "OpticalPath", "PRIME-PP 4 ENABLED": "Yes", "PRIME-PVCAM Adapter Version": "1.3.29", "COM3-Name": "COM3", "405 AO-MaxVolts": "5.0000", "520 Shutter-Block voltage": "false", "PRIME-Timing-ClearingTimeNs": "49344000.0000", "Core-Focus": "PIEZO", "528 AO-Block voltage": "false", "TE2000-Description": "Nikon TE2000 hub", "528 AO-MinVolts": "0.0000", "COM4-Parity": "None", "Summary": { "Slices": 11, "ChContrastMin": [ 0, 0, 0, 0, 0, 0 ], "PixelAspect": 1, "SlicesFirst": true, "Interval_ms": 0, "Time": "2019-12-18 23:10:50 -0500", "Prefix": "seq_5", "InitialPositionList": [ { "Label": "Pos0", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.21], "Focus": [17741.5], "XYStage": [ 44542.200000000004, 31636.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos1", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.22], "Focus": [17741.4], "XYStage": [ 43801.100000000006, 31637.9 ] }, "GridColumnIndex": 0 }, { "Label": "Pos2", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.5], "XYStage": [ 38561.3, 32040.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos3", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.185], "Focus": [17741.3], "XYStage": [ 37698.4, 32041.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos4", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.16], "Focus": [17741.5], "XYStage": [ 32375.4, 31758.9 ] }, "GridColumnIndex": 0 }, { "Label": "Pos5", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.17000000000002], "Focus": [17741.3], "XYStage": [ 31770.7, 31758.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos6", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.4], "XYStage": [ 26536.800000000003, 31068.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos7", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.205], "Focus": [17741.4], "XYStage": [ 25420.2, 31068.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos8", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.85], "Focus": [17741.3], "XYStage": [ 21059, 31508 ] }, "GridColumnIndex": 0 }, { "Label": "Pos9", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.82], "Focus": [17741.3], "XYStage": [ 19731.9, 31506.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos10", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.79], "Focus": [17741.4], "XYStage": [ 14528.5, 32007.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos11", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.83], "Focus": [17741.2], "XYStage": [ 13830.300000000001, 32009.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos12", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.79], "Focus": [17741.3], "XYStage": [ 8775.300000000001, 32017.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos13", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.815], "Focus": [17741.3], "XYStage": [ 8109.200000000001, 32008.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos14", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.805], "Focus": [17741.3], "XYStage": [ 3483.3, 31908.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos15", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.82], "Focus": [17741.2], "XYStage": [ 2686.8, 31903.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos16", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [211.48000000000002], "Focus": [17741.1], "XYStage": [ -1978.9, 31973.600000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos17", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [211.51500000000001], "Focus": [17741.1], "XYStage": [ -2957.8, 31982.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos18", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.195], "Focus": [17741.5], "XYStage": [ 47747.4, -576.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos19", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.23000000000002], "Focus": [17741.6], "XYStage": [ 47053.9, -576.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos20", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.205], "Focus": [17741.4], "XYStage": [ 41871.200000000004, -479.8 ] }, "GridColumnIndex": 0 }, { "Label": "Pos21", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.4], "XYStage": [ 40882.600000000006, -392 ] }, "GridColumnIndex": 0 }, { "Label": "Pos22", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.24], "Focus": [17741.4], "XYStage": [ 35905.6, -135.3 ] }, "GridColumnIndex": 0 }, { "Label": "Pos23", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.195], "Focus": [17741.5], "XYStage": [ 34990.9, -132.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos24", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.46], "Focus": [17741.4], "XYStage": [ 29678.100000000002, -958.1 ] }, "GridColumnIndex": 0 }, { "Label": "Pos25", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.44], "Focus": [17741.5], "XYStage": [ 28983.600000000002, -939.4000000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos26", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.475], "Focus": [17741.5], "XYStage": [ 23019.7, -122 ] }, "GridColumnIndex": 0 }, { "Label": "Pos27", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.44], "Focus": [17741.7], "XYStage": [ 22428, -116.80000000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos28", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.46], "Focus": [17741.7], "XYStage": [ 16364.900000000001, -181.10000000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos29", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.48000000000002], "Focus": [17741.5], "XYStage": [ 15712.1, -180 ] }, "GridColumnIndex": 0 }, { "Label": "Pos30", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.45000000000002], "Focus": [17741.7], "XYStage": [ 10346.1, 77.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos31", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.49], "Focus": [17741.4], "XYStage": [ 9755.5, 85.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos32", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.475], "Focus": [17741.5], "XYStage": [ 4775.5, 293.90000000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos33", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.445], "Focus": [17741.4], "XYStage": [ 3954.9, 293.90000000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos34", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.455], "Focus": [17741.4], "XYStage": [ -1324.9, 545.6 ] }, "GridColumnIndex": 0 }, { "Label": "Pos35", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.445], "Focus": [17741.3], "XYStage": [ -2097, 576.6 ] }, "GridColumnIndex": 0 } ], "z-step_um": 10, "GridRow": 0, "BitDepth": 16, "Depth": 2, "Channels": 6, "Source": "Micro-Manager", "ChColors": [ -16711681, -256, -65281, -1, -1, -16776961 ], "MetadataVersion": 10, "KeepShutterOpenChannels": true, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536, 65536 ], "CustomIntervals_ms": [], "Height": 1200, "MicroManagerVersion": "1.4.23 20180219", "GridColumn": 0, "UUID": "67598d21-5db5-4842-ab60-f506f7b42c7c", "PixelType": "GRAY16", "Width": 1200, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\Yan\\20191218-YC113-S7S8-miseq", "Frames": 1, "Comment": "", "UserName": "Nikon", "PixelSize_um": 1.1, "ROI": [ 0, 0, 1200, 1200 ], "Date": "2019-12-18", "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC", "P-DAPI-high" ], "KeepShutterOpenSlices": true, "IJType": 1, "ComputerName": "ZadorConfocal2", "Positions": 36, "PositionIndex": 29, "CameraTimeout": 20000, "TimeFirst": false }, "640 AO-Name": "AnalogIO", "Vincent-D3-Command": "Close" }, "FrameKey-0-3-2": { "COM4-StopBits": "1", "528 AO-MaxVolts": "5.0000", "XLIGHT Excitation Wheel-State": "1", "470 Shutter-Name": "AnalogIO", "Core-Shutter": "640", "555 AO-Demo": "No", "XLIGHT Dichroic-State": "2", "XYStage-StepSize-Y": "0.1000", "ZPositionUm": 188.48000000000002, "XYStage-StepSize-X": "0.1000", "PRIME-FirmwareVersion": "20.28", "PRIME-ClearCycles": "2", "520-Name": "DA Shutter", "405 Shutter-Name": "AnalogIO", "PIEZO-StepSize": "0.0050", "PRIME-ClearMode": "Pre-Exposure", "470 AO-MinVolts": "0.0000", "405 AO-SupportsTriggering": "1", "CameraChannelIndex": 0, "XYStage-StartSpeed": "500.0000", "COM9-StopBits": "2", "405 AO-Description": "NI DAC", "COM4-DelayBetweenCharsMs": "0.0000", "445 AO-Block voltage": "false", "520 AO-TriggerInputLine": "/Dev1/PFI0", "555 AO-Description": "NI DAC", "520 AO-TriggerSampleRate": "100000", "COM4-Verbose": "1", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM4-Name": "COM4", "405 Shutter-Volts": "0.0000", "Vincent-D3-Port": "COM1", "PRIME-PP 4 MIN ADU AFFECTED": "200", "470 Shutter-MinVolts": "0.0000", "PRIME-PP 3 ENABLED": "Yes", "470 AO-Description": "NI DAC", "PRIME-Description": "PVCAM API device adapter", "520 Shutter-MaxVolts": "5.0000", "520 Shutter-Volts": "0.0000", "528 AO-Sequenceable": "1", "PRIME-Binning": "1x1", "555 Shutter-MaxVolts": "5.0000", "528 Shutter-TriggerSequenceLength": "1000", "405 Shutter-Block voltage": "false", "XLIGHT Spin motor-State": "1", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "Vincent-D3-Time to open (ms)": "35.0000", "Vincent-D3-Time to close (ms)": "35.0000", "445 AO-TriggerSampleRate": "100000", "640 Shutter-IOChannel": "Dev1/ao14", "555 AO-Sequenceable": "1", "520 AO-Name": "AnalogIO", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "470 AO-Block voltage": "false", "COM1-Verbose": "1", "COM9-AnswerTimeout": "10000.0000", "528 Shutter-Name": "AnalogIO", "PRIME-Trigger Timeout (secs)": "10", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "NextFrame": 0, "470 AO-TriggerSequenceLength": "1000", "PRIME-TransposeXY": "0", "445 AO-TriggerSequenceLength": "1000", "COM1-Name": "COM1", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "COM9-DataBits": "8", "COM1-AnswerTimeout": "500.0000", "Camera": "", "TE2000-Version": "V2.50-V2.50-V2.10", "528 Shutter-Volts": "0.0000", "XLIGHT Hub-Name": "XLIGHT Hub", "PIEZO-Description": "Ludl stage driver adapter", "PositionName": "Pos29", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSampleRate": "100000", "PRIME-FanSpeedSetpoint": "High", "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "405 AO-TriggerSampleRate": "100000", "470 Shutter-Block voltage": "false", "470-State": "0", "COM3-Parity": "None", "405 AO-Volts": "0.0000", "PRIME-TransposeMirrorY": "0", "PRIME-ChipName": "GS144BSI", "470 AO-Volts": "0.0000", "PRIME-TransposeMirrorX": "0", "445 Shutter-SupportsTriggering": "0", "470 Shutter-SupportsTriggering": "0", "XLIGHT Hub-Description": "XLIGHT hub", "PRIME-PreampOffLimit": "10000", "470 AO-Sequenceable": "1", "528 Shutter-MaxVolts": "5.0000", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "Core-XYStage": "XYStage", "470 AO-TriggerSampleRate": "100000", "PRIME-Timing-PreTriggerDelayNs": "24672000.0000", "528-DA Device": "528 Shutter", "Focus-Description": "Nikon TE2000 focus adapter", "FilterBlock-State": "5", "520 AO-Demo": "No", "COM3-DelayBetweenCharsMs": "0.0000", "470 Shutter-Volts": "0.0000", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "PRIME-PP 2 THRESHOLD": "125", "PRIME-PP 4 THRESHOLD": "75", "XYStage-Acceleration": "4.0000", "470 Shutter-Demo": "No", "PRIME-Name": "PMPCIECam00", "640-DA Device": "640 Shutter", "405 AO-Block voltage": "false", "ElapsedTime-ms": 1211136, "PRIME-Offset": "100", "445 AO-IOChannel": "Dev1/ao1", "640 AO-MaxVolts": "5.0000", "PositionIndex": 29, "PRIME-PP 1 ENABLED": "Yes", "COM1-BaudRate": "9600", "640-State": "1", "COM3-Verbose": "1", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "LudlController-TransmissionDelay": "0", "PRIME-FullWellCapacity": "62000", "PRIME-SMARTStreamingEnabled": "No", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "640 Shutter-Description": "NI DAC", "528-Description": "DA device that is used as a shutter", "COM4-Description": "Serial port driver (boost:asio)", "405 Shutter-Description": "NI DAC", "TE2000-Name": "TE2000", "528 Shutter-Description": "NI DAC", "COM9-Description": "Serial port driver (boost:asio)", "LudlController-Name": "LudlController", "445 Shutter-TriggerSequenceLength": "1000", "PRIME-SerialNumber": "A18B203008", "445 AO-Volts": "0.0000", "PRIME-X-dimension": "1200", "PRIME-ShutterCloseDelay": "0", "XLIGHT Disk slider-State": "2", "Position": "Default", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 AO-IOChannel": "Dev1/ao5", "520 AO-IOChannel": "Dev1/ao4", "528 AO-TriggerSequenceLength": "1000", "405 Shutter-MaxVolts": "5.0000", "470-DA Device": "470 Shutter", "PRIME-FTCapable": "No", "Core-ChannelGroup": "Channels", "555 AO-SupportsTriggering": "0", "PRIME-Y-dimension": "1200", "Frame": 0, "555 Shutter-Sequenceable": "1", "PRIME-Timing-ExposureTimeNs": "499998000.0000", "Core-ImageProcessor": "", "528-State": "0", "520 AO-Block voltage": "false", "XLIGHT Excitation Wheel-Label": "QUAD", "640 Shutter-Demo": "No", "COM3-StopBits": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "520 Shutter-IOChannel": "Dev1/ao11", "Width": 1200, "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-SupportsTriggering": "0", "640 AO-TriggerSampleRate": "100000", "520-Description": "DA device that is used as a shutter", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "405 Shutter-Sequenceable": "1", "405 Shutter-SupportsTriggering": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-TriggerSampleRate": "100000", "640 Shutter-MaxVolts": "5.0000", "555 AO-MaxVolts": "5.0000", "520 AO-TriggerSequenceLength": "1000", "COM3-BaudRate": "9600", "COM9-BaudRate": "9600", "Vincent-D3-Channel #": "Ch. #1", "PRIME-PMode": "Normal", "OpticalPath-Label": "Left(100)", "BitDepth": 16, "555 AO-TriggerSequenceLength": "1000", "445 Shutter-Description": "NI DAC", "405 AO-Sequenceable": "1", "PRIME-AcquisitionMethod": "Callbacks", "555 AO-Name": "AnalogIO", "PRIME-ShutterMode": "Pre-Exposure", "COM3-Handshaking": "Off", "445 AO-SupportsTriggering": "0", "ROI": "0-0-1200-1200", "COM9-Verbose": "1", "PRIME-Color": "OFF", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "PRIME-ShutterOpenDelay": "0", "COM1-Parity": "None", "Slice": 2, "SlicePosition": -30, "COM9-DelayBetweenCharsMs": "0.0000", "555-Name": "DA Shutter", "PIEZO-LudlSingleAxisName": "B", "XLIGHT Emission Wheel-Label": "725/40 (C)", "640 Shutter-Name": "AnalogIO", "640 AO-Sequenceable": "1", "520 Shutter-Demo": "No", "528 Shutter-Demo": "No", "470-Name": "DA Shutter", "520-DA Device": "520 Shutter", "XYStage-Description": "Ludl XY stage driver adapter", "445 Shutter-MinVolts": "0.0000", "528 AO-TriggerSampleRate": "100000", "405 AO-MinVolts": "0.0000", "405 Shutter-TriggerSequenceLength": "1000", "Height": 1200, "555 AO-Block voltage": "false", "640 Shutter-Volts": "5.0000", "PixelType": "GRAY16", "445 Shutter-Block voltage": "false", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "520 Shutter-TriggerSequenceLength": "1000", "COM9-Name": "COM9", "Core-SLM": "", "555 AO-Volts": "0.0000", "555 Shutter-Block voltage": "false", "XYStage-Speed": "1800.0000", "520 AO-MaxVolts": "5.0000", "528 Shutter-SupportsTriggering": "0", "COM1-Description": "Serial port driver (boost:asio)", "PRIME-MetadataEnabled": "No", "520 Shutter-MinVolts": "0.0000", "470 Shutter-Sequenceable": "1", "PRIME-PreampDelay": "0", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-MinVolts": "0.0000", "ChannelIndex": 3, "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Core-AutoShutter": "1", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "Exposure-ms": 520, "PRIME-PP 2 ENABLED": "Yes", "640 Shutter-TriggerSampleRate": "100000", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "470 Shutter-IOChannel": "Dev1/ao10", "405 AO-Demo": "No", "PixelSizeUm": 1.1, "470 AO-Demo": "No", "470 Shutter-MaxVolts": "5.0000", "Vincent-D3-Name": "Vincent-D3", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FileName": "img_000000000_Il-C_002.tif", "LudlController-Reset": "Operate", "PRIME-Exposure": "520.00", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "445-Name": "DA Shutter", "555 Shutter-Name": "AnalogIO", "520 Shutter-TriggerSampleRate": "100000", "445 Shutter-IOChannel": "Dev1/ao9", "PRIME-TransposeCorrection": "0", "XYStage-StepSize": "0.1000", "445-Description": "DA device that is used as a shutter", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 AO-IOChannel": "Dev1/ao7", "520 Shutter-Name": "AnalogIO", "COM3-DataBits": "8", "640 AO-Volts": "4.5000", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "555 Shutter-IOChannel": "Dev1/ao13", "WaitInterval": null, "520 Shutter-Sequenceable": "1", "YPositionUm": -180, "445-State": "0", "445-DA Device": "445 Shutter", "PRIME-CircularBufferAutoSize": "ON", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "555 AO-TriggerSampleRate": "100000", "445 Shutter-Volts": "0.0000", "XLIGHT Hub-Port": "COM3", "Core-AutoFocus": "", "COM4-Handshaking": "Off", "528 Shutter-MinVolts": "0.0000", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "445 Shutter-Name": "AnalogIO", "528 Shutter-Sequenceable": "1", "XYStage-TransposeMirrorX": "0", "XYStage-TransposeMirrorY": "0", "PRIME-ReadoutRate": "100MHz 16bit", "XYStage-Name": "Stage", "640 AO-SupportsTriggering": "0", "640-Name": "DA Shutter", "640 Shutter-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "640 AO-Block voltage": "false", "445 Shutter-Sequenceable": "1", "470 AO-MaxVolts": "5.0000", "COM4-BaudRate": "9600", "470-Description": "DA device that is used as a shutter", "520 Shutter-Description": "NI DAC", "PRIME-CCDTemperature": "-14.8500", "Time": "2019-12-18 23:11:11 -0500", "640 AO-TriggerSequenceLength": "1000", "470 AO-Name": "AnalogIO", "XLIGHT Disk slider-Label": "Disk Pos 40um", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "Binning": "1x1", "TE2000-ModelType": "1", "COM3-AnswerTimeout": "500.0000", "555 Shutter-SupportsTriggering": "0", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "TE2000-Port": "COM4", "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Description": "NI DAC", "COM4-AnswerTimeout": "500.0000", "COM1-DelayBetweenCharsMs": "0.0000", "FilterBlock-Label": "Confocal", "445 AO-Name": "AnalogIO", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-Timing-ReadoutTimeNs": "24672000.0000", "528 AO-Demo": "No", "520 AO-Sequenceable": "1", "555-Description": "DA device that is used as a shutter", "405-DA Device": "405 Shutter", "405 AO-TriggerSequenceLength": "1000", "640 AO-MinVolts": "0.0000", "640 Shutter-Block voltage": "false", "FrameIndex": 0, "PRIME-Port": "CMOS", "555 AO-MinVolts": "0.0000", "COM3-Description": "Serial port driver (boost:asio)", "528 AO-Description": "NI DAC", "555 AO-IOChannel": "Dev1/ao6", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "555 AO-TriggerInputLine": "/Dev1/PFI0", "PRIME-ActualInterval-ms": "390.5200", "LudlController-Port": "COM9", "445 Shutter-Demo": "No", "PRIME-Trigger-Expose Out-Mux": "1", "SliceIndex": 2, "PRIME-CCDTemperatureSetPoint": "-15.0000", "405 Shutter-MinVolts": "0.0000", "PRIME-ExposeOutMode": "First Row", "AxisPositions": null, "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Volts": "0.0000", "528-Name": "DA Shutter", "555-State": "0", "XLIGHT Dichroic-Label": "Di03-R635-t1", "445 AO-Sequenceable": "1", "405-Name": "DA Shutter", "405 AO-IOChannel": "Dev1/ao0", "Core-Camera": "PRIME", "Focus-Name": "Focus", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "405 Shutter-IOChannel": "Dev1/ao8", "640 AO-Demo": "No", "640 Shutter-SupportsTriggering": "0", "445 AO-Demo": "No", "555-DA Device": "555 Shutter", "470 Shutter-Description": "NI DAC", "528 AO-Volts": "0.0000", "COM1-DataBits": "8", "Vincent-D3-Address": "x", "520 AO-SupportsTriggering": "0", "405-Description": "DA device that is used as a shutter", "640 Shutter-MinVolts": "0.0000", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "405 AO-Name": "AnalogIO", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "640 AO-TriggerInputLine": "/Dev1/PFI0", "405 AO-TriggerInputLine": "/Dev1/PFI0", "640-Description": "DA device that is used as a shutter", "445 Shutter-TriggerSampleRate": "100000", "470 Shutter-TriggerSequenceLength": "1000", "COM1-StopBits": "1", "520 Shutter-SupportsTriggering": "0", "405-State": "0", "Core-Initialize": "1", "XLIGHT Emission Wheel-State": "3", "520 AO-Description": "NI DAC", "528 AO-TriggerInputLine": "/Dev1/PFI0", "445 Shutter-MaxVolts": "5.0000", "COM9-Handshaking": "Off", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "XLIGHT Spin motor-Label": "On (spinning)", "PRIME-PP 5 ENABLED": "No", "UUID": "a80834e9-9450-4e9e-a1a7-2bc66228b1a0", "PRIME-TriggerMode": "Internal Trigger", "COM1-Handshaking": "Off", "PRIME-Timing-PostTriggerDelayNs": "49344000.0000", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "Focus-Position": "17741.5000", "528 Shutter-Block voltage": "false", "Channel": "Il-C", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "555 Shutter-Demo": "No", "405 Shutter-Demo": "No", "528 AO-Name": "AnalogIO", "520-State": "0", "Core-Galvo": "", "405 Shutter-TriggerSampleRate": "100000", "Focus-FocusStepSize_(nm)": "100", "PRIME-PixelType": "16bit", "COM4-DataBits": "8", "470 AO-IOChannel": "Dev1/ao2", "520 AO-MinVolts": "0.0000", "COM9-Parity": "None", "PRIME-CircularBufferEnabled": "ON", "PRIME-PP 6 Reset": "No", "640 AO-Description": "NI DAC", "Source": "PRIME", "445 AO-MinVolts": "0.0000", "PIEZO-Name": "Stage", "445 AO-Description": "NI DAC", "PRIME-CircularBufferFrameCount": "46", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "520 AO-Volts": "0.0000", "528 Shutter-TriggerSampleRate": "100000", "XPositionUm": 15712.1, "OpticalPath-State": "4", "PRIME-PP 3 THRESHOLD": "80", "PRIME-PVCAM Version": "3.7.1", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Gain": "1-HDR", "640 Shutter-TriggerSequenceLength": "1000", "528 AO-SupportsTriggering": "0", "445 AO-TriggerInputLine": "/Dev1/PFI0", "OpticalPath-Name": "OpticalPath", "PRIME-PP 4 ENABLED": "Yes", "PRIME-PVCAM Adapter Version": "1.3.29", "COM3-Name": "COM3", "405 AO-MaxVolts": "5.0000", "520 Shutter-Block voltage": "false", "PRIME-Timing-ClearingTimeNs": "49344000.0000", "Core-Focus": "PIEZO", "528 AO-Block voltage": "false", "TE2000-Description": "Nikon TE2000 hub", "528 AO-MinVolts": "0.0000", "COM4-Parity": "None", "Summary": { "Slices": 11, "ChContrastMin": [ 0, 0, 0, 0, 0, 0 ], "PixelAspect": 1, "SlicesFirst": true, "Interval_ms": 0, "Time": "2019-12-18 23:10:50 -0500", "Prefix": "seq_5", "InitialPositionList": [ { "Label": "Pos0", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.21], "Focus": [17741.5], "XYStage": [ 44542.200000000004, 31636.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos1", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.22], "Focus": [17741.4], "XYStage": [ 43801.100000000006, 31637.9 ] }, "GridColumnIndex": 0 }, { "Label": "Pos2", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.5], "XYStage": [ 38561.3, 32040.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos3", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.185], "Focus": [17741.3], "XYStage": [ 37698.4, 32041.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos4", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.16], "Focus": [17741.5], "XYStage": [ 32375.4, 31758.9 ] }, "GridColumnIndex": 0 }, { "Label": "Pos5", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.17000000000002], "Focus": [17741.3], "XYStage": [ 31770.7, 31758.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos6", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.4], "XYStage": [ 26536.800000000003, 31068.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos7", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.205], "Focus": [17741.4], "XYStage": [ 25420.2, 31068.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos8", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.85], "Focus": [17741.3], "XYStage": [ 21059, 31508 ] }, "GridColumnIndex": 0 }, { "Label": "Pos9", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.82], "Focus": [17741.3], "XYStage": [ 19731.9, 31506.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos10", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.79], "Focus": [17741.4], "XYStage": [ 14528.5, 32007.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos11", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.83], "Focus": [17741.2], "XYStage": [ 13830.300000000001, 32009.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos12", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.79], "Focus": [17741.3], "XYStage": [ 8775.300000000001, 32017.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos13", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.815], "Focus": [17741.3], "XYStage": [ 8109.200000000001, 32008.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos14", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.805], "Focus": [17741.3], "XYStage": [ 3483.3, 31908.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos15", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.82], "Focus": [17741.2], "XYStage": [ 2686.8, 31903.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos16", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [211.48000000000002], "Focus": [17741.1], "XYStage": [ -1978.9, 31973.600000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos17", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [211.51500000000001], "Focus": [17741.1], "XYStage": [ -2957.8, 31982.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos18", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.195], "Focus": [17741.5], "XYStage": [ 47747.4, -576.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos19", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.23000000000002], "Focus": [17741.6], "XYStage": [ 47053.9, -576.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos20", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.205], "Focus": [17741.4], "XYStage": [ 41871.200000000004, -479.8 ] }, "GridColumnIndex": 0 }, { "Label": "Pos21", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.4], "XYStage": [ 40882.600000000006, -392 ] }, "GridColumnIndex": 0 }, { "Label": "Pos22", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.24], "Focus": [17741.4], "XYStage": [ 35905.6, -135.3 ] }, "GridColumnIndex": 0 }, { "Label": "Pos23", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.195], "Focus": [17741.5], "XYStage": [ 34990.9, -132.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos24", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.46], "Focus": [17741.4], "XYStage": [ 29678.100000000002, -958.1 ] }, "GridColumnIndex": 0 }, { "Label": "Pos25", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.44], "Focus": [17741.5], "XYStage": [ 28983.600000000002, -939.4000000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos26", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.475], "Focus": [17741.5], "XYStage": [ 23019.7, -122 ] }, "GridColumnIndex": 0 }, { "Label": "Pos27", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.44], "Focus": [17741.7], "XYStage": [ 22428, -116.80000000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos28", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.46], "Focus": [17741.7], "XYStage": [ 16364.900000000001, -181.10000000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos29", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.48000000000002], "Focus": [17741.5], "XYStage": [ 15712.1, -180 ] }, "GridColumnIndex": 0 }, { "Label": "Pos30", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.45000000000002], "Focus": [17741.7], "XYStage": [ 10346.1, 77.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos31", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.49], "Focus": [17741.4], "XYStage": [ 9755.5, 85.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos32", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.475], "Focus": [17741.5], "XYStage": [ 4775.5, 293.90000000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos33", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.445], "Focus": [17741.4], "XYStage": [ 3954.9, 293.90000000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos34", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.455], "Focus": [17741.4], "XYStage": [ -1324.9, 545.6 ] }, "GridColumnIndex": 0 }, { "Label": "Pos35", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.445], "Focus": [17741.3], "XYStage": [ -2097, 576.6 ] }, "GridColumnIndex": 0 } ], "z-step_um": 10, "GridRow": 0, "BitDepth": 16, "Depth": 2, "Channels": 6, "Source": "Micro-Manager", "ChColors": [ -16711681, -256, -65281, -1, -1, -16776961 ], "MetadataVersion": 10, "KeepShutterOpenChannels": true, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536, 65536 ], "CustomIntervals_ms": [], "Height": 1200, "MicroManagerVersion": "1.4.23 20180219", "GridColumn": 0, "UUID": "67598d21-5db5-4842-ab60-f506f7b42c7c", "PixelType": "GRAY16", "Width": 1200, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\Yan\\20191218-YC113-S7S8-miseq", "Frames": 1, "Comment": "", "UserName": "Nikon", "PixelSize_um": 1.1, "ROI": [ 0, 0, 1200, 1200 ], "Date": "2019-12-18", "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC", "P-DAPI-high" ], "KeepShutterOpenSlices": true, "IJType": 1, "ComputerName": "ZadorConfocal2", "Positions": 36, "PositionIndex": 29, "CameraTimeout": 20000, "TimeFirst": false }, "640 AO-Name": "AnalogIO", "Vincent-D3-Command": "Close" }, "FrameKey-0-3-3": { "COM4-StopBits": "1", "528 AO-MaxVolts": "5.0000", "XLIGHT Excitation Wheel-State": "1", "470 Shutter-Name": "AnalogIO", "Core-Shutter": "640", "555 AO-Demo": "No", "XLIGHT Dichroic-State": "2", "XYStage-StepSize-Y": "0.1000", "ZPositionUm": 198.48000000000002, "XYStage-StepSize-X": "0.1000", "PRIME-FirmwareVersion": "20.28", "PRIME-ClearCycles": "2", "520-Name": "DA Shutter", "405 Shutter-Name": "AnalogIO", "PIEZO-StepSize": "0.0050", "PRIME-ClearMode": "Pre-Exposure", "470 AO-MinVolts": "0.0000", "405 AO-SupportsTriggering": "1", "CameraChannelIndex": 0, "XYStage-StartSpeed": "500.0000", "COM9-StopBits": "2", "405 AO-Description": "NI DAC", "COM4-DelayBetweenCharsMs": "0.0000", "445 AO-Block voltage": "false", "520 AO-TriggerInputLine": "/Dev1/PFI0", "555 AO-Description": "NI DAC", "520 AO-TriggerSampleRate": "100000", "COM4-Verbose": "1", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM4-Name": "COM4", "405 Shutter-Volts": "0.0000", "Vincent-D3-Port": "COM1", "PRIME-PP 4 MIN ADU AFFECTED": "200", "470 Shutter-MinVolts": "0.0000", "PRIME-PP 3 ENABLED": "Yes", "470 AO-Description": "NI DAC", "PRIME-Description": "PVCAM API device adapter", "520 Shutter-MaxVolts": "5.0000", "520 Shutter-Volts": "0.0000", "528 AO-Sequenceable": "1", "PRIME-Binning": "1x1", "555 Shutter-MaxVolts": "5.0000", "528 Shutter-TriggerSequenceLength": "1000", "405 Shutter-Block voltage": "false", "XLIGHT Spin motor-State": "1", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "Vincent-D3-Time to open (ms)": "35.0000", "Vincent-D3-Time to close (ms)": "35.0000", "445 AO-TriggerSampleRate": "100000", "640 Shutter-IOChannel": "Dev1/ao14", "555 AO-Sequenceable": "1", "520 AO-Name": "AnalogIO", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "470 AO-Block voltage": "false", "COM1-Verbose": "1", "COM9-AnswerTimeout": "10000.0000", "528 Shutter-Name": "AnalogIO", "PRIME-Trigger Timeout (secs)": "10", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "NextFrame": 0, "470 AO-TriggerSequenceLength": "1000", "PRIME-TransposeXY": "0", "445 AO-TriggerSequenceLength": "1000", "COM1-Name": "COM1", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "COM9-DataBits": "8", "COM1-AnswerTimeout": "500.0000", "Camera": "", "TE2000-Version": "V2.50-V2.50-V2.10", "528 Shutter-Volts": "0.0000", "XLIGHT Hub-Name": "XLIGHT Hub", "PIEZO-Description": "Ludl stage driver adapter", "PositionName": "Pos29", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSampleRate": "100000", "PRIME-FanSpeedSetpoint": "High", "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "405 AO-TriggerSampleRate": "100000", "470 Shutter-Block voltage": "false", "470-State": "0", "COM3-Parity": "None", "405 AO-Volts": "0.0000", "PRIME-TransposeMirrorY": "0", "PRIME-ChipName": "GS144BSI", "470 AO-Volts": "0.0000", "PRIME-TransposeMirrorX": "0", "445 Shutter-SupportsTriggering": "0", "470 Shutter-SupportsTriggering": "0", "XLIGHT Hub-Description": "XLIGHT hub", "PRIME-PreampOffLimit": "10000", "470 AO-Sequenceable": "1", "528 Shutter-MaxVolts": "5.0000", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "Core-XYStage": "XYStage", "470 AO-TriggerSampleRate": "100000", "PRIME-Timing-PreTriggerDelayNs": "24672000.0000", "528-DA Device": "528 Shutter", "Focus-Description": "Nikon TE2000 focus adapter", "FilterBlock-State": "5", "520 AO-Demo": "No", "COM3-DelayBetweenCharsMs": "0.0000", "470 Shutter-Volts": "0.0000", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "PRIME-PP 2 THRESHOLD": "125", "PRIME-PP 4 THRESHOLD": "75", "XYStage-Acceleration": "4.0000", "470 Shutter-Demo": "No", "PRIME-Name": "PMPCIECam00", "640-DA Device": "640 Shutter", "405 AO-Block voltage": "false", "ElapsedTime-ms": 1211888, "PRIME-Offset": "100", "445 AO-IOChannel": "Dev1/ao1", "640 AO-MaxVolts": "5.0000", "PositionIndex": 29, "PRIME-PP 1 ENABLED": "Yes", "COM1-BaudRate": "9600", "640-State": "1", "COM3-Verbose": "1", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "LudlController-TransmissionDelay": "0", "PRIME-FullWellCapacity": "62000", "PRIME-SMARTStreamingEnabled": "No", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "640 Shutter-Description": "NI DAC", "528-Description": "DA device that is used as a shutter", "COM4-Description": "Serial port driver (boost:asio)", "405 Shutter-Description": "NI DAC", "TE2000-Name": "TE2000", "528 Shutter-Description": "NI DAC", "COM9-Description": "Serial port driver (boost:asio)", "LudlController-Name": "LudlController", "445 Shutter-TriggerSequenceLength": "1000", "PRIME-SerialNumber": "A18B203008", "445 AO-Volts": "0.0000", "PRIME-X-dimension": "1200", "PRIME-ShutterCloseDelay": "0", "XLIGHT Disk slider-State": "2", "Position": "Default", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 AO-IOChannel": "Dev1/ao5", "520 AO-IOChannel": "Dev1/ao4", "528 AO-TriggerSequenceLength": "1000", "405 Shutter-MaxVolts": "5.0000", "470-DA Device": "470 Shutter", "PRIME-FTCapable": "No", "Core-ChannelGroup": "Channels", "555 AO-SupportsTriggering": "0", "PRIME-Y-dimension": "1200", "Frame": 0, "555 Shutter-Sequenceable": "1", "PRIME-Timing-ExposureTimeNs": "499998000.0000", "Core-ImageProcessor": "", "528-State": "0", "520 AO-Block voltage": "false", "XLIGHT Excitation Wheel-Label": "QUAD", "640 Shutter-Demo": "No", "COM3-StopBits": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "520 Shutter-IOChannel": "Dev1/ao11", "Width": 1200, "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-SupportsTriggering": "0", "640 AO-TriggerSampleRate": "100000", "520-Description": "DA device that is used as a shutter", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "405 Shutter-Sequenceable": "1", "405 Shutter-SupportsTriggering": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-TriggerSampleRate": "100000", "640 Shutter-MaxVolts": "5.0000", "555 AO-MaxVolts": "5.0000", "520 AO-TriggerSequenceLength": "1000", "COM3-BaudRate": "9600", "COM9-BaudRate": "9600", "Vincent-D3-Channel #": "Ch. #1", "PRIME-PMode": "Normal", "OpticalPath-Label": "Left(100)", "BitDepth": 16, "555 AO-TriggerSequenceLength": "1000", "445 Shutter-Description": "NI DAC", "405 AO-Sequenceable": "1", "PRIME-AcquisitionMethod": "Callbacks", "555 AO-Name": "AnalogIO", "PRIME-ShutterMode": "Pre-Exposure", "COM3-Handshaking": "Off", "445 AO-SupportsTriggering": "0", "ROI": "0-0-1200-1200", "COM9-Verbose": "1", "PRIME-Color": "OFF", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "PRIME-ShutterOpenDelay": "0", "COM1-Parity": "None", "Slice": 3, "SlicePosition": -20, "COM9-DelayBetweenCharsMs": "0.0000", "555-Name": "DA Shutter", "PIEZO-LudlSingleAxisName": "B", "XLIGHT Emission Wheel-Label": "725/40 (C)", "640 Shutter-Name": "AnalogIO", "640 AO-Sequenceable": "1", "520 Shutter-Demo": "No", "528 Shutter-Demo": "No", "470-Name": "DA Shutter", "520-DA Device": "520 Shutter", "XYStage-Description": "Ludl XY stage driver adapter", "445 Shutter-MinVolts": "0.0000", "528 AO-TriggerSampleRate": "100000", "405 AO-MinVolts": "0.0000", "405 Shutter-TriggerSequenceLength": "1000", "Height": 1200, "555 AO-Block voltage": "false", "640 Shutter-Volts": "5.0000", "PixelType": "GRAY16", "445 Shutter-Block voltage": "false", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "520 Shutter-TriggerSequenceLength": "1000", "COM9-Name": "COM9", "Core-SLM": "", "555 AO-Volts": "0.0000", "555 Shutter-Block voltage": "false", "XYStage-Speed": "1800.0000", "520 AO-MaxVolts": "5.0000", "528 Shutter-SupportsTriggering": "0", "COM1-Description": "Serial port driver (boost:asio)", "PRIME-MetadataEnabled": "No", "520 Shutter-MinVolts": "0.0000", "470 Shutter-Sequenceable": "1", "PRIME-PreampDelay": "0", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-MinVolts": "0.0000", "ChannelIndex": 3, "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Core-AutoShutter": "1", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "Exposure-ms": 520, "PRIME-PP 2 ENABLED": "Yes", "640 Shutter-TriggerSampleRate": "100000", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "470 Shutter-IOChannel": "Dev1/ao10", "405 AO-Demo": "No", "PixelSizeUm": 1.1, "470 AO-Demo": "No", "470 Shutter-MaxVolts": "5.0000", "Vincent-D3-Name": "Vincent-D3", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FileName": "img_000000000_Il-C_003.tif", "LudlController-Reset": "Operate", "PRIME-Exposure": "520.00", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "445-Name": "DA Shutter", "555 Shutter-Name": "AnalogIO", "520 Shutter-TriggerSampleRate": "100000", "445 Shutter-IOChannel": "Dev1/ao9", "PRIME-TransposeCorrection": "0", "XYStage-StepSize": "0.1000", "445-Description": "DA device that is used as a shutter", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 AO-IOChannel": "Dev1/ao7", "520 Shutter-Name": "AnalogIO", "COM3-DataBits": "8", "640 AO-Volts": "4.5000", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "555 Shutter-IOChannel": "Dev1/ao13", "WaitInterval": null, "520 Shutter-Sequenceable": "1", "YPositionUm": -180, "445-State": "0", "445-DA Device": "445 Shutter", "PRIME-CircularBufferAutoSize": "ON", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "555 AO-TriggerSampleRate": "100000", "445 Shutter-Volts": "0.0000", "XLIGHT Hub-Port": "COM3", "Core-AutoFocus": "", "COM4-Handshaking": "Off", "528 Shutter-MinVolts": "0.0000", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "445 Shutter-Name": "AnalogIO", "528 Shutter-Sequenceable": "1", "XYStage-TransposeMirrorX": "0", "XYStage-TransposeMirrorY": "0", "PRIME-ReadoutRate": "100MHz 16bit", "XYStage-Name": "Stage", "640 AO-SupportsTriggering": "0", "640-Name": "DA Shutter", "640 Shutter-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "640 AO-Block voltage": "false", "445 Shutter-Sequenceable": "1", "470 AO-MaxVolts": "5.0000", "COM4-BaudRate": "9600", "470-Description": "DA device that is used as a shutter", "520 Shutter-Description": "NI DAC", "PRIME-CCDTemperature": "-14.8500", "Time": "2019-12-18 23:11:12 -0500", "640 AO-TriggerSequenceLength": "1000", "470 AO-Name": "AnalogIO", "XLIGHT Disk slider-Label": "Disk Pos 40um", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "Binning": "1x1", "TE2000-ModelType": "1", "COM3-AnswerTimeout": "500.0000", "555 Shutter-SupportsTriggering": "0", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "TE2000-Port": "COM4", "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Description": "NI DAC", "COM4-AnswerTimeout": "500.0000", "COM1-DelayBetweenCharsMs": "0.0000", "FilterBlock-Label": "Confocal", "445 AO-Name": "AnalogIO", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-Timing-ReadoutTimeNs": "24672000.0000", "528 AO-Demo": "No", "520 AO-Sequenceable": "1", "555-Description": "DA device that is used as a shutter", "405-DA Device": "405 Shutter", "405 AO-TriggerSequenceLength": "1000", "640 AO-MinVolts": "0.0000", "640 Shutter-Block voltage": "false", "FrameIndex": 0, "PRIME-Port": "CMOS", "555 AO-MinVolts": "0.0000", "COM3-Description": "Serial port driver (boost:asio)", "528 AO-Description": "NI DAC", "555 AO-IOChannel": "Dev1/ao6", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "555 AO-TriggerInputLine": "/Dev1/PFI0", "PRIME-ActualInterval-ms": "390.5200", "LudlController-Port": "COM9", "445 Shutter-Demo": "No", "PRIME-Trigger-Expose Out-Mux": "1", "SliceIndex": 3, "PRIME-CCDTemperatureSetPoint": "-15.0000", "405 Shutter-MinVolts": "0.0000", "PRIME-ExposeOutMode": "First Row", "AxisPositions": null, "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Volts": "0.0000", "528-Name": "DA Shutter", "555-State": "0", "XLIGHT Dichroic-Label": "Di03-R635-t1", "445 AO-Sequenceable": "1", "405-Name": "DA Shutter", "405 AO-IOChannel": "Dev1/ao0", "Core-Camera": "PRIME", "Focus-Name": "Focus", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "405 Shutter-IOChannel": "Dev1/ao8", "640 AO-Demo": "No", "640 Shutter-SupportsTriggering": "0", "445 AO-Demo": "No", "555-DA Device": "555 Shutter", "470 Shutter-Description": "NI DAC", "528 AO-Volts": "0.0000", "COM1-DataBits": "8", "Vincent-D3-Address": "x", "520 AO-SupportsTriggering": "0", "405-Description": "DA device that is used as a shutter", "640 Shutter-MinVolts": "0.0000", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "405 AO-Name": "AnalogIO", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "640 AO-TriggerInputLine": "/Dev1/PFI0", "405 AO-TriggerInputLine": "/Dev1/PFI0", "640-Description": "DA device that is used as a shutter", "445 Shutter-TriggerSampleRate": "100000", "470 Shutter-TriggerSequenceLength": "1000", "COM1-StopBits": "1", "520 Shutter-SupportsTriggering": "0", "405-State": "0", "Core-Initialize": "1", "XLIGHT Emission Wheel-State": "3", "520 AO-Description": "NI DAC", "528 AO-TriggerInputLine": "/Dev1/PFI0", "445 Shutter-MaxVolts": "5.0000", "COM9-Handshaking": "Off", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "XLIGHT Spin motor-Label": "On (spinning)", "PRIME-PP 5 ENABLED": "No", "UUID": "fdb4c9e4-a08e-4c59-b55f-4757c44aa9f6", "PRIME-TriggerMode": "Internal Trigger", "COM1-Handshaking": "Off", "PRIME-Timing-PostTriggerDelayNs": "49344000.0000", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "Focus-Position": "17741.5000", "528 Shutter-Block voltage": "false", "Channel": "Il-C", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "555 Shutter-Demo": "No", "405 Shutter-Demo": "No", "528 AO-Name": "AnalogIO", "520-State": "0", "Core-Galvo": "", "405 Shutter-TriggerSampleRate": "100000", "Focus-FocusStepSize_(nm)": "100", "PRIME-PixelType": "16bit", "COM4-DataBits": "8", "470 AO-IOChannel": "Dev1/ao2", "520 AO-MinVolts": "0.0000", "COM9-Parity": "None", "PRIME-CircularBufferEnabled": "ON", "PRIME-PP 6 Reset": "No", "640 AO-Description": "NI DAC", "Source": "PRIME", "445 AO-MinVolts": "0.0000", "PIEZO-Name": "Stage", "445 AO-Description": "NI DAC", "PRIME-CircularBufferFrameCount": "46", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "520 AO-Volts": "0.0000", "528 Shutter-TriggerSampleRate": "100000", "XPositionUm": 15712.1, "OpticalPath-State": "4", "PRIME-PP 3 THRESHOLD": "80", "PRIME-PVCAM Version": "3.7.1", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Gain": "1-HDR", "640 Shutter-TriggerSequenceLength": "1000", "528 AO-SupportsTriggering": "0", "445 AO-TriggerInputLine": "/Dev1/PFI0", "OpticalPath-Name": "OpticalPath", "PRIME-PP 4 ENABLED": "Yes", "PRIME-PVCAM Adapter Version": "1.3.29", "COM3-Name": "COM3", "405 AO-MaxVolts": "5.0000", "520 Shutter-Block voltage": "false", "PRIME-Timing-ClearingTimeNs": "49344000.0000", "Core-Focus": "PIEZO", "528 AO-Block voltage": "false", "TE2000-Description": "Nikon TE2000 hub", "528 AO-MinVolts": "0.0000", "COM4-Parity": "None", "Summary": { "Slices": 11, "ChContrastMin": [ 0, 0, 0, 0, 0, 0 ], "PixelAspect": 1, "SlicesFirst": true, "Interval_ms": 0, "Time": "2019-12-18 23:10:50 -0500", "Prefix": "seq_5", "InitialPositionList": [ { "Label": "Pos0", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.21], "Focus": [17741.5], "XYStage": [ 44542.200000000004, 31636.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos1", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.22], "Focus": [17741.4], "XYStage": [ 43801.100000000006, 31637.9 ] }, "GridColumnIndex": 0 }, { "Label": "Pos2", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.5], "XYStage": [ 38561.3, 32040.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos3", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.185], "Focus": [17741.3], "XYStage": [ 37698.4, 32041.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos4", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.16], "Focus": [17741.5], "XYStage": [ 32375.4, 31758.9 ] }, "GridColumnIndex": 0 }, { "Label": "Pos5", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.17000000000002], "Focus": [17741.3], "XYStage": [ 31770.7, 31758.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos6", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.4], "XYStage": [ 26536.800000000003, 31068.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos7", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.205], "Focus": [17741.4], "XYStage": [ 25420.2, 31068.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos8", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.85], "Focus": [17741.3], "XYStage": [ 21059, 31508 ] }, "GridColumnIndex": 0 }, { "Label": "Pos9", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.82], "Focus": [17741.3], "XYStage": [ 19731.9, 31506.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos10", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.79], "Focus": [17741.4], "XYStage": [ 14528.5, 32007.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos11", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.83], "Focus": [17741.2], "XYStage": [ 13830.300000000001, 32009.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos12", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.79], "Focus": [17741.3], "XYStage": [ 8775.300000000001, 32017.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos13", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.815], "Focus": [17741.3], "XYStage": [ 8109.200000000001, 32008.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos14", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.805], "Focus": [17741.3], "XYStage": [ 3483.3, 31908.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos15", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.82], "Focus": [17741.2], "XYStage": [ 2686.8, 31903.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos16", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [211.48000000000002], "Focus": [17741.1], "XYStage": [ -1978.9, 31973.600000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos17", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [211.51500000000001], "Focus": [17741.1], "XYStage": [ -2957.8, 31982.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos18", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.195], "Focus": [17741.5], "XYStage": [ 47747.4, -576.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos19", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.23000000000002], "Focus": [17741.6], "XYStage": [ 47053.9, -576.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos20", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.205], "Focus": [17741.4], "XYStage": [ 41871.200000000004, -479.8 ] }, "GridColumnIndex": 0 }, { "Label": "Pos21", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.4], "XYStage": [ 40882.600000000006, -392 ] }, "GridColumnIndex": 0 }, { "Label": "Pos22", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.24], "Focus": [17741.4], "XYStage": [ 35905.6, -135.3 ] }, "GridColumnIndex": 0 }, { "Label": "Pos23", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.195], "Focus": [17741.5], "XYStage": [ 34990.9, -132.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos24", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.46], "Focus": [17741.4], "XYStage": [ 29678.100000000002, -958.1 ] }, "GridColumnIndex": 0 }, { "Label": "Pos25", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.44], "Focus": [17741.5], "XYStage": [ 28983.600000000002, -939.4000000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos26", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.475], "Focus": [17741.5], "XYStage": [ 23019.7, -122 ] }, "GridColumnIndex": 0 }, { "Label": "Pos27", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.44], "Focus": [17741.7], "XYStage": [ 22428, -116.80000000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos28", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.46], "Focus": [17741.7], "XYStage": [ 16364.900000000001, -181.10000000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos29", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.48000000000002], "Focus": [17741.5], "XYStage": [ 15712.1, -180 ] }, "GridColumnIndex": 0 }, { "Label": "Pos30", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.45000000000002], "Focus": [17741.7], "XYStage": [ 10346.1, 77.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos31", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.49], "Focus": [17741.4], "XYStage": [ 9755.5, 85.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos32", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.475], "Focus": [17741.5], "XYStage": [ 4775.5, 293.90000000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos33", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.445], "Focus": [17741.4], "XYStage": [ 3954.9, 293.90000000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos34", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.455], "Focus": [17741.4], "XYStage": [ -1324.9, 545.6 ] }, "GridColumnIndex": 0 }, { "Label": "Pos35", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.445], "Focus": [17741.3], "XYStage": [ -2097, 576.6 ] }, "GridColumnIndex": 0 } ], "z-step_um": 10, "GridRow": 0, "BitDepth": 16, "Depth": 2, "Channels": 6, "Source": "Micro-Manager", "ChColors": [ -16711681, -256, -65281, -1, -1, -16776961 ], "MetadataVersion": 10, "KeepShutterOpenChannels": true, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536, 65536 ], "CustomIntervals_ms": [], "Height": 1200, "MicroManagerVersion": "1.4.23 20180219", "GridColumn": 0, "UUID": "67598d21-5db5-4842-ab60-f506f7b42c7c", "PixelType": "GRAY16", "Width": 1200, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\Yan\\20191218-YC113-S7S8-miseq", "Frames": 1, "Comment": "", "UserName": "Nikon", "PixelSize_um": 1.1, "ROI": [ 0, 0, 1200, 1200 ], "Date": "2019-12-18", "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC", "P-DAPI-high" ], "KeepShutterOpenSlices": true, "IJType": 1, "ComputerName": "ZadorConfocal2", "Positions": 36, "PositionIndex": 29, "CameraTimeout": 20000, "TimeFirst": false }, "640 AO-Name": "AnalogIO", "Vincent-D3-Command": "Close" }, "FrameKey-0-3-4": { "COM4-StopBits": "1", "528 AO-MaxVolts": "5.0000", "XLIGHT Excitation Wheel-State": "1", "470 Shutter-Name": "AnalogIO", "Core-Shutter": "640", "555 AO-Demo": "No", "XLIGHT Dichroic-State": "2", "XYStage-StepSize-Y": "0.1000", "ZPositionUm": 208.48000000000002, "XYStage-StepSize-X": "0.1000", "PRIME-FirmwareVersion": "20.28", "PRIME-ClearCycles": "2", "520-Name": "DA Shutter", "405 Shutter-Name": "AnalogIO", "PIEZO-StepSize": "0.0050", "PRIME-ClearMode": "Pre-Exposure", "470 AO-MinVolts": "0.0000", "405 AO-SupportsTriggering": "1", "CameraChannelIndex": 0, "XYStage-StartSpeed": "500.0000", "COM9-StopBits": "2", "405 AO-Description": "NI DAC", "COM4-DelayBetweenCharsMs": "0.0000", "445 AO-Block voltage": "false", "520 AO-TriggerInputLine": "/Dev1/PFI0", "555 AO-Description": "NI DAC", "520 AO-TriggerSampleRate": "100000", "COM4-Verbose": "1", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM4-Name": "COM4", "405 Shutter-Volts": "0.0000", "Vincent-D3-Port": "COM1", "PRIME-PP 4 MIN ADU AFFECTED": "200", "470 Shutter-MinVolts": "0.0000", "PRIME-PP 3 ENABLED": "Yes", "470 AO-Description": "NI DAC", "PRIME-Description": "PVCAM API device adapter", "520 Shutter-MaxVolts": "5.0000", "520 Shutter-Volts": "0.0000", "528 AO-Sequenceable": "1", "PRIME-Binning": "1x1", "555 Shutter-MaxVolts": "5.0000", "528 Shutter-TriggerSequenceLength": "1000", "405 Shutter-Block voltage": "false", "XLIGHT Spin motor-State": "1", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "Vincent-D3-Time to open (ms)": "35.0000", "Vincent-D3-Time to close (ms)": "35.0000", "445 AO-TriggerSampleRate": "100000", "640 Shutter-IOChannel": "Dev1/ao14", "555 AO-Sequenceable": "1", "520 AO-Name": "AnalogIO", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "470 AO-Block voltage": "false", "COM1-Verbose": "1", "COM9-AnswerTimeout": "10000.0000", "528 Shutter-Name": "AnalogIO", "PRIME-Trigger Timeout (secs)": "10", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "NextFrame": 0, "470 AO-TriggerSequenceLength": "1000", "PRIME-TransposeXY": "0", "445 AO-TriggerSequenceLength": "1000", "COM1-Name": "COM1", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "COM9-DataBits": "8", "COM1-AnswerTimeout": "500.0000", "Camera": "", "TE2000-Version": "V2.50-V2.50-V2.10", "528 Shutter-Volts": "0.0000", "XLIGHT Hub-Name": "XLIGHT Hub", "PIEZO-Description": "Ludl stage driver adapter", "PositionName": "Pos29", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSampleRate": "100000", "PRIME-FanSpeedSetpoint": "High", "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "405 AO-TriggerSampleRate": "100000", "470 Shutter-Block voltage": "false", "470-State": "0", "COM3-Parity": "None", "405 AO-Volts": "0.0000", "PRIME-TransposeMirrorY": "0", "PRIME-ChipName": "GS144BSI", "470 AO-Volts": "0.0000", "PRIME-TransposeMirrorX": "0", "445 Shutter-SupportsTriggering": "0", "470 Shutter-SupportsTriggering": "0", "XLIGHT Hub-Description": "XLIGHT hub", "PRIME-PreampOffLimit": "10000", "470 AO-Sequenceable": "1", "528 Shutter-MaxVolts": "5.0000", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "Core-XYStage": "XYStage", "470 AO-TriggerSampleRate": "100000", "PRIME-Timing-PreTriggerDelayNs": "24672000.0000", "528-DA Device": "528 Shutter", "Focus-Description": "Nikon TE2000 focus adapter", "FilterBlock-State": "5", "520 AO-Demo": "No", "COM3-DelayBetweenCharsMs": "0.0000", "470 Shutter-Volts": "0.0000", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "PRIME-PP 2 THRESHOLD": "125", "PRIME-PP 4 THRESHOLD": "75", "XYStage-Acceleration": "4.0000", "470 Shutter-Demo": "No", "PRIME-Name": "PMPCIECam00", "640-DA Device": "640 Shutter", "405 AO-Block voltage": "false", "ElapsedTime-ms": 1212686, "PRIME-Offset": "100", "445 AO-IOChannel": "Dev1/ao1", "640 AO-MaxVolts": "5.0000", "PositionIndex": 29, "PRIME-PP 1 ENABLED": "Yes", "COM1-BaudRate": "9600", "640-State": "1", "COM3-Verbose": "1", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "LudlController-TransmissionDelay": "0", "PRIME-FullWellCapacity": "62000", "PRIME-SMARTStreamingEnabled": "No", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "640 Shutter-Description": "NI DAC", "528-Description": "DA device that is used as a shutter", "COM4-Description": "Serial port driver (boost:asio)", "405 Shutter-Description": "NI DAC", "TE2000-Name": "TE2000", "528 Shutter-Description": "NI DAC", "COM9-Description": "Serial port driver (boost:asio)", "LudlController-Name": "LudlController", "445 Shutter-TriggerSequenceLength": "1000", "PRIME-SerialNumber": "A18B203008", "445 AO-Volts": "0.0000", "PRIME-X-dimension": "1200", "PRIME-ShutterCloseDelay": "0", "XLIGHT Disk slider-State": "2", "Position": "Default", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 AO-IOChannel": "Dev1/ao5", "520 AO-IOChannel": "Dev1/ao4", "528 AO-TriggerSequenceLength": "1000", "405 Shutter-MaxVolts": "5.0000", "470-DA Device": "470 Shutter", "PRIME-FTCapable": "No", "Core-ChannelGroup": "Channels", "555 AO-SupportsTriggering": "0", "PRIME-Y-dimension": "1200", "Frame": 0, "555 Shutter-Sequenceable": "1", "PRIME-Timing-ExposureTimeNs": "499998000.0000", "Core-ImageProcessor": "", "528-State": "0", "520 AO-Block voltage": "false", "XLIGHT Excitation Wheel-Label": "QUAD", "640 Shutter-Demo": "No", "COM3-StopBits": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "520 Shutter-IOChannel": "Dev1/ao11", "Width": 1200, "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-SupportsTriggering": "0", "640 AO-TriggerSampleRate": "100000", "520-Description": "DA device that is used as a shutter", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "405 Shutter-Sequenceable": "1", "405 Shutter-SupportsTriggering": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-TriggerSampleRate": "100000", "640 Shutter-MaxVolts": "5.0000", "555 AO-MaxVolts": "5.0000", "520 AO-TriggerSequenceLength": "1000", "COM3-BaudRate": "9600", "COM9-BaudRate": "9600", "Vincent-D3-Channel #": "Ch. #1", "PRIME-PMode": "Normal", "OpticalPath-Label": "Left(100)", "BitDepth": 16, "555 AO-TriggerSequenceLength": "1000", "445 Shutter-Description": "NI DAC", "405 AO-Sequenceable": "1", "PRIME-AcquisitionMethod": "Callbacks", "555 AO-Name": "AnalogIO", "PRIME-ShutterMode": "Pre-Exposure", "COM3-Handshaking": "Off", "445 AO-SupportsTriggering": "0", "ROI": "0-0-1200-1200", "COM9-Verbose": "1", "PRIME-Color": "OFF", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "PRIME-ShutterOpenDelay": "0", "COM1-Parity": "None", "Slice": 4, "SlicePosition": -10, "COM9-DelayBetweenCharsMs": "0.0000", "555-Name": "DA Shutter", "PIEZO-LudlSingleAxisName": "B", "XLIGHT Emission Wheel-Label": "725/40 (C)", "640 Shutter-Name": "AnalogIO", "640 AO-Sequenceable": "1", "520 Shutter-Demo": "No", "528 Shutter-Demo": "No", "470-Name": "DA Shutter", "520-DA Device": "520 Shutter", "XYStage-Description": "Ludl XY stage driver adapter", "445 Shutter-MinVolts": "0.0000", "528 AO-TriggerSampleRate": "100000", "405 AO-MinVolts": "0.0000", "405 Shutter-TriggerSequenceLength": "1000", "Height": 1200, "555 AO-Block voltage": "false", "640 Shutter-Volts": "5.0000", "PixelType": "GRAY16", "445 Shutter-Block voltage": "false", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "520 Shutter-TriggerSequenceLength": "1000", "COM9-Name": "COM9", "Core-SLM": "", "555 AO-Volts": "0.0000", "555 Shutter-Block voltage": "false", "XYStage-Speed": "1800.0000", "520 AO-MaxVolts": "5.0000", "528 Shutter-SupportsTriggering": "0", "COM1-Description": "Serial port driver (boost:asio)", "PRIME-MetadataEnabled": "No", "520 Shutter-MinVolts": "0.0000", "470 Shutter-Sequenceable": "1", "PRIME-PreampDelay": "0", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-MinVolts": "0.0000", "ChannelIndex": 3, "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Core-AutoShutter": "1", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "Exposure-ms": 520, "PRIME-PP 2 ENABLED": "Yes", "640 Shutter-TriggerSampleRate": "100000", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "470 Shutter-IOChannel": "Dev1/ao10", "405 AO-Demo": "No", "PixelSizeUm": 1.1, "470 AO-Demo": "No", "470 Shutter-MaxVolts": "5.0000", "Vincent-D3-Name": "Vincent-D3", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FileName": "img_000000000_Il-C_004.tif", "LudlController-Reset": "Operate", "PRIME-Exposure": "520.00", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "445-Name": "DA Shutter", "555 Shutter-Name": "AnalogIO", "520 Shutter-TriggerSampleRate": "100000", "445 Shutter-IOChannel": "Dev1/ao9", "PRIME-TransposeCorrection": "0", "XYStage-StepSize": "0.1000", "445-Description": "DA device that is used as a shutter", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 AO-IOChannel": "Dev1/ao7", "520 Shutter-Name": "AnalogIO", "COM3-DataBits": "8", "640 AO-Volts": "4.5000", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "555 Shutter-IOChannel": "Dev1/ao13", "WaitInterval": null, "520 Shutter-Sequenceable": "1", "YPositionUm": -180, "445-State": "0", "445-DA Device": "445 Shutter", "PRIME-CircularBufferAutoSize": "ON", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "555 AO-TriggerSampleRate": "100000", "445 Shutter-Volts": "0.0000", "XLIGHT Hub-Port": "COM3", "Core-AutoFocus": "", "COM4-Handshaking": "Off", "528 Shutter-MinVolts": "0.0000", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "445 Shutter-Name": "AnalogIO", "528 Shutter-Sequenceable": "1", "XYStage-TransposeMirrorX": "0", "XYStage-TransposeMirrorY": "0", "PRIME-ReadoutRate": "100MHz 16bit", "XYStage-Name": "Stage", "640 AO-SupportsTriggering": "0", "640-Name": "DA Shutter", "640 Shutter-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "640 AO-Block voltage": "false", "445 Shutter-Sequenceable": "1", "470 AO-MaxVolts": "5.0000", "COM4-BaudRate": "9600", "470-Description": "DA device that is used as a shutter", "520 Shutter-Description": "NI DAC", "PRIME-CCDTemperature": "-14.8500", "Time": "2019-12-18 23:11:12 -0500", "640 AO-TriggerSequenceLength": "1000", "470 AO-Name": "AnalogIO", "XLIGHT Disk slider-Label": "Disk Pos 40um", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "Binning": "1x1", "TE2000-ModelType": "1", "COM3-AnswerTimeout": "500.0000", "555 Shutter-SupportsTriggering": "0", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "TE2000-Port": "COM4", "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Description": "NI DAC", "COM4-AnswerTimeout": "500.0000", "COM1-DelayBetweenCharsMs": "0.0000", "FilterBlock-Label": "Confocal", "445 AO-Name": "AnalogIO", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-Timing-ReadoutTimeNs": "24672000.0000", "528 AO-Demo": "No", "520 AO-Sequenceable": "1", "555-Description": "DA device that is used as a shutter", "405-DA Device": "405 Shutter", "405 AO-TriggerSequenceLength": "1000", "640 AO-MinVolts": "0.0000", "640 Shutter-Block voltage": "false", "FrameIndex": 0, "PRIME-Port": "CMOS", "555 AO-MinVolts": "0.0000", "COM3-Description": "Serial port driver (boost:asio)", "528 AO-Description": "NI DAC", "555 AO-IOChannel": "Dev1/ao6", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "555 AO-TriggerInputLine": "/Dev1/PFI0", "PRIME-ActualInterval-ms": "390.5200", "LudlController-Port": "COM9", "445 Shutter-Demo": "No", "PRIME-Trigger-Expose Out-Mux": "1", "SliceIndex": 4, "PRIME-CCDTemperatureSetPoint": "-15.0000", "405 Shutter-MinVolts": "0.0000", "PRIME-ExposeOutMode": "First Row", "AxisPositions": null, "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Volts": "0.0000", "528-Name": "DA Shutter", "555-State": "0", "XLIGHT Dichroic-Label": "Di03-R635-t1", "445 AO-Sequenceable": "1", "405-Name": "DA Shutter", "405 AO-IOChannel": "Dev1/ao0", "Core-Camera": "PRIME", "Focus-Name": "Focus", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "405 Shutter-IOChannel": "Dev1/ao8", "640 AO-Demo": "No", "640 Shutter-SupportsTriggering": "0", "445 AO-Demo": "No", "555-DA Device": "555 Shutter", "470 Shutter-Description": "NI DAC", "528 AO-Volts": "0.0000", "COM1-DataBits": "8", "Vincent-D3-Address": "x", "520 AO-SupportsTriggering": "0", "405-Description": "DA device that is used as a shutter", "640 Shutter-MinVolts": "0.0000", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "405 AO-Name": "AnalogIO", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "640 AO-TriggerInputLine": "/Dev1/PFI0", "405 AO-TriggerInputLine": "/Dev1/PFI0", "640-Description": "DA device that is used as a shutter", "445 Shutter-TriggerSampleRate": "100000", "470 Shutter-TriggerSequenceLength": "1000", "COM1-StopBits": "1", "520 Shutter-SupportsTriggering": "0", "405-State": "0", "Core-Initialize": "1", "XLIGHT Emission Wheel-State": "3", "520 AO-Description": "NI DAC", "528 AO-TriggerInputLine": "/Dev1/PFI0", "445 Shutter-MaxVolts": "5.0000", "COM9-Handshaking": "Off", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "XLIGHT Spin motor-Label": "On (spinning)", "PRIME-PP 5 ENABLED": "No", "UUID": "d87548a3-d97f-4bfa-965b-02985576f52c", "PRIME-TriggerMode": "Internal Trigger", "COM1-Handshaking": "Off", "PRIME-Timing-PostTriggerDelayNs": "49344000.0000", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "Focus-Position": "17741.5000", "528 Shutter-Block voltage": "false", "Channel": "Il-C", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "555 Shutter-Demo": "No", "405 Shutter-Demo": "No", "528 AO-Name": "AnalogIO", "520-State": "0", "Core-Galvo": "", "405 Shutter-TriggerSampleRate": "100000", "Focus-FocusStepSize_(nm)": "100", "PRIME-PixelType": "16bit", "COM4-DataBits": "8", "470 AO-IOChannel": "Dev1/ao2", "520 AO-MinVolts": "0.0000", "COM9-Parity": "None", "PRIME-CircularBufferEnabled": "ON", "PRIME-PP 6 Reset": "No", "640 AO-Description": "NI DAC", "Source": "PRIME", "445 AO-MinVolts": "0.0000", "PIEZO-Name": "Stage", "445 AO-Description": "NI DAC", "PRIME-CircularBufferFrameCount": "46", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "520 AO-Volts": "0.0000", "528 Shutter-TriggerSampleRate": "100000", "XPositionUm": 15712.1, "OpticalPath-State": "4", "PRIME-PP 3 THRESHOLD": "80", "PRIME-PVCAM Version": "3.7.1", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Gain": "1-HDR", "640 Shutter-TriggerSequenceLength": "1000", "528 AO-SupportsTriggering": "0", "445 AO-TriggerInputLine": "/Dev1/PFI0", "OpticalPath-Name": "OpticalPath", "PRIME-PP 4 ENABLED": "Yes", "PRIME-PVCAM Adapter Version": "1.3.29", "COM3-Name": "COM3", "405 AO-MaxVolts": "5.0000", "520 Shutter-Block voltage": "false", "PRIME-Timing-ClearingTimeNs": "49344000.0000", "Core-Focus": "PIEZO", "528 AO-Block voltage": "false", "TE2000-Description": "Nikon TE2000 hub", "528 AO-MinVolts": "0.0000", "COM4-Parity": "None", "Summary": { "Slices": 11, "ChContrastMin": [ 0, 0, 0, 0, 0, 0 ], "PixelAspect": 1, "SlicesFirst": true, "Interval_ms": 0, "Time": "2019-12-18 23:10:50 -0500", "Prefix": "seq_5", "InitialPositionList": [ { "Label": "Pos0", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.21], "Focus": [17741.5], "XYStage": [ 44542.200000000004, 31636.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos1", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.22], "Focus": [17741.4], "XYStage": [ 43801.100000000006, 31637.9 ] }, "GridColumnIndex": 0 }, { "Label": "Pos2", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.5], "XYStage": [ 38561.3, 32040.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos3", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.185], "Focus": [17741.3], "XYStage": [ 37698.4, 32041.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos4", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.16], "Focus": [17741.5], "XYStage": [ 32375.4, 31758.9 ] }, "GridColumnIndex": 0 }, { "Label": "Pos5", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.17000000000002], "Focus": [17741.3], "XYStage": [ 31770.7, 31758.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos6", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.4], "XYStage": [ 26536.800000000003, 31068.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos7", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.205], "Focus": [17741.4], "XYStage": [ 25420.2, 31068.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos8", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.85], "Focus": [17741.3], "XYStage": [ 21059, 31508 ] }, "GridColumnIndex": 0 }, { "Label": "Pos9", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.82], "Focus": [17741.3], "XYStage": [ 19731.9, 31506.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos10", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.79], "Focus": [17741.4], "XYStage": [ 14528.5, 32007.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos11", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.83], "Focus": [17741.2], "XYStage": [ 13830.300000000001, 32009.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos12", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.79], "Focus": [17741.3], "XYStage": [ 8775.300000000001, 32017.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos13", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.815], "Focus": [17741.3], "XYStage": [ 8109.200000000001, 32008.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos14", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.805], "Focus": [17741.3], "XYStage": [ 3483.3, 31908.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos15", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.82], "Focus": [17741.2], "XYStage": [ 2686.8, 31903.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos16", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [211.48000000000002], "Focus": [17741.1], "XYStage": [ -1978.9, 31973.600000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos17", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [211.51500000000001], "Focus": [17741.1], "XYStage": [ -2957.8, 31982.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos18", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.195], "Focus": [17741.5], "XYStage": [ 47747.4, -576.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos19", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.23000000000002], "Focus": [17741.6], "XYStage": [ 47053.9, -576.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos20", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.205], "Focus": [17741.4], "XYStage": [ 41871.200000000004, -479.8 ] }, "GridColumnIndex": 0 }, { "Label": "Pos21", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.4], "XYStage": [ 40882.600000000006, -392 ] }, "GridColumnIndex": 0 }, { "Label": "Pos22", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.24], "Focus": [17741.4], "XYStage": [ 35905.6, -135.3 ] }, "GridColumnIndex": 0 }, { "Label": "Pos23", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.195], "Focus": [17741.5], "XYStage": [ 34990.9, -132.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos24", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.46], "Focus": [17741.4], "XYStage": [ 29678.100000000002, -958.1 ] }, "GridColumnIndex": 0 }, { "Label": "Pos25", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.44], "Focus": [17741.5], "XYStage": [ 28983.600000000002, -939.4000000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos26", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.475], "Focus": [17741.5], "XYStage": [ 23019.7, -122 ] }, "GridColumnIndex": 0 }, { "Label": "Pos27", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.44], "Focus": [17741.7], "XYStage": [ 22428, -116.80000000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos28", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.46], "Focus": [17741.7], "XYStage": [ 16364.900000000001, -181.10000000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos29", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.48000000000002], "Focus": [17741.5], "XYStage": [ 15712.1, -180 ] }, "GridColumnIndex": 0 }, { "Label": "Pos30", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.45000000000002], "Focus": [17741.7], "XYStage": [ 10346.1, 77.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos31", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.49], "Focus": [17741.4], "XYStage": [ 9755.5, 85.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos32", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.475], "Focus": [17741.5], "XYStage": [ 4775.5, 293.90000000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos33", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.445], "Focus": [17741.4], "XYStage": [ 3954.9, 293.90000000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos34", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.455], "Focus": [17741.4], "XYStage": [ -1324.9, 545.6 ] }, "GridColumnIndex": 0 }, { "Label": "Pos35", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.445], "Focus": [17741.3], "XYStage": [ -2097, 576.6 ] }, "GridColumnIndex": 0 } ], "z-step_um": 10, "GridRow": 0, "BitDepth": 16, "Depth": 2, "Channels": 6, "Source": "Micro-Manager", "ChColors": [ -16711681, -256, -65281, -1, -1, -16776961 ], "MetadataVersion": 10, "KeepShutterOpenChannels": true, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536, 65536 ], "CustomIntervals_ms": [], "Height": 1200, "MicroManagerVersion": "1.4.23 20180219", "GridColumn": 0, "UUID": "67598d21-5db5-4842-ab60-f506f7b42c7c", "PixelType": "GRAY16", "Width": 1200, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\Yan\\20191218-YC113-S7S8-miseq", "Frames": 1, "Comment": "", "UserName": "Nikon", "PixelSize_um": 1.1, "ROI": [ 0, 0, 1200, 1200 ], "Date": "2019-12-18", "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC", "P-DAPI-high" ], "KeepShutterOpenSlices": true, "IJType": 1, "ComputerName": "ZadorConfocal2", "Positions": 36, "PositionIndex": 29, "CameraTimeout": 20000, "TimeFirst": false }, "640 AO-Name": "AnalogIO", "Vincent-D3-Command": "Close" }, "FrameKey-0-3-5": { "COM4-StopBits": "1", "528 AO-MaxVolts": "5.0000", "XLIGHT Excitation Wheel-State": "1", "470 Shutter-Name": "AnalogIO", "Core-Shutter": "640", "555 AO-Demo": "No", "XLIGHT Dichroic-State": "2", "XYStage-StepSize-Y": "0.1000", "ZPositionUm": 218.48000000000002, "XYStage-StepSize-X": "0.1000", "PRIME-FirmwareVersion": "20.28", "PRIME-ClearCycles": "2", "520-Name": "DA Shutter", "405 Shutter-Name": "AnalogIO", "PIEZO-StepSize": "0.0050", "PRIME-ClearMode": "Pre-Exposure", "470 AO-MinVolts": "0.0000", "405 AO-SupportsTriggering": "1", "CameraChannelIndex": 0, "XYStage-StartSpeed": "500.0000", "COM9-StopBits": "2", "405 AO-Description": "NI DAC", "COM4-DelayBetweenCharsMs": "0.0000", "445 AO-Block voltage": "false", "520 AO-TriggerInputLine": "/Dev1/PFI0", "555 AO-Description": "NI DAC", "520 AO-TriggerSampleRate": "100000", "COM4-Verbose": "1", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM4-Name": "COM4", "405 Shutter-Volts": "0.0000", "Vincent-D3-Port": "COM1", "PRIME-PP 4 MIN ADU AFFECTED": "200", "470 Shutter-MinVolts": "0.0000", "PRIME-PP 3 ENABLED": "Yes", "470 AO-Description": "NI DAC", "PRIME-Description": "PVCAM API device adapter", "520 Shutter-MaxVolts": "5.0000", "520 Shutter-Volts": "0.0000", "528 AO-Sequenceable": "1", "PRIME-Binning": "1x1", "555 Shutter-MaxVolts": "5.0000", "528 Shutter-TriggerSequenceLength": "1000", "405 Shutter-Block voltage": "false", "XLIGHT Spin motor-State": "1", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "Vincent-D3-Time to open (ms)": "35.0000", "Vincent-D3-Time to close (ms)": "35.0000", "445 AO-TriggerSampleRate": "100000", "640 Shutter-IOChannel": "Dev1/ao14", "555 AO-Sequenceable": "1", "520 AO-Name": "AnalogIO", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "470 AO-Block voltage": "false", "COM1-Verbose": "1", "COM9-AnswerTimeout": "10000.0000", "528 Shutter-Name": "AnalogIO", "PRIME-Trigger Timeout (secs)": "10", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "NextFrame": 0, "470 AO-TriggerSequenceLength": "1000", "PRIME-TransposeXY": "0", "445 AO-TriggerSequenceLength": "1000", "COM1-Name": "COM1", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "COM9-DataBits": "8", "COM1-AnswerTimeout": "500.0000", "Camera": "", "TE2000-Version": "V2.50-V2.50-V2.10", "528 Shutter-Volts": "0.0000", "XLIGHT Hub-Name": "XLIGHT Hub", "PIEZO-Description": "Ludl stage driver adapter", "PositionName": "Pos29", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSampleRate": "100000", "PRIME-FanSpeedSetpoint": "High", "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "405 AO-TriggerSampleRate": "100000", "470 Shutter-Block voltage": "false", "470-State": "0", "COM3-Parity": "None", "405 AO-Volts": "0.0000", "PRIME-TransposeMirrorY": "0", "PRIME-ChipName": "GS144BSI", "470 AO-Volts": "0.0000", "PRIME-TransposeMirrorX": "0", "445 Shutter-SupportsTriggering": "0", "470 Shutter-SupportsTriggering": "0", "XLIGHT Hub-Description": "XLIGHT hub", "PRIME-PreampOffLimit": "10000", "470 AO-Sequenceable": "1", "528 Shutter-MaxVolts": "5.0000", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "Core-XYStage": "XYStage", "470 AO-TriggerSampleRate": "100000", "PRIME-Timing-PreTriggerDelayNs": "24672000.0000", "528-DA Device": "528 Shutter", "Focus-Description": "Nikon TE2000 focus adapter", "FilterBlock-State": "5", "520 AO-Demo": "No", "COM3-DelayBetweenCharsMs": "0.0000", "470 Shutter-Volts": "0.0000", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "PRIME-PP 2 THRESHOLD": "125", "PRIME-PP 4 THRESHOLD": "75", "XYStage-Acceleration": "4.0000", "470 Shutter-Demo": "No", "PRIME-Name": "PMPCIECam00", "640-DA Device": "640 Shutter", "405 AO-Block voltage": "false", "ElapsedTime-ms": 1213438, "PRIME-Offset": "100", "445 AO-IOChannel": "Dev1/ao1", "640 AO-MaxVolts": "5.0000", "PositionIndex": 29, "PRIME-PP 1 ENABLED": "Yes", "COM1-BaudRate": "9600", "640-State": "1", "COM3-Verbose": "1", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "LudlController-TransmissionDelay": "0", "PRIME-FullWellCapacity": "62000", "PRIME-SMARTStreamingEnabled": "No", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "640 Shutter-Description": "NI DAC", "528-Description": "DA device that is used as a shutter", "COM4-Description": "Serial port driver (boost:asio)", "405 Shutter-Description": "NI DAC", "TE2000-Name": "TE2000", "528 Shutter-Description": "NI DAC", "COM9-Description": "Serial port driver (boost:asio)", "LudlController-Name": "LudlController", "445 Shutter-TriggerSequenceLength": "1000", "PRIME-SerialNumber": "A18B203008", "445 AO-Volts": "0.0000", "PRIME-X-dimension": "1200", "PRIME-ShutterCloseDelay": "0", "XLIGHT Disk slider-State": "2", "Position": "Default", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 AO-IOChannel": "Dev1/ao5", "520 AO-IOChannel": "Dev1/ao4", "528 AO-TriggerSequenceLength": "1000", "405 Shutter-MaxVolts": "5.0000", "470-DA Device": "470 Shutter", "PRIME-FTCapable": "No", "Core-ChannelGroup": "Channels", "555 AO-SupportsTriggering": "0", "PRIME-Y-dimension": "1200", "Frame": 0, "555 Shutter-Sequenceable": "1", "PRIME-Timing-ExposureTimeNs": "499998000.0000", "Core-ImageProcessor": "", "528-State": "0", "520 AO-Block voltage": "false", "XLIGHT Excitation Wheel-Label": "QUAD", "640 Shutter-Demo": "No", "COM3-StopBits": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "520 Shutter-IOChannel": "Dev1/ao11", "Width": 1200, "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-SupportsTriggering": "0", "640 AO-TriggerSampleRate": "100000", "520-Description": "DA device that is used as a shutter", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "405 Shutter-Sequenceable": "1", "405 Shutter-SupportsTriggering": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-TriggerSampleRate": "100000", "640 Shutter-MaxVolts": "5.0000", "555 AO-MaxVolts": "5.0000", "520 AO-TriggerSequenceLength": "1000", "COM3-BaudRate": "9600", "COM9-BaudRate": "9600", "Vincent-D3-Channel #": "Ch. #1", "PRIME-PMode": "Normal", "OpticalPath-Label": "Left(100)", "BitDepth": 16, "555 AO-TriggerSequenceLength": "1000", "445 Shutter-Description": "NI DAC", "405 AO-Sequenceable": "1", "PRIME-AcquisitionMethod": "Callbacks", "555 AO-Name": "AnalogIO", "PRIME-ShutterMode": "Pre-Exposure", "COM3-Handshaking": "Off", "445 AO-SupportsTriggering": "0", "ROI": "0-0-1200-1200", "COM9-Verbose": "1", "PRIME-Color": "OFF", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "PRIME-ShutterOpenDelay": "0", "COM1-Parity": "None", "Slice": 5, "SlicePosition": 0, "COM9-DelayBetweenCharsMs": "0.0000", "555-Name": "DA Shutter", "PIEZO-LudlSingleAxisName": "B", "XLIGHT Emission Wheel-Label": "725/40 (C)", "640 Shutter-Name": "AnalogIO", "640 AO-Sequenceable": "1", "520 Shutter-Demo": "No", "528 Shutter-Demo": "No", "470-Name": "DA Shutter", "520-DA Device": "520 Shutter", "XYStage-Description": "Ludl XY stage driver adapter", "445 Shutter-MinVolts": "0.0000", "528 AO-TriggerSampleRate": "100000", "405 AO-MinVolts": "0.0000", "405 Shutter-TriggerSequenceLength": "1000", "Height": 1200, "555 AO-Block voltage": "false", "640 Shutter-Volts": "5.0000", "PixelType": "GRAY16", "445 Shutter-Block voltage": "false", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "520 Shutter-TriggerSequenceLength": "1000", "COM9-Name": "COM9", "Core-SLM": "", "555 AO-Volts": "0.0000", "555 Shutter-Block voltage": "false", "XYStage-Speed": "1800.0000", "520 AO-MaxVolts": "5.0000", "528 Shutter-SupportsTriggering": "0", "COM1-Description": "Serial port driver (boost:asio)", "PRIME-MetadataEnabled": "No", "520 Shutter-MinVolts": "0.0000", "470 Shutter-Sequenceable": "1", "PRIME-PreampDelay": "0", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-MinVolts": "0.0000", "ChannelIndex": 3, "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Core-AutoShutter": "1", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "Exposure-ms": 520, "PRIME-PP 2 ENABLED": "Yes", "640 Shutter-TriggerSampleRate": "100000", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "470 Shutter-IOChannel": "Dev1/ao10", "405 AO-Demo": "No", "PixelSizeUm": 1.1, "470 AO-Demo": "No", "470 Shutter-MaxVolts": "5.0000", "Vincent-D3-Name": "Vincent-D3", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FileName": "img_000000000_Il-C_005.tif", "LudlController-Reset": "Operate", "PRIME-Exposure": "520.00", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "445-Name": "DA Shutter", "555 Shutter-Name": "AnalogIO", "520 Shutter-TriggerSampleRate": "100000", "445 Shutter-IOChannel": "Dev1/ao9", "PRIME-TransposeCorrection": "0", "XYStage-StepSize": "0.1000", "445-Description": "DA device that is used as a shutter", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 AO-IOChannel": "Dev1/ao7", "520 Shutter-Name": "AnalogIO", "COM3-DataBits": "8", "640 AO-Volts": "4.5000", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "555 Shutter-IOChannel": "Dev1/ao13", "WaitInterval": null, "520 Shutter-Sequenceable": "1", "YPositionUm": -180, "445-State": "0", "445-DA Device": "445 Shutter", "PRIME-CircularBufferAutoSize": "ON", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "555 AO-TriggerSampleRate": "100000", "445 Shutter-Volts": "0.0000", "XLIGHT Hub-Port": "COM3", "Core-AutoFocus": "", "COM4-Handshaking": "Off", "528 Shutter-MinVolts": "0.0000", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "445 Shutter-Name": "AnalogIO", "528 Shutter-Sequenceable": "1", "XYStage-TransposeMirrorX": "0", "XYStage-TransposeMirrorY": "0", "PRIME-ReadoutRate": "100MHz 16bit", "XYStage-Name": "Stage", "640 AO-SupportsTriggering": "0", "640-Name": "DA Shutter", "640 Shutter-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "640 AO-Block voltage": "false", "445 Shutter-Sequenceable": "1", "470 AO-MaxVolts": "5.0000", "COM4-BaudRate": "9600", "470-Description": "DA device that is used as a shutter", "520 Shutter-Description": "NI DAC", "PRIME-CCDTemperature": "-14.8500", "Time": "2019-12-18 23:11:13 -0500", "640 AO-TriggerSequenceLength": "1000", "470 AO-Name": "AnalogIO", "XLIGHT Disk slider-Label": "Disk Pos 40um", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "Binning": "1x1", "TE2000-ModelType": "1", "COM3-AnswerTimeout": "500.0000", "555 Shutter-SupportsTriggering": "0", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "TE2000-Port": "COM4", "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Description": "NI DAC", "COM4-AnswerTimeout": "500.0000", "COM1-DelayBetweenCharsMs": "0.0000", "FilterBlock-Label": "Confocal", "445 AO-Name": "AnalogIO", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-Timing-ReadoutTimeNs": "24672000.0000", "528 AO-Demo": "No", "520 AO-Sequenceable": "1", "555-Description": "DA device that is used as a shutter", "405-DA Device": "405 Shutter", "405 AO-TriggerSequenceLength": "1000", "640 AO-MinVolts": "0.0000", "640 Shutter-Block voltage": "false", "FrameIndex": 0, "PRIME-Port": "CMOS", "555 AO-MinVolts": "0.0000", "COM3-Description": "Serial port driver (boost:asio)", "528 AO-Description": "NI DAC", "555 AO-IOChannel": "Dev1/ao6", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "555 AO-TriggerInputLine": "/Dev1/PFI0", "PRIME-ActualInterval-ms": "390.5200", "LudlController-Port": "COM9", "445 Shutter-Demo": "No", "PRIME-Trigger-Expose Out-Mux": "1", "SliceIndex": 5, "PRIME-CCDTemperatureSetPoint": "-15.0000", "405 Shutter-MinVolts": "0.0000", "PRIME-ExposeOutMode": "First Row", "AxisPositions": null, "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Volts": "0.0000", "528-Name": "DA Shutter", "555-State": "0", "XLIGHT Dichroic-Label": "Di03-R635-t1", "445 AO-Sequenceable": "1", "405-Name": "DA Shutter", "405 AO-IOChannel": "Dev1/ao0", "Core-Camera": "PRIME", "Focus-Name": "Focus", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "405 Shutter-IOChannel": "Dev1/ao8", "640 AO-Demo": "No", "640 Shutter-SupportsTriggering": "0", "445 AO-Demo": "No", "555-DA Device": "555 Shutter", "470 Shutter-Description": "NI DAC", "528 AO-Volts": "0.0000", "COM1-DataBits": "8", "Vincent-D3-Address": "x", "520 AO-SupportsTriggering": "0", "405-Description": "DA device that is used as a shutter", "640 Shutter-MinVolts": "0.0000", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "405 AO-Name": "AnalogIO", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "640 AO-TriggerInputLine": "/Dev1/PFI0", "405 AO-TriggerInputLine": "/Dev1/PFI0", "640-Description": "DA device that is used as a shutter", "445 Shutter-TriggerSampleRate": "100000", "470 Shutter-TriggerSequenceLength": "1000", "COM1-StopBits": "1", "520 Shutter-SupportsTriggering": "0", "405-State": "0", "Core-Initialize": "1", "XLIGHT Emission Wheel-State": "3", "520 AO-Description": "NI DAC", "528 AO-TriggerInputLine": "/Dev1/PFI0", "445 Shutter-MaxVolts": "5.0000", "COM9-Handshaking": "Off", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "XLIGHT Spin motor-Label": "On (spinning)", "PRIME-PP 5 ENABLED": "No", "UUID": "9a68b1fd-d8c5-4a44-8a9d-f5e88d149d61", "PRIME-TriggerMode": "Internal Trigger", "COM1-Handshaking": "Off", "PRIME-Timing-PostTriggerDelayNs": "49344000.0000", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "Focus-Position": "17741.5000", "528 Shutter-Block voltage": "false", "Channel": "Il-C", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "555 Shutter-Demo": "No", "405 Shutter-Demo": "No", "528 AO-Name": "AnalogIO", "520-State": "0", "Core-Galvo": "", "405 Shutter-TriggerSampleRate": "100000", "Focus-FocusStepSize_(nm)": "100", "PRIME-PixelType": "16bit", "COM4-DataBits": "8", "470 AO-IOChannel": "Dev1/ao2", "520 AO-MinVolts": "0.0000", "COM9-Parity": "None", "PRIME-CircularBufferEnabled": "ON", "PRIME-PP 6 Reset": "No", "640 AO-Description": "NI DAC", "Source": "PRIME", "445 AO-MinVolts": "0.0000", "PIEZO-Name": "Stage", "445 AO-Description": "NI DAC", "PRIME-CircularBufferFrameCount": "46", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "520 AO-Volts": "0.0000", "528 Shutter-TriggerSampleRate": "100000", "XPositionUm": 15712.1, "OpticalPath-State": "4", "PRIME-PP 3 THRESHOLD": "80", "PRIME-PVCAM Version": "3.7.1", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Gain": "1-HDR", "640 Shutter-TriggerSequenceLength": "1000", "528 AO-SupportsTriggering": "0", "445 AO-TriggerInputLine": "/Dev1/PFI0", "OpticalPath-Name": "OpticalPath", "PRIME-PP 4 ENABLED": "Yes", "PRIME-PVCAM Adapter Version": "1.3.29", "COM3-Name": "COM3", "405 AO-MaxVolts": "5.0000", "520 Shutter-Block voltage": "false", "PRIME-Timing-ClearingTimeNs": "49344000.0000", "Core-Focus": "PIEZO", "528 AO-Block voltage": "false", "TE2000-Description": "Nikon TE2000 hub", "528 AO-MinVolts": "0.0000", "COM4-Parity": "None", "Summary": { "Slices": 11, "ChContrastMin": [ 0, 0, 0, 0, 0, 0 ], "PixelAspect": 1, "SlicesFirst": true, "Interval_ms": 0, "Time": "2019-12-18 23:10:50 -0500", "Prefix": "seq_5", "InitialPositionList": [ { "Label": "Pos0", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.21], "Focus": [17741.5], "XYStage": [ 44542.200000000004, 31636.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos1", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.22], "Focus": [17741.4], "XYStage": [ 43801.100000000006, 31637.9 ] }, "GridColumnIndex": 0 }, { "Label": "Pos2", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.5], "XYStage": [ 38561.3, 32040.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos3", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.185], "Focus": [17741.3], "XYStage": [ 37698.4, 32041.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos4", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.16], "Focus": [17741.5], "XYStage": [ 32375.4, 31758.9 ] }, "GridColumnIndex": 0 }, { "Label": "Pos5", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.17000000000002], "Focus": [17741.3], "XYStage": [ 31770.7, 31758.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos6", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.4], "XYStage": [ 26536.800000000003, 31068.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos7", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.205], "Focus": [17741.4], "XYStage": [ 25420.2, 31068.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos8", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.85], "Focus": [17741.3], "XYStage": [ 21059, 31508 ] }, "GridColumnIndex": 0 }, { "Label": "Pos9", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.82], "Focus": [17741.3], "XYStage": [ 19731.9, 31506.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos10", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.79], "Focus": [17741.4], "XYStage": [ 14528.5, 32007.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos11", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.83], "Focus": [17741.2], "XYStage": [ 13830.300000000001, 32009.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos12", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.79], "Focus": [17741.3], "XYStage": [ 8775.300000000001, 32017.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos13", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.815], "Focus": [17741.3], "XYStage": [ 8109.200000000001, 32008.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos14", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.805], "Focus": [17741.3], "XYStage": [ 3483.3, 31908.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos15", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.82], "Focus": [17741.2], "XYStage": [ 2686.8, 31903.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos16", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [211.48000000000002], "Focus": [17741.1], "XYStage": [ -1978.9, 31973.600000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos17", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [211.51500000000001], "Focus": [17741.1], "XYStage": [ -2957.8, 31982.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos18", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.195], "Focus": [17741.5], "XYStage": [ 47747.4, -576.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos19", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.23000000000002], "Focus": [17741.6], "XYStage": [ 47053.9, -576.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos20", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.205], "Focus": [17741.4], "XYStage": [ 41871.200000000004, -479.8 ] }, "GridColumnIndex": 0 }, { "Label": "Pos21", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.4], "XYStage": [ 40882.600000000006, -392 ] }, "GridColumnIndex": 0 }, { "Label": "Pos22", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.24], "Focus": [17741.4], "XYStage": [ 35905.6, -135.3 ] }, "GridColumnIndex": 0 }, { "Label": "Pos23", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.195], "Focus": [17741.5], "XYStage": [ 34990.9, -132.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos24", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.46], "Focus": [17741.4], "XYStage": [ 29678.100000000002, -958.1 ] }, "GridColumnIndex": 0 }, { "Label": "Pos25", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.44], "Focus": [17741.5], "XYStage": [ 28983.600000000002, -939.4000000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos26", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.475], "Focus": [17741.5], "XYStage": [ 23019.7, -122 ] }, "GridColumnIndex": 0 }, { "Label": "Pos27", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.44], "Focus": [17741.7], "XYStage": [ 22428, -116.80000000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos28", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.46], "Focus": [17741.7], "XYStage": [ 16364.900000000001, -181.10000000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos29", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.48000000000002], "Focus": [17741.5], "XYStage": [ 15712.1, -180 ] }, "GridColumnIndex": 0 }, { "Label": "Pos30", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.45000000000002], "Focus": [17741.7], "XYStage": [ 10346.1, 77.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos31", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.49], "Focus": [17741.4], "XYStage": [ 9755.5, 85.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos32", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.475], "Focus": [17741.5], "XYStage": [ 4775.5, 293.90000000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos33", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.445], "Focus": [17741.4], "XYStage": [ 3954.9, 293.90000000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos34", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.455], "Focus": [17741.4], "XYStage": [ -1324.9, 545.6 ] }, "GridColumnIndex": 0 }, { "Label": "Pos35", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.445], "Focus": [17741.3], "XYStage": [ -2097, 576.6 ] }, "GridColumnIndex": 0 } ], "z-step_um": 10, "GridRow": 0, "BitDepth": 16, "Depth": 2, "Channels": 6, "Source": "Micro-Manager", "ChColors": [ -16711681, -256, -65281, -1, -1, -16776961 ], "MetadataVersion": 10, "KeepShutterOpenChannels": true, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536, 65536 ], "CustomIntervals_ms": [], "Height": 1200, "MicroManagerVersion": "1.4.23 20180219", "GridColumn": 0, "UUID": "67598d21-5db5-4842-ab60-f506f7b42c7c", "PixelType": "GRAY16", "Width": 1200, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\Yan\\20191218-YC113-S7S8-miseq", "Frames": 1, "Comment": "", "UserName": "Nikon", "PixelSize_um": 1.1, "ROI": [ 0, 0, 1200, 1200 ], "Date": "2019-12-18", "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC", "P-DAPI-high" ], "KeepShutterOpenSlices": true, "IJType": 1, "ComputerName": "ZadorConfocal2", "Positions": 36, "PositionIndex": 29, "CameraTimeout": 20000, "TimeFirst": false }, "640 AO-Name": "AnalogIO", "Vincent-D3-Command": "Close" }, "FrameKey-0-3-6": { "COM4-StopBits": "1", "528 AO-MaxVolts": "5.0000", "XLIGHT Excitation Wheel-State": "1", "470 Shutter-Name": "AnalogIO", "Core-Shutter": "640", "555 AO-Demo": "No", "XLIGHT Dichroic-State": "2", "XYStage-StepSize-Y": "0.1000", "ZPositionUm": 228.48000000000002, "XYStage-StepSize-X": "0.1000", "PRIME-FirmwareVersion": "20.28", "PRIME-ClearCycles": "2", "520-Name": "DA Shutter", "405 Shutter-Name": "AnalogIO", "PIEZO-StepSize": "0.0050", "PRIME-ClearMode": "Pre-Exposure", "470 AO-MinVolts": "0.0000", "405 AO-SupportsTriggering": "1", "CameraChannelIndex": 0, "XYStage-StartSpeed": "500.0000", "COM9-StopBits": "2", "405 AO-Description": "NI DAC", "COM4-DelayBetweenCharsMs": "0.0000", "445 AO-Block voltage": "false", "520 AO-TriggerInputLine": "/Dev1/PFI0", "555 AO-Description": "NI DAC", "520 AO-TriggerSampleRate": "100000", "COM4-Verbose": "1", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM4-Name": "COM4", "405 Shutter-Volts": "0.0000", "Vincent-D3-Port": "COM1", "PRIME-PP 4 MIN ADU AFFECTED": "200", "470 Shutter-MinVolts": "0.0000", "PRIME-PP 3 ENABLED": "Yes", "470 AO-Description": "NI DAC", "PRIME-Description": "PVCAM API device adapter", "520 Shutter-MaxVolts": "5.0000", "520 Shutter-Volts": "0.0000", "528 AO-Sequenceable": "1", "PRIME-Binning": "1x1", "555 Shutter-MaxVolts": "5.0000", "528 Shutter-TriggerSequenceLength": "1000", "405 Shutter-Block voltage": "false", "XLIGHT Spin motor-State": "1", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "Vincent-D3-Time to open (ms)": "35.0000", "Vincent-D3-Time to close (ms)": "35.0000", "445 AO-TriggerSampleRate": "100000", "640 Shutter-IOChannel": "Dev1/ao14", "555 AO-Sequenceable": "1", "520 AO-Name": "AnalogIO", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "470 AO-Block voltage": "false", "COM1-Verbose": "1", "COM9-AnswerTimeout": "10000.0000", "528 Shutter-Name": "AnalogIO", "PRIME-Trigger Timeout (secs)": "10", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "NextFrame": 0, "470 AO-TriggerSequenceLength": "1000", "PRIME-TransposeXY": "0", "445 AO-TriggerSequenceLength": "1000", "COM1-Name": "COM1", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "COM9-DataBits": "8", "COM1-AnswerTimeout": "500.0000", "Camera": "", "TE2000-Version": "V2.50-V2.50-V2.10", "528 Shutter-Volts": "0.0000", "XLIGHT Hub-Name": "XLIGHT Hub", "PIEZO-Description": "Ludl stage driver adapter", "PositionName": "Pos29", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSampleRate": "100000", "PRIME-FanSpeedSetpoint": "High", "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "405 AO-TriggerSampleRate": "100000", "470 Shutter-Block voltage": "false", "470-State": "0", "COM3-Parity": "None", "405 AO-Volts": "0.0000", "PRIME-TransposeMirrorY": "0", "PRIME-ChipName": "GS144BSI", "470 AO-Volts": "0.0000", "PRIME-TransposeMirrorX": "0", "445 Shutter-SupportsTriggering": "0", "470 Shutter-SupportsTriggering": "0", "XLIGHT Hub-Description": "XLIGHT hub", "PRIME-PreampOffLimit": "10000", "470 AO-Sequenceable": "1", "528 Shutter-MaxVolts": "5.0000", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "Core-XYStage": "XYStage", "470 AO-TriggerSampleRate": "100000", "PRIME-Timing-PreTriggerDelayNs": "24672000.0000", "528-DA Device": "528 Shutter", "Focus-Description": "Nikon TE2000 focus adapter", "FilterBlock-State": "5", "520 AO-Demo": "No", "COM3-DelayBetweenCharsMs": "0.0000", "470 Shutter-Volts": "0.0000", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "PRIME-PP 2 THRESHOLD": "125", "PRIME-PP 4 THRESHOLD": "75", "XYStage-Acceleration": "4.0000", "470 Shutter-Demo": "No", "PRIME-Name": "PMPCIECam00", "640-DA Device": "640 Shutter", "405 AO-Block voltage": "false", "ElapsedTime-ms": 1214205, "PRIME-Offset": "100", "445 AO-IOChannel": "Dev1/ao1", "640 AO-MaxVolts": "5.0000", "PositionIndex": 29, "PRIME-PP 1 ENABLED": "Yes", "COM1-BaudRate": "9600", "640-State": "1", "COM3-Verbose": "1", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "LudlController-TransmissionDelay": "0", "PRIME-FullWellCapacity": "62000", "PRIME-SMARTStreamingEnabled": "No", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "640 Shutter-Description": "NI DAC", "528-Description": "DA device that is used as a shutter", "COM4-Description": "Serial port driver (boost:asio)", "405 Shutter-Description": "NI DAC", "TE2000-Name": "TE2000", "528 Shutter-Description": "NI DAC", "COM9-Description": "Serial port driver (boost:asio)", "LudlController-Name": "LudlController", "445 Shutter-TriggerSequenceLength": "1000", "PRIME-SerialNumber": "A18B203008", "445 AO-Volts": "0.0000", "PRIME-X-dimension": "1200", "PRIME-ShutterCloseDelay": "0", "XLIGHT Disk slider-State": "2", "Position": "Default", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 AO-IOChannel": "Dev1/ao5", "520 AO-IOChannel": "Dev1/ao4", "528 AO-TriggerSequenceLength": "1000", "405 Shutter-MaxVolts": "5.0000", "470-DA Device": "470 Shutter", "PRIME-FTCapable": "No", "Core-ChannelGroup": "Channels", "555 AO-SupportsTriggering": "0", "PRIME-Y-dimension": "1200", "Frame": 0, "555 Shutter-Sequenceable": "1", "PRIME-Timing-ExposureTimeNs": "499998000.0000", "Core-ImageProcessor": "", "528-State": "0", "520 AO-Block voltage": "false", "XLIGHT Excitation Wheel-Label": "QUAD", "640 Shutter-Demo": "No", "COM3-StopBits": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "520 Shutter-IOChannel": "Dev1/ao11", "Width": 1200, "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-SupportsTriggering": "0", "640 AO-TriggerSampleRate": "100000", "520-Description": "DA device that is used as a shutter", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "405 Shutter-Sequenceable": "1", "405 Shutter-SupportsTriggering": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-TriggerSampleRate": "100000", "640 Shutter-MaxVolts": "5.0000", "555 AO-MaxVolts": "5.0000", "520 AO-TriggerSequenceLength": "1000", "COM3-BaudRate": "9600", "COM9-BaudRate": "9600", "Vincent-D3-Channel #": "Ch. #1", "PRIME-PMode": "Normal", "OpticalPath-Label": "Left(100)", "BitDepth": 16, "555 AO-TriggerSequenceLength": "1000", "445 Shutter-Description": "NI DAC", "405 AO-Sequenceable": "1", "PRIME-AcquisitionMethod": "Callbacks", "555 AO-Name": "AnalogIO", "PRIME-ShutterMode": "Pre-Exposure", "COM3-Handshaking": "Off", "445 AO-SupportsTriggering": "0", "ROI": "0-0-1200-1200", "COM9-Verbose": "1", "PRIME-Color": "OFF", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "PRIME-ShutterOpenDelay": "0", "COM1-Parity": "None", "Slice": 6, "SlicePosition": 10, "COM9-DelayBetweenCharsMs": "0.0000", "555-Name": "DA Shutter", "PIEZO-LudlSingleAxisName": "B", "XLIGHT Emission Wheel-Label": "725/40 (C)", "640 Shutter-Name": "AnalogIO", "640 AO-Sequenceable": "1", "520 Shutter-Demo": "No", "528 Shutter-Demo": "No", "470-Name": "DA Shutter", "520-DA Device": "520 Shutter", "XYStage-Description": "Ludl XY stage driver adapter", "445 Shutter-MinVolts": "0.0000", "528 AO-TriggerSampleRate": "100000", "405 AO-MinVolts": "0.0000", "405 Shutter-TriggerSequenceLength": "1000", "Height": 1200, "555 AO-Block voltage": "false", "640 Shutter-Volts": "5.0000", "PixelType": "GRAY16", "445 Shutter-Block voltage": "false", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "520 Shutter-TriggerSequenceLength": "1000", "COM9-Name": "COM9", "Core-SLM": "", "555 AO-Volts": "0.0000", "555 Shutter-Block voltage": "false", "XYStage-Speed": "1800.0000", "520 AO-MaxVolts": "5.0000", "528 Shutter-SupportsTriggering": "0", "COM1-Description": "Serial port driver (boost:asio)", "PRIME-MetadataEnabled": "No", "520 Shutter-MinVolts": "0.0000", "470 Shutter-Sequenceable": "1", "PRIME-PreampDelay": "0", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-MinVolts": "0.0000", "ChannelIndex": 3, "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Core-AutoShutter": "1", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "Exposure-ms": 520, "PRIME-PP 2 ENABLED": "Yes", "640 Shutter-TriggerSampleRate": "100000", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "470 Shutter-IOChannel": "Dev1/ao10", "405 AO-Demo": "No", "PixelSizeUm": 1.1, "470 AO-Demo": "No", "470 Shutter-MaxVolts": "5.0000", "Vincent-D3-Name": "Vincent-D3", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FileName": "img_000000000_Il-C_006.tif", "LudlController-Reset": "Operate", "PRIME-Exposure": "520.00", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "445-Name": "DA Shutter", "555 Shutter-Name": "AnalogIO", "520 Shutter-TriggerSampleRate": "100000", "445 Shutter-IOChannel": "Dev1/ao9", "PRIME-TransposeCorrection": "0", "XYStage-StepSize": "0.1000", "445-Description": "DA device that is used as a shutter", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 AO-IOChannel": "Dev1/ao7", "520 Shutter-Name": "AnalogIO", "COM3-DataBits": "8", "640 AO-Volts": "4.5000", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "555 Shutter-IOChannel": "Dev1/ao13", "WaitInterval": null, "520 Shutter-Sequenceable": "1", "YPositionUm": -180, "445-State": "0", "445-DA Device": "445 Shutter", "PRIME-CircularBufferAutoSize": "ON", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "555 AO-TriggerSampleRate": "100000", "445 Shutter-Volts": "0.0000", "XLIGHT Hub-Port": "COM3", "Core-AutoFocus": "", "COM4-Handshaking": "Off", "528 Shutter-MinVolts": "0.0000", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "445 Shutter-Name": "AnalogIO", "528 Shutter-Sequenceable": "1", "XYStage-TransposeMirrorX": "0", "XYStage-TransposeMirrorY": "0", "PRIME-ReadoutRate": "100MHz 16bit", "XYStage-Name": "Stage", "640 AO-SupportsTriggering": "0", "640-Name": "DA Shutter", "640 Shutter-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "640 AO-Block voltage": "false", "445 Shutter-Sequenceable": "1", "470 AO-MaxVolts": "5.0000", "COM4-BaudRate": "9600", "470-Description": "DA device that is used as a shutter", "520 Shutter-Description": "NI DAC", "PRIME-CCDTemperature": "-14.8500", "Time": "2019-12-18 23:11:14 -0500", "640 AO-TriggerSequenceLength": "1000", "470 AO-Name": "AnalogIO", "XLIGHT Disk slider-Label": "Disk Pos 40um", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "Binning": "1x1", "TE2000-ModelType": "1", "COM3-AnswerTimeout": "500.0000", "555 Shutter-SupportsTriggering": "0", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "TE2000-Port": "COM4", "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Description": "NI DAC", "COM4-AnswerTimeout": "500.0000", "COM1-DelayBetweenCharsMs": "0.0000", "FilterBlock-Label": "Confocal", "445 AO-Name": "AnalogIO", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-Timing-ReadoutTimeNs": "24672000.0000", "528 AO-Demo": "No", "520 AO-Sequenceable": "1", "555-Description": "DA device that is used as a shutter", "405-DA Device": "405 Shutter", "405 AO-TriggerSequenceLength": "1000", "640 AO-MinVolts": "0.0000", "640 Shutter-Block voltage": "false", "FrameIndex": 0, "PRIME-Port": "CMOS", "555 AO-MinVolts": "0.0000", "COM3-Description": "Serial port driver (boost:asio)", "528 AO-Description": "NI DAC", "555 AO-IOChannel": "Dev1/ao6", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "555 AO-TriggerInputLine": "/Dev1/PFI0", "PRIME-ActualInterval-ms": "390.5200", "LudlController-Port": "COM9", "445 Shutter-Demo": "No", "PRIME-Trigger-Expose Out-Mux": "1", "SliceIndex": 6, "PRIME-CCDTemperatureSetPoint": "-15.0000", "405 Shutter-MinVolts": "0.0000", "PRIME-ExposeOutMode": "First Row", "AxisPositions": null, "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Volts": "0.0000", "528-Name": "DA Shutter", "555-State": "0", "XLIGHT Dichroic-Label": "Di03-R635-t1", "445 AO-Sequenceable": "1", "405-Name": "DA Shutter", "405 AO-IOChannel": "Dev1/ao0", "Core-Camera": "PRIME", "Focus-Name": "Focus", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "405 Shutter-IOChannel": "Dev1/ao8", "640 AO-Demo": "No", "640 Shutter-SupportsTriggering": "0", "445 AO-Demo": "No", "555-DA Device": "555 Shutter", "470 Shutter-Description": "NI DAC", "528 AO-Volts": "0.0000", "COM1-DataBits": "8", "Vincent-D3-Address": "x", "520 AO-SupportsTriggering": "0", "405-Description": "DA device that is used as a shutter", "640 Shutter-MinVolts": "0.0000", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "405 AO-Name": "AnalogIO", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "640 AO-TriggerInputLine": "/Dev1/PFI0", "405 AO-TriggerInputLine": "/Dev1/PFI0", "640-Description": "DA device that is used as a shutter", "445 Shutter-TriggerSampleRate": "100000", "470 Shutter-TriggerSequenceLength": "1000", "COM1-StopBits": "1", "520 Shutter-SupportsTriggering": "0", "405-State": "0", "Core-Initialize": "1", "XLIGHT Emission Wheel-State": "3", "520 AO-Description": "NI DAC", "528 AO-TriggerInputLine": "/Dev1/PFI0", "445 Shutter-MaxVolts": "5.0000", "COM9-Handshaking": "Off", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "XLIGHT Spin motor-Label": "On (spinning)", "PRIME-PP 5 ENABLED": "No", "UUID": "39c0aaa7-f349-4fc4-b657-0e04fd636103", "PRIME-TriggerMode": "Internal Trigger", "COM1-Handshaking": "Off", "PRIME-Timing-PostTriggerDelayNs": "49344000.0000", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "Focus-Position": "17741.5000", "528 Shutter-Block voltage": "false", "Channel": "Il-C", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "555 Shutter-Demo": "No", "405 Shutter-Demo": "No", "528 AO-Name": "AnalogIO", "520-State": "0", "Core-Galvo": "", "405 Shutter-TriggerSampleRate": "100000", "Focus-FocusStepSize_(nm)": "100", "PRIME-PixelType": "16bit", "COM4-DataBits": "8", "470 AO-IOChannel": "Dev1/ao2", "520 AO-MinVolts": "0.0000", "COM9-Parity": "None", "PRIME-CircularBufferEnabled": "ON", "PRIME-PP 6 Reset": "No", "640 AO-Description": "NI DAC", "Source": "PRIME", "445 AO-MinVolts": "0.0000", "PIEZO-Name": "Stage", "445 AO-Description": "NI DAC", "PRIME-CircularBufferFrameCount": "46", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "520 AO-Volts": "0.0000", "528 Shutter-TriggerSampleRate": "100000", "XPositionUm": 15712.1, "OpticalPath-State": "4", "PRIME-PP 3 THRESHOLD": "80", "PRIME-PVCAM Version": "3.7.1", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Gain": "1-HDR", "640 Shutter-TriggerSequenceLength": "1000", "528 AO-SupportsTriggering": "0", "445 AO-TriggerInputLine": "/Dev1/PFI0", "OpticalPath-Name": "OpticalPath", "PRIME-PP 4 ENABLED": "Yes", "PRIME-PVCAM Adapter Version": "1.3.29", "COM3-Name": "COM3", "405 AO-MaxVolts": "5.0000", "520 Shutter-Block voltage": "false", "PRIME-Timing-ClearingTimeNs": "49344000.0000", "Core-Focus": "PIEZO", "528 AO-Block voltage": "false", "TE2000-Description": "Nikon TE2000 hub", "528 AO-MinVolts": "0.0000", "COM4-Parity": "None", "Summary": { "Slices": 11, "ChContrastMin": [ 0, 0, 0, 0, 0, 0 ], "PixelAspect": 1, "SlicesFirst": true, "Interval_ms": 0, "Time": "2019-12-18 23:10:50 -0500", "Prefix": "seq_5", "InitialPositionList": [ { "Label": "Pos0", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.21], "Focus": [17741.5], "XYStage": [ 44542.200000000004, 31636.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos1", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.22], "Focus": [17741.4], "XYStage": [ 43801.100000000006, 31637.9 ] }, "GridColumnIndex": 0 }, { "Label": "Pos2", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.5], "XYStage": [ 38561.3, 32040.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos3", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.185], "Focus": [17741.3], "XYStage": [ 37698.4, 32041.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos4", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.16], "Focus": [17741.5], "XYStage": [ 32375.4, 31758.9 ] }, "GridColumnIndex": 0 }, { "Label": "Pos5", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.17000000000002], "Focus": [17741.3], "XYStage": [ 31770.7, 31758.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos6", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.4], "XYStage": [ 26536.800000000003, 31068.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos7", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.205], "Focus": [17741.4], "XYStage": [ 25420.2, 31068.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos8", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.85], "Focus": [17741.3], "XYStage": [ 21059, 31508 ] }, "GridColumnIndex": 0 }, { "Label": "Pos9", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.82], "Focus": [17741.3], "XYStage": [ 19731.9, 31506.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos10", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.79], "Focus": [17741.4], "XYStage": [ 14528.5, 32007.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos11", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.83], "Focus": [17741.2], "XYStage": [ 13830.300000000001, 32009.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos12", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.79], "Focus": [17741.3], "XYStage": [ 8775.300000000001, 32017.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos13", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.815], "Focus": [17741.3], "XYStage": [ 8109.200000000001, 32008.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos14", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.805], "Focus": [17741.3], "XYStage": [ 3483.3, 31908.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos15", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.82], "Focus": [17741.2], "XYStage": [ 2686.8, 31903.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos16", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [211.48000000000002], "Focus": [17741.1], "XYStage": [ -1978.9, 31973.600000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos17", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [211.51500000000001], "Focus": [17741.1], "XYStage": [ -2957.8, 31982.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos18", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.195], "Focus": [17741.5], "XYStage": [ 47747.4, -576.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos19", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.23000000000002], "Focus": [17741.6], "XYStage": [ 47053.9, -576.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos20", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.205], "Focus": [17741.4], "XYStage": [ 41871.200000000004, -479.8 ] }, "GridColumnIndex": 0 }, { "Label": "Pos21", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.4], "XYStage": [ 40882.600000000006, -392 ] }, "GridColumnIndex": 0 }, { "Label": "Pos22", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.24], "Focus": [17741.4], "XYStage": [ 35905.6, -135.3 ] }, "GridColumnIndex": 0 }, { "Label": "Pos23", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.195], "Focus": [17741.5], "XYStage": [ 34990.9, -132.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos24", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.46], "Focus": [17741.4], "XYStage": [ 29678.100000000002, -958.1 ] }, "GridColumnIndex": 0 }, { "Label": "Pos25", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.44], "Focus": [17741.5], "XYStage": [ 28983.600000000002, -939.4000000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos26", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.475], "Focus": [17741.5], "XYStage": [ 23019.7, -122 ] }, "GridColumnIndex": 0 }, { "Label": "Pos27", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.44], "Focus": [17741.7], "XYStage": [ 22428, -116.80000000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos28", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.46], "Focus": [17741.7], "XYStage": [ 16364.900000000001, -181.10000000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos29", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.48000000000002], "Focus": [17741.5], "XYStage": [ 15712.1, -180 ] }, "GridColumnIndex": 0 }, { "Label": "Pos30", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.45000000000002], "Focus": [17741.7], "XYStage": [ 10346.1, 77.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos31", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.49], "Focus": [17741.4], "XYStage": [ 9755.5, 85.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos32", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.475], "Focus": [17741.5], "XYStage": [ 4775.5, 293.90000000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos33", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.445], "Focus": [17741.4], "XYStage": [ 3954.9, 293.90000000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos34", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.455], "Focus": [17741.4], "XYStage": [ -1324.9, 545.6 ] }, "GridColumnIndex": 0 }, { "Label": "Pos35", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.445], "Focus": [17741.3], "XYStage": [ -2097, 576.6 ] }, "GridColumnIndex": 0 } ], "z-step_um": 10, "GridRow": 0, "BitDepth": 16, "Depth": 2, "Channels": 6, "Source": "Micro-Manager", "ChColors": [ -16711681, -256, -65281, -1, -1, -16776961 ], "MetadataVersion": 10, "KeepShutterOpenChannels": true, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536, 65536 ], "CustomIntervals_ms": [], "Height": 1200, "MicroManagerVersion": "1.4.23 20180219", "GridColumn": 0, "UUID": "67598d21-5db5-4842-ab60-f506f7b42c7c", "PixelType": "GRAY16", "Width": 1200, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\Yan\\20191218-YC113-S7S8-miseq", "Frames": 1, "Comment": "", "UserName": "Nikon", "PixelSize_um": 1.1, "ROI": [ 0, 0, 1200, 1200 ], "Date": "2019-12-18", "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC", "P-DAPI-high" ], "KeepShutterOpenSlices": true, "IJType": 1, "ComputerName": "ZadorConfocal2", "Positions": 36, "PositionIndex": 29, "CameraTimeout": 20000, "TimeFirst": false }, "640 AO-Name": "AnalogIO", "Vincent-D3-Command": "Close" }, "FrameKey-0-3-7": { "COM4-StopBits": "1", "528 AO-MaxVolts": "5.0000", "XLIGHT Excitation Wheel-State": "1", "470 Shutter-Name": "AnalogIO", "Core-Shutter": "640", "555 AO-Demo": "No", "XLIGHT Dichroic-State": "2", "XYStage-StepSize-Y": "0.1000", "ZPositionUm": 238.48000000000002, "XYStage-StepSize-X": "0.1000", "PRIME-FirmwareVersion": "20.28", "PRIME-ClearCycles": "2", "520-Name": "DA Shutter", "405 Shutter-Name": "AnalogIO", "PIEZO-StepSize": "0.0050", "PRIME-ClearMode": "Pre-Exposure", "470 AO-MinVolts": "0.0000", "405 AO-SupportsTriggering": "1", "CameraChannelIndex": 0, "XYStage-StartSpeed": "500.0000", "COM9-StopBits": "2", "405 AO-Description": "NI DAC", "COM4-DelayBetweenCharsMs": "0.0000", "445 AO-Block voltage": "false", "520 AO-TriggerInputLine": "/Dev1/PFI0", "555 AO-Description": "NI DAC", "520 AO-TriggerSampleRate": "100000", "COM4-Verbose": "1", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM4-Name": "COM4", "405 Shutter-Volts": "0.0000", "Vincent-D3-Port": "COM1", "PRIME-PP 4 MIN ADU AFFECTED": "200", "470 Shutter-MinVolts": "0.0000", "PRIME-PP 3 ENABLED": "Yes", "470 AO-Description": "NI DAC", "PRIME-Description": "PVCAM API device adapter", "520 Shutter-MaxVolts": "5.0000", "520 Shutter-Volts": "0.0000", "528 AO-Sequenceable": "1", "PRIME-Binning": "1x1", "555 Shutter-MaxVolts": "5.0000", "528 Shutter-TriggerSequenceLength": "1000", "405 Shutter-Block voltage": "false", "XLIGHT Spin motor-State": "1", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "Vincent-D3-Time to open (ms)": "35.0000", "Vincent-D3-Time to close (ms)": "35.0000", "445 AO-TriggerSampleRate": "100000", "640 Shutter-IOChannel": "Dev1/ao14", "555 AO-Sequenceable": "1", "520 AO-Name": "AnalogIO", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "470 AO-Block voltage": "false", "COM1-Verbose": "1", "COM9-AnswerTimeout": "10000.0000", "528 Shutter-Name": "AnalogIO", "PRIME-Trigger Timeout (secs)": "10", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "NextFrame": 0, "470 AO-TriggerSequenceLength": "1000", "PRIME-TransposeXY": "0", "445 AO-TriggerSequenceLength": "1000", "COM1-Name": "COM1", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "COM9-DataBits": "8", "COM1-AnswerTimeout": "500.0000", "Camera": "", "TE2000-Version": "V2.50-V2.50-V2.10", "528 Shutter-Volts": "0.0000", "XLIGHT Hub-Name": "XLIGHT Hub", "PIEZO-Description": "Ludl stage driver adapter", "PositionName": "Pos29", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSampleRate": "100000", "PRIME-FanSpeedSetpoint": "High", "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "405 AO-TriggerSampleRate": "100000", "470 Shutter-Block voltage": "false", "470-State": "0", "COM3-Parity": "None", "405 AO-Volts": "0.0000", "PRIME-TransposeMirrorY": "0", "PRIME-ChipName": "GS144BSI", "470 AO-Volts": "0.0000", "PRIME-TransposeMirrorX": "0", "445 Shutter-SupportsTriggering": "0", "470 Shutter-SupportsTriggering": "0", "XLIGHT Hub-Description": "XLIGHT hub", "PRIME-PreampOffLimit": "10000", "470 AO-Sequenceable": "1", "528 Shutter-MaxVolts": "5.0000", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "Core-XYStage": "XYStage", "470 AO-TriggerSampleRate": "100000", "PRIME-Timing-PreTriggerDelayNs": "24672000.0000", "528-DA Device": "528 Shutter", "Focus-Description": "Nikon TE2000 focus adapter", "FilterBlock-State": "5", "520 AO-Demo": "No", "COM3-DelayBetweenCharsMs": "0.0000", "470 Shutter-Volts": "0.0000", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "PRIME-PP 2 THRESHOLD": "125", "PRIME-PP 4 THRESHOLD": "75", "XYStage-Acceleration": "4.0000", "470 Shutter-Demo": "No", "PRIME-Name": "PMPCIECam00", "640-DA Device": "640 Shutter", "405 AO-Block voltage": "false", "ElapsedTime-ms": 1214974, "PRIME-Offset": "100", "445 AO-IOChannel": "Dev1/ao1", "640 AO-MaxVolts": "5.0000", "PositionIndex": 29, "PRIME-PP 1 ENABLED": "Yes", "COM1-BaudRate": "9600", "640-State": "1", "COM3-Verbose": "1", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "LudlController-TransmissionDelay": "0", "PRIME-FullWellCapacity": "62000", "PRIME-SMARTStreamingEnabled": "No", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "640 Shutter-Description": "NI DAC", "528-Description": "DA device that is used as a shutter", "COM4-Description": "Serial port driver (boost:asio)", "405 Shutter-Description": "NI DAC", "TE2000-Name": "TE2000", "528 Shutter-Description": "NI DAC", "COM9-Description": "Serial port driver (boost:asio)", "LudlController-Name": "LudlController", "445 Shutter-TriggerSequenceLength": "1000", "PRIME-SerialNumber": "A18B203008", "445 AO-Volts": "0.0000", "PRIME-X-dimension": "1200", "PRIME-ShutterCloseDelay": "0", "XLIGHT Disk slider-State": "2", "Position": "Default", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 AO-IOChannel": "Dev1/ao5", "520 AO-IOChannel": "Dev1/ao4", "528 AO-TriggerSequenceLength": "1000", "405 Shutter-MaxVolts": "5.0000", "470-DA Device": "470 Shutter", "PRIME-FTCapable": "No", "Core-ChannelGroup": "Channels", "555 AO-SupportsTriggering": "0", "PRIME-Y-dimension": "1200", "Frame": 0, "555 Shutter-Sequenceable": "1", "PRIME-Timing-ExposureTimeNs": "499998000.0000", "Core-ImageProcessor": "", "528-State": "0", "520 AO-Block voltage": "false", "XLIGHT Excitation Wheel-Label": "QUAD", "640 Shutter-Demo": "No", "COM3-StopBits": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "520 Shutter-IOChannel": "Dev1/ao11", "Width": 1200, "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-SupportsTriggering": "0", "640 AO-TriggerSampleRate": "100000", "520-Description": "DA device that is used as a shutter", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "405 Shutter-Sequenceable": "1", "405 Shutter-SupportsTriggering": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-TriggerSampleRate": "100000", "640 Shutter-MaxVolts": "5.0000", "555 AO-MaxVolts": "5.0000", "520 AO-TriggerSequenceLength": "1000", "COM3-BaudRate": "9600", "COM9-BaudRate": "9600", "Vincent-D3-Channel #": "Ch. #1", "PRIME-PMode": "Normal", "OpticalPath-Label": "Left(100)", "BitDepth": 16, "555 AO-TriggerSequenceLength": "1000", "445 Shutter-Description": "NI DAC", "405 AO-Sequenceable": "1", "PRIME-AcquisitionMethod": "Callbacks", "555 AO-Name": "AnalogIO", "PRIME-ShutterMode": "Pre-Exposure", "COM3-Handshaking": "Off", "445 AO-SupportsTriggering": "0", "ROI": "0-0-1200-1200", "COM9-Verbose": "1", "PRIME-Color": "OFF", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "PRIME-ShutterOpenDelay": "0", "COM1-Parity": "None", "Slice": 7, "SlicePosition": 20, "COM9-DelayBetweenCharsMs": "0.0000", "555-Name": "DA Shutter", "PIEZO-LudlSingleAxisName": "B", "XLIGHT Emission Wheel-Label": "725/40 (C)", "640 Shutter-Name": "AnalogIO", "640 AO-Sequenceable": "1", "520 Shutter-Demo": "No", "528 Shutter-Demo": "No", "470-Name": "DA Shutter", "520-DA Device": "520 Shutter", "XYStage-Description": "Ludl XY stage driver adapter", "445 Shutter-MinVolts": "0.0000", "528 AO-TriggerSampleRate": "100000", "405 AO-MinVolts": "0.0000", "405 Shutter-TriggerSequenceLength": "1000", "Height": 1200, "555 AO-Block voltage": "false", "640 Shutter-Volts": "5.0000", "PixelType": "GRAY16", "445 Shutter-Block voltage": "false", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "520 Shutter-TriggerSequenceLength": "1000", "COM9-Name": "COM9", "Core-SLM": "", "555 AO-Volts": "0.0000", "555 Shutter-Block voltage": "false", "XYStage-Speed": "1800.0000", "520 AO-MaxVolts": "5.0000", "528 Shutter-SupportsTriggering": "0", "COM1-Description": "Serial port driver (boost:asio)", "PRIME-MetadataEnabled": "No", "520 Shutter-MinVolts": "0.0000", "470 Shutter-Sequenceable": "1", "PRIME-PreampDelay": "0", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-MinVolts": "0.0000", "ChannelIndex": 3, "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Core-AutoShutter": "1", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "Exposure-ms": 520, "PRIME-PP 2 ENABLED": "Yes", "640 Shutter-TriggerSampleRate": "100000", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "470 Shutter-IOChannel": "Dev1/ao10", "405 AO-Demo": "No", "PixelSizeUm": 1.1, "470 AO-Demo": "No", "470 Shutter-MaxVolts": "5.0000", "Vincent-D3-Name": "Vincent-D3", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FileName": "img_000000000_Il-C_007.tif", "LudlController-Reset": "Operate", "PRIME-Exposure": "520.00", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "445-Name": "DA Shutter", "555 Shutter-Name": "AnalogIO", "520 Shutter-TriggerSampleRate": "100000", "445 Shutter-IOChannel": "Dev1/ao9", "PRIME-TransposeCorrection": "0", "XYStage-StepSize": "0.1000", "445-Description": "DA device that is used as a shutter", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 AO-IOChannel": "Dev1/ao7", "520 Shutter-Name": "AnalogIO", "COM3-DataBits": "8", "640 AO-Volts": "4.5000", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "555 Shutter-IOChannel": "Dev1/ao13", "WaitInterval": null, "520 Shutter-Sequenceable": "1", "YPositionUm": -180, "445-State": "0", "445-DA Device": "445 Shutter", "PRIME-CircularBufferAutoSize": "ON", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "555 AO-TriggerSampleRate": "100000", "445 Shutter-Volts": "0.0000", "XLIGHT Hub-Port": "COM3", "Core-AutoFocus": "", "COM4-Handshaking": "Off", "528 Shutter-MinVolts": "0.0000", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "445 Shutter-Name": "AnalogIO", "528 Shutter-Sequenceable": "1", "XYStage-TransposeMirrorX": "0", "XYStage-TransposeMirrorY": "0", "PRIME-ReadoutRate": "100MHz 16bit", "XYStage-Name": "Stage", "640 AO-SupportsTriggering": "0", "640-Name": "DA Shutter", "640 Shutter-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "640 AO-Block voltage": "false", "445 Shutter-Sequenceable": "1", "470 AO-MaxVolts": "5.0000", "COM4-BaudRate": "9600", "470-Description": "DA device that is used as a shutter", "520 Shutter-Description": "NI DAC", "PRIME-CCDTemperature": "-14.8500", "Time": "2019-12-18 23:11:15 -0500", "640 AO-TriggerSequenceLength": "1000", "470 AO-Name": "AnalogIO", "XLIGHT Disk slider-Label": "Disk Pos 40um", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "Binning": "1x1", "TE2000-ModelType": "1", "COM3-AnswerTimeout": "500.0000", "555 Shutter-SupportsTriggering": "0", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "TE2000-Port": "COM4", "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Description": "NI DAC", "COM4-AnswerTimeout": "500.0000", "COM1-DelayBetweenCharsMs": "0.0000", "FilterBlock-Label": "Confocal", "445 AO-Name": "AnalogIO", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-Timing-ReadoutTimeNs": "24672000.0000", "528 AO-Demo": "No", "520 AO-Sequenceable": "1", "555-Description": "DA device that is used as a shutter", "405-DA Device": "405 Shutter", "405 AO-TriggerSequenceLength": "1000", "640 AO-MinVolts": "0.0000", "640 Shutter-Block voltage": "false", "FrameIndex": 0, "PRIME-Port": "CMOS", "555 AO-MinVolts": "0.0000", "COM3-Description": "Serial port driver (boost:asio)", "528 AO-Description": "NI DAC", "555 AO-IOChannel": "Dev1/ao6", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "555 AO-TriggerInputLine": "/Dev1/PFI0", "PRIME-ActualInterval-ms": "390.5200", "LudlController-Port": "COM9", "445 Shutter-Demo": "No", "PRIME-Trigger-Expose Out-Mux": "1", "SliceIndex": 7, "PRIME-CCDTemperatureSetPoint": "-15.0000", "405 Shutter-MinVolts": "0.0000", "PRIME-ExposeOutMode": "First Row", "AxisPositions": null, "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Volts": "0.0000", "528-Name": "DA Shutter", "555-State": "0", "XLIGHT Dichroic-Label": "Di03-R635-t1", "445 AO-Sequenceable": "1", "405-Name": "DA Shutter", "405 AO-IOChannel": "Dev1/ao0", "Core-Camera": "PRIME", "Focus-Name": "Focus", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "405 Shutter-IOChannel": "Dev1/ao8", "640 AO-Demo": "No", "640 Shutter-SupportsTriggering": "0", "445 AO-Demo": "No", "555-DA Device": "555 Shutter", "470 Shutter-Description": "NI DAC", "528 AO-Volts": "0.0000", "COM1-DataBits": "8", "Vincent-D3-Address": "x", "520 AO-SupportsTriggering": "0", "405-Description": "DA device that is used as a shutter", "640 Shutter-MinVolts": "0.0000", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "405 AO-Name": "AnalogIO", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "640 AO-TriggerInputLine": "/Dev1/PFI0", "405 AO-TriggerInputLine": "/Dev1/PFI0", "640-Description": "DA device that is used as a shutter", "445 Shutter-TriggerSampleRate": "100000", "470 Shutter-TriggerSequenceLength": "1000", "COM1-StopBits": "1", "520 Shutter-SupportsTriggering": "0", "405-State": "0", "Core-Initialize": "1", "XLIGHT Emission Wheel-State": "3", "520 AO-Description": "NI DAC", "528 AO-TriggerInputLine": "/Dev1/PFI0", "445 Shutter-MaxVolts": "5.0000", "COM9-Handshaking": "Off", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "XLIGHT Spin motor-Label": "On (spinning)", "PRIME-PP 5 ENABLED": "No", "UUID": "f2292e7f-18b8-4fcc-8d69-e3bd69de30f6", "PRIME-TriggerMode": "Internal Trigger", "COM1-Handshaking": "Off", "PRIME-Timing-PostTriggerDelayNs": "49344000.0000", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "Focus-Position": "17741.5000", "528 Shutter-Block voltage": "false", "Channel": "Il-C", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "555 Shutter-Demo": "No", "405 Shutter-Demo": "No", "528 AO-Name": "AnalogIO", "520-State": "0", "Core-Galvo": "", "405 Shutter-TriggerSampleRate": "100000", "Focus-FocusStepSize_(nm)": "100", "PRIME-PixelType": "16bit", "COM4-DataBits": "8", "470 AO-IOChannel": "Dev1/ao2", "520 AO-MinVolts": "0.0000", "COM9-Parity": "None", "PRIME-CircularBufferEnabled": "ON", "PRIME-PP 6 Reset": "No", "640 AO-Description": "NI DAC", "Source": "PRIME", "445 AO-MinVolts": "0.0000", "PIEZO-Name": "Stage", "445 AO-Description": "NI DAC", "PRIME-CircularBufferFrameCount": "46", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "520 AO-Volts": "0.0000", "528 Shutter-TriggerSampleRate": "100000", "XPositionUm": 15712.1, "OpticalPath-State": "4", "PRIME-PP 3 THRESHOLD": "80", "PRIME-PVCAM Version": "3.7.1", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Gain": "1-HDR", "640 Shutter-TriggerSequenceLength": "1000", "528 AO-SupportsTriggering": "0", "445 AO-TriggerInputLine": "/Dev1/PFI0", "OpticalPath-Name": "OpticalPath", "PRIME-PP 4 ENABLED": "Yes", "PRIME-PVCAM Adapter Version": "1.3.29", "COM3-Name": "COM3", "405 AO-MaxVolts": "5.0000", "520 Shutter-Block voltage": "false", "PRIME-Timing-ClearingTimeNs": "49344000.0000", "Core-Focus": "PIEZO", "528 AO-Block voltage": "false", "TE2000-Description": "Nikon TE2000 hub", "528 AO-MinVolts": "0.0000", "COM4-Parity": "None", "Summary": { "Slices": 11, "ChContrastMin": [ 0, 0, 0, 0, 0, 0 ], "PixelAspect": 1, "SlicesFirst": true, "Interval_ms": 0, "Time": "2019-12-18 23:10:50 -0500", "Prefix": "seq_5", "InitialPositionList": [ { "Label": "Pos0", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.21], "Focus": [17741.5], "XYStage": [ 44542.200000000004, 31636.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos1", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.22], "Focus": [17741.4], "XYStage": [ 43801.100000000006, 31637.9 ] }, "GridColumnIndex": 0 }, { "Label": "Pos2", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.5], "XYStage": [ 38561.3, 32040.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos3", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.185], "Focus": [17741.3], "XYStage": [ 37698.4, 32041.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos4", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.16], "Focus": [17741.5], "XYStage": [ 32375.4, 31758.9 ] }, "GridColumnIndex": 0 }, { "Label": "Pos5", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.17000000000002], "Focus": [17741.3], "XYStage": [ 31770.7, 31758.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos6", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.4], "XYStage": [ 26536.800000000003, 31068.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos7", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.205], "Focus": [17741.4], "XYStage": [ 25420.2, 31068.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos8", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.85], "Focus": [17741.3], "XYStage": [ 21059, 31508 ] }, "GridColumnIndex": 0 }, { "Label": "Pos9", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.82], "Focus": [17741.3], "XYStage": [ 19731.9, 31506.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos10", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.79], "Focus": [17741.4], "XYStage": [ 14528.5, 32007.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos11", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.83], "Focus": [17741.2], "XYStage": [ 13830.300000000001, 32009.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos12", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.79], "Focus": [17741.3], "XYStage": [ 8775.300000000001, 32017.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos13", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.815], "Focus": [17741.3], "XYStage": [ 8109.200000000001, 32008.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos14", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.805], "Focus": [17741.3], "XYStage": [ 3483.3, 31908.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos15", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.82], "Focus": [17741.2], "XYStage": [ 2686.8, 31903.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos16", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [211.48000000000002], "Focus": [17741.1], "XYStage": [ -1978.9, 31973.600000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos17", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [211.51500000000001], "Focus": [17741.1], "XYStage": [ -2957.8, 31982.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos18", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.195], "Focus": [17741.5], "XYStage": [ 47747.4, -576.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos19", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.23000000000002], "Focus": [17741.6], "XYStage": [ 47053.9, -576.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos20", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.205], "Focus": [17741.4], "XYStage": [ 41871.200000000004, -479.8 ] }, "GridColumnIndex": 0 }, { "Label": "Pos21", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.4], "XYStage": [ 40882.600000000006, -392 ] }, "GridColumnIndex": 0 }, { "Label": "Pos22", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.24], "Focus": [17741.4], "XYStage": [ 35905.6, -135.3 ] }, "GridColumnIndex": 0 }, { "Label": "Pos23", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.195], "Focus": [17741.5], "XYStage": [ 34990.9, -132.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos24", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.46], "Focus": [17741.4], "XYStage": [ 29678.100000000002, -958.1 ] }, "GridColumnIndex": 0 }, { "Label": "Pos25", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.44], "Focus": [17741.5], "XYStage": [ 28983.600000000002, -939.4000000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos26", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.475], "Focus": [17741.5], "XYStage": [ 23019.7, -122 ] }, "GridColumnIndex": 0 }, { "Label": "Pos27", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.44], "Focus": [17741.7], "XYStage": [ 22428, -116.80000000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos28", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.46], "Focus": [17741.7], "XYStage": [ 16364.900000000001, -181.10000000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos29", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.48000000000002], "Focus": [17741.5], "XYStage": [ 15712.1, -180 ] }, "GridColumnIndex": 0 }, { "Label": "Pos30", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.45000000000002], "Focus": [17741.7], "XYStage": [ 10346.1, 77.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos31", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.49], "Focus": [17741.4], "XYStage": [ 9755.5, 85.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos32", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.475], "Focus": [17741.5], "XYStage": [ 4775.5, 293.90000000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos33", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.445], "Focus": [17741.4], "XYStage": [ 3954.9, 293.90000000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos34", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.455], "Focus": [17741.4], "XYStage": [ -1324.9, 545.6 ] }, "GridColumnIndex": 0 }, { "Label": "Pos35", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.445], "Focus": [17741.3], "XYStage": [ -2097, 576.6 ] }, "GridColumnIndex": 0 } ], "z-step_um": 10, "GridRow": 0, "BitDepth": 16, "Depth": 2, "Channels": 6, "Source": "Micro-Manager", "ChColors": [ -16711681, -256, -65281, -1, -1, -16776961 ], "MetadataVersion": 10, "KeepShutterOpenChannels": true, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536, 65536 ], "CustomIntervals_ms": [], "Height": 1200, "MicroManagerVersion": "1.4.23 20180219", "GridColumn": 0, "UUID": "67598d21-5db5-4842-ab60-f506f7b42c7c", "PixelType": "GRAY16", "Width": 1200, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\Yan\\20191218-YC113-S7S8-miseq", "Frames": 1, "Comment": "", "UserName": "Nikon", "PixelSize_um": 1.1, "ROI": [ 0, 0, 1200, 1200 ], "Date": "2019-12-18", "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC", "P-DAPI-high" ], "KeepShutterOpenSlices": true, "IJType": 1, "ComputerName": "ZadorConfocal2", "Positions": 36, "PositionIndex": 29, "CameraTimeout": 20000, "TimeFirst": false }, "640 AO-Name": "AnalogIO", "Vincent-D3-Command": "Close" }, "FrameKey-0-3-8": { "COM4-StopBits": "1", "528 AO-MaxVolts": "5.0000", "XLIGHT Excitation Wheel-State": "1", "470 Shutter-Name": "AnalogIO", "Core-Shutter": "640", "555 AO-Demo": "No", "XLIGHT Dichroic-State": "2", "XYStage-StepSize-Y": "0.1000", "ZPositionUm": 248.48000000000002, "XYStage-StepSize-X": "0.1000", "PRIME-FirmwareVersion": "20.28", "PRIME-ClearCycles": "2", "520-Name": "DA Shutter", "405 Shutter-Name": "AnalogIO", "PIEZO-StepSize": "0.0050", "PRIME-ClearMode": "Pre-Exposure", "470 AO-MinVolts": "0.0000", "405 AO-SupportsTriggering": "1", "CameraChannelIndex": 0, "XYStage-StartSpeed": "500.0000", "COM9-StopBits": "2", "405 AO-Description": "NI DAC", "COM4-DelayBetweenCharsMs": "0.0000", "445 AO-Block voltage": "false", "520 AO-TriggerInputLine": "/Dev1/PFI0", "555 AO-Description": "NI DAC", "520 AO-TriggerSampleRate": "100000", "COM4-Verbose": "1", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM4-Name": "COM4", "405 Shutter-Volts": "0.0000", "Vincent-D3-Port": "COM1", "PRIME-PP 4 MIN ADU AFFECTED": "200", "470 Shutter-MinVolts": "0.0000", "PRIME-PP 3 ENABLED": "Yes", "470 AO-Description": "NI DAC", "PRIME-Description": "PVCAM API device adapter", "520 Shutter-MaxVolts": "5.0000", "520 Shutter-Volts": "0.0000", "528 AO-Sequenceable": "1", "PRIME-Binning": "1x1", "555 Shutter-MaxVolts": "5.0000", "528 Shutter-TriggerSequenceLength": "1000", "405 Shutter-Block voltage": "false", "XLIGHT Spin motor-State": "1", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "Vincent-D3-Time to open (ms)": "35.0000", "Vincent-D3-Time to close (ms)": "35.0000", "445 AO-TriggerSampleRate": "100000", "640 Shutter-IOChannel": "Dev1/ao14", "555 AO-Sequenceable": "1", "520 AO-Name": "AnalogIO", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "470 AO-Block voltage": "false", "COM1-Verbose": "1", "COM9-AnswerTimeout": "10000.0000", "528 Shutter-Name": "AnalogIO", "PRIME-Trigger Timeout (secs)": "10", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "NextFrame": 0, "470 AO-TriggerSequenceLength": "1000", "PRIME-TransposeXY": "0", "445 AO-TriggerSequenceLength": "1000", "COM1-Name": "COM1", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "COM9-DataBits": "8", "COM1-AnswerTimeout": "500.0000", "Camera": "", "TE2000-Version": "V2.50-V2.50-V2.10", "528 Shutter-Volts": "0.0000", "XLIGHT Hub-Name": "XLIGHT Hub", "PIEZO-Description": "Ludl stage driver adapter", "PositionName": "Pos29", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSampleRate": "100000", "PRIME-FanSpeedSetpoint": "High", "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "405 AO-TriggerSampleRate": "100000", "470 Shutter-Block voltage": "false", "470-State": "0", "COM3-Parity": "None", "405 AO-Volts": "0.0000", "PRIME-TransposeMirrorY": "0", "PRIME-ChipName": "GS144BSI", "470 AO-Volts": "0.0000", "PRIME-TransposeMirrorX": "0", "445 Shutter-SupportsTriggering": "0", "470 Shutter-SupportsTriggering": "0", "XLIGHT Hub-Description": "XLIGHT hub", "PRIME-PreampOffLimit": "10000", "470 AO-Sequenceable": "1", "528 Shutter-MaxVolts": "5.0000", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "Core-XYStage": "XYStage", "470 AO-TriggerSampleRate": "100000", "PRIME-Timing-PreTriggerDelayNs": "24672000.0000", "528-DA Device": "528 Shutter", "Focus-Description": "Nikon TE2000 focus adapter", "FilterBlock-State": "5", "520 AO-Demo": "No", "COM3-DelayBetweenCharsMs": "0.0000", "470 Shutter-Volts": "0.0000", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "PRIME-PP 2 THRESHOLD": "125", "PRIME-PP 4 THRESHOLD": "75", "XYStage-Acceleration": "4.0000", "470 Shutter-Demo": "No", "PRIME-Name": "PMPCIECam00", "640-DA Device": "640 Shutter", "405 AO-Block voltage": "false", "ElapsedTime-ms": 1215797, "PRIME-Offset": "100", "445 AO-IOChannel": "Dev1/ao1", "640 AO-MaxVolts": "5.0000", "PositionIndex": 29, "PRIME-PP 1 ENABLED": "Yes", "COM1-BaudRate": "9600", "640-State": "1", "COM3-Verbose": "1", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "LudlController-TransmissionDelay": "0", "PRIME-FullWellCapacity": "62000", "PRIME-SMARTStreamingEnabled": "No", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "640 Shutter-Description": "NI DAC", "528-Description": "DA device that is used as a shutter", "COM4-Description": "Serial port driver (boost:asio)", "405 Shutter-Description": "NI DAC", "TE2000-Name": "TE2000", "528 Shutter-Description": "NI DAC", "COM9-Description": "Serial port driver (boost:asio)", "LudlController-Name": "LudlController", "445 Shutter-TriggerSequenceLength": "1000", "PRIME-SerialNumber": "A18B203008", "445 AO-Volts": "0.0000", "PRIME-X-dimension": "1200", "PRIME-ShutterCloseDelay": "0", "XLIGHT Disk slider-State": "2", "Position": "Default", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 AO-IOChannel": "Dev1/ao5", "520 AO-IOChannel": "Dev1/ao4", "528 AO-TriggerSequenceLength": "1000", "405 Shutter-MaxVolts": "5.0000", "470-DA Device": "470 Shutter", "PRIME-FTCapable": "No", "Core-ChannelGroup": "Channels", "555 AO-SupportsTriggering": "0", "PRIME-Y-dimension": "1200", "Frame": 0, "555 Shutter-Sequenceable": "1", "PRIME-Timing-ExposureTimeNs": "499998000.0000", "Core-ImageProcessor": "", "528-State": "0", "520 AO-Block voltage": "false", "XLIGHT Excitation Wheel-Label": "QUAD", "640 Shutter-Demo": "No", "COM3-StopBits": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "520 Shutter-IOChannel": "Dev1/ao11", "Width": 1200, "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-SupportsTriggering": "0", "640 AO-TriggerSampleRate": "100000", "520-Description": "DA device that is used as a shutter", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "405 Shutter-Sequenceable": "1", "405 Shutter-SupportsTriggering": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-TriggerSampleRate": "100000", "640 Shutter-MaxVolts": "5.0000", "555 AO-MaxVolts": "5.0000", "520 AO-TriggerSequenceLength": "1000", "COM3-BaudRate": "9600", "COM9-BaudRate": "9600", "Vincent-D3-Channel #": "Ch. #1", "PRIME-PMode": "Normal", "OpticalPath-Label": "Left(100)", "BitDepth": 16, "555 AO-TriggerSequenceLength": "1000", "445 Shutter-Description": "NI DAC", "405 AO-Sequenceable": "1", "PRIME-AcquisitionMethod": "Callbacks", "555 AO-Name": "AnalogIO", "PRIME-ShutterMode": "Pre-Exposure", "COM3-Handshaking": "Off", "445 AO-SupportsTriggering": "0", "ROI": "0-0-1200-1200", "COM9-Verbose": "1", "PRIME-Color": "OFF", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "PRIME-ShutterOpenDelay": "0", "COM1-Parity": "None", "Slice": 8, "SlicePosition": 30, "COM9-DelayBetweenCharsMs": "0.0000", "555-Name": "DA Shutter", "PIEZO-LudlSingleAxisName": "B", "XLIGHT Emission Wheel-Label": "725/40 (C)", "640 Shutter-Name": "AnalogIO", "640 AO-Sequenceable": "1", "520 Shutter-Demo": "No", "528 Shutter-Demo": "No", "470-Name": "DA Shutter", "520-DA Device": "520 Shutter", "XYStage-Description": "Ludl XY stage driver adapter", "445 Shutter-MinVolts": "0.0000", "528 AO-TriggerSampleRate": "100000", "405 AO-MinVolts": "0.0000", "405 Shutter-TriggerSequenceLength": "1000", "Height": 1200, "555 AO-Block voltage": "false", "640 Shutter-Volts": "5.0000", "PixelType": "GRAY16", "445 Shutter-Block voltage": "false", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "520 Shutter-TriggerSequenceLength": "1000", "COM9-Name": "COM9", "Core-SLM": "", "555 AO-Volts": "0.0000", "555 Shutter-Block voltage": "false", "XYStage-Speed": "1800.0000", "520 AO-MaxVolts": "5.0000", "528 Shutter-SupportsTriggering": "0", "COM1-Description": "Serial port driver (boost:asio)", "PRIME-MetadataEnabled": "No", "520 Shutter-MinVolts": "0.0000", "470 Shutter-Sequenceable": "1", "PRIME-PreampDelay": "0", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-MinVolts": "0.0000", "ChannelIndex": 3, "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Core-AutoShutter": "1", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "Exposure-ms": 520, "PRIME-PP 2 ENABLED": "Yes", "640 Shutter-TriggerSampleRate": "100000", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "470 Shutter-IOChannel": "Dev1/ao10", "405 AO-Demo": "No", "PixelSizeUm": 1.1, "470 AO-Demo": "No", "470 Shutter-MaxVolts": "5.0000", "Vincent-D3-Name": "Vincent-D3", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FileName": "img_000000000_Il-C_008.tif", "LudlController-Reset": "Operate", "PRIME-Exposure": "520.00", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "445-Name": "DA Shutter", "555 Shutter-Name": "AnalogIO", "520 Shutter-TriggerSampleRate": "100000", "445 Shutter-IOChannel": "Dev1/ao9", "PRIME-TransposeCorrection": "0", "XYStage-StepSize": "0.1000", "445-Description": "DA device that is used as a shutter", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 AO-IOChannel": "Dev1/ao7", "520 Shutter-Name": "AnalogIO", "COM3-DataBits": "8", "640 AO-Volts": "4.5000", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "555 Shutter-IOChannel": "Dev1/ao13", "WaitInterval": null, "520 Shutter-Sequenceable": "1", "YPositionUm": -180, "445-State": "0", "445-DA Device": "445 Shutter", "PRIME-CircularBufferAutoSize": "ON", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "555 AO-TriggerSampleRate": "100000", "445 Shutter-Volts": "0.0000", "XLIGHT Hub-Port": "COM3", "Core-AutoFocus": "", "COM4-Handshaking": "Off", "528 Shutter-MinVolts": "0.0000", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "445 Shutter-Name": "AnalogIO", "528 Shutter-Sequenceable": "1", "XYStage-TransposeMirrorX": "0", "XYStage-TransposeMirrorY": "0", "PRIME-ReadoutRate": "100MHz 16bit", "XYStage-Name": "Stage", "640 AO-SupportsTriggering": "0", "640-Name": "DA Shutter", "640 Shutter-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "640 AO-Block voltage": "false", "445 Shutter-Sequenceable": "1", "470 AO-MaxVolts": "5.0000", "COM4-BaudRate": "9600", "470-Description": "DA device that is used as a shutter", "520 Shutter-Description": "NI DAC", "PRIME-CCDTemperature": "-14.8500", "Time": "2019-12-18 23:11:16 -0500", "640 AO-TriggerSequenceLength": "1000", "470 AO-Name": "AnalogIO", "XLIGHT Disk slider-Label": "Disk Pos 40um", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "Binning": "1x1", "TE2000-ModelType": "1", "COM3-AnswerTimeout": "500.0000", "555 Shutter-SupportsTriggering": "0", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "TE2000-Port": "COM4", "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Description": "NI DAC", "COM4-AnswerTimeout": "500.0000", "COM1-DelayBetweenCharsMs": "0.0000", "FilterBlock-Label": "Confocal", "445 AO-Name": "AnalogIO", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-Timing-ReadoutTimeNs": "24672000.0000", "528 AO-Demo": "No", "520 AO-Sequenceable": "1", "555-Description": "DA device that is used as a shutter", "405-DA Device": "405 Shutter", "405 AO-TriggerSequenceLength": "1000", "640 AO-MinVolts": "0.0000", "640 Shutter-Block voltage": "false", "FrameIndex": 0, "PRIME-Port": "CMOS", "555 AO-MinVolts": "0.0000", "COM3-Description": "Serial port driver (boost:asio)", "528 AO-Description": "NI DAC", "555 AO-IOChannel": "Dev1/ao6", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "555 AO-TriggerInputLine": "/Dev1/PFI0", "PRIME-ActualInterval-ms": "390.5200", "LudlController-Port": "COM9", "445 Shutter-Demo": "No", "PRIME-Trigger-Expose Out-Mux": "1", "SliceIndex": 8, "PRIME-CCDTemperatureSetPoint": "-15.0000", "405 Shutter-MinVolts": "0.0000", "PRIME-ExposeOutMode": "First Row", "AxisPositions": null, "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Volts": "0.0000", "528-Name": "DA Shutter", "555-State": "0", "XLIGHT Dichroic-Label": "Di03-R635-t1", "445 AO-Sequenceable": "1", "405-Name": "DA Shutter", "405 AO-IOChannel": "Dev1/ao0", "Core-Camera": "PRIME", "Focus-Name": "Focus", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "405 Shutter-IOChannel": "Dev1/ao8", "640 AO-Demo": "No", "640 Shutter-SupportsTriggering": "0", "445 AO-Demo": "No", "555-DA Device": "555 Shutter", "470 Shutter-Description": "NI DAC", "528 AO-Volts": "0.0000", "COM1-DataBits": "8", "Vincent-D3-Address": "x", "520 AO-SupportsTriggering": "0", "405-Description": "DA device that is used as a shutter", "640 Shutter-MinVolts": "0.0000", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "405 AO-Name": "AnalogIO", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "640 AO-TriggerInputLine": "/Dev1/PFI0", "405 AO-TriggerInputLine": "/Dev1/PFI0", "640-Description": "DA device that is used as a shutter", "445 Shutter-TriggerSampleRate": "100000", "470 Shutter-TriggerSequenceLength": "1000", "COM1-StopBits": "1", "520 Shutter-SupportsTriggering": "0", "405-State": "0", "Core-Initialize": "1", "XLIGHT Emission Wheel-State": "3", "520 AO-Description": "NI DAC", "528 AO-TriggerInputLine": "/Dev1/PFI0", "445 Shutter-MaxVolts": "5.0000", "COM9-Handshaking": "Off", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "XLIGHT Spin motor-Label": "On (spinning)", "PRIME-PP 5 ENABLED": "No", "UUID": "c55990c7-990b-4107-8fe0-c8b95d26a33b", "PRIME-TriggerMode": "Internal Trigger", "COM1-Handshaking": "Off", "PRIME-Timing-PostTriggerDelayNs": "49344000.0000", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "Focus-Position": "17741.5000", "528 Shutter-Block voltage": "false", "Channel": "Il-C", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "555 Shutter-Demo": "No", "405 Shutter-Demo": "No", "528 AO-Name": "AnalogIO", "520-State": "0", "Core-Galvo": "", "405 Shutter-TriggerSampleRate": "100000", "Focus-FocusStepSize_(nm)": "100", "PRIME-PixelType": "16bit", "COM4-DataBits": "8", "470 AO-IOChannel": "Dev1/ao2", "520 AO-MinVolts": "0.0000", "COM9-Parity": "None", "PRIME-CircularBufferEnabled": "ON", "PRIME-PP 6 Reset": "No", "640 AO-Description": "NI DAC", "Source": "PRIME", "445 AO-MinVolts": "0.0000", "PIEZO-Name": "Stage", "445 AO-Description": "NI DAC", "PRIME-CircularBufferFrameCount": "46", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "520 AO-Volts": "0.0000", "528 Shutter-TriggerSampleRate": "100000", "XPositionUm": 15712.1, "OpticalPath-State": "4", "PRIME-PP 3 THRESHOLD": "80", "PRIME-PVCAM Version": "3.7.1", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Gain": "1-HDR", "640 Shutter-TriggerSequenceLength": "1000", "528 AO-SupportsTriggering": "0", "445 AO-TriggerInputLine": "/Dev1/PFI0", "OpticalPath-Name": "OpticalPath", "PRIME-PP 4 ENABLED": "Yes", "PRIME-PVCAM Adapter Version": "1.3.29", "COM3-Name": "COM3", "405 AO-MaxVolts": "5.0000", "520 Shutter-Block voltage": "false", "PRIME-Timing-ClearingTimeNs": "49344000.0000", "Core-Focus": "PIEZO", "528 AO-Block voltage": "false", "TE2000-Description": "Nikon TE2000 hub", "528 AO-MinVolts": "0.0000", "COM4-Parity": "None", "Summary": { "Slices": 11, "ChContrastMin": [ 0, 0, 0, 0, 0, 0 ], "PixelAspect": 1, "SlicesFirst": true, "Interval_ms": 0, "Time": "2019-12-18 23:10:50 -0500", "Prefix": "seq_5", "InitialPositionList": [ { "Label": "Pos0", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.21], "Focus": [17741.5], "XYStage": [ 44542.200000000004, 31636.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos1", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.22], "Focus": [17741.4], "XYStage": [ 43801.100000000006, 31637.9 ] }, "GridColumnIndex": 0 }, { "Label": "Pos2", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.5], "XYStage": [ 38561.3, 32040.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos3", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.185], "Focus": [17741.3], "XYStage": [ 37698.4, 32041.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos4", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.16], "Focus": [17741.5], "XYStage": [ 32375.4, 31758.9 ] }, "GridColumnIndex": 0 }, { "Label": "Pos5", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.17000000000002], "Focus": [17741.3], "XYStage": [ 31770.7, 31758.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos6", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.4], "XYStage": [ 26536.800000000003, 31068.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos7", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.205], "Focus": [17741.4], "XYStage": [ 25420.2, 31068.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos8", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.85], "Focus": [17741.3], "XYStage": [ 21059, 31508 ] }, "GridColumnIndex": 0 }, { "Label": "Pos9", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.82], "Focus": [17741.3], "XYStage": [ 19731.9, 31506.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos10", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.79], "Focus": [17741.4], "XYStage": [ 14528.5, 32007.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos11", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.83], "Focus": [17741.2], "XYStage": [ 13830.300000000001, 32009.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos12", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.79], "Focus": [17741.3], "XYStage": [ 8775.300000000001, 32017.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos13", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.815], "Focus": [17741.3], "XYStage": [ 8109.200000000001, 32008.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos14", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.805], "Focus": [17741.3], "XYStage": [ 3483.3, 31908.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos15", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.82], "Focus": [17741.2], "XYStage": [ 2686.8, 31903.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos16", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [211.48000000000002], "Focus": [17741.1], "XYStage": [ -1978.9, 31973.600000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos17", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [211.51500000000001], "Focus": [17741.1], "XYStage": [ -2957.8, 31982.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos18", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.195], "Focus": [17741.5], "XYStage": [ 47747.4, -576.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos19", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.23000000000002], "Focus": [17741.6], "XYStage": [ 47053.9, -576.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos20", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.205], "Focus": [17741.4], "XYStage": [ 41871.200000000004, -479.8 ] }, "GridColumnIndex": 0 }, { "Label": "Pos21", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.4], "XYStage": [ 40882.600000000006, -392 ] }, "GridColumnIndex": 0 }, { "Label": "Pos22", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.24], "Focus": [17741.4], "XYStage": [ 35905.6, -135.3 ] }, "GridColumnIndex": 0 }, { "Label": "Pos23", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.195], "Focus": [17741.5], "XYStage": [ 34990.9, -132.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos24", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.46], "Focus": [17741.4], "XYStage": [ 29678.100000000002, -958.1 ] }, "GridColumnIndex": 0 }, { "Label": "Pos25", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.44], "Focus": [17741.5], "XYStage": [ 28983.600000000002, -939.4000000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos26", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.475], "Focus": [17741.5], "XYStage": [ 23019.7, -122 ] }, "GridColumnIndex": 0 }, { "Label": "Pos27", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.44], "Focus": [17741.7], "XYStage": [ 22428, -116.80000000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos28", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.46], "Focus": [17741.7], "XYStage": [ 16364.900000000001, -181.10000000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos29", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.48000000000002], "Focus": [17741.5], "XYStage": [ 15712.1, -180 ] }, "GridColumnIndex": 0 }, { "Label": "Pos30", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.45000000000002], "Focus": [17741.7], "XYStage": [ 10346.1, 77.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos31", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.49], "Focus": [17741.4], "XYStage": [ 9755.5, 85.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos32", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.475], "Focus": [17741.5], "XYStage": [ 4775.5, 293.90000000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos33", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.445], "Focus": [17741.4], "XYStage": [ 3954.9, 293.90000000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos34", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.455], "Focus": [17741.4], "XYStage": [ -1324.9, 545.6 ] }, "GridColumnIndex": 0 }, { "Label": "Pos35", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.445], "Focus": [17741.3], "XYStage": [ -2097, 576.6 ] }, "GridColumnIndex": 0 } ], "z-step_um": 10, "GridRow": 0, "BitDepth": 16, "Depth": 2, "Channels": 6, "Source": "Micro-Manager", "ChColors": [ -16711681, -256, -65281, -1, -1, -16776961 ], "MetadataVersion": 10, "KeepShutterOpenChannels": true, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536, 65536 ], "CustomIntervals_ms": [], "Height": 1200, "MicroManagerVersion": "1.4.23 20180219", "GridColumn": 0, "UUID": "67598d21-5db5-4842-ab60-f506f7b42c7c", "PixelType": "GRAY16", "Width": 1200, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\Yan\\20191218-YC113-S7S8-miseq", "Frames": 1, "Comment": "", "UserName": "Nikon", "PixelSize_um": 1.1, "ROI": [ 0, 0, 1200, 1200 ], "Date": "2019-12-18", "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC", "P-DAPI-high" ], "KeepShutterOpenSlices": true, "IJType": 1, "ComputerName": "ZadorConfocal2", "Positions": 36, "PositionIndex": 29, "CameraTimeout": 20000, "TimeFirst": false }, "640 AO-Name": "AnalogIO", "Vincent-D3-Command": "Close" }, "FrameKey-0-3-9": { "COM4-StopBits": "1", "528 AO-MaxVolts": "5.0000", "XLIGHT Excitation Wheel-State": "1", "470 Shutter-Name": "AnalogIO", "Core-Shutter": "640", "555 AO-Demo": "No", "XLIGHT Dichroic-State": "2", "XYStage-StepSize-Y": "0.1000", "ZPositionUm": 258.48, "XYStage-StepSize-X": "0.1000", "PRIME-FirmwareVersion": "20.28", "PRIME-ClearCycles": "2", "520-Name": "DA Shutter", "405 Shutter-Name": "AnalogIO", "PIEZO-StepSize": "0.0050", "PRIME-ClearMode": "Pre-Exposure", "470 AO-MinVolts": "0.0000", "405 AO-SupportsTriggering": "1", "CameraChannelIndex": 0, "XYStage-StartSpeed": "500.0000", "COM9-StopBits": "2", "405 AO-Description": "NI DAC", "COM4-DelayBetweenCharsMs": "0.0000", "445 AO-Block voltage": "false", "520 AO-TriggerInputLine": "/Dev1/PFI0", "555 AO-Description": "NI DAC", "520 AO-TriggerSampleRate": "100000", "COM4-Verbose": "1", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM4-Name": "COM4", "405 Shutter-Volts": "0.0000", "Vincent-D3-Port": "COM1", "PRIME-PP 4 MIN ADU AFFECTED": "200", "470 Shutter-MinVolts": "0.0000", "PRIME-PP 3 ENABLED": "Yes", "470 AO-Description": "NI DAC", "PRIME-Description": "PVCAM API device adapter", "520 Shutter-MaxVolts": "5.0000", "520 Shutter-Volts": "0.0000", "528 AO-Sequenceable": "1", "PRIME-Binning": "1x1", "555 Shutter-MaxVolts": "5.0000", "528 Shutter-TriggerSequenceLength": "1000", "405 Shutter-Block voltage": "false", "XLIGHT Spin motor-State": "1", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "Vincent-D3-Time to open (ms)": "35.0000", "Vincent-D3-Time to close (ms)": "35.0000", "445 AO-TriggerSampleRate": "100000", "640 Shutter-IOChannel": "Dev1/ao14", "555 AO-Sequenceable": "1", "520 AO-Name": "AnalogIO", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "470 AO-Block voltage": "false", "COM1-Verbose": "1", "COM9-AnswerTimeout": "10000.0000", "528 Shutter-Name": "AnalogIO", "PRIME-Trigger Timeout (secs)": "10", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "NextFrame": 0, "470 AO-TriggerSequenceLength": "1000", "PRIME-TransposeXY": "0", "445 AO-TriggerSequenceLength": "1000", "COM1-Name": "COM1", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "COM9-DataBits": "8", "COM1-AnswerTimeout": "500.0000", "Camera": "", "TE2000-Version": "V2.50-V2.50-V2.10", "528 Shutter-Volts": "0.0000", "XLIGHT Hub-Name": "XLIGHT Hub", "PIEZO-Description": "Ludl stage driver adapter", "PositionName": "Pos29", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSampleRate": "100000", "PRIME-FanSpeedSetpoint": "High", "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "405 AO-TriggerSampleRate": "100000", "470 Shutter-Block voltage": "false", "470-State": "0", "COM3-Parity": "None", "405 AO-Volts": "0.0000", "PRIME-TransposeMirrorY": "0", "PRIME-ChipName": "GS144BSI", "470 AO-Volts": "0.0000", "PRIME-TransposeMirrorX": "0", "445 Shutter-SupportsTriggering": "0", "470 Shutter-SupportsTriggering": "0", "XLIGHT Hub-Description": "XLIGHT hub", "PRIME-PreampOffLimit": "10000", "470 AO-Sequenceable": "1", "528 Shutter-MaxVolts": "5.0000", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "Core-XYStage": "XYStage", "470 AO-TriggerSampleRate": "100000", "PRIME-Timing-PreTriggerDelayNs": "24672000.0000", "528-DA Device": "528 Shutter", "Focus-Description": "Nikon TE2000 focus adapter", "FilterBlock-State": "5", "520 AO-Demo": "No", "COM3-DelayBetweenCharsMs": "0.0000", "470 Shutter-Volts": "0.0000", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "PRIME-PP 2 THRESHOLD": "125", "PRIME-PP 4 THRESHOLD": "75", "XYStage-Acceleration": "4.0000", "470 Shutter-Demo": "No", "PRIME-Name": "PMPCIECam00", "640-DA Device": "640 Shutter", "405 AO-Block voltage": "false", "ElapsedTime-ms": 1216556, "PRIME-Offset": "100", "445 AO-IOChannel": "Dev1/ao1", "640 AO-MaxVolts": "5.0000", "PositionIndex": 29, "PRIME-PP 1 ENABLED": "Yes", "COM1-BaudRate": "9600", "640-State": "1", "COM3-Verbose": "1", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "LudlController-TransmissionDelay": "0", "PRIME-FullWellCapacity": "62000", "PRIME-SMARTStreamingEnabled": "No", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "640 Shutter-Description": "NI DAC", "528-Description": "DA device that is used as a shutter", "COM4-Description": "Serial port driver (boost:asio)", "405 Shutter-Description": "NI DAC", "TE2000-Name": "TE2000", "528 Shutter-Description": "NI DAC", "COM9-Description": "Serial port driver (boost:asio)", "LudlController-Name": "LudlController", "445 Shutter-TriggerSequenceLength": "1000", "PRIME-SerialNumber": "A18B203008", "445 AO-Volts": "0.0000", "PRIME-X-dimension": "1200", "PRIME-ShutterCloseDelay": "0", "XLIGHT Disk slider-State": "2", "Position": "Default", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 AO-IOChannel": "Dev1/ao5", "520 AO-IOChannel": "Dev1/ao4", "528 AO-TriggerSequenceLength": "1000", "405 Shutter-MaxVolts": "5.0000", "470-DA Device": "470 Shutter", "PRIME-FTCapable": "No", "Core-ChannelGroup": "Channels", "555 AO-SupportsTriggering": "0", "PRIME-Y-dimension": "1200", "Frame": 0, "555 Shutter-Sequenceable": "1", "PRIME-Timing-ExposureTimeNs": "499998000.0000", "Core-ImageProcessor": "", "528-State": "0", "520 AO-Block voltage": "false", "XLIGHT Excitation Wheel-Label": "QUAD", "640 Shutter-Demo": "No", "COM3-StopBits": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "520 Shutter-IOChannel": "Dev1/ao11", "Width": 1200, "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-SupportsTriggering": "0", "640 AO-TriggerSampleRate": "100000", "520-Description": "DA device that is used as a shutter", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "405 Shutter-Sequenceable": "1", "405 Shutter-SupportsTriggering": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-TriggerSampleRate": "100000", "640 Shutter-MaxVolts": "5.0000", "555 AO-MaxVolts": "5.0000", "520 AO-TriggerSequenceLength": "1000", "COM3-BaudRate": "9600", "COM9-BaudRate": "9600", "Vincent-D3-Channel #": "Ch. #1", "PRIME-PMode": "Normal", "OpticalPath-Label": "Left(100)", "BitDepth": 16, "555 AO-TriggerSequenceLength": "1000", "445 Shutter-Description": "NI DAC", "405 AO-Sequenceable": "1", "PRIME-AcquisitionMethod": "Callbacks", "555 AO-Name": "AnalogIO", "PRIME-ShutterMode": "Pre-Exposure", "COM3-Handshaking": "Off", "445 AO-SupportsTriggering": "0", "ROI": "0-0-1200-1200", "COM9-Verbose": "1", "PRIME-Color": "OFF", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "PRIME-ShutterOpenDelay": "0", "COM1-Parity": "None", "Slice": 9, "SlicePosition": 40, "COM9-DelayBetweenCharsMs": "0.0000", "555-Name": "DA Shutter", "PIEZO-LudlSingleAxisName": "B", "XLIGHT Emission Wheel-Label": "725/40 (C)", "640 Shutter-Name": "AnalogIO", "640 AO-Sequenceable": "1", "520 Shutter-Demo": "No", "528 Shutter-Demo": "No", "470-Name": "DA Shutter", "520-DA Device": "520 Shutter", "XYStage-Description": "Ludl XY stage driver adapter", "445 Shutter-MinVolts": "0.0000", "528 AO-TriggerSampleRate": "100000", "405 AO-MinVolts": "0.0000", "405 Shutter-TriggerSequenceLength": "1000", "Height": 1200, "555 AO-Block voltage": "false", "640 Shutter-Volts": "5.0000", "PixelType": "GRAY16", "445 Shutter-Block voltage": "false", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "520 Shutter-TriggerSequenceLength": "1000", "COM9-Name": "COM9", "Core-SLM": "", "555 AO-Volts": "0.0000", "555 Shutter-Block voltage": "false", "XYStage-Speed": "1800.0000", "520 AO-MaxVolts": "5.0000", "528 Shutter-SupportsTriggering": "0", "COM1-Description": "Serial port driver (boost:asio)", "PRIME-MetadataEnabled": "No", "520 Shutter-MinVolts": "0.0000", "470 Shutter-Sequenceable": "1", "PRIME-PreampDelay": "0", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-MinVolts": "0.0000", "ChannelIndex": 3, "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Core-AutoShutter": "1", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "Exposure-ms": 520, "PRIME-PP 2 ENABLED": "Yes", "640 Shutter-TriggerSampleRate": "100000", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "470 Shutter-IOChannel": "Dev1/ao10", "405 AO-Demo": "No", "PixelSizeUm": 1.1, "470 AO-Demo": "No", "470 Shutter-MaxVolts": "5.0000", "Vincent-D3-Name": "Vincent-D3", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FileName": "img_000000000_Il-C_009.tif", "LudlController-Reset": "Operate", "PRIME-Exposure": "520.00", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "445-Name": "DA Shutter", "555 Shutter-Name": "AnalogIO", "520 Shutter-TriggerSampleRate": "100000", "445 Shutter-IOChannel": "Dev1/ao9", "PRIME-TransposeCorrection": "0", "XYStage-StepSize": "0.1000", "445-Description": "DA device that is used as a shutter", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 AO-IOChannel": "Dev1/ao7", "520 Shutter-Name": "AnalogIO", "COM3-DataBits": "8", "640 AO-Volts": "4.5000", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "555 Shutter-IOChannel": "Dev1/ao13", "WaitInterval": null, "520 Shutter-Sequenceable": "1", "YPositionUm": -180, "445-State": "0", "445-DA Device": "445 Shutter", "PRIME-CircularBufferAutoSize": "ON", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "555 AO-TriggerSampleRate": "100000", "445 Shutter-Volts": "0.0000", "XLIGHT Hub-Port": "COM3", "Core-AutoFocus": "", "COM4-Handshaking": "Off", "528 Shutter-MinVolts": "0.0000", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "445 Shutter-Name": "AnalogIO", "528 Shutter-Sequenceable": "1", "XYStage-TransposeMirrorX": "0", "XYStage-TransposeMirrorY": "0", "PRIME-ReadoutRate": "100MHz 16bit", "XYStage-Name": "Stage", "640 AO-SupportsTriggering": "0", "640-Name": "DA Shutter", "640 Shutter-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "640 AO-Block voltage": "false", "445 Shutter-Sequenceable": "1", "470 AO-MaxVolts": "5.0000", "COM4-BaudRate": "9600", "470-Description": "DA device that is used as a shutter", "520 Shutter-Description": "NI DAC", "PRIME-CCDTemperature": "-14.8500", "Time": "2019-12-18 23:11:16 -0500", "640 AO-TriggerSequenceLength": "1000", "470 AO-Name": "AnalogIO", "XLIGHT Disk slider-Label": "Disk Pos 40um", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "Binning": "1x1", "TE2000-ModelType": "1", "COM3-AnswerTimeout": "500.0000", "555 Shutter-SupportsTriggering": "0", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "TE2000-Port": "COM4", "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Description": "NI DAC", "COM4-AnswerTimeout": "500.0000", "COM1-DelayBetweenCharsMs": "0.0000", "FilterBlock-Label": "Confocal", "445 AO-Name": "AnalogIO", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-Timing-ReadoutTimeNs": "24672000.0000", "528 AO-Demo": "No", "520 AO-Sequenceable": "1", "555-Description": "DA device that is used as a shutter", "405-DA Device": "405 Shutter", "405 AO-TriggerSequenceLength": "1000", "640 AO-MinVolts": "0.0000", "640 Shutter-Block voltage": "false", "FrameIndex": 0, "PRIME-Port": "CMOS", "555 AO-MinVolts": "0.0000", "COM3-Description": "Serial port driver (boost:asio)", "528 AO-Description": "NI DAC", "555 AO-IOChannel": "Dev1/ao6", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "555 AO-TriggerInputLine": "/Dev1/PFI0", "PRIME-ActualInterval-ms": "390.5200", "LudlController-Port": "COM9", "445 Shutter-Demo": "No", "PRIME-Trigger-Expose Out-Mux": "1", "SliceIndex": 9, "PRIME-CCDTemperatureSetPoint": "-15.0000", "405 Shutter-MinVolts": "0.0000", "PRIME-ExposeOutMode": "First Row", "AxisPositions": null, "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Volts": "0.0000", "528-Name": "DA Shutter", "555-State": "0", "XLIGHT Dichroic-Label": "Di03-R635-t1", "445 AO-Sequenceable": "1", "405-Name": "DA Shutter", "405 AO-IOChannel": "Dev1/ao0", "Core-Camera": "PRIME", "Focus-Name": "Focus", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "405 Shutter-IOChannel": "Dev1/ao8", "640 AO-Demo": "No", "640 Shutter-SupportsTriggering": "0", "445 AO-Demo": "No", "555-DA Device": "555 Shutter", "470 Shutter-Description": "NI DAC", "528 AO-Volts": "0.0000", "COM1-DataBits": "8", "Vincent-D3-Address": "x", "520 AO-SupportsTriggering": "0", "405-Description": "DA device that is used as a shutter", "640 Shutter-MinVolts": "0.0000", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "405 AO-Name": "AnalogIO", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "640 AO-TriggerInputLine": "/Dev1/PFI0", "405 AO-TriggerInputLine": "/Dev1/PFI0", "640-Description": "DA device that is used as a shutter", "445 Shutter-TriggerSampleRate": "100000", "470 Shutter-TriggerSequenceLength": "1000", "COM1-StopBits": "1", "520 Shutter-SupportsTriggering": "0", "405-State": "0", "Core-Initialize": "1", "XLIGHT Emission Wheel-State": "3", "520 AO-Description": "NI DAC", "528 AO-TriggerInputLine": "/Dev1/PFI0", "445 Shutter-MaxVolts": "5.0000", "COM9-Handshaking": "Off", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "XLIGHT Spin motor-Label": "On (spinning)", "PRIME-PP 5 ENABLED": "No", "UUID": "bb08f65e-18ce-407c-9cf1-7fb4392364b5", "PRIME-TriggerMode": "Internal Trigger", "COM1-Handshaking": "Off", "PRIME-Timing-PostTriggerDelayNs": "49344000.0000", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "Focus-Position": "17741.5000", "528 Shutter-Block voltage": "false", "Channel": "Il-C", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "555 Shutter-Demo": "No", "405 Shutter-Demo": "No", "528 AO-Name": "AnalogIO", "520-State": "0", "Core-Galvo": "", "405 Shutter-TriggerSampleRate": "100000", "Focus-FocusStepSize_(nm)": "100", "PRIME-PixelType": "16bit", "COM4-DataBits": "8", "470 AO-IOChannel": "Dev1/ao2", "520 AO-MinVolts": "0.0000", "COM9-Parity": "None", "PRIME-CircularBufferEnabled": "ON", "PRIME-PP 6 Reset": "No", "640 AO-Description": "NI DAC", "Source": "PRIME", "445 AO-MinVolts": "0.0000", "PIEZO-Name": "Stage", "445 AO-Description": "NI DAC", "PRIME-CircularBufferFrameCount": "46", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "520 AO-Volts": "0.0000", "528 Shutter-TriggerSampleRate": "100000", "XPositionUm": 15712.1, "OpticalPath-State": "4", "PRIME-PP 3 THRESHOLD": "80", "PRIME-PVCAM Version": "3.7.1", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Gain": "1-HDR", "640 Shutter-TriggerSequenceLength": "1000", "528 AO-SupportsTriggering": "0", "445 AO-TriggerInputLine": "/Dev1/PFI0", "OpticalPath-Name": "OpticalPath", "PRIME-PP 4 ENABLED": "Yes", "PRIME-PVCAM Adapter Version": "1.3.29", "COM3-Name": "COM3", "405 AO-MaxVolts": "5.0000", "520 Shutter-Block voltage": "false", "PRIME-Timing-ClearingTimeNs": "49344000.0000", "Core-Focus": "PIEZO", "528 AO-Block voltage": "false", "TE2000-Description": "Nikon TE2000 hub", "528 AO-MinVolts": "0.0000", "COM4-Parity": "None", "Summary": { "Slices": 11, "ChContrastMin": [ 0, 0, 0, 0, 0, 0 ], "PixelAspect": 1, "SlicesFirst": true, "Interval_ms": 0, "Time": "2019-12-18 23:10:50 -0500", "Prefix": "seq_5", "InitialPositionList": [ { "Label": "Pos0", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.21], "Focus": [17741.5], "XYStage": [ 44542.200000000004, 31636.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos1", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.22], "Focus": [17741.4], "XYStage": [ 43801.100000000006, 31637.9 ] }, "GridColumnIndex": 0 }, { "Label": "Pos2", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.5], "XYStage": [ 38561.3, 32040.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos3", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.185], "Focus": [17741.3], "XYStage": [ 37698.4, 32041.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos4", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.16], "Focus": [17741.5], "XYStage": [ 32375.4, 31758.9 ] }, "GridColumnIndex": 0 }, { "Label": "Pos5", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.17000000000002], "Focus": [17741.3], "XYStage": [ 31770.7, 31758.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos6", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.4], "XYStage": [ 26536.800000000003, 31068.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos7", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.205], "Focus": [17741.4], "XYStage": [ 25420.2, 31068.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos8", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.85], "Focus": [17741.3], "XYStage": [ 21059, 31508 ] }, "GridColumnIndex": 0 }, { "Label": "Pos9", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.82], "Focus": [17741.3], "XYStage": [ 19731.9, 31506.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos10", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.79], "Focus": [17741.4], "XYStage": [ 14528.5, 32007.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos11", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.83], "Focus": [17741.2], "XYStage": [ 13830.300000000001, 32009.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos12", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.79], "Focus": [17741.3], "XYStage": [ 8775.300000000001, 32017.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos13", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.815], "Focus": [17741.3], "XYStage": [ 8109.200000000001, 32008.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos14", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.805], "Focus": [17741.3], "XYStage": [ 3483.3, 31908.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos15", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.82], "Focus": [17741.2], "XYStage": [ 2686.8, 31903.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos16", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [211.48000000000002], "Focus": [17741.1], "XYStage": [ -1978.9, 31973.600000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos17", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [211.51500000000001], "Focus": [17741.1], "XYStage": [ -2957.8, 31982.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos18", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.195], "Focus": [17741.5], "XYStage": [ 47747.4, -576.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos19", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.23000000000002], "Focus": [17741.6], "XYStage": [ 47053.9, -576.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos20", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.205], "Focus": [17741.4], "XYStage": [ 41871.200000000004, -479.8 ] }, "GridColumnIndex": 0 }, { "Label": "Pos21", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.4], "XYStage": [ 40882.600000000006, -392 ] }, "GridColumnIndex": 0 }, { "Label": "Pos22", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.24], "Focus": [17741.4], "XYStage": [ 35905.6, -135.3 ] }, "GridColumnIndex": 0 }, { "Label": "Pos23", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.195], "Focus": [17741.5], "XYStage": [ 34990.9, -132.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos24", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.46], "Focus": [17741.4], "XYStage": [ 29678.100000000002, -958.1 ] }, "GridColumnIndex": 0 }, { "Label": "Pos25", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.44], "Focus": [17741.5], "XYStage": [ 28983.600000000002, -939.4000000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos26", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.475], "Focus": [17741.5], "XYStage": [ 23019.7, -122 ] }, "GridColumnIndex": 0 }, { "Label": "Pos27", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.44], "Focus": [17741.7], "XYStage": [ 22428, -116.80000000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos28", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.46], "Focus": [17741.7], "XYStage": [ 16364.900000000001, -181.10000000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos29", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.48000000000002], "Focus": [17741.5], "XYStage": [ 15712.1, -180 ] }, "GridColumnIndex": 0 }, { "Label": "Pos30", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.45000000000002], "Focus": [17741.7], "XYStage": [ 10346.1, 77.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos31", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.49], "Focus": [17741.4], "XYStage": [ 9755.5, 85.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos32", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.475], "Focus": [17741.5], "XYStage": [ 4775.5, 293.90000000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos33", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.445], "Focus": [17741.4], "XYStage": [ 3954.9, 293.90000000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos34", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.455], "Focus": [17741.4], "XYStage": [ -1324.9, 545.6 ] }, "GridColumnIndex": 0 }, { "Label": "Pos35", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.445], "Focus": [17741.3], "XYStage": [ -2097, 576.6 ] }, "GridColumnIndex": 0 } ], "z-step_um": 10, "GridRow": 0, "BitDepth": 16, "Depth": 2, "Channels": 6, "Source": "Micro-Manager", "ChColors": [ -16711681, -256, -65281, -1, -1, -16776961 ], "MetadataVersion": 10, "KeepShutterOpenChannels": true, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536, 65536 ], "CustomIntervals_ms": [], "Height": 1200, "MicroManagerVersion": "1.4.23 20180219", "GridColumn": 0, "UUID": "67598d21-5db5-4842-ab60-f506f7b42c7c", "PixelType": "GRAY16", "Width": 1200, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\Yan\\20191218-YC113-S7S8-miseq", "Frames": 1, "Comment": "", "UserName": "Nikon", "PixelSize_um": 1.1, "ROI": [ 0, 0, 1200, 1200 ], "Date": "2019-12-18", "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC", "P-DAPI-high" ], "KeepShutterOpenSlices": true, "IJType": 1, "ComputerName": "ZadorConfocal2", "Positions": 36, "PositionIndex": 29, "CameraTimeout": 20000, "TimeFirst": false }, "640 AO-Name": "AnalogIO", "Vincent-D3-Command": "Close" }, "FrameKey-0-3-10": { "COM4-StopBits": "1", "528 AO-MaxVolts": "5.0000", "XLIGHT Excitation Wheel-State": "1", "470 Shutter-Name": "AnalogIO", "Core-Shutter": "640", "555 AO-Demo": "No", "XLIGHT Dichroic-State": "2", "XYStage-StepSize-Y": "0.1000", "ZPositionUm": 268.48, "XYStage-StepSize-X": "0.1000", "PRIME-FirmwareVersion": "20.28", "PRIME-ClearCycles": "2", "520-Name": "DA Shutter", "405 Shutter-Name": "AnalogIO", "PIEZO-StepSize": "0.0050", "PRIME-ClearMode": "Pre-Exposure", "470 AO-MinVolts": "0.0000", "405 AO-SupportsTriggering": "1", "CameraChannelIndex": 0, "XYStage-StartSpeed": "500.0000", "COM9-StopBits": "2", "405 AO-Description": "NI DAC", "COM4-DelayBetweenCharsMs": "0.0000", "445 AO-Block voltage": "false", "520 AO-TriggerInputLine": "/Dev1/PFI0", "555 AO-Description": "NI DAC", "520 AO-TriggerSampleRate": "100000", "COM4-Verbose": "1", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM4-Name": "COM4", "405 Shutter-Volts": "0.0000", "Vincent-D3-Port": "COM1", "PRIME-PP 4 MIN ADU AFFECTED": "200", "470 Shutter-MinVolts": "0.0000", "PRIME-PP 3 ENABLED": "Yes", "470 AO-Description": "NI DAC", "PRIME-Description": "PVCAM API device adapter", "520 Shutter-MaxVolts": "5.0000", "520 Shutter-Volts": "0.0000", "528 AO-Sequenceable": "1", "PRIME-Binning": "1x1", "555 Shutter-MaxVolts": "5.0000", "528 Shutter-TriggerSequenceLength": "1000", "405 Shutter-Block voltage": "false", "XLIGHT Spin motor-State": "1", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "Vincent-D3-Time to open (ms)": "35.0000", "Vincent-D3-Time to close (ms)": "35.0000", "445 AO-TriggerSampleRate": "100000", "640 Shutter-IOChannel": "Dev1/ao14", "555 AO-Sequenceable": "1", "520 AO-Name": "AnalogIO", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "470 AO-Block voltage": "false", "COM1-Verbose": "1", "COM9-AnswerTimeout": "10000.0000", "528 Shutter-Name": "AnalogIO", "PRIME-Trigger Timeout (secs)": "10", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "NextFrame": 0, "470 AO-TriggerSequenceLength": "1000", "PRIME-TransposeXY": "0", "445 AO-TriggerSequenceLength": "1000", "COM1-Name": "COM1", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "COM9-DataBits": "8", "COM1-AnswerTimeout": "500.0000", "Camera": "", "TE2000-Version": "V2.50-V2.50-V2.10", "528 Shutter-Volts": "0.0000", "XLIGHT Hub-Name": "XLIGHT Hub", "PIEZO-Description": "Ludl stage driver adapter", "PositionName": "Pos29", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSampleRate": "100000", "PRIME-FanSpeedSetpoint": "High", "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "405 AO-TriggerSampleRate": "100000", "470 Shutter-Block voltage": "false", "470-State": "0", "COM3-Parity": "None", "405 AO-Volts": "0.0000", "PRIME-TransposeMirrorY": "0", "PRIME-ChipName": "GS144BSI", "470 AO-Volts": "0.0000", "PRIME-TransposeMirrorX": "0", "445 Shutter-SupportsTriggering": "0", "470 Shutter-SupportsTriggering": "0", "XLIGHT Hub-Description": "XLIGHT hub", "PRIME-PreampOffLimit": "10000", "470 AO-Sequenceable": "1", "528 Shutter-MaxVolts": "5.0000", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "Core-XYStage": "XYStage", "470 AO-TriggerSampleRate": "100000", "PRIME-Timing-PreTriggerDelayNs": "24672000.0000", "528-DA Device": "528 Shutter", "Focus-Description": "Nikon TE2000 focus adapter", "FilterBlock-State": "5", "520 AO-Demo": "No", "COM3-DelayBetweenCharsMs": "0.0000", "470 Shutter-Volts": "0.0000", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "PRIME-PP 2 THRESHOLD": "125", "PRIME-PP 4 THRESHOLD": "75", "XYStage-Acceleration": "4.0000", "470 Shutter-Demo": "No", "PRIME-Name": "PMPCIECam00", "640-DA Device": "640 Shutter", "405 AO-Block voltage": "false", "ElapsedTime-ms": 1217316, "PRIME-Offset": "100", "445 AO-IOChannel": "Dev1/ao1", "640 AO-MaxVolts": "5.0000", "PositionIndex": 29, "PRIME-PP 1 ENABLED": "Yes", "COM1-BaudRate": "9600", "640-State": "0", "COM3-Verbose": "1", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "LudlController-TransmissionDelay": "0", "PRIME-FullWellCapacity": "62000", "PRIME-SMARTStreamingEnabled": "No", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "640 Shutter-Description": "NI DAC", "528-Description": "DA device that is used as a shutter", "COM4-Description": "Serial port driver (boost:asio)", "405 Shutter-Description": "NI DAC", "TE2000-Name": "TE2000", "528 Shutter-Description": "NI DAC", "COM9-Description": "Serial port driver (boost:asio)", "LudlController-Name": "LudlController", "445 Shutter-TriggerSequenceLength": "1000", "PRIME-SerialNumber": "A18B203008", "445 AO-Volts": "0.0000", "PRIME-X-dimension": "1200", "PRIME-ShutterCloseDelay": "0", "XLIGHT Disk slider-State": "2", "Position": "Default", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 AO-IOChannel": "Dev1/ao5", "520 AO-IOChannel": "Dev1/ao4", "528 AO-TriggerSequenceLength": "1000", "405 Shutter-MaxVolts": "5.0000", "470-DA Device": "470 Shutter", "PRIME-FTCapable": "No", "Core-ChannelGroup": "Channels", "555 AO-SupportsTriggering": "0", "PRIME-Y-dimension": "1200", "Frame": 0, "555 Shutter-Sequenceable": "1", "PRIME-Timing-ExposureTimeNs": "499998000.0000", "Core-ImageProcessor": "", "528-State": "0", "520 AO-Block voltage": "false", "XLIGHT Excitation Wheel-Label": "QUAD", "640 Shutter-Demo": "No", "COM3-StopBits": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "520 Shutter-IOChannel": "Dev1/ao11", "Width": 1200, "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-SupportsTriggering": "0", "640 AO-TriggerSampleRate": "100000", "520-Description": "DA device that is used as a shutter", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "405 Shutter-Sequenceable": "1", "405 Shutter-SupportsTriggering": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-TriggerSampleRate": "100000", "640 Shutter-MaxVolts": "5.0000", "555 AO-MaxVolts": "5.0000", "520 AO-TriggerSequenceLength": "1000", "COM3-BaudRate": "9600", "COM9-BaudRate": "9600", "Vincent-D3-Channel #": "Ch. #1", "PRIME-PMode": "Normal", "OpticalPath-Label": "Left(100)", "BitDepth": 16, "555 AO-TriggerSequenceLength": "1000", "445 Shutter-Description": "NI DAC", "405 AO-Sequenceable": "1", "PRIME-AcquisitionMethod": "Callbacks", "555 AO-Name": "AnalogIO", "PRIME-ShutterMode": "Pre-Exposure", "COM3-Handshaking": "Off", "445 AO-SupportsTriggering": "0", "ROI": "0-0-1200-1200", "COM9-Verbose": "1", "PRIME-Color": "OFF", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "PRIME-ShutterOpenDelay": "0", "COM1-Parity": "None", "Slice": 10, "SlicePosition": 50, "COM9-DelayBetweenCharsMs": "0.0000", "555-Name": "DA Shutter", "PIEZO-LudlSingleAxisName": "B", "XLIGHT Emission Wheel-Label": "725/40 (C)", "640 Shutter-Name": "AnalogIO", "640 AO-Sequenceable": "1", "520 Shutter-Demo": "No", "528 Shutter-Demo": "No", "470-Name": "DA Shutter", "520-DA Device": "520 Shutter", "XYStage-Description": "Ludl XY stage driver adapter", "445 Shutter-MinVolts": "0.0000", "528 AO-TriggerSampleRate": "100000", "405 AO-MinVolts": "0.0000", "405 Shutter-TriggerSequenceLength": "1000", "Height": 1200, "555 AO-Block voltage": "false", "640 Shutter-Volts": "5.0000", "PixelType": "GRAY16", "445 Shutter-Block voltage": "false", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "520 Shutter-TriggerSequenceLength": "1000", "COM9-Name": "COM9", "Core-SLM": "", "555 AO-Volts": "0.0000", "555 Shutter-Block voltage": "false", "XYStage-Speed": "1800.0000", "520 AO-MaxVolts": "5.0000", "528 Shutter-SupportsTriggering": "0", "COM1-Description": "Serial port driver (boost:asio)", "PRIME-MetadataEnabled": "No", "520 Shutter-MinVolts": "0.0000", "470 Shutter-Sequenceable": "1", "PRIME-PreampDelay": "0", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-MinVolts": "0.0000", "ChannelIndex": 3, "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Core-AutoShutter": "1", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "Exposure-ms": 520, "PRIME-PP 2 ENABLED": "Yes", "640 Shutter-TriggerSampleRate": "100000", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "470 Shutter-IOChannel": "Dev1/ao10", "405 AO-Demo": "No", "PixelSizeUm": 1.1, "470 AO-Demo": "No", "470 Shutter-MaxVolts": "5.0000", "Vincent-D3-Name": "Vincent-D3", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FileName": "img_000000000_Il-C_010.tif", "LudlController-Reset": "Operate", "PRIME-Exposure": "520.00", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "445-Name": "DA Shutter", "555 Shutter-Name": "AnalogIO", "520 Shutter-TriggerSampleRate": "100000", "445 Shutter-IOChannel": "Dev1/ao9", "PRIME-TransposeCorrection": "0", "XYStage-StepSize": "0.1000", "445-Description": "DA device that is used as a shutter", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 AO-IOChannel": "Dev1/ao7", "520 Shutter-Name": "AnalogIO", "COM3-DataBits": "8", "640 AO-Volts": "4.5000", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "555 Shutter-IOChannel": "Dev1/ao13", "WaitInterval": null, "520 Shutter-Sequenceable": "1", "YPositionUm": -180, "445-State": "0", "445-DA Device": "445 Shutter", "PRIME-CircularBufferAutoSize": "ON", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "555 AO-TriggerSampleRate": "100000", "445 Shutter-Volts": "0.0000", "XLIGHT Hub-Port": "COM3", "Core-AutoFocus": "", "COM4-Handshaking": "Off", "528 Shutter-MinVolts": "0.0000", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "445 Shutter-Name": "AnalogIO", "528 Shutter-Sequenceable": "1", "XYStage-TransposeMirrorX": "0", "XYStage-TransposeMirrorY": "0", "PRIME-ReadoutRate": "100MHz 16bit", "XYStage-Name": "Stage", "640 AO-SupportsTriggering": "0", "640-Name": "DA Shutter", "640 Shutter-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "640 AO-Block voltage": "false", "445 Shutter-Sequenceable": "1", "470 AO-MaxVolts": "5.0000", "COM4-BaudRate": "9600", "470-Description": "DA device that is used as a shutter", "520 Shutter-Description": "NI DAC", "PRIME-CCDTemperature": "-14.8500", "Time": "2019-12-18 23:11:17 -0500", "640 AO-TriggerSequenceLength": "1000", "470 AO-Name": "AnalogIO", "XLIGHT Disk slider-Label": "Disk Pos 40um", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "Binning": "1x1", "TE2000-ModelType": "1", "COM3-AnswerTimeout": "500.0000", "555 Shutter-SupportsTriggering": "0", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "TE2000-Port": "COM4", "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Description": "NI DAC", "COM4-AnswerTimeout": "500.0000", "COM1-DelayBetweenCharsMs": "0.0000", "FilterBlock-Label": "Confocal", "445 AO-Name": "AnalogIO", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-Timing-ReadoutTimeNs": "24672000.0000", "528 AO-Demo": "No", "520 AO-Sequenceable": "1", "555-Description": "DA device that is used as a shutter", "405-DA Device": "405 Shutter", "405 AO-TriggerSequenceLength": "1000", "640 AO-MinVolts": "0.0000", "640 Shutter-Block voltage": "false", "FrameIndex": 0, "PRIME-Port": "CMOS", "555 AO-MinVolts": "0.0000", "COM3-Description": "Serial port driver (boost:asio)", "528 AO-Description": "NI DAC", "555 AO-IOChannel": "Dev1/ao6", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "555 AO-TriggerInputLine": "/Dev1/PFI0", "PRIME-ActualInterval-ms": "390.5200", "LudlController-Port": "COM9", "445 Shutter-Demo": "No", "PRIME-Trigger-Expose Out-Mux": "1", "SliceIndex": 10, "PRIME-CCDTemperatureSetPoint": "-15.0000", "405 Shutter-MinVolts": "0.0000", "PRIME-ExposeOutMode": "First Row", "AxisPositions": null, "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Volts": "0.0000", "528-Name": "DA Shutter", "555-State": "0", "XLIGHT Dichroic-Label": "Di03-R635-t1", "445 AO-Sequenceable": "1", "405-Name": "DA Shutter", "405 AO-IOChannel": "Dev1/ao0", "Core-Camera": "PRIME", "Focus-Name": "Focus", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "405 Shutter-IOChannel": "Dev1/ao8", "640 AO-Demo": "No", "640 Shutter-SupportsTriggering": "0", "445 AO-Demo": "No", "555-DA Device": "555 Shutter", "470 Shutter-Description": "NI DAC", "528 AO-Volts": "0.0000", "COM1-DataBits": "8", "Vincent-D3-Address": "x", "520 AO-SupportsTriggering": "0", "405-Description": "DA device that is used as a shutter", "640 Shutter-MinVolts": "0.0000", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "405 AO-Name": "AnalogIO", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "640 AO-TriggerInputLine": "/Dev1/PFI0", "405 AO-TriggerInputLine": "/Dev1/PFI0", "640-Description": "DA device that is used as a shutter", "445 Shutter-TriggerSampleRate": "100000", "470 Shutter-TriggerSequenceLength": "1000", "COM1-StopBits": "1", "520 Shutter-SupportsTriggering": "0", "405-State": "0", "Core-Initialize": "1", "XLIGHT Emission Wheel-State": "3", "520 AO-Description": "NI DAC", "528 AO-TriggerInputLine": "/Dev1/PFI0", "445 Shutter-MaxVolts": "5.0000", "COM9-Handshaking": "Off", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "XLIGHT Spin motor-Label": "On (spinning)", "PRIME-PP 5 ENABLED": "No", "UUID": "fa5a4a54-2254-4fd8-9268-8fc58726de26", "PRIME-TriggerMode": "Internal Trigger", "COM1-Handshaking": "Off", "PRIME-Timing-PostTriggerDelayNs": "49344000.0000", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "Focus-Position": "17741.5000", "528 Shutter-Block voltage": "false", "Channel": "Il-C", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "555 Shutter-Demo": "No", "405 Shutter-Demo": "No", "528 AO-Name": "AnalogIO", "520-State": "0", "Core-Galvo": "", "405 Shutter-TriggerSampleRate": "100000", "Focus-FocusStepSize_(nm)": "100", "PRIME-PixelType": "16bit", "COM4-DataBits": "8", "470 AO-IOChannel": "Dev1/ao2", "520 AO-MinVolts": "0.0000", "COM9-Parity": "None", "PRIME-CircularBufferEnabled": "ON", "PRIME-PP 6 Reset": "No", "640 AO-Description": "NI DAC", "Source": "PRIME", "445 AO-MinVolts": "0.0000", "PIEZO-Name": "Stage", "445 AO-Description": "NI DAC", "PRIME-CircularBufferFrameCount": "46", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "520 AO-Volts": "0.0000", "528 Shutter-TriggerSampleRate": "100000", "XPositionUm": 15712.1, "OpticalPath-State": "4", "PRIME-PP 3 THRESHOLD": "80", "PRIME-PVCAM Version": "3.7.1", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Gain": "1-HDR", "640 Shutter-TriggerSequenceLength": "1000", "528 AO-SupportsTriggering": "0", "445 AO-TriggerInputLine": "/Dev1/PFI0", "OpticalPath-Name": "OpticalPath", "PRIME-PP 4 ENABLED": "Yes", "PRIME-PVCAM Adapter Version": "1.3.29", "COM3-Name": "COM3", "405 AO-MaxVolts": "5.0000", "520 Shutter-Block voltage": "false", "PRIME-Timing-ClearingTimeNs": "49344000.0000", "Core-Focus": "PIEZO", "528 AO-Block voltage": "false", "TE2000-Description": "Nikon TE2000 hub", "528 AO-MinVolts": "0.0000", "COM4-Parity": "None", "Summary": { "Slices": 11, "ChContrastMin": [ 0, 0, 0, 0, 0, 0 ], "PixelAspect": 1, "SlicesFirst": true, "Interval_ms": 0, "Time": "2019-12-18 23:10:50 -0500", "Prefix": "seq_5", "InitialPositionList": [ { "Label": "Pos0", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.21], "Focus": [17741.5], "XYStage": [ 44542.200000000004, 31636.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos1", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.22], "Focus": [17741.4], "XYStage": [ 43801.100000000006, 31637.9 ] }, "GridColumnIndex": 0 }, { "Label": "Pos2", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.5], "XYStage": [ 38561.3, 32040.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos3", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.185], "Focus": [17741.3], "XYStage": [ 37698.4, 32041.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos4", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.16], "Focus": [17741.5], "XYStage": [ 32375.4, 31758.9 ] }, "GridColumnIndex": 0 }, { "Label": "Pos5", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.17000000000002], "Focus": [17741.3], "XYStage": [ 31770.7, 31758.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos6", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.4], "XYStage": [ 26536.800000000003, 31068.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos7", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.205], "Focus": [17741.4], "XYStage": [ 25420.2, 31068.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos8", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.85], "Focus": [17741.3], "XYStage": [ 21059, 31508 ] }, "GridColumnIndex": 0 }, { "Label": "Pos9", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.82], "Focus": [17741.3], "XYStage": [ 19731.9, 31506.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos10", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.79], "Focus": [17741.4], "XYStage": [ 14528.5, 32007.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos11", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.83], "Focus": [17741.2], "XYStage": [ 13830.300000000001, 32009.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos12", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.79], "Focus": [17741.3], "XYStage": [ 8775.300000000001, 32017.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos13", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.815], "Focus": [17741.3], "XYStage": [ 8109.200000000001, 32008.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos14", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.805], "Focus": [17741.3], "XYStage": [ 3483.3, 31908.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos15", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.82], "Focus": [17741.2], "XYStage": [ 2686.8, 31903.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos16", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [211.48000000000002], "Focus": [17741.1], "XYStage": [ -1978.9, 31973.600000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos17", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [211.51500000000001], "Focus": [17741.1], "XYStage": [ -2957.8, 31982.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos18", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.195], "Focus": [17741.5], "XYStage": [ 47747.4, -576.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos19", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.23000000000002], "Focus": [17741.6], "XYStage": [ 47053.9, -576.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos20", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.205], "Focus": [17741.4], "XYStage": [ 41871.200000000004, -479.8 ] }, "GridColumnIndex": 0 }, { "Label": "Pos21", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.4], "XYStage": [ 40882.600000000006, -392 ] }, "GridColumnIndex": 0 }, { "Label": "Pos22", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.24], "Focus": [17741.4], "XYStage": [ 35905.6, -135.3 ] }, "GridColumnIndex": 0 }, { "Label": "Pos23", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.195], "Focus": [17741.5], "XYStage": [ 34990.9, -132.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos24", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.46], "Focus": [17741.4], "XYStage": [ 29678.100000000002, -958.1 ] }, "GridColumnIndex": 0 }, { "Label": "Pos25", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.44], "Focus": [17741.5], "XYStage": [ 28983.600000000002, -939.4000000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos26", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.475], "Focus": [17741.5], "XYStage": [ 23019.7, -122 ] }, "GridColumnIndex": 0 }, { "Label": "Pos27", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.44], "Focus": [17741.7], "XYStage": [ 22428, -116.80000000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos28", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.46], "Focus": [17741.7], "XYStage": [ 16364.900000000001, -181.10000000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos29", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.48000000000002], "Focus": [17741.5], "XYStage": [ 15712.1, -180 ] }, "GridColumnIndex": 0 }, { "Label": "Pos30", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.45000000000002], "Focus": [17741.7], "XYStage": [ 10346.1, 77.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos31", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.49], "Focus": [17741.4], "XYStage": [ 9755.5, 85.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos32", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.475], "Focus": [17741.5], "XYStage": [ 4775.5, 293.90000000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos33", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.445], "Focus": [17741.4], "XYStage": [ 3954.9, 293.90000000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos34", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.455], "Focus": [17741.4], "XYStage": [ -1324.9, 545.6 ] }, "GridColumnIndex": 0 }, { "Label": "Pos35", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.445], "Focus": [17741.3], "XYStage": [ -2097, 576.6 ] }, "GridColumnIndex": 0 } ], "z-step_um": 10, "GridRow": 0, "BitDepth": 16, "Depth": 2, "Channels": 6, "Source": "Micro-Manager", "ChColors": [ -16711681, -256, -65281, -1, -1, -16776961 ], "MetadataVersion": 10, "KeepShutterOpenChannels": true, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536, 65536 ], "CustomIntervals_ms": [], "Height": 1200, "MicroManagerVersion": "1.4.23 20180219", "GridColumn": 0, "UUID": "67598d21-5db5-4842-ab60-f506f7b42c7c", "PixelType": "GRAY16", "Width": 1200, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\Yan\\20191218-YC113-S7S8-miseq", "Frames": 1, "Comment": "", "UserName": "Nikon", "PixelSize_um": 1.1, "ROI": [ 0, 0, 1200, 1200 ], "Date": "2019-12-18", "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC", "P-DAPI-high" ], "KeepShutterOpenSlices": true, "IJType": 1, "ComputerName": "ZadorConfocal2", "Positions": 36, "PositionIndex": 29, "CameraTimeout": 20000, "TimeFirst": false }, "640 AO-Name": "AnalogIO", "Vincent-D3-Command": "Close" }, "FrameKey-0-4-0": { "COM4-StopBits": "1", "528 AO-MaxVolts": "5.0000", "XLIGHT Excitation Wheel-State": "1", "470 Shutter-Name": "AnalogIO", "Core-Shutter": "Vincent-D3", "555 AO-Demo": "No", "XLIGHT Dichroic-State": "2", "XYStage-StepSize-Y": "0.1000", "ZPositionUm": 168.48000000000002, "XYStage-StepSize-X": "0.1000", "PRIME-FirmwareVersion": "20.28", "PRIME-ClearCycles": "2", "520-Name": "DA Shutter", "405 Shutter-Name": "AnalogIO", "PIEZO-StepSize": "0.0050", "PRIME-ClearMode": "Pre-Exposure", "470 AO-MinVolts": "0.0000", "405 AO-SupportsTriggering": "1", "CameraChannelIndex": 0, "XYStage-StartSpeed": "500.0000", "COM9-StopBits": "2", "405 AO-Description": "NI DAC", "COM4-DelayBetweenCharsMs": "0.0000", "445 AO-Block voltage": "false", "520 AO-TriggerInputLine": "/Dev1/PFI0", "555 AO-Description": "NI DAC", "520 AO-TriggerSampleRate": "100000", "COM4-Verbose": "1", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM4-Name": "COM4", "405 Shutter-Volts": "0.0000", "Vincent-D3-Port": "COM1", "PRIME-PP 4 MIN ADU AFFECTED": "200", "470 Shutter-MinVolts": "0.0000", "PRIME-PP 3 ENABLED": "Yes", "470 AO-Description": "NI DAC", "PRIME-Description": "PVCAM API device adapter", "520 Shutter-MaxVolts": "5.0000", "520 Shutter-Volts": "0.0000", "528 AO-Sequenceable": "1", "PRIME-Binning": "1x1", "555 Shutter-MaxVolts": "5.0000", "528 Shutter-TriggerSequenceLength": "1000", "405 Shutter-Block voltage": "false", "XLIGHT Spin motor-State": "1", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "Vincent-D3-Time to open (ms)": "35.0000", "Vincent-D3-Time to close (ms)": "35.0000", "445 AO-TriggerSampleRate": "100000", "640 Shutter-IOChannel": "Dev1/ao14", "555 AO-Sequenceable": "1", "520 AO-Name": "AnalogIO", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "470 AO-Block voltage": "false", "COM1-Verbose": "1", "COM9-AnswerTimeout": "10000.0000", "528 Shutter-Name": "AnalogIO", "PRIME-Trigger Timeout (secs)": "10", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "NextFrame": 0, "470 AO-TriggerSequenceLength": "1000", "PRIME-TransposeXY": "0", "445 AO-TriggerSequenceLength": "1000", "COM1-Name": "COM1", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "COM9-DataBits": "8", "COM1-AnswerTimeout": "500.0000", "Camera": "", "TE2000-Version": "V2.50-V2.50-V2.10", "528 Shutter-Volts": "0.0000", "XLIGHT Hub-Name": "XLIGHT Hub", "PIEZO-Description": "Ludl stage driver adapter", "PositionName": "Pos29", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSampleRate": "100000", "PRIME-FanSpeedSetpoint": "High", "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "405 AO-TriggerSampleRate": "100000", "470 Shutter-Block voltage": "false", "470-State": "0", "COM3-Parity": "None", "405 AO-Volts": "0.0000", "PRIME-TransposeMirrorY": "0", "PRIME-ChipName": "GS144BSI", "470 AO-Volts": "0.0000", "PRIME-TransposeMirrorX": "0", "445 Shutter-SupportsTriggering": "0", "470 Shutter-SupportsTriggering": "0", "XLIGHT Hub-Description": "XLIGHT hub", "PRIME-PreampOffLimit": "10000", "470 AO-Sequenceable": "1", "528 Shutter-MaxVolts": "5.0000", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "Core-XYStage": "XYStage", "470 AO-TriggerSampleRate": "100000", "PRIME-Timing-PreTriggerDelayNs": "24672000.0000", "528-DA Device": "528 Shutter", "Focus-Description": "Nikon TE2000 focus adapter", "FilterBlock-State": "5", "520 AO-Demo": "No", "COM3-DelayBetweenCharsMs": "0.0000", "470 Shutter-Volts": "0.0000", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "PRIME-PP 2 THRESHOLD": "125", "PRIME-PP 4 THRESHOLD": "75", "XYStage-Acceleration": "4.0000", "470 Shutter-Demo": "No", "PRIME-Name": "PMPCIECam00", "640-DA Device": "640 Shutter", "405 AO-Block voltage": "false", "ElapsedTime-ms": 1218282, "PRIME-Offset": "100", "445 AO-IOChannel": "Dev1/ao1", "640 AO-MaxVolts": "5.0000", "PositionIndex": 29, "PRIME-PP 1 ENABLED": "Yes", "COM1-BaudRate": "9600", "640-State": "0", "COM3-Verbose": "1", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "LudlController-TransmissionDelay": "0", "PRIME-FullWellCapacity": "62000", "PRIME-SMARTStreamingEnabled": "No", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "640 Shutter-Description": "NI DAC", "528-Description": "DA device that is used as a shutter", "COM4-Description": "Serial port driver (boost:asio)", "405 Shutter-Description": "NI DAC", "TE2000-Name": "TE2000", "528 Shutter-Description": "NI DAC", "COM9-Description": "Serial port driver (boost:asio)", "LudlController-Name": "LudlController", "445 Shutter-TriggerSequenceLength": "1000", "PRIME-SerialNumber": "A18B203008", "445 AO-Volts": "0.0000", "PRIME-X-dimension": "1200", "PRIME-ShutterCloseDelay": "0", "XLIGHT Disk slider-State": "2", "Position": "Default", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 AO-IOChannel": "Dev1/ao5", "520 AO-IOChannel": "Dev1/ao4", "528 AO-TriggerSequenceLength": "1000", "405 Shutter-MaxVolts": "5.0000", "470-DA Device": "470 Shutter", "PRIME-FTCapable": "No", "Core-ChannelGroup": "Channels", "555 AO-SupportsTriggering": "0", "PRIME-Y-dimension": "1200", "Frame": 0, "555 Shutter-Sequenceable": "1", "PRIME-Timing-ExposureTimeNs": "499998000.0000", "Core-ImageProcessor": "", "528-State": "0", "520 AO-Block voltage": "false", "XLIGHT Excitation Wheel-Label": "Excitation-2", "640 Shutter-Demo": "No", "COM3-StopBits": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "520 Shutter-IOChannel": "Dev1/ao11", "Width": 1200, "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-SupportsTriggering": "0", "640 AO-TriggerSampleRate": "100000", "520-Description": "DA device that is used as a shutter", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "405 Shutter-Sequenceable": "1", "405 Shutter-SupportsTriggering": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-TriggerSampleRate": "100000", "640 Shutter-MaxVolts": "5.0000", "555 AO-MaxVolts": "5.0000", "520 AO-TriggerSequenceLength": "1000", "COM3-BaudRate": "9600", "COM9-BaudRate": "9600", "Vincent-D3-Channel #": "Ch. #1", "PRIME-PMode": "Normal", "OpticalPath-Label": "Left(100)", "BitDepth": 16, "555 AO-TriggerSequenceLength": "1000", "445 Shutter-Description": "NI DAC", "405 AO-Sequenceable": "1", "PRIME-AcquisitionMethod": "Callbacks", "555 AO-Name": "AnalogIO", "PRIME-ShutterMode": "Pre-Exposure", "COM3-Handshaking": "Off", "445 AO-SupportsTriggering": "0", "ROI": "0-0-1200-1200", "COM9-Verbose": "1", "PRIME-Color": "OFF", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "PRIME-ShutterOpenDelay": "0", "COM1-Parity": "None", "Slice": 0, "SlicePosition": -50, "COM9-DelayBetweenCharsMs": "0.0000", "555-Name": "DA Shutter", "PIEZO-LudlSingleAxisName": "B", "XLIGHT Emission Wheel-Label": "Empty", "640 Shutter-Name": "AnalogIO", "640 AO-Sequenceable": "1", "520 Shutter-Demo": "No", "528 Shutter-Demo": "No", "470-Name": "DA Shutter", "520-DA Device": "520 Shutter", "XYStage-Description": "Ludl XY stage driver adapter", "445 Shutter-MinVolts": "0.0000", "528 AO-TriggerSampleRate": "100000", "405 AO-MinVolts": "0.0000", "405 Shutter-TriggerSequenceLength": "1000", "Height": 1200, "555 AO-Block voltage": "false", "640 Shutter-Volts": "0.0000", "PixelType": "GRAY16", "445 Shutter-Block voltage": "false", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "520 Shutter-TriggerSequenceLength": "1000", "COM9-Name": "COM9", "Core-SLM": "", "555 AO-Volts": "0.0000", "555 Shutter-Block voltage": "false", "XYStage-Speed": "1800.0000", "520 AO-MaxVolts": "5.0000", "528 Shutter-SupportsTriggering": "0", "COM1-Description": "Serial port driver (boost:asio)", "PRIME-MetadataEnabled": "No", "520 Shutter-MinVolts": "0.0000", "470 Shutter-Sequenceable": "1", "PRIME-PreampDelay": "0", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-MinVolts": "0.0000", "ChannelIndex": 4, "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Core-AutoShutter": "1", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "Exposure-ms": 20, "PRIME-PP 2 ENABLED": "Yes", "640 Shutter-TriggerSampleRate": "100000", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "470 Shutter-IOChannel": "Dev1/ao10", "405 AO-Demo": "No", "PixelSizeUm": 1.1, "470 AO-Demo": "No", "470 Shutter-MaxVolts": "5.0000", "Vincent-D3-Name": "Vincent-D3", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FileName": "img_000000000_P-DIC_000.tif", "LudlController-Reset": "Operate", "PRIME-Exposure": "20.00", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "445-Name": "DA Shutter", "555 Shutter-Name": "AnalogIO", "520 Shutter-TriggerSampleRate": "100000", "445 Shutter-IOChannel": "Dev1/ao9", "PRIME-TransposeCorrection": "0", "XYStage-StepSize": "0.1000", "445-Description": "DA device that is used as a shutter", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 AO-IOChannel": "Dev1/ao7", "520 Shutter-Name": "AnalogIO", "COM3-DataBits": "8", "640 AO-Volts": "0.0000", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "555 Shutter-IOChannel": "Dev1/ao13", "WaitInterval": null, "520 Shutter-Sequenceable": "1", "YPositionUm": -180, "445-State": "0", "445-DA Device": "445 Shutter", "PRIME-CircularBufferAutoSize": "ON", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "555 AO-TriggerSampleRate": "100000", "445 Shutter-Volts": "0.0000", "XLIGHT Hub-Port": "COM3", "Core-AutoFocus": "", "COM4-Handshaking": "Off", "528 Shutter-MinVolts": "0.0000", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "445 Shutter-Name": "AnalogIO", "528 Shutter-Sequenceable": "1", "XYStage-TransposeMirrorX": "0", "XYStage-TransposeMirrorY": "0", "PRIME-ReadoutRate": "100MHz 16bit", "XYStage-Name": "Stage", "640 AO-SupportsTriggering": "0", "640-Name": "DA Shutter", "640 Shutter-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "640 AO-Block voltage": "false", "445 Shutter-Sequenceable": "1", "470 AO-MaxVolts": "5.0000", "COM4-BaudRate": "9600", "470-Description": "DA device that is used as a shutter", "520 Shutter-Description": "NI DAC", "PRIME-CCDTemperature": "-14.8500", "Time": "2019-12-18 23:11:18 -0500", "640 AO-TriggerSequenceLength": "1000", "470 AO-Name": "AnalogIO", "XLIGHT Disk slider-Label": "Disk Pos 40um", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "Binning": "1x1", "TE2000-ModelType": "1", "COM3-AnswerTimeout": "500.0000", "555 Shutter-SupportsTriggering": "0", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "TE2000-Port": "COM4", "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Description": "NI DAC", "COM4-AnswerTimeout": "500.0000", "COM1-DelayBetweenCharsMs": "0.0000", "FilterBlock-Label": "Confocal", "445 AO-Name": "AnalogIO", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-Timing-ReadoutTimeNs": "24672000.0000", "528 AO-Demo": "No", "520 AO-Sequenceable": "1", "555-Description": "DA device that is used as a shutter", "405-DA Device": "405 Shutter", "405 AO-TriggerSequenceLength": "1000", "640 AO-MinVolts": "0.0000", "640 Shutter-Block voltage": "false", "FrameIndex": 0, "PRIME-Port": "CMOS", "555 AO-MinVolts": "0.0000", "COM3-Description": "Serial port driver (boost:asio)", "528 AO-Description": "NI DAC", "555 AO-IOChannel": "Dev1/ao6", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "555 AO-TriggerInputLine": "/Dev1/PFI0", "PRIME-ActualInterval-ms": "390.5200", "LudlController-Port": "COM9", "445 Shutter-Demo": "No", "PRIME-Trigger-Expose Out-Mux": "1", "SliceIndex": 0, "PRIME-CCDTemperatureSetPoint": "-15.0000", "405 Shutter-MinVolts": "0.0000", "PRIME-ExposeOutMode": "First Row", "AxisPositions": null, "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Volts": "0.0000", "528-Name": "DA Shutter", "555-State": "0", "XLIGHT Dichroic-Label": "Di03-R635-t1", "445 AO-Sequenceable": "1", "405-Name": "DA Shutter", "405 AO-IOChannel": "Dev1/ao0", "Core-Camera": "PRIME", "Focus-Name": "Focus", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "405 Shutter-IOChannel": "Dev1/ao8", "640 AO-Demo": "No", "640 Shutter-SupportsTriggering": "0", "445 AO-Demo": "No", "555-DA Device": "555 Shutter", "470 Shutter-Description": "NI DAC", "528 AO-Volts": "0.0000", "COM1-DataBits": "8", "Vincent-D3-Address": "x", "520 AO-SupportsTriggering": "0", "405-Description": "DA device that is used as a shutter", "640 Shutter-MinVolts": "0.0000", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "405 AO-Name": "AnalogIO", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "640 AO-TriggerInputLine": "/Dev1/PFI0", "405 AO-TriggerInputLine": "/Dev1/PFI0", "640-Description": "DA device that is used as a shutter", "445 Shutter-TriggerSampleRate": "100000", "470 Shutter-TriggerSequenceLength": "1000", "COM1-StopBits": "1", "520 Shutter-SupportsTriggering": "0", "405-State": "0", "Core-Initialize": "1", "XLIGHT Emission Wheel-State": "3", "520 AO-Description": "NI DAC", "528 AO-TriggerInputLine": "/Dev1/PFI0", "445 Shutter-MaxVolts": "5.0000", "COM9-Handshaking": "Off", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "XLIGHT Spin motor-Label": "On (spinning)", "PRIME-PP 5 ENABLED": "No", "UUID": "60b75e88-ce27-4130-8bab-a515982c44d0", "PRIME-TriggerMode": "Internal Trigger", "COM1-Handshaking": "Off", "PRIME-Timing-PostTriggerDelayNs": "49344000.0000", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "Focus-Position": "17741.5000", "528 Shutter-Block voltage": "false", "Channel": "P-DIC", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "555 Shutter-Demo": "No", "405 Shutter-Demo": "No", "528 AO-Name": "AnalogIO", "520-State": "0", "Core-Galvo": "", "405 Shutter-TriggerSampleRate": "100000", "Focus-FocusStepSize_(nm)": "100", "PRIME-PixelType": "16bit", "COM4-DataBits": "8", "470 AO-IOChannel": "Dev1/ao2", "520 AO-MinVolts": "0.0000", "COM9-Parity": "None", "PRIME-CircularBufferEnabled": "ON", "PRIME-PP 6 Reset": "No", "640 AO-Description": "NI DAC", "Source": "PRIME", "445 AO-MinVolts": "0.0000", "PIEZO-Name": "Stage", "445 AO-Description": "NI DAC", "PRIME-CircularBufferFrameCount": "46", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "520 AO-Volts": "0.0000", "528 Shutter-TriggerSampleRate": "100000", "XPositionUm": 15712.1, "OpticalPath-State": "4", "PRIME-PP 3 THRESHOLD": "80", "PRIME-PVCAM Version": "3.7.1", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Gain": "1-HDR", "640 Shutter-TriggerSequenceLength": "1000", "528 AO-SupportsTriggering": "0", "445 AO-TriggerInputLine": "/Dev1/PFI0", "OpticalPath-Name": "OpticalPath", "PRIME-PP 4 ENABLED": "Yes", "PRIME-PVCAM Adapter Version": "1.3.29", "COM3-Name": "COM3", "405 AO-MaxVolts": "5.0000", "520 Shutter-Block voltage": "false", "PRIME-Timing-ClearingTimeNs": "49344000.0000", "Core-Focus": "PIEZO", "528 AO-Block voltage": "false", "TE2000-Description": "Nikon TE2000 hub", "528 AO-MinVolts": "0.0000", "COM4-Parity": "None", "Summary": { "Slices": 11, "ChContrastMin": [ 0, 0, 0, 0, 0, 0 ], "PixelAspect": 1, "SlicesFirst": true, "Interval_ms": 0, "Time": "2019-12-18 23:10:50 -0500", "Prefix": "seq_5", "InitialPositionList": [ { "Label": "Pos0", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.21], "Focus": [17741.5], "XYStage": [ 44542.200000000004, 31636.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos1", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.22], "Focus": [17741.4], "XYStage": [ 43801.100000000006, 31637.9 ] }, "GridColumnIndex": 0 }, { "Label": "Pos2", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.5], "XYStage": [ 38561.3, 32040.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos3", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.185], "Focus": [17741.3], "XYStage": [ 37698.4, 32041.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos4", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.16], "Focus": [17741.5], "XYStage": [ 32375.4, 31758.9 ] }, "GridColumnIndex": 0 }, { "Label": "Pos5", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.17000000000002], "Focus": [17741.3], "XYStage": [ 31770.7, 31758.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos6", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.4], "XYStage": [ 26536.800000000003, 31068.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos7", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.205], "Focus": [17741.4], "XYStage": [ 25420.2, 31068.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos8", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.85], "Focus": [17741.3], "XYStage": [ 21059, 31508 ] }, "GridColumnIndex": 0 }, { "Label": "Pos9", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.82], "Focus": [17741.3], "XYStage": [ 19731.9, 31506.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos10", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.79], "Focus": [17741.4], "XYStage": [ 14528.5, 32007.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos11", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.83], "Focus": [17741.2], "XYStage": [ 13830.300000000001, 32009.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos12", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.79], "Focus": [17741.3], "XYStage": [ 8775.300000000001, 32017.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos13", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.815], "Focus": [17741.3], "XYStage": [ 8109.200000000001, 32008.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos14", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.805], "Focus": [17741.3], "XYStage": [ 3483.3, 31908.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos15", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.82], "Focus": [17741.2], "XYStage": [ 2686.8, 31903.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos16", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [211.48000000000002], "Focus": [17741.1], "XYStage": [ -1978.9, 31973.600000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos17", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [211.51500000000001], "Focus": [17741.1], "XYStage": [ -2957.8, 31982.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos18", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.195], "Focus": [17741.5], "XYStage": [ 47747.4, -576.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos19", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.23000000000002], "Focus": [17741.6], "XYStage": [ 47053.9, -576.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos20", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.205], "Focus": [17741.4], "XYStage": [ 41871.200000000004, -479.8 ] }, "GridColumnIndex": 0 }, { "Label": "Pos21", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.4], "XYStage": [ 40882.600000000006, -392 ] }, "GridColumnIndex": 0 }, { "Label": "Pos22", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.24], "Focus": [17741.4], "XYStage": [ 35905.6, -135.3 ] }, "GridColumnIndex": 0 }, { "Label": "Pos23", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.195], "Focus": [17741.5], "XYStage": [ 34990.9, -132.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos24", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.46], "Focus": [17741.4], "XYStage": [ 29678.100000000002, -958.1 ] }, "GridColumnIndex": 0 }, { "Label": "Pos25", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.44], "Focus": [17741.5], "XYStage": [ 28983.600000000002, -939.4000000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos26", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.475], "Focus": [17741.5], "XYStage": [ 23019.7, -122 ] }, "GridColumnIndex": 0 }, { "Label": "Pos27", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.44], "Focus": [17741.7], "XYStage": [ 22428, -116.80000000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos28", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.46], "Focus": [17741.7], "XYStage": [ 16364.900000000001, -181.10000000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos29", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.48000000000002], "Focus": [17741.5], "XYStage": [ 15712.1, -180 ] }, "GridColumnIndex": 0 }, { "Label": "Pos30", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.45000000000002], "Focus": [17741.7], "XYStage": [ 10346.1, 77.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos31", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.49], "Focus": [17741.4], "XYStage": [ 9755.5, 85.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos32", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.475], "Focus": [17741.5], "XYStage": [ 4775.5, 293.90000000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos33", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.445], "Focus": [17741.4], "XYStage": [ 3954.9, 293.90000000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos34", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.455], "Focus": [17741.4], "XYStage": [ -1324.9, 545.6 ] }, "GridColumnIndex": 0 }, { "Label": "Pos35", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.445], "Focus": [17741.3], "XYStage": [ -2097, 576.6 ] }, "GridColumnIndex": 0 } ], "z-step_um": 10, "GridRow": 0, "BitDepth": 16, "Depth": 2, "Channels": 6, "Source": "Micro-Manager", "ChColors": [ -16711681, -256, -65281, -1, -1, -16776961 ], "MetadataVersion": 10, "KeepShutterOpenChannels": true, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536, 65536 ], "CustomIntervals_ms": [], "Height": 1200, "MicroManagerVersion": "1.4.23 20180219", "GridColumn": 0, "UUID": "67598d21-5db5-4842-ab60-f506f7b42c7c", "PixelType": "GRAY16", "Width": 1200, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\Yan\\20191218-YC113-S7S8-miseq", "Frames": 1, "Comment": "", "UserName": "Nikon", "PixelSize_um": 1.1, "ROI": [ 0, 0, 1200, 1200 ], "Date": "2019-12-18", "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC", "P-DAPI-high" ], "KeepShutterOpenSlices": true, "IJType": 1, "ComputerName": "ZadorConfocal2", "Positions": 36, "PositionIndex": 29, "CameraTimeout": 20000, "TimeFirst": false }, "640 AO-Name": "AnalogIO", "Vincent-D3-Command": "Close" }, "FrameKey-0-4-1": { "COM4-StopBits": "1", "528 AO-MaxVolts": "5.0000", "XLIGHT Excitation Wheel-State": "1", "470 Shutter-Name": "AnalogIO", "Core-Shutter": "Vincent-D3", "555 AO-Demo": "No", "XLIGHT Dichroic-State": "2", "XYStage-StepSize-Y": "0.1000", "ZPositionUm": 178.48000000000002, "XYStage-StepSize-X": "0.1000", "PRIME-FirmwareVersion": "20.28", "PRIME-ClearCycles": "2", "520-Name": "DA Shutter", "405 Shutter-Name": "AnalogIO", "PIEZO-StepSize": "0.0050", "PRIME-ClearMode": "Pre-Exposure", "470 AO-MinVolts": "0.0000", "405 AO-SupportsTriggering": "1", "CameraChannelIndex": 0, "XYStage-StartSpeed": "500.0000", "COM9-StopBits": "2", "405 AO-Description": "NI DAC", "COM4-DelayBetweenCharsMs": "0.0000", "445 AO-Block voltage": "false", "520 AO-TriggerInputLine": "/Dev1/PFI0", "555 AO-Description": "NI DAC", "520 AO-TriggerSampleRate": "100000", "COM4-Verbose": "1", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM4-Name": "COM4", "405 Shutter-Volts": "0.0000", "Vincent-D3-Port": "COM1", "PRIME-PP 4 MIN ADU AFFECTED": "200", "470 Shutter-MinVolts": "0.0000", "PRIME-PP 3 ENABLED": "Yes", "470 AO-Description": "NI DAC", "PRIME-Description": "PVCAM API device adapter", "520 Shutter-MaxVolts": "5.0000", "520 Shutter-Volts": "0.0000", "528 AO-Sequenceable": "1", "PRIME-Binning": "1x1", "555 Shutter-MaxVolts": "5.0000", "528 Shutter-TriggerSequenceLength": "1000", "405 Shutter-Block voltage": "false", "XLIGHT Spin motor-State": "1", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "Vincent-D3-Time to open (ms)": "35.0000", "Vincent-D3-Time to close (ms)": "35.0000", "445 AO-TriggerSampleRate": "100000", "640 Shutter-IOChannel": "Dev1/ao14", "555 AO-Sequenceable": "1", "520 AO-Name": "AnalogIO", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "470 AO-Block voltage": "false", "COM1-Verbose": "1", "COM9-AnswerTimeout": "10000.0000", "528 Shutter-Name": "AnalogIO", "PRIME-Trigger Timeout (secs)": "10", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "NextFrame": 0, "470 AO-TriggerSequenceLength": "1000", "PRIME-TransposeXY": "0", "445 AO-TriggerSequenceLength": "1000", "COM1-Name": "COM1", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "COM9-DataBits": "8", "COM1-AnswerTimeout": "500.0000", "Camera": "", "TE2000-Version": "V2.50-V2.50-V2.10", "528 Shutter-Volts": "0.0000", "XLIGHT Hub-Name": "XLIGHT Hub", "PIEZO-Description": "Ludl stage driver adapter", "PositionName": "Pos29", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSampleRate": "100000", "PRIME-FanSpeedSetpoint": "High", "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "405 AO-TriggerSampleRate": "100000", "470 Shutter-Block voltage": "false", "470-State": "0", "COM3-Parity": "None", "405 AO-Volts": "0.0000", "PRIME-TransposeMirrorY": "0", "PRIME-ChipName": "GS144BSI", "470 AO-Volts": "0.0000", "PRIME-TransposeMirrorX": "0", "445 Shutter-SupportsTriggering": "0", "470 Shutter-SupportsTriggering": "0", "XLIGHT Hub-Description": "XLIGHT hub", "PRIME-PreampOffLimit": "10000", "470 AO-Sequenceable": "1", "528 Shutter-MaxVolts": "5.0000", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "Core-XYStage": "XYStage", "470 AO-TriggerSampleRate": "100000", "PRIME-Timing-PreTriggerDelayNs": "24672000.0000", "528-DA Device": "528 Shutter", "Focus-Description": "Nikon TE2000 focus adapter", "FilterBlock-State": "5", "520 AO-Demo": "No", "COM3-DelayBetweenCharsMs": "0.0000", "470 Shutter-Volts": "0.0000", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "PRIME-PP 2 THRESHOLD": "125", "PRIME-PP 4 THRESHOLD": "75", "XYStage-Acceleration": "4.0000", "470 Shutter-Demo": "No", "PRIME-Name": "PMPCIECam00", "640-DA Device": "640 Shutter", "405 AO-Block voltage": "false", "ElapsedTime-ms": 1218581, "PRIME-Offset": "100", "445 AO-IOChannel": "Dev1/ao1", "640 AO-MaxVolts": "5.0000", "PositionIndex": 29, "PRIME-PP 1 ENABLED": "Yes", "COM1-BaudRate": "9600", "640-State": "0", "COM3-Verbose": "1", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "LudlController-TransmissionDelay": "0", "PRIME-FullWellCapacity": "62000", "PRIME-SMARTStreamingEnabled": "No", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "640 Shutter-Description": "NI DAC", "528-Description": "DA device that is used as a shutter", "COM4-Description": "Serial port driver (boost:asio)", "405 Shutter-Description": "NI DAC", "TE2000-Name": "TE2000", "528 Shutter-Description": "NI DAC", "COM9-Description": "Serial port driver (boost:asio)", "LudlController-Name": "LudlController", "445 Shutter-TriggerSequenceLength": "1000", "PRIME-SerialNumber": "A18B203008", "445 AO-Volts": "0.0000", "PRIME-X-dimension": "1200", "PRIME-ShutterCloseDelay": "0", "XLIGHT Disk slider-State": "2", "Position": "Default", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 AO-IOChannel": "Dev1/ao5", "520 AO-IOChannel": "Dev1/ao4", "528 AO-TriggerSequenceLength": "1000", "405 Shutter-MaxVolts": "5.0000", "470-DA Device": "470 Shutter", "PRIME-FTCapable": "No", "Core-ChannelGroup": "Channels", "555 AO-SupportsTriggering": "0", "PRIME-Y-dimension": "1200", "Frame": 0, "555 Shutter-Sequenceable": "1", "PRIME-Timing-ExposureTimeNs": "499998000.0000", "Core-ImageProcessor": "", "528-State": "0", "520 AO-Block voltage": "false", "XLIGHT Excitation Wheel-Label": "Excitation-2", "640 Shutter-Demo": "No", "COM3-StopBits": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "520 Shutter-IOChannel": "Dev1/ao11", "Width": 1200, "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-SupportsTriggering": "0", "640 AO-TriggerSampleRate": "100000", "520-Description": "DA device that is used as a shutter", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "405 Shutter-Sequenceable": "1", "405 Shutter-SupportsTriggering": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-TriggerSampleRate": "100000", "640 Shutter-MaxVolts": "5.0000", "555 AO-MaxVolts": "5.0000", "520 AO-TriggerSequenceLength": "1000", "COM3-BaudRate": "9600", "COM9-BaudRate": "9600", "Vincent-D3-Channel #": "Ch. #1", "PRIME-PMode": "Normal", "OpticalPath-Label": "Left(100)", "BitDepth": 16, "555 AO-TriggerSequenceLength": "1000", "445 Shutter-Description": "NI DAC", "405 AO-Sequenceable": "1", "PRIME-AcquisitionMethod": "Callbacks", "555 AO-Name": "AnalogIO", "PRIME-ShutterMode": "Pre-Exposure", "COM3-Handshaking": "Off", "445 AO-SupportsTriggering": "0", "ROI": "0-0-1200-1200", "COM9-Verbose": "1", "PRIME-Color": "OFF", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "PRIME-ShutterOpenDelay": "0", "COM1-Parity": "None", "Slice": 1, "SlicePosition": -40, "COM9-DelayBetweenCharsMs": "0.0000", "555-Name": "DA Shutter", "PIEZO-LudlSingleAxisName": "B", "XLIGHT Emission Wheel-Label": "Empty", "640 Shutter-Name": "AnalogIO", "640 AO-Sequenceable": "1", "520 Shutter-Demo": "No", "528 Shutter-Demo": "No", "470-Name": "DA Shutter", "520-DA Device": "520 Shutter", "XYStage-Description": "Ludl XY stage driver adapter", "445 Shutter-MinVolts": "0.0000", "528 AO-TriggerSampleRate": "100000", "405 AO-MinVolts": "0.0000", "405 Shutter-TriggerSequenceLength": "1000", "Height": 1200, "555 AO-Block voltage": "false", "640 Shutter-Volts": "0.0000", "PixelType": "GRAY16", "445 Shutter-Block voltage": "false", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "520 Shutter-TriggerSequenceLength": "1000", "COM9-Name": "COM9", "Core-SLM": "", "555 AO-Volts": "0.0000", "555 Shutter-Block voltage": "false", "XYStage-Speed": "1800.0000", "520 AO-MaxVolts": "5.0000", "528 Shutter-SupportsTriggering": "0", "COM1-Description": "Serial port driver (boost:asio)", "PRIME-MetadataEnabled": "No", "520 Shutter-MinVolts": "0.0000", "470 Shutter-Sequenceable": "1", "PRIME-PreampDelay": "0", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-MinVolts": "0.0000", "ChannelIndex": 4, "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Core-AutoShutter": "1", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "Exposure-ms": 20, "PRIME-PP 2 ENABLED": "Yes", "640 Shutter-TriggerSampleRate": "100000", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "470 Shutter-IOChannel": "Dev1/ao10", "405 AO-Demo": "No", "PixelSizeUm": 1.1, "470 AO-Demo": "No", "470 Shutter-MaxVolts": "5.0000", "Vincent-D3-Name": "Vincent-D3", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FileName": "img_000000000_P-DIC_001.tif", "LudlController-Reset": "Operate", "PRIME-Exposure": "20.00", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "445-Name": "DA Shutter", "555 Shutter-Name": "AnalogIO", "520 Shutter-TriggerSampleRate": "100000", "445 Shutter-IOChannel": "Dev1/ao9", "PRIME-TransposeCorrection": "0", "XYStage-StepSize": "0.1000", "445-Description": "DA device that is used as a shutter", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 AO-IOChannel": "Dev1/ao7", "520 Shutter-Name": "AnalogIO", "COM3-DataBits": "8", "640 AO-Volts": "0.0000", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "555 Shutter-IOChannel": "Dev1/ao13", "WaitInterval": null, "520 Shutter-Sequenceable": "1", "YPositionUm": -180, "445-State": "0", "445-DA Device": "445 Shutter", "PRIME-CircularBufferAutoSize": "ON", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "555 AO-TriggerSampleRate": "100000", "445 Shutter-Volts": "0.0000", "XLIGHT Hub-Port": "COM3", "Core-AutoFocus": "", "COM4-Handshaking": "Off", "528 Shutter-MinVolts": "0.0000", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "445 Shutter-Name": "AnalogIO", "528 Shutter-Sequenceable": "1", "XYStage-TransposeMirrorX": "0", "XYStage-TransposeMirrorY": "0", "PRIME-ReadoutRate": "100MHz 16bit", "XYStage-Name": "Stage", "640 AO-SupportsTriggering": "0", "640-Name": "DA Shutter", "640 Shutter-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "640 AO-Block voltage": "false", "445 Shutter-Sequenceable": "1", "470 AO-MaxVolts": "5.0000", "COM4-BaudRate": "9600", "470-Description": "DA device that is used as a shutter", "520 Shutter-Description": "NI DAC", "PRIME-CCDTemperature": "-14.8500", "Time": "2019-12-18 23:11:18 -0500", "640 AO-TriggerSequenceLength": "1000", "470 AO-Name": "AnalogIO", "XLIGHT Disk slider-Label": "Disk Pos 40um", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "Binning": "1x1", "TE2000-ModelType": "1", "COM3-AnswerTimeout": "500.0000", "555 Shutter-SupportsTriggering": "0", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "TE2000-Port": "COM4", "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Description": "NI DAC", "COM4-AnswerTimeout": "500.0000", "COM1-DelayBetweenCharsMs": "0.0000", "FilterBlock-Label": "Confocal", "445 AO-Name": "AnalogIO", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-Timing-ReadoutTimeNs": "24672000.0000", "528 AO-Demo": "No", "520 AO-Sequenceable": "1", "555-Description": "DA device that is used as a shutter", "405-DA Device": "405 Shutter", "405 AO-TriggerSequenceLength": "1000", "640 AO-MinVolts": "0.0000", "640 Shutter-Block voltage": "false", "FrameIndex": 0, "PRIME-Port": "CMOS", "555 AO-MinVolts": "0.0000", "COM3-Description": "Serial port driver (boost:asio)", "528 AO-Description": "NI DAC", "555 AO-IOChannel": "Dev1/ao6", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "555 AO-TriggerInputLine": "/Dev1/PFI0", "PRIME-ActualInterval-ms": "390.5200", "LudlController-Port": "COM9", "445 Shutter-Demo": "No", "PRIME-Trigger-Expose Out-Mux": "1", "SliceIndex": 1, "PRIME-CCDTemperatureSetPoint": "-15.0000", "405 Shutter-MinVolts": "0.0000", "PRIME-ExposeOutMode": "First Row", "AxisPositions": null, "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Volts": "0.0000", "528-Name": "DA Shutter", "555-State": "0", "XLIGHT Dichroic-Label": "Di03-R635-t1", "445 AO-Sequenceable": "1", "405-Name": "DA Shutter", "405 AO-IOChannel": "Dev1/ao0", "Core-Camera": "PRIME", "Focus-Name": "Focus", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "405 Shutter-IOChannel": "Dev1/ao8", "640 AO-Demo": "No", "640 Shutter-SupportsTriggering": "0", "445 AO-Demo": "No", "555-DA Device": "555 Shutter", "470 Shutter-Description": "NI DAC", "528 AO-Volts": "0.0000", "COM1-DataBits": "8", "Vincent-D3-Address": "x", "520 AO-SupportsTriggering": "0", "405-Description": "DA device that is used as a shutter", "640 Shutter-MinVolts": "0.0000", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "405 AO-Name": "AnalogIO", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "640 AO-TriggerInputLine": "/Dev1/PFI0", "405 AO-TriggerInputLine": "/Dev1/PFI0", "640-Description": "DA device that is used as a shutter", "445 Shutter-TriggerSampleRate": "100000", "470 Shutter-TriggerSequenceLength": "1000", "COM1-StopBits": "1", "520 Shutter-SupportsTriggering": "0", "405-State": "0", "Core-Initialize": "1", "XLIGHT Emission Wheel-State": "3", "520 AO-Description": "NI DAC", "528 AO-TriggerInputLine": "/Dev1/PFI0", "445 Shutter-MaxVolts": "5.0000", "COM9-Handshaking": "Off", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "XLIGHT Spin motor-Label": "On (spinning)", "PRIME-PP 5 ENABLED": "No", "UUID": "a9fa46d2-b873-47af-b553-2cba3a159dc5", "PRIME-TriggerMode": "Internal Trigger", "COM1-Handshaking": "Off", "PRIME-Timing-PostTriggerDelayNs": "49344000.0000", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "Focus-Position": "17741.5000", "528 Shutter-Block voltage": "false", "Channel": "P-DIC", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "555 Shutter-Demo": "No", "405 Shutter-Demo": "No", "528 AO-Name": "AnalogIO", "520-State": "0", "Core-Galvo": "", "405 Shutter-TriggerSampleRate": "100000", "Focus-FocusStepSize_(nm)": "100", "PRIME-PixelType": "16bit", "COM4-DataBits": "8", "470 AO-IOChannel": "Dev1/ao2", "520 AO-MinVolts": "0.0000", "COM9-Parity": "None", "PRIME-CircularBufferEnabled": "ON", "PRIME-PP 6 Reset": "No", "640 AO-Description": "NI DAC", "Source": "PRIME", "445 AO-MinVolts": "0.0000", "PIEZO-Name": "Stage", "445 AO-Description": "NI DAC", "PRIME-CircularBufferFrameCount": "46", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "520 AO-Volts": "0.0000", "528 Shutter-TriggerSampleRate": "100000", "XPositionUm": 15712.1, "OpticalPath-State": "4", "PRIME-PP 3 THRESHOLD": "80", "PRIME-PVCAM Version": "3.7.1", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Gain": "1-HDR", "640 Shutter-TriggerSequenceLength": "1000", "528 AO-SupportsTriggering": "0", "445 AO-TriggerInputLine": "/Dev1/PFI0", "OpticalPath-Name": "OpticalPath", "PRIME-PP 4 ENABLED": "Yes", "PRIME-PVCAM Adapter Version": "1.3.29", "COM3-Name": "COM3", "405 AO-MaxVolts": "5.0000", "520 Shutter-Block voltage": "false", "PRIME-Timing-ClearingTimeNs": "49344000.0000", "Core-Focus": "PIEZO", "528 AO-Block voltage": "false", "TE2000-Description": "Nikon TE2000 hub", "528 AO-MinVolts": "0.0000", "COM4-Parity": "None", "Summary": { "Slices": 11, "ChContrastMin": [ 0, 0, 0, 0, 0, 0 ], "PixelAspect": 1, "SlicesFirst": true, "Interval_ms": 0, "Time": "2019-12-18 23:10:50 -0500", "Prefix": "seq_5", "InitialPositionList": [ { "Label": "Pos0", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.21], "Focus": [17741.5], "XYStage": [ 44542.200000000004, 31636.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos1", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.22], "Focus": [17741.4], "XYStage": [ 43801.100000000006, 31637.9 ] }, "GridColumnIndex": 0 }, { "Label": "Pos2", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.5], "XYStage": [ 38561.3, 32040.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos3", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.185], "Focus": [17741.3], "XYStage": [ 37698.4, 32041.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos4", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.16], "Focus": [17741.5], "XYStage": [ 32375.4, 31758.9 ] }, "GridColumnIndex": 0 }, { "Label": "Pos5", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.17000000000002], "Focus": [17741.3], "XYStage": [ 31770.7, 31758.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos6", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.4], "XYStage": [ 26536.800000000003, 31068.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos7", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.205], "Focus": [17741.4], "XYStage": [ 25420.2, 31068.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos8", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.85], "Focus": [17741.3], "XYStage": [ 21059, 31508 ] }, "GridColumnIndex": 0 }, { "Label": "Pos9", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.82], "Focus": [17741.3], "XYStage": [ 19731.9, 31506.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos10", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.79], "Focus": [17741.4], "XYStage": [ 14528.5, 32007.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos11", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.83], "Focus": [17741.2], "XYStage": [ 13830.300000000001, 32009.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos12", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.79], "Focus": [17741.3], "XYStage": [ 8775.300000000001, 32017.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos13", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.815], "Focus": [17741.3], "XYStage": [ 8109.200000000001, 32008.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos14", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.805], "Focus": [17741.3], "XYStage": [ 3483.3, 31908.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos15", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.82], "Focus": [17741.2], "XYStage": [ 2686.8, 31903.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos16", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [211.48000000000002], "Focus": [17741.1], "XYStage": [ -1978.9, 31973.600000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos17", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [211.51500000000001], "Focus": [17741.1], "XYStage": [ -2957.8, 31982.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos18", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.195], "Focus": [17741.5], "XYStage": [ 47747.4, -576.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos19", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.23000000000002], "Focus": [17741.6], "XYStage": [ 47053.9, -576.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos20", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.205], "Focus": [17741.4], "XYStage": [ 41871.200000000004, -479.8 ] }, "GridColumnIndex": 0 }, { "Label": "Pos21", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.4], "XYStage": [ 40882.600000000006, -392 ] }, "GridColumnIndex": 0 }, { "Label": "Pos22", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.24], "Focus": [17741.4], "XYStage": [ 35905.6, -135.3 ] }, "GridColumnIndex": 0 }, { "Label": "Pos23", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.195], "Focus": [17741.5], "XYStage": [ 34990.9, -132.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos24", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.46], "Focus": [17741.4], "XYStage": [ 29678.100000000002, -958.1 ] }, "GridColumnIndex": 0 }, { "Label": "Pos25", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.44], "Focus": [17741.5], "XYStage": [ 28983.600000000002, -939.4000000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos26", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.475], "Focus": [17741.5], "XYStage": [ 23019.7, -122 ] }, "GridColumnIndex": 0 }, { "Label": "Pos27", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.44], "Focus": [17741.7], "XYStage": [ 22428, -116.80000000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos28", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.46], "Focus": [17741.7], "XYStage": [ 16364.900000000001, -181.10000000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos29", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.48000000000002], "Focus": [17741.5], "XYStage": [ 15712.1, -180 ] }, "GridColumnIndex": 0 }, { "Label": "Pos30", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.45000000000002], "Focus": [17741.7], "XYStage": [ 10346.1, 77.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos31", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.49], "Focus": [17741.4], "XYStage": [ 9755.5, 85.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos32", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.475], "Focus": [17741.5], "XYStage": [ 4775.5, 293.90000000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos33", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.445], "Focus": [17741.4], "XYStage": [ 3954.9, 293.90000000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos34", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.455], "Focus": [17741.4], "XYStage": [ -1324.9, 545.6 ] }, "GridColumnIndex": 0 }, { "Label": "Pos35", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.445], "Focus": [17741.3], "XYStage": [ -2097, 576.6 ] }, "GridColumnIndex": 0 } ], "z-step_um": 10, "GridRow": 0, "BitDepth": 16, "Depth": 2, "Channels": 6, "Source": "Micro-Manager", "ChColors": [ -16711681, -256, -65281, -1, -1, -16776961 ], "MetadataVersion": 10, "KeepShutterOpenChannels": true, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536, 65536 ], "CustomIntervals_ms": [], "Height": 1200, "MicroManagerVersion": "1.4.23 20180219", "GridColumn": 0, "UUID": "67598d21-5db5-4842-ab60-f506f7b42c7c", "PixelType": "GRAY16", "Width": 1200, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\Yan\\20191218-YC113-S7S8-miseq", "Frames": 1, "Comment": "", "UserName": "Nikon", "PixelSize_um": 1.1, "ROI": [ 0, 0, 1200, 1200 ], "Date": "2019-12-18", "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC", "P-DAPI-high" ], "KeepShutterOpenSlices": true, "IJType": 1, "ComputerName": "ZadorConfocal2", "Positions": 36, "PositionIndex": 29, "CameraTimeout": 20000, "TimeFirst": false }, "640 AO-Name": "AnalogIO", "Vincent-D3-Command": "Close" }, "FrameKey-0-4-2": { "COM4-StopBits": "1", "528 AO-MaxVolts": "5.0000", "XLIGHT Excitation Wheel-State": "1", "470 Shutter-Name": "AnalogIO", "Core-Shutter": "Vincent-D3", "555 AO-Demo": "No", "XLIGHT Dichroic-State": "2", "XYStage-StepSize-Y": "0.1000", "ZPositionUm": 188.48000000000002, "XYStage-StepSize-X": "0.1000", "PRIME-FirmwareVersion": "20.28", "PRIME-ClearCycles": "2", "520-Name": "DA Shutter", "405 Shutter-Name": "AnalogIO", "PIEZO-StepSize": "0.0050", "PRIME-ClearMode": "Pre-Exposure", "470 AO-MinVolts": "0.0000", "405 AO-SupportsTriggering": "1", "CameraChannelIndex": 0, "XYStage-StartSpeed": "500.0000", "COM9-StopBits": "2", "405 AO-Description": "NI DAC", "COM4-DelayBetweenCharsMs": "0.0000", "445 AO-Block voltage": "false", "520 AO-TriggerInputLine": "/Dev1/PFI0", "555 AO-Description": "NI DAC", "520 AO-TriggerSampleRate": "100000", "COM4-Verbose": "1", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM4-Name": "COM4", "405 Shutter-Volts": "0.0000", "Vincent-D3-Port": "COM1", "PRIME-PP 4 MIN ADU AFFECTED": "200", "470 Shutter-MinVolts": "0.0000", "PRIME-PP 3 ENABLED": "Yes", "470 AO-Description": "NI DAC", "PRIME-Description": "PVCAM API device adapter", "520 Shutter-MaxVolts": "5.0000", "520 Shutter-Volts": "0.0000", "528 AO-Sequenceable": "1", "PRIME-Binning": "1x1", "555 Shutter-MaxVolts": "5.0000", "528 Shutter-TriggerSequenceLength": "1000", "405 Shutter-Block voltage": "false", "XLIGHT Spin motor-State": "1", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "Vincent-D3-Time to open (ms)": "35.0000", "Vincent-D3-Time to close (ms)": "35.0000", "445 AO-TriggerSampleRate": "100000", "640 Shutter-IOChannel": "Dev1/ao14", "555 AO-Sequenceable": "1", "520 AO-Name": "AnalogIO", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "470 AO-Block voltage": "false", "COM1-Verbose": "1", "COM9-AnswerTimeout": "10000.0000", "528 Shutter-Name": "AnalogIO", "PRIME-Trigger Timeout (secs)": "10", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "NextFrame": 0, "470 AO-TriggerSequenceLength": "1000", "PRIME-TransposeXY": "0", "445 AO-TriggerSequenceLength": "1000", "COM1-Name": "COM1", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "COM9-DataBits": "8", "COM1-AnswerTimeout": "500.0000", "Camera": "", "TE2000-Version": "V2.50-V2.50-V2.10", "528 Shutter-Volts": "0.0000", "XLIGHT Hub-Name": "XLIGHT Hub", "PIEZO-Description": "Ludl stage driver adapter", "PositionName": "Pos29", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSampleRate": "100000", "PRIME-FanSpeedSetpoint": "High", "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "405 AO-TriggerSampleRate": "100000", "470 Shutter-Block voltage": "false", "470-State": "0", "COM3-Parity": "None", "405 AO-Volts": "0.0000", "PRIME-TransposeMirrorY": "0", "PRIME-ChipName": "GS144BSI", "470 AO-Volts": "0.0000", "PRIME-TransposeMirrorX": "0", "445 Shutter-SupportsTriggering": "0", "470 Shutter-SupportsTriggering": "0", "XLIGHT Hub-Description": "XLIGHT hub", "PRIME-PreampOffLimit": "10000", "470 AO-Sequenceable": "1", "528 Shutter-MaxVolts": "5.0000", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "Core-XYStage": "XYStage", "470 AO-TriggerSampleRate": "100000", "PRIME-Timing-PreTriggerDelayNs": "24672000.0000", "528-DA Device": "528 Shutter", "Focus-Description": "Nikon TE2000 focus adapter", "FilterBlock-State": "5", "520 AO-Demo": "No", "COM3-DelayBetweenCharsMs": "0.0000", "470 Shutter-Volts": "0.0000", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "PRIME-PP 2 THRESHOLD": "125", "PRIME-PP 4 THRESHOLD": "75", "XYStage-Acceleration": "4.0000", "470 Shutter-Demo": "No", "PRIME-Name": "PMPCIECam00", "640-DA Device": "640 Shutter", "405 AO-Block voltage": "false", "ElapsedTime-ms": 1218838, "PRIME-Offset": "100", "445 AO-IOChannel": "Dev1/ao1", "640 AO-MaxVolts": "5.0000", "PositionIndex": 29, "PRIME-PP 1 ENABLED": "Yes", "COM1-BaudRate": "9600", "640-State": "0", "COM3-Verbose": "1", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "LudlController-TransmissionDelay": "0", "PRIME-FullWellCapacity": "62000", "PRIME-SMARTStreamingEnabled": "No", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "640 Shutter-Description": "NI DAC", "528-Description": "DA device that is used as a shutter", "COM4-Description": "Serial port driver (boost:asio)", "405 Shutter-Description": "NI DAC", "TE2000-Name": "TE2000", "528 Shutter-Description": "NI DAC", "COM9-Description": "Serial port driver (boost:asio)", "LudlController-Name": "LudlController", "445 Shutter-TriggerSequenceLength": "1000", "PRIME-SerialNumber": "A18B203008", "445 AO-Volts": "0.0000", "PRIME-X-dimension": "1200", "PRIME-ShutterCloseDelay": "0", "XLIGHT Disk slider-State": "2", "Position": "Default", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 AO-IOChannel": "Dev1/ao5", "520 AO-IOChannel": "Dev1/ao4", "528 AO-TriggerSequenceLength": "1000", "405 Shutter-MaxVolts": "5.0000", "470-DA Device": "470 Shutter", "PRIME-FTCapable": "No", "Core-ChannelGroup": "Channels", "555 AO-SupportsTriggering": "0", "PRIME-Y-dimension": "1200", "Frame": 0, "555 Shutter-Sequenceable": "1", "PRIME-Timing-ExposureTimeNs": "499998000.0000", "Core-ImageProcessor": "", "528-State": "0", "520 AO-Block voltage": "false", "XLIGHT Excitation Wheel-Label": "Excitation-2", "640 Shutter-Demo": "No", "COM3-StopBits": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "520 Shutter-IOChannel": "Dev1/ao11", "Width": 1200, "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-SupportsTriggering": "0", "640 AO-TriggerSampleRate": "100000", "520-Description": "DA device that is used as a shutter", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "405 Shutter-Sequenceable": "1", "405 Shutter-SupportsTriggering": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-TriggerSampleRate": "100000", "640 Shutter-MaxVolts": "5.0000", "555 AO-MaxVolts": "5.0000", "520 AO-TriggerSequenceLength": "1000", "COM3-BaudRate": "9600", "COM9-BaudRate": "9600", "Vincent-D3-Channel #": "Ch. #1", "PRIME-PMode": "Normal", "OpticalPath-Label": "Left(100)", "BitDepth": 16, "555 AO-TriggerSequenceLength": "1000", "445 Shutter-Description": "NI DAC", "405 AO-Sequenceable": "1", "PRIME-AcquisitionMethod": "Callbacks", "555 AO-Name": "AnalogIO", "PRIME-ShutterMode": "Pre-Exposure", "COM3-Handshaking": "Off", "445 AO-SupportsTriggering": "0", "ROI": "0-0-1200-1200", "COM9-Verbose": "1", "PRIME-Color": "OFF", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "PRIME-ShutterOpenDelay": "0", "COM1-Parity": "None", "Slice": 2, "SlicePosition": -30, "COM9-DelayBetweenCharsMs": "0.0000", "555-Name": "DA Shutter", "PIEZO-LudlSingleAxisName": "B", "XLIGHT Emission Wheel-Label": "Empty", "640 Shutter-Name": "AnalogIO", "640 AO-Sequenceable": "1", "520 Shutter-Demo": "No", "528 Shutter-Demo": "No", "470-Name": "DA Shutter", "520-DA Device": "520 Shutter", "XYStage-Description": "Ludl XY stage driver adapter", "445 Shutter-MinVolts": "0.0000", "528 AO-TriggerSampleRate": "100000", "405 AO-MinVolts": "0.0000", "405 Shutter-TriggerSequenceLength": "1000", "Height": 1200, "555 AO-Block voltage": "false", "640 Shutter-Volts": "0.0000", "PixelType": "GRAY16", "445 Shutter-Block voltage": "false", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "520 Shutter-TriggerSequenceLength": "1000", "COM9-Name": "COM9", "Core-SLM": "", "555 AO-Volts": "0.0000", "555 Shutter-Block voltage": "false", "XYStage-Speed": "1800.0000", "520 AO-MaxVolts": "5.0000", "528 Shutter-SupportsTriggering": "0", "COM1-Description": "Serial port driver (boost:asio)", "PRIME-MetadataEnabled": "No", "520 Shutter-MinVolts": "0.0000", "470 Shutter-Sequenceable": "1", "PRIME-PreampDelay": "0", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-MinVolts": "0.0000", "ChannelIndex": 4, "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Core-AutoShutter": "1", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "Exposure-ms": 20, "PRIME-PP 2 ENABLED": "Yes", "640 Shutter-TriggerSampleRate": "100000", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "470 Shutter-IOChannel": "Dev1/ao10", "405 AO-Demo": "No", "PixelSizeUm": 1.1, "470 AO-Demo": "No", "470 Shutter-MaxVolts": "5.0000", "Vincent-D3-Name": "Vincent-D3", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FileName": "img_000000000_P-DIC_002.tif", "LudlController-Reset": "Operate", "PRIME-Exposure": "20.00", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "445-Name": "DA Shutter", "555 Shutter-Name": "AnalogIO", "520 Shutter-TriggerSampleRate": "100000", "445 Shutter-IOChannel": "Dev1/ao9", "PRIME-TransposeCorrection": "0", "XYStage-StepSize": "0.1000", "445-Description": "DA device that is used as a shutter", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 AO-IOChannel": "Dev1/ao7", "520 Shutter-Name": "AnalogIO", "COM3-DataBits": "8", "640 AO-Volts": "0.0000", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "555 Shutter-IOChannel": "Dev1/ao13", "WaitInterval": null, "520 Shutter-Sequenceable": "1", "YPositionUm": -180, "445-State": "0", "445-DA Device": "445 Shutter", "PRIME-CircularBufferAutoSize": "ON", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "555 AO-TriggerSampleRate": "100000", "445 Shutter-Volts": "0.0000", "XLIGHT Hub-Port": "COM3", "Core-AutoFocus": "", "COM4-Handshaking": "Off", "528 Shutter-MinVolts": "0.0000", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "445 Shutter-Name": "AnalogIO", "528 Shutter-Sequenceable": "1", "XYStage-TransposeMirrorX": "0", "XYStage-TransposeMirrorY": "0", "PRIME-ReadoutRate": "100MHz 16bit", "XYStage-Name": "Stage", "640 AO-SupportsTriggering": "0", "640-Name": "DA Shutter", "640 Shutter-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "640 AO-Block voltage": "false", "445 Shutter-Sequenceable": "1", "470 AO-MaxVolts": "5.0000", "COM4-BaudRate": "9600", "470-Description": "DA device that is used as a shutter", "520 Shutter-Description": "NI DAC", "PRIME-CCDTemperature": "-14.8500", "Time": "2019-12-18 23:11:19 -0500", "640 AO-TriggerSequenceLength": "1000", "470 AO-Name": "AnalogIO", "XLIGHT Disk slider-Label": "Disk Pos 40um", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "Binning": "1x1", "TE2000-ModelType": "1", "COM3-AnswerTimeout": "500.0000", "555 Shutter-SupportsTriggering": "0", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "TE2000-Port": "COM4", "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Description": "NI DAC", "COM4-AnswerTimeout": "500.0000", "COM1-DelayBetweenCharsMs": "0.0000", "FilterBlock-Label": "Confocal", "445 AO-Name": "AnalogIO", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-Timing-ReadoutTimeNs": "24672000.0000", "528 AO-Demo": "No", "520 AO-Sequenceable": "1", "555-Description": "DA device that is used as a shutter", "405-DA Device": "405 Shutter", "405 AO-TriggerSequenceLength": "1000", "640 AO-MinVolts": "0.0000", "640 Shutter-Block voltage": "false", "FrameIndex": 0, "PRIME-Port": "CMOS", "555 AO-MinVolts": "0.0000", "COM3-Description": "Serial port driver (boost:asio)", "528 AO-Description": "NI DAC", "555 AO-IOChannel": "Dev1/ao6", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "555 AO-TriggerInputLine": "/Dev1/PFI0", "PRIME-ActualInterval-ms": "390.5200", "LudlController-Port": "COM9", "445 Shutter-Demo": "No", "PRIME-Trigger-Expose Out-Mux": "1", "SliceIndex": 2, "PRIME-CCDTemperatureSetPoint": "-15.0000", "405 Shutter-MinVolts": "0.0000", "PRIME-ExposeOutMode": "First Row", "AxisPositions": null, "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Volts": "0.0000", "528-Name": "DA Shutter", "555-State": "0", "XLIGHT Dichroic-Label": "Di03-R635-t1", "445 AO-Sequenceable": "1", "405-Name": "DA Shutter", "405 AO-IOChannel": "Dev1/ao0", "Core-Camera": "PRIME", "Focus-Name": "Focus", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "405 Shutter-IOChannel": "Dev1/ao8", "640 AO-Demo": "No", "640 Shutter-SupportsTriggering": "0", "445 AO-Demo": "No", "555-DA Device": "555 Shutter", "470 Shutter-Description": "NI DAC", "528 AO-Volts": "0.0000", "COM1-DataBits": "8", "Vincent-D3-Address": "x", "520 AO-SupportsTriggering": "0", "405-Description": "DA device that is used as a shutter", "640 Shutter-MinVolts": "0.0000", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "405 AO-Name": "AnalogIO", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "640 AO-TriggerInputLine": "/Dev1/PFI0", "405 AO-TriggerInputLine": "/Dev1/PFI0", "640-Description": "DA device that is used as a shutter", "445 Shutter-TriggerSampleRate": "100000", "470 Shutter-TriggerSequenceLength": "1000", "COM1-StopBits": "1", "520 Shutter-SupportsTriggering": "0", "405-State": "0", "Core-Initialize": "1", "XLIGHT Emission Wheel-State": "3", "520 AO-Description": "NI DAC", "528 AO-TriggerInputLine": "/Dev1/PFI0", "445 Shutter-MaxVolts": "5.0000", "COM9-Handshaking": "Off", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "XLIGHT Spin motor-Label": "On (spinning)", "PRIME-PP 5 ENABLED": "No", "UUID": "802d51c0-8579-4270-85a9-04b22183bdc6", "PRIME-TriggerMode": "Internal Trigger", "COM1-Handshaking": "Off", "PRIME-Timing-PostTriggerDelayNs": "49344000.0000", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "Focus-Position": "17741.5000", "528 Shutter-Block voltage": "false", "Channel": "P-DIC", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "555 Shutter-Demo": "No", "405 Shutter-Demo": "No", "528 AO-Name": "AnalogIO", "520-State": "0", "Core-Galvo": "", "405 Shutter-TriggerSampleRate": "100000", "Focus-FocusStepSize_(nm)": "100", "PRIME-PixelType": "16bit", "COM4-DataBits": "8", "470 AO-IOChannel": "Dev1/ao2", "520 AO-MinVolts": "0.0000", "COM9-Parity": "None", "PRIME-CircularBufferEnabled": "ON", "PRIME-PP 6 Reset": "No", "640 AO-Description": "NI DAC", "Source": "PRIME", "445 AO-MinVolts": "0.0000", "PIEZO-Name": "Stage", "445 AO-Description": "NI DAC", "PRIME-CircularBufferFrameCount": "46", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "520 AO-Volts": "0.0000", "528 Shutter-TriggerSampleRate": "100000", "XPositionUm": 15712.1, "OpticalPath-State": "4", "PRIME-PP 3 THRESHOLD": "80", "PRIME-PVCAM Version": "3.7.1", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Gain": "1-HDR", "640 Shutter-TriggerSequenceLength": "1000", "528 AO-SupportsTriggering": "0", "445 AO-TriggerInputLine": "/Dev1/PFI0", "OpticalPath-Name": "OpticalPath", "PRIME-PP 4 ENABLED": "Yes", "PRIME-PVCAM Adapter Version": "1.3.29", "COM3-Name": "COM3", "405 AO-MaxVolts": "5.0000", "520 Shutter-Block voltage": "false", "PRIME-Timing-ClearingTimeNs": "49344000.0000", "Core-Focus": "PIEZO", "528 AO-Block voltage": "false", "TE2000-Description": "Nikon TE2000 hub", "528 AO-MinVolts": "0.0000", "COM4-Parity": "None", "Summary": { "Slices": 11, "ChContrastMin": [ 0, 0, 0, 0, 0, 0 ], "PixelAspect": 1, "SlicesFirst": true, "Interval_ms": 0, "Time": "2019-12-18 23:10:50 -0500", "Prefix": "seq_5", "InitialPositionList": [ { "Label": "Pos0", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.21], "Focus": [17741.5], "XYStage": [ 44542.200000000004, 31636.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos1", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.22], "Focus": [17741.4], "XYStage": [ 43801.100000000006, 31637.9 ] }, "GridColumnIndex": 0 }, { "Label": "Pos2", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.5], "XYStage": [ 38561.3, 32040.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos3", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.185], "Focus": [17741.3], "XYStage": [ 37698.4, 32041.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos4", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.16], "Focus": [17741.5], "XYStage": [ 32375.4, 31758.9 ] }, "GridColumnIndex": 0 }, { "Label": "Pos5", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.17000000000002], "Focus": [17741.3], "XYStage": [ 31770.7, 31758.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos6", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.4], "XYStage": [ 26536.800000000003, 31068.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos7", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.205], "Focus": [17741.4], "XYStage": [ 25420.2, 31068.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos8", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.85], "Focus": [17741.3], "XYStage": [ 21059, 31508 ] }, "GridColumnIndex": 0 }, { "Label": "Pos9", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.82], "Focus": [17741.3], "XYStage": [ 19731.9, 31506.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos10", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.79], "Focus": [17741.4], "XYStage": [ 14528.5, 32007.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos11", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.83], "Focus": [17741.2], "XYStage": [ 13830.300000000001, 32009.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos12", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.79], "Focus": [17741.3], "XYStage": [ 8775.300000000001, 32017.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos13", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.815], "Focus": [17741.3], "XYStage": [ 8109.200000000001, 32008.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos14", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.805], "Focus": [17741.3], "XYStage": [ 3483.3, 31908.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos15", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.82], "Focus": [17741.2], "XYStage": [ 2686.8, 31903.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos16", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [211.48000000000002], "Focus": [17741.1], "XYStage": [ -1978.9, 31973.600000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos17", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [211.51500000000001], "Focus": [17741.1], "XYStage": [ -2957.8, 31982.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos18", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.195], "Focus": [17741.5], "XYStage": [ 47747.4, -576.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos19", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.23000000000002], "Focus": [17741.6], "XYStage": [ 47053.9, -576.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos20", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.205], "Focus": [17741.4], "XYStage": [ 41871.200000000004, -479.8 ] }, "GridColumnIndex": 0 }, { "Label": "Pos21", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.4], "XYStage": [ 40882.600000000006, -392 ] }, "GridColumnIndex": 0 }, { "Label": "Pos22", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.24], "Focus": [17741.4], "XYStage": [ 35905.6, -135.3 ] }, "GridColumnIndex": 0 }, { "Label": "Pos23", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.195], "Focus": [17741.5], "XYStage": [ 34990.9, -132.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos24", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.46], "Focus": [17741.4], "XYStage": [ 29678.100000000002, -958.1 ] }, "GridColumnIndex": 0 }, { "Label": "Pos25", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.44], "Focus": [17741.5], "XYStage": [ 28983.600000000002, -939.4000000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos26", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.475], "Focus": [17741.5], "XYStage": [ 23019.7, -122 ] }, "GridColumnIndex": 0 }, { "Label": "Pos27", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.44], "Focus": [17741.7], "XYStage": [ 22428, -116.80000000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos28", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.46], "Focus": [17741.7], "XYStage": [ 16364.900000000001, -181.10000000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos29", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.48000000000002], "Focus": [17741.5], "XYStage": [ 15712.1, -180 ] }, "GridColumnIndex": 0 }, { "Label": "Pos30", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.45000000000002], "Focus": [17741.7], "XYStage": [ 10346.1, 77.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos31", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.49], "Focus": [17741.4], "XYStage": [ 9755.5, 85.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos32", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.475], "Focus": [17741.5], "XYStage": [ 4775.5, 293.90000000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos33", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.445], "Focus": [17741.4], "XYStage": [ 3954.9, 293.90000000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos34", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.455], "Focus": [17741.4], "XYStage": [ -1324.9, 545.6 ] }, "GridColumnIndex": 0 }, { "Label": "Pos35", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.445], "Focus": [17741.3], "XYStage": [ -2097, 576.6 ] }, "GridColumnIndex": 0 } ], "z-step_um": 10, "GridRow": 0, "BitDepth": 16, "Depth": 2, "Channels": 6, "Source": "Micro-Manager", "ChColors": [ -16711681, -256, -65281, -1, -1, -16776961 ], "MetadataVersion": 10, "KeepShutterOpenChannels": true, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536, 65536 ], "CustomIntervals_ms": [], "Height": 1200, "MicroManagerVersion": "1.4.23 20180219", "GridColumn": 0, "UUID": "67598d21-5db5-4842-ab60-f506f7b42c7c", "PixelType": "GRAY16", "Width": 1200, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\Yan\\20191218-YC113-S7S8-miseq", "Frames": 1, "Comment": "", "UserName": "Nikon", "PixelSize_um": 1.1, "ROI": [ 0, 0, 1200, 1200 ], "Date": "2019-12-18", "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC", "P-DAPI-high" ], "KeepShutterOpenSlices": true, "IJType": 1, "ComputerName": "ZadorConfocal2", "Positions": 36, "PositionIndex": 29, "CameraTimeout": 20000, "TimeFirst": false }, "640 AO-Name": "AnalogIO", "Vincent-D3-Command": "Close" }, "FrameKey-0-4-3": { "COM4-StopBits": "1", "528 AO-MaxVolts": "5.0000", "XLIGHT Excitation Wheel-State": "1", "470 Shutter-Name": "AnalogIO", "Core-Shutter": "Vincent-D3", "555 AO-Demo": "No", "XLIGHT Dichroic-State": "2", "XYStage-StepSize-Y": "0.1000", "ZPositionUm": 198.48000000000002, "XYStage-StepSize-X": "0.1000", "PRIME-FirmwareVersion": "20.28", "PRIME-ClearCycles": "2", "520-Name": "DA Shutter", "405 Shutter-Name": "AnalogIO", "PIEZO-StepSize": "0.0050", "PRIME-ClearMode": "Pre-Exposure", "470 AO-MinVolts": "0.0000", "405 AO-SupportsTriggering": "1", "CameraChannelIndex": 0, "XYStage-StartSpeed": "500.0000", "COM9-StopBits": "2", "405 AO-Description": "NI DAC", "COM4-DelayBetweenCharsMs": "0.0000", "445 AO-Block voltage": "false", "520 AO-TriggerInputLine": "/Dev1/PFI0", "555 AO-Description": "NI DAC", "520 AO-TriggerSampleRate": "100000", "COM4-Verbose": "1", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM4-Name": "COM4", "405 Shutter-Volts": "0.0000", "Vincent-D3-Port": "COM1", "PRIME-PP 4 MIN ADU AFFECTED": "200", "470 Shutter-MinVolts": "0.0000", "PRIME-PP 3 ENABLED": "Yes", "470 AO-Description": "NI DAC", "PRIME-Description": "PVCAM API device adapter", "520 Shutter-MaxVolts": "5.0000", "520 Shutter-Volts": "0.0000", "528 AO-Sequenceable": "1", "PRIME-Binning": "1x1", "555 Shutter-MaxVolts": "5.0000", "528 Shutter-TriggerSequenceLength": "1000", "405 Shutter-Block voltage": "false", "XLIGHT Spin motor-State": "1", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "Vincent-D3-Time to open (ms)": "35.0000", "Vincent-D3-Time to close (ms)": "35.0000", "445 AO-TriggerSampleRate": "100000", "640 Shutter-IOChannel": "Dev1/ao14", "555 AO-Sequenceable": "1", "520 AO-Name": "AnalogIO", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "470 AO-Block voltage": "false", "COM1-Verbose": "1", "COM9-AnswerTimeout": "10000.0000", "528 Shutter-Name": "AnalogIO", "PRIME-Trigger Timeout (secs)": "10", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "NextFrame": 0, "470 AO-TriggerSequenceLength": "1000", "PRIME-TransposeXY": "0", "445 AO-TriggerSequenceLength": "1000", "COM1-Name": "COM1", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "COM9-DataBits": "8", "COM1-AnswerTimeout": "500.0000", "Camera": "", "TE2000-Version": "V2.50-V2.50-V2.10", "528 Shutter-Volts": "0.0000", "XLIGHT Hub-Name": "XLIGHT Hub", "PIEZO-Description": "Ludl stage driver adapter", "PositionName": "Pos29", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSampleRate": "100000", "PRIME-FanSpeedSetpoint": "High", "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "405 AO-TriggerSampleRate": "100000", "470 Shutter-Block voltage": "false", "470-State": "0", "COM3-Parity": "None", "405 AO-Volts": "0.0000", "PRIME-TransposeMirrorY": "0", "PRIME-ChipName": "GS144BSI", "470 AO-Volts": "0.0000", "PRIME-TransposeMirrorX": "0", "445 Shutter-SupportsTriggering": "0", "470 Shutter-SupportsTriggering": "0", "XLIGHT Hub-Description": "XLIGHT hub", "PRIME-PreampOffLimit": "10000", "470 AO-Sequenceable": "1", "528 Shutter-MaxVolts": "5.0000", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "Core-XYStage": "XYStage", "470 AO-TriggerSampleRate": "100000", "PRIME-Timing-PreTriggerDelayNs": "24672000.0000", "528-DA Device": "528 Shutter", "Focus-Description": "Nikon TE2000 focus adapter", "FilterBlock-State": "5", "520 AO-Demo": "No", "COM3-DelayBetweenCharsMs": "0.0000", "470 Shutter-Volts": "0.0000", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "PRIME-PP 2 THRESHOLD": "125", "PRIME-PP 4 THRESHOLD": "75", "XYStage-Acceleration": "4.0000", "470 Shutter-Demo": "No", "PRIME-Name": "PMPCIECam00", "640-DA Device": "640 Shutter", "405 AO-Block voltage": "false", "ElapsedTime-ms": 1219099, "PRIME-Offset": "100", "445 AO-IOChannel": "Dev1/ao1", "640 AO-MaxVolts": "5.0000", "PositionIndex": 29, "PRIME-PP 1 ENABLED": "Yes", "COM1-BaudRate": "9600", "640-State": "0", "COM3-Verbose": "1", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "LudlController-TransmissionDelay": "0", "PRIME-FullWellCapacity": "62000", "PRIME-SMARTStreamingEnabled": "No", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "640 Shutter-Description": "NI DAC", "528-Description": "DA device that is used as a shutter", "COM4-Description": "Serial port driver (boost:asio)", "405 Shutter-Description": "NI DAC", "TE2000-Name": "TE2000", "528 Shutter-Description": "NI DAC", "COM9-Description": "Serial port driver (boost:asio)", "LudlController-Name": "LudlController", "445 Shutter-TriggerSequenceLength": "1000", "PRIME-SerialNumber": "A18B203008", "445 AO-Volts": "0.0000", "PRIME-X-dimension": "1200", "PRIME-ShutterCloseDelay": "0", "XLIGHT Disk slider-State": "2", "Position": "Default", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 AO-IOChannel": "Dev1/ao5", "520 AO-IOChannel": "Dev1/ao4", "528 AO-TriggerSequenceLength": "1000", "405 Shutter-MaxVolts": "5.0000", "470-DA Device": "470 Shutter", "PRIME-FTCapable": "No", "Core-ChannelGroup": "Channels", "555 AO-SupportsTriggering": "0", "PRIME-Y-dimension": "1200", "Frame": 0, "555 Shutter-Sequenceable": "1", "PRIME-Timing-ExposureTimeNs": "499998000.0000", "Core-ImageProcessor": "", "528-State": "0", "520 AO-Block voltage": "false", "XLIGHT Excitation Wheel-Label": "Excitation-2", "640 Shutter-Demo": "No", "COM3-StopBits": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "520 Shutter-IOChannel": "Dev1/ao11", "Width": 1200, "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-SupportsTriggering": "0", "640 AO-TriggerSampleRate": "100000", "520-Description": "DA device that is used as a shutter", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "405 Shutter-Sequenceable": "1", "405 Shutter-SupportsTriggering": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-TriggerSampleRate": "100000", "640 Shutter-MaxVolts": "5.0000", "555 AO-MaxVolts": "5.0000", "520 AO-TriggerSequenceLength": "1000", "COM3-BaudRate": "9600", "COM9-BaudRate": "9600", "Vincent-D3-Channel #": "Ch. #1", "PRIME-PMode": "Normal", "OpticalPath-Label": "Left(100)", "BitDepth": 16, "555 AO-TriggerSequenceLength": "1000", "445 Shutter-Description": "NI DAC", "405 AO-Sequenceable": "1", "PRIME-AcquisitionMethod": "Callbacks", "555 AO-Name": "AnalogIO", "PRIME-ShutterMode": "Pre-Exposure", "COM3-Handshaking": "Off", "445 AO-SupportsTriggering": "0", "ROI": "0-0-1200-1200", "COM9-Verbose": "1", "PRIME-Color": "OFF", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "PRIME-ShutterOpenDelay": "0", "COM1-Parity": "None", "Slice": 3, "SlicePosition": -20, "COM9-DelayBetweenCharsMs": "0.0000", "555-Name": "DA Shutter", "PIEZO-LudlSingleAxisName": "B", "XLIGHT Emission Wheel-Label": "Empty", "640 Shutter-Name": "AnalogIO", "640 AO-Sequenceable": "1", "520 Shutter-Demo": "No", "528 Shutter-Demo": "No", "470-Name": "DA Shutter", "520-DA Device": "520 Shutter", "XYStage-Description": "Ludl XY stage driver adapter", "445 Shutter-MinVolts": "0.0000", "528 AO-TriggerSampleRate": "100000", "405 AO-MinVolts": "0.0000", "405 Shutter-TriggerSequenceLength": "1000", "Height": 1200, "555 AO-Block voltage": "false", "640 Shutter-Volts": "0.0000", "PixelType": "GRAY16", "445 Shutter-Block voltage": "false", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "520 Shutter-TriggerSequenceLength": "1000", "COM9-Name": "COM9", "Core-SLM": "", "555 AO-Volts": "0.0000", "555 Shutter-Block voltage": "false", "XYStage-Speed": "1800.0000", "520 AO-MaxVolts": "5.0000", "528 Shutter-SupportsTriggering": "0", "COM1-Description": "Serial port driver (boost:asio)", "PRIME-MetadataEnabled": "No", "520 Shutter-MinVolts": "0.0000", "470 Shutter-Sequenceable": "1", "PRIME-PreampDelay": "0", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-MinVolts": "0.0000", "ChannelIndex": 4, "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Core-AutoShutter": "1", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "Exposure-ms": 20, "PRIME-PP 2 ENABLED": "Yes", "640 Shutter-TriggerSampleRate": "100000", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "470 Shutter-IOChannel": "Dev1/ao10", "405 AO-Demo": "No", "PixelSizeUm": 1.1, "470 AO-Demo": "No", "470 Shutter-MaxVolts": "5.0000", "Vincent-D3-Name": "Vincent-D3", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FileName": "img_000000000_P-DIC_003.tif", "LudlController-Reset": "Operate", "PRIME-Exposure": "20.00", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "445-Name": "DA Shutter", "555 Shutter-Name": "AnalogIO", "520 Shutter-TriggerSampleRate": "100000", "445 Shutter-IOChannel": "Dev1/ao9", "PRIME-TransposeCorrection": "0", "XYStage-StepSize": "0.1000", "445-Description": "DA device that is used as a shutter", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 AO-IOChannel": "Dev1/ao7", "520 Shutter-Name": "AnalogIO", "COM3-DataBits": "8", "640 AO-Volts": "0.0000", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "555 Shutter-IOChannel": "Dev1/ao13", "WaitInterval": null, "520 Shutter-Sequenceable": "1", "YPositionUm": -180, "445-State": "0", "445-DA Device": "445 Shutter", "PRIME-CircularBufferAutoSize": "ON", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "555 AO-TriggerSampleRate": "100000", "445 Shutter-Volts": "0.0000", "XLIGHT Hub-Port": "COM3", "Core-AutoFocus": "", "COM4-Handshaking": "Off", "528 Shutter-MinVolts": "0.0000", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "445 Shutter-Name": "AnalogIO", "528 Shutter-Sequenceable": "1", "XYStage-TransposeMirrorX": "0", "XYStage-TransposeMirrorY": "0", "PRIME-ReadoutRate": "100MHz 16bit", "XYStage-Name": "Stage", "640 AO-SupportsTriggering": "0", "640-Name": "DA Shutter", "640 Shutter-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "640 AO-Block voltage": "false", "445 Shutter-Sequenceable": "1", "470 AO-MaxVolts": "5.0000", "COM4-BaudRate": "9600", "470-Description": "DA device that is used as a shutter", "520 Shutter-Description": "NI DAC", "PRIME-CCDTemperature": "-14.8500", "Time": "2019-12-18 23:11:19 -0500", "640 AO-TriggerSequenceLength": "1000", "470 AO-Name": "AnalogIO", "XLIGHT Disk slider-Label": "Disk Pos 40um", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "Binning": "1x1", "TE2000-ModelType": "1", "COM3-AnswerTimeout": "500.0000", "555 Shutter-SupportsTriggering": "0", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "TE2000-Port": "COM4", "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Description": "NI DAC", "COM4-AnswerTimeout": "500.0000", "COM1-DelayBetweenCharsMs": "0.0000", "FilterBlock-Label": "Confocal", "445 AO-Name": "AnalogIO", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-Timing-ReadoutTimeNs": "24672000.0000", "528 AO-Demo": "No", "520 AO-Sequenceable": "1", "555-Description": "DA device that is used as a shutter", "405-DA Device": "405 Shutter", "405 AO-TriggerSequenceLength": "1000", "640 AO-MinVolts": "0.0000", "640 Shutter-Block voltage": "false", "FrameIndex": 0, "PRIME-Port": "CMOS", "555 AO-MinVolts": "0.0000", "COM3-Description": "Serial port driver (boost:asio)", "528 AO-Description": "NI DAC", "555 AO-IOChannel": "Dev1/ao6", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "555 AO-TriggerInputLine": "/Dev1/PFI0", "PRIME-ActualInterval-ms": "390.5200", "LudlController-Port": "COM9", "445 Shutter-Demo": "No", "PRIME-Trigger-Expose Out-Mux": "1", "SliceIndex": 3, "PRIME-CCDTemperatureSetPoint": "-15.0000", "405 Shutter-MinVolts": "0.0000", "PRIME-ExposeOutMode": "First Row", "AxisPositions": null, "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Volts": "0.0000", "528-Name": "DA Shutter", "555-State": "0", "XLIGHT Dichroic-Label": "Di03-R635-t1", "445 AO-Sequenceable": "1", "405-Name": "DA Shutter", "405 AO-IOChannel": "Dev1/ao0", "Core-Camera": "PRIME", "Focus-Name": "Focus", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "405 Shutter-IOChannel": "Dev1/ao8", "640 AO-Demo": "No", "640 Shutter-SupportsTriggering": "0", "445 AO-Demo": "No", "555-DA Device": "555 Shutter", "470 Shutter-Description": "NI DAC", "528 AO-Volts": "0.0000", "COM1-DataBits": "8", "Vincent-D3-Address": "x", "520 AO-SupportsTriggering": "0", "405-Description": "DA device that is used as a shutter", "640 Shutter-MinVolts": "0.0000", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "405 AO-Name": "AnalogIO", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "640 AO-TriggerInputLine": "/Dev1/PFI0", "405 AO-TriggerInputLine": "/Dev1/PFI0", "640-Description": "DA device that is used as a shutter", "445 Shutter-TriggerSampleRate": "100000", "470 Shutter-TriggerSequenceLength": "1000", "COM1-StopBits": "1", "520 Shutter-SupportsTriggering": "0", "405-State": "0", "Core-Initialize": "1", "XLIGHT Emission Wheel-State": "3", "520 AO-Description": "NI DAC", "528 AO-TriggerInputLine": "/Dev1/PFI0", "445 Shutter-MaxVolts": "5.0000", "COM9-Handshaking": "Off", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "XLIGHT Spin motor-Label": "On (spinning)", "PRIME-PP 5 ENABLED": "No", "UUID": "41ba1f6d-2b16-4736-9e21-36e9543a950b", "PRIME-TriggerMode": "Internal Trigger", "COM1-Handshaking": "Off", "PRIME-Timing-PostTriggerDelayNs": "49344000.0000", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "Focus-Position": "17741.5000", "528 Shutter-Block voltage": "false", "Channel": "P-DIC", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "555 Shutter-Demo": "No", "405 Shutter-Demo": "No", "528 AO-Name": "AnalogIO", "520-State": "0", "Core-Galvo": "", "405 Shutter-TriggerSampleRate": "100000", "Focus-FocusStepSize_(nm)": "100", "PRIME-PixelType": "16bit", "COM4-DataBits": "8", "470 AO-IOChannel": "Dev1/ao2", "520 AO-MinVolts": "0.0000", "COM9-Parity": "None", "PRIME-CircularBufferEnabled": "ON", "PRIME-PP 6 Reset": "No", "640 AO-Description": "NI DAC", "Source": "PRIME", "445 AO-MinVolts": "0.0000", "PIEZO-Name": "Stage", "445 AO-Description": "NI DAC", "PRIME-CircularBufferFrameCount": "46", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "520 AO-Volts": "0.0000", "528 Shutter-TriggerSampleRate": "100000", "XPositionUm": 15712.1, "OpticalPath-State": "4", "PRIME-PP 3 THRESHOLD": "80", "PRIME-PVCAM Version": "3.7.1", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Gain": "1-HDR", "640 Shutter-TriggerSequenceLength": "1000", "528 AO-SupportsTriggering": "0", "445 AO-TriggerInputLine": "/Dev1/PFI0", "OpticalPath-Name": "OpticalPath", "PRIME-PP 4 ENABLED": "Yes", "PRIME-PVCAM Adapter Version": "1.3.29", "COM3-Name": "COM3", "405 AO-MaxVolts": "5.0000", "520 Shutter-Block voltage": "false", "PRIME-Timing-ClearingTimeNs": "49344000.0000", "Core-Focus": "PIEZO", "528 AO-Block voltage": "false", "TE2000-Description": "Nikon TE2000 hub", "528 AO-MinVolts": "0.0000", "COM4-Parity": "None", "Summary": { "Slices": 11, "ChContrastMin": [ 0, 0, 0, 0, 0, 0 ], "PixelAspect": 1, "SlicesFirst": true, "Interval_ms": 0, "Time": "2019-12-18 23:10:50 -0500", "Prefix": "seq_5", "InitialPositionList": [ { "Label": "Pos0", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.21], "Focus": [17741.5], "XYStage": [ 44542.200000000004, 31636.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos1", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.22], "Focus": [17741.4], "XYStage": [ 43801.100000000006, 31637.9 ] }, "GridColumnIndex": 0 }, { "Label": "Pos2", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.5], "XYStage": [ 38561.3, 32040.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos3", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.185], "Focus": [17741.3], "XYStage": [ 37698.4, 32041.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos4", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.16], "Focus": [17741.5], "XYStage": [ 32375.4, 31758.9 ] }, "GridColumnIndex": 0 }, { "Label": "Pos5", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.17000000000002], "Focus": [17741.3], "XYStage": [ 31770.7, 31758.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos6", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.4], "XYStage": [ 26536.800000000003, 31068.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos7", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.205], "Focus": [17741.4], "XYStage": [ 25420.2, 31068.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos8", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.85], "Focus": [17741.3], "XYStage": [ 21059, 31508 ] }, "GridColumnIndex": 0 }, { "Label": "Pos9", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.82], "Focus": [17741.3], "XYStage": [ 19731.9, 31506.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos10", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.79], "Focus": [17741.4], "XYStage": [ 14528.5, 32007.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos11", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.83], "Focus": [17741.2], "XYStage": [ 13830.300000000001, 32009.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos12", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.79], "Focus": [17741.3], "XYStage": [ 8775.300000000001, 32017.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos13", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.815], "Focus": [17741.3], "XYStage": [ 8109.200000000001, 32008.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos14", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.805], "Focus": [17741.3], "XYStage": [ 3483.3, 31908.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos15", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.82], "Focus": [17741.2], "XYStage": [ 2686.8, 31903.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos16", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [211.48000000000002], "Focus": [17741.1], "XYStage": [ -1978.9, 31973.600000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos17", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [211.51500000000001], "Focus": [17741.1], "XYStage": [ -2957.8, 31982.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos18", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.195], "Focus": [17741.5], "XYStage": [ 47747.4, -576.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos19", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.23000000000002], "Focus": [17741.6], "XYStage": [ 47053.9, -576.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos20", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.205], "Focus": [17741.4], "XYStage": [ 41871.200000000004, -479.8 ] }, "GridColumnIndex": 0 }, { "Label": "Pos21", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.4], "XYStage": [ 40882.600000000006, -392 ] }, "GridColumnIndex": 0 }, { "Label": "Pos22", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.24], "Focus": [17741.4], "XYStage": [ 35905.6, -135.3 ] }, "GridColumnIndex": 0 }, { "Label": "Pos23", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.195], "Focus": [17741.5], "XYStage": [ 34990.9, -132.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos24", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.46], "Focus": [17741.4], "XYStage": [ 29678.100000000002, -958.1 ] }, "GridColumnIndex": 0 }, { "Label": "Pos25", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.44], "Focus": [17741.5], "XYStage": [ 28983.600000000002, -939.4000000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos26", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.475], "Focus": [17741.5], "XYStage": [ 23019.7, -122 ] }, "GridColumnIndex": 0 }, { "Label": "Pos27", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.44], "Focus": [17741.7], "XYStage": [ 22428, -116.80000000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos28", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.46], "Focus": [17741.7], "XYStage": [ 16364.900000000001, -181.10000000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos29", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.48000000000002], "Focus": [17741.5], "XYStage": [ 15712.1, -180 ] }, "GridColumnIndex": 0 }, { "Label": "Pos30", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.45000000000002], "Focus": [17741.7], "XYStage": [ 10346.1, 77.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos31", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.49], "Focus": [17741.4], "XYStage": [ 9755.5, 85.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos32", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.475], "Focus": [17741.5], "XYStage": [ 4775.5, 293.90000000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos33", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.445], "Focus": [17741.4], "XYStage": [ 3954.9, 293.90000000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos34", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.455], "Focus": [17741.4], "XYStage": [ -1324.9, 545.6 ] }, "GridColumnIndex": 0 }, { "Label": "Pos35", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.445], "Focus": [17741.3], "XYStage": [ -2097, 576.6 ] }, "GridColumnIndex": 0 } ], "z-step_um": 10, "GridRow": 0, "BitDepth": 16, "Depth": 2, "Channels": 6, "Source": "Micro-Manager", "ChColors": [ -16711681, -256, -65281, -1, -1, -16776961 ], "MetadataVersion": 10, "KeepShutterOpenChannels": true, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536, 65536 ], "CustomIntervals_ms": [], "Height": 1200, "MicroManagerVersion": "1.4.23 20180219", "GridColumn": 0, "UUID": "67598d21-5db5-4842-ab60-f506f7b42c7c", "PixelType": "GRAY16", "Width": 1200, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\Yan\\20191218-YC113-S7S8-miseq", "Frames": 1, "Comment": "", "UserName": "Nikon", "PixelSize_um": 1.1, "ROI": [ 0, 0, 1200, 1200 ], "Date": "2019-12-18", "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC", "P-DAPI-high" ], "KeepShutterOpenSlices": true, "IJType": 1, "ComputerName": "ZadorConfocal2", "Positions": 36, "PositionIndex": 29, "CameraTimeout": 20000, "TimeFirst": false }, "640 AO-Name": "AnalogIO", "Vincent-D3-Command": "Close" }, "FrameKey-0-4-4": { "COM4-StopBits": "1", "528 AO-MaxVolts": "5.0000", "XLIGHT Excitation Wheel-State": "1", "470 Shutter-Name": "AnalogIO", "Core-Shutter": "Vincent-D3", "555 AO-Demo": "No", "XLIGHT Dichroic-State": "2", "XYStage-StepSize-Y": "0.1000", "ZPositionUm": 208.48000000000002, "XYStage-StepSize-X": "0.1000", "PRIME-FirmwareVersion": "20.28", "PRIME-ClearCycles": "2", "520-Name": "DA Shutter", "405 Shutter-Name": "AnalogIO", "PIEZO-StepSize": "0.0050", "PRIME-ClearMode": "Pre-Exposure", "470 AO-MinVolts": "0.0000", "405 AO-SupportsTriggering": "1", "CameraChannelIndex": 0, "XYStage-StartSpeed": "500.0000", "COM9-StopBits": "2", "405 AO-Description": "NI DAC", "COM4-DelayBetweenCharsMs": "0.0000", "445 AO-Block voltage": "false", "520 AO-TriggerInputLine": "/Dev1/PFI0", "555 AO-Description": "NI DAC", "520 AO-TriggerSampleRate": "100000", "COM4-Verbose": "1", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM4-Name": "COM4", "405 Shutter-Volts": "0.0000", "Vincent-D3-Port": "COM1", "PRIME-PP 4 MIN ADU AFFECTED": "200", "470 Shutter-MinVolts": "0.0000", "PRIME-PP 3 ENABLED": "Yes", "470 AO-Description": "NI DAC", "PRIME-Description": "PVCAM API device adapter", "520 Shutter-MaxVolts": "5.0000", "520 Shutter-Volts": "0.0000", "528 AO-Sequenceable": "1", "PRIME-Binning": "1x1", "555 Shutter-MaxVolts": "5.0000", "528 Shutter-TriggerSequenceLength": "1000", "405 Shutter-Block voltage": "false", "XLIGHT Spin motor-State": "1", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "Vincent-D3-Time to open (ms)": "35.0000", "Vincent-D3-Time to close (ms)": "35.0000", "445 AO-TriggerSampleRate": "100000", "640 Shutter-IOChannel": "Dev1/ao14", "555 AO-Sequenceable": "1", "520 AO-Name": "AnalogIO", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "470 AO-Block voltage": "false", "COM1-Verbose": "1", "COM9-AnswerTimeout": "10000.0000", "528 Shutter-Name": "AnalogIO", "PRIME-Trigger Timeout (secs)": "10", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "NextFrame": 0, "470 AO-TriggerSequenceLength": "1000", "PRIME-TransposeXY": "0", "445 AO-TriggerSequenceLength": "1000", "COM1-Name": "COM1", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "COM9-DataBits": "8", "COM1-AnswerTimeout": "500.0000", "Camera": "", "TE2000-Version": "V2.50-V2.50-V2.10", "528 Shutter-Volts": "0.0000", "XLIGHT Hub-Name": "XLIGHT Hub", "PIEZO-Description": "Ludl stage driver adapter", "PositionName": "Pos29", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSampleRate": "100000", "PRIME-FanSpeedSetpoint": "High", "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "405 AO-TriggerSampleRate": "100000", "470 Shutter-Block voltage": "false", "470-State": "0", "COM3-Parity": "None", "405 AO-Volts": "0.0000", "PRIME-TransposeMirrorY": "0", "PRIME-ChipName": "GS144BSI", "470 AO-Volts": "0.0000", "PRIME-TransposeMirrorX": "0", "445 Shutter-SupportsTriggering": "0", "470 Shutter-SupportsTriggering": "0", "XLIGHT Hub-Description": "XLIGHT hub", "PRIME-PreampOffLimit": "10000", "470 AO-Sequenceable": "1", "528 Shutter-MaxVolts": "5.0000", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "Core-XYStage": "XYStage", "470 AO-TriggerSampleRate": "100000", "PRIME-Timing-PreTriggerDelayNs": "24672000.0000", "528-DA Device": "528 Shutter", "Focus-Description": "Nikon TE2000 focus adapter", "FilterBlock-State": "5", "520 AO-Demo": "No", "COM3-DelayBetweenCharsMs": "0.0000", "470 Shutter-Volts": "0.0000", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "PRIME-PP 2 THRESHOLD": "125", "PRIME-PP 4 THRESHOLD": "75", "XYStage-Acceleration": "4.0000", "470 Shutter-Demo": "No", "PRIME-Name": "PMPCIECam00", "640-DA Device": "640 Shutter", "405 AO-Block voltage": "false", "ElapsedTime-ms": 1219370, "PRIME-Offset": "100", "445 AO-IOChannel": "Dev1/ao1", "640 AO-MaxVolts": "5.0000", "PositionIndex": 29, "PRIME-PP 1 ENABLED": "Yes", "COM1-BaudRate": "9600", "640-State": "0", "COM3-Verbose": "1", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "LudlController-TransmissionDelay": "0", "PRIME-FullWellCapacity": "62000", "PRIME-SMARTStreamingEnabled": "No", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "640 Shutter-Description": "NI DAC", "528-Description": "DA device that is used as a shutter", "COM4-Description": "Serial port driver (boost:asio)", "405 Shutter-Description": "NI DAC", "TE2000-Name": "TE2000", "528 Shutter-Description": "NI DAC", "COM9-Description": "Serial port driver (boost:asio)", "LudlController-Name": "LudlController", "445 Shutter-TriggerSequenceLength": "1000", "PRIME-SerialNumber": "A18B203008", "445 AO-Volts": "0.0000", "PRIME-X-dimension": "1200", "PRIME-ShutterCloseDelay": "0", "XLIGHT Disk slider-State": "2", "Position": "Default", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 AO-IOChannel": "Dev1/ao5", "520 AO-IOChannel": "Dev1/ao4", "528 AO-TriggerSequenceLength": "1000", "405 Shutter-MaxVolts": "5.0000", "470-DA Device": "470 Shutter", "PRIME-FTCapable": "No", "Core-ChannelGroup": "Channels", "555 AO-SupportsTriggering": "0", "PRIME-Y-dimension": "1200", "Frame": 0, "555 Shutter-Sequenceable": "1", "PRIME-Timing-ExposureTimeNs": "499998000.0000", "Core-ImageProcessor": "", "528-State": "0", "520 AO-Block voltage": "false", "XLIGHT Excitation Wheel-Label": "Excitation-2", "640 Shutter-Demo": "No", "COM3-StopBits": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "520 Shutter-IOChannel": "Dev1/ao11", "Width": 1200, "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-SupportsTriggering": "0", "640 AO-TriggerSampleRate": "100000", "520-Description": "DA device that is used as a shutter", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "405 Shutter-Sequenceable": "1", "405 Shutter-SupportsTriggering": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-TriggerSampleRate": "100000", "640 Shutter-MaxVolts": "5.0000", "555 AO-MaxVolts": "5.0000", "520 AO-TriggerSequenceLength": "1000", "COM3-BaudRate": "9600", "COM9-BaudRate": "9600", "Vincent-D3-Channel #": "Ch. #1", "PRIME-PMode": "Normal", "OpticalPath-Label": "Left(100)", "BitDepth": 16, "555 AO-TriggerSequenceLength": "1000", "445 Shutter-Description": "NI DAC", "405 AO-Sequenceable": "1", "PRIME-AcquisitionMethod": "Callbacks", "555 AO-Name": "AnalogIO", "PRIME-ShutterMode": "Pre-Exposure", "COM3-Handshaking": "Off", "445 AO-SupportsTriggering": "0", "ROI": "0-0-1200-1200", "COM9-Verbose": "1", "PRIME-Color": "OFF", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "PRIME-ShutterOpenDelay": "0", "COM1-Parity": "None", "Slice": 4, "SlicePosition": -10, "COM9-DelayBetweenCharsMs": "0.0000", "555-Name": "DA Shutter", "PIEZO-LudlSingleAxisName": "B", "XLIGHT Emission Wheel-Label": "Empty", "640 Shutter-Name": "AnalogIO", "640 AO-Sequenceable": "1", "520 Shutter-Demo": "No", "528 Shutter-Demo": "No", "470-Name": "DA Shutter", "520-DA Device": "520 Shutter", "XYStage-Description": "Ludl XY stage driver adapter", "445 Shutter-MinVolts": "0.0000", "528 AO-TriggerSampleRate": "100000", "405 AO-MinVolts": "0.0000", "405 Shutter-TriggerSequenceLength": "1000", "Height": 1200, "555 AO-Block voltage": "false", "640 Shutter-Volts": "0.0000", "PixelType": "GRAY16", "445 Shutter-Block voltage": "false", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "520 Shutter-TriggerSequenceLength": "1000", "COM9-Name": "COM9", "Core-SLM": "", "555 AO-Volts": "0.0000", "555 Shutter-Block voltage": "false", "XYStage-Speed": "1800.0000", "520 AO-MaxVolts": "5.0000", "528 Shutter-SupportsTriggering": "0", "COM1-Description": "Serial port driver (boost:asio)", "PRIME-MetadataEnabled": "No", "520 Shutter-MinVolts": "0.0000", "470 Shutter-Sequenceable": "1", "PRIME-PreampDelay": "0", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-MinVolts": "0.0000", "ChannelIndex": 4, "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Core-AutoShutter": "1", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "Exposure-ms": 20, "PRIME-PP 2 ENABLED": "Yes", "640 Shutter-TriggerSampleRate": "100000", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "470 Shutter-IOChannel": "Dev1/ao10", "405 AO-Demo": "No", "PixelSizeUm": 1.1, "470 AO-Demo": "No", "470 Shutter-MaxVolts": "5.0000", "Vincent-D3-Name": "Vincent-D3", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FileName": "img_000000000_P-DIC_004.tif", "LudlController-Reset": "Operate", "PRIME-Exposure": "20.00", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "445-Name": "DA Shutter", "555 Shutter-Name": "AnalogIO", "520 Shutter-TriggerSampleRate": "100000", "445 Shutter-IOChannel": "Dev1/ao9", "PRIME-TransposeCorrection": "0", "XYStage-StepSize": "0.1000", "445-Description": "DA device that is used as a shutter", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 AO-IOChannel": "Dev1/ao7", "520 Shutter-Name": "AnalogIO", "COM3-DataBits": "8", "640 AO-Volts": "0.0000", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "555 Shutter-IOChannel": "Dev1/ao13", "WaitInterval": null, "520 Shutter-Sequenceable": "1", "YPositionUm": -180, "445-State": "0", "445-DA Device": "445 Shutter", "PRIME-CircularBufferAutoSize": "ON", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "555 AO-TriggerSampleRate": "100000", "445 Shutter-Volts": "0.0000", "XLIGHT Hub-Port": "COM3", "Core-AutoFocus": "", "COM4-Handshaking": "Off", "528 Shutter-MinVolts": "0.0000", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "445 Shutter-Name": "AnalogIO", "528 Shutter-Sequenceable": "1", "XYStage-TransposeMirrorX": "0", "XYStage-TransposeMirrorY": "0", "PRIME-ReadoutRate": "100MHz 16bit", "XYStage-Name": "Stage", "640 AO-SupportsTriggering": "0", "640-Name": "DA Shutter", "640 Shutter-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "640 AO-Block voltage": "false", "445 Shutter-Sequenceable": "1", "470 AO-MaxVolts": "5.0000", "COM4-BaudRate": "9600", "470-Description": "DA device that is used as a shutter", "520 Shutter-Description": "NI DAC", "PRIME-CCDTemperature": "-14.8500", "Time": "2019-12-18 23:11:19 -0500", "640 AO-TriggerSequenceLength": "1000", "470 AO-Name": "AnalogIO", "XLIGHT Disk slider-Label": "Disk Pos 40um", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "Binning": "1x1", "TE2000-ModelType": "1", "COM3-AnswerTimeout": "500.0000", "555 Shutter-SupportsTriggering": "0", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "TE2000-Port": "COM4", "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Description": "NI DAC", "COM4-AnswerTimeout": "500.0000", "COM1-DelayBetweenCharsMs": "0.0000", "FilterBlock-Label": "Confocal", "445 AO-Name": "AnalogIO", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-Timing-ReadoutTimeNs": "24672000.0000", "528 AO-Demo": "No", "520 AO-Sequenceable": "1", "555-Description": "DA device that is used as a shutter", "405-DA Device": "405 Shutter", "405 AO-TriggerSequenceLength": "1000", "640 AO-MinVolts": "0.0000", "640 Shutter-Block voltage": "false", "FrameIndex": 0, "PRIME-Port": "CMOS", "555 AO-MinVolts": "0.0000", "COM3-Description": "Serial port driver (boost:asio)", "528 AO-Description": "NI DAC", "555 AO-IOChannel": "Dev1/ao6", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "555 AO-TriggerInputLine": "/Dev1/PFI0", "PRIME-ActualInterval-ms": "390.5200", "LudlController-Port": "COM9", "445 Shutter-Demo": "No", "PRIME-Trigger-Expose Out-Mux": "1", "SliceIndex": 4, "PRIME-CCDTemperatureSetPoint": "-15.0000", "405 Shutter-MinVolts": "0.0000", "PRIME-ExposeOutMode": "First Row", "AxisPositions": null, "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Volts": "0.0000", "528-Name": "DA Shutter", "555-State": "0", "XLIGHT Dichroic-Label": "Di03-R635-t1", "445 AO-Sequenceable": "1", "405-Name": "DA Shutter", "405 AO-IOChannel": "Dev1/ao0", "Core-Camera": "PRIME", "Focus-Name": "Focus", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "405 Shutter-IOChannel": "Dev1/ao8", "640 AO-Demo": "No", "640 Shutter-SupportsTriggering": "0", "445 AO-Demo": "No", "555-DA Device": "555 Shutter", "470 Shutter-Description": "NI DAC", "528 AO-Volts": "0.0000", "COM1-DataBits": "8", "Vincent-D3-Address": "x", "520 AO-SupportsTriggering": "0", "405-Description": "DA device that is used as a shutter", "640 Shutter-MinVolts": "0.0000", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "405 AO-Name": "AnalogIO", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "640 AO-TriggerInputLine": "/Dev1/PFI0", "405 AO-TriggerInputLine": "/Dev1/PFI0", "640-Description": "DA device that is used as a shutter", "445 Shutter-TriggerSampleRate": "100000", "470 Shutter-TriggerSequenceLength": "1000", "COM1-StopBits": "1", "520 Shutter-SupportsTriggering": "0", "405-State": "0", "Core-Initialize": "1", "XLIGHT Emission Wheel-State": "3", "520 AO-Description": "NI DAC", "528 AO-TriggerInputLine": "/Dev1/PFI0", "445 Shutter-MaxVolts": "5.0000", "COM9-Handshaking": "Off", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "XLIGHT Spin motor-Label": "On (spinning)", "PRIME-PP 5 ENABLED": "No", "UUID": "06b67877-30e0-42ee-8ddb-36294daef24f", "PRIME-TriggerMode": "Internal Trigger", "COM1-Handshaking": "Off", "PRIME-Timing-PostTriggerDelayNs": "49344000.0000", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "Focus-Position": "17741.5000", "528 Shutter-Block voltage": "false", "Channel": "P-DIC", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "555 Shutter-Demo": "No", "405 Shutter-Demo": "No", "528 AO-Name": "AnalogIO", "520-State": "0", "Core-Galvo": "", "405 Shutter-TriggerSampleRate": "100000", "Focus-FocusStepSize_(nm)": "100", "PRIME-PixelType": "16bit", "COM4-DataBits": "8", "470 AO-IOChannel": "Dev1/ao2", "520 AO-MinVolts": "0.0000", "COM9-Parity": "None", "PRIME-CircularBufferEnabled": "ON", "PRIME-PP 6 Reset": "No", "640 AO-Description": "NI DAC", "Source": "PRIME", "445 AO-MinVolts": "0.0000", "PIEZO-Name": "Stage", "445 AO-Description": "NI DAC", "PRIME-CircularBufferFrameCount": "46", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "520 AO-Volts": "0.0000", "528 Shutter-TriggerSampleRate": "100000", "XPositionUm": 15712.1, "OpticalPath-State": "4", "PRIME-PP 3 THRESHOLD": "80", "PRIME-PVCAM Version": "3.7.1", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Gain": "1-HDR", "640 Shutter-TriggerSequenceLength": "1000", "528 AO-SupportsTriggering": "0", "445 AO-TriggerInputLine": "/Dev1/PFI0", "OpticalPath-Name": "OpticalPath", "PRIME-PP 4 ENABLED": "Yes", "PRIME-PVCAM Adapter Version": "1.3.29", "COM3-Name": "COM3", "405 AO-MaxVolts": "5.0000", "520 Shutter-Block voltage": "false", "PRIME-Timing-ClearingTimeNs": "49344000.0000", "Core-Focus": "PIEZO", "528 AO-Block voltage": "false", "TE2000-Description": "Nikon TE2000 hub", "528 AO-MinVolts": "0.0000", "COM4-Parity": "None", "Summary": { "Slices": 11, "ChContrastMin": [ 0, 0, 0, 0, 0, 0 ], "PixelAspect": 1, "SlicesFirst": true, "Interval_ms": 0, "Time": "2019-12-18 23:10:50 -0500", "Prefix": "seq_5", "InitialPositionList": [ { "Label": "Pos0", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.21], "Focus": [17741.5], "XYStage": [ 44542.200000000004, 31636.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos1", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.22], "Focus": [17741.4], "XYStage": [ 43801.100000000006, 31637.9 ] }, "GridColumnIndex": 0 }, { "Label": "Pos2", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.5], "XYStage": [ 38561.3, 32040.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos3", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.185], "Focus": [17741.3], "XYStage": [ 37698.4, 32041.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos4", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.16], "Focus": [17741.5], "XYStage": [ 32375.4, 31758.9 ] }, "GridColumnIndex": 0 }, { "Label": "Pos5", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.17000000000002], "Focus": [17741.3], "XYStage": [ 31770.7, 31758.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos6", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.4], "XYStage": [ 26536.800000000003, 31068.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos7", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.205], "Focus": [17741.4], "XYStage": [ 25420.2, 31068.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos8", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.85], "Focus": [17741.3], "XYStage": [ 21059, 31508 ] }, "GridColumnIndex": 0 }, { "Label": "Pos9", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.82], "Focus": [17741.3], "XYStage": [ 19731.9, 31506.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos10", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.79], "Focus": [17741.4], "XYStage": [ 14528.5, 32007.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos11", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.83], "Focus": [17741.2], "XYStage": [ 13830.300000000001, 32009.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos12", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.79], "Focus": [17741.3], "XYStage": [ 8775.300000000001, 32017.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos13", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.815], "Focus": [17741.3], "XYStage": [ 8109.200000000001, 32008.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos14", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.805], "Focus": [17741.3], "XYStage": [ 3483.3, 31908.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos15", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.82], "Focus": [17741.2], "XYStage": [ 2686.8, 31903.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos16", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [211.48000000000002], "Focus": [17741.1], "XYStage": [ -1978.9, 31973.600000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos17", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [211.51500000000001], "Focus": [17741.1], "XYStage": [ -2957.8, 31982.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos18", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.195], "Focus": [17741.5], "XYStage": [ 47747.4, -576.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos19", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.23000000000002], "Focus": [17741.6], "XYStage": [ 47053.9, -576.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos20", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.205], "Focus": [17741.4], "XYStage": [ 41871.200000000004, -479.8 ] }, "GridColumnIndex": 0 }, { "Label": "Pos21", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.4], "XYStage": [ 40882.600000000006, -392 ] }, "GridColumnIndex": 0 }, { "Label": "Pos22", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.24], "Focus": [17741.4], "XYStage": [ 35905.6, -135.3 ] }, "GridColumnIndex": 0 }, { "Label": "Pos23", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.195], "Focus": [17741.5], "XYStage": [ 34990.9, -132.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos24", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.46], "Focus": [17741.4], "XYStage": [ 29678.100000000002, -958.1 ] }, "GridColumnIndex": 0 }, { "Label": "Pos25", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.44], "Focus": [17741.5], "XYStage": [ 28983.600000000002, -939.4000000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos26", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.475], "Focus": [17741.5], "XYStage": [ 23019.7, -122 ] }, "GridColumnIndex": 0 }, { "Label": "Pos27", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.44], "Focus": [17741.7], "XYStage": [ 22428, -116.80000000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos28", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.46], "Focus": [17741.7], "XYStage": [ 16364.900000000001, -181.10000000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos29", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.48000000000002], "Focus": [17741.5], "XYStage": [ 15712.1, -180 ] }, "GridColumnIndex": 0 }, { "Label": "Pos30", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.45000000000002], "Focus": [17741.7], "XYStage": [ 10346.1, 77.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos31", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.49], "Focus": [17741.4], "XYStage": [ 9755.5, 85.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos32", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.475], "Focus": [17741.5], "XYStage": [ 4775.5, 293.90000000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos33", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.445], "Focus": [17741.4], "XYStage": [ 3954.9, 293.90000000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos34", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.455], "Focus": [17741.4], "XYStage": [ -1324.9, 545.6 ] }, "GridColumnIndex": 0 }, { "Label": "Pos35", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.445], "Focus": [17741.3], "XYStage": [ -2097, 576.6 ] }, "GridColumnIndex": 0 } ], "z-step_um": 10, "GridRow": 0, "BitDepth": 16, "Depth": 2, "Channels": 6, "Source": "Micro-Manager", "ChColors": [ -16711681, -256, -65281, -1, -1, -16776961 ], "MetadataVersion": 10, "KeepShutterOpenChannels": true, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536, 65536 ], "CustomIntervals_ms": [], "Height": 1200, "MicroManagerVersion": "1.4.23 20180219", "GridColumn": 0, "UUID": "67598d21-5db5-4842-ab60-f506f7b42c7c", "PixelType": "GRAY16", "Width": 1200, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\Yan\\20191218-YC113-S7S8-miseq", "Frames": 1, "Comment": "", "UserName": "Nikon", "PixelSize_um": 1.1, "ROI": [ 0, 0, 1200, 1200 ], "Date": "2019-12-18", "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC", "P-DAPI-high" ], "KeepShutterOpenSlices": true, "IJType": 1, "ComputerName": "ZadorConfocal2", "Positions": 36, "PositionIndex": 29, "CameraTimeout": 20000, "TimeFirst": false }, "640 AO-Name": "AnalogIO", "Vincent-D3-Command": "Close" }, "FrameKey-0-4-5": { "COM4-StopBits": "1", "528 AO-MaxVolts": "5.0000", "XLIGHT Excitation Wheel-State": "1", "470 Shutter-Name": "AnalogIO", "Core-Shutter": "Vincent-D3", "555 AO-Demo": "No", "XLIGHT Dichroic-State": "2", "XYStage-StepSize-Y": "0.1000", "ZPositionUm": 218.48000000000002, "XYStage-StepSize-X": "0.1000", "PRIME-FirmwareVersion": "20.28", "PRIME-ClearCycles": "2", "520-Name": "DA Shutter", "405 Shutter-Name": "AnalogIO", "PIEZO-StepSize": "0.0050", "PRIME-ClearMode": "Pre-Exposure", "470 AO-MinVolts": "0.0000", "405 AO-SupportsTriggering": "1", "CameraChannelIndex": 0, "XYStage-StartSpeed": "500.0000", "COM9-StopBits": "2", "405 AO-Description": "NI DAC", "COM4-DelayBetweenCharsMs": "0.0000", "445 AO-Block voltage": "false", "520 AO-TriggerInputLine": "/Dev1/PFI0", "555 AO-Description": "NI DAC", "520 AO-TriggerSampleRate": "100000", "COM4-Verbose": "1", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM4-Name": "COM4", "405 Shutter-Volts": "0.0000", "Vincent-D3-Port": "COM1", "PRIME-PP 4 MIN ADU AFFECTED": "200", "470 Shutter-MinVolts": "0.0000", "PRIME-PP 3 ENABLED": "Yes", "470 AO-Description": "NI DAC", "PRIME-Description": "PVCAM API device adapter", "520 Shutter-MaxVolts": "5.0000", "520 Shutter-Volts": "0.0000", "528 AO-Sequenceable": "1", "PRIME-Binning": "1x1", "555 Shutter-MaxVolts": "5.0000", "528 Shutter-TriggerSequenceLength": "1000", "405 Shutter-Block voltage": "false", "XLIGHT Spin motor-State": "1", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "Vincent-D3-Time to open (ms)": "35.0000", "Vincent-D3-Time to close (ms)": "35.0000", "445 AO-TriggerSampleRate": "100000", "640 Shutter-IOChannel": "Dev1/ao14", "555 AO-Sequenceable": "1", "520 AO-Name": "AnalogIO", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "470 AO-Block voltage": "false", "COM1-Verbose": "1", "COM9-AnswerTimeout": "10000.0000", "528 Shutter-Name": "AnalogIO", "PRIME-Trigger Timeout (secs)": "10", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "NextFrame": 0, "470 AO-TriggerSequenceLength": "1000", "PRIME-TransposeXY": "0", "445 AO-TriggerSequenceLength": "1000", "COM1-Name": "COM1", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "COM9-DataBits": "8", "COM1-AnswerTimeout": "500.0000", "Camera": "", "TE2000-Version": "V2.50-V2.50-V2.10", "528 Shutter-Volts": "0.0000", "XLIGHT Hub-Name": "XLIGHT Hub", "PIEZO-Description": "Ludl stage driver adapter", "PositionName": "Pos29", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSampleRate": "100000", "PRIME-FanSpeedSetpoint": "High", "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "405 AO-TriggerSampleRate": "100000", "470 Shutter-Block voltage": "false", "470-State": "0", "COM3-Parity": "None", "405 AO-Volts": "0.0000", "PRIME-TransposeMirrorY": "0", "PRIME-ChipName": "GS144BSI", "470 AO-Volts": "0.0000", "PRIME-TransposeMirrorX": "0", "445 Shutter-SupportsTriggering": "0", "470 Shutter-SupportsTriggering": "0", "XLIGHT Hub-Description": "XLIGHT hub", "PRIME-PreampOffLimit": "10000", "470 AO-Sequenceable": "1", "528 Shutter-MaxVolts": "5.0000", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "Core-XYStage": "XYStage", "470 AO-TriggerSampleRate": "100000", "PRIME-Timing-PreTriggerDelayNs": "24672000.0000", "528-DA Device": "528 Shutter", "Focus-Description": "Nikon TE2000 focus adapter", "FilterBlock-State": "5", "520 AO-Demo": "No", "COM3-DelayBetweenCharsMs": "0.0000", "470 Shutter-Volts": "0.0000", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "PRIME-PP 2 THRESHOLD": "125", "PRIME-PP 4 THRESHOLD": "75", "XYStage-Acceleration": "4.0000", "470 Shutter-Demo": "No", "PRIME-Name": "PMPCIECam00", "640-DA Device": "640 Shutter", "405 AO-Block voltage": "false", "ElapsedTime-ms": 1219637, "PRIME-Offset": "100", "445 AO-IOChannel": "Dev1/ao1", "640 AO-MaxVolts": "5.0000", "PositionIndex": 29, "PRIME-PP 1 ENABLED": "Yes", "COM1-BaudRate": "9600", "640-State": "0", "COM3-Verbose": "1", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "LudlController-TransmissionDelay": "0", "PRIME-FullWellCapacity": "62000", "PRIME-SMARTStreamingEnabled": "No", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "640 Shutter-Description": "NI DAC", "528-Description": "DA device that is used as a shutter", "COM4-Description": "Serial port driver (boost:asio)", "405 Shutter-Description": "NI DAC", "TE2000-Name": "TE2000", "528 Shutter-Description": "NI DAC", "COM9-Description": "Serial port driver (boost:asio)", "LudlController-Name": "LudlController", "445 Shutter-TriggerSequenceLength": "1000", "PRIME-SerialNumber": "A18B203008", "445 AO-Volts": "0.0000", "PRIME-X-dimension": "1200", "PRIME-ShutterCloseDelay": "0", "XLIGHT Disk slider-State": "2", "Position": "Default", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 AO-IOChannel": "Dev1/ao5", "520 AO-IOChannel": "Dev1/ao4", "528 AO-TriggerSequenceLength": "1000", "405 Shutter-MaxVolts": "5.0000", "470-DA Device": "470 Shutter", "PRIME-FTCapable": "No", "Core-ChannelGroup": "Channels", "555 AO-SupportsTriggering": "0", "PRIME-Y-dimension": "1200", "Frame": 0, "555 Shutter-Sequenceable": "1", "PRIME-Timing-ExposureTimeNs": "499998000.0000", "Core-ImageProcessor": "", "528-State": "0", "520 AO-Block voltage": "false", "XLIGHT Excitation Wheel-Label": "Excitation-2", "640 Shutter-Demo": "No", "COM3-StopBits": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "520 Shutter-IOChannel": "Dev1/ao11", "Width": 1200, "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-SupportsTriggering": "0", "640 AO-TriggerSampleRate": "100000", "520-Description": "DA device that is used as a shutter", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "405 Shutter-Sequenceable": "1", "405 Shutter-SupportsTriggering": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-TriggerSampleRate": "100000", "640 Shutter-MaxVolts": "5.0000", "555 AO-MaxVolts": "5.0000", "520 AO-TriggerSequenceLength": "1000", "COM3-BaudRate": "9600", "COM9-BaudRate": "9600", "Vincent-D3-Channel #": "Ch. #1", "PRIME-PMode": "Normal", "OpticalPath-Label": "Left(100)", "BitDepth": 16, "555 AO-TriggerSequenceLength": "1000", "445 Shutter-Description": "NI DAC", "405 AO-Sequenceable": "1", "PRIME-AcquisitionMethod": "Callbacks", "555 AO-Name": "AnalogIO", "PRIME-ShutterMode": "Pre-Exposure", "COM3-Handshaking": "Off", "445 AO-SupportsTriggering": "0", "ROI": "0-0-1200-1200", "COM9-Verbose": "1", "PRIME-Color": "OFF", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "PRIME-ShutterOpenDelay": "0", "COM1-Parity": "None", "Slice": 5, "SlicePosition": 0, "COM9-DelayBetweenCharsMs": "0.0000", "555-Name": "DA Shutter", "PIEZO-LudlSingleAxisName": "B", "XLIGHT Emission Wheel-Label": "Empty", "640 Shutter-Name": "AnalogIO", "640 AO-Sequenceable": "1", "520 Shutter-Demo": "No", "528 Shutter-Demo": "No", "470-Name": "DA Shutter", "520-DA Device": "520 Shutter", "XYStage-Description": "Ludl XY stage driver adapter", "445 Shutter-MinVolts": "0.0000", "528 AO-TriggerSampleRate": "100000", "405 AO-MinVolts": "0.0000", "405 Shutter-TriggerSequenceLength": "1000", "Height": 1200, "555 AO-Block voltage": "false", "640 Shutter-Volts": "0.0000", "PixelType": "GRAY16", "445 Shutter-Block voltage": "false", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "520 Shutter-TriggerSequenceLength": "1000", "COM9-Name": "COM9", "Core-SLM": "", "555 AO-Volts": "0.0000", "555 Shutter-Block voltage": "false", "XYStage-Speed": "1800.0000", "520 AO-MaxVolts": "5.0000", "528 Shutter-SupportsTriggering": "0", "COM1-Description": "Serial port driver (boost:asio)", "PRIME-MetadataEnabled": "No", "520 Shutter-MinVolts": "0.0000", "470 Shutter-Sequenceable": "1", "PRIME-PreampDelay": "0", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-MinVolts": "0.0000", "ChannelIndex": 4, "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Core-AutoShutter": "1", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "Exposure-ms": 20, "PRIME-PP 2 ENABLED": "Yes", "640 Shutter-TriggerSampleRate": "100000", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "470 Shutter-IOChannel": "Dev1/ao10", "405 AO-Demo": "No", "PixelSizeUm": 1.1, "470 AO-Demo": "No", "470 Shutter-MaxVolts": "5.0000", "Vincent-D3-Name": "Vincent-D3", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FileName": "img_000000000_P-DIC_005.tif", "LudlController-Reset": "Operate", "PRIME-Exposure": "20.00", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "445-Name": "DA Shutter", "555 Shutter-Name": "AnalogIO", "520 Shutter-TriggerSampleRate": "100000", "445 Shutter-IOChannel": "Dev1/ao9", "PRIME-TransposeCorrection": "0", "XYStage-StepSize": "0.1000", "445-Description": "DA device that is used as a shutter", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 AO-IOChannel": "Dev1/ao7", "520 Shutter-Name": "AnalogIO", "COM3-DataBits": "8", "640 AO-Volts": "0.0000", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "555 Shutter-IOChannel": "Dev1/ao13", "WaitInterval": null, "520 Shutter-Sequenceable": "1", "YPositionUm": -180, "445-State": "0", "445-DA Device": "445 Shutter", "PRIME-CircularBufferAutoSize": "ON", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "555 AO-TriggerSampleRate": "100000", "445 Shutter-Volts": "0.0000", "XLIGHT Hub-Port": "COM3", "Core-AutoFocus": "", "COM4-Handshaking": "Off", "528 Shutter-MinVolts": "0.0000", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "445 Shutter-Name": "AnalogIO", "528 Shutter-Sequenceable": "1", "XYStage-TransposeMirrorX": "0", "XYStage-TransposeMirrorY": "0", "PRIME-ReadoutRate": "100MHz 16bit", "XYStage-Name": "Stage", "640 AO-SupportsTriggering": "0", "640-Name": "DA Shutter", "640 Shutter-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "640 AO-Block voltage": "false", "445 Shutter-Sequenceable": "1", "470 AO-MaxVolts": "5.0000", "COM4-BaudRate": "9600", "470-Description": "DA device that is used as a shutter", "520 Shutter-Description": "NI DAC", "PRIME-CCDTemperature": "-14.8500", "Time": "2019-12-18 23:11:19 -0500", "640 AO-TriggerSequenceLength": "1000", "470 AO-Name": "AnalogIO", "XLIGHT Disk slider-Label": "Disk Pos 40um", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "Binning": "1x1", "TE2000-ModelType": "1", "COM3-AnswerTimeout": "500.0000", "555 Shutter-SupportsTriggering": "0", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "TE2000-Port": "COM4", "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Description": "NI DAC", "COM4-AnswerTimeout": "500.0000", "COM1-DelayBetweenCharsMs": "0.0000", "FilterBlock-Label": "Confocal", "445 AO-Name": "AnalogIO", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-Timing-ReadoutTimeNs": "24672000.0000", "528 AO-Demo": "No", "520 AO-Sequenceable": "1", "555-Description": "DA device that is used as a shutter", "405-DA Device": "405 Shutter", "405 AO-TriggerSequenceLength": "1000", "640 AO-MinVolts": "0.0000", "640 Shutter-Block voltage": "false", "FrameIndex": 0, "PRIME-Port": "CMOS", "555 AO-MinVolts": "0.0000", "COM3-Description": "Serial port driver (boost:asio)", "528 AO-Description": "NI DAC", "555 AO-IOChannel": "Dev1/ao6", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "555 AO-TriggerInputLine": "/Dev1/PFI0", "PRIME-ActualInterval-ms": "390.5200", "LudlController-Port": "COM9", "445 Shutter-Demo": "No", "PRIME-Trigger-Expose Out-Mux": "1", "SliceIndex": 5, "PRIME-CCDTemperatureSetPoint": "-15.0000", "405 Shutter-MinVolts": "0.0000", "PRIME-ExposeOutMode": "First Row", "AxisPositions": null, "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Volts": "0.0000", "528-Name": "DA Shutter", "555-State": "0", "XLIGHT Dichroic-Label": "Di03-R635-t1", "445 AO-Sequenceable": "1", "405-Name": "DA Shutter", "405 AO-IOChannel": "Dev1/ao0", "Core-Camera": "PRIME", "Focus-Name": "Focus", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "405 Shutter-IOChannel": "Dev1/ao8", "640 AO-Demo": "No", "640 Shutter-SupportsTriggering": "0", "445 AO-Demo": "No", "555-DA Device": "555 Shutter", "470 Shutter-Description": "NI DAC", "528 AO-Volts": "0.0000", "COM1-DataBits": "8", "Vincent-D3-Address": "x", "520 AO-SupportsTriggering": "0", "405-Description": "DA device that is used as a shutter", "640 Shutter-MinVolts": "0.0000", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "405 AO-Name": "AnalogIO", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "640 AO-TriggerInputLine": "/Dev1/PFI0", "405 AO-TriggerInputLine": "/Dev1/PFI0", "640-Description": "DA device that is used as a shutter", "445 Shutter-TriggerSampleRate": "100000", "470 Shutter-TriggerSequenceLength": "1000", "COM1-StopBits": "1", "520 Shutter-SupportsTriggering": "0", "405-State": "0", "Core-Initialize": "1", "XLIGHT Emission Wheel-State": "3", "520 AO-Description": "NI DAC", "528 AO-TriggerInputLine": "/Dev1/PFI0", "445 Shutter-MaxVolts": "5.0000", "COM9-Handshaking": "Off", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "XLIGHT Spin motor-Label": "On (spinning)", "PRIME-PP 5 ENABLED": "No", "UUID": "fffac46d-4c25-4e53-988f-883acab1364f", "PRIME-TriggerMode": "Internal Trigger", "COM1-Handshaking": "Off", "PRIME-Timing-PostTriggerDelayNs": "49344000.0000", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "Focus-Position": "17741.5000", "528 Shutter-Block voltage": "false", "Channel": "P-DIC", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "555 Shutter-Demo": "No", "405 Shutter-Demo": "No", "528 AO-Name": "AnalogIO", "520-State": "0", "Core-Galvo": "", "405 Shutter-TriggerSampleRate": "100000", "Focus-FocusStepSize_(nm)": "100", "PRIME-PixelType": "16bit", "COM4-DataBits": "8", "470 AO-IOChannel": "Dev1/ao2", "520 AO-MinVolts": "0.0000", "COM9-Parity": "None", "PRIME-CircularBufferEnabled": "ON", "PRIME-PP 6 Reset": "No", "640 AO-Description": "NI DAC", "Source": "PRIME", "445 AO-MinVolts": "0.0000", "PIEZO-Name": "Stage", "445 AO-Description": "NI DAC", "PRIME-CircularBufferFrameCount": "46", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "520 AO-Volts": "0.0000", "528 Shutter-TriggerSampleRate": "100000", "XPositionUm": 15712.1, "OpticalPath-State": "4", "PRIME-PP 3 THRESHOLD": "80", "PRIME-PVCAM Version": "3.7.1", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Gain": "1-HDR", "640 Shutter-TriggerSequenceLength": "1000", "528 AO-SupportsTriggering": "0", "445 AO-TriggerInputLine": "/Dev1/PFI0", "OpticalPath-Name": "OpticalPath", "PRIME-PP 4 ENABLED": "Yes", "PRIME-PVCAM Adapter Version": "1.3.29", "COM3-Name": "COM3", "405 AO-MaxVolts": "5.0000", "520 Shutter-Block voltage": "false", "PRIME-Timing-ClearingTimeNs": "49344000.0000", "Core-Focus": "PIEZO", "528 AO-Block voltage": "false", "TE2000-Description": "Nikon TE2000 hub", "528 AO-MinVolts": "0.0000", "COM4-Parity": "None", "Summary": { "Slices": 11, "ChContrastMin": [ 0, 0, 0, 0, 0, 0 ], "PixelAspect": 1, "SlicesFirst": true, "Interval_ms": 0, "Time": "2019-12-18 23:10:50 -0500", "Prefix": "seq_5", "InitialPositionList": [ { "Label": "Pos0", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.21], "Focus": [17741.5], "XYStage": [ 44542.200000000004, 31636.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos1", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.22], "Focus": [17741.4], "XYStage": [ 43801.100000000006, 31637.9 ] }, "GridColumnIndex": 0 }, { "Label": "Pos2", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.5], "XYStage": [ 38561.3, 32040.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos3", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.185], "Focus": [17741.3], "XYStage": [ 37698.4, 32041.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos4", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.16], "Focus": [17741.5], "XYStage": [ 32375.4, 31758.9 ] }, "GridColumnIndex": 0 }, { "Label": "Pos5", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.17000000000002], "Focus": [17741.3], "XYStage": [ 31770.7, 31758.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos6", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.4], "XYStage": [ 26536.800000000003, 31068.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos7", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.205], "Focus": [17741.4], "XYStage": [ 25420.2, 31068.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos8", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.85], "Focus": [17741.3], "XYStage": [ 21059, 31508 ] }, "GridColumnIndex": 0 }, { "Label": "Pos9", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.82], "Focus": [17741.3], "XYStage": [ 19731.9, 31506.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos10", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.79], "Focus": [17741.4], "XYStage": [ 14528.5, 32007.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos11", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.83], "Focus": [17741.2], "XYStage": [ 13830.300000000001, 32009.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos12", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.79], "Focus": [17741.3], "XYStage": [ 8775.300000000001, 32017.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos13", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.815], "Focus": [17741.3], "XYStage": [ 8109.200000000001, 32008.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos14", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.805], "Focus": [17741.3], "XYStage": [ 3483.3, 31908.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos15", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.82], "Focus": [17741.2], "XYStage": [ 2686.8, 31903.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos16", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [211.48000000000002], "Focus": [17741.1], "XYStage": [ -1978.9, 31973.600000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos17", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [211.51500000000001], "Focus": [17741.1], "XYStage": [ -2957.8, 31982.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos18", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.195], "Focus": [17741.5], "XYStage": [ 47747.4, -576.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos19", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.23000000000002], "Focus": [17741.6], "XYStage": [ 47053.9, -576.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos20", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.205], "Focus": [17741.4], "XYStage": [ 41871.200000000004, -479.8 ] }, "GridColumnIndex": 0 }, { "Label": "Pos21", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.4], "XYStage": [ 40882.600000000006, -392 ] }, "GridColumnIndex": 0 }, { "Label": "Pos22", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.24], "Focus": [17741.4], "XYStage": [ 35905.6, -135.3 ] }, "GridColumnIndex": 0 }, { "Label": "Pos23", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.195], "Focus": [17741.5], "XYStage": [ 34990.9, -132.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos24", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.46], "Focus": [17741.4], "XYStage": [ 29678.100000000002, -958.1 ] }, "GridColumnIndex": 0 }, { "Label": "Pos25", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.44], "Focus": [17741.5], "XYStage": [ 28983.600000000002, -939.4000000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos26", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.475], "Focus": [17741.5], "XYStage": [ 23019.7, -122 ] }, "GridColumnIndex": 0 }, { "Label": "Pos27", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.44], "Focus": [17741.7], "XYStage": [ 22428, -116.80000000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos28", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.46], "Focus": [17741.7], "XYStage": [ 16364.900000000001, -181.10000000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos29", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.48000000000002], "Focus": [17741.5], "XYStage": [ 15712.1, -180 ] }, "GridColumnIndex": 0 }, { "Label": "Pos30", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.45000000000002], "Focus": [17741.7], "XYStage": [ 10346.1, 77.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos31", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.49], "Focus": [17741.4], "XYStage": [ 9755.5, 85.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos32", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.475], "Focus": [17741.5], "XYStage": [ 4775.5, 293.90000000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos33", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.445], "Focus": [17741.4], "XYStage": [ 3954.9, 293.90000000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos34", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.455], "Focus": [17741.4], "XYStage": [ -1324.9, 545.6 ] }, "GridColumnIndex": 0 }, { "Label": "Pos35", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.445], "Focus": [17741.3], "XYStage": [ -2097, 576.6 ] }, "GridColumnIndex": 0 } ], "z-step_um": 10, "GridRow": 0, "BitDepth": 16, "Depth": 2, "Channels": 6, "Source": "Micro-Manager", "ChColors": [ -16711681, -256, -65281, -1, -1, -16776961 ], "MetadataVersion": 10, "KeepShutterOpenChannels": true, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536, 65536 ], "CustomIntervals_ms": [], "Height": 1200, "MicroManagerVersion": "1.4.23 20180219", "GridColumn": 0, "UUID": "67598d21-5db5-4842-ab60-f506f7b42c7c", "PixelType": "GRAY16", "Width": 1200, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\Yan\\20191218-YC113-S7S8-miseq", "Frames": 1, "Comment": "", "UserName": "Nikon", "PixelSize_um": 1.1, "ROI": [ 0, 0, 1200, 1200 ], "Date": "2019-12-18", "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC", "P-DAPI-high" ], "KeepShutterOpenSlices": true, "IJType": 1, "ComputerName": "ZadorConfocal2", "Positions": 36, "PositionIndex": 29, "CameraTimeout": 20000, "TimeFirst": false }, "640 AO-Name": "AnalogIO", "Vincent-D3-Command": "Close" }, "FrameKey-0-4-6": { "COM4-StopBits": "1", "528 AO-MaxVolts": "5.0000", "XLIGHT Excitation Wheel-State": "1", "470 Shutter-Name": "AnalogIO", "Core-Shutter": "Vincent-D3", "555 AO-Demo": "No", "XLIGHT Dichroic-State": "2", "XYStage-StepSize-Y": "0.1000", "ZPositionUm": 228.48000000000002, "XYStage-StepSize-X": "0.1000", "PRIME-FirmwareVersion": "20.28", "PRIME-ClearCycles": "2", "520-Name": "DA Shutter", "405 Shutter-Name": "AnalogIO", "PIEZO-StepSize": "0.0050", "PRIME-ClearMode": "Pre-Exposure", "470 AO-MinVolts": "0.0000", "405 AO-SupportsTriggering": "1", "CameraChannelIndex": 0, "XYStage-StartSpeed": "500.0000", "COM9-StopBits": "2", "405 AO-Description": "NI DAC", "COM4-DelayBetweenCharsMs": "0.0000", "445 AO-Block voltage": "false", "520 AO-TriggerInputLine": "/Dev1/PFI0", "555 AO-Description": "NI DAC", "520 AO-TriggerSampleRate": "100000", "COM4-Verbose": "1", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM4-Name": "COM4", "405 Shutter-Volts": "0.0000", "Vincent-D3-Port": "COM1", "PRIME-PP 4 MIN ADU AFFECTED": "200", "470 Shutter-MinVolts": "0.0000", "PRIME-PP 3 ENABLED": "Yes", "470 AO-Description": "NI DAC", "PRIME-Description": "PVCAM API device adapter", "520 Shutter-MaxVolts": "5.0000", "520 Shutter-Volts": "0.0000", "528 AO-Sequenceable": "1", "PRIME-Binning": "1x1", "555 Shutter-MaxVolts": "5.0000", "528 Shutter-TriggerSequenceLength": "1000", "405 Shutter-Block voltage": "false", "XLIGHT Spin motor-State": "1", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "Vincent-D3-Time to open (ms)": "35.0000", "Vincent-D3-Time to close (ms)": "35.0000", "445 AO-TriggerSampleRate": "100000", "640 Shutter-IOChannel": "Dev1/ao14", "555 AO-Sequenceable": "1", "520 AO-Name": "AnalogIO", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "470 AO-Block voltage": "false", "COM1-Verbose": "1", "COM9-AnswerTimeout": "10000.0000", "528 Shutter-Name": "AnalogIO", "PRIME-Trigger Timeout (secs)": "10", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "NextFrame": 0, "470 AO-TriggerSequenceLength": "1000", "PRIME-TransposeXY": "0", "445 AO-TriggerSequenceLength": "1000", "COM1-Name": "COM1", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "COM9-DataBits": "8", "COM1-AnswerTimeout": "500.0000", "Camera": "", "TE2000-Version": "V2.50-V2.50-V2.10", "528 Shutter-Volts": "0.0000", "XLIGHT Hub-Name": "XLIGHT Hub", "PIEZO-Description": "Ludl stage driver adapter", "PositionName": "Pos29", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSampleRate": "100000", "PRIME-FanSpeedSetpoint": "High", "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "405 AO-TriggerSampleRate": "100000", "470 Shutter-Block voltage": "false", "470-State": "0", "COM3-Parity": "None", "405 AO-Volts": "0.0000", "PRIME-TransposeMirrorY": "0", "PRIME-ChipName": "GS144BSI", "470 AO-Volts": "0.0000", "PRIME-TransposeMirrorX": "0", "445 Shutter-SupportsTriggering": "0", "470 Shutter-SupportsTriggering": "0", "XLIGHT Hub-Description": "XLIGHT hub", "PRIME-PreampOffLimit": "10000", "470 AO-Sequenceable": "1", "528 Shutter-MaxVolts": "5.0000", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "Core-XYStage": "XYStage", "470 AO-TriggerSampleRate": "100000", "PRIME-Timing-PreTriggerDelayNs": "24672000.0000", "528-DA Device": "528 Shutter", "Focus-Description": "Nikon TE2000 focus adapter", "FilterBlock-State": "5", "520 AO-Demo": "No", "COM3-DelayBetweenCharsMs": "0.0000", "470 Shutter-Volts": "0.0000", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "PRIME-PP 2 THRESHOLD": "125", "PRIME-PP 4 THRESHOLD": "75", "XYStage-Acceleration": "4.0000", "470 Shutter-Demo": "No", "PRIME-Name": "PMPCIECam00", "640-DA Device": "640 Shutter", "405 AO-Block voltage": "false", "ElapsedTime-ms": 1219908, "PRIME-Offset": "100", "445 AO-IOChannel": "Dev1/ao1", "640 AO-MaxVolts": "5.0000", "PositionIndex": 29, "PRIME-PP 1 ENABLED": "Yes", "COM1-BaudRate": "9600", "640-State": "0", "COM3-Verbose": "1", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "LudlController-TransmissionDelay": "0", "PRIME-FullWellCapacity": "62000", "PRIME-SMARTStreamingEnabled": "No", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "640 Shutter-Description": "NI DAC", "528-Description": "DA device that is used as a shutter", "COM4-Description": "Serial port driver (boost:asio)", "405 Shutter-Description": "NI DAC", "TE2000-Name": "TE2000", "528 Shutter-Description": "NI DAC", "COM9-Description": "Serial port driver (boost:asio)", "LudlController-Name": "LudlController", "445 Shutter-TriggerSequenceLength": "1000", "PRIME-SerialNumber": "A18B203008", "445 AO-Volts": "0.0000", "PRIME-X-dimension": "1200", "PRIME-ShutterCloseDelay": "0", "XLIGHT Disk slider-State": "2", "Position": "Default", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 AO-IOChannel": "Dev1/ao5", "520 AO-IOChannel": "Dev1/ao4", "528 AO-TriggerSequenceLength": "1000", "405 Shutter-MaxVolts": "5.0000", "470-DA Device": "470 Shutter", "PRIME-FTCapable": "No", "Core-ChannelGroup": "Channels", "555 AO-SupportsTriggering": "0", "PRIME-Y-dimension": "1200", "Frame": 0, "555 Shutter-Sequenceable": "1", "PRIME-Timing-ExposureTimeNs": "499998000.0000", "Core-ImageProcessor": "", "528-State": "0", "520 AO-Block voltage": "false", "XLIGHT Excitation Wheel-Label": "Excitation-2", "640 Shutter-Demo": "No", "COM3-StopBits": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "520 Shutter-IOChannel": "Dev1/ao11", "Width": 1200, "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-SupportsTriggering": "0", "640 AO-TriggerSampleRate": "100000", "520-Description": "DA device that is used as a shutter", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "405 Shutter-Sequenceable": "1", "405 Shutter-SupportsTriggering": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-TriggerSampleRate": "100000", "640 Shutter-MaxVolts": "5.0000", "555 AO-MaxVolts": "5.0000", "520 AO-TriggerSequenceLength": "1000", "COM3-BaudRate": "9600", "COM9-BaudRate": "9600", "Vincent-D3-Channel #": "Ch. #1", "PRIME-PMode": "Normal", "OpticalPath-Label": "Left(100)", "BitDepth": 16, "555 AO-TriggerSequenceLength": "1000", "445 Shutter-Description": "NI DAC", "405 AO-Sequenceable": "1", "PRIME-AcquisitionMethod": "Callbacks", "555 AO-Name": "AnalogIO", "PRIME-ShutterMode": "Pre-Exposure", "COM3-Handshaking": "Off", "445 AO-SupportsTriggering": "0", "ROI": "0-0-1200-1200", "COM9-Verbose": "1", "PRIME-Color": "OFF", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "PRIME-ShutterOpenDelay": "0", "COM1-Parity": "None", "Slice": 6, "SlicePosition": 10, "COM9-DelayBetweenCharsMs": "0.0000", "555-Name": "DA Shutter", "PIEZO-LudlSingleAxisName": "B", "XLIGHT Emission Wheel-Label": "Empty", "640 Shutter-Name": "AnalogIO", "640 AO-Sequenceable": "1", "520 Shutter-Demo": "No", "528 Shutter-Demo": "No", "470-Name": "DA Shutter", "520-DA Device": "520 Shutter", "XYStage-Description": "Ludl XY stage driver adapter", "445 Shutter-MinVolts": "0.0000", "528 AO-TriggerSampleRate": "100000", "405 AO-MinVolts": "0.0000", "405 Shutter-TriggerSequenceLength": "1000", "Height": 1200, "555 AO-Block voltage": "false", "640 Shutter-Volts": "0.0000", "PixelType": "GRAY16", "445 Shutter-Block voltage": "false", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "520 Shutter-TriggerSequenceLength": "1000", "COM9-Name": "COM9", "Core-SLM": "", "555 AO-Volts": "0.0000", "555 Shutter-Block voltage": "false", "XYStage-Speed": "1800.0000", "520 AO-MaxVolts": "5.0000", "528 Shutter-SupportsTriggering": "0", "COM1-Description": "Serial port driver (boost:asio)", "PRIME-MetadataEnabled": "No", "520 Shutter-MinVolts": "0.0000", "470 Shutter-Sequenceable": "1", "PRIME-PreampDelay": "0", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-MinVolts": "0.0000", "ChannelIndex": 4, "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Core-AutoShutter": "1", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "Exposure-ms": 20, "PRIME-PP 2 ENABLED": "Yes", "640 Shutter-TriggerSampleRate": "100000", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "470 Shutter-IOChannel": "Dev1/ao10", "405 AO-Demo": "No", "PixelSizeUm": 1.1, "470 AO-Demo": "No", "470 Shutter-MaxVolts": "5.0000", "Vincent-D3-Name": "Vincent-D3", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FileName": "img_000000000_P-DIC_006.tif", "LudlController-Reset": "Operate", "PRIME-Exposure": "20.00", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "445-Name": "DA Shutter", "555 Shutter-Name": "AnalogIO", "520 Shutter-TriggerSampleRate": "100000", "445 Shutter-IOChannel": "Dev1/ao9", "PRIME-TransposeCorrection": "0", "XYStage-StepSize": "0.1000", "445-Description": "DA device that is used as a shutter", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 AO-IOChannel": "Dev1/ao7", "520 Shutter-Name": "AnalogIO", "COM3-DataBits": "8", "640 AO-Volts": "0.0000", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "555 Shutter-IOChannel": "Dev1/ao13", "WaitInterval": null, "520 Shutter-Sequenceable": "1", "YPositionUm": -180, "445-State": "0", "445-DA Device": "445 Shutter", "PRIME-CircularBufferAutoSize": "ON", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "555 AO-TriggerSampleRate": "100000", "445 Shutter-Volts": "0.0000", "XLIGHT Hub-Port": "COM3", "Core-AutoFocus": "", "COM4-Handshaking": "Off", "528 Shutter-MinVolts": "0.0000", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "445 Shutter-Name": "AnalogIO", "528 Shutter-Sequenceable": "1", "XYStage-TransposeMirrorX": "0", "XYStage-TransposeMirrorY": "0", "PRIME-ReadoutRate": "100MHz 16bit", "XYStage-Name": "Stage", "640 AO-SupportsTriggering": "0", "640-Name": "DA Shutter", "640 Shutter-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "640 AO-Block voltage": "false", "445 Shutter-Sequenceable": "1", "470 AO-MaxVolts": "5.0000", "COM4-BaudRate": "9600", "470-Description": "DA device that is used as a shutter", "520 Shutter-Description": "NI DAC", "PRIME-CCDTemperature": "-14.8500", "Time": "2019-12-18 23:11:20 -0500", "640 AO-TriggerSequenceLength": "1000", "470 AO-Name": "AnalogIO", "XLIGHT Disk slider-Label": "Disk Pos 40um", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "Binning": "1x1", "TE2000-ModelType": "1", "COM3-AnswerTimeout": "500.0000", "555 Shutter-SupportsTriggering": "0", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "TE2000-Port": "COM4", "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Description": "NI DAC", "COM4-AnswerTimeout": "500.0000", "COM1-DelayBetweenCharsMs": "0.0000", "FilterBlock-Label": "Confocal", "445 AO-Name": "AnalogIO", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-Timing-ReadoutTimeNs": "24672000.0000", "528 AO-Demo": "No", "520 AO-Sequenceable": "1", "555-Description": "DA device that is used as a shutter", "405-DA Device": "405 Shutter", "405 AO-TriggerSequenceLength": "1000", "640 AO-MinVolts": "0.0000", "640 Shutter-Block voltage": "false", "FrameIndex": 0, "PRIME-Port": "CMOS", "555 AO-MinVolts": "0.0000", "COM3-Description": "Serial port driver (boost:asio)", "528 AO-Description": "NI DAC", "555 AO-IOChannel": "Dev1/ao6", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "555 AO-TriggerInputLine": "/Dev1/PFI0", "PRIME-ActualInterval-ms": "390.5200", "LudlController-Port": "COM9", "445 Shutter-Demo": "No", "PRIME-Trigger-Expose Out-Mux": "1", "SliceIndex": 6, "PRIME-CCDTemperatureSetPoint": "-15.0000", "405 Shutter-MinVolts": "0.0000", "PRIME-ExposeOutMode": "First Row", "AxisPositions": null, "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Volts": "0.0000", "528-Name": "DA Shutter", "555-State": "0", "XLIGHT Dichroic-Label": "Di03-R635-t1", "445 AO-Sequenceable": "1", "405-Name": "DA Shutter", "405 AO-IOChannel": "Dev1/ao0", "Core-Camera": "PRIME", "Focus-Name": "Focus", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "405 Shutter-IOChannel": "Dev1/ao8", "640 AO-Demo": "No", "640 Shutter-SupportsTriggering": "0", "445 AO-Demo": "No", "555-DA Device": "555 Shutter", "470 Shutter-Description": "NI DAC", "528 AO-Volts": "0.0000", "COM1-DataBits": "8", "Vincent-D3-Address": "x", "520 AO-SupportsTriggering": "0", "405-Description": "DA device that is used as a shutter", "640 Shutter-MinVolts": "0.0000", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "405 AO-Name": "AnalogIO", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "640 AO-TriggerInputLine": "/Dev1/PFI0", "405 AO-TriggerInputLine": "/Dev1/PFI0", "640-Description": "DA device that is used as a shutter", "445 Shutter-TriggerSampleRate": "100000", "470 Shutter-TriggerSequenceLength": "1000", "COM1-StopBits": "1", "520 Shutter-SupportsTriggering": "0", "405-State": "0", "Core-Initialize": "1", "XLIGHT Emission Wheel-State": "3", "520 AO-Description": "NI DAC", "528 AO-TriggerInputLine": "/Dev1/PFI0", "445 Shutter-MaxVolts": "5.0000", "COM9-Handshaking": "Off", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "XLIGHT Spin motor-Label": "On (spinning)", "PRIME-PP 5 ENABLED": "No", "UUID": "0335a4f8-45a2-4c16-9bed-e65589f2bf6e", "PRIME-TriggerMode": "Internal Trigger", "COM1-Handshaking": "Off", "PRIME-Timing-PostTriggerDelayNs": "49344000.0000", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "Focus-Position": "17741.5000", "528 Shutter-Block voltage": "false", "Channel": "P-DIC", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "555 Shutter-Demo": "No", "405 Shutter-Demo": "No", "528 AO-Name": "AnalogIO", "520-State": "0", "Core-Galvo": "", "405 Shutter-TriggerSampleRate": "100000", "Focus-FocusStepSize_(nm)": "100", "PRIME-PixelType": "16bit", "COM4-DataBits": "8", "470 AO-IOChannel": "Dev1/ao2", "520 AO-MinVolts": "0.0000", "COM9-Parity": "None", "PRIME-CircularBufferEnabled": "ON", "PRIME-PP 6 Reset": "No", "640 AO-Description": "NI DAC", "Source": "PRIME", "445 AO-MinVolts": "0.0000", "PIEZO-Name": "Stage", "445 AO-Description": "NI DAC", "PRIME-CircularBufferFrameCount": "46", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "520 AO-Volts": "0.0000", "528 Shutter-TriggerSampleRate": "100000", "XPositionUm": 15712.1, "OpticalPath-State": "4", "PRIME-PP 3 THRESHOLD": "80", "PRIME-PVCAM Version": "3.7.1", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Gain": "1-HDR", "640 Shutter-TriggerSequenceLength": "1000", "528 AO-SupportsTriggering": "0", "445 AO-TriggerInputLine": "/Dev1/PFI0", "OpticalPath-Name": "OpticalPath", "PRIME-PP 4 ENABLED": "Yes", "PRIME-PVCAM Adapter Version": "1.3.29", "COM3-Name": "COM3", "405 AO-MaxVolts": "5.0000", "520 Shutter-Block voltage": "false", "PRIME-Timing-ClearingTimeNs": "49344000.0000", "Core-Focus": "PIEZO", "528 AO-Block voltage": "false", "TE2000-Description": "Nikon TE2000 hub", "528 AO-MinVolts": "0.0000", "COM4-Parity": "None", "Summary": { "Slices": 11, "ChContrastMin": [ 0, 0, 0, 0, 0, 0 ], "PixelAspect": 1, "SlicesFirst": true, "Interval_ms": 0, "Time": "2019-12-18 23:10:50 -0500", "Prefix": "seq_5", "InitialPositionList": [ { "Label": "Pos0", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.21], "Focus": [17741.5], "XYStage": [ 44542.200000000004, 31636.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos1", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.22], "Focus": [17741.4], "XYStage": [ 43801.100000000006, 31637.9 ] }, "GridColumnIndex": 0 }, { "Label": "Pos2", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.5], "XYStage": [ 38561.3, 32040.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos3", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.185], "Focus": [17741.3], "XYStage": [ 37698.4, 32041.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos4", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.16], "Focus": [17741.5], "XYStage": [ 32375.4, 31758.9 ] }, "GridColumnIndex": 0 }, { "Label": "Pos5", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.17000000000002], "Focus": [17741.3], "XYStage": [ 31770.7, 31758.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos6", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.4], "XYStage": [ 26536.800000000003, 31068.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos7", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.205], "Focus": [17741.4], "XYStage": [ 25420.2, 31068.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos8", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.85], "Focus": [17741.3], "XYStage": [ 21059, 31508 ] }, "GridColumnIndex": 0 }, { "Label": "Pos9", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.82], "Focus": [17741.3], "XYStage": [ 19731.9, 31506.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos10", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.79], "Focus": [17741.4], "XYStage": [ 14528.5, 32007.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos11", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.83], "Focus": [17741.2], "XYStage": [ 13830.300000000001, 32009.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos12", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.79], "Focus": [17741.3], "XYStage": [ 8775.300000000001, 32017.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos13", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.815], "Focus": [17741.3], "XYStage": [ 8109.200000000001, 32008.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos14", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.805], "Focus": [17741.3], "XYStage": [ 3483.3, 31908.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos15", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.82], "Focus": [17741.2], "XYStage": [ 2686.8, 31903.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos16", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [211.48000000000002], "Focus": [17741.1], "XYStage": [ -1978.9, 31973.600000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos17", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [211.51500000000001], "Focus": [17741.1], "XYStage": [ -2957.8, 31982.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos18", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.195], "Focus": [17741.5], "XYStage": [ 47747.4, -576.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos19", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.23000000000002], "Focus": [17741.6], "XYStage": [ 47053.9, -576.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos20", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.205], "Focus": [17741.4], "XYStage": [ 41871.200000000004, -479.8 ] }, "GridColumnIndex": 0 }, { "Label": "Pos21", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.4], "XYStage": [ 40882.600000000006, -392 ] }, "GridColumnIndex": 0 }, { "Label": "Pos22", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.24], "Focus": [17741.4], "XYStage": [ 35905.6, -135.3 ] }, "GridColumnIndex": 0 }, { "Label": "Pos23", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.195], "Focus": [17741.5], "XYStage": [ 34990.9, -132.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos24", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.46], "Focus": [17741.4], "XYStage": [ 29678.100000000002, -958.1 ] }, "GridColumnIndex": 0 }, { "Label": "Pos25", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.44], "Focus": [17741.5], "XYStage": [ 28983.600000000002, -939.4000000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos26", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.475], "Focus": [17741.5], "XYStage": [ 23019.7, -122 ] }, "GridColumnIndex": 0 }, { "Label": "Pos27", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.44], "Focus": [17741.7], "XYStage": [ 22428, -116.80000000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos28", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.46], "Focus": [17741.7], "XYStage": [ 16364.900000000001, -181.10000000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos29", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.48000000000002], "Focus": [17741.5], "XYStage": [ 15712.1, -180 ] }, "GridColumnIndex": 0 }, { "Label": "Pos30", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.45000000000002], "Focus": [17741.7], "XYStage": [ 10346.1, 77.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos31", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.49], "Focus": [17741.4], "XYStage": [ 9755.5, 85.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos32", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.475], "Focus": [17741.5], "XYStage": [ 4775.5, 293.90000000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos33", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.445], "Focus": [17741.4], "XYStage": [ 3954.9, 293.90000000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos34", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.455], "Focus": [17741.4], "XYStage": [ -1324.9, 545.6 ] }, "GridColumnIndex": 0 }, { "Label": "Pos35", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.445], "Focus": [17741.3], "XYStage": [ -2097, 576.6 ] }, "GridColumnIndex": 0 } ], "z-step_um": 10, "GridRow": 0, "BitDepth": 16, "Depth": 2, "Channels": 6, "Source": "Micro-Manager", "ChColors": [ -16711681, -256, -65281, -1, -1, -16776961 ], "MetadataVersion": 10, "KeepShutterOpenChannels": true, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536, 65536 ], "CustomIntervals_ms": [], "Height": 1200, "MicroManagerVersion": "1.4.23 20180219", "GridColumn": 0, "UUID": "67598d21-5db5-4842-ab60-f506f7b42c7c", "PixelType": "GRAY16", "Width": 1200, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\Yan\\20191218-YC113-S7S8-miseq", "Frames": 1, "Comment": "", "UserName": "Nikon", "PixelSize_um": 1.1, "ROI": [ 0, 0, 1200, 1200 ], "Date": "2019-12-18", "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC", "P-DAPI-high" ], "KeepShutterOpenSlices": true, "IJType": 1, "ComputerName": "ZadorConfocal2", "Positions": 36, "PositionIndex": 29, "CameraTimeout": 20000, "TimeFirst": false }, "640 AO-Name": "AnalogIO", "Vincent-D3-Command": "Close" }, "FrameKey-0-4-7": { "COM4-StopBits": "1", "528 AO-MaxVolts": "5.0000", "XLIGHT Excitation Wheel-State": "1", "470 Shutter-Name": "AnalogIO", "Core-Shutter": "Vincent-D3", "555 AO-Demo": "No", "XLIGHT Dichroic-State": "2", "XYStage-StepSize-Y": "0.1000", "ZPositionUm": 238.48000000000002, "XYStage-StepSize-X": "0.1000", "PRIME-FirmwareVersion": "20.28", "PRIME-ClearCycles": "2", "520-Name": "DA Shutter", "405 Shutter-Name": "AnalogIO", "PIEZO-StepSize": "0.0050", "PRIME-ClearMode": "Pre-Exposure", "470 AO-MinVolts": "0.0000", "405 AO-SupportsTriggering": "1", "CameraChannelIndex": 0, "XYStage-StartSpeed": "500.0000", "COM9-StopBits": "2", "405 AO-Description": "NI DAC", "COM4-DelayBetweenCharsMs": "0.0000", "445 AO-Block voltage": "false", "520 AO-TriggerInputLine": "/Dev1/PFI0", "555 AO-Description": "NI DAC", "520 AO-TriggerSampleRate": "100000", "COM4-Verbose": "1", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM4-Name": "COM4", "405 Shutter-Volts": "0.0000", "Vincent-D3-Port": "COM1", "PRIME-PP 4 MIN ADU AFFECTED": "200", "470 Shutter-MinVolts": "0.0000", "PRIME-PP 3 ENABLED": "Yes", "470 AO-Description": "NI DAC", "PRIME-Description": "PVCAM API device adapter", "520 Shutter-MaxVolts": "5.0000", "520 Shutter-Volts": "0.0000", "528 AO-Sequenceable": "1", "PRIME-Binning": "1x1", "555 Shutter-MaxVolts": "5.0000", "528 Shutter-TriggerSequenceLength": "1000", "405 Shutter-Block voltage": "false", "XLIGHT Spin motor-State": "1", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "Vincent-D3-Time to open (ms)": "35.0000", "Vincent-D3-Time to close (ms)": "35.0000", "445 AO-TriggerSampleRate": "100000", "640 Shutter-IOChannel": "Dev1/ao14", "555 AO-Sequenceable": "1", "520 AO-Name": "AnalogIO", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "470 AO-Block voltage": "false", "COM1-Verbose": "1", "COM9-AnswerTimeout": "10000.0000", "528 Shutter-Name": "AnalogIO", "PRIME-Trigger Timeout (secs)": "10", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "NextFrame": 0, "470 AO-TriggerSequenceLength": "1000", "PRIME-TransposeXY": "0", "445 AO-TriggerSequenceLength": "1000", "COM1-Name": "COM1", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "COM9-DataBits": "8", "COM1-AnswerTimeout": "500.0000", "Camera": "", "TE2000-Version": "V2.50-V2.50-V2.10", "528 Shutter-Volts": "0.0000", "XLIGHT Hub-Name": "XLIGHT Hub", "PIEZO-Description": "Ludl stage driver adapter", "PositionName": "Pos29", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSampleRate": "100000", "PRIME-FanSpeedSetpoint": "High", "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "405 AO-TriggerSampleRate": "100000", "470 Shutter-Block voltage": "false", "470-State": "0", "COM3-Parity": "None", "405 AO-Volts": "0.0000", "PRIME-TransposeMirrorY": "0", "PRIME-ChipName": "GS144BSI", "470 AO-Volts": "0.0000", "PRIME-TransposeMirrorX": "0", "445 Shutter-SupportsTriggering": "0", "470 Shutter-SupportsTriggering": "0", "XLIGHT Hub-Description": "XLIGHT hub", "PRIME-PreampOffLimit": "10000", "470 AO-Sequenceable": "1", "528 Shutter-MaxVolts": "5.0000", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "Core-XYStage": "XYStage", "470 AO-TriggerSampleRate": "100000", "PRIME-Timing-PreTriggerDelayNs": "24672000.0000", "528-DA Device": "528 Shutter", "Focus-Description": "Nikon TE2000 focus adapter", "FilterBlock-State": "5", "520 AO-Demo": "No", "COM3-DelayBetweenCharsMs": "0.0000", "470 Shutter-Volts": "0.0000", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "PRIME-PP 2 THRESHOLD": "125", "PRIME-PP 4 THRESHOLD": "75", "XYStage-Acceleration": "4.0000", "470 Shutter-Demo": "No", "PRIME-Name": "PMPCIECam00", "640-DA Device": "640 Shutter", "405 AO-Block voltage": "false", "ElapsedTime-ms": 1220169, "PRIME-Offset": "100", "445 AO-IOChannel": "Dev1/ao1", "640 AO-MaxVolts": "5.0000", "PositionIndex": 29, "PRIME-PP 1 ENABLED": "Yes", "COM1-BaudRate": "9600", "640-State": "0", "COM3-Verbose": "1", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "LudlController-TransmissionDelay": "0", "PRIME-FullWellCapacity": "62000", "PRIME-SMARTStreamingEnabled": "No", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "640 Shutter-Description": "NI DAC", "528-Description": "DA device that is used as a shutter", "COM4-Description": "Serial port driver (boost:asio)", "405 Shutter-Description": "NI DAC", "TE2000-Name": "TE2000", "528 Shutter-Description": "NI DAC", "COM9-Description": "Serial port driver (boost:asio)", "LudlController-Name": "LudlController", "445 Shutter-TriggerSequenceLength": "1000", "PRIME-SerialNumber": "A18B203008", "445 AO-Volts": "0.0000", "PRIME-X-dimension": "1200", "PRIME-ShutterCloseDelay": "0", "XLIGHT Disk slider-State": "2", "Position": "Default", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 AO-IOChannel": "Dev1/ao5", "520 AO-IOChannel": "Dev1/ao4", "528 AO-TriggerSequenceLength": "1000", "405 Shutter-MaxVolts": "5.0000", "470-DA Device": "470 Shutter", "PRIME-FTCapable": "No", "Core-ChannelGroup": "Channels", "555 AO-SupportsTriggering": "0", "PRIME-Y-dimension": "1200", "Frame": 0, "555 Shutter-Sequenceable": "1", "PRIME-Timing-ExposureTimeNs": "499998000.0000", "Core-ImageProcessor": "", "528-State": "0", "520 AO-Block voltage": "false", "XLIGHT Excitation Wheel-Label": "Excitation-2", "640 Shutter-Demo": "No", "COM3-StopBits": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "520 Shutter-IOChannel": "Dev1/ao11", "Width": 1200, "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-SupportsTriggering": "0", "640 AO-TriggerSampleRate": "100000", "520-Description": "DA device that is used as a shutter", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "405 Shutter-Sequenceable": "1", "405 Shutter-SupportsTriggering": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-TriggerSampleRate": "100000", "640 Shutter-MaxVolts": "5.0000", "555 AO-MaxVolts": "5.0000", "520 AO-TriggerSequenceLength": "1000", "COM3-BaudRate": "9600", "COM9-BaudRate": "9600", "Vincent-D3-Channel #": "Ch. #1", "PRIME-PMode": "Normal", "OpticalPath-Label": "Left(100)", "BitDepth": 16, "555 AO-TriggerSequenceLength": "1000", "445 Shutter-Description": "NI DAC", "405 AO-Sequenceable": "1", "PRIME-AcquisitionMethod": "Callbacks", "555 AO-Name": "AnalogIO", "PRIME-ShutterMode": "Pre-Exposure", "COM3-Handshaking": "Off", "445 AO-SupportsTriggering": "0", "ROI": "0-0-1200-1200", "COM9-Verbose": "1", "PRIME-Color": "OFF", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "PRIME-ShutterOpenDelay": "0", "COM1-Parity": "None", "Slice": 7, "SlicePosition": 20, "COM9-DelayBetweenCharsMs": "0.0000", "555-Name": "DA Shutter", "PIEZO-LudlSingleAxisName": "B", "XLIGHT Emission Wheel-Label": "Empty", "640 Shutter-Name": "AnalogIO", "640 AO-Sequenceable": "1", "520 Shutter-Demo": "No", "528 Shutter-Demo": "No", "470-Name": "DA Shutter", "520-DA Device": "520 Shutter", "XYStage-Description": "Ludl XY stage driver adapter", "445 Shutter-MinVolts": "0.0000", "528 AO-TriggerSampleRate": "100000", "405 AO-MinVolts": "0.0000", "405 Shutter-TriggerSequenceLength": "1000", "Height": 1200, "555 AO-Block voltage": "false", "640 Shutter-Volts": "0.0000", "PixelType": "GRAY16", "445 Shutter-Block voltage": "false", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "520 Shutter-TriggerSequenceLength": "1000", "COM9-Name": "COM9", "Core-SLM": "", "555 AO-Volts": "0.0000", "555 Shutter-Block voltage": "false", "XYStage-Speed": "1800.0000", "520 AO-MaxVolts": "5.0000", "528 Shutter-SupportsTriggering": "0", "COM1-Description": "Serial port driver (boost:asio)", "PRIME-MetadataEnabled": "No", "520 Shutter-MinVolts": "0.0000", "470 Shutter-Sequenceable": "1", "PRIME-PreampDelay": "0", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-MinVolts": "0.0000", "ChannelIndex": 4, "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Core-AutoShutter": "1", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "Exposure-ms": 20, "PRIME-PP 2 ENABLED": "Yes", "640 Shutter-TriggerSampleRate": "100000", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "470 Shutter-IOChannel": "Dev1/ao10", "405 AO-Demo": "No", "PixelSizeUm": 1.1, "470 AO-Demo": "No", "470 Shutter-MaxVolts": "5.0000", "Vincent-D3-Name": "Vincent-D3", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FileName": "img_000000000_P-DIC_007.tif", "LudlController-Reset": "Operate", "PRIME-Exposure": "20.00", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "445-Name": "DA Shutter", "555 Shutter-Name": "AnalogIO", "520 Shutter-TriggerSampleRate": "100000", "445 Shutter-IOChannel": "Dev1/ao9", "PRIME-TransposeCorrection": "0", "XYStage-StepSize": "0.1000", "445-Description": "DA device that is used as a shutter", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 AO-IOChannel": "Dev1/ao7", "520 Shutter-Name": "AnalogIO", "COM3-DataBits": "8", "640 AO-Volts": "0.0000", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "555 Shutter-IOChannel": "Dev1/ao13", "WaitInterval": null, "520 Shutter-Sequenceable": "1", "YPositionUm": -180, "445-State": "0", "445-DA Device": "445 Shutter", "PRIME-CircularBufferAutoSize": "ON", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "555 AO-TriggerSampleRate": "100000", "445 Shutter-Volts": "0.0000", "XLIGHT Hub-Port": "COM3", "Core-AutoFocus": "", "COM4-Handshaking": "Off", "528 Shutter-MinVolts": "0.0000", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "445 Shutter-Name": "AnalogIO", "528 Shutter-Sequenceable": "1", "XYStage-TransposeMirrorX": "0", "XYStage-TransposeMirrorY": "0", "PRIME-ReadoutRate": "100MHz 16bit", "XYStage-Name": "Stage", "640 AO-SupportsTriggering": "0", "640-Name": "DA Shutter", "640 Shutter-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "640 AO-Block voltage": "false", "445 Shutter-Sequenceable": "1", "470 AO-MaxVolts": "5.0000", "COM4-BaudRate": "9600", "470-Description": "DA device that is used as a shutter", "520 Shutter-Description": "NI DAC", "PRIME-CCDTemperature": "-14.8500", "Time": "2019-12-18 23:11:20 -0500", "640 AO-TriggerSequenceLength": "1000", "470 AO-Name": "AnalogIO", "XLIGHT Disk slider-Label": "Disk Pos 40um", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "Binning": "1x1", "TE2000-ModelType": "1", "COM3-AnswerTimeout": "500.0000", "555 Shutter-SupportsTriggering": "0", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "TE2000-Port": "COM4", "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Description": "NI DAC", "COM4-AnswerTimeout": "500.0000", "COM1-DelayBetweenCharsMs": "0.0000", "FilterBlock-Label": "Confocal", "445 AO-Name": "AnalogIO", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-Timing-ReadoutTimeNs": "24672000.0000", "528 AO-Demo": "No", "520 AO-Sequenceable": "1", "555-Description": "DA device that is used as a shutter", "405-DA Device": "405 Shutter", "405 AO-TriggerSequenceLength": "1000", "640 AO-MinVolts": "0.0000", "640 Shutter-Block voltage": "false", "FrameIndex": 0, "PRIME-Port": "CMOS", "555 AO-MinVolts": "0.0000", "COM3-Description": "Serial port driver (boost:asio)", "528 AO-Description": "NI DAC", "555 AO-IOChannel": "Dev1/ao6", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "555 AO-TriggerInputLine": "/Dev1/PFI0", "PRIME-ActualInterval-ms": "390.5200", "LudlController-Port": "COM9", "445 Shutter-Demo": "No", "PRIME-Trigger-Expose Out-Mux": "1", "SliceIndex": 7, "PRIME-CCDTemperatureSetPoint": "-15.0000", "405 Shutter-MinVolts": "0.0000", "PRIME-ExposeOutMode": "First Row", "AxisPositions": null, "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Volts": "0.0000", "528-Name": "DA Shutter", "555-State": "0", "XLIGHT Dichroic-Label": "Di03-R635-t1", "445 AO-Sequenceable": "1", "405-Name": "DA Shutter", "405 AO-IOChannel": "Dev1/ao0", "Core-Camera": "PRIME", "Focus-Name": "Focus", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "405 Shutter-IOChannel": "Dev1/ao8", "640 AO-Demo": "No", "640 Shutter-SupportsTriggering": "0", "445 AO-Demo": "No", "555-DA Device": "555 Shutter", "470 Shutter-Description": "NI DAC", "528 AO-Volts": "0.0000", "COM1-DataBits": "8", "Vincent-D3-Address": "x", "520 AO-SupportsTriggering": "0", "405-Description": "DA device that is used as a shutter", "640 Shutter-MinVolts": "0.0000", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "405 AO-Name": "AnalogIO", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "640 AO-TriggerInputLine": "/Dev1/PFI0", "405 AO-TriggerInputLine": "/Dev1/PFI0", "640-Description": "DA device that is used as a shutter", "445 Shutter-TriggerSampleRate": "100000", "470 Shutter-TriggerSequenceLength": "1000", "COM1-StopBits": "1", "520 Shutter-SupportsTriggering": "0", "405-State": "0", "Core-Initialize": "1", "XLIGHT Emission Wheel-State": "3", "520 AO-Description": "NI DAC", "528 AO-TriggerInputLine": "/Dev1/PFI0", "445 Shutter-MaxVolts": "5.0000", "COM9-Handshaking": "Off", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "XLIGHT Spin motor-Label": "On (spinning)", "PRIME-PP 5 ENABLED": "No", "UUID": "fd32ef09-295f-4335-9c97-4672df4918df", "PRIME-TriggerMode": "Internal Trigger", "COM1-Handshaking": "Off", "PRIME-Timing-PostTriggerDelayNs": "49344000.0000", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "Focus-Position": "17741.5000", "528 Shutter-Block voltage": "false", "Channel": "P-DIC", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "555 Shutter-Demo": "No", "405 Shutter-Demo": "No", "528 AO-Name": "AnalogIO", "520-State": "0", "Core-Galvo": "", "405 Shutter-TriggerSampleRate": "100000", "Focus-FocusStepSize_(nm)": "100", "PRIME-PixelType": "16bit", "COM4-DataBits": "8", "470 AO-IOChannel": "Dev1/ao2", "520 AO-MinVolts": "0.0000", "COM9-Parity": "None", "PRIME-CircularBufferEnabled": "ON", "PRIME-PP 6 Reset": "No", "640 AO-Description": "NI DAC", "Source": "PRIME", "445 AO-MinVolts": "0.0000", "PIEZO-Name": "Stage", "445 AO-Description": "NI DAC", "PRIME-CircularBufferFrameCount": "46", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "520 AO-Volts": "0.0000", "528 Shutter-TriggerSampleRate": "100000", "XPositionUm": 15712.1, "OpticalPath-State": "4", "PRIME-PP 3 THRESHOLD": "80", "PRIME-PVCAM Version": "3.7.1", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Gain": "1-HDR", "640 Shutter-TriggerSequenceLength": "1000", "528 AO-SupportsTriggering": "0", "445 AO-TriggerInputLine": "/Dev1/PFI0", "OpticalPath-Name": "OpticalPath", "PRIME-PP 4 ENABLED": "Yes", "PRIME-PVCAM Adapter Version": "1.3.29", "COM3-Name": "COM3", "405 AO-MaxVolts": "5.0000", "520 Shutter-Block voltage": "false", "PRIME-Timing-ClearingTimeNs": "49344000.0000", "Core-Focus": "PIEZO", "528 AO-Block voltage": "false", "TE2000-Description": "Nikon TE2000 hub", "528 AO-MinVolts": "0.0000", "COM4-Parity": "None", "Summary": { "Slices": 11, "ChContrastMin": [ 0, 0, 0, 0, 0, 0 ], "PixelAspect": 1, "SlicesFirst": true, "Interval_ms": 0, "Time": "2019-12-18 23:10:50 -0500", "Prefix": "seq_5", "InitialPositionList": [ { "Label": "Pos0", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.21], "Focus": [17741.5], "XYStage": [ 44542.200000000004, 31636.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos1", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.22], "Focus": [17741.4], "XYStage": [ 43801.100000000006, 31637.9 ] }, "GridColumnIndex": 0 }, { "Label": "Pos2", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.5], "XYStage": [ 38561.3, 32040.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos3", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.185], "Focus": [17741.3], "XYStage": [ 37698.4, 32041.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos4", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.16], "Focus": [17741.5], "XYStage": [ 32375.4, 31758.9 ] }, "GridColumnIndex": 0 }, { "Label": "Pos5", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.17000000000002], "Focus": [17741.3], "XYStage": [ 31770.7, 31758.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos6", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.4], "XYStage": [ 26536.800000000003, 31068.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos7", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.205], "Focus": [17741.4], "XYStage": [ 25420.2, 31068.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos8", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.85], "Focus": [17741.3], "XYStage": [ 21059, 31508 ] }, "GridColumnIndex": 0 }, { "Label": "Pos9", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.82], "Focus": [17741.3], "XYStage": [ 19731.9, 31506.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos10", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.79], "Focus": [17741.4], "XYStage": [ 14528.5, 32007.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos11", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.83], "Focus": [17741.2], "XYStage": [ 13830.300000000001, 32009.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos12", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.79], "Focus": [17741.3], "XYStage": [ 8775.300000000001, 32017.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos13", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.815], "Focus": [17741.3], "XYStage": [ 8109.200000000001, 32008.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos14", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.805], "Focus": [17741.3], "XYStage": [ 3483.3, 31908.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos15", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.82], "Focus": [17741.2], "XYStage": [ 2686.8, 31903.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos16", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [211.48000000000002], "Focus": [17741.1], "XYStage": [ -1978.9, 31973.600000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos17", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [211.51500000000001], "Focus": [17741.1], "XYStage": [ -2957.8, 31982.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos18", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.195], "Focus": [17741.5], "XYStage": [ 47747.4, -576.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos19", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.23000000000002], "Focus": [17741.6], "XYStage": [ 47053.9, -576.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos20", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.205], "Focus": [17741.4], "XYStage": [ 41871.200000000004, -479.8 ] }, "GridColumnIndex": 0 }, { "Label": "Pos21", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.4], "XYStage": [ 40882.600000000006, -392 ] }, "GridColumnIndex": 0 }, { "Label": "Pos22", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.24], "Focus": [17741.4], "XYStage": [ 35905.6, -135.3 ] }, "GridColumnIndex": 0 }, { "Label": "Pos23", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.195], "Focus": [17741.5], "XYStage": [ 34990.9, -132.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos24", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.46], "Focus": [17741.4], "XYStage": [ 29678.100000000002, -958.1 ] }, "GridColumnIndex": 0 }, { "Label": "Pos25", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.44], "Focus": [17741.5], "XYStage": [ 28983.600000000002, -939.4000000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos26", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.475], "Focus": [17741.5], "XYStage": [ 23019.7, -122 ] }, "GridColumnIndex": 0 }, { "Label": "Pos27", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.44], "Focus": [17741.7], "XYStage": [ 22428, -116.80000000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos28", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.46], "Focus": [17741.7], "XYStage": [ 16364.900000000001, -181.10000000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos29", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.48000000000002], "Focus": [17741.5], "XYStage": [ 15712.1, -180 ] }, "GridColumnIndex": 0 }, { "Label": "Pos30", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.45000000000002], "Focus": [17741.7], "XYStage": [ 10346.1, 77.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos31", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.49], "Focus": [17741.4], "XYStage": [ 9755.5, 85.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos32", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.475], "Focus": [17741.5], "XYStage": [ 4775.5, 293.90000000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos33", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.445], "Focus": [17741.4], "XYStage": [ 3954.9, 293.90000000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos34", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.455], "Focus": [17741.4], "XYStage": [ -1324.9, 545.6 ] }, "GridColumnIndex": 0 }, { "Label": "Pos35", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.445], "Focus": [17741.3], "XYStage": [ -2097, 576.6 ] }, "GridColumnIndex": 0 } ], "z-step_um": 10, "GridRow": 0, "BitDepth": 16, "Depth": 2, "Channels": 6, "Source": "Micro-Manager", "ChColors": [ -16711681, -256, -65281, -1, -1, -16776961 ], "MetadataVersion": 10, "KeepShutterOpenChannels": true, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536, 65536 ], "CustomIntervals_ms": [], "Height": 1200, "MicroManagerVersion": "1.4.23 20180219", "GridColumn": 0, "UUID": "67598d21-5db5-4842-ab60-f506f7b42c7c", "PixelType": "GRAY16", "Width": 1200, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\Yan\\20191218-YC113-S7S8-miseq", "Frames": 1, "Comment": "", "UserName": "Nikon", "PixelSize_um": 1.1, "ROI": [ 0, 0, 1200, 1200 ], "Date": "2019-12-18", "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC", "P-DAPI-high" ], "KeepShutterOpenSlices": true, "IJType": 1, "ComputerName": "ZadorConfocal2", "Positions": 36, "PositionIndex": 29, "CameraTimeout": 20000, "TimeFirst": false }, "640 AO-Name": "AnalogIO", "Vincent-D3-Command": "Close" }, "FrameKey-0-4-8": { "COM4-StopBits": "1", "528 AO-MaxVolts": "5.0000", "XLIGHT Excitation Wheel-State": "1", "470 Shutter-Name": "AnalogIO", "Core-Shutter": "Vincent-D3", "555 AO-Demo": "No", "XLIGHT Dichroic-State": "2", "XYStage-StepSize-Y": "0.1000", "ZPositionUm": 248.48000000000002, "XYStage-StepSize-X": "0.1000", "PRIME-FirmwareVersion": "20.28", "PRIME-ClearCycles": "2", "520-Name": "DA Shutter", "405 Shutter-Name": "AnalogIO", "PIEZO-StepSize": "0.0050", "PRIME-ClearMode": "Pre-Exposure", "470 AO-MinVolts": "0.0000", "405 AO-SupportsTriggering": "1", "CameraChannelIndex": 0, "XYStage-StartSpeed": "500.0000", "COM9-StopBits": "2", "405 AO-Description": "NI DAC", "COM4-DelayBetweenCharsMs": "0.0000", "445 AO-Block voltage": "false", "520 AO-TriggerInputLine": "/Dev1/PFI0", "555 AO-Description": "NI DAC", "520 AO-TriggerSampleRate": "100000", "COM4-Verbose": "1", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM4-Name": "COM4", "405 Shutter-Volts": "0.0000", "Vincent-D3-Port": "COM1", "PRIME-PP 4 MIN ADU AFFECTED": "200", "470 Shutter-MinVolts": "0.0000", "PRIME-PP 3 ENABLED": "Yes", "470 AO-Description": "NI DAC", "PRIME-Description": "PVCAM API device adapter", "520 Shutter-MaxVolts": "5.0000", "520 Shutter-Volts": "0.0000", "528 AO-Sequenceable": "1", "PRIME-Binning": "1x1", "555 Shutter-MaxVolts": "5.0000", "528 Shutter-TriggerSequenceLength": "1000", "405 Shutter-Block voltage": "false", "XLIGHT Spin motor-State": "1", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "Vincent-D3-Time to open (ms)": "35.0000", "Vincent-D3-Time to close (ms)": "35.0000", "445 AO-TriggerSampleRate": "100000", "640 Shutter-IOChannel": "Dev1/ao14", "555 AO-Sequenceable": "1", "520 AO-Name": "AnalogIO", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "470 AO-Block voltage": "false", "COM1-Verbose": "1", "COM9-AnswerTimeout": "10000.0000", "528 Shutter-Name": "AnalogIO", "PRIME-Trigger Timeout (secs)": "10", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "NextFrame": 0, "470 AO-TriggerSequenceLength": "1000", "PRIME-TransposeXY": "0", "445 AO-TriggerSequenceLength": "1000", "COM1-Name": "COM1", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "COM9-DataBits": "8", "COM1-AnswerTimeout": "500.0000", "Camera": "", "TE2000-Version": "V2.50-V2.50-V2.10", "528 Shutter-Volts": "0.0000", "XLIGHT Hub-Name": "XLIGHT Hub", "PIEZO-Description": "Ludl stage driver adapter", "PositionName": "Pos29", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSampleRate": "100000", "PRIME-FanSpeedSetpoint": "High", "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "405 AO-TriggerSampleRate": "100000", "470 Shutter-Block voltage": "false", "470-State": "0", "COM3-Parity": "None", "405 AO-Volts": "0.0000", "PRIME-TransposeMirrorY": "0", "PRIME-ChipName": "GS144BSI", "470 AO-Volts": "0.0000", "PRIME-TransposeMirrorX": "0", "445 Shutter-SupportsTriggering": "0", "470 Shutter-SupportsTriggering": "0", "XLIGHT Hub-Description": "XLIGHT hub", "PRIME-PreampOffLimit": "10000", "470 AO-Sequenceable": "1", "528 Shutter-MaxVolts": "5.0000", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "Core-XYStage": "XYStage", "470 AO-TriggerSampleRate": "100000", "PRIME-Timing-PreTriggerDelayNs": "24672000.0000", "528-DA Device": "528 Shutter", "Focus-Description": "Nikon TE2000 focus adapter", "FilterBlock-State": "5", "520 AO-Demo": "No", "COM3-DelayBetweenCharsMs": "0.0000", "470 Shutter-Volts": "0.0000", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "PRIME-PP 2 THRESHOLD": "125", "PRIME-PP 4 THRESHOLD": "75", "XYStage-Acceleration": "4.0000", "470 Shutter-Demo": "No", "PRIME-Name": "PMPCIECam00", "640-DA Device": "640 Shutter", "405 AO-Block voltage": "false", "ElapsedTime-ms": 1220425, "PRIME-Offset": "100", "445 AO-IOChannel": "Dev1/ao1", "640 AO-MaxVolts": "5.0000", "PositionIndex": 29, "PRIME-PP 1 ENABLED": "Yes", "COM1-BaudRate": "9600", "640-State": "0", "COM3-Verbose": "1", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "LudlController-TransmissionDelay": "0", "PRIME-FullWellCapacity": "62000", "PRIME-SMARTStreamingEnabled": "No", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "640 Shutter-Description": "NI DAC", "528-Description": "DA device that is used as a shutter", "COM4-Description": "Serial port driver (boost:asio)", "405 Shutter-Description": "NI DAC", "TE2000-Name": "TE2000", "528 Shutter-Description": "NI DAC", "COM9-Description": "Serial port driver (boost:asio)", "LudlController-Name": "LudlController", "445 Shutter-TriggerSequenceLength": "1000", "PRIME-SerialNumber": "A18B203008", "445 AO-Volts": "0.0000", "PRIME-X-dimension": "1200", "PRIME-ShutterCloseDelay": "0", "XLIGHT Disk slider-State": "2", "Position": "Default", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 AO-IOChannel": "Dev1/ao5", "520 AO-IOChannel": "Dev1/ao4", "528 AO-TriggerSequenceLength": "1000", "405 Shutter-MaxVolts": "5.0000", "470-DA Device": "470 Shutter", "PRIME-FTCapable": "No", "Core-ChannelGroup": "Channels", "555 AO-SupportsTriggering": "0", "PRIME-Y-dimension": "1200", "Frame": 0, "555 Shutter-Sequenceable": "1", "PRIME-Timing-ExposureTimeNs": "499998000.0000", "Core-ImageProcessor": "", "528-State": "0", "520 AO-Block voltage": "false", "XLIGHT Excitation Wheel-Label": "Excitation-2", "640 Shutter-Demo": "No", "COM3-StopBits": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "520 Shutter-IOChannel": "Dev1/ao11", "Width": 1200, "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-SupportsTriggering": "0", "640 AO-TriggerSampleRate": "100000", "520-Description": "DA device that is used as a shutter", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "405 Shutter-Sequenceable": "1", "405 Shutter-SupportsTriggering": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-TriggerSampleRate": "100000", "640 Shutter-MaxVolts": "5.0000", "555 AO-MaxVolts": "5.0000", "520 AO-TriggerSequenceLength": "1000", "COM3-BaudRate": "9600", "COM9-BaudRate": "9600", "Vincent-D3-Channel #": "Ch. #1", "PRIME-PMode": "Normal", "OpticalPath-Label": "Left(100)", "BitDepth": 16, "555 AO-TriggerSequenceLength": "1000", "445 Shutter-Description": "NI DAC", "405 AO-Sequenceable": "1", "PRIME-AcquisitionMethod": "Callbacks", "555 AO-Name": "AnalogIO", "PRIME-ShutterMode": "Pre-Exposure", "COM3-Handshaking": "Off", "445 AO-SupportsTriggering": "0", "ROI": "0-0-1200-1200", "COM9-Verbose": "1", "PRIME-Color": "OFF", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "PRIME-ShutterOpenDelay": "0", "COM1-Parity": "None", "Slice": 8, "SlicePosition": 30, "COM9-DelayBetweenCharsMs": "0.0000", "555-Name": "DA Shutter", "PIEZO-LudlSingleAxisName": "B", "XLIGHT Emission Wheel-Label": "Empty", "640 Shutter-Name": "AnalogIO", "640 AO-Sequenceable": "1", "520 Shutter-Demo": "No", "528 Shutter-Demo": "No", "470-Name": "DA Shutter", "520-DA Device": "520 Shutter", "XYStage-Description": "Ludl XY stage driver adapter", "445 Shutter-MinVolts": "0.0000", "528 AO-TriggerSampleRate": "100000", "405 AO-MinVolts": "0.0000", "405 Shutter-TriggerSequenceLength": "1000", "Height": 1200, "555 AO-Block voltage": "false", "640 Shutter-Volts": "0.0000", "PixelType": "GRAY16", "445 Shutter-Block voltage": "false", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "520 Shutter-TriggerSequenceLength": "1000", "COM9-Name": "COM9", "Core-SLM": "", "555 AO-Volts": "0.0000", "555 Shutter-Block voltage": "false", "XYStage-Speed": "1800.0000", "520 AO-MaxVolts": "5.0000", "528 Shutter-SupportsTriggering": "0", "COM1-Description": "Serial port driver (boost:asio)", "PRIME-MetadataEnabled": "No", "520 Shutter-MinVolts": "0.0000", "470 Shutter-Sequenceable": "1", "PRIME-PreampDelay": "0", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-MinVolts": "0.0000", "ChannelIndex": 4, "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Core-AutoShutter": "1", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "Exposure-ms": 20, "PRIME-PP 2 ENABLED": "Yes", "640 Shutter-TriggerSampleRate": "100000", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "470 Shutter-IOChannel": "Dev1/ao10", "405 AO-Demo": "No", "PixelSizeUm": 1.1, "470 AO-Demo": "No", "470 Shutter-MaxVolts": "5.0000", "Vincent-D3-Name": "Vincent-D3", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FileName": "img_000000000_P-DIC_008.tif", "LudlController-Reset": "Operate", "PRIME-Exposure": "20.00", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "445-Name": "DA Shutter", "555 Shutter-Name": "AnalogIO", "520 Shutter-TriggerSampleRate": "100000", "445 Shutter-IOChannel": "Dev1/ao9", "PRIME-TransposeCorrection": "0", "XYStage-StepSize": "0.1000", "445-Description": "DA device that is used as a shutter", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 AO-IOChannel": "Dev1/ao7", "520 Shutter-Name": "AnalogIO", "COM3-DataBits": "8", "640 AO-Volts": "0.0000", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "555 Shutter-IOChannel": "Dev1/ao13", "WaitInterval": null, "520 Shutter-Sequenceable": "1", "YPositionUm": -180, "445-State": "0", "445-DA Device": "445 Shutter", "PRIME-CircularBufferAutoSize": "ON", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "555 AO-TriggerSampleRate": "100000", "445 Shutter-Volts": "0.0000", "XLIGHT Hub-Port": "COM3", "Core-AutoFocus": "", "COM4-Handshaking": "Off", "528 Shutter-MinVolts": "0.0000", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "445 Shutter-Name": "AnalogIO", "528 Shutter-Sequenceable": "1", "XYStage-TransposeMirrorX": "0", "XYStage-TransposeMirrorY": "0", "PRIME-ReadoutRate": "100MHz 16bit", "XYStage-Name": "Stage", "640 AO-SupportsTriggering": "0", "640-Name": "DA Shutter", "640 Shutter-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "640 AO-Block voltage": "false", "445 Shutter-Sequenceable": "1", "470 AO-MaxVolts": "5.0000", "COM4-BaudRate": "9600", "470-Description": "DA device that is used as a shutter", "520 Shutter-Description": "NI DAC", "PRIME-CCDTemperature": "-14.8500", "Time": "2019-12-18 23:11:20 -0500", "640 AO-TriggerSequenceLength": "1000", "470 AO-Name": "AnalogIO", "XLIGHT Disk slider-Label": "Disk Pos 40um", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "Binning": "1x1", "TE2000-ModelType": "1", "COM3-AnswerTimeout": "500.0000", "555 Shutter-SupportsTriggering": "0", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "TE2000-Port": "COM4", "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Description": "NI DAC", "COM4-AnswerTimeout": "500.0000", "COM1-DelayBetweenCharsMs": "0.0000", "FilterBlock-Label": "Confocal", "445 AO-Name": "AnalogIO", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-Timing-ReadoutTimeNs": "24672000.0000", "528 AO-Demo": "No", "520 AO-Sequenceable": "1", "555-Description": "DA device that is used as a shutter", "405-DA Device": "405 Shutter", "405 AO-TriggerSequenceLength": "1000", "640 AO-MinVolts": "0.0000", "640 Shutter-Block voltage": "false", "FrameIndex": 0, "PRIME-Port": "CMOS", "555 AO-MinVolts": "0.0000", "COM3-Description": "Serial port driver (boost:asio)", "528 AO-Description": "NI DAC", "555 AO-IOChannel": "Dev1/ao6", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "555 AO-TriggerInputLine": "/Dev1/PFI0", "PRIME-ActualInterval-ms": "390.5200", "LudlController-Port": "COM9", "445 Shutter-Demo": "No", "PRIME-Trigger-Expose Out-Mux": "1", "SliceIndex": 8, "PRIME-CCDTemperatureSetPoint": "-15.0000", "405 Shutter-MinVolts": "0.0000", "PRIME-ExposeOutMode": "First Row", "AxisPositions": null, "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Volts": "0.0000", "528-Name": "DA Shutter", "555-State": "0", "XLIGHT Dichroic-Label": "Di03-R635-t1", "445 AO-Sequenceable": "1", "405-Name": "DA Shutter", "405 AO-IOChannel": "Dev1/ao0", "Core-Camera": "PRIME", "Focus-Name": "Focus", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "405 Shutter-IOChannel": "Dev1/ao8", "640 AO-Demo": "No", "640 Shutter-SupportsTriggering": "0", "445 AO-Demo": "No", "555-DA Device": "555 Shutter", "470 Shutter-Description": "NI DAC", "528 AO-Volts": "0.0000", "COM1-DataBits": "8", "Vincent-D3-Address": "x", "520 AO-SupportsTriggering": "0", "405-Description": "DA device that is used as a shutter", "640 Shutter-MinVolts": "0.0000", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "405 AO-Name": "AnalogIO", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "640 AO-TriggerInputLine": "/Dev1/PFI0", "405 AO-TriggerInputLine": "/Dev1/PFI0", "640-Description": "DA device that is used as a shutter", "445 Shutter-TriggerSampleRate": "100000", "470 Shutter-TriggerSequenceLength": "1000", "COM1-StopBits": "1", "520 Shutter-SupportsTriggering": "0", "405-State": "0", "Core-Initialize": "1", "XLIGHT Emission Wheel-State": "3", "520 AO-Description": "NI DAC", "528 AO-TriggerInputLine": "/Dev1/PFI0", "445 Shutter-MaxVolts": "5.0000", "COM9-Handshaking": "Off", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "XLIGHT Spin motor-Label": "On (spinning)", "PRIME-PP 5 ENABLED": "No", "UUID": "d1c497d2-a841-48aa-bf0a-54807927a02a", "PRIME-TriggerMode": "Internal Trigger", "COM1-Handshaking": "Off", "PRIME-Timing-PostTriggerDelayNs": "49344000.0000", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "Focus-Position": "17741.5000", "528 Shutter-Block voltage": "false", "Channel": "P-DIC", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "555 Shutter-Demo": "No", "405 Shutter-Demo": "No", "528 AO-Name": "AnalogIO", "520-State": "0", "Core-Galvo": "", "405 Shutter-TriggerSampleRate": "100000", "Focus-FocusStepSize_(nm)": "100", "PRIME-PixelType": "16bit", "COM4-DataBits": "8", "470 AO-IOChannel": "Dev1/ao2", "520 AO-MinVolts": "0.0000", "COM9-Parity": "None", "PRIME-CircularBufferEnabled": "ON", "PRIME-PP 6 Reset": "No", "640 AO-Description": "NI DAC", "Source": "PRIME", "445 AO-MinVolts": "0.0000", "PIEZO-Name": "Stage", "445 AO-Description": "NI DAC", "PRIME-CircularBufferFrameCount": "46", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "520 AO-Volts": "0.0000", "528 Shutter-TriggerSampleRate": "100000", "XPositionUm": 15712.1, "OpticalPath-State": "4", "PRIME-PP 3 THRESHOLD": "80", "PRIME-PVCAM Version": "3.7.1", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Gain": "1-HDR", "640 Shutter-TriggerSequenceLength": "1000", "528 AO-SupportsTriggering": "0", "445 AO-TriggerInputLine": "/Dev1/PFI0", "OpticalPath-Name": "OpticalPath", "PRIME-PP 4 ENABLED": "Yes", "PRIME-PVCAM Adapter Version": "1.3.29", "COM3-Name": "COM3", "405 AO-MaxVolts": "5.0000", "520 Shutter-Block voltage": "false", "PRIME-Timing-ClearingTimeNs": "49344000.0000", "Core-Focus": "PIEZO", "528 AO-Block voltage": "false", "TE2000-Description": "Nikon TE2000 hub", "528 AO-MinVolts": "0.0000", "COM4-Parity": "None", "Summary": { "Slices": 11, "ChContrastMin": [ 0, 0, 0, 0, 0, 0 ], "PixelAspect": 1, "SlicesFirst": true, "Interval_ms": 0, "Time": "2019-12-18 23:10:50 -0500", "Prefix": "seq_5", "InitialPositionList": [ { "Label": "Pos0", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.21], "Focus": [17741.5], "XYStage": [ 44542.200000000004, 31636.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos1", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.22], "Focus": [17741.4], "XYStage": [ 43801.100000000006, 31637.9 ] }, "GridColumnIndex": 0 }, { "Label": "Pos2", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.5], "XYStage": [ 38561.3, 32040.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos3", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.185], "Focus": [17741.3], "XYStage": [ 37698.4, 32041.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos4", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.16], "Focus": [17741.5], "XYStage": [ 32375.4, 31758.9 ] }, "GridColumnIndex": 0 }, { "Label": "Pos5", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.17000000000002], "Focus": [17741.3], "XYStage": [ 31770.7, 31758.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos6", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.4], "XYStage": [ 26536.800000000003, 31068.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos7", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.205], "Focus": [17741.4], "XYStage": [ 25420.2, 31068.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos8", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.85], "Focus": [17741.3], "XYStage": [ 21059, 31508 ] }, "GridColumnIndex": 0 }, { "Label": "Pos9", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.82], "Focus": [17741.3], "XYStage": [ 19731.9, 31506.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos10", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.79], "Focus": [17741.4], "XYStage": [ 14528.5, 32007.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos11", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.83], "Focus": [17741.2], "XYStage": [ 13830.300000000001, 32009.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos12", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.79], "Focus": [17741.3], "XYStage": [ 8775.300000000001, 32017.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos13", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.815], "Focus": [17741.3], "XYStage": [ 8109.200000000001, 32008.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos14", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.805], "Focus": [17741.3], "XYStage": [ 3483.3, 31908.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos15", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.82], "Focus": [17741.2], "XYStage": [ 2686.8, 31903.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos16", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [211.48000000000002], "Focus": [17741.1], "XYStage": [ -1978.9, 31973.600000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos17", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [211.51500000000001], "Focus": [17741.1], "XYStage": [ -2957.8, 31982.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos18", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.195], "Focus": [17741.5], "XYStage": [ 47747.4, -576.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos19", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.23000000000002], "Focus": [17741.6], "XYStage": [ 47053.9, -576.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos20", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.205], "Focus": [17741.4], "XYStage": [ 41871.200000000004, -479.8 ] }, "GridColumnIndex": 0 }, { "Label": "Pos21", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.4], "XYStage": [ 40882.600000000006, -392 ] }, "GridColumnIndex": 0 }, { "Label": "Pos22", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.24], "Focus": [17741.4], "XYStage": [ 35905.6, -135.3 ] }, "GridColumnIndex": 0 }, { "Label": "Pos23", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.195], "Focus": [17741.5], "XYStage": [ 34990.9, -132.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos24", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.46], "Focus": [17741.4], "XYStage": [ 29678.100000000002, -958.1 ] }, "GridColumnIndex": 0 }, { "Label": "Pos25", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.44], "Focus": [17741.5], "XYStage": [ 28983.600000000002, -939.4000000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos26", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.475], "Focus": [17741.5], "XYStage": [ 23019.7, -122 ] }, "GridColumnIndex": 0 }, { "Label": "Pos27", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.44], "Focus": [17741.7], "XYStage": [ 22428, -116.80000000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos28", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.46], "Focus": [17741.7], "XYStage": [ 16364.900000000001, -181.10000000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos29", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.48000000000002], "Focus": [17741.5], "XYStage": [ 15712.1, -180 ] }, "GridColumnIndex": 0 }, { "Label": "Pos30", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.45000000000002], "Focus": [17741.7], "XYStage": [ 10346.1, 77.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos31", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.49], "Focus": [17741.4], "XYStage": [ 9755.5, 85.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos32", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.475], "Focus": [17741.5], "XYStage": [ 4775.5, 293.90000000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos33", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.445], "Focus": [17741.4], "XYStage": [ 3954.9, 293.90000000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos34", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.455], "Focus": [17741.4], "XYStage": [ -1324.9, 545.6 ] }, "GridColumnIndex": 0 }, { "Label": "Pos35", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.445], "Focus": [17741.3], "XYStage": [ -2097, 576.6 ] }, "GridColumnIndex": 0 } ], "z-step_um": 10, "GridRow": 0, "BitDepth": 16, "Depth": 2, "Channels": 6, "Source": "Micro-Manager", "ChColors": [ -16711681, -256, -65281, -1, -1, -16776961 ], "MetadataVersion": 10, "KeepShutterOpenChannels": true, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536, 65536 ], "CustomIntervals_ms": [], "Height": 1200, "MicroManagerVersion": "1.4.23 20180219", "GridColumn": 0, "UUID": "67598d21-5db5-4842-ab60-f506f7b42c7c", "PixelType": "GRAY16", "Width": 1200, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\Yan\\20191218-YC113-S7S8-miseq", "Frames": 1, "Comment": "", "UserName": "Nikon", "PixelSize_um": 1.1, "ROI": [ 0, 0, 1200, 1200 ], "Date": "2019-12-18", "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC", "P-DAPI-high" ], "KeepShutterOpenSlices": true, "IJType": 1, "ComputerName": "ZadorConfocal2", "Positions": 36, "PositionIndex": 29, "CameraTimeout": 20000, "TimeFirst": false }, "640 AO-Name": "AnalogIO", "Vincent-D3-Command": "Close" }, "FrameKey-0-4-9": { "COM4-StopBits": "1", "528 AO-MaxVolts": "5.0000", "XLIGHT Excitation Wheel-State": "1", "470 Shutter-Name": "AnalogIO", "Core-Shutter": "Vincent-D3", "555 AO-Demo": "No", "XLIGHT Dichroic-State": "2", "XYStage-StepSize-Y": "0.1000", "ZPositionUm": 258.48, "XYStage-StepSize-X": "0.1000", "PRIME-FirmwareVersion": "20.28", "PRIME-ClearCycles": "2", "520-Name": "DA Shutter", "405 Shutter-Name": "AnalogIO", "PIEZO-StepSize": "0.0050", "PRIME-ClearMode": "Pre-Exposure", "470 AO-MinVolts": "0.0000", "405 AO-SupportsTriggering": "1", "CameraChannelIndex": 0, "XYStage-StartSpeed": "500.0000", "COM9-StopBits": "2", "405 AO-Description": "NI DAC", "COM4-DelayBetweenCharsMs": "0.0000", "445 AO-Block voltage": "false", "520 AO-TriggerInputLine": "/Dev1/PFI0", "555 AO-Description": "NI DAC", "520 AO-TriggerSampleRate": "100000", "COM4-Verbose": "1", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM4-Name": "COM4", "405 Shutter-Volts": "0.0000", "Vincent-D3-Port": "COM1", "PRIME-PP 4 MIN ADU AFFECTED": "200", "470 Shutter-MinVolts": "0.0000", "PRIME-PP 3 ENABLED": "Yes", "470 AO-Description": "NI DAC", "PRIME-Description": "PVCAM API device adapter", "520 Shutter-MaxVolts": "5.0000", "520 Shutter-Volts": "0.0000", "528 AO-Sequenceable": "1", "PRIME-Binning": "1x1", "555 Shutter-MaxVolts": "5.0000", "528 Shutter-TriggerSequenceLength": "1000", "405 Shutter-Block voltage": "false", "XLIGHT Spin motor-State": "1", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "Vincent-D3-Time to open (ms)": "35.0000", "Vincent-D3-Time to close (ms)": "35.0000", "445 AO-TriggerSampleRate": "100000", "640 Shutter-IOChannel": "Dev1/ao14", "555 AO-Sequenceable": "1", "520 AO-Name": "AnalogIO", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "470 AO-Block voltage": "false", "COM1-Verbose": "1", "COM9-AnswerTimeout": "10000.0000", "528 Shutter-Name": "AnalogIO", "PRIME-Trigger Timeout (secs)": "10", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "NextFrame": 0, "470 AO-TriggerSequenceLength": "1000", "PRIME-TransposeXY": "0", "445 AO-TriggerSequenceLength": "1000", "COM1-Name": "COM1", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "COM9-DataBits": "8", "COM1-AnswerTimeout": "500.0000", "Camera": "", "TE2000-Version": "V2.50-V2.50-V2.10", "528 Shutter-Volts": "0.0000", "XLIGHT Hub-Name": "XLIGHT Hub", "PIEZO-Description": "Ludl stage driver adapter", "PositionName": "Pos29", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSampleRate": "100000", "PRIME-FanSpeedSetpoint": "High", "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "405 AO-TriggerSampleRate": "100000", "470 Shutter-Block voltage": "false", "470-State": "0", "COM3-Parity": "None", "405 AO-Volts": "0.0000", "PRIME-TransposeMirrorY": "0", "PRIME-ChipName": "GS144BSI", "470 AO-Volts": "0.0000", "PRIME-TransposeMirrorX": "0", "445 Shutter-SupportsTriggering": "0", "470 Shutter-SupportsTriggering": "0", "XLIGHT Hub-Description": "XLIGHT hub", "PRIME-PreampOffLimit": "10000", "470 AO-Sequenceable": "1", "528 Shutter-MaxVolts": "5.0000", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "Core-XYStage": "XYStage", "470 AO-TriggerSampleRate": "100000", "PRIME-Timing-PreTriggerDelayNs": "24672000.0000", "528-DA Device": "528 Shutter", "Focus-Description": "Nikon TE2000 focus adapter", "FilterBlock-State": "5", "520 AO-Demo": "No", "COM3-DelayBetweenCharsMs": "0.0000", "470 Shutter-Volts": "0.0000", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "PRIME-PP 2 THRESHOLD": "125", "PRIME-PP 4 THRESHOLD": "75", "XYStage-Acceleration": "4.0000", "470 Shutter-Demo": "No", "PRIME-Name": "PMPCIECam00", "640-DA Device": "640 Shutter", "405 AO-Block voltage": "false", "ElapsedTime-ms": 1220702, "PRIME-Offset": "100", "445 AO-IOChannel": "Dev1/ao1", "640 AO-MaxVolts": "5.0000", "PositionIndex": 29, "PRIME-PP 1 ENABLED": "Yes", "COM1-BaudRate": "9600", "640-State": "0", "COM3-Verbose": "1", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "LudlController-TransmissionDelay": "0", "PRIME-FullWellCapacity": "62000", "PRIME-SMARTStreamingEnabled": "No", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "640 Shutter-Description": "NI DAC", "528-Description": "DA device that is used as a shutter", "COM4-Description": "Serial port driver (boost:asio)", "405 Shutter-Description": "NI DAC", "TE2000-Name": "TE2000", "528 Shutter-Description": "NI DAC", "COM9-Description": "Serial port driver (boost:asio)", "LudlController-Name": "LudlController", "445 Shutter-TriggerSequenceLength": "1000", "PRIME-SerialNumber": "A18B203008", "445 AO-Volts": "0.0000", "PRIME-X-dimension": "1200", "PRIME-ShutterCloseDelay": "0", "XLIGHT Disk slider-State": "2", "Position": "Default", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 AO-IOChannel": "Dev1/ao5", "520 AO-IOChannel": "Dev1/ao4", "528 AO-TriggerSequenceLength": "1000", "405 Shutter-MaxVolts": "5.0000", "470-DA Device": "470 Shutter", "PRIME-FTCapable": "No", "Core-ChannelGroup": "Channels", "555 AO-SupportsTriggering": "0", "PRIME-Y-dimension": "1200", "Frame": 0, "555 Shutter-Sequenceable": "1", "PRIME-Timing-ExposureTimeNs": "499998000.0000", "Core-ImageProcessor": "", "528-State": "0", "520 AO-Block voltage": "false", "XLIGHT Excitation Wheel-Label": "Excitation-2", "640 Shutter-Demo": "No", "COM3-StopBits": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "520 Shutter-IOChannel": "Dev1/ao11", "Width": 1200, "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-SupportsTriggering": "0", "640 AO-TriggerSampleRate": "100000", "520-Description": "DA device that is used as a shutter", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "405 Shutter-Sequenceable": "1", "405 Shutter-SupportsTriggering": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-TriggerSampleRate": "100000", "640 Shutter-MaxVolts": "5.0000", "555 AO-MaxVolts": "5.0000", "520 AO-TriggerSequenceLength": "1000", "COM3-BaudRate": "9600", "COM9-BaudRate": "9600", "Vincent-D3-Channel #": "Ch. #1", "PRIME-PMode": "Normal", "OpticalPath-Label": "Left(100)", "BitDepth": 16, "555 AO-TriggerSequenceLength": "1000", "445 Shutter-Description": "NI DAC", "405 AO-Sequenceable": "1", "PRIME-AcquisitionMethod": "Callbacks", "555 AO-Name": "AnalogIO", "PRIME-ShutterMode": "Pre-Exposure", "COM3-Handshaking": "Off", "445 AO-SupportsTriggering": "0", "ROI": "0-0-1200-1200", "COM9-Verbose": "1", "PRIME-Color": "OFF", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "PRIME-ShutterOpenDelay": "0", "COM1-Parity": "None", "Slice": 9, "SlicePosition": 40, "COM9-DelayBetweenCharsMs": "0.0000", "555-Name": "DA Shutter", "PIEZO-LudlSingleAxisName": "B", "XLIGHT Emission Wheel-Label": "Empty", "640 Shutter-Name": "AnalogIO", "640 AO-Sequenceable": "1", "520 Shutter-Demo": "No", "528 Shutter-Demo": "No", "470-Name": "DA Shutter", "520-DA Device": "520 Shutter", "XYStage-Description": "Ludl XY stage driver adapter", "445 Shutter-MinVolts": "0.0000", "528 AO-TriggerSampleRate": "100000", "405 AO-MinVolts": "0.0000", "405 Shutter-TriggerSequenceLength": "1000", "Height": 1200, "555 AO-Block voltage": "false", "640 Shutter-Volts": "0.0000", "PixelType": "GRAY16", "445 Shutter-Block voltage": "false", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "520 Shutter-TriggerSequenceLength": "1000", "COM9-Name": "COM9", "Core-SLM": "", "555 AO-Volts": "0.0000", "555 Shutter-Block voltage": "false", "XYStage-Speed": "1800.0000", "520 AO-MaxVolts": "5.0000", "528 Shutter-SupportsTriggering": "0", "COM1-Description": "Serial port driver (boost:asio)", "PRIME-MetadataEnabled": "No", "520 Shutter-MinVolts": "0.0000", "470 Shutter-Sequenceable": "1", "PRIME-PreampDelay": "0", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-MinVolts": "0.0000", "ChannelIndex": 4, "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Core-AutoShutter": "1", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "Exposure-ms": 20, "PRIME-PP 2 ENABLED": "Yes", "640 Shutter-TriggerSampleRate": "100000", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "470 Shutter-IOChannel": "Dev1/ao10", "405 AO-Demo": "No", "PixelSizeUm": 1.1, "470 AO-Demo": "No", "470 Shutter-MaxVolts": "5.0000", "Vincent-D3-Name": "Vincent-D3", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FileName": "img_000000000_P-DIC_009.tif", "LudlController-Reset": "Operate", "PRIME-Exposure": "20.00", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "445-Name": "DA Shutter", "555 Shutter-Name": "AnalogIO", "520 Shutter-TriggerSampleRate": "100000", "445 Shutter-IOChannel": "Dev1/ao9", "PRIME-TransposeCorrection": "0", "XYStage-StepSize": "0.1000", "445-Description": "DA device that is used as a shutter", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 AO-IOChannel": "Dev1/ao7", "520 Shutter-Name": "AnalogIO", "COM3-DataBits": "8", "640 AO-Volts": "0.0000", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "555 Shutter-IOChannel": "Dev1/ao13", "WaitInterval": null, "520 Shutter-Sequenceable": "1", "YPositionUm": -180, "445-State": "0", "445-DA Device": "445 Shutter", "PRIME-CircularBufferAutoSize": "ON", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "555 AO-TriggerSampleRate": "100000", "445 Shutter-Volts": "0.0000", "XLIGHT Hub-Port": "COM3", "Core-AutoFocus": "", "COM4-Handshaking": "Off", "528 Shutter-MinVolts": "0.0000", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "445 Shutter-Name": "AnalogIO", "528 Shutter-Sequenceable": "1", "XYStage-TransposeMirrorX": "0", "XYStage-TransposeMirrorY": "0", "PRIME-ReadoutRate": "100MHz 16bit", "XYStage-Name": "Stage", "640 AO-SupportsTriggering": "0", "640-Name": "DA Shutter", "640 Shutter-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "640 AO-Block voltage": "false", "445 Shutter-Sequenceable": "1", "470 AO-MaxVolts": "5.0000", "COM4-BaudRate": "9600", "470-Description": "DA device that is used as a shutter", "520 Shutter-Description": "NI DAC", "PRIME-CCDTemperature": "-14.8500", "Time": "2019-12-18 23:11:20 -0500", "640 AO-TriggerSequenceLength": "1000", "470 AO-Name": "AnalogIO", "XLIGHT Disk slider-Label": "Disk Pos 40um", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "Binning": "1x1", "TE2000-ModelType": "1", "COM3-AnswerTimeout": "500.0000", "555 Shutter-SupportsTriggering": "0", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "TE2000-Port": "COM4", "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Description": "NI DAC", "COM4-AnswerTimeout": "500.0000", "COM1-DelayBetweenCharsMs": "0.0000", "FilterBlock-Label": "Confocal", "445 AO-Name": "AnalogIO", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-Timing-ReadoutTimeNs": "24672000.0000", "528 AO-Demo": "No", "520 AO-Sequenceable": "1", "555-Description": "DA device that is used as a shutter", "405-DA Device": "405 Shutter", "405 AO-TriggerSequenceLength": "1000", "640 AO-MinVolts": "0.0000", "640 Shutter-Block voltage": "false", "FrameIndex": 0, "PRIME-Port": "CMOS", "555 AO-MinVolts": "0.0000", "COM3-Description": "Serial port driver (boost:asio)", "528 AO-Description": "NI DAC", "555 AO-IOChannel": "Dev1/ao6", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "555 AO-TriggerInputLine": "/Dev1/PFI0", "PRIME-ActualInterval-ms": "390.5200", "LudlController-Port": "COM9", "445 Shutter-Demo": "No", "PRIME-Trigger-Expose Out-Mux": "1", "SliceIndex": 9, "PRIME-CCDTemperatureSetPoint": "-15.0000", "405 Shutter-MinVolts": "0.0000", "PRIME-ExposeOutMode": "First Row", "AxisPositions": null, "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Volts": "0.0000", "528-Name": "DA Shutter", "555-State": "0", "XLIGHT Dichroic-Label": "Di03-R635-t1", "445 AO-Sequenceable": "1", "405-Name": "DA Shutter", "405 AO-IOChannel": "Dev1/ao0", "Core-Camera": "PRIME", "Focus-Name": "Focus", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "405 Shutter-IOChannel": "Dev1/ao8", "640 AO-Demo": "No", "640 Shutter-SupportsTriggering": "0", "445 AO-Demo": "No", "555-DA Device": "555 Shutter", "470 Shutter-Description": "NI DAC", "528 AO-Volts": "0.0000", "COM1-DataBits": "8", "Vincent-D3-Address": "x", "520 AO-SupportsTriggering": "0", "405-Description": "DA device that is used as a shutter", "640 Shutter-MinVolts": "0.0000", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "405 AO-Name": "AnalogIO", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "640 AO-TriggerInputLine": "/Dev1/PFI0", "405 AO-TriggerInputLine": "/Dev1/PFI0", "640-Description": "DA device that is used as a shutter", "445 Shutter-TriggerSampleRate": "100000", "470 Shutter-TriggerSequenceLength": "1000", "COM1-StopBits": "1", "520 Shutter-SupportsTriggering": "0", "405-State": "0", "Core-Initialize": "1", "XLIGHT Emission Wheel-State": "3", "520 AO-Description": "NI DAC", "528 AO-TriggerInputLine": "/Dev1/PFI0", "445 Shutter-MaxVolts": "5.0000", "COM9-Handshaking": "Off", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "XLIGHT Spin motor-Label": "On (spinning)", "PRIME-PP 5 ENABLED": "No", "UUID": "c560227b-6201-4853-af34-172979454ba7", "PRIME-TriggerMode": "Internal Trigger", "COM1-Handshaking": "Off", "PRIME-Timing-PostTriggerDelayNs": "49344000.0000", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "Focus-Position": "17741.5000", "528 Shutter-Block voltage": "false", "Channel": "P-DIC", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "555 Shutter-Demo": "No", "405 Shutter-Demo": "No", "528 AO-Name": "AnalogIO", "520-State": "0", "Core-Galvo": "", "405 Shutter-TriggerSampleRate": "100000", "Focus-FocusStepSize_(nm)": "100", "PRIME-PixelType": "16bit", "COM4-DataBits": "8", "470 AO-IOChannel": "Dev1/ao2", "520 AO-MinVolts": "0.0000", "COM9-Parity": "None", "PRIME-CircularBufferEnabled": "ON", "PRIME-PP 6 Reset": "No", "640 AO-Description": "NI DAC", "Source": "PRIME", "445 AO-MinVolts": "0.0000", "PIEZO-Name": "Stage", "445 AO-Description": "NI DAC", "PRIME-CircularBufferFrameCount": "46", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "520 AO-Volts": "0.0000", "528 Shutter-TriggerSampleRate": "100000", "XPositionUm": 15712.1, "OpticalPath-State": "4", "PRIME-PP 3 THRESHOLD": "80", "PRIME-PVCAM Version": "3.7.1", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Gain": "1-HDR", "640 Shutter-TriggerSequenceLength": "1000", "528 AO-SupportsTriggering": "0", "445 AO-TriggerInputLine": "/Dev1/PFI0", "OpticalPath-Name": "OpticalPath", "PRIME-PP 4 ENABLED": "Yes", "PRIME-PVCAM Adapter Version": "1.3.29", "COM3-Name": "COM3", "405 AO-MaxVolts": "5.0000", "520 Shutter-Block voltage": "false", "PRIME-Timing-ClearingTimeNs": "49344000.0000", "Core-Focus": "PIEZO", "528 AO-Block voltage": "false", "TE2000-Description": "Nikon TE2000 hub", "528 AO-MinVolts": "0.0000", "COM4-Parity": "None", "Summary": { "Slices": 11, "ChContrastMin": [ 0, 0, 0, 0, 0, 0 ], "PixelAspect": 1, "SlicesFirst": true, "Interval_ms": 0, "Time": "2019-12-18 23:10:50 -0500", "Prefix": "seq_5", "InitialPositionList": [ { "Label": "Pos0", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.21], "Focus": [17741.5], "XYStage": [ 44542.200000000004, 31636.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos1", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.22], "Focus": [17741.4], "XYStage": [ 43801.100000000006, 31637.9 ] }, "GridColumnIndex": 0 }, { "Label": "Pos2", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.5], "XYStage": [ 38561.3, 32040.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos3", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.185], "Focus": [17741.3], "XYStage": [ 37698.4, 32041.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos4", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.16], "Focus": [17741.5], "XYStage": [ 32375.4, 31758.9 ] }, "GridColumnIndex": 0 }, { "Label": "Pos5", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.17000000000002], "Focus": [17741.3], "XYStage": [ 31770.7, 31758.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos6", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.4], "XYStage": [ 26536.800000000003, 31068.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos7", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.205], "Focus": [17741.4], "XYStage": [ 25420.2, 31068.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos8", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.85], "Focus": [17741.3], "XYStage": [ 21059, 31508 ] }, "GridColumnIndex": 0 }, { "Label": "Pos9", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.82], "Focus": [17741.3], "XYStage": [ 19731.9, 31506.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos10", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.79], "Focus": [17741.4], "XYStage": [ 14528.5, 32007.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos11", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.83], "Focus": [17741.2], "XYStage": [ 13830.300000000001, 32009.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos12", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.79], "Focus": [17741.3], "XYStage": [ 8775.300000000001, 32017.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos13", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.815], "Focus": [17741.3], "XYStage": [ 8109.200000000001, 32008.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos14", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.805], "Focus": [17741.3], "XYStage": [ 3483.3, 31908.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos15", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.82], "Focus": [17741.2], "XYStage": [ 2686.8, 31903.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos16", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [211.48000000000002], "Focus": [17741.1], "XYStage": [ -1978.9, 31973.600000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos17", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [211.51500000000001], "Focus": [17741.1], "XYStage": [ -2957.8, 31982.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos18", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.195], "Focus": [17741.5], "XYStage": [ 47747.4, -576.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos19", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.23000000000002], "Focus": [17741.6], "XYStage": [ 47053.9, -576.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos20", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.205], "Focus": [17741.4], "XYStage": [ 41871.200000000004, -479.8 ] }, "GridColumnIndex": 0 }, { "Label": "Pos21", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.4], "XYStage": [ 40882.600000000006, -392 ] }, "GridColumnIndex": 0 }, { "Label": "Pos22", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.24], "Focus": [17741.4], "XYStage": [ 35905.6, -135.3 ] }, "GridColumnIndex": 0 }, { "Label": "Pos23", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.195], "Focus": [17741.5], "XYStage": [ 34990.9, -132.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos24", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.46], "Focus": [17741.4], "XYStage": [ 29678.100000000002, -958.1 ] }, "GridColumnIndex": 0 }, { "Label": "Pos25", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.44], "Focus": [17741.5], "XYStage": [ 28983.600000000002, -939.4000000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos26", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.475], "Focus": [17741.5], "XYStage": [ 23019.7, -122 ] }, "GridColumnIndex": 0 }, { "Label": "Pos27", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.44], "Focus": [17741.7], "XYStage": [ 22428, -116.80000000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos28", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.46], "Focus": [17741.7], "XYStage": [ 16364.900000000001, -181.10000000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos29", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.48000000000002], "Focus": [17741.5], "XYStage": [ 15712.1, -180 ] }, "GridColumnIndex": 0 }, { "Label": "Pos30", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.45000000000002], "Focus": [17741.7], "XYStage": [ 10346.1, 77.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos31", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.49], "Focus": [17741.4], "XYStage": [ 9755.5, 85.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos32", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.475], "Focus": [17741.5], "XYStage": [ 4775.5, 293.90000000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos33", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.445], "Focus": [17741.4], "XYStage": [ 3954.9, 293.90000000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos34", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.455], "Focus": [17741.4], "XYStage": [ -1324.9, 545.6 ] }, "GridColumnIndex": 0 }, { "Label": "Pos35", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.445], "Focus": [17741.3], "XYStage": [ -2097, 576.6 ] }, "GridColumnIndex": 0 } ], "z-step_um": 10, "GridRow": 0, "BitDepth": 16, "Depth": 2, "Channels": 6, "Source": "Micro-Manager", "ChColors": [ -16711681, -256, -65281, -1, -1, -16776961 ], "MetadataVersion": 10, "KeepShutterOpenChannels": true, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536, 65536 ], "CustomIntervals_ms": [], "Height": 1200, "MicroManagerVersion": "1.4.23 20180219", "GridColumn": 0, "UUID": "67598d21-5db5-4842-ab60-f506f7b42c7c", "PixelType": "GRAY16", "Width": 1200, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\Yan\\20191218-YC113-S7S8-miseq", "Frames": 1, "Comment": "", "UserName": "Nikon", "PixelSize_um": 1.1, "ROI": [ 0, 0, 1200, 1200 ], "Date": "2019-12-18", "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC", "P-DAPI-high" ], "KeepShutterOpenSlices": true, "IJType": 1, "ComputerName": "ZadorConfocal2", "Positions": 36, "PositionIndex": 29, "CameraTimeout": 20000, "TimeFirst": false }, "640 AO-Name": "AnalogIO", "Vincent-D3-Command": "Close" }, "FrameKey-0-4-10": { "COM4-StopBits": "1", "528 AO-MaxVolts": "5.0000", "XLIGHT Excitation Wheel-State": "1", "470 Shutter-Name": "AnalogIO", "Core-Shutter": "Vincent-D3", "555 AO-Demo": "No", "XLIGHT Dichroic-State": "2", "XYStage-StepSize-Y": "0.1000", "ZPositionUm": 268.48, "XYStage-StepSize-X": "0.1000", "PRIME-FirmwareVersion": "20.28", "PRIME-ClearCycles": "2", "520-Name": "DA Shutter", "405 Shutter-Name": "AnalogIO", "PIEZO-StepSize": "0.0050", "PRIME-ClearMode": "Pre-Exposure", "470 AO-MinVolts": "0.0000", "405 AO-SupportsTriggering": "1", "CameraChannelIndex": 0, "XYStage-StartSpeed": "500.0000", "COM9-StopBits": "2", "405 AO-Description": "NI DAC", "COM4-DelayBetweenCharsMs": "0.0000", "445 AO-Block voltage": "false", "520 AO-TriggerInputLine": "/Dev1/PFI0", "555 AO-Description": "NI DAC", "520 AO-TriggerSampleRate": "100000", "COM4-Verbose": "1", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM4-Name": "COM4", "405 Shutter-Volts": "0.0000", "Vincent-D3-Port": "COM1", "PRIME-PP 4 MIN ADU AFFECTED": "200", "470 Shutter-MinVolts": "0.0000", "PRIME-PP 3 ENABLED": "Yes", "470 AO-Description": "NI DAC", "PRIME-Description": "PVCAM API device adapter", "520 Shutter-MaxVolts": "5.0000", "520 Shutter-Volts": "0.0000", "528 AO-Sequenceable": "1", "PRIME-Binning": "1x1", "555 Shutter-MaxVolts": "5.0000", "528 Shutter-TriggerSequenceLength": "1000", "405 Shutter-Block voltage": "false", "XLIGHT Spin motor-State": "1", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "Vincent-D3-Time to open (ms)": "35.0000", "Vincent-D3-Time to close (ms)": "35.0000", "445 AO-TriggerSampleRate": "100000", "640 Shutter-IOChannel": "Dev1/ao14", "555 AO-Sequenceable": "1", "520 AO-Name": "AnalogIO", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "470 AO-Block voltage": "false", "COM1-Verbose": "1", "COM9-AnswerTimeout": "10000.0000", "528 Shutter-Name": "AnalogIO", "PRIME-Trigger Timeout (secs)": "10", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "NextFrame": 0, "470 AO-TriggerSequenceLength": "1000", "PRIME-TransposeXY": "0", "445 AO-TriggerSequenceLength": "1000", "COM1-Name": "COM1", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "COM9-DataBits": "8", "COM1-AnswerTimeout": "500.0000", "Camera": "", "TE2000-Version": "V2.50-V2.50-V2.10", "528 Shutter-Volts": "0.0000", "XLIGHT Hub-Name": "XLIGHT Hub", "PIEZO-Description": "Ludl stage driver adapter", "PositionName": "Pos29", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSampleRate": "100000", "PRIME-FanSpeedSetpoint": "High", "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "405 AO-TriggerSampleRate": "100000", "470 Shutter-Block voltage": "false", "470-State": "0", "COM3-Parity": "None", "405 AO-Volts": "0.0000", "PRIME-TransposeMirrorY": "0", "PRIME-ChipName": "GS144BSI", "470 AO-Volts": "0.0000", "PRIME-TransposeMirrorX": "0", "445 Shutter-SupportsTriggering": "0", "470 Shutter-SupportsTriggering": "0", "XLIGHT Hub-Description": "XLIGHT hub", "PRIME-PreampOffLimit": "10000", "470 AO-Sequenceable": "1", "528 Shutter-MaxVolts": "5.0000", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "Core-XYStage": "XYStage", "470 AO-TriggerSampleRate": "100000", "PRIME-Timing-PreTriggerDelayNs": "24672000.0000", "528-DA Device": "528 Shutter", "Focus-Description": "Nikon TE2000 focus adapter", "FilterBlock-State": "5", "520 AO-Demo": "No", "COM3-DelayBetweenCharsMs": "0.0000", "470 Shutter-Volts": "0.0000", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "PRIME-PP 2 THRESHOLD": "125", "PRIME-PP 4 THRESHOLD": "75", "XYStage-Acceleration": "4.0000", "470 Shutter-Demo": "No", "PRIME-Name": "PMPCIECam00", "640-DA Device": "640 Shutter", "405 AO-Block voltage": "false", "ElapsedTime-ms": 1221028, "PRIME-Offset": "100", "445 AO-IOChannel": "Dev1/ao1", "640 AO-MaxVolts": "5.0000", "PositionIndex": 29, "PRIME-PP 1 ENABLED": "Yes", "COM1-BaudRate": "9600", "640-State": "0", "COM3-Verbose": "1", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "LudlController-TransmissionDelay": "0", "PRIME-FullWellCapacity": "62000", "PRIME-SMARTStreamingEnabled": "No", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "640 Shutter-Description": "NI DAC", "528-Description": "DA device that is used as a shutter", "COM4-Description": "Serial port driver (boost:asio)", "405 Shutter-Description": "NI DAC", "TE2000-Name": "TE2000", "528 Shutter-Description": "NI DAC", "COM9-Description": "Serial port driver (boost:asio)", "LudlController-Name": "LudlController", "445 Shutter-TriggerSequenceLength": "1000", "PRIME-SerialNumber": "A18B203008", "445 AO-Volts": "0.0000", "PRIME-X-dimension": "1200", "PRIME-ShutterCloseDelay": "0", "XLIGHT Disk slider-State": "2", "Position": "Default", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 AO-IOChannel": "Dev1/ao5", "520 AO-IOChannel": "Dev1/ao4", "528 AO-TriggerSequenceLength": "1000", "405 Shutter-MaxVolts": "5.0000", "470-DA Device": "470 Shutter", "PRIME-FTCapable": "No", "Core-ChannelGroup": "Channels", "555 AO-SupportsTriggering": "0", "PRIME-Y-dimension": "1200", "Frame": 0, "555 Shutter-Sequenceable": "1", "PRIME-Timing-ExposureTimeNs": "499998000.0000", "Core-ImageProcessor": "", "528-State": "0", "520 AO-Block voltage": "false", "XLIGHT Excitation Wheel-Label": "Excitation-2", "640 Shutter-Demo": "No", "COM3-StopBits": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "520 Shutter-IOChannel": "Dev1/ao11", "Width": 1200, "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-SupportsTriggering": "0", "640 AO-TriggerSampleRate": "100000", "520-Description": "DA device that is used as a shutter", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "405 Shutter-Sequenceable": "1", "405 Shutter-SupportsTriggering": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-TriggerSampleRate": "100000", "640 Shutter-MaxVolts": "5.0000", "555 AO-MaxVolts": "5.0000", "520 AO-TriggerSequenceLength": "1000", "COM3-BaudRate": "9600", "COM9-BaudRate": "9600", "Vincent-D3-Channel #": "Ch. #1", "PRIME-PMode": "Normal", "OpticalPath-Label": "Left(100)", "BitDepth": 16, "555 AO-TriggerSequenceLength": "1000", "445 Shutter-Description": "NI DAC", "405 AO-Sequenceable": "1", "PRIME-AcquisitionMethod": "Callbacks", "555 AO-Name": "AnalogIO", "PRIME-ShutterMode": "Pre-Exposure", "COM3-Handshaking": "Off", "445 AO-SupportsTriggering": "0", "ROI": "0-0-1200-1200", "COM9-Verbose": "1", "PRIME-Color": "OFF", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "PRIME-ShutterOpenDelay": "0", "COM1-Parity": "None", "Slice": 10, "SlicePosition": 50, "COM9-DelayBetweenCharsMs": "0.0000", "555-Name": "DA Shutter", "PIEZO-LudlSingleAxisName": "B", "XLIGHT Emission Wheel-Label": "Empty", "640 Shutter-Name": "AnalogIO", "640 AO-Sequenceable": "1", "520 Shutter-Demo": "No", "528 Shutter-Demo": "No", "470-Name": "DA Shutter", "520-DA Device": "520 Shutter", "XYStage-Description": "Ludl XY stage driver adapter", "445 Shutter-MinVolts": "0.0000", "528 AO-TriggerSampleRate": "100000", "405 AO-MinVolts": "0.0000", "405 Shutter-TriggerSequenceLength": "1000", "Height": 1200, "555 AO-Block voltage": "false", "640 Shutter-Volts": "0.0000", "PixelType": "GRAY16", "445 Shutter-Block voltage": "false", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "520 Shutter-TriggerSequenceLength": "1000", "COM9-Name": "COM9", "Core-SLM": "", "555 AO-Volts": "0.0000", "555 Shutter-Block voltage": "false", "XYStage-Speed": "1800.0000", "520 AO-MaxVolts": "5.0000", "528 Shutter-SupportsTriggering": "0", "COM1-Description": "Serial port driver (boost:asio)", "PRIME-MetadataEnabled": "No", "520 Shutter-MinVolts": "0.0000", "470 Shutter-Sequenceable": "1", "PRIME-PreampDelay": "0", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-MinVolts": "0.0000", "ChannelIndex": 4, "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Core-AutoShutter": "1", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "Exposure-ms": 20, "PRIME-PP 2 ENABLED": "Yes", "640 Shutter-TriggerSampleRate": "100000", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "470 Shutter-IOChannel": "Dev1/ao10", "405 AO-Demo": "No", "PixelSizeUm": 1.1, "470 AO-Demo": "No", "470 Shutter-MaxVolts": "5.0000", "Vincent-D3-Name": "Vincent-D3", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FileName": "img_000000000_P-DIC_010.tif", "LudlController-Reset": "Operate", "PRIME-Exposure": "20.00", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "445-Name": "DA Shutter", "555 Shutter-Name": "AnalogIO", "520 Shutter-TriggerSampleRate": "100000", "445 Shutter-IOChannel": "Dev1/ao9", "PRIME-TransposeCorrection": "0", "XYStage-StepSize": "0.1000", "445-Description": "DA device that is used as a shutter", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 AO-IOChannel": "Dev1/ao7", "520 Shutter-Name": "AnalogIO", "COM3-DataBits": "8", "640 AO-Volts": "0.0000", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "555 Shutter-IOChannel": "Dev1/ao13", "WaitInterval": null, "520 Shutter-Sequenceable": "1", "YPositionUm": -180, "445-State": "0", "445-DA Device": "445 Shutter", "PRIME-CircularBufferAutoSize": "ON", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "555 AO-TriggerSampleRate": "100000", "445 Shutter-Volts": "0.0000", "XLIGHT Hub-Port": "COM3", "Core-AutoFocus": "", "COM4-Handshaking": "Off", "528 Shutter-MinVolts": "0.0000", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "445 Shutter-Name": "AnalogIO", "528 Shutter-Sequenceable": "1", "XYStage-TransposeMirrorX": "0", "XYStage-TransposeMirrorY": "0", "PRIME-ReadoutRate": "100MHz 16bit", "XYStage-Name": "Stage", "640 AO-SupportsTriggering": "0", "640-Name": "DA Shutter", "640 Shutter-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "640 AO-Block voltage": "false", "445 Shutter-Sequenceable": "1", "470 AO-MaxVolts": "5.0000", "COM4-BaudRate": "9600", "470-Description": "DA device that is used as a shutter", "520 Shutter-Description": "NI DAC", "PRIME-CCDTemperature": "-14.8500", "Time": "2019-12-18 23:11:21 -0500", "640 AO-TriggerSequenceLength": "1000", "470 AO-Name": "AnalogIO", "XLIGHT Disk slider-Label": "Disk Pos 40um", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "Binning": "1x1", "TE2000-ModelType": "1", "COM3-AnswerTimeout": "500.0000", "555 Shutter-SupportsTriggering": "0", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "TE2000-Port": "COM4", "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Description": "NI DAC", "COM4-AnswerTimeout": "500.0000", "COM1-DelayBetweenCharsMs": "0.0000", "FilterBlock-Label": "Confocal", "445 AO-Name": "AnalogIO", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-Timing-ReadoutTimeNs": "24672000.0000", "528 AO-Demo": "No", "520 AO-Sequenceable": "1", "555-Description": "DA device that is used as a shutter", "405-DA Device": "405 Shutter", "405 AO-TriggerSequenceLength": "1000", "640 AO-MinVolts": "0.0000", "640 Shutter-Block voltage": "false", "FrameIndex": 0, "PRIME-Port": "CMOS", "555 AO-MinVolts": "0.0000", "COM3-Description": "Serial port driver (boost:asio)", "528 AO-Description": "NI DAC", "555 AO-IOChannel": "Dev1/ao6", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "555 AO-TriggerInputLine": "/Dev1/PFI0", "PRIME-ActualInterval-ms": "390.5200", "LudlController-Port": "COM9", "445 Shutter-Demo": "No", "PRIME-Trigger-Expose Out-Mux": "1", "SliceIndex": 10, "PRIME-CCDTemperatureSetPoint": "-15.0000", "405 Shutter-MinVolts": "0.0000", "PRIME-ExposeOutMode": "First Row", "AxisPositions": null, "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Volts": "0.0000", "528-Name": "DA Shutter", "555-State": "0", "XLIGHT Dichroic-Label": "Di03-R635-t1", "445 AO-Sequenceable": "1", "405-Name": "DA Shutter", "405 AO-IOChannel": "Dev1/ao0", "Core-Camera": "PRIME", "Focus-Name": "Focus", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "405 Shutter-IOChannel": "Dev1/ao8", "640 AO-Demo": "No", "640 Shutter-SupportsTriggering": "0", "445 AO-Demo": "No", "555-DA Device": "555 Shutter", "470 Shutter-Description": "NI DAC", "528 AO-Volts": "0.0000", "COM1-DataBits": "8", "Vincent-D3-Address": "x", "520 AO-SupportsTriggering": "0", "405-Description": "DA device that is used as a shutter", "640 Shutter-MinVolts": "0.0000", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "405 AO-Name": "AnalogIO", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "640 AO-TriggerInputLine": "/Dev1/PFI0", "405 AO-TriggerInputLine": "/Dev1/PFI0", "640-Description": "DA device that is used as a shutter", "445 Shutter-TriggerSampleRate": "100000", "470 Shutter-TriggerSequenceLength": "1000", "COM1-StopBits": "1", "520 Shutter-SupportsTriggering": "0", "405-State": "0", "Core-Initialize": "1", "XLIGHT Emission Wheel-State": "3", "520 AO-Description": "NI DAC", "528 AO-TriggerInputLine": "/Dev1/PFI0", "445 Shutter-MaxVolts": "5.0000", "COM9-Handshaking": "Off", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "XLIGHT Spin motor-Label": "On (spinning)", "PRIME-PP 5 ENABLED": "No", "UUID": "cc741b95-92c0-4537-962f-2ea65eead63a", "PRIME-TriggerMode": "Internal Trigger", "COM1-Handshaking": "Off", "PRIME-Timing-PostTriggerDelayNs": "49344000.0000", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "Focus-Position": "17741.5000", "528 Shutter-Block voltage": "false", "Channel": "P-DIC", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "555 Shutter-Demo": "No", "405 Shutter-Demo": "No", "528 AO-Name": "AnalogIO", "520-State": "0", "Core-Galvo": "", "405 Shutter-TriggerSampleRate": "100000", "Focus-FocusStepSize_(nm)": "100", "PRIME-PixelType": "16bit", "COM4-DataBits": "8", "470 AO-IOChannel": "Dev1/ao2", "520 AO-MinVolts": "0.0000", "COM9-Parity": "None", "PRIME-CircularBufferEnabled": "ON", "PRIME-PP 6 Reset": "No", "640 AO-Description": "NI DAC", "Source": "PRIME", "445 AO-MinVolts": "0.0000", "PIEZO-Name": "Stage", "445 AO-Description": "NI DAC", "PRIME-CircularBufferFrameCount": "46", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "520 AO-Volts": "0.0000", "528 Shutter-TriggerSampleRate": "100000", "XPositionUm": 15712.1, "OpticalPath-State": "4", "PRIME-PP 3 THRESHOLD": "80", "PRIME-PVCAM Version": "3.7.1", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Gain": "1-HDR", "640 Shutter-TriggerSequenceLength": "1000", "528 AO-SupportsTriggering": "0", "445 AO-TriggerInputLine": "/Dev1/PFI0", "OpticalPath-Name": "OpticalPath", "PRIME-PP 4 ENABLED": "Yes", "PRIME-PVCAM Adapter Version": "1.3.29", "COM3-Name": "COM3", "405 AO-MaxVolts": "5.0000", "520 Shutter-Block voltage": "false", "PRIME-Timing-ClearingTimeNs": "49344000.0000", "Core-Focus": "PIEZO", "528 AO-Block voltage": "false", "TE2000-Description": "Nikon TE2000 hub", "528 AO-MinVolts": "0.0000", "COM4-Parity": "None", "Summary": { "Slices": 11, "ChContrastMin": [ 0, 0, 0, 0, 0, 0 ], "PixelAspect": 1, "SlicesFirst": true, "Interval_ms": 0, "Time": "2019-12-18 23:10:50 -0500", "Prefix": "seq_5", "InitialPositionList": [ { "Label": "Pos0", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.21], "Focus": [17741.5], "XYStage": [ 44542.200000000004, 31636.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos1", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.22], "Focus": [17741.4], "XYStage": [ 43801.100000000006, 31637.9 ] }, "GridColumnIndex": 0 }, { "Label": "Pos2", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.5], "XYStage": [ 38561.3, 32040.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos3", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.185], "Focus": [17741.3], "XYStage": [ 37698.4, 32041.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos4", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.16], "Focus": [17741.5], "XYStage": [ 32375.4, 31758.9 ] }, "GridColumnIndex": 0 }, { "Label": "Pos5", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.17000000000002], "Focus": [17741.3], "XYStage": [ 31770.7, 31758.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos6", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.4], "XYStage": [ 26536.800000000003, 31068.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos7", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.205], "Focus": [17741.4], "XYStage": [ 25420.2, 31068.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos8", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.85], "Focus": [17741.3], "XYStage": [ 21059, 31508 ] }, "GridColumnIndex": 0 }, { "Label": "Pos9", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.82], "Focus": [17741.3], "XYStage": [ 19731.9, 31506.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos10", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.79], "Focus": [17741.4], "XYStage": [ 14528.5, 32007.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos11", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.83], "Focus": [17741.2], "XYStage": [ 13830.300000000001, 32009.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos12", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.79], "Focus": [17741.3], "XYStage": [ 8775.300000000001, 32017.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos13", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.815], "Focus": [17741.3], "XYStage": [ 8109.200000000001, 32008.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos14", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.805], "Focus": [17741.3], "XYStage": [ 3483.3, 31908.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos15", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.82], "Focus": [17741.2], "XYStage": [ 2686.8, 31903.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos16", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [211.48000000000002], "Focus": [17741.1], "XYStage": [ -1978.9, 31973.600000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos17", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [211.51500000000001], "Focus": [17741.1], "XYStage": [ -2957.8, 31982.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos18", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.195], "Focus": [17741.5], "XYStage": [ 47747.4, -576.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos19", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.23000000000002], "Focus": [17741.6], "XYStage": [ 47053.9, -576.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos20", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.205], "Focus": [17741.4], "XYStage": [ 41871.200000000004, -479.8 ] }, "GridColumnIndex": 0 }, { "Label": "Pos21", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.4], "XYStage": [ 40882.600000000006, -392 ] }, "GridColumnIndex": 0 }, { "Label": "Pos22", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.24], "Focus": [17741.4], "XYStage": [ 35905.6, -135.3 ] }, "GridColumnIndex": 0 }, { "Label": "Pos23", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.195], "Focus": [17741.5], "XYStage": [ 34990.9, -132.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos24", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.46], "Focus": [17741.4], "XYStage": [ 29678.100000000002, -958.1 ] }, "GridColumnIndex": 0 }, { "Label": "Pos25", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.44], "Focus": [17741.5], "XYStage": [ 28983.600000000002, -939.4000000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos26", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.475], "Focus": [17741.5], "XYStage": [ 23019.7, -122 ] }, "GridColumnIndex": 0 }, { "Label": "Pos27", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.44], "Focus": [17741.7], "XYStage": [ 22428, -116.80000000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos28", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.46], "Focus": [17741.7], "XYStage": [ 16364.900000000001, -181.10000000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos29", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.48000000000002], "Focus": [17741.5], "XYStage": [ 15712.1, -180 ] }, "GridColumnIndex": 0 }, { "Label": "Pos30", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.45000000000002], "Focus": [17741.7], "XYStage": [ 10346.1, 77.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos31", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.49], "Focus": [17741.4], "XYStage": [ 9755.5, 85.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos32", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.475], "Focus": [17741.5], "XYStage": [ 4775.5, 293.90000000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos33", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.445], "Focus": [17741.4], "XYStage": [ 3954.9, 293.90000000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos34", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.455], "Focus": [17741.4], "XYStage": [ -1324.9, 545.6 ] }, "GridColumnIndex": 0 }, { "Label": "Pos35", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.445], "Focus": [17741.3], "XYStage": [ -2097, 576.6 ] }, "GridColumnIndex": 0 } ], "z-step_um": 10, "GridRow": 0, "BitDepth": 16, "Depth": 2, "Channels": 6, "Source": "Micro-Manager", "ChColors": [ -16711681, -256, -65281, -1, -1, -16776961 ], "MetadataVersion": 10, "KeepShutterOpenChannels": true, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536, 65536 ], "CustomIntervals_ms": [], "Height": 1200, "MicroManagerVersion": "1.4.23 20180219", "GridColumn": 0, "UUID": "67598d21-5db5-4842-ab60-f506f7b42c7c", "PixelType": "GRAY16", "Width": 1200, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\Yan\\20191218-YC113-S7S8-miseq", "Frames": 1, "Comment": "", "UserName": "Nikon", "PixelSize_um": 1.1, "ROI": [ 0, 0, 1200, 1200 ], "Date": "2019-12-18", "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC", "P-DAPI-high" ], "KeepShutterOpenSlices": true, "IJType": 1, "ComputerName": "ZadorConfocal2", "Positions": 36, "PositionIndex": 29, "CameraTimeout": 20000, "TimeFirst": false }, "640 AO-Name": "AnalogIO", "Vincent-D3-Command": "Close" }, "FrameKey-0-5-0": { "COM4-StopBits": "1", "528 AO-MaxVolts": "5.0000", "XLIGHT Excitation Wheel-State": "1", "470 Shutter-Name": "AnalogIO", "Core-Shutter": "405", "555 AO-Demo": "No", "XLIGHT Dichroic-State": "2", "XYStage-StepSize-Y": "0.1000", "ZPositionUm": 168.48000000000002, "XYStage-StepSize-X": "0.1000", "PRIME-FirmwareVersion": "20.28", "PRIME-ClearCycles": "2", "520-Name": "DA Shutter", "405 Shutter-Name": "AnalogIO", "PIEZO-StepSize": "0.0050", "PRIME-ClearMode": "Pre-Exposure", "470 AO-MinVolts": "0.0000", "405 AO-SupportsTriggering": "1", "CameraChannelIndex": 0, "XYStage-StartSpeed": "500.0000", "COM9-StopBits": "2", "405 AO-Description": "NI DAC", "COM4-DelayBetweenCharsMs": "0.0000", "445 AO-Block voltage": "false", "520 AO-TriggerInputLine": "/Dev1/PFI0", "555 AO-Description": "NI DAC", "520 AO-TriggerSampleRate": "100000", "COM4-Verbose": "1", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM4-Name": "COM4", "405 Shutter-Volts": "5.0000", "Vincent-D3-Port": "COM1", "PRIME-PP 4 MIN ADU AFFECTED": "200", "470 Shutter-MinVolts": "0.0000", "PRIME-PP 3 ENABLED": "Yes", "470 AO-Description": "NI DAC", "PRIME-Description": "PVCAM API device adapter", "520 Shutter-MaxVolts": "5.0000", "520 Shutter-Volts": "0.0000", "528 AO-Sequenceable": "1", "PRIME-Binning": "1x1", "555 Shutter-MaxVolts": "5.0000", "528 Shutter-TriggerSequenceLength": "1000", "405 Shutter-Block voltage": "false", "XLIGHT Spin motor-State": "1", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "Vincent-D3-Time to open (ms)": "35.0000", "Vincent-D3-Time to close (ms)": "35.0000", "445 AO-TriggerSampleRate": "100000", "640 Shutter-IOChannel": "Dev1/ao14", "555 AO-Sequenceable": "1", "520 AO-Name": "AnalogIO", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "470 AO-Block voltage": "false", "COM1-Verbose": "1", "COM9-AnswerTimeout": "10000.0000", "528 Shutter-Name": "AnalogIO", "PRIME-Trigger Timeout (secs)": "10", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "NextFrame": 0, "470 AO-TriggerSequenceLength": "1000", "PRIME-TransposeXY": "0", "445 AO-TriggerSequenceLength": "1000", "COM1-Name": "COM1", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "COM9-DataBits": "8", "COM1-AnswerTimeout": "500.0000", "Camera": "", "TE2000-Version": "V2.50-V2.50-V2.10", "528 Shutter-Volts": "0.0000", "XLIGHT Hub-Name": "XLIGHT Hub", "PIEZO-Description": "Ludl stage driver adapter", "PositionName": "Pos29", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSampleRate": "100000", "PRIME-FanSpeedSetpoint": "High", "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "405 AO-TriggerSampleRate": "100000", "470 Shutter-Block voltage": "false", "470-State": "0", "COM3-Parity": "None", "405 AO-Volts": "4.5000", "PRIME-TransposeMirrorY": "0", "PRIME-ChipName": "GS144BSI", "470 AO-Volts": "0.0000", "PRIME-TransposeMirrorX": "0", "445 Shutter-SupportsTriggering": "0", "470 Shutter-SupportsTriggering": "0", "XLIGHT Hub-Description": "XLIGHT hub", "PRIME-PreampOffLimit": "10000", "470 AO-Sequenceable": "1", "528 Shutter-MaxVolts": "5.0000", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "Core-XYStage": "XYStage", "470 AO-TriggerSampleRate": "100000", "PRIME-Timing-PreTriggerDelayNs": "24672000.0000", "528-DA Device": "528 Shutter", "Focus-Description": "Nikon TE2000 focus adapter", "FilterBlock-State": "5", "520 AO-Demo": "No", "COM3-DelayBetweenCharsMs": "0.0000", "470 Shutter-Volts": "0.0000", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "PRIME-PP 2 THRESHOLD": "125", "PRIME-PP 4 THRESHOLD": "75", "XYStage-Acceleration": "4.0000", "470 Shutter-Demo": "No", "PRIME-Name": "PMPCIECam00", "640-DA Device": "640 Shutter", "405 AO-Block voltage": "false", "ElapsedTime-ms": 1222404, "PRIME-Offset": "100", "445 AO-IOChannel": "Dev1/ao1", "640 AO-MaxVolts": "5.0000", "PositionIndex": 29, "PRIME-PP 1 ENABLED": "Yes", "COM1-BaudRate": "9600", "640-State": "0", "COM3-Verbose": "1", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "LudlController-TransmissionDelay": "0", "PRIME-FullWellCapacity": "62000", "PRIME-SMARTStreamingEnabled": "No", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "640 Shutter-Description": "NI DAC", "528-Description": "DA device that is used as a shutter", "COM4-Description": "Serial port driver (boost:asio)", "405 Shutter-Description": "NI DAC", "TE2000-Name": "TE2000", "528 Shutter-Description": "NI DAC", "COM9-Description": "Serial port driver (boost:asio)", "LudlController-Name": "LudlController", "445 Shutter-TriggerSequenceLength": "1000", "PRIME-SerialNumber": "A18B203008", "445 AO-Volts": "0.0000", "PRIME-X-dimension": "1200", "PRIME-ShutterCloseDelay": "0", "XLIGHT Disk slider-State": "2", "Position": "Default", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 AO-IOChannel": "Dev1/ao5", "520 AO-IOChannel": "Dev1/ao4", "528 AO-TriggerSequenceLength": "1000", "405 Shutter-MaxVolts": "5.0000", "470-DA Device": "470 Shutter", "PRIME-FTCapable": "No", "Core-ChannelGroup": "Channels", "555 AO-SupportsTriggering": "0", "PRIME-Y-dimension": "1200", "Frame": 0, "555 Shutter-Sequenceable": "1", "PRIME-Timing-ExposureTimeNs": "499998000.0000", "Core-ImageProcessor": "", "528-State": "0", "520 AO-Block voltage": "false", "XLIGHT Excitation Wheel-Label": "QUAD", "640 Shutter-Demo": "No", "COM3-StopBits": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "520 Shutter-IOChannel": "Dev1/ao11", "Width": 1200, "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-SupportsTriggering": "0", "640 AO-TriggerSampleRate": "100000", "520-Description": "DA device that is used as a shutter", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "405 Shutter-Sequenceable": "1", "405 Shutter-SupportsTriggering": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-TriggerSampleRate": "100000", "640 Shutter-MaxVolts": "5.0000", "555 AO-MaxVolts": "5.0000", "520 AO-TriggerSequenceLength": "1000", "COM3-BaudRate": "9600", "COM9-BaudRate": "9600", "Vincent-D3-Channel #": "Ch. #1", "PRIME-PMode": "Normal", "OpticalPath-Label": "Left(100)", "BitDepth": 16, "555 AO-TriggerSequenceLength": "1000", "445 Shutter-Description": "NI DAC", "405 AO-Sequenceable": "1", "PRIME-AcquisitionMethod": "Callbacks", "555 AO-Name": "AnalogIO", "PRIME-ShutterMode": "Pre-Exposure", "COM3-Handshaking": "Off", "445 AO-SupportsTriggering": "0", "ROI": "0-0-1200-1200", "COM9-Verbose": "1", "PRIME-Color": "OFF", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "PRIME-ShutterOpenDelay": "0", "COM1-Parity": "None", "Slice": 0, "SlicePosition": -50, "COM9-DelayBetweenCharsMs": "0.0000", "555-Name": "DA Shutter", "PIEZO-LudlSingleAxisName": "B", "XLIGHT Emission Wheel-Label": "QUAD", "640 Shutter-Name": "AnalogIO", "640 AO-Sequenceable": "1", "520 Shutter-Demo": "No", "528 Shutter-Demo": "No", "470-Name": "DA Shutter", "520-DA Device": "520 Shutter", "XYStage-Description": "Ludl XY stage driver adapter", "445 Shutter-MinVolts": "0.0000", "528 AO-TriggerSampleRate": "100000", "405 AO-MinVolts": "0.0000", "405 Shutter-TriggerSequenceLength": "1000", "Height": 1200, "555 AO-Block voltage": "false", "640 Shutter-Volts": "0.0000", "PixelType": "GRAY16", "445 Shutter-Block voltage": "false", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "520 Shutter-TriggerSequenceLength": "1000", "COM9-Name": "COM9", "Core-SLM": "", "555 AO-Volts": "0.0000", "555 Shutter-Block voltage": "false", "XYStage-Speed": "1800.0000", "520 AO-MaxVolts": "5.0000", "528 Shutter-SupportsTriggering": "0", "COM1-Description": "Serial port driver (boost:asio)", "PRIME-MetadataEnabled": "No", "520 Shutter-MinVolts": "0.0000", "470 Shutter-Sequenceable": "1", "PRIME-PreampDelay": "0", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-MinVolts": "0.0000", "ChannelIndex": 5, "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Core-AutoShutter": "1", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "Exposure-ms": 300, "PRIME-PP 2 ENABLED": "Yes", "640 Shutter-TriggerSampleRate": "100000", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "470 Shutter-IOChannel": "Dev1/ao10", "405 AO-Demo": "No", "PixelSizeUm": 1.1, "470 AO-Demo": "No", "470 Shutter-MaxVolts": "5.0000", "Vincent-D3-Name": "Vincent-D3", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FileName": "img_000000000_P-DAPI-high_000.tif", "LudlController-Reset": "Operate", "PRIME-Exposure": "300.00", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "445-Name": "DA Shutter", "555 Shutter-Name": "AnalogIO", "520 Shutter-TriggerSampleRate": "100000", "445 Shutter-IOChannel": "Dev1/ao9", "PRIME-TransposeCorrection": "0", "XYStage-StepSize": "0.1000", "445-Description": "DA device that is used as a shutter", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 AO-IOChannel": "Dev1/ao7", "520 Shutter-Name": "AnalogIO", "COM3-DataBits": "8", "640 AO-Volts": "0.0000", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "555 Shutter-IOChannel": "Dev1/ao13", "WaitInterval": null, "520 Shutter-Sequenceable": "1", "YPositionUm": -180, "445-State": "0", "445-DA Device": "445 Shutter", "PRIME-CircularBufferAutoSize": "ON", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "555 AO-TriggerSampleRate": "100000", "445 Shutter-Volts": "0.0000", "XLIGHT Hub-Port": "COM3", "Core-AutoFocus": "", "COM4-Handshaking": "Off", "528 Shutter-MinVolts": "0.0000", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "445 Shutter-Name": "AnalogIO", "528 Shutter-Sequenceable": "1", "XYStage-TransposeMirrorX": "0", "XYStage-TransposeMirrorY": "0", "PRIME-ReadoutRate": "100MHz 16bit", "XYStage-Name": "Stage", "640 AO-SupportsTriggering": "0", "640-Name": "DA Shutter", "640 Shutter-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "640 AO-Block voltage": "false", "445 Shutter-Sequenceable": "1", "470 AO-MaxVolts": "5.0000", "COM4-BaudRate": "9600", "470-Description": "DA device that is used as a shutter", "520 Shutter-Description": "NI DAC", "PRIME-CCDTemperature": "-14.8500", "Time": "2019-12-18 23:11:22 -0500", "640 AO-TriggerSequenceLength": "1000", "470 AO-Name": "AnalogIO", "XLIGHT Disk slider-Label": "Disk Pos 40um", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "Binning": "1x1", "TE2000-ModelType": "1", "COM3-AnswerTimeout": "500.0000", "555 Shutter-SupportsTriggering": "0", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "TE2000-Port": "COM4", "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Description": "NI DAC", "COM4-AnswerTimeout": "500.0000", "COM1-DelayBetweenCharsMs": "0.0000", "FilterBlock-Label": "Confocal", "445 AO-Name": "AnalogIO", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-Timing-ReadoutTimeNs": "24672000.0000", "528 AO-Demo": "No", "520 AO-Sequenceable": "1", "555-Description": "DA device that is used as a shutter", "405-DA Device": "405 Shutter", "405 AO-TriggerSequenceLength": "1000", "640 AO-MinVolts": "0.0000", "640 Shutter-Block voltage": "false", "FrameIndex": 0, "PRIME-Port": "CMOS", "555 AO-MinVolts": "0.0000", "COM3-Description": "Serial port driver (boost:asio)", "528 AO-Description": "NI DAC", "555 AO-IOChannel": "Dev1/ao6", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "555 AO-TriggerInputLine": "/Dev1/PFI0", "PRIME-ActualInterval-ms": "390.5200", "LudlController-Port": "COM9", "445 Shutter-Demo": "No", "PRIME-Trigger-Expose Out-Mux": "1", "SliceIndex": 0, "PRIME-CCDTemperatureSetPoint": "-15.0000", "405 Shutter-MinVolts": "0.0000", "PRIME-ExposeOutMode": "First Row", "AxisPositions": null, "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Volts": "0.0000", "528-Name": "DA Shutter", "555-State": "0", "XLIGHT Dichroic-Label": "QUAD", "445 AO-Sequenceable": "1", "405-Name": "DA Shutter", "405 AO-IOChannel": "Dev1/ao0", "Core-Camera": "PRIME", "Focus-Name": "Focus", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "405 Shutter-IOChannel": "Dev1/ao8", "640 AO-Demo": "No", "640 Shutter-SupportsTriggering": "0", "445 AO-Demo": "No", "555-DA Device": "555 Shutter", "470 Shutter-Description": "NI DAC", "528 AO-Volts": "0.0000", "COM1-DataBits": "8", "Vincent-D3-Address": "x", "520 AO-SupportsTriggering": "0", "405-Description": "DA device that is used as a shutter", "640 Shutter-MinVolts": "0.0000", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "405 AO-Name": "AnalogIO", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "640 AO-TriggerInputLine": "/Dev1/PFI0", "405 AO-TriggerInputLine": "/Dev1/PFI0", "640-Description": "DA device that is used as a shutter", "445 Shutter-TriggerSampleRate": "100000", "470 Shutter-TriggerSequenceLength": "1000", "COM1-StopBits": "1", "520 Shutter-SupportsTriggering": "0", "405-State": "1", "Core-Initialize": "1", "XLIGHT Emission Wheel-State": "3", "520 AO-Description": "NI DAC", "528 AO-TriggerInputLine": "/Dev1/PFI0", "445 Shutter-MaxVolts": "5.0000", "COM9-Handshaking": "Off", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "XLIGHT Spin motor-Label": "On (spinning)", "PRIME-PP 5 ENABLED": "No", "UUID": "db8aecf2-29d1-4636-9569-b724b87fc7b1", "PRIME-TriggerMode": "Internal Trigger", "COM1-Handshaking": "Off", "PRIME-Timing-PostTriggerDelayNs": "49344000.0000", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "Focus-Position": "17741.5000", "528 Shutter-Block voltage": "false", "Channel": "P-DAPI-high", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "555 Shutter-Demo": "No", "405 Shutter-Demo": "No", "528 AO-Name": "AnalogIO", "520-State": "0", "Core-Galvo": "", "405 Shutter-TriggerSampleRate": "100000", "Focus-FocusStepSize_(nm)": "100", "PRIME-PixelType": "16bit", "COM4-DataBits": "8", "470 AO-IOChannel": "Dev1/ao2", "520 AO-MinVolts": "0.0000", "COM9-Parity": "None", "PRIME-CircularBufferEnabled": "ON", "PRIME-PP 6 Reset": "No", "640 AO-Description": "NI DAC", "Source": "PRIME", "445 AO-MinVolts": "0.0000", "PIEZO-Name": "Stage", "445 AO-Description": "NI DAC", "PRIME-CircularBufferFrameCount": "46", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "520 AO-Volts": "0.0000", "528 Shutter-TriggerSampleRate": "100000", "XPositionUm": 15712.1, "OpticalPath-State": "4", "PRIME-PP 3 THRESHOLD": "80", "PRIME-PVCAM Version": "3.7.1", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Gain": "1-HDR", "640 Shutter-TriggerSequenceLength": "1000", "528 AO-SupportsTriggering": "0", "445 AO-TriggerInputLine": "/Dev1/PFI0", "OpticalPath-Name": "OpticalPath", "PRIME-PP 4 ENABLED": "Yes", "PRIME-PVCAM Adapter Version": "1.3.29", "COM3-Name": "COM3", "405 AO-MaxVolts": "5.0000", "520 Shutter-Block voltage": "false", "PRIME-Timing-ClearingTimeNs": "49344000.0000", "Core-Focus": "PIEZO", "528 AO-Block voltage": "false", "TE2000-Description": "Nikon TE2000 hub", "528 AO-MinVolts": "0.0000", "COM4-Parity": "None", "Summary": { "Slices": 11, "ChContrastMin": [ 0, 0, 0, 0, 0, 0 ], "PixelAspect": 1, "SlicesFirst": true, "Interval_ms": 0, "Time": "2019-12-18 23:10:50 -0500", "Prefix": "seq_5", "InitialPositionList": [ { "Label": "Pos0", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.21], "Focus": [17741.5], "XYStage": [ 44542.200000000004, 31636.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos1", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.22], "Focus": [17741.4], "XYStage": [ 43801.100000000006, 31637.9 ] }, "GridColumnIndex": 0 }, { "Label": "Pos2", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.5], "XYStage": [ 38561.3, 32040.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos3", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.185], "Focus": [17741.3], "XYStage": [ 37698.4, 32041.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos4", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.16], "Focus": [17741.5], "XYStage": [ 32375.4, 31758.9 ] }, "GridColumnIndex": 0 }, { "Label": "Pos5", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.17000000000002], "Focus": [17741.3], "XYStage": [ 31770.7, 31758.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos6", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.4], "XYStage": [ 26536.800000000003, 31068.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos7", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.205], "Focus": [17741.4], "XYStage": [ 25420.2, 31068.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos8", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.85], "Focus": [17741.3], "XYStage": [ 21059, 31508 ] }, "GridColumnIndex": 0 }, { "Label": "Pos9", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.82], "Focus": [17741.3], "XYStage": [ 19731.9, 31506.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos10", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.79], "Focus": [17741.4], "XYStage": [ 14528.5, 32007.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos11", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.83], "Focus": [17741.2], "XYStage": [ 13830.300000000001, 32009.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos12", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.79], "Focus": [17741.3], "XYStage": [ 8775.300000000001, 32017.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos13", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.815], "Focus": [17741.3], "XYStage": [ 8109.200000000001, 32008.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos14", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.805], "Focus": [17741.3], "XYStage": [ 3483.3, 31908.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos15", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.82], "Focus": [17741.2], "XYStage": [ 2686.8, 31903.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos16", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [211.48000000000002], "Focus": [17741.1], "XYStage": [ -1978.9, 31973.600000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos17", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [211.51500000000001], "Focus": [17741.1], "XYStage": [ -2957.8, 31982.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos18", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.195], "Focus": [17741.5], "XYStage": [ 47747.4, -576.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos19", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.23000000000002], "Focus": [17741.6], "XYStage": [ 47053.9, -576.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos20", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.205], "Focus": [17741.4], "XYStage": [ 41871.200000000004, -479.8 ] }, "GridColumnIndex": 0 }, { "Label": "Pos21", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.4], "XYStage": [ 40882.600000000006, -392 ] }, "GridColumnIndex": 0 }, { "Label": "Pos22", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.24], "Focus": [17741.4], "XYStage": [ 35905.6, -135.3 ] }, "GridColumnIndex": 0 }, { "Label": "Pos23", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.195], "Focus": [17741.5], "XYStage": [ 34990.9, -132.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos24", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.46], "Focus": [17741.4], "XYStage": [ 29678.100000000002, -958.1 ] }, "GridColumnIndex": 0 }, { "Label": "Pos25", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.44], "Focus": [17741.5], "XYStage": [ 28983.600000000002, -939.4000000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos26", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.475], "Focus": [17741.5], "XYStage": [ 23019.7, -122 ] }, "GridColumnIndex": 0 }, { "Label": "Pos27", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.44], "Focus": [17741.7], "XYStage": [ 22428, -116.80000000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos28", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.46], "Focus": [17741.7], "XYStage": [ 16364.900000000001, -181.10000000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos29", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.48000000000002], "Focus": [17741.5], "XYStage": [ 15712.1, -180 ] }, "GridColumnIndex": 0 }, { "Label": "Pos30", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.45000000000002], "Focus": [17741.7], "XYStage": [ 10346.1, 77.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos31", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.49], "Focus": [17741.4], "XYStage": [ 9755.5, 85.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos32", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.475], "Focus": [17741.5], "XYStage": [ 4775.5, 293.90000000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos33", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.445], "Focus": [17741.4], "XYStage": [ 3954.9, 293.90000000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos34", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.455], "Focus": [17741.4], "XYStage": [ -1324.9, 545.6 ] }, "GridColumnIndex": 0 }, { "Label": "Pos35", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.445], "Focus": [17741.3], "XYStage": [ -2097, 576.6 ] }, "GridColumnIndex": 0 } ], "z-step_um": 10, "GridRow": 0, "BitDepth": 16, "Depth": 2, "Channels": 6, "Source": "Micro-Manager", "ChColors": [ -16711681, -256, -65281, -1, -1, -16776961 ], "MetadataVersion": 10, "KeepShutterOpenChannels": true, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536, 65536 ], "CustomIntervals_ms": [], "Height": 1200, "MicroManagerVersion": "1.4.23 20180219", "GridColumn": 0, "UUID": "67598d21-5db5-4842-ab60-f506f7b42c7c", "PixelType": "GRAY16", "Width": 1200, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\Yan\\20191218-YC113-S7S8-miseq", "Frames": 1, "Comment": "", "UserName": "Nikon", "PixelSize_um": 1.1, "ROI": [ 0, 0, 1200, 1200 ], "Date": "2019-12-18", "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC", "P-DAPI-high" ], "KeepShutterOpenSlices": true, "IJType": 1, "ComputerName": "ZadorConfocal2", "Positions": 36, "PositionIndex": 29, "CameraTimeout": 20000, "TimeFirst": false }, "640 AO-Name": "AnalogIO", "Vincent-D3-Command": "Close" }, "FrameKey-0-5-1": { "COM4-StopBits": "1", "528 AO-MaxVolts": "5.0000", "XLIGHT Excitation Wheel-State": "1", "470 Shutter-Name": "AnalogIO", "Core-Shutter": "405", "555 AO-Demo": "No", "XLIGHT Dichroic-State": "2", "XYStage-StepSize-Y": "0.1000", "ZPositionUm": 178.48000000000002, "XYStage-StepSize-X": "0.1000", "PRIME-FirmwareVersion": "20.28", "PRIME-ClearCycles": "2", "520-Name": "DA Shutter", "405 Shutter-Name": "AnalogIO", "PIEZO-StepSize": "0.0050", "PRIME-ClearMode": "Pre-Exposure", "470 AO-MinVolts": "0.0000", "405 AO-SupportsTriggering": "1", "CameraChannelIndex": 0, "XYStage-StartSpeed": "500.0000", "COM9-StopBits": "2", "405 AO-Description": "NI DAC", "COM4-DelayBetweenCharsMs": "0.0000", "445 AO-Block voltage": "false", "520 AO-TriggerInputLine": "/Dev1/PFI0", "555 AO-Description": "NI DAC", "520 AO-TriggerSampleRate": "100000", "COM4-Verbose": "1", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM4-Name": "COM4", "405 Shutter-Volts": "5.0000", "Vincent-D3-Port": "COM1", "PRIME-PP 4 MIN ADU AFFECTED": "200", "470 Shutter-MinVolts": "0.0000", "PRIME-PP 3 ENABLED": "Yes", "470 AO-Description": "NI DAC", "PRIME-Description": "PVCAM API device adapter", "520 Shutter-MaxVolts": "5.0000", "520 Shutter-Volts": "0.0000", "528 AO-Sequenceable": "1", "PRIME-Binning": "1x1", "555 Shutter-MaxVolts": "5.0000", "528 Shutter-TriggerSequenceLength": "1000", "405 Shutter-Block voltage": "false", "XLIGHT Spin motor-State": "1", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "Vincent-D3-Time to open (ms)": "35.0000", "Vincent-D3-Time to close (ms)": "35.0000", "445 AO-TriggerSampleRate": "100000", "640 Shutter-IOChannel": "Dev1/ao14", "555 AO-Sequenceable": "1", "520 AO-Name": "AnalogIO", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "470 AO-Block voltage": "false", "COM1-Verbose": "1", "COM9-AnswerTimeout": "10000.0000", "528 Shutter-Name": "AnalogIO", "PRIME-Trigger Timeout (secs)": "10", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "NextFrame": 0, "470 AO-TriggerSequenceLength": "1000", "PRIME-TransposeXY": "0", "445 AO-TriggerSequenceLength": "1000", "COM1-Name": "COM1", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "COM9-DataBits": "8", "COM1-AnswerTimeout": "500.0000", "Camera": "", "TE2000-Version": "V2.50-V2.50-V2.10", "528 Shutter-Volts": "0.0000", "XLIGHT Hub-Name": "XLIGHT Hub", "PIEZO-Description": "Ludl stage driver adapter", "PositionName": "Pos29", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSampleRate": "100000", "PRIME-FanSpeedSetpoint": "High", "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "405 AO-TriggerSampleRate": "100000", "470 Shutter-Block voltage": "false", "470-State": "0", "COM3-Parity": "None", "405 AO-Volts": "4.5000", "PRIME-TransposeMirrorY": "0", "PRIME-ChipName": "GS144BSI", "470 AO-Volts": "0.0000", "PRIME-TransposeMirrorX": "0", "445 Shutter-SupportsTriggering": "0", "470 Shutter-SupportsTriggering": "0", "XLIGHT Hub-Description": "XLIGHT hub", "PRIME-PreampOffLimit": "10000", "470 AO-Sequenceable": "1", "528 Shutter-MaxVolts": "5.0000", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "Core-XYStage": "XYStage", "470 AO-TriggerSampleRate": "100000", "PRIME-Timing-PreTriggerDelayNs": "24672000.0000", "528-DA Device": "528 Shutter", "Focus-Description": "Nikon TE2000 focus adapter", "FilterBlock-State": "5", "520 AO-Demo": "No", "COM3-DelayBetweenCharsMs": "0.0000", "470 Shutter-Volts": "0.0000", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "PRIME-PP 2 THRESHOLD": "125", "PRIME-PP 4 THRESHOLD": "75", "XYStage-Acceleration": "4.0000", "470 Shutter-Demo": "No", "PRIME-Name": "PMPCIECam00", "640-DA Device": "640 Shutter", "405 AO-Block voltage": "false", "ElapsedTime-ms": 1222947, "PRIME-Offset": "100", "445 AO-IOChannel": "Dev1/ao1", "640 AO-MaxVolts": "5.0000", "PositionIndex": 29, "PRIME-PP 1 ENABLED": "Yes", "COM1-BaudRate": "9600", "640-State": "0", "COM3-Verbose": "1", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "LudlController-TransmissionDelay": "0", "PRIME-FullWellCapacity": "62000", "PRIME-SMARTStreamingEnabled": "No", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "640 Shutter-Description": "NI DAC", "528-Description": "DA device that is used as a shutter", "COM4-Description": "Serial port driver (boost:asio)", "405 Shutter-Description": "NI DAC", "TE2000-Name": "TE2000", "528 Shutter-Description": "NI DAC", "COM9-Description": "Serial port driver (boost:asio)", "LudlController-Name": "LudlController", "445 Shutter-TriggerSequenceLength": "1000", "PRIME-SerialNumber": "A18B203008", "445 AO-Volts": "0.0000", "PRIME-X-dimension": "1200", "PRIME-ShutterCloseDelay": "0", "XLIGHT Disk slider-State": "2", "Position": "Default", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 AO-IOChannel": "Dev1/ao5", "520 AO-IOChannel": "Dev1/ao4", "528 AO-TriggerSequenceLength": "1000", "405 Shutter-MaxVolts": "5.0000", "470-DA Device": "470 Shutter", "PRIME-FTCapable": "No", "Core-ChannelGroup": "Channels", "555 AO-SupportsTriggering": "0", "PRIME-Y-dimension": "1200", "Frame": 0, "555 Shutter-Sequenceable": "1", "PRIME-Timing-ExposureTimeNs": "499998000.0000", "Core-ImageProcessor": "", "528-State": "0", "520 AO-Block voltage": "false", "XLIGHT Excitation Wheel-Label": "QUAD", "640 Shutter-Demo": "No", "COM3-StopBits": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "520 Shutter-IOChannel": "Dev1/ao11", "Width": 1200, "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-SupportsTriggering": "0", "640 AO-TriggerSampleRate": "100000", "520-Description": "DA device that is used as a shutter", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "405 Shutter-Sequenceable": "1", "405 Shutter-SupportsTriggering": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-TriggerSampleRate": "100000", "640 Shutter-MaxVolts": "5.0000", "555 AO-MaxVolts": "5.0000", "520 AO-TriggerSequenceLength": "1000", "COM3-BaudRate": "9600", "COM9-BaudRate": "9600", "Vincent-D3-Channel #": "Ch. #1", "PRIME-PMode": "Normal", "OpticalPath-Label": "Left(100)", "BitDepth": 16, "555 AO-TriggerSequenceLength": "1000", "445 Shutter-Description": "NI DAC", "405 AO-Sequenceable": "1", "PRIME-AcquisitionMethod": "Callbacks", "555 AO-Name": "AnalogIO", "PRIME-ShutterMode": "Pre-Exposure", "COM3-Handshaking": "Off", "445 AO-SupportsTriggering": "0", "ROI": "0-0-1200-1200", "COM9-Verbose": "1", "PRIME-Color": "OFF", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "PRIME-ShutterOpenDelay": "0", "COM1-Parity": "None", "Slice": 1, "SlicePosition": -40, "COM9-DelayBetweenCharsMs": "0.0000", "555-Name": "DA Shutter", "PIEZO-LudlSingleAxisName": "B", "XLIGHT Emission Wheel-Label": "QUAD", "640 Shutter-Name": "AnalogIO", "640 AO-Sequenceable": "1", "520 Shutter-Demo": "No", "528 Shutter-Demo": "No", "470-Name": "DA Shutter", "520-DA Device": "520 Shutter", "XYStage-Description": "Ludl XY stage driver adapter", "445 Shutter-MinVolts": "0.0000", "528 AO-TriggerSampleRate": "100000", "405 AO-MinVolts": "0.0000", "405 Shutter-TriggerSequenceLength": "1000", "Height": 1200, "555 AO-Block voltage": "false", "640 Shutter-Volts": "0.0000", "PixelType": "GRAY16", "445 Shutter-Block voltage": "false", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "520 Shutter-TriggerSequenceLength": "1000", "COM9-Name": "COM9", "Core-SLM": "", "555 AO-Volts": "0.0000", "555 Shutter-Block voltage": "false", "XYStage-Speed": "1800.0000", "520 AO-MaxVolts": "5.0000", "528 Shutter-SupportsTriggering": "0", "COM1-Description": "Serial port driver (boost:asio)", "PRIME-MetadataEnabled": "No", "520 Shutter-MinVolts": "0.0000", "470 Shutter-Sequenceable": "1", "PRIME-PreampDelay": "0", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-MinVolts": "0.0000", "ChannelIndex": 5, "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Core-AutoShutter": "1", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "Exposure-ms": 300, "PRIME-PP 2 ENABLED": "Yes", "640 Shutter-TriggerSampleRate": "100000", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "470 Shutter-IOChannel": "Dev1/ao10", "405 AO-Demo": "No", "PixelSizeUm": 1.1, "470 AO-Demo": "No", "470 Shutter-MaxVolts": "5.0000", "Vincent-D3-Name": "Vincent-D3", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FileName": "img_000000000_P-DAPI-high_001.tif", "LudlController-Reset": "Operate", "PRIME-Exposure": "300.00", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "445-Name": "DA Shutter", "555 Shutter-Name": "AnalogIO", "520 Shutter-TriggerSampleRate": "100000", "445 Shutter-IOChannel": "Dev1/ao9", "PRIME-TransposeCorrection": "0", "XYStage-StepSize": "0.1000", "445-Description": "DA device that is used as a shutter", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 AO-IOChannel": "Dev1/ao7", "520 Shutter-Name": "AnalogIO", "COM3-DataBits": "8", "640 AO-Volts": "0.0000", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "555 Shutter-IOChannel": "Dev1/ao13", "WaitInterval": null, "520 Shutter-Sequenceable": "1", "YPositionUm": -180, "445-State": "0", "445-DA Device": "445 Shutter", "PRIME-CircularBufferAutoSize": "ON", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "555 AO-TriggerSampleRate": "100000", "445 Shutter-Volts": "0.0000", "XLIGHT Hub-Port": "COM3", "Core-AutoFocus": "", "COM4-Handshaking": "Off", "528 Shutter-MinVolts": "0.0000", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "445 Shutter-Name": "AnalogIO", "528 Shutter-Sequenceable": "1", "XYStage-TransposeMirrorX": "0", "XYStage-TransposeMirrorY": "0", "PRIME-ReadoutRate": "100MHz 16bit", "XYStage-Name": "Stage", "640 AO-SupportsTriggering": "0", "640-Name": "DA Shutter", "640 Shutter-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "640 AO-Block voltage": "false", "445 Shutter-Sequenceable": "1", "470 AO-MaxVolts": "5.0000", "COM4-BaudRate": "9600", "470-Description": "DA device that is used as a shutter", "520 Shutter-Description": "NI DAC", "PRIME-CCDTemperature": "-14.8500", "Time": "2019-12-18 23:11:23 -0500", "640 AO-TriggerSequenceLength": "1000", "470 AO-Name": "AnalogIO", "XLIGHT Disk slider-Label": "Disk Pos 40um", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "Binning": "1x1", "TE2000-ModelType": "1", "COM3-AnswerTimeout": "500.0000", "555 Shutter-SupportsTriggering": "0", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "TE2000-Port": "COM4", "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Description": "NI DAC", "COM4-AnswerTimeout": "500.0000", "COM1-DelayBetweenCharsMs": "0.0000", "FilterBlock-Label": "Confocal", "445 AO-Name": "AnalogIO", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-Timing-ReadoutTimeNs": "24672000.0000", "528 AO-Demo": "No", "520 AO-Sequenceable": "1", "555-Description": "DA device that is used as a shutter", "405-DA Device": "405 Shutter", "405 AO-TriggerSequenceLength": "1000", "640 AO-MinVolts": "0.0000", "640 Shutter-Block voltage": "false", "FrameIndex": 0, "PRIME-Port": "CMOS", "555 AO-MinVolts": "0.0000", "COM3-Description": "Serial port driver (boost:asio)", "528 AO-Description": "NI DAC", "555 AO-IOChannel": "Dev1/ao6", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "555 AO-TriggerInputLine": "/Dev1/PFI0", "PRIME-ActualInterval-ms": "390.5200", "LudlController-Port": "COM9", "445 Shutter-Demo": "No", "PRIME-Trigger-Expose Out-Mux": "1", "SliceIndex": 1, "PRIME-CCDTemperatureSetPoint": "-15.0000", "405 Shutter-MinVolts": "0.0000", "PRIME-ExposeOutMode": "First Row", "AxisPositions": null, "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Volts": "0.0000", "528-Name": "DA Shutter", "555-State": "0", "XLIGHT Dichroic-Label": "QUAD", "445 AO-Sequenceable": "1", "405-Name": "DA Shutter", "405 AO-IOChannel": "Dev1/ao0", "Core-Camera": "PRIME", "Focus-Name": "Focus", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "405 Shutter-IOChannel": "Dev1/ao8", "640 AO-Demo": "No", "640 Shutter-SupportsTriggering": "0", "445 AO-Demo": "No", "555-DA Device": "555 Shutter", "470 Shutter-Description": "NI DAC", "528 AO-Volts": "0.0000", "COM1-DataBits": "8", "Vincent-D3-Address": "x", "520 AO-SupportsTriggering": "0", "405-Description": "DA device that is used as a shutter", "640 Shutter-MinVolts": "0.0000", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "405 AO-Name": "AnalogIO", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "640 AO-TriggerInputLine": "/Dev1/PFI0", "405 AO-TriggerInputLine": "/Dev1/PFI0", "640-Description": "DA device that is used as a shutter", "445 Shutter-TriggerSampleRate": "100000", "470 Shutter-TriggerSequenceLength": "1000", "COM1-StopBits": "1", "520 Shutter-SupportsTriggering": "0", "405-State": "1", "Core-Initialize": "1", "XLIGHT Emission Wheel-State": "3", "520 AO-Description": "NI DAC", "528 AO-TriggerInputLine": "/Dev1/PFI0", "445 Shutter-MaxVolts": "5.0000", "COM9-Handshaking": "Off", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "XLIGHT Spin motor-Label": "On (spinning)", "PRIME-PP 5 ENABLED": "No", "UUID": "279125b5-2048-4d1e-95c7-777836c812b6", "PRIME-TriggerMode": "Internal Trigger", "COM1-Handshaking": "Off", "PRIME-Timing-PostTriggerDelayNs": "49344000.0000", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "Focus-Position": "17741.5000", "528 Shutter-Block voltage": "false", "Channel": "P-DAPI-high", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "555 Shutter-Demo": "No", "405 Shutter-Demo": "No", "528 AO-Name": "AnalogIO", "520-State": "0", "Core-Galvo": "", "405 Shutter-TriggerSampleRate": "100000", "Focus-FocusStepSize_(nm)": "100", "PRIME-PixelType": "16bit", "COM4-DataBits": "8", "470 AO-IOChannel": "Dev1/ao2", "520 AO-MinVolts": "0.0000", "COM9-Parity": "None", "PRIME-CircularBufferEnabled": "ON", "PRIME-PP 6 Reset": "No", "640 AO-Description": "NI DAC", "Source": "PRIME", "445 AO-MinVolts": "0.0000", "PIEZO-Name": "Stage", "445 AO-Description": "NI DAC", "PRIME-CircularBufferFrameCount": "46", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "520 AO-Volts": "0.0000", "528 Shutter-TriggerSampleRate": "100000", "XPositionUm": 15712.1, "OpticalPath-State": "4", "PRIME-PP 3 THRESHOLD": "80", "PRIME-PVCAM Version": "3.7.1", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Gain": "1-HDR", "640 Shutter-TriggerSequenceLength": "1000", "528 AO-SupportsTriggering": "0", "445 AO-TriggerInputLine": "/Dev1/PFI0", "OpticalPath-Name": "OpticalPath", "PRIME-PP 4 ENABLED": "Yes", "PRIME-PVCAM Adapter Version": "1.3.29", "COM3-Name": "COM3", "405 AO-MaxVolts": "5.0000", "520 Shutter-Block voltage": "false", "PRIME-Timing-ClearingTimeNs": "49344000.0000", "Core-Focus": "PIEZO", "528 AO-Block voltage": "false", "TE2000-Description": "Nikon TE2000 hub", "528 AO-MinVolts": "0.0000", "COM4-Parity": "None", "Summary": { "Slices": 11, "ChContrastMin": [ 0, 0, 0, 0, 0, 0 ], "PixelAspect": 1, "SlicesFirst": true, "Interval_ms": 0, "Time": "2019-12-18 23:10:50 -0500", "Prefix": "seq_5", "InitialPositionList": [ { "Label": "Pos0", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.21], "Focus": [17741.5], "XYStage": [ 44542.200000000004, 31636.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos1", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.22], "Focus": [17741.4], "XYStage": [ 43801.100000000006, 31637.9 ] }, "GridColumnIndex": 0 }, { "Label": "Pos2", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.5], "XYStage": [ 38561.3, 32040.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos3", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.185], "Focus": [17741.3], "XYStage": [ 37698.4, 32041.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos4", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.16], "Focus": [17741.5], "XYStage": [ 32375.4, 31758.9 ] }, "GridColumnIndex": 0 }, { "Label": "Pos5", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.17000000000002], "Focus": [17741.3], "XYStage": [ 31770.7, 31758.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos6", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.4], "XYStage": [ 26536.800000000003, 31068.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos7", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.205], "Focus": [17741.4], "XYStage": [ 25420.2, 31068.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos8", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.85], "Focus": [17741.3], "XYStage": [ 21059, 31508 ] }, "GridColumnIndex": 0 }, { "Label": "Pos9", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.82], "Focus": [17741.3], "XYStage": [ 19731.9, 31506.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos10", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.79], "Focus": [17741.4], "XYStage": [ 14528.5, 32007.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos11", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.83], "Focus": [17741.2], "XYStage": [ 13830.300000000001, 32009.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos12", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.79], "Focus": [17741.3], "XYStage": [ 8775.300000000001, 32017.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos13", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.815], "Focus": [17741.3], "XYStage": [ 8109.200000000001, 32008.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos14", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.805], "Focus": [17741.3], "XYStage": [ 3483.3, 31908.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos15", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.82], "Focus": [17741.2], "XYStage": [ 2686.8, 31903.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos16", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [211.48000000000002], "Focus": [17741.1], "XYStage": [ -1978.9, 31973.600000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos17", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [211.51500000000001], "Focus": [17741.1], "XYStage": [ -2957.8, 31982.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos18", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.195], "Focus": [17741.5], "XYStage": [ 47747.4, -576.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos19", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.23000000000002], "Focus": [17741.6], "XYStage": [ 47053.9, -576.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos20", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.205], "Focus": [17741.4], "XYStage": [ 41871.200000000004, -479.8 ] }, "GridColumnIndex": 0 }, { "Label": "Pos21", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.4], "XYStage": [ 40882.600000000006, -392 ] }, "GridColumnIndex": 0 }, { "Label": "Pos22", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.24], "Focus": [17741.4], "XYStage": [ 35905.6, -135.3 ] }, "GridColumnIndex": 0 }, { "Label": "Pos23", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.195], "Focus": [17741.5], "XYStage": [ 34990.9, -132.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos24", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.46], "Focus": [17741.4], "XYStage": [ 29678.100000000002, -958.1 ] }, "GridColumnIndex": 0 }, { "Label": "Pos25", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.44], "Focus": [17741.5], "XYStage": [ 28983.600000000002, -939.4000000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos26", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.475], "Focus": [17741.5], "XYStage": [ 23019.7, -122 ] }, "GridColumnIndex": 0 }, { "Label": "Pos27", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.44], "Focus": [17741.7], "XYStage": [ 22428, -116.80000000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos28", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.46], "Focus": [17741.7], "XYStage": [ 16364.900000000001, -181.10000000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos29", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.48000000000002], "Focus": [17741.5], "XYStage": [ 15712.1, -180 ] }, "GridColumnIndex": 0 }, { "Label": "Pos30", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.45000000000002], "Focus": [17741.7], "XYStage": [ 10346.1, 77.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos31", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.49], "Focus": [17741.4], "XYStage": [ 9755.5, 85.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos32", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.475], "Focus": [17741.5], "XYStage": [ 4775.5, 293.90000000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos33", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.445], "Focus": [17741.4], "XYStage": [ 3954.9, 293.90000000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos34", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.455], "Focus": [17741.4], "XYStage": [ -1324.9, 545.6 ] }, "GridColumnIndex": 0 }, { "Label": "Pos35", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.445], "Focus": [17741.3], "XYStage": [ -2097, 576.6 ] }, "GridColumnIndex": 0 } ], "z-step_um": 10, "GridRow": 0, "BitDepth": 16, "Depth": 2, "Channels": 6, "Source": "Micro-Manager", "ChColors": [ -16711681, -256, -65281, -1, -1, -16776961 ], "MetadataVersion": 10, "KeepShutterOpenChannels": true, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536, 65536 ], "CustomIntervals_ms": [], "Height": 1200, "MicroManagerVersion": "1.4.23 20180219", "GridColumn": 0, "UUID": "67598d21-5db5-4842-ab60-f506f7b42c7c", "PixelType": "GRAY16", "Width": 1200, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\Yan\\20191218-YC113-S7S8-miseq", "Frames": 1, "Comment": "", "UserName": "Nikon", "PixelSize_um": 1.1, "ROI": [ 0, 0, 1200, 1200 ], "Date": "2019-12-18", "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC", "P-DAPI-high" ], "KeepShutterOpenSlices": true, "IJType": 1, "ComputerName": "ZadorConfocal2", "Positions": 36, "PositionIndex": 29, "CameraTimeout": 20000, "TimeFirst": false }, "640 AO-Name": "AnalogIO", "Vincent-D3-Command": "Close" }, "FrameKey-0-5-2": { "COM4-StopBits": "1", "528 AO-MaxVolts": "5.0000", "XLIGHT Excitation Wheel-State": "1", "470 Shutter-Name": "AnalogIO", "Core-Shutter": "405", "555 AO-Demo": "No", "XLIGHT Dichroic-State": "2", "XYStage-StepSize-Y": "0.1000", "ZPositionUm": 188.48000000000002, "XYStage-StepSize-X": "0.1000", "PRIME-FirmwareVersion": "20.28", "PRIME-ClearCycles": "2", "520-Name": "DA Shutter", "405 Shutter-Name": "AnalogIO", "PIEZO-StepSize": "0.0050", "PRIME-ClearMode": "Pre-Exposure", "470 AO-MinVolts": "0.0000", "405 AO-SupportsTriggering": "1", "CameraChannelIndex": 0, "XYStage-StartSpeed": "500.0000", "COM9-StopBits": "2", "405 AO-Description": "NI DAC", "COM4-DelayBetweenCharsMs": "0.0000", "445 AO-Block voltage": "false", "520 AO-TriggerInputLine": "/Dev1/PFI0", "555 AO-Description": "NI DAC", "520 AO-TriggerSampleRate": "100000", "COM4-Verbose": "1", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM4-Name": "COM4", "405 Shutter-Volts": "5.0000", "Vincent-D3-Port": "COM1", "PRIME-PP 4 MIN ADU AFFECTED": "200", "470 Shutter-MinVolts": "0.0000", "PRIME-PP 3 ENABLED": "Yes", "470 AO-Description": "NI DAC", "PRIME-Description": "PVCAM API device adapter", "520 Shutter-MaxVolts": "5.0000", "520 Shutter-Volts": "0.0000", "528 AO-Sequenceable": "1", "PRIME-Binning": "1x1", "555 Shutter-MaxVolts": "5.0000", "528 Shutter-TriggerSequenceLength": "1000", "405 Shutter-Block voltage": "false", "XLIGHT Spin motor-State": "1", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "Vincent-D3-Time to open (ms)": "35.0000", "Vincent-D3-Time to close (ms)": "35.0000", "445 AO-TriggerSampleRate": "100000", "640 Shutter-IOChannel": "Dev1/ao14", "555 AO-Sequenceable": "1", "520 AO-Name": "AnalogIO", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "470 AO-Block voltage": "false", "COM1-Verbose": "1", "COM9-AnswerTimeout": "10000.0000", "528 Shutter-Name": "AnalogIO", "PRIME-Trigger Timeout (secs)": "10", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "NextFrame": 0, "470 AO-TriggerSequenceLength": "1000", "PRIME-TransposeXY": "0", "445 AO-TriggerSequenceLength": "1000", "COM1-Name": "COM1", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "COM9-DataBits": "8", "COM1-AnswerTimeout": "500.0000", "Camera": "", "TE2000-Version": "V2.50-V2.50-V2.10", "528 Shutter-Volts": "0.0000", "XLIGHT Hub-Name": "XLIGHT Hub", "PIEZO-Description": "Ludl stage driver adapter", "PositionName": "Pos29", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSampleRate": "100000", "PRIME-FanSpeedSetpoint": "High", "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "405 AO-TriggerSampleRate": "100000", "470 Shutter-Block voltage": "false", "470-State": "0", "COM3-Parity": "None", "405 AO-Volts": "4.5000", "PRIME-TransposeMirrorY": "0", "PRIME-ChipName": "GS144BSI", "470 AO-Volts": "0.0000", "PRIME-TransposeMirrorX": "0", "445 Shutter-SupportsTriggering": "0", "470 Shutter-SupportsTriggering": "0", "XLIGHT Hub-Description": "XLIGHT hub", "PRIME-PreampOffLimit": "10000", "470 AO-Sequenceable": "1", "528 Shutter-MaxVolts": "5.0000", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "Core-XYStage": "XYStage", "470 AO-TriggerSampleRate": "100000", "PRIME-Timing-PreTriggerDelayNs": "24672000.0000", "528-DA Device": "528 Shutter", "Focus-Description": "Nikon TE2000 focus adapter", "FilterBlock-State": "5", "520 AO-Demo": "No", "COM3-DelayBetweenCharsMs": "0.0000", "470 Shutter-Volts": "0.0000", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "PRIME-PP 2 THRESHOLD": "125", "PRIME-PP 4 THRESHOLD": "75", "XYStage-Acceleration": "4.0000", "470 Shutter-Demo": "No", "PRIME-Name": "PMPCIECam00", "640-DA Device": "640 Shutter", "405 AO-Block voltage": "false", "ElapsedTime-ms": 1223528, "PRIME-Offset": "100", "445 AO-IOChannel": "Dev1/ao1", "640 AO-MaxVolts": "5.0000", "PositionIndex": 29, "PRIME-PP 1 ENABLED": "Yes", "COM1-BaudRate": "9600", "640-State": "0", "COM3-Verbose": "1", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "LudlController-TransmissionDelay": "0", "PRIME-FullWellCapacity": "62000", "PRIME-SMARTStreamingEnabled": "No", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "640 Shutter-Description": "NI DAC", "528-Description": "DA device that is used as a shutter", "COM4-Description": "Serial port driver (boost:asio)", "405 Shutter-Description": "NI DAC", "TE2000-Name": "TE2000", "528 Shutter-Description": "NI DAC", "COM9-Description": "Serial port driver (boost:asio)", "LudlController-Name": "LudlController", "445 Shutter-TriggerSequenceLength": "1000", "PRIME-SerialNumber": "A18B203008", "445 AO-Volts": "0.0000", "PRIME-X-dimension": "1200", "PRIME-ShutterCloseDelay": "0", "XLIGHT Disk slider-State": "2", "Position": "Default", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 AO-IOChannel": "Dev1/ao5", "520 AO-IOChannel": "Dev1/ao4", "528 AO-TriggerSequenceLength": "1000", "405 Shutter-MaxVolts": "5.0000", "470-DA Device": "470 Shutter", "PRIME-FTCapable": "No", "Core-ChannelGroup": "Channels", "555 AO-SupportsTriggering": "0", "PRIME-Y-dimension": "1200", "Frame": 0, "555 Shutter-Sequenceable": "1", "PRIME-Timing-ExposureTimeNs": "499998000.0000", "Core-ImageProcessor": "", "528-State": "0", "520 AO-Block voltage": "false", "XLIGHT Excitation Wheel-Label": "QUAD", "640 Shutter-Demo": "No", "COM3-StopBits": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "520 Shutter-IOChannel": "Dev1/ao11", "Width": 1200, "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-SupportsTriggering": "0", "640 AO-TriggerSampleRate": "100000", "520-Description": "DA device that is used as a shutter", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "405 Shutter-Sequenceable": "1", "405 Shutter-SupportsTriggering": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-TriggerSampleRate": "100000", "640 Shutter-MaxVolts": "5.0000", "555 AO-MaxVolts": "5.0000", "520 AO-TriggerSequenceLength": "1000", "COM3-BaudRate": "9600", "COM9-BaudRate": "9600", "Vincent-D3-Channel #": "Ch. #1", "PRIME-PMode": "Normal", "OpticalPath-Label": "Left(100)", "BitDepth": 16, "555 AO-TriggerSequenceLength": "1000", "445 Shutter-Description": "NI DAC", "405 AO-Sequenceable": "1", "PRIME-AcquisitionMethod": "Callbacks", "555 AO-Name": "AnalogIO", "PRIME-ShutterMode": "Pre-Exposure", "COM3-Handshaking": "Off", "445 AO-SupportsTriggering": "0", "ROI": "0-0-1200-1200", "COM9-Verbose": "1", "PRIME-Color": "OFF", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "PRIME-ShutterOpenDelay": "0", "COM1-Parity": "None", "Slice": 2, "SlicePosition": -30, "COM9-DelayBetweenCharsMs": "0.0000", "555-Name": "DA Shutter", "PIEZO-LudlSingleAxisName": "B", "XLIGHT Emission Wheel-Label": "QUAD", "640 Shutter-Name": "AnalogIO", "640 AO-Sequenceable": "1", "520 Shutter-Demo": "No", "528 Shutter-Demo": "No", "470-Name": "DA Shutter", "520-DA Device": "520 Shutter", "XYStage-Description": "Ludl XY stage driver adapter", "445 Shutter-MinVolts": "0.0000", "528 AO-TriggerSampleRate": "100000", "405 AO-MinVolts": "0.0000", "405 Shutter-TriggerSequenceLength": "1000", "Height": 1200, "555 AO-Block voltage": "false", "640 Shutter-Volts": "0.0000", "PixelType": "GRAY16", "445 Shutter-Block voltage": "false", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "520 Shutter-TriggerSequenceLength": "1000", "COM9-Name": "COM9", "Core-SLM": "", "555 AO-Volts": "0.0000", "555 Shutter-Block voltage": "false", "XYStage-Speed": "1800.0000", "520 AO-MaxVolts": "5.0000", "528 Shutter-SupportsTriggering": "0", "COM1-Description": "Serial port driver (boost:asio)", "PRIME-MetadataEnabled": "No", "520 Shutter-MinVolts": "0.0000", "470 Shutter-Sequenceable": "1", "PRIME-PreampDelay": "0", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-MinVolts": "0.0000", "ChannelIndex": 5, "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Core-AutoShutter": "1", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "Exposure-ms": 300, "PRIME-PP 2 ENABLED": "Yes", "640 Shutter-TriggerSampleRate": "100000", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "470 Shutter-IOChannel": "Dev1/ao10", "405 AO-Demo": "No", "PixelSizeUm": 1.1, "470 AO-Demo": "No", "470 Shutter-MaxVolts": "5.0000", "Vincent-D3-Name": "Vincent-D3", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FileName": "img_000000000_P-DAPI-high_002.tif", "LudlController-Reset": "Operate", "PRIME-Exposure": "300.00", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "445-Name": "DA Shutter", "555 Shutter-Name": "AnalogIO", "520 Shutter-TriggerSampleRate": "100000", "445 Shutter-IOChannel": "Dev1/ao9", "PRIME-TransposeCorrection": "0", "XYStage-StepSize": "0.1000", "445-Description": "DA device that is used as a shutter", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 AO-IOChannel": "Dev1/ao7", "520 Shutter-Name": "AnalogIO", "COM3-DataBits": "8", "640 AO-Volts": "0.0000", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "555 Shutter-IOChannel": "Dev1/ao13", "WaitInterval": null, "520 Shutter-Sequenceable": "1", "YPositionUm": -180, "445-State": "0", "445-DA Device": "445 Shutter", "PRIME-CircularBufferAutoSize": "ON", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "555 AO-TriggerSampleRate": "100000", "445 Shutter-Volts": "0.0000", "XLIGHT Hub-Port": "COM3", "Core-AutoFocus": "", "COM4-Handshaking": "Off", "528 Shutter-MinVolts": "0.0000", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "445 Shutter-Name": "AnalogIO", "528 Shutter-Sequenceable": "1", "XYStage-TransposeMirrorX": "0", "XYStage-TransposeMirrorY": "0", "PRIME-ReadoutRate": "100MHz 16bit", "XYStage-Name": "Stage", "640 AO-SupportsTriggering": "0", "640-Name": "DA Shutter", "640 Shutter-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "640 AO-Block voltage": "false", "445 Shutter-Sequenceable": "1", "470 AO-MaxVolts": "5.0000", "COM4-BaudRate": "9600", "470-Description": "DA device that is used as a shutter", "520 Shutter-Description": "NI DAC", "PRIME-CCDTemperature": "-14.8500", "Time": "2019-12-18 23:11:23 -0500", "640 AO-TriggerSequenceLength": "1000", "470 AO-Name": "AnalogIO", "XLIGHT Disk slider-Label": "Disk Pos 40um", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "Binning": "1x1", "TE2000-ModelType": "1", "COM3-AnswerTimeout": "500.0000", "555 Shutter-SupportsTriggering": "0", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "TE2000-Port": "COM4", "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Description": "NI DAC", "COM4-AnswerTimeout": "500.0000", "COM1-DelayBetweenCharsMs": "0.0000", "FilterBlock-Label": "Confocal", "445 AO-Name": "AnalogIO", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-Timing-ReadoutTimeNs": "24672000.0000", "528 AO-Demo": "No", "520 AO-Sequenceable": "1", "555-Description": "DA device that is used as a shutter", "405-DA Device": "405 Shutter", "405 AO-TriggerSequenceLength": "1000", "640 AO-MinVolts": "0.0000", "640 Shutter-Block voltage": "false", "FrameIndex": 0, "PRIME-Port": "CMOS", "555 AO-MinVolts": "0.0000", "COM3-Description": "Serial port driver (boost:asio)", "528 AO-Description": "NI DAC", "555 AO-IOChannel": "Dev1/ao6", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "555 AO-TriggerInputLine": "/Dev1/PFI0", "PRIME-ActualInterval-ms": "390.5200", "LudlController-Port": "COM9", "445 Shutter-Demo": "No", "PRIME-Trigger-Expose Out-Mux": "1", "SliceIndex": 2, "PRIME-CCDTemperatureSetPoint": "-15.0000", "405 Shutter-MinVolts": "0.0000", "PRIME-ExposeOutMode": "First Row", "AxisPositions": null, "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Volts": "0.0000", "528-Name": "DA Shutter", "555-State": "0", "XLIGHT Dichroic-Label": "QUAD", "445 AO-Sequenceable": "1", "405-Name": "DA Shutter", "405 AO-IOChannel": "Dev1/ao0", "Core-Camera": "PRIME", "Focus-Name": "Focus", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "405 Shutter-IOChannel": "Dev1/ao8", "640 AO-Demo": "No", "640 Shutter-SupportsTriggering": "0", "445 AO-Demo": "No", "555-DA Device": "555 Shutter", "470 Shutter-Description": "NI DAC", "528 AO-Volts": "0.0000", "COM1-DataBits": "8", "Vincent-D3-Address": "x", "520 AO-SupportsTriggering": "0", "405-Description": "DA device that is used as a shutter", "640 Shutter-MinVolts": "0.0000", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "405 AO-Name": "AnalogIO", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "640 AO-TriggerInputLine": "/Dev1/PFI0", "405 AO-TriggerInputLine": "/Dev1/PFI0", "640-Description": "DA device that is used as a shutter", "445 Shutter-TriggerSampleRate": "100000", "470 Shutter-TriggerSequenceLength": "1000", "COM1-StopBits": "1", "520 Shutter-SupportsTriggering": "0", "405-State": "1", "Core-Initialize": "1", "XLIGHT Emission Wheel-State": "3", "520 AO-Description": "NI DAC", "528 AO-TriggerInputLine": "/Dev1/PFI0", "445 Shutter-MaxVolts": "5.0000", "COM9-Handshaking": "Off", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "XLIGHT Spin motor-Label": "On (spinning)", "PRIME-PP 5 ENABLED": "No", "UUID": "0187d6e4-cb5b-4830-bbc3-79cafaed1f02", "PRIME-TriggerMode": "Internal Trigger", "COM1-Handshaking": "Off", "PRIME-Timing-PostTriggerDelayNs": "49344000.0000", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "Focus-Position": "17741.5000", "528 Shutter-Block voltage": "false", "Channel": "P-DAPI-high", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "555 Shutter-Demo": "No", "405 Shutter-Demo": "No", "528 AO-Name": "AnalogIO", "520-State": "0", "Core-Galvo": "", "405 Shutter-TriggerSampleRate": "100000", "Focus-FocusStepSize_(nm)": "100", "PRIME-PixelType": "16bit", "COM4-DataBits": "8", "470 AO-IOChannel": "Dev1/ao2", "520 AO-MinVolts": "0.0000", "COM9-Parity": "None", "PRIME-CircularBufferEnabled": "ON", "PRIME-PP 6 Reset": "No", "640 AO-Description": "NI DAC", "Source": "PRIME", "445 AO-MinVolts": "0.0000", "PIEZO-Name": "Stage", "445 AO-Description": "NI DAC", "PRIME-CircularBufferFrameCount": "46", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "520 AO-Volts": "0.0000", "528 Shutter-TriggerSampleRate": "100000", "XPositionUm": 15712.1, "OpticalPath-State": "4", "PRIME-PP 3 THRESHOLD": "80", "PRIME-PVCAM Version": "3.7.1", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Gain": "1-HDR", "640 Shutter-TriggerSequenceLength": "1000", "528 AO-SupportsTriggering": "0", "445 AO-TriggerInputLine": "/Dev1/PFI0", "OpticalPath-Name": "OpticalPath", "PRIME-PP 4 ENABLED": "Yes", "PRIME-PVCAM Adapter Version": "1.3.29", "COM3-Name": "COM3", "405 AO-MaxVolts": "5.0000", "520 Shutter-Block voltage": "false", "PRIME-Timing-ClearingTimeNs": "49344000.0000", "Core-Focus": "PIEZO", "528 AO-Block voltage": "false", "TE2000-Description": "Nikon TE2000 hub", "528 AO-MinVolts": "0.0000", "COM4-Parity": "None", "Summary": { "Slices": 11, "ChContrastMin": [ 0, 0, 0, 0, 0, 0 ], "PixelAspect": 1, "SlicesFirst": true, "Interval_ms": 0, "Time": "2019-12-18 23:10:50 -0500", "Prefix": "seq_5", "InitialPositionList": [ { "Label": "Pos0", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.21], "Focus": [17741.5], "XYStage": [ 44542.200000000004, 31636.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos1", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.22], "Focus": [17741.4], "XYStage": [ 43801.100000000006, 31637.9 ] }, "GridColumnIndex": 0 }, { "Label": "Pos2", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.5], "XYStage": [ 38561.3, 32040.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos3", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.185], "Focus": [17741.3], "XYStage": [ 37698.4, 32041.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos4", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.16], "Focus": [17741.5], "XYStage": [ 32375.4, 31758.9 ] }, "GridColumnIndex": 0 }, { "Label": "Pos5", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.17000000000002], "Focus": [17741.3], "XYStage": [ 31770.7, 31758.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos6", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.4], "XYStage": [ 26536.800000000003, 31068.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos7", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.205], "Focus": [17741.4], "XYStage": [ 25420.2, 31068.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos8", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.85], "Focus": [17741.3], "XYStage": [ 21059, 31508 ] }, "GridColumnIndex": 0 }, { "Label": "Pos9", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.82], "Focus": [17741.3], "XYStage": [ 19731.9, 31506.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos10", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.79], "Focus": [17741.4], "XYStage": [ 14528.5, 32007.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos11", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.83], "Focus": [17741.2], "XYStage": [ 13830.300000000001, 32009.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos12", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.79], "Focus": [17741.3], "XYStage": [ 8775.300000000001, 32017.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos13", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.815], "Focus": [17741.3], "XYStage": [ 8109.200000000001, 32008.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos14", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.805], "Focus": [17741.3], "XYStage": [ 3483.3, 31908.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos15", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.82], "Focus": [17741.2], "XYStage": [ 2686.8, 31903.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos16", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [211.48000000000002], "Focus": [17741.1], "XYStage": [ -1978.9, 31973.600000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos17", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [211.51500000000001], "Focus": [17741.1], "XYStage": [ -2957.8, 31982.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos18", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.195], "Focus": [17741.5], "XYStage": [ 47747.4, -576.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos19", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.23000000000002], "Focus": [17741.6], "XYStage": [ 47053.9, -576.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos20", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.205], "Focus": [17741.4], "XYStage": [ 41871.200000000004, -479.8 ] }, "GridColumnIndex": 0 }, { "Label": "Pos21", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.4], "XYStage": [ 40882.600000000006, -392 ] }, "GridColumnIndex": 0 }, { "Label": "Pos22", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.24], "Focus": [17741.4], "XYStage": [ 35905.6, -135.3 ] }, "GridColumnIndex": 0 }, { "Label": "Pos23", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.195], "Focus": [17741.5], "XYStage": [ 34990.9, -132.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos24", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.46], "Focus": [17741.4], "XYStage": [ 29678.100000000002, -958.1 ] }, "GridColumnIndex": 0 }, { "Label": "Pos25", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.44], "Focus": [17741.5], "XYStage": [ 28983.600000000002, -939.4000000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos26", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.475], "Focus": [17741.5], "XYStage": [ 23019.7, -122 ] }, "GridColumnIndex": 0 }, { "Label": "Pos27", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.44], "Focus": [17741.7], "XYStage": [ 22428, -116.80000000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos28", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.46], "Focus": [17741.7], "XYStage": [ 16364.900000000001, -181.10000000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos29", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.48000000000002], "Focus": [17741.5], "XYStage": [ 15712.1, -180 ] }, "GridColumnIndex": 0 }, { "Label": "Pos30", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.45000000000002], "Focus": [17741.7], "XYStage": [ 10346.1, 77.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos31", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.49], "Focus": [17741.4], "XYStage": [ 9755.5, 85.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos32", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.475], "Focus": [17741.5], "XYStage": [ 4775.5, 293.90000000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos33", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.445], "Focus": [17741.4], "XYStage": [ 3954.9, 293.90000000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos34", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.455], "Focus": [17741.4], "XYStage": [ -1324.9, 545.6 ] }, "GridColumnIndex": 0 }, { "Label": "Pos35", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.445], "Focus": [17741.3], "XYStage": [ -2097, 576.6 ] }, "GridColumnIndex": 0 } ], "z-step_um": 10, "GridRow": 0, "BitDepth": 16, "Depth": 2, "Channels": 6, "Source": "Micro-Manager", "ChColors": [ -16711681, -256, -65281, -1, -1, -16776961 ], "MetadataVersion": 10, "KeepShutterOpenChannels": true, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536, 65536 ], "CustomIntervals_ms": [], "Height": 1200, "MicroManagerVersion": "1.4.23 20180219", "GridColumn": 0, "UUID": "67598d21-5db5-4842-ab60-f506f7b42c7c", "PixelType": "GRAY16", "Width": 1200, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\Yan\\20191218-YC113-S7S8-miseq", "Frames": 1, "Comment": "", "UserName": "Nikon", "PixelSize_um": 1.1, "ROI": [ 0, 0, 1200, 1200 ], "Date": "2019-12-18", "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC", "P-DAPI-high" ], "KeepShutterOpenSlices": true, "IJType": 1, "ComputerName": "ZadorConfocal2", "Positions": 36, "PositionIndex": 29, "CameraTimeout": 20000, "TimeFirst": false }, "640 AO-Name": "AnalogIO", "Vincent-D3-Command": "Close" }, "FrameKey-0-5-3": { "COM4-StopBits": "1", "528 AO-MaxVolts": "5.0000", "XLIGHT Excitation Wheel-State": "1", "470 Shutter-Name": "AnalogIO", "Core-Shutter": "405", "555 AO-Demo": "No", "XLIGHT Dichroic-State": "2", "XYStage-StepSize-Y": "0.1000", "ZPositionUm": 198.48000000000002, "XYStage-StepSize-X": "0.1000", "PRIME-FirmwareVersion": "20.28", "PRIME-ClearCycles": "2", "520-Name": "DA Shutter", "405 Shutter-Name": "AnalogIO", "PIEZO-StepSize": "0.0050", "PRIME-ClearMode": "Pre-Exposure", "470 AO-MinVolts": "0.0000", "405 AO-SupportsTriggering": "1", "CameraChannelIndex": 0, "XYStage-StartSpeed": "500.0000", "COM9-StopBits": "2", "405 AO-Description": "NI DAC", "COM4-DelayBetweenCharsMs": "0.0000", "445 AO-Block voltage": "false", "520 AO-TriggerInputLine": "/Dev1/PFI0", "555 AO-Description": "NI DAC", "520 AO-TriggerSampleRate": "100000", "COM4-Verbose": "1", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM4-Name": "COM4", "405 Shutter-Volts": "5.0000", "Vincent-D3-Port": "COM1", "PRIME-PP 4 MIN ADU AFFECTED": "200", "470 Shutter-MinVolts": "0.0000", "PRIME-PP 3 ENABLED": "Yes", "470 AO-Description": "NI DAC", "PRIME-Description": "PVCAM API device adapter", "520 Shutter-MaxVolts": "5.0000", "520 Shutter-Volts": "0.0000", "528 AO-Sequenceable": "1", "PRIME-Binning": "1x1", "555 Shutter-MaxVolts": "5.0000", "528 Shutter-TriggerSequenceLength": "1000", "405 Shutter-Block voltage": "false", "XLIGHT Spin motor-State": "1", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "Vincent-D3-Time to open (ms)": "35.0000", "Vincent-D3-Time to close (ms)": "35.0000", "445 AO-TriggerSampleRate": "100000", "640 Shutter-IOChannel": "Dev1/ao14", "555 AO-Sequenceable": "1", "520 AO-Name": "AnalogIO", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "470 AO-Block voltage": "false", "COM1-Verbose": "1", "COM9-AnswerTimeout": "10000.0000", "528 Shutter-Name": "AnalogIO", "PRIME-Trigger Timeout (secs)": "10", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "NextFrame": 0, "470 AO-TriggerSequenceLength": "1000", "PRIME-TransposeXY": "0", "445 AO-TriggerSequenceLength": "1000", "COM1-Name": "COM1", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "COM9-DataBits": "8", "COM1-AnswerTimeout": "500.0000", "Camera": "", "TE2000-Version": "V2.50-V2.50-V2.10", "528 Shutter-Volts": "0.0000", "XLIGHT Hub-Name": "XLIGHT Hub", "PIEZO-Description": "Ludl stage driver adapter", "PositionName": "Pos29", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSampleRate": "100000", "PRIME-FanSpeedSetpoint": "High", "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "405 AO-TriggerSampleRate": "100000", "470 Shutter-Block voltage": "false", "470-State": "0", "COM3-Parity": "None", "405 AO-Volts": "4.5000", "PRIME-TransposeMirrorY": "0", "PRIME-ChipName": "GS144BSI", "470 AO-Volts": "0.0000", "PRIME-TransposeMirrorX": "0", "445 Shutter-SupportsTriggering": "0", "470 Shutter-SupportsTriggering": "0", "XLIGHT Hub-Description": "XLIGHT hub", "PRIME-PreampOffLimit": "10000", "470 AO-Sequenceable": "1", "528 Shutter-MaxVolts": "5.0000", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "Core-XYStage": "XYStage", "470 AO-TriggerSampleRate": "100000", "PRIME-Timing-PreTriggerDelayNs": "24672000.0000", "528-DA Device": "528 Shutter", "Focus-Description": "Nikon TE2000 focus adapter", "FilterBlock-State": "5", "520 AO-Demo": "No", "COM3-DelayBetweenCharsMs": "0.0000", "470 Shutter-Volts": "0.0000", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "PRIME-PP 2 THRESHOLD": "125", "PRIME-PP 4 THRESHOLD": "75", "XYStage-Acceleration": "4.0000", "470 Shutter-Demo": "No", "PRIME-Name": "PMPCIECam00", "640-DA Device": "640 Shutter", "405 AO-Block voltage": "false", "ElapsedTime-ms": 1224072, "PRIME-Offset": "100", "445 AO-IOChannel": "Dev1/ao1", "640 AO-MaxVolts": "5.0000", "PositionIndex": 29, "PRIME-PP 1 ENABLED": "Yes", "COM1-BaudRate": "9600", "640-State": "0", "COM3-Verbose": "1", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "LudlController-TransmissionDelay": "0", "PRIME-FullWellCapacity": "62000", "PRIME-SMARTStreamingEnabled": "No", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "640 Shutter-Description": "NI DAC", "528-Description": "DA device that is used as a shutter", "COM4-Description": "Serial port driver (boost:asio)", "405 Shutter-Description": "NI DAC", "TE2000-Name": "TE2000", "528 Shutter-Description": "NI DAC", "COM9-Description": "Serial port driver (boost:asio)", "LudlController-Name": "LudlController", "445 Shutter-TriggerSequenceLength": "1000", "PRIME-SerialNumber": "A18B203008", "445 AO-Volts": "0.0000", "PRIME-X-dimension": "1200", "PRIME-ShutterCloseDelay": "0", "XLIGHT Disk slider-State": "2", "Position": "Default", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 AO-IOChannel": "Dev1/ao5", "520 AO-IOChannel": "Dev1/ao4", "528 AO-TriggerSequenceLength": "1000", "405 Shutter-MaxVolts": "5.0000", "470-DA Device": "470 Shutter", "PRIME-FTCapable": "No", "Core-ChannelGroup": "Channels", "555 AO-SupportsTriggering": "0", "PRIME-Y-dimension": "1200", "Frame": 0, "555 Shutter-Sequenceable": "1", "PRIME-Timing-ExposureTimeNs": "499998000.0000", "Core-ImageProcessor": "", "528-State": "0", "520 AO-Block voltage": "false", "XLIGHT Excitation Wheel-Label": "QUAD", "640 Shutter-Demo": "No", "COM3-StopBits": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "520 Shutter-IOChannel": "Dev1/ao11", "Width": 1200, "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-SupportsTriggering": "0", "640 AO-TriggerSampleRate": "100000", "520-Description": "DA device that is used as a shutter", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "405 Shutter-Sequenceable": "1", "405 Shutter-SupportsTriggering": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-TriggerSampleRate": "100000", "640 Shutter-MaxVolts": "5.0000", "555 AO-MaxVolts": "5.0000", "520 AO-TriggerSequenceLength": "1000", "COM3-BaudRate": "9600", "COM9-BaudRate": "9600", "Vincent-D3-Channel #": "Ch. #1", "PRIME-PMode": "Normal", "OpticalPath-Label": "Left(100)", "BitDepth": 16, "555 AO-TriggerSequenceLength": "1000", "445 Shutter-Description": "NI DAC", "405 AO-Sequenceable": "1", "PRIME-AcquisitionMethod": "Callbacks", "555 AO-Name": "AnalogIO", "PRIME-ShutterMode": "Pre-Exposure", "COM3-Handshaking": "Off", "445 AO-SupportsTriggering": "0", "ROI": "0-0-1200-1200", "COM9-Verbose": "1", "PRIME-Color": "OFF", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "PRIME-ShutterOpenDelay": "0", "COM1-Parity": "None", "Slice": 3, "SlicePosition": -20, "COM9-DelayBetweenCharsMs": "0.0000", "555-Name": "DA Shutter", "PIEZO-LudlSingleAxisName": "B", "XLIGHT Emission Wheel-Label": "QUAD", "640 Shutter-Name": "AnalogIO", "640 AO-Sequenceable": "1", "520 Shutter-Demo": "No", "528 Shutter-Demo": "No", "470-Name": "DA Shutter", "520-DA Device": "520 Shutter", "XYStage-Description": "Ludl XY stage driver adapter", "445 Shutter-MinVolts": "0.0000", "528 AO-TriggerSampleRate": "100000", "405 AO-MinVolts": "0.0000", "405 Shutter-TriggerSequenceLength": "1000", "Height": 1200, "555 AO-Block voltage": "false", "640 Shutter-Volts": "0.0000", "PixelType": "GRAY16", "445 Shutter-Block voltage": "false", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "520 Shutter-TriggerSequenceLength": "1000", "COM9-Name": "COM9", "Core-SLM": "", "555 AO-Volts": "0.0000", "555 Shutter-Block voltage": "false", "XYStage-Speed": "1800.0000", "520 AO-MaxVolts": "5.0000", "528 Shutter-SupportsTriggering": "0", "COM1-Description": "Serial port driver (boost:asio)", "PRIME-MetadataEnabled": "No", "520 Shutter-MinVolts": "0.0000", "470 Shutter-Sequenceable": "1", "PRIME-PreampDelay": "0", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-MinVolts": "0.0000", "ChannelIndex": 5, "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Core-AutoShutter": "1", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "Exposure-ms": 300, "PRIME-PP 2 ENABLED": "Yes", "640 Shutter-TriggerSampleRate": "100000", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "470 Shutter-IOChannel": "Dev1/ao10", "405 AO-Demo": "No", "PixelSizeUm": 1.1, "470 AO-Demo": "No", "470 Shutter-MaxVolts": "5.0000", "Vincent-D3-Name": "Vincent-D3", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FileName": "img_000000000_P-DAPI-high_003.tif", "LudlController-Reset": "Operate", "PRIME-Exposure": "300.00", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "445-Name": "DA Shutter", "555 Shutter-Name": "AnalogIO", "520 Shutter-TriggerSampleRate": "100000", "445 Shutter-IOChannel": "Dev1/ao9", "PRIME-TransposeCorrection": "0", "XYStage-StepSize": "0.1000", "445-Description": "DA device that is used as a shutter", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 AO-IOChannel": "Dev1/ao7", "520 Shutter-Name": "AnalogIO", "COM3-DataBits": "8", "640 AO-Volts": "0.0000", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "555 Shutter-IOChannel": "Dev1/ao13", "WaitInterval": null, "520 Shutter-Sequenceable": "1", "YPositionUm": -180, "445-State": "0", "445-DA Device": "445 Shutter", "PRIME-CircularBufferAutoSize": "ON", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "555 AO-TriggerSampleRate": "100000", "445 Shutter-Volts": "0.0000", "XLIGHT Hub-Port": "COM3", "Core-AutoFocus": "", "COM4-Handshaking": "Off", "528 Shutter-MinVolts": "0.0000", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "445 Shutter-Name": "AnalogIO", "528 Shutter-Sequenceable": "1", "XYStage-TransposeMirrorX": "0", "XYStage-TransposeMirrorY": "0", "PRIME-ReadoutRate": "100MHz 16bit", "XYStage-Name": "Stage", "640 AO-SupportsTriggering": "0", "640-Name": "DA Shutter", "640 Shutter-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "640 AO-Block voltage": "false", "445 Shutter-Sequenceable": "1", "470 AO-MaxVolts": "5.0000", "COM4-BaudRate": "9600", "470-Description": "DA device that is used as a shutter", "520 Shutter-Description": "NI DAC", "PRIME-CCDTemperature": "-14.8500", "Time": "2019-12-18 23:11:24 -0500", "640 AO-TriggerSequenceLength": "1000", "470 AO-Name": "AnalogIO", "XLIGHT Disk slider-Label": "Disk Pos 40um", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "Binning": "1x1", "TE2000-ModelType": "1", "COM3-AnswerTimeout": "500.0000", "555 Shutter-SupportsTriggering": "0", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "TE2000-Port": "COM4", "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Description": "NI DAC", "COM4-AnswerTimeout": "500.0000", "COM1-DelayBetweenCharsMs": "0.0000", "FilterBlock-Label": "Confocal", "445 AO-Name": "AnalogIO", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-Timing-ReadoutTimeNs": "24672000.0000", "528 AO-Demo": "No", "520 AO-Sequenceable": "1", "555-Description": "DA device that is used as a shutter", "405-DA Device": "405 Shutter", "405 AO-TriggerSequenceLength": "1000", "640 AO-MinVolts": "0.0000", "640 Shutter-Block voltage": "false", "FrameIndex": 0, "PRIME-Port": "CMOS", "555 AO-MinVolts": "0.0000", "COM3-Description": "Serial port driver (boost:asio)", "528 AO-Description": "NI DAC", "555 AO-IOChannel": "Dev1/ao6", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "555 AO-TriggerInputLine": "/Dev1/PFI0", "PRIME-ActualInterval-ms": "390.5200", "LudlController-Port": "COM9", "445 Shutter-Demo": "No", "PRIME-Trigger-Expose Out-Mux": "1", "SliceIndex": 3, "PRIME-CCDTemperatureSetPoint": "-15.0000", "405 Shutter-MinVolts": "0.0000", "PRIME-ExposeOutMode": "First Row", "AxisPositions": null, "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Volts": "0.0000", "528-Name": "DA Shutter", "555-State": "0", "XLIGHT Dichroic-Label": "QUAD", "445 AO-Sequenceable": "1", "405-Name": "DA Shutter", "405 AO-IOChannel": "Dev1/ao0", "Core-Camera": "PRIME", "Focus-Name": "Focus", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "405 Shutter-IOChannel": "Dev1/ao8", "640 AO-Demo": "No", "640 Shutter-SupportsTriggering": "0", "445 AO-Demo": "No", "555-DA Device": "555 Shutter", "470 Shutter-Description": "NI DAC", "528 AO-Volts": "0.0000", "COM1-DataBits": "8", "Vincent-D3-Address": "x", "520 AO-SupportsTriggering": "0", "405-Description": "DA device that is used as a shutter", "640 Shutter-MinVolts": "0.0000", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "405 AO-Name": "AnalogIO", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "640 AO-TriggerInputLine": "/Dev1/PFI0", "405 AO-TriggerInputLine": "/Dev1/PFI0", "640-Description": "DA device that is used as a shutter", "445 Shutter-TriggerSampleRate": "100000", "470 Shutter-TriggerSequenceLength": "1000", "COM1-StopBits": "1", "520 Shutter-SupportsTriggering": "0", "405-State": "1", "Core-Initialize": "1", "XLIGHT Emission Wheel-State": "3", "520 AO-Description": "NI DAC", "528 AO-TriggerInputLine": "/Dev1/PFI0", "445 Shutter-MaxVolts": "5.0000", "COM9-Handshaking": "Off", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "XLIGHT Spin motor-Label": "On (spinning)", "PRIME-PP 5 ENABLED": "No", "UUID": "de61ef85-b998-4170-a1af-74e95902e432", "PRIME-TriggerMode": "Internal Trigger", "COM1-Handshaking": "Off", "PRIME-Timing-PostTriggerDelayNs": "49344000.0000", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "Focus-Position": "17741.5000", "528 Shutter-Block voltage": "false", "Channel": "P-DAPI-high", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "555 Shutter-Demo": "No", "405 Shutter-Demo": "No", "528 AO-Name": "AnalogIO", "520-State": "0", "Core-Galvo": "", "405 Shutter-TriggerSampleRate": "100000", "Focus-FocusStepSize_(nm)": "100", "PRIME-PixelType": "16bit", "COM4-DataBits": "8", "470 AO-IOChannel": "Dev1/ao2", "520 AO-MinVolts": "0.0000", "COM9-Parity": "None", "PRIME-CircularBufferEnabled": "ON", "PRIME-PP 6 Reset": "No", "640 AO-Description": "NI DAC", "Source": "PRIME", "445 AO-MinVolts": "0.0000", "PIEZO-Name": "Stage", "445 AO-Description": "NI DAC", "PRIME-CircularBufferFrameCount": "46", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "520 AO-Volts": "0.0000", "528 Shutter-TriggerSampleRate": "100000", "XPositionUm": 15712.1, "OpticalPath-State": "4", "PRIME-PP 3 THRESHOLD": "80", "PRIME-PVCAM Version": "3.7.1", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Gain": "1-HDR", "640 Shutter-TriggerSequenceLength": "1000", "528 AO-SupportsTriggering": "0", "445 AO-TriggerInputLine": "/Dev1/PFI0", "OpticalPath-Name": "OpticalPath", "PRIME-PP 4 ENABLED": "Yes", "PRIME-PVCAM Adapter Version": "1.3.29", "COM3-Name": "COM3", "405 AO-MaxVolts": "5.0000", "520 Shutter-Block voltage": "false", "PRIME-Timing-ClearingTimeNs": "49344000.0000", "Core-Focus": "PIEZO", "528 AO-Block voltage": "false", "TE2000-Description": "Nikon TE2000 hub", "528 AO-MinVolts": "0.0000", "COM4-Parity": "None", "Summary": { "Slices": 11, "ChContrastMin": [ 0, 0, 0, 0, 0, 0 ], "PixelAspect": 1, "SlicesFirst": true, "Interval_ms": 0, "Time": "2019-12-18 23:10:50 -0500", "Prefix": "seq_5", "InitialPositionList": [ { "Label": "Pos0", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.21], "Focus": [17741.5], "XYStage": [ 44542.200000000004, 31636.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos1", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.22], "Focus": [17741.4], "XYStage": [ 43801.100000000006, 31637.9 ] }, "GridColumnIndex": 0 }, { "Label": "Pos2", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.5], "XYStage": [ 38561.3, 32040.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos3", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.185], "Focus": [17741.3], "XYStage": [ 37698.4, 32041.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos4", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.16], "Focus": [17741.5], "XYStage": [ 32375.4, 31758.9 ] }, "GridColumnIndex": 0 }, { "Label": "Pos5", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.17000000000002], "Focus": [17741.3], "XYStage": [ 31770.7, 31758.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos6", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.4], "XYStage": [ 26536.800000000003, 31068.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos7", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.205], "Focus": [17741.4], "XYStage": [ 25420.2, 31068.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos8", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.85], "Focus": [17741.3], "XYStage": [ 21059, 31508 ] }, "GridColumnIndex": 0 }, { "Label": "Pos9", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.82], "Focus": [17741.3], "XYStage": [ 19731.9, 31506.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos10", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.79], "Focus": [17741.4], "XYStage": [ 14528.5, 32007.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos11", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.83], "Focus": [17741.2], "XYStage": [ 13830.300000000001, 32009.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos12", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.79], "Focus": [17741.3], "XYStage": [ 8775.300000000001, 32017.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos13", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.815], "Focus": [17741.3], "XYStage": [ 8109.200000000001, 32008.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos14", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.805], "Focus": [17741.3], "XYStage": [ 3483.3, 31908.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos15", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.82], "Focus": [17741.2], "XYStage": [ 2686.8, 31903.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos16", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [211.48000000000002], "Focus": [17741.1], "XYStage": [ -1978.9, 31973.600000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos17", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [211.51500000000001], "Focus": [17741.1], "XYStage": [ -2957.8, 31982.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos18", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.195], "Focus": [17741.5], "XYStage": [ 47747.4, -576.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos19", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.23000000000002], "Focus": [17741.6], "XYStage": [ 47053.9, -576.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos20", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.205], "Focus": [17741.4], "XYStage": [ 41871.200000000004, -479.8 ] }, "GridColumnIndex": 0 }, { "Label": "Pos21", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.4], "XYStage": [ 40882.600000000006, -392 ] }, "GridColumnIndex": 0 }, { "Label": "Pos22", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.24], "Focus": [17741.4], "XYStage": [ 35905.6, -135.3 ] }, "GridColumnIndex": 0 }, { "Label": "Pos23", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.195], "Focus": [17741.5], "XYStage": [ 34990.9, -132.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos24", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.46], "Focus": [17741.4], "XYStage": [ 29678.100000000002, -958.1 ] }, "GridColumnIndex": 0 }, { "Label": "Pos25", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.44], "Focus": [17741.5], "XYStage": [ 28983.600000000002, -939.4000000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos26", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.475], "Focus": [17741.5], "XYStage": [ 23019.7, -122 ] }, "GridColumnIndex": 0 }, { "Label": "Pos27", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.44], "Focus": [17741.7], "XYStage": [ 22428, -116.80000000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos28", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.46], "Focus": [17741.7], "XYStage": [ 16364.900000000001, -181.10000000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos29", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.48000000000002], "Focus": [17741.5], "XYStage": [ 15712.1, -180 ] }, "GridColumnIndex": 0 }, { "Label": "Pos30", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.45000000000002], "Focus": [17741.7], "XYStage": [ 10346.1, 77.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos31", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.49], "Focus": [17741.4], "XYStage": [ 9755.5, 85.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos32", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.475], "Focus": [17741.5], "XYStage": [ 4775.5, 293.90000000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos33", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.445], "Focus": [17741.4], "XYStage": [ 3954.9, 293.90000000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos34", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.455], "Focus": [17741.4], "XYStage": [ -1324.9, 545.6 ] }, "GridColumnIndex": 0 }, { "Label": "Pos35", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.445], "Focus": [17741.3], "XYStage": [ -2097, 576.6 ] }, "GridColumnIndex": 0 } ], "z-step_um": 10, "GridRow": 0, "BitDepth": 16, "Depth": 2, "Channels": 6, "Source": "Micro-Manager", "ChColors": [ -16711681, -256, -65281, -1, -1, -16776961 ], "MetadataVersion": 10, "KeepShutterOpenChannels": true, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536, 65536 ], "CustomIntervals_ms": [], "Height": 1200, "MicroManagerVersion": "1.4.23 20180219", "GridColumn": 0, "UUID": "67598d21-5db5-4842-ab60-f506f7b42c7c", "PixelType": "GRAY16", "Width": 1200, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\Yan\\20191218-YC113-S7S8-miseq", "Frames": 1, "Comment": "", "UserName": "Nikon", "PixelSize_um": 1.1, "ROI": [ 0, 0, 1200, 1200 ], "Date": "2019-12-18", "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC", "P-DAPI-high" ], "KeepShutterOpenSlices": true, "IJType": 1, "ComputerName": "ZadorConfocal2", "Positions": 36, "PositionIndex": 29, "CameraTimeout": 20000, "TimeFirst": false }, "640 AO-Name": "AnalogIO", "Vincent-D3-Command": "Close" }, "FrameKey-0-5-4": { "COM4-StopBits": "1", "528 AO-MaxVolts": "5.0000", "XLIGHT Excitation Wheel-State": "1", "470 Shutter-Name": "AnalogIO", "Core-Shutter": "405", "555 AO-Demo": "No", "XLIGHT Dichroic-State": "2", "XYStage-StepSize-Y": "0.1000", "ZPositionUm": 208.48000000000002, "XYStage-StepSize-X": "0.1000", "PRIME-FirmwareVersion": "20.28", "PRIME-ClearCycles": "2", "520-Name": "DA Shutter", "405 Shutter-Name": "AnalogIO", "PIEZO-StepSize": "0.0050", "PRIME-ClearMode": "Pre-Exposure", "470 AO-MinVolts": "0.0000", "405 AO-SupportsTriggering": "1", "CameraChannelIndex": 0, "XYStage-StartSpeed": "500.0000", "COM9-StopBits": "2", "405 AO-Description": "NI DAC", "COM4-DelayBetweenCharsMs": "0.0000", "445 AO-Block voltage": "false", "520 AO-TriggerInputLine": "/Dev1/PFI0", "555 AO-Description": "NI DAC", "520 AO-TriggerSampleRate": "100000", "COM4-Verbose": "1", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM4-Name": "COM4", "405 Shutter-Volts": "5.0000", "Vincent-D3-Port": "COM1", "PRIME-PP 4 MIN ADU AFFECTED": "200", "470 Shutter-MinVolts": "0.0000", "PRIME-PP 3 ENABLED": "Yes", "470 AO-Description": "NI DAC", "PRIME-Description": "PVCAM API device adapter", "520 Shutter-MaxVolts": "5.0000", "520 Shutter-Volts": "0.0000", "528 AO-Sequenceable": "1", "PRIME-Binning": "1x1", "555 Shutter-MaxVolts": "5.0000", "528 Shutter-TriggerSequenceLength": "1000", "405 Shutter-Block voltage": "false", "XLIGHT Spin motor-State": "1", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "Vincent-D3-Time to open (ms)": "35.0000", "Vincent-D3-Time to close (ms)": "35.0000", "445 AO-TriggerSampleRate": "100000", "640 Shutter-IOChannel": "Dev1/ao14", "555 AO-Sequenceable": "1", "520 AO-Name": "AnalogIO", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "470 AO-Block voltage": "false", "COM1-Verbose": "1", "COM9-AnswerTimeout": "10000.0000", "528 Shutter-Name": "AnalogIO", "PRIME-Trigger Timeout (secs)": "10", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "NextFrame": 0, "470 AO-TriggerSequenceLength": "1000", "PRIME-TransposeXY": "0", "445 AO-TriggerSequenceLength": "1000", "COM1-Name": "COM1", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "COM9-DataBits": "8", "COM1-AnswerTimeout": "500.0000", "Camera": "", "TE2000-Version": "V2.50-V2.50-V2.10", "528 Shutter-Volts": "0.0000", "XLIGHT Hub-Name": "XLIGHT Hub", "PIEZO-Description": "Ludl stage driver adapter", "PositionName": "Pos29", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSampleRate": "100000", "PRIME-FanSpeedSetpoint": "High", "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "405 AO-TriggerSampleRate": "100000", "470 Shutter-Block voltage": "false", "470-State": "0", "COM3-Parity": "None", "405 AO-Volts": "4.5000", "PRIME-TransposeMirrorY": "0", "PRIME-ChipName": "GS144BSI", "470 AO-Volts": "0.0000", "PRIME-TransposeMirrorX": "0", "445 Shutter-SupportsTriggering": "0", "470 Shutter-SupportsTriggering": "0", "XLIGHT Hub-Description": "XLIGHT hub", "PRIME-PreampOffLimit": "10000", "470 AO-Sequenceable": "1", "528 Shutter-MaxVolts": "5.0000", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "Core-XYStage": "XYStage", "470 AO-TriggerSampleRate": "100000", "PRIME-Timing-PreTriggerDelayNs": "24672000.0000", "528-DA Device": "528 Shutter", "Focus-Description": "Nikon TE2000 focus adapter", "FilterBlock-State": "5", "520 AO-Demo": "No", "COM3-DelayBetweenCharsMs": "0.0000", "470 Shutter-Volts": "0.0000", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "PRIME-PP 2 THRESHOLD": "125", "PRIME-PP 4 THRESHOLD": "75", "XYStage-Acceleration": "4.0000", "470 Shutter-Demo": "No", "PRIME-Name": "PMPCIECam00", "640-DA Device": "640 Shutter", "405 AO-Block voltage": "false", "ElapsedTime-ms": 1224615, "PRIME-Offset": "100", "445 AO-IOChannel": "Dev1/ao1", "640 AO-MaxVolts": "5.0000", "PositionIndex": 29, "PRIME-PP 1 ENABLED": "Yes", "COM1-BaudRate": "9600", "640-State": "0", "COM3-Verbose": "1", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "LudlController-TransmissionDelay": "0", "PRIME-FullWellCapacity": "62000", "PRIME-SMARTStreamingEnabled": "No", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "640 Shutter-Description": "NI DAC", "528-Description": "DA device that is used as a shutter", "COM4-Description": "Serial port driver (boost:asio)", "405 Shutter-Description": "NI DAC", "TE2000-Name": "TE2000", "528 Shutter-Description": "NI DAC", "COM9-Description": "Serial port driver (boost:asio)", "LudlController-Name": "LudlController", "445 Shutter-TriggerSequenceLength": "1000", "PRIME-SerialNumber": "A18B203008", "445 AO-Volts": "0.0000", "PRIME-X-dimension": "1200", "PRIME-ShutterCloseDelay": "0", "XLIGHT Disk slider-State": "2", "Position": "Default", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 AO-IOChannel": "Dev1/ao5", "520 AO-IOChannel": "Dev1/ao4", "528 AO-TriggerSequenceLength": "1000", "405 Shutter-MaxVolts": "5.0000", "470-DA Device": "470 Shutter", "PRIME-FTCapable": "No", "Core-ChannelGroup": "Channels", "555 AO-SupportsTriggering": "0", "PRIME-Y-dimension": "1200", "Frame": 0, "555 Shutter-Sequenceable": "1", "PRIME-Timing-ExposureTimeNs": "499998000.0000", "Core-ImageProcessor": "", "528-State": "0", "520 AO-Block voltage": "false", "XLIGHT Excitation Wheel-Label": "QUAD", "640 Shutter-Demo": "No", "COM3-StopBits": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "520 Shutter-IOChannel": "Dev1/ao11", "Width": 1200, "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-SupportsTriggering": "0", "640 AO-TriggerSampleRate": "100000", "520-Description": "DA device that is used as a shutter", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "405 Shutter-Sequenceable": "1", "405 Shutter-SupportsTriggering": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-TriggerSampleRate": "100000", "640 Shutter-MaxVolts": "5.0000", "555 AO-MaxVolts": "5.0000", "520 AO-TriggerSequenceLength": "1000", "COM3-BaudRate": "9600", "COM9-BaudRate": "9600", "Vincent-D3-Channel #": "Ch. #1", "PRIME-PMode": "Normal", "OpticalPath-Label": "Left(100)", "BitDepth": 16, "555 AO-TriggerSequenceLength": "1000", "445 Shutter-Description": "NI DAC", "405 AO-Sequenceable": "1", "PRIME-AcquisitionMethod": "Callbacks", "555 AO-Name": "AnalogIO", "PRIME-ShutterMode": "Pre-Exposure", "COM3-Handshaking": "Off", "445 AO-SupportsTriggering": "0", "ROI": "0-0-1200-1200", "COM9-Verbose": "1", "PRIME-Color": "OFF", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "PRIME-ShutterOpenDelay": "0", "COM1-Parity": "None", "Slice": 4, "SlicePosition": -10, "COM9-DelayBetweenCharsMs": "0.0000", "555-Name": "DA Shutter", "PIEZO-LudlSingleAxisName": "B", "XLIGHT Emission Wheel-Label": "QUAD", "640 Shutter-Name": "AnalogIO", "640 AO-Sequenceable": "1", "520 Shutter-Demo": "No", "528 Shutter-Demo": "No", "470-Name": "DA Shutter", "520-DA Device": "520 Shutter", "XYStage-Description": "Ludl XY stage driver adapter", "445 Shutter-MinVolts": "0.0000", "528 AO-TriggerSampleRate": "100000", "405 AO-MinVolts": "0.0000", "405 Shutter-TriggerSequenceLength": "1000", "Height": 1200, "555 AO-Block voltage": "false", "640 Shutter-Volts": "0.0000", "PixelType": "GRAY16", "445 Shutter-Block voltage": "false", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "520 Shutter-TriggerSequenceLength": "1000", "COM9-Name": "COM9", "Core-SLM": "", "555 AO-Volts": "0.0000", "555 Shutter-Block voltage": "false", "XYStage-Speed": "1800.0000", "520 AO-MaxVolts": "5.0000", "528 Shutter-SupportsTriggering": "0", "COM1-Description": "Serial port driver (boost:asio)", "PRIME-MetadataEnabled": "No", "520 Shutter-MinVolts": "0.0000", "470 Shutter-Sequenceable": "1", "PRIME-PreampDelay": "0", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-MinVolts": "0.0000", "ChannelIndex": 5, "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Core-AutoShutter": "1", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "Exposure-ms": 300, "PRIME-PP 2 ENABLED": "Yes", "640 Shutter-TriggerSampleRate": "100000", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "470 Shutter-IOChannel": "Dev1/ao10", "405 AO-Demo": "No", "PixelSizeUm": 1.1, "470 AO-Demo": "No", "470 Shutter-MaxVolts": "5.0000", "Vincent-D3-Name": "Vincent-D3", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FileName": "img_000000000_P-DAPI-high_004.tif", "LudlController-Reset": "Operate", "PRIME-Exposure": "300.00", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "445-Name": "DA Shutter", "555 Shutter-Name": "AnalogIO", "520 Shutter-TriggerSampleRate": "100000", "445 Shutter-IOChannel": "Dev1/ao9", "PRIME-TransposeCorrection": "0", "XYStage-StepSize": "0.1000", "445-Description": "DA device that is used as a shutter", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 AO-IOChannel": "Dev1/ao7", "520 Shutter-Name": "AnalogIO", "COM3-DataBits": "8", "640 AO-Volts": "0.0000", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "555 Shutter-IOChannel": "Dev1/ao13", "WaitInterval": null, "520 Shutter-Sequenceable": "1", "YPositionUm": -180, "445-State": "0", "445-DA Device": "445 Shutter", "PRIME-CircularBufferAutoSize": "ON", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "555 AO-TriggerSampleRate": "100000", "445 Shutter-Volts": "0.0000", "XLIGHT Hub-Port": "COM3", "Core-AutoFocus": "", "COM4-Handshaking": "Off", "528 Shutter-MinVolts": "0.0000", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "445 Shutter-Name": "AnalogIO", "528 Shutter-Sequenceable": "1", "XYStage-TransposeMirrorX": "0", "XYStage-TransposeMirrorY": "0", "PRIME-ReadoutRate": "100MHz 16bit", "XYStage-Name": "Stage", "640 AO-SupportsTriggering": "0", "640-Name": "DA Shutter", "640 Shutter-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "640 AO-Block voltage": "false", "445 Shutter-Sequenceable": "1", "470 AO-MaxVolts": "5.0000", "COM4-BaudRate": "9600", "470-Description": "DA device that is used as a shutter", "520 Shutter-Description": "NI DAC", "PRIME-CCDTemperature": "-14.8500", "Time": "2019-12-18 23:11:24 -0500", "640 AO-TriggerSequenceLength": "1000", "470 AO-Name": "AnalogIO", "XLIGHT Disk slider-Label": "Disk Pos 40um", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "Binning": "1x1", "TE2000-ModelType": "1", "COM3-AnswerTimeout": "500.0000", "555 Shutter-SupportsTriggering": "0", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "TE2000-Port": "COM4", "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Description": "NI DAC", "COM4-AnswerTimeout": "500.0000", "COM1-DelayBetweenCharsMs": "0.0000", "FilterBlock-Label": "Confocal", "445 AO-Name": "AnalogIO", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-Timing-ReadoutTimeNs": "24672000.0000", "528 AO-Demo": "No", "520 AO-Sequenceable": "1", "555-Description": "DA device that is used as a shutter", "405-DA Device": "405 Shutter", "405 AO-TriggerSequenceLength": "1000", "640 AO-MinVolts": "0.0000", "640 Shutter-Block voltage": "false", "FrameIndex": 0, "PRIME-Port": "CMOS", "555 AO-MinVolts": "0.0000", "COM3-Description": "Serial port driver (boost:asio)", "528 AO-Description": "NI DAC", "555 AO-IOChannel": "Dev1/ao6", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "555 AO-TriggerInputLine": "/Dev1/PFI0", "PRIME-ActualInterval-ms": "390.5200", "LudlController-Port": "COM9", "445 Shutter-Demo": "No", "PRIME-Trigger-Expose Out-Mux": "1", "SliceIndex": 4, "PRIME-CCDTemperatureSetPoint": "-15.0000", "405 Shutter-MinVolts": "0.0000", "PRIME-ExposeOutMode": "First Row", "AxisPositions": null, "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Volts": "0.0000", "528-Name": "DA Shutter", "555-State": "0", "XLIGHT Dichroic-Label": "QUAD", "445 AO-Sequenceable": "1", "405-Name": "DA Shutter", "405 AO-IOChannel": "Dev1/ao0", "Core-Camera": "PRIME", "Focus-Name": "Focus", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "405 Shutter-IOChannel": "Dev1/ao8", "640 AO-Demo": "No", "640 Shutter-SupportsTriggering": "0", "445 AO-Demo": "No", "555-DA Device": "555 Shutter", "470 Shutter-Description": "NI DAC", "528 AO-Volts": "0.0000", "COM1-DataBits": "8", "Vincent-D3-Address": "x", "520 AO-SupportsTriggering": "0", "405-Description": "DA device that is used as a shutter", "640 Shutter-MinVolts": "0.0000", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "405 AO-Name": "AnalogIO", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "640 AO-TriggerInputLine": "/Dev1/PFI0", "405 AO-TriggerInputLine": "/Dev1/PFI0", "640-Description": "DA device that is used as a shutter", "445 Shutter-TriggerSampleRate": "100000", "470 Shutter-TriggerSequenceLength": "1000", "COM1-StopBits": "1", "520 Shutter-SupportsTriggering": "0", "405-State": "1", "Core-Initialize": "1", "XLIGHT Emission Wheel-State": "3", "520 AO-Description": "NI DAC", "528 AO-TriggerInputLine": "/Dev1/PFI0", "445 Shutter-MaxVolts": "5.0000", "COM9-Handshaking": "Off", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "XLIGHT Spin motor-Label": "On (spinning)", "PRIME-PP 5 ENABLED": "No", "UUID": "bccb1869-9e14-4bdc-81de-e0cf481baecc", "PRIME-TriggerMode": "Internal Trigger", "COM1-Handshaking": "Off", "PRIME-Timing-PostTriggerDelayNs": "49344000.0000", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "Focus-Position": "17741.5000", "528 Shutter-Block voltage": "false", "Channel": "P-DAPI-high", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "555 Shutter-Demo": "No", "405 Shutter-Demo": "No", "528 AO-Name": "AnalogIO", "520-State": "0", "Core-Galvo": "", "405 Shutter-TriggerSampleRate": "100000", "Focus-FocusStepSize_(nm)": "100", "PRIME-PixelType": "16bit", "COM4-DataBits": "8", "470 AO-IOChannel": "Dev1/ao2", "520 AO-MinVolts": "0.0000", "COM9-Parity": "None", "PRIME-CircularBufferEnabled": "ON", "PRIME-PP 6 Reset": "No", "640 AO-Description": "NI DAC", "Source": "PRIME", "445 AO-MinVolts": "0.0000", "PIEZO-Name": "Stage", "445 AO-Description": "NI DAC", "PRIME-CircularBufferFrameCount": "46", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "520 AO-Volts": "0.0000", "528 Shutter-TriggerSampleRate": "100000", "XPositionUm": 15712.1, "OpticalPath-State": "4", "PRIME-PP 3 THRESHOLD": "80", "PRIME-PVCAM Version": "3.7.1", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Gain": "1-HDR", "640 Shutter-TriggerSequenceLength": "1000", "528 AO-SupportsTriggering": "0", "445 AO-TriggerInputLine": "/Dev1/PFI0", "OpticalPath-Name": "OpticalPath", "PRIME-PP 4 ENABLED": "Yes", "PRIME-PVCAM Adapter Version": "1.3.29", "COM3-Name": "COM3", "405 AO-MaxVolts": "5.0000", "520 Shutter-Block voltage": "false", "PRIME-Timing-ClearingTimeNs": "49344000.0000", "Core-Focus": "PIEZO", "528 AO-Block voltage": "false", "TE2000-Description": "Nikon TE2000 hub", "528 AO-MinVolts": "0.0000", "COM4-Parity": "None", "Summary": { "Slices": 11, "ChContrastMin": [ 0, 0, 0, 0, 0, 0 ], "PixelAspect": 1, "SlicesFirst": true, "Interval_ms": 0, "Time": "2019-12-18 23:10:50 -0500", "Prefix": "seq_5", "InitialPositionList": [ { "Label": "Pos0", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.21], "Focus": [17741.5], "XYStage": [ 44542.200000000004, 31636.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos1", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.22], "Focus": [17741.4], "XYStage": [ 43801.100000000006, 31637.9 ] }, "GridColumnIndex": 0 }, { "Label": "Pos2", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.5], "XYStage": [ 38561.3, 32040.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos3", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.185], "Focus": [17741.3], "XYStage": [ 37698.4, 32041.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos4", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.16], "Focus": [17741.5], "XYStage": [ 32375.4, 31758.9 ] }, "GridColumnIndex": 0 }, { "Label": "Pos5", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.17000000000002], "Focus": [17741.3], "XYStage": [ 31770.7, 31758.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos6", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.4], "XYStage": [ 26536.800000000003, 31068.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos7", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.205], "Focus": [17741.4], "XYStage": [ 25420.2, 31068.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos8", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.85], "Focus": [17741.3], "XYStage": [ 21059, 31508 ] }, "GridColumnIndex": 0 }, { "Label": "Pos9", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.82], "Focus": [17741.3], "XYStage": [ 19731.9, 31506.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos10", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.79], "Focus": [17741.4], "XYStage": [ 14528.5, 32007.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos11", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.83], "Focus": [17741.2], "XYStage": [ 13830.300000000001, 32009.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos12", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.79], "Focus": [17741.3], "XYStage": [ 8775.300000000001, 32017.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos13", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.815], "Focus": [17741.3], "XYStage": [ 8109.200000000001, 32008.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos14", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.805], "Focus": [17741.3], "XYStage": [ 3483.3, 31908.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos15", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.82], "Focus": [17741.2], "XYStage": [ 2686.8, 31903.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos16", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [211.48000000000002], "Focus": [17741.1], "XYStage": [ -1978.9, 31973.600000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos17", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [211.51500000000001], "Focus": [17741.1], "XYStage": [ -2957.8, 31982.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos18", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.195], "Focus": [17741.5], "XYStage": [ 47747.4, -576.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos19", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.23000000000002], "Focus": [17741.6], "XYStage": [ 47053.9, -576.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos20", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.205], "Focus": [17741.4], "XYStage": [ 41871.200000000004, -479.8 ] }, "GridColumnIndex": 0 }, { "Label": "Pos21", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.4], "XYStage": [ 40882.600000000006, -392 ] }, "GridColumnIndex": 0 }, { "Label": "Pos22", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.24], "Focus": [17741.4], "XYStage": [ 35905.6, -135.3 ] }, "GridColumnIndex": 0 }, { "Label": "Pos23", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.195], "Focus": [17741.5], "XYStage": [ 34990.9, -132.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos24", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.46], "Focus": [17741.4], "XYStage": [ 29678.100000000002, -958.1 ] }, "GridColumnIndex": 0 }, { "Label": "Pos25", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.44], "Focus": [17741.5], "XYStage": [ 28983.600000000002, -939.4000000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos26", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.475], "Focus": [17741.5], "XYStage": [ 23019.7, -122 ] }, "GridColumnIndex": 0 }, { "Label": "Pos27", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.44], "Focus": [17741.7], "XYStage": [ 22428, -116.80000000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos28", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.46], "Focus": [17741.7], "XYStage": [ 16364.900000000001, -181.10000000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos29", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.48000000000002], "Focus": [17741.5], "XYStage": [ 15712.1, -180 ] }, "GridColumnIndex": 0 }, { "Label": "Pos30", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.45000000000002], "Focus": [17741.7], "XYStage": [ 10346.1, 77.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos31", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.49], "Focus": [17741.4], "XYStage": [ 9755.5, 85.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos32", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.475], "Focus": [17741.5], "XYStage": [ 4775.5, 293.90000000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos33", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.445], "Focus": [17741.4], "XYStage": [ 3954.9, 293.90000000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos34", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.455], "Focus": [17741.4], "XYStage": [ -1324.9, 545.6 ] }, "GridColumnIndex": 0 }, { "Label": "Pos35", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.445], "Focus": [17741.3], "XYStage": [ -2097, 576.6 ] }, "GridColumnIndex": 0 } ], "z-step_um": 10, "GridRow": 0, "BitDepth": 16, "Depth": 2, "Channels": 6, "Source": "Micro-Manager", "ChColors": [ -16711681, -256, -65281, -1, -1, -16776961 ], "MetadataVersion": 10, "KeepShutterOpenChannels": true, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536, 65536 ], "CustomIntervals_ms": [], "Height": 1200, "MicroManagerVersion": "1.4.23 20180219", "GridColumn": 0, "UUID": "67598d21-5db5-4842-ab60-f506f7b42c7c", "PixelType": "GRAY16", "Width": 1200, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\Yan\\20191218-YC113-S7S8-miseq", "Frames": 1, "Comment": "", "UserName": "Nikon", "PixelSize_um": 1.1, "ROI": [ 0, 0, 1200, 1200 ], "Date": "2019-12-18", "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC", "P-DAPI-high" ], "KeepShutterOpenSlices": true, "IJType": 1, "ComputerName": "ZadorConfocal2", "Positions": 36, "PositionIndex": 29, "CameraTimeout": 20000, "TimeFirst": false }, "640 AO-Name": "AnalogIO", "Vincent-D3-Command": "Close" }, "FrameKey-0-5-5": { "COM4-StopBits": "1", "528 AO-MaxVolts": "5.0000", "XLIGHT Excitation Wheel-State": "1", "470 Shutter-Name": "AnalogIO", "Core-Shutter": "405", "555 AO-Demo": "No", "XLIGHT Dichroic-State": "2", "XYStage-StepSize-Y": "0.1000", "ZPositionUm": 218.48000000000002, "XYStage-StepSize-X": "0.1000", "PRIME-FirmwareVersion": "20.28", "PRIME-ClearCycles": "2", "520-Name": "DA Shutter", "405 Shutter-Name": "AnalogIO", "PIEZO-StepSize": "0.0050", "PRIME-ClearMode": "Pre-Exposure", "470 AO-MinVolts": "0.0000", "405 AO-SupportsTriggering": "1", "CameraChannelIndex": 0, "XYStage-StartSpeed": "500.0000", "COM9-StopBits": "2", "405 AO-Description": "NI DAC", "COM4-DelayBetweenCharsMs": "0.0000", "445 AO-Block voltage": "false", "520 AO-TriggerInputLine": "/Dev1/PFI0", "555 AO-Description": "NI DAC", "520 AO-TriggerSampleRate": "100000", "COM4-Verbose": "1", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM4-Name": "COM4", "405 Shutter-Volts": "5.0000", "Vincent-D3-Port": "COM1", "PRIME-PP 4 MIN ADU AFFECTED": "200", "470 Shutter-MinVolts": "0.0000", "PRIME-PP 3 ENABLED": "Yes", "470 AO-Description": "NI DAC", "PRIME-Description": "PVCAM API device adapter", "520 Shutter-MaxVolts": "5.0000", "520 Shutter-Volts": "0.0000", "528 AO-Sequenceable": "1", "PRIME-Binning": "1x1", "555 Shutter-MaxVolts": "5.0000", "528 Shutter-TriggerSequenceLength": "1000", "405 Shutter-Block voltage": "false", "XLIGHT Spin motor-State": "1", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "Vincent-D3-Time to open (ms)": "35.0000", "Vincent-D3-Time to close (ms)": "35.0000", "445 AO-TriggerSampleRate": "100000", "640 Shutter-IOChannel": "Dev1/ao14", "555 AO-Sequenceable": "1", "520 AO-Name": "AnalogIO", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "470 AO-Block voltage": "false", "COM1-Verbose": "1", "COM9-AnswerTimeout": "10000.0000", "528 Shutter-Name": "AnalogIO", "PRIME-Trigger Timeout (secs)": "10", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "NextFrame": 0, "470 AO-TriggerSequenceLength": "1000", "PRIME-TransposeXY": "0", "445 AO-TriggerSequenceLength": "1000", "COM1-Name": "COM1", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "COM9-DataBits": "8", "COM1-AnswerTimeout": "500.0000", "Camera": "", "TE2000-Version": "V2.50-V2.50-V2.10", "528 Shutter-Volts": "0.0000", "XLIGHT Hub-Name": "XLIGHT Hub", "PIEZO-Description": "Ludl stage driver adapter", "PositionName": "Pos29", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSampleRate": "100000", "PRIME-FanSpeedSetpoint": "High", "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "405 AO-TriggerSampleRate": "100000", "470 Shutter-Block voltage": "false", "470-State": "0", "COM3-Parity": "None", "405 AO-Volts": "4.5000", "PRIME-TransposeMirrorY": "0", "PRIME-ChipName": "GS144BSI", "470 AO-Volts": "0.0000", "PRIME-TransposeMirrorX": "0", "445 Shutter-SupportsTriggering": "0", "470 Shutter-SupportsTriggering": "0", "XLIGHT Hub-Description": "XLIGHT hub", "PRIME-PreampOffLimit": "10000", "470 AO-Sequenceable": "1", "528 Shutter-MaxVolts": "5.0000", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "Core-XYStage": "XYStage", "470 AO-TriggerSampleRate": "100000", "PRIME-Timing-PreTriggerDelayNs": "24672000.0000", "528-DA Device": "528 Shutter", "Focus-Description": "Nikon TE2000 focus adapter", "FilterBlock-State": "5", "520 AO-Demo": "No", "COM3-DelayBetweenCharsMs": "0.0000", "470 Shutter-Volts": "0.0000", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "PRIME-PP 2 THRESHOLD": "125", "PRIME-PP 4 THRESHOLD": "75", "XYStage-Acceleration": "4.0000", "470 Shutter-Demo": "No", "PRIME-Name": "PMPCIECam00", "640-DA Device": "640 Shutter", "405 AO-Block voltage": "false", "ElapsedTime-ms": 1225166, "PRIME-Offset": "100", "445 AO-IOChannel": "Dev1/ao1", "640 AO-MaxVolts": "5.0000", "PositionIndex": 29, "PRIME-PP 1 ENABLED": "Yes", "COM1-BaudRate": "9600", "640-State": "0", "COM3-Verbose": "1", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "LudlController-TransmissionDelay": "0", "PRIME-FullWellCapacity": "62000", "PRIME-SMARTStreamingEnabled": "No", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "640 Shutter-Description": "NI DAC", "528-Description": "DA device that is used as a shutter", "COM4-Description": "Serial port driver (boost:asio)", "405 Shutter-Description": "NI DAC", "TE2000-Name": "TE2000", "528 Shutter-Description": "NI DAC", "COM9-Description": "Serial port driver (boost:asio)", "LudlController-Name": "LudlController", "445 Shutter-TriggerSequenceLength": "1000", "PRIME-SerialNumber": "A18B203008", "445 AO-Volts": "0.0000", "PRIME-X-dimension": "1200", "PRIME-ShutterCloseDelay": "0", "XLIGHT Disk slider-State": "2", "Position": "Default", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 AO-IOChannel": "Dev1/ao5", "520 AO-IOChannel": "Dev1/ao4", "528 AO-TriggerSequenceLength": "1000", "405 Shutter-MaxVolts": "5.0000", "470-DA Device": "470 Shutter", "PRIME-FTCapable": "No", "Core-ChannelGroup": "Channels", "555 AO-SupportsTriggering": "0", "PRIME-Y-dimension": "1200", "Frame": 0, "555 Shutter-Sequenceable": "1", "PRIME-Timing-ExposureTimeNs": "499998000.0000", "Core-ImageProcessor": "", "528-State": "0", "520 AO-Block voltage": "false", "XLIGHT Excitation Wheel-Label": "QUAD", "640 Shutter-Demo": "No", "COM3-StopBits": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "520 Shutter-IOChannel": "Dev1/ao11", "Width": 1200, "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-SupportsTriggering": "0", "640 AO-TriggerSampleRate": "100000", "520-Description": "DA device that is used as a shutter", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "405 Shutter-Sequenceable": "1", "405 Shutter-SupportsTriggering": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-TriggerSampleRate": "100000", "640 Shutter-MaxVolts": "5.0000", "555 AO-MaxVolts": "5.0000", "520 AO-TriggerSequenceLength": "1000", "COM3-BaudRate": "9600", "COM9-BaudRate": "9600", "Vincent-D3-Channel #": "Ch. #1", "PRIME-PMode": "Normal", "OpticalPath-Label": "Left(100)", "BitDepth": 16, "555 AO-TriggerSequenceLength": "1000", "445 Shutter-Description": "NI DAC", "405 AO-Sequenceable": "1", "PRIME-AcquisitionMethod": "Callbacks", "555 AO-Name": "AnalogIO", "PRIME-ShutterMode": "Pre-Exposure", "COM3-Handshaking": "Off", "445 AO-SupportsTriggering": "0", "ROI": "0-0-1200-1200", "COM9-Verbose": "1", "PRIME-Color": "OFF", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "PRIME-ShutterOpenDelay": "0", "COM1-Parity": "None", "Slice": 5, "SlicePosition": 0, "COM9-DelayBetweenCharsMs": "0.0000", "555-Name": "DA Shutter", "PIEZO-LudlSingleAxisName": "B", "XLIGHT Emission Wheel-Label": "QUAD", "640 Shutter-Name": "AnalogIO", "640 AO-Sequenceable": "1", "520 Shutter-Demo": "No", "528 Shutter-Demo": "No", "470-Name": "DA Shutter", "520-DA Device": "520 Shutter", "XYStage-Description": "Ludl XY stage driver adapter", "445 Shutter-MinVolts": "0.0000", "528 AO-TriggerSampleRate": "100000", "405 AO-MinVolts": "0.0000", "405 Shutter-TriggerSequenceLength": "1000", "Height": 1200, "555 AO-Block voltage": "false", "640 Shutter-Volts": "0.0000", "PixelType": "GRAY16", "445 Shutter-Block voltage": "false", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "520 Shutter-TriggerSequenceLength": "1000", "COM9-Name": "COM9", "Core-SLM": "", "555 AO-Volts": "0.0000", "555 Shutter-Block voltage": "false", "XYStage-Speed": "1800.0000", "520 AO-MaxVolts": "5.0000", "528 Shutter-SupportsTriggering": "0", "COM1-Description": "Serial port driver (boost:asio)", "PRIME-MetadataEnabled": "No", "520 Shutter-MinVolts": "0.0000", "470 Shutter-Sequenceable": "1", "PRIME-PreampDelay": "0", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-MinVolts": "0.0000", "ChannelIndex": 5, "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Core-AutoShutter": "1", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "Exposure-ms": 300, "PRIME-PP 2 ENABLED": "Yes", "640 Shutter-TriggerSampleRate": "100000", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "470 Shutter-IOChannel": "Dev1/ao10", "405 AO-Demo": "No", "PixelSizeUm": 1.1, "470 AO-Demo": "No", "470 Shutter-MaxVolts": "5.0000", "Vincent-D3-Name": "Vincent-D3", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FileName": "img_000000000_P-DAPI-high_005.tif", "LudlController-Reset": "Operate", "PRIME-Exposure": "300.00", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "445-Name": "DA Shutter", "555 Shutter-Name": "AnalogIO", "520 Shutter-TriggerSampleRate": "100000", "445 Shutter-IOChannel": "Dev1/ao9", "PRIME-TransposeCorrection": "0", "XYStage-StepSize": "0.1000", "445-Description": "DA device that is used as a shutter", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 AO-IOChannel": "Dev1/ao7", "520 Shutter-Name": "AnalogIO", "COM3-DataBits": "8", "640 AO-Volts": "0.0000", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "555 Shutter-IOChannel": "Dev1/ao13", "WaitInterval": null, "520 Shutter-Sequenceable": "1", "YPositionUm": -180, "445-State": "0", "445-DA Device": "445 Shutter", "PRIME-CircularBufferAutoSize": "ON", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "555 AO-TriggerSampleRate": "100000", "445 Shutter-Volts": "0.0000", "XLIGHT Hub-Port": "COM3", "Core-AutoFocus": "", "COM4-Handshaking": "Off", "528 Shutter-MinVolts": "0.0000", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "445 Shutter-Name": "AnalogIO", "528 Shutter-Sequenceable": "1", "XYStage-TransposeMirrorX": "0", "XYStage-TransposeMirrorY": "0", "PRIME-ReadoutRate": "100MHz 16bit", "XYStage-Name": "Stage", "640 AO-SupportsTriggering": "0", "640-Name": "DA Shutter", "640 Shutter-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "640 AO-Block voltage": "false", "445 Shutter-Sequenceable": "1", "470 AO-MaxVolts": "5.0000", "COM4-BaudRate": "9600", "470-Description": "DA device that is used as a shutter", "520 Shutter-Description": "NI DAC", "PRIME-CCDTemperature": "-14.8500", "Time": "2019-12-18 23:11:25 -0500", "640 AO-TriggerSequenceLength": "1000", "470 AO-Name": "AnalogIO", "XLIGHT Disk slider-Label": "Disk Pos 40um", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "Binning": "1x1", "TE2000-ModelType": "1", "COM3-AnswerTimeout": "500.0000", "555 Shutter-SupportsTriggering": "0", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "TE2000-Port": "COM4", "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Description": "NI DAC", "COM4-AnswerTimeout": "500.0000", "COM1-DelayBetweenCharsMs": "0.0000", "FilterBlock-Label": "Confocal", "445 AO-Name": "AnalogIO", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-Timing-ReadoutTimeNs": "24672000.0000", "528 AO-Demo": "No", "520 AO-Sequenceable": "1", "555-Description": "DA device that is used as a shutter", "405-DA Device": "405 Shutter", "405 AO-TriggerSequenceLength": "1000", "640 AO-MinVolts": "0.0000", "640 Shutter-Block voltage": "false", "FrameIndex": 0, "PRIME-Port": "CMOS", "555 AO-MinVolts": "0.0000", "COM3-Description": "Serial port driver (boost:asio)", "528 AO-Description": "NI DAC", "555 AO-IOChannel": "Dev1/ao6", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "555 AO-TriggerInputLine": "/Dev1/PFI0", "PRIME-ActualInterval-ms": "390.5200", "LudlController-Port": "COM9", "445 Shutter-Demo": "No", "PRIME-Trigger-Expose Out-Mux": "1", "SliceIndex": 5, "PRIME-CCDTemperatureSetPoint": "-15.0000", "405 Shutter-MinVolts": "0.0000", "PRIME-ExposeOutMode": "First Row", "AxisPositions": null, "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Volts": "0.0000", "528-Name": "DA Shutter", "555-State": "0", "XLIGHT Dichroic-Label": "QUAD", "445 AO-Sequenceable": "1", "405-Name": "DA Shutter", "405 AO-IOChannel": "Dev1/ao0", "Core-Camera": "PRIME", "Focus-Name": "Focus", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "405 Shutter-IOChannel": "Dev1/ao8", "640 AO-Demo": "No", "640 Shutter-SupportsTriggering": "0", "445 AO-Demo": "No", "555-DA Device": "555 Shutter", "470 Shutter-Description": "NI DAC", "528 AO-Volts": "0.0000", "COM1-DataBits": "8", "Vincent-D3-Address": "x", "520 AO-SupportsTriggering": "0", "405-Description": "DA device that is used as a shutter", "640 Shutter-MinVolts": "0.0000", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "405 AO-Name": "AnalogIO", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "640 AO-TriggerInputLine": "/Dev1/PFI0", "405 AO-TriggerInputLine": "/Dev1/PFI0", "640-Description": "DA device that is used as a shutter", "445 Shutter-TriggerSampleRate": "100000", "470 Shutter-TriggerSequenceLength": "1000", "COM1-StopBits": "1", "520 Shutter-SupportsTriggering": "0", "405-State": "1", "Core-Initialize": "1", "XLIGHT Emission Wheel-State": "3", "520 AO-Description": "NI DAC", "528 AO-TriggerInputLine": "/Dev1/PFI0", "445 Shutter-MaxVolts": "5.0000", "COM9-Handshaking": "Off", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "XLIGHT Spin motor-Label": "On (spinning)", "PRIME-PP 5 ENABLED": "No", "UUID": "241a4332-36cc-47b1-b55f-40dd0216452e", "PRIME-TriggerMode": "Internal Trigger", "COM1-Handshaking": "Off", "PRIME-Timing-PostTriggerDelayNs": "49344000.0000", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "Focus-Position": "17741.5000", "528 Shutter-Block voltage": "false", "Channel": "P-DAPI-high", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "555 Shutter-Demo": "No", "405 Shutter-Demo": "No", "528 AO-Name": "AnalogIO", "520-State": "0", "Core-Galvo": "", "405 Shutter-TriggerSampleRate": "100000", "Focus-FocusStepSize_(nm)": "100", "PRIME-PixelType": "16bit", "COM4-DataBits": "8", "470 AO-IOChannel": "Dev1/ao2", "520 AO-MinVolts": "0.0000", "COM9-Parity": "None", "PRIME-CircularBufferEnabled": "ON", "PRIME-PP 6 Reset": "No", "640 AO-Description": "NI DAC", "Source": "PRIME", "445 AO-MinVolts": "0.0000", "PIEZO-Name": "Stage", "445 AO-Description": "NI DAC", "PRIME-CircularBufferFrameCount": "46", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "520 AO-Volts": "0.0000", "528 Shutter-TriggerSampleRate": "100000", "XPositionUm": 15712.1, "OpticalPath-State": "4", "PRIME-PP 3 THRESHOLD": "80", "PRIME-PVCAM Version": "3.7.1", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Gain": "1-HDR", "640 Shutter-TriggerSequenceLength": "1000", "528 AO-SupportsTriggering": "0", "445 AO-TriggerInputLine": "/Dev1/PFI0", "OpticalPath-Name": "OpticalPath", "PRIME-PP 4 ENABLED": "Yes", "PRIME-PVCAM Adapter Version": "1.3.29", "COM3-Name": "COM3", "405 AO-MaxVolts": "5.0000", "520 Shutter-Block voltage": "false", "PRIME-Timing-ClearingTimeNs": "49344000.0000", "Core-Focus": "PIEZO", "528 AO-Block voltage": "false", "TE2000-Description": "Nikon TE2000 hub", "528 AO-MinVolts": "0.0000", "COM4-Parity": "None", "Summary": { "Slices": 11, "ChContrastMin": [ 0, 0, 0, 0, 0, 0 ], "PixelAspect": 1, "SlicesFirst": true, "Interval_ms": 0, "Time": "2019-12-18 23:10:50 -0500", "Prefix": "seq_5", "InitialPositionList": [ { "Label": "Pos0", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.21], "Focus": [17741.5], "XYStage": [ 44542.200000000004, 31636.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos1", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.22], "Focus": [17741.4], "XYStage": [ 43801.100000000006, 31637.9 ] }, "GridColumnIndex": 0 }, { "Label": "Pos2", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.5], "XYStage": [ 38561.3, 32040.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos3", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.185], "Focus": [17741.3], "XYStage": [ 37698.4, 32041.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos4", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.16], "Focus": [17741.5], "XYStage": [ 32375.4, 31758.9 ] }, "GridColumnIndex": 0 }, { "Label": "Pos5", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.17000000000002], "Focus": [17741.3], "XYStage": [ 31770.7, 31758.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos6", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.4], "XYStage": [ 26536.800000000003, 31068.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos7", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.205], "Focus": [17741.4], "XYStage": [ 25420.2, 31068.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos8", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.85], "Focus": [17741.3], "XYStage": [ 21059, 31508 ] }, "GridColumnIndex": 0 }, { "Label": "Pos9", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.82], "Focus": [17741.3], "XYStage": [ 19731.9, 31506.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos10", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.79], "Focus": [17741.4], "XYStage": [ 14528.5, 32007.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos11", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.83], "Focus": [17741.2], "XYStage": [ 13830.300000000001, 32009.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos12", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.79], "Focus": [17741.3], "XYStage": [ 8775.300000000001, 32017.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos13", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.815], "Focus": [17741.3], "XYStage": [ 8109.200000000001, 32008.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos14", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.805], "Focus": [17741.3], "XYStage": [ 3483.3, 31908.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos15", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.82], "Focus": [17741.2], "XYStage": [ 2686.8, 31903.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos16", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [211.48000000000002], "Focus": [17741.1], "XYStage": [ -1978.9, 31973.600000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos17", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [211.51500000000001], "Focus": [17741.1], "XYStage": [ -2957.8, 31982.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos18", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.195], "Focus": [17741.5], "XYStage": [ 47747.4, -576.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos19", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.23000000000002], "Focus": [17741.6], "XYStage": [ 47053.9, -576.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos20", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.205], "Focus": [17741.4], "XYStage": [ 41871.200000000004, -479.8 ] }, "GridColumnIndex": 0 }, { "Label": "Pos21", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.4], "XYStage": [ 40882.600000000006, -392 ] }, "GridColumnIndex": 0 }, { "Label": "Pos22", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.24], "Focus": [17741.4], "XYStage": [ 35905.6, -135.3 ] }, "GridColumnIndex": 0 }, { "Label": "Pos23", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.195], "Focus": [17741.5], "XYStage": [ 34990.9, -132.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos24", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.46], "Focus": [17741.4], "XYStage": [ 29678.100000000002, -958.1 ] }, "GridColumnIndex": 0 }, { "Label": "Pos25", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.44], "Focus": [17741.5], "XYStage": [ 28983.600000000002, -939.4000000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos26", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.475], "Focus": [17741.5], "XYStage": [ 23019.7, -122 ] }, "GridColumnIndex": 0 }, { "Label": "Pos27", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.44], "Focus": [17741.7], "XYStage": [ 22428, -116.80000000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos28", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.46], "Focus": [17741.7], "XYStage": [ 16364.900000000001, -181.10000000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos29", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.48000000000002], "Focus": [17741.5], "XYStage": [ 15712.1, -180 ] }, "GridColumnIndex": 0 }, { "Label": "Pos30", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.45000000000002], "Focus": [17741.7], "XYStage": [ 10346.1, 77.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos31", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.49], "Focus": [17741.4], "XYStage": [ 9755.5, 85.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos32", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.475], "Focus": [17741.5], "XYStage": [ 4775.5, 293.90000000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos33", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.445], "Focus": [17741.4], "XYStage": [ 3954.9, 293.90000000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos34", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.455], "Focus": [17741.4], "XYStage": [ -1324.9, 545.6 ] }, "GridColumnIndex": 0 }, { "Label": "Pos35", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.445], "Focus": [17741.3], "XYStage": [ -2097, 576.6 ] }, "GridColumnIndex": 0 } ], "z-step_um": 10, "GridRow": 0, "BitDepth": 16, "Depth": 2, "Channels": 6, "Source": "Micro-Manager", "ChColors": [ -16711681, -256, -65281, -1, -1, -16776961 ], "MetadataVersion": 10, "KeepShutterOpenChannels": true, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536, 65536 ], "CustomIntervals_ms": [], "Height": 1200, "MicroManagerVersion": "1.4.23 20180219", "GridColumn": 0, "UUID": "67598d21-5db5-4842-ab60-f506f7b42c7c", "PixelType": "GRAY16", "Width": 1200, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\Yan\\20191218-YC113-S7S8-miseq", "Frames": 1, "Comment": "", "UserName": "Nikon", "PixelSize_um": 1.1, "ROI": [ 0, 0, 1200, 1200 ], "Date": "2019-12-18", "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC", "P-DAPI-high" ], "KeepShutterOpenSlices": true, "IJType": 1, "ComputerName": "ZadorConfocal2", "Positions": 36, "PositionIndex": 29, "CameraTimeout": 20000, "TimeFirst": false }, "640 AO-Name": "AnalogIO", "Vincent-D3-Command": "Close" }, "FrameKey-0-5-6": { "COM4-StopBits": "1", "528 AO-MaxVolts": "5.0000", "XLIGHT Excitation Wheel-State": "1", "470 Shutter-Name": "AnalogIO", "Core-Shutter": "405", "555 AO-Demo": "No", "XLIGHT Dichroic-State": "2", "XYStage-StepSize-Y": "0.1000", "ZPositionUm": 228.48000000000002, "XYStage-StepSize-X": "0.1000", "PRIME-FirmwareVersion": "20.28", "PRIME-ClearCycles": "2", "520-Name": "DA Shutter", "405 Shutter-Name": "AnalogIO", "PIEZO-StepSize": "0.0050", "PRIME-ClearMode": "Pre-Exposure", "470 AO-MinVolts": "0.0000", "405 AO-SupportsTriggering": "1", "CameraChannelIndex": 0, "XYStage-StartSpeed": "500.0000", "COM9-StopBits": "2", "405 AO-Description": "NI DAC", "COM4-DelayBetweenCharsMs": "0.0000", "445 AO-Block voltage": "false", "520 AO-TriggerInputLine": "/Dev1/PFI0", "555 AO-Description": "NI DAC", "520 AO-TriggerSampleRate": "100000", "COM4-Verbose": "1", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM4-Name": "COM4", "405 Shutter-Volts": "5.0000", "Vincent-D3-Port": "COM1", "PRIME-PP 4 MIN ADU AFFECTED": "200", "470 Shutter-MinVolts": "0.0000", "PRIME-PP 3 ENABLED": "Yes", "470 AO-Description": "NI DAC", "PRIME-Description": "PVCAM API device adapter", "520 Shutter-MaxVolts": "5.0000", "520 Shutter-Volts": "0.0000", "528 AO-Sequenceable": "1", "PRIME-Binning": "1x1", "555 Shutter-MaxVolts": "5.0000", "528 Shutter-TriggerSequenceLength": "1000", "405 Shutter-Block voltage": "false", "XLIGHT Spin motor-State": "1", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "Vincent-D3-Time to open (ms)": "35.0000", "Vincent-D3-Time to close (ms)": "35.0000", "445 AO-TriggerSampleRate": "100000", "640 Shutter-IOChannel": "Dev1/ao14", "555 AO-Sequenceable": "1", "520 AO-Name": "AnalogIO", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "470 AO-Block voltage": "false", "COM1-Verbose": "1", "COM9-AnswerTimeout": "10000.0000", "528 Shutter-Name": "AnalogIO", "PRIME-Trigger Timeout (secs)": "10", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "NextFrame": 0, "470 AO-TriggerSequenceLength": "1000", "PRIME-TransposeXY": "0", "445 AO-TriggerSequenceLength": "1000", "COM1-Name": "COM1", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "COM9-DataBits": "8", "COM1-AnswerTimeout": "500.0000", "Camera": "", "TE2000-Version": "V2.50-V2.50-V2.10", "528 Shutter-Volts": "0.0000", "XLIGHT Hub-Name": "XLIGHT Hub", "PIEZO-Description": "Ludl stage driver adapter", "PositionName": "Pos29", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSampleRate": "100000", "PRIME-FanSpeedSetpoint": "High", "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "405 AO-TriggerSampleRate": "100000", "470 Shutter-Block voltage": "false", "470-State": "0", "COM3-Parity": "None", "405 AO-Volts": "4.5000", "PRIME-TransposeMirrorY": "0", "PRIME-ChipName": "GS144BSI", "470 AO-Volts": "0.0000", "PRIME-TransposeMirrorX": "0", "445 Shutter-SupportsTriggering": "0", "470 Shutter-SupportsTriggering": "0", "XLIGHT Hub-Description": "XLIGHT hub", "PRIME-PreampOffLimit": "10000", "470 AO-Sequenceable": "1", "528 Shutter-MaxVolts": "5.0000", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "Core-XYStage": "XYStage", "470 AO-TriggerSampleRate": "100000", "PRIME-Timing-PreTriggerDelayNs": "24672000.0000", "528-DA Device": "528 Shutter", "Focus-Description": "Nikon TE2000 focus adapter", "FilterBlock-State": "5", "520 AO-Demo": "No", "COM3-DelayBetweenCharsMs": "0.0000", "470 Shutter-Volts": "0.0000", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "PRIME-PP 2 THRESHOLD": "125", "PRIME-PP 4 THRESHOLD": "75", "XYStage-Acceleration": "4.0000", "470 Shutter-Demo": "No", "PRIME-Name": "PMPCIECam00", "640-DA Device": "640 Shutter", "405 AO-Block voltage": "false", "ElapsedTime-ms": 1225703, "PRIME-Offset": "100", "445 AO-IOChannel": "Dev1/ao1", "640 AO-MaxVolts": "5.0000", "PositionIndex": 29, "PRIME-PP 1 ENABLED": "Yes", "COM1-BaudRate": "9600", "640-State": "0", "COM3-Verbose": "1", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "LudlController-TransmissionDelay": "0", "PRIME-FullWellCapacity": "62000", "PRIME-SMARTStreamingEnabled": "No", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "640 Shutter-Description": "NI DAC", "528-Description": "DA device that is used as a shutter", "COM4-Description": "Serial port driver (boost:asio)", "405 Shutter-Description": "NI DAC", "TE2000-Name": "TE2000", "528 Shutter-Description": "NI DAC", "COM9-Description": "Serial port driver (boost:asio)", "LudlController-Name": "LudlController", "445 Shutter-TriggerSequenceLength": "1000", "PRIME-SerialNumber": "A18B203008", "445 AO-Volts": "0.0000", "PRIME-X-dimension": "1200", "PRIME-ShutterCloseDelay": "0", "XLIGHT Disk slider-State": "2", "Position": "Default", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 AO-IOChannel": "Dev1/ao5", "520 AO-IOChannel": "Dev1/ao4", "528 AO-TriggerSequenceLength": "1000", "405 Shutter-MaxVolts": "5.0000", "470-DA Device": "470 Shutter", "PRIME-FTCapable": "No", "Core-ChannelGroup": "Channels", "555 AO-SupportsTriggering": "0", "PRIME-Y-dimension": "1200", "Frame": 0, "555 Shutter-Sequenceable": "1", "PRIME-Timing-ExposureTimeNs": "499998000.0000", "Core-ImageProcessor": "", "528-State": "0", "520 AO-Block voltage": "false", "XLIGHT Excitation Wheel-Label": "QUAD", "640 Shutter-Demo": "No", "COM3-StopBits": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "520 Shutter-IOChannel": "Dev1/ao11", "Width": 1200, "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-SupportsTriggering": "0", "640 AO-TriggerSampleRate": "100000", "520-Description": "DA device that is used as a shutter", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "405 Shutter-Sequenceable": "1", "405 Shutter-SupportsTriggering": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-TriggerSampleRate": "100000", "640 Shutter-MaxVolts": "5.0000", "555 AO-MaxVolts": "5.0000", "520 AO-TriggerSequenceLength": "1000", "COM3-BaudRate": "9600", "COM9-BaudRate": "9600", "Vincent-D3-Channel #": "Ch. #1", "PRIME-PMode": "Normal", "OpticalPath-Label": "Left(100)", "BitDepth": 16, "555 AO-TriggerSequenceLength": "1000", "445 Shutter-Description": "NI DAC", "405 AO-Sequenceable": "1", "PRIME-AcquisitionMethod": "Callbacks", "555 AO-Name": "AnalogIO", "PRIME-ShutterMode": "Pre-Exposure", "COM3-Handshaking": "Off", "445 AO-SupportsTriggering": "0", "ROI": "0-0-1200-1200", "COM9-Verbose": "1", "PRIME-Color": "OFF", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "PRIME-ShutterOpenDelay": "0", "COM1-Parity": "None", "Slice": 6, "SlicePosition": 10, "COM9-DelayBetweenCharsMs": "0.0000", "555-Name": "DA Shutter", "PIEZO-LudlSingleAxisName": "B", "XLIGHT Emission Wheel-Label": "QUAD", "640 Shutter-Name": "AnalogIO", "640 AO-Sequenceable": "1", "520 Shutter-Demo": "No", "528 Shutter-Demo": "No", "470-Name": "DA Shutter", "520-DA Device": "520 Shutter", "XYStage-Description": "Ludl XY stage driver adapter", "445 Shutter-MinVolts": "0.0000", "528 AO-TriggerSampleRate": "100000", "405 AO-MinVolts": "0.0000", "405 Shutter-TriggerSequenceLength": "1000", "Height": 1200, "555 AO-Block voltage": "false", "640 Shutter-Volts": "0.0000", "PixelType": "GRAY16", "445 Shutter-Block voltage": "false", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "520 Shutter-TriggerSequenceLength": "1000", "COM9-Name": "COM9", "Core-SLM": "", "555 AO-Volts": "0.0000", "555 Shutter-Block voltage": "false", "XYStage-Speed": "1800.0000", "520 AO-MaxVolts": "5.0000", "528 Shutter-SupportsTriggering": "0", "COM1-Description": "Serial port driver (boost:asio)", "PRIME-MetadataEnabled": "No", "520 Shutter-MinVolts": "0.0000", "470 Shutter-Sequenceable": "1", "PRIME-PreampDelay": "0", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-MinVolts": "0.0000", "ChannelIndex": 5, "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Core-AutoShutter": "1", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "Exposure-ms": 300, "PRIME-PP 2 ENABLED": "Yes", "640 Shutter-TriggerSampleRate": "100000", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "470 Shutter-IOChannel": "Dev1/ao10", "405 AO-Demo": "No", "PixelSizeUm": 1.1, "470 AO-Demo": "No", "470 Shutter-MaxVolts": "5.0000", "Vincent-D3-Name": "Vincent-D3", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FileName": "img_000000000_P-DAPI-high_006.tif", "LudlController-Reset": "Operate", "PRIME-Exposure": "300.00", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "445-Name": "DA Shutter", "555 Shutter-Name": "AnalogIO", "520 Shutter-TriggerSampleRate": "100000", "445 Shutter-IOChannel": "Dev1/ao9", "PRIME-TransposeCorrection": "0", "XYStage-StepSize": "0.1000", "445-Description": "DA device that is used as a shutter", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 AO-IOChannel": "Dev1/ao7", "520 Shutter-Name": "AnalogIO", "COM3-DataBits": "8", "640 AO-Volts": "0.0000", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "555 Shutter-IOChannel": "Dev1/ao13", "WaitInterval": null, "520 Shutter-Sequenceable": "1", "YPositionUm": -180, "445-State": "0", "445-DA Device": "445 Shutter", "PRIME-CircularBufferAutoSize": "ON", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "555 AO-TriggerSampleRate": "100000", "445 Shutter-Volts": "0.0000", "XLIGHT Hub-Port": "COM3", "Core-AutoFocus": "", "COM4-Handshaking": "Off", "528 Shutter-MinVolts": "0.0000", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "445 Shutter-Name": "AnalogIO", "528 Shutter-Sequenceable": "1", "XYStage-TransposeMirrorX": "0", "XYStage-TransposeMirrorY": "0", "PRIME-ReadoutRate": "100MHz 16bit", "XYStage-Name": "Stage", "640 AO-SupportsTriggering": "0", "640-Name": "DA Shutter", "640 Shutter-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "640 AO-Block voltage": "false", "445 Shutter-Sequenceable": "1", "470 AO-MaxVolts": "5.0000", "COM4-BaudRate": "9600", "470-Description": "DA device that is used as a shutter", "520 Shutter-Description": "NI DAC", "PRIME-CCDTemperature": "-14.8500", "Time": "2019-12-18 23:11:25 -0500", "640 AO-TriggerSequenceLength": "1000", "470 AO-Name": "AnalogIO", "XLIGHT Disk slider-Label": "Disk Pos 40um", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "Binning": "1x1", "TE2000-ModelType": "1", "COM3-AnswerTimeout": "500.0000", "555 Shutter-SupportsTriggering": "0", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "TE2000-Port": "COM4", "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Description": "NI DAC", "COM4-AnswerTimeout": "500.0000", "COM1-DelayBetweenCharsMs": "0.0000", "FilterBlock-Label": "Confocal", "445 AO-Name": "AnalogIO", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-Timing-ReadoutTimeNs": "24672000.0000", "528 AO-Demo": "No", "520 AO-Sequenceable": "1", "555-Description": "DA device that is used as a shutter", "405-DA Device": "405 Shutter", "405 AO-TriggerSequenceLength": "1000", "640 AO-MinVolts": "0.0000", "640 Shutter-Block voltage": "false", "FrameIndex": 0, "PRIME-Port": "CMOS", "555 AO-MinVolts": "0.0000", "COM3-Description": "Serial port driver (boost:asio)", "528 AO-Description": "NI DAC", "555 AO-IOChannel": "Dev1/ao6", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "555 AO-TriggerInputLine": "/Dev1/PFI0", "PRIME-ActualInterval-ms": "390.5200", "LudlController-Port": "COM9", "445 Shutter-Demo": "No", "PRIME-Trigger-Expose Out-Mux": "1", "SliceIndex": 6, "PRIME-CCDTemperatureSetPoint": "-15.0000", "405 Shutter-MinVolts": "0.0000", "PRIME-ExposeOutMode": "First Row", "AxisPositions": null, "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Volts": "0.0000", "528-Name": "DA Shutter", "555-State": "0", "XLIGHT Dichroic-Label": "QUAD", "445 AO-Sequenceable": "1", "405-Name": "DA Shutter", "405 AO-IOChannel": "Dev1/ao0", "Core-Camera": "PRIME", "Focus-Name": "Focus", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "405 Shutter-IOChannel": "Dev1/ao8", "640 AO-Demo": "No", "640 Shutter-SupportsTriggering": "0", "445 AO-Demo": "No", "555-DA Device": "555 Shutter", "470 Shutter-Description": "NI DAC", "528 AO-Volts": "0.0000", "COM1-DataBits": "8", "Vincent-D3-Address": "x", "520 AO-SupportsTriggering": "0", "405-Description": "DA device that is used as a shutter", "640 Shutter-MinVolts": "0.0000", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "405 AO-Name": "AnalogIO", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "640 AO-TriggerInputLine": "/Dev1/PFI0", "405 AO-TriggerInputLine": "/Dev1/PFI0", "640-Description": "DA device that is used as a shutter", "445 Shutter-TriggerSampleRate": "100000", "470 Shutter-TriggerSequenceLength": "1000", "COM1-StopBits": "1", "520 Shutter-SupportsTriggering": "0", "405-State": "1", "Core-Initialize": "1", "XLIGHT Emission Wheel-State": "3", "520 AO-Description": "NI DAC", "528 AO-TriggerInputLine": "/Dev1/PFI0", "445 Shutter-MaxVolts": "5.0000", "COM9-Handshaking": "Off", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "XLIGHT Spin motor-Label": "On (spinning)", "PRIME-PP 5 ENABLED": "No", "UUID": "f7803f80-df33-45a5-b780-4299e20e2f7e", "PRIME-TriggerMode": "Internal Trigger", "COM1-Handshaking": "Off", "PRIME-Timing-PostTriggerDelayNs": "49344000.0000", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "Focus-Position": "17741.5000", "528 Shutter-Block voltage": "false", "Channel": "P-DAPI-high", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "555 Shutter-Demo": "No", "405 Shutter-Demo": "No", "528 AO-Name": "AnalogIO", "520-State": "0", "Core-Galvo": "", "405 Shutter-TriggerSampleRate": "100000", "Focus-FocusStepSize_(nm)": "100", "PRIME-PixelType": "16bit", "COM4-DataBits": "8", "470 AO-IOChannel": "Dev1/ao2", "520 AO-MinVolts": "0.0000", "COM9-Parity": "None", "PRIME-CircularBufferEnabled": "ON", "PRIME-PP 6 Reset": "No", "640 AO-Description": "NI DAC", "Source": "PRIME", "445 AO-MinVolts": "0.0000", "PIEZO-Name": "Stage", "445 AO-Description": "NI DAC", "PRIME-CircularBufferFrameCount": "46", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "520 AO-Volts": "0.0000", "528 Shutter-TriggerSampleRate": "100000", "XPositionUm": 15712.1, "OpticalPath-State": "4", "PRIME-PP 3 THRESHOLD": "80", "PRIME-PVCAM Version": "3.7.1", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Gain": "1-HDR", "640 Shutter-TriggerSequenceLength": "1000", "528 AO-SupportsTriggering": "0", "445 AO-TriggerInputLine": "/Dev1/PFI0", "OpticalPath-Name": "OpticalPath", "PRIME-PP 4 ENABLED": "Yes", "PRIME-PVCAM Adapter Version": "1.3.29", "COM3-Name": "COM3", "405 AO-MaxVolts": "5.0000", "520 Shutter-Block voltage": "false", "PRIME-Timing-ClearingTimeNs": "49344000.0000", "Core-Focus": "PIEZO", "528 AO-Block voltage": "false", "TE2000-Description": "Nikon TE2000 hub", "528 AO-MinVolts": "0.0000", "COM4-Parity": "None", "Summary": { "Slices": 11, "ChContrastMin": [ 0, 0, 0, 0, 0, 0 ], "PixelAspect": 1, "SlicesFirst": true, "Interval_ms": 0, "Time": "2019-12-18 23:10:50 -0500", "Prefix": "seq_5", "InitialPositionList": [ { "Label": "Pos0", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.21], "Focus": [17741.5], "XYStage": [ 44542.200000000004, 31636.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos1", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.22], "Focus": [17741.4], "XYStage": [ 43801.100000000006, 31637.9 ] }, "GridColumnIndex": 0 }, { "Label": "Pos2", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.5], "XYStage": [ 38561.3, 32040.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos3", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.185], "Focus": [17741.3], "XYStage": [ 37698.4, 32041.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos4", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.16], "Focus": [17741.5], "XYStage": [ 32375.4, 31758.9 ] }, "GridColumnIndex": 0 }, { "Label": "Pos5", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.17000000000002], "Focus": [17741.3], "XYStage": [ 31770.7, 31758.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos6", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.4], "XYStage": [ 26536.800000000003, 31068.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos7", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.205], "Focus": [17741.4], "XYStage": [ 25420.2, 31068.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos8", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.85], "Focus": [17741.3], "XYStage": [ 21059, 31508 ] }, "GridColumnIndex": 0 }, { "Label": "Pos9", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.82], "Focus": [17741.3], "XYStage": [ 19731.9, 31506.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos10", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.79], "Focus": [17741.4], "XYStage": [ 14528.5, 32007.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos11", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.83], "Focus": [17741.2], "XYStage": [ 13830.300000000001, 32009.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos12", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.79], "Focus": [17741.3], "XYStage": [ 8775.300000000001, 32017.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos13", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.815], "Focus": [17741.3], "XYStage": [ 8109.200000000001, 32008.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos14", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.805], "Focus": [17741.3], "XYStage": [ 3483.3, 31908.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos15", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.82], "Focus": [17741.2], "XYStage": [ 2686.8, 31903.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos16", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [211.48000000000002], "Focus": [17741.1], "XYStage": [ -1978.9, 31973.600000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos17", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [211.51500000000001], "Focus": [17741.1], "XYStage": [ -2957.8, 31982.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos18", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.195], "Focus": [17741.5], "XYStage": [ 47747.4, -576.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos19", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.23000000000002], "Focus": [17741.6], "XYStage": [ 47053.9, -576.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos20", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.205], "Focus": [17741.4], "XYStage": [ 41871.200000000004, -479.8 ] }, "GridColumnIndex": 0 }, { "Label": "Pos21", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.4], "XYStage": [ 40882.600000000006, -392 ] }, "GridColumnIndex": 0 }, { "Label": "Pos22", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.24], "Focus": [17741.4], "XYStage": [ 35905.6, -135.3 ] }, "GridColumnIndex": 0 }, { "Label": "Pos23", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.195], "Focus": [17741.5], "XYStage": [ 34990.9, -132.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos24", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.46], "Focus": [17741.4], "XYStage": [ 29678.100000000002, -958.1 ] }, "GridColumnIndex": 0 }, { "Label": "Pos25", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.44], "Focus": [17741.5], "XYStage": [ 28983.600000000002, -939.4000000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos26", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.475], "Focus": [17741.5], "XYStage": [ 23019.7, -122 ] }, "GridColumnIndex": 0 }, { "Label": "Pos27", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.44], "Focus": [17741.7], "XYStage": [ 22428, -116.80000000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos28", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.46], "Focus": [17741.7], "XYStage": [ 16364.900000000001, -181.10000000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos29", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.48000000000002], "Focus": [17741.5], "XYStage": [ 15712.1, -180 ] }, "GridColumnIndex": 0 }, { "Label": "Pos30", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.45000000000002], "Focus": [17741.7], "XYStage": [ 10346.1, 77.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos31", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.49], "Focus": [17741.4], "XYStage": [ 9755.5, 85.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos32", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.475], "Focus": [17741.5], "XYStage": [ 4775.5, 293.90000000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos33", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.445], "Focus": [17741.4], "XYStage": [ 3954.9, 293.90000000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos34", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.455], "Focus": [17741.4], "XYStage": [ -1324.9, 545.6 ] }, "GridColumnIndex": 0 }, { "Label": "Pos35", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.445], "Focus": [17741.3], "XYStage": [ -2097, 576.6 ] }, "GridColumnIndex": 0 } ], "z-step_um": 10, "GridRow": 0, "BitDepth": 16, "Depth": 2, "Channels": 6, "Source": "Micro-Manager", "ChColors": [ -16711681, -256, -65281, -1, -1, -16776961 ], "MetadataVersion": 10, "KeepShutterOpenChannels": true, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536, 65536 ], "CustomIntervals_ms": [], "Height": 1200, "MicroManagerVersion": "1.4.23 20180219", "GridColumn": 0, "UUID": "67598d21-5db5-4842-ab60-f506f7b42c7c", "PixelType": "GRAY16", "Width": 1200, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\Yan\\20191218-YC113-S7S8-miseq", "Frames": 1, "Comment": "", "UserName": "Nikon", "PixelSize_um": 1.1, "ROI": [ 0, 0, 1200, 1200 ], "Date": "2019-12-18", "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC", "P-DAPI-high" ], "KeepShutterOpenSlices": true, "IJType": 1, "ComputerName": "ZadorConfocal2", "Positions": 36, "PositionIndex": 29, "CameraTimeout": 20000, "TimeFirst": false }, "640 AO-Name": "AnalogIO", "Vincent-D3-Command": "Close" }, "FrameKey-0-5-7": { "COM4-StopBits": "1", "528 AO-MaxVolts": "5.0000", "XLIGHT Excitation Wheel-State": "1", "470 Shutter-Name": "AnalogIO", "Core-Shutter": "405", "555 AO-Demo": "No", "XLIGHT Dichroic-State": "2", "XYStage-StepSize-Y": "0.1000", "ZPositionUm": 238.48000000000002, "XYStage-StepSize-X": "0.1000", "PRIME-FirmwareVersion": "20.28", "PRIME-ClearCycles": "2", "520-Name": "DA Shutter", "405 Shutter-Name": "AnalogIO", "PIEZO-StepSize": "0.0050", "PRIME-ClearMode": "Pre-Exposure", "470 AO-MinVolts": "0.0000", "405 AO-SupportsTriggering": "1", "CameraChannelIndex": 0, "XYStage-StartSpeed": "500.0000", "COM9-StopBits": "2", "405 AO-Description": "NI DAC", "COM4-DelayBetweenCharsMs": "0.0000", "445 AO-Block voltage": "false", "520 AO-TriggerInputLine": "/Dev1/PFI0", "555 AO-Description": "NI DAC", "520 AO-TriggerSampleRate": "100000", "COM4-Verbose": "1", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM4-Name": "COM4", "405 Shutter-Volts": "5.0000", "Vincent-D3-Port": "COM1", "PRIME-PP 4 MIN ADU AFFECTED": "200", "470 Shutter-MinVolts": "0.0000", "PRIME-PP 3 ENABLED": "Yes", "470 AO-Description": "NI DAC", "PRIME-Description": "PVCAM API device adapter", "520 Shutter-MaxVolts": "5.0000", "520 Shutter-Volts": "0.0000", "528 AO-Sequenceable": "1", "PRIME-Binning": "1x1", "555 Shutter-MaxVolts": "5.0000", "528 Shutter-TriggerSequenceLength": "1000", "405 Shutter-Block voltage": "false", "XLIGHT Spin motor-State": "1", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "Vincent-D3-Time to open (ms)": "35.0000", "Vincent-D3-Time to close (ms)": "35.0000", "445 AO-TriggerSampleRate": "100000", "640 Shutter-IOChannel": "Dev1/ao14", "555 AO-Sequenceable": "1", "520 AO-Name": "AnalogIO", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "470 AO-Block voltage": "false", "COM1-Verbose": "1", "COM9-AnswerTimeout": "10000.0000", "528 Shutter-Name": "AnalogIO", "PRIME-Trigger Timeout (secs)": "10", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "NextFrame": 0, "470 AO-TriggerSequenceLength": "1000", "PRIME-TransposeXY": "0", "445 AO-TriggerSequenceLength": "1000", "COM1-Name": "COM1", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "COM9-DataBits": "8", "COM1-AnswerTimeout": "500.0000", "Camera": "", "TE2000-Version": "V2.50-V2.50-V2.10", "528 Shutter-Volts": "0.0000", "XLIGHT Hub-Name": "XLIGHT Hub", "PIEZO-Description": "Ludl stage driver adapter", "PositionName": "Pos29", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSampleRate": "100000", "PRIME-FanSpeedSetpoint": "High", "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "405 AO-TriggerSampleRate": "100000", "470 Shutter-Block voltage": "false", "470-State": "0", "COM3-Parity": "None", "405 AO-Volts": "4.5000", "PRIME-TransposeMirrorY": "0", "PRIME-ChipName": "GS144BSI", "470 AO-Volts": "0.0000", "PRIME-TransposeMirrorX": "0", "445 Shutter-SupportsTriggering": "0", "470 Shutter-SupportsTriggering": "0", "XLIGHT Hub-Description": "XLIGHT hub", "PRIME-PreampOffLimit": "10000", "470 AO-Sequenceable": "1", "528 Shutter-MaxVolts": "5.0000", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "Core-XYStage": "XYStage", "470 AO-TriggerSampleRate": "100000", "PRIME-Timing-PreTriggerDelayNs": "24672000.0000", "528-DA Device": "528 Shutter", "Focus-Description": "Nikon TE2000 focus adapter", "FilterBlock-State": "5", "520 AO-Demo": "No", "COM3-DelayBetweenCharsMs": "0.0000", "470 Shutter-Volts": "0.0000", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "PRIME-PP 2 THRESHOLD": "125", "PRIME-PP 4 THRESHOLD": "75", "XYStage-Acceleration": "4.0000", "470 Shutter-Demo": "No", "PRIME-Name": "PMPCIECam00", "640-DA Device": "640 Shutter", "405 AO-Block voltage": "false", "ElapsedTime-ms": 1226245, "PRIME-Offset": "100", "445 AO-IOChannel": "Dev1/ao1", "640 AO-MaxVolts": "5.0000", "PositionIndex": 29, "PRIME-PP 1 ENABLED": "Yes", "COM1-BaudRate": "9600", "640-State": "0", "COM3-Verbose": "1", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "LudlController-TransmissionDelay": "0", "PRIME-FullWellCapacity": "62000", "PRIME-SMARTStreamingEnabled": "No", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "640 Shutter-Description": "NI DAC", "528-Description": "DA device that is used as a shutter", "COM4-Description": "Serial port driver (boost:asio)", "405 Shutter-Description": "NI DAC", "TE2000-Name": "TE2000", "528 Shutter-Description": "NI DAC", "COM9-Description": "Serial port driver (boost:asio)", "LudlController-Name": "LudlController", "445 Shutter-TriggerSequenceLength": "1000", "PRIME-SerialNumber": "A18B203008", "445 AO-Volts": "0.0000", "PRIME-X-dimension": "1200", "PRIME-ShutterCloseDelay": "0", "XLIGHT Disk slider-State": "2", "Position": "Default", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 AO-IOChannel": "Dev1/ao5", "520 AO-IOChannel": "Dev1/ao4", "528 AO-TriggerSequenceLength": "1000", "405 Shutter-MaxVolts": "5.0000", "470-DA Device": "470 Shutter", "PRIME-FTCapable": "No", "Core-ChannelGroup": "Channels", "555 AO-SupportsTriggering": "0", "PRIME-Y-dimension": "1200", "Frame": 0, "555 Shutter-Sequenceable": "1", "PRIME-Timing-ExposureTimeNs": "499998000.0000", "Core-ImageProcessor": "", "528-State": "0", "520 AO-Block voltage": "false", "XLIGHT Excitation Wheel-Label": "QUAD", "640 Shutter-Demo": "No", "COM3-StopBits": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "520 Shutter-IOChannel": "Dev1/ao11", "Width": 1200, "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-SupportsTriggering": "0", "640 AO-TriggerSampleRate": "100000", "520-Description": "DA device that is used as a shutter", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "405 Shutter-Sequenceable": "1", "405 Shutter-SupportsTriggering": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-TriggerSampleRate": "100000", "640 Shutter-MaxVolts": "5.0000", "555 AO-MaxVolts": "5.0000", "520 AO-TriggerSequenceLength": "1000", "COM3-BaudRate": "9600", "COM9-BaudRate": "9600", "Vincent-D3-Channel #": "Ch. #1", "PRIME-PMode": "Normal", "OpticalPath-Label": "Left(100)", "BitDepth": 16, "555 AO-TriggerSequenceLength": "1000", "445 Shutter-Description": "NI DAC", "405 AO-Sequenceable": "1", "PRIME-AcquisitionMethod": "Callbacks", "555 AO-Name": "AnalogIO", "PRIME-ShutterMode": "Pre-Exposure", "COM3-Handshaking": "Off", "445 AO-SupportsTriggering": "0", "ROI": "0-0-1200-1200", "COM9-Verbose": "1", "PRIME-Color": "OFF", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "PRIME-ShutterOpenDelay": "0", "COM1-Parity": "None", "Slice": 7, "SlicePosition": 20, "COM9-DelayBetweenCharsMs": "0.0000", "555-Name": "DA Shutter", "PIEZO-LudlSingleAxisName": "B", "XLIGHT Emission Wheel-Label": "QUAD", "640 Shutter-Name": "AnalogIO", "640 AO-Sequenceable": "1", "520 Shutter-Demo": "No", "528 Shutter-Demo": "No", "470-Name": "DA Shutter", "520-DA Device": "520 Shutter", "XYStage-Description": "Ludl XY stage driver adapter", "445 Shutter-MinVolts": "0.0000", "528 AO-TriggerSampleRate": "100000", "405 AO-MinVolts": "0.0000", "405 Shutter-TriggerSequenceLength": "1000", "Height": 1200, "555 AO-Block voltage": "false", "640 Shutter-Volts": "0.0000", "PixelType": "GRAY16", "445 Shutter-Block voltage": "false", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "520 Shutter-TriggerSequenceLength": "1000", "COM9-Name": "COM9", "Core-SLM": "", "555 AO-Volts": "0.0000", "555 Shutter-Block voltage": "false", "XYStage-Speed": "1800.0000", "520 AO-MaxVolts": "5.0000", "528 Shutter-SupportsTriggering": "0", "COM1-Description": "Serial port driver (boost:asio)", "PRIME-MetadataEnabled": "No", "520 Shutter-MinVolts": "0.0000", "470 Shutter-Sequenceable": "1", "PRIME-PreampDelay": "0", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-MinVolts": "0.0000", "ChannelIndex": 5, "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Core-AutoShutter": "1", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "Exposure-ms": 300, "PRIME-PP 2 ENABLED": "Yes", "640 Shutter-TriggerSampleRate": "100000", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "470 Shutter-IOChannel": "Dev1/ao10", "405 AO-Demo": "No", "PixelSizeUm": 1.1, "470 AO-Demo": "No", "470 Shutter-MaxVolts": "5.0000", "Vincent-D3-Name": "Vincent-D3", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FileName": "img_000000000_P-DAPI-high_007.tif", "LudlController-Reset": "Operate", "PRIME-Exposure": "300.00", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "445-Name": "DA Shutter", "555 Shutter-Name": "AnalogIO", "520 Shutter-TriggerSampleRate": "100000", "445 Shutter-IOChannel": "Dev1/ao9", "PRIME-TransposeCorrection": "0", "XYStage-StepSize": "0.1000", "445-Description": "DA device that is used as a shutter", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 AO-IOChannel": "Dev1/ao7", "520 Shutter-Name": "AnalogIO", "COM3-DataBits": "8", "640 AO-Volts": "0.0000", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "555 Shutter-IOChannel": "Dev1/ao13", "WaitInterval": null, "520 Shutter-Sequenceable": "1", "YPositionUm": -180, "445-State": "0", "445-DA Device": "445 Shutter", "PRIME-CircularBufferAutoSize": "ON", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "555 AO-TriggerSampleRate": "100000", "445 Shutter-Volts": "0.0000", "XLIGHT Hub-Port": "COM3", "Core-AutoFocus": "", "COM4-Handshaking": "Off", "528 Shutter-MinVolts": "0.0000", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "445 Shutter-Name": "AnalogIO", "528 Shutter-Sequenceable": "1", "XYStage-TransposeMirrorX": "0", "XYStage-TransposeMirrorY": "0", "PRIME-ReadoutRate": "100MHz 16bit", "XYStage-Name": "Stage", "640 AO-SupportsTriggering": "0", "640-Name": "DA Shutter", "640 Shutter-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "640 AO-Block voltage": "false", "445 Shutter-Sequenceable": "1", "470 AO-MaxVolts": "5.0000", "COM4-BaudRate": "9600", "470-Description": "DA device that is used as a shutter", "520 Shutter-Description": "NI DAC", "PRIME-CCDTemperature": "-14.8500", "Time": "2019-12-18 23:11:26 -0500", "640 AO-TriggerSequenceLength": "1000", "470 AO-Name": "AnalogIO", "XLIGHT Disk slider-Label": "Disk Pos 40um", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "Binning": "1x1", "TE2000-ModelType": "1", "COM3-AnswerTimeout": "500.0000", "555 Shutter-SupportsTriggering": "0", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "TE2000-Port": "COM4", "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Description": "NI DAC", "COM4-AnswerTimeout": "500.0000", "COM1-DelayBetweenCharsMs": "0.0000", "FilterBlock-Label": "Confocal", "445 AO-Name": "AnalogIO", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-Timing-ReadoutTimeNs": "24672000.0000", "528 AO-Demo": "No", "520 AO-Sequenceable": "1", "555-Description": "DA device that is used as a shutter", "405-DA Device": "405 Shutter", "405 AO-TriggerSequenceLength": "1000", "640 AO-MinVolts": "0.0000", "640 Shutter-Block voltage": "false", "FrameIndex": 0, "PRIME-Port": "CMOS", "555 AO-MinVolts": "0.0000", "COM3-Description": "Serial port driver (boost:asio)", "528 AO-Description": "NI DAC", "555 AO-IOChannel": "Dev1/ao6", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "555 AO-TriggerInputLine": "/Dev1/PFI0", "PRIME-ActualInterval-ms": "390.5200", "LudlController-Port": "COM9", "445 Shutter-Demo": "No", "PRIME-Trigger-Expose Out-Mux": "1", "SliceIndex": 7, "PRIME-CCDTemperatureSetPoint": "-15.0000", "405 Shutter-MinVolts": "0.0000", "PRIME-ExposeOutMode": "First Row", "AxisPositions": null, "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Volts": "0.0000", "528-Name": "DA Shutter", "555-State": "0", "XLIGHT Dichroic-Label": "QUAD", "445 AO-Sequenceable": "1", "405-Name": "DA Shutter", "405 AO-IOChannel": "Dev1/ao0", "Core-Camera": "PRIME", "Focus-Name": "Focus", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "405 Shutter-IOChannel": "Dev1/ao8", "640 AO-Demo": "No", "640 Shutter-SupportsTriggering": "0", "445 AO-Demo": "No", "555-DA Device": "555 Shutter", "470 Shutter-Description": "NI DAC", "528 AO-Volts": "0.0000", "COM1-DataBits": "8", "Vincent-D3-Address": "x", "520 AO-SupportsTriggering": "0", "405-Description": "DA device that is used as a shutter", "640 Shutter-MinVolts": "0.0000", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "405 AO-Name": "AnalogIO", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "640 AO-TriggerInputLine": "/Dev1/PFI0", "405 AO-TriggerInputLine": "/Dev1/PFI0", "640-Description": "DA device that is used as a shutter", "445 Shutter-TriggerSampleRate": "100000", "470 Shutter-TriggerSequenceLength": "1000", "COM1-StopBits": "1", "520 Shutter-SupportsTriggering": "0", "405-State": "1", "Core-Initialize": "1", "XLIGHT Emission Wheel-State": "3", "520 AO-Description": "NI DAC", "528 AO-TriggerInputLine": "/Dev1/PFI0", "445 Shutter-MaxVolts": "5.0000", "COM9-Handshaking": "Off", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "XLIGHT Spin motor-Label": "On (spinning)", "PRIME-PP 5 ENABLED": "No", "UUID": "b075559e-205d-4432-9aca-a666f8f7cfa9", "PRIME-TriggerMode": "Internal Trigger", "COM1-Handshaking": "Off", "PRIME-Timing-PostTriggerDelayNs": "49344000.0000", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "Focus-Position": "17741.5000", "528 Shutter-Block voltage": "false", "Channel": "P-DAPI-high", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "555 Shutter-Demo": "No", "405 Shutter-Demo": "No", "528 AO-Name": "AnalogIO", "520-State": "0", "Core-Galvo": "", "405 Shutter-TriggerSampleRate": "100000", "Focus-FocusStepSize_(nm)": "100", "PRIME-PixelType": "16bit", "COM4-DataBits": "8", "470 AO-IOChannel": "Dev1/ao2", "520 AO-MinVolts": "0.0000", "COM9-Parity": "None", "PRIME-CircularBufferEnabled": "ON", "PRIME-PP 6 Reset": "No", "640 AO-Description": "NI DAC", "Source": "PRIME", "445 AO-MinVolts": "0.0000", "PIEZO-Name": "Stage", "445 AO-Description": "NI DAC", "PRIME-CircularBufferFrameCount": "46", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "520 AO-Volts": "0.0000", "528 Shutter-TriggerSampleRate": "100000", "XPositionUm": 15712.1, "OpticalPath-State": "4", "PRIME-PP 3 THRESHOLD": "80", "PRIME-PVCAM Version": "3.7.1", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Gain": "1-HDR", "640 Shutter-TriggerSequenceLength": "1000", "528 AO-SupportsTriggering": "0", "445 AO-TriggerInputLine": "/Dev1/PFI0", "OpticalPath-Name": "OpticalPath", "PRIME-PP 4 ENABLED": "Yes", "PRIME-PVCAM Adapter Version": "1.3.29", "COM3-Name": "COM3", "405 AO-MaxVolts": "5.0000", "520 Shutter-Block voltage": "false", "PRIME-Timing-ClearingTimeNs": "49344000.0000", "Core-Focus": "PIEZO", "528 AO-Block voltage": "false", "TE2000-Description": "Nikon TE2000 hub", "528 AO-MinVolts": "0.0000", "COM4-Parity": "None", "Summary": { "Slices": 11, "ChContrastMin": [ 0, 0, 0, 0, 0, 0 ], "PixelAspect": 1, "SlicesFirst": true, "Interval_ms": 0, "Time": "2019-12-18 23:10:50 -0500", "Prefix": "seq_5", "InitialPositionList": [ { "Label": "Pos0", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.21], "Focus": [17741.5], "XYStage": [ 44542.200000000004, 31636.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos1", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.22], "Focus": [17741.4], "XYStage": [ 43801.100000000006, 31637.9 ] }, "GridColumnIndex": 0 }, { "Label": "Pos2", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.5], "XYStage": [ 38561.3, 32040.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos3", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.185], "Focus": [17741.3], "XYStage": [ 37698.4, 32041.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos4", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.16], "Focus": [17741.5], "XYStage": [ 32375.4, 31758.9 ] }, "GridColumnIndex": 0 }, { "Label": "Pos5", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.17000000000002], "Focus": [17741.3], "XYStage": [ 31770.7, 31758.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos6", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.4], "XYStage": [ 26536.800000000003, 31068.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos7", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.205], "Focus": [17741.4], "XYStage": [ 25420.2, 31068.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos8", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.85], "Focus": [17741.3], "XYStage": [ 21059, 31508 ] }, "GridColumnIndex": 0 }, { "Label": "Pos9", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.82], "Focus": [17741.3], "XYStage": [ 19731.9, 31506.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos10", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.79], "Focus": [17741.4], "XYStage": [ 14528.5, 32007.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos11", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.83], "Focus": [17741.2], "XYStage": [ 13830.300000000001, 32009.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos12", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.79], "Focus": [17741.3], "XYStage": [ 8775.300000000001, 32017.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos13", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.815], "Focus": [17741.3], "XYStage": [ 8109.200000000001, 32008.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos14", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.805], "Focus": [17741.3], "XYStage": [ 3483.3, 31908.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos15", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.82], "Focus": [17741.2], "XYStage": [ 2686.8, 31903.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos16", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [211.48000000000002], "Focus": [17741.1], "XYStage": [ -1978.9, 31973.600000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos17", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [211.51500000000001], "Focus": [17741.1], "XYStage": [ -2957.8, 31982.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos18", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.195], "Focus": [17741.5], "XYStage": [ 47747.4, -576.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos19", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.23000000000002], "Focus": [17741.6], "XYStage": [ 47053.9, -576.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos20", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.205], "Focus": [17741.4], "XYStage": [ 41871.200000000004, -479.8 ] }, "GridColumnIndex": 0 }, { "Label": "Pos21", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.4], "XYStage": [ 40882.600000000006, -392 ] }, "GridColumnIndex": 0 }, { "Label": "Pos22", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.24], "Focus": [17741.4], "XYStage": [ 35905.6, -135.3 ] }, "GridColumnIndex": 0 }, { "Label": "Pos23", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.195], "Focus": [17741.5], "XYStage": [ 34990.9, -132.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos24", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.46], "Focus": [17741.4], "XYStage": [ 29678.100000000002, -958.1 ] }, "GridColumnIndex": 0 }, { "Label": "Pos25", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.44], "Focus": [17741.5], "XYStage": [ 28983.600000000002, -939.4000000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos26", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.475], "Focus": [17741.5], "XYStage": [ 23019.7, -122 ] }, "GridColumnIndex": 0 }, { "Label": "Pos27", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.44], "Focus": [17741.7], "XYStage": [ 22428, -116.80000000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos28", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.46], "Focus": [17741.7], "XYStage": [ 16364.900000000001, -181.10000000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos29", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.48000000000002], "Focus": [17741.5], "XYStage": [ 15712.1, -180 ] }, "GridColumnIndex": 0 }, { "Label": "Pos30", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.45000000000002], "Focus": [17741.7], "XYStage": [ 10346.1, 77.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos31", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.49], "Focus": [17741.4], "XYStage": [ 9755.5, 85.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos32", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.475], "Focus": [17741.5], "XYStage": [ 4775.5, 293.90000000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos33", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.445], "Focus": [17741.4], "XYStage": [ 3954.9, 293.90000000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos34", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.455], "Focus": [17741.4], "XYStage": [ -1324.9, 545.6 ] }, "GridColumnIndex": 0 }, { "Label": "Pos35", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.445], "Focus": [17741.3], "XYStage": [ -2097, 576.6 ] }, "GridColumnIndex": 0 } ], "z-step_um": 10, "GridRow": 0, "BitDepth": 16, "Depth": 2, "Channels": 6, "Source": "Micro-Manager", "ChColors": [ -16711681, -256, -65281, -1, -1, -16776961 ], "MetadataVersion": 10, "KeepShutterOpenChannels": true, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536, 65536 ], "CustomIntervals_ms": [], "Height": 1200, "MicroManagerVersion": "1.4.23 20180219", "GridColumn": 0, "UUID": "67598d21-5db5-4842-ab60-f506f7b42c7c", "PixelType": "GRAY16", "Width": 1200, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\Yan\\20191218-YC113-S7S8-miseq", "Frames": 1, "Comment": "", "UserName": "Nikon", "PixelSize_um": 1.1, "ROI": [ 0, 0, 1200, 1200 ], "Date": "2019-12-18", "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC", "P-DAPI-high" ], "KeepShutterOpenSlices": true, "IJType": 1, "ComputerName": "ZadorConfocal2", "Positions": 36, "PositionIndex": 29, "CameraTimeout": 20000, "TimeFirst": false }, "640 AO-Name": "AnalogIO", "Vincent-D3-Command": "Close" }, "FrameKey-0-5-8": { "COM4-StopBits": "1", "528 AO-MaxVolts": "5.0000", "XLIGHT Excitation Wheel-State": "1", "470 Shutter-Name": "AnalogIO", "Core-Shutter": "405", "555 AO-Demo": "No", "XLIGHT Dichroic-State": "2", "XYStage-StepSize-Y": "0.1000", "ZPositionUm": 248.48000000000002, "XYStage-StepSize-X": "0.1000", "PRIME-FirmwareVersion": "20.28", "PRIME-ClearCycles": "2", "520-Name": "DA Shutter", "405 Shutter-Name": "AnalogIO", "PIEZO-StepSize": "0.0050", "PRIME-ClearMode": "Pre-Exposure", "470 AO-MinVolts": "0.0000", "405 AO-SupportsTriggering": "1", "CameraChannelIndex": 0, "XYStage-StartSpeed": "500.0000", "COM9-StopBits": "2", "405 AO-Description": "NI DAC", "COM4-DelayBetweenCharsMs": "0.0000", "445 AO-Block voltage": "false", "520 AO-TriggerInputLine": "/Dev1/PFI0", "555 AO-Description": "NI DAC", "520 AO-TriggerSampleRate": "100000", "COM4-Verbose": "1", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM4-Name": "COM4", "405 Shutter-Volts": "5.0000", "Vincent-D3-Port": "COM1", "PRIME-PP 4 MIN ADU AFFECTED": "200", "470 Shutter-MinVolts": "0.0000", "PRIME-PP 3 ENABLED": "Yes", "470 AO-Description": "NI DAC", "PRIME-Description": "PVCAM API device adapter", "520 Shutter-MaxVolts": "5.0000", "520 Shutter-Volts": "0.0000", "528 AO-Sequenceable": "1", "PRIME-Binning": "1x1", "555 Shutter-MaxVolts": "5.0000", "528 Shutter-TriggerSequenceLength": "1000", "405 Shutter-Block voltage": "false", "XLIGHT Spin motor-State": "1", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "Vincent-D3-Time to open (ms)": "35.0000", "Vincent-D3-Time to close (ms)": "35.0000", "445 AO-TriggerSampleRate": "100000", "640 Shutter-IOChannel": "Dev1/ao14", "555 AO-Sequenceable": "1", "520 AO-Name": "AnalogIO", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "470 AO-Block voltage": "false", "COM1-Verbose": "1", "COM9-AnswerTimeout": "10000.0000", "528 Shutter-Name": "AnalogIO", "PRIME-Trigger Timeout (secs)": "10", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "NextFrame": 0, "470 AO-TriggerSequenceLength": "1000", "PRIME-TransposeXY": "0", "445 AO-TriggerSequenceLength": "1000", "COM1-Name": "COM1", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "COM9-DataBits": "8", "COM1-AnswerTimeout": "500.0000", "Camera": "", "TE2000-Version": "V2.50-V2.50-V2.10", "528 Shutter-Volts": "0.0000", "XLIGHT Hub-Name": "XLIGHT Hub", "PIEZO-Description": "Ludl stage driver adapter", "PositionName": "Pos29", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSampleRate": "100000", "PRIME-FanSpeedSetpoint": "High", "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "405 AO-TriggerSampleRate": "100000", "470 Shutter-Block voltage": "false", "470-State": "0", "COM3-Parity": "None", "405 AO-Volts": "4.5000", "PRIME-TransposeMirrorY": "0", "PRIME-ChipName": "GS144BSI", "470 AO-Volts": "0.0000", "PRIME-TransposeMirrorX": "0", "445 Shutter-SupportsTriggering": "0", "470 Shutter-SupportsTriggering": "0", "XLIGHT Hub-Description": "XLIGHT hub", "PRIME-PreampOffLimit": "10000", "470 AO-Sequenceable": "1", "528 Shutter-MaxVolts": "5.0000", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "Core-XYStage": "XYStage", "470 AO-TriggerSampleRate": "100000", "PRIME-Timing-PreTriggerDelayNs": "24672000.0000", "528-DA Device": "528 Shutter", "Focus-Description": "Nikon TE2000 focus adapter", "FilterBlock-State": "5", "520 AO-Demo": "No", "COM3-DelayBetweenCharsMs": "0.0000", "470 Shutter-Volts": "0.0000", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "PRIME-PP 2 THRESHOLD": "125", "PRIME-PP 4 THRESHOLD": "75", "XYStage-Acceleration": "4.0000", "470 Shutter-Demo": "No", "PRIME-Name": "PMPCIECam00", "640-DA Device": "640 Shutter", "405 AO-Block voltage": "false", "ElapsedTime-ms": 1226790, "PRIME-Offset": "100", "445 AO-IOChannel": "Dev1/ao1", "640 AO-MaxVolts": "5.0000", "PositionIndex": 29, "PRIME-PP 1 ENABLED": "Yes", "COM1-BaudRate": "9600", "640-State": "0", "COM3-Verbose": "1", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "LudlController-TransmissionDelay": "0", "PRIME-FullWellCapacity": "62000", "PRIME-SMARTStreamingEnabled": "No", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "640 Shutter-Description": "NI DAC", "528-Description": "DA device that is used as a shutter", "COM4-Description": "Serial port driver (boost:asio)", "405 Shutter-Description": "NI DAC", "TE2000-Name": "TE2000", "528 Shutter-Description": "NI DAC", "COM9-Description": "Serial port driver (boost:asio)", "LudlController-Name": "LudlController", "445 Shutter-TriggerSequenceLength": "1000", "PRIME-SerialNumber": "A18B203008", "445 AO-Volts": "0.0000", "PRIME-X-dimension": "1200", "PRIME-ShutterCloseDelay": "0", "XLIGHT Disk slider-State": "2", "Position": "Default", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 AO-IOChannel": "Dev1/ao5", "520 AO-IOChannel": "Dev1/ao4", "528 AO-TriggerSequenceLength": "1000", "405 Shutter-MaxVolts": "5.0000", "470-DA Device": "470 Shutter", "PRIME-FTCapable": "No", "Core-ChannelGroup": "Channels", "555 AO-SupportsTriggering": "0", "PRIME-Y-dimension": "1200", "Frame": 0, "555 Shutter-Sequenceable": "1", "PRIME-Timing-ExposureTimeNs": "499998000.0000", "Core-ImageProcessor": "", "528-State": "0", "520 AO-Block voltage": "false", "XLIGHT Excitation Wheel-Label": "QUAD", "640 Shutter-Demo": "No", "COM3-StopBits": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "520 Shutter-IOChannel": "Dev1/ao11", "Width": 1200, "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-SupportsTriggering": "0", "640 AO-TriggerSampleRate": "100000", "520-Description": "DA device that is used as a shutter", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "405 Shutter-Sequenceable": "1", "405 Shutter-SupportsTriggering": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-TriggerSampleRate": "100000", "640 Shutter-MaxVolts": "5.0000", "555 AO-MaxVolts": "5.0000", "520 AO-TriggerSequenceLength": "1000", "COM3-BaudRate": "9600", "COM9-BaudRate": "9600", "Vincent-D3-Channel #": "Ch. #1", "PRIME-PMode": "Normal", "OpticalPath-Label": "Left(100)", "BitDepth": 16, "555 AO-TriggerSequenceLength": "1000", "445 Shutter-Description": "NI DAC", "405 AO-Sequenceable": "1", "PRIME-AcquisitionMethod": "Callbacks", "555 AO-Name": "AnalogIO", "PRIME-ShutterMode": "Pre-Exposure", "COM3-Handshaking": "Off", "445 AO-SupportsTriggering": "0", "ROI": "0-0-1200-1200", "COM9-Verbose": "1", "PRIME-Color": "OFF", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "PRIME-ShutterOpenDelay": "0", "COM1-Parity": "None", "Slice": 8, "SlicePosition": 30, "COM9-DelayBetweenCharsMs": "0.0000", "555-Name": "DA Shutter", "PIEZO-LudlSingleAxisName": "B", "XLIGHT Emission Wheel-Label": "QUAD", "640 Shutter-Name": "AnalogIO", "640 AO-Sequenceable": "1", "520 Shutter-Demo": "No", "528 Shutter-Demo": "No", "470-Name": "DA Shutter", "520-DA Device": "520 Shutter", "XYStage-Description": "Ludl XY stage driver adapter", "445 Shutter-MinVolts": "0.0000", "528 AO-TriggerSampleRate": "100000", "405 AO-MinVolts": "0.0000", "405 Shutter-TriggerSequenceLength": "1000", "Height": 1200, "555 AO-Block voltage": "false", "640 Shutter-Volts": "0.0000", "PixelType": "GRAY16", "445 Shutter-Block voltage": "false", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "520 Shutter-TriggerSequenceLength": "1000", "COM9-Name": "COM9", "Core-SLM": "", "555 AO-Volts": "0.0000", "555 Shutter-Block voltage": "false", "XYStage-Speed": "1800.0000", "520 AO-MaxVolts": "5.0000", "528 Shutter-SupportsTriggering": "0", "COM1-Description": "Serial port driver (boost:asio)", "PRIME-MetadataEnabled": "No", "520 Shutter-MinVolts": "0.0000", "470 Shutter-Sequenceable": "1", "PRIME-PreampDelay": "0", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-MinVolts": "0.0000", "ChannelIndex": 5, "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Core-AutoShutter": "1", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "Exposure-ms": 300, "PRIME-PP 2 ENABLED": "Yes", "640 Shutter-TriggerSampleRate": "100000", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "470 Shutter-IOChannel": "Dev1/ao10", "405 AO-Demo": "No", "PixelSizeUm": 1.1, "470 AO-Demo": "No", "470 Shutter-MaxVolts": "5.0000", "Vincent-D3-Name": "Vincent-D3", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FileName": "img_000000000_P-DAPI-high_008.tif", "LudlController-Reset": "Operate", "PRIME-Exposure": "300.00", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "445-Name": "DA Shutter", "555 Shutter-Name": "AnalogIO", "520 Shutter-TriggerSampleRate": "100000", "445 Shutter-IOChannel": "Dev1/ao9", "PRIME-TransposeCorrection": "0", "XYStage-StepSize": "0.1000", "445-Description": "DA device that is used as a shutter", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 AO-IOChannel": "Dev1/ao7", "520 Shutter-Name": "AnalogIO", "COM3-DataBits": "8", "640 AO-Volts": "0.0000", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "555 Shutter-IOChannel": "Dev1/ao13", "WaitInterval": null, "520 Shutter-Sequenceable": "1", "YPositionUm": -180, "445-State": "0", "445-DA Device": "445 Shutter", "PRIME-CircularBufferAutoSize": "ON", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "555 AO-TriggerSampleRate": "100000", "445 Shutter-Volts": "0.0000", "XLIGHT Hub-Port": "COM3", "Core-AutoFocus": "", "COM4-Handshaking": "Off", "528 Shutter-MinVolts": "0.0000", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "445 Shutter-Name": "AnalogIO", "528 Shutter-Sequenceable": "1", "XYStage-TransposeMirrorX": "0", "XYStage-TransposeMirrorY": "0", "PRIME-ReadoutRate": "100MHz 16bit", "XYStage-Name": "Stage", "640 AO-SupportsTriggering": "0", "640-Name": "DA Shutter", "640 Shutter-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "640 AO-Block voltage": "false", "445 Shutter-Sequenceable": "1", "470 AO-MaxVolts": "5.0000", "COM4-BaudRate": "9600", "470-Description": "DA device that is used as a shutter", "520 Shutter-Description": "NI DAC", "PRIME-CCDTemperature": "-14.8500", "Time": "2019-12-18 23:11:26 -0500", "640 AO-TriggerSequenceLength": "1000", "470 AO-Name": "AnalogIO", "XLIGHT Disk slider-Label": "Disk Pos 40um", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "Binning": "1x1", "TE2000-ModelType": "1", "COM3-AnswerTimeout": "500.0000", "555 Shutter-SupportsTriggering": "0", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "TE2000-Port": "COM4", "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Description": "NI DAC", "COM4-AnswerTimeout": "500.0000", "COM1-DelayBetweenCharsMs": "0.0000", "FilterBlock-Label": "Confocal", "445 AO-Name": "AnalogIO", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-Timing-ReadoutTimeNs": "24672000.0000", "528 AO-Demo": "No", "520 AO-Sequenceable": "1", "555-Description": "DA device that is used as a shutter", "405-DA Device": "405 Shutter", "405 AO-TriggerSequenceLength": "1000", "640 AO-MinVolts": "0.0000", "640 Shutter-Block voltage": "false", "FrameIndex": 0, "PRIME-Port": "CMOS", "555 AO-MinVolts": "0.0000", "COM3-Description": "Serial port driver (boost:asio)", "528 AO-Description": "NI DAC", "555 AO-IOChannel": "Dev1/ao6", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "555 AO-TriggerInputLine": "/Dev1/PFI0", "PRIME-ActualInterval-ms": "390.5200", "LudlController-Port": "COM9", "445 Shutter-Demo": "No", "PRIME-Trigger-Expose Out-Mux": "1", "SliceIndex": 8, "PRIME-CCDTemperatureSetPoint": "-15.0000", "405 Shutter-MinVolts": "0.0000", "PRIME-ExposeOutMode": "First Row", "AxisPositions": null, "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Volts": "0.0000", "528-Name": "DA Shutter", "555-State": "0", "XLIGHT Dichroic-Label": "QUAD", "445 AO-Sequenceable": "1", "405-Name": "DA Shutter", "405 AO-IOChannel": "Dev1/ao0", "Core-Camera": "PRIME", "Focus-Name": "Focus", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "405 Shutter-IOChannel": "Dev1/ao8", "640 AO-Demo": "No", "640 Shutter-SupportsTriggering": "0", "445 AO-Demo": "No", "555-DA Device": "555 Shutter", "470 Shutter-Description": "NI DAC", "528 AO-Volts": "0.0000", "COM1-DataBits": "8", "Vincent-D3-Address": "x", "520 AO-SupportsTriggering": "0", "405-Description": "DA device that is used as a shutter", "640 Shutter-MinVolts": "0.0000", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "405 AO-Name": "AnalogIO", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "640 AO-TriggerInputLine": "/Dev1/PFI0", "405 AO-TriggerInputLine": "/Dev1/PFI0", "640-Description": "DA device that is used as a shutter", "445 Shutter-TriggerSampleRate": "100000", "470 Shutter-TriggerSequenceLength": "1000", "COM1-StopBits": "1", "520 Shutter-SupportsTriggering": "0", "405-State": "1", "Core-Initialize": "1", "XLIGHT Emission Wheel-State": "3", "520 AO-Description": "NI DAC", "528 AO-TriggerInputLine": "/Dev1/PFI0", "445 Shutter-MaxVolts": "5.0000", "COM9-Handshaking": "Off", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "XLIGHT Spin motor-Label": "On (spinning)", "PRIME-PP 5 ENABLED": "No", "UUID": "8c9a12d0-8eba-414c-ab8d-db33669ef9f3", "PRIME-TriggerMode": "Internal Trigger", "COM1-Handshaking": "Off", "PRIME-Timing-PostTriggerDelayNs": "49344000.0000", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "Focus-Position": "17741.5000", "528 Shutter-Block voltage": "false", "Channel": "P-DAPI-high", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "555 Shutter-Demo": "No", "405 Shutter-Demo": "No", "528 AO-Name": "AnalogIO", "520-State": "0", "Core-Galvo": "", "405 Shutter-TriggerSampleRate": "100000", "Focus-FocusStepSize_(nm)": "100", "PRIME-PixelType": "16bit", "COM4-DataBits": "8", "470 AO-IOChannel": "Dev1/ao2", "520 AO-MinVolts": "0.0000", "COM9-Parity": "None", "PRIME-CircularBufferEnabled": "ON", "PRIME-PP 6 Reset": "No", "640 AO-Description": "NI DAC", "Source": "PRIME", "445 AO-MinVolts": "0.0000", "PIEZO-Name": "Stage", "445 AO-Description": "NI DAC", "PRIME-CircularBufferFrameCount": "46", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "520 AO-Volts": "0.0000", "528 Shutter-TriggerSampleRate": "100000", "XPositionUm": 15712.1, "OpticalPath-State": "4", "PRIME-PP 3 THRESHOLD": "80", "PRIME-PVCAM Version": "3.7.1", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Gain": "1-HDR", "640 Shutter-TriggerSequenceLength": "1000", "528 AO-SupportsTriggering": "0", "445 AO-TriggerInputLine": "/Dev1/PFI0", "OpticalPath-Name": "OpticalPath", "PRIME-PP 4 ENABLED": "Yes", "PRIME-PVCAM Adapter Version": "1.3.29", "COM3-Name": "COM3", "405 AO-MaxVolts": "5.0000", "520 Shutter-Block voltage": "false", "PRIME-Timing-ClearingTimeNs": "49344000.0000", "Core-Focus": "PIEZO", "528 AO-Block voltage": "false", "TE2000-Description": "Nikon TE2000 hub", "528 AO-MinVolts": "0.0000", "COM4-Parity": "None", "Summary": { "Slices": 11, "ChContrastMin": [ 0, 0, 0, 0, 0, 0 ], "PixelAspect": 1, "SlicesFirst": true, "Interval_ms": 0, "Time": "2019-12-18 23:10:50 -0500", "Prefix": "seq_5", "InitialPositionList": [ { "Label": "Pos0", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.21], "Focus": [17741.5], "XYStage": [ 44542.200000000004, 31636.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos1", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.22], "Focus": [17741.4], "XYStage": [ 43801.100000000006, 31637.9 ] }, "GridColumnIndex": 0 }, { "Label": "Pos2", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.5], "XYStage": [ 38561.3, 32040.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos3", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.185], "Focus": [17741.3], "XYStage": [ 37698.4, 32041.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos4", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.16], "Focus": [17741.5], "XYStage": [ 32375.4, 31758.9 ] }, "GridColumnIndex": 0 }, { "Label": "Pos5", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.17000000000002], "Focus": [17741.3], "XYStage": [ 31770.7, 31758.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos6", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.4], "XYStage": [ 26536.800000000003, 31068.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos7", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.205], "Focus": [17741.4], "XYStage": [ 25420.2, 31068.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos8", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.85], "Focus": [17741.3], "XYStage": [ 21059, 31508 ] }, "GridColumnIndex": 0 }, { "Label": "Pos9", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.82], "Focus": [17741.3], "XYStage": [ 19731.9, 31506.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos10", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.79], "Focus": [17741.4], "XYStage": [ 14528.5, 32007.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos11", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.83], "Focus": [17741.2], "XYStage": [ 13830.300000000001, 32009.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos12", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.79], "Focus": [17741.3], "XYStage": [ 8775.300000000001, 32017.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos13", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.815], "Focus": [17741.3], "XYStage": [ 8109.200000000001, 32008.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos14", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.805], "Focus": [17741.3], "XYStage": [ 3483.3, 31908.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos15", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.82], "Focus": [17741.2], "XYStage": [ 2686.8, 31903.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos16", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [211.48000000000002], "Focus": [17741.1], "XYStage": [ -1978.9, 31973.600000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos17", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [211.51500000000001], "Focus": [17741.1], "XYStage": [ -2957.8, 31982.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos18", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.195], "Focus": [17741.5], "XYStage": [ 47747.4, -576.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos19", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.23000000000002], "Focus": [17741.6], "XYStage": [ 47053.9, -576.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos20", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.205], "Focus": [17741.4], "XYStage": [ 41871.200000000004, -479.8 ] }, "GridColumnIndex": 0 }, { "Label": "Pos21", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.4], "XYStage": [ 40882.600000000006, -392 ] }, "GridColumnIndex": 0 }, { "Label": "Pos22", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.24], "Focus": [17741.4], "XYStage": [ 35905.6, -135.3 ] }, "GridColumnIndex": 0 }, { "Label": "Pos23", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.195], "Focus": [17741.5], "XYStage": [ 34990.9, -132.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos24", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.46], "Focus": [17741.4], "XYStage": [ 29678.100000000002, -958.1 ] }, "GridColumnIndex": 0 }, { "Label": "Pos25", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.44], "Focus": [17741.5], "XYStage": [ 28983.600000000002, -939.4000000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos26", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.475], "Focus": [17741.5], "XYStage": [ 23019.7, -122 ] }, "GridColumnIndex": 0 }, { "Label": "Pos27", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.44], "Focus": [17741.7], "XYStage": [ 22428, -116.80000000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos28", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.46], "Focus": [17741.7], "XYStage": [ 16364.900000000001, -181.10000000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos29", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.48000000000002], "Focus": [17741.5], "XYStage": [ 15712.1, -180 ] }, "GridColumnIndex": 0 }, { "Label": "Pos30", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.45000000000002], "Focus": [17741.7], "XYStage": [ 10346.1, 77.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos31", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.49], "Focus": [17741.4], "XYStage": [ 9755.5, 85.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos32", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.475], "Focus": [17741.5], "XYStage": [ 4775.5, 293.90000000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos33", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.445], "Focus": [17741.4], "XYStage": [ 3954.9, 293.90000000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos34", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.455], "Focus": [17741.4], "XYStage": [ -1324.9, 545.6 ] }, "GridColumnIndex": 0 }, { "Label": "Pos35", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.445], "Focus": [17741.3], "XYStage": [ -2097, 576.6 ] }, "GridColumnIndex": 0 } ], "z-step_um": 10, "GridRow": 0, "BitDepth": 16, "Depth": 2, "Channels": 6, "Source": "Micro-Manager", "ChColors": [ -16711681, -256, -65281, -1, -1, -16776961 ], "MetadataVersion": 10, "KeepShutterOpenChannels": true, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536, 65536 ], "CustomIntervals_ms": [], "Height": 1200, "MicroManagerVersion": "1.4.23 20180219", "GridColumn": 0, "UUID": "67598d21-5db5-4842-ab60-f506f7b42c7c", "PixelType": "GRAY16", "Width": 1200, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\Yan\\20191218-YC113-S7S8-miseq", "Frames": 1, "Comment": "", "UserName": "Nikon", "PixelSize_um": 1.1, "ROI": [ 0, 0, 1200, 1200 ], "Date": "2019-12-18", "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC", "P-DAPI-high" ], "KeepShutterOpenSlices": true, "IJType": 1, "ComputerName": "ZadorConfocal2", "Positions": 36, "PositionIndex": 29, "CameraTimeout": 20000, "TimeFirst": false }, "640 AO-Name": "AnalogIO", "Vincent-D3-Command": "Close" }, "FrameKey-0-5-9": { "COM4-StopBits": "1", "528 AO-MaxVolts": "5.0000", "XLIGHT Excitation Wheel-State": "1", "470 Shutter-Name": "AnalogIO", "Core-Shutter": "405", "555 AO-Demo": "No", "XLIGHT Dichroic-State": "2", "XYStage-StepSize-Y": "0.1000", "ZPositionUm": 258.48, "XYStage-StepSize-X": "0.1000", "PRIME-FirmwareVersion": "20.28", "PRIME-ClearCycles": "2", "520-Name": "DA Shutter", "405 Shutter-Name": "AnalogIO", "PIEZO-StepSize": "0.0050", "PRIME-ClearMode": "Pre-Exposure", "470 AO-MinVolts": "0.0000", "405 AO-SupportsTriggering": "1", "CameraChannelIndex": 0, "XYStage-StartSpeed": "500.0000", "COM9-StopBits": "2", "405 AO-Description": "NI DAC", "COM4-DelayBetweenCharsMs": "0.0000", "445 AO-Block voltage": "false", "520 AO-TriggerInputLine": "/Dev1/PFI0", "555 AO-Description": "NI DAC", "520 AO-TriggerSampleRate": "100000", "COM4-Verbose": "1", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM4-Name": "COM4", "405 Shutter-Volts": "5.0000", "Vincent-D3-Port": "COM1", "PRIME-PP 4 MIN ADU AFFECTED": "200", "470 Shutter-MinVolts": "0.0000", "PRIME-PP 3 ENABLED": "Yes", "470 AO-Description": "NI DAC", "PRIME-Description": "PVCAM API device adapter", "520 Shutter-MaxVolts": "5.0000", "520 Shutter-Volts": "0.0000", "528 AO-Sequenceable": "1", "PRIME-Binning": "1x1", "555 Shutter-MaxVolts": "5.0000", "528 Shutter-TriggerSequenceLength": "1000", "405 Shutter-Block voltage": "false", "XLIGHT Spin motor-State": "1", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "Vincent-D3-Time to open (ms)": "35.0000", "Vincent-D3-Time to close (ms)": "35.0000", "445 AO-TriggerSampleRate": "100000", "640 Shutter-IOChannel": "Dev1/ao14", "555 AO-Sequenceable": "1", "520 AO-Name": "AnalogIO", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "470 AO-Block voltage": "false", "COM1-Verbose": "1", "COM9-AnswerTimeout": "10000.0000", "528 Shutter-Name": "AnalogIO", "PRIME-Trigger Timeout (secs)": "10", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "NextFrame": 0, "470 AO-TriggerSequenceLength": "1000", "PRIME-TransposeXY": "0", "445 AO-TriggerSequenceLength": "1000", "COM1-Name": "COM1", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "COM9-DataBits": "8", "COM1-AnswerTimeout": "500.0000", "Camera": "", "TE2000-Version": "V2.50-V2.50-V2.10", "528 Shutter-Volts": "0.0000", "XLIGHT Hub-Name": "XLIGHT Hub", "PIEZO-Description": "Ludl stage driver adapter", "PositionName": "Pos29", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSampleRate": "100000", "PRIME-FanSpeedSetpoint": "High", "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "405 AO-TriggerSampleRate": "100000", "470 Shutter-Block voltage": "false", "470-State": "0", "COM3-Parity": "None", "405 AO-Volts": "4.5000", "PRIME-TransposeMirrorY": "0", "PRIME-ChipName": "GS144BSI", "470 AO-Volts": "0.0000", "PRIME-TransposeMirrorX": "0", "445 Shutter-SupportsTriggering": "0", "470 Shutter-SupportsTriggering": "0", "XLIGHT Hub-Description": "XLIGHT hub", "PRIME-PreampOffLimit": "10000", "470 AO-Sequenceable": "1", "528 Shutter-MaxVolts": "5.0000", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "Core-XYStage": "XYStage", "470 AO-TriggerSampleRate": "100000", "PRIME-Timing-PreTriggerDelayNs": "24672000.0000", "528-DA Device": "528 Shutter", "Focus-Description": "Nikon TE2000 focus adapter", "FilterBlock-State": "5", "520 AO-Demo": "No", "COM3-DelayBetweenCharsMs": "0.0000", "470 Shutter-Volts": "0.0000", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "PRIME-PP 2 THRESHOLD": "125", "PRIME-PP 4 THRESHOLD": "75", "XYStage-Acceleration": "4.0000", "470 Shutter-Demo": "No", "PRIME-Name": "PMPCIECam00", "640-DA Device": "640 Shutter", "405 AO-Block voltage": "false", "ElapsedTime-ms": 1227345, "PRIME-Offset": "100", "445 AO-IOChannel": "Dev1/ao1", "640 AO-MaxVolts": "5.0000", "PositionIndex": 29, "PRIME-PP 1 ENABLED": "Yes", "COM1-BaudRate": "9600", "640-State": "0", "COM3-Verbose": "1", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "LudlController-TransmissionDelay": "0", "PRIME-FullWellCapacity": "62000", "PRIME-SMARTStreamingEnabled": "No", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "640 Shutter-Description": "NI DAC", "528-Description": "DA device that is used as a shutter", "COM4-Description": "Serial port driver (boost:asio)", "405 Shutter-Description": "NI DAC", "TE2000-Name": "TE2000", "528 Shutter-Description": "NI DAC", "COM9-Description": "Serial port driver (boost:asio)", "LudlController-Name": "LudlController", "445 Shutter-TriggerSequenceLength": "1000", "PRIME-SerialNumber": "A18B203008", "445 AO-Volts": "0.0000", "PRIME-X-dimension": "1200", "PRIME-ShutterCloseDelay": "0", "XLIGHT Disk slider-State": "2", "Position": "Default", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 AO-IOChannel": "Dev1/ao5", "520 AO-IOChannel": "Dev1/ao4", "528 AO-TriggerSequenceLength": "1000", "405 Shutter-MaxVolts": "5.0000", "470-DA Device": "470 Shutter", "PRIME-FTCapable": "No", "Core-ChannelGroup": "Channels", "555 AO-SupportsTriggering": "0", "PRIME-Y-dimension": "1200", "Frame": 0, "555 Shutter-Sequenceable": "1", "PRIME-Timing-ExposureTimeNs": "499998000.0000", "Core-ImageProcessor": "", "528-State": "0", "520 AO-Block voltage": "false", "XLIGHT Excitation Wheel-Label": "QUAD", "640 Shutter-Demo": "No", "COM3-StopBits": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "520 Shutter-IOChannel": "Dev1/ao11", "Width": 1200, "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-SupportsTriggering": "0", "640 AO-TriggerSampleRate": "100000", "520-Description": "DA device that is used as a shutter", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "405 Shutter-Sequenceable": "1", "405 Shutter-SupportsTriggering": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-TriggerSampleRate": "100000", "640 Shutter-MaxVolts": "5.0000", "555 AO-MaxVolts": "5.0000", "520 AO-TriggerSequenceLength": "1000", "COM3-BaudRate": "9600", "COM9-BaudRate": "9600", "Vincent-D3-Channel #": "Ch. #1", "PRIME-PMode": "Normal", "OpticalPath-Label": "Left(100)", "BitDepth": 16, "555 AO-TriggerSequenceLength": "1000", "445 Shutter-Description": "NI DAC", "405 AO-Sequenceable": "1", "PRIME-AcquisitionMethod": "Callbacks", "555 AO-Name": "AnalogIO", "PRIME-ShutterMode": "Pre-Exposure", "COM3-Handshaking": "Off", "445 AO-SupportsTriggering": "0", "ROI": "0-0-1200-1200", "COM9-Verbose": "1", "PRIME-Color": "OFF", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "PRIME-ShutterOpenDelay": "0", "COM1-Parity": "None", "Slice": 9, "SlicePosition": 40, "COM9-DelayBetweenCharsMs": "0.0000", "555-Name": "DA Shutter", "PIEZO-LudlSingleAxisName": "B", "XLIGHT Emission Wheel-Label": "QUAD", "640 Shutter-Name": "AnalogIO", "640 AO-Sequenceable": "1", "520 Shutter-Demo": "No", "528 Shutter-Demo": "No", "470-Name": "DA Shutter", "520-DA Device": "520 Shutter", "XYStage-Description": "Ludl XY stage driver adapter", "445 Shutter-MinVolts": "0.0000", "528 AO-TriggerSampleRate": "100000", "405 AO-MinVolts": "0.0000", "405 Shutter-TriggerSequenceLength": "1000", "Height": 1200, "555 AO-Block voltage": "false", "640 Shutter-Volts": "0.0000", "PixelType": "GRAY16", "445 Shutter-Block voltage": "false", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "520 Shutter-TriggerSequenceLength": "1000", "COM9-Name": "COM9", "Core-SLM": "", "555 AO-Volts": "0.0000", "555 Shutter-Block voltage": "false", "XYStage-Speed": "1800.0000", "520 AO-MaxVolts": "5.0000", "528 Shutter-SupportsTriggering": "0", "COM1-Description": "Serial port driver (boost:asio)", "PRIME-MetadataEnabled": "No", "520 Shutter-MinVolts": "0.0000", "470 Shutter-Sequenceable": "1", "PRIME-PreampDelay": "0", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-MinVolts": "0.0000", "ChannelIndex": 5, "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Core-AutoShutter": "1", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "Exposure-ms": 300, "PRIME-PP 2 ENABLED": "Yes", "640 Shutter-TriggerSampleRate": "100000", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "470 Shutter-IOChannel": "Dev1/ao10", "405 AO-Demo": "No", "PixelSizeUm": 1.1, "470 AO-Demo": "No", "470 Shutter-MaxVolts": "5.0000", "Vincent-D3-Name": "Vincent-D3", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FileName": "img_000000000_P-DAPI-high_009.tif", "LudlController-Reset": "Operate", "PRIME-Exposure": "300.00", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "445-Name": "DA Shutter", "555 Shutter-Name": "AnalogIO", "520 Shutter-TriggerSampleRate": "100000", "445 Shutter-IOChannel": "Dev1/ao9", "PRIME-TransposeCorrection": "0", "XYStage-StepSize": "0.1000", "445-Description": "DA device that is used as a shutter", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 AO-IOChannel": "Dev1/ao7", "520 Shutter-Name": "AnalogIO", "COM3-DataBits": "8", "640 AO-Volts": "0.0000", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "555 Shutter-IOChannel": "Dev1/ao13", "WaitInterval": null, "520 Shutter-Sequenceable": "1", "YPositionUm": -180, "445-State": "0", "445-DA Device": "445 Shutter", "PRIME-CircularBufferAutoSize": "ON", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "555 AO-TriggerSampleRate": "100000", "445 Shutter-Volts": "0.0000", "XLIGHT Hub-Port": "COM3", "Core-AutoFocus": "", "COM4-Handshaking": "Off", "528 Shutter-MinVolts": "0.0000", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "445 Shutter-Name": "AnalogIO", "528 Shutter-Sequenceable": "1", "XYStage-TransposeMirrorX": "0", "XYStage-TransposeMirrorY": "0", "PRIME-ReadoutRate": "100MHz 16bit", "XYStage-Name": "Stage", "640 AO-SupportsTriggering": "0", "640-Name": "DA Shutter", "640 Shutter-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "640 AO-Block voltage": "false", "445 Shutter-Sequenceable": "1", "470 AO-MaxVolts": "5.0000", "COM4-BaudRate": "9600", "470-Description": "DA device that is used as a shutter", "520 Shutter-Description": "NI DAC", "PRIME-CCDTemperature": "-14.8500", "Time": "2019-12-18 23:11:27 -0500", "640 AO-TriggerSequenceLength": "1000", "470 AO-Name": "AnalogIO", "XLIGHT Disk slider-Label": "Disk Pos 40um", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "Binning": "1x1", "TE2000-ModelType": "1", "COM3-AnswerTimeout": "500.0000", "555 Shutter-SupportsTriggering": "0", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "TE2000-Port": "COM4", "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Description": "NI DAC", "COM4-AnswerTimeout": "500.0000", "COM1-DelayBetweenCharsMs": "0.0000", "FilterBlock-Label": "Confocal", "445 AO-Name": "AnalogIO", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-Timing-ReadoutTimeNs": "24672000.0000", "528 AO-Demo": "No", "520 AO-Sequenceable": "1", "555-Description": "DA device that is used as a shutter", "405-DA Device": "405 Shutter", "405 AO-TriggerSequenceLength": "1000", "640 AO-MinVolts": "0.0000", "640 Shutter-Block voltage": "false", "FrameIndex": 0, "PRIME-Port": "CMOS", "555 AO-MinVolts": "0.0000", "COM3-Description": "Serial port driver (boost:asio)", "528 AO-Description": "NI DAC", "555 AO-IOChannel": "Dev1/ao6", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "555 AO-TriggerInputLine": "/Dev1/PFI0", "PRIME-ActualInterval-ms": "390.5200", "LudlController-Port": "COM9", "445 Shutter-Demo": "No", "PRIME-Trigger-Expose Out-Mux": "1", "SliceIndex": 9, "PRIME-CCDTemperatureSetPoint": "-15.0000", "405 Shutter-MinVolts": "0.0000", "PRIME-ExposeOutMode": "First Row", "AxisPositions": null, "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Volts": "0.0000", "528-Name": "DA Shutter", "555-State": "0", "XLIGHT Dichroic-Label": "QUAD", "445 AO-Sequenceable": "1", "405-Name": "DA Shutter", "405 AO-IOChannel": "Dev1/ao0", "Core-Camera": "PRIME", "Focus-Name": "Focus", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "405 Shutter-IOChannel": "Dev1/ao8", "640 AO-Demo": "No", "640 Shutter-SupportsTriggering": "0", "445 AO-Demo": "No", "555-DA Device": "555 Shutter", "470 Shutter-Description": "NI DAC", "528 AO-Volts": "0.0000", "COM1-DataBits": "8", "Vincent-D3-Address": "x", "520 AO-SupportsTriggering": "0", "405-Description": "DA device that is used as a shutter", "640 Shutter-MinVolts": "0.0000", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "405 AO-Name": "AnalogIO", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "640 AO-TriggerInputLine": "/Dev1/PFI0", "405 AO-TriggerInputLine": "/Dev1/PFI0", "640-Description": "DA device that is used as a shutter", "445 Shutter-TriggerSampleRate": "100000", "470 Shutter-TriggerSequenceLength": "1000", "COM1-StopBits": "1", "520 Shutter-SupportsTriggering": "0", "405-State": "1", "Core-Initialize": "1", "XLIGHT Emission Wheel-State": "3", "520 AO-Description": "NI DAC", "528 AO-TriggerInputLine": "/Dev1/PFI0", "445 Shutter-MaxVolts": "5.0000", "COM9-Handshaking": "Off", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "XLIGHT Spin motor-Label": "On (spinning)", "PRIME-PP 5 ENABLED": "No", "UUID": "9143ee20-8b0e-4e37-93dc-6b49c2e3a165", "PRIME-TriggerMode": "Internal Trigger", "COM1-Handshaking": "Off", "PRIME-Timing-PostTriggerDelayNs": "49344000.0000", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "Focus-Position": "17741.5000", "528 Shutter-Block voltage": "false", "Channel": "P-DAPI-high", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "555 Shutter-Demo": "No", "405 Shutter-Demo": "No", "528 AO-Name": "AnalogIO", "520-State": "0", "Core-Galvo": "", "405 Shutter-TriggerSampleRate": "100000", "Focus-FocusStepSize_(nm)": "100", "PRIME-PixelType": "16bit", "COM4-DataBits": "8", "470 AO-IOChannel": "Dev1/ao2", "520 AO-MinVolts": "0.0000", "COM9-Parity": "None", "PRIME-CircularBufferEnabled": "ON", "PRIME-PP 6 Reset": "No", "640 AO-Description": "NI DAC", "Source": "PRIME", "445 AO-MinVolts": "0.0000", "PIEZO-Name": "Stage", "445 AO-Description": "NI DAC", "PRIME-CircularBufferFrameCount": "46", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "520 AO-Volts": "0.0000", "528 Shutter-TriggerSampleRate": "100000", "XPositionUm": 15712.1, "OpticalPath-State": "4", "PRIME-PP 3 THRESHOLD": "80", "PRIME-PVCAM Version": "3.7.1", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Gain": "1-HDR", "640 Shutter-TriggerSequenceLength": "1000", "528 AO-SupportsTriggering": "0", "445 AO-TriggerInputLine": "/Dev1/PFI0", "OpticalPath-Name": "OpticalPath", "PRIME-PP 4 ENABLED": "Yes", "PRIME-PVCAM Adapter Version": "1.3.29", "COM3-Name": "COM3", "405 AO-MaxVolts": "5.0000", "520 Shutter-Block voltage": "false", "PRIME-Timing-ClearingTimeNs": "49344000.0000", "Core-Focus": "PIEZO", "528 AO-Block voltage": "false", "TE2000-Description": "Nikon TE2000 hub", "528 AO-MinVolts": "0.0000", "COM4-Parity": "None", "Summary": { "Slices": 11, "ChContrastMin": [ 0, 0, 0, 0, 0, 0 ], "PixelAspect": 1, "SlicesFirst": true, "Interval_ms": 0, "Time": "2019-12-18 23:10:50 -0500", "Prefix": "seq_5", "InitialPositionList": [ { "Label": "Pos0", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.21], "Focus": [17741.5], "XYStage": [ 44542.200000000004, 31636.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos1", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.22], "Focus": [17741.4], "XYStage": [ 43801.100000000006, 31637.9 ] }, "GridColumnIndex": 0 }, { "Label": "Pos2", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.5], "XYStage": [ 38561.3, 32040.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos3", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.185], "Focus": [17741.3], "XYStage": [ 37698.4, 32041.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos4", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.16], "Focus": [17741.5], "XYStage": [ 32375.4, 31758.9 ] }, "GridColumnIndex": 0 }, { "Label": "Pos5", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.17000000000002], "Focus": [17741.3], "XYStage": [ 31770.7, 31758.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos6", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.4], "XYStage": [ 26536.800000000003, 31068.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos7", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.205], "Focus": [17741.4], "XYStage": [ 25420.2, 31068.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos8", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.85], "Focus": [17741.3], "XYStage": [ 21059, 31508 ] }, "GridColumnIndex": 0 }, { "Label": "Pos9", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.82], "Focus": [17741.3], "XYStage": [ 19731.9, 31506.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos10", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.79], "Focus": [17741.4], "XYStage": [ 14528.5, 32007.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos11", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.83], "Focus": [17741.2], "XYStage": [ 13830.300000000001, 32009.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos12", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.79], "Focus": [17741.3], "XYStage": [ 8775.300000000001, 32017.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos13", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.815], "Focus": [17741.3], "XYStage": [ 8109.200000000001, 32008.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos14", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.805], "Focus": [17741.3], "XYStage": [ 3483.3, 31908.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos15", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.82], "Focus": [17741.2], "XYStage": [ 2686.8, 31903.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos16", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [211.48000000000002], "Focus": [17741.1], "XYStage": [ -1978.9, 31973.600000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos17", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [211.51500000000001], "Focus": [17741.1], "XYStage": [ -2957.8, 31982.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos18", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.195], "Focus": [17741.5], "XYStage": [ 47747.4, -576.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos19", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.23000000000002], "Focus": [17741.6], "XYStage": [ 47053.9, -576.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos20", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.205], "Focus": [17741.4], "XYStage": [ 41871.200000000004, -479.8 ] }, "GridColumnIndex": 0 }, { "Label": "Pos21", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.4], "XYStage": [ 40882.600000000006, -392 ] }, "GridColumnIndex": 0 }, { "Label": "Pos22", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.24], "Focus": [17741.4], "XYStage": [ 35905.6, -135.3 ] }, "GridColumnIndex": 0 }, { "Label": "Pos23", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.195], "Focus": [17741.5], "XYStage": [ 34990.9, -132.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos24", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.46], "Focus": [17741.4], "XYStage": [ 29678.100000000002, -958.1 ] }, "GridColumnIndex": 0 }, { "Label": "Pos25", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.44], "Focus": [17741.5], "XYStage": [ 28983.600000000002, -939.4000000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos26", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.475], "Focus": [17741.5], "XYStage": [ 23019.7, -122 ] }, "GridColumnIndex": 0 }, { "Label": "Pos27", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.44], "Focus": [17741.7], "XYStage": [ 22428, -116.80000000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos28", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.46], "Focus": [17741.7], "XYStage": [ 16364.900000000001, -181.10000000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos29", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.48000000000002], "Focus": [17741.5], "XYStage": [ 15712.1, -180 ] }, "GridColumnIndex": 0 }, { "Label": "Pos30", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.45000000000002], "Focus": [17741.7], "XYStage": [ 10346.1, 77.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos31", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.49], "Focus": [17741.4], "XYStage": [ 9755.5, 85.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos32", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.475], "Focus": [17741.5], "XYStage": [ 4775.5, 293.90000000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos33", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.445], "Focus": [17741.4], "XYStage": [ 3954.9, 293.90000000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos34", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.455], "Focus": [17741.4], "XYStage": [ -1324.9, 545.6 ] }, "GridColumnIndex": 0 }, { "Label": "Pos35", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.445], "Focus": [17741.3], "XYStage": [ -2097, 576.6 ] }, "GridColumnIndex": 0 } ], "z-step_um": 10, "GridRow": 0, "BitDepth": 16, "Depth": 2, "Channels": 6, "Source": "Micro-Manager", "ChColors": [ -16711681, -256, -65281, -1, -1, -16776961 ], "MetadataVersion": 10, "KeepShutterOpenChannels": true, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536, 65536 ], "CustomIntervals_ms": [], "Height": 1200, "MicroManagerVersion": "1.4.23 20180219", "GridColumn": 0, "UUID": "67598d21-5db5-4842-ab60-f506f7b42c7c", "PixelType": "GRAY16", "Width": 1200, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\Yan\\20191218-YC113-S7S8-miseq", "Frames": 1, "Comment": "", "UserName": "Nikon", "PixelSize_um": 1.1, "ROI": [ 0, 0, 1200, 1200 ], "Date": "2019-12-18", "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC", "P-DAPI-high" ], "KeepShutterOpenSlices": true, "IJType": 1, "ComputerName": "ZadorConfocal2", "Positions": 36, "PositionIndex": 29, "CameraTimeout": 20000, "TimeFirst": false }, "640 AO-Name": "AnalogIO", "Vincent-D3-Command": "Close" }, "FrameKey-0-5-10": { "COM4-StopBits": "1", "528 AO-MaxVolts": "5.0000", "XLIGHT Excitation Wheel-State": "1", "470 Shutter-Name": "AnalogIO", "Core-Shutter": "405", "555 AO-Demo": "No", "XLIGHT Dichroic-State": "2", "XYStage-StepSize-Y": "0.1000", "ZPositionUm": 268.48, "XYStage-StepSize-X": "0.1000", "PRIME-FirmwareVersion": "20.28", "PRIME-ClearCycles": "2", "520-Name": "DA Shutter", "405 Shutter-Name": "AnalogIO", "PIEZO-StepSize": "0.0050", "PRIME-ClearMode": "Pre-Exposure", "470 AO-MinVolts": "0.0000", "405 AO-SupportsTriggering": "1", "CameraChannelIndex": 0, "XYStage-StartSpeed": "500.0000", "COM9-StopBits": "2", "405 AO-Description": "NI DAC", "COM4-DelayBetweenCharsMs": "0.0000", "445 AO-Block voltage": "false", "520 AO-TriggerInputLine": "/Dev1/PFI0", "555 AO-Description": "NI DAC", "520 AO-TriggerSampleRate": "100000", "COM4-Verbose": "1", "Vincent-D3-Description": "Vincent D3 controller adapter", "520 Shutter-TriggerInputLine": "/Dev1/PFI0", "COM4-Name": "COM4", "405 Shutter-Volts": "5.0000", "Vincent-D3-Port": "COM1", "PRIME-PP 4 MIN ADU AFFECTED": "200", "470 Shutter-MinVolts": "0.0000", "PRIME-PP 3 ENABLED": "Yes", "470 AO-Description": "NI DAC", "PRIME-Description": "PVCAM API device adapter", "520 Shutter-MaxVolts": "5.0000", "520 Shutter-Volts": "0.0000", "528 AO-Sequenceable": "1", "PRIME-Binning": "1x1", "555 Shutter-MaxVolts": "5.0000", "528 Shutter-TriggerSequenceLength": "1000", "405 Shutter-Block voltage": "false", "XLIGHT Spin motor-State": "1", "520 AO-AutoDetectedOutputPort": "Dev1/ao4", "Vincent-D3-Time to open (ms)": "35.0000", "Vincent-D3-Time to close (ms)": "35.0000", "445 AO-TriggerSampleRate": "100000", "640 Shutter-IOChannel": "Dev1/ao14", "555 AO-Sequenceable": "1", "520 AO-Name": "AnalogIO", "520 Shutter-AutoDetectedOutputPort": "Dev1/ao11", "470 AO-Block voltage": "false", "COM1-Verbose": "1", "COM9-AnswerTimeout": "10000.0000", "528 Shutter-Name": "AnalogIO", "PRIME-Trigger Timeout (secs)": "10", "LudlController-Version": "Version no. : 13.500 MAC6000 Interface with USB/ETH ", "NextFrame": 0, "470 AO-TriggerSequenceLength": "1000", "PRIME-TransposeXY": "0", "445 AO-TriggerSequenceLength": "1000", "COM1-Name": "COM1", "XLIGHT Emission Wheel-Name": "XLIGHT Emission Wheel", "PRIME-PP 4 QUANTVIEW": "QUANTVIEW", "640 AO-AutoDetectedOutputPort": "Dev1/ao7", "COM9-DataBits": "8", "COM1-AnswerTimeout": "500.0000", "Camera": "", "TE2000-Version": "V2.50-V2.50-V2.10", "528 Shutter-Volts": "0.0000", "XLIGHT Hub-Name": "XLIGHT Hub", "PIEZO-Description": "Ludl stage driver adapter", "PositionName": "Pos29", "Core-TimeoutMs": "5000", "470 Shutter-TriggerSampleRate": "100000", "PRIME-FanSpeedSetpoint": "High", "FilterBlock-Description": "Nikon TE2000 filter block changeover adapter", "405 AO-TriggerSampleRate": "100000", "470 Shutter-Block voltage": "false", "470-State": "0", "COM3-Parity": "None", "405 AO-Volts": "4.5000", "PRIME-TransposeMirrorY": "0", "PRIME-ChipName": "GS144BSI", "470 AO-Volts": "0.0000", "PRIME-TransposeMirrorX": "0", "445 Shutter-SupportsTriggering": "0", "470 Shutter-SupportsTriggering": "0", "XLIGHT Hub-Description": "XLIGHT hub", "PRIME-PreampOffLimit": "10000", "470 AO-Sequenceable": "1", "528 Shutter-MaxVolts": "5.0000", "445 Shutter-AutoDetectedOutputPort": "Dev1/ao9", "FilterBlock-Name": "FilterBlock", "PRIME-CircularBufferFrameRecovery": "OFF", "Core-XYStage": "XYStage", "470 AO-TriggerSampleRate": "100000", "PRIME-Timing-PreTriggerDelayNs": "24672000.0000", "528-DA Device": "528 Shutter", "Focus-Description": "Nikon TE2000 focus adapter", "FilterBlock-State": "5", "520 AO-Demo": "No", "COM3-DelayBetweenCharsMs": "0.0000", "470 Shutter-Volts": "0.0000", "528 AO-AutoDetectedOutputPort": "Dev1/ao5", "PRIME-PP 2 THRESHOLD": "125", "PRIME-PP 4 THRESHOLD": "75", "XYStage-Acceleration": "4.0000", "470 Shutter-Demo": "No", "PRIME-Name": "PMPCIECam00", "640-DA Device": "640 Shutter", "405 AO-Block voltage": "false", "ElapsedTime-ms": 1227881, "PRIME-Offset": "100", "445 AO-IOChannel": "Dev1/ao1", "640 AO-MaxVolts": "5.0000", "PositionIndex": 29, "PRIME-PP 1 ENABLED": "Yes", "COM1-BaudRate": "9600", "640-State": "0", "COM3-Verbose": "1", "405 AO-AutoDetectedOutputPort": "Dev1/ao0", "LudlController-TransmissionDelay": "0", "PRIME-FullWellCapacity": "62000", "PRIME-SMARTStreamingEnabled": "No", "555 AO-AutoDetectedOutputPort": "Dev1/ao6", "640 Shutter-Description": "NI DAC", "528-Description": "DA device that is used as a shutter", "COM4-Description": "Serial port driver (boost:asio)", "405 Shutter-Description": "NI DAC", "TE2000-Name": "TE2000", "528 Shutter-Description": "NI DAC", "COM9-Description": "Serial port driver (boost:asio)", "LudlController-Name": "LudlController", "445 Shutter-TriggerSequenceLength": "1000", "PRIME-SerialNumber": "A18B203008", "445 AO-Volts": "0.0000", "PRIME-X-dimension": "1200", "PRIME-ShutterCloseDelay": "0", "XLIGHT Disk slider-State": "2", "Position": "Default", "470 Shutter-TriggerInputLine": "/Dev1/PFI0", "528 AO-IOChannel": "Dev1/ao5", "520 AO-IOChannel": "Dev1/ao4", "528 AO-TriggerSequenceLength": "1000", "405 Shutter-MaxVolts": "5.0000", "470-DA Device": "470 Shutter", "PRIME-FTCapable": "No", "Core-ChannelGroup": "Channels", "555 AO-SupportsTriggering": "0", "PRIME-Y-dimension": "1200", "Frame": 0, "555 Shutter-Sequenceable": "1", "PRIME-Timing-ExposureTimeNs": "499998000.0000", "Core-ImageProcessor": "", "528-State": "0", "520 AO-Block voltage": "false", "XLIGHT Excitation Wheel-Label": "QUAD", "640 Shutter-Demo": "No", "COM3-StopBits": "1", "PRIME-SMARTStreamingValues[ms]": "10.000;20.000;30.000;40.000", "XLIGHT Dichroic-Description": "XLIGHT Dichroic filter", "520 Shutter-IOChannel": "Dev1/ao11", "Width": 1200, "640 Shutter-AutoDetectedOutputPort": "Dev1/ao14", "470 AO-SupportsTriggering": "0", "640 AO-TriggerSampleRate": "100000", "520-Description": "DA device that is used as a shutter", "XLIGHT Spin motor-Name": "XLIGHT Spin motor", "405 Shutter-Sequenceable": "1", "405 Shutter-SupportsTriggering": "0", "445 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-TriggerSampleRate": "100000", "640 Shutter-MaxVolts": "5.0000", "555 AO-MaxVolts": "5.0000", "520 AO-TriggerSequenceLength": "1000", "COM3-BaudRate": "9600", "COM9-BaudRate": "9600", "Vincent-D3-Channel #": "Ch. #1", "PRIME-PMode": "Normal", "OpticalPath-Label": "Left(100)", "BitDepth": 16, "555 AO-TriggerSequenceLength": "1000", "445 Shutter-Description": "NI DAC", "405 AO-Sequenceable": "1", "PRIME-AcquisitionMethod": "Callbacks", "555 AO-Name": "AnalogIO", "PRIME-ShutterMode": "Pre-Exposure", "COM3-Handshaking": "Off", "445 AO-SupportsTriggering": "0", "ROI": "0-0-1200-1200", "COM9-Verbose": "1", "PRIME-Color": "OFF", "555 Shutter-AutoDetectedOutputPort": "Dev1/ao13", "PRIME-ShutterOpenDelay": "0", "COM1-Parity": "None", "Slice": 10, "SlicePosition": 50, "COM9-DelayBetweenCharsMs": "0.0000", "555-Name": "DA Shutter", "PIEZO-LudlSingleAxisName": "B", "XLIGHT Emission Wheel-Label": "QUAD", "640 Shutter-Name": "AnalogIO", "640 AO-Sequenceable": "1", "520 Shutter-Demo": "No", "528 Shutter-Demo": "No", "470-Name": "DA Shutter", "520-DA Device": "520 Shutter", "XYStage-Description": "Ludl XY stage driver adapter", "445 Shutter-MinVolts": "0.0000", "528 AO-TriggerSampleRate": "100000", "405 AO-MinVolts": "0.0000", "405 Shutter-TriggerSequenceLength": "1000", "Height": 1200, "555 AO-Block voltage": "false", "640 Shutter-Volts": "0.0000", "PixelType": "GRAY16", "445 Shutter-Block voltage": "false", "445 AO-MaxVolts": "5.0000", "PRIME-PP 1 THRESHOLD": "125", "520 Shutter-TriggerSequenceLength": "1000", "COM9-Name": "COM9", "Core-SLM": "", "555 AO-Volts": "0.0000", "555 Shutter-Block voltage": "false", "XYStage-Speed": "1800.0000", "520 AO-MaxVolts": "5.0000", "528 Shutter-SupportsTriggering": "0", "COM1-Description": "Serial port driver (boost:asio)", "PRIME-MetadataEnabled": "No", "520 Shutter-MinVolts": "0.0000", "470 Shutter-Sequenceable": "1", "PRIME-PreampDelay": "0", "528 Shutter-IOChannel": "Dev1/ao12", "555 Shutter-MinVolts": "0.0000", "ChannelIndex": 5, "PRIME-PP 3 DESPECKLE DARK HIGH": "DESPECKLE DARK HIGH", "Core-AutoShutter": "1", "XLIGHT Excitation Wheel-Name": "XLIGHT Excitation Wheel", "Exposure-ms": 300, "PRIME-PP 2 ENABLED": "Yes", "640 Shutter-TriggerSampleRate": "100000", "XLIGHT Disk slider-Name": "XLIGHT Disk slider", "470 Shutter-IOChannel": "Dev1/ao10", "405 AO-Demo": "No", "PixelSizeUm": 1.1, "470 AO-Demo": "No", "470 Shutter-MaxVolts": "5.0000", "Vincent-D3-Name": "Vincent-D3", "PRIME-PP 2 DESPECKLE DARK LOW": "DESPECKLE DARK LOW", "FileName": "img_000000000_P-DAPI-high_010.tif", "LudlController-Reset": "Operate", "PRIME-Exposure": "300.00", "XLIGHT Emission Wheel-Description": "XLIGHT Emission Wheel Position", "445-Name": "DA Shutter", "555 Shutter-Name": "AnalogIO", "520 Shutter-TriggerSampleRate": "100000", "445 Shutter-IOChannel": "Dev1/ao9", "PRIME-TransposeCorrection": "0", "XYStage-StepSize": "0.1000", "445-Description": "DA device that is used as a shutter", "XLIGHT Excitation Wheel-Description": "XLIGHT Excitation Wheel Position", "640 AO-IOChannel": "Dev1/ao7", "520 Shutter-Name": "AnalogIO", "COM3-DataBits": "8", "640 AO-Volts": "0.0000", "470 AO-AutoDetectedOutputPort": "Dev1/ao2", "555 Shutter-IOChannel": "Dev1/ao13", "WaitInterval": null, "520 Shutter-Sequenceable": "1", "YPositionUm": -180, "445-State": "0", "445-DA Device": "445 Shutter", "PRIME-CircularBufferAutoSize": "ON", "OpticalPath-Description": "Nikon TE2000 optical path adapter", "555 AO-TriggerSampleRate": "100000", "445 Shutter-Volts": "0.0000", "XLIGHT Hub-Port": "COM3", "Core-AutoFocus": "", "COM4-Handshaking": "Off", "528 Shutter-MinVolts": "0.0000", "XLIGHT Dichroic-Name": "XLIGHT Dichroic", "445 Shutter-Name": "AnalogIO", "528 Shutter-Sequenceable": "1", "XYStage-TransposeMirrorX": "0", "XYStage-TransposeMirrorY": "0", "PRIME-ReadoutRate": "100MHz 16bit", "XYStage-Name": "Stage", "640 AO-SupportsTriggering": "0", "640-Name": "DA Shutter", "640 Shutter-Sequenceable": "1", "PRIME-PP 2 MIN ADU AFFECTED": "200", "640 AO-Block voltage": "false", "445 Shutter-Sequenceable": "1", "470 AO-MaxVolts": "5.0000", "COM4-BaudRate": "9600", "470-Description": "DA device that is used as a shutter", "520 Shutter-Description": "NI DAC", "PRIME-CCDTemperature": "-14.8500", "Time": "2019-12-18 23:11:28 -0500", "640 AO-TriggerSequenceLength": "1000", "470 AO-Name": "AnalogIO", "XLIGHT Disk slider-Label": "Disk Pos 40um", "405 Shutter-TriggerInputLine": "/Dev1/PFI0", "Binning": "1x1", "TE2000-ModelType": "1", "COM3-AnswerTimeout": "500.0000", "555 Shutter-SupportsTriggering": "0", "XLIGHT Disk slider-Description": "XLIGHT Disk slider", "TE2000-Port": "COM4", "LudlController-Description": "Ludl Controller (Mac 2000/5000)", "470 AO-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Description": "NI DAC", "COM4-AnswerTimeout": "500.0000", "COM1-DelayBetweenCharsMs": "0.0000", "FilterBlock-Label": "Confocal", "445 AO-Name": "AnalogIO", "640 Shutter-TriggerInputLine": "/Dev1/PFI0", "PRIME-Timing-ReadoutTimeNs": "24672000.0000", "528 AO-Demo": "No", "520 AO-Sequenceable": "1", "555-Description": "DA device that is used as a shutter", "405-DA Device": "405 Shutter", "405 AO-TriggerSequenceLength": "1000", "640 AO-MinVolts": "0.0000", "640 Shutter-Block voltage": "false", "FrameIndex": 0, "PRIME-Port": "CMOS", "555 AO-MinVolts": "0.0000", "COM3-Description": "Serial port driver (boost:asio)", "528 AO-Description": "NI DAC", "555 AO-IOChannel": "Dev1/ao6", "528 Shutter-AutoDetectedOutputPort": "Dev1/ao12", "555 AO-TriggerInputLine": "/Dev1/PFI0", "PRIME-ActualInterval-ms": "390.5200", "LudlController-Port": "COM9", "445 Shutter-Demo": "No", "PRIME-Trigger-Expose Out-Mux": "1", "SliceIndex": 10, "PRIME-CCDTemperatureSetPoint": "-15.0000", "405 Shutter-MinVolts": "0.0000", "PRIME-ExposeOutMode": "First Row", "AxisPositions": null, "555 Shutter-TriggerInputLine": "/Dev1/PFI0", "555 Shutter-Volts": "0.0000", "528-Name": "DA Shutter", "555-State": "0", "XLIGHT Dichroic-Label": "QUAD", "445 AO-Sequenceable": "1", "405-Name": "DA Shutter", "405 AO-IOChannel": "Dev1/ao0", "Core-Camera": "PRIME", "Focus-Name": "Focus", "445 AO-AutoDetectedOutputPort": "Dev1/ao1", "405 Shutter-IOChannel": "Dev1/ao8", "640 AO-Demo": "No", "640 Shutter-SupportsTriggering": "0", "445 AO-Demo": "No", "555-DA Device": "555 Shutter", "470 Shutter-Description": "NI DAC", "528 AO-Volts": "0.0000", "COM1-DataBits": "8", "Vincent-D3-Address": "x", "520 AO-SupportsTriggering": "0", "405-Description": "DA device that is used as a shutter", "640 Shutter-MinVolts": "0.0000", "470 Shutter-AutoDetectedOutputPort": "Dev1/ao10", "405 AO-Name": "AnalogIO", "PRIME-PP 0 DESPECKLE BRIGHT LOW": "DESPECKLE BRIGHT LOW", "640 AO-TriggerInputLine": "/Dev1/PFI0", "405 AO-TriggerInputLine": "/Dev1/PFI0", "640-Description": "DA device that is used as a shutter", "445 Shutter-TriggerSampleRate": "100000", "470 Shutter-TriggerSequenceLength": "1000", "COM1-StopBits": "1", "520 Shutter-SupportsTriggering": "0", "405-State": "0", "Core-Initialize": "1", "XLIGHT Emission Wheel-State": "3", "520 AO-Description": "NI DAC", "528 AO-TriggerInputLine": "/Dev1/PFI0", "445 Shutter-MaxVolts": "5.0000", "COM9-Handshaking": "Off", "528 Shutter-TriggerInputLine": "/Dev1/PFI0", "XLIGHT Spin motor-Label": "On (spinning)", "PRIME-PP 5 ENABLED": "No", "UUID": "25ea09ee-0798-47e9-b2ea-e9810f1c923c", "PRIME-TriggerMode": "Internal Trigger", "COM1-Handshaking": "Off", "PRIME-Timing-PostTriggerDelayNs": "49344000.0000", "XLIGHT Spin motor-Description": "XLIGHT Disk spin motor", "Focus-Position": "17741.5000", "528 Shutter-Block voltage": "false", "Channel": "P-DAPI-high", "405 Shutter-AutoDetectedOutputPort": "Dev1/ao8", "555 Shutter-Demo": "No", "405 Shutter-Demo": "No", "528 AO-Name": "AnalogIO", "520-State": "0", "Core-Galvo": "", "405 Shutter-TriggerSampleRate": "100000", "Focus-FocusStepSize_(nm)": "100", "PRIME-PixelType": "16bit", "COM4-DataBits": "8", "470 AO-IOChannel": "Dev1/ao2", "520 AO-MinVolts": "0.0000", "COM9-Parity": "None", "PRIME-CircularBufferEnabled": "ON", "PRIME-PP 6 Reset": "No", "640 AO-Description": "NI DAC", "Source": "PRIME", "445 AO-MinVolts": "0.0000", "PIEZO-Name": "Stage", "445 AO-Description": "NI DAC", "PRIME-CircularBufferFrameCount": "46", "PRIME-PP 1 DESPECKLE BRIGHT HIGH": "DESPECKLE BRIGHT HIGH", "520 AO-Volts": "0.0000", "528 Shutter-TriggerSampleRate": "100000", "XPositionUm": 15712.1, "OpticalPath-State": "4", "PRIME-PP 3 THRESHOLD": "80", "PRIME-PVCAM Version": "3.7.1", "555 Shutter-TriggerSequenceLength": "1000", "PRIME-Gain": "1-HDR", "640 Shutter-TriggerSequenceLength": "1000", "528 AO-SupportsTriggering": "0", "445 AO-TriggerInputLine": "/Dev1/PFI0", "OpticalPath-Name": "OpticalPath", "PRIME-PP 4 ENABLED": "Yes", "PRIME-PVCAM Adapter Version": "1.3.29", "COM3-Name": "COM3", "405 AO-MaxVolts": "5.0000", "520 Shutter-Block voltage": "false", "PRIME-Timing-ClearingTimeNs": "49344000.0000", "Core-Focus": "PIEZO", "528 AO-Block voltage": "false", "TE2000-Description": "Nikon TE2000 hub", "528 AO-MinVolts": "0.0000", "COM4-Parity": "None", "Summary": { "Slices": 11, "ChContrastMin": [ 0, 0, 0, 0, 0, 0 ], "PixelAspect": 1, "SlicesFirst": true, "Interval_ms": 0, "Time": "2019-12-18 23:10:50 -0500", "Prefix": "seq_5", "InitialPositionList": [ { "Label": "Pos0", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.21], "Focus": [17741.5], "XYStage": [ 44542.200000000004, 31636.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos1", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.22], "Focus": [17741.4], "XYStage": [ 43801.100000000006, 31637.9 ] }, "GridColumnIndex": 0 }, { "Label": "Pos2", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.5], "XYStage": [ 38561.3, 32040.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos3", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.185], "Focus": [17741.3], "XYStage": [ 37698.4, 32041.7 ] }, "GridColumnIndex": 0 }, { "Label": "Pos4", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.16], "Focus": [17741.5], "XYStage": [ 32375.4, 31758.9 ] }, "GridColumnIndex": 0 }, { "Label": "Pos5", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.17000000000002], "Focus": [17741.3], "XYStage": [ 31770.7, 31758.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos6", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.4], "XYStage": [ 26536.800000000003, 31068.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos7", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.205], "Focus": [17741.4], "XYStage": [ 25420.2, 31068.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos8", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.85], "Focus": [17741.3], "XYStage": [ 21059, 31508 ] }, "GridColumnIndex": 0 }, { "Label": "Pos9", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.82], "Focus": [17741.3], "XYStage": [ 19731.9, 31506.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos10", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.79], "Focus": [17741.4], "XYStage": [ 14528.5, 32007.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos11", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.83], "Focus": [17741.2], "XYStage": [ 13830.300000000001, 32009.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos12", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.79], "Focus": [17741.3], "XYStage": [ 8775.300000000001, 32017.100000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos13", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.815], "Focus": [17741.3], "XYStage": [ 8109.200000000001, 32008.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos14", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.805], "Focus": [17741.3], "XYStage": [ 3483.3, 31908.2 ] }, "GridColumnIndex": 0 }, { "Label": "Pos15", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [248.82], "Focus": [17741.2], "XYStage": [ 2686.8, 31903.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos16", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [211.48000000000002], "Focus": [17741.1], "XYStage": [ -1978.9, 31973.600000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos17", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [211.51500000000001], "Focus": [17741.1], "XYStage": [ -2957.8, 31982.300000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos18", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.195], "Focus": [17741.5], "XYStage": [ 47747.4, -576.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos19", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.23000000000002], "Focus": [17741.6], "XYStage": [ 47053.9, -576.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos20", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.205], "Focus": [17741.4], "XYStage": [ 41871.200000000004, -479.8 ] }, "GridColumnIndex": 0 }, { "Label": "Pos21", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.19], "Focus": [17741.4], "XYStage": [ 40882.600000000006, -392 ] }, "GridColumnIndex": 0 }, { "Label": "Pos22", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.24], "Focus": [17741.4], "XYStage": [ 35905.6, -135.3 ] }, "GridColumnIndex": 0 }, { "Label": "Pos23", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [204.195], "Focus": [17741.5], "XYStage": [ 34990.9, -132.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos24", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.46], "Focus": [17741.4], "XYStage": [ 29678.100000000002, -958.1 ] }, "GridColumnIndex": 0 }, { "Label": "Pos25", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.44], "Focus": [17741.5], "XYStage": [ 28983.600000000002, -939.4000000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos26", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.475], "Focus": [17741.5], "XYStage": [ 23019.7, -122 ] }, "GridColumnIndex": 0 }, { "Label": "Pos27", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.44], "Focus": [17741.7], "XYStage": [ 22428, -116.80000000000001 ] }, "GridColumnIndex": 0 }, { "Label": "Pos28", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.46], "Focus": [17741.7], "XYStage": [ 16364.900000000001, -181.10000000000002 ] }, "GridColumnIndex": 0 }, { "Label": "Pos29", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.48000000000002], "Focus": [17741.5], "XYStage": [ 15712.1, -180 ] }, "GridColumnIndex": 0 }, { "Label": "Pos30", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.45000000000002], "Focus": [17741.7], "XYStage": [ 10346.1, 77.5 ] }, "GridColumnIndex": 0 }, { "Label": "Pos31", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.49], "Focus": [17741.4], "XYStage": [ 9755.5, 85.4 ] }, "GridColumnIndex": 0 }, { "Label": "Pos32", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.475], "Focus": [17741.5], "XYStage": [ 4775.5, 293.90000000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos33", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.445], "Focus": [17741.4], "XYStage": [ 3954.9, 293.90000000000003 ] }, "GridColumnIndex": 0 }, { "Label": "Pos34", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.455], "Focus": [17741.4], "XYStage": [ -1324.9, 545.6 ] }, "GridColumnIndex": 0 }, { "Label": "Pos35", "GridRowIndex": 0, "DeviceCoordinatesUm": { "PIEZO": [218.445], "Focus": [17741.3], "XYStage": [ -2097, 576.6 ] }, "GridColumnIndex": 0 } ], "z-step_um": 10, "GridRow": 0, "BitDepth": 16, "Depth": 2, "Channels": 6, "Source": "Micro-Manager", "ChColors": [ -16711681, -256, -65281, -1, -1, -16776961 ], "MetadataVersion": 10, "KeepShutterOpenChannels": true, "ChContrastMax": [ 65536, 65536, 65536, 65536, 65536, 65536 ], "CustomIntervals_ms": [], "Height": 1200, "MicroManagerVersion": "1.4.23 20180219", "GridColumn": 0, "UUID": "67598d21-5db5-4842-ab60-f506f7b42c7c", "PixelType": "GRAY16", "Width": 1200, "Directory": "F:\\Dropbox\\ZadorConfocal2data\\Yan\\20191218-YC113-S7S8-miseq", "Frames": 1, "Comment": "", "UserName": "Nikon", "PixelSize_um": 1.1, "ROI": [ 0, 0, 1200, 1200 ], "Date": "2019-12-18", "ChNames": [ "Il-G", "Il-T", "Il-A", "Il-C", "P-DIC", "P-DAPI-high" ], "KeepShutterOpenSlices": true, "IJType": 1, "ComputerName": "ZadorConfocal2", "Positions": 36, "PositionIndex": 29, "CameraTimeout": 20000, "TimeFirst": false }, "640 AO-Name": "AnalogIO", "Vincent-D3-Command": "Close" } }